Abstract:
Disclosed herein are inbibitors of the multicatalytic protease enzyme which are represented by the general formula: ##STR1## Constituent members and preferred constituent members are disclosed herein. Methodologies for making and using the disclosed compounds are also set forth herein.

Description:
FIELD OF THE INVENTION 
     This invention relates to inhibitors of multicatalytic protease (MCP), to compositions including such inhibitors and to methods for the use of MCP inhibitors to, for example, retard loss of muscle mass incident to various physiological states. 
     BACKGROUND OF THE INVENTION 
     Eukaryotic cells constantly degrade and replace cellular protein. This permits the cell to selectively and rapidly remove proteins and peptides having abnormal conformations, to exert control over metabolic pathways by adjusting levels of regulatory peptides, and to provide amino acids for energy when necessary, as in starvation. See Goldberg, A. L. &amp; St. John, A. C. Annu. Rev. Biochem. 45:747-803 (1976). The cellular mechanisms of mammals allow for multiple pathways for protein breakdown. Some of these pathways appear to require energy input in the form of adenosine triphosphate (&#34;ATP&#34;). See Goldberg, A. L. &amp; St. John, supra. 
     Multicatalytic protease (MCP, also referred to as &#34;multicatalytic proteinase,&#34; &#34;proteasome,&#34; &#34;multicatalytic proteinase complex,&#34; &#34;multicatalytic endopeptidase complex,&#34; &#34;20S proteasome&#34; and &#34;ingensin&#34;) is a large molecular weight (700kD) eukaryotic cytoplasmic proteinase complex which plays a role in at least two cellular pathways for the breakdown of protein to peptides and amino acids. See Orlowski, M. Biochemistry 29(45) 10289-10297 (1990). The complex has at least three different types of hydrolyric activities: (1) a trypsin-like activity wherein peptide bonds are cleaved at the carboxyl side of basic amino acids; (2) a chymotrypsin-like activity wherein peptide bonds are cleaved at the carboxyl side of hydrophobic amino acids; and (3) an activity wherein peptide bonds are cleaved at the carboxyl side of glutamic acid. See Livett, A. J. J. Biol. Chem. 264:21 12215-12219 (1989) and Orlowski, supra. 
     One route of protein hydrolysis which involves MCP also involves the polypeptide &#34;ubiquitin.&#34; Hershko, A. &amp; Crechanovh, A. Annu. Rev. Biochem. 51:335-364 (1982). This route, which requires MCP, ATP and ubiquitin, appears responsible for the degradation of highly abnormal proteins, certain short-lived normal proteins and the bulk of proteins in growing fibroblasts and maturing reticuloytes. See Driscoll, J. and Goldberg, A. L. PNAS 86:787-791 (1989). Proteins to be degraded by this pathway are covalently bound to ubiquitin via their lysine amino groups in an ATP-dependent manner. The ubiquitin-conjugated proteins are then degraded to small peptides by an ATP-dependent protease complexby the 26S proteasome, which contains MCP as its proteolytic core. Goldberg, A. L. &amp; Rock, K. L. Nature 357:375-379 (1992). 
     A second route of protein degradation which requires MCP and ATP, but which does not require ubiquitin, has also been described. See Driscoll, J. &amp; Goldberg, A. L., supra. In this process, MCP hydrolyzes proteins in an ATP-dependent manner. See Goldberg, A. L. &amp; Rock, K. L., supra. This process has been observed in skeletal muscle. See Driscoll &amp; Goldberg, supra. However, it has been suggested that in muscle, MCP functions synergistically with another protease, multipain, thus resulting in an accelerated breakdown of muscle protein. See Goldberg &amp; Rock, supra. 
     The relative activities of cellular protein synthetic and degradative pathways determine whether protein is accumulated or lost. The abnormal loss of protein mass is associated with several disease states such as muscular dystrophy, cardiac cachexia, emphysema, leprosy, malnutrition, osteomalacia, child acute leukemia, and cancer cachexia. Loss of muscle mass is also observed in aging, long term hospitalization or long term confinement to bed, and in chronic lower back pain. 
     With denervation or disuse, skeletal muscles undergo rapid atrophy which leads to a profound decrease in size, protein content and contractile strength. This atrophy is an important component of many neuromuscular diseases in humans. Enhancement of protein breakdown has been implicated as the primary cause of muscle wasting in denervation atrophy, Furono, K. et al. J. Biochem. 265/15:8550-8557 (1990). While the specific process or processes involved in protein hydrolysis in muscle has not been identified, evidence is available linking the involvement of MCP in the accelerated breakdown of muscle proteins. See, for example, Furono, supra, and PCT Published Application WO 92/20804 (publication date: Nov. 26, 1992). 
     MCP activity has been implicated in several disease states. For example, abnormally high expression of MCP in human leukemic cell lines has been reported. Kumatori, A. et al. PNAS 87:7071-7075 (1990). Autoantibodies against MCP in patients with systemic lupus erythematosus (&#34;SLE&#34;) have also been reported. Arribas, J. et al. J. Exp. Med. 173:423-427 (1990). 
     Agents which are capable of inhibiting the MCP complex are needed; such agents would provide a valuable tool for both those conducting research in the area of, for example, MCP activity, as well as those in the medical fields in order to, for example, control the deleterious effects of abnormal or aberrant MCP activity. The present invention is directed to these important ends. 
     SUMMARY OF THE INVENTION 
     The present invention is directed to novel multicatalytic protease (&#34;MCP&#34;) inhibitors. The subject invention also comprises methods for inhibition of MCP associated with certain disorders, including the treatment of muscle wasting disorders. In one aspect are provided compounds having the formula: ##STR2## Constituent members are defined infra, as well as preferred constituent members. 
     The compounds of the invention are useful in a variety of applications. For example, the compounds may be employed in research applications to further refine and develop in vitro and in vivo models for mechanistic understanding of the MCP pathway and for presentation of peptide antigens via the major histocompatibility complex class I (MHC I) pathway. 
     In a clinical setting, compositions comprising the claimed compounds can be used for inhibiting MCP activity, decreasing the loss of muscle mass, treating muscle wasting disorders, reducing superoxide dismutase degradation and treating disorders characterized by a reduction of superoxide dismutase activity. 
     Methodologies are also presented for making compounds of the invention. 
     These and other features of the compounds will be set forth in expanded form as the disclosure continues. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 shows the effect of embodiments of the disclosed MCP inhibitors on processing of electroporated OVA by M12.B6 cells. 
     FIG. 2 shows the effect of OVA concentration on the inhibition of processing by an embodiment of the invention. 
     FIG. 3 shows the effect of OVA concentration on the inhibition of processing by an embodiment of the invention. 
     FIG. 4 shows a physical map defining the SOD-1 gene and the location of the FALS mutations, restriction sites and the PCR primers. 
     FIG. 5 shows the quantitation of SOD-1 levels in transiently transfected 293 cells after incubation with 5 μM of embodiments of the MCP inhibitors. 
     FIG. 6 shows the dose response of various SOD-1 isoforms to an embodiment of the invention. 
     FIG. 7 shows that SOD-1 turnover is a function of MCP activity. 
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     This invention provides MCP inhibitors, compositions including these inhibitors and methods of using these inhibitors. The MCP inhibitors of the invention are represented by the formula: ##STR3## wherein: R 1  is --C.tbd.N, --C(O)OR 9 , phthalimido, --NH--SO 2  R 9 , or --NH--J; 
     R 2  is H, hydroxyl, alkyl having from one to ten carbons or cycloalkyl having from five to seven carbons; 
     R 3  is --(CH 2 ) m  --NH--C(═N--R 5 )--NH 2 , --R 6  --J, --R 6  --NO 2 , or --R 6  --CN; 
     R 4  is --CH(CH 2  --R 7 )--CH--R 8  ; 
     R 5  is --NO 2 , --CN, or --J; and 
     R 6  is --(CH 2 ) m  --NH--C(═NH)--NH--; 
     R 7  is alkyl having from one to eight carbons, said alkyl group being optionally substituted with one or more phenyl groups; and 
     R 8  is ═O, ═N--NHC(O)--NH 2 , ═N--OH, ═N--OCH 3 , ═N--O--CH 2  --C 6  H 5 , ═NNH--C(S)--NH 2  or ═N--NH--J; 
     R 9  is alkyl having from one to six carbons, said alkyl group being optionally substitued with one or more halogen atoms, aryl or heteroaryl groups; 
     J is a protecting group; 
     n is an integer from 5 to 10; and 
     m is an integer from 2 to 5. 
     In some preferred embodiments R 1  is --C.tbd.N, --C(O)OCH 3 , phthalimido or --NH--SO 2  CF 3 , and in other preferred embodiments R 2  is H or cyclopentyl. R 3  is preferably --(CH 2 ) 3  --NH--C (═N--R 5 )--NH 2 . 
     R 5  is preferably --NO 2 , --CN, --PMC, --MTR, --MTS, or Tos. R 7  is preferably --CH(CH 3 ) 2 , --(CH 2 ) 2  --CH 3  or C 6  H 5 , and R 8  is preferably ═O, ═N--OH, ═N--O--CH 2  --C 6  H 5 , ═NNH--C(O)--NH 2  or ═NNH--C(S)--NH 2 . 
     In some preferred embodiments R 1  is --C (O) OCH 3 , phthalimido or --NH--SO 2  CF 3  ; R 2  is cyclopentyl; R 3  is --(CH 2 ) 3  --NH--C(═N--NO 2 )--NH 2  ; R 7  is --CH(CH 3 ) 2  ; and R 8  is ═O. 
     In other preferred embodiments R 1  is --C.tbd.N; R 2  is cyclopentyl; R 3  is --(CH 2 ) 3  --NH--C(═N--NO 2 )--NH 2  or --(CH 2 ) 3  --NH--C(═N--J)--NH 2  ; R 7  is --CH(CH 3 ) 2  ; and R 8  is ═O. 
     In further preferred embodiments R 1  is --C.tbd.N; R 2  is cyclopentyl; R 3  is --(CH 2 ) 3  --NH--C(═N--NO 2 )--NH 2  or --(CH 2 ) 3  --NH--C(═N--J)--NH 2  ; R 7  is --CH(CH 3 ) 2  ; and R 8  is ═N--NHC (O)--NH 2 , ═N--OH, ═N--OCH 3 , or ═N--O-CH 2  --C 6  H 5 . 
     As used herein, the term &#34;alkyl&#34; is meant to include straight-chain, branched and cyclic hydrocarbons such as ethyl, isopropyl and cyclopentyl groups. Substituted alkyl groups are alkyl groups for which one or more hydrogen atoms have been replaced by halogen, other hydrocarbon groups (for example, a phenyl group), a heteroaryl group, or a group in which one or more carbon atoms are interrupted by oxygen atoms. Preferred alkyl groups have 1 to about 8 carbon atoms. As used herein, the term &#34;halogen&#34; has its usual meaning and includes fluorine, chlorine, bromine and iodine, with fluorine being a preferred halogen. The term &#34;Arg&#34; as used in the present invention has its normal meaning as the abbreviation for the amino acid &#34;arginine.&#34; 
     In some embodiments compounds of the invention contain protecting groups. As used herein, the phrase &#34;protecting groups&#34; is to be accorded a broad interpretation. Protecting groups are known per se as chemical functional groups that can be selectively appended to and removed from functionalities, such as hydroxyl groups, amino groups and carboxyl groups. These groups are present in a chemical compound to render such functionality inert to chemical reaction conditions to which the compound is exposed. Any of a variety of protecting groups may be employed with the present invention. One such protecting group is the phthalimido group. Other preferred protecting groups according to the invention have the following formulas: ##STR4## Further representative protecting groups suitable for practice in the invention may be found in Greene, T. W. and Wuts, P. G. M., &#34;Protecting Groups in Organic Synthesis&#34; 2d. Ed., Wiley &amp; Sons, 1991. 
     As previously indicated, MCP activity has been linked with a variety of disorders and diseases. Because compounds as disclosed herein are useful in inhibiting the activity of MCP, and because the usefulness of such compounds can be applied to both research and therapeutic settings for, methodologies for inhibiting the activity of MCP by contacting the MCP with a compound of the invention include providing the compound to a mammal, including a human, as a medicament or pharmaceutical agent. 
     As used herein, the term &#34;contacting&#34; means directly or indirectly causing placement together of moieties to be contacted, such that the moieties come into physical contact with each other. Contacting thus includes physical acts such as placing the moieties together in a container, or administering moieties to a patient. Thus, for example, administering a compound of the inventory to a human patient evidencing a disease or disorder associated with abnormal and/or aberrant activities of MCP which are associated with such disease or disorder, falls within the scope of the definition of term &#34;contacting.&#34; 
     In preferred embodiments, pharmaceutical compositions according to the invention are administered to patients suffering from a disorder, i.e., an abnormal physical condition, a disease or pathophysiological condition associated with abnormal and/or aberrant activities of MCP. The disorders for which the compositions of the invention are administered are preferably those which directly or indirectly produce a wasting (i.e., loss) of muscle mass, that is, a muscle wasting disorder. These include muscular dystrophies, cardiac cachexia, emphysema, leprosy, malnutrition, osteomalacia, child acute leukemia, AIDS cachexia and cancer cachexia. 
     In the context of the invention, &#34;administering&#34; means introduction of the pharmaceutical composition into a patient. Preferred methods of administration include intravenous, subcutaneous and intramuscular administration. Preferably, the compound will be administered as a pharmaceutical composition comprising the compound in combination with a pharmaceutically acceptable carrier, such as physiological saline. Other suitable carriers can be found in Remington&#39;s Pharmaceutical Sciences (Mack Pub. Co., Easton, Pa., 1980). 
     The concentrations of the compounds described herein in a pharmaceutical composition will vary depending upon a number of factors, including the dosage of the drug to be administered, the chemical characteristics (e.g., hydrophobicity) of the compounds employed, and the route of administration. In general terms, the compounds of this invention may be provided in an aqueous physiological buffer solution containing about 0.1 to 10% w/v compound for parenteral administration. Typical dose ranges are from about 1 μg/kg to about 1 g/kg of body weight per day; a preferred dose range is from about 0.01 mg/kg to 100 mg/kg of body weight per day. The preferred dosage of drug to be administered is likely to depend on such variables as the type and extent of progression of the disease or disorder, the overall health status of the particular patient, the relative biological efficacy of the compound selected, and formulation of the compound excipient, and its route of administration. As used herein the term &#34;patient&#34; denotes any type of vertabrate. Preferably, the patient is a human. 
     The muscular dystrophies are genetic diseases which are characterized byprogressive weakness and degeneration of muscle fibers without evidence of neural degeneration. In Duchenne muscular dystrophy (DMD) patients display an average of a 67% reduction in muscle mass, and in myotonic dystrophy, fractional muscle protein synthesis has been shown to be decreased by an average of 28%, without any corresponding decrease in non-muscle protein synthesis (possibly due to impaired end-organ response to anabolic hormones or substrates). Accelerated protein degradation has been demonstrated in the muscles of DMD patients. 
     Severe congestive heart failure (CHF) is characterizedby a &#34;cardiac cachexia,&#34; i.e., a muscle protein wasting of both the cardiac and skeletal muscles, with an average 19% body weight decrease. The cardiac cachexia is caused by an increased rate of myofibrillar protein breakdown. 
