Abstract:
A new and distinctive variety of the kiwi plant,  Actinidia deliciosa , named ‘Tsechelidis’ is described. The new variety is characterized by very large oblong fruit covered with downy hairs, and very broad ovate leaves having acuminate apexes, among other features. The size and uniformity of the fruit provide significantly higher yield than other known varieties of kiwi.

Description:
Latin name of the genus and species of the plant claimed: 
   
     Actinidia deliciosa.  
   
   Variety denomination: ‘Tsechelidis’. 
   BACKGROUND OF THE INVENTION 
   Kiwi plants have been cultivated outside their native China for over one hundred years. Known varieties or cultivars include Hayward, Meteor, Hort16A, Abbott, Tomua, Jade Moon, Bruno, Monty, Matua and Kuimi. Hayward is the most popular variety worldwide. Kiwi plants are now commercially grown in New Zealand, Italy, Chile, France, Greece, Japan, China and the United States. 
   Kiwi plants are commercially grown for their oblong or oval fruit, having brown skin covered in short hairs. The flesh, firm until fully ripe, is glistening, bright green or sometimes yellow, brownish or off-white, except for the white, succulent center from which radiate many fine, pale lines. Between these lines are scattered minute dark-purple or nearly black seeds, unnoticeable in eating. 
   Kiwi plants may be propagated by seed, grafting or cutting. 
   SUMMARY OF THE INVENTION 
   The present invention relates to a new and distinctive kiwi variety characterized by very large, oblong fruit covered with downy hairs, and very broad ovate leaves having acuminate apexes, among other features. The size and uniformity of the fruit provide significantly higher yield than other known varieties of kiwi. The new variety designated ‘Tsechelidis’ was derived from the ‘Hayward’ variety in Episkopi, Imathia, Greece and has been asexually reproduced by cutting, among other methods. 
   ‘Tsechelidis’ is further distinguished by the nutritional characteristics of the fruit as well as the qualitative characteristics. Furthermore, a molecular genetic analysis distinguishes ‘Tsechelidis’ from ‘Hayward’ as indicated by several polymorphisms in known alleles. 

   
     BRIEF DESCRIPTION OF THE PHOTOGRAPHS 
     The accompanying color photographs of ‘Tsechelidis’ show the new variety as well as comparisons of the ‘Tsechelidis’ variety to the ‘Hayward’ variety. 
       FIG. 1  shows a typical leaf of ‘Tsechelidis’. 
       FIG. 2  shows typical flowers of ‘Tsechelidis’. 
       FIG. 3  shows a typical flower of ‘Tsechelidis’ as compared to a typical flower of ‘Hayward’. 
       FIG. 4  shows typical fruit of ‘Tsechelidis’ on the vine. 
       FIG. 5  shows typical fruit of ‘Tsechelidis’ on the vine as compared to typical fruit of ‘Hayward’ on the vine. 
       FIG. 6  shows typical fruit of ‘Tsechelidis’ with stems attached. 
       FIG. 7  shows a cross-section and a stylar end view of a typical fruit of ‘Tsechelidis’ 
       FIG. 8  shows a cross-section and a side view of a typical fruit of ‘Tsechelidis’. 
       FIG. 9  shows typical fruit of ‘Tscehelidis’ as compared to typical fruit of ‘Hayward’. 
   

