Abstract:
The present invention provides a process for the expression in appropriate host cells of recombinant DNA which is under the control of an inducible promotor. In order to obtain the soluble form of recombinant protein, which would usually be obtained in insoluble form, the induction of the promotor is limited to less than 10% of the maximum induction in comparison with a standard system and the transcription rate of the DNA coding for the protein is correspondingly limited.

Description:
This application is a continuation of application Ser. No. 07/215,724, filed Jul. 6, 1988, now abandoned. 
    
    
     The present invention is concerned with a process for the expression of recombinant DNA which is under the control of an inducible promoter in a host cell. 
     By means of recombinant DNA technology, many problems in the production of proteins can be solved or considerably simplified. Briefly, the DNA sequence (gene) coding for the desired protein is integrated into an expression vector and expression of the protein is controlled via regulatory sequences, especially via promoters. 
     Especially preferred promotors, with which high yields of homologous and heterologous proteins can be obtained, are those which, as a rule, show both high and controllable activity. Such promotors include natural, hybrid and bacteriophage promotors, for example the lac, lacuv5, trp, tac, trc, rac, phoA, mgl, λ-P L , λ-P R , T 5 , T 7  and SP6 promoters, when the host cell is  Escherichia coli.    
     The activity of these promotors can be controlled, for example, by inducers (substrates, substrate analogues), by the temperature, or by the amount of available bacteriophage RNA polymerase. Using these, one can ensure, e.g., that heterologous protein is expressed only during particular periods of cultivation. For example, one can control expression so that it does not take place at the beginning of cultivation of the micro-organism used as host. Such control is especially advantageous when the heterologous protein is toxic for the host cell. When the expression of toxic heterologous proteins in hosts, such as  Escherichia coli,  is desired, it is usual to proceed in such a manner that, after a culturing phase in which the promotor is present in a repressed state, an appropriate inducer is added for the complete activation of the promotor. The promotor and thus the expression of the foreign protein is often induced in the late logarithmic to early stationary growth phase of the host cell after the achievement of an appropriate high cell density (biomass) (Mitzukamie et al., Biotech. Lett., 8, 611-614/1986). In the case of the use of the lac, lacuv5, tac, trc and rac promotor, as inducer there is added, for example, isopropyl-β-D-thiogalactopyranoside (IPTG) in a concentration of about 1 mM (Miller, Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1972). The expression of protein which is under lac repressor synthesis (lacI q ) increases with increasing inducer concentration (IPTG: 0, 2 and 20 mM) (Luck et al., DNA, 5, 21-28/1986). The repression of the trp promotor by tryptophan is removed by removal of the co-repressor tryptophan (tryptophan-free medium) and/or by induction with 3,β-indolylacrylic acid (IAA) in a concentration of 20 to 50 mg/l. (Smith et al., Gene, 32, 321-327/1984; Mosteller et al., Symp. Quant. Biol., 35, 461-466/1970). 
     However, it has been shown that this process does not: lead for all homologous and heterologous proteins to satisfactory yields of proteins of the desired properties,, such as solubility, natural conformation and possibly biological activity. Whereas for some enzymatically-active proteins, for example β-galactosidase, β-lactamase, chloramphenicol acetyltransferase (CAT), creatinase and penicillin acylase, solubility and high enzyme activity are achieved after expression in  Escherichia coli,  with the use of the same conditions and of the same promotors, in the case of other proteins only small amounts of soluble proteins or no soluble protein at all or even biologically inactive product are obtained. Such effects are observed, for example, in the case of α-glucosidase, prochymosin, the heavy and light chains of antibodies, immunoglobulins, urokinase, tPA, interferon, growth hormone, fusion proteins from β-galactosidase and HIV antigens and human insulin polypeptides (Marston, Biochem. J., 240, 1-12/1986). 
     However, it has been shown that these proteins are certainly present in large amounts in the appropriate cell extracts but are insoluble and/or display no biological activity. 
