Abstract:
A compound, pharmaceutical composition and method for the treatment of mammals wherein the active therapeutic agent is a compound having the structure: 
     
       
                 
         
             
             
         
       
       
         
           
             wherein:
           R 1  is an electronegative substituent,   R 2  is R 1  or alkyl,   R 3  is H or O-alkyl,   R 4  and R 5  are the same or different and are alkyl and   R 6  is H or OH.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims the benefit of provisional patent application Ser. No. 60/877,623, filed, Dec. 29, 2006; the entire contents and disclosure of which is incorporated herein by reference. 
    
    
     FIELD OF THE INVENTION 
     The present invention relates to novel therapeutic agents suitable for the treatment of mammals those afflicted with viral and retroviral, in particular, HIV infections. 
     BACKGROUND OF THE INVENTION 
     The entire disclosure and content of each reference and patent referred to herein is incorporated by reference. 
     Flavonoids (benzo-y-pyrones), as shown in Scheme 1 are a class of about 4,000 natural compounds present in all vascular plants. They appear to exhibit a wide range of biological activities including anti-oxidative [H. Bao et al, Food Chemistry, 86, 517 (2004); T. Miura et al, Food and Chemical Toxicology 41, 759 (2003)], anti-inflammatory [J. J. A. Hendricks et al, Journal of Experimental Medicine, 200,1667 (2004); H. Y. Lin et al, Journal of Cellular Physiology, 202. 579 (2005); Y. S. Chi et al, Biochem. Pharmacol, 62, 1185 (2001)], cancer suppressing [T. Zhang et al, Biorganic &amp; Medicinal Chemistry, 12, 6097 (2004); C. A. Rowe et al, Journal of Medicinal Food, 7,402 (2004). X. Zheng et al, Bioorganic and Medicinal Chemistry Letters, 13, 3423 (2003; X. Zheng et al, Bioorganic and Medicinal Chemisty Letters, 13, 881 (2003)] and antiviral (anti-HIV) [W. Wang et al, Antiviral Research, 64, 189 (2004); T. B. Ng, et al, Life Sei., 61, 933 (1997); A. J. Vlietnick et al, Plant Flavonoids in Biol. And Medicine II: Biochem. Cell. and Medicinal Properties, 283 (1988).]. The average human diet contains about 1 g of flavonoids per day, assimilated through fruits, vegetables, red wine, tea etc. [G. Di Carlo et al, Life Sciences, 65, 337 (1999)]. 
     
       
                 
         
             
             
         
      
     
     A family of flavonoids are known to be selectively toxic to multidrug resistant cancer cells. Several recent studies have linked the regulation of P-glycoprotein (Pgp) gene expression to the expression of the drug-metabolizing P450 genes, with the speculation that, in normal cells, P-glycoprotein may function in conjunction with the P450 enzymes in the detoxication of xenobiotics. Indeed, benzo[a]pyrene, an inducer and a substrate for cytochrome P4501a1, is also a substrate for P-glycoprotein. While investigating the coordinated regulation of these genes following exposure of multidrug resistant (MDR) cells to inducers of P450 gene expression, it was observed that one of these inducers, β-naphthoflavone (βNF), was considerably more toxic to multidrug resistant cells than to their drug-sensitive counterparts. This collateral sensitivity to βNF and other flavonoid compounds has now been further investigated in several multidrug resistant cell lines. For a further discussion of the anti-cancer properties of flavonoids, see US. H1,427; U.S. Pat. Nos. 5,336,685; 6,706,865 and 6,528,042. 
     Plant flavonoids usually occur in plants as glycosides, although in some circumstances they may occur as free aglycones. Most glycosides are O-glycosides, with the most common monoglycoside being at the 7-position. Diglycosides usually have sugars at the −7 and −3 positions and occasionally the −7 and −4′ positions. Other combinations and mono-glycosides exist but are less abundant. C-glycosides also occur in a more restricted distribution with C-6 and C-8 glycosides being the most common. 
     Plant flavonoids have antioxidative properties, cytostatic effects in tumorigenesis, and the ability to inhibit a broad spectrum of enzymes, such as angiotensin converting enzyme (ACE), protein kinase C, tyrosine protein kinase, and topoisomerase II. They are regarded as potential cancer preventatives and cardioprotective agents. Their potential use as anti-inflammatory or antiviral agents has also been examined. It is also disclosed in the literature that bioflavonoids, especially rutin, citrin, quercetin, hesperidin or derivatives are responsible for the inactivation of protein-cleaving enzymes (such as hyaluronidase and/or collagenase), which promote skin-aging processes. These compounds are used for general skin care or cosmetic surgery. It is reported that rutin, quercetin, isoquercitrin, catechin and other compounds also prevent and ameliorate the aging phenomena of the skin. It is also said that, together, quercetin glycoside, divalent metal ion, and extract of liquorice prevent intoxication by promoting alcohol metabolism in the human liver. 
     Rutin is a flavonoid glycoside comprised of quercetin and the sugar, rutinose. Many beneficial health effects of rutin have been demonstrated. Such effects have been attributed to anti-inflammatory, anti-mutagenic, anti-tumor, anticarcinogenic, smooth muscle relaxation, and estrogen receptor binding activities of rutin. Rutin is also being used in the treatment of capillary fragility, cerebral thrombosis, retinitis and rheumatic-fever-associated hemorrhagic conditions. Under conditions of low dietary fat intake, rutin and quercetin have been reported to considerably suppress colon tumor incidence. Backhaus (Use of bioflavonoid, especially rutin for retrovirus inactivation. Germany Patent DE4340438) claimed that rutin and its derivatives, in an oral dosage form, and injection or infusion solution, or a suppository, would inactivate retroviruses (e.g., HIV). 
     Most of the flavonoids have been isolated from plants in order to test their biological properties; others have been synthetically produced in search of more potent drug candidates. 
     The structure-activity relationship (SAR) in flavonoids is empirical, i.e., based on numerous data from testing different compounds. Although there are a lot of data in the literature, it is very difficult to outline a clear tendency that will lead to an optimized structure of high activity and low toxicity. 
     Among the most studied compounds, quercetin and baicalin are polyhydroxylated flavones [L. M. Larocca et al, J. Ural., 152, 1029 (1994) 1417; K. Ono et al, Biochem. Biophys. Res. Commun., 160, 982 (1989); B. Q. Li et al, Cell And Mol. Biol. Res., 39, 119 (1993)]. It is believed that the important structural features leading to high biological activities are the presence of 5-OH and 3-OH groups. The latter, however, is considered to be responsible for some mutagenic properties observed in quercetin, which disappeared after this group was methylated. Their solubilities can be modified through selective introduction of lipophilic and hydrophilic substituents. The most popular approach is to balance the number of the alkyl substituents and free hydroxyl groups. 
     The incorporation of electronegative elements, such as halogens and nitro groups in the flavonoid structure, introduce new patterns of biological properties. 4′,6-dichloroflavan has been reported to be a potent antirhinovirus compound. Halogenated and nitro-substituted flavones exhibit structure-dependent aryl hydrocarbon receptor (AhR) agonist and antagonist activities comparable to that observed for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) [Y. F. Lu et al, Biochemical Pharmacology. 51. 1077 (1996)]. 8-lodo, 8-bromo, and 8-trifluoromethyl derivatives of chrysin exhibit strong activities against human gastric adenocarcinoma cell lines (SGC-7901) and colorectal adenocarcinoma (HT-29) cells. One of the most recent studies reports the effect of some flavonoids on the central nervous system. 
     Halogenated flavanones and flavones are considered potential benzodiazepine receptor ligands. Indeed, 6-bromoflavone and 6-bromo-3′-nitroflavone showed activities close or higher than that of diazepam [P. Bovichelli et al, Tetrahedron Letters, 43, 5563 (2002)]. 
     It is an object of the present invention to provide novel chalcones and flavones substituted with electronegative groups useful for the treatment of mammals (man or animal), in particular, mammals afflicted with viral and retroviral, in particular, HIV infections and methods for their synthesis. 
     SUMMARY 
     The above and other objects are realized by the present invention, one embodiment of which relates to novel compounds having the structure: 
     
       
                 
         
             
             
         
      
     
     wherein: 
     R 1  is an electronegative substituent, 
     R 2  is R 1  or alkyl, 
     R 3  is H or O-alkyl, 
     R 4  and R 5  are the same or different and are alkyl, and 
     R 6  is H or OH. 
     A further embodiment of the invention concerns methods for the syntheses of the above described compounds comprising synthesizing the chalcones by the Claisen condensation of appropriately substituted acetophenones and benzaldehydes, preferably in ethanol and synthesizing the flavones by a three step Baker-Venkataraman rearrangement. 
     An additional embodiment are pharmaceutical compositions comprising therapeutically effective amounts for the treatment of viral and retroviral, in particular, HIV infections of a compound or mixture of compounds as described above and pharmaceutically acceptable carriers or excipients thereof. 
     A further embodiment of the invention comprises methods for the treatment of viral and retroviral, in particular, HIV infections in mammals in need thereof comprising administering thereto a therapeutically effective amount of a compound or mixture of compounds as described above. 
     Still further embodiments of the invention relate to (1) articles comprising packaging material which contains at least one biologically active agent contained within the packaging material, wherein the at least one therapeutic agent is effective for the treatment of a subject requiring treatment for viral and retroviral, in particular, HIV infections, and wherein the packaging material comprises a label which indicates that the therapeutic agent can be used for at least ameliorating the symptoms with which the subject is afflicted, and wherein the at least one biologically active agent is a compound or mixture of compounds as described above and (2) kits comprising, in separate packages: (A) a therapeutically effective amount of a compound or mixture of compounds as described above and (B) a carrier or excipient therefore or a biologically active agent different from the compound or mixture of compounds. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIGS. 1-4  are graphical depictions of the pharmacological properties of the compounds of the invention. 
         FIG. 1  is a graph illustrating drug inhibition on MCF-7 cells by CHBr 2M, CHCl 2M, CHNM 2M, and AZT. 
         FIG. 2  is a graph illustrating drug inhibition on H-9 cells by CHBr 2M, CHCl 2M, CHNM 2M, and AZT. 
         FIG. 3  is a graph illustrating drug inhibition on PC-3 cells by CHBr 2M, CHCl 2M, CHNM 2M, and AZT. 
         FIGS. 4A and 4B  illustrates the structures, compositions and codes for selected chalcones ( FIG. 4A ) and flavones ( FIG. 4B ). 
         FIG. 5  is a graph illustrating drug inhibition on MCF-7 cells by CHCl 3M, CHNM 3M, and AZT. 
         FIG. 6  is a graph illustrating drug inhibition on PC-3 cells by CM-091303, CM-090803, CM-110104, and AZT. 
         FIG. 7  is a graph illustrating drug inhibition on MCF-7 cells by CM-091303, CM-090803, CM-110104, and AZT. 
         FIG. 8  is a graph illustrating drug inhibition on MCF-7 cells by M/R-67, M/R-64-1, M/R-68-1, M/R-63-1, and AZT. 
         FIG. 9  is a graph illustrating drug inhibition on MCF-7 cells by AZT, M/R-67, and M/R-68-1. 
     
