Abstract:
According to the present invention, a series of genes are identified in Group B Streptococcus, the products of which may be associated with the outer surface of the organism. The genes, or functional fragments thereof, may be useful in the preparation of therapeutics, e.g. vacaccines to immunize a patient against microbial infection.

Description:
This application is a National Stage Application of International Application Number PCT/GB99/04377, filed Dec. 22, 1999. 
    
    
     FIELD OF THE INVENTION 
     This invention relates to the identification of bacterial genes and proteins, and their use. More particularly, it relates to their use in therapy, for immunisation and in screening for drugs. 
     BACKGROUND TO THE INVENTION 
     Group B Streptococcus (GBS), also known as  Streptococcus agalactiae , is the causative agent of various conditions. In particular, GBS causes: 
     Early Onset Neonatal Infection. 
     This infection usually begins in utero and causes severe septicaemia and pneumonia in infants, which is lethal if untreated and even with treatment is associated with a 10-20% mortality rate. 
     Late Onset Neonatal Infection. 
     This infection occurs in the period shortly after birth until about 3 months of age. It causes a septicaemia, which is complicated by meningitis in 90% of cases. Other focal infections also occur including osteomyelitis, septic arthritis, abscesses and endopthalmitis. 
     Adult Infections. 
     These appear to be increasingly common and occur most frequently in women who have just delivered a baby, the elderly and the immunocompromised. They are characterised by septicaemia and focal infections including osteomyelitis, septic arthritis, abscesses and endopthalmitis. 
     Urinary Tract Infections. 
     GBS is a cause of urinary tract infections and in pregnancy accounts for about 10% of all infections. 
     Veterinary Infections. 
     GBS causes chronic mastitis in cows. This, in turn, leads to reduced milk production and is therefore of considerable economic importance. 
     GBS infections can be treated with antibiotics. However, immunisation is preferable. It is therefore desirable to develop an immunogen that could be used in a therapeutically-effective vaccine. 
     SUMMARY OF THE INVENTION 
     The present invention is based on the identification of a series of genes in GBS, and also related organisms, the products of which may be localised on the outer surface of the organism and therefore may be used as a target for immuno-therapy. 
     According to one aspect of the invention, a peptide is encoded by an operon including any of the genes identified herein as pho1-13, pho3-21, pho2-15, pho3-18, pho3-22, pho3-3, pho3-17, pho2-2, pho1-5, pho3-1, pho3-23, pho3-50, pho1-14, pho2-10, pho3-14, pho3-24 and pho3-29, obtainable from Group B Streptococcus, or a homologue or functional fragment thereof. Such a peptide is suitable for therapeutic use e.g. when isolated. 
     The term “functional fragments” is used herein to define a part of the gene or peptide which retains the activity of the whole gene or peptide. For example, a functional fragment of the peptide may be used as an antigenic determinant, useful in a vaccine or in the production of antibodies. 
     A gene fragment may be used to encode the active peptide. Alternatively, the gene fragment may have utility in gene therapy, targetting the wild-type gene in vivo to exert a therapeutic effect. 
     A peptide according to the present invention may comprise any of the amino acid sequences identified herein as SEQ ID NOS. 2, 4, 6, 8, 10, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 and 35, or a functional fragment thereof. 
     Because of the extracellular or cell surface location, the peptides of the present invention may be suitable candidates for the production of therapeutically-effective vaccines against GBS. The term “therapeutically-effective” is intended to include the prophylactic effect of vaccines. For example, a vaccine may comprise a peptide according to the invention, or the means for its expression, for th treatment of infection. The vaccine may be administered to females prior to or during pregnancy to protect mother and neonate against infection by GBS. 
     According to another aspect of the invention, the peptides or genes may be used for screening potential antimicrobial drugs or for the detection of virulence. 
     A further aspect of this invention is the use of any of the products identified herein, for the treatment or prevention of a condition associated with infection by a Group B Streptococcal strain. 
     Although the protein has been described for use in the treatment of patients, veterinary uses of the products of the invention are also considered to be within the scope of the present invention. In particular, the peptides or the vaccines may be used in the treatment of chronic mastitis, especially in cows. 
    
    
     DESCRIPTION OF THE INVENTION 
     The present invention is described with reference to Group B Streptococcal strain M732. However, all the GBS strains and many other bacterial strains are likely to include related peptides or proteins having amino acid sequence homology with the peptide of M732. Organisms likely to contain the peptides include, but are not limited to,  S. pneumoniae, S. pyogenes, S. suis, S. milleri , Group C and Group G Streptococci and Enterococci. Vaccines to each of these may be developed in the same way as described for CBS. 
     Preferably, the peptides that may be useful for the production of vaccines have greater than 40% sequence similarity with the peptides identified herein. More preferably, the peptides have greater than 60% sequence similarity. Most preferably, the peptides have greater than 80% sequence similarity, e.g. 95% similarity. 
     Having characterised a gene according to the invention, it is possible to use the gene sequence to establish homologies in other microorganisms. In this way it is possible to determine whether other microorganisms have similar outer surface products. Sequence homologies may be established by searching in existing databases, e.g. EMBL or Genbank. 
     Peptides or proteins according to the invention may be purified and isolated by methods known in the art. In particular, having identified the gene sequence, it will be possible to use recombinant techniques to express the genes in a suitable host. Active fragments and homologues can be identified and may be useful in therapy. For example, the peptides or their active fragments may be used as antigenic determinants in a vaccine, to elicit an immune response. They may also be used in the preparation of antibodies, for passive immunisation, or diagnostic applications. Suitable antibodies include monoclonal antibodies, or fragments thereof, including single chain fv fragments. Methods for the preparation of antibodies will be apparent to those skilled in the art. 
     The preparation of vaccines based on attenuated microorganisms is known to those skilled in the art. Vaccine compositions can be formulated with suitable carriers or adjuvants, e.g. alum, as necessary or desired, and used in therapy, to provide effective immunisation against Group B Streptococci or other related microorganisms. The preparation of vaccine formulations will be apparent to the skilled person. 
     More generally, and as is well known to those skilled in the art, a suitable amount of an active component of the invention can be selected, for therapeutic use, as can suitable carriers or excipients, and routes of administration. These factors will be chosen or determined according to known criteria such as the nature/severity of the condition to be treated, the type or health of the subject etc. 
     The products of the present invention were identified as follows: 
     A partial gene library of GBS (strain M732) chromosomal DNA was prepared using the plasmid vectors pFW-phoA1, pFW-phoA2 and pFW-phoA3 (Podbielski, A. et al. 1996. Gene 177:137-147). These plasmids possess a constitutive spectinomycin adenyltransferase antibiotic resistance marker, which confers a high level of spectinomycin resistance and is therefore easily selected. Furthermore, these vectors contain a truncated (leaderless)  Escherichia coli  phoA gene for alkaline phosphatase. The three vectors differ only with respect to the reading frame in which the leaderless phoA gene exists, as compared to an upstream in-frame BamHI restriction enzyme site. Because this truncated  E. coli  phoA gene lacks the appropriate leader sequence for export of this enzyme across the bacterial membrane, extracellular alkaline phosphatase activity is absent when these plasmids are propagated in an  E. coli  phoA mutant (e.g. strain DH5α). The chromogenic alkaline phosphatase substrate, XP (5-bromo-4-chloro-3-indolyl-phosphate), does not enter intact bacterial cells and therefore only exported or surface associated alkaline phosphatase activity can be detected. When exported or surface associated alkaline phosphatase activity is present, the chromogenic XP substrate is cleaved to yield a blue pigment and the corresponding bacterial colonies can be identified by their blue colour. 
     Plasmid DNA was digested to completion with BamHI and dephosphorylated using shrimp alkaline phosphatase. GBS genomic DNA was partially digested with Sau3AI, siz fractionated on a sucrose gradient and fragments &lt;1 kb in size were ligated into the prepared pFW-phoA vectors.  E. coli  strain DH5α was chosen as the cloning host since it lacks a functional phoA gene. Recombinant plasmids were selected on Luria agar containing 100 μg/ml of spectinomycin and 40 μg/ml of the chromogenic XP substrate.  E. coli  transformants harbouring plasmids containing GBS insert DNA that complements the export signal sequence of the leaderless phoA gene were identified by the blue colour of the colonies. Approximately 30000 different recombinant plasmids containing GBS insert DNA were screened in this manner and 83 recombinant plasmids, which complemented the leaderless phoA, were chosen for further study. 
     From these experiments, several clones were selected each containing a plasmid containing a gene (or part thereof), which complemented the leaderless phoA. 
     Having identified the gene in each clone it is then possible to obtain the full-length gene sequence, as follows. 
     Using the identified and sequenced gene fragment, oligonucleotide primers were designed for genomic DNA sequencing. These primers were designed so as to sequence in an ‘outward’ direction from the obtained sequence. Once read, the sequence obtained was checked to see if the 5′ and 3′ termini of the gene had been reached. The presence of these features was identified by checking against homologous sequences, and for the 5′ end the presence of an AUG start codon (or accepted equivalent) preceded by a Shine-Dalgarno consensus sequence, and for the 3′ end, the presence of a translation termination (Stop) codon. 
     Upon identification of the full-length gene, primers were designed for amplification of full-length product. Primers used included restriction enzyme recognition sites (NcoI at the 5′end and Eco0109I at the 3′ end) to allow subsequent cloning of the product into the Lactococcal expression system used. 
     PCR was carried out using the primers, and the products cloned into a pCR 2.1 cloning vector (In Vitrogen). Following confirmation of the presence of the cloned fragment, the DNA was excised using the restriction enzymes NcoI and Eco0109I. 
     The vector into which this fragment was inserted was a modified version of pNZ8048 (Kuipers, O. P. et al. (1998) J. Biotech 64: 15-21). This vector, harbouring a lactococcal origin of replication, a chloramphenicol resistance marker, an inducible nisin promoter and a multicloning site was altered by the replacement of the multicloning site with two 10×His tags, flanked on the 5-most end with an NcoI site, split in the middle with a multicloning site (including an Eco0109I site), and a Stop (termination) codon at the 3′end of the His tags. 
     The gene of interest was inserted so that a 10×His tag was in the 3′ position relative to the coding region. Following transformation of the recombinant plasmid into  L. lactis  (strain NZ9000—Kuipers, O. P. et al. (1998)  supra ), a 400 ml liquid culture was set up and translation of the protein was induced by the addition of nisin to the culture. After a 2 hour incubation, the cells were harvested and lysed by bead beating. The resultant lysate was cleared by centrifugation, then passed over a metal affinity (Talon, Clonetech) column. The column was washed repeatedly before bound proteins were eluted with Imidazole. 
     To identify fractions containing the His-tagged recombinant protein, an aliquot from each fraction was analysed by SDS-PAGE, Western blotted and probed with anti-His antibodies. 
     The recombinant protein obtained was then used to immunise New Zealand white rabbits, with pre-immune sera being harvested prior to immunisation. Following a boost, the rabbits were sacrificed and sera collected. This sera was used in Western blots, ELISA and animal protection models. 
     Using the sera obtained from the animal studies, immunosorption studies were carried out. 
     Group B Streptococcus was grown in 20 ml Todd Hewitt broth (THB) for 8 hours, harvested and resuspended in 5 ml PBS. 50 μl aliquots of this were used to coat wells in a 96 well plate (Nunc Immuno-Sorb). This was left at 4° C. overnight to allow for adsorbance of the bacteria onto the plate. Plates were washed twice with PBS, then blocked with 3% BSA in PBS for 1 hr at 37° C. Plates were again washed. Serial 10 fold dilutions of the sera were made in PBS and 50 μl of these dilutions were added to the wells of the plate, in duplicate. The plate was covered and incubated for 1 hr at 37° C. The plate was washed, then 50μl anti-rabbit alkaline phosphatase conjugated secondary antibody at a concentration of 1:5000 was added to each well. Following incubation at 37° C. for an hour, the plate was washed again. 50 μl substrate (PNPP) was added to each well, and the reaction allowed to proceed for 30 min before the adsorbance was read at 405 nm. 
     Animal protection studies were also carried out to test the effectiveness of protection on the immunised rabbits. 
     GBS M732 was grown up in THB until mid-log phase was reached—approximately 5 hours. Cells were counted in a counting chamber, and bacteria were diluted to give a concentration of 2×10 7  bacteria per ml in pre-immune or test sera. 50 μl of this was injected via the intraperitoneal route into 0-1 day old mice. The mice were observed for survival over 48 hours. 
     The following Examples illustrate the invention. 
     EXAMPLE 1 
     A first clone contained a gene sequence identified herein as SEQ ID NO. 1, with an amino acid sequence identified as SEQ ID NO. 2, and classified as pho1-13. 
     A comparison of the amino acid sequence of pho1-13 was performed. 
     Homologues to the GBS pho1-13 gene product can be identified in  Streptococcus pyogenes, S. pneumoniae, S. salivarius, Escherichia coli, Yersinia enterocolitica, Aquifex aeolicus, Helicobacter pylon  and  Haemophilus influenzae . The  S. pyogenes  and  S. pneumoniae  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In all other cases, the above homologues can be identified as ATP-dependent Clp protease proteolytic subunits. The catalytic activity of Clp proteases results in the hydrolysis of proteins to small peptides in the presence of ATP and magnesium (Giffard, P. M. et al. 1993. J. Gen. Microbiol. 139:913-920). Furthermore, the ClpP component of Clp proteases has been shown to be induced as part of the heat shock response (Kroh, H. E. and L. D. Simon. 1990. J. Bacteriol. 172:6026-6034) and it is probable that this subunit or the complete proteolytic domain would associated with the bacterial surface. 
     Immunisation studies, carried out as described above, yielded the following results. 
     
