Abstract:
The composition of the Nano carrier or Nano polymers composed of Hyaluronic Acid (15-25%) and fatty acids (50-70%) cross linked with ultra-Low molecular weight chitosan (15-25%) encapsulating isolated compounds from musk and their combinations for cosmetic use as anti-aging, anti-microbial, and fragrance. Additionally, these nano-encapsulated Musk bioactive compounds (MBC) at 0.1-10% (w/w MBC/Nano polymers) could be used in textile manufacturing (carpet/rugs and clothes) to provide anti-microbial and fragrance properties. Furthermore, Musk derived products are chemically conjugated to HA polymer, FA polymer, CH polymer, HA-CH co-polymer, FA-CH co-polymer or HA-CH-FA co-polymer to be incorporated into the fabric of the textile products (carpet/rugs or clothes).

Description:
RELATED APPLICATION 
       [0001]    The present invention claims priority to U.S. Provisional No. 61/936,919, filed on Feb. 7, 2014, which is incorporated herein by reference in its entirety. 
     
    
     FIELD OF THE INVENTION 
       [0002]    The present invention relates to a nanoformulation applicable to cosmetic and textile (carpet/rugs, and clothes) manufacturing for providing fragrance and antimicrobial properties in cosmetic and textile products. 
       BACKGROUND OF THE INVENTION 
       [0003]    Existing textile (carpet/rugs, and clothes) products might easily be a carrier of different microbes from the environment including bacteria, fungi and certain viruses along with bed smells. Additionally, having naturally driven anti-microbial with fragrance in cosmetic could be extremely valuable for infection prevention while it has appealing smell. 
         [0004]    Hence, there is a need for naturally driven anti-microbial with appealing musk fragrance to be impeded within the cosmetic products and to be impeded within the polymeric fibers of the different textile products. 
       SUMMARY OF THE INVENTION 
       [0005]    The present invention provides a composition, comprising: a nano-polymer comprising hyaluronic acid (HA polymer) and fatty acids (FA Polymer) cross linked with chitosan (CH) resulting in HA-CH, FA-CH or HA-CH-FA; and compounds encapsulated within the nano-polymer, said compounds derived from musk. Additionally, musk derived products are chemically conjugated to HA polymer, FA polymer, CH polymer, HA-CH co-polymer, FA-CH co-polymer or HA-CH-FA co-polymer to be incorporated into the fabric of the textile products (carpet/rugs or clothes). 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0006]      FIG. 1  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan (derived from mushroom) cross-linked nano-polymer, in accordance with embodiments of the present invention. 
           [0007]      FIG. 2  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan cross linked with Hyaluronic acid, in accordance with embodiments of the present invention. 
           [0008]      FIG. 3  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan cross linked with Musk derived fatty acids, in accordance with embodiments of the present invention. 
           [0009]      FIG. 4  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using Musk derived fatty acids. 
           [0010]      FIG. 5  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan cross linked with Musk derived fatty acids and alcohol, in accordance with embodiments of the present invention. 
       
    
    
       [0011]    In  FIG. 1-5 , Musk derived products such as M1 and/or M2 are chemically conjugated to HA polymer, FA polymer, CH polymer, HA-CH co-polymer, FA-CH co-polymer or HA-CH-FA co-polymer to be incorporated into the fabric of the textile products (carpet/rugs or clothes). 
       DETAILED DESCRIPTION OF THE INVENTION 
       [0012]    The present invention provides a nanoformulation applicable that may be used for cosmetic and textile manufacturing to providing fragrance and antimicrobial properties in cosmetic and textile products such as carpet, rugs or clothes. 
         [0013]    Hyaluronic acid (HA) is present in the extracellular matrix in the basal layer of the epidermis and it promotes keratin productions which affect skin hydration. HA also interacts with a cell-surface glycoprotein to support collagen synthesis and normal skin function. 
         [0014]    Fatty acids (FA) derived from different types of musk including white musk and others. FA examples—Tetradecanoic acid, other fatty acids isolated from Musk as shown in the detailed isolated FA. 
         [0015]    Chitosan (CH) is derived from mushroom and processed to produce low to ultra-low molecular weight for cross bridging with HA and FA. 
         [0016]    Musk derived products such as M1 and/or M2 are chemically conjugated to HA polymer, FA polymer, CH polymer, HA-CH co-polymer, FA-CH co-polymer or HA-CH-FA co-polymer to be incorporated into the fabric of the textile products (carpet/rugs or clothes). 
         [0000]    Encapsulation of Different Ingredients Isolated from Musk and Derivatives Include:
 
P-Anisaldehyde, P-hydroxyacetophenone, 3-hydroxy P-Anisaldehyde, and their combination for synergistic anti-microbial activity encapsulated into HA-FA-CH Nanoparticles having 100-300 nm and zeta potential of +5 to 20 my. Using Honey for wound dressing.
 
