Abstract:
A compound of general formula (I), wherein R 1 -R 17  and the ------ line take various meanings for use in the treatment of cancer.

Description:
FIELD OF THE INVENTION 
       [0001]    The present invention relates to new anticancer compounds, pharmaceutical compositions containing them, and their use as anticancer agents. 
       BACKGROUND OF THE INVENTION 
       [0002]    The present invention relates to compounds with some structural similarities to bufadienolide compounds disclosed in the prior art. For a review of bufadienolides see Huimin Gao et al in Nat. Prod. Rep., 2011, 28, 953. 
         [0003]    The bufadienolide compounds reported in the prior art are natural steroids, originally isolated from terrestrial natural sources such as plants of the families Crassulaceae, Hyacinthaceae, Iridaceae, Melianthaceae, Ranunculaceae, and Santalaceaethe, and animals of the genus  Bufo  (toads),  Photinus  (fireflies), and  Rhabdophis  (snakes) (Steyn et al. Nat. Prod. Rep. 1998, 15, 397-413; Krenn et al. Phytochemistry, 1998, 48(1), 1-29). 
         [0004]    Among these bufadienolide compounds, Scilliroside and other Scilla compounds have been isolated from the red squill,  Urginea maritima , and are disclosed to be highly toxic, specially Scilliroside which affects the cardiovascular and central nervous systems, causing convulsions and death (Verbiscar et al. J. Agric. Food Chem. 1986, 34, 973-979; Kopp et al. Phytochemistry, 1996, 42(2), 513-522). Majinda et al. also isolated bufadienolide compounds from  Urginea sanguinea , which make the plant unsafe to be used as a medicinal plant (Planta Med. 1997, 63, 188-190). 
         [0005]    The antiviral activity against a series of rhinoviruses and the anti-herpetic activity of several bufadienolide compounds were evaluated by Kamano et al. (Chem. Pharm. Bull. 1988, 36(1), 326-332) and Takechi et al. (Phytochemistry, 1996, 41(1), 125-127), respectively, and they found that most of the compounds displayed some inhibitory activity. 
         [0006]    Additionally, the cytotoxic activity of several bufadienolide compounds has been evaluated by several authors. Specifically, Jing et al. reported that bufalin has a potent growth-inhibitory effect on human cancer cells of leukaemia (HL-60, ML1, U937, and K562 cell lines), epithelioid carcinoma (HeLa cell line), hepatoma (PLC/PRF/5 cell line), and epidermoid carcinoma (A431 cell line), but it is less potent on mouse leukaemia M1, melanoma B16, and lymphoid neoplasm P388 cell lines and rat hepatoma AH66 and chromaffin cell PC12 cell lines. They also found that bufalin induces typical apoptosis in human leukaemia HL-60 cell line but not in human leukocytes (Jpn. J. Cancer Res. 1994, 85(6), 645-651). 
         [0007]    Kupchan et al. disclosed several bufadienolide compounds isolated from  Bersama abyssinica  which showed inhibitory activity against human carcinoma of the nasopharynx (KB) cell line (Bioorg. Chem. 1971, 1, 13-31; J. Org. Chem. 1971, 36(18), 2611-2616). 
         [0008]    Kamano et al. evaluated the cytotoxic activity of 80 bufadienolide and cardenolide compounds, isolated from the Chinese drug Ch&#39;an Su (obtained from the skin glands of toads such as  Bufo gargarizans ), against a primary liver carcinoma PLC/PRF/5 cell line and the colchicine-resistant cell line of PLC/PRF/5. Of them, 16 were shown to have potent cytotoxicities (IC 50 &lt;10 −3  μg/mL) against PLC/PRF/5 cell line (Bioorg. Med. Chem. 1998, 6, 1103-1115; J. Med. Chem. 2002, 45, 5440-5447). Additional bufadienolide compounds were isolated by Nogawa et al. from the same source, which were tested against human carcinoma of the nasopharyx (KB), human leukaemia (HL-60), murine leukaemia (MH60), pancreas adenocarcinoma (BXPC3), breast adenocarcinoma (MCF7), CNS glioblastoma (SF268), lung NSC (NCIH460), colon carcinoma (KM20L2), and prostate cancer (DU145) cell lines (J. Nat. Prod. 2001, 64, 1148-1152). 
         [0009]    Ye et al. prepared novel bufadienolide compounds from bufalin by microbial hydroxylation. The compounds were tested against human hepatoma Bel-7402, human gastric cancer BGC-823, human cervical carcinoma HeLa, and human leukaemia HL-60 cell lines, showing some of them potent cytotoxicities comparable to that of bufalin (J. Steroid. Biochem. Mol. Biol. 2004, 91, 87-98; J. Nat. Prod. 2005, 68, 626-628). 
         [0010]    Since cancer is a leading cause of death in animals and humans, several efforts have been and are still being undertaken in order to obtain an anticancer therapy active and safe to be administered to patients suffering from a cancer. The problem to be solved by the present invention is to provide compounds that are useful in the treatment of cancer. 
       SUMMARY OF THE INVENTION 
       [0011]    In one aspect, the present invention is directed to compounds of general formula I or pharmaceutically acceptable salts, prodrugs or stereoisomers thereof 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein
 