       
         
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
                   
               
               
                   
                 No animals surviving 
                   
               
               
                   
                 at time (hrs) 
               
             
          
           
               
                   
                 Treatment 
                 No animals 
                 24 
                 48 
               
               
                   
                   
               
             
          
           
               
                   
                 PBS 
                 10 
                 7 
                 0 
               
               
                   
                 Pre-immunised 
                 37 
                 13 
                 0 
               
               
                   
                 Immunised 
                 38 
                 17 
                 9 
               
               
                   
                   
               
             
          
         
       
     
     EXAMPLE 2 
     A second clone was selected containing a plasmid designated pho1-14. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences are shown as SEQ ID NOS. 3 and 4, respectively. 
     A comparison of the amino acid sequence of pho1-14 was performed. 
     Homologues to the GBS pho1-14 gene product can be identified in  Streptococcus pyogenes, Enterococcus faecalis  and  Streptococcus pneumoniae . These homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. Additionally, two possible homologues were also identified from  Shigella flexneri  (SpaR) and  Yersinia pseudotuberculosis  (YscT). These latter two homologues are related proteins, believed to be anchored in the bacterial membrane (Bergman, T. et al. 1994. J. Bacteriol. 176:2619-2626). In  S. flexneri , the product of the spaR gene has been shown to be important for invasion of epithelial cells (Sasakawa, C. et al. 1993. J. Bacteriol. 175:2334-2346). Furthermore, the product of the spaR gene is also required for surface presentation of invasion plasmid antigens. The analogous protein in  Y. pseudotuberculosis  is a component of the Yop secretion system and is also important for virulence in this organism. 
     EXAMPLE 3 
     A third clone was selected containing a plasmid designated pho1-5. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. Th nucleotide and deduced amino acid sequences are shown as SEQ ID NOS. 5 and 6. 
     A comparison of the amino acid sequence of pho1-5 was performed. 
     Homologues to the GBS pho1-5 gene product can only be identified in  Streptococcus pyogenes  and  Staphylococcus carnosus  (sceA). The  S. pyogenes  homologue was identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. Furthermore, little information is available on the function of the sceA gene product from  S. carnosus . The sceA gene product shows some sequence similarity to the aggregation promoting protein from  Lactobacillus gasseri . Based on analysis of the sceA gene product, this molecule contains a well-conserved signal sequence and is apparently secreted or associated with the bacterial cell surface. 
     EXAMPLE 4 
     A further clone was selected containing a plasmid designated pho3-3. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences are shown as SEQ ID NOS. 7 and 8. 
     A comparison of the amino acid sequence of pho3-3 was performed. 
     Homologues to the GBS pho3-3 gene product can be identified in  Streptococcus mutans  (rmiC), (cpsM)  S. pneumoniae  and  S. pyogenes . The  S. pyogenes  homologue was identified from genome sequence data and no annotations were available as to the identity of the gene or gene product. In  S. pneumoniae , the homologue can be identified as dTDP-4-keto-6-deoxy glucose-3,5-epimerase. In the other two cases, the above homologues can be identified as dTDP-4-keto-L-rhamnose reductase (rmlC). In  S. mutants , the gene encoding this enzyme, rmlC, is part of the rml locus. The rml locus consists of three genes which exhibit significant similarity to enzymes involved in the biosynthesis of dTDP-rhamnose, the immediate precursor of the rhamnose component in the  S. mutans  polysaccharide capsule (Tsukioka, Y. et al. 1997. J. Bacteriol. 179:1126-1134). An analogous locus has also been identified in  S. pneumoniae  (Coffey, T. J. et al. 1998. Mol. Micobiol. 17:73-83). Almost all Streptococci characteristically possess rhamnose in their cell wall associated polysaccharides (Schleifer, K. H. and R. Kilper-Bälz. 1987. Syst. Appl. Microbiol. 10:1-19), and it is highly probable that dTDP-4-keto-L-rhamnose reductase would be associated with the outer surface in Streptococci. 
     EXAMPLE 5 
     A further clone was selected containing a plasmid designated pho2-10. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. 
     The nucleotide sequence is shown as SEQ ID NO. 9. From this, upstream and downstream coding regions were identified, and the deduced amino acid sequences shown as SEQ ID NOS. 10 and 11. 
     A comparison of the amino acid sequences of pho2-10 was performed. 
     Homologues to the CBS pho2-10 gene product can be identified in  Streptococcus pyogenes, Enterococcus faecalis, Debaryomyces occidentalis  (hatI) and  Escherichia coli  (trkD). The  S. pyogenes  and  E. faecalis  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In the yeast  D. occidentalis , the hak1 gene is a homologue of the trkD gene from  E. coli  (Banuelos, M. A. et al. 1995. EMBO J. 14:3021-3027). The trkD gene of  E. coli  is part of the kup potassium uptake system. The specific homolog identified here is the kup system potassium uptake protein. The kup system is a constitutive potassium uptake system in  E. coli . The kup system potassium uptake protein contains a highly hydrophobic N-terminus that is predicted to span the membrane at least 12 times. Kup is not homologous to other known membrane protein sequences. There is no indication of ATP binding, and it is proposed that the system is driven by a chemiosmotic gradient (Schleyer, M. &amp; E. P. Bakker, 1993. J. Bacteriol. 175:6925-6931). 
     EXAMPLE 6 
     A further clone was selected containing a plasmid designated pho2-15. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 12 and 13. 
     A comparison of the amino acid sequence of pho2-15 was performed. 
     Homologues to the CBS pho2-15 gene product can be identified in  Streptococcus pyogenes, Streptococcus pneumoniae, Enterococcus faecalis  and  Escherichia coli  (gatc and SgcC). The  S. pyogenes, S. pneumoniae  and  E. faecalis  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In  E. coli , the gatC and sgcC gene products can be identified as being the IIC component of phosphoenolypyruvate-dependent sugar phosphotransferase systems (PTS), a major carbohydrate active-transport system. In PTS systems, the IIC component is typically involved in binding of extracellular carbohydrates and forms a complex with the IID component to constitute a membrane channel (Nobelmann, B. and J. W. Lengeler. 1995. Biochim. Biophys. Acta 1262:69-72). 
     EXAMPLE 7 
     A further clone was selected containing a plasmid designated pho2-2. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 14 and 15, respectively. 
     A comparison of the amino acid sequence of pho2-2 was performed. 
     Homologues to the GBS pho2-2 gene product can be identified in  Enterococcus faecalis, Escherichia coli  (malK and afuC),  Bacillus subtilis  (glnO),  Haemophilus influenzae  (yebM and potA),  Streptococcus pyogenes, Streptococcus pneumoniae  and  Salmonella typhimurium  (malK). The  E. faecalis, S. pyogenes  and  S. pneumoniae  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In all other cases, homologues represented ATP-binding transport proteins that are part of ABC type transporters. Many of the components of ABC type transporters are membrane or cell surface associated, as these systems are involved in the transport of macromolecules from the extracellular environment to the intracellular compartment. 
     EXAMPLE 8 
     A further clone was selected containing a plasmid designated pho3-14. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 16 and 17. 
     A comparison of the amino acid sequence of pho3-14 was performed and no homologues could be identified in any of the public databases. One homologue to the CBS pho3-14 gene product can be identified in  Streptococcus pyogenes , but this homologue was identified from genome sequence data and no annotations were available as to the identity of th gene or gene product. Using this  S. pyogenes  homologue to search the public databases yielded no further information. Since the pho3-14 product complemented the leaderless phoA gene, it can be concluded that this protein (or part thereof) would most probably be located extracellularly. 
     EXAMPLE 9 
     A further clone was selected containing a plasmid designated pho3-17. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 18 and 19. 
     A comparison of the amino acid sequence of pho3-17 was performed. 
     Homologues to the GBS Pho3-17 gene product can be identified in  Streptococcus mutans  and  Lactococcus lactis , with similarity being shown to N-acetyl muramidase. Similarity is also seen with an unidentified gene, yubE from  Bacillus subtilis.    
     N-acetylmuramidase is an autolysin that is involved in cell division. Using this limited information along with the fact that pho3-17 complemented the leaderless phoA gene, it can be concluded that the pho3-17 product would most probably be located extracellularly. 
     EXAMPLE 10 
     A further clone was selected containing a plasmid designated pho3-18. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 20 and 21. 
     A comparison of the amino acid sequence of pho3-18 was performed. 
     Homologues to the CBS pho3-18 gene product can be identified in  Streptococcus pyogenes  and  Streptococcus pneumoniae . These homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. Using these  S. pyogenes  and  S. pneumoniae  homologues to search the public databases showed some similarity to outer surface and membrane spanning proteins. Since the ORF3-18 product complemented the leaderless phoA gene, it can be concluded that this protein (or part thereof) would most probably be located extracellularly. 
     EXAMPLE 11 
     A further clone was selected containing a plasmid designated pho3-1. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 22 and 23. 
     A comparison of the amino acid sequence of pho3-1 was performed. 
     Homologues to the GBS pho3-1 gene product can be identified in  Streptococcus pyogenes, Streptococcus pneumoniae, Bacillus subtilis  (yutD) and  Enterococcus faecalis . The  S. pyogenes, S. pneumoniae  and  E. faecalis  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In  B. subtilis , the function of the yutD gene product is unknown. It can be noted however, that the yutD gene is located on the  B. subtilis  chromosome in a region containing genes involved in cell wall synthesis. The fact that this DNA sequence complemented the leaderless phoA gene suggests that this gene product is extracellularly located. 
     EXAMPLE 12 
     A further clone was selected containing a plasmid designated pho3-21. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 24 and 25. 
     A comparison of the amino acid sequence of pho3-21 was performed. 
     Homologues to the GBS pho3-21 gene product can be identified in  Streptococcus pyogenes, Streptococcus pneumoniae, Lactobacillus fermentum  (bspA) and  Lactobacillus reuteri  (cnb). The  S. pyogenes  and  S. pneumoniae  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In  L. fermentum , the bspA gene product has been identified as being a basic cell surface-located protein that has some sequence similarity to family III of the bacterial solute-binding proteins (Turner, M. S. et al. 1997. J. Bacteriol. 179:3310-3316). In  L. reuteri , the cnb gene product has been identified as a collagen binding protein that has some sequence similarity to the solute-binding component of bacterial ABC transporters (Roos, S. et al. 1996. FEMS Microbiol. Lett. 144:33-38). 
     EXAMPLE 13 
     A further clone was selected containing a plasmid designated pho3-22. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 26 and 27. 
     A comparison of the amino acid sequence of pho3-22 was performed. 
     Homologues to the GBS pho3-22 gene product can be identified in  Enterococcus faecalis, Streptococcus equisimilis  (lppC),  Pseudomonas fluorescens  (oprI) and  Streptococcus thermophilus  (orf142). The  E. faecalis  homolog was identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In  S. equisimilis , the lppC gene product has been identified as being a lipoprotein that is homologous to the E(P4) outer membrane protein from  Haemophilus influenzae  (Gase, K. et al. 1997. Med. Microbiol. Immunol. 186:63-73). Likewise, the P. fluorescens oprI gene encodes a major outer membrane lipoprotein (Cornelis, P. et al. 1989. Mol. Microbiol. 3:421-428). In  S. thermophilus , the orf142 product has been putatively identified as a cell surface exposed lipoprotein that may act as a receptor for the bacteriophages TP-J34 and Sfi21 (Neve, H. et al. 1998. Virology 241:61-72). The ORF3-22 product showed good similarity to the above homologues, particularly at the N-terminus. This is most likely the region required for complementation of the leaderless phoA gene, and therefore serves as a leader sequence. 
     EXAMPLE 14 
     A further clone was selected containing a plasmid designated pho3-23. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the genes are shown as SEQ ID NOS. 28 and 29. 
     A comparison of the amino acid sequence of pho3-23 was performed. 
     Homologues to the GBS pho3-23 gene product can be identified in  Streptococcus pyogenes, Streptococcus pneumoniae, Enterococcus faecalis  and  Streptococcus mutans  (perM). The  S. pyogenes, S. pneumoniae  and  E. faecalis  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In  S. mutans , the perM gene product has been presumptively identified as a permease, but no other information is available as to the function of this protein. Considering that the pho3-23 coding region complements the leaderless phoA gene, it can be concluded that the pho3-17 gene product would most probably be located extracellularly. 
     EXAMPLE 15 
     A further clone was selected containing a plasmid designated pho3-24. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 30 and 31. 
     A comparison of the amino acid sequence of pho3-24 was performed. 
     Homologues to the GBS pho3-24 gene product can be identified in  Streptococcus mutans  (dltB),  Streptococcus pneumoniae, Streptococcus pyogenes, Enterococcus faecalis, Lactobacillus casei  (dltB) and  Bacillus subtilis  (dltB). The  S. pneumoniae, S. pyogenes  and  E. faecalis  homologues were identified from genome sequence data and no annotations were available as to the identity of the gene or gene products. In  S. mutans, L. casei  and  B. subtilis , the dltB gene product has been identified as being a basic membrane protein that is involved in the transport of activated D-alanine through the cell membrane. The dltB gene product is involved in the biosynthesis of D-alanyl-lipoteichoic acid (Heaton, M. P. and F. C. Neuhaus. 1992. J. Bacteriol. 174:4707-4717). In  L. casei  and  B. subtilis , the dltB gene product is believed to contain at least 9 membrane spanning domains, indicating that the protein or portions thereof are exposed to the outside of the cell. 
     EXAMPLE 16 
     A further clone was selected containing a plasmid designated pho3-29. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 32 and 33. 
     A comparison of the amino acid sequence of pho3-29 was performed. 
     Homologues to the GBS pho3-29 gene product can be identified in  Borrelia burgdorferi  (p23 or ospC),  Bacillus brevis  (owp) and  Pseudomonas aeruginosa  (oprI). Although these homologues are not related to each other, they all represent major outer surface proteins. In  B. burgdorferi , the ospC gene product has been identified as being a 23-kDa protein that is the immunodominant antigen on the surface of this bacterium (Padula, S. J. et al. 1993. Infect. Immun. 61:5097-5105). The owp gene product from  B. brevis  is one of two major cell wall proteins involved in the surface layer lattice (Tsuboi, A. 1988. J. Bacteriol. 170:935-945). Finally, the oprI gene from  P. aeruginosa  encodes a major outer membrane lipoprotein precursor (Saint-Onge, A. et al. 1992. J. Gen. Microbiol. 138:733-741). 
     EXAMPLE 17 
     A further clone was selected containing a plasmid designated pho3-50. This plasmid contained a gene (or part thereof), which complemented the leaderless phoA. The nucleotide and deduced amino acid sequences of the gene are shown as SEQ ID NOS. 34 and 35. 
     A comparison of the amino acid sequence of pho3-50 was performed. 
     Homologues to the GBS pho3-50 gene product can be identified in a variety of Streptococci (penA, pbp2B, pbpB2),  Borrelia burgdorferi  (pbp2),  Enterococcus faecalis  (pbpC),  Staphylococcus aureus  (pbpA),  Mycobacterium leprae  (pbpB) and  Helicobacter pylori  (pbp2). In all cases, the above homologues can be identified as penicillin binding proteins (PBPs). Genes encoding penicillin binding proteins are often located in a cluster of genes associated with cell wall synthesis (Pucci, M. J. et al. 1997. J. Bacteriol. 179:5632-5635). Furthermore, PBPS are typically integrated into the cell wall of a bacterium with some or all of the protein being exposed on the outer bacterial surface. 
     
