Abstract:
Cyclic hexapeptide somatostatin analogs are prepared wherein a peptide surrogate replaces eight of the ring amino acids of somatostatin. The cyclic hexapeptides are easier to synthesize, have a longer duration of activity, and many have a greater level of activity than somatostatin. The compounds have the properties of inhibiting the release of glucagon, growth hormone and insulin. Certain of the compounds also are capable of inhibiting the release of gastric acid secretions. The compounds are particularly useful in the treatment of acromegaly, diabetes, diabetic retinopathy and peptic ulcers. These cyclic hexapeptides are prepared by the solid phase method.

Description:
BACKGROUND OF THE INVENTION 
     Somatostatin is a tetradecapeptide incorporating a cyclic dodecapeptide, having the structure: ##STR1## and has the properties of inhibiting the release of growth hormone, inhibiting the release of insulin and glucagon and reducing gastric secretions. Somatostatin itself has a short duration of action because it is inactivated, inter alia, by aminopeptidases and carboxypeptidases present in vivo. This problem of the short duration of action has been partially solved in the prior art by preparing derivatives of somatostatin which have low solubility, thus attaining a slow release on subcutaneous injection. Once dissolved, however, the derivatives are no more stable to inactivation by aminopeptidases and carboxypeptidases than somatostatin itself. 
     SUMMARY OF THE INVENTION 
     The present invention provides for cyclic hexapeptides which are derivatives of somatostatin in which, inter alia, eight of the ring amino acids are replaced by an amino acid, containing a peptide bond surrogate and both of the exocyclic amino acids are removed. Further substitution and reaction of the remaining amino acids is also described. The cyclic hexapeptides inhibit the release of glucagon, growth hormones and insulin, and inhibit the release of gastric acid secretions. Specifically the compounds may preferentially inhibit the release of growth hormones without affecting the level of gastric secretions or without affecting the level of gastric secretions, insulin and glucagon, or the compounds may inhibit the release of gastric acid secretions. Thus, the compounds have a more selective biological activity than somatostatin. The cyclic hexapeptide structure of the instant compounds also have a longer duration of activity than somatostatin. As such the instant cyclic hexapeptides are useful for the treatment of acromegaly, diabetes, diabetic retinopathy and peptic ulcers. 
     Thus, it is an object of the present invention to describe the cyclic hexapeptide somatostatin analogs. A further object is to describe procedures for the preparation of such cyclic hexapeptides. A still further object is to describe the use of such compounds in the treatment of acromegaly, diabetic retinopathy and peptic ulcers. Further objects will become apparent from reading the following description. 
     DESCRIPTION OF THE INVENTION 
     The compounds of the instant invention are best realized in the following structural formula: ##STR2## wherein 
     R 1  and R 2  are independently loweralkyl, benzyl, substituted benzyl where the substitutent may be one or two of loweralkyl, halogen, hydroxy, amino, nitro or loweralkoxy; and loweralkyl substituted with a 5- or 6- membered heterocyclic ring; 
     R 3  is 3-indolymethyl or substituted 3-indolylmethyl wherein the substituent may be loweralkyl, loweralkoxy, or halogen; 
     R 4  is loweralkyl, hydroxyloweralkyl, benzyl, carboxyloweralkyl, aminoloweralkyl or substituted benzyl wherein the substituent may be loweralkyl, loweralkoxy, hydroxy, halogen, amino or nitro; 
     R 5  is a hydrogen, loweralkyl, benzyl, or substituted benzyl wherein the substituent is loweralkyl, lower alkoxy, hydroxy, halogen, amino or nitro; 
     R 6  and R 7  are independently hydrogen or methyl 
     R 8  is aminocyclohexylmethyl, aminomethylbenzyl, preferably 4-aminocyclohexylmethyl and 4-aminomethylbenzyl, or ##STR3## wherein Y is (CH 2 ) m  and m is 0, 1 or 2 or sulfur such that the sulfur may be in any position along the chain; and the peptide surrogate linkage W--X is --CH 2  --S--, --CH 2  --S(O)----,CH 2  --SO 2  --, --CH 2  --NH--, --CH 2  --O--, --S--CH 2  --, --CH 2  --CH(OH)--, --CH 2  --CH 2  -- and --CH 2  --CO--. 
