Abstract:
Disclosed is a system and method of digesting raw animal waste using diffusers to force circulation and aerate a digesting lagoon. Several lagoons may be used in series to treat raw waste and different variations may be employed to successively digest raw waste. Aeration with slightly ozonated air facilitates a blend of aerobic and anaerobic microbes to quickly and completely digest large amounts of raw animal waste.

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application is a divisional of U.S. patent application Ser. No. 10/265,518, entitled “Waste Stream Digestion System,” filed Oct. 3, 2002, now U.S. Pat No. 6,743,357 by Jimmie A. Keeton, Jr., which was based upon and claims the benefit of U.S. Provisional Application Ser. No. 60/327,101 entitled “Waste Stream Digestion System,” filed Oct. 3, 2001 by Jimmie A. Keeton, the entire contents of which is hereby incorporated by reference for all it discloses and teaches. 
    
    
     BACKGROUND OF INVENTION 
     a. Field of the Invention 
     The present invention pertains generally to aerated digestion systems and specifically to aerated effluent processing systems with microbes. 
     b. Description of the Background 
     Anaerobic animal waste treatment systems are generally characterized by systems with liners or covered lagoons designed to mitigate smell and odor problems. Other systems include large biogas digesters. Anaerobic processes are very slow and rely on microbial decomposition reactions and processes that break down waste with no oxygen present. In addition, the anaerobic processes generate strong, unpleasant odors. Due to the slow process of digestion, there may be a rapid buildup of solids in the digestion ponds that must be removed and disposed of later. Further, anaerobic digestion does not remove salt content from runoff water. 
     Anaerobic systems are expensive to construct and may require a large capital investment to treat the effluent from a typical dairy or other farm operation. Often, covers are fitted to an anaerobic lagoon to capture and dispose of odorous gases such as H 2 S and ammonia. Such covers are expensive and prone to maintenance. 
     It would therefore be valuable to provide a system that processes waste quickly and efficiently, with a minimum of odor and as completely as possible. Further, it would be valuable to be able to process solid waste quickly enough that solid waste does not build up. Further, it would be valuable to provide a system that minimizes both the capital and recurring costs. 
     SUMMARY OF THE INVENTION 
     The present invention overcomes the disadvantages and limitations of the prior art by providing an aeration system coupled with a specific regimen of microbes that efficiently and effectively break down solid and liquid waste. The aeration system may function by aerating the waste from the bottom using a laminar column of fine bubbles to most efficiently circulate the waste and to maximize the oxygen transfer. The aeration system may further comprise an ozone generator and/or an oxygen generator to increase the oxygen transfer to the liquid. 
     Additionally, circulators in a lagoon may further enhance the movement of the effluent in a horizontal fashion. Such circulation prevents solids from collecting in various areas of a lagoon and maximizes the decomposition of solid matter. 
     Specialized microbes are added to the water that efficiently and effectively decompose the waste matter. The microbes are a blend of aerobic and facultative anaerobic microbes. Additionally, a blend of enzymes is added that increase the digestion rates of the waste matter. 
     The present invention may therefore comprise a method for the digestion of biological wastes comprising: introducing the waste into a lagoon; adding a blend of microbes to the lagoon comprising at least one of the following:  Bacillus, Saccharomyces,  and  Thiobacillus;  aerating the lagoon with a plurality of aerators located on the bottom of the lagoon, the aerator being adapted to produce a laminar column of bubbles; the aerators being supplied by a compressed air supply that has been enriched with ozone. 
     The present invention may further comprise a method for digesting biological wastes comprising: introducing the wastes into a first water filled lagoon; adding a blend of microbes to the first water filled lagoon, the blend comprising at least one of the following:  Bacillus, Saccharomyces,  and  Thiobacillus;  aerating the first water filled lagoon with a plurality of aerators dispersed on the bottom of the first water filled lagoon, the aerators adapted to produce a laminar column of bubbles, the aerators being supplied by a first compressed air supply; circulating the first water filled lagoon with at least one circulator adapted to cause the first water filled lagoon to circulate; sending a portion of the water filled lagoon to a second water filled lagoon; aerating the second water filled lagoon with a plurality of aerators dispersed on the bottom of the second water filled lagoon, the aerators being supplied by a second compressed air supply; and circulating the second water filled lagoon with at least one circulator adapted to cause the first water filled lagoon to circulate. 