     Emphysema is a chronic obstructive pulmonary disease, definedby an enlargement of the air spaces distal to the terminal non-respiratory bronchioles, accompaniedby destructive changes of the alveolar walls. Clinical manifestations of reduced pulmonary functioning include coughing, wheezing, recurrent respiratory infections, edema, and functional impairment and shortened life-span. The efflux of tyrosine is increasedby 47% in emphysematous patients. Also, whole body leucine flux remains normal, whole-body leucine oxidation is increased, and whole-body protein synthesis is decreased. The result is a decrease in muscle protein synthesis, accompanied by a decrease in whole body protein turnover and skeletal muscle mass. This decrease becomes increasingly evident with disease progression and long term deterioration. 
     In diabetes mellitus, there is a generalized wasting of small muscle of the hands, which is due to chronic partial denervation (neuropathy). This is most evident and worsens with long term disease progression and severity. 
     Leprosy is associated with a muscular wasting which occurs between the metacarpals of the thumb and index finger. Severe malnutrition is characterized by, inter alia, severe muscle wasting. 
     Osteomalacia is a nutritional disorder caused by a deficiency of vitamin D and calcium. It is referred to as &#34;rickets&#34; in children and &#34;osteomalacia&#34; in adults. It is marked by a softening of the bones (due to impaired mineralization, with excess accumulation of osteoid), pain, tenderness, muscle wasting and weakness, anorexia, and overall weight loss. It can result from malnutrition, repeated pregnancies and lactation (exhausting or depleting vitamin D and calcium stores), and vitamin D resistance. 
     In childhood acute leukemia there is protein energy malnutrition which results in skeletal muscle wasting. Studies have shown that some children exhibit the muscle wasting even before diagnosis of the leukemia, with an average 27% decrease in muscle mass. There is also a simultaneous 33%-37% increase in adipose tissue, resulting in no net change in relative body weight and limb circumference. 
     Cancer cachexia is a complex syndrome which occurs with variable incidence in patients with solid tumors and hematological malignancies. Clinically, cancer cachexia is manifested as weight loss with massive depletion of both adipose tissue and lean muscle mass, and is one cause of death which results from cancer. Cancer cachexia patients have shorter survival times, and decreased response to chemotherapy. In addition to disorders which produce muscle wasting, other circumstances and conditions appear to be linked in some fashion with a decrease in muscle mass. Such afflictions include muscle wasting due to chronic back pain, advanced age, long term hospitalization due to illness or injury, alcoholism and corticosteroid therapy. 
     Studies have shown that in severe cases of chronic lower back pain, there is paraspinal muscle wasting. Decreasing paraspinal muscle wasting alleviates pain and improves function. 
     It is also believed that general weakness in old age is due to muscle wasting. As the body ages, an increasing proportion of skeletal muscle is replaced by fibrous tissue. The result is a significant reduction in muscle power, but only a marginal rejection in fat-free mass. 
     Studies have shown that in patients suffering injuries or chronic illnesses, and hospitalized for long periods of time, there is long-lasting unilateral muscle wasting, with an average 31% decrease in muscle mass. Studies have also shown that this can be corrected with intensive physiotherapy. However, it may be more effective for many patients to effect improvement with drug therapy. 
     In alcoholics there is wasting of the anterior tibial muscle. This proximal muscle damage is caused by neurogenic damage, namely, impaired glycolytic and phosphorylase enzyme activity. The damage becomes apparent and worsens the longer the duration of the alcohol abuse. Patients treated with corticosteroids experience loss of muscle mass. 
     The compounds of the invention can be used to alleviate the muscle mass loss resulting from the foregoing conditions, as well as others. Additionally, the MCP inhibitors of the invention are useful in veterinary and animal husbandry applications to, e.g., counter weight loss in animals, or to promote growth. 
     MCP has been implicated in the presentation of peptide antigens via the major histocompatibility complex class I (MHC I) pathway. See Goldberg and Rock, supra; see also Rock et al., Cell, 78:761-771 (1994) hereinafter &#34;Rock et al.&#34; Inhibitors of MCP therefore have utility as research reagents in studies where inhibition of the MHC I pathway is desired as well as in the alleviation of diseases and disorders which are associated with aberrant and/or abnormal MHC-I processing of antigens. Because the precise origin of most of the peptides presented on MHC-I molecules is still not clear and because evidence has recently accumulated that MCP may play a role in MHC-I presentation (see Rock et al. supra)), reagents such as the disclosed MCP inhibitors which block the protealytic processing of antigens for MHC-I presentation would be useful in resolving the importance of this pathway. 
     Surprisingly, it has also been found that MCP inhibitors of the invention are also useful in enhancing the activity of Cu/Zn superoxide dismutase-1 (&#34;SOD-1&#34;) enzyme. According, these compounds are useful in both research settings for the investigation of SOD-1 deficient systems and in the treatment of neurodegenerative or other disorders characterizedby a reduction in SOD-1 enzyme activity (i.e., wherein such a reduction has been implicated in the pathogenesis of the disorder). Such conditions include diseases involving oxidative stress such as Parkinson&#39;s disease, Alzheimers&#39;s disease, Huntington&#39;s disease, stroke, trauma, amyotrophic lateral sclerosis and ischemia. 
     SOD-1 is a homodimeric metalloenzyme that catalyzes the dismutation of the toxic superoxide anion O 2  •- to O 2  and H 2  O 2 . SOD-1 is a scavenger of free radicals and therefore acts as a first line defense in the detoxification of superoxide radicals, which are normal by-products of aerobic metabolism. SOD-1 occurs primarily in eukaryotes and is found in the cytoplasm of virtually all cell types. SOD-1 is an essential enzyme in the physiological response to oxygen toxicity and has been actively investigated as a therapeutic agent in pathological conditions related to oxidative stress. See Bannister et al., CRC Crit. Rev. Biochem. 22:111-180 (1987); Halliwell et al., Methods in Enzymol., 186:1-75 (1990); Greenwald, Free Rad. Biol. Med. 8:201-209 (1990). 
     Features that have prevented the use of SOD-1 as a therapeutic agent are its poor intracellular access when supplied exogenously, and its extremely short half-life in serum. Therefore, compounds that enhance the activity of intracellular SOD-1 would provide a significant advancement in SOD-1 therapy. 
     ALS is a progressive paralytic disorder caused by degeneration of large motor neurons of the spinal cord and brain. Approximately 5-10 % of ALS cases are familial (FALS) and are inherited as an autosomal dominant trait. Recently, sixteen different missense mutations have been identified in a subset of families with FALS and occur within the gene encoding SOD-1. See Rosen, D. R., et al., Science 261:1047-1051 (1993); Deng, H.-X., et al., Nature 362:59-62 (1993). These mutations lead to a decrease in SOD-1 activity in red blood cells and brain tissue, and have been shown to destabilize the SOD-1 protein resulting in increased turnover of the enzyme. See Bowling, A. C., et al., J. Neurochem. 61:2322-2325 (1993); Borchelt, D. R., et al., PNAS 91:8292-8296 (1994). Additionally, a transgenic-mouse model of ALS, based upon the implication of the connection between SOD-1 and ALS, has been described. Brown, R. H. 331/16 NEJM 1091 (1994). 
     We have discovered that our MCP inhibitors are potent positive effectors of SOD-1. A preferred MCP inhibitor, referred to herein as &#34;Compound 14,&#34; specifically reduces wild-type and mutant SOD-1 degradation in a dose-dependent manner (compound number designations are based upon the Example number which discloses the synthesis of the compound, e.g., the synthesis of Compound 14 is set forth in Example 14, infra). As used herein, reduction of SOD-1 degradation means retarding the rate at which the SOD-1 protein is catabolized. 
     The invention is further illustrated by way of the following examples. These examples are intended to elluciate the invention. The examples are not intended to limit the scope of the appended claims. 
     EXAMPLE 1 
     Isolation of Multicatalytic Protease from Human Tissues 
     Samples of human liver and brain obtained post-mortem were used for isolation and partial purification of MCP by ion-exchange chromatography, ammonium sulfate precipitation, and gel filtration (e.g., Driscoll and Goldberg, Proc. Natl. Acad. Sci. 86, 787-791 (1989); Yamamoto et al., Biochim, Biophys. Acta 882, 297-304 (1986). For either starting material, tissue was homogenized in 10 volumes of 20 mMTris-HCl (pH 7.5) containing 20% glycerol. Following centrifugation at 40,000× g for 30 minutes, proteolytic activity of the chymotrypsin-like component of MCP could be detected in the supernatant (see below). The supernatant was fractionated on a DEAE-Sepharose Fast Flow column equilibrated in homogenization buffer. For each liter of supernatant, 250 ml of resin was used. Following sample loading, the column was washed with ˜10 volumes of homogenization buffer, and proteins were eluted with a linear NaCl gradient of from 0 to 400 mM (2 liters for 250 ml resin). Fractions were assayed for MCP activity, and the active fractions were pooled and subjected to precipitation with (NH 4 ) 2  SO 4  at 80% saturation. Precipitated proteins were collected by centrifugation, resuspended in homogenization buffer, and loaded on a Sephacryl S300HR column (500 ml resin volume) that had been standardized using bovine serum albumin (68 kDa). A single peak of MCP activity was eluted with a molecular weight of ˜650 kDa. This preparation was free of other measurable proteolytic activities and maintained its MCP activity upon storage at 4° C. for &gt;6 months. This preparation was used for most of the experiments. Further fractionation of the preparation on an hydroxyapatite-Ultrogel column (Yamamoto et al., supra) yielded a more highly purified enzyme, which according to denaturing SDS-polyacrylamide gel electrophoresis was comprised of the expected &gt;10 subunits ranging in M r  from 20 to 35 kDa. 
     EXAMPLE 2 
     Assay for Chymotrypsin-Like and Trypsin-Like Activities of MCP 
     The procedure for MCP isolation described above generated an enzyme complex whose proteolytic activities were latent, but which could be activated by addition of low concentrations (0.02-0.05%) of SDS (Yamamoto et al., supra). The chymotrypsin-like activity was assayed according to the following procedure: in 96 well microtiter plates, human MCP was diluted 4 to 10-fold in homogenization buffer containing 0.04% SDS. A fluorogenic substrate, Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin (Suc-LLVY-AMC, purchased from Sigma or Bachem Philadelphia), was added to a final concentration of 100 μM from a stock solution of 10 mM in dimethylsulfoxide. Reaction volumes were 200 μL per well. After incubation for various periods of time at 37° C., the concentration of free AMC was determined on a Fluoroskan II spectrofluorimeter with an excitation filter of 390 nm and an emission filter of 460 nm. Protease activity was determined under conditions in which substrate hydrolysis increased linearly with time and the change in fluorescence was proportional to the concentration of free AMC. 
     The trypsin-like activity of human MCP was assayed as described above, except that reactions were carried out in Tris-glycerol buffer (pH 9.5) supplemented with 1 mM 2-mercaptoethanol, and the substrate was Benzyloxycarbonyl-Phe-Arg-AMC (100 μM). 
     EXAMPLE 3 
     Determination of IC 50  Values for MCP Inhibitors 
     IC 50  values, typically defined as the concentration of a compound (in this case, the disclosed MCP inhibitor) necessary to produce 50% inhibition of the enzyme&#39;s activity. IC 50  values are useful indicators of the activity of a compound for its designated use. Preferably, the inhibitors of the invention have IC 50  values of less than about 10 micromolar. 
     Inhibition of the chymotrypsin-like or trypsin-like activity of MCP was determined by incubating the enzyme with various concentrations of putative inhibitors for 15 minutes at 37° C. prior to the addition of substrate. Each experimental condition was evaluated in triplicate, and replicate experiments were performed for the inhibitors described herein. 
     EXAMPLE 4 
     Demonstration of Inhibition of Cellular Muscle Breakdown: Inhibition of Unweighting Atrophy in Juvenile Rats 
     The effect of several inhibitors on the unweighting atrophy of the soleus muscle in juvenile rats was determined. See Tischler, M. E. (1990) Metabolism 39/7:756-763 (hereinafter &#34;Tischler--1990&#34; for a general discussion of the procedure. 
     Juvenile female Sprague-Dawley rats (80-90 g) were tail-cast, hind limb suspended as in Jaspers, S. R. and Tischler, M. E., (1984) J. Appl. Physiol. 57:1472-1479. The animal&#39;s hind limbs were elevated above the floor of the cage with each animal housed individually. Animals had free access to food and water, and were weighed at the time of suspension and at time of termination. During the suspension period the animals were checked daily to ensure that their toes were not touching the floor of the cage, and that there was no swelling of the tail due to the cast. 
     A. Experimental Design--Part 1 
     Each experiment began with the suspension of 20 rats which were randomly divided into 4 groups of 5 animals each. Group A was suspended for just 2 days and provided baseline data to approximate the soleus muscle size in other animals suspended for longer times. Average body weights for the groups at the outset of the study were compared and used as a correction factor for body size differences. Group B was a second control group which had the soleus of one limb treated with an aqueos solution of mersalyl after two days of unweighting, to demonstrate the ability to slow muscle atrophy during unweighting, for each group of animals. Mersalyl has been previous studied and demonstrated to prevent atrophy in an in vivo model substantially as described in the protocol utilized herein. See Tischler--1990. At 2 days after unweighting commenced, an aqueos solution of mersalyl (200 nM; 4 μl/100 g initial body wt) was injected into one soleus, as described previously. The contralateral muscle was injected with a similar volume of 0.9% saline (&#34;Vehicle&#34;). The animals were maintained under Innovar-vet (10 μl /100 g body wt) tranquilization during the in situ injection procedure. After the injections, the animals were suspended for an additional 24 hours and the soleus was removed. Groups C and D for each experiment were used for testing each of two different embodiments of the disclosed compounds. Animals were treated as in Group B, except that 1 mM MCP inhibitor, contained in dimethysulfoxide (DMSO), was injected into the soleus of one leg and DMSO only into the contralateral soleus. Thus each experiment consisted of two control groups and the testing of MCP inhibitors of the invention. The completion of five such experiments with different pairs of inhibitors provided for an &#34;n&#34; value of 10 for testing each MCP inhibitor and with each tested in two different shipments of animals. 
     B. Processing of the Soleus Muscle--Part 1 
     After the animal was sacrificed, the soleus was excised, trimmed of fat and connective tissue, and carefully weighed. The muscle was then homogenized in 10% trichloroacetic acid (TCA) and the precipitated protein pelletedby centrifugation. The pellet was then washed once with 10% TCA and once with ethanol:ether (1:1). The final pellet was solubilized in 4 ml of 1N sodium hydroxide. The sample was then analyzed for protein content by the biuret procedure, using albumin as a standard. 
     C. Data analysis--Part 1 
     The effect of MCP inhibitors on total muscle protein content was examined primarily by paired comparison with the untreated contralateral muscle. The ratio of contents was calculated and then analyzed statistically by analysis of variance (&#34;ANOVA&#34;). The left leg was always the treated leg so that the protein content ratios could be compared to the non-treated control animals as well. In this way a significant difference can be shown by comparing the protein content of the two legs, as well as the relative effectiveness of the tested MCP inhibitors. A paired student test was also performed for the effect of each separate treatment. The non-treated control data also provided an estimate of protein content of day 2. This allowed for approximation of the protein changes over the 24 hours of treatment for each of the Groups B, C, and D. 
