Abstract:
Particulate crystalline therapeutic substances are formulated with stabilizers to enhance contact between the crystalline therapeutic substances and the mucosal membranes to provide extended therapeutic effect.

Description:
RELATIONSHIP TO OTHER APPLICATIONS 
     This is a continuation-in-part of application Ser. No. 08/366,841, filed Dec. 30, 1994, U.S. Pat. No. 5,628,981. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to improved formulations of poorly soluble crystalline therapeutic agents which are absorbed through mucous membranes. 
     2. Background of the Invention 
     There is a constant need in the pharmaceutical industry for improved pharmaceutical formations which enhance the efficacy of poorly soluble therapeutic agents. There is especially a need for formulations that (1) enhance the adsorption of poorly soluble therapeutic agents across mucous membranes or (2) extend the period of time that poorly soluble therapeutic agents useful for treating of a site have contact with the mucosa of the site. 
     Since most poorly soluble crystalline therapeutic agents do not inherently adhere to mucosal surfaces, there is a need to provide in such formulations, primary surface active stabilizers with appropriate mucoadhesive properties and secondary excipients that provide for an improvement in therapeutic efficacy as compared with existing products and formulations. Therefore, the physical-chemical properties of the therapeutic agents, and any desired surface active stabilizers and viscosity modifiers must be taken into consideration. 
     The identification of surface active stabilizers for use with small particles of crystaline poorly soluble drugs, wherein the surface active stabilizer has bioadhesive or mucoadhesive properties to mucous membranes, in particular, to the entire GI tract, has not been reported to date. The development of appropriate surface active stabilizers that will enable mucous membranes to be utilized to enhance bioavailability represents a difficult technical problem. 
     Bioadhesion is usually achieved by interaction of either a synthetic or natural polymeric substance with mucosal membranes. Such technology has been employed to enhance drug delivery by decreasing the transit time of a drug substance in the GI tract and hence promote an opportunity for enhanced absorption. Highly charged carboxylated polyanions are good candidates for use as bioadhesives in the GI tract. See, for example: Park, K. and Robinson, J. R., Bioadhesion: Polymers and Platforms for Oral-Controlled Drug Delivery; Method to Study Bioadhesion. Int. J. Pharm., 19, 107 (1984). The formation of a bioadhesive bond between a polymeric substance and the mucosal lining of the GI tract can be visualized as a two step process, i.e., initial contact between the two surfaces and the formation of secondary bonds due to non-covalent interactions. Bioadhesives specific for mucous membranes must interact with the mucus layer during attachment. Mucus, a general term for the heterogenous secretion found on the epithelial or endothelial surfaces of the mucous membranea, is made of the following components: glycoprotein macromolecules, inorganic salts, proteins, lipids and mucopolysaccharides. These glycoproteins typically consist of a protein core with carbohydrate side chains. This forms a network of mucus that is a continuous layer covering the muscous membrane. From a bioadhesive perspective, mucus consists of highly hydrated, crosslinked linear, flexible yet random coiled glycoprotein molecules with a net negative charge. Understanding the principles of bioadhesion is the basis for producing an improved formulation for poorly soluble therapeutic agent. Bioadhesion accounts for the interaction between a biological surface and a biomaterial substance. As noted previously, bioadhesive agents are usually polymeric substances that adhere to tissues by ionic or covalent bonds or by physical attachment. Several theories of bioadhesion have been published including electronic, adsorption, wetting, diffusion and fracture theories. Bioadhesives bind to membrane surfaces and are retained for variable periods of time. 
     The primary difficulty with previously reported mucoadhesive stabilizers is that they do not interact effectively with both the particles of the therapeutic agent and mucous membrane uniformly so that, e.g., both the upper and lower GI tract can be used as sites to increase bioavailability. The stabilizers used for this purpose must be adsorb sufficiently to the different regions of the GI tract. In practice, stabilizers tend to be adsorbed at some biological surfaces differentially than at others due to a variety of complex reasons. There is a need for formulations of particles of therapeutic agents that are adsorbed sufficiently over the entire mucous membrane. 
     It has now been discovered that particles of crystalline therapeutic agents modified by having a poloxamer absorbed on the surface thereof, can be adsorbed onto the mucosal surface, including the GI tract. 
     In another embodiment the present invention relates to improved formulations of therapeutic agents, including gastrointestinal therapeutic agents, wherein the formulations provide prolonged local contact of the therapeutic agents with the mucosal layer, including the gastrointestinal tract. 
     SUMMARY OF THE INVENTION 
     In accordance with the present invention, there is provided a therapeutic composition comprising: 
     from about 0.1% to about 45% w/v, and, preferably, from about 0.5% to about 15% w/v, of particles of a poorly water-soluble crystalline therapeutic agent having a weight average particle size of less than about 2,000 nm, more preferably, a weight average particle size of less than about 1,000 nm, and most preferably, a weight average particle size of less than about 400 nm; 
     from about 0.1% to about 10% w/v, and preferably of from about 0.25% to about 5% w/v of a poloxamer; and 
     water to make 100% w/v. 
     These compositions can be administered by any route that will result in the therapeeutic agent having contact with a mucous membrane. These routes include oral, rectal, nasal, occular, inhalation and vaginal. Preferred is oral and rectal because the compositions of the present invention will enable the thereapeutic agent to be adsorded across the mucous mebrane of the entire GI tract resulting in greatly enhance adsorption. 
     Secondary stabilizers may also be used in the therapeutic composition up to about 1% w/v, preferably up to about 0.2% w/v, and most preferably up to about 0.1% w/v. Secondary stabilizers include dioctylsulfosuccinate (DOSS) and sodium lauryl sulfate (SLS). 
     Other ingredients customarily used in pharmaceutical formulations may also be included, such as flavorants, colorants and preservatives to provide pharmaceutically acceptable and palatable formulations. 
     The poloxamers useful in the present invention have an average molecular weight of from about 1,000 to 15,000 daltons; preferred have an average molecular weight of about 5,000 to 15,000 daltons. 
     Poloxamers are polyethylene-polypropylene glycol block polymers containing ethylene oxide (PEO) and propylene oxide (PPO) moles according to the formula 
     