     The term &#34;loweralkyl&#34; when used in the instant application is intended to represent those alkyl groups either straight or branched chain, which have from 1-5 carbon atoms. Examples of such alkyl groups are methyl, ethyl, propyl, iso-propyl, butyl, sec-butyl, pentyl and the like. 
     The term &#34;loweralkoxy&#34; is intended to include those alkoxy groups of from 1-5 carbon atoms, in either a straight or branched chain. Examples of such alkoxy groups are methoxy, ethoxy, propoxy, isopropoxy, butoxy, tert-butoxy, pentoxy and the like. 
     The term &#34;halogen&#34; or &#34;halo&#34; is intended to include fluorine, chlorine, bromine and iodine. 
     The term &#34;5- or 6-membered heterocyclic ring&#34; is intended to include those 5- and 6-membered heterocycles with 1- or 2-heteroatoms selected from oxygen, nitrogen and sulfur. Exemplary of such heterocycles is imidazole, furan, thiazole, pyrazole, pyridine and the like. 
     In the instant compounds there are several assymetric centers which will lead to the existence of optical isomers for such compounds. In the instant invention, for each of the assymetric centers of the various amino acids which make up the instant cyclic hexapeptides, both the D and L configurations are intended to be encompassed. 
     It will be appreciated by those skilled in the art that when R 1  and R 2  are benzyl, R 3  is indolylmethyl, R 4  is 1-hydroxyethyl, R 6  and R 7  are hydrogen, and R 8  is CH 2  --CH 2  --CH 2  CH 2  NH 2 , the 7, 8, 9, and 10 amino acids of somatostatin (-Phe-Trp-Lys-Thr-) are represented, and the peptide surrogate amino acid, represented by 5-amino-6-phenyl-3-thiahexanoic acid when W is methylene, X is S, has taken the place of the remainder of the somatostatin amino acids. Thus, using the above definitions of the substituent groups, the following representative cyclic hexapeptide analog of somatostatin is formed in structure I; ##STR4## 
     The preferred embodiments of the cyclic hexapeptides of this invention are realized in the foregoing structural formula I wherein the peptide surrogate --W--X-- is --CH 2  --S--, and --CH 2  --S(O)--; 
     R 1  and R 2  are as defined above; 
     R 3  is 3-indolymethyl or substituted indolylmethyl wherein the substituent is methoxy or fluoro; 
     R 4  is methyl, ethyl, hydroxy methyl or hydroxy ethyl; 
     R 5  is hydrogen or methyl; 
     R 6  and R 7  are hydrogen; and 
     R 8  is CH 2  --CH 2  --CH 2  --CH 2  NH 2 . 
     Further preferred embodiments are realized when the peptide surrogate --W--X-- is as defined above; 
     R 1  and R 2  are as defined above; 
     R 3  is 3-indolymethyl; 
     R 4  is hydroxyethyl; and 
     R 5  is hydrogen; 
     R 6  and R 7  are hydrogen; and 
     R 8  is --CH 2  --CH 2  --CH 2  --CH 2  NH 2 . 
     The preferred R 1  and R 2  groups are loweralkyl, benzyl or substituted benzyl where the substituent is loweralkyl, halogen, hydroxy, amino, nitro or alkoxy. 