   DETAILED DESCRIPTION OF THE INVENTION 
   The present invention is a new and distinct variety of  Actinidia deliciosa  plants having the several characteristics that distinguish the variety from other kiwi plants, particularly the ‘Hayward’ variety. 
   The new variety ‘Tsechelidis’ was discovered in Episkopi, Imathia, Greece, when the inventor planted seeds from selected Hayward plants with the purpose of obtaining a group of male plants. From this original group of twenty plants, a single plant was identified as female. During its first harvest in 1994, the female plant bore unusually large fruit as compared to plants of the ‘Hayward’ variety. The female plant was monitored for the next two harvests and consistently bore the unusually large fruit, as well as other features which distinguished it from ‘Hayward’. The female plant was then used to propagate the ‘Tseehelidis’ variety by asexual reproduction beginning in 1996. 
   The ‘Tsechelidis’ variety has been continuously asexually reproduced by cuttings from 1996 through 2007. The distinguishing characteristics of the variety continue to run true in the propagated plants, as shown by about 1000 plants covering ½ hectare. Furthermore, no changes have appeared in the new variety when grafted on ‘Hayward’. In addition to cuttings, the new variety can be asexually reproduced by grafting to rootstocks of  A. deliciosa . 
   Male pollenizers suitable for ‘Tsechelidis’ may include, but are not limited to, ‘Mania’. At this time the ‘Tsechelidis’ variety is being tested for self-pollenization by the University of Volvos, Greece. According to preliminary results, ‘Tsechelidis’ is characterized by up to 75% self-pollenization as compared to 2% for ‘Hayward’. 
   ‘Tsechelidis’ should be cultivated in areas that are not affected by spring frost. Temperatures of less than −2° C. will damage the tender shoots of the plant and suspending growth or reduce the setting process. Also, frost in early autumn to late October may damage fruit situated on the vine. The presence of strong wind, particularly in May, may result in considerable decrease of flowers borne by the plant. 
   Distinguishing Characteristics from Known Varieties 
   The following is a comparison of the fruit production an orchard of ‘Tsechelidis’ as compared with cultivated ‘Hayward’ situated in the area of Episkopi-Anthemion in the prefecture of Imathia, Greece. At the time of this study (2006) the ‘Tsechelidis’ orchard was five years old. 
   The listed observations, measurements and assessments were made in the following categories:
         1. Plant and fruit characteristics   2. Quantitative production data (yield, number of fruits/plants and average fruit weight).   3. Level of nutritional elements in leaves and fruit.   4. Qualitative fruit characteristics (resistance to pressure, pH, soluble solids (° Brix), vitamin C, acids, sugars, Thiault index and dry matter).   5. Plant/Fruit disease and pest resistance/susceptibility
 
1. Plant and Fruit Characteristics:
       

   The chart below describes the physical differences between ‘Tsechelidis’ and the known ‘Hayward’ variety. The Horticultural terminology applied below is used in accordance with revised UPOV guidelines for kiwi (Test Guidelines—TG/98/6 — Actinidia  2001-04-04, available at http://www.upov.int/en/publications/tg-rom/tg098/tg_98_6. pdf). 
   
     
       
             
             
             
           
         
             
               TABLE 1 
             
             
                 
             
             
               UPOV Characteristics for 
                 
                 
             
             
               comparison of varieties 
               ‘HAYWARD’ 
               ‘TSECHELIDIS’ 
             
             
                 
             
           
           
             
               Plant: vigor 
               Medium 
               Strong 
             
             
               Leaf blade: shape 
               Broad ovate 
               Very broad ovate 
             
             
               Leaf blade: shape of apex 
               Acute 
               Acuminate 
             
             
               Leaf blade: green color of 
               Medium 
               Dark 
             
             
               upper side 
             
             
               Petal: curvature of apex 
               Strongly 
               Very strongly expressed 
             
             
                 
               expressed 
             
             
               Fruit: size 
               Large 
               Very large 
             
             
               Fruit: general shape 
               Ellipsoid 
               Oblong 
             
             
               Fruit: general shape of stylar 
               Flat 
               Between slightly  
             
             
               end 
                 
               depressed and flat 
             
             
               Fruit: shape of shoulder at 
               Rounded 
               Squared 
             
             
               stalk end 
             
             
               Fruit: type of hairiness 
               Hirsute 
               Downy 
             
             
               Time of beginning of 
               Late 
               Medium 
             
             
               flowering 
             
             
                 
             
           
        
       
     
   
   The shoots of ‘Tsechelidis’ grow more vigorously than ‘Hayward’, though there is no difference with respect to resilience against pest and diseases. The fruits of ‘Tsechelidis’ are more oblong than ‘Hayward’ (the ratio of fruit length/width is 1.41 and 1.24 respectively). Furthermore, the fruits of ‘Tsechelidis’ are larger and more uniform in size. ‘Tsechelidis’ do no require thinning absent defective fruit, unlike ‘Hayward’. Additionally, due to the larger fruit size, any decrease that may be caused by low temperatures during the flowering season or poor pollination of the fruits will not affect the marketability of the fruits, in contrast with ‘Hayward’. 
   In addition to the distinguishing features between ‘Tsechelidis’ and ‘Hayward’ listed above, the following characteristics were identified by the European Community Plant Variety Office (CPVO). Again the terminology is presented according to the revised UPOV guidelines for kiwi (Test Guidelines—TG/98/6— Actinidia  2001-04-04, available at http://www.upov.int/en/publications/tg-rom/tg098/tg_98_6.pdf). The UPOV characteristics are botanical terms known in the art for description plant varieties. 
   