     As the cause for the low expression of soluble and especially of active protein in  Escherichia coli,  there has been discussed, for example, the denaturing of the foreign protein to give refractile bodies (inclusion bodies), i.e. to give insoluble protein aggregates, as well as proteolysis of the foreign gene product by the cell&#39;s own proteases (Carriers et al., Trends in Biotechnology, 1, 109-113/1983). In order here to provide some help, it has been proposed by Mitzukamie et al. (Biotech. Lett., 8, 605-610/1986), that for the expression of beta interferon (IFN-β) in  Escherichia coli  under the control of the trp promotor, to carry out the fermentation at 20° C. At this temperature, the yield of active IFN-β increases. However, it is a disadvantage of this process that fermentation times of at least 4 days are needed in order to obtain activities for which the working up is worthwhile. 
     Furthermore, it has been suggested to use host cells from which the proteolytic system responsible for the cleavage of the gene product is absent. Thus, for example, a lon mutant of  Escherichia coli  can be used as host cell. Such mutants are deficient with regard to a protease occurring in the wild type. Since, however, at least 7 further proteases are present in  Escherichia coli  (Swamy et al., J. Bacteriol., 149, 1027-1033/1982), the process is then only appropriate when the protein to be expressed is not cleaved by these other proteases. Furthermore, the choice of appropriate host cells is very limited. 
     Furthermore, it has been suggested to increase the expression by increasing the copy number of the expression vector to such an extent that the proteases are inundated by large amounts of protein formed and, consequently, only inactivate a comparatively small percentage of the protein. However, a disadvantage of this method is that the expression must be increased immensely within only 1 or 2 generations and cannot be carried out for a comparatively long period of time (Trends in Biotechnology, 1, 109-113/1983). 
     Therefore, it is an object of the present invention to avoid the above-described disadvantages of the prior art and to make possible the production of soluble and biologically-active proteins. 
     Thus, according to the present invention, there is provided a process for the expression in appropriate host cells of recombinant DNA which is under the control of an inducible promotor, wherein, for the production in soluble form of proteins which, under usual gene-technological production conditions, are obtained in insoluble form, the induction of the promotor is limited to less than 10% of the maximum induction in comparison with a standard system , which results a limited transcription rate of the DNA coding for the protein and a reduced rate of the synthesis of said protein. 
     By the limitation of the induction of the promotor, it is possible to limit the formation of refractile bodies and, instead, to produce soluble and active protein in large amounts. Without such a limitation of the speed of expression, complete induction admittedly leads to very rapid formation of protein. These proteins, however are present in the form of refractile bodies or as inactive proteins or protein fragments. 
     In the case of the process according to the present invention, the induction of the promotor is preferably limited to less than 5% and an especially preferred embodiment limits it to less than 1% of the maximum induction in comparison with a standard system. 
     As standard system, one may use a vector in which the foreign gene to be expressed has been replaced by another heterologous or homologous gene which, in this system (equal to the host cell), expresses soluble, active protein, in which case this “standard gene” must then, of course, be under the control of the same promotor as the foreign gene. Examples of standard gene includes β-lactamase, CAT and creatinase. As another standard system, the host cell itself (without vector) could also be used when it already contains, chromosomally integrated, a standard gene under comparable control of the promotor intended for use (see the following Example 2, the β-galactosidase gene of  Escherichia coli  encoded by the natural lac-operon. 
     All host cells in which the expression of a recombinant DNA under the control of an inducible promotor is possible can be used for the expression of the recombinant DNA. The expression is preferably carried out in  Escherichia coli  and especially preferably in  Escherichia coli  strains which carry a lac-I q  gene. An example is  Escherichia coli,  DSM 2102, which is transformed with plasmid pePA 119 (DMS 3691P). In the following description, this strain is referred to as ED82-I q . The induction thereby takes place most favorably in the logarithmic growth phase. As promoters for the process according to the present invention, in  Escherichia coli  there can be used natural, hybrid and bacteriophage promotors. Preferably, there are used the lac, lacuv5, trp, tac, trc, rac, phoA, mgl, λ-P L , λ-P R , T 5 , T 7  and SP6 promotors. 