     Included within these preferred compounds are: 
     Cyclo-(Pheψ[CH 2  S]Gly-Tyr-D-Trp-Lys-Thr) 
     Cyclo-(Pheψ[CH 2  S]Gly-Phe-D-Trp-Lys-Thr) 
     Cyclo-(Pheψ[CH 2  S]Gly-Phe-L-Trp-Lys-Thr) 
     Cyclo-(Pheψ[CH 2  S]Gly-Tyr-D-Trp-Lys-Val) 
     Cyclo-(Pheψ[CH 2  S]Gly-Tyr-Trp-Lys-Val) 
     Cyclo-(Pheψ[CH 2  S]Gly-Phe-D-Trp-Lys-Ser) 
     Cyclo-(Pheψ[CH 2  SO]Gly-Tyr-D-Trp-Lys-Thr) 
     Cyclo-(Pheψ[CH 2  SO]Gly-Phe-D-Trp-Lys-Thr) 
     Cyclo-(Pheψ[CH 2  SO]Gly-Phe-L-Trp-Lys-Thr) 
     Cyclo-(Pheψ[CH 2  SO]Gly-Tyr-D-Trp-Lys-Val) 
     Cyclo-(Pheψ[CH 2  SO]Gly-Tyr-Trp-Lys-Val) 
     Cyclo-(Pheψ[CH 2  SO]Gly-Phe-D-Trp-Lys-Ser) 
     The terminology -AAψ[W-X]AA- is used to indicate replacement of the amide linkage between amino acids (AA) with the surrogate W-X. (See A. F. Spatola in Chemistry and Biochemistry of Amino Acids, Peptides and Proteins, B. Weinstein (Ed.), Marcel Dekker, Inc., New York, pg. 267-357 (1984). 
     In the instant application several abbreviated designations are used for the amino acid components, certain preferred protecting groups, reagents and solvents. The meanings of such abbreviated designations are given in Table I. 
     
                       TABLE I______________________________________AbbreviatedDesignation______________________________________           Amino AcidLys             L-lysinePhe             L-phenylalanineTrp             L-tryptophanD-Trp           D-tryptophanThr             L-threonineAha             7-aminoheptanoic acidTyr             L-tyrosineVal             L-valineAbu             L-α-aminobutyric acidSer             L-serineGly             GlycineAsn             L-asparaginePro             L-prolineAsu             D- or L-aminosuberic acidCys             L-cysteineAChxAla         aminocyclohexylalanineAmPhe           aminophenylalanine           Protecting GroupsINOC            isonicotinyloxycarbonylBOC             tert-butyloxycarbonylOMe             methyl esterBu              tert-butylCbz             benzyloxycarbonylBzl             benzyl2-Cl--Cbz       2-chlorobenzyloxycarbonylAcm             acetamidomethylMe              methylAc              acetate           Activating GroupsONp             p-nitrophenyl esterHSE             N--hydroxysuccinimide esterHBT             1-hydroxybenzotriazole           Condensing AgentsDCCI            dicyclohexylcarbodiimide           ReagentsTFA             trifluoroacetic acidTEA             triethylamineDIPEA           diisopropylethylamineDMAP            dimethylaminopyridine           SolventsEPAW            ethyl acetate-pyridine-           acetic acid-waterBAW             butanol-acetic acid-waterCMW             chloroform-methanol-waterDMF             dimethylformamideTHF             tetrahydrofuran______________________________________ 
    
     In accordance with the present invention, the novel cyclid hexapeptide somatostatin analogs are prepared by cyclizing corresponding linear peptides. The linear peptides are prepared by using the slid phase sequential synthesis technique. Accordingly, the process for preparing the cyclic hexapeptide somatostatin analogs of the present invention comprises (a) preparing a corresponding blocked linear peptide attached to a solid phase resin; (b) selectively deblocking the N-terminal amine group; (c) removing the linear peptide from the resin; (d) treating the linear peptide with a cyclizing agent to obtain the cyclic hexapeptide through the formation of an amide bond; (e) removing any side chain blocking groups. 
     When the linear peptide is prepared on the resin, it is generally not critical which amino acid is selected to be at the C-terminal position provided only that the sequence of amino acids in the linear peptide corresponds to that in the desired somatostatin analog. Once a linear peptide has been cyclized one can no longer determine which amino acid was at the C-terminus of the linear peptide. 