     
       
             
             
             
           
             
             
             
           
         
             
               TABLE 2 
             
             
                 
             
             
               UPOV 
                 
               State of 
             
             
               No. 
               Characteristics 
               Expression 
             
             
                 
             
           
           
             
                 
             
           
        
         
             
               5 
               Young shoot: Hairiness 
               present 
             
             
               6 
               Young shoot: Density of hair 
               medium 
             
             
               7 
               Young shoot: Type of hairiness 
               hirsute 
             
             
               8 
               Young shoot: Anthocyanin coloration of 
               medium 
             
             
                 
               growing tip 
             
             
               9 
               Stem: Thickness 
               medium 
             
             
               11 
               Stem: Roughness of bark 
               medium-rough 
             
             
               12 
               Stem: Hairiness 
               present 
             
             
               13 
               Stem: Density of hair 
               medium 
             
             
               14 
               Stem: Type of hairiness 
               bristly 
             
             
               15 
               Stem: Size of lenticels 
               medium 
             
             
               16 
               Stem: Number of lenticels 
               medium 
             
             
               19 
               Stem: Size of bud support 
               small to medium 
             
             
               20 
               Stem: Profile of proximal face of bud 
               convex 
             
             
                 
               support (if sloping) 
             
             
               21 
               Stem: Presence of bud cover 
               present 
             
             
               22 
               Stem: Size of hole in bud cover 
               medium 
             
             
               23 
               Stem: Leaf sear 
               shallow 
             
             
               26 
               Leaf blade: Shape 
               very broad ovate 
             
             
               27 
               Leaf blade: Shape of apex 
               acuminate 
             
             
               28 
               Leaf blade: Arrangement of basal lobes 
               slightly apart 
             
             
               29 
               Leaf blade: Hair on upper side 
               medium 
             
             
               30 
               Leaf blade: Hair on lower side 
               medium 
             
             
               31 
               Leaf blade: Puckering/Blistering on 
               medium 
             
             
                 
               upper side 
             
             
               34 
               Leaf blade: Presence of variegation 
               absent 
             
             
               37 
               Leaf: Ratio petiole length/blade length 
               large 
             
             
               38 
               Petiole: Density of hair 
               medium-dense 
             
             
               39 
               Petiole: Anthocyanin coloration on 
               medium 
             
             
                 
               tipper side 
             
             
               40 
               Flower bud: anthocyanin coloration of 
               medium 
             
             
                 
               protruding petal end 
             
             
               41 
               Inflorescence: Predominant number of 
               one 
             
             
                 
               flowers 
             
             
               42 
               Flower stalk: Length 
               medium-long 
             
             
               43 
               Flower stalk: Density of hair 
               medium 
             
             
               44 
               Flower stalk: Length of hair 
               medium 
             
             
               45 
               Flower: Number of sepals 
               6 
             
             
               47 
               Sepal: Density of hair 
               sparse 
             
             
               48 
               Sepal: Length of hair 
               medium-short 
             
             
               49 
               Flower: Diameter 
               very large 
             
             
               50 
               Flower: Arrangement of petals (viewed 
               overlapping 
             
             
                 
               from beneath) 
             
             
               51 
               Petal: Curvature of apex 
               strongly expressed 
             
             
               52 
               Petal: Type of coloration (adaxial side) 
               single colored 
             
             
               54 
               Petal: Different shades of color 
               absent 
             
             
               60 
               Styles: Number 
               many 
             
             
               62 
               Styles: Attitude 
               erect and horizontal 
             
             
               63 
               Fruit: Size 
               very large 
             
             
               64 
               Fruit: General shape 
               oblong 
             
             
               65 
               Fruit: Shape in cross-section (at median) 
               elliptic 
             
             
               66 
               Fruit: General shape at stylar end 
               slight depressed 
             
             
                 
                 
               and flat 
             
             
               67 
               Fruit: Presence of calyx ring 
               weakly expressed 
             
             
               68 
               Fruit: Shape of shoulder at stalk end 
               squared 
             
             
               69 
               Fruit: Length of stalk 
               long 
             
             
               70 
               Fruit: Ratio stalk length/fruit length 
               large 
             
             
               71 
               Fruit: Persistence of sepals 
               present 
             
             
               72 
               Fruit: Conspicuousness of lenticels on skin 
               inconspicuous 
             
             
               74 
               Fruit: Hairiness of skin 
               present 
             
             
               75 
               Fruit: Density of hair 
               medium 
             
             
               76 
               Fruit: Type of hairiness 
               downy 
             
             
               77 
               Fruit: Distribution of hairs 
               evenly spread 
             
             
               79 
               Fruit: Adherence of hair to skin 
               medium-strong 
             
             
                 