     D. Experimental Design--Part 2 
     Each experiment consisted of 10 animals with groups of 5 animals being tested with one of the inhibitors for its effect on protein synthesis. Control animals were not needed for this aspect of the study as the contralateral DMSO-treated muscle served as the paired control for the inhibitor-treated muscle. Each group was injected as described for Groups C and D in part 1. Twenty-four hours after the in situ treatment the fractional rate of protein synthesis was analyzed in both soleus muscles. Each muscle was injected with a 0.9% saline solution (3.5 μl/100 g final body wt) containing  3  H-phenylalanine (50 mM; 1 μCi/μl). Fifteen minutes later the middle two-thirds of the muscle was excised and the muscle was processed as described below. 
     E. Processing of the Soleus Muscle--Part 2 
     The muscle was first washed for 10 minutes in 0.84% saline containing 0.5 mM cycloheximide, to terminate protein synthesis, and 20 mM cycloleucine, to trap phenylalanine in the cell. The muscle was then homogenized in 2.5 ml of ice-cold 2% perchloric acid. The precipitated protein was pelleted by centrifugation. One aliquot of the supernatant was taken for liquid scintillation counting and another aliquot was processed for conversion of phenylalanine to phenethylamine to determine the soluble phenylalanine concentration fluorometrically. See Garlick, P. J. et al., Biochem. J., 192:719-723 (1980) and Munoz, K. M. et al., 1993 Metabolism, in press. These values provide the intracellular specific activity. The specific activity of phenylalanine in the muscle protein was determined after hydrolyzing the protein by heating in 6N HCl. The amino acids released were solubilized in buffer. One aliquot was taken for scintillation counting and another for analysis of phenylalanine as for the supernatant fraction. The fractional rate of protein synthesis was calculated as: protein specific activity/intracellular specific activity×time. 
     F. Data Analysis--Part 2 
     Analyses of protein synthesis were on a paired basis for each MCP inhibitor. Student paired t test comparisons of the contralateral muscles determined whether there was any effect of the inhibitor on protein synthesis. Protein breakdown can be calculated approximately as the fractional rate of protein synthesis (from part 2) plus the fractional rate of protein accretion (from part 1), where protein loss yields a negative value for protein accretion. 
     Qualitatively, the ability of MCP inhibitors to slow protein loss without affecting protein synthesis indicates a slowing of protein degradation. 
     G. Results 
     Table 1 shows the lack of effect of the MCP inhibitors on control muscle. 
     Table 2 shows the change in protein content during the third day of unweighting. 
     Table 3 shows the effect of the MCP inhibitors on unweighted muscle protein. 
     Table 4 shows the effect of the MCP inhibitors on unweighted muscle synthesis. 
     In the Tables, the compound number refers to the Example number where the synthesis route for the compound is set forth. 
     
                       TABLE 1______________________________________Lack of Effect of MCP Inhibitors on Control Muscle        Protein (mg/muscle)Treatment      Vehicle  Treatment______________________________________DMSO           5.9      6.7Compound 34    6.1      6.3Compound 14    6.0      6.1Compound 20    5.7      5.9______________________________________ 
    
     
                       TABLE 2______________________________________Change in Protein Content During Day 3 of Unweighting   Protein (mg/muscle)                 EffectTreatment   Start  End        (%)    P______________________________________Saline      6.5    5.7        -12    &lt;0.001DMSO        6.5    5.9        -10    &lt;0.001Mersalyl    6.4    6.6         +3    &lt;0.05Compound 34 6.3    6.6         +5    &lt;0.05Compound 14 6.8    6.4         0     &gt;0.1Compound 20 6.5    6.2         -4    &lt;0.07*Compound 16 6.3    6.0         -5    &lt;0.07*______________________________________ *marginally significant values 
    
     
                       TABLE 3______________________________________Effect of MCP Inhibitors on Unweighted Muscle Protein   Protein (mg/muscle)                  EffectTreatment Vehicle   Treatment  (%)    P______________________________________Mersalyl  5.7       6.6        14     &lt;0.001Compound 34     5.9       6.6        12     &lt;0.001Compound 14     6.0       6.4        7      &lt;0.06*Compound 20     5.9       6.2        5      &lt;0.05Compound 16     5.9       6.0        0      &gt;0.1______________________________________ *marginally significant values 
    
     
                       TABLE 4______________________________________Lack of Effect of MCP Inhibitors onUnweighted Muscle Synthesis        Protein (mg/muscle)Treatment      Vehicle  MCP Inhibitor______________________________________Compound 34    14.5     13.3Compound 14    13.1     13.8Compound 20    14.1     14.6______________________________________ 
    
     EXAMPLE 5 
     Demonstration of MCP Inhibition Using MHC-I Processing 
     Compounds of the invention were assayed for the ability to inhibit the processing of exogenous OVA antigert by the class I major histocompatibility pathway (MHC-I). The MCP inhibitors were applied to a functional antigen processing system that allows inclusion of the inhibitor within the antigen processing cell during exposure to antigen, followed by fixation of the processing cell. T cell hybridomas are then added to the processing cells in the absence of inhibitor to determine the expression of peptide-MHC-I complexes, which reflects antigen processing prior to fixation. 
     A. Cell Culture 
     Cells were grown at 37° C. in a humidified atmosphere maintained at 5% CO 2  in a standard medium: DMEM (Gibco, St. Louis, Mo.) with 10% fetal calf serum (Hyclone, Logan UT), 5×10 -5  M 2-mercaptoethanol, antibiotics and the following supplements: L-arginine HCL (116 mg/L), L-asparagine (36 mg/L), NaHCO 3  (2 g/L), sodium pyruvate (1 mM). See also Harding, C. V., (1992) Eur. J. Immunol. 22:1865-1869. M12.B6 cells (generous gift of Osami Kanagawa, Washington University, ST. Louis, Mo.) were used for antigen presentation; they were createdby fusing M12.C3 murine B lymphoma cells with LPS-stimulated splenocytes (source of B lymphoblasts) from a C57BL/6 mouse. Accordingly, they express the H-2 b  antigens. DOWB T hybridoma cells are specific for the OVA (323-339) peptide bound to either I-A b  or I-A d . See Yewdell et al. (1988), Science 1988 239:637. B3.1 T hybridoma cells respond to OVA (258-276)-K b . 
     B. Electroporation and Antigen Processing Studies 
     To be processed and presented by the MHC-I pathway, the exogenous antigen (ovalbumin; OVA) must penetrate into the cytosol of the processing cells. OVA was introduced into the cytosol of processing cells (M12.B6 cells) by means of electroporation. 
     Electroporation was performed with a Gibco BEL Electroporator using 0.4 cm gap cuvette chambers. Capacitance was 800 μF and voltage was 50-300 V. Electroporation was performed in serum-free RPMI or PMEM (Gibco) with 1.5×10 7  M12.B6 cells/ml (0.5×10 7  -4.0×10 7  range) in the presence of OVA at 4° C. The cells were immediately placed on ice. Various inhibitors were then added and the cells were transferred to 18° C. or 37° C. Finally the cells were lightly fixed for about 10 minutes with 1% paraformaldehyde and washed extensively at 37° C., preventing further processing. The extent of processing, (i.e., the level of specific peptide-MHC complexes expressed) was determinedby the ability of the M12.86 cells to stimulate IL-2 secretion by B3.1 or DOBW T hybridoma cells. T cells (10 5 ) were plated with the M12.B6 cells (2×10 5  if fixed, 5×10 4  if viable) in 0.2 ml for 18-24 hours at 37° C. in a 10 2  chamber. Both T hybridomas respond to antigen stimulation by the secretion of IL-2 (assayed in the supernatants by IL-2-dependent CTLL cell proliferation and [H 3  ] thymidine incorporation. See Allen et al., J. Immunol. 132:1077. 
     Results of these studies are shown in FIGS. 1-3. FIG. 1 shows the effects of Compounds 14 and 16 on the processing of electroporated OVA by M12.B6 cells. FIGS. 2 and 3 show the effect of Compounds 14 and 20 on MHC-I OVA processing. Both compounds effectively inhibit OVA processing. These data provide additional support for MCP inhibitory effect of the compounds of the invention. It is accepted that the classical MHC-I pathway involves antigen degradation by proteosomes. See Rock et al., supra. Thus, the compounds can be utilized in further understanding the importance of the protealytic procesing of antigens. 
     EXAMPLE 6 
     A. Reduction of Cu/Zn Superoxide Dismutase (SOD-1) Degradation 
     I. Part 1--Cloning and Mutagenesis 
     A mouse SOD-1 cDNA clone was obtained from J. Mahaffey (North Carolina State University, Raleigh, N.C.). This clone was modified by polymerase chain reaction (PCR) methodologies to incorporate 1) a Kozak translation initiation consensus signal (i.e., 5&#39;-GCCGCCACC-3&#39;) directly upstream of the ATG start codon, 2) a Hind III restriction site 5&#39; of this consensus signal and 3) an Xho I restriction site at the 3&#39; terminus of the cDNA. The oligonucleotide primers used for the PCR procedures were: 5&#39; primer (EH87) = 5&#39;-TCGATCGAAGCTTGCCGCCACCATGGCGATGAAAGC-3&#39; (SEQ ID NO:1), 3&#39; primer (EH88) = 5&#39;-AGCTAGCCTCGAGCAGATTACAGTTTAATG-3&#39; (SEQ ID NO:2). The resulting PCR product was digested with the restriction enzymes Hind III and Xho I and cloned into the Hind III + Xho I digested vector pBluescript II SK+ (Stratagene Cloning Systems, La Jolla, Calif.) to yield pSK-HX2. 
     The FALS point mutations at amino acid 4 (Ala 4  →Val) (GCG→GTG) and amino acid 113 (Ile  113  →Thr) (ATT→ACT) were introduced into the SOD-1 cDNA clone pSK-HX2 using the overlap extension PCR mutagenesis procedure described by Ho et al., Gene 77:51-59 (1989). The Ile  113  →Thr mutant was constructed as follows: the 5&#39; PCR fragment was generated using the 5&#39; primer EH78; consisting of the nucleotide sequence 5&#39;-TTAATCCTCACTCTAAGAAAC-3&#39; (SEQ ID NO:3) (nucleotides 193 to 213 of SOD-1 cDNA where #1 is the A of the ATG start codon) and the 3&#39; primer EH84; consisting of the nucleotide sequence 5&#39;-TTGTACGGCCAGTGATGGAATGCT-3&#39; (SEQ ID NO:4) (nucleotides 351 to 328), the 3&#39; PCR fragment was constructed using the 5&#39; primer EH83; consisting of the nucleotide sequence 5&#39;-AGCATTCCATCACTGGCCGTACAA-3&#39; (SEQ ID NO:5) (nucleotides 328 to 351) and the 3&#39; primer EH42; consisting of the nucleotide sequence 5&#39;-TAATACGACTCACTATAGGG-3&#39; (SEQ ID NO:6) (nucleotides 626 to 645 of pBluescript II SK+). Fifty nanograms of both 5&#39; and 3&#39; PCR fragments were combined in the second step PCR reaction and amplified using primers EH78 and EH42. This PCR product was digested with Bal I and Xho I and cloned into Bal I +Xho I digested pSK-HX2 thus replacing the native 255 bp Bal I/Xho I fragment with the FALS mutant fragment (clone pSK-113). 
     The Ala  4  →Val mutant was constructed as follows: the 5&#39; PCR fragment was generated using the 5&#39; primer EH105; consisting of the nucleotide sequence 5&#39;-GCACACCACTTTCATCGCCATGGTGGCGGCAAGCTTCGATC-3&#39; (SEQ ID NO:7) (nucleotides +21 to -20) and the 3&#39; primer EH41; consisting of the nucleotide sequence 5&#39;-ATTAACCCTCACTAAAGGGA-3&#39; (SEQ ID NO:8) (nucleotides 792 to 773 of pBluescript II SK+). The 3&#39; PCR fragment was generated using the 5&#39; primer EH104, consisting of the nucleotide sequence 5&#39;-GATCGAAGCTTGCCGCCACCATGGCGATGAAAGTGGTGTGC-3&#39; (SEQ ID NO:9) (nucleotides -20 to +21) and the 3&#39; primer EH103; consisting of the nucleotide sequence 5&#39;-CTTCAGTTAATCCTGTAA-3&#39; (SEQ ID NO:10) (nucleotides 123 to 106). As above, 50 ng of both 5&#39; and 3&#39; PCR fragments were combined in the second step PCR reaction and amplified using primers EH41 and EH103. This PCR product was digested with Hind III and Rsr II and cloned into Hind III+ Rsr II digested pSK-HX2 thus replacing the native 51 bp Hind III/Rsr II fragment with the FALS mutant fragment (clone pSK-A4V). All PCR products described above were sequenced using the Sequenase method (US Biochemical, Cleveland, Ohio) to confirm the presence of the mutations and the absence of any PCR errors. An illustration of the SOD-1 gene defining the locations of the mutations, primers and restriction sites is shown in FIG. 4. 
     The SOD-1 cDNA expression vectors were constructed by digesting the wild-type (pSK-HX2) and mutant constructs (pSK-113 and pSK-A4V) with Hind III and Xho I. Each of the three resulting 546 bp fragments containing the SOD-1 cDNA sequences were separately cloned into Hind III+Xho I digested pcDNA I/Neo (Invitrogen Corp., San Diego, Calif.) to yield, respectively, pCMV-HX5 (wild-type SOD), pCMV-113 (Ile  113  →Thr), and pCMV-A4V (Ala 4  →Val). 
     II. Part 2. 
     Initial experiments were conducted to determine whether the reduced stability of the FALS mutant SOD-1 proteins was due to MCP-mediated proteolytic degradation. The human kidney cell line designated 293 (human 293 cells or 293 cells) was obtained from the American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville, Md., 20852 (ATCC #CRL 1573) and grown in Dulbecco&#39;s modified Eagle&#39;s medium with 4.6 g/L glucose, 10% heat-inactivated horse serum (Gibco/Life Technologies Inc. Gaithersburg, Md.). The human 293 cells were maintained at 37° C. in an atmosphere of 5% CO2. The 293 cells were transiently transfected using the calcium phosphate method (Chen, C. et al., Biotechniques 6:632 (1988). The pCMV-113 SOD-1 expression vector was introduced into 293 cells and 24 hours later, the cells were incubated with either Compound 34, Compound 14, or Compound 24, all at a concentration of 5 μM, for a duration of 24 hours. The known MCP and other protease inhibitory compounds were solubilized in dimethyl sulfoxide (DMSO). Other 293 cell cultures were incubated with an equal volume of DMSO or left in medium only as negative controls. 
