Abstract:
The present invention is a compound represented by the following formula (1) or (2):                          
 
where one of R 1  and R 2  is hydrogen and the other one is hydrogen or glucose residue. The compound is effective for reforming obese constitution, suppressing or preventing obesity. It is also effective for skin whitening.

Description:
FIELD OF THE INVENTION 
     The present invention relates to dihydroxyphenyl compounds and glucoside compounds thereof which are useful to reform obese constitution by promoting shrinkage of general or topical fat tissues, or to suppress or prevent obesity by preventing the fat tissue from swelling. 
     The present invention relates also to dihydroxyphenyl compounds and glucoside compounds thereof which are effective for skin whitening. 
     DESCRIPTION OF THE PRIOR ART 
     Intercorporal fat is neutral fat present in white adipose tissues produced from surplus energy intake remaining after energy consumption. Obesity due to heavily accumulated intercorporal fat is not only aesthetically unfavorable, but also causes various diseases, such as arteriosclerosis. Recently, more and more people suffer from obesity due to excessive eating, lack of exercise, and/or stress. On the other hand, a slim and tight body is yearned for particularly by women from the viewpoint of appearance. Accumulation of subcutaneous fat is unfavorable for health, so that reduction of the fat or prevention of the accumulation thereof is important. 
     Meanwhile, capsaicin contained in Capsicum is known to be capable of preventing obesity by bonding to blood albumin and secreting hormone which promotes adrenal metabolism and activates energy metabolism in lever or fat cells (Kazuo Iwai and Nobuji Nakatani, Function of Spice Ingredients in Foods, 97(1989), Koseikan, Tokyo). However, capsaicin is strongly irritant and thus has limited applications or is used in a limited amount. 
     The present inventors speculated that it is difficult to diminish accumulated fat cells, but it is easier to make the cells smaller by decomposing lipid droplets in the cells. 
     The lipid droplet can be decomposed with an enzyme, phospholipase C, in a similar manner as protein. However, it is surrounded by a hydrophobic phospholipid membrane, so that the enzyme present in endoplasmic reticulum which is a mass of water cannot obtain access to the lipid droplet. 
     Meanwhile, sympathetic nerves are activated by taking exercise to secrete fat decomposing hormone. This hormone is believed to remove the phospholipid membrane to allow the enzyme to obtain access the lipid droplets to thereby promote lypolysis. Therefore, the present inventors endeavored to find a substance which promotes accessibility of the enzyme to the lipid droplets in a similar manner as the hormone. 
     As a result, the present inventors found that raspberry ketone, zingerone and derivatives thereof promote decomposition of fat accumulated in fat tissues and, thus, are effective to suppress obesity or to reform obese constitution (Japanese Patent Application Laid-open No. 2000-169325). However, both raspberry ketone and zingerone have their peculiar odor and taste and consequently their amounts of dosage and versatility are limited. 
     Thus, a lypolysis promoter, skin cosmetic composition, and food or drink composition which have satisfactory versatility and an effect of preventing formation of or reducing subcutaneous fat are desired. 
     Meanwhile, there is strong desire for white skin and, accordingly, it is desired to prevent or lighten erythema, melanization, stains, or freckles due to skin damages caused by UV light. To lighten the damage caused by UV light leads to suppressing photo-aging which causes wrinkles. Thus, a skin cosmetic composition having effects of preventing erythema and of whitening is desired. 
     In view of the above discussion, it is an object of the present invention to provide a substance which is effective for reforming obese constitution by promoting shrinkage of general or topical fat tissues, or suppressing or preventing obesity by preventing swelling of the fat tissues, and which substance has no odor or taste and has excellent versatility. 
     Another object of the present invention is to provide a skin cosmetic composition which has an excellent whitening effect. 
     SUMMARY OF THE INVENTION 
     The present inventors have found that a compound represented by the following formula (1) or (2): 
                                
where one of R 1  and R 2  is hydrogen and the other one is hydrogen or glucose residue, is effective for reforming obese constitution or suppressing or preventing obesity by promoting lypolysis and has whitening effect to thereby attain the aforesaid objects.
 