     While generally the selection of the first amino acid to start the chain is not critical, since the linear peptide will be cyclized, there may be other factors which may prefer one starting amino acid over another. For example D-Trp can react with t-butyl carbonium ions which are formed when BOC groups are removed. Thus, selection of a reaction sequence which places D-Trp at the N-terminal end of the linear peptide will cause D-Trp to be added last, and thus it will have the least exposure to t-butyl carbonium ions. This type of selection may not always be possible, such as where there are two indole containing moieties or other groups with reactivity to carbonium ions such as the thiomethylene peptide surrogate in the peptide. However, such reaction sensitivities should be considered when planning a peptide reaction sequence. 
     The synthesis of the linear peptides by the solid phase technique is conducted in a stepwise manner on chloromethylated resin. The resin is composed of fine beads (20-70 microns in diameter) of a synthetic resin prepared by copolymerization of styrene with 1 to 2 percent divinylbenzene. The benzene rings in the resin are chloromethylated in a Friedel-Crafts reaction with chloromethyl methyl ether and stannic chloride. The Friedel-Crafts reaction is continued until the resin contains 0.5 to 5 mmoles of chlorine per gram of resin. 
     The amino acid selected to be the C-terminal amino acid of the linear peptide is converted to its amino protected derivative. The carboxyl group of the selected C-terminal amino acid is bound covalently to the insoluble polymeric resin support, as for example, as the carboxylic ester of the resin-bonded benzyl chloride present in chloromethyl-substituted polystyrene-divinylbenzene resin. After the amino protecting group is removed, the amino protected derivative of the next amino acid in the sequence is added along with a coupling agent, such as dicyclohexylcarbodiimide. The amino acid reactant may be employed in the form of a carboxyl-activated amino acid such as the ONp ester, an amino acid azide, and the like. Deprotection and addition of successive amino acids is performed until the desired linear peptide is formed. In the case of the peptide surrogate, the adjacent amino acids and the peptide surrogate are added as a single unit of the structure AAψ[W-X]AA, which will be appropriately protected as described below. 
     The selection of protecting groups is, in part, dictated by particular coupling conditions, in part by the amino acid and peptide components involved in the reaction. 
     Amino-protecting groups ordinarly employed include those which are well known in the art, for example, urethane protecting substituents such as benzyloxycarbonyl (carbobenzoxy), p-methoxycarbobenzoxy, p-nitrocarbobenzoxy, t-butyloxycarbonyl, and the like. It is preferred to utilize t-butyloxy carbonyl (BOC) for protecting the α-amino group in the amino acids undergoing reaction at the carboxyl end of said amino acid. The BOC protecting group is readily removed following such coupling reaction and prior to the subsequent step by the relatively mild action of acids (i.e. trifluoroacetic acid, or hydrogen chloride in ethyl acetate). 
     The OH group of Thr and Ser can be protected by the Bzl group and the ε-amino group of Lys can be protected by the INOC group, the benzyloxycarbonyl (Cbz) group or the 2-chlorobenzyloxycarbonyl (2-Cl-Cbz) group. In the case of Lys, it is preferred to protect the ε-amino group with 2-Cl-Cbz or Cbz group as these groups are removed simultaneously with the Bzl groups by treatment with HF after the linear peptide has been cyclized. The INOC group is not removed by HF and requires an additional treatment with Zn. None of the groups are affected by TFA, used for removing BOC protecting groups. After the linear peptide is cyclized, the protective groups, such as 2-Cl-Cbz and Bzl, are removed by treatment with HF. 