    
    
     DETAILED DESCRIPTION 
     The present invention is predicated on the discovery that chalcones and flavones substituted as shown above have unexpected and unobvious therapeutic activities, in particular, anti-viral and -retroviral, in particular, anti-HIV activities. 
     The chalcone derivatives (5-10 in scheme 3 below and Table 1) were synthesized by Claisen condensation of appropriately substituted acetophenones and benzaldehydes, preferably in ethanol in the presence of 50% aqueous KOH. The reaction times as well as the yields vary depending upon the corresponding reagents. The compounds containing the dimethoxybenzaldehyde unit were obtained with lower yields than the trimethoxy derivatives. The crude products were contaminated with some starting materials which could easily be removed by column chromatography on silica gel using CH 2 Cl 2 :hexane as an eluent (Scheme 
     
       
                 
         
             
             
         
      
     
     Flavones (17-22; Table 1) were synthesized using a three step Baker-Venkataraman rearrangement. The crude product was purified by recrystallization and Chromatotron chromatography using CH 2 Cl 2 :MeOH as an eluent (Scheme 4). 
     
       
                 
         
             
             
         
      
     
     The structures of the synthesized compounds were confirmed by  1 H-NMR, IR and elemental analysis (table 2). The compounds were subjected to tests that confirmed their cyclooxygenase-1 and cyclooxygenase-2 binding and anti-HIV activities. 
     In the structural formulas I and II above, the alkyl groups may be straight or branch-chain and may contain from 1 to 6 carbon atoms. Preferred alkyl groups for R 2 , R 3 , R 4  and R 5  are methyl, ethyl, propyl, isopropyl and tertiary butyl; most preferred being methyl. 
     The electronegative substituents on the molecules depicted in formulas I and II may be halogen, preferably chloro and bromo, and nitro. 
     As used herein, “treating” describes the management and care of a patient (human or animal) for the purpose of combating a disease, condition, or disorder and includes the administration of a compound of the present invention to prevent the onset of the symptoms or complications, alleviating the symptoms or complications, or eliminating the disease, condition or disorder. 
     The dosage of compound employed will depend in each case upon the nature of the cancer being treated; on absorption, inactivation and excretion rates of the drug, as well as other factors known to those of skill in the art. It is to be noted that dosage values will also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens and schedules should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions. Generally, it is preferred to utilize orally administered compositions such as tablets or capsules containing 50-100 mg, administered twice a day for an adult patient weighing 70 kg (154 lbs). It will be understood by those skilled in the art, however, that any suitable mode of administration and appropriate dosage may be employed in the practice of the invention. 
     Although the methods and compositions of the invention may be employed to treat any viral and retroviral infection sensitive to the compounds of the invention, the invention is particularly suited for the treatment of HIV infections. 
     In the examples below, melting points were determined on a Gallenkamp Melting Point Apparatus and were uncorrected. Infrared spectra were obtained on a Perkin Elmer FTIR 1430 spectrophotometer, using KBr pellets.  1 H-NMR and  13 C-NMR spectra were obtained with a Brucker HX-300 spectrometer and the chemical shifts were reported as parts per million (5 ppm) downfield. 
     Column chromatography as well as Chromatotron 8924 (Harrison Research) instruments were used for purification of the compounds. Davisil Chromatographic Silica gel (200-425 mesh) was used for column chromatographic separations and Silica gel Merck, TLC grade 7749 with gypsum binder and fluorescent indicator was used for the preparation of the Chromatotron rotors. All reactions and purification procedures were monitored using Whatman TLC plates with a fluorescent indicator. 
     As noted above and shown in Scheme 5, chalcone derivatives are synthesized by the Claisen condensation of appropriately substituted and commercially available acetophenones and benzaldehydes in ethanol in the presence of 50% aqueous KOH. Corresponding substituted acetophenones were mixed with an equimolar amount of substituted benzaldehydes in ethanol. 5 milliliters of 50% aqueous KOH was added and the reaction mixture heated at 50-60° C. for 2-6 h. The product precipitated after cooling as yellow crystals, which were collected, recrystallized from CH 3 OH/CH 2 Cl and finally purified by column chromatography, using CH 2 Cl 2 :hexane 5:1 as eluent. 
     Synthesis of substituted chalcones: the methylated chalcone was synthesized by selective dealkylation of commercially available 2,4,6-trihydroxybenzaldehyde in the presence of aluminum tribromide using acetonitrile as the solvent. The aldehyde was reduced by means of triethylsilane in trifluoroacetic acid. Acylation of the product with acetic anhydride gave the appropriately substituted precursor for chalcone formation. 
     
       
                 
         
             
             
         
      
       
                 
         
             
             
         
       
     
     EXAMPLE 1 
     Synthesis of Chalcones 5-10 
     Employing the general method described by N. N. Mateeva et al, J. Heterocyclic Chem., 39, 1251 (2002); D. K. Bhardwaj et al, Indian J. Chem., 27B, 261 (1988) and K. Krohn et al, Phytochemistry 6 1.93 1 (2002), the corresponding substituted acetophenones 1 were mixed with an equimolar amount of 3,4-dimethoxybenzaldehyde or 3,4,5-trimethoxybenzaldehyde, 2, in ethanol (Scheme 3). 5 Milliliters of 50% aqueous KOH was added and the reaction mixture heated at 50-60° C. for 2-6 h. The product precipitated after cooling as yellow crystals, which were collected, recrystallized from CH 3 OH/CH 2 Cl 2  and finally purified by column chromatography, using CH 2 Cl 2 :hexane 5:1 as eluent. The yields were 60-80%. 
     EXAMPLE 2 
     Synthesis of Flavones 17-22 
     Employing the general method described by Mateeva, supra, T. Hone et al, Chem. Pharm. Bull., 43,2054 (1995) and C. J. Bennett et al, Bioorganic &amp; Medicinal Chemistry, 12,2079 (2004). Compounds 3 (Scheme 4) were mixed with 1.7 times excess of 3,4,5-trimethoxy benzoyl chloride or 3,4-dimethoxy benzoyl chloride, 4, correspondingly, in pyridine. The reaction mixture was heated at 70° C. for 4 h, and then poured onto ice containing 5 N HCL. The precipitate that formed was collected by vacuum filtration and air dried. The crude product was dissolved in pyridine in the presence of 10 times excess of KOH. 
     The reaction was carried out for 4 h at 65° C., and then the mixture poured onto an iced 5N HCl bath. A yellow precipitate was formed, which was collected by filtration and air dried. The crude product was dissolved in acetic acid containing 1 mL concentrated H 2 SO 4 , heated at 60° C. for 4 h and left stirring overnight at room temperature. After 24 h, the reaction mixture was poured onto an ice-NaHCO 3  mixture, the product collected by filtration and recrystallized from ethanol. 
     The pure flavone was obtained by purification on Chromatotrone with CH 2 Cl 2 :MeOH 8:1 as eluent. The yields were 30-50%. 
     The purified and identified analogs were subjected to in vitro biological testing. The results of the testing clearly demonstrated inhibition of the H-9 cell lines (the HIV or cancer virus cell lines), as well as significant inhibition of the MCF-7 cell lines (breast cancer cells) and PC-3 cell lines (prostate cancer cell lines). See tables 3-17. The biological activities of the flavonoids were compared with the activity of AZT (a well established anti-HIV and anti-cancer drug) under the same conditions. Three compounds (CHBr2M, CHCl2M and CHNM3M) showed a much superior inhibitory activities, compared to AZT when assayed against the above mentioned cell lines under the same conditions. 
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Elemental Analysis and Melting Point Data for the Synthesized Compounds 
               
             
          
           
               
                   
                   
                   
                 Elemental Analysis 
                 m.p. 
               
               
                   
                 Compound 
                 Composition 
                 Calculated/Found 
                 ° C. 
               
               
                   
                   
               
             
          
           
               
                 5 
                 R 1 ═R 2 ═Cl, R 3 ═OCH 3   
                 C 18 H 16 Cl 2 O 5   
                 C56.41 H4.21 
                 145-146 
               
               
                   
                   
                   
                 C56.31 H4.41 
               
               
                 6 
                 R 1 ═R 2 ═Br, R 3 ═OCH 3   
                 C 18 H 16 Br 2 O 5   
                 C45.79 H3.42 
                 152-153 
               
               
                   
                   
                   
                 C56.41 H4.21 
               
               
                 7 
                 R 1 ═NO 2 , R 2 ═CH 3 , R 3 ═OCH 3   
                 C 19 H 19 NO 7   
                 C61.12 H5.13 
                 147-148 
               
               
                   
                   
                   
                 C61.11 H5.40 
               
               
                 8 
                 R 1 ═R 2 ═Cl, R 3 ═H 
                 C 17 H 14 Cl 2 O 4   
                 C57.81 H4.00 
                 163-164 
               
               
                   
                   
                   
                 C58.03 H4.39 
               
               
                 9 
                 R 1 ═R 2 ═Br, R 3 ═H 
                 C 17 H 14 Br 2 O 4   
                 C46.18 H3.19 
                 158-159 
               
               
                   
                   
                   
                 C46.29 H3.43 
               
               
                 10 
                 R 1 ═NO 2 , R 2 ═CH 3 , R 3 ═H 
                 C 18 H 17 NO 6   
                 C62.97 H4.99 
                 153-154 
               
               
                   
                   
                   