     Thus, the present invention is a compound represented by the formula (1) or (2). 
     Further, the present invention is a lipolysis promoter or a melanogenesis inhibitor represented by the formula (1) or (2). Still further, the present invention is a skin cosmetic composition or a food or drink composition comprising the compound represented by the formula (1) or (2). 
     Also provided is a method for reducing a body weight by orally dosing or applying to skin the lyolysis promoter represented by the formula (1) or (2). 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  shows a GC-MS total ion chromatogram (top) and a mass spectrum (bottom) of 4-(3′,4′-dihydroxyphenyl)-butane-2-one of the present invention. 
         FIG. 2  shows a  13 C-NMR spectrum of 4-(3′,4′-dihydroxyphenyl)-butane-2-one of the present invention. 
         FIG. 3  shows a GC-MS total ion chromatogram and a mass spectrum of 4-(3′,4′-dihydroxyphenyl)-butane-2-ol of the present invention. 
         FIG. 4  shows a  13 C-NMR spectrum of 4-(3′,4′-dihydroxyphenyl)-butane-2-ol of the present invention. 
         FIG. 5  shows a  1 H-NMR spectrum of 4-(3′,4′-dihydroxyphenyl)-butane-2-ol of the present invention. 
     
    
    
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     The present invention will be explained in detail. 
     Japanese Patent Application No.11-42937 by the present applicant was published on Sep. 5, 2000(JP Laid-open No. 2000-239143), which describes some species of the present compound as an active ingredient for a skin cosmetic composition having melanogenesis inhibition effect. 
     The compound of the aforesaid formula (1) wherein R 1  and R 2  are both hydrogen, 4-(3′,4′-dihydroxyphenyl)-butane-2-one, may be easily prepared by the method described in the above application. Specifically, it may be easily prepared by cleavage of C—O bond of methylether of zingerone, [4-(3′-methoxy-4′-hydroxyphenyl)-butane-2-one], which is contained in ginger, in a known manner with, for example, boron tribromide or hydriodic acid. 
     The compound of the formula (1) wherein R 1  or R 2 is glucose residue may be obtained by extracting it from plants, such as raspberries, with an appropriate solvent and, if necessary, condensing or evaporating the solvent to dryness in a known method(see Phytochemistry, Vol. 29, No.12, 3853–3858(1990)). Alternatively, it may be prepared by condensing 3,4-dihydroxy benzaldehyde with acetone and reducing the condensate by a conventional method. 
     The compound of the aforesaid formula (2) wherein R 1  and R 2  are both hydrogen, 4-(3′,4′-dihydroxyphenyl)-butane-2-ol, may be prepared in a similar manner as described above, starting from 4-(3′-methoxy-4′-hydroxyphenyl)-butane-2-ol obtained by reducing zingerone with sodium borohydride. It may also be prepared by reducing the compound of the formula (1) by a conventional method. 
     The compound of the formula (1) or (2) may be used alone or in combination of two or more of them as a lypolysis promoter or a whitening agent. An amount of the present compound to be incorporated in a skin cosmetic composition or a food or drink composition intended to be used for reducing weight may vary depending on the form of the composition. In the skin cosmetic composition for reducing weight, the present compound is incorporated preferably in an amount of from 0.001 to 20 wt %, more preferably from 0.01 to 5.0 wt %, most preferably from 0.05 to 3.0 wt % based on the weight of the composition. In the food or drink composition for reducing weight, the present compound is incorporated preferably in an amount of from 0.001 to 20 wt %, more preferably from 0.01 to 10.0 wt %, most preferably from 0.01 to 3.0 wt % based on the weight of the composition. If the amount is less than the aforesaid lower limit, an effect of the present invention may not be attained. If the amount is more than the aforesaid upper limit, the effect may not increase correspondingly. In askin cosmetic composition with a major purpose of whitening, the present compound is incorporated preferably in an amount of from 0.01 to 5 wt % based on the weight of the composition. 