       
         (PEO) a −(PPO) b −(PEO) c . 
       
     
     Preferred are poloxamers wherein; 
     a is 46 to 128; 
     b is 16 to 67; and 
     c is 46 to 128. 
     More preferred are poloxamers wherein; 
     a is 46, 52, 62, 75, 97, 98, 122 and 128; 
     b is 16, 30, 35, 39, 47, 54 and 67; and 
     c is 46, 52, 62, 75, 97, 98, 122 and 128. 
     Table 1 shows the various poloxamers by manufacturer-designated number. 
     
       
         
               
             
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Molecular Weights of Poloxamers 
               
             
          
           
               
                   
                 Av. Values 
               
             
          
           
               
                 Poloxamer No. 
                 Pluronic 
                 Av. Mol. Wt 
                 a 
                 b 
                 c 
               
               
                   
               
               
                 401 
                   
                 4,400 
                  6 
                 67 
                  6 
               
               
                 402 
                   
                 5,000 
                 13 
                 67 
                 13 
               
               
                 403 
                   
                 5,750 
                 21 
                 67 
                 21 
               
               
                 407 
                 F127 
                 12,000  
                 98 
                 67 
                 98 
               
               
                 331 
                   
                 3,800 
                  7 
                 54 
                  7 
               
               
                 333 
                   
                 4,950 
                 20 
                 54 
                 20 
               
               
                 334 
                   
                 5,850 
                 31 
                 54 
                 31 
               
               
                 335 
                   
                 6,000 
                 38 
                 54 
                 38 
               
               
                 338 
                 F108 
                 15,000  
                 128  
                 54 
                 128  
               
               
                 282 
                   
                 3,650 
                 10 
                 47 
                 10 
               
               
                 284 
                   
                 4,600 
                 21 
                 47 
                 21 
               
               
                 288 
                 F98 
                 13,500  
                 122  
                 47 
                 122  
               
               
                 231 
                   
                 2,750 
                  6 
                 39 
                  6 
               
               
                 234 
                   
                 4,200 
                 22 
                 39 
                 22 
               
               
                 235 
                   
                 4,600 
                 27 
                 39 
                 27 
               
               
                 237 
                 F87 
                 7,700 
                 62 
                 39 
                 62 
               
               
                 238 
                 F88 
                 10,800  
                 97 
                 39 
                 97 
               
               
                 212 
                   
                 2,750 
                  8 
                 35 
                  8 
               
               
                 215 
                   
                 4,150 
                 24 
                 35 
                 24 
               
               
                 217 
                 F77 
                 6,600 
                 52 
                 35 
                 52 
               
               
                 181 
                   
                 2,000 
                  3 
                 30 
                  3 
               
               
                 182 
                   
                 2,500 
                  8 
                 30 
                  8 
               
               
                 183 
                   
                 2,650 
                 10 
                 30 
                 10 
               
               
                 184 
                   
                 2,900 
                 13 
                 30 
                 13 
               
               
                 185 
                   
                 3,400 
                 19 
                 30 
                 19 
               
               
                 188 
                 F68 
                 8,350 
                 75 
                 30 
                 75 
               
               
                 122 
                   
                 1,630 
                  5 
                 21 
                  5 
               
               
                 123 
                   
                 1,850 
                  7 
                 21 
                  7 
               
               
                 124 
                   
                 2,200 
                 11 
                 21 
                 11 
               
               
                 101 
                   
                 1,100 
                  2 
                 16 
                  2 
               
               
                 105 
                   
                 1,900 
                 11 
                 16 
                 11 
               
               
                 108 
                 F38 
                 5,000 
                 46 
                 16 
                 46 
               
               
                   
               
             
          
         
       
     