                 C62.97 H5.33 
               
               
                 11 
                 R 1 ═R 2 ═Cl, R 3 ═OCH 3   
                 C 18 H 14 Cl 2 O 6   
                 C54.43 H3.55 
                 198-199 
               
               
                   
                   
                   
                 C54.36 H3.85 
               
               
                 12 
                 R 1 ═R 2 ═Br, R 3 ═OCH 3   
                 C 18 H 16 Br 2 O 6   
                 C44.47 H2.90 
                 &gt;260, 
               
               
                   
                   
                   
                 C44.12 H3.19 
                 decomp 
               
               
                 13 
                 R 1 ═NO 2 , R 2 ═CH 3 , R 3 ═OCH 3   
                 C 19 H 17 NO 8   
                 C58.92 H4.42 
                 210-211 
               
               
                   
                   
                   
                 C58.97 H4.71 
               
               
                 14 
                 R 1 ═R 2 ═Cl, R 3 ═H 
                 C 17 H 12 Cl 2 O 5   
                 C55.61 H3.29 
                 215-216 
               
               
                   
                   
                   
                 C55.41 H3.45 
               
               
                 15 
                 R 1 ═R 2 ═Br, R 3 ═H 
                 C 17 H 12 Br 2 O 5   
                 C44.77 H2.65 
                 220-221 
               
               
                   
                   
                   
                 C44.81 H2.99 
               
               
                 16 
                 R 1 ═NO 2 , R 2 ═CH 3 , R 3 ═H 
                 C 18 H 15 NO 7   
                 C60.50 H4.23 
                 195-196 
               
               
                   
                   
                   
                 C60.41 H4.21 
               
               
                 17 
                 R 1 ═R 2 ═Cl, R 3 ═OCH 3   
                 C 18 H 14 Cl 2 O 5   
                 C56.71 H3.70 
                 155-156 
               
               
                   
                   
                   
                 C57.03 H3.81 
               
               
                 18 
                 R 1 ═R 2 ═Br, R 3 ═OCH 3   
                 C 18 H 14 Br 2 O 5   
                 C45.99 H3.00 
                 179-180 
               
               
                   
                   
                   
                 C46.28 H3.39 
               
               
                 19 
                 R 1 ═NO 2 , R 2 ═CH 3 , R 3 ═OCH 3   
                 C 19 H 17 NO 7   
                 C61.45 H4.61 
                 135-136 
               
               
                   
                   
                   
                 C561.47 H5.01 
               
               
                 20 
                 R 1 ═R 2 ═Cl, R 3 ═H 
                 C 17 H 12 Cl 2 O 4   
                 C58.14 H3.44 
                 145-146 
               
               
                   
                   
                   
                 C58.37 H3.76 
               
               
                 21 
                 R 1 ═R 2 ═Br, R 3 ═H 
                 C 17 H 12 Br 2 O 4   
                 C46.40 H2.75 
                 134-135 
               
               
                   
                   
                   
                 C46.72 H3.12 
               
               
                 22 
                 R 1 ═NO 2 , R 2 ═CH 3 , R 3 ═H 
                 C 18 H 15 NO 6   
                 C63.34 H4.43 
                 150-151 
               
               
                   
                   
                   
                 C62.95 H4.80 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
             
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 NMR and IR Data for the Synthesized Compounds 
               
             
          
           
               
                 Compound 
                 1H-NMR (δ ppm) 
                 IR (ν cm −1  KBr) 
               
               
                   
               
             
          
           
               
                 5 
                 (DMSO-d 6 ): δ 3.70, 3.79 (s, s, 9H, —CH 3 O), 
                 3400 (broad, OH) 
               
               
                   
                 6.82 (s, 2H, Ar), 7.07-7.08 (d, 1H, Ar, J = 3.0 
                 1590 (C═O), 
               
               
                   
                 Hz), 7.42-7.43 (d, 1H, Ar, J = 3.0 Hz), 
                 1610 (C═C Ar), 
               
               
                   
                 7.37-7.42 (d, 1H, CH═CH, J = 15 Hz) 
                 1590 (C═C), 
               
               
                   
                 8.55-8.60 (d, 1H, CH═CH, J = 15 Hz) 
                 1130 (C—O) cm −1   
               
               
                 6 
                 (DMSO-d 6 ): δ 3.78, 3.80 (s, s, 9H, —CH 3 O), 
                 3390 (broad, OH) 
               
               
                   
                 7.14 (s, 2H, Ar), 7.34-7.35 (d, 1H, Ar, J = 3.0 Hz), 
                 1585 (C═O), 
               
               
                   
                 7.39-7.45 (d, 1H, Ar, J = 18 Hz, CH═CH, J = 15 Hz) 
                 1600 (C═C Ar), 
               
               
                   
                 7.58-7.59 (d, 1H, Ar, J = 3.0 Hz), 8.48-8.53 
                 1595 (C═C), 
               
               
                   
                 (d, 1H, J = 18 Hz) 
                 1110 (C—O) cm −1   
               
               
                 7 
                 (DMSO-d 6 ): δ 3.81, 3.84 (s, s, 9H, —CH 3 O), 
                 3390 (broad, OH) 
               
               
                   
                 6.87 (s, 2H, Ar), 7.37-7.43 (d, 1H, Ar, J = 18 Hz 
                 1640 (C═O), 
               
               
                   
                 CH═CH), 7.48-7.49 (d, 1H, J = 3.0 Hz, Ar), 
                 1612 (C═C Ar), 
               
               
                   
                 8.08-8.14 (d, 1H, J = 18 Hz, CH═CH) 
                 1580 (C═C), 
               
               
                   
                   
                 1120 (C—O) cm −1   
               
               
                 8 
                 (DMSO-d 6 ): δ 3.93, 3.97 (s, s, 6H, —CH 3 O), 
                 3400 (broad, OH) 
               
               
                   
                 6.88-6.92 (m, 1H, Ar), 7.15-7.16 (d, 1H, J = 
                 1650 (C═O), 
               
               
                   
                 3.0 Hz, Ar), 7.24-7.29 (m, 1H, Ar), 7.34-7.39 (d, 
                 1610 (C═C Ar), 
               
               
                   
                 1H, J = 15 Hz, CH═CH), 7.54-7.57 (m, 1H, Ar), 
                 1590 (C═C), 
               
               
                   
                 7.80-7.81 (d, 1H, J = 3.0 Hz, Ar), 7.91-7.96 (d, 1H, 
                 1125 (C—O) cm −1   
               
               
                   
                 J = 15 Hz, CH═CH) 
               
               
                 9 
                 (DMSO-d 6 ): δ 3.07, 3.10 (s, s, 6H, —CH 3 O), 
                 3390 (broad, OH) 
               
               
                   
                 6.88-6.92 (m, 1H, Ar), 7.15-7.16 (d, 1H, Ar), 
                 1650 (C═O), 
               
               
                   
                 7.26-7.30 (dd, 1H, Ar), 7.34-7.39 (d, 1H, J = 15 
                 1611 (C═C Ar), 
               
               
                   
                 Hz, CH═CH), 7.86-7.91 (d, 1H, J = 15 Hz, 
                 1590 (C═C), 
               
               
                   
                 CH═CH), 7.94-7.98 (m, 1H, Ar) 
                 1110 (C—O) cm −1   
               
               
                 10 
                 (DMSO-d 6 ): δ 3.77, 3.80 (s, s, 6H, —CH 3 O), 
                 3390 (broad, OH) 
               
               
                   
                 6.95-6.98 (d, 1H, J = 9 H), 7.17 (s, 1H, Ar), 
                 1640 (C═O), 
               
               
                   
                 7.22-7.23 (d, 1H, J = 3.0 Hz, Ar), 7.40-7.45 (d, 1H, 
                 1610 (C═C Ar), 
               
               
                   
                 J = 15 Hz, CH═CH), 7.65-7.66 (d, 1H, J = 3.0 Hz, 
                 1592 (C═C), 
               
               
                   
                 Ar), 798-8.03 (d, 1H, J = 15 Hz, CH═CH) 
                 1120 (C—O) cm −1   
               
               
                 11 
                 (DMSO-d 6 ): δ 3.68, 3.81 (s, s, 9H, —CH 3 O), 
                 3400 (broad, OH) 
               
               
                   
                 7.83-7.84 (d, 1H, Ar, J = 2.4 Hz), 7.85-7.86 (d, 1H, 
                 1650 (C═O), 
               
               
                   
                 Ar, J = 2.4 Hz), 8.08 (s, 2H, Ar) 
                 1613 (C═C Ar), 
               
               
                   
                   
                 1590 (C═C), 
               
               
                   
                   
                 1131 (C—O) cm −1   
               
               
                 12 
                 (DMSO-d 6 ): δ 3.68, 3.81 (s, s, 9H, —CH 3 O), 
                 3390 (broad, OH) 
               
               
                   
                 7.98-7.99 (d, 1H, Ar, J = 2.4 Hz), 8.01-8.02 (d, 1H, 
                 1655 (C═O), 
               
               
                   
                 Ar, J = 2.4 Hz), 8.19 (s, 2H, Ar) 
                 1605 (C═C Ar), 
               
               
                   
                   
                 1580 (C═C), 
               
               
                   
                   
                 1115 (C—O) cm −1   
               
               
                 13 
                 (DMSO-d 6 ): δ 3.76, 3.86 (s, s, 6H, —CH 3 O), 
                 3410 (broad, OH) 
               
               
                   
                 7.69 (s, 2H, Ar), 8.25-8.26 (d, 1H, J = 3.0 Hz, Ar), 
                 1660 (C═O), 
               
               
                   
                 8.42-8.43 (d, 1H, J = 3.0 Hz, Ar). 
                 1613 (C═C Ar), 
               
               
                   
                   
                 1595 (C═C), 
               
               
                   
                   
                 1115 (C—O) cm −1   
               
               
                 14 
                 (DMSO-d 6 ): δ 3.79 (s, s, 9H, —CH 3 O), 7.01-7.04 
                 3380 (broad, OH) 
               
               
                   
                 (d, 1H, Ar, J = 9.0 Hz), 7.81 (s, 1H, Ar), 
                 1645 (C═O), 
               
               
                   
                 7.87 (m, 1H, Ar), 8.12-8.15 (d, 1H, Ar, J = 9.0 Hz), 
                 1610 (C═C Ar), 
               
               
                   