       
         
           
             35 
           
           
             1 
             587 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(582) 
                 
             
           
            1
atg atc cca gta gta atc gaa caa aca agt cgt ggt gaa cgt tct tat       48
Met Ile Pro Val Val Ile Glu Gln Thr Ser Arg Gly Glu Arg Ser Tyr
1               5                   10                  15
gat att tac tca cgt ctt tta aaa gat cgt att att atg ttg aca ggc       96
Asp Ile Tyr Ser Arg Leu Leu Lys Asp Arg Ile Ile Met Leu Thr Gly
            20                  25                  30
caa gtt gag gat aat atg gcc aat agt atc att gca cag tta ttg ttt      144
Gln Val Glu Asp Asn Met Ala Asn Ser Ile Ile Ala Gln Leu Leu Phe
        35                  40                  45
ctc gat gca caa gat aat aca aag gat att tac ctt tat gtc aat aca      192
Leu Asp Ala Gln Asp Asn Thr Lys Asp Ile Tyr Leu Tyr Val Asn Thr
    50                  55                  60
cca ggt ggt tca gta tcg gct gga ctt gct att gtg gac acc atg aac      240
Pro Gly Gly Ser Val Ser Ala Gly Leu Ala Ile Val Asp Thr Met Asn
65                  70                  75                  80
ttc att aaa tcg gac gta cag acg att gtt atg ggg atg gct gct tcg      288
Phe Ile Lys Ser Asp Val Gln Thr Ile Val Met Gly Met Ala Ala Ser
                85                  90                  95
atg gga acc att att gct tca agt ggt gct aaa gga aaa cgt ttt atg      336
Met Gly Thr Ile Ile Ala Ser Ser Gly Ala Lys Gly Lys Arg Phe Met
            100                 105                 110
tta ccg aat gca gaa tat atg atc cac caa cca atg ggc gga aca ggc      384
Leu Pro Asn Ala Glu Tyr Met Ile His Gln Pro Met Gly Gly Thr Gly
        115                 120                 125
gga ggt aca cag caa tct gat atg gct atc gct gct gag cat ctt tta      432
Gly Gly Thr Gln Gln Ser Asp Met Ala Ile Ala Ala Glu His Leu Leu
    130                 135                 140
aaa acg cgt cat act tta gaa aaa atc tta gct gat aat tct ggt caa      480
Lys Thr Arg His Thr Leu Glu Lys Ile Leu Ala Asp Asn Ser Gly Gln
145                 150                 155                 160
tct att gaa aaa gtc cat gat gat gca gag cgt gat cgt tgg atg agt      528
Ser Ile Glu Lys Val His Asp Asp Ala Glu Arg Asp Arg Trp Met Ser
                165                 170                 175
gct caa gaa aca ctt gat tat ggc ttt att gat gaa atc atg gct aat      576
Ala Gln Glu Thr Leu Asp Tyr Gly Phe Ile Asp Glu Ile Met Ala Asn
            180                 185                 190
aat gaa taagg                                                        587
Asn Glu
 
           
             2 
             194 
             PRT 
             Streptococcus agalactiae 
           
            2
Met Ile Pro Val Val Ile Glu Gln Thr Ser Arg Gly Glu Arg Ser Tyr
1               5                   10                  15
Asp Ile Tyr Ser Arg Leu Leu Lys Asp Arg Ile Ile Met Leu Thr Gly
            20                  25                  30
Gln Val Glu Asp Asn Met Ala Asn Ser Ile Ile Ala Gln Leu Leu Phe
        35                  40                  45
Leu Asp Ala Gln Asp Asn Thr Lys Asp Ile Tyr Leu Tyr Val Asn Thr
    50                  55                  60
Pro Gly Gly Ser Val Ser Ala Gly Leu Ala Ile Val Asp Thr Met Asn
65                  70                  75                  80
Phe Ile Lys Ser Asp Val Gln Thr Ile Val Met Gly Met Ala Ala Ser
                85                  90                  95
Met Gly Thr Ile Ile Ala Ser Ser Gly Ala Lys Gly Lys Arg Phe Met
            100                 105                 110
Leu Pro Asn Ala Glu Tyr Met Ile His Gln Pro Met Gly Gly Thr Gly
        115                 120                 125
Gly Gly Thr Gln Gln Ser Asp Met Ala Ile Ala Ala Glu His Leu Leu
    130                 135                 140
Lys Thr Arg His Thr Leu Glu Lys Ile Leu Ala Asp Asn Ser Gly Gln
145                 150                 155                 160
Ser Ile Glu Lys Val His Asp Asp Ala Glu Arg Asp Arg Trp Met Ser
                165                 170                 175
Ala Gln Glu Thr Leu Asp Tyr Gly Phe Ile Asp Glu Ile Met Ala Asn
            180                 185                 190
Asn Glu
 
           
             3 
             218 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(216) 
                 
             
           
            3
atc aga gca tat tct ggt cct ctt tcg gtt ttc ctg cca cgt ttt aaa       48
Ile Arg Ala Tyr Ser Gly Pro Leu Ser Val Phe Leu Pro Arg Phe Lys
1               5                   10                  15
gct tgt gat ata ata gtc aat gtg agg agg act atc atg tta ttt aag       96
Ala Cys Asp Ile Ile Val Asn Val Arg Arg Thr Ile Met Leu Phe Lys
            20                  25                  30
gaa aaa att cct gga cta ata tta tgc ttt att att gct ata cca tct      144
Glu Lys Ile Pro Gly Leu Ile Leu Cys Phe Ile Ile Ala Ile Pro Ser
        35                  40                  45
tgg ttg ctt ggg ctt tat ctc cct tta ata gga gca cca gtc ttt gct      192
Trp Leu Leu Gly Leu Tyr Leu Pro Leu Ile Gly Ala Pro Val Phe Ala
    50                  55                  60
atc ttg att gga ata att gtt gga tc                                   218
Ile Leu Ile Gly Ile Ile Val Gly
65                  70
 
           
             4 
             72 
             PRT 
             Streptococcus agalactiae 
           
            4
Ile Arg Ala Tyr Ser Gly Pro Leu Ser Val Phe Leu Pro Arg Phe Lys
1               5                   10                  15
Ala Cys Asp Ile Ile Val Asn Val Arg Arg Thr Ile Met Leu Phe Lys
            20                  25                  30
Glu Lys Ile Pro Gly Leu Ile Leu Cys Phe Ile Ile Ala Ile Pro Ser
        35                  40                  45
Trp Leu Leu Gly Leu Tyr Leu Pro Leu Ile Gly Ala Pro Val Phe Ala
    50                  55                  60
Ile Leu Ile Gly Ile Ile Val Gly
65                  70
 
           
             5 
             705 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(705) 
                 
             
           
            5
atg aat aaa aga aga aaa tta tca aaa ttg aat gta aaa aar caa cat       48
Met Asn Lys Arg Arg Lys Leu Ser Lys Leu Asn Val Lys Lys Gln His
1               5                   10                  15
tta gct tat gga gct atc act tta gta gcc ctt ttt tca tgt att ttg       96
Leu Ala Tyr Gly Ala Ile Thr Leu Val Ala Leu Phe Ser Cys Ile Leu
            20                  25                  30
gct gta acg gtc atc ttt aaa agt tca caa gtt act act gaa tct ttg      144
Ala Val Thr Val Ile Phe Lys Ser Ser Gln Val Thr Thr Glu Ser Leu
        35                  40                  45
tca aaa gca gat aaa gtt cgc gta gcc aaa aaa tca aaa atg act aag      192
Ser Lys Ala Asp Lys Val Arg Val Ala Lys Lys Ser Lys Met Thr Lys
    50                  55                  60
gcg aca tct aaa tca aaa gta gaa gat gta aaa cag gct cca aaa cct      240
Ala Thr Ser Lys Ser Lys Val Glu Asp Val Lys Gln Ala Pro Lys Pro
65                  70                  75                  80
tct cag gca tct aat gaa gcc cca aaa tca agt tct caa tct aca gaa      288
Ser Gln Ala Ser Asn Glu Ala Pro Lys Ser Ser Ser Gln Ser Thr Glu
                85                  90                  95
gct aat tct cag caa caa gtt act gcg agt gaa gag acg gct gta gaa      336
Ala Asn Ser Gln Gln Gln Val Thr Ala Ser Glu Glu Thr Ala Val Glu
            100                 105                 110
caa gca gtt gta aca gaa ata ccc ctg cta cca gtc agg cac aac aac      384
Gln Ala Val Val Thr Glu Ile Pro Leu Leu Pro Val Arg His Asn Asn
        115                 120                 125
ctt tat gct gtt act gag aca cct tac aac cct gct caa cca cca gac      432
Leu Tyr Ala Val Thr Glu Thr Pro Tyr Asn Pro Ala Gln Pro Pro Asp
    130                 135                 140
caa gtg gcc agg tat gag caa tgg aaa tac tgc cag gcg gtc gga tct      480
Gln Val Ala Arg Tyr Glu Gln Trp Lys Tyr Cys Gln Ala Val Gly Ser
145                 150                 155                 160
gct gct gca gca caa atg gct gct gca aca gga gtc cct cag tct act      528
Ala Ala Ala Ala Gln Met Ala Ala Ala Thr Gly Val Pro Gln Ser Thr
                165                 170                 175
tgg gaa cat att att gcc cgt gaa tca aat ggt aat cct aat gtt gct      576
Trp Glu His Ile Ile Ala Arg Glu Ser Asn Gly Asn Pro Asn Val Ala
            180                 185                 190
aat gcc tca gga gct tca gga ctt ttc caa acg atg cca ggt tgg ggt      624
Asn Ala Ser Gly Ala Ser Gly Leu Phe Gln Thr Met Pro Gly Trp Gly
        195                 200                 205
tca aca gct aca gtt cag gat caa gta att cag cta tta aag ctt att      672
Ser Thr Ala Thr Val Gln Asp Gln Val Ile Gln Leu Leu Lys Leu Ile
    210                 215                 220
cgt gct caa ggg tta tca gct ggg tac cag tga                          705
Arg Ala Gln Gly Leu Ser Ala Gly Tyr Gln
225                 230
 
           
             6 
             234 
             PRT 
             Streptococcus agalactiae 
           
            6
Met Asn Lys Arg Arg Lys Leu Ser Lys Leu Asn Val Lys Lys Gln His
1               5                   10                  15
Leu Ala Tyr Gly Ala Ile Thr Leu Val Ala Leu Phe Ser Cys Ile Leu
            20                  25                  30
Ala Val Thr Val Ile Phe Lys Ser Ser Gln Val Thr Thr Glu Ser Leu
        35                  40                  45
Ser Lys Ala Asp Lys Val Arg Val Ala Lys Lys Ser Lys Met Thr Lys
    50                  55                  60
Ala Thr Ser Lys Ser Lys Val Glu Asp Val Lys Gln Ala Pro Lys Pro
65                  70                  75                  80
Ser Gln Ala Ser Asn Glu Ala Pro Lys Ser Ser Ser Gln Ser Thr Glu
                85                  90                  95
Ala Asn Ser Gln Gln Gln Val Thr Ala Ser Glu Glu Thr Ala Val Glu
            100                 105                 110
Gln Ala Val Val Thr Glu Ile Pro Leu Leu Pro Val Arg His Asn Asn
        115                 120                 125
Leu Tyr Ala Val Thr Glu Thr Pro Tyr Asn Pro Ala Gln Pro Pro Asp
    130                 135                 140
Gln Val Ala Arg Tyr Glu Gln Trp Lys Tyr Cys Gln Ala Val Gly Ser
145                 150                 155                 160
Ala Ala Ala Ala Gln Met Ala Ala Ala Thr Gly Val Pro Gln Ser Thr
                165                 170                 175
Trp Glu His Ile Ile Ala Arg Glu Ser Asn Gly Asn Pro Asn Val Ala
            180                 185                 190
Asn Ala Ser Gly Ala Ser Gly Leu Phe Gln Thr Met Pro Gly Trp Gly
        195                 200                 205
Ser Thr Ala Thr Val Gln Asp Gln Val Ile Gln Leu Leu Lys Leu Ile
    210                 215                 220
Arg Ala Gln Gly Leu Ser Ala Gly Tyr Gln
225                 230
 
           
             7 
             594 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(594) 
                 
             
           
            7
atg act gaa cca ttt ttt gat aaa gaa tta act tgt cgc cca att gaa       48
Met Thr Glu Pro Phe Phe Asp Lys Glu Leu Thr Cys Arg Pro Ile Glu
1               5                   10                  15
gcc att cct gaa ttg ttg gaa ttc gat att acc gtt cgt gga gac aac       96
Ala Ile Pro Glu Leu Leu Glu Phe Asp Ile Thr Val Arg Gly Asp Asn
            20                  25                  30
cgt gga tgg ttc aaa gag aac ttt caa aaa gaa aaa atg ata ccg ctt      144
Arg Gly Trp Phe Lys Glu Asn Phe Gln Lys Glu Lys Met Ile Pro Leu
        35                  40                  45
ggt ttc cca gaa agc ttc ttt gag gca gac aaa cta caa aat aat att      192
Gly Phe Pro Glu Ser Phe Phe Glu Ala Asp Lys Leu Gln Asn Asn Ile
    50                  55                  60
tcg ttt aca aaa aaa aat act ttg cga ggt ctc cat gca gag cct tgg      240
Ser Phe Thr Lys Lys Asn Thr Leu Arg Gly Leu His Ala Glu Pro Trp
65                  70                  75                  80
gat aaa tat gtt tcg atc gct gat gaa gga cgt gtg atc ggt act tgg      288
Asp Lys Tyr Val Ser Ile Ala Asp Glu Gly Arg Val Ile Gly Thr Trp
                85                  90                  95
gtt gac ctc cgt gaa ggt gac agt ttt ggt aac gtt tac caa acg att      336
Val Asp Leu Arg Glu Gly Asp Ser Phe Gly Asn Val Tyr Gln Thr Ile
            100                 105                 110
atc gat gcc tca aaa ggt att ttt gtt cca cgc ggc gtt gct aat ggt      384
Ile Asp Ala Ser Lys Gly Ile Phe Val Pro Arg Gly Val Ala Asn Gly
        115                 120                 125
ttc caa gtt ctt tca gat aaa gca gct tat act tat ctc gtt aac gat      432
Phe Gln Val Leu Ser Asp Lys Ala Ala Tyr Thr Tyr Leu Val Asn Asp
    130                 135                 140
tat tgg gca ctt gaa ctc aaa cca aaa tat gct ttc gtt aac tat gca      480
Tyr Trp Ala Leu Glu Leu Lys Pro Lys Tyr Ala Phe Val Asn Tyr Ala
145                 150                 155                 160
gat cca aat cta ggc att cag tgg gaa aat ctw gaa gaa gca gaa gtc      528
Asp Pro Asn Leu Gly Ile Gln Trp Glu Asn Leu Glu Glu Ala Glu Val
                165                 170                 175
tca gaa gca gat aag aat cac cca ctt ctc aaa gat gta aaa cct ttg      576
Ser Glu Ala Asp Lys Asn His Pro Leu Leu Lys Asp Val Lys Pro Leu
            180                 185                 190
aag aag gaa gat ttg taa                                              594
Lys Lys Glu Asp Leu
        195
 