     A maximum induction of β-galactosidase in  Escherichia coli  wild type cells (lacI + Z + Y + ) is achieved with an inducer concentration of 0.1 to 1 mM IPTG (R. Knippers, Molekulare Genetik, pub. Georg Thieme Verlag, Stuttgart, New York, 1985). In the presence of an intact lactose permease (lacY + ), IPTG is actively transported into the cell at a concentration more than 50 fold of the extracellular concentration so that, intracellularly there exists an inducer concentration of more than 5 mM (Miller, Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1972). 
     According to a preferred embodiment of the present invention, limited induction is brought about by lowering the effective intracellular inducer concentration less than 10%, preferably to less than 5% and especially to less than 1% in comparison with a standard system as defined above. Maximum induction is then achieved when, after doubling of the inducer concentration, the specific enzyme activity of the standard increases less than 15%. 
     The limitation of the induction can be brought about, for example, by limited addition of inducer. In the case of the use of the lac promotor or of a promotor derived therefrom, as inducer there is added IPTG, preferably in a concentration of less than 0.01 mM. 
     According to a further preferred embodiment, the limitation of the induction takes place by limitation of the active transport of the inducer through the cell membrane. A defective transport system can, for example, be obtained by making a mutation in the lactose permease gene (lacY) of  Escherichia coli.  In this case, as inducer there can be used, for example, IPTG or lactose. In the case of a defective transport system, the intracellular IPTG concentration then corresponds to the extracellular concentration (Miller, Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1972). In spite of defective lactose permease, lactose can be taken up to a small extent via the thiomethylgalactosidase I and arabinose permeation system (Rotman et al., J. Mol. Biol. 36, 247-260/1968, Messer, J. Bacteriol. 120, 266-272/1974). 
     There is preferably used the lac promotor or a promotor derived therefrom in a host cell which carries a mutation in this lactose permease gene (lacy). 
     As host cells, there are especially preferably used cell of  Escherichia coli,  which contain a defective lactose permease gene and, in addition, the lac-I q  gene, for example,  Escherichia coli  strain ED82-I q , DSM 2102, transformed with plasmid DSM 3691P, which encodes for the lac-I q  gene. For induction lactose is added to the culture medium in a concentration of less than 1%. The lactose present in the medium is scarcely metabolized but it suffices for the induction of the promotor. 
     The limitation of the induction can also be achieved by the addition of inducers, which are metabolized by the cells and thus lose their inducing action. 
     A further embodiment of the limitation of the induction in the process according to the present invention involves controlling the activity of promoters by influencing the effector-promotor exchange action. The intracellular concentration of the inducer is thereby not decisive but its affinity for the repressor or vice versa controls. Lowering of the inducer-repressor exchange action can, for example, be achieved by the use of a micro-organism which carries a repressor mutant gene, which encodes for a repressor-protein with a changed repressor-inducer binding constant. In such a process, the lac promotor is preferred, as well as a micro-organism which carries a lac-repressor mutant gene (lacI s ). 
     A further embodiment uses promoters which possess a catabolite-activator protein (CAP) site, the affinity of the catabolite activator protein to the promotor thereby being reduced. 
     The effect according to the present invention can be increased by additional measures which affect the growth of the cells in the case of the fermentation and by the point of time at which the inducer is added. Thus, the expression of soluble, active α-glucosidase takes place by very slow induction of the tac promotor and additional increasing measures which influence the growth. 
     The speed of growth of cells can be influenced, for example, by the nature of the medium (complete or minimal media, for example DYT, LB, M9CAA and M9), the carbon source (for example sugar, glycerol and peptone), the nitrogen source (for example ammonium sulphate, amino acids and peptone), the pH value of the medium, the temperature, the amount of available oxygen, as well as the origin of the strain (mutations). Changed growth rates can also be broughtabout by overlapping effects. Thus, for example, by decomposition of carbon sources, the medium becomes strongly acidic (pH 4.8) resulting in slowed growth rates. 
    