each R 1  and R 2  is independently selected from hydrogen, halogen, OR a , OCOR a , and OCOOR a , or R 1  and R 2  together are ═O;
 
each R 3 , R 15 , and R 16  is independently selected from hydrogen, OR a , OCOR a , OCOOR a , and ═O, with the proviso that when a ═O group exists the hydrogen of the C atom to which the ═O is attached is absent;
 
each R 4 , R 5 , R 6 , R 7 , R 11 , R 12 , and R 14  is independently selected from hydrogen, OR a , OCOR a , and OCOOR a ;
 
each R 8 , R 9 , and R 17  is independently selected from hydrogen, OR a , OCOR a , OCOOR a , substituted or unsubstituted C 1 -C 12  alkyl, substituted or unsubstituted C 2 -C 12  alkenyl, and substituted or unsubstituted C 2 -C 12  alkynyl;
 
R 10  is selected from hydrogen, OR b , OCOR a , OCOOR a , and ═O, with the proviso that when a ═O group exists the hydrogen of the C atom to which the ═O is attached is absent;
 
R 13  is selected from hydrogen, COR a , substituted or unsubstituted C 1 -C 12  alkyl, substituted or unsubstituted C 2 -C 12  alkenyl, and substituted or unsubstituted C 2 -C 12  alkynyl;
 
each R a  is independently selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, substituted or unsubstituted C 2 -C 12  alkenyl, substituted or unsubstituted C 2 -C 12  alkynyl, substituted or unsubstituted aryl, and substituted or unsubstituted heterocyclic group;
 
each R b  is independently selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, substituted or unsubstituted C 2 -C 12  alkenyl, substituted or unsubstituted C 2 -C 12  alkynyl, substituted or unsubstituted aryl, substituted or unsubstituted heterocyclic group, and substituted or unsubstituted sugar; and
 
the           line represents an additional bond, an epoxy group, or is absent.
 
         [0012]    In another aspect, the present invention is directed to a compound of formula I, or a pharmaceutically acceptable salt, prodrug or stereoisomer thereof, for use as a medicament, in particular as a medicament for treating cancer. 
         [0013]    In a further aspect, the present invention is also directed to the use of a compound of formula I, or a pharmaceutically acceptable salt, prodrug or stereoisomer thereof, in the treatment of cancer, or in the preparation of a medicament, preferably for the treatment of cancer. Other aspects of the invention are methods of treatment, and compounds for use in these methods. Therefore, the present invention further provides a method of treating any mammal, notably a human, affected by cancer which comprises administering to the affected individual a therapeutically effective amount of a compound as defined above. 
         [0014]    In a yet further aspect, the present invention is also directed to a compound of formula I, or a pharmaceutically acceptable salt, prodrug or stereoisomer thereof, for use as an anticancer agent. 
         [0015]    In another aspect, the present invention is directed to pharmaceutical compositions comprising a compound of formula I, or a pharmaceutically acceptable salt, prodrug or stereoisomer thereof, together with a pharmaceutically acceptable carrier or diluent. 
     