           
             8 
             197 
             PRT 
             Streptococcus agalactiae 
           
            8
Met Thr Glu Pro Phe Phe Asp Lys Glu Leu Thr Cys Arg Pro Ile Glu
1               5                   10                  15
Ala Ile Pro Glu Leu Leu Glu Phe Asp Ile Thr Val Arg Gly Asp Asn
            20                  25                  30
Arg Gly Trp Phe Lys Glu Asn Phe Gln Lys Glu Lys Met Ile Pro Leu
        35                  40                  45
Gly Phe Pro Glu Ser Phe Phe Glu Ala Asp Lys Leu Gln Asn Asn Ile
    50                  55                  60
Ser Phe Thr Lys Lys Asn Thr Leu Arg Gly Leu His Ala Glu Pro Trp
65                  70                  75                  80
Asp Lys Tyr Val Ser Ile Ala Asp Glu Gly Arg Val Ile Gly Thr Trp
                85                  90                  95
Val Asp Leu Arg Glu Gly Asp Ser Phe Gly Asn Val Tyr Gln Thr Ile
            100                 105                 110
Ile Asp Ala Ser Lys Gly Ile Phe Val Pro Arg Gly Val Ala Asn Gly
        115                 120                 125
Phe Gln Val Leu Ser Asp Lys Ala Ala Tyr Thr Tyr Leu Val Asn Asp
    130                 135                 140
Tyr Trp Ala Leu Glu Leu Lys Pro Lys Tyr Ala Phe Val Asn Tyr Ala
145                 150                 155                 160
Asp Pro Asn Leu Gly Ile Gln Trp Glu Asn Leu Glu Glu Ala Glu Val
                165                 170                 175
Ser Glu Ala Asp Lys Asn His Pro Leu Leu Lys Asp Val Lys Pro Leu
            180                 185                 190
Lys Lys Glu Asp Leu
        195
 
           
             9 
             1217 
             DNA 
             Streptococcus agalactiae 
             
               misc_feature 
               (571)..(571) 
               N can be A, G, C, or T. 
             
           
            9
tat tat tta atc gga ggg ttg gca gaa atg caa cat gtc aat cat tct       48
Tyr Tyr Leu Ile Gly Gly Leu Ala Glu Met Gln His Val Asn His Ser
1               5                   10                  15
tct ttt gat aaa gca tca aaa gca gga ttt att att gct tta ggc att       96
Ser Phe Asp Lys Ala Ser Lys Ala Gly Phe Ile Ile Ala Leu Gly Ile
            20                  25                  30
gtt tat gga gat att ggt aca agc cca ctc tat acg atg caa tca ttg      144
Val Tyr Gly Asp Ile Gly Thr Ser Pro Leu Tyr Thr Met Gln Ser Leu
        35                  40                  45
gtt gaa aac caa ggt ggt att tct agt gtc aca gaa tcg ttt atc tta      192
Val Glu Asn Gln Gly Gly Ile Ser Ser Val Thr Glu Ser Phe Ile Leu
    50                  55                  60
ggt tct ata tct tta atc ata tgg acc ttg aca ctt att aca act atc      240
Gly Ser Ile Ser Leu Ile Ile Trp Thr Leu Thr Leu Ile Thr Thr Ile
65                  70                  75                  80
aag tat gtg ctt gta gct tta aag gcg gat aat cac cac gaa ggt ggt      288
Lys Tyr Val Leu Val Ala Leu Lys Ala Asp Asn His His Glu Gly Gly
                85                  90                  95
att ttt tct tta tat acc ctt gtt aga aaa atg aca cct tgg tta att      336
Ile Phe Ser Leu Tyr Thr Leu Val Arg Lys Met Thr Pro Trp Leu Ile
            100                 105                 110
gtt ccg gct gtt att gga ggt gca acc ttg ttg tca gat gga gct ttg      384
Val Pro Ala Val Ile Gly Gly Ala Thr Leu Leu Ser Asp Gly Ala Leu
        115                 120                 125
acg cca gct gta acc gta ctt cag ccg tta agg att aaa gta gtt cct      432
Thr Pro Ala Val Thr Val Leu Gln Pro Leu Arg Ile Lys Val Val Pro
    130                 135                 140
agt ttg cag cat att tcc aga atc aga gta tgt tat ttt gcg acc ttg      480
Ser Leu Gln His Ile Ser Arg Ile Arg Val Cys Tyr Phe Ala Thr Leu
145                 150                 155                 160
tta ttt act gtt act ttt gcc atc caa ggt ttg gaa cgg gtg tta ttg      528
Leu Phe Thr Val Thr Phe Ala Ile Gln Gly Leu Glu Arg Val Leu Leu
                165                 170                 175
gaa tta ttg gcc att atg tta tat ggt ttg cct ttt ggt tta              570
Glu Leu Leu Ala Ile Met Leu Tyr Gly Leu Pro Phe Gly Leu
            180                 185                 190
ncggtctcct tatagttttg cccatccaga agttttcaag cattaatcca tactacggtt    630
tgaaattgtt atttagtcca gagaatcata aaggtatttt tattttag gat cta ttt     687
                                                     Asp Leu Phe
tcc tgg cga caa acg gga gca gaa gca cta tac tct gac tta ggt cat      735
Ser Trp Arg Gln Thr Gly Ala Glu Ala Leu Tyr Ser Asp Leu Gly His
    195                 200                 205
gtt ggg cgt gga aat ata cat gtt tca tgg ccg ttc gtt aag gtt gcc      783
Val Gly Arg Gly Asn Ile His Val Ser Trp Pro Phe Val Lys Val Ala
210                 215                 220                 225
att ata ctt tct tat tgt ggg caa ggg gca tgg att tta gct aat aag      831
Ile Ile Leu Ser Tyr Cys Gly Gln Gly Ala Trp Ile Leu Ala Asn Lys
                230                 235                 240
aac gca gga aat gaa ttg aat ccc ttt ttt gct agt att cct tcg caa      879
Asn Ala Gly Asn Glu Leu Asn Pro Phe Phe Ala Ser Ile Pro Ser Gln
            245                 250                 255
ttt aca atg cat gtc gtt att tta gct act ttg gca gct atc atc gct      927
Phe Thr Met His Val Val Ile Leu Ala Thr Leu Ala Ala Ile Ile Ala
        260                 265                 270
tca cag gca ctg att tct ggatcaattt accttaagtt ctgagctatg             975
Ser Gln Ala Leu Ile Ser
    275
cgactaaaaa tattcccaca atttcgttca acttatcctg ttgacaatat tgggtcaaac   1035
ctacatacct ggtattaatt ggttcttatt tgccattaca acctctattg gtttgctttt   1095
taagacttca gcgcacatgg aagcagcata tggattagcg ataacaatta cgatgctaat   1155
gacaactatt ttactgtctt tctttttaat tcaaaaagga gtaaagagag gttttagcta   1215
tt                                                                  1217
 
           
             10 
             190 
             PRT 
             Streptococcus agalactiae 
           
            10
Tyr Tyr Leu Ile Gly Gly Leu Ala Glu Met Gln His Val Asn His Ser
1               5                   10                  15
Ser Phe Asp Lys Ala Ser Lys Ala Gly Phe Ile Ile Ala Leu Gly Ile
            20                  25                  30
Val Tyr Gly Asp Ile Gly Thr Ser Pro Leu Tyr Thr Met Gln Ser Leu
        35                  40                  45
Val Glu Asn Gln Gly Gly Ile Ser Ser Val Thr Glu Ser Phe Ile Leu
    50                  55                  60
Gly Ser Ile Ser Leu Ile Ile Trp Thr Leu Thr Leu Ile Thr Thr Ile
65                  70                  75                  80
Lys Tyr Val Leu Val Ala Leu Lys Ala Asp Asn His His Glu Gly Gly
                85                  90                  95
Ile Phe Ser Leu Tyr Thr Leu Val Arg Lys Met Thr Pro Trp Leu Ile
            100                 105                 110
Val Pro Ala Val Ile Gly Gly Ala Thr Leu Leu Ser Asp Gly Ala Leu
        115                 120                 125
Thr Pro Ala Val Thr Val Leu Gln Pro Leu Arg Ile Lys Val Val Pro
    130                 135                 140
Ser Leu Gln His Ile Ser Arg Ile Arg Val Cys Tyr Phe Ala Thr Leu
145                 150                 155                 160
Leu Phe Thr Val Thr Phe Ala Ile Gln Gly Leu Glu Arg Val Leu Leu
                165                 170                 175
Glu Leu Leu Ala Ile Met Leu Tyr Gly Leu Pro Phe Gly Leu
            180                 185                 190
 
           
             11 
             89 
             PRT 
             Streptococcus agalactiae 
           
            11
Asp Leu Phe Ser Trp Arg Gln Thr Gly Ala Glu Ala Leu Tyr Ser Asp
1               5                   10                  15
Leu Gly His Val Gly Arg Gly Asn Ile His Val Ser Trp Pro Phe Val
            20                  25                  30
Lys Val Ala Ile Ile Leu Ser Tyr Cys Gly Gln Gly Ala Trp Ile Leu
        35                  40                  45
Ala Asn Lys Asn Ala Gly Asn Glu Leu Asn Pro Phe Phe Ala Ser Ile
    50                  55                  60
Pro Ser Gln Phe Thr Met His Val Val Ile Leu Ala Thr Leu Ala Ala
65                  70                  75                  80
Ile Ile Ala Ser Gln Ala Leu Ile Ser
                85
 
           
             12 
             378 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(378) 
                 
             
           
            12
atg cag gta ttt tta aat att gtc aat aaa ttc ttt gat cca gtt att       48
Met Gln Val Phe Leu Asn Ile Val Asn Lys Phe Phe Asp Pro Val Ile
1               5                   10                  15
cat atg ggt tcg gga gtt gtg atg cta att gtc atg aca ggt tta gcc       96
His Met Gly Ser Gly Val Val Met Leu Ile Val Met Thr Gly Leu Ala
            20                  25                  30
atg ata ttt gga gtg aag ttt tct aaa gca ctt gaa ggt ggt att aag      144
Met Ile Phe Gly Val Lys Phe Ser Lys Ala Leu Glu Gly Gly Ile Lys
        35                  40                  45
tta gct att gct ctt acg ggt att ggt gct att att ggt att tta act      192
Leu Ala Ile Ala Leu Thr Gly Ile Gly Ala Ile Ile Gly Ile Leu Thr
    50                  55                  60
ggt gct ttt tcc gaa tca ctt caa gct ttt gtt aaa aat aca gga atc      240
Gly Ala Phe Ser Glu Ser Leu Gln Ala Phe Val Lys Asn Thr Gly Ile
65                  70                  75                  80
aat cta agc att att gac gtt ggt tgg gct cca tta gca act att aca      288
Asn Leu Ser Ile Ile Asp Val Gly Trp Ala Pro Leu Ala Thr Ile Thr
                85                  90                  95
tgg gga tca cca tat acg ctt tac ttc tta tta atc atg ctt att gtc      336
Trp Gly Ser Pro Tyr Thr Leu Tyr Phe Leu Leu Ile Met Leu Ile Val
            100                 105                 110
aat att gtt atg att gtt atg aaa aaa aaa cgg ata cct tag              378
Asn Ile Val Met Ile Val Met Lys Lys Lys Arg Ile Pro
        115                 120                 125
 
           
             13 
             125 
             PRT 
             Streptococcus agalactiae 
           
            13
Met Gln Val Phe Leu Asn Ile Val Asn Lys Phe Phe Asp Pro Val Ile
1               5                   10                  15
His Met Gly Ser Gly Val Val Met Leu Ile Val Met Thr Gly Leu Ala
            20                  25                  30
Met Ile Phe Gly Val Lys Phe Ser Lys Ala Leu Glu Gly Gly Ile Lys
        35                  40                  45
Leu Ala Ile Ala Leu Thr Gly Ile Gly Ala Ile Ile Gly Ile Leu Thr
    50                  55                  60
Gly Ala Phe Ser Glu Ser Leu Gln Ala Phe Val Lys Asn Thr Gly Ile
65                  70                  75                  80
Asn Leu Ser Ile Ile Asp Val Gly Trp Ala Pro Leu Ala Thr Ile Thr
                85                  90                  95
Trp Gly Ser Pro Tyr Thr Leu Tyr Phe Leu Leu Ile Met Leu Ile Val
            100                 105                 110
Asn Ile Val Met Ile Val Met Lys Lys Lys Arg Ile Pro
        115                 120                 125
 
           
             14 
             705 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (118)..(705) 
                 
             
           