     Certain number of these poloxamers are also known as Pluronic, which is a brand name of BASF Corporation. 
     Preferred poloxamers for use in the present invention are: 
     Pluronic F127 
     Pluronic F108 
     Pluronic F98 
     Pluronic F87 
     Pluronic F88 
     Pluronic F77 
     Pluronic F68 and 
     Pluronic F38. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The present invention is based on the discovery that particulate crystalline materials can be rendered mucoadhesive or bioadhesive, especially in the gastrointestinal tract, when the particles of poorly soluble crystalline therapeutic agents have certain poloxamers adsorbed on their surface. 
     The invention can be practiced with a wide variety of crystalline agents that are water-insoluble or poorly soluble in water. As used herein “poorly soluble” means that the agent has a solubility in aqueous medium of less than about 10 mg/ml, and preferably of less than about 1 mg/ml, must preferably less than 0.1 mg/ml. Examples of preferred crystalline agents follow. 
     Therapeutic Agents 
     Suitable therapeutic agents can be selected from a variety of known classes of drugs which are known to be absorbed across muscous membrances, including, for example, analgesics, anti-inflammatory agents, anthelmintics, anti-arrhythmic agents, antibiotics (including penicillins), anticoagulants, antidepressants, antidiabetic agents, antiepileptics, antifungal agents, antihistamines, antihypertensive agents, antimuscarinic agents, antimycobacterial agents, antineoplastic agents, immunosuppressants, antithyroid agents, antiviral agents, anxiolytic sedatives (hypnotics and neuroleptics), astringents, beta-adrenoceptor blocking agents, cardiac inotropic agents, contrast media, corticosteroids, cough suppressants (expectorants and mucolytics), diagnostic agents, diagnostic imaging agents, diuretics, dopaminergics (antiparkinsonian agents), haemostatics, immunological agents, lipid regulating agents, muscle relaxants, parasympathomimetics, parathyroid calcitonin and biphosphonates, protease inhibitors, prostaglandins, radio-pharmaceuticals, sex hormones (including steroids), anti-allergic agents, stimulants and anoretics, sympathomimetics, thyroid agents, vasodilators and xanthines. 
     Especially preferred are those drugs that are absorbed in the GI tract, such drugs, include, for example, antacids, anti-inflammatory agents, antibiotics (including penicillins), antimycobacterial agents, antiviral agents, corticosteroids, parasympathomimetics, radio-pharmaceuticals, sympathomimetics, demulcents, emollients, gastrointestinal protectives and adsorbents, antifungals, H2-blocking agents, proton pump inhibitors, muscarinic antagonists, bismuth compounds, sucralfate, carbenoxolone, prostaglandins, digestants, bile acids, laxatives, antiparasitic agents, anthelmintics, antiprotozoal agents, antimicrobial agents, vitamins, immunologic agents, vaccines, anesthetics, lipid-regulating agents and bile acid sequestrants. 
     A description of these classes of drugs and a listing of species within each class can be found in Martindale, The Extra Pharmacopoeia, Twenty-Ninth Edition, The Pharmaceutical Press, London, 1989, the disclosure of which is hereby incorporated by reference in its entirety. The drug substances are commercially available and/or can be prepared by techniques known in the art. 
     Preferred therapeutic agents include those intended for oral administration and rectal administration. 
     Method of Preparing the Particles of the Therapeutics Agents 
     As used herein, particle size is determined on the basis of weight average particle size as measured by conventional particle size measuring techniques well known to those skilled in the art, such as sedimentation field flow fractionation, photon correlation spectroscopy, or disk centrifugation. In present invention, the weight average particle size of the crystals of therapeutic agent and surface modifier is less than about 2,000 nm, preferred is less than about 1,000 nm, and more preferred is less than about 400 nm. It is particularly preferred that the particles of the present invention have an effective particle size which is less than 2,000 nm, the preferred 1,000 nm or the more preferred 400 nm. By “effective particle size” is meant that at least 90% of the particles in the sample measured by the above-noted weight sizing techniques have a particle size less than the stated size. With reference to the effective particle size, it is more preferred that at least 95% and, even more preferably, at least 99% of the particles have a particle size less than the desired particle size. 
     The particles of this invention can be prepared in a method comprising the steps of dispersing a therapeutic agent in a liquid dispersion medium and applying mechanical means in the presence of grinding media to reduce the particle size of the therapeutic agent to the desired effective average particle size. The particles are reduced in size in the presence of the poloxamer. Alternatively, the particles can be intimately mixed with a poloxamer after attrition. 