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0016]    The present invention relates to compounds of general formula I as defined above. 
         [0017]    In these compounds the groups can be selected in accordance with the following guidance: 
         [0018]    Alkyl groups may be branched or unbranched, and preferably have from 1 to about 12 carbon atoms. One more preferred class of alkyl groups has from 1 to about 6 carbon atoms. Even more preferred are alkyl groups having 1, 2, 3 or 4 carbon atoms. Methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl are particularly preferred alkyl groups in the compounds of the present invention. As used herein, the term alkyl, unless otherwise stated, refers to both cyclic and noncyclic groups, although cyclic groups will comprise at least three carbon ring members. 
         [0019]    Preferred alkenyl and alkynyl groups in the compounds of the present invention may be branched or unbranched, have one or more unsaturated linkages and from 2 to about 12 carbon atoms. One more preferred class of alkenyl and alkynyl groups has from 2 to about 6 carbon atoms. Even more preferred are alkenyl and alkynyl groups having 2, 3 or 4 carbon atoms. The terms alkenyl and alkynyl as used herein refer to both cyclic and noncyclic groups, although cyclic groups will comprise at least three carbon ring members. 
         [0020]    Suitable aryl groups in the compounds of the present invention include single and multiple ring compounds, including multiple ring compounds that contain separate and/or fused aryl groups. Typical aryl groups contain from 1 to 3 separated or fused rings and from 6 to about 18 carbon ring atoms. Preferably aryl groups contain from 6 to about 10 carbon ring atoms. Specially preferred aryl groups include substituted or unsubstituted phenyl, substituted or unsubstituted naphthyl, substituted or unsubstituted biphenyl, substituted or unsubstituted phenanthryl, and substituted or unsubstituted anthryl. 
         [0021]    Suitable heterocyclic groups include heteroaromatic and heteroalicyclic groups containing from 1 to 3 separated and/or fused rings and from 5 to about 18 ring atoms. Preferably heteroaromatic and heteroalicyclic groups contain from 5 to about 10 ring atoms. Suitable heteroaromatic groups in the compounds of the present invention contain one, two or three heteroatoms selected from N, O or S atoms and include, e.g., coumarinyl including 8-coumarinyl, quinolyl including 8-quinolyl, isoquinolyl, pyridyl, pyrazinyl, pyrazolyl, pyrimidinyl, furyl, pyrrolyl, thienyl, thiazolyl, isothiazolyl, triazolyl, tetrazolyl, isoxazolyl, oxazolyl, imidazolyl, indolyl, isoindolyl, indazolyl, indolizinyl, phthalazinyl, pteridinyl, purinyl, oxadiazolyl, thiadiazolyl, furazanyl, pyridazinyl, triazinyl, cinnolinyl, benzimidazolyl, benzofuranyl, benzofurazanyl, benzothienyl, benzothiazolyl, benzoxazolyl, quinazolinyl, quinoxalinyl, naphthyridinyl and furopyridyl. Suitable heteroalicyclic groups in the compounds of the present invention contain one, two or three heteroatoms selected from N, O or S atoms and include, e.g., pyrrolidinyl, tetrahydrofuryl, dihydrofuryl, tetrahydrothienyl, tetrahydrothiopyranyl, piperidyl, morpholinyl, thiomorpholinyl, thioxanyl, piperazinyl, azetidinyl, oxetanyl, thietanyl, homopiperidyl, oxepanyl, thiepanyl, oxazepinyl, diazepinyl, thiazepinyl, 1,2,3,6-tetrahydropyridyl, 2-pyrrolinyl, 3-pyrrolinyl, indolinyl, 2H-pyranyl, 4H-pyranyl, dioxolanyl, dioxanyl, 1,3-dioxolanyl, pyrazolinyl, dithianyl, dithiolanyl, dihydropyranyl, dihydrothienyl, pyrazolidinyl, imidazolinyl, imidazolidinyl, 3-azabicyclo[3.1.0]hexyl, 3-azabicyclo[4.1.0]heptyl, 3H-indolyl, and quinolizinyl. 
         [0022]    The term sugar includes monosaccharides, disaccharides, trisaccharides, polysaccharides, oligosaccharides, and saccharide derivatives. Preferably the saccharide is selected from rhamnose, glucose, digitoxose, digitalose, digginose, sarmentose, vallarose, and fructose. Derivatives thereof, including sugar glycosides, N-glycosylamines, O-acyl derivatives, O-methyl derivatives, sugar alcohols, sugar acids, deoxy sugars, and related groups, are also preferred sugar groups. 
         [0023]    Suitable halogen groups in the compounds of the present invention include F, Cl, Br and I. 