            14
ggatcgggcg caagcttaac gattcttttt aaaatcatta aattttaaaa caaatttcag     60
acatattgcc aaagttttga tattattact ataatatagt ttgtagagga gaataat       117
atg ggc caa gaa cct atc atc gaa tat caa aat atc aat aaa gtg tat      165
Met Gly Gln Glu Pro Ile Ile Glu Tyr Gln Asn Ile Asn Lys Val Tyr
1               5                   10                  15
ggg gaa aat gtt gcg gtt gaa gat att aac ctt aaa att tac cct ggt      213
Gly Glu Asn Val Ala Val Glu Asp Ile Asn Leu Lys Ile Tyr Pro Gly
            20                  25                  30
gat ttc gtt tgt ttc atc ggt acg agt gga tca ggt aaa aca aca tta      261
Asp Phe Val Cys Phe Ile Gly Thr Ser Gly Ser Gly Lys Thr Thr Leu
        35                  40                  45
atg cgt atg gtt aac cat atg tta aaa cca aca aat ggt act cta tta      309
Met Arg Met Val Asn His Met Leu Lys Pro Thr Asn Gly Thr Leu Leu
    50                  55                  60
ttt aag gga aaa gat atc tct act att aac ccc att gaa tta aga cgc      357
Phe Lys Gly Lys Asp Ile Ser Thr Ile Asn Pro Ile Glu Leu Arg Arg
65                  70                  75                  80
aga att gga tat gtt atc caa aac att ggt tta atg cct cat atg acc      405
Arg Ile Gly Tyr Val Ile Gln Asn Ile Gly Leu Met Pro His Met Thr
                85                  90                  95
att tac gaa aat ata gtt ctt gta cca aaa tta ttg aaa tgg tca gaa      453
Ile Tyr Glu Asn Ile Val Leu Val Pro Lys Leu Leu Lys Trp Ser Glu
            100                 105                 110
gaa gct aaa aga gct aaa gca agg gaa ctt att aaa tta gtt gaa tta      501
Glu Ala Lys Arg Ala Lys Ala Arg Glu Leu Ile Lys Leu Val Glu Leu
        115                 120                 125
ccc gaa gaa tat ttg gat cgc tac cct agt gag ttg tct ggc ggt cag      549
Pro Glu Glu Tyr Leu Asp Arg Tyr Pro Ser Glu Leu Ser Gly Gly Gln
    130                 135                 140
caa caa cgt atc ggt gtc att cgc gct ctt gca gca gac caa gat att      597
Gln Gln Arg Ile Gly Val Ile Arg Ala Leu Ala Ala Asp Gln Asp Ile
145                 150                 155                 160
att tta atg gat gag cct ttt gga gct ctg gat cct att act aga gaa      645
Ile Leu Met Asp Glu Pro Phe Gly Ala Leu Asp Pro Ile Thr Arg Glu
                165                 170                 175
ggt att caa gac ttt agt caa gtc tct tca gga aga aat ggg gga aaa      693
Gly Ile Gln Asp Phe Ser Gln Val Ser Ser Gly Arg Asn Gly Gly Lys
            180                 185                 190
cta tca tct tag                                                      705
Leu Ser Ser
        195
 
           
             15 
             195 
             PRT 
             Streptococcus agalactiae 
           
            15
Met Gly Gln Glu Pro Ile Ile Glu Tyr Gln Asn Ile Asn Lys Val Tyr
1               5                   10                  15
Gly Glu Asn Val Ala Val Glu Asp Ile Asn Leu Lys Ile Tyr Pro Gly
            20                  25                  30
Asp Phe Val Cys Phe Ile Gly Thr Ser Gly Ser Gly Lys Thr Thr Leu
        35                  40                  45
Met Arg Met Val Asn His Met Leu Lys Pro Thr Asn Gly Thr Leu Leu
    50                  55                  60
Phe Lys Gly Lys Asp Ile Ser Thr Ile Asn Pro Ile Glu Leu Arg Arg
65                  70                  75                  80
Arg Ile Gly Tyr Val Ile Gln Asn Ile Gly Leu Met Pro His Met Thr
                85                  90                  95
Ile Tyr Glu Asn Ile Val Leu Val Pro Lys Leu Leu Lys Trp Ser Glu
            100                 105                 110
Glu Ala Lys Arg Ala Lys Ala Arg Glu Leu Ile Lys Leu Val Glu Leu
        115                 120                 125
Pro Glu Glu Tyr Leu Asp Arg Tyr Pro Ser Glu Leu Ser Gly Gly Gln
    130                 135                 140
Gln Gln Arg Ile Gly Val Ile Arg Ala Leu Ala Ala Asp Gln Asp Ile
145                 150                 155                 160
Ile Leu Met Asp Glu Pro Phe Gly Ala Leu Asp Pro Ile Thr Arg Glu
                165                 170                 175
Gly Ile Gln Asp Phe Ser Gln Val Ser Ser Gly Arg Asn Gly Gly Lys
            180                 185                 190
Leu Ser Ser
        195
 
           
             16 
             367 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(366) 
                 
             
           
            16
atc cct tat agt gat gtt ttt gct aca gga gga ttt tta tac tat gta       48
Ile Pro Tyr Ser Asp Val Phe Ala Thr Gly Gly Phe Leu Tyr Tyr Val
1               5                   10                  15
acg att gct cta agt tac ctt tta ggg tct agt atc tgg tta ttt att       96
Thr Ile Ala Leu Ser Tyr Leu Leu Gly Ser Ser Ile Trp Leu Phe Ile
            20                  25                  30
gta cag ttt att gct tac tat gta tct gga att tat ttt tat aaa tta      144
Val Gln Phe Ile Ala Tyr Tyr Val Ser Gly Ile Tyr Phe Tyr Lys Leu
        35                  40                  45
gtt tat tat gtg gca caa agt gaa att gtc tcg ata ggc atg acg ttg      192
Val Tyr Tyr Val Ala Gln Ser Glu Ile Val Ser Ile Gly Met Thr Leu
    50                  55                  60
att ttc tat ata atg aat att gtc tta gga ttc ggt ggt atg tac cca      240
Ile Phe Tyr Ile Met Asn Ile Val Leu Gly Phe Gly Gly Met Tyr Pro
65                  70                  75                  80
ata cag tgg gca tta cct ttt atg ctc att tcg cta tgg ttt tta att      288
Ile Gln Trp Ala Leu Pro Phe Met Leu Ile Ser Leu Trp Phe Leu Ile
                85                  90                  95
aaa ttt tgt gtc gat aat atc gtt gat gaa gca ttt ata ttt tat ggt      336
Lys Phe Cys Val Asp Asn Ile Val Asp Glu Ala Phe Ile Phe Tyr Gly
            100                 105                 110
att tta gca gca ttc tca cta ttt ata gat c                            367
Ile Leu Ala Ala Phe Ser Leu Phe Ile Asp
        115                 120
 
           
             17 
             122 
             PRT 
             Streptococcus agalactiae 
           
            17
Ile Pro Tyr Ser Asp Val Phe Ala Thr Gly Gly Phe Leu Tyr Tyr Val
1               5                   10                  15
Thr Ile Ala Leu Ser Tyr Leu Leu Gly Ser Ser Ile Trp Leu Phe Ile
            20                  25                  30
Val Gln Phe Ile Ala Tyr Tyr Val Ser Gly Ile Tyr Phe Tyr Lys Leu
        35                  40                  45
Val Tyr Tyr Val Ala Gln Ser Glu Ile Val Ser Ile Gly Met Thr Leu
    50                  55                  60
Ile Phe Tyr Ile Met Asn Ile Val Leu Gly Phe Gly Gly Met Tyr Pro
65                  70                  75                  80
Ile Gln Trp Ala Leu Pro Phe Met Leu Ile Ser Leu Trp Phe Leu Ile
                85                  90                  95
Lys Phe Cys Val Asp Asn Ile Val Asp Glu Ala Phe Ile Phe Tyr Gly
            100                 105                 110
Ile Leu Ala Ala Phe Ser Leu Phe Ile Asp
        115                 120
 
           
             18 
             570 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(570) 
                 
             
           
            18
atg agg aaa cgt ttt tcc ttg cta aat ttt att gtt gtt act ttt att       48
Met Arg Lys Arg Phe Ser Leu Leu Asn Phe Ile Val Val Thr Phe Ile
1               5                   10                  15
ttc ttt ttc ttt att ctt ttt ccg ctt tta aac cat aag gga aaa gta       96
Phe Phe Phe Phe Ile Leu Phe Pro Leu Leu Asn His Lys Gly Lys Val
            20                  25                  30
gat gct aat tct agg cag agt gtt acc tac acc aaa gaa gaa ttt ata      144
Asp Ala Asn Ser Arg Gln Ser Val Thr Tyr Thr Lys Glu Glu Phe Ile
        35                  40                  45
caa aaa att gtg cca gat gcg caa gat cta gga aag tcg tac ggt att      192
Gln Lys Ile Val Pro Asp Ala Gln Asp Leu Gly Lys Ser Tyr Gly Ile
    50                  55                  60
cgt cct tca ttt att att gca cag gcg gct ttg gat tct gat ttc gga      240
Arg Pro Ser Phe Ile Ile Ala Gln Ala Ala Leu Asp Ser Asp Phe Gly
65                  70                  75                  80
gag aaa tat agc tat agt atc ata atc tgt tgg ttg ctt gca gaa cca      288
Glu Lys Tyr Ser Tyr Ser Ile Ile Ile Cys Trp Leu Leu Ala Glu Pro
                85                  90                  95
gga acg ccc tca att acc tta aat gat agt agt aca gga aaa aaa cag      336
Gly Thr Pro Ser Ile Thr Leu Asn Asp Ser Ser Thr Gly Lys Lys Gln
            100                 105                 110
gaa aag caa ttt act cat tat aaa tct tgg aag tat tca atg gat gat      384
Glu Lys Gln Phe Thr His Tyr Lys Ser Trp Lys Tyr Ser Met Asp Asp
        115                 120                 125
tac ctt gct cat ata aaa tct gga gcg aca ggc aaa aaa gat tca tat      432
Tyr Leu Ala His Ile Lys Ser Gly Ala Thr Gly Lys Lys Asp Ser Tyr
    130                 135                 140
act ata atg gtg tct gtt aaa aat cca aaa act tta gtg caa aaa tta      480
Thr Ile Met Val Ser Val Lys Asn Pro Lys Thr Leu Val Gln Lys Leu
145                 150                 155                 160
caa gat agt ggt ttt gat aat gac aaa aag tac gct aaa aaa atg acg      528
Gln Asp Ser Gly Phe Asp Asn Asp Lys Lys Tyr Ala Lys Lys Met Thr
                165                 170                 175
gaa atc att gat ttg tat gat tta aca aga tat gat aag tga              570
Glu Ile Ile Asp Leu Tyr Asp Leu Thr Arg Tyr Asp Lys
            180                 185
 
           
             19 
             189 
             PRT 
             Streptococcus agalactiae 
           
            19
Met Arg Lys Arg Phe Ser Leu Leu Asn Phe Ile Val Val Thr Phe Ile
1               5                   10                  15
Phe Phe Phe Phe Ile Leu Phe Pro Leu Leu Asn His Lys Gly Lys Val
            20                  25                  30
Asp Ala Asn Ser Arg Gln Ser Val Thr Tyr Thr Lys Glu Glu Phe Ile
        35                  40                  45
Gln Lys Ile Val Pro Asp Ala Gln Asp Leu Gly Lys Ser Tyr Gly Ile
    50                  55                  60
Arg Pro Ser Phe Ile Ile Ala Gln Ala Ala Leu Asp Ser Asp Phe Gly
65                  70                  75                  80
Glu Lys Tyr Ser Tyr Ser Ile Ile Ile Cys Trp Leu Leu Ala Glu Pro
                85                  90                  95
Gly Thr Pro Ser Ile Thr Leu Asn Asp Ser Ser Thr Gly Lys Lys Gln
            100                 105                 110
Glu Lys Gln Phe Thr His Tyr Lys Ser Trp Lys Tyr Ser Met Asp Asp
        115                 120                 125
Tyr Leu Ala His Ile Lys Ser Gly Ala Thr Gly Lys Lys Asp Ser Tyr
    130                 135                 140
Thr Ile Met Val Ser Val Lys Asn Pro Lys Thr Leu Val Gln Lys Leu
145                 150                 155                 160
Gln Asp Ser Gly Phe Asp Asn Asp Lys Lys Tyr Ala Lys Lys Met Thr
                165                 170                 175
Glu Ile Ile Asp Leu Tyr Asp Leu Thr Arg Tyr Asp Lys
            180                 185
 
           
             20 
             978 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(978) 
                 
             
           
            20
atg ctt gtc atc att ttg atc att gta cta gct agt ctg aca gtg acg       48
Met Leu Val Ile Ile Leu Ile Ile Val Leu Ala Ser Leu Thr Val Thr
1               5                   10                  15
ata att tct tac cca aaa atg acg gaa tta aca aag tcc gtt gaa aaa       96
Ile Ile Ser Tyr Pro Lys Met Thr Glu Leu Thr Lys Ser Val Glu Lys
            20                  25                  30
caa ctt gaa gat aat gct gat aat cta tca gac caa ctg aca tat cag      144
Gln Leu Glu Asp Asn Ala Asp Asn Leu Ser Asp Gln Leu Thr Tyr Gln
        35                  40                  45
ata gaa gtg gcg caa aaa gat caa atc tac gtg act aat cag cta aac      192
Ile Glu Val Ala Gln Lys Asp Gln Ile Tyr Val Thr Asn Gln Leu Asn
    50                  55                  60
cgt atg caa cag gaa att atc agt cgc tta ccg ata tgc gta cag aat      240
Arg Met Gln Gln Glu Ile Ile Ser Arg Leu Pro Ile Cys Val Gln Asn
65                  70                  75                  80
aaa tca gca tta acg gag agt cga gat cga tca gac aaa cgc ttg gaa      288
Lys Ser Ala Leu Thr Glu Ser Arg Asp Arg Ser Asp Lys Arg Leu Glu
                85                  90                  95
ttg att aac tcc aat tta tct cag tca gtt cag aaa atg caa gat tca      336
Leu Ile Asn Ser Asn Leu Ser Gln Ser Val Gln Lys Met Gln Asp Ser
            100                 105                 110
atg aaa aac gct tgg atc aaa tgc gcc aaa ctg ttg agg aaa agc tgg      384
Met Lys Asn Ala Trp Ile Lys Cys Ala Lys Leu Leu Arg Lys Ser Trp
        115                 120                 125
aaa aaa cgc tac aaa cgc gtt gca aac ttc ttt gaa act gta tcg cgt      432
Lys Lys Arg Tyr Lys Arg Val Ala Asn Phe Phe Glu Thr Val Ser Arg
    130                 135                 140
caa cta gag agc gtc aat caa ggt ctg ggt aga tgg aaa ctg tgc caa      480
Gln Leu Glu Ser Val Asn Gln Gly Leu Gly Arg Trp Lys Leu Cys Gln
145                 150                 155                 160
gat gtt ggt acc act gaa caa agt ctg tca aat act aag aca agg gga      528
Asp Val Gly Thr Thr Glu Gln Ser Leu Ser Asn Thr Lys Thr Arg Gly
                165                 170                 175
ata tta ggg gag tta caa ctc ggt caa att ata gaa gat att atg aca      576
Ile Leu Gly Glu Leu Gln Leu Gly Gln Ile Ile Glu Asp Ile Met Thr
            180                 185                 190
gtt agt caa tat gag aga gaa ttt cct acg gtg tct ggc tct tct gag      624
Val Ser Gln Tyr Glu Arg Glu Phe Pro Thr Val Ser Gly Ser Ser Glu
        195                 200                 205
cgt gtt gaa tat gct att aaa tac ctg gaa atg gtc agg gag att ata      672
Arg Val Glu Tyr Ala Ile Lys Tyr Leu Glu Met Val Arg Glu Ile Ile
    210                 215                 220
tct att tgc cta ttg act cta agt ttc tct aga aga tta tta ccg att      720
Ser Ile Cys Leu Leu Thr Leu Ser Phe Ser Arg Arg Leu Leu Pro Ile
225                 230                 235                 240
ggg aga tgc tta tgg aat tgg gtg acc agg ttc aaa tgg aac tct att      768
Gly Arg Cys Leu Trp Asn Trp Val Thr Arg Phe Lys Trp Asn Ser Ile
                245                 250                 255
cgt aat ctt tac tgg gca agt att cgt aaa ttt gca aaa gat ata aac      816
Arg Asn Leu Tyr Trp Ala Ser Ile Arg Lys Phe Ala Lys Asp Ile Asn
            260                 265                 270
aat aag tac tta aat cct cct gaa acg aca aat ttt ggt atc atg ttc      864
Asn Lys Tyr Leu Asn Pro Pro Glu Thr Thr Asn Phe Gly Ile Met Phe
        275                 280                 285
tta cca act gaa ggg ctc tat tct gaa gtg gta aga aat gca aca ttc      912
Leu Pro Thr Glu Gly Leu Tyr Ser Glu Val Val Arg Asn Ala Thr Phe
    290                 295                 300
ttt gat agt cta aga cgt gac gaa aat att gta gta gct gga ccg tca      960
Phe Asp Ser Leu Arg Arg Asp Glu Asn Ile Val Val Ala Gly Pro Ser
305                 310                 315                 320
acc tta tct gct tac taa                                              978
Thr Leu Ser Ala Tyr
                325
 