     The advantages of the present invention are that a substantially less amount of energy is required to decompose solid biological wastes than with existing systems, due to the laminar flow diffusers, the large degree of circulation, and the selection of specific microbes. Further, the present invention has the ability to completely decompose solid wastes to the point that solid waste build up in the lagoons is virtually eliminated. Any solids left in the effluent after processing are held in suspension and deliver nutrients when used as fertilizer. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       In the drawings, 
         FIG. 1  is an illustration of an embodiment of the present invention comprising a two-lagoon digestion system. 
         FIG. 2  is an illustration a cross-section of a typical lagoon of the embodiment of the present invention of FIG.  1 . 
         FIG. 3  is an illustration of a top view of an embodiment of the present invention wherein four circular lagoons are used in series to treat effluent. 
         FIG. 4  is an illustration of a top view of lagoon of the embodiment of the present invention of  FIG. 3  showing the aeration system. 
         FIG. 5  is an illustration of a section view of a lagoon of the embodiment of the present invention of FIG.  3 . 
         FIG. 6  is an illustration of an embodiment of the present invention comprising three lagoons. 
         FIG. 7  is an illustration of an embodiment of the present invention comprising two long, narrow lagoons. 
         FIG. 8  illustrates the cation decrease with an embodiment of the inventive waste processing system. 
         FIG. 9  illustrates the decrease in electrical conductivity of the water with three experimental embodiments of the present invention. 
         FIG. 10  illustrates the tons of salt removed per acre-foot with three experimental embodiments of the present invention. 
         FIG. 11  illustrates the TKN and ammonia nitrogen decrease of three experimental embodiments of the present invention. 
         FIG. 12  illustrates the percent decrease in TKN and ammonium nitrogen of three experimental embodiments of the present invention. 
         FIG. 13  illustrates the percent nitrogen in the product water for three experimental embodiments of the present invention. 
         FIG. 14  illustrates the decrease in biological oxygen demand for three experimental embodiments of the present invention. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       FIG. 1  is an illustration of an embodiment  100  of the present invention comprising a two lagoon digestion system. Effluent is introduced by arrow  102  into primary lagoon  104 . Primary lagoon  104  is outfitted with several air diffusers  106  that are fed by manifold  108  that is supplied by air supply  110 . Primary lagoon  104  is further outfitted with surface circulators  112 . Secondary lagoon  114  is fed by the overflow of primary lagoon  104 , and has a set of air diffusers  116  connected to manifold  108 . Secondary lagoon  114  is further outfitted with surface circulators  118 . A blow off valve  120  is part of manifold  108 . 
     The principle of operation of embodiment  100  is to introduce raw or filtered effluent, such as from a dairy, into a lagoon for digestion. The lagoon is inoculated with microbes and has a source of oxygen. Several bottom mounted fine bubble diffusers introduce oxygen into the lagoon. The diffusers serve an additional purpose of forced vertical circulation. Surface circulators force floating solids back into the lagoon for further digestion as well as circulate the lagoon in the horizontal plane. The diffusers are adapted to produce laminar columns of fine bubbles, which entrain large amounts of liquid and prevent build up of solid wastes in the lagoon. Further, the laminar columns of bubbles are the most efficient method of transferring oxygen into the liquid. Embodiment  100 , through the high circulation and constant movement of the liquid, may fully digest solid waste introduced into the system. 
     In embodiment  100 , the effluent is digested in two stages. The primary lagoon  104  performs much of the active digestion and decomposes much of the solid mass. The secondary lagoon  114  completes the digestion. The primary lagoon  104 , due to the intensity of the digestion process at the early stages, may require much more aeration than secondary lagoon  114 . By having multiple lagoons, the aeration requirements are less at each successive stage, thus requiring less energy for oxygenation. In general, the retention time of the primary lagoon  104  is in excess of 15 days. 