         [0024]    The groups above mentioned may be substituted at one or more available positions by one or more suitable groups such as OR′, ═O, SR′, SOR′, SO 2 R′, NO 2 , NHR′, N(R′) 2 , ═N—R′, NHCOR′, N(COR′) 2 , NHSO 2 R′, NR′C(═NR′)NR′R′, CN, halogen, COR′, COOR′, OCOR′, OCONHR′, OCON(R′) 2 , CONHR′, CON(R′) 2 , substituted or unsubstituted C 1 -C 12  alkyl, substituted or unsubstituted C 2 -C 12  alkenyl, substituted or unsubstituted C 2 -C 12  alkynyl, substituted or unsubstituted aryl, and substituted or unsubstituted heterocyclic group, wherein each of the R′ groups is independently selected from the group consisting of hydrogen, OH, NO 2 , NH 2 , SH, CN, halogen, COH, COalkyl, COOH, substituted or unsubstituted C 1 -C 12  alkyl, substituted or unsubstituted C 2 -C 12  alkenyl, substituted or unsubstituted C 2 -C 12  alkynyl, substituted or unsubstituted aryl, and substituted or unsubstituted heterocyclic group. Where such groups are themselves substituted, the substituents may be chosen from the foregoing list. 
         [0025]    The term “pharmaceutically acceptable salts and prodrugs” refers to any pharmaceutically acceptable salt, ester, solvate, hydrate or any other compound which, upon administration to the patient is capable of providing (directly or indirectly) a compound as described herein. However, it will be appreciated that non-pharmaceutically acceptable salts also fall within the scope of the invention since those may be useful in the preparation of pharmaceutically acceptable salts. The preparation of salts and prodrugs can be carried out by methods known in the art. 
         [0026]    For instance, pharmaceutically acceptable salts of compounds provided herein are synthesized from the parent compound, which contains a basic or acidic moiety, by conventional chemical methods. Generally, such salts are, for example, prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent or in a mixture of both. Generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol or acetonitrile are preferred. Examples of the acid addition salts include mineral acid addition salts such as, for example, hydrochloride, hydrobromide, hydroiodide, sulphate, nitrate, phosphate, and organic acid addition salts such as, for example, acetate, trifluoroacetate, maleate, fumarate, citrate, oxalate, succinate, tartrate, malate, mandelate, methanesulfonate and p-toluenesulfonate. Examples of the alkali addition salts include inorganic salts such as, for example, sodium, potassium, calcium and ammonium salts, and organic alkali salts such as, for example, ethylenediamine, ethanolamine, N,N-dialkylenethanolamine, triethanolamine and basic aminoacids salts. 
         [0027]    The compounds of the invention may be in crystalline form either as free compounds or as solvates (e.g. hydrates) and it is intended that both forms are within the scope of the present invention. Methods of solvation are generally known within the art. 
         [0028]    Any compound that is a prodrug of a compound of formula I is within the scope of the invention. The term “prodrug” is used in its broadest sense and encompasses those derivatives that are converted in vivo to the compounds of the invention. Examples of prodrugs include, but are not limited to, derivatives and metabolites of the compounds of formula I or II that include biohydrolyzable moieties such as biohydrolyzable amides, biohydrolyzable esters, biohydrolyzable carbamates, biohydrolyzable carbonates, biohydrolyzable ureides, and biohydrolyzable phosphate analogues. Preferably, prodrugs of compounds with carboxyl functional groups are the lower alkyl esters of the carboxylic acid. The carboxylate esters are conveniently formed by esterifying any of the carboxylic acid moieties present on the molecule. Prodrugs can tipically be prepared using well-known methods, such as those described by Burger “Medicinal Chemistry and Drug Discovery 6th ed. (Donald J. Abraham ed., 2001, Wiley) and “Design and Applications of Prodrugs” (H. Bundgaard ed., 1985, Harwood Academic Publishers). 
         [0029]    Any compound referred to herein is intended to represent such specific compound as well as certain variations or forms. In particular, compounds referred to herein may have asymmetric centres and therefore exist in different enantiomeric or diastereoisomeric forms. Thus any given compound referred to herein is intended to represent any one of a racemate, one or more enantiomeric forms, one or more diastereomeric forms, and mixtures thereof. Likewise, stereoisomerism or geometric isomerism about the double bond is also possible, therefore in some cases the molecule could exist as (E)-isomer or (Z)-isomer (trans and cis isomers). If the molecule contains several double bonds, each double bond will have its own stereoisomerism, that could be the same as, or different to, the stereoisomerism of the other double bonds of the molecule. Furthermore, compounds referred to herein may exists as atropoisomers. All the stereoisomers including enantiomers, diastereoisomers, geometric isomers and atropoisomers of the compounds referred to herein, and mixtures thereof, are considered within the scope of the present invention. 