     After the linear peptide has been formed on the solid phase resin, it may be removed from the resin by a variety of methods which are well known in the art. For example the peptide may be cleaved from the resin with hydrazine and thus directly form the peptide hydrazide which may be subsequently cyclized via the azide to the desired cyclic peptide. The hydrazide is converted to the corresponding azide by reaction with a reagent which furnishes nitrous acid in situ. Suitable reagents for this purpose include a lower alkyl nitrite (e.g. t-butyl nitrite, isoamyl nitrite) or an alkali metal nitrite salt (e.g., sodium nitrite, potassium nitrite) in the presence of a strong acid such as hydrochloric, phosphoric, etc. This reaction is carried out in the presence of either water and/or a non-aqueous solvent such as dimethylformamide, tetrahydrofuran, dioxane, chloroform, methylene chloride, etc., at a temperature between about -40° C. and +20° C. Alternatively, the peptide may be removed from the resin by treatment with a lower alcohol such as methanol in the presence of an organic base such as triethylamine, thus resulting in the formation of the corresponding lower alcohol ester of the linear peptide. The resulting ester may be converted to the hydrazide which may then be cyclized, via the azide, to the desired cyclic peptide. The preferred method for cleaving the peptide from the resin in the present invention is the use of hydrazine. 
     As reference Table II will show, one preferred overall procedure for preparing the desired cyclic peptides of the present invention involves the stepwise synthesis of the linear peptide on a solid phase resin. More specifically, in the process for preparing: ##STR5## the carboxyl end of the N-blocked amino acid lysine is bound covalently to an insoluble polymeric resin support as the carboxylic acid ester of the resin-bonded benzyl chloride. The α-amino group of Lys is protected by the BOC group and the ε-amino group is protected by the Cbz group. After the attachment of the Lys is completed on the resin, the protecting group BOC is removed by treatment with TFA in CH 2  Cl 2 . The subsequent amino acids are attached, in the form of BOC-amino acid, using DCCI as the condensing agent. After the desired linear peptide has been prepared, the N-terminal amino group is selectively deblocked and the peptide is removed from the resin by treatment with hydrazine. The resulting linear peptide hydrazide with the N-terminal amino group deblocked having the amino acid sequence: ##STR6## is treated with isoamyl nitrite in acid pH to form the corresponding azide. The azide solution is diluted with solvent and neutralized with an organic base. The linear peptide cyclizes to form: ##STR7## During the cyclization the &#34;pH&#34; is checked and maintained at neutral by the addition of organic base. The &#34;pH&#34; in organic solvent is determined by the application of an aliquot of the solution to moistened narrow range pH paper. 
     After the linear peptide is cyclized, the protective groups, Cbz and OBzl, are removed by treatment with HF in the presence of anisole. The crude cyclic peptide obtained is purified chromatographically, preferably with column chromatography on silica gel. The elution solvent is generally an organic solvent or mixtures thereof which is selected by analyzing aliquots of the material using thin layer chromatography. 
     
                       TABLE II______________________________________Reaction scheme for preparing: ##STR8##______________________________________ClCH.sub.2 φ-resin ##STR9##BOCLys(Cbz)OCH.sub.2 φ-resin ##STR10##BOCThr(Bzl)D-Pheψ[CH.sub.2 S]GlyPheD-TrpLys(Cbz)OCH.sub.2φ-resin ##STR11##HThr(Bzl)D-Pheψ[CH.sub.2 S]GlyPheD-TrpLys(Cbz)OCH.sub.2φ-resin ##STR12##HThr(Bzl)D-Pheψ[CH.sub.2 S]GlyPheD-TrpLys(Cbz)NHNH.sub.2 ##STR13##HThr(Bzl)D-Pheψ[CH.sub.2 S]GlyPheD-TrpLys(Cbz)N.sub.3 ##STR14##Cyclo(Thr(Bzl)D-Pheψ[CH.sub.2 S]GlyPheD-TrpLys(Cbz)) ##STR15##Cyclo-(D-TrpLysThrD- Pheψ[CH.sub.2 S]GlyPhe)______________________________________ 
    
     The following Examples are given to illustrate the methods used to carry out the present invention. It is to be understood that these Examples are given for purposes of illustration and not limitation. 