     In general, the lagoons are between 15 and 20 feet deep. For lagoons less than 15 feet deep, the oxygen transfer of the aerators begins to be substantially less efficient. For lagoons deeper than 20 feet, a regenerative type blower may not provide enough efficiency and a more expensive piston pump or other air pump may be needed. 
     The air system typically provides a minimum of 2.5 pounds of oxygen per pound of biological oxygen demand. The diffusers typically circulate up to 2,500 gallons per minute of water to the surface of the lagoons through the entrainment of water in the rising laminar bubble column. The airlift principle of the entrained water is the most efficient method of oxygen transfer as well as the main mechanism whereby solids are kept in suspension and anaerobic conditions are prohibited from forming. A typical system may have ozone enrichment at the rate of 12 grams to 20 grams per hour, with an airflow per diffuser of 1.5 to 2.5 cubic feet per minute 
     Effluent may be introduced into the primary lagoon  104  in either the raw or filtered state. The raw state may include all solid waste plus hay, straw, and other material commonly used in cow stalls or other agriculture environment. The effluent generally contains urine plus water used in cleaning the stalls. The effluent may also contain additional material, such as twine, rope, and other material that may or may not be biodegradable. 
     The primary lagoon  104  and secondary lagoon  114  may be of any shape, including rectangular, square, circular, or other shape. The layout and placement of aerators  106  and circulators  112  are selected to minimize dead spots or areas where solid waste may not circulate well. The layout and arrangement of the primary lagoon  104  and the secondary lagoon  114  may be such that the secondary lagoon  114  may be located at a lower elevation than the primary lagoon  104 , such that the output from the primary lagoon  104  may travel downhill to the secondary lagoon  114 . In some embodiments, a pump may be used to transfer effluent from one lagoon to a second lagoon. 
     The diffusers  106  and  116  are connected to manifold  108 . Manifold  108  in the present embodiment delivers air to all the diffusers in the system as supplied by air supply  110 . In alternative embodiments, a plurality of air supplies  110  may feed several manifolds, such as a separate manifold for each lagoon, or one air supply and manifold for one side of a lagoon and a second air supply and manifold for the opposite side of the lagoon. 
     The surface circulators  112  and  118  serve to slowly circulate the lagoon in the horizontal direction and may act to reintroduce floating solid waste back into the liquid for further digestion. The circulators may be aerating surface circulators, paddlewheels, submerged propellers or any other device adapted to move water in a horizontal fashion. In some cases, such as the aerating surface circulators or paddlewheels, the circulators may help reintroduce floating debris and solid waste back into the lagoon for further digestion. When solid waste is floating on the surface of the lagoon, very little digestion is being accomplished since much of the surface area is not in contact with the liquid borne microbes. Further, the waste may undergo mechanical disintegration from the tumbling circulation of the aerated lagoon. 
     The air supply  110  may comprise a regenerative blower. In some embodiments, the air supply may be a multistage regenerative blower, a piston compressor, or other types of air compressors and/or blowers. Further, some embodiments may include an oxygen generator and/or an ozone generator as part of the air supply  110  modules. Oxygen and ozone generators may be used to increase the oxygen transferred to the liquid and thereby increasing the microbe activity. The air supply may be powered by electrical line from a power grid, a diesel engine, solar power, or other power sources. 
     The air supply  110  may include a cooling system for cooling the air as it enters the manifold  108 . Further, a cooling system may be employed to cool the compressors or blowers. A cooling system may use effluent from a lagoon as part of the heat transfer media. In some embodiments wherein several air supplies  110  are employed, an air supply for a first lagoon may include oxygen generators and/or ozone generators, and a second air supply for a second lagoon may not include the oxygen and/or ozone generators. Such embodiments may use an oxygen generator for a primary lagoon and no oxygen generator for a secondary lagoon. 