                 8.44 (s, 1H, Ar) 
                 1595 (C═C), 
               
               
                   
                   
                 1120 (C—O) cm −1   
               
               
                 15 
                 (DMSO-d 6 ): δ 3.29, 3.31 (s, s, 6H, —CH 3 O), 
                 3400 (broad, OH) 
               
               
                   
                 7.17-7.19 (d, 1H, Ar, J = 6 Hz), 7.88-7.98 
                 1656 (C═O), 
               
               
                   
                 (m, 2H, Ar,), 8.14-8.15 (d, 1H, J = 3.0 Hz), 
                 1600 (C═C Ar), 
               
               
                   
                 8.34-8.35 (d, 1H, Ar, J = 3.0 Hz) 
                 1595 (C═C), 
               
               
                   
                   
                 1130 (C—O) cm −1   
               
               
                 16 
                 (DMSO-d 6 ): δ 3.77, 3.80 (s, s, 6H, —CH 3 O), 
                 3380 (broad, OH) 
               
               
                   
                 6.97-6.99 (m, 2H, Ar), 8.16 (s, 1H, Ar), 
                 1660 (C═O), 
               
               
                   
                 8.33-8.35 (dd, J = 6 Hz, Ar), 8.48 (s, 1H, Ar) 
                 1610 (C═C Ar), 
               
               
                   
                   
                 1588 (C═C), 
               
               
                   
                   
                 1130 (C—O) cm −1   
               
               
                 17 
                 (deuteriochloroform): δ 3.87, 3.89 (s, 9H, —CH 3 O), 
                 1660 (C═O), 
               
               
                   
                 8.01-8.02 (d, J = 2.4 Hz, 1H, Ar), 7.67-7.68 
                 1613 (C═C Ar), 
               
               
                   
                 (d, J = 2.4 Hz, 1H, Ar), 6.74 (s, 1H, O═C—CH═), 
                 1592 (C═C), 
               
               
                   
                 7.14 (s, 2H, Ar) 
                 1133 (C—O) cm −1   
               
               
                 18 
                 (deuteriochloroform): δ 3.93, 3.94 (s, 9H, —CH 3 O), 
                 1656 (C═O), 
               
               
                   
                 8.27-8.28 (d, J = 2.2 Hz, 1H, Ar), 8.02-8.03 
                 1610 (C═C Ar), 
               
               
                   
                 (d, J = 2.2 Hz, 1H, Ar), 6.80 (s, 1H, O═C—CH═), 
                 1591 (C═C), 
               
               
                   
                 7.23 (s, 2H, Ar) 
                 1134 (C—O) cm −1   
               
               
                 19 
                 (deuteriochloroform): δ 3.87, 3.91 (s, 9H, —CH 3 O), 
                 1654 (C═O), 
               
               
                   
                 8.24 (s, 1H, Ar), 8.19-8.20 (d, J = 2.1 Hz, 
                 1618 (C═C Ar), 
               
               
                   
                 1H, Ar), 6.79 (s, 1H, O═C—CH═), 7.23 (s, 2H, Ar), 
                 1583 (C═C), 
               
               
                   
                 2.49 (s, 3H, —CH 3 ) 
                 1126 (C—O) cm −1   
               
               
                 20 
                 (deuteriochloroform): δ 3.95, 3.96 (s, 6H, —CH 3 O), 
                 1654 (C═O), 
               
               
                   
                 8.07-8.06 (d, J = 2.3 Hz, 1H, Ar), 7.70-7.71 
                 1561 (C═C Ar), 
               
               
                   
                 (d, J = 2.6 Hz, 1H, Ar), 6.77 (s, 1H, O═C—CH═), 
                 1519 (C═C), 
               
               
                   
                 6.67-6.99 (d, J = 8.4 Hz, 1H, Ar) 7.44-7.45 
                 1278(C—O) cm −1   
               
               
                   
                 (d, J = 2.0 Hz, 1H, Ar), (td, J = 2.3, 8.7 Hz, 1H, Ar) 
               
               
                 21 
                 (deuteriochloroform): δ 3.92, 3.93 (s, 6H, —CH 3 O), 
                 1677 (C═O), 
               
               
                   
                 8.26-8.27 (d, J = 2.4 Hz, 1H, Ar), 7.99-8.0 
                 1650 (C═C Ar), 
               
               
                   
                 (d, J = 2.2 Hz, 1H, Ar), 6.82 (s, 1H, O═C—CH═), 
                 1599 (C═C), 
               
               
                   
                 6.67-6.99 (d, J = 8.4 Hz, 1H, Ar), 
                 1235 (C—O) cm −1   
               
               
                   
                 7.74-7.77 (dd, J = 1.9 Hz, 6.4 Hz 1H, Ar), 
               
               
                   
                 7.57-7.58 (d, J = 1.6 Hz, 6.4 Hz 1H, Ar), 
               
               
                   
                 6.88-6.91 (d, J = 8.5 Hz 1H, Ar) 
               
               
                 22 
                 (deuteriochloroform): δ 3.96, 4.00 (s, 6H, —CH 3 O), 
                 1647 (C═O), 
               
               
                   
                 8.30 (s, 1H, Ar), 8.22-8.23 (d, J = 2.0 Hz, 
                 1615 (C═C Ar), 
               
               
                   
                 1H, Ar), 6.84 (s, 1H, O═C—CH═), 6.97-7.00 (d, 
                 1531 (C═C), 
               
               
                   
                 J = 8.9 Hz, 1H, Ar), 7.82-8.00 (dd, J = 1.8 Hz, 50.3, 
                 1256 (C—O) cm −1   
               
               
                   
                 1H, Ar), 7.58-7.59 (d, J = 2.0 Hz, 1H, Ar), 2.54 (s, 
               
               
                   
                 3H, —CH 3 ) 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 K. K. Redda/ 
                   
                   
               
               
                 Chavor 
                   
                   
               
               
                 Exp.: 
                   
                 Dec. 05 to 
               
               
                 H-9 Cells 
                 Cytoxicity on H-9 cells 
                 Dec. 09, 2005 
               
             
          
           
               
                 Drugs 
                 Cells (×10 4 ) 
                 Mean 
                 SD 
                 % Inhibition 
                 Mean 
                 SD 
               
               
                   
               
             
          
           
               
                   
                 49.5 
                   
                   
                   
                   
                   
               
               
                 2% DMSO 
                 50.5 
                 50.25 
                 0.54 
                 0 
                   
                   
               
               
                   
                 50.8 
                   
                   
                   
                   
                   
               
               
                 AZT 
                 41.3 
                   
                   
                 17.91 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 37.5 
                 40.83 
                 2.57 
                 25.37 
                 18.74 
                 5.11 
               
               
                   
                 43.8 
                   
                   
                 12.94 
                   
                   
               
               
                   
                 30.5 
                   
                   
                 39.30 
                   
                   
               
               
                 5 
                 30.0 
                 30.92 
                 0.96 
                 40.30 
                 38.47 
                 1.92 
               
               
                   
                 32.3 
                   
                   
                 35.82 
                   
                   
               
               
                   
                 26.0 
                   
                   
                 48.26 
                   
                   
               
               
                 10  
                 25.0 
                 25.25 
                 0.54 
                 50.25 
                 49.75 
                 1.07 
               
               
                   
                 24.8 
                   
                   
                 50.75 
                   
                   
               
               
                 CHBr 2M 
                 45.5 
                   
                   
                 9.45 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 49.5 
                 47.42 
                 1.64 
                 1.49 
                 5.64 
                 3.26 
               
               
                   
                 47.3 
                   
                   
                 5.97 
                   
                   
               
               
                   
                 25.5 
                   
                   
                 49.25 
                   
                   
               
               
                 5 
                 24.8 
                 25.33 
                 0.42 
                 50.76 
                 49.59 
                 0.85 
               
               
                   
                 25.8 
                   
                   
                 48.76 
                   
                   
               
               
                   
                 18.3 
                   
                   
                 63.68 
                   
                   
               
               
                 10  
                 16.3 
                 17.58 
                 0.94 
                 67.66 
                 65.01 
                 1.88 
               
               
                   
                 18.3 
                   
                   
                 63.88 
                   
                   
               
               
                   
                 11.8 
                   
                   
                 76.62 
                   
                   
               
               
                 50  
                 11.5 
                 11.58 
                 0.12 
                 77.11 
                 76.95 
                 0.23 
               
               
                   
                 11.5 
                   
                   
                 77.11 
                   
                   
               
               
                 CHCl 2M 
                 42.0 
                   
                   
                 16.42 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 42.3 
                 42.25 
                 0.20 
                 15.92 
                 15.92 
                 0.41 
               
               
                   
                 42.6 
                   
                   
                 15.42 
                   
                   
               
               
                   
                 24.0 
                   
                   
                 52.24 
                   
                   
               
               
                 5 
                 23.8 
                 24.00 
                 0.20 
                 52.74 
                 52.24 
                 0.41 
               
               
                   
                 24.3 
                   
                   
                 51.74 
                   
                   
               
               
                   
                 13.0 
                   
                   
                 74.13 
                   
                   
               
               
                 10  
                 11.5 
                 12.92 
                 1.12 
                 77.11 
                 74.30 
                 2.24 
               
               
                   
                 14.3 
                   
                   
                 71.64 
                   
                   
               
               
                   
                 4.0 
                   
                   
                 92.04 
                   
                   
               
               
                 50  
                 4.5 
                 4.50 
                 0.41 
                 91.04 
                 91.04 
                 0.81 
               
               
                   
                 5.0 
                   
                   
                 90.05 
                   
                   
               
               
                 CHNM 2M 
                 57.3 
                   
                   
                 −13.93 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 60.3 
                 58.17 
                 1.48 
                 −19.90 
                 −15.75 
                 2.94 
               
               
                   
                 57.0 
                   
                   
                 −13.43 
                   
                   
               
               
                   
                 61.5 
                   
                   
                 −22.39 
                   
                   
               
               
                 5 
                 55.5 
                 58.58 
                 2.45 
                 −10.45 
                 −16.58 
                 4.88 
               
               
                   
                 58.8 
                   
                   
                 −16.92 
                   
                   
               
               
                   
                 50.5 
                   
                   
                 −0.50 
                   
                   
               
               
                 10  
                 52.3 
                 50.75 
                 1.14 
                 −3.98 
                 −1.00 
                 2.26 
               