     In the present skin cosmetic composition, any known substances can be incorporated in addition to the aforesaid present compounds, for example, those conventionally used in a skin cosmetic composition such as fats and oils, pigments, surfactants, humectants, UV absorbers, anti-inflammatories, fungicides, antiseptics, and colorants; β-adrenaline action stimulants, such as butopamine and isoproterenol; α2-adrenaline action depressants such as yohimbine and ergotoxine; xanthine derivatives, such as theophylline and caffeine; bipyridine derivatives, such as milrinone and amrinone; and those which suppress or prevent obesity, such as raspberry ketone and zingerone. 
     In the present food or drink composition, any known substances can be incorporated in addition to the aforesaid present compounds, for example, those conventionally used in a food or drink composition, such as saccharides, perfumes, emulsifiers, milk products, proteins, stabilizers, colorants, acidulants, fats and oils, cereals, eggs, gum base; xanthine derivative such as caffeine; hydroxy citric acid; and those which suppress or prevent obesity, such as capsaicin, raspberry ketone, zingerone and synephrine. 
     The present lypolysis promoter can be incorporated in food, oral drugs, or skin cosmetics in any dosage form. For example, the skin cosmetics for reducing weight may be of a form of cream, milky lotion, gel, stick, sheet, cataplasm, powder, liquid or granule to be applied to skin, for bathing, or for washing. The food or drink composition may be incorporated in chewing gum, chocolate, candies, gummy jellies, soup, ice cream, noodles, or bakery products. 
     EXAMPLES 
     The present invention will be explained in details with reference to the following Examples and Comparative Examples. Efficacy of lypolysis promotion was evaluated in the lypolysis test and in the subcutaneous fat decomposition test described below. Commercially available raspberry ketone and zingerone were used. 
     Preparation Example 1 
     4-(3′,4′-dihydroxyphenyl)-butane-2-one 
     Zingerone, [4-(3′-methoxy-4′-hydroxyphenyl)-butane-2-one], was dissolved in dichloromethane, to which 1.0 mol/l boron tribromide solution in dichloromethane was then added at −30° C. While stirring, the reaction mixture was gradually heated to a room temperature and allowed to react for further2 hours at the temperature. After adding ice water to the reaction mixture, an aqueous 2% sodium hydrogen carbonate solution was added. Then, the reaction mixture was extracted with ethyl acetate, and the ethyl acetate layer thus obtained was dried with anhydrous magnesium sulfate. Then, the ethyl acetate layer was vacuum condensed to obtain brown oil which was purified by silica gel chromatography, using a mixture of hexane/ethyl acetate =7/3 as a developing solvent. The white crystals thus obtained had almost no taste and no odor. Chemical structure was confirmed to be 4-(3′,4′-dihydroxyphenyl)-butane-2-one by GC-MS and NMR (see  FIGS. 1 and 2 ). 
     Preparation Example 2 
     4-(3′,4′-dihydroxyphenyl)-butane-2-ol 
     The procedures in preparation Example 1 were repeated except that use was made of 4-(3′-methoxy-4′-hydroxyphenyl)-butane-2-ol obtained by reducing zingerone with sodium borohydride, instead of zingerone. The white crystals thus obtained had almost no taste and no odor. Chemical structure was confirmed to be 4-(3′,4′-dihydroxyphenyl)-butane-2-ol by GC-MS and NMR (see  FIGS. 3 ,  4  and  5 ). 
     Method for Determination of Lypolytic Activity 
     Free fat cells were prepared from epididymis fat tissues of Wister male rats (body weight, 150 to 200 g) using a collagenase solution, according to Rodbell&#39;s method (M. Rodbell, J.Biol.Chem., 239,375(1964)). The cells were added to a Hanks&#39; balanced salt solution containing 0.05 μg/ml of bovine serum albumin, 0.05 μg/ml of norepinephrine and 100/μg/ml of the present compound to be tested, and then allowed to react at 37° C. for 1 hour. Liberated fatty acids were extracted and quantitated with a copper reagent and a color developing reagent. Lypolytic activity was calculated according to the following equation.
 