     The treated water that exits the secondary lagoon  114  may be used to irrigate fields. Due to the nature of the system, small particles may be in suspension in the outflow water. The salt content of the treated effluent may have 3.8 tons of salt removed per acre foot of water. The Total Kjeldahl Nitrogen (TKN) reduction may be on the order of 95%, where approximately 30% of all nitrogen in the processed water is in the form of protein and amino acids that can be utilized by soil organisms and plant material when used for irrigation. Because the biological oxygen demand of the treated water is reduced so much, soils irrigated with the treated effluent may remain aerobic while still providing a more usable form of organic matter that includes both complex and simple sugars. Virtually all nitrates are removed from the treated water by the process. Weed seeds, which can cause problems for farming operations, are digested by the process and are not found in the treated water. 
       FIG. 2  is an illustration an embodiment  200  of a cross-section of a typical lagoon  202 . A manifold  204  is connected to a pipe  206  that is in communication with a diffuser  208 . The bottom-mounted diffuser  208  is adapted to produce a laminar column of fine bubbles  210 . 
     The lagoon  202  may be approximately 10 to 20 feet deep and may have a clay bottom or other construction to prevent leakage. In some cases, the lagoon  202  may have a plastic or rubber liner to prevent leaking. The lagoon may be 50-300 feet wide, depending on the application. Other sizes of lagoons may be used without deviating from the spirit of the present invention. 
     The manifold  204  may be mounted above ground as in  FIG. 2 , or may be mounted below ground. The manifold may be constructed of PVC piping, steel piping, or any other type of pipe as desired. In some cases, the compressed air is very hot and causes problems with the manifold piping. 
     The pipe  206  is preferably a self weighted tubing, with a density substantially higher than water, such that the tubing may lay on the bottom surface of the lagoon. In some embodiments, the pipe  206  may be a rigid or semi-rigid tubing and may need to be weighted down with bricks or other objects to prevent the pipe  206  from moving when inflated. Pipe  206  may include a flow control or meter so that the individual diffusers may be adjusted to deliver a laminar column of bubbles. 
     The diffuser  208  may be mounted on the bottom of the lagoon  202 , which has several advantages. For example, the further the bubbles have to travel, the more time the oxygen has to transfer from the bubble to the effluent. Further, the rising column of bubbles is a very efficient method of inducing circulation, and the bottom mounting ensures that the dead space where water does not circulate is minimized. 
     The diffuser  208  may be a large (6 to 12 inch) expanding membrane fine bubble diffuser. The size of the diffuser  208  may be determined by the application. For example, for a shallower lagoon, a smaller diffuser may be used. A smaller diffuser may be used in larger lagoons where the diffusers are placed closer together. In some cases, a smaller diffuser may be used in the middle of the lagoon and a large diffuser may be used in the corner of a lagoon. In some embodiments, an adjustable flow control may be used to individually control the amount of air flowing to a particular diffuser. A flow control may be adjusted to decrease flow on a diffuser that is generating turbulent or slug flow. A flow control may be adjusted to increase flow to a diffuser where solid waste is building up near the diffuser. In some embodiments, the oxygen requirements for a secondary lagoon may be less than that of a primary lagoon, therefore the diffusers in the secondary lagoon may be restricted to deliver less air than the diffusers of the primary lagoon. In some embodiments, the pipe  206  and the diffuser  208  may be buried in the lagoon bottom. 
     The circulation patterns produced by the diffusers are such that strong vertical currents may be produced in the lagoon especially as the result of the laminar flow of bubbles produced by the diffusers that increase such vertical circulation substantially. The strength of the currents keeps solid wastes moving and constantly being decomposed. Sufficient strength of the currents keeps the wastes in suspension so that the microbes present in the effluent may effectively and completely decompose and disintegrate the solid matter. 
     The microbes in the lagoon may be  Bacillus subtilis, Bacillus licheniformis . The formulation of the microbes is about 65%  Bacillus subtilis , 25%  Bacillus licheniformis , and the balance an enzyme formulation of alpha-amylase of 1-3%, beta-gluconase of 1-3%, protease of 1-3%, lipase of 1-3%, esterase of 1-3%, hemi-cellulase of 5%, wheat bran at 12 ounces per pound, sodium chloride at 1 to 2% as a preservative. The formulation may be inoculated into the lagoons at strength of 4 billion CFU/gram of material and in the amounts of 5 to 10 lbs per surface acre. The frequency of inoculation may be approximately biweekly. The amounts of each inoculant may be varied based on the conditions of the digestion system. 
