Abstract:
A family of somatotropin vectors with which micro-organisms, preferably bacteria such as, but not limited to  E. coli , can be transformed to enable the expression of bovine somatotropin at high levels using conventional fermentation and induction conditions.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     In accordance with 35 U.S.C. §§119(e) and 365(c) this continuation-in-part application claims the benefit of the filing date of co-pending international application PCT/US01/49977, with an international filing date of Dec. 21, 2001, which claims the benefit of U.S. provisional application No. 60/258,291, filed Dec. 26, 2000, the entirety of each of which is herein incorporated by reference. 
    
    
     BACKGROUND OF THE INVENTION 
     In mammals, growth hormones (somatotropins) are initially expressed in a pre-hormone form comprising a leader sequence which is removed during secretion of the mature, biologically active hormone from pituitary cells. As used herein, “mature” somatotropin (“ST”) means having an amino acid length essentially like those of the ST secreted into the bloodstream of the animal, e.g., N-Ala-Phe-Pro- or N-Phe-Pro-bovine ST, N-Phe-Pro-human, -porcine (identical to canine) or -equine ST, etc. 
     Attempts to bacterially express human and bovine STs in non-secretion systems using structural genes having the sequences of their cDNAs were at first unsuccessful. Researchers succeeded after introducing silent mutations into the front end of the structural gene. P. H. Seeburg et al., attributed the original difficulties to translation being impeded by secondary structure of the mRNA corresponding to cDNA, and taught lessening such secondary structure to enable significant expression. DNA, Vol. 2, No. 1, 1983, pp. 37-45; U.S. Pat. Nos. 5,254,463 and 5,260,201. 
     It has been possible to obtain commercially viable ST expression levels using DNA containing certain silent mutations. However, the need for new vectors that are highly productive and less expensive to use has been difficult to satisfy because the relationships between the configurations and expression levels of “mature” somatotropin vectors remain poorly understood and highly unpredictable. 
     SUMMARY OF THE INVENTION 
     Described herein is a family of ST vectors with which micro-organisms, preferably bacteria such as, but not limited to  E. coli , can be transformed to enable the expression of bovine ST (“bST”) at high levels using conventional fermentation and induction conditions. This family is exemplified by the 44 vectors listed in Table I each of which comprises the corresponding sequence of SEQ ID NOs 1 through 44, as indicated in Table I. Each of SEQ ID NOs 1 through 44, extends from an EcoRI site (GAATTC) before the promoter region, through the promoter, ribosome binding site (“RBS”) and entire bST structural gene, ending with the translation stop codon for the structural gene. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIGS.  1 A/ 1 B: DNA sequence comparison of fragments of the expression vectors pXT674 (SEQ ID NO:49) and pXT709 (SEQ ID NO:50). 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Each of the vectors having SEQ ID NOs 1-44 includes a synthetic promoter, herein designated “cpex-20”, which is disclosed in PCT Appln. PCT/US00/40014 filed Mar. 31, 2000, which claims priority of U.S. Provisional Patent Appln. No. 60/127,449, filed Apr. 1, 1999. This promoter is situated between the EcoRI site (GAATTC) and the AscI site (GGCGCGCC). Various other conventional and novel promoters can be used in these vectors in lieu of cpex-20 to achieve good levels of ST expression. 