     A general procedure for preparing the particles of this invention is set forth below. The therapeutic agent is obtained commercially and/or prepared by techniques known in the art in a conventional coarse form. It is preferred, but not essential, that the particle size of the coarse substance selected be less than about 100 microns as determined by sieve analysis. If the coarse particle size of the substance is greater than about 100 microns, then it is preferred that the particles of the substance be reduced in size to less than 100 microns using a conventional milling method such as airjet or fragmentation milling. 
     The mechanical means applied to reduce the particle size of the substance conveniently can take the form of a dispersion mill. Suitable dispersion mills include a ball mill, an attritor mill, a vibratory mill, and media mills such as a sand mill and a bead mill. A media mill is preferred due to the relatively shorter milling time required to provide the intended result, i.e., the desired reduction in particle size. 
     The grinding media for the particle size reduction step can be selected from rigid media preferably spherical or particulate in form having an average size less than about 3 mm and, more preferably, less than about 1 mm. Such media desirably can provide the particles of the invention with shorter processing times and impart less wear to the milling equipment. The selection of material for the grinding media is not believed to be critical. We have found that zirconium oxide, such as 95% ZrO stabilized with magnesia, zirconium silicate and glass grinding media provide particles having levels of contamination which are believed to be acceptable for the preparation of pharmaceutical compositions. However, other media, such as stainless steel, titania, alumina, and 95% ZrO stabilized with yttrium, are expected to be useful. Preferred media have a density greater than about 3 g/cm 3 . 
     The attrition time can vary widely and depends primarily upon the particular mechanical means and processing conditions selected. For ball mills, processing times of up to five days or longer may be required. On the other hand, processing times of less than 1 day (residence times of one minute up to several hours) have provided the desired results using a high shear media mill. 
     The particles must be reduced in size at a temperature which does not significantly degrade the therapeutic agent. Processing temperatures of less than about 30°-40° C. are ordinarily preferred. If desired, the processing equipment can be cooled with conventional cooling equipment. The method is conveniently carried out under conditions of ambient temperature and at processing pressures which are safe and effective for the milling process. For example, ambient processing pressures are typical of ball mills, attritor mills and vibratory mills. Processing pressures up to about 20 psi (1.4 kg/cm 2 ) are typical of media milling. 
     The particle size was determined during the milling process and again immediately before the suspensions were administered. Particle size was determined on the Coulter Model N4MD Submicron Particle Analyzer (Coulter Corp.; Miami Lakes, Fla.); and using the Microtrac Ultrafine Particle Analyzer (Leeds and Northrup Co.; St. Petersburg, Fla.). 
     The following formulation examples will further illustrate the present invention. 
     
       
         
               
             
               
               
               
               
             
               
             
               
               
               
               
             
               
             
               
               
               
               
             
               
             
               
               
               
               
             
               
             
               
               
               
               
             
           
               
                   
               
             
             
               
                 Example 1 
               
             
          
           
               
                   
                 Paclitaxol 
                 10 
                 g 
               
               
                   
                 Pluronic F108 
                 5.0 
                 g 
               
               
                   
                 Benzoate Sodium 
                 0.2 
                 g 
               
               
                   
                 Saccharin Sodium 
                 0.1 
                 g 
               
               
                   
                 FD &amp; C Red No. 40 
                 0.03 
                 g 
               
               
                   
                 Water, qs 
                 100 
                 ml 
               
             
          
           
               
                 Example 2 
               
             
          