    
     BRIEF DESCRIPTION OF THE FIGURES 
     FIG. 1 is the nucleotide sequence and amino acid sequence derived therefrom of α-glucosidase pI; and 
     FIG. 2 is the construction of the α-glucosidase pI plasmid pKK177-3 /GLUCPI for expression in  Escherichia coli.    
    
    
     DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS 
     Expression of α-glucosidase in  Escherichia coli    
     For the manipulation of DNA, standard methods are used such as are described by Maniatis et al.(1982) in Molecular Cloning, (Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. 11724). The molecular biological reagents used are employed according to the instructions of the manufacturer. 
     EXAMPLE 1 
     Construction of the α-glucosidase pI expression vector pKK177 3/GLUCPI 
     The structural gene of α-glucosidase pI (FIG. 1) from Bakers&#39; yeast is constructed via an adaptor (correct binding between promotor and N-terminus) and two α-glucosidase-coding DNA fragments of the plasmid YRp/GLUCPI, DSM 4173P (FIG.  2 ). The 3′-untranslated region of the α-glucosidase from yeast is removed up to 25 bp. 
     For this purpose, the plasmid YRp/GLUCPI is digested with EcoRI and BclI and the BclI/EcoRI fragment (1.7 kb), as well as the EcoRI fragment (about 0.3 kb), isolated. The EcoRI fragment is post-cleaved with FnuDII and the EcoRI/FnuDII fragment (0.13 kb) isolated. 
     The vector pKK177-3 DSM 3062, is cleaved with EcoRI and SmaI. Into the resulting vector fragment (2.85 kb) the BclI/EcoRI fragment the EcoRI/FnuDII fragment, as well as the synthetic DNA fragment 
     5′-AATTCCATGACTATTTCT -3′ 
     3′-GGTACTGATAAAGACTAG -5′ 
     are ligated. 
     The desired construction was identified and isolated on 5-bromo-4-chloroindolyl-α-D-glucopyranoside indicator plates α-XGl, 40 mg/liter) and 1 mM IPTG on the basis of low α-glucosidase activity in ED82-I q . 
     The correct construction of the α- GLUCPI gene is confirmed by restriction analysis. The plasmid has the designation pKK177-3/GLUCPI. The host strain ED82-I q  has no α-glucosidase activity under the test conditions. 
     EXAMPLE 2 
     Expression of yeast α-glucosidase PI in  Escherichia coli  under standard conditions 
     For the heterologous expression of α-glucosidase pI from yeast, there was used the  Escherichia coli  K-12 strain ED82-I q  which contains the vector pKK177-3/GLUCPI. The experiments were carried out in roller cultures (20 ml reagent glass with 5 ml of medium) in LB medium (Maniatis et al., Molecular Cloning, Cold Spring Harbor Laboratory, 1982) with 40mg/liter of ampicillin at 37° C. The cultures were inoculated with 50 μl of overnight culture and, upon achieving a cell density of OD 550  of from 0.5 to 0.6, were induced with 5 mM IPTG (end-concentration). After 2 to 3 hours 0,5 ml culture samples were harvested, the cell pellets washed with 10 mM phosphate buffer pH 6.8 and immediately frozen. 
     α-glucosidase and β-galactosidase assay 
     Frozen all pellets were resuspended in 0.25 ml 10 mM phosphate buffer pH 6.8 with 1 mM EDTA and disrupted by sonification. After centrifugation soluble active α-glucosidase was assayed in the supernatant in 0.1 M phosphate buffer pH 6.8 at 25° C. with 2mM p-nitrophenyl-α-D-glucopyranoside (PNPG) as substrat. For the calculation of spezific activities the protein was estimated according to the micro-biuret method (Zamenhof, Methods Enzymol. 696-704/1957) with bovine serum albumin as a standard. Specific activities are expressed as nanomoles of substrate hydrolyzed per minute per milligram of protein. The β-galactosidase was determined analogously using 2-nitrophenyl-β-D-galactopyranoside as substrate instead of PNPG. The chromosomally coded β-galactosidase in ED82-I q  served for the control of the course of induction of the lac operon. 
     EXAMPLE 3 
     a) Dependency of the yield of active protein (α-glucosidase pI and β-galactosidase) depending upon the period of induction and upon the IPTG inducer concentration. 
     Working was carried out as described in Example 2, the inducer concentration (IPTG) and the period of culturing thereby being varied. The results obtained are shown in the following Table I. 
     