     To construct the expression vector pXT709, the synthetic EcoRI-HindIII DNA fragment shown in FIGS.  1 A/ 1 B: (SEQ ID NO:50) was ligated into pBR322 (Boehringer Mannheim, Indianapolis, Ind.) which had been digested with EcoRI and HindIII. The resulting plasmid, designated pXT709, was then transformed into recA+  E. coli  K-12 host strain and somatotropin production was induced with nalidixic acid. 
     To construct similar plasmids comprising the other sequences listed in the Sequence Listing (namely SEQ ID NOs 1-15, and 17-44), the corresponding synthetic DNA fragments could be similarly prepared and ligated into pBR322. Furthermore, the many other techniques and/or procedures for ligating the described DNA fragments, as shown in the Sequence Listing, into various other plasmids are known and routinely practiced by those of ordinary skill in the art. Moreover, plasmids suitable for transforming  E. coli  and/or other bacteria so as to achieve somatotropin production from cultures of the transformed bacteria are known and regularly used by ordinarily skilled artisans. 
     SEQ ID NOs 1-17 have an identical RBS, CATAACATCAAGAGGATATGAAATT (SEQ ID NO:45), herein designated “dps”, that is native to  E. coli . SEQ ID NOs 18-29 share a novel synthetic RBS, GTACAAATCATAGAGGGTATTTAAT (SEQ ID NO:46) which is herein designated “L49”. SEQ ID NOs 30-42 share a second novel synthetic RBS, AGACACTAAATAGAGGGTATTTAATT (SEQ ID NO:47) herein designated “L437”. Seq. Nos. 43-44 share a third novel synthetic RBS, TAAAAGAGACTAGGAGGAGATTAGA (SEQ ID NO:48), herein designated “R806”. Each RBS is situated between the AscI site and the ATG translation start codon of the bST structural gene. The AscI site begins at coordinate 76 of each sequence. The ATG start codon begins at approximately coordinate 109; this can vary slightly due to the RBSs employed having slightly different lengths. 
     All of the vectors listed in Table I (respectively comprising SEQ ID NOS 1-44) have structural genes that differ from cDNA encoding “mature” bST by containing various arrays of silent changes within the nucleotides encoding the first 16 codons of bST. As shown in FIGS.  1 A/ 1 B, vector pXT709 (SEQ ID NO: 50) differs from pXT674 (SEQ ID NO: 49) by having an additional 12 codons within the structural gene changed from those of cDNA, 11 to  E. coli -preferred codons and a twelfth to eliminate an undesirable restriction site, by changing the translation stop codon to two tandem stop codons, and by substituting a different terminator which has two lacUV5 promoters in tandem. pXT703 (comprising SEQ ID NO:15) and pXT747 (comprising SEQ ID NO:17) differ from pXT601 (comprising SEQ ID NO:2) and pXT686 (comprising SEQ ID NO:13), respectively, in the same way. 
     Each of the vectors comprising SEQ ID NOs 1 through 44 have expressed mature bST at unoptimized levels of at least about 3.5 grams/liter (“gm/l”), most of them above 4.5 gm/l, and some at 7 gm/l or higher, using the fermentation conditions described in Bogosian et al., J. Biol. Chem., vol. 264, pp. 531-39, Jan. 5, 1989, except that 50 ppm of nalidixic acid was used as the inducer instead of indole acrylic acid. 
     The expression levels of mature ST vectors are very unpredictable from published teachings, e.g., P. H. Seeburg et al. In fact, the vectors comprising the sequences of SEQ ID NOs 1 through 17, 30 through 35, and 37 through 42 have been found, using calculations as recommended in Seeburg et al., to have mRNA secondary structure greater than, rather than lessened from that of bST cDNA. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE I 
               