           
               
                   
                 Naproxen 
                 5.0 
                 g 
               
               
                   
                 Pluronic F68 
                 3.0 
                 g 
               
               
                   
                 Benzoate Sodium 
                 0.2 
                 g 
               
               
                   
                 Sorbate Potassium 
                 0.15 
                 g 
               
               
                   
                 Saccharin Sodium 
                 0.1 
                 g 
               
               
                   
                 FD &amp; C Red No. 40 
                 0.03 
                 g 
               
               
                   
                 Water, qs 
                 100 
                 ml 
               
             
          
           
               
                 Example 3 
               
             
          
           
               
                   
                 Griseofulvin 
                 5.0 
                 g 
               
               
                   
                 Pluronic F127 
                 2.5 
                 g 
               
               
                   
                 Benzoate Sodium 
                 0.2 
                 g 
               
               
                   
                 Saccharin Sodium 
                 0.1 
                 g 
               
               
                   
                 FD &amp; C Red No. 3 
                 0.03 
                 g 
               
               
                   
                 Water, qs 
                 100 
                 ml 
               
             
          
           
               
                 Example 4 
               
             
          
           
               
                   
                 Paclitaxol 
                 0.5 
                 g 
               
               
                   
                 Sucrose 
                 10 
                 g 
               
               
                   
                 Pluronic F87 
                 .25 
                 g 
               
               
                   
                 Dioctylsulfosuccinate 
                 0.1 
                 g 
               
               
                   
                 Methylparabens 
                 0.2 
                 g 
               
               
                   
                 Propylparabens 
                 0.07 
                 g 
               
               
                   
                 FD &amp; C Yellow No. 5 
                 0.03 
                 g 
               
               
                   
                 Water, qs 
                 100 
                 ml 
               
             
          
           
               
                 Example 5 
               
             
          
           
               
                   
                 Phenytoin 
                 5.0 
                 g 
               
               
                   
                 Pluronic F108 
                 2.5 
                 g 
               
               
                   
                 Sorbitol 
                 5 
                 g 
               
               
                   
                 Benzoate Sodium 
                 0.2 
                 g 
               
               
                   
                 Water, qs 
                 100 
                 ml 
               
               
                   
                   
               
             
          
         
       
     
     The following Table 2 is a listing of formulations comprised of particles of therapeutic agents produced by wet grinding in the presence of the poloxamer listed which resulted in the particles having the poloxamer absorbed on the surface thereof. The weight average particle size of the resulting particles is also provided 
     
       
         
               
               
               
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                   
                   
                   
                   
                 Particle Size 
               
               
                 Therapeutic 
                 Therapeutic 
                   
                 % 
                 Weight 
               
               
                 Agent 
                 Agent % 
                 Poloxamer 
                 Poloxamer 
                 Average 
               
               
                   
               
             
             
               
                 naproxen 
                 5% 
                 F-68 
                 3.0% 
                 270 nm 
               
               
                 indomethacin 
                 5% 
                 F-68 
                 1%   
                 228 nm 
               
               
                 indomethacin 
                 5% 
                 F-68 
                 2.5% 
                 193 nm 
               
               
                 ibuprofen 
                 3% 
                 F-68 
                 2.0% 
                 253 nm 
               
               
                 phenytoin 
                 5% 
                 F-68 
                 2.5% 
                 385 nm 
               
               
                 phenytoin 
                 5% 
                 F-108 
                 2.5% 
                 385 nm 
               
               
                 phenytoin 
                 5% 
                 F-127 
                 2.5% 
                 431 nm 
               
               
                 griseofulvin 
                 5% 
                 F-68 
                 2.5% 
                 617 nm 
               
               
                 griseofulvin 
                 5% 
                 F-127 
                 2.5% 
                 617 nm 
               
               
                 amphotericin B 
                 5% 
                 F-68 
                 2.5% 
                 426 nm 
               
               
                 amphotericin B 
                 5% 
                 F-127 
                 2.5% 
                 510 nm 
               
               
                 methotrexate 
                 5% 
                 F-68 
                 2.5% 
                 196 nm 
               
               
                 methotrexate 
                 5% 
                 F-127 
                 2.5% 
                 248 nm 
               
               
                 beclomethasone 
                 5% 
                 F-68 
                 2.5% 
                 221 nm 
               
               
                 dipropionate 
               
               
                 beclomethasone 
                 5% 
                 F-127 
                 2.5% 
                 244 nm 
               