     EXAMPLE 1 
     Preparation of H-Thr(Bzl)-D-Pheψ[CH 2  S]Gly-Phe-D-Trp-Lys(Cbz)OCH 2  -φ-resin 
     Chloromethyl resin (2% cross-linked Merrifield resin), 862.0 g. (2.37 moles), having 2.75 meq. chlorine/g., and 900.6 g. (2.37 moles, 1 equivalent) of BOC-Lys(Cbz)OH were added to 4320 ml. of peroxide-free tetrahydrofuran. The mixture was stirred in an oil bath at 80° C. bath temperature for 45 minutes. Triethylamine, 310.0 ml., was added and the reaction mixture stirred at 80° C. bath temperature for 70 hours, cooled to 25° C. and transferred to a stirred solid phase reaction column with 2000 ml. of tetrahydrofuran. After removal of the solvent, the resin was washed using the stirred column with: 
     3×2000 ml. of tetrahydrofuran 
     4×5170 ml. of ethanol 
     1×5170 ml. of acetic acid 
     3×5170 ml. of water 
     3×5710 ml. of methanol 
     3×5170 ml. of chloroform 
     The BOC-Lys(Cbz)-O-CH 2  φ-resin was dried in vacuo at 25° C. for 16 hours, giving 1105 g. of BOC-Lys(Cbz)-O-CH 2  φ-resin containing 0.6 mmole of lysine/g. resin. 
     BOC-Lys(Cbz)-O-CH 2  φ-resin (3.33 g.; 2.0 mmole) was carried through the procedures in Tables III and IV using 2 deblockings (2 minutes and 25 minutes) with 40% TFA in methylene chloride plus 1% ethanediol and 2.5 equivalents of BOC-amino acid in the required sequence until the desired BOC-hexapeptide-O-CH 2  φ-resin was obtained. 
     DCCI was used as the sole coupling agent in every step. 
     The coupling of each amino acid proceeded smoothly. Best yields were obtained when the coupling was repeated in each step. When the coupling was repeated, the initial three methylene chloride washes, the deblocking step and the succeeding three methylene chloride washes were all omitted and replaced by a single methylene chloride wash. 
     The coupling reactions were carried out in methylene chloride, freshly degassed DMF or a mixture of these two solvents. The N-terminal amino group was blocked with a BOC group in each case; the hydroxy group of Thr was blocked with Bzl and the ε-amino group of Lys with Cbz. 
     When the desired BOC-hexapeptide-O-CH 2  φ- resin was obtained, the N-terminal BOC group was removed by the terminal deblocking procedure set forth in Table V. 
     
                                           TABLE III__________________________________________________________________________Solvent or  CH.sub.2 Cl.sub.2       40% TFA             CH.sub.2 Cl.sub.3 (3)                   NEt.sub.3 --                        CH.sub.2 Cl.sub.2 (6)                              BOC AA                                    0.5 M DCCI                                           MeOHreagent  (3)  in          CH.sub.2 Cl.sub.2                              in    in     CH.sub.2 Cl.sub.2(number of  CH.sub.2 Cl.sub.2 +                   (1:9)      CH.sub.2 Cl.sub.2                                    CH.sub.2 Cl.sub.2                                           (3 eachtreatments  1% ethane-             DMF or a     alternatively)or washes)  diol                   mixture of       (2)                    bothVol. in ml.  40   20    40    40   40    25    10     40Time in min.   5   2 and 25              2    10    2     2     5      2                                    coupling 30__________________________________________________________________________ 
    
     
                       TABLE IV______________________________________Protected Amino Acid   Solvent Ml.______________________________________BOC--D-Trp (1.52 g.)   20 ml. CH.sub.2 Cl.sub.2,Recouple                5 ml DMFBOC Phe (1.33 g.)      25 ml. CH.sub.2 Cl.sub.2RecoupleBOC--D-Pheψ[CH.sub.2 S]Gly (1.63 g.)                  25 ml. CH.sub.2 Cl.sub.2RecoupleBOC--Thr(Bzl) (1.55 g.)                  25 ml. CH.sub.2 Cl.sub.2Recouple______________________________________ 
    
     
                       TABLE V______________________________________DEBLOCKINGPROGRAM       TERMINAL______________________________________Solvent CH.sub.2 Cl.sub.2 (1)             40% TFA in  CH.sub.2 Cl.sub.2                                MeOH(2)or reagent        CH.sub.2 Cl.sub.2 + 1%                         (3)    CH.sub.2 Cl.sub.2 (1)(number of        Ethanedithiol      MeOH(2)treatments        (2)                CH.sub.2 Cl.sub.2 (2)or washes)Vol. in ml.   40        40          40     40Time in  5        2 and 25     2      2minutes______________________________________ 
    
     After the procedures of Tables III, IV and V were completed, the blocked hexapeptide-OCH 2  φ-resin is dried overnight and weighs 5.2 g. 