         [0017]    List all isolated aromatic compounds that are isolated and synthesized having anti-microbial activity to be encapsulated in the above Nano carrier (Tables and FIGS. below). 
         [0018]    Antimicrobial efficacy of 3-hydroxyl p-Anisaldehyde (C 8 H 8 O 3 ) and p-Hydroxy-acetophenone (C8H8O2) compounds: 
       Example 1 
     Materials and Methods 
       [0019]    (i) Musk: Natural Musk (animal Musk) used in this study was extracted from navel (deer) as a powder.
 
(ii) Test organisms:
 
         [0020]    Pathogenic Fungi:  Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Fusarium oxysporum  and  Candida albicans  were cultured on sabaroud dextrous agar media (Oxioid CM 41) at 25° C. 
         [0021]    Pathogenic bacteria:  Staphylococcus aureus  and  Pseudomonas aeroginosa  were cultured on Mueller Hinton media (Oxioid CM 41) at 37° C. 
       Example 2 
       [0022]    Extraction of non-alkaloid compounds from musk: 6 g of lipid free musk powder were placed into a soxhlet apparatus and extracted with 50 ml methanol for 8 hours at a temperature of 40-60° C. The filtered solution was then concentrated to dryness under vacuum at 45° C. The obtained dry extract was mixed with acidic water (PH=2). The supernatant was filtered through a 0.22-μm nylon membrane. The filtrate was then extracted by diethyl ether using separating funnel. The layer of diethyl ether was then separated and evaporated. The obtained gummy residue was then subjected to thin layer chromatography using petroleum ether and ethyl acetate for separating the active compounds. Two compounds were separated namely hydroxyl p-Anisaldehyde (C8H8O3) (0.011 g) (Rf=0.47) and p-Hydroxyacetophenone C 8 H 8 O 2 ) (0.009 g) (Rf=0.47). The two compounds were identified by GC mass and HPLC. 
       Example 3 
       [0023]    HPLC Analysis of separated compounds: The hydroxyl p-Anisaldehyde (C 8 H 8 O 3 ) and p-Hydroxyacetophenone were quantified by HPLC coupled with UV detection (HPLC-UV). HPLC analysis was performed with an HPLC instrument (Agilent 1100, USA) equipped with a quaternary solvent delivery system, a column oven, and an UV detector (Agilent 1100, VWD, USA). Twenty microliters of sample solution were injected into the column manually. Separation was achieved on an Hypersil ODS2 column (4.6 mm×250 mm, 5 μm) from Dalian Elite Analytical Instruments Co., Ltd. The column temperature was set to 25° C. and the detection wavelength was set at 280 nm. The mobile phase was 25% methanol with isocratic elution at a flow rate of 1.0 ml/min.  FIGS. 1 and 2  show a chromatogram for the simultaneous determination of the two compounds respectively 
       Example 4 
       [0024]    Biological activity: Antimicrobial activities of the 3-hydroxyl p-Anisaldehyde (C 8 H 8 O 3 ) and p-Hydroxy-acetophenone (C8H8O2) compounds were tested against the fungal species  Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Fusarium oxysporum  and  Candida albicans . Also both tested compounds were tested the bacterial species  Staphylococcus aureus  and  Pseudomonas aeroginosa.    
       Example 5 
       [0025]    Antifungal activities: The agar disc diffusion method was used to evaluate the antifungal activities of the tested compounds Haseneko{hacek over (g)}lu (1990). 50 ml of sabaroud dextrous agar media contained 1 ml from Suspension of fungi, then poured into sterile Petri dishes (9 cm in diameter) and left to solidify. Filter paper discs (6 mm in diameter) were soaked with 20 μl of the stock solutions (0.5 ml of the 3-hydroxyl p-Anisaldehyde (C 8 H 8 O 3 ) and p-Hydroxyacetophenone (C8H8O2) dissolved in Chloroform (CHCl 3 ) (100.0 μgml −1 )) and placed on the inoculated plates. The diameter of the inhibition zones were measured in millimeters after 4 days at 25±2° C. Table (1). 
       Example 6 
       [0026]    Antibacterial activities: Antibacterial activities of the tested compounds were employed by used the agar disc diffusion method Haseneko{hacek over (g)}lu (1990). Suspension of the tested bacteria (10 6  CFU/μl, O.D was measured calorimetry using spectrophotometer (Spectro, labomed, Inc.) at 620 mm) were spread on Mueller Hinton Agar (Oxioid). Each test solutions was prepared in CHCl 3  filter paper discs (6 mm in diameter) were soaked with 20 μl of the stock solutions and placed on the inoculated plates. The diameter of the inhibition zones were measured in millimeters after 24 h at 37° C. The results were confirmed using another method which was the agar well diffusion method would be employed for the determination of antibacterial and anti-fungal activities of 3-hydroxyl p-Anisaldehyde (C 8 H 8 O 3 ) and p-Hydroxyacetophenone (C 8 H 8 O 3 ) at and 0.5% (Collins, et at 1989). 
       Example 7 
       [0027]    Anti-Microbial Activities: The results showed that the 3-hydroxyl p-Anisaldehyde (C 8 H 8 O 3 ) and p-Hydroxyacetophenone (C 8 H 8 O 3 ) have the ability to inhibit the growth of tested microorganisms fungi and bacteria. Inhibition clear zones were observed around the filter paper discs (Table 1). From the results it was observed that the best inhibition of Pathogenic bacteria and fungi were obtained when using the two compounds together who use each one separately. 
         [0028]      FIG. 1  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan (derived from mushroom) cross linked nano-polymer, in accordance with embodiments of the present invention. 
         [0029]      FIG. 2  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan cross linked with Hyaluronic acid, in accordance with embodiments of the present invention. 
         [0030]      FIG. 3  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan cross linked with Musk derived fatty acids, in accordance with embodiments of the present invention. 
         [0031]      FIG. 4  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using Musk derived fatty acids. 
         [0032]      FIG. 5  depicts Nano-encapsulation of musk derived bioactive compounds (M1 and/or M2) with anti-microbial and fragrance characteristics using natural chitosan cross linked with Musk derived fatty acids and alcohol, in accordance with embodiments of the present invention. 
         [0033]      FIG. 1-5 , Musk derived products such as M1 and/or M2 are chemically conjugated to HA polymer, FA polymer, CH polymer, HA-CH co-polymer, FA-CH co-polymer or HA-CH-FA co-polymer to be incorporated into the fabric of the textile products (carpet/rugs or clothes). Table 1: Diameter of inhibition zone of 3-hydroxyl p-Anisaldehyde (C 8 H 8 O 3 ) and p-Hydroxy-acetophenone (C8H8O2) compounds against  Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Fusarium oxysporum, Candida albicans Staphylococcus aureus  and  Pseudomonas aeruginosa   
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                   
                 Diameter of inhibition 
               