       
         
               
             
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Yield of active protein (mU/mg protein) 
               
               
                 Fermentation period (min) 
               
             
          
           
               
                 inducer 
                   
                 450 
               
             
          
           
               
                 IPTG 
                 40 
                 85 
                 145 
                 α- 
                   
               
             
          
           
               
                 (mM) 
                 α-Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
                 Gluc 
                 β-Gal 
               
               
                   
               
             
          
           
               
                 0 
                 175 
                 1 
                 132 
                 2 
                 140 
                 12 
                 11 
                 17 
               
               
                 0.01 
                 193 
                 2 
                 194 
                 6 
                 156 
                 15 
                 8 
                 46 
               
               
                 0.033 
                 120 
                 6 
                 152 
                 16 
                 123 
                 27 
                 7 
                 57 
               
               
                 0.066 
                 83 
                 12 
                 113 
                 27 
                 102 
                 33 
                 7 
                 85 
               
               
                 0.1 
                 82 
                 24 
                 102 
                 36 
                 106 
                 47 
                 6 
                 140 
               
               
                 0.33 
                 60 
                 48 
                 86 
                 67 
                 102 
                 100 
                 12 
                 281 
               
               
                 0.66 
                 48 
                 50 
                 77 
                 83 
                 100 
                 116 
                 10 
                 334 
               
               
                 1 
                 58 
                 66 
                 80 
                 96 
                 88 
                 117 
                 9 
                 384 
               
               
                 3 
                 62 
                 76 
                 78 
                 95 
                 90 
                 116 
                 9 
                 405 
               
               
                 6 
                 68 
                 74 
                 84 
                 114 
                 97 
                 135 
                 6 
                 436 
               
               
                   
               
             
          
         
       
     
     The results show that the specific activity of the β-galactosidase (internal control) continuously increases with increasing inducer concentration and increasing period of induction. In contradistinction to β-galactosidase, the specific activity of the β-glucosidase achieves a maximum at a concentration of 0.01 mM of IPTG. 
     b) Dependency of the yield of active protein (α-glucosidase and β-galactosidase) upon the lactose inducer concentration 
     Working was carried out as described in Example 2, the inducer concentration (lactose) thereby being varied. The results obtained are shown in the following Table II. 
     
       
         
               
               
               
             
               
               
               
             
               
               
               
             
           
               
                 TABLE II 
               
             
             
               
                   
               
               
                   
                 yield of active protein 
                   
               
               
                 inducer 
                 (mU/mg protein) 
               
             
          
           
               
                 lactose (%) 
                 α-Gluc 
                 β-Gal 
               
               
                   
               
             
          
           
               
                 0 
                 54 
                 1 
               
               
                 0.05 
                 110 
                 5 
               
               
                 0.1 
                 134 
                 9 
               
               
                 0.5 
                 199 
                 36 
               
               
                 1 
                 223 
                 38 
               
               
                 2 
                 13 
                 1080 
               
               
                   
               
             
          
         
       
     
     The results show that the β-galactosidase is fully induced (internal control) by a lactose concentration of 2%. In contradistinction thereto, in the case of the same inducer concentration, the α-glucosidase only achieves a specific activity of 5% of the maximum achievable specific enzyme activity. 
     EXAMPLE 4 
     Dependency of the yield of active protein (α-glucosidase and β-galactosidase) on the pH value and upon the concentration of inducer IPTG 
     Working was carried out as described in Example 2, the pH value at the time of induction being adjusted by the addition of Tris-HCl or phosphate buffer (end concentration 0.1 M) and an inducer concentration of 0.01 mM IPTG (Table IIIa) and 0.5% lactose (Table IIIb) being used. The results obtained are given in the following Tables IIIa and IIIb. 
     