     EXAMPLE 2 
     Preparation of H-Thr(Bzl)D-Pheψ[CH 2  S]Gly-Phe-D-Trp-Lys(Cbz)-NHNH 2   
     The resin from Example 1 (1.8 g) was combined with 60 ml. of a 1:1 mixture of methanol and hydrazine and stirred at room temperature for 1 hour. The reaction mixture was evaporated to dryness in vacuo and the crystalline residue concentrated 3 times from methanol. The residue was suspended in 1:1 methanol-water, filtered and washed with the same mixture of solvents. The solid material was dried, affording 2.35 g. of the hydrazide which was used in the next step. 
     EXAMPLE 3 
     Preparation of H-Thr(Bzl)-D-Pheψ[CH 2  S]Gly-Phe-D-Trp-Lys(Cbz)-N 3   
     The product from Example 2 (2.3 g, 2.24 mmole) was combined with 30 ml. of degassed dimethylformamide under a blanket of nitrogen and cooled to -10° C., and 5 equivalents of 6.8M. hydrogen chloride (1.65 ml, 11.22 mmole) in tetrahydrofuran (1.7 ml.) was added. The solution was cooled to -25° C. and 9.5 ml. of a 1:19 mixture of isoamyl nitrite in dimethylformamide is added. The completion of the reaction is followed by thin layer chromatography and the disappearance of the hydrazide starting material. 
     EXAMPLE 4 
     Preparation of Cyclo(D-Trp-(ε2-Cl-Cbz)Lys-(O-Bzl)Thr-D-Pheψ[CH 2  S]Gly-Phe) 
     The solution of azide compound of Example 3 was added to 900 ml. of degassed dimethylformamide, precooled to -25° C., and the pH adjusted to 8. Over the next 2 hours, an additional 0.3 ml of triethyl amine was added to maintain the pH at 8. The mixture was stored for 16 hours at -20° C. and 16 hours at 3° C. Thin layer chromatography indicates that the reaction was complete. The mixture was concentrated to dryness and partitioned between methylene chloride and 0.5M citric acid (100 ml of each). The methylene chloride was washed with 0.5M citric acid (100 ml), water (100 ml) and 1N sodium bicarbonate (100 ml). Saturated sodium chloride was used in the first and third washes to supress emulsions. The solution was dried over sodium sulfate, and while drying, after 10 days, a solid formed which floated to the surface and proved to be the product. 
     EXAMPLE 5 
     Preparation of Cyclo(D-Trp-Lys-Thr-D-Pheψ[CH 2  S]-Gly-Phe) 
     0.741 Mg. (0.75 mmoles) of the protected cyclic hexapeptide of Example 4 was combined in a teflon lined chamber with 4 ml. of anisole. The chamber was then evacuated and filled with liquid hydrogen fluoride at the temperature of the dry ice/acetone bath. The temperature was raised to 0° C. and stirring continued for 1 hour. The hydrogen fluoride is allowed to evaporate and the residue placed in vacuo until a slurry is formed. The slurry is treated with ethyl acetate and filtered affording 0.5794 g. of a fine powder. 