         [0030]    Furthermore, any compound referred to herein may exist in isotopically-labelled forms i.e. compounds which differ in the presence of one or more isotopically-enriched atoms. For example, compounds having the present structures except for the replacement of at least one hydrogen atom by deuterium or tritium, or the replacement of at least one carbon by  13 C— or  14 C-enriched carbon, or the replacement of at least one nitrogen atom by  15 N-enriched nitrogen are within the scope of this invention. 
         [0031]    To provide a more concise description, some of the quantitative expressions given herein are not qualified with the term “about”. It is understood that, whether the term “about” is used explicitly or not, every quantity given herein is meant to refer to the actual given value, and it is also meant to refer to the approximation to such given value that would reasonably be inferred based on the ordinary skill in the art, including equivalents and approximations due to the experimental and/or measurement conditions for such given value. 
         [0032]    In compounds of general formula I, each R 1  and R 2  is preferably and independently selected from hydrogen, halogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably, R 1  and R 2  are each independently selected from hydrogen and halogen, being Cl the most preferred halogen. 
         [0033]    Particular preferred R 3 , R 5 , R 6 , R 14 , and R 15  are each independently selected from hydrogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably R 3 , R 5 , R 6 , R 14 , and R 15  are hydrogen. 
         [0034]    R 4  is preferably selected from hydrogen and OR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably, R 4  is OR a  and R a  is hydrogen. 
         [0035]    R 7  is preferably selected from hydrogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably, R 7  is hydrogen. 
         [0036]    Particularly preferred R 8  and R 9  are each independently selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably R 8  and R 9  are each a substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred are methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl; being methyl the most preferred. 
         [0037]    R 10  is preferably selected from OR b , OCOR a , and ═O, with the proviso that when R 10  is ═O the hydrogen of the C atom to which R 10  is attached is absent, and wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl and R b  is selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, and substituted or unsubstituted sugar. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. Particularly preferred R b  is hydrogen, substituted or unsubstituted C 1 -C 6  alkyl, monosaccharide, disaccharide, and trisaccharide; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, tert-butyl, sec-butyl, isobutyl, rhamnose, glucose, digitoxose, digitalose, digginose, sarmentose, vallarose, and fructose. More preferably, R 10  is ═O or OR b , wherein R b  is methyl. 
         [0038]    In one preferred class of the compounds of the invention wherein the           line is absent, particularly preferred R 11  and R 12  are each independently selected from hydrogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. 
         [0039]    In another preferred class of compounds of the invention wherein either an additional bond or an epoxy group is present in the place indicated with the           line, particularly preferred R 11  and R 12  are hydrogen. 
         [0040]    R 13  is preferably selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, and COR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably R 13  is substituted or unsubstituted C 1 -C 6  alkyl and COR a , wherein R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl. Even more preferred R 13  is a substituted or unsubstituted methyl, substituted or unsubstituted ethyl, substituted or unsubstituted propyl, and COR a , wherein R a  is hydrogen. Preferred substituents of methyl, ethyl, and propyl are OR′ wherein R′ is hydrogen or COalkyl, being hydrogen the most preferred R′. 
         [0041]    R 16  is preferably selected from hydrogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably R 16  is hydrogen or OR a , wherein R a  is hydrogen. 
         [0042]    Particularly preferred R 17  is substituted or unsubstituted C 1 -C 12  alkyl. More preferably is a substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. Methyl is the most preferred R 17 . 
         [0043]    Particularly preferred is the presence of an additional bond or an epoxy group in the place indicated with the           line. 