               
                   
                 49.5 
                   
                   
                 1.49 
                   
                   
               
               
                   
                 56.8 
                   
                   
                 −12.94 
                   
                   
               
               
                 50  
                 54.8 
                 56.00 
                 0.89 
                 −8.96 
                 −11.44 
                 1.77 
               
               
                   
                 56.5 
                   
                   
                 −12.44 
                   
                   
               
               
                 H-9 
                 Mean 
                   
                   
                   
                   
                   
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
                 TABLE 4 
               
               
                   
                   
               
               
                   
                 1 
                 5 
                 10 
                 50 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Drugs (μg/ml) 
                   
                   
                   
                   
               
               
                   
                 CHBr 2M (μg/ml) 
                 5.64 
                 49.59 
                 65.01 
                 76.95 
               
               
                   
                 CHCl 2M (μg/ml) 
                 15.92 
                 52.24 
                 74.3 
                 91.04 
               
               
                   
                 CHNM 2M (μg/ml) 
                 −15.75 
                 −16.58 
                 −1 
                 −11.44 
               
               
                   
                 AZT (μg/ml) 
                 18.74 
                 38.47 
                 49.75 
               
               
                   
                 SD 
               
               
                   
                 Drugs 
               
               
                   
                 CHBr 2M (μg/ml) 
                 3.26 
                 0.85 
                 1.88 
                 0.23 
               
               
                   
                 CHCl 2M (μg/ml) 
                 0.41 
                 0.41 
                 2.24 
                 0.81 
               
               
                   
                 CHNM 2M (μg/ml) 
                 2.94 
                 4.88 
                 2.26 
                 1.77 
               
               
                   
                 AZT (μg/ml) 
                 5.11 
                 1.82 
                 1.07 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 K. K. Redda/ 
                   
                   
               
               
                 Chavor 
                   
                   
               
               
                 Exp.: 
                   
                 Nov. 14 to 
               
               
                 MCF-7 cells 
                 Cytoxicity on MCF-7 cells 
                 Nov. 18, 2005 
               
             
          
           
               
                 Drugs 
                 Cells (×10 4 ) 
                 Mean 
                 SD 
                 % Inhibition 
                 Mean 
                 SD 
               
               
                   
               
             
          
           
               
                   
                 56.8 
                   
                   
                   
                   
                   
               
               
                 2% DMSO 
                 61.8 
                 57.83 
                 2.86 
                 0 
                   
                   
               
               
                   
                 65.0 
                   
                   
                   
                   
                   
               
               
                 AZT 
                 53.0 
                   
                   
                 8.36 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 55.3 
                 53.83 
                 1.01 
                 4.47 
                 6.92 
                 1.74 
               
               
                   
                 53.3 
                   
                   
                 7.93 
                   
                   
               
               
                   
                 34.8 
                   
                   
                 39.91 
                   
                   
               
               
                 5 
                 34.5 
                 35.50 
                 1.24 
                 40.35 
                 38.62 
                 2.15 
               
               
                   
                 37.3 
                   
                   
                 35.59 
                   
                   
               
               
                   
                 22.3 
                   
                   
                 61.53 
                   
                   
               
               
                 10  
                 23.0 
                 22.50 
                 0.35 
                 60.23 
                 61.10 
                 0.61 
               
               
                   
                 22.3 
                   
                   
                 61.53 
                   
                   
               
               
                 CHBr 2M 
                 48.6 
                   
                   
                 15.71 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 51.5 
                 49.08 
                 1.85 
                 10.95 
                 15.13 
                 3.20 
               
               
                   
                 47.0 
                   
                   
                 18.73 
                   
                   
               
               
                   
                 27.0 
                   
                   
                 53.31 
                   
                   
               
               
                 5 
                 25.3 
                 26.25 
                 0.74 
                 56.34 
                 54.61 
                 1.27 
               
               
                   
                 26.5 
                   
                   
                 54.18 
                   
                   
               
               
                   
                 7.3 
                   
                   
                 87.46 
                   
                   
               
               
                 10  
                 9.0 
                 7.92 
                 0.77 
                 84.44 
                 86.31 
                 1.34 
               
               
                   
                 7.5 
                   
                   
                 87.03 
                   
                   
               
               
                   
                 0.8 
                   
                   
                 98.70 
                   
                   
               
               
                 50  
                 1.0 
                 0.75 
                 0.20 
                 98.27 
                 98.70 
                 0.35 
               
               
                   
                 0.5 
                   
                   
                 99.14 
                   
                   
               
               
                 CHCl 2M 
                 49.8 
                   
                   
                 13.98 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 48.5 
                 49.42 
                 0.66 
                 16.14 
                 14.55 
                 1.13 
               
               
                   
                 50.0 
                   
                   
                 13.54 
                   
                   
               
               
                   
                 32.0 
                   
                   
                 44.67 
                   
                   
               
               
                 5 
                 31.5 
                 32.33 
                 0.85 
                 45.53 
                 44.09 
                 1.47 
               
               
                   
                 33.5 
                   
                   
                 42.07 
                   
                   
               
               
                   
                 10.0 
                   
                   
                 82.71 
                   
                   
               
               
                 10  
                 10.8 
                 10.42 
                 0.31 
                 81.41 
                 81.99 
                 0.54 
               
               
                   
                 10.5 
                   
                   
                 81.84 
                   
                   
               
               
                   
                 1.0 
                   
                   
                 98.27 
                   
                   
               
               
                 50  
                 0.5 
                 0.75 
                 0.20 
                 99.14 
                 98.70 
                 0.35 
               
               
                   
                 0.8 
                   
                   
                 98.70 
                   
                   
               
               
                 CHNM 2M 
                 53.0 
                   
                   
                 8.36 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 55.3 
                 53.92 
                 0.96 
                 4.47 
                 6.77 
                 1.67 
               
               
                   
                 53.5 
                   
                   
                 7.49 
                   
                   
               
               
                   
                 45.5 
                   
                   
                 21.33 
                   
                   
               
               
                 5 
                 45.0 
                 46.17 
                 1.31 
                 22.19 
                 20.17 
                 2.27 
               
               
                   
                 48.0 
                   
                   
                 17.00 
                   
                   
               
               
                   
                 40.5 
                   
                   
                 29.97 
                   
                   
               
               
                 10  
                 39.0 
                 39.75 
                 0.61 
                 32.56 
                 31.27 
                 1.06 
               
               
                   
                 39.8 
                   
                   
                 31.27 
                   
                   
               
               
                   
                 32.5 
                   
                   
                 43.80 
                   
                   
               
               
                 50  
                 34.5 
                 33.50 
                 0.82 
                 40.35 
                 42.07 
                 1.41 
               
               
                   
                 33.5 
                   
                   
                 42.07 
                   
                   
               
               
                 MCF-7 
                 Mean 
                   
                   
                   
                   
                   
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 K. K. Redda/ 
                   
                   
               
               
                 Chavor 
                   
                   
               
               
                 Exp.: 
                   
                 Nov. 11 to 
               
               
                 PC-3 Cells 
                 Cytoxicity on PC-3 cells 
                 Nov. 17, 2005 
               
             
          
           
               
                 Drugs 
                 Cells (×10 4 ) 
                 Mean 
                 SD 
                 % Inhibition 
                 Mean 
                 SD 
               
               
                   
               
             
          
           
               
                   
                 99.3 
                   
                   
                   
                   
                   
               
               
                 2% DMSO 
                 98.3 
                 98.00 
                 1.14 
                 0 
                   
                   
               
               
                   
                 96.5 
                   
                   
                   
                   
                   
               
               
                 AZT 
                 84.0 
                   
                   
                 14.29 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 85.5 
                 84.58 
                 0.66 
                 12.76 
                 13.69 
                 0.67 
               
               
                   
                 84.3 
                   
                   
                 14.03 
                   
                   
               
               
                   
                 70.5 
                   
                   
                 28.06 
                   
                   
               
               
                 5 
                 72.3 
                 17.33 
                 0.72 
                 26.28 
                 27.21 
                 0.73 
               
               
                   
                 71.3 
                   
                   
                 27.30 
                   
                   
               
               
                   
                 61.5 
                   
                   
                 37.24 
                   
                   
               
               
                 10  
                 61.3 
                 61.58 
                 0.31 
                 37.50 
                 37.16 
                 0.32 
               
               
                   
                 62.0 
                   
                   
                 36.73 
                   
                   
               
               
                 CHBr 2M 
                 73.3 
                   
                   
                 25.26 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 72.0 
                 70.92 
                 2.47 
                 26.53 
                 27.64 
                 2.52 
               
               
                   
                 67.5 
                   
                   
                 31.12 
                   
                   
               
               
                   
                 75.5 
                   
                   
                 22.96 
                   
                   
               
               
                 5 
                 68.0 
                 70.83 
                 3.32 
                 30.61 
                 27.72 
                 3.39 
               
               
                   
                 69.0 
                   
                   
                 29.50 
                   
                   
               
               
                   
                 77.5 
                   
                   
                 20.92 
                   
                   
               
               
                 10  
                 70.0 
                 72.25 
                 3.72 
                 28.57 
                 26.28 
                 3.80 
               
               
                   
                 69.3 
                   
                   
                 29.34 
                   
                   
               
               
                   
                 57.5 
                   
                   
                 41.33 
                   
                   
               
               
                 50  
                 53.0 
                 56.00 
                 2.12 
                 45.92 
                 42.86 
                 2.16 
               
               
                   
                 57.5 
                   
                   
                 41.33 
                   
                   
               
               
                 CHCl 2M 
                 74.3 
                   
                   
                 24.23 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 74.0 
                 73.42 
                 1.01 
                 24.49 
                 25.09 
                 1.03 
               
               
                   
                 72.0 
                   
                   
                 26.53 
                   
                   
               
               
                   
                 69.0 
                   
                   
                 29.59 
                   
                   
               
               
                 5 
                 69.5 
                 70.00 
                 1.08 
                 29.08 
                 28.57 
                 1.10 
               
               
                   
                 71.5 
                   
                   
                 27.04 
                   
                   
               
               
                   
                 68.0 
                   
                   
                 30.61 
                   
                   
               
               
                 10  
                 70.0 
                 69.58 
                 1.16 
                 28.57 
                 29.00 
                 1.18 
               
               
                   