               (when rubbed) 
             
             
               84 
               Fruit: Diameter of core relative to fruit 
               medium to large 
             
             
               85 
               Fruit: General shape of core (in cross 
               transverse elliptic 
             
             
                 
               section) 
             
             
               86 
               Fruit: Fluting of core (in cross section) 
               present 
             
             
               88 
               Fruit: Sweetness 
               medium-low 
             
             
               89 
               Fruit: Acidity 
               medium 
             
             
               90 
               Time of vegetative bud burst 
               medium 
             
             
               91 
               Time of beginning flowering 
               medium-late 
             
             
               92 
               Time of maturity for harvest 
               medium-late 
             
             
                 
             
           
        
       
     
   
   In addition to the above listed characteristics set forth according to the UPOV guidelines, the following measurements are typical of ‘Tsehedilids.’ The typical size of the leaves includes a stalk length of 12–14 cm, a length from stalk to apex of 18–20 cm and a leaf width of 16-18 cm. The typical flower diameter is 5–7 cm. The flowers are typically characterized by 5-6 sepals, 6-8 petals and 35-45 styles. The stalk length of the flowers typically ranges from 6–9 cm. 
   With regards to the fruit, the fruit length typically averages about 7.96 cm. The fruit width typically ranges from about 5.15 cm (small width) to about 5.71 cm (large width). The fruit weight typically ranges from 150-170 g. 
   The following color description has been provided according to the R.H.S. Colour Chart. 
                       TABLE 3               Characteristic Botanical Features   Color according to RHS Colour Chart                   Stem: Color of shoot on sunny side   178A-Greyed-Red Group       Stem: Color of lenticels   177C-Greyed-Orange Group       Leaf blade: Color of upper side   137B-Green Group       Leaf blade: Color of lower side   146C-YelIow-Green Group       Sepal: General color   200D-Brown Group       Petal: Main color on adaxial side   155D-White Group       Filament: Color   155C-White Group       Anther: Color   13C-Yellow Group       Styles: Color   158B-YelIow-White Group       Fruit: Color of skin   199A-Grey-Brown Group       Fruit: Color of hairs   199D-Grey-Brown Group       Fruit: Color of skin at maturity for   199A-GreyBrown Group       consumption       Fruit: Color of outer pericarp   141C-Green Group       Fruit: Color of inner pericarp   141C-Green Group       Fruit: Color of core   157D-Green-White Group                    
2. Quantitative Production Data:
 
   
     
       
             
             
             
             
           
             
             
             
             
           
         
             
               TABLE 4 
             
             
                 
             
             
                 
                 
               ‘HAY- 
               Significance 
             
             
               Parameter 
               ‘TSECHELIDIS’ 
               WARD’ 
               level (P) 
             
             
                 
             
           
           
             
                 
             
           
        
         
             
               Total number of fruits/plant 
               250 
               279 
               P &gt; 0.05 
             
             
               Number of marketable 
               249 
               222 
               P &gt; 0.05 
             
             
               fruits 
             
             
               Rate of marketable 
               99.6 
               79.6 
               — 
             
             
               fruits (%) 
             
             
               Total yield (kg/plant) 
               41.9 
               29.9 
               P &gt; 0.05 
             
             
               Yield of marketable fruits 
               41.6 
               25.1 
               0.001 &lt; 
             
             
               (kg/plant) 
                 
                 
               P &lt; 0.01 
             
             
               Yield of marketable fruits 
               99.3 
               83.9 
               — 
             
             
               (%) 
             
             
               Average weight of market- 
               167.0 
               114.5 
               P &lt; 0.001 
             
             
               able fruits (g) 
             
             
               Average weight of non- 
               237.0 
               88.8 
               P &gt; 0.001 
             
             
               marketable fruits (g) 
             
             
                 
             
             
               P &gt; 0.05 signifies a statistically insignificant difference. 
             