               
                   
               
               
                 SEQ ID NO 
                 RBS 
                 pXT 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 1 
                 dps 
                 600 
               
               
                 2 
                 dps 
                 601 
               
               
                 3 
                 dps 
                 602 
               
               
                 4 
                 dps 
                 658 
               
               
                 5 
                 dps 
                 670 
               
               
                 6 
                 dps 
                 672 
               
               
                 7 
                 dps 
                 673 
               
               
                 8 
                 dps 
                 674 
               
               
                 9 
                 dps 
                 675 
               
               
                 10 
                 dps 
                 676 
               
               
                 11 
                 dps 
                 682 
               
               
                 12 
                 dps 
                 683 
               
               
                 13 
                 dps 
                 686 
               
               
                 14 
                 dps 
                 689 
               
               
                 15 
                 dps 
                 703 
               
               
                 16 
                 dps 
                 709 
               
               
                 17 
                 dps 
                 747 
               
               
                 18 
                 L49 
                 487 
               
               
                 19 
                 L49 
                 536 
               
               
                 20 
                 L49 
                 541 
               
               
                 21 
                 L49 
                 542 
               
               
                 22 
                 L49 
                 548 
               
               
                 23 
                 L49 
                 549 
               
               
                 24 
                 L49 
                 562 
               
               
                 25 
                 L49 
                 576 
               
               
                 26 
                 L49 
                 579 
               
               
                 27 
                 L49 
                 580 
               
               
                 28 
                 L49 
                 581 
               
               
                 29 
                 L49 
                 584 
               
               
                 30 
                 L437 
                 491 
               
               
                 31 
                 L437 
                 529 
               
               
                 32 
                 L437 
                 530 
               
               
                 33 
                 L437 
                 619 
               
               
                 34 
                 L437 
                 605 
               
               
                 35 
                 L437 
                 606 
               
               
                 36 
                 L437 
                 607 
               
               
                 37 
                 L437 
                 620 
               
               
                 38 
                 L437 
                 631 
               
               
                 39 
                 L437 
                 632 
               
               
                 40 
                 L437 
                 633 
               
               
                 41 
                 L437 
                 635 
               
               
                 42 
                 L437 
                 681 
               
               
                 43 
                 R806 
                 483 
               
               
                 44 
                 R806 
                 564 
               
               
                   
               
             
          
         
       
     
     
       
         
           
             50  
           
           
             1  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 600  
             
           
            1 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagca    120 
atgagcttgt ccggactttt tgcaaatgct gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             2  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 601  
             
           
            2 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgtcattgt ccggactgtt tgcgaacgct gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             3  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT602  
             
           
            3 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttccctgca    120 
atgtcattgt ccggactgtt tgcaaatgcg gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             4  
             684  
             DNA  
             Artificial sequence  
             
               Somatotropin expression vector pXT 658  
             
           
            4 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagca    120 
atgtcattgt ccggactctt tgcaaatgcg gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             5  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 670  
             
           
            5 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgagcttgt ccggattgtt tgcaaacgct gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             6  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 672  
             
           
            6 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagcc    120 
atgtcattgt ccggactgtt tgcaaatgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             7  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 673  
             
           
            7 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagcc    120 
atgagcttgt ccggactgtt tgccaacgct gtcctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             8  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 674  
             
           
            8 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgagcttgt ccggactctt tgccaatgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             9  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 675  
             
           
            9 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagcc    120 
atgagcttgt ccggactgtt tgccaatgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             10  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 676  
             
           
            10 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgtccttgt ccggactctt tgccaacgct gtcctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             11  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 682  
             
           
            11 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagcc    120 
atgtcattgt ccggactgtt tgcgaacgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             12  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 683  
             
           
            12 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagcc    120 
atgtcattgt ccggactctt tgcaaatgct gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             13  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 686  
             
           
            13 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagcc    120 
atgtcattgt ccggactctt tgcgaacgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             14  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 689  
             
           
            14 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgtcattgt ccggactgtt tgcaaacgct gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             15  
             687  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 703  
             
           
            15 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgtcattgt ccggactgtt tgcgaacgct gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gcgttactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc gacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
agctggcttg ggccgctgca gttcctcagc cgtgtcttca ccaacagctt ggtgtttggc    420 
accagcgacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgtgagctgg aagatggcac cccgcgtgct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccgtaagg acctgcataa gacggagacg tacctgcgtg tcatgaagtg ccgccgcttc    660 
ggggaggcga gctgcgcctt ctaatag                                        687 
 
           
             16  
             687  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 709  
             
           
            16 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgagcttgt ccggactctt tgccaatgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gcgttactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc gacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
agctggcttg ggccgctgca gttcctcagc cgtgtcttca ccaacagctt ggtgtttggc    420 
accagcgacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgtgagctgg aagatggcac cccgcgtgct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccgtaagg acctgcataa gacggagacg tacctgcgtg tcatgaagtg ccgccgcttc    660 
ggggaggcga gctgcgcctt ctaatag                                        687 
 
           
             17  
             687  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 747  
             
           
            17 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gttcccagcc    120 
atgtcattgt ccggactctt tgcgaacgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gcgttactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc gacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
agctggcttg ggccgctgca gttcctcagc cgtgtcttca ccaacagctt ggtgtttggc    420 
accagcgacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgtgagctgg aagatggcac cccgcgtgct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccgtaagg acctgcataa gacggagacg tacctgcgtg tcatgaagtg ccgccgcttc    660 
ggggaggcga gctgcgcctt ctaatag                                        687 
 