     Deoxygenase enzyme is added in addition to the microbe and enzyme formulation above. 
       FIG. 3  is an illustration of a top view of an embodiment  300  of the present invention wherein four lagoons are used in series to treat effluent. The effluent is introduced into the primary lagoon  302  through the inlet  304 . A circulator  306  provides horizontal circulation for primary lagoon  302 . The outlet  308  connects primary lagoon  302  to secondary lagoon  310 . A circulator  312  provides horizontal circulation for secondary lagoon  312 . The outlet  314  connects secondary lagoon  310  to tertiary lagoon  316 . A circulator  318  provides horizontal circulation of tertiary lagoon  316 . The outlet  320  connects tertiary lagoon  316  to final lagoon  322 . A circulator  324  provides horizontal circulation of final lagoon  322 . The product water exits the system through outlet  326 . 
     Primary lagoon  302  has a drain  328  that is connected to sludge pit  330 . Secondary lagoon  310  has a drain  332  connected to sludge pit  330 . Tertiary lagoon  316  has a drain  334  connected to sludge pit  330 , and final lagoon  322  has a drain  336  connected to sludge pit  330 . Sludge pit  330  has a drain  338  and a return system  340  to pump sludge into primary lagoon  302 . 
     The embodiment  300  is a four lagoon embodiment wherein each circular lagoon is highly aerated and constantly circulated to efficiently and effectively digest raw and filtered effluent waste. Each lagoon may be outfitted with a plurality of bottom mounted fine bubble aerators and inoculated with a special blend of microbes. The aeration system of a typical lagoon will be described hereinafter and may provide a vertical circulation and constant motion of the effluent. The drain system in the center of each lagoon may provide a mechanism whereby any solids present in a lagoon may be drained into sludge pit  338  and pumped back into primary lagoon  302 . In this manner, the solids may be recycled through the embodiment  300  to completely digest the solids. 
     Lagoons  302 ,  310 ,  316 , and  322  may be approximately 245 feet in diameter for a system designed to handle the daily raw waste of a dairy of about 4000 head of cattle. Approximately 75 tons of raw waste may be introduced into inlet  304  per day. The present invention may also be adapted to be used for chicken waste, pig waste, human waste, and other biological wastes. 
     The circulators  306 ,  312 ,  318 , and  324  may be surface mounted aspirating circulators, surface mounted paddle wheel circulators, subsurface circulators, or any other type of device adapted to move the effluent in a horizontal fashion. 
       FIG. 4  is an illustration of a top view of lagoon  302  showing the aeration system in lagoon  302 . The inlet  304  and outlet  308  are shown in the present figure. Drain  328  is also shown in the present figure. An air source  402  is connected to an air manifold  404  that encircles the lagoon  302 . A diffuser  406  is connected to manifold  404  through a self-weighted pipe  408 . Several diffusers are located throughout the lagoon  302  and are all in communication with the manifold  404 . 
     The air source  402  may contain a high volume, low pressure air source. In some cases, a regenerative blower or other type of compressor may be used and may provide up to 10 psi pressure and 1.5 to 2.5 cubic feet per minute per diffuser. The air source  402  may have an additional oxygen generator and/or an ozone generator to enhance the oxygenation of the effluent. The air source  402  may include a cooling system for cooling the air produced by the air source. Such a cooling system may use effluent as a cooling medium. The manifold  404  may be buried around the edge of lagoon  302  or may be mounted above ground. The manifold  404  may be on the order of 4 inches in diameter. The air source  402  may supply air to all four lagoons  302 ,  310 ,  316 , and  322  simultaneously. The air source  402  may be designed to run continuously. 