               
                 dipropionate 
               
               
                 cyclosporine 
                 5% 
                 F-127 
                  4.75% 
                 262 nm 
               
               
                 cyclosporine 
                 5% 
                 F-127 
                 2.5% 
                 245 nm 
               
               
                 cyclosporine 
                 5% 
                 F-127 
                 1.0% 
                 240 nm 
               
               
                 cyclosporine 
                 5% 
                 F-127 
                 0.5% 
                 242 nm 
               
               
                 camptothecin 
                 2% 
                 F-68 
                 2%   
                 107 nm 
               
               
                 camptothecin 
                 2% 
                 F-108 
                 1%   
                 111 nm 
               
               
                 busulfan 
                 2% 
                 F-108 
                 0.5% 
                 387 nm 
               
               
                 piposulfan 
                 2% 
                 F-108 
                 0.5% 
                 339 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-68 
                  0.13% 
                 172 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-68 
                  0.25% 
                 247 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-87 
                  0.25% 
                 225 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-108 
                 0.2% 
                 195 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-108 
                  0.13% 
                 165 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-108 
                 0.5% 
                 190 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-108 
                 0.4% 
                 190 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-108 
                  0.25% 
                 206 nm 
               
               
                 paclitaxol 
                   0.5% 
                 F-108 
                 0.3% 
                 189 nm 
               
               
                 paclitaxol 
                 1% 
                 F-108 
                 0.5% 
                 143 nm 
               
               
                 paclitaxol 
                 2% 
                 F-108 
                 1%   
                 137 nm 
               
               
                 paclitaxol 
                 5% 
                 F-108 
                 5%   
                 197 nm 
               
               
                 paclitaxol 
                 5% 
                 F-108 
                  1.25% 
                 142 nm 
               
               
                 paclitaxol 
                 5% 
                 F-108 
                 2.5% 
                 173 nm 
               
               
                 paclitaxol 
                   2.3% 
                 F-108 
                  1.15% 
                 275 nm 
               
               
                 paclitaxol 
                 6% 
                 F-108 
                 3%   
                 175 nm 
               
               
                 paclitaxol 
                 10%  
                 F-108 
                 5%   
                 169 nm 
               
               
                   
               
             
          
         
       
     
     Determining Mucoadhesion Efficacy in the GI Tract 
     The amount and site of the mucoadhesion was determined by x-ray diagnostic imaging. The imaging was performed in anesthetized rats with the exception of G05-R1 samples which were imaged in fasted and anesthetized ferrets. Images were obtained using the Siemens C-Arm Siremobil 3U x-ray unit. The imaging dose was 10 ml/kg administered via gastric intubation to the anesthetized animal. X-rays were taken at 15, 30, 45 and 60 minutes and at 1, 2, 5 and 24 hours post-dose. A 10-15 ml volume of air was introduced to the animal at 30 minutes to produce a double contrast image. 
     Images were evaluated by the criteria of: coating, homogeneity, rate of gastric emptying and the total transit time. These are considered to be a measure of the stability of the suspension during transit down the GI tract and the ability of the formulation to adhere to the lower gastrointestinal tract. Suspensions were rated excellent when there was a uniform coating with transradiation of long intestinal segments, sufficient radiodensity to delineate anatomical structure, rapid emptying and transit, and stability and homogeneity during GI transit. A plus sign (+) was assigned when imaging in the lower GI was exceptional, a minus sign (−) as given when it was not. The suspension of the imaging agent [WIN 8883, ethyl 3,5-bis(acetylamino)-2,4,6-triodobenzoate], milled in Pluronic F127, was considered to be excellent for both upper and lower GI imaging in both rats and ferrets. All other suspensions were compared with this formulation. 
     The efficacy of the particulate formulations to image the GI tract is shown in Table 3. 
     
       
         
               
             
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Imaging Efficacy of Particulate Formulations Prepared 
               
               
                 with Pluronic F127 
               
             
          
           
               
                   
                   
                 Aqueous 
                   
                   
                 Particle 
                   
                 Imaging 
               
               
                   
                   
                 Solubility 
                 Days 
                 Milling 
                 Size 
                   
                 Efficacy 
               
               
                 Code 
                 WIN No. 
                 (μg/ml) 
                 Milled 
                 Process (a)   
                 (nm) (b)   
                 Range (c)   
                 
                   (d,e) 
                 
               
               
                   
               
               
                 GO5 
                 8883 
                 &lt;5 
                 1.8 
                 P 
                 186/ND 
                 Narrow 
                 ++++(*) 
               
               
                 GO5 R2 
                 8883 
                 &lt;5 
                 5   
                 JM 
                 135/ND 
                 80-230 
                 ++++(*) 
               
               
                   
               
               
                   (a) P = Planetary Mill, JM = Jar Mill  
               
               
                   (b) Post-milling/pre-dose particle size; ND = Not Determined.  
               