     Approximately 48 hours prior to receiving test compounds, 5×10 5  293 cells were seeded in 36 mm dishes and maintained at 37° C. in an atmosphere of 5% CO 2 . Cells were incubated with MCP inhibitors (compound 34, 14 or 24), or with DMSO as a negative control, for a duration of 24 hours. Cell lysates were preparedby lysing the cells in approximately 75 μl of phosphate-buffered saline (PBS) by freeze/thaw cycling. Protein concentrations of the cell lysates were determined using the BCA method (Pierce, Rockville, Ill.) and 2 to 2.5 μg of each sample was electrophoresed on a 4-20% polyacrylamide gel (Novex, San Diego, Calif.) using a Tris/glycine/SDS (25 mM Tris/192 mM glycine/0.1%SDS) buffer system. Proteins were transferred to nitrocellulose filters by electroelution and filters were blocked by incubation in blotto solution (5% dry milk in 25 mM Tris-buffered saline (1x TBS)) for 30 minutes. Filters were transferred to primary antibody solution (1:10,000 dilution in blotto solution) and incubated for 2-18 hours. The primary antibody used in these studies was polyclonal rabbit antiserum raised against purified mouse SOD-1 produced in E. coli (Hazelton Research Products, Denver, Pa.). The filters were washed three times for 5 minutes each in 1x TBS and incubated in secondary antibody solution (1:2,000 dilution in blotto solution) for two hours. The secondary antibody was a goat anti-rabbit IgG conjugated to alkaline phosphatase (Bio-Rad, Richmond, Calif.). Filters were washed three times for 5 minutes each in 1x TBS and stained for alkaline phosphatase activity by incubation for 5-60 minutes in a commercially available alkaline phosphatase detection reagent (Bio-Rad, Richmond, Calif.). Stained bands corresponding to SOD-1 protein were quantitated using a DocuGel V image analysis system and RFLPscan software (Scanalytics, Billerica, Mass.). Levels of SOD-1 are expressed as units of induction relative to the negative control (i.e., units of experimental divided by units of control). Immunoblot analysis of cell lysates revealed that SOD-1 levels were modestly elevated compared to those of controls in each of the three samples incubated in the presence of MCP inhibitors (FIG. 5). These results show that inhibition of the MCP by the compounds of the invention leads to increased accumulation of SOD-1 protein in the Ile  113  →Thr mutant and thereby implicates MCP as being responsible for the reduced levels of SOD-1 in the cells containing the FALS mutation. 
     III. Part 3 
     To further show that MCP activity is responsible for SOD-1 turnover, studies were conducted on cell lines stably producing wild-type mouse SOD-1 or the FALS mutant proteins, i.e., Val in place of Ala 4  and Thr in place of Ile 113 . Cell lines stably expressing mouse native SOD-1 and the two FALS mutant SOD-1 proteins mentioned above were derived by calcium phosphate transfection (Chen, C. et al., Biotechniques 6:632 (1988)) of 293 cells with SOD-1 cDNA expression constructs (described above) followed by selection for neomycin resistance by growth in the presence of 1 mg/ml G418 (Geneticin™, Gibco/Life Technologies, Inc., Gaithersburg, Md.). Cells were maintained in G418 selection for three weeks at which time drug selection was removed and the G418-resistant colonies of each transformed cell type were pooled for further growth. Compound 14 was utilized for use in these studies. 
     Cell lines expressing the indicated SOD-1 isoforms were incubated with various concentrations of compound 14 for 24 hours at which time the SOD-1 levels were measured by densitometric scanning of immunoblots. Levels of SOD-1 are expressed as units of induction relative to the samples from negative controls (as above) and each data point is the average of three experiments. Dose response analysis of Compound 14 indicated that incubation of the transformed cells with this MCP inhibitor led to significant accumulations of SOD-1 protein levels with maximal accumulations occurring in those cells incubated with Compound 14 at a concentration of approximately 20 μM (FIG. 6). Moreover, the magnitude of SOD-1 accumulation correlates with the estimated half-lives of the various SOD proteins; wild-type SOD-1 being the most stable and Ala 4  →Val the least stable (Borchelt D.R. et al., Proc. Natl. Acad. Sci. 91:8292-82-96 (1994)). These data are consistent with the hypothesis that the FALS mutations destabilize the SOD-1 proteins, possibly due to misfolding, and as a result of structural alterations resulting from the mutations target these proteins for degradation by MCP. These results also demonstrate that Compound 14 has a statistically significant effect on turnover of the wild-type SOD-1 protein further supporting the role of the MCP in SOD-1 degradation. 
     IV. Part 4 
     To demonstrate the specificity of MCP activity in SOD-1 turnover (and rule out the possible involvement of other major proteolytic pathways such as the calcium-activated protease calpain and the lysosomal proteases), experiments were performed in which two transformed cell lines (described above, one producing wild-type protein and the other SOD-1 protein, with the Ala 4  →Val mutation), were incubated with various protease inhibitors each of which is specific for a unique proteolytic activity: Compound 14, which inhibits MCP; &#34;Calpeptin&#34; (Novabiochem USA, La Jolla, Calif., cat no. 03-34-0051; CBZ-Leu-Nle-aldehyde, BBRC (1988) 153:1201), a cell penetrating &#34;calpain&#34; inhibitor (calpain is a cysteine protease); and DK-3 (Enzyme Systems Products, Livermore, CA CBZ-Phe-Ala-Chn 2 ) inhibits lysosomal protease activity. In addition, cells were incubated with Compound 16 which is a less potent MCP inhibitor compared to a particularly preferred compound of the invention, i.e. Compound 14. Cell lines expressing the indicated SOD-1 isoforms were incubated for 24 hours with the following protease inhibitors: Compound 14, 20 μM; Calpeptin, 10 μM; DK-3, 5 μM; Compound 16, 20 μM; or with DMSO as a negative control. After the 24 hours of incubation with the inhibitors, SOD-1 levels were measured by densitometric scanning of immunoblots. Levels of SOD-1 are expressed as units of induction relative to untreated samples, as above. Each data point is the average of results from three experiments. 
     Results are presented in FIG. 7. It can be seen that the turnover of Ala 4  →Val mutant SOD-1 is a function of MCP activity and only the specific inhibition of MCP by the preferred Compound 14 leads to a significant (three-fold) increase in the accumulation of SOD-1 protein. Incubation with the inhibitors of calpain or lysosomal proteases had no appreciable effect upon SOD-1 turnover. Further, Compound 14 treatment resulted in a slight increase in wild-type SOD-1 accumulation, a finding also observed previously in the dose response studies. Taken together, these studies demonstrate that MCP activity is critical for SOD-1 turnover in the transformed 293 cell lines, and that inhibition of MCP by Compound 14 leads to an elevation of SOD-1 protein within the cell. 
     Synthesis of Inhibitors 
     The following provides exemplary synthesis routes for the preparation of compounds of the present invention. Other synthesis protocols, as well as modifications of the following synthesis schemes will be readily apparent to those skilled in the art once armed with the present disclosure. 
     EXAMPLE 7 
     Method A: Mixed Anhydride Method: ##STR5## X=Fluorenylmethyloxycarbonyl (Fmoc)--or t-butyloxycarbonyl (Boc)- group; Y=Acetal or other protecting group for the amino aldehyde 
     AA 1  =Amino acid 
     AA 2  =Amino aldehyde 
     N-methylmorpholine (NMM) (1 eq. ) was added to a stirred solution of the protected amino acid in tetrahydrofuran (THF). The mixture was cooled to -15° C., treated with isobutyl chloroformate (IBCF) (1.1 eq.), and allowed to react for 10 min. Subsequently, the amino aldehyde component in the form of the free base was added, followedby NMM (1.1 eq., 2.2 eq. if acid salt). Stirring proceeded for 30 min at -15° C., and then at room temperature for 3-4 h. The reaction mixture was dissolved in 150-200 mL of ethyl acetate (EtOAc). The resulting solution was successively washed with water, 5% sodium hydrogen carbonate (NaHCO 3 ), 2% aqueous citric acid and, finally, water. The organic layer was dried over anhydrous sodium sulfate (Na 2  SO 4 ) or magnesium sulfate (MgSO 4 ), the solvent was removed under reduced pressure, and the product thus obtained was triturated with petroleum ether. The solid peptide thus obtained was filtered off, dried and characterized. 
     EXAMPLE 8 
     Method B: Fmoc-Group Deprotection Procedure 
     Scheme: 
     
         Fmoc--HN--AA.sub.1 --AA.sub.2 --Y→H.sub.2 N--AA.sub.1 --AA.sub.2 --Y 
    
     The Fmoc-protected peptide (0.2 to 4 mmol) in a mixture of 30% dimethylformamide (DMF) in ethyl acetate (EtOAc) was treated with a 50-60 fold excess of diethylamine (DEA) for 2 h at room temperature. The solvent was evaporated at reduced pressure at 30° C., and petroleum ether was added to the residue. When a precipitate formed it was filtered off and dried. In other cases, the resulting gum was repeatedly triturated with petroleum ether, and the gum was stored under vacuum. 
     EXAMPLE 9 
     Method C: Capping Group or Amino Acid Addition to the Peptide 
     Scheme: 
     
         R--COOH+H.sub.2 N--(AA).sub.n --COOR&#39;→R--CO--HN--(AA).sub.n --COOR&#39; 
    
     5 The capping group (as a free carboxylic acid) or protected amino acid (1 eq.), benzotriazol-1-yloxy-tris- (dimethyamino)-phosphonium hexafluorophosphate (BOP) (1.1 eq.) and 1-hydroxybenzotriazole (HOBt) (1 eq.) were dissolved in 5 mL of DMF followed by NMM (2.2 eq.). After 5 min, the deprotected basic component of the peptide or the carboxyl group protected amino acid was added, the pH was adjusted to 8 and the mixture was stirred for 3-4 h. It was then diluted with 150-300 mL of EtOAc and extracted consecutively with water, 2% NaHCO 3 , water, 2% citric acid and water. The organic phase was dried and evaporated to dryness to yield a capped or N-protected peptide. 
     EXAMPLE 10 
     Method D: Carbobenzyloxy (CBZ--) Group Removal Procedure: 
     A solution of the CBZ-protected peptide or amino acid derivative (1 g) in ethyl acetate (15 mL) was mixed with 0.2 g of Pd/C on carbon (10% Pd on carbon containing 50% by weight of water) and hydrogenated for 4 h at 40 psi. The solution was filtered through Celite® (diatomaceous earth) and evaporated to dryness to yield the unprotected peptide or amino acid derivative. 
     EXAMPLE 11 
     Method E: Conversion of Acetal to Aldehyde 
     A solution of the peptide acetal (1 eq.) in 3 mL of THF was mixed with 3 mL of aqueous HCl (2M) and stirred for 0.5-2 h. The solvent was removed by evaporation and the final residue was diluted with water and lyophilized to give the peptide aidehyde. 
     EXAMPLE 12 
     Method F: Preparation of Leucinal Diethylacetal: ##STR6## Step 1: NMM (10 mL) was added to a solution of CBZ-Leu-OH (25 g, 93 mmol) in 250 mL of THF. The solution was cooled to -15° C., treated with 13 mL of isobutyl chloroformate (IBCF), and allowed to react for 10 min. Subsequently, a suspension of N,O--dimethylhydroxylamine hydrochloride (9.36 g, 96 mmol) in 40 mL of DMF and 10 mL of NMM was added. After 4 h of stirring, the mixture was diluted with 400 mL of EtOAc and the solution was consecutively washed with water, 5% NaHCO 3  solution, water, 2% citric acid, and water. The organic layer was dried over MgSO 4  and evaporated to yield 19.0 g of a colorless oil. 
     Step 2: A solution of the oil (19 g) from step 1, in 200 mL of ether was cooled to -78° C. under a nitrogen atmosphere, and 120 mL of a solution of lithium aluminiumhydride (LiAlH 4 )(1.0M) in ether was added dropwise over a period of 45 min. The solution was stirred for 30 min, and 200 mL of 1M potassium hydrogen sulfate (KHSO 4 ) was added dropwise under a nitrogen atmosphere. The organic layer was separated, washed with KHSO 4  (1M) solution, dried (MgSO 4 ) and evaporated to a colorless liquid, CBZ-Leucinal (CBZ-Leu-H). 
     Step 3:104 mL of triethyl orthoformate was added over a period of 30 min to a solution of CBZ-Leu-H (12 g) and p-toluenesulfonic acid (0.9 g) in 100 mL of anhydrous ethanol (EtOH). The mixture was stirred for 30 min, and then was evaporated and diluted with 500 mL of ether. The ether layer was washed consecutively with saturated solutions of NaHCO 3  and sodium chloride. The yellowish brown semisolid was recrystallized from cold hexane to yield off-white needles of CBZ-Leucinal diethylacetal. 
       1  H NMR (300 MHz, CDCl 3 ) δ: 7.28(m, 5H), 5.03(s, 2H), 4.77(d, 1H), 4.27(d, 1H), 3.8(m, 1H), 3.63(m, 2H), 3.43(m, 2H), 1.6(dd, 2H), 1.30(m, 2H), 1.12(m, 6H), 0.83(d, 6H). 
     Step4: The product (14.8 g) from step 3 was hydrogenated using method D, with 2.5 g of Pd/C to yield 4.09 g of leucinal diethylacetal as an oil. 
       1  H NMR (300 MHz, CDCl 3 ) δ:4.16(d, 1H), 3.70(m, 3H), 3.75(m, 2H), 2.87(m, 1H), 1.78(m, 1H), 1.33(m, 2H), 1.23(m, 6H), 0.935(dd, 6H). 
     EXAMPLE 13 
     Method G: Synthesis of P3 Mimics (see Schechter, I. and Burger, A. (1967) Biochem. Biophys. Res. Commun. 27:157-162 ##STR7## 
     Step 1: Preparation of benzyl cyclopentaneacetate: 
     A mixture of cyclopentylacetic acid (10.02 g, 8.2 mmol), benzyl alcohol (8.45 g, 78.2 mmol) and p-toluenesulfonic acid monohydrate (1.48 g, 7.82 mmol) in benzene (60 mL) was refluxed using a Dean-Stark water separator for 2 h. After cooling, benzene was removed and the mixture was diluted with ether (50 mL) and washed successively with saturated NaHCO 3  solution, saturated brine solution, dried and evaporated to give the compound (15.40 g) as an oil; 
       1  H NMR (300 MHz, CDCl 3 ) δ: 7.35(m, 5H), 5.10(s, 2H), 2.40(d, j=8 Hz, 2H), 2.26(m, 1H), 1.81 (m, 2H), 1.6 (m, 4H), 1.18(m, 2H). 
     Step 2: Preparation of benzyl 1-cyclopentyl-9-iododecanoate: 
     To a cooled (-78° C.) solution of lithium diisopropylamide (20 mmol) in a mixture of THF (20 mL) and hexane (8 mL) (obtained in situ from diisopropylamine and n-BuLi) was added slowly to the compound obtained in Step 1(3.96 g, 18 mmol) in anhyd. THF(10 mL). The mixture was stirred for 30 minutes and 1,8-diiodooctane (7.19 g, 20 mmol) in hexamethylphosphoramide (3.50 g, 20 mmol ), was added. The mixture was stirred at -78° C. for 30 minutes, slowly brought to 0° C. over a period of 2 h and quenched by the cautious addition of 50 mL of 12% aqueous sodium chloride solution. The mixture was extracted with ether, washed with brine, dried and the solvent was evaporated. The crude product was purified to an oil (3.23 g) by a flash chromatography over silica using hexane to 1% EtOAc in hexane as an eluant; 
       1  H NMR (300 MHz, CDCl 3 ) δ: 7.35(m, 5H), 5.15(s,2H), 3.20(t,8Hz,2H), 2.20(m, 1H), 2.0(m, 1H), 1.2-1.8 (m, 22H). 
     Step 3: Preparation of benzyl 9-cyano-1cyclopentyldecanoate: 
     A mixture of the iodoester from step 2, (3.99 g, 8.7 mmol) and sodium cyanide (0.47 g, 9.6 mmol) in 15 mL of anhyd. DMSO was heated at 70°-75° C. for 30 min. After cooling, the reaction mixture was poured over ice (˜40 g), extracted into ether and washed successively with water and saturated brine. The organic layer was concentrated to yield an oil (2.89 g). 