           
             21 
             325 
             PRT 
             Streptococcus agalactiae 
           
            21
Met Leu Val Ile Ile Leu Ile Ile Val Leu Ala Ser Leu Thr Val Thr
1               5                   10                  15
Ile Ile Ser Tyr Pro Lys Met Thr Glu Leu Thr Lys Ser Val Glu Lys
            20                  25                  30
Gln Leu Glu Asp Asn Ala Asp Asn Leu Ser Asp Gln Leu Thr Tyr Gln
        35                  40                  45
Ile Glu Val Ala Gln Lys Asp Gln Ile Tyr Val Thr Asn Gln Leu Asn
    50                  55                  60
Arg Met Gln Gln Glu Ile Ile Ser Arg Leu Pro Ile Cys Val Gln Asn
65                  70                  75                  80
Lys Ser Ala Leu Thr Glu Ser Arg Asp Arg Ser Asp Lys Arg Leu Glu
                85                  90                  95
Leu Ile Asn Ser Asn Leu Ser Gln Ser Val Gln Lys Met Gln Asp Ser
            100                 105                 110
Met Lys Asn Ala Trp Ile Lys Cys Ala Lys Leu Leu Arg Lys Ser Trp
        115                 120                 125
Lys Lys Arg Tyr Lys Arg Val Ala Asn Phe Phe Glu Thr Val Ser Arg
    130                 135                 140
Gln Leu Glu Ser Val Asn Gln Gly Leu Gly Arg Trp Lys Leu Cys Gln
145                 150                 155                 160
Asp Val Gly Thr Thr Glu Gln Ser Leu Ser Asn Thr Lys Thr Arg Gly
                165                 170                 175
Ile Leu Gly Glu Leu Gln Leu Gly Gln Ile Ile Glu Asp Ile Met Thr
            180                 185                 190
Val Ser Gln Tyr Glu Arg Glu Phe Pro Thr Val Ser Gly Ser Ser Glu
        195                 200                 205
Arg Val Glu Tyr Ala Ile Lys Tyr Leu Glu Met Val Arg Glu Ile Ile
    210                 215                 220
Ser Ile Cys Leu Leu Thr Leu Ser Phe Ser Arg Arg Leu Leu Pro Ile
225                 230                 235                 240
Gly Arg Cys Leu Trp Asn Trp Val Thr Arg Phe Lys Trp Asn Ser Ile
                245                 250                 255
Arg Asn Leu Tyr Trp Ala Ser Ile Arg Lys Phe Ala Lys Asp Ile Asn
            260                 265                 270
Asn Lys Tyr Leu Asn Pro Pro Glu Thr Thr Asn Phe Gly Ile Met Phe
        275                 280                 285
Leu Pro Thr Glu Gly Leu Tyr Ser Glu Val Val Arg Asn Ala Thr Phe
    290                 295                 300
Phe Asp Ser Leu Arg Arg Asp Glu Asn Ile Val Val Ala Gly Pro Ser
305                 310                 315                 320
Thr Leu Ser Ala Tyr
                325
 
           
             22 
             579 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(579) 
                 
             
           
            22
atg cga aaa gaa gtg aca cca gag atg ctt aac tat aat aag tat cct       48
Met Arg Lys Glu Val Thr Pro Glu Met Leu Asn Tyr Asn Lys Tyr Pro
1               5                   10                  15
ggc cca cag ttt att cac ttt gaa aat atc gtt aaa agt gat gat att       96
Gly Pro Gln Phe Ile His Phe Glu Asn Ile Val Lys Ser Asp Asp Ile
            20                  25                  30
gaa ttt caa ctt gtt att aat gaa aaa tca gct ttt gat gtg act gtc      144
Glu Phe Gln Leu Val Ile Asn Glu Lys Ser Ala Phe Asp Val Thr Val
        35                  40                  45
ttt gga caa cgt ttt tct gag att tta tta aaa tat gat ttt atc gtt      192
Phe Gly Gln Arg Phe Ser Glu Ile Leu Leu Lys Tyr Asp Phe Ile Val
    50                  55                  60
ggc gat tgg ggt aac gag cag ttg agg cta aga ggc ttt tac aaa gat      240
Gly Asp Trp Gly Asn Glu Gln Leu Arg Leu Arg Gly Phe Tyr Lys Asp
65                  70                  75                  80
gct agt acg att aga aaa aat agc cgg att tca cgt tta gaa gat tat      288
Ala Ser Thr Ile Arg Lys Asn Ser Arg Ile Ser Arg Leu Glu Asp Tyr
                85                  90                  95
att aaa gag tat tgt aac ttt ggt tgt gct tat ttt gtg ttg gag aat      336
Ile Lys Glu Tyr Cys Asn Phe Gly Cys Ala Tyr Phe Val Leu Glu Asn
            100                 105                 110
cca aat cct aga gat att aaa ttt gat gat gaa aga cct cat aag cgt      384
Pro Asn Pro Arg Asp Ile Lys Phe Asp Asp Glu Arg Pro His Lys Arg
        115                 120                 125
cgt aag tca aga tcc aaa tca caa tca tca aag tca caa act aga aat      432
Arg Lys Ser Arg Ser Lys Ser Gln Ser Ser Lys Ser Gln Thr Arg Asn
    130                 135                 140
aat cgt tcc cag tca aat gcc aat gct cat ttt aca agt aaa aag cgt      480
Asn Arg Ser Gln Ser Asn Ala Asn Ala His Phe Thr Ser Lys Lys Arg
145                 150                 155                 160
aaa gac aca aaa cgc cgt caa gaa cgt cat att aaa gaa gag caa gat      528
Lys Asp Thr Lys Arg Arg Gln Glu Arg His Ile Lys Glu Glu Gln Asp
                165                 170                 175
aag gaa atg acc tct gca aag cag cat ttg tta ttc gta aga aaa aat      576
Lys Glu Met Thr Ser Ala Lys Gln His Leu Leu Phe Val Arg Lys Asn
            180                 185                 190
taa                                                                  579
 
           
             23 
             192 
             PRT 
             Streptococcus agalactiae 
           
            23
Met Arg Lys Glu Val Thr Pro Glu Met Leu Asn Tyr Asn Lys Tyr Pro
1               5                   10                  15
Gly Pro Gln Phe Ile His Phe Glu Asn Ile Val Lys Ser Asp Asp Ile
            20                  25                  30
Glu Phe Gln Leu Val Ile Asn Glu Lys Ser Ala Phe Asp Val Thr Val
        35                  40                  45
Phe Gly Gln Arg Phe Ser Glu Ile Leu Leu Lys Tyr Asp Phe Ile Val
    50                  55                  60
Gly Asp Trp Gly Asn Glu Gln Leu Arg Leu Arg Gly Phe Tyr Lys Asp
65                  70                  75                  80
Ala Ser Thr Ile Arg Lys Asn Ser Arg Ile Ser Arg Leu Glu Asp Tyr
                85                  90                  95
Ile Lys Glu Tyr Cys Asn Phe Gly Cys Ala Tyr Phe Val Leu Glu Asn
            100                 105                 110
Pro Asn Pro Arg Asp Ile Lys Phe Asp Asp Glu Arg Pro His Lys Arg
        115                 120                 125
Arg Lys Ser Arg Ser Lys Ser Gln Ser Ser Lys Ser Gln Thr Arg Asn
    130                 135                 140
Asn Arg Ser Gln Ser Asn Ala Asn Ala His Phe Thr Ser Lys Lys Arg
145                 150                 155                 160
Lys Asp Thr Lys Arg Arg Gln Glu Arg His Ile Lys Glu Glu Gln Asp
                165                 170                 175
Lys Glu Met Thr Ser Ala Lys Gln His Leu Leu Phe Val Arg Lys Asn
            180                 185                 190
 
           
             24 
             609 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(609) 
                 
             
           
            24
atg aca ata aaa aaa gtg tta agt gta aca gga att att tta gtg aca       48
Met Thr Ile Lys Lys Val Leu Ser Val Thr Gly Ile Ile Leu Val Thr
1               5                   10                  15
gta gcg tct cta gct gct tgt agc tca aaa tct cat act act aag acg       96
Val Ala Ser Leu Ala Ala Cys Ser Ser Lys Ser His Thr Thr Lys Thr
            20                  25                  30
ggc aaa aaa gaa gtt aat ttt gca act gtt gga aca acg gca cct ttt      144
Gly Lys Lys Glu Val Asn Phe Ala Thr Val Gly Thr Thr Ala Pro Phe
        35                  40                  45
tct tat gtg aag gat ggg aaa ctg act ggc ttt gat att gaa gta gcc      192
Ser Tyr Val Lys Asp Gly Lys Leu Thr Gly Phe Asp Ile Glu Val Ala
    50                  55                  60
aaa gct gtt ttt aaa ggt tca gat aac tat aaa gtc act ttt aaa aaa      240
Lys Ala Val Phe Lys Gly Ser Asp Asn Tyr Lys Val Thr Phe Lys Lys
65                  70                  75                  80
aca gaa tgg tca tcg gta ttt acc ggc att gat tca gga aag ttt caa      288
Thr Glu Trp Ser Ser Val Phe Thr Gly Ile Asp Ser Gly Lys Phe Gln
                85                  90                  95
atg ggt gga aat aat att tct tat tca tca gag aga tct caa aaa tay      336
Met Gly Gly Asn Asn Ile Ser Tyr Ser Ser Glu Arg Ser Gln Lys Tyr
            100                 105                 110
tta ttt tca tac cca ata ggc tct act cct tca gtt tta gca gtt cct      384
Leu Phe Ser Tyr Pro Ile Gly Ser Thr Pro Ser Val Leu Ala Val Pro
        115                 120                 125
aag aat agt aat atc aaa gct tat aat gat att agt ggt cat aaa aca      432
Lys Asn Ser Asn Ile Lys Ala Tyr Asn Asp Ile Ser Gly His Lys Thr
    130                 135                 140
cag gtt gtc caa gga acg aca act gcc aag caa tta gaa aat ttc aat      480
Gln Val Val Gln Gly Thr Thr Thr Ala Lys Gln Leu Glu Asn Phe Asn
145                 150                 155                 160
aaa gag cat cag aaa aat cct gtt act cta aaa tat act aat gaa aat      528
Lys Glu His Gln Lys Asn Pro Val Thr Leu Lys Tyr Thr Asn Glu Asn
                165                 170                 175
att aca cag att cta acg aat ttg agt gat gga aaa gct gat ttt aaa      576
Ile Thr Gln Ile Leu Thr Asn Leu Ser Asp Gly Lys Ala Asp Phe Lys
            180                 185                 190
ctt ttg acg gac caa ctg tta acg cta tta taa                          609
Leu Leu Thr Asp Gln Leu Leu Thr Leu Leu
        195                 200
 
           
             25 
             202 
             PRT 
             Streptococcus agalactiae 
           
            25
Met Thr Ile Lys Lys Val Leu Ser Val Thr Gly Ile Ile Leu Val Thr
1               5                   10                  15
Val Ala Ser Leu Ala Ala Cys Ser Ser Lys Ser His Thr Thr Lys Thr
            20                  25                  30
Gly Lys Lys Glu Val Asn Phe Ala Thr Val Gly Thr Thr Ala Pro Phe
        35                  40                  45
Ser Tyr Val Lys Asp Gly Lys Leu Thr Gly Phe Asp Ile Glu Val Ala
    50                  55                  60
Lys Ala Val Phe Lys Gly Ser Asp Asn Tyr Lys Val Thr Phe Lys Lys
65                  70                  75                  80
Thr Glu Trp Ser Ser Val Phe Thr Gly Ile Asp Ser Gly Lys Phe Gln
                85                  90                  95
Met Gly Gly Asn Asn Ile Ser Tyr Ser Ser Glu Arg Ser Gln Lys Tyr
            100                 105                 110
Leu Phe Ser Tyr Pro Ile Gly Ser Thr Pro Ser Val Leu Ala Val Pro
        115                 120                 125
Lys Asn Ser Asn Ile Lys Ala Tyr Asn Asp Ile Ser Gly His Lys Thr
    130                 135                 140
Gln Val Val Gln Gly Thr Thr Thr Ala Lys Gln Leu Glu Asn Phe Asn
145                 150                 155                 160
Lys Glu His Gln Lys Asn Pro Val Thr Leu Lys Tyr Thr Asn Glu Asn
                165                 170                 175
Ile Thr Gln Ile Leu Thr Asn Leu Ser Asp Gly Lys Ala Asp Phe Lys
            180                 185                 190
Leu Leu Thr Asp Gln Leu Leu Thr Leu Leu
        195                 200
 