     Each lagoon  302 ,  310 ,  316  and  322  may be outfitted with a similar aeration system as in the present figure. However, secondary lagoon  310  may require fewer diffusers than primary lagoon  302 , tertiary lagoon  316  may require fewer diffusers than secondary lagoon  310 , and final lagoon  322  may require fewer diffusers than tertiary lagoon  316 . As the effluent is treated in each subsequent lagoon, the oxygen requirements are less and therefore fewer diffusers may be required. 
       FIG. 5  illustrates a section view of lagoon  302  and sludge pit  330 . The center drain  328  leads into sludge pit  330 , where a pump may be mounted in drain  338  and recycle sludge through the pipe  340 . The air manifold  404  is in communication with diffuser  406  through pipe  408 . 
     The sludge pit  330  may be fed with the sludge from all four lagoons  302 ,  310 ,  316 , and  324 . Valves not shown on each drain system may allow an operator to control the flow of the sludge to the sludge pit. Such valves may be manually or automatically actuated. 
     The diffuser  406  may be a fine bubble diffuser adapted to form a laminar column of air bubbles. Laminar bubble columns have been found to be the most efficient method for entraining water and forcing circulation. Because of the location of the diffuser  406  on the bottom of the lagoon, the maximum amount of circulation may be achieved. The circulation is important in the effectiveness of the present system, since the constant motion of solid wastes prohibits the wastes from collecting and building up in the lagoons. Further, the longest duration of contact is achieved between the air introduced by the diffuser  406  and the effluent in the lagoon. Additionally, since the laminar flow is slower than slug flow or turbulent flow, more time is allowed for the contact of the bubbles with the liquid. 
       FIG. 6  illustrates an embodiment  600  of the present invention comprising three lagoons of the approximate size of 300 by 150 feet. The lagoons are approximately 15 feet deep. Effluent enters by arrow  602  into the primary lagoon  604 , which feeds secondary lagoon  606 , which feeds tertiary lagoon  608 . The arrangement of surface circulators  610  and diffusers  612  is shown in the figure. 
     Embodiment  600  is designed to handle approximately 75 tons of unfiltered effluent per day. The lagoons are designed to process waste in a progressive fashion, with a majority of digestion taking place in the primary lagoon  604 , and less digestion taking place in each successive lagoon. 
     Primary lagoon  604  is outfitted with four surface circulators  610  and  35  diffusers  612 . Several of the diffusers are located in the comers of the lagoon to force circulation in the corners and prevent the stagnation and buildup of solid wastes. The surface circulators  610  are arranged to keep the horizontal circulation progressing throughout the lagoon  604 . 
     Secondary lagoon  606  requires less oxygenation and less circulation since most of the solid waste is decomposed in primary lagoon  604 . Therefore, only two surface circulators  610  and  18  diffusers  612  are required. 
     Tertiary lagoon  608  requires still less oxygenation than secondary lagoon. Therefore, only two surface circulators  610  and 9 diffusers  612  are required. 
       FIG. 7  illustrates an embodiment  700  of the present invention comprising two long, narrow lagoons of the approximate size 1000 by 120 feet. Effluent is introduced at arrow  702 , is processed by primary lagoon  704  and completed in secondary lagoon  706 . 
     Embodiment  700  may handle approximately 75 tons of unfiltered raw waste per day. The depth of the lagoons is about 15 feet. The lagoons may be oriented so that the flow of effluent is downhill from the primary lagoon  704  to the secondary lagoon  706 . 
     Primary. lagoon  704  is outfitted with eight surface circulators  708  and  74  diffusers  710 . Secondary lagoon  706  is outfitted with four surface circulators  708  and 46 diffusers  710 . 
     The foregoing description of the invention has been presented for purposes of illustration and description. It is not intended to be exhaustive or to limit the invention to the precise form disclosed, and other modifications and variations may be possible in light of the above teachings. The embodiment was chosen and described in order to best explain the principles of the invention and its practical application to thereby enable others skilled in the art to best utilize the invention in various embodiments and various modifications as are suited to the particular use contemplated. It is intended that the appended claims be construed to include other alternative embodiments of the invention except insofar as limited by the prior art. 