               
                   (c) Range(nm) = Size Distribution for 10 to 99% of the particles by weight.  
               
               
                   (d) Imaging efficacy is indicated as follows:  
               
               
                 Excellent ++++ 
               
               
                 Good +++ 
               
               
                 Fair ++ 
               
               
                 Poor + 
               
               
                   (e) Formulations with efficacy followed by (*) signs indicate exceptional lower GI imaging while those with (−) sign were found to be unacceptable in the lower GI.  
               
             
          
         
       
     
     Efficacy of Particle Formulations Prepared with Alternate Stabilizers 
     The imaging efficacy of suspension formulations with alternate stabilizers are shown in Table 4. DOSS is Diioctylsulfosuccinate and was used as a secondary stabilizer is some formulations. 
     Excellent imaging was obtained from suspensions of WIN 8883 stabilized with Pluronic F77 with 0.1% DOSS added (G29) and with Pluronic F88 (GO4), however, these suspensions did not image the lower GI as effectively as GO5. 
     Good imaging was obtained from suspensions of WIN 8883 stabilized with F88 with 0.1% DOSS (G27), and F87 (G26). Good upper GI imaging was obtained from suspensions of WIN 8883 stabilized with F98 (G14), F108 (G15) and F68 with 0.1% w/v DOSS (G23). Fair imaging was obtained from the WIN 8883 suspensions stabilized F87 with 1% w/v DOSS (G25). Poor imagining was obtained when no stabilizer was used (GO2) and the formulation with F77 alone (G28) gelled during milling and therefore was not imaged. 
     DOSS was needed to stabilize the suspension prepared in 4% w/v Pluronic F77 (G29). The same formulation without DOSS could not be imaged due to gelling during the milling process. 
     DOSS had variable effects when used in conjunction with other stabilizers. The suspension stabilized with F88 (GO4) was rated as excellent. When 0.1% w/v DOSS was used as a secondary stabilizer (G24), the imaging efficacy was rated only as good. A similar result was noted with suspensions stabilized with F87. The suspension without DOSS (G26) was rated higher in imaging efficacy than did the same suspension with 0.1% w/v DOSS (G25). Suspensions stabilized with 4% w/v tyloxapol plus 0.1% w/v DOSS (G27) or without DOSS (G20) were both rated good. Milling time, however, was reduced and overall particle size was smaller with the DOSS-added suspension. 
     
       
         
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Imaging Efficacy of Diagnostic Agents Prepared with 
               
               
                 Poloxamer and Alternative Stabilizers 
               
             
          
           
               
                   
                   
                   
                   
                 Particle 
                   
                 Imaging 
               
               
                   
                 Stabilizer 
                 Days 
                 Milling 
                 Size 
                   
                 Efficacy 
               
               
                 I.D. 
                 (w/v) 
                 Milled 
                 Process 
                 (nm) (b)   
                 Range (c)   
                 
                   (d,e,f) 
                 
               
               
                   
               
               
                 GO2 
                 None 
                 6 
                 JM 
                 1000/ND 
                 Broad 
                 + 
               
               
                 GO5 
                 4% F127 
                   1.8 
                 P 
                 186/ND 
                 Narrow 
                 ++++(*) 
               
               
                 G29 
                 4% F77/0.1% 
                 5 
                 JM 
                 146/187 
                 66-243 
                 ++++ 
               
               
                   
                 DOSS 
               
               
                 EGO4 
                 4% F88 
                   1.8 
                 P 
                 183/ND 
                 Narrow 
                 ++++ (−)   
               
               
                 G21 
                 4% PVA 
                 6 
                 JM 
                 204/199 
                 134-405 
                 +++(*) 
               
               
                 G26 
                 4% F87 
                 5 
                 JM 
                 155 
                  55-265 
                 +++ 
               
               
                 G20 
                 4% Tyloxapol 
                 6 
                 JM 
                 180/262 
                 137-521 
                 +++ 
               
               
                 G22 (f)   
                 2% HPMC 
                 6 
                 JM 
                 334/700 
                  350-2596 
                 +++ 
               