     Following the above procedure, and by modifying only the selection and order of amino acids in the process of Example 1, there are prepared other cyclic hexapeptides of this invention, such as the following: 
     Cyclo(D-Trp-Lys-Thr-Pheψ[CH 2  S]Gly-Phe) 
     Cyclo(D-Trp-Lys-Thr-D-Pheψ[CH 2  SO]Gly-Phe) 
     Cyclo(Trp-Lys-Thr-D-Pheψ[CH 2  S]Gly-Phe) 
     Cyclo(D-Trp-Lys-Thr-Pheψ[CH 2  SO]Gly-Phe) 
     Cyclo(Trp-Lys-Thr-Pheψ[CH 2  S]Gly-Phe) 
     Cyclo(Trp-Lys-Thr-Pheψ[CH 2  SO]Gly-Phe) 
     Cyclo(D-Trp-Lys-Val-Pheψ[CH 2  S)Gly-Tyr) 
     Cyclo(Trp-Lys-Val-Pheψ[CH 2  S]Gly-Tyr) 
     Analogs of somatostatin were compared to somatostatin in their ability to decrease the levels of portal vein glucagon and insulin in anesthetized rats. Male Sprague-Dawley rats (Charles River CD) weighing 160-200 g were anestetized with urethane (150 mg/100 g of body weight; Aldrich). Saline or peptides were administered via the external jugular vein. After 5 minutes, the portal vein was exposed, and blood was collected via syringe containing 3 mg of EDTA and placed in chilled tubes containing 100 μ of Trasylol (FBA Pharmaceuticals) for subsequent hormone analysis. Plasma levels of glucagon were determined by the method of Faloona and Unger, Methods of Hormone Radioimmunossay, Jaffe and Behrman (Eds), Academic Press, New York, Vol. II, pp. 257-527 (1976), utilizing glucagon antisera 30 K obtained from R. Unger (Dallas, Tex). Plasma levels of insulin were determined by a modification of the procedure of Herbert et al., J. Clin. Endocrinol. Metab., 25, 1375-1384 (1965). 
     The test results for some of the compounds of this invention are recorded below with the results for somatostatin listed first and given the arbitrary value of 1. The results for the instant compounds are given as multiples or fractions of the effect of somatostatin. The numbers in parentheses are the fiducial limits for the number preceding. The first of the instant compounds listed is the compound prepared in Example 1-5. The compound is written slightly different, however, to conform to the order of the amino acids found in somatostatin. 
     
         ______________________________________Activity of Cyclichexapeptide Analogs of Somatostatin            Growth            Hormone    Insulin            Release    Inhi-   GlucagonCompound         Inhibition bition  Inhibition______________________________________Somatostatin     1          1       1Cyclo(D-Pheψ(CH.sub.2 S)--            0.06       0.2     0.2Gly--Phe--D-Trp--Lys--Thr)            (0.03, 0.10)Cyclo(D-Pheψ[CH.sub.2 S(O)]--            0.01       --      --Gly--Phe--D-Trp--Lys--Thr)(Sulfoxide Diastereomer A)Cyclo(D-Pheψ[CH.sub.2 S(O)]--             0.004     --      --Gly--Phe--D-Trp--Lys--Thr)            (0.001, 0.009)(Sulfoxide Diastereomer B)Cyclo(L-Pheψ[CH.sub.2 S]--            1.36       1.5     1.11Gly--Phe--D-Trp--Lys--Thr)            (0.58, 4.50)                       (0.7,   (0.2, 4.5)                       3.0)Cyclo(D-Pheψ[CH.sub.2 S]--            --          0.07   0.07Gly--Phe--L-Trp--Lys--Thr)______________________________________