           
        
       
     
   
   ‘Tsechelidis’ has a greater density of buds in each stem than ‘Hayward’, whereby each stem, which has 13 buds, produces about 10 kg of fruit. Therefore, each ‘Tsechelidis’ tree, having about 15-18 stems, yields about 140-150 kg of fruit. The high yield, and large size and uniformity of the fruit of ‘Tsechelidis’, as compared to the ‘Hayward’, are significant advantages, particularly with regard to reducing production costs. This data was taken during a harvest affected by adverse weather during the growing season in Imathia, Greece. 
   3. Level of Nutritional Elements The following is table that shows the statistically significant differences in nutritional elements between ‘Tsechelidis’ and ‘Hayward’. 
                                                             TABLE 5                       Parameter   ‘TSECHELIDIS’   ‘HAYWARD’                                        Leaves: nitrogen level   1.95%       2.53%               Fruit skin: phosphorus   0.13%       0.08%           Fruit skin: potassium   2.35%   1.95%           Fruit skin: magnesium   0.08%       0.06%           Fruit skin: manganese   12.6   ppm   8.0   ppm           Fruit flesh: nitrogen   0.76%   0.95%           Fruit flesh: phosphorus   0.13%       0.16%           Fruit flesh: manganese   10.3   ppm   6.2   ppm           Fruit flesh: copper   6.79   ppm   10.51   ppm           Fruit flesh: proportion of   2.30       2.71           N/Ca                        
4. Qualitative fruit characteristics The following tables show specific qualitative fruit characteristics between ‘Tsechelidis’ and ‘Hayward’. The first table lists measurements of fruit immediately after harvest. The second table lists measurements taken of fruit held in refrigerated storage for two months after harvest.
 
   
     
       
             
             
             
             
           
             
           
             
             
             
             
           
             
           
             
             
             
             
           
         
             
               TABLE 6 
             
             
                 
             
             
                 
                 
                 
               Significance 
             
             
               Parameter 
               ‘TSECHELIDIS’ 
               ‘HAYWARD’ 
               level (P) 
             
             
                 
             
           
           
             
                 
             
           
        
         
             
               Measurements Taken During Harvest 
             
           
        
         
             
               Resistance to 
               23.0 
               27.0 
               0.001 &lt; 
             
             
               pressure (lb/in 2 ) 
                 
                 
               P &lt; 0.01 
             
             
               Flesh pH 
               3.34 
               3.25 
               0.0l &lt; P &lt; 0.05 
             
             
               Soluble solids 
               7.30 
               6.70 
               P &gt; 0.05 
             
             
               (°Brix)(%) 
             
             
               Vitamin C (mg/100 
               79.2 
               37.8 
               P &lt; 0.00l 
             
             
               g fresh weight) 
             
           
        
         
             
               Measurements Taken During Harvest 
             
           
        
         
             
               Malic acid (g/l) 
               4.5 
               4.0 
               P &gt; 0.05 
             
             
               Sugars (g/l) 
               62.8 
               57.2 
               0.01 &lt; 
             
             
                 
                 
                 
               P &lt; 0.05 
             
             
               Thiault index 
               107.7 
               97.6 
               0.01 &lt; 
             
             
                 
                 
                 
               P &lt; 0.05 
             
             
               Dry matter (%) 
               15.30 
               15.82 
               P &gt; 0.05 
             
             
                 
             
           
        
       
     
   
   
     
       
             
           
             
             
             
             
           
             
             
             
             
           
         
             
               TABLE 7 
             
           
           
             
                 
             
             
               Measurements Taken Two (2) Months After Harvest 
             
           
        
         
             
                 
                 
                 
               Significance 
             
             
               Parameter 
               ‘TSECHELIDIS’ 
               ‘HAYWARD’ 
               level (P) 
             
             
                 
             
           
        
         
             
               Resistance to 
               10. 
               10.9 
               P &gt; 0.05 
             
             
               pressure (lb/in 2 ) 
             
             
               Flesh pH 
               3.32 
               3.41 
               P &lt; 0.001 
             
             
               Soluble solids 
               13.6 
               13.0 
               P &gt; 0.05 
             
             
               (°Brix) (%) 
             
             
               Vitamin C (mg/100 
               80.2 
               38.3 
               P &lt; 0.001 
             
             
               g fresh weight) 
             
             
               Malic acid (g/l) 
               4.8 
               4.5 
               P &gt; 0.05 
             
             
               Sugars (g/l) 
               84.4 
               80.0 
               P &gt; 0.05 
             
             
               Thiault index 
               132.4 
               125.0 
               P &gt; 0.05 
             
             
                 
             
           
        
       
     
   