Lypolytic activity (%)=[ A/B ]×100,
 
wherein
         A: amount of fatty acids in a sample solution, and   B: that in a control solution without present compound.       

     Subcutaneous Fat Decomposition Test 
     Wister rats (male, 7 to 9 weeks old) were shaved at their bellies. Then, the belly cortex was enucleated together with subcutaneous fat tissues and mounted on Franz-type diffusion cells with a diameter of 2 cm. The keratin held in the upper cell was uniformly coated with 0.5 g of the present skin cosmetic composition and the lower cell for the subcutaneous tissue side was filled with a phosphate-buffer saline at pH 7.2. After keeping the cell at 37° C. for 6 hours, an aliquot of the buffer solution was taken out from the lower cell and glycerol liberated in the solution was quantitated by an enzyme method with F-kit Glycerol, ex Beringer Manheim Co. For each sample, the quantification was repeated five times and data were averaged. 
     Example 1 and Comparative Example 1 
     The lypolytic activity was determined on the present lypolysis promoter and raspberry ketone for comparison. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                   
                 TABLE 1 
               
               
                   
                   
               
               
                   
                   
                 degree of lypolysis 
               
               
                   
                   
                 promotion 
               
               
                   
                 Lypolysis promoter 
                 (%) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 Example 1 
                 4-(3′,4′- 
                 194.3 +/− 12.4 
               
               
                   
                 dihydroxyphenyl)- 
                 p &lt; 0.001 
               
               
                   
                 butane-2-one 
               
               
                 Comparative Example 1 
                 Raspberry ketone 
                 142.0 +/− 6.8  
               
               
                   
                   
                 p &lt; 0.01 
               
               
                   
               
             
          
         
       
     
     The present lypolysis promoter of Example 1 showed significantly higher lypolytic activity, compared with raspberry ketone of Comparative Example 1 
     Examples 2, 3 and Comparative Examples 2 and 3 (Gel Type Skin Cosmetic Composition for Reducing Weight) 
     Parts A and B were separately prepared by mixing and dissolving the substances in the amounts as shown in Table 2 in a conventional method. Then Part B was added to Part A with stirring to obtain a gel type skin cosmetic composition for reducing weight. 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                   
                   
                   
                 Com- 
                 Com- 
               
               
                   
                   
                   
                 parative 
                 parative 
               
               
                 Part (wt %) 
                 Example 2 
                 Example 3 
                 Example 2 
                 Example 3 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Part A 
                   
                   
                   
                   
               
               
                 4-(3′,4′-dihydroxy 
                 1.0 
                 3.0 
                 — 
                 — 
               
               
                 phenyl)-butane-2-one 
               
               
                 Raspberry ketone 
                 — 
                 — 
                 1.0 
                 — 
               
               
                 Glycerol 
                 10.0 
                 10.0 
                 10.0 
                 10.0 
               
               
                 Carboxyvinyl polymer 
                 0.3 
                 0.3 
                 0.3 
                 0.3 
               
               
                 Disodium edetate 
                 0.1 
                 0.1 
                 0.1 
                 0.1 
               
               
                 Purified water 
                 balance 
                 balance 
                 balance 
                 balance 
               
               
                 Diisopropanolamine 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
               
               
                 Squalane 
                 10.0 
                 10.0 
                 10.0 
                 10.0 
               
               
                 Part B 
               
               
                 Polyoxyethylene (60) 
                 0.8 
                 0.8 
                 0.8 
                 0.8 
               