                 70.8 
                   
                   
                 27.81 
                   
                   
               
               
                   
                 67.3 
                   
                   
                 31.88 
                   
                   
               
               
                 50  
                 65.5 
                 62.08 
                 6.11 
                 33.16 
                 36.65 
                 6.24 
               
               
                   
                 53.5 
                   
                   
                 45.41 
                   
                   
               
               
                 CHNM 2M 
                 88.5 
                   
                   
                 9.69 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 81.3 
                 89.08 
                 6.65 
                 17.09 
                 9.10 
                 6.78 
               
               
                   
                 97.5 
                   
                   
                 0.51 
                   
                   
               
               
                   
                 59.5 
                   
                   
                 39.29 
                   
                   
               
               
                 5 
                 61.8 
                 62.67 
                 3.03 
                 36.99 
                 36.05 
                 3.09 
               
               
                   
                 66.8 
                   
                   
                 31.89 
                   
                   
               
               
                   
                 62.5 
                   
                   
                 36.22 
                   
                   
               
               
                 10  
                 60.3 
                 62.92 
                 2.37 
                 38.52 
                 35.80 
                 2.41 
               
               
                   
                 66.0 
                   
                   
                 32.65 
                   
                   
               
               
                   
                 49.8 
                   
                   
                 49.23 
                   
                   
               
               
                 50  
                 52.5 
                 50.17 
                 1.76 
                 46.43 
                 48.81 
                 1.80 
               
               
                   
                 48.3 
                   
                   
                 50.77 
                   
                   
               
               
                 PC-3 
                 mean 
                   
                   
                   
                   
                   
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 Drug Inhibition on PC-3 cells 
               
             
          
           
               
                   
                 1 
                 5 
                 10 
                 50 
               
               
                   
                   
               
             
          
           
               
                   
                 Drugs (μg/ml) 
                   
                   
                   
                   
               
               
                   
                 CHBr 2M (μg/ml) 
                 47.1 
                 65.88 
                 78.04 
                 89.50 
               
               
                   
                 CHCl 2M (μg/ml) 
                 42.4 
                 73.76 
                 87.02 
                 96.96 
               
               
                   
                 CHNM 2M (μg/ml) 
                 27.9 
                 39.36 
                 45.03 
                 59.67 
               
               
                   
                 AZT (μg/ml) 
                 17.27 
                 43.23 
                 59.67 
               
               
                   
                 SD 
               
               
                   
                 Drugs 
               
               
                   
                 CHBr 2M (μg/ml) 
                 0.78 
                 2.25 
                 0.59 
                 1.09 
               
               
                   
                 CHCl 2M (μg/ml) 
                 2.11 
                 0.85 
                 0.52 
                 0.20 
               
               
                   
                 CHNM 2M (μg/ml) 
                 0.59 
                 1.37 
                 0.98 
                 2.18 
               
               
                   
                 AZT (μg/ml) 
                 0.39 
                 0.90 
                 1.41 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 Drug Inhibition on MCF-7 Cells 
               
             
          
           
               
                   
                 1 
                 5 
                 10 
                 50 
               
               
                   
                   
               
             
          
           
               
                   
                 Drugs (μg/ml) 
                   
                   
                   
                   
               
               
                   
                 CHBr 2M (μg/ml) 
                 15.13 
                 54.61 
                 86.31 
                 98.709 
               
               
                   
                 CHCl 2M (μg/ml) 
                 14.55 
                 44.09 
                 81.99 
                 98.70 
               
               
                   
                 CHNM 2M (μg/ml) 
                 6.77 
                 20.17 
                 31.27 
                 42.07 
               
               
                   
                 AZT (μg/ml) 
                 6.92 
                 38.62 
                 61.1 
               
               
                   
                 SD 
               
               
                   
                 Drugs 
               
               
                   
                 CHBr 2M (μg/ml) 
                 3.2 
                 1.27 
                 1.34 
                 0.35 
               
               
                   
                 CHCl 2M (μg/ml) 
                 1.13 
                 1.47 
                 0.54 
                 0.35 
               
               
                   
                 CHNM 2M (μg/ml) 
                 1.67 
                 2.27 
                 1.06 
                 1.14 
               
               
                   
                 AZT (μg/ml) 
                 1.74 
                 2.15 
                 0.61 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 K. K. Redda/ 
                   
                   
               
               
                 Chavor 
                   
                   
               
               
                 Exp.: 
                   
                 Sep. 26 to 
               
               
                 MCF-7 Cells 
                 Cytoxicity on MCF-7 cells 
                 Sep. 30, 2005 
               
             
          
           
               
                 Drugs 
                 Cells (×10 4 ) 
                 Mean 
                 SD 
                 % Inhibition 
                 Mean 
                 SD 
               
               
                   
               
             
          
           
               
                   
                 49.0 
                   
                   
                   
                   
                   
               
               
                 2% DMSO 
                 50.5 
                 49.58 
                 0.66 
                 0 
                   
                   
               
               
                   
                 49.3 
                   
                   
                   
                   
                   
               
               
                 AZT 
                 35.5 
                   
                   
                 28.40 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 36.8 
                 36.42 
                 0.66 
                 25.88 
                 26.55 
                 1.32 
               
               
                   
                 37.0 
                   
                   
                 25.38 
                   
                   
               
               
                   
                 24.8 
                   
                   
                 49.98 
                   
                   
               
               
                 5 
                 27.3 
                 26.35 
                 1.10 
                 45.04 
                 46.86 
                 2.22 
               
               
                   
                 27.0 
                   
                   
                 45.55 
                   
                   
               
               
                   
                 17.3 
                   
                   
                 65.21 
                   
                   
               
               
                 10  
                 18.3 
                 17.25 
                 0.82 
                 63.19 
                 65.21 
                 1.65 
               
               
                   
                 16.3 
                   
                   
                 67.23 
                   
                   
               
               
                 CM-091303 
                 35.8 
                   
                   
                 27.90 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 35.8 
                 36.17 
                 0.59 
                 27.90 
                 27.06 
                 1.19 
               
               
                   
                 37.0 
                   
                   
                 25.38 
                   
                   
               
               
                   
                 35.3 
                   
                   
                 28.91 
                   
                   
               
               
                 5 
                 36.8 
                 35.75 
                 0.71 
                 25.88 
                 27.90 
                 1.43 
               
               
                   
                 35.3 
                   
                   
                 28.91 
                   
                   
               
               
                   
                 29.8 
                   
                   
                 40.00 
                   
                   
               
               
                 10  
                 29.3 
                 30.17 
                 0.96 
                 41.01 
                 39.16 
                 1.95 
               
               
                   
                 31.5 
                   
                   
                 36.47 
                   
                   
               
               
                   
                 26.3 
                   
                   
                 47.06 
                   
                   
               
               
                 50  
                 26.8 
                 26.17 
                 0.51 
                 46.5 
                 47.23 
                 1.04 
               
               
                   
                 25.5 
                   
                   
                 48.57 
                   
                   
               
               
                 CM-090803 
                 33.8 
                   
                   
                 31.93 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 33.5 
                 33.33 
                 0.42 
                 32.44 
                 32.77 
                 0.86 
               
               
                   
                 32.8 
                   
                   
                 33.95 
                   
                   
               
               
                   
                 26.0 
                   
                   
                 47.56 
                   
                   
               
               
                 5 
                 29.3 
                 28.17 
                 1.53 
                 41.01 
                 43.19 
                 3.09 
               
               
                   
                 29.3 
                   
                   
                 41.01 
                   
                   
               
               
                   
                 28.8 
                   
                   
                 42.02 
                   
                   
               
               
                 10  
                 28.3 
                 28.58 
                 0.24 
                 43.03 
                 42.35 
                 0.48 
               
               
                   
                 28.8 
                   
                   
                 42.02 
                   
                   
               
               
                   
                 23.3 
                   
                   
                 53.11 
                   
                   
               
               
                 50  
                 24.0 
                 23.75 
                 0.35 
                 51.60 
                 52.10 
                 0.71 
               
               
                   
                 24.0 
                   
                   
                 51.60 
                   
                   
               
               
                 CM-110104 
                 34.0 
                   
                   
                 31.43 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 35.0 
                 34.42 
                 0.42 
                 29.41 
                 30.59 
                 0.86 
               
               
                   
                 34.3 
                   
                   
                 30.92 
                   
                   
               
               
                   
                 27.5 
                   
                   
                 44.54 
                   
                   
               
               
                 5 
                 28.5 
                 28.08 
                 0.42 
                 42.52 
                 43.36 
                 0.86 
               
               
                   
                 28.3 
                   
                   
                 43.03 
                   
                   
               
               
                   
                 28.5 
                   
                   
                 42.52 
                   
                   
               
               
                 10  
                 27.0 
                 27.92 
                 0.66 
                 45.55 
                 43.70 
                 1.32 
               
               
                   
                 28.3 
                   
                   
                 43.03 
                   
                   
               
               
                   
                 19.8 
                   
                   
                 60.17 
                   
                   
               
               
                 50  
                 20.0 
                 19.87 
                 0.31 
                 59.66 
                 60.34 
                 0.63 
               
               
                   
                 19.3 
                   
                   
                 61.18 
                   
                   
               
               
                 MCF-7 
                 Mean 
                   
                   
                   
                   
                   
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 Drug Inhibition on PC-3 Cells 
               
             
          
           
               
                   
                 1 
                 5 
                 10 
                 50 
               
               
                   
                   
               
             
          
           
               
                   
                 Drugs (μg/ml) 
                   
                   
                   
                   
               
               
                   
                 CM-091303 (μg/ml) 
                 27.64 
                 27.72 
                 26.28 
                 42.86 
               
               
                   
                 CM-090803 (μg/ml) 
                 25.09 
                 28.57 
                 29 
                 36.65 
               
               
                   
                 CM-110104 (μg/ml) 
                 9.1 
                 36.05 
                 35.8 
                 48.81 
               
               
                   
                 AZT (μg/ml) 
                 13.69 
                 27.21 
                 37.16 
               
               
                   
                 SD 
               
               
                   
                 Drugs 
               
               
                   
                 CM-091303 (μg/ml) 
                 2.52 
                 3.39 
                 3.8 
                 2.16 
               
               
                   
                 CM-090803 (μg/ml) 
                 1.03 
                 1.1 
                 1.18 
                 6.24 
               