   Based on the above information, the following distinctions may be drawn between ‘Tsechelidis’ and ‘Hayward’. The fruit of ‘Tsechelidis’ ripen 7-10 days earlier than ‘Hayward’, which is shown by the fruit&#39;s lower resistance to pressure and their slight superiority in soluble solids (° Brix) during harvest. Despite the earlier ripening, the resistance to pressure of ‘Tsechelidis’ after two months of refrigerated storage was the same as ‘Hayward’. 
   5. Plant/fruit disease and pest resistance/susceptibility 
   There is no difference between ‘Tsechelidis’ and ‘Hayward’ with respect to resilience against pest and diseases. There are no other observed characteristics specific to plant/fruit disease and pest resistance/susceptibility. 
   Molecular Genetic Analysis 
   A molecular genetic analysis was conducted by Dr. Athanasios Mavromatis, Professor of Genetics &amp; Plant Breeding (University of Thessaly, School of Agricultural Services) comparing ‘Tsechelidis’ with ‘Hayward’ based on known microsatellite DNA markers using PCR. The method is recognized as an accurate and repeatable molecular analysis for  Actinidia . Huang, W.G., Cipriani, G., Morgante, M., Testolin, R. (1998) Microsatellite DNA in  Actinidia chinensis : isolation, characterization, and homology in related species. Theor. Appl. Genet. 97 (8): 1269-1278. 
   The DNA analysis was performed as follows: Repeatable samples of four genotypes were used (commercial clones of ‘Hayward’ (one female, one male) and ‘Tsechelidis’ (one female, one male)). Leaf samples of 0.3 g per genotype was used for DNA extraction process according to a modified cetyltrimethylammonium bromide (CTAB) method. The extract DNA was quantified on agarose gel by comparison with report samples (DNA marker). The DNA quality and quantity was tested to ensure accuracy of the molecular genetic analysis. 
   Thirteen known primer pairs were used for amplifying dinucleotide tandems AG/CT and AC/GT. The primer pairs used were: UDK 96-022, UDK 97-402, UDK 99-152, UDK 96-053, UDK 97-411, UDK 96-030, UDK 96-001, UDK 96-037, UDK 96-034. UDK 99-168, UDK 96- 092, UDK 97-406 and UDK 97-407. The primers were developed in a bilateral European Union International Cooperation with Developing Countries (INCO-DC) project performed by 
   University of Udine, Italy; Chinese Agricultural University, Beijing China; INRA, France; and University of Thessaly, Greece. 
   The sequence of all of the list primers are known and published the prior art. For example the sequences of UDK 96-001, UDK 96-022, UDK 96-030, UDK 96-034, UDK 96-037, UDK 97-402, UDK 97-406, UDK 97-407 and UDK-41 1 are all published in Huang et al., Microsatellite DNA in  Actinidia chinesis : isolation, characterization, and homology in related species. Theor. Appl. Genet. (1998) 97: 1269-1278. UDK 96-037 a and b listed below indicate the two polymorphic loci amplified in the same gel for the same primer UDK 96-037. The sequences of UDK 96-053 and UDK 99-152 are published in Korkovelos et al. Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight  Actinidia  species. Scientica Horticulturae 116 (2008) 305-310. UDK 96-092 and UDK 99-168 are also know primers as disclosed by Korkovelos et al. Screening microsatellites for their effectiveness to identify and differentiate among  Actinidia Genotypes . Acta Hort. 610 (2003) 357-363. 
   The PCR products were separated in 6% polyacrylamide gels 1.5 mm thick. Band visualization was made with silver nitrate. The results of the study indicated that at least seven out of 13 primer pairs were polymorphic. Therefore, the study provides grounds for distinguishing between genetic material from ‘Tsechelidis’ as compared to ‘Hayward’. 
   The polymorphic primer pairs are described in the table below according to the presence and absence of alleles of the same molecular weight. 
   
     
       
             
             
             
           
         
             
               TABLE 8 
             
             
                 
             
             
               DNA primer/alleles 
               ‘TSECHELIDIS’ 
               ‘HAYWARD’ 
             
             
                 
             
           
           
             
               99-152 
                 
                 
             
             
               97-411 
               + 
               − 
             
             
               96-030 
             
             
                   96-037a 
               − 
               + 
             
             
                96-037b 
               + 
               − 
             
             
               96-034 
               + 
               − 
             
             
               96-092 
               + 
               − 
             
             
               97-406 
               − 
               + 
             
             
                 
             
           
        
       
     
   
   The genetic difference confirmed through diverse binding patterns indicate that the ‘Tsechelidis’ variety is genetically distinct from ‘Hayward’.