     EXPERIMENTAL RESULTS SEPTEMBER 2001 
     Experiments have been conducted using the inventive waste stream digestion system during September 2001 at California Dairies, Inc., of Tipton, Calif. 
     The results below were for a 1.43 acre secondary pond receiving approximately 410,000 gallons of influent from a primary digestion pond. The secondary pond had been outfitted with 74 Duraplate diffusers from Keeton Industries. The air supply was a 20 HP two stage regenerative blower producing air at a pressure of 240 inches of water. An ozone generation system producing 10 to 12 grams per hour of ozone was used downstream of the air source. The pond was approximately 10 feet deep. 
     The biological oxygen demand (BOD) of the influent was 3841 lbs per day. The transfer rate of oxygen was calculated at 5761 lbs of oxygen per day. 
     The results show that the above system has a transfer rate of 12.2 lbs of oxygen per horsepower per hour. This compares to traditional paddlewheel aeration where the maximum transfer rate is approximately 1.5 lbs of oxygen per horsepower per hour. 
     EXPERIMENTAL RESULTS JANUARY 2001 
     The January 2001 results of the Water Pure aerobic bioremediation of dairy waste include the analysis of three dairies, one with a solid separator. The analysis of all three dairy pond systems includes the determination of the Biological Oxygen Demand (BOD) and the Total Kjeldahl Nitrogen (TKN) for each sample taken. The results of these analyses have allowed the inventive method of bioremediation to be more easily explained and verified. 
     It is determined that the decrease in the Electrical Conductivity of the water (ECw) was mainly because of the calcium ion and magnesium ion forming precipitates as carbonates and phosphates. It is assumed that the decrease in the sodium and potassium ion was associated with the incorporation of these more soluble ions into the bodies of the organisms involved in the digestion of the organic matter in the waste. Another observed decrease in cation concentration is associated with the ammonium ion eliminated by the denitrification, reduction to nitrogen gas, N 2  discussed later.  FIG. 8  illustrates the cation decrease with three experimental embodiments of the inventive waste processing system. 
     The average decrease in the ECw is 44% corresponds to an average reduction of the over-all salt by 3.4 tons of salt per acre-foot of the digested product water. The restrictions placed on the number of dairy animals based on the salt output from each animal may be able to be reevaluated. This method of bioremediation if applied to dairy waste streams may provide a way to decrease groundwater contamination. The lower the ECw of the product water, the safer the product water becomes as an irrigation source. The agricultural operations at or near the dairies utilizing the inventive waste stream digestion system for aerobic digestion may be able to more safely continue the long-term use of this water while minimizing salt and extra nutrient in the irrigation water.  FIG. 9  illustrates the decrease in ECw with three experimental embodiments of the present invention while  FIG. 10  illustrates the tons of salt removed per acre-foot with the same experimental embodiments of the present invention. 
     Because of the control and monitoring of the product water nutrient output, which is part of the bioremediation program, nutrient contamination of ground water may be minimized. Because the overall nutrient load in the product water will be known, the farming operations may be able to eliminate unnecessary applications of other fertilizers. This can provide a cost savings in nutrients as well as a savings in the labor associated with the application of these nutrients. As the regulatory agencies require more control and documentation of all nutrients applied to farming operations, the utility of the use of this type of product water sources may become even more valuable. The utility has been proven so far in silage grown on fields using the product water, which has undetectable nitrate concentrations. The corn silage grown with this water has provided 30% higher tonnage of silage and 40% higher protein content. The uptake of the nitrogen of all sources as well as the uptake of micronutrients such as zinc and manganese has been observed. 
     The nitrogen sources in the undigested flush lanes solution contain a complex mixture of nitrogen compounds, which includes anything from proteins, individual amino acids, free ammonical nitrogen including either ammonia, and ammonium ion as well as urea and nitrate ion. The inventive process includes the digestion and denitrification of the original flush lane solution.  FIG. 11  illustrates the decrease in TKN and ammonia nitrogen of three experimental embodiments of the present invention. The Visser Dairy, which does not have a solid separator, is the most efficient system and has been in operation for the longest time. The digestion ponds at the Visser Dairy are deeper and have the longest retention time of any of the systems tested. 