       1  H NMR (300 MHz, CDCl 3 ). δ: 7.35 (m, 5H), 5.15 (s, 2H), 2.30(t, 8Hz, 2H), 2.20(m, 1H), 2.00(m, 1H), 1.3-1.8 (m, 22H). 
     Step 4: Preparation of benzyl 9-N-phthalimido-1-cyclopentyldecanoate: 
     A mixture of the compound from step 2 (1.21 g, 2.6 mmol) and potassium phthalimide (0.536 g, 3 mmol) in 8 mL of DMF was heated at 70°-75° for 30 min. After cooling, the reaction mixture was poured over ice (˜40 g) and extracted into 60 mL of ether. The combined organic layer was washed with water and saturated brine and concentrated to a colorless oil (1.24 g). 
       1  H NMR (300 MHz, CDCl 3 ) δ: 7.85(m, 2H), 7.70(m, 2H), 7.35(m, 5H), 5.10(s, 2H), 3.65(t, 8Hz, 2H), 2.20(m, 1H), 2.00(m, 1H), 1.22-1.18(m, 22H). 
     Step 5: Preparation of 10-cyano-2-cyclopentyldecanoic acid: 
     A mixture of the cyanoester from step 3 (2.89 g, 8 mmol) and 10%, Pd-C (0.6 g, DeGussa, H 2  O content 50%) in anhyd MeOH (35 mL) was hydrogenated for 2 h (42-26 psi). The reaction mixture was filtered through a Celite® pad and concentrated to a colorless oil (2.02 g). 
       1  H NMR (300 MHz, CDCl 3 ) •2.35(t, 8 Hz, 2H), 2.20 (m, 1H), 2.00(m, 1H), 1.3-1.9 (m, 22H). 
     Step 6: Preparation of 2-cyclopentyl-10-N-phthalimido- decanoic acid: 
     Following the procedure of step 5, product (0.41 g) of step 4 was converted to the title compound. (0.31 g). 
       1  H NMR (300 MHz, CDCl 3 ). δ: 7.85 (m, 2H), 7.70 (m, 2H), 3.70(t, 8Hz, 2H), 2.15(m, 1H), 1.95 (m, 1H), 1.10-1.85 (m, 22H). 
     Step 7: Preparation of 10-amino-2-cyclopentyldecanoic acid benzyl ester. ##STR8## 
     A mixture of the ester from step 4(0.874 g, 1.7 mmol) and hydrazine monohydrate (0.425 g, 0.41 mL, 8.5 mmol) in methanol (8 mL) was heated under reflux for 30 min, concentrated, and the residue was triturated with ether to give a white precipitate which was filtered off. The filtrate was concentrated to give an oil (0.540 g). The oil was dissolved in CH 2  Cl 2  (8 mL) cooled to -10° C. and to it was added triethylamine (0.324 mL) followed by trifluoromethane sulfonylchloride (0.25 mL). The temperature was slowly brought to room temperature over a period of 1 h. The reaction mixture was then washed with water, 2% HCl, water and saturated brine. The organic layer was concentrated to an oil and was purified by flash chromatography over silica gel using 10% EtOAc in hexane as an eluant to yield 10-amino-2-cyclopentyl-decanoic acid benzyl ester as an oil (0.25 g). 
       1  H NMR (300 MHz, CDCl 3 ). δ: 7.35(m, 5H), 5.15(s, broad, 1H), 5.10(s, 2H), 3.25(m, 1H), 2.20(m, 1H),2.00(m, 1H), 1.10-1.7 (m, 22H). 
     Step 8: Preparation of 2-cyclopentyl-10(trifluoromethanesulfonamido)-decanoic acid: 
     Following the same procedure as described in step 5, the compound (0.25 g) obtained from step 7 was converted to the title compound (0.20 g) as an oil. 
       1  H NMR (300 MHz, CDCl 3 ). δ: 6.60-5.60 (b, 1H), 3.30 (d, 8Hz, 2H), 2.15(m, 1H), 1.90 (m, 1H), 1.1-1.8 (m, 22H). ##STR9## 
     Step 9: Preparation of t-butyl 8-iodocaprylic acid: 
     The title compound was synthesized following the procedure as described for the compound from step 2 from t-butyl acetate (1.16 g) and 1,6-diiodohexane (4.06 g) as an oil (2.13 g). 
       1  H NMR (300 MHz, CDCl 3 ) δ: 3.20(t, 8Hz, 2H), 2.20 (t, 8Hz, 2H),1.75-1.85(m, H), 1.55-1.65(m, 2H), 1.45(s, 9H), 1.25-1.43(m, 6H). 
     Step 10: Preparation of 2-cyclopentyldecan-1,10-dioic acid-1-benzyl-10-t-butyl ester. 
     Using the procedure as described in step 2, the ester (6.92 g) from step 1 and the iodoester (11.37 g) from step 9 were reacted to give the title compound (7.44 g) as an oil. 
       1  H NMR (300 MHz, CDCl 3 ) δ: 7.35 (m, 5H), 5.10 (s, 2H), 2.20(m, 3H), 2.00 (m, 1H), 1.45 (s, 9H), 1.0-1.9 (m, 20H). 
     Step 11: Preparation of 2-cyclopentyldecan-1,10-dioic acid-1-benzyl-10-methyl ester. 
     A solution of the diester from step 10 (2.86 g, 7 mmol) in CH 2  Cl 2  (30 mL) and 10 mL of 90% TFA was stirred for 30 min. The product obtained after evaporation was dissolved in a mixture of CH 2  Cl 2  (10 mL) and MeOH (6 mL). The solution was stirred at -20° C. and to it was slowly added thionyl chloride (6 mL) over a period of 2h. The solvent was evaporated and the residue was dissolved in diethyl ether (20 mL). The organic layer was washed successively with saturated NaHCO 3  solution, water and brine, dried, evaporated and flash chromatographed on silica gel (eluant: 2% EtOAc-hexane) to furnish the product as an oil (2.05 g). 
       1  H NMR (300 MHz, CDCl 3 ) δ: 7.35 (m, 5H), 5.10 (s, 2H), 3.65 (s, 3H), 2.30(t, 8Hz, 2H), 2.20(m, 1H), 2.00 (m, 1H), 1.10-1.90(m, 10H). 
     Step 12: Preparation of 2-cyclopentyldecan-1,10-dioic acid-10-methyl ester. 
     Using the procedure as described in step 5, the compound (4.96 g) from step 11 was converted to the title compound (3.66 g) as an oil. 
       1  H NMR (300 MHz, CDCl 3 ) δ: 3.65(s, 3H), 2.30(t, 8Hz, 2H), 2.15(m, 1H), 2.00 (m, 1H), 1.10-1.19 (m, 20H). 
     Step 13: Preparation of 6-cyanohexane-l-sulfinic acid sodium salt: ##STR10## 
     In a three necked flask, fitted with a water condenser, a mechanical stirrer and a dropping funnel was placed a solution of 1-bromo-6-cyanohexane (8.04 g, 42.3 mmol) in 75 mL of 95% EtOH was prepared. The mixture was heated to reflux and to it was slowly added a solution of sodium sulfite (8.00 g, 63.5 mmol) in 50 mL of H 2  O over a period of 30 min. The mixture was heated for 2 h and then concentrated under reduced pressure. The residue was dissolved in 95% EtOH (200 ml), heated to boiling and filtered. The filtrate was concentrated and cooled in an ice bath. The precipitate was collectedby filtration and dried to give 3.00 g of a white solid. The mother liquor on further concentration, produced another batch of 2.2 g of the product. 
       1  H NMR (300 MHz, DMSO-d 6 ) δ: 2.50 (t, j=8Hz, 2H), 2.40 (t, 8Hz, 2H), 1.55 (m, 4H), 1.3 (m, 4H). 
     Step 14: Preparation of 6-cyanohexane-1-sulfonyl chloride: 
     A mixture of the product from step 13 (0.340 g, 1.6 mmol), thionyl chloride (0.95 g, 8 mmol) and a drop of DMF was heated at 75°-80° C. for 30 min. After cooling, the solvent was removed and the residue was treated with ice-water (5 mL). The organic layer was extracted into 30 mL of ether and the combined organic layer was washed with 3% NaHCO 3  solution and water, dried (MgSO 4 ) and filtered. The filtrate was treated with active carbon (Darco), filtered and concentrated to give a colorless oil (0.240 g) which was used without further purification. 
     Examples 14-38 describe the syntheses of the MCP inhibitors listed in Table 5. The purification conditions characterizations for the MCP inhibitors are listed in Table 6. 
     
                                           TABLE 5__________________________________________________________________________Multicatalytic Protease Inhibitors ##STR11##ExampleNo.   R         n  W      X    Y         Z          IC.sub.50__________________________________________________________________________                                               nM14    NC        8               ##STR12##                     NO.sub.2                          (CH.sub.3).sub.2 CH                                    O          7/615    NC        8  &#34;      PMC  (CH.sub.3).sub.2 CH                                    O          2016    NC        8  &#34;      NO.sub.2                          (CH.sub.3).sub.2 CH                                    NNHCONH.sub.2                                               10017    NC        8  &#34;      NO.sub.2                          (CH.sub.3).sub.2 CH                                    NOH        . . .18    NC        8  &#34;      NO.sub.2                          (CH.sub.3).sub.2 CH                                    NOCH.sub.3 70019    NC        8  &#34;      NO.sub.2                          (CH.sub.3).sub.2 CH                                    NOCH.sub.2 C.sub.6 H.sub.5                                               . . .20    MeOOC     7  &#34;      PMC  (CH.sub.3).sub.2 CH                                    O          1021    MeOOC     7  &#34;      MTR  (CH.sub.3).sub.2 CH                                    O          3022    MeOOC     7  &#34;      PMC  CH.sub.3 CH.sub.2 CH.sub.2                                    O          11023    MeOOC     7  &#34;      NO.sub.2                          (CH.sub.3).sub.2 CH                                    O          524    C.sub.6 H.sub.4 (CO).sub.2 N           8  &#34;      NO.sub.2                          (CH.sub.3).sub.2 CH                                    O          225    CF.sub.3SO.sub.2 NH           8  &#34;      NO.sub.2                          (CH.sub.3).sub.2 CH                                    O          8/526    MeOOC     6  H      NO.sub.2                          (CH.sub.3).sub.2 CH                                    O          4027    MeOOC     6  H      NO.sub.2                          C.sub.6 H.sub.5                                    O          13028    MeOOC     6  H      PMC  (CH.sub.3).sub.2 CH                                    O          2029    MeOOC     6  H      PMC  (CH.sub.3).sub.2 CH                                    O          &gt;30030    MeOOC     6  H      PMC  CH.sub.3CH.sub.2 CH.sub.2                                    O          &gt;10031    MeOOC     6  H      TOS  (CH.sub.3).sub.2 CH                                    O          7532    MeOOC     6  H      MTR  (CH.sub.3).sub.2 CH                                    NNHCONH.sub.2                                               22033    MeOOC     6  H      MTR  (CH.sub.3).sub.2 CH                                    NNHCSNH.sub.2                                               70034    MeOOC     6  H      MTR  (CH.sub.3).sub.2 CH                                    O          8035    MeOOC     6  H      MTS  (CH.sub.3).sub.2 CH                                    O          40__________________________________________________________________________ ##STR13## ##STR14## ##STR15##ExampleNo.   R       m  n  W  X                     Y     IC.sub.50__________________________________________________________________________                                              nM36    NC      5  1  H  PMCNHC(NH)NH(CH.sub.2).sub.3                                        SO.sub.2                                              &gt;&gt;10037    β-Naphthyl         0  0  -- O.sub.2 NNHC(NH)NH(CH.sub.2).sub.3                                         ##STR16##                                              25038  ##STR17##         5  1  H  O.sub.2 NNHC(NH)NH(CH.sub.2).sub.3                                         ##STR18##                                              . . .__________________________________________________________________________ 
    
     
                                           TABLE 6__________________________________________________________________________                         HPLC gradient                                    Analyt.                         (Ret. time of the                                    rt. timeExample No.  COMPOUNDS              peak collected)                                    in min.                                        Mol. Wt                                             (M + H).sup.+__________________________________________________________________________14     10-cyano-2-cyclopentyldecanoyl-N.sup.g -nitro-L-                         45-75% B (10.63)                                    22.5                                        563.75                                             564  arginyl-L-leucinal15     10-Cyano-2-cyclopentyl-decanoyl-N.sup.g -                         43-100% B (22.0)                                    31.7                                        785.2                                             786  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-  L-arginyl-L-leucinal16     10-cyano-2-cyclopentanyl-decanoyl-N.sup.g -nitro-L-                         30-35% B 60 min                                    22.2                                        621  621  arginyl-L-leucinal semicarbazone                         (A = 30.0, B = 32.0)                                    22.717     10-cyano-2-cyclopentyl-decanoyl-N.sup.g -nitro-L-                         45-65% B (12.3)                                    24.8                                        578.7                                             579  arginyl-L-leucinal oxime18     10-cyano-2-cyclopentanyl-nonanoyl-N.sup.g -nitro-L-                         55-75% B   27.7                                        592.7                                             593  arginyl-L-leucinal-O-methyloxime                         (A = 8.9, B = 10.12)19     10-Cyano-2-cyclopentyl-decanoyl-N.sup.g -nitro-L-                         65-75% B   31.3                                        668.7                                             669  arginyl-L-leucinal-O-benzyloxime                         (A = 8.3, B = 9.5)20     9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N.sup.g -                         60-100% B (14.1)                                    32.0                                        804.11                                             805  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-  arginyl-L-leucinal21     9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N.sup.g -                         40-60% B (29.9)                                    28.9                                        749  750  (4-methoxy-2,3,6-trimethyl-benzene-1-sulfonyl)-  L-arginyl-L-leucinal22     9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N.sup.g -                         60-70% B (18.8)                                    32.2                                        804  804  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-  arginyl-L-norleucinal23     9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N.sup.g -                         40-70% B (13.98)                                    23.6                                        582.34                                             583  nitro-L-arginyl-L-leucinal24     2-cyclopentyl-10-N-phthalimido-decanoyl-N.sup.g -                         50-80% B (12.66)                                    27.89                                        683.86                                             684  nitro-L-arginyl-L-leucinal25     10-(trifluoromethanesulfonyl)amino-2-                         50-70% B   27.20                                        686.02                                             686  cyclopentyl-decanoyl-N.sup.g -nitro-L-arginyl-L-                         (A = 12.1, B = 12.9)                                    27.46  leucinal26     Monomethylazelayl-N.sup.g -nitro-L-arginyl-L-                         30-100% B (8.14)                                    17.06                                        501  501  leucinal27     Monomethylazelayl-N.sup.g -nitro-L-arginyl-L-                         10-100% B (16.9)                                    17.3                                        534  535  phenylalaninal28     Monomethylazelayl-N.sup.g -(2,2,5,7,8-                         10-100% B (11.7)                                    26.5                                        722  723  pentamethylchroman-6-sulfonyl)-L-arginyl-L-  leucinal29     Monomethylazelayl-N.sup.g -(2,2,5,7,8-                         40-70% B (16.2)                                    26.3                                        722  723  pentamethylchroman-6-sulfonyl)-D-arginyl-L-  leucinal30     Monomethylazelayl-N.sup.g -(2,2,5,7,8-                         50-60% B (14.0)                                    26.2                                        722  723  pentamethylchroman-6-sulfonyl)-L-arginyl-L-  norleucinal31     Monomethylazelayl-N.sup.g -(p-toluenesulfonyl)-L-                         40-50% B (19.6)                                    21.3                                        610  610  arginyl-L-leucinal32     Monomethylazelayl-N.sup.g -(4-methoxy,2,3,6-                         --         13.2                                        724  725  trimethyl-1-sulfonyl)-L-arginyl-L-leucinal  semicarbazone33     Monomethylazelayl-N.sup.g -(4-methoxy,2,3,6-                         --         23.4                                        741  741  trimethyl-1-sulfonyl)-L-arginyl-L-leucinal  thiosemicarbazone34     Monomethylazelayl-N.sup.g -(4-methoxy,2,3,6-                         40-44% B (60 min)                                    23.3                                        668  669  trimethylbenzene-1-sulfonyl)-L-arginyl-L-                         (16.9)  leucinal35     Methoxyazelaoyl-N.sup.g -(2,4,6-trimethylbenzene-1-                         40-60% B (14.1)                                    32.0                                        638  638  sulfonyl)-L-arginyl-L-leucinal36     6-Cyano-hexane-1-sulfonyl-N.sup.g -(2,2,5,7,8-                         45-75% B (13.74)                                    24.94                                        710.9                                             711  pentamethylchroman-6-sulfonyl)-L-arginyl-L-  leucinal37     2-Naphthoyl-N.sup.g -nitro-L-arginyl-L-leucinal                         35-55% B (9.16)                                    18.10                                        470.51                                             47138     CBZ-7-aminoheptanoyl-N.sup.g -nitro-L-arginyl-L-                         30-60% B   19.1                                        577  578  leucinal               (A = 13.3, B = 13.9)__________________________________________________________________________ HPLC conditions: Solvent A: Water containing 0.1% TFA Solvent B: Acetonitrile containing 0.1% TFA Detection: 215 nm. HPLC gradients are in 40 min. unless noted otherwise. Column Type: Zorbax R.sub.x -C.sub.8 - Dimension 4.6 mm × 250 mm Pore Size 80 Angstroms Particle Size 5 micron 
    
     EXAMPLE 14 
     10-cyano-2-cyclopentyldecanoyl-N g-  nitro-L-arginyl-L-leucinal ##STR19## 
     Step 1: Fluorenylmethyloxycarbonyl-N g  -nitro-L-arginyl-L-leucinal diethylacetal. 