               
                 hydrogenated castor oil 
               
               
                 Carrageenan 
                 3.0 
                 3.0 
                 3.0 
                 3.0 
               
               
                 Xanthan gum 
                 3.0 
                 3.0 
                 3.0 
                 3.0 
               
               
                 Polyvinylalcohol 
                 2.0 
                 2.0 
                 2.0 
                 2.0 
               
               
                 Ethanol 
                 45.0 
                 45.0 
                 45.0 
                 45.0 
               
               
                 Menthol 
                 0.1 
                 0.1 
                 0.1 
                 0.1 
               
               
                 Perfume 
                 small 
                 small 
                 small 
                 small 
               
               
                   
                 quantity 
                 quantity 
                 quantity 
                 quantity 
               
               
                   
               
             
          
         
       
     
     The results of the subcutaneous fat decomposition test on the aforesaid gel type skin cosmetic compositions are as shown in Table 3. 
     
       
         
               
               
             
               
               
               
             
           
               
                   
                 TABLE 3 
               
               
                   
                   
               
               
                   
                 Liberated Glycerol 
               
               
                   
                 (μmol/ml) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Example 2 
                 174.5 
               
               
                   
                 Example 3 
                 191.6 
               
               
                   
                 Comparative Example 2 
                 132.4 
               
               
                   
                 Comparative Example 3 
                 60.8 
               
               
                   
                   
               
             
          
         
       
     
     Example 4 and Comparative Examples 4 and 5(In Vivo Test on Obesity by High-Calorie Diet) 
     Each seven ICR mice per group (female, 5-week old at the beginning of the test, average body weight of 31 g) were bred for 3 weeks with high-calorie diet (Comparative Example 4) or high-calorie diet to which 4-(3′,4′-dihydroxyphenyl)-butane-2-one was added (Example 4) or a standard feed (solid standard feed MF, ex Oriental Yeast Co.) for a control group (Comparative Example 5). The mice were allowed to freely intake feed and drinking water, and weighed on the final day of the test period. Composition of the feed and the results are as shown in Tables 4 and 5, respectively. 
     
       
         
               
             
               
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Feed Composition 
               
             
          
           
               
                   
                   
                 Comparative 
               
               
                 Comparative 
                 Example 4 
                 Example 4 
               
             
          
           
               
                 Example 5 (Control) 
                   
                 wt % 
                 wt % 
               
               
                   
               
             
          
           
               
                 Solid standard feed 
                 Beef tallow 
                 40  
                 40 
               
               
                 MF, ex Oriental 
                 Corn starch 
                 10  
                 10 
               
               
                 Yeast Co. 
                 Granulated sugar 
                 9 
                 9 
               
               
                   
                 Mineral* 1   
                 4 
                 4 
               
               
                   
                 Vitamine* 2   
                 1 
                 1 
               
               
                   
                 Casein 
                 35  
                 36 
               
               
                   
                 4-(3′,4′- 
                 1 
                 — 
               
               
                   
                 dihydroxyphenyl)- 
               
               
                   
                 butane-2-one 
               
               
                   
               
               
                 * 1 AIN mineral mix, ex ICN Co. 
               
               
                 * 2 AIN vitamin mix, 
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Results 
               
             
          
           
               
                   
                 Comparative 
                   
                   
               
               
                   
                 Example 5 
                   
                 Comparative 
               
               
                   
                 (Control) 
                 Example 4 
                 Example 4 
               
               
                   
                   
               
             
          
           
               
                 Average weight (g) 
                 38.1 
                 38.4 
                 43.2 
               
               
                 Weight increment (g) 
                 7.1 
                 7.4 
                 12.2 
               
               
                   
               
             
          
         
       
     