               
                 G24 
                 4% F88/0.1% 
                 5 
                 JM 
                 160 
                 146-265 
                 +++ 
               
               
                   
                 DOSS 
               
               
                 G27 
                 4% 
                 5 
                 JM 
                 140 
                  66-315 
                 +++ 
               
               
                   
                 Tyloxapol/ 
               
               
                   
                 0.1% DOSS 
               
               
                 G23 
                 5% F68/0.1% 
                 4 
                 JM 
                 147/170 
                 108-602 
                 +++ (−)   
               
               
                   
                 DOSS 
               
               
                 GO3 
                 4% Tween 80 
                 6 
                 JM 
                 161/ND 
                 Narrow 
                 ++ 
               
               
                 G18 (f)   
                 1% DOSS 
                 4 
                 JM 
                 119/130 
                  85-247 
                 ++ 
               
               
                 G19 (f)   
                 4% PVP 
                 5 
                 JM 
                 673/823 
                  620-1265 
                 ++ 
               
               
                 G25 
                 4% F87/0.1% 
                 5 
                 JM 
                 150 
                  66-243 
                 ++ 
               
               
                   
                 DOSS 
               
               
                   
               
               
                   (a) P = Planetary Mill, JM = Jar Mill, P(N)R = 18 hour planetary mill and N = number of days Jar Mill  
               
               
                   (b) Post-milling/pre-dose particle size; ND = Not Determined. Formulations G24 through G28, G30 and G32 were sized within 24 hours of milling; others were sized when the milling was terminated.  
               
               
                   (c) Range(nm) = Size Distribution for 10 to 99% of the particles by weight.  
               
               
                   (d) Imaging efficacy is indicated as follows:  
               
               
                 Excellent ++++ 
               
               
                 Good +++ 
               
               
                 Fair ++ 
               
               
                 P + 
               
               
                   (e) Formulations with efficacy followed by (*) signs indicate exceptional lower GI imaging while those with (−) signs were found to be unacceptable in the lower GI.  
               
               
                   (f)  Foaming was evident in G18 (1% DOSS), very thick foam was found in G19 (4% PVP) and in G22 (2% HPMC).  
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Comparison of Efficacy Between Pluronic F88 
               
               
                 and Pluronic F127 
               
             
          
           
               
                   
                   
                   
                   
                 Milling 
                 Particle 
                   
                 Imaging 
               
               
                   
                   
                   
                 Days 
                 Process 
                 Size 
                   
                 Efficacy 
               
               
                 I.D. 
                 WIN No 
                 Pluronic 
                 Milled 
                 
                   (a) 
                 
                 (nm) (b)   
                 Range (c)   
                 
                   (d,e) 
                 
               
               
                   
               
               
                 GO5 
                 8883 
                 F127 
                 1.8 
                 P 
                 186/ND 
                 Narrow 
                 ++++(*) 
               
               
                 GO4 
                 8883 
                 F88 
                 1.8 
                 P 
                 183/ND 
                 Narrow 
                 ++++(−) 
               
               
                   
               
               
                   (a) P = Planetary Mill, JM = Jar Mill, P(N)R = 18 hour planetary mill and N = number of days Jar Mill  
               
               
                   (b) Post-milling/pre-dose particle size; ND = Not Determined.  
               
               
                   (c) Range(nm) = Size Distribution for 10 to 99% of the particles by weight (pre-image where indicated)  
               
               
                   (d) Imaging efficacy is indicated as follows:  
               
               
                 Excellent ++++ 
               
               
                 Good +++ 
               
               
                 Fair ++ 
               
               
                 P + 
               
               
                   (e) Formulations with efficacy followed by (+) signs indicate exceptional lower GI imaging while those with (−) signs were found to be unacceptable in the lower GI.  
               
             
          
         
       
     
     Summarizing the above-described test results, many different stabilizers were examined using suspensions of WIN 8883, of these, Pluronic F127 was considered excellent for imaging both the upper and lower GI. A suspension stabilized with F88 was judged as excellent but for the upper GI only. 
     These studies indicate the site at which certain mucoadhesive stabilizers accrete, thus the formulator can use these studies to determine which stabilizer to utilize with a particular therapeutic agent in order to have the particles accrete at the desired site. 
     The invention being thus described, it will be obvious that the same may be varied in many ways. Such variations are not to be regarded as a departure from the spirit and scope of the invention, and all such modifications as would be obvious to one skilled in the art are intended to be included within the scope of the following claims.