           
             26 
             357 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(357) 
                 
             
           
            26
atg aag aat ata aca aag cta tca act gtt gct tta agc cta cta ctt       48
Met Lys Asn Ile Thr Lys Leu Ser Thr Val Ala Leu Ser Leu Leu Leu
1               5                   10                  15
tgt acg gcg tgt gct gca tca aac acg tct aca tct aaa aca cag tct       96
Cys Thr Ala Cys Ala Ala Ser Asn Thr Ser Thr Ser Lys Thr Gln Ser
            20                  25                  30
cat cat cct aaa caa act aaa ctc aca gat aag caa aaa gaa gaa ccc      144
His His Pro Lys Gln Thr Lys Leu Thr Asp Lys Gln Lys Glu Glu Pro
        35                  40                  45
aaa aac aaa gaa gct gct gat caa gag atg cat ccc caa ggc gct gtt      192
Lys Asn Lys Glu Ala Ala Asp Gln Glu Met His Pro Gln Gly Ala Val
    50                  55                  60
gat ttg aca aaa tat aag gca aaa ccg gtc aaa gat tat gga aaa aaa      240
Asp Leu Thr Lys Tyr Lys Ala Lys Pro Val Lys Asp Tyr Gly Lys Lys
65                  70                  75                  80
atc gat gtt ggt gat ggc aag aaa atg aac att tat gaa act ggt cag      288
Ile Asp Val Gly Asp Gly Lys Lys Met Asn Ile Tyr Glu Thr Gly Gln
                85                  90                  95
gga aaa att cca att gtt ttt att cct ggt caa gct gag att cgc cac      336
Gly Lys Ile Pro Ile Val Phe Ile Pro Gly Gln Ala Glu Ile Arg His
            100                 105                 110
gct atg ctt ata aga att taa                                          357
Ala Met Leu Ile Arg Ile
        115
 
           
             27 
             118 
             PRT 
             Streptococcus agalactiae 
           
            27
Met Lys Asn Ile Thr Lys Leu Ser Thr Val Ala Leu Ser Leu Leu Leu
1               5                   10                  15
Cys Thr Ala Cys Ala Ala Ser Asn Thr Ser Thr Ser Lys Thr Gln Ser
            20                  25                  30
His His Pro Lys Gln Thr Lys Leu Thr Asp Lys Gln Lys Glu Glu Pro
        35                  40                  45
Lys Asn Lys Glu Ala Ala Asp Gln Glu Met His Pro Gln Gly Ala Val
    50                  55                  60
Asp Leu Thr Lys Tyr Lys Ala Lys Pro Val Lys Asp Tyr Gly Lys Lys
65                  70                  75                  80
Ile Asp Val Gly Asp Gly Lys Lys Met Asn Ile Tyr Glu Thr Gly Gln
                85                  90                  95
Gly Lys Ile Pro Ile Val Phe Ile Pro Gly Gln Ala Glu Ile Arg His
            100                 105                 110
Ala Met Leu Ile Arg Ile
        115
 
           
             28 
             1191 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(1191) 
                 
             
           
            28
gtg aat gaa tcg acc atc aga aaa gaa ttt aaa ata gtt gtt ttt aaa       48
Val Asn Glu Ser Thr Ile Arg Lys Glu Phe Lys Ile Val Val Phe Lys
1               5                   10                  15
tgg atc tta aat aat caa gca gtt att gct ctc atg att acc ttt ttg       96
Trp Ile Leu Asn Asn Gln Ala Val Ile Ala Leu Met Ile Thr Phe Leu
            20                  25                  30
gta ttt tta acg att ttt att ttt acc aaa atc tct ttt atg ttt aaa      144
Val Phe Leu Thr Ile Phe Ile Phe Thr Lys Ile Ser Phe Met Phe Lys
        35                  40                  45
cct gtg ttt gat ttt ctt gct gtg ctg ata ttg ccg ctt gta att tct      192
Pro Val Phe Asp Phe Leu Ala Val Leu Ile Leu Pro Leu Val Ile Ser
    50                  55                  60
ggc ttg ctt tat tac cta tta aaa cct atg gtt aca ttt tta gag aag      240
Gly Leu Leu Tyr Tyr Leu Leu Lys Pro Met Val Thr Phe Leu Glu Lys
65                  70                  75                  80
cgg gga att aag cgt gta aca gcg ata tta tca gtt ttt act att ata      288
Arg Gly Ile Lys Arg Val Thr Ala Ile Leu Ser Val Phe Thr Ile Ile
                85                  90                  95
atc ctt ctg tta att tgg gca atg tct agt ttt att ccc atg atg agt      336
Ile Leu Leu Leu Ile Trp Ala Met Ser Ser Phe Ile Pro Met Met Ser
            100                 105                 110
aat caa tta cgc cat ttt atg gaa gat ctc cct tca tat gtg aat aaa      384
Asn Gln Leu Arg His Phe Met Glu Asp Leu Pro Ser Tyr Val Asn Lys
        115                 120                 125
gtg caa atg gaa aca agt tcg ttt ata gat cac aac cct tgg tta aaa      432
Val Gln Met Glu Thr Ser Ser Phe Ile Asp His Asn Pro Trp Leu Lys
    130                 135                 140
tct tat aaa ggg gaa ata tcg agc atg tta tct aat atc agt agc caa      480
Ser Tyr Lys Gly Glu Ile Ser Ser Met Leu Ser Asn Ile Ser Ser Gln
145                 150                 155                 160
gcg gtc tct tat gct gaa aaa ttt tca aag aat gtt tta gat tgg gca      528
Ala Val Ser Tyr Ala Glu Lys Phe Ser Lys Asn Val Leu Asp Trp Ala
                165                 170                 175
gga aat tta gct agt aca gtt gca cgt gtg aca gta gca aca atc atg      576
Gly Asn Leu Ala Ser Thr Val Ala Arg Val Thr Val Ala Thr Ile Met
            180                 185                 190
gct ccc ttt att ttg ttt tat ctt tta aga gat agt cgc aac atg aag      624
Ala Pro Phe Ile Leu Phe Tyr Leu Leu Arg Asp Ser Arg Asn Met Lys
        195                 200                 205
aat ggt ttc tta atg gtt tta cca acc aaa cta cgc caa cca gct gat      672
Asn Gly Phe Leu Met Val Leu Pro Thr Lys Leu Arg Gln Pro Ala Asp
    210                 215                 220
cgt att ttg cga gaa atg aat agt caa atg tca gga tat gtg caa gga      720
Arg Ile Leu Arg Glu Met Asn Ser Gln Met Ser Gly Tyr Val Gln Gly
225                 230                 235                 240
caa atc att gtt gct att act gtt ggt gtt att ttt tca ata atg tat      768
Gln Ile Ile Val Ala Ile Thr Val Gly Val Ile Phe Ser Ile Met Tyr
                245                 250                 255
agt att ata ggc ctt aga tat ggc gtg aca tta ggg att att gcc ggt      816
Ser Ile Ile Gly Leu Arg Tyr Gly Val Thr Leu Gly Ile Ile Ala Gly
            260                 265                 270
gtg tta aat atg gtt ccc tat ttg gga agt ttt gtc gcc caa att cca      864
Val Leu Asn Met Val Pro Tyr Leu Gly Ser Phe Val Ala Gln Ile Pro
        275                 280                 285
gtg ttt atc tta gcg ctt gtc gca gga cct gtt atg gtt gtt aaa gtt      912
Val Phe Ile Leu Ala Leu Val Ala Gly Pro Val Met Val Val Lys Val
    290                 295                 300
gcg att gtt ttt gtt att gag caa act cta gag gga cgc ttt gtc tca      960
Ala Ile Val Phe Val Ile Glu Gln Thr Leu Glu Gly Arg Phe Val Ser
305                 310                 315                 320
cct ttg gtt tta ggt aat aaa ctt agc att cat cca att aca att atg     1008
Pro Leu Val Leu Gly Asn Lys Leu Ser Ile His Pro Ile Thr Ile Met
                325                 330                 335
ttt att tta tta acc tct gga gcg atg ttt ggt gtt tgg gga gta ttc     1056
Phe Ile Leu Leu Thr Ser Gly Ala Met Phe Gly Val Trp Gly Val Phe
            340                 345                 350
ctc agt att ccg att tat gca tct atc aaa gtt gtt gtt aaa gaa ttg     1104
Leu Ser Ile Pro Ile Tyr Ala Ser Ile Lys Val Val Val Lys Glu Leu
        355                 360                 365
ttt gat tgg tac aaa gct gtc agt ggg cta tat aca ata gat gtt gtt     1152
Phe Asp Trp Tyr Lys Ala Val Ser Gly Leu Tyr Thr Ile Asp Val Val
    370                 375                 380
act gaa gaa aga agt gaa gaa gtt aaa aat gtt gaa tag                 1191
Thr Glu Glu Arg Ser Glu Glu Val Lys Asn Val Glu
385                 390                 395
 
           
             29 
             396 
             PRT 
             Streptococcus agalactiae 
           
            29
Val Asn Glu Ser Thr Ile Arg Lys Glu Phe Lys Ile Val Val Phe Lys
1               5                   10                  15
Trp Ile Leu Asn Asn Gln Ala Val Ile Ala Leu Met Ile Thr Phe Leu
            20                  25                  30
Val Phe Leu Thr Ile Phe Ile Phe Thr Lys Ile Ser Phe Met Phe Lys
        35                  40                  45
Pro Val Phe Asp Phe Leu Ala Val Leu Ile Leu Pro Leu Val Ile Ser
    50                  55                  60
Gly Leu Leu Tyr Tyr Leu Leu Lys Pro Met Val Thr Phe Leu Glu Lys
65                  70                  75                  80
Arg Gly Ile Lys Arg Val Thr Ala Ile Leu Ser Val Phe Thr Ile Ile
                85                  90                  95
Ile Leu Leu Leu Ile Trp Ala Met Ser Ser Phe Ile Pro Met Met Ser
            100                 105                 110
Asn Gln Leu Arg His Phe Met Glu Asp Leu Pro Ser Tyr Val Asn Lys
        115                 120                 125
Val Gln Met Glu Thr Ser Ser Phe Ile Asp His Asn Pro Trp Leu Lys
    130                 135                 140
Ser Tyr Lys Gly Glu Ile Ser Ser Met Leu Ser Asn Ile Ser Ser Gln
145                 150                 155                 160
Ala Val Ser Tyr Ala Glu Lys Phe Ser Lys Asn Val Leu Asp Trp Ala
                165                 170                 175
Gly Asn Leu Ala Ser Thr Val Ala Arg Val Thr Val Ala Thr Ile Met
            180                 185                 190
Ala Pro Phe Ile Leu Phe Tyr Leu Leu Arg Asp Ser Arg Asn Met Lys
        195                 200                 205
Asn Gly Phe Leu Met Val Leu Pro Thr Lys Leu Arg Gln Pro Ala Asp
    210                 215                 220
Arg Ile Leu Arg Glu Met Asn Ser Gln Met Ser Gly Tyr Val Gln Gly
225                 230                 235                 240
Gln Ile Ile Val Ala Ile Thr Val Gly Val Ile Phe Ser Ile Met Tyr
                245                 250                 255
Ser Ile Ile Gly Leu Arg Tyr Gly Val Thr Leu Gly Ile Ile Ala Gly
            260                 265                 270
Val Leu Asn Met Val Pro Tyr Leu Gly Ser Phe Val Ala Gln Ile Pro
        275                 280                 285
Val Phe Ile Leu Ala Leu Val Ala Gly Pro Val Met Val Val Lys Val
    290                 295                 300
Ala Ile Val Phe Val Ile Glu Gln Thr Leu Glu Gly Arg Phe Val Ser
305                 310                 315                 320
Pro Leu Val Leu Gly Asn Lys Leu Ser Ile His Pro Ile Thr Ile Met
                325                 330                 335
Phe Ile Leu Leu Thr Ser Gly Ala Met Phe Gly Val Trp Gly Val Phe
            340                 345                 350
Leu Ser Ile Pro Ile Tyr Ala Ser Ile Lys Val Val Val Lys Glu Leu
        355                 360                 365
Phe Asp Trp Tyr Lys Ala Val Ser Gly Leu Tyr Thr Ile Asp Val Val
    370                 375                 380
Thr Glu Glu Arg Ser Glu Glu Val Lys Asn Val Glu
385                 390                 395
 
           
             30 
             1230 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(1230) 
                 
             
           