     Fmoc-Arg (NO 2  )--OH (4.41 g, 10 mmol) was coupled with Leu-acetal (1.89 g, 10 mmol) according to Method A (Example 7), using 1.29 mL of IBCF, 2.2 mL of NMM and 10 mL of THF (in the place of DMF as in Method A of Example 7). The crude peptide was obtained as amorphous solid. 
       1  H NMR (300 MHz, CDCl 3  ). δ: 7.75 (d, 2H), 7.55(d, 2H), 7.4(m, 2H), 7.29(m, 2H), 6.21(d, 1H), 5.91(d, 1H), 4.30(m, 5H), 3.66(m, 3H), 3.63(d, 2H), 3.32(m, 2H),1.72(m, 4H), 1.29(m, 2H), 1.17(b, 6H), 0.89 (m, 6H). 
     Step 2: N g  -nitro-L-arginyl-L-leucinal diethylacetal. 
     The Fmoc group was removed from 3.5 g of the product from step 1 using deprotection Method B (Example 8). The free base (2.5 g) was obtained as semisolid. 
       1  H NMR (300 MHz, DMSO-d6). δ: 8.6(b, 1H), 7.61(d, 1H), 4.27(d, 1H), 3.90(m, 1H), 3.59(m, 1H), 3.45(m, 4H), 3.14(m, 2H), 1.54 (m, 4H), 1.33(m, 3H), 1.10(tt, 6H), 0.83(dd, 6H). 
     Step 3: 10-cyano-2-cyclopentyl-decanoyl-N g-  nitro-L-arginyl-L-leucinal diethylacetal. 
     According to Method C (Example 9), 10-cyano-2-cyclopentyl-decanoic acid (2 g, 7.5 mmol) from method G, in 16 mL of DMF was treated with the product of Step 2 (2.15 g, 5.5 mmol) using BOP (3.34 g, 7.5 mmol) and HOBt (1.01 g, 7.5 mmol) to yield the crude peptide (3.85 g) as a pale yellow solid. 
       1  H NMR (300 MHz, DMSO-d 6 ). δ: 8.31 (bd, 1H), 8.0 (bd, 1H), 7.83 (d, 1H), 4.34(m, 1H), 4.24(d, 1H), 3.91(m, 1H), 3.56(m, 4H), 3.43 (m, 2H), 3.16 (m, 2H), 2.47 (t, 3H), 2.03 (m, 1H), 1.76 (m, 2H), 1.15-2.0 (m, 25H), 1.07 (m, 6H), 0.81 (d, 6H). 
     Step 4 
     A solution of the product of the step 3 (0.2 g) in 10 mL of acetonitrile (ACN) containing 30% TFA was stirred for 1 h, and the solvent was evaporated and Compound 14 was precipitated using diethyl ether. The HPLC purification conditions are given in Table 6. 
       1  H NMR(300 MHz, DMSO-d 6 ) δ: 9.38(s, 1H), 8.56(b, 1H), 8.3(d, 1H), 7.99(dd, 1H), 4.38(m, 1H),4.17(m, 1H), 3.37(m, 4H), 3.17(m, 2H), 2.26(t, 3H), 1.0-2.0(m, 27H), 0.86(dd, 6H). 
     EXAMPLE 15 
     10-Cyano-2-cyclopenty1-decanoy1-N g-  (2,2,5,7,8-pentamethylchroman-6 -sulfonyl)-L-arginyl-L-leucinal ##STR20## 
     Step 1: 10-Cyano-2-cyclopentyl-nonanoyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), 10-cyano-2 -cyclopentyl-nonanoic acid (2 mmol, from step 5 in Method G of Example 13) was coupled with N g  -(2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal diethylacetal (1.8 mmol, from step 2 in Compound 14) to obtain the title compound as solid. 
     Step 2: 10-Cyano-2 -cyclopentyl-nonanoyl-N g-  (2, 2, 5, 7, 8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal. 
     Following Method E (Example 11), the product from step 1(0.3 g) was converted to Compound 15 (0.2 g) and purified by HPLC. 
       1  H NMR (300 MHz, DMSO-d 6 ) δ: 9.38(s, 1H), 8.23(d, 1H), 7.8(s, 1H), 7.72(d, 1H), 7.49(t, 1H), 7.48(s, 1H), 4.34(m, 1H), 4.14(m, 1H), 3.05 (m, 2H), 2.60 (t, 2H), 2.50 (s, 3H), 2.46 (s, 3H), 2.25(m, 4H), 2.00(s, 3H), 1.74(t, 2H), 1.6-1.0 (m, 25H), 1.13(s, 6H), 0.82 (dd, 6H). 
     EXAMPLE 16 
     10-cyano-2-cyclopentany1-decanoyl-N g-  nitro-L-arginyl-L-leucinal semicarbazone ##STR21## 
     Semicarbazide hydrochloride (0.20 mmol), sodium acetate (0.3 mmol) and water (0.5 mL) were added to a solution of Compound 14 (0. 050 g, 0.089 mmol) in 4.5 mL of ethanol and stirred overnight at room temperature. Solvent was removed and the resulting Compound 16 was purifiedby HPLC as indicated in Table 6. 
     1H NMR (300 MHz, DMSO-d 6 ). δ: 9.91 (s, 1H),8.51 (s, 2H), 8.04 (d, 1H), 7.95 (d, 1H), 7.40 (s, 1H), 7.09 (d, 1H), 6.29 (s, 2H), 4.44 (m, 1H), 4.33 (m, 1H), 3.16 (s, 2H), 2.08-1.00 (m, 33H), 0.87 (dd, 6H). 
     EXAMPLE 17 
     10 -cyano-2-cyclopentyl -decanoyl-N g-  nitro-L-arginyl-L-leucinal oxime ##STR22## 
     Hydroxylamine hydrochloride (0.037 g, 0.534 mmol) was added to a solution of the product of step 4 in Example 14 (0.05 g, 0.089 mmol) in pyridine (0.175 g, 2.2 mmol) at room temperature and then at 80° C. for 30 minutes. Compound 17 was isolated by HPLC as indicated in Table 6. 
     EXAMPLE 18 
     10-cyano-2-cyclopentany1-nonanoyl-N g-  nitro-L-arginyl-L-leucinal-O-methyloxime ##STR23## 
     Following the procedure of Example 17, Compound 18 was prepared using O-methylhydroxylamine hydrochloride (0.031 g, 0.38 mmol) and isolated as a mixture of two peaks by HPLC as indicated in Table 6. 
     EXAMPLE 19 
     10-Cyano-2-cyclopentyl-decanoyl-N g-  nitro-L-arginyl-L-leucinal-O-benzyloxime ##STR24## 
     Following the procedure as in the preparation of Example 17, Compound 19 was made using Compound 14 (0.05 g, 0.089 mmol), O-benzyl hydroxylamine hydrochloride (0.043 g, 0.267 mmol) and pyridine (0.092 mL, 1.14 mmol). The compound was separated as two peaks in HPLC purifications. 
     EXAMPLE 20 
     9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal ##STR25## 
     Step 1: Fmoc-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-argininyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), Fmoc-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginine (3.31 g, 5 mmol) was coupled with L-leucinal diethylacetal (0.85 g, from method F, Example 12) using BOP (2.21 g, 5 mmol), HOBt (0.67 g, 5 mmol) and NMM (0.7 mL) in 12 mL of DMF to yield the dipeptide acetal (3.24 g). 
       1  H NMR (300 MHz, CDCl 3 ). δ: 7.89 (s, 1H), 7.78 (d, 2H), 7.6 (d, 2H), 7.4 (t, 2H), 7.31 (t, 3H), 6.4 (d, 2H), 5.92 (d, 1H), 4.58 (m, 1H), 4.43 (m, 1H), 4.35 (m, 3H), 3.69 (m, 2H), 3.53 (m, H), 3.30 (m, 2H), 2.6 (t, 2H), 2.58 (s, 6H), 2.12 (s, 3H), 1.80 (tt, 2H), 1.64 (m, 3H), 1.32 (m, 10H), 1.18 (q, 6H), 0.89 (t, 6H). 
     Step 2: N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal. 
     Following Method B (Example 8), the Fmoc group was removed from the product (1.6 g) from step 1, to yield a semi-solid (1.3 g). 
       1  HNMR (300 MHz, DMSO-d 6 ) δ: 7.6(d, 1H), 6.76(b, 1H), 6.46(b, 1H), 4.27(d, 1H), 3.9 (m, 1H), 3.58 (m, 1H), 3.44 (m, 4H), 3.11 (t, 1H), 3.01(m, 2H), 2.58(t, 2H), 2.47(s, 6H), 2.03(s, 3H), 1.77(t, 2H), 1.5(m, 5H), 1.26(s, 6H),1.09(m, 6H), 0.83 (dd, 6H). 
     Step 3: 9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), 9-methyloxycarbonyl-2-cyclopentyl-nonanoic acid (0.56 g) was coupled with N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal diethylacetal (0.7 g, 1.1 mmol) using BOP (0.66 g, 1.5 mmol), HOBt (0.202 g, 1.5 mmol) and NMM (2.2 mL, 2 mmol) in 5 mL of DMF to get the product (1.8 g) as solid. 
       1  H NMR(300 MHz, DMSO-d 6 ) δ: 7.5-8.0(m, 2H), 6.66(m, 1H), 6.4(m, lH), 4.32(m, 1H), 4.24(d, 1H), 3.93(m, 1H), 3.57 (m, 6H), 3.44 (m, 4H), 3.04 (m, 2H), 2.59 (t, 2H), 2.48(s, 6H), 2.28(m, 3H), 2.03(s, 3H), 1.77(t, 2H), 1.48(m, 22H), 1.26(s, 6H), 1.1(tt, 9H), 0.81(dd, 6H). 
     Step 4: 9-Methoxycarbonyl-2-cyclopentyl-nonanyl-N g  -(2,2,5,7,8-pentamethylchroman-6-sulfonyl) -L-arginyl-L-leucinal. 
     According to the Method E (Example 11), the product (0.2 g) from step 1 was converted to Compound 20 and purified by HPLC directly. 
       1  H NMR(300 MHz, DMSO-d 6 ) δ: 9.37(s, 1H), 8.2-7.4(m, 2H), 6.69(drm, 1H), 6.4 (brm, 1H), 4.3 (m, 2H), 3.58 (s, 3H), 3.03 (m, 2H), 2.60(t, 2H), 2.48(s, 6H), 2.26(m, 4H), 2.03(s, 3H), 1.77(t, 2H), 1.47(brm, 28H), 1.24(s, 3H), 0.80 (dd, 6H). 
     EXAMPLE 21 
     9-Methoxycarbonyl-2-cyclopentyl-nonanyl-N g-  (4-methoxy-2,3,6-trimethyl-benzene-1-sulfonyl)-L-arginyl-L-leucinal ##STR26## 
     Step 1: 9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  (4-methoxy-2,3,6-trimethylbenzene-1-sulfonyl)-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), 9-Methoxycarbonyl-2-cyclopentyl-nonanoic acid (1.2 mmol, from step 12 in method G) was coupled with N g-  (4-methoxy-2,3,6-trimethylbenzene-l-sulfonyl)-L-arginyl-L-leucinal diethylacetal (1.0 mmol) to yield the peptide as solid. 
     Step 2: Following Method E (Example 11), the acetal from step 1 was converted to Compound 21 and purified by HPLC. 
       1  H (300 MHz, CDCl 3  /DMSO-d 6 ). δ: 9.42(s, 1H), 8.25(d, 1H), 7.92(d, 1H), 7.84(s, 1H), 6.4 (m, 2H), 4.40(m, 1H), 4.20(m, 1H), 3.80(s, 3H), 3.57 (s, 3H), 3.26(m, 2H), 2.64(s, 3H), 2.55(s, 3H), 2.36(m, 2H), 2.07(s, 3H), 1.8-1.0(m, 30H), 0.87 (dd, 6H) 
     EXAMPLE 22 
     9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-norleucinal ##STR27## 
     Step 1: 9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-norleucinal diethylacetal. 
     Using Method C (Example 9), 9-methoxycarbonyl--cyclopentyl-nonanoic acid (1 mmol) was coupled with N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-norleucinal diethylacetal (0.69 mmol, obtained from step 1 in Example 30 following method B) using BOP (1 mmol), HOBt (1 mmol) and NMM (2.5 mmol) in 5 mL of DMF. The reaction mixture was stirred for 4.5 hours and the peptide was isolated (0.37 g, 0.42 mmol). 
     Step 2: 9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-norl eucinal. 
     According to Method E (Example 11), the product (0.37 g, 0.42 mmol) from step 1 was converted to Compound 22 (0.33 g) and purified by HPLC. 
       1  H NMR(300 MHz, DMSO-d 6 ) δ: 9.29(s, 1H), 7.79(d, 1H), 7.89(d, 1H), 7.31(t, 1H), 7.26(s, 2H), 4.17(m, 1H),3.94 (m, 1H),3.49(s, 3H),2.94 (m, 2H), 2.74(t, 2H), 2.51(t, 2H), 2.37(s, 6H), 2.20(m, 4H), 1.94(s, 3H), 1.14(s, 6H), 1.0-1.8 (m, 3 H), 0.74 (t, 3H). 