       
         
               
               
               
             
               
               
               
             
               
               
               
               
             
           
               
                   
                 TABLE III 
               
             
             
               
                   
                   
               
               
                   
                 yield of active protein 
                   
               
               
                   
                 (mU/mg protein) 
               
             
          
           
               
                   
                 α-Gluc 
                 β-Gal 
               
               
                   
                   
               
             
          
           
               
                   
                 a) pH value 
                   
                   
               
               
                   
                 inducer 
               
               
                   
                 IPTG 0.01 (mM) 
               
               
                   
                 8.7 
                 3 
                 — 
               
               
                   
                 8.0 
                 168 
                 1 
               
               
                   
                 7.0 
                 53 
                 1 
               
               
                   
                 6.3 
                 79 
                 1 
               
               
                   
                 6.0 
                 264 
                 1 
               
               
                   
                 5.6 
                 384 
                 1 
               
               
                   
                 b) inducer 
               
               
                   
                 lactose 
               
               
                   
                 (0.5%) 
               
               
                   
                 8.0 
                 179 
                 14 
               
               
                   
                 7.0 
                 346 
                 17 
               
               
                   
                 6.0 
                 867 
                 17 
               
               
                   
                 5.5 
                 969 
                 19 
               
               
                   
                 5.0 
                 1629 
                 5 
               
               
                   
                   
               
             
          
         
       
     
     It can be seen that at the pH range optimal for culturing  Escherichia coli  (7.0 to 7.5), there is surprisingly obtained the lowest yield of active protein. The optimum ranges are from 4.8 to 5.6, as well as from 7.5 to 8.5. Furthermore, the enzyme activity is increased by a factor of 8 in comparison with the sole induction with lactose (0.5%). (Table II, line 4, compared with Table IIIb), line 5). 
     EXAMPLE 5 
     Dependency of the yield of active protein (α-glucosidase and β-galactosidase) upon the culturing temperature 
     Working was carried out as described in Example 2, the temperature and the inducer IPTG (0.01 mM) and lactose (0.5%) being varied. The results are given in the following Table IV. 
     
       
         
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE IV 
               
             
             
               
                   
                   
               
               
                   
                 yield of active protein (mU/mg protein) 
               
               
                   
                 temperature (° C.) 
               
             
          
           
               
                   
                 22 
                   
                   
                   
                   
               
               
                   
                 α- 
                   
                 27 
                 32 
                 37 
               
             
          
           
               
                 inducer 
                 Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
               
               
                   
               
               
                 IPTG 
                 n.d. 
                 n.d. 
                 1500 
                 16 
                  935 
                 24 
                 125 
                 75 
               
               
                 (mM) 
               
               
                 0.06 
               
               
                 lactose 
                 2200 
                 6 
                 1560 
                  5 
                 1050 
                  9 
                 460 
                 22 
               
               
                 (%) 
               
               
                 0.5 
               
               
                   
               
               
                 n.d. = not determined  
               
             
          
         
       
     
     The results show that the specific activity of the β-galactosidase (internal control) is not influenced by the culturing temperature. Surprisingly, however, the specific activity of the α-glucosidase increases in the case of lower culturing temperatures. 
     EXAMPLE 6 
     Dependency of the yield of active protein (α-glucosidase and β-galactosidase) upon the medium and period of culturing 
     Working was carried out as described in Example 2, the medium being varied and 0.5% lactose being used as inducer. The results obtained are shown in the following Table V. 
     
       
         
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE V 
               
             
             
               
                   
                   
               
               
                   
                 yield of active protein 
               
               
                   
                 (mU/mg protein) 
               
               
                   
                 culture period (h ) 
               
             
          
           
               
                   
                 3 
                 6 
                 20 
               
             
          
           
               
                 Medium 
                 α-Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
               
               
                   
               
             
          
           
               
                 LB 
                 600 
                 4 
                 1500 
                 13 
                 4 
                 124 
               
               
                 M9CAA** 
                 30 
                 — 
                 110 
                 4 
                 2000 
                 113 
               
               
                 M9* 
                 10 
                 — 
                 20 
                 — 
                 3000 
                 83 
               
               
                   
               
               
                 *M9 minimal medium per-liter:  
               
               
                 Na 2 HPO 4 6 g.    
               
               
                 KH 2 PO 4  3 g.  
               
               
                 NaCl 0.5 g.  
               
               
                 NH 4 Cl 1 g.  
               