         [0044]    More particularly, the invention provides compounds of general formula II or pharmaceutically acceptable salts, prodrugs or stereoisomers thereof 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein R 1 , R 2 , R 4 , R 7 , R 8 -R 13 , R 16  and the           line have the same meaning given above. 
         [0045]    Particularly preferred stereochemistry of said compounds of general formula II is the following: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0046]    In compounds of general formula II, each R 1  and R 2  is preferably and independently selected from hydrogen, halogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably, R 1  and R 2  are each independently selected from hydrogen and halogen, being Cl the most preferred halogen. 
         [0047]    R 4  is preferably selected from hydrogen and OR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably, R 4  is OR a  and R a  is hydrogen. 
         [0048]    R 7  is preferably selected from hydrogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably, R 7  is hydrogen. 
         [0049]    Particularly preferred R 8  and R 9  are each independently selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably R 8  and R 9  are each a substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred are methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl; being methyl the most preferred. 
         [0050]    R 10  is preferably selected from OR b , and OCOR a , and ═O, with the proviso that when R 10  is ═O the hydrogen of the C atom to which R 10  is attached is absent, and wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl and R b  is selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, and substituted or unsubstituted sugar. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. Particularly preferred R b  is hydrogen, substituted or unsubstituted C 1 -C 6  alkyl, monosaccharide, disaccharide, and trisaccharide; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, tert-butyl, sec-butyl, isobutyl, rhamnose, glucose, digitoxose, digitalose, digginose, sarmentose, vallarose, and fructose. More preferably, R 10  is ═O or OR b , wherein R b  is methyl. 
         [0051]    In one preferred class of the compounds of the invention wherein the           line is absent, particularly preferred R 11  and R 12  are each independently selected from hydrogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. 
         [0052]    In another preferred class of compounds of the invention wherein either an additional bond or an epoxy group is present in the place indicated with the           line, particularly preferred R 11  and R 12  are hydrogen. 
         [0053]    R 13  is preferably selected from hydrogen, substituted or unsubstituted C 1 -C 12  alkyl, and COR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably R 13  is substituted or unsubstituted C 1 -C 6  alkyl and COR a , wherein R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl. Even more preferred R 13  is a substituted or unsubstituted methyl, substituted or unsubstituted ethyl, substituted or unsubstituted propyl, and COR a , wherein R a  is hydrogen. Preferred substituents of methyl, ethyl, and propyl are OR′ wherein R′ is hydrogen or COalkyl, being hydrogen the most preferred R′. 
         [0054]    R 16  is preferably selected from hydrogen, OR a , and OCOR a , wherein R a  is selected from hydrogen and substituted or unsubstituted C 1 -C 12  alkyl. Particularly preferred R a  is hydrogen and substituted or unsubstituted C 1 -C 6  alkyl; and even more preferred is hydrogen, methyl, ethyl, n-propyl, isopropyl and butyl, including n-butyl, tert-butyl, sec-butyl and isobutyl. More preferably R 16  is hydrogen or OR a , wherein R a  is hydrogen. 
         [0055]    Particularly preferred is the presence of an additional bond or an epoxy group in the place indicated with a           line. 
         [0056]    In the present description and definitions, when there are several substituents R a  present in the compounds of the invention, and unless it is stated explicitly so, it should be understood that they can be each independently different within the given definition, i.e. R a  does not represent necessarily the same group simultaneously in a given compound of the invention. 
         [0057]    In the previous paragraphs preferences for the substituent groups R 1  to R 17  and the dotted line are defined. It should also be understood that the different combinations of these preferences are also preferred within the compounds of the invention. 
         [0058]    Particularly preferred compounds of the invention are the following: 
         [0000]    
       