     EXAMPLE 23 
     9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  nitro-L-arginyl-L-leucinal ##STR28## 
     Step 1: 9-Methoxycarbonyl-2-cyclopentyl-nonanoyl-N g-  nitro-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), 9-methoxycarbonyl-2-cyclopentyl-nonanoic acid (0.66 g, 0.33 mmol ) was coupled with the product from step 2 in Example 14 (0.109 g, 0.28 mmol) using BOP (0.146 g, 0.33 mmol), HOBt (0.0449 g, 0.33 mmol) and NMM (0.105, 0.95 mmol) in 7 mL of DMF to yield the title compound of Step 1 (0.124 g). 
       1  H NMR(300 MHz, CDCl  3  δ: 8.31(m, 2H), 6.69(br d, 1H), 6.15(br d, 1H), 4.5(m, 1H), 4.33(m, 1H), 4.18(m, 1H), 3.67(s, 3H), 3.53(m, 4H), 3.32(m, 2H), 2.68(m, 2H), 2.29(t, 3H), 2.0-1.0(b m, 34H) , 0.90 (dd, 6H). 
     Step 2: Following Method E (Example 11), the peptide (from step 1, 0.124 g) was converted to Compound 23 (0.106 g) . 
       1  H NMR (300 MHz, DMSO-d 6 ). δ: 9.39(s, 1H), 8.54(m, 1H), 8.31(d, 1H), 7.99(br d,2H), 4.36(m, 1H), 4.15(m, 1H), 3.33(s, 3H), 3.16(m, 2H), 2.27(t, 2H), 2.03(m, 2H), 1.0-1.7(m, 28H), 0.83(dd, 6H). 
     EXAMPLE 24 
     2cyclopentyl-10-N-phthalimido-decanoyl-N g-  nitro-L-arginyl-L-leucinal ##STR29## 
     Step 1: 2-cyclopentyl-10-N-phthalimido-decanoyl-N g-  nitro-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), the product (0.25 g, 0.65 mmol) from step 6 in Method G (Example 13) was coupled to the product from step 2 in Example 14 (0.195 g, 0.5 mmol) using BOP (0.288 g, 0.65 mmol), HOBt (0.088 g, 0.65 mmol) and NMM (0.130 mL, 0.130 mmol) 5mL of DMF to obtain the title compound (0.557 g) as solid. 
       1  H NMR (300 MHz, DMSO-d 6 ) δ: 8.57 (br m,, 1H), 7.91 (d, 1H), 7.85 (br d, 4H), 7.55 (d, 1H), 4.32 (m, 1H), 4.23 (dd, 1H), 3.90(m, 1H), 3.55 (t, 3H), 3.43 (m, 4H), 3.14(m, 2H), 2.0 (br m, 1H), 1.74 (br m, 2H), 2.0-1.15 (2 br m, 28H), 1.09 (tt, 6H), 0.79 (dd, 6H). 
     Step 2: 2-cyclopentyl-10-N-phthalimido-decanoyl-N g-  nitro-arginyl-L-leucinal. 
     Using Method E (Example 11), the product of step 1 (0.45 g) was converted to Compound 24 (0.32 g). 
       1  H NMR (300 MHz, DMSO-d 6 ). δ: 9.38 (s, 1H), 8.31 (d, 1H), 8.00 (d, 1H), 7.85 (m, 4H), 4.38 (m, 1H), 4.15 (1, 1H), 3.57 (tt, 3H), 3.15 (m, 2H), 2.00 (m, 1H), 1.9-1.0 (br m, 30H), 0.86 (dd, 6H). 
     EXAMPLE 25 
     10-(trifluoromethanesulfonyl)amino-2-cyclopentyl-decanoyl-N g-  nitro-L-arginyl-L-leucinal ##STR30## 
     Step 1: (Trifluoromethanesulfonyl)10-amino-2-cyclopentyl-decanoyl-N g  -nitro-L-arginyl-L-leucinal. diethylacetal 
     Using Method C (Example 9), 10-(trifluoromethanesulfonyl)-amino-2-cyclopentyldecanoic acid (0.199 g, 0.51 mmol) was coupled to the product from step 2 in Example 14 (0.175 g, 0.45 mmol) using BOP (0.22 g, 0.51 mmol), HOBt (0.069 g, 0.51 mmol) and NMM (0.052 mL, 0.47 mmol) in 5mL of DMF. The acetal was obtained as a solid (0.42 g). 
       1  HNMR (300 MHz, CDCl 3 ) δ: 6.06(br m, 1H), 5.81(m, 1H), 4.53(m, 1H), 4.32(d, 1H), 4.13(m, 1H), 3.73(q, 4H), 3.53(m, 2H), 3.30(q, 3H), 2.0-1.0(2 br m, 36H), 0.9(dd, 6H). 
     Step 2: 10-(trifluoromethanesulfonyl)amino-2-cyclopentyl-decanoyl-N g-  nitro-L-arginyl-L-leucinal. 
     Following Method E (Example 11), the product from step 1 (0.35 g) was converted to Compound 25 (0.17 g). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ: 9.27(s, 1H), 8.46(br m, 1H), 4.30(m, 1H), 3.87(m, 1H), 3.09(m, 5H), 2.8-1.0(2 br m, 30H), 0.78(dd, 6H). 
     EXAMPLE 26 
     Monomethylazelayl-N g-  nitro-L-arginyl-L-leucinal ##STR31## 
     Step 1: Monomethylazelayl-N g-  nitro-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), the title compound was made using azelaic acid monomethyl ester (0.708 g, 3.5 mmol), BOP (1.55 g, 3.5 mmol), HOBt (0.47 g, 3.5 mmol), NMM (0.38mL, 3.5 mmol), N g=  nitro-L-arginyl-L-leucinal diethylacetal (obtained from step 2 in Example 14 following method B, Example B) (1.306 g, 3.5mmol) in 12mL of DMF. The peptide was obtained as an amorphous solid (2.17 g) 1  H NMR (300 MHz, CDCl 3 ) δ: 6.58(d, 1H), 6.04(d, 1H), 4.47(m, 1H), 4.30(d, 1H), 4.17(m, 1H), 3.67(s, 3H), 3.53(m, 2H), 3.2-3.4(t, d, 2H), 2.3(m, 3H), 2.2 (t, 1H), 1.83(m, 1H), 1.2-1.8(m, 24H), 1.2(m,3H) ,0.9(d, 3H). 
     Step 2: Monomethylazelayl-N g-  nitro-L-arginyl-L-leucinal. 
     Using the Method E (Example 11), the peptide acetal (from step 1) (250mg) was converted to Compound 26 (0.22 g). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ: 9.38(s, 1H), 7.73(d, 1H), 4.30(m, 1H), 4.09(m, 1H), 3.57 (s, 3H), 3.15 (m, 2H), 3.01 (q, 1H), 2.75 (m, 1H), 2.24(m, 7H),1.50(m, 12H), 1.24(b, 14H), 0.86(m, 6H). 
     EXAMPLE 27 
     Monomethylazelayl -N g-  nitro-L-arginyl -L-phenylalaninal ##STR32## 
     Step 1. Phenylalaninal diethylacetal 
     CBZ-Phe-OH (6.0 g, 2 mmol) was converted to phenylalaninal-diethyl acetal following the same procedure used in the preparations of Leucinal diethylacetal (Method F, Example 12). 
     Step 2: Fmoc-Arg(NO 2 )--OH 
     A solution of fluorenylmethyloxycarbonyl-N-hydroxysuccinimidyl ester (32 mmol) in 60 mL of THF was added to a stirred solution of N g  -nitro-L-arginine (35 mmol) and NaHCO 3  (70 mmol) in 70 mL of H 2  O. The milky solution cleared after 1 h and the solution was acidified with solid citric acid to pH 2-3 and extracted with 300 mL of EtOAc. The organic layer was washed once with water, dried and evaporated to yield the title compound as white solid (26.8 mmol). 
     Step 3: Fmoc-Arg (NO 2 )-Resin. 
     A solution of 9-fluorenylmethyloxycarbonyl-N g  -nitro-L-arginine (from step 2, 26.8 mmol), BOP (30 mmol), HOBt (30 mmol) and NMM (55 mmol) in 40 mL of DMF was mixed with 10 g of PAC resin (α-methylphenacyl linker attached to polystyrene-1% divinylbenzene, substitution 0.97mmol/g, supplied by Bachem Bioscience, Philadelphia, Pa.) and stirred for 4 h. The resin was filtered off, washed with DMF, DCM and MeOH and dried to yield the final product Fmoc-Arg (NO 2  )-Resin (11.1 g). The resin, Fmoc-Arg (NO 2  )-Resin (11.1 g) was treated with 100 ml of a solution containing piperidine (30%), DMF (35%) and toluene (35%) and stirred for 2.5 hrs. The Fmoc removed resin was filtered and consecutively washed with DCM/DMF (50:50) and MeOH to yield the title product (9.2 g). 
     Step 4: MeOAz:Arg(NO 2 )-Resin. 
     Monomethyl azelate (20 mmol) was added to a stirred slurry of the Arg(NO 2 )-Resin (9.2 g), BOP (20 mmol), HOBt (20 mmol) and NMM (to adjust the pH to 8). After overnight stirring, a mixture of monomethylazelate(10 mmol), BOP (10 mmol), HOBt (10 mmol), and NMM (20 mmol) was added and stirred for 24 hrs. The resin was washed with DMF, DCM and methanol to yield 10.56 g of the resin. 
     Step 5: Monomethylazelayl-N g-  nitro-L-arginine. 
     The product from step 4(10.56 g) was stirred for 5 h in a solution of 100 mL of 67% DCM (30%) TFA and (3%) anisole. The slurry was filtered and the solvent was evaporated and triturated with ether to yield the peptide (1.11 g, 2.75 mmol). 
     Step 6: Monomethylazelayl-N g-  nitro-L-arginyl-L-phenylalaninal diethylacetal. 
     Following Method C (Example 9), methoxyazelayl-N g-  nitro-L-arginine (1 mmol) was coupled with L-phenylalaninal diethylacetal (1.3 mmol) using BOP (1.3 mmol), HBOt (1.3 mmol) and NMM (to adjust the pH to 8) to yield the title peptide (0.82 mmol). 
     Step 7: Monomethylazelayl-N g-  nitro-L-arginyl-L-phenylalaninal. 
     Using Method E (Example 11), product (0.82mmol) from step 6 was converted to Compound 27 (0.81 mmol). 
       1  H NMR (300 MHz, CDCl 3 ). •9.59(s, 1H), 8.36(s, 1H), 7.49 ( s, 1H), 7.31-7.09 (m, 7H), 6.71 (d, 1H), 4.69 (m, 1H), 4.60 (m, 1H), 3,66 (s, 3H), 3.41 (m, 2H), 3.27 (m, 2H), 2.31(t, 2H), 2.2(t, 2H), 1.80-1.2 (m, 14H) 
     EXAMPLE 28 
     Monomethylazelayl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal ##STR33## 
     Step 1: Monomethylazelayl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal. diethylacetal 
     Following Method C (Example 9), monomethylazelate (1.42 g, 7.0 mmol) was coupled with N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal diethylacetal (3.06 g, 5.0 mmol, obtained from step 2 in Example 20), using 7.0 mmol each of BOP, HOBt and NMM to obtain the compound (3.43 g) as solid. 
       1  H NMR (300 MHz, CDCl 3 ). δ: 6.58 (d, 1H), 6.04 (d, 1H), 5.4 (br, 1H), 5.06 (br, 1H), 4.47(m, 1H), 4.30(d, 1H), 4.17(m, 1H), 3.67(s, 3H), 3.53(m, 4H), 3.23(d, 1H), 3.19(t, 2H), 2.30(m, 2H), 2.2(tt, 2H), 2.0-1.25(2 br m, 17H), 1.20(tt, 6H), 0.90 (dd, 6H). 
     Step 2: Monomethylazelayl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal. 
     Using Method E (Example 11), the product (0.30 g) from step 1 was converted to Compound 28 (0.22 g). 
       1  H NMR (300 MHz, DMSO-d 6 ): δ: 9.38 (s, 1H), 8.5(br, 1H), 4.30(m, 1H), 4.09(m, 1H), 3.57(s, 3H), 3.15(m, 2H), 3.00(t, 2H), 2.75(m, 2H), 2.12(t, 2H), 1.8-1.20(2 br m, 17H), 0.86(dd, 6H). 
     EXAMPLE 29 
     Monomethylazelayl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-D-arginyl-L-leucinal ##STR34## 
     Step 1: 9-Fluorenylmethoxycarbonyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-D-arginyl-L-leucinal. diethylacetal 
     Following Method C (Example 9), 9-Fluorenylmethyloxycarbonyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-D-arginine (3 mmol) was coupled with leucinal diethylacetal (2.5 mmol) using BOP (3 mmol), HOBt (3 mmol) and NMM (5 mmol) in 5mL of DMF to yield the peptide (1.72 g, 2 mmol) as a solid. 
     Step 2: MeOAz-D-Arg (PMC)-Leucinal-diethylacetal. 
     Using Method C (Example 9), monomethyl azelate (1.0 mmol) was coupled with N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-D-arginyl-L-leucinal diethylacetal (0.54 g, 0.88 mmol) obtained from title product of Step 1 following Method B (Example 8), using BOP (1 mmol), HBOt (1 mmol) and NMM (3 mmol) in 5 mL of DMF to yield the product (0.735 g). 
     Step 3: Monomethylazelayl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-D-arginyl-L-leucinal. 
     Using Method E (Example 11), the product (0.1 g) from step 2 was converted to Compound 29 and purified by HPLC. 
       1  H NMR(300 MHz, CDCl 3 ) 9.49(s,1H), 7.74(t, 1H), 7.43(d, 1H), 6.43(d, 1H), 6.34(s, 2H), 4.60(m, 1H), 4.51(s, 3H), 4.54(s, 3H), 4.37(m, 1H), 3.66 (s, 3H), 3.31 (m, 2H), 2.63(t, 2H), 2.26(m, 4H), 2.09(s, 3H), 1.80(t, 2H), 1.57(m, 7H), 1.26(m, 16H), 0.90 (dd, 6H). 
     EXAMPLE 30 
     Monomethylazelayl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-norleucinal ##STR35## 
     Step 1: Fmoc-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-norleucinal. 
     Fmoc-Arg(PMC)-OH (2 mmol) was coupled with norleucinal diethylacetal (2 mmol, obtained from CBZ-Nle-OH following the procedures in the preparation of Leu-acetal) using BOP (2 mmol), HOBt (2 mmol) and NMM (6 mmol) in 6 mL of DMF, according to the Method C (Example 9), to yield the peptide (1.37 g, 1.64 mmol). 
     Step 2: MeOAz-Arg(PMC)-L-norleucinal diethylacetal. 
     Following Method C (Example 9), monomethylazelate (2 mmol) was coupled with Arg(PMC)-norleucinal diethylacetal (1.39 mmol, obtained from step 1 following Method B, Example 8) using BOP (2 mmol), HOBt (2 mmol) and NMM (6 mmol) and stirred overnight. The next day, 1 mmol each of monomethylazelate, BOP, HOBt and NMM were added and stirred for 4 hrs. The reaction mixture was worked-up as in Method C (Example 9), to get the peptide (0.37 g, 0.84 mmol). 