     The denitrification of the ponds, the reduction of the more complex nitrogen compounds to nitrogen gas, N 2 , decreases the potential contamination of the groundwater. The 87% reduction in the Total Kjeldahl Nitrogen and the 35% reduction in the ammonical nitrogen at the Visser Dairy provide a measure of the efficiency of this process when ideal conditions are maintained. The DeHoog Dairy, which has very shallow ponds, still shows 66% efficiency in the reduction of the TKN and a 29% reduction of the ammonical nitrogen has no solid separator. The JCJ Dairy, which utilizes a solid separation system, shows a 40% TKN reduction and a 26% reduction of the ammonical nitrogen. The reduced efficiency at the JCJ operation may be associated with the ECw being 2.3 times as high in the digestion pond than at the other dairies. The product water at the JCJ Dairy is also reused in the flush lanes leading to the higher salt concentration in the digestion ponds. It should be noted that the product water from all three dairies continues to have undetectable concentrations of nitrate ion.  FIG. 12  illustrates the percent decrease in TKN and ammonium nitrogen of three embodiments of the present invention. 
     It should be noted that the product water still contains some complex nitrogen compounds such as amino acids. These amino acids have been shown to help to complex or chelate micronutrient metal ions and allow them to be more easily transferred across the root&#39;s membrane. In earlier tissue analysis of silage corn, it has been shown that both the zinc and manganese concentrations were greatly enhanced when the aerobically digested water was used for irrigation resulting in 40% higher protein content and 30% greater yield.  FIG. 13  illustrates the percent nitrogen in the product water for three experimental embodiments of the present invention. 
     The Biological Oxygen Demand, BOD, a measure of the organic matter in solution was determined for the flush lane solutions and the product water for each of the dairies. The results of these analyses are shown in FIG.  14 . It should be noted that each of the dairy operations shows fairly efficient BOD reduction, but as might be expected the Visser system is the most efficient and the JCJ the least efficient. The JCJ Dairy operation was still over 80% efficient in the digestion of the original organic matter in the pond solution. The digestion process depends on a number of factors only one of them being the salt concentration. The process is observed to be even more efficient during the warmer months. 
     As the organic matter is efficiently digested in the present invention, the BOD is decreased by as much as 95%. The organic matter that might have loaded the soil and decrease the available soil oxygen has been greatly reduced and the soil oxygen remains more available. With more availability of oxygen in the soil a much healthier soil environment can be maintained for the critically important aerobic soil organisms. If oxygen rich conditions are maintained in the soil eco-system, the critical balance of all organisms can not only exist but also thrive. Once these balanced conditions are established and maintained most soil born diseases are held in check by these healthy competing organisms. 
     SUMMARY OF EXPERIMENTAL RESULTS 
     The average 44% decrease in the Electrical Conductivity, EC, between the flush lanes and the product water, corresponds to a decrease of 3.4 tons of salt per in an acre-foot. This should allow the dairy to maintain more animal units at a particular dairy site if the present inventive method of aerobic digestion is maintained. 
     The continued efficient operation of the present inventive process is documented by the aerobic BOD reduction. An average of 90% reduction of the organic material suspended in the water from the flush lanes continues to be observed. 
     The denitrification, the conversion of the more complex nitrogen compounds to inert N 2  gas, shows an average efficiency of approximately 64% and as high as 87% reduction. The decrease in the total nitrogen minimizes the possibility of nitrate contamination of ground water when the product water is used for irrigation purposes. The nitrogen utilized by irrigated crops can further reduce the possibility of nitrate contamination of the ground water. 
     The average documented 90% decrease in the Biological Oxygen Demand, BOD, and the average lowering of the ECw by 44%, a decrease of 3.4 tons of salt per acre-foot of water, allows this product water to be used for irrigation without creating anaerobic or problem saline conditions in the soil.