               
                   
                 Test Organisms 
                 Zone (mm) 
               
               
                   
                   
               
             
             
               
                   
                 Control 
                 00.00 
               
               
                   
                 
                   Aspergillus niger 
                 
                 32.00 
               
               
                   
                 
                   Aspergillus fumigatus 
                 
                 27.50 
               
               
                   
                 
                   Aspergillus flavus 
                 
                 15.00 
               
               
                   
                 
                   Candida albicans 
                 
                 23.00 
               
               
                   
                 
                   Staphylococcus aureus 
                 
                 20.00 
               
               
                   
                 
                   Pseudomonas aeroginosa 
                 
                 18.00 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Bioactive Compounds Isolated from Musk Extracts 
               
             
          
           
               
                 Musk Extracts 
                 Structure 
                 Symbol 
               
               
                   
               
               
                 Vanillin 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M1 
               
               
                   
               
               
                 O-Benzyl-L-serine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M2 
               
               
                   
               
               
                 N-Cbz-L-tyrosine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M3 
               
               
                   
               
               
                 Benzyloxycarbonyl- L-arginine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M4 
               
               
                   
               
               
                 Benzoic acid, 4- hydroxyl-3- methoxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M5 
               
               
                   
               
               
                 P-Anisaldehyde, 3-hydroxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 *M6 
               
               
                   
               
               
                 Acetophenone,4′- hydroxyl- 3′, 5′- dimethoxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 *M7 
               
               
                   
               
               
                 Diisooctyl phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M8 
               
               
                   
               
               
                 Diethyl Phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M9 
               
               
                   
               
               
                 Musk ambrette Benzene, 1-[1,1- dimethylethyl]2- methoxy-4-methyl- 3,5-dinitro 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M10 
               