                 
         
             
             
         
       
       
                 
         
             
             
         
       
     
         [0000]    or pharmaceutically acceptable salts, prodrugs or stereoisomers thereof. The stereochemistry indicated here for Aegomycins A-His relative. 
         [0059]    Aegomycins A-H were isolated from a porifera of the family Mycalidae, genus  Mycale , subgenus  Aegogropila , species  Mycale  ( Aegogropila )  crassissima  (Dendy, 1905). A sample of  Mycale  ( Aegogropila )  crassissima  (Dendy, 1905) was deposited at the Institute of Marine Sciences and Limnology of the Universidad Nacional Autónoma of Mexico, with the reference code MHA-399. This sponge was collected by hand using SCUBA diving in Mafia Island (07° 39.558′ S/39° 55.043′ E) at depths ranging between 5 and 31.4 m. 
         [0060]    The description of the sponge is the following: The specimens examined were thinly encrusting growing on rocks. The colour of live specimens was brown, although they have also been described as orange or ochre. Spicule complement: Megascleres were straight subtylostyles, often with hardly perceptible tyle, and usually slightly bent in the middle. They had about 270 μm of mean length, about 4.6 μm of shaft width and about 4.8 μm of tyle width. Microscleres included palmate anisochelae (three size classes: Anisochela 1 of about 36 μm in average, anisochela 2 of about 27 μm in average, and anisochela 3 of about 11 μm in average), sigmas (one class: Robust sigma of about 57 μm in average), and raphides (very scarce). Skeletal arrangement: Tracts of subtylostyles ascended outwards from the basal plate and terminated at the surface in slight tufts or brushes. Ectosome had a well developed reticulation of bundles of mycalostyles and microscleres were strewn at random in both choanosome and ectosome. The anisochelae did not form rosettes.  Mycale crassissima  is common in the collected area of Mafia Island and has also been found in Mombassa, Zanzibar, Madagascar, Ceylon, and Arafura Sea, on rocks and coral reefs, from 1 to 60 m depth. 
         [0061]    Additionally, compounds of the invention can be obtained by synthesis following usual procedures in synthetic organic chemistry and already known by a person skilled in the art. For example, compounds of this invention can be obtained adapting the procedures described in the literature such as in Steyn et al. Nat. Prod. Rep. 1998, 15, 397-413; Huimin Gao et al in Nat. Prod. Rep., 2011, 28, 953; WO 01/79256; WO 2006/120472; and CA 2.418.458. The synthetic routes can use combinations of steps taken from more than one of these references. 
         [0062]    Likewise, natural, synthetic or already modified compounds of the invention can be further modified by a variety of chemical reaction to obtain additional compounds of the invention. Thus, hydroxyl groups can be acylated by standard coupling or acylation procedures, for instance by using acetic acid or acetic anhydride in pyridine or the like. Formate groups can be obtained by heating hydroxyl precursors in formic acid. Hydroxy groups can also be oxidized to oxo (═O), for instance, by using manganese dioxide or chromium, or converted into amino-lower alkoxy, for instance, by using a protected 2-bromoethylamine. Carboxy groups can be alkylated, for instance, methylated by treatment with diazomethane. Glycosidic moieties can be introduced by standard sugar coupling reactions. 
         [0063]    An important feature of the above described compounds of formula I and II is their bioactivity and in particular their cytotoxic activity. 
         [0064]    With this invention we provide novel pharmaceutical compositions of compounds of general formula I and II that possess cytotoxic activity and their use as anticancer agents. Thus the present invention further provides pharmaceutical compositions comprising a compound of this invention, or a pharmaceutically acceptable salt, prodrug or stereoisomer thereof with a pharmaceutically acceptable carrier. 
         [0065]    Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, granules etc.) or liquid (solutions, suspensions or emulsions) composition for oral, topical or parenteral administration. 
         [0066]    Administration of the compounds or compositions of the present invention may be by any suitable method, such as intravenous infusion, oral preparations, and intraperitoneal and intravenous administration. We prefer that infusion times of up to 24 hours are used, more preferably 1-12 hours, with 1-6 hours most preferred. Short infusion times which allow treatment to be carried out without an overnight stay in hospital are especially desirable. However, infusion may be 12 to 24 hours or even longer if required. Infusion may be carried out at suitable intervals of say 1 to 4 weeks. Pharmaceutical compositions containing compounds of the invention may be delivered by liposome or nanosphere encapsulation, in sustained release formulations or by other standard delivery means. 