               
                   
                 CM-110104 (μg/ml) 
                 6.78 
                 3.09 
                 2.41 
                 1.80 
               
               
                   
                 AZT (μg/ml) 
                 0.67 
                 0.73 
                 0.32 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 11 
               
             
             
               
                   
               
               
                 K. K. Redda/ 
                   
                   
               
               
                 Chavor 
                   
                   
               
               
                 Exp.: 
                   
                 Sep. 12 to 
               
               
                 MCF-7 Cells 
                 Cytoxicity on MCF-7 cells 
                 Sep. 16, 2005 
               
             
          
           
               
                 Drugs 
                 Cells (x10 4 ) 
                 Mean 
                 SD 
                 % Inhibition 
                 Mean 
                 SD 
               
               
                   
               
             
          
           
               
                   
                 60.5 
                   
                   
                   
                   
                   
               
               
                 2% DMSO 
                 55.5 
                 57.67 
                 2.09 
                 0 
                   
                   
               
               
                   
                 57.0 
                   
                   
                   
                   
                   
               
               
                 AZT 
                 44.5 
                   
                   
                 22.83 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 46.0 
                 45.25 
                 0.61 
                 20.23 
                 21.53 
                 1.06 
               
               
                   
                 45.3 
                   
                   
                 21.53 
                   
                   
               
               
                   
                 30.0 
                   
                   
                 47.98 
                   
                   
               
               
                 5 
                 27.8 
                 28.58 
                 1.01 
                 51.88 
                 50.43 
                 1.75 
               
               
                   
                 28.0 
                   
                   
                 51.45 
                   
                   
               
               
                   
                 17.5 
                   
                   
                 69.65 
                   
                   
               
               
                 10  
                 21.5 
                 20.17 
                 1.89 
                 62.72 
                 65.03 
                 3.27 
               
               
                   
                 21.5 
                   
                   
                 62.72 
                   
                   
               
               
                 M/R-63-1 
                 33.3 
                   
                   
                 42.34 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 32.8 
                 32.17 
                 1.20 
                 43.21 
                 44.22 
                 2.07 
               
               
                   
                 30.5 
                   
                   
                 47.11 
                   
                   
               
               
                   
                 27.3 
                   
                   
                 52.75 
                   
                   
               
               
                 5 
                 27.5 
                 28.42 
                 1.48 
                 52.31 
                 50.72 
                 2.56 
               
               
                   
                 39.5 
                   
                   
                 47.11 
                   
                   
               
               
                   
                 30.8 
                   
                   
                 46.68 
                   
                   
               
               
                 10  
                 29.3 
                 29.25 
                 1.22 
                 49.28 
                 49.28 
                 2.12 
               
               
                   
                 27.8 
                   
                   
                 51.88 
                   
                   
               
               
                   
                 24.3 
                   
                   
                 57.95 
                   
                   
               
               
                 50  
                 23.5 
                 23.58 
                 0.51 
                 59.25 
                 59.10 
                 0.89 
               
               
                   
                 23.0 
                   
                   
                 60.12 
                   
                   
               
               
                 M/R-67 
                 39.3 
                   
                   
                 31.94 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 38.8 
                 39.67 
                 0.96 
                 32.80 
                 31.21 
                 1.67 
               
               
                   
                 41.0 
                   
                   
                 28.90 
                   
                   
               
               
                   
                 38.3 
                   
                   
                 33.67 
                   
                   
               
               
                 5 
                 37.5 
                 37.67 
                 0.42 
                 34.97 
                 34.68 
                 0.74 
               
               
                   
                 37.3 
                   
                   
                 35.40 
                   
                   
               
               
                   
                 36.0 
                   
                   
                 37.67 
                   
                   
               
               
                 10  
                 35.8 
                 35.17 
                 1.01 
                 38.01 
                 39.02 
                 1.75 
               
               
                   
                 33.8 
                   
                   
                 41.47 
                   
                   
               
               
                   
                 30.5 
                   
                   
                 47.11 
                   
                   
               
               
                 50  
                 31.5 
                 30.60 
                 0.82 
                 45.8 
                 47.11 
                 1.42 
               
               
                   
                 29.5 
                   
                   
                 48.84 
                   
                   
               
               
                 M/R-64-1 
                 30.8 
                   
                   
                 46.68 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 27.5 
                 29.08 
                 1.33 
                 52.31 
                 49.57 
                 2.30 
               
               
                   
                 29.0 
                   
                   
                 49.71 
                   
                   
               
               
                   
                 29.0 
                   
                   
                 49.71 
                   
                   
               
               
                 5 
                 28.0 
                 29.00 
                 0.82 
                 51.45 
                 49.71 
                 1.42 
               
               
                   
                 30.0 
                   
                   
                 47.98 
                   
                   
               
               
                   
                 27.0 
                   
                   
                 53.18 
                   
                   
               
               
                 10  
                 30.0 
                 29.08 
                 1.48 
                 47.98 
                 49.57 
                 2.56 
               
               
                   
                 30.3 
                   
                   
                 47.54 
                   
                   
               
               
                   
                 29.8 
                   
                   
                 48.41 
                   
                   
               
               
                 50  
                 28.0 
                 29.00 
                 0.74 
                 51.46 
                 49.71 
                 1.28 
               
               
                   
                 29.3 
                   
                   
                 49.28 
                   
                   
               
               
                 M/R-68-1 
                 50.3 
                   
                   
                 12.86 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 12 
               
             
             
               
                   
               
               
                 Drug Inhibition on MCF-7 Cells 
               
             
          
           
               
                   
                 1 
                 5 
                 10 
                 50 
               
               
                   
                   
               
             
          
           
               
                   
                 Drugs (μg/ml) 
                   
                   
                   
                   
               
               
                   
                 CM-091303 (μg/ml) 
                 27.06 
                 27.9 
                 39.16 
                 47.23 
               
               
                   
                 CM-090803 (μg/ml) 
                 32.77 
                 43.19 
                 42.35 
                 52.10 
               
               
                   
                 CM-110104 (μg/ml) 
                 30.59 
                 43.36 
                 43.7 
                 60.34 
               
               
                   
                 AZT (μg/ml) 
                 26.55 
                 46.86 
                 65.21 
               
               
                   
                 SD 
               
               
                   
                 Drugs 
               
               
                   
                 CM-091303 (μg/ml) 
                 1.19 
                 1.43 
                 1.95 
                 1.04 
               
               
                   
                 CM-090803 (μg/ml) 
                 0.86 
                 3.09 
                 0.48 
                 0.71 
               
               
                   
                 CM-110104 (μg/ml) 
                 0.86 
                 0.86 
                 1.32 
                 0.63 
               
               
                   
                 AZT (μg/ml) 
                 1.32 
                 2.22 
                 1.63 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 13 
               
               
                   
               
             
             
               
                 K. K. Redda/ 
                   
                   
               
               
                 Chavor 
                   
                   
               
               
                 Exp.: 
                   
                 Sep. 08 to 
               
               
                 MCF-7 Cells 
                 Cytoxicity on MCF-7 cells 
                 Sep. 12, 2005 
               
             
          
           
               
                 Drugs 
                 Cells (×10 4 ) 
                 Mean 
                 SD 
                 % Inhibition 
                 Mean 
                 SD 
               
               
                   
               
               
                   
                 74.3 
                   
                   
                   
                   
                   
               
               
                 2% DMSO 
                 68.5 
                 70.50 
                 2.65 
                 0 
                   
                   
               
               
                   
                 68.8 
                   
                   
                   
                   
                   
               
               
                 AZT 
                 51.0 
                   
                   
                 27.66 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 53.8 
                 52.33 
                 1.12 
                 23.76 
                 25.77 
                 1.59 
               
               
                   
                 52.3 
                   
                   
                 25.89 
                   
                   
               
               
                   
                 42.3 
                   
                   
                 40.07 
                   
                   
               
               
                 5 
                 42.3 
                 42.75 
                 0.71 
                 40.07 
                 39.36 
                 1.00 
               
               
                   
                 43.8 
                   
                   
                 37.94 
                   
                   
               
               
                   
                 30.0 
                   
                   
                 57.45 
                   
                   
               
               
                 10  
                 30.0 
                 30.00 
                 0.00 
                 57.45 
                 57.45 
                 0.00 
               
               
                   
                 30.0 
                   
                   
                 57.45 
                   
                   
               
               
                 M/R-67 
                 46.8 
                   
                   
                 33.69 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 43.8 
                 44.92 
                 1.31 
                 37.94 
                 36.29 
                 1.86 
               
               
                   
                 44.3 
                   
                   
                 37.23 
                   
                   
               
               
                   
                 41.8 
                   
                   
                 40.78 
                   
                   
               
               
                 5 
                 37.0 
                 39.75 
                 2.01 
                 47.52 
                 43.62 
                 2.85 
               
               
                   
                 40.5 
                   
                   
                 42.55 
                   
                   
               
               
                   
                 33.3 
                   
                   
                 52.84 
                   
                   
               
               
                 10  
                 34.8 
                 34.33 
                 0.77 
                 50.71 
                 51.30 
                 1.10 
               
               
                   
                 35.0 
                   
                   
                 50.35 
                   
                   
               
               
                   
                 38.5 
                   
                   
                 45.39 
                   
                   
               
               
                 50  
                 41.8 
                 40.67 
                 1.53 
                 40.78 
                 42.32 
                 2.17 
               
               
                   
                 41.8 
                   
                   
                 40.78 
                   
                   
               
               
                 M/R-68-1 
                 62.3 
                   
                   
                 11.70 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 55.8 
                 59.75 
                 2.86 
                 20.92 
                 15.25 
                 4.05 
               
               
                   
                 61.3 
                   
                   
                 13.12 
                   
                   
               
               
                   
                 51.0 
                   
                   
                 27.66 
                   
                   
               
               
                 5 
                 44.3 
                 47.92 
                 2.79 
                 37.23 
                 32.03 
                 3.95 
               
               
                   
                 48.5 
                   
                   
                 31.21 
                   
                   
               
               
                   
                 49.5 
                   
                   
                 29.79 
                   
                   
               
               
                 10  
                 51.0 
                 51.42 
                 1.76 
                 27.66 
                 27.07 
                 2.50 
               
               
                   
                 53.8 
                   
                   
                 23.76 
                   
                   
               