               
                 MgSo 4 .7H 2 O 1 mM  
               
               
                 thiamine 1 mg/l.  
               
               
                 glucose 0.2%  
               
               
                 **M9CAA M9CAA minimal medium M9 with 0.5% casamino acids  
               
             
          
         
       
     
     EXAMPLE 7 
     Dependency of the yield of active protein (α-glucosidase and β-galactosidase) upon the carbon source 
     
       
         
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
                 TABLE VI 
               
             
             
               
                   
                   
               
               
                   
                 yield of active protein 
                   
               
               
                   
                 (mU/mg protein) 
               
               
                   
                 culture period (h ) 
               
             
          
           
               
                   
                 carbon 
                 2.5 
                 6 
                   
               
             
          
           
               
                   
                 source 
                 α-Gluc 
                 β-Gal 
                 α-Gluc 
                 β-Gal 
               
               
                   
                   
               
             
          
           
               
                   
                 glucose 
                 414 
                 — 
                 649 
                 3 
               
               
                   
                 maltose 
                 1535 
                 7 
                 1982 
                 7 
               
               
                   
                 glycerol 
                 833 
                 12 
                 2838 
                 14 
               
               
                   
                 maltose/glycerol 
                 1312 
                 12 
                 1744 
                 9 
               
               
                   
                   
               
             
          
         
       
     
     EXAMPLE 8 
     Preferred culturing conditions for active α-glucosidase 
     For the synthesis of active α-glucosidase, ED 82 -I q  with plasmid-coded α-glucosidase was cultured in LB medium or in minimal medium at 37° C. up to an OD 550  of 0.4 to 0.6. Thereafter, the culture was cooled (20 to 30° C.), induced with lactose (end concentration 0.5%) and either a carbon source (end concentration 1 to 2%; preferably glycerol and/or maltose) added thereto or the pH value lowered with phosphate buffer (0.1 M) to pH 4.8 to 5.5 and the cells cultured at 20 to 30° C. up to a cell density of OD 550  of 3 to 5. The results obtained are given in the following Table VII. 
     
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE VII 
               
             
             
               
                   
               
               
                   
                   
                   
                   
                   
                 yield of 
               
               
                   
                 carbon 
                 culture 
                   
                 inducer 
                 active protein 
               
               
                   
                 source 
                 time 
                 temperature 
                 lactose 
                 (mU/mg protein) 
               
             
          
           
               
                 medium 
                 (%) 
                 (h) 
                 (° C.) 
                 (%) 
                 α-Gluc 
                 β-Gal 
               
               
                   
               
               
                 LB 
                 glycerol 
                 20 
                 27 
                 0.5 
                 5500 
                 — 
               
               
                 LB 
                 glycerol 
                 20 
                 37 
                 0.5 
                 5000 
                 — 
               
               
                 LB 
                 — 
                  6 
                 27 
                 0.5 
                 4000 
                 110 
               
               
                 M9 
                 — 
                 20 
                 37 
                 0.5 
                 3500 
                 110 
               
               
                 M9 
                 glycerol 
                 20 
                 37 
                 0.5 
                 4000 
                 n.d. 
               
               
                 M9 
                 gly/mal 
                 20 
                 37 
                 0.5 
                 3900 
                 n.d. 
               
               
                   
               
               
                 n.d. = not determined  
               
             
          
         
       
     
     It will be understood that the specification and examples are illustrative but not limitative of the present invention and that other embodiments within the spirit and scope of the invention will suggest themselves to those skilled in the art. 
     A deposit was made under the terms of the Budapest Treaty of the following materials with the Deutsche Sammlung von Mikroorganismen, Mascheroder Weg 1B, 3300 Braunschweig, Germany. The deposited materials are listed with their accession numbers and submission: 
       Escherichia coli  I q  (DSM 2102) (May 27, 1981) 
     vector pKK 177/3 (DSM 3062) (Sep. 26, 1984) 
     plasmid pePA119 (DSM 3691) (Apr. 9, 1986) 
     plasmid Yrp-Gluc.pI (DSM 4173P) (Jun. 29, 1987)