     Example 5 (Melanogenesis Inhibition Test) 
     B16 melanoma cells were inoculated in a 12 wells-plastic plate at a concentration of 2×10 4 /well on MEM (Minimum Essential Medium). After 24 hours, the medium was changed to a Theophylline containing medium to which 4-(3′,4′-dihydroxyphenyl)-butane-2-one was added in the concentrations shown in Table 6. The cells were cultured for 72 hours and then treated with 10% TCA and ethanol/diethylether (=1/1). After counting the number of the cells, the cells were dissolved in an aqueous 1 mol/l sodium hydroxide solution containing dimethylsulfoxide in a concentration of 10%. Optical density at 475 nm (OD475 ) of the solution thus obtained was measured. The amount of the produced melanin per cell was calculated from OD475 and expressed in percentage relative to the amount observed in the blank sample where cells were cultured on a medium which did not contain 4-(3′,4′-dihydroxyphenyl)-butane-2-one. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 6 
               
               
                   
               
               
                 4-(3′,4′- 
                   
                   
               
               
                 dihydroxyphenyl)- 
                   
                   
               
               
                 butane-2-one 
                   
                   
               
               
                 concentration 
                 Amount of melanin per 
                   
               
               
                 (μg/l) 
                 cell (%) 
                 Standard deviation 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 0 
                 100 
                 11.2 
               
               
                 1 
                 97.4 
                 3.4 
               
               
                 3 
                 77.2 
                 2.2 
               
               
                 10 
                 62.7 
                 2.5 
               
               
                 30 
                 2.2 
                 0.3 
               
               
                   
               
             
          
         
       
     
     There was no difference among the numbers of the cells in the aforesaid concentration range of the present compound. 
     Example 6 (Anti-Oxidation Test) 
     To 1 ml of methyl linolate, 0.005g of the present compound was added, which was then irradiated with UV light with a UV light irradiation apparatus, M-DMR, ex Toshiba Co.. The amount of peroxides formed by oxidation of methyl linolate was determined according to a method described in Akasaka et al., Bioscience/Biotechnology/Biochemistry, Vol. 58, 396(1994), using diphenyl-1-pyrenylphosphine as a fluorescent labeling agent. Comparative test on raspberry ketone was made in the same manner as above. The results are as shown in Table 7. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                   
                 TABLE 7 
               
               
                   
                   
               
               
                   
                 UVB 10 (J/cm 2 ) 
                 UVB 30 (J/cm 2 ) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 Raspberry ketone 
                 30.2 
                 42.5 
               
               
                 4-(3′,4′-dihydroxyphenyl)- 
                 1.6 
                 3.7 
               
               
                 butane-2-one 
               
               
                   
               
             
          
         
       
     
     Examples 7 to 12 
     Skin lotion were prepared from the components as shown below and in Table 8. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Parts 
                 Amount (wt %) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 (A) 
                   
               
               
                   
                 Ethanol 
                 10.0 
               
               
                   
                 Monolauric acid 
                 5.0 
               
               
                   
                 polyoxyethylene(20) sorbitan 
               
               
                   
                 Dibutylhydroxytoluene 
                 0.01 
               
               
                   
                 Perfume 
                 0.05 
               
               
                   
                 (B) 
               
               
                   
                 The present compound described in Table 8 
               
               
                   
                 (C) 
               
               
                   
                 Glycerol 
                 5.0 
               
               
                   
                 Xanthan gum 
                 0.1 
               
               
                   
                 Hydroxyethylcellulose 
                 0.1 
               
               
                   
                 Purified water 
                 balance 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
             
           
               
                   
                 TABLE 8 
               
               
                   
                   
               
               
                   
                 Sample 
                 Amount (wt %) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Example 7 
                 4-(3′,4′- 
                 3.0 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-one 
               
               
                   
                 Example 8 
                 4-(3′,4′- 
                 0.5 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-one 
               
               
                   
                 Example 9 
                 4-(3′,4′- 
                 0.01 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-one 
               
               
                   
                 Example 10 
                 4-(3′,4′- 
                 3.0 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-ol 
               
               
                   
                 Example 11 
                 4-(3′,4′- 
                 0.5 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-ol 
               
               
                   
                 Example 12 
                 4-(3′,4′- 
                 0.01 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-ol 
               
               
                   
                   
               
             
          
         
       
     
     Preparation Method 
     Part B was homogeneously dissolved in Part C, to which Part A was added with stirring to be dispersed homogeneously. The product thus obtained was poured in a container. 
     Examples 13 to 18 (Skin Cream) 
     Skin creams were prepared from the components shown below and in Table 9. 
     