           
             18  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 487  
             
           
            18 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttcctgcg    120 
atgtcattgt ccggattgtt tgcgaatgcg gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             19  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 536  
             
           
            19 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagca    120 
atgtcattgt ccggattgtt tgcaaatgcc gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             20  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 541  
             
           
            20 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagca    120 
atgtcattgt ccggattgtt tgcgaacgcc gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             21  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 542  
             
           
            21 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttcctgca    120 
atgtcattgt ccggactttt tgccaacgca gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             22  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 548  
             
           
            22 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagca    120 
atgtcattgt ccggactctt tgcaaacgcc gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             23  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 549  
             
           
            23 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttcctgca    120 
atgtccttgt ccggactttt tgccaatgca gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             24  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 562  
             
           
            24 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagcc    120 
atgtcattgt ccggactttt tgccaacgca gtcctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             25  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 576  
             
           
            25 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagca    120 
atgtcattgt ccggactgtt tgcgaacgcg gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             26  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 579  
             
           
            26 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagca    120 
atgtcattgt ccggactttt tgccaacgcg gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             27  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 580  
             
           
            27 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagca    120 
atgtcattgt ccggactgtt tgccaacgcc gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             28  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 581  
             
           
            28 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagcc    120 
atgtccttgt ccggactttt tgccaacgca gtcctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             29  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 584  
             
           
            29 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccgtacaaa tcatagaggg tatttaatat gtttccagca    120 
atgtcattgt ccggactttt tgcgaatgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             30  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 491  
             
           
            30 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
catgtccttg tccggattgt ttgcgaatgc ggtcctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             31  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 529  
             
           
            31 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
aatgagcttg tccggattgt ttgcgaatgc ggtgctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             32  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 530  
             
           
            32 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttccagc    120 
catgtcattg tccggactct ttgcgaatgc ggtcctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             33  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 619  
             
           
            33 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
gatgagcttg tccggattgt ttgcgaatgc agtgctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             34  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 605  
             
           
            34 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttccagc    120 
aatgtccttg tccggactgt ttgcgaacgc agtcctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             35  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 606  
             
           
            35 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
aatgtccttg tccggattgt ttgcgaacgc ggtcctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             36  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 607  
             
           
            36 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
catgtccttg tccggactct ttgccaacgc tgtgctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             37  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 620  
             
           
            37 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
aatgagcttg tccggacttt ttgcgaatgc tgtactccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             38  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 631  
             
           
            38 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
catgagcttg tccggattgt ttgcaaacgc cgtcctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             39  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 632  
             
           
            39 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttccagc    120 
catgtcattg tccggattgt ttgcaaacgc tgtcctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             40  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 633  
             
           
            40 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttccagc    120 
catgtcattg tccggacttt ttgcgaacgc tgtgctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             41  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 635  
             
           
            41 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgtttcctgc    120 
catgtcattg tccggacttt ttgccaatgc ggtcctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             42  
             685  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 681  
             
           
            42 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gccagacact aaatagaggg tatttaatta tgttcccagc    120 
catgtcattg tccggacttt ttgccaacgc ggtgctccgg gctcagcacc tgcatcagct    180 
ggctgctgac accttcaaag agtttgagcg cacctacatc ccggagggac agagatactc    240 
catccagaac acccaggttg ccttctgctt ctctgaaacc atcccggccc ccacgggcaa    300 
gaatgaggcc cagcagaaat cagacttgga gctgcttcgc atctcactgc tcctcatcca    360 
gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg    420 
cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat    480 
gcgggagctg gaagatggca ccccccgggc tgggcagatc ctcaagcaga cctatgacaa    540 
atttgacaca aacatgcgca gtgacgacgc gctgctcaag aactacggtc tgctctcctg    600 
cttccggaag gacctgcata agacggagac gtacctgagg gtcatgaagt gccgccgctt    660 
cggggaggcc agctgcgcct tctag                                          685 
 