         [0067]    The correct dosage of the compounds will vary according to the particular formulation, the mode of application, and the particular situs, host and tumour being treated. Other factors like age, body weight, sex, diet, time of administration, rate of excretion, condition of the host, drug combinations, reaction sensitivities and severity of the disease shall be taken into account. Administration can be carried out continuously or periodically within the maximum tolerated dose. 
         [0068]    As used herein, the terms “treat”, “treating” and “treatment” include the eradication, removal, modification, or control of a tumor or primary, regional, or metastatic cancer cells or tissue and the minimization or delay of the spread of cancer. 
         [0069]    The compounds of the invention have activity against cancers including, but not limited, lung cancer, colon cancer, and breast cancer. 
       EXAMPLES 
     Example 1 
     Description of the Marine Organism and Collection Site 
       [0070]      Mycale  ( Aegogropila )  crassissima  (Dendy, 1905) was collected by hand using SCUBA diving in Mafia Island (07° 39.558′ S/39° 55.043′ E) at depths ranging between 5 and 31.4 m. The animal material was identified by Dr. José Luis Carballo (Universidad Nacional Autónoma of Mexico). A sample of the specimen was deposited at the Institute of Marine Sciences and Limnology of the Universidad Nacional Autónoma of Mexico, with the reference code MFIA-399. 
       Example 2 
     Isolation of Aegomycins A and B 
       [0071]    The frozen specimen of Example 1 (67 g) was triturated and extracted with H 2 O and a mixture of MeOH:CH 2 Cl 2  (50:50) at 23° C. The organic extract was evaporated under reduced pressure to yield a crude of 500 mg. This material was chromatographed (VLC) on Lichroprep RP-18 with a stepped gradient from H 2 O to MeOH and CH 2 Cl 2 . Two fractions obtained from this chromatography were further purified as described below. The fraction eluted with H 2 O:MeOH 1:3 (23 mg) was subjected to semipreparative reversed phase HPLC (Symmetry Prep C18, 7.8×150 mm, gradient H 2 O:MeCN from 60 to 75% of MeCN in 10 min then 75 to 100% of MeCN in 10 min, UV detection, flow 2.3 mL/min) to yield Aegomycin A (0.6 mg). The fraction eluted with MeOH (83 mg) was subjected to semipreparative reversed phase HPLC (Symmetry Prep C18, 7.8×150 mm, gradient H 2 O:MeCN from 50 to 75% of MeCN in 30 min, UV detection, flow 2.3 mL/min) to yield Aegomycin A (1.8 mg) and Aegomycin B (0.8 mg). 
         [0072]    Aegomycin A: amorphous white solid. (+)HRMALDIMS m/z 523.1622 [M+H] +  (Calcd. for C 27 H 33   35 Cl 2 O 6  523.1649);  1 H (500 MHz) and  13 C NMR (75 MHz) in CDCl 3  see Table 1 
         [0073]    Aegomycin B: amorphous white solid. (+)HRMALDIMS m/z 525.1788 [M+H] +  (Calcd. for C 27 H 35   35 Cl 2 O 6  525.1805);  1 H (500 MHz) and  13 C NMR (75 MHz) in CD 3 OD see Table 2. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin A (CDCl 3 ). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 3.74, d (3.8) 
                 54.3, d 
                 C5, C9, 
                 H2, H11a 
               
               
                   
                   
                   
                 C10, C19 
                   
               
               
                  2 
                 3.25, d (3.8) 
                 55.7, d 
                 C3, C4 
                 OMe, H1, H3 
               
               
                  3 
                 2.88, s 
                 84.4, d 
                 C1, C2, C4, 
                 OMe, H2, 
               
               
                   
                   
                   
                 C20, C21, OMe 
                 H5, H20 
               
               
                  4 
                 — 
                 36.7, s 
                 — 
                 — 
               
               
                  5 
                 1.51, dd 
                 41.0, d 
                 C4, C9, C10, 
                 H3, H6, H9, H20 
               
               
                   
                 (10.6, 7.8) 
                   
                 C6, C19, C21 
                   
               
               
                  6 
                 2.40, m 
                 22.7, t 
                 C7 
                 H5, H7, H19, 
               
               
                   
                   
                   
                   
                 H20, H21 
               
               
                  7 
                 6.19, m 
                 122.8, d 
                 — 
                 H6, 14-OH 
               
               
                  8 
                 — 
                 137.5, s 
                 — 
                 — 
               
               
                  9 
                 2.62, m 
                 41.1, d 
                 — 
                 H5, H11a, 
               
               
                