            30
atg ttt atg gga atc cca caa tat ttc ttc tac ctt atc tta gct gtc       48
Met Phe Met Gly Ile Pro Gln Tyr Phe Phe Tyr Leu Ile Leu Ala Val
1               5                   10                  15
cta cca att tac atc ggc tta ttc ttt aag aag cgt ttt gcc tta tat       96
Leu Pro Ile Tyr Ile Gly Leu Phe Phe Lys Lys Arg Phe Ala Leu Tyr
            20                  25                  30
gag att att ttt agt cta agt ttt att gta atg atg ttg act ggt agt      144
Glu Ile Ile Phe Ser Leu Ser Phe Ile Val Met Met Leu Thr Gly Ser
        35                  40                  45
act ttt aat caa ttg aag tca cta ttg gca tac gtt gtc gga cag tct      192
Thr Phe Asn Gln Leu Lys Ser Leu Leu Ala Tyr Val Val Gly Gln Ser
    50                  55                  60
ctg cta gtt ttt atc tat aaa gct tac cgg aaa cga ttt aat cat act      240
Leu Leu Val Phe Ile Tyr Lys Ala Tyr Arg Lys Arg Phe Asn His Thr
65                  70                  75                  80
ttg gtc ttt tat gta acg gtt tgt tta tct att ttt ccg cta ttt ttg      288
Leu Val Phe Tyr Val Thr Val Cys Leu Ser Ile Phe Pro Leu Phe Leu
                85                  90                  95
gta aaa tta att cca gct ata tct gag gat ggg cat cag tca ctt ttt      336
Val Lys Leu Ile Pro Ala Ile Ser Glu Asp Gly His Gln Ser Leu Phe
            100                 105                 110
ggg ttt cta gga att tct tac ctt act ttt aga gct gtt gct atg att      384
Gly Phe Leu Gly Ile Ser Tyr Leu Thr Phe Arg Ala Val Ala Met Ile
        115                 120                 125
att gaa atg aga gac ggt gtc ttg aaa gaa ttt act tta tgg gaa ttc      432
Ile Glu Met Arg Asp Gly Val Leu Lys Glu Phe Thr Leu Trp Glu Phe
    130                 135                 140
tta aga ttt tta ctc ttc ttt cca act ttc tca agt gga cca att gat      480
Leu Arg Phe Leu Leu Phe Phe Pro Thr Phe Ser Ser Gly Pro Ile Asp
145                 150                 155                 160
cgt ttt aaa cga ttc aat gag gat tac att aat atc cca gat cga aac      528
Arg Phe Lys Arg Phe Asn Glu Asp Tyr Ile Asn Ile Pro Asp Arg Asn
                165                 170                 175
gaa ctc cta gat atg tta ggt caa gcg att cat tat ttg atg tta ggt      576
Glu Leu Leu Asp Met Leu Gly Gln Ala Ile His Tyr Leu Met Leu Gly
            180                 185                 190
ttt ctc tat aag ttt att tta gcc tat att ttt gga agt ctg att atg      624
Phe Leu Tyr Lys Phe Ile Leu Ala Tyr Ile Phe Gly Ser Leu Ile Met
        195                 200                 205
cct cct cta aaa gaa tta gcg cta gaa cag ggt ggt gtg ttt aat tgg      672
Pro Pro Leu Lys Glu Leu Ala Leu Glu Gln Gly Gly Val Phe Asn Trp
    210                 215                 220
cca aca ctt ggg gtt atg tat gcc ttt ggt ttt gat ttg ttc ttt gat      720
Pro Thr Leu Gly Val Met Tyr Ala Phe Gly Phe Asp Leu Phe Phe Asp
225                 230                 235                 240
ttt gca ggt tac aca atg ttt gcg ttg gct att tct aac cta atg ggg      768
Phe Ala Gly Tyr Thr Met Phe Ala Leu Ala Ile Ser Asn Leu Met Gly
                245                 250                 255
att aag tct ccg att aac ttt gac aaa cct ttc aaa tca cgc gac cta      816
Ile Lys Ser Pro Ile Asn Phe Asp Lys Pro Phe Lys Ser Arg Asp Leu
            260                 265                 270
aaa gaa ttt tgg aat aga tgg cat atg agc ctt tct ttc tgg ttt aga      864
Lys Glu Phe Trp Asn Arg Trp His Met Ser Leu Ser Phe Trp Phe Arg
        275                 280                 285
gac ttt gtt ttc atg agg ctt gtt aag ctt tta gtt aaa aat aaa gtt      912
Asp Phe Val Phe Met Arg Leu Val Lys Leu Leu Val Lys Asn Lys Val
    290                 295                 300
ttt aaa aac cgt aat gtt act tca agt gta gct tat att atc aat atg      960
Phe Lys Asn Arg Asn Val Thr Ser Ser Val Ala Tyr Ile Ile Asn Met
305                 310                 315                 320
ctt ctt atg gga ttc tgg cat ggg tta act tgg tac tat ata gcc tat     1008
Leu Leu Met Gly Phe Trp His Gly Leu Thr Trp Tyr Tyr Ile Ala Tyr
                325                 330                 335
ggt ctc ttt cat ggg att ggc cta gtt att aat gac gct tgg gta cgt     1056
Gly Leu Phe His Gly Ile Gly Leu Val Ile Asn Asp Ala Trp Val Arg
            340                 345                 350
aag aag aaa aat ayt aat aaa gaa aga aga ttg gct aaa aaa cca ctt     1104
Lys Lys Lys Asn Xaa Asn Lys Glu Arg Arg Leu Ala Lys Lys Pro Leu
        355                 360                 365
tta cca gaa aac aaa tgg act tat gct ttg ggt gtc ttc atc acc ttt     1152
Leu Pro Glu Asn Lys Trp Thr Tyr Ala Leu Gly Val Phe Ile Thr Phe
    370                 375                 380
aat gta gtt atg ttt tct ttc ttg att ttt tca gga ttt tta gat ctt     1200
Asn Val Val Met Phe Ser Phe Leu Ile Phe Ser Gly Phe Leu Asp Leu
385                 390                 395                 400
ttg tgg ttc cca caa ccg cat aac aaa taa                             1230
Leu Trp Phe Pro Gln Pro His Asn Lys
                405
 
           
             31 
             409 
             PRT 
             Streptococcus agalactiae 
             
               misc_feature 
               (357)..(357) 
               The ′Xaa′ at location 357 stands for Thr, or
      Ile. 
             
           
            31
Met Phe Met Gly Ile Pro Gln Tyr Phe Phe Tyr Leu Ile Leu Ala Val
1               5                   10                  15
Leu Pro Ile Tyr Ile Gly Leu Phe Phe Lys Lys Arg Phe Ala Leu Tyr
            20                  25                  30
Glu Ile Ile Phe Ser Leu Ser Phe Ile Val Met Met Leu Thr Gly Ser
        35                  40                  45
Thr Phe Asn Gln Leu Lys Ser Leu Leu Ala Tyr Val Val Gly Gln Ser
    50                  55                  60
Leu Leu Val Phe Ile Tyr Lys Ala Tyr Arg Lys Arg Phe Asn His Thr
65                  70                  75                  80
Leu Val Phe Tyr Val Thr Val Cys Leu Ser Ile Phe Pro Leu Phe Leu
                85                  90                  95
Val Lys Leu Ile Pro Ala Ile Ser Glu Asp Gly His Gln Ser Leu Phe
            100                 105                 110
Gly Phe Leu Gly Ile Ser Tyr Leu Thr Phe Arg Ala Val Ala Met Ile
        115                 120                 125
Ile Glu Met Arg Asp Gly Val Leu Lys Glu Phe Thr Leu Trp Glu Phe
    130                 135                 140
Leu Arg Phe Leu Leu Phe Phe Pro Thr Phe Ser Ser Gly Pro Ile Asp
145                 150                 155                 160
Arg Phe Lys Arg Phe Asn Glu Asp Tyr Ile Asn Ile Pro Asp Arg Asn
                165                 170                 175
Glu Leu Leu Asp Met Leu Gly Gln Ala Ile His Tyr Leu Met Leu Gly
            180                 185                 190
Phe Leu Tyr Lys Phe Ile Leu Ala Tyr Ile Phe Gly Ser Leu Ile Met
        195                 200                 205
Pro Pro Leu Lys Glu Leu Ala Leu Glu Gln Gly Gly Val Phe Asn Trp
    210                 215                 220
Pro Thr Leu Gly Val Met Tyr Ala Phe Gly Phe Asp Leu Phe Phe Asp
225                 230                 235                 240
Phe Ala Gly Tyr Thr Met Phe Ala Leu Ala Ile Ser Asn Leu Met Gly
                245                 250                 255
Ile Lys Ser Pro Ile Asn Phe Asp Lys Pro Phe Lys Ser Arg Asp Leu
            260                 265                 270
Lys Glu Phe Trp Asn Arg Trp His Met Ser Leu Ser Phe Trp Phe Arg
        275                 280                 285
Asp Phe Val Phe Met Arg Leu Val Lys Leu Leu Val Lys Asn Lys Val
    290                 295                 300
Phe Lys Asn Arg Asn Val Thr Ser Ser Val Ala Tyr Ile Ile Asn Met
305                 310                 315                 320
Leu Leu Met Gly Phe Trp His Gly Leu Thr Trp Tyr Tyr Ile Ala Tyr
                325                 330                 335
Gly Leu Phe His Gly Ile Gly Leu Val Ile Asn Asp Ala Trp Val Arg
            340                 345                 350
Lys Lys Lys Asn Xaa Asn Lys Glu Arg Arg Leu Ala Lys Lys Pro Leu
        355                 360                 365
Leu Pro Glu Asn Lys Trp Thr Tyr Ala Leu Gly Val Phe Ile Thr Phe
    370                 375                 380
Asn Val Val Met Phe Ser Phe Leu Ile Phe Ser Gly Phe Leu Asp Leu
385                 390                 395                 400
Leu Trp Phe Pro Gln Pro His Asn Lys
                405
 
           
             32 
             100 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(99) 
                 
             
           
            32
atg aat aaa ata acg aca tta tca acc atc gcc ctg act tta atg ctt       48
Met Asn Lys Ile Thr Thr Leu Ser Thr Ile Ala Leu Thr Leu Met Leu
1               5                   10                  15
tgc gtt gga tgt tct gcc aat aaa gat aat caa aaa act aaa act gag       96
Cys Val Gly Cys Ser Ala Asn Lys Asp Asn Gln Lys Thr Lys Thr Glu
            20                  25                  30
gat c                                                                100
Asp
 
           
             33 
             33 
             PRT 
             Streptococcus agalactiae 
           
            33
Met Asn Lys Ile Thr Thr Leu Ser Thr Ile Ala Leu Thr Leu Met Leu
1               5                   10                  15
Cys Val Gly Cys Ser Ala Asn Lys Asp Asn Gln Lys Thr Lys Thr Glu
            20                  25                  30
Asp
 
           
             34 
             654 
             DNA 
             Streptococcus agalactiae 
             
               CDS 
               (1)..(654) 
                 
             
           
            34
gat cga ggc tat caa gaa gca atg gct aaa cta agg aaa act tac ggc       48
Asp Arg Gly Tyr Gln Glu Ala Met Ala Lys Leu Arg Lys Thr Tyr Gly
1               5                   10                  15
gaa tat ggt tta ggg gtt tct aca gga tta gat tta cct gaa tca gaa       96
Glu Tyr Gly Leu Gly Val Ser Thr Gly Leu Asp Leu Pro Glu Ser Glu
            20                  25                  30
ggt tat gta cct gga aaa tac agc tta gga aca act cta atg gaa tcg      144
Gly Tyr Val Pro Gly Lys Tyr Ser Leu Gly Thr Thr Leu Met Glu Ser
        35                  40                  45
ttc ggt cag tat gat gcc tat aca cca atg caa ctt ggt cag tat atc      192
Phe Gly Gln Tyr Asp Ala Tyr Thr Pro Met Gln Leu Gly Gln Tyr Ile
    50                  55                  60
tca act att gcg aat aat ggg aat cgt tta gca cct cac gtg gtt tca      240
Ser Thr Ile Ala Asn Asn Gly Asn Arg Leu Ala Pro His Val Val Ser
65                  70                  75                  80
gat atc tat gaa ggg aat gat tct aat aag ttc gct caa ttg gtt cgt      288
Asp Ile Tyr Glu Gly Asn Asp Ser Asn Lys Phe Ala Gln Leu Val Arg
                85                  90                  95
tca atc act cct aaa aca cta aat aag ata gct atc tca gat caa gag      336
Ser Ile Thr Pro Lys Thr Leu Asn Lys Ile Ala Ile Ser Asp Gln Glu
            100                 105                 110
tta gcc att att caa gaa ggt ttt tat aac gtt gtc aat agt gga agt      384
Leu Ala Ile Ile Gln Glu Gly Phe Tyr Asn Val Val Asn Ser Gly Ser
        115                 120                 125
ggc tat gca act ggt acg tca atg agg ggg aat gtg aca acc att agy      432
Gly Tyr Ala Thr Gly Thr Ser Met Arg Gly Asn Val Thr Thr Ile Ser
    130                 135                 140
ggt aaa act ggt acc gct gaa aca ttt gct aaa aat ata aat gga caa      480
Gly Lys Thr Gly Thr Ala Glu Thr Phe Ala Lys Asn Ile Asn Gly Gln
145                 150                 155                 160
aca gtt tct acc tac aac tta aac gct att gcc tac gat act aat cgt      528
Thr Val Ser Thr Tyr Asn Leu Asn Ala Ile Ala Tyr Asp Thr Asn Arg
                165                 170                 175
aaa ata gca gta gcg gta atg tat ccg cat gtt aca act gat aca aca      576
Lys Ile Ala Val Ala Val Met Tyr Pro His Val Thr Thr Asp Thr Thr
            180                 185                 190
aaa tcc cat caa tta gtt gca cga gat atg att gat caa tat att tca      624
Lys Ser His Gln Leu Val Ala Arg Asp Met Ile Asp Gln Tyr Ile Ser
        195                 200                 205
cag tca cag gac aat aag aga gga cat tga                              654
Gln Ser Gln Asp Asn Lys Arg Gly His
    210                 215
 
           
             35 
             217 
             PRT 
             Streptococcus agalactiae 
           
            35
Asp Arg Gly Tyr Gln Glu Ala Met Ala Lys Leu Arg Lys Thr Tyr Gly
1               5                   10                  15
Glu Tyr Gly Leu Gly Val Ser Thr Gly Leu Asp Leu Pro Glu Ser Glu
            20                  25                  30
Gly Tyr Val Pro Gly Lys Tyr Ser Leu Gly Thr Thr Leu Met Glu Ser
        35                  40                  45
Phe Gly Gln Tyr Asp Ala Tyr Thr Pro Met Gln Leu Gly Gln Tyr Ile
    50                  55                  60
Ser Thr Ile Ala Asn Asn Gly Asn Arg Leu Ala Pro His Val Val Ser
65                  70                  75                  80
Asp Ile Tyr Glu Gly Asn Asp Ser Asn Lys Phe Ala Gln Leu Val Arg
                85                  90                  95
Ser Ile Thr Pro Lys Thr Leu Asn Lys Ile Ala Ile Ser Asp Gln Glu
            100                 105                 110
Leu Ala Ile Ile Gln Glu Gly Phe Tyr Asn Val Val Asn Ser Gly Ser
        115                 120                 125
Gly Tyr Ala Thr Gly Thr Ser Met Arg Gly Asn Val Thr Thr Ile Ser
    130                 135                 140
Gly Lys Thr Gly Thr Ala Glu Thr Phe Ala Lys Asn Ile Asn Gly Gln
145                 150                 155                 160
Thr Val Ser Thr Tyr Asn Leu Asn Ala Ile Ala Tyr Asp Thr Asn Arg
                165                 170                 175
Lys Ile Ala Val Ala Val Met Tyr Pro His Val Thr Thr Asp Thr Thr
            180                 185                 190
Lys Ser His Gln Leu Val Ala Arg Asp Met Ile Asp Gln Tyr Ile Ser
        195                 200                 205
Gln Ser Gln Asp Asn Lys Arg Gly His
    210                 215