     Step 3: Following Method E (Example 11), the product from step 2 (0.94 g, 0.84 mmol) was converted to Compound 30 (0.58 g)  1  H NMR(300 MHz, CDCl 3 ) δ: 9.54 (s, 1H), 7.49(t, 1H), 6.74(d, 1H), 6.26(s, 2H), 6.23(d, 1H), 4.58 (m, 1H), 4.31(m, 1H), 3.66(s, 3H), 3.34(m, 2H), 2.63(t, 2H), 2.58(s, 3H), 2.56(s, 3H), 2.29(t, 2H), 2.23(t, 2H), 1.9-1.5 (m, 22H), 1.30(s, 6H), 0.86 (t, 3H). 
     EXAMPLE 31 
     Monomethylazelayl-N g-  (p-toluenesulfonyl)-L-arginyl-L-leucinal ##STR36## 
     Step 1: Fmoc-N g-  (p-toluenesulfonyl)-L-arginyl-L-leucinal diethylacetal. 
     Using Method C (Example 9), Fmoc-N g-  (p-toluenesulfonyl)-L-arginine (5 mmol) was coupled with leucinal diethylacetal (5.5 mmol) using HBTU (5.5mmol) , HOBt (5.5 mmol) and NMM (11 mmol) in 15 mL of DMF. The peptide was isolated as a solid (2.86 g, 3.96 mmol) 
     Step 2: Monomethylazelayl-N g-  (p-toluenesulfonyl)-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), monomethylazelate (6 mmol) was coupled with N g-  (p-toluenesulfonyl)-L-arginyl-L-leucinal diethylacetal (2.7 g, 4.7 mmol, obtained from Step 1 using method B, Example 8) using 1-benzotriazol-1-yl-1,1,3,3tetramethyluronium hexafluorophosphate (HBTU) (6 mmol), HOBt (6 mmol) and NMM (12 mmol) in 15 mL of DMF and stirring overnight. The reaction yield was boosted by adding monomethyl azelate (3 mmol) and diphenylphosphoryl azide (3 mmol) and stirred for 4 hours to yield the peptide as solid (1.92 g). 
     Step 3: Monomethylazelayl-N g-  (p-toluenesulfonyl)-L-arginyl-L-leucinal. 
     Following Method E (Example 11), the product from step 2 (1.92 g, 2.8 mmol) was converted to Compound 31 (1.64 g)  1  H NMR (300 MHz, DMSO-d 6 ). δ: 9.37(s, 1H), 8.97(d, 1H), 8.37(d, 1H), 7.66(d, 2H),7.37(m, 1H),7.29(d, 2H), 7.03(s, 1H), 6.63(s, 1H), 4.09 (m, 1H), 3.57 (s, 3H), 3.44 (m, 1H), 3.04 (m, 2H), 2.26(s, 3H), 2.33(t, 2H), 2.13(t, 2H), 2.80-1.09(m, 7H), 0.86 (dd, 6H). 
     EXAMPLE 32 
     Monomethylazelayl-N g-  (4-methoxy, 2,3,6-trimethyl-1-sulfonyl)-L-arginyl-L-leucinal semicarbazone ##STR37## 
     See Basak, A. et al., Int. J. Peptide Protein Res. 36 7-17 (1990). A mixture of MeOAz-Arg(MTR)-Leu-H (Example 34) (67mg, 0.10 mmol), semicarbazide hydrochloride (11 mg, 0.1 mmol ) and sodium acetate (9 mg, 0.11 mmol) in 3 mL of 90% EtOH was heated to 70° C. for 18 h. The reaction mixture was concentrated to afford a light yellow solid (Compound 32) which was subsequently purified by HPLC as in Table 6. 
       1  H NMR (300 MHz, CDCl 3 ) δ: 7.91(t, 1H), 7.09(d, 1H), 6.68(s, 1H),6.22(s, 1H), 4.37-4.46(m, 1H), 4.2-4.3(m, 1H), 4.04(d, 1H), 3.7(s, 3H), 3.58(s, 3H), 2.6(s, H), 2.27(dd, 2H), 2.05, 2.12(s, 3H), 1.2-1.64(m, 12H), 0.85(t, 6H). 
     EXAMPLE 33 
     Monomethylazelayl-N g-  (4-methoxy, 2,3,6-trimethyl-1-sulfonyl)-L-arginyl-L-leucinal thiosemicarbazone) ##STR38## 
     Following the same steps in Example 32, Compound 33 was made from the Compound of Example 34 (51 mg, 0.07 mmol) and thiosemicarbazide (7 mg, 0.07 mmol) in 2 mL of 90% EtOH. 
     EXAMPLE 34 
     Monomethylazelayl-N g-  (4-methoxy, 2,3,6-trimethylbenzene-1- sulfonyl)-L-arginyl-L-leucinal ##STR39## 
     Step 1: Following Method C (Example 9), 9-fluorenylmethyloxycarbonyl-N g-  (4-methoxy-2,3,6-trimethylbenzene-1-sulfonyl)-L-arginyl-L-leucinal 5 diethylacetal was prepared from 9-fluorenylmethyloxycarbonyl-N g-  (4-methoxy-2,3,6-trimethylbenzene-1-sulfonyl)-L-arginine and L-leucinal diethyl acetal. 
     Step 2: Monomethylazelayl-N g-  (4-methoxy-2,3,6-trimethylbenzenesulfonyl)-L-arginyl-L-leucinal. 
     Following Method C (Example 9), monomethyl azelate (6 mmol) was coupled with N g-  (4-methoxy2,5,6-trimethylbenzene-l-sulfonyl)-L-arginyl-L-leucinal diethylacetal (5 mmol, obtained from step 1 using Method B, Example 8) and the peptide was isolated as an amorphous solid (3.3 g). 
     Step 3: Methoxyazelayl-N g-  (4-methoxy-2,3,6-trimethylbenzene-1-sulfonyl)-L-arginyl-L-leucinal. 
     The product from step 2 (0.5 g) was converted to the Compound 34 (0.36 g) according to Method E (Example 11). 
       1  H NMR (300 MHz, CDCl 3 ). δ: 9.54 (s, 1H), 7.51(s, 1H), 6.74(d, 1H), 6.57(s, H), 6.40(s, 2H), 6.31 (s, 1H), 4.66 (m, 1H), 4.41 (m, 1H), 3.87 (s, 3H), 3.70(s, 3H), 3.33(m, 2H), 2.73(s, 3H), 2.66(s, 3H), 2.33(t, 2H), 2.26(t, 2H), 2.17(s, 3H), 2.00-1.26(m, 17H), 0.96(dd, 6H). 
     EXAMPLE 35 
     Methoxyazelayl-N g-  (2,4,6-trimethylbenzene-1-sulfonyl)-L-arginyl-L-leucinal ##STR40## 
     Step 1: Fmoc-N g-  (2,4,6-trimethylbenzene-1-sulfonyl)-L-arginyl-L-leucinal diethylacetal. 
     A solution of 4M HCl in dioxane (10 mL) was added to a solution of Boc-Arg(MTS)--OH (6 mmol) in 10 mL of dioxane. After 30 minutes, the solvent was removed and ether was added, the precipate was collected and dried (3.21 g, 9 mmol). The Arg(MTS)--OH hydrochloride was converted to Fmoc-Arg(MTS)--OH following the procedure as described for the preparation of the tosyl derivative (Example 31) to yield the title compound as a white solid (2.09 g). 
     Step 2: Fmoc-Arg(MTS)-Leu-acetal. 
     Following Method C (Example 9), Fmoc-Arg(MTS)--OH (3.361 mmol) was coupled with leucinal diethylacetal (4 mmol) using HBTU (4 mmol), HOBt (4 mmol) and NMM (10 mmol) in 15 mL of DMF to yield the title peptide (1.05 g). 
     Step 3: MeOAz-Arg(MTS)-Leu-acetal. 
     Using method C, monomethyl azelate (1.2 mmol) was coupled with Arg(MTS)-Leu-acetal (0.85 mmol, obtained from step 2 following Method B, Example 8) using BOP (1.2 mmol), HOBt (1.2 mmol) and NMM (3.6 mmol) in 3 mL of DMF and stirred overnight to give the title peptide as a semi-solid (0.59 g). 
     Step 4: Methoxyazelayl-N g-  (2,4,6-trimethylbenzene-1-sulfonyl)-L-arginyl-L-leucinal. 
     Using Method E (Example 11), the product (0.59 g) of step 3 was converted to Compound 35 (0.54 g) and purified by HPLC. 
       1  H NMR (300 MHz, CDCl 3 ). δ: 9.49(s, 1H), 7.53(s, 1H), 6.90(s, 2H), 6.81(d, 1H), 6.40(bs, 3H), 4.61(m, 1H), 4.38(m, 1H), 3.67(s, 3H), 2.67(s, 6H), 2.29(t, 2H), 2.20(t, 2H), 2.0-1.20(m, 19H), 0.91(dd, 6H). 
     EXAMPLE 36 
     6-Cyano-hexane-1-sulfonyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal ##STR41## 
     Step 1: 6-Cyano-hexane-1-sulfonyl-N g-  (2,2,5,7,8-pentamethylchroman-6- sulfonyl)-L-arginyl-L-leucinal. diethylacetal. 
     6-cyano-hexane-1-sulfonyl chloride (0.19 g,0.89 mmol) from step 13 in Method G (Example 13) was added to a solution of the product (0.55 g, 0.899 mmol) from step 2 in Example 20, in 1 mL of DMF and the pH of the solution was adjusted to 8 using NMM. After 4 hours of stirring, the reaction mixture was worked up as described in Method A (Example 7), to obtain the title compound (0.466 g). 
     Step 2: 6-Cyano-hexane-1-sulfonyl-N g-  (2,2,5,7,8-pentamethylchroman-6-sulfonyl)-L-arginyl-L-leucinal. 
     Using Method E (Example 11), the product (0.297 g) from step 1 was converted to Compound 36 (0.22 g) and purified by HPLC as described in the Table 6: 
       1  H NMR (300 MHz, DMSO-d 6  ): δ: 9.37 (s, 1H), 6.83(b, 1H), 6.43(b, 1H), 3.88(m, 1H), 3.77(m, 1H), 3.01(m, 2H), 2.80(m, 2H), 2.55(t, 2H), 2.46(s, 6H), 2.0(s, 3H), 1.75(m, 5H), 1.6-1.3(m, 15H), 1.23(s, 6H), 0.84 (dd, 6H). 
     EXAMPLE 37 
     2-Naphthoyl-N g-  nitro-L-erginyl-L-leucinal ##STR42## 
     Step 1: 2-Naphthoyl-N g-  nitro-L-arginyl-L-leucinal diethylacetal. 
     2-Naphthoylchloride (0.104 g, 0.55 mmol ) was added to a solution of the product from Step 2 in Example 14 in 2 mL of DMF and NMM (0.18 mL) and the title product was worked up to obtain the title compund as a solid (0.22 g). 
       1  H NMR (300 MHz, CDCl 3 ). δ8.92(b, 1H), 8.0-7.91(mm, 10H), 6.93 (d, 1H), 5.07(m, 1H), 4.37 (d, 1H ), 4.17 (m, 1H), 3.69 (m, 3H), 3.53 (m, 3H), 3.38(m, 2H) 1.77, 1.58, 1.4(mm, 5H), 0.83 (dd, 6H). 
     Step 2: 2-Naphthoyl-N g-  nitro-L-arginyl-L-leucinal. 
     Using Method E (Example 11), the product (0.1 g) from step 1 was converted to Compound 37 (60 mg).  1  H NMR (300 MHz, DMSO-d 6 ). δ: 9.43 (s, 1H), 8.72 (d, 1H), 8.53 (m, 2H), 8.0 (m, 6H),7.6 (m, 2H),6.20(m, 1H), 4.57 (m, 1H), 4.14 (m, 1H), 3.92 (m, 1H), 3.2 (m, 2H), 3.0 (d, 1H), 1.77 (m, 5H), 0.86 (m, 6H). 
     EXAMPLE 38 
     CBZ -7-aminoheptanoyl -N g  -nitro-L-arginyl-L-leucinal ##STR43## 
     Step 1: CBZ-7-aminoheptanoyl-N g-  nitro-L-arginyl-L-leucinal diethylacetal. 
     Following Method C (Example 9), CBZ-7aminoheptanoic acid (0.7 g, 2.5 mmol ) was coupled with the product of step 2 in Example 14 (0.78 g, 2.0 mmol) using BOP (1.1 g, 2.5 mmol), HOBt (0.34 g, 2.5 mmol) and NMM (0.253 mL, 2.5 mmol) to yield the peptide as a semi-solid, which was used directly in the next step. 
     Step 2: CBZ-7-aminoheptanoyl-N g-  nitro-L-arginyl-L-leucinal. 
     Following Method E (Example 11), the acetal from step 1 was converted to Compound 38 (0.8 g) after stirring the reaction for 5 h. 
       1  H (300 MHz, DMSO-d 6 ): δ: 9.30(s, 1H), 8.47(s, 1H), 8.46(s, 1H), 8.29(d, 1H), 8.11(t, 1H), 7.80 (s, 1H), 7.64(d, 1H), 7.31(s, 1H), 7.23(s, 5H), 4.91 (s, 2H), 4.23(m, 1H), 4.00 (m, 1H), 3.51(q, 2H), 3.27(m, 2H), 2.89(q, 2H), 2.11 (t, 2H), 2.03(t, 2H), 1.7-1.00(m, 10H), 0.77(dd, 6H) 
     Each of the published documents mentioned in this specification be herein incorporated by reference in their entirety. 
     Those skilled in the art will appreciate that numerous changes and modifications may be made to the preferred embodiments of the invention and that such changes and modifications may be made without departing from the spirit of the invention. It is therefore intended that the appended claims cover all equivalent variations as fall within the true spirit and scope of the invention. 
     
         __________________________________________________________________________SEQUENCE LISTING(1) GENERAL INFORMATION:(iii) NUMBER OF SEQUENCES: 10(2) INFORMATION FOR SEQ ID NO:1:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 36 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:TCGATCGAAGCTTGCCGCCACCATGGCGATGAAAGC36(2) INFORMATION FOR SEQ ID NO:2:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 30 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:AGCTAGCCTCGAGCAGATTACAGTTTAATG30(2) INFORMATION FOR SEQ ID NO:3:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 21 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:TTAATCCTCACTCTAAGAAAC21(2) INFORMATION FOR SEQ ID NO:4:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 24 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:TTGTACGGCCAGTGATGGAATGCT24(2) INFORMATION FOR SEQ ID NO:5:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 24 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:AGCATTCCATCACTGGCCGTACAA24(2) INFORMATION FOR SEQ ID NO:6:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 20 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:TAATACGACTCACTATAGGG20(2) INFORMATION FOR SEQ ID NO:7:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 41 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:GCACACCACTTTCATCGCCATGGTGGCGGCAAGCTTCGATC41(2) INFORMATION FOR SEQ ID NO:8:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 20 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:ATTAACCCTCACTAAAGGGA20(2) INFORMATION FOR SEQ ID NO:9:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 41 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:GATCGAAGCTTGCCGCCACCATGGCGATGAAAGTGGTGTGC41(2) INFORMATION FOR SEQ ID NO:10:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 18 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:CTTCAGTTAATCCTGTAA18__________________________________________________________________________