       
         
               
               
               
             
               
               
               
             
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Part 
                 Amount (wt %) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 (A) 
                   
               
               
                   
                 Glycerol monostearate 
                 2.0 
               
               
                   
                 Beeswax 
                 1.0 
               
               
                   
                 Monooleic acid polyoxyethylene(6) 
                 1.0 
               
               
                   
                 sorbitan 
               
               
                   
                 Vaseline 
                 4.0 
               
               
                   
                 Liquid petrolatum 
                 12.0 
               
               
                   
                 (B) 
               
             
          
           
               
                   
                 The present compound described in Table 9 
               
             
          
           
               
                   
                 (C) 
                   
               
               
                   
                 Sodium N-stearoyl-L-glutamate 
                 5.0 
               
               
                   
                 Carrageenan 
                 0.3 
               
               
                   
                 Methylparaben 
                 0.1 
               
               
                   
                 Purified water 
                 balance 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
             
           
               
                   
                 TABLE 9 
               
               
                   
                   
               
               
                   
                 Sample 
                 Amount (wt %) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Example 13 
                 4-(3′,4′- 
                 3.0 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-one 
               
               
                   
                 Example 14 
                 4-(3′,4′- 
                 0.5 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-one 
               
               
                   
                 Example 15 
                 4-(3′,4′- 
                 0.01 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-one 
               
               
                   
                 Example 16 
                 4-(3′,4′- 
                 3.0 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-ol 
               
               
                   
                 Example 17 
                 4-(3′,4′- 
                 0.5 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-ol 
               
               
                   
                 Example 18 
                 4-(3′,4′- 
                 0.01 
               
               
                   
                   
                 dihydroxyphenyl)- 
               
               
                   
                   
                 butane-2-ol 
               
               
                   
                   
               
             
          
         
       
     
     Preparation Method 
     Part A and a mixture of Components B and C were separately heated to 80° C. to make homogeneous solutions. Then, Part A was added to the mixture of Parts B and C and emulsified. The emulsion thus obtained was cooled to 30° C. with stirring. 
     Example 19 (Chewing Gum) 
     Chewing gum with the following composition was prepared. 
     
       
         
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 wt % 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Gum base 
                 20 
               
               
                   
                 Maltitol 
                 73.5 
               
               
                   
                 Reduced glutinous starch syrup 
                 4 
               
               
                   
                 Apple flavor 
                 0.5 
               
               
                   
                 Garcinia Cambogia Extract* 1   
                 1 
               
               
                   
                 4-(3′,4′-dihydroxyphenyl)-butane-2-one 
                 1 
               
               
                   
                   
               
               
                   
                 * 1 Garcinia Cambogia Extract, Citrimax HCA-500-F (trade name), ex Interhealth Nutraceutical Inc., containing 47.5% of hydroxycitric acid (HCA). 
               
             
          
         
       
     
     Example 20 (Sweet Tablets) 
     Sweet tablets with the following composition was prepared. 
     
       
         
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 wt % 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Sorbitol 
                 72.9 
               
               
                   
                 Sucrose ester of fatty acid 
                 4 
               
               
                   
                 Raspberry flavor 
                 0.1 
               
               
                   
                 Garcinia Cambogia Extract 
                 3 
               
               
                   
                 4-(3′,4′-dihydroxyphenyl)-butane-2-one 
                 10