           
             43  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 483  
             
           
            43 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcctaaaaga gactaggagg agattagaat gttcccagca    120 
atgagtttgt ccggactgtt tgcgaacgcc gtgctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             44  
             684  
             DNA  
             Artificial Sequence  
             
               Somatotropin expression vector pXT 564  
             
           
            44 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcctaaaaga gactaggagg agattagaat gttcccagca    120 
atgtcattgt ccggactgtt tgccaatgcg gtcctccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctag                                           684 
 
           
             45  
             25  
             DNA  
             Artificial Sequence  
             
               Synthetic oligonucleotide  
             
           
            45 
cataacatca agaggatatg aaatt                                           25 
 
           
             46  
             25  
             DNA  
             Artificial Sequence  
             
               Synthetic oligonucleotide  
             
           
            46 
gtacaaatca tagagggtat ttaat                                           25 
 
           
             47  
             26  
             DNA  
             Artificial Sequence  
             
               Synthetic oligonucleotide  
             
           
            47 
agacactaaa tagagggtat ttaatt                                          26 
 
           
             48  
             25  
             DNA  
             Artificial Sequence  
             
               Synthetic oligonucleotide  
             
           
            48 
taaaagagac taggaggaga ttaga                                           25 
 
           
             49  
             983  
             DNA  
             Artificial Sequence  
             
               Synthetic construct for expression of bovine 
      somatotropin  
             
           
            49 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgagcttgt ccggactctt tgccaatgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gagatactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc cacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
tcgtggcttg ggcccctgca gttcctcagc agagtcttca ccaacagctt ggtgtttggc    420 
acctcggacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgggagctgg aagatggcac cccccgggct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccggaagg acctgcataa gacggagacg tacctgaggg tcatgaagtg ccgccgcttc    660 
ggggaggcca gctgcgcctt ctagaagctt aattctcact cattaggcac cccaggcttt    720 
acactttatg cttccggctc gtataatgtg tggaattgtg agcggataac aatttcacac    780 
aggaaacagc tgattgccct tcaccgcctg gcctccgttg agccatctgg atcggcagcg    840 
ttgtcttcat caaccggaac gagcatgccg gagagcagct cactcattag gcaccccagg    900 
ctttacactt tatgcttccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc    960 
acacaggaaa cagaattaag ctt                                            983 
 
           
             50  
             977  
             DNA  
             Artificial Sequence  
             
               Synthetic construct for expression of bovine 
      somatotropin  
             
           
            50 
gaattctact gtatgagcat acagttaagg gttgacaacc gatatttatt cacttaatat     60 
ataaatatca actgaggcgc gcccataaca tcaagaggat atgaaattat gtttccagcc    120 
atgagcttgt ccggactctt tgccaatgct gtactccggg ctcagcacct gcatcagctg    180 
gctgctgaca ccttcaaaga gtttgagcgc acctacatcc cggagggaca gcgttactcc    240 
atccagaaca cccaggttgc cttctgcttc tctgaaacca tcccggcccc gacgggcaag    300 
aatgaggccc agcagaaatc agacttggag ctgcttcgca tctcactgct cctcatccag    360 
agctggcttg ggccgctgca gttcctcagc cgtgtcttca ccaacagctt ggtgtttggc    420 
accagcgacc gtgtctatga gaagctgaag gacctggagg aaggcatcct ggccctgatg    480 
cgtgagctgg aagatggcac cccgcgtgct gggcagatcc tcaagcagac ctatgacaaa    540 
tttgacacaa acatgcgcag tgacgacgcg ctgctcaaga actacggtct gctctcctgc    600 
ttccgtaagg acctgcataa gacggagacg tacctgcgtg tcatgaagtg ccgccgcttc    660 
ggggaggcga gctgcgcctt ctaatagctc gagtctagac tcactcatta ggcaccccag    720 
gctttacact ttatgcttcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt    780 
cacacaggaa aaagctgatt gcccttcacc gcctggcctc cgttgagcca tctggatcgg    840 
cagcgttgtc ttcatcaacc ggaacgagca tgccggagag cagctcactc attaggcacc    900 
ccaggcttta cactttatgc ttccggctcg tataatgtgt ggaattgtga gcggataaca    960 
atttcacaca gaagctt                                                   977