               
                   
               
               
                 Isobutyl phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M11 
               
               
                   
               
               
                 Diisooctyl phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 M12 
               
               
                   
               
               
                 Myristic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 MA 
               
               
                   
               
               
                 n-Caprylic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 CA 
               
               
                   
               
               
                 Oleic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 OA 
               
               
                   
               
               
                 Triethylene glycol methyl ether 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 TEGME 
               
               
                   
               
               
                 Alfol 14 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 Alfol 14 
               
               
                   
               
               
                 2-Methylhexadecan- 1-ol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 MHD 
               
               
                   
               
               
                 *M6 and *M7 have synergistic antimicrobial activity (Against Aspergillus Niger, Aspergillus fumigetus, Aspergillus flavus, Fusarium oxysporium) 
               
             
          
         
       
     
         [0000]    
       
                 
         
             
             
         
       
     
       Bioactive Compounds in Musk Extract 
       [0034]      
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Musk Extracts (I, II, III, IV) 
               
             
          
           
               
                 Extract “I” 
                 Extract “II” 
                 Extracts “III” 
                 Extract “IV” 
               
               
                   
               
               
                 Vanillin 
                 Dihydroergotamine 
                 [2S,3S]-[+]-2,3- 
                 Heptanol 
               
               
                   
                   
                 Butanediol 
               
               
                 O-Benzyl-L-serine 
                 N-Methyl-2-pyrrolidone 
                 1-Methoxy-2-propyl 
                 n-Caprylaldehyde 
               
               
                   
                   
                 acetate 
               
               
                 N-Cbz-L-tyrosine 
                 2-Methyl-4,5- 
                 n-Nonanal 
                 n-Nonanal 
               
               
                   
                 tetramethylene-5-ethyl-2- 
               
               
                   
                 oxazoline 
               
               
                 Benzyloxycarbonyl- 
                 Prolog [1,2-a]pyrazine- 
                 n-Doccosane 
                 n-Hepatadecane 
               
               
                 L-arginine 
                 1,4-dione, hexahydro-3- 
               
               
                   
                 [2-methylpropyl] 
               
               
                 Myristic acid 
                 Acetophenone, 4′- 
                 Hexadecanal cyclic 
                 Diethyl Phthalate 
               
               
                   
                 hydroxy-3′,5′-dimethoxy 
                 ethylene acetal 
               
               
                 Benzoic acid, 4- 
                 Phenol, o-ter-butyl 
                 3-Pentanol, 2,4- 
                 Fornesan 
               
               
                 hydroxyl -3- 
                   
                 dimethyl 
               
               
                 methoxy 
               
               
                 Benzoic acid 3, 
                 Uracil, 1,3,5-trimethyl 
                 Alfol 14 
                 n-Heptadecane 
               
               
                 hydroxy 
               
               
                 Benzoic acid 4, 
                 Phenol, 2,4- di-tert-butyl 
                 n-Nonylphenol 
                 Benzene, 1-[1,1- 
               
               
                 hydroxy 
                   
                   
                 dimethylethyl]2- 
               
               
                   
                   
                   
                 methoxy-4-methyl- 
               
               
                   
                   
                   
                 3,5-dinitro 
               
               
                 Ethanone, 1- [4- 
                 Acetophenone,4′- 
                 n-pentadecane 
                 Isobutyl phthalate 
               
               
                 hydroxy-3- 
                 hydroxyl- 3′,5′- 
               
               
                 methoxyphenyl] 
                 dimethoxy 
               
               
                 Ethanone, 1- [4- 
                   
                 Isobutyl phthalate 
                 n-Nonadecane 
               
               
                 hydroxyphenyl] 
               
               
                 P-Anisaldehyde, 3- 
                   
                 1,2- 
                 Oleic acid 
               
               
                 hydroxy 
                   
                 Benzenedicarboxylic 
               
               
                   