               
                   
                 38.8 
                   
                   
                 45.04 
                   
                   
               
               
                 50  
                 36.3 
                 38.92 
                 2.25 
                 48.58 
                 44.80 
                 3.19 
               
               
                   
                 41.8 
                   
                   
                 40.78 
               
               
                   
               
             
          
           
               
                   
                   
                 Mean 
                   
                   
                   
                   
               
               
                   
                 Drugs (μg/ml) 
                 0 
                 1 
                 5 
                 10 
                 50 
               
               
                   
               
               
                   
                 MCF-7 
                   
                   
                   
                   
                   
               
               
                   
                 AZT 
                   
                 25.77 
                 39.36 
                 57.45 
                   
               
               
                   
                 M/R-67 
                   
                 36.29 
                 43.62 
                 51.3 
                 42.32 
               
               
                   
                 M/R-68-1 
                   
                 15.25 
                 32.03 
                 27.07 
                 44.80 
               
               
                   
                 SD 
                   
                   
                   
                   
                   
               
               
                   
                 AZT 
                   
                 1.59 
                 1 
                 0 
                   
               
               
                   
                 M/R-67 
                   
                 1.86 
                 2.85 
                 1.1 
                 2.17 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 14 
               
               
                   
               
               
                 Drug inhibition on MCF-7 Cells 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 1 
                 54.0 
                 50.75 
                 2.47 
                 6.36 
                 11.99 
                 4.29 
               
               
                   
                 48.0 
                   
                   
                 16.76 
                   
                   
               
               
                   
                 37.5 
                   
                   
                 34.97 
                   
                   
               
               
                 5 
                 40.8 
                 39.33 
                 1.36 
                 29.34 
                 31.79 
                 2.36 
               
               
                   
                 39.8 
                   
                   
                 31.07 
                   
                   
               
               
                   
                 36.3 
                   
                   
                 37.14 
                   
                   
               
               
                 10 
                 33.8 
                 34.83 
                 1.05 
                 41.47 
                 39.60 
                 1.82 
               
               
                   
                 34.5 
                   
                   
                 40.17 
                   
                   
               
               
                   
                 31.3 
                   
                   
                 45.81 
                   
                   
               
               
                 50 
                 32.5 
                 31.92 
                 0.51 
                 43.64 
                 44.65 
                 0.89 
               
               
                   
                 32.0 
                   
                   
                 44.51 
               
               
                   
               
             
          
           
               
                   
                   
                 Mean 
                   
                   
                   
                   
               
               
                   
                 Drugs (μg/ml) 
                 0 
                 1 
                 5 
                 10 
                 50 
               
               
                   
               
               
                   
                 MCF-7 
                   
                   
                   
                   
                   
               
               
                   
                 M/R-67 
                   
                 31.21 
                 34.68 
                 39.02 
                 47.11 
               
               
                   
                 M/R-64-1 
                   
                 49.57 
                 49.71 
                 49.57 
                 49.71 
               
               
                   
                 M/R-68-1 
                   
                 11.99 
                 31.79 
                 39.6 
                 44.65 
               
               
                   
                 M/R-63-1 
                   
                 44.22 
                 50.72 
                 49.28 
                 59.10 
               
               
                   
                 AZT 
                   
                 21.53 
                 50.43 
                 65.03 
                   
               
               
                   
                 SD 
                   
                   
                   
                   
                   
               
               
                   
                 M/R-67 
                   
                 1.67 
                 0.74 
                 1.75 
                 1.42 
               
               
                   
                 M/R-64-1 
                   
                 2.3 
                 1.42 
                 2.56 
                 1.28 
               
               
                   
                 M/R-68-1 
                   
                 4.29 
                 2.36 
                 1.82 
                 0.89 
               
               
                   
                 M/R-63-1 
                   
                 2.07 
                 2.56 
                 2.12 
                 0.89 
               
               
                   
                 AZT 
                   
                 1.06 
                 1.56 
                 3.27 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
               
               
               
               
             
           
               
                 TABLE 15 
               
               
                   
               
             
             
               
                   
                 M/R-68-1 
                 4.05 
                 3.95 
                 2.5 
                 3.19 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 16 
               
               
                   
               
             
             
               
                 K. K. Redda/ 
                   
                   
               
               
                 Chavor 
                   
                   
               
               
                 Exp.: 
                   
                 Dec. 12 to 
               
               
                 MCF-7 Cells 
                 Cytoxicity on MCF-7 cells 
                 Dec. 16, 2005 
               
             
          
           
               
                 Drugs 
                 Cells (×10 4 ) 
                 Mean 
                 SD 
                 % Inhibition 
                 Mean 
                 SD 
               
               
                   
               
               
                   
                 50.3 
                   
                   
                   
                   
                   
               
               
                 2% DMSO 
                 52.0 
                 50.92 
                 0.77 
                 0 
                   
                   
               
               
                   
                 50.5 
                   
                   
                   
                   
                   
               
               
                 AZT 
                 43.3 
                   
                   
                 15.06 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 43.3 
                 43.08 
                 0.24 
                 15.06 
                 15.38 
                 0.46 
               
               
                   
                 42.8 
                   
                   
                 16.04 
                   
                   
               
               
                   
                 31.5 
                   
                   
                 38.13 
                   
                   
               
               
                 5 
                 32.3 
                 32.08 
                 0.42 
                 36.66 
                 36.99 
                 0.83 
               
               
                   
                 32.5 
                   
                   
                 36.17 
                   
                   
               
               
                   
                 22.0 
                   
                   
                 56.79 
                   
                   
               
               
                 10 
                 25.5 
                 24.08 
                 1.50 
                 49.92 
                 52.70 
                 2.96 
               
               
                   
                 24.8 
                   
                   
                 51.39 
                   
                   
               
               
                 CHCl 3M 
                 54.0 
                   
                   
                 −6.06 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 54.3 
                 53.17 
                 1.36 
                 −6.55 
                 −4.42 
                 2.67 
               
               
                   
                 51.3 
                   
                   
                 −0.65 
                   
                   
               
               
                   
                 53.8 
                   
                   
                 −5.56 
                   
                   
               
               
                 5 
                 51.5 
                 52.75 
                 0.94 
                 −1.15 
                 −3.60 
                 1.84 
               
               
                   
                 50.0 
                   
                   
                 −4.09 
                   
                   
               
               
                   
                 42.5 
                   
                   
                 16.53 
                   
                   
               
               
                 10 
                 43.3 
                 43.75 
                 1.27 
                 15.06 
                 14.08 
                 2.50 
               
               
                   
                 45.5 
                   
                   
                 10.64 
                   
                   
               
               
                   
                 32.0 
                   
                   
                 37.15 
                   
                   
               
               
                 50 
                 32.8 
                 31.50 
                 1.27 
                 35.68 
                 38.13 
                 2.50 
               
               
                   
                 29.8 
                   
                   
                 41.57 
                   
                   
               
               
                 CHNM 3M 
                 44.3 
                   
                   
                 13.09 
                   
                   
               
               
                 (μg/ml) 
                   
                   
                   
                   
                   
                   
               
               
                 1 
                 45.0 
                 44.50 
                 0.35 
                 11.62 
                 12.60 
                 0.69 
               
               
                   
                 44.3 
                   
                   
                 13.09 
                   
                   
               
               
                   
                 25.8 
                   
                   
                 49.43 
                   
                   
               
               
                 5 
                 24.0 
                 24.75 
                 0.74 
                 52.86 
                 51.39 
                 1.45 
               
               
                   
                 24.5 
                   
                   
                 51.88 
                   
                   
               
               
                   
                 5.0 
                   
                   
                 90.18 
                   
                   
               
               
                 10 
                 4.8 
                 4.92 
                 0.12 
                 90.67 
                 90.34 
                 0.23 
               
               
                   
                 5.0 
                   
                   
                 90.18 
                   
                   
               
               
                   
                 1.8 
                   
                   
                 96.56 
                   
                   
               
               
                 50 
                 2.0 
                 1.75 
                 0.20 
                 96.07 
                 96.56 
                 0.40 
               
               
                   
                 1.5 
                   
                   
                 97.05 
               
               
                   
               
             
          
           
               
                   
                 Drugs (μg/ml) 
                 1 
                 5 
                 10 
                 50 
               
               
                   
               
               
                   
                 MCF-7 
                   
                   
                   
                   
               
               
                   
                 CHCl 3M 
                 −4.42 
                 −3.6 
                 14.08 
                 38.13 
               
               
                   
                 CHNM 3M 
                 12.6 
                 51.39 
                 90.34 
                 96.66 
               
               
                   
                 AZT 
                 15.38 
                 36.99 
                 52.7 
                   
               
               
                   
                 SD 
                   
                   
                   
                   
               
               
                   
                 CHCl 3M 
                 2.67 
                 1.84 
                 2.5 
                 2.50 
               
               
                   
                 CHNM 3M 
                 0.69 
                 1.45 
                 0.23 
                 0.40 
               
               
                   
                 AZT 
                 0.46 
                 0.83 
                 2.96 
               
               
                   
               
             
          
         
       
     
     Examining the response of the synthesized compounds to MCF-7 cells in the different drug concentrations reveals that the compounds of the invention, in particular the three (code: CHBr2M, CHCl2M and CHNM3M) show good activities. These three congeners were further evaluated in H9 and PC3 cells. AZT was employed as a positive control in all of these experiments. These data are summarized in  FIGS. 1-3 . The results show that the compounds of the invention, in particular the two [CHBr2M and CHCl2M] are as good or somewhat better in inhibiting the growth in all the cultures employed. 
     It has been demonstrated previously that the above disclosed tests are indicative of anti-viral, anti-retroviral and, in particular, anti-HIV properties. See Mehta R, Lansky, Eur J Cancer Prev. 2004 August;13(4):345-8; Pei-Lin Wu , Yu-Lin Hsu , Tian-Shung Wu , K Bastow , Kuo-Hsiung Lee, “Kalanchosides A-C, New Cytotoxic Bufadienolides from the Aerial Parts of Kalanchoe gracilis”, Org Lett. 2006 Nov. 9;8 (23):5207-5210 17078679; Casini et al, Environmental Health Perspectives, vol. 1 10, pp801-805 (2002) and Bioorg Med Chem. 2001 November;9(11):2871-84.