                   
                 acid, dibutyl ester 
               
               
                 Isobutyric acid 
                   
                 Eicosane 
                 n-Heneicosane 
               
               
                 n- Butyric acid 
                   
                 Heneicosane 
                 Diisooctyl phthalate 
               
               
                 [s]-[+]-3-Methyl-2- 
                   
                 n-Hexatriacontane 
               
               
                 butanol 
               
               
                 Butanoic acid, 3- 
                   
                 Hexanedioic acid, 
               
               
                 methyl 
                   
                 diisooctyl ester 
               
               
                 2- Methyl butanoic 
                   
                 2-Methylhexadecan-1- 
               
               
                 acid 
                   
                 ol 
               
               
                 Pentanoic acid 
                   
                 Diisooctyl phthalate 
               
               
                 Acetoin 
               
               
                 n-Hexanoic acid 
               
               
                 Benzylaxycarbonyl- 
               
               
                 L-arginine 
               
               
                 N-Cbz-L-tyrosine 
               
               
                 Benzyl alcohol 
               
               
                 Heptoic acid 
               
               
                 Benzeneethanol 
               
               
                 3-Methyl-3- 
               
               
                 heptanol 
               
               
                 n-Caprylic acid 
               
               
                 Retardex 
               
               
                 B-Hydroxyethyl 
               
               
                 phenyl ether 
               
               
                 Phenylacetic acid 
               
               
                 Phenol, o-tert-butyl 
               
               
                 O-benzyl-L-serine 
               
               
                 Phenylpropionic 
               
               
                 acid 
               
               
                   
               
             
          
         
       
     
       Bioactive Compounds in Musk Extracts 
       [0035]      
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Musk Extracts “I”: 
               
             
          
           
               
                 Extract “I” 
                 Structure 
               
               
                   
               
               
                 Vanillin 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 O-Benzyl-L-serine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 N-Cbz-L-tyrosine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Benzyloxycarbonyl- L-arginine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Myristic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Benzoic acid, 4- hydroxyl-3- methoxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Benzoic acid 3, hydroxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Benzoic acid 4, hydroxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Ethanone, 1-[4- hydroxy-3- methoxyphenyl] 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Ethanone, 1-[4- hydroxyphenyl] 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 P-Anisaldehyde, 3- hydroxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Isobutyric acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n- Butyric acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 [s]-[+]-3-Methyl-2- butanol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Butanoic acid, 3- methyl 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 2-Methyl butanoic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Pentanoic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Acetoin 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Hexanoic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Benzyl alcohol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Heptoic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Benzeneethanol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 3-Methyl-3- heptanol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Caprylic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Benzoic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 2-Phenoxy ethanol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Phenylacetic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Phenol, o-tert-butyl 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 O-benzyl-L-serine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Phenylpropionic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Musk Extracts “II”: 
               
             
          
           
               
                 Extract “II” 
                 Structure 
               
               
                   
               
               
                 Di- hydro- ergotamine 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 N-Methyl-2- pyrrolidone 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 2-Methyl-4,5- tetramethylene- 5-ethyl-2- oxazoline 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Pyrrolo [1,2-a] pyrazine- 1,4-dione, hexahydro-3-[2- methylpropyl] 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Phenol, 2[1,1- dimethylethyl] 2-tert- butylphenol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Uracil, 1,3,5- trimethyl 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Phenol, 2,4- di-tert- butyl 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Acetophenone, 4′-hydroxyl- 3′, 5′- dimethoxy 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 2-Piperidone 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Triethylene glycol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                 methyl ether 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Musk Extracts “III”: 
               
             
          
           
               
                 Extracts “III” 
                 Structure 
               
               
                   
               
               
                 [2S,3S]-[+]- 2,3- Butanediol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1-Methoxy- 2-propyl acetate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Nonanal 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Doccosane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Hexadecanal  cyclic ethylene acetal 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 3-Pentanol,  2,4- dimethyl 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Alfol 14 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Nonylphenol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-pentadecane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Isobutyl phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1,2- Benzene- dicarboxylic acid, dibutyl ester 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Eicosane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Heneicosane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Hexa- triacontane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Hexanedioic acid, diisooctyl ester 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 2-Methylhexa- decan-1-ol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Diisooctyl phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 Musk Extracts “IV”: 
               
             
          
           
               
                 Extract “IV” 
                 Structure 
               
               
                   
               
               
                 Heptanol 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Caprylaldehyde 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Nonanal 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Hepatadecane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Diethyl Phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Dodecane, 2,6,10- trimethyl 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Heptadecane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Musk ambrette Benzene, 1-[1,1- dimethylethyl]2- methoxy-4- methyl-3,5-dinitro 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Isobutyl phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Nonadecane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Oleic acid 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 n-Heneicosane 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Diisooctyl phthalate 
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
         
       
     
         [0036]    While particular embodiments of the present invention have been described herein for purposes of illustration, many modifications and changes will become apparent to those skilled in the art. Accordingly, the appended claims are intended to encompass all such modifications and changes as fall within the true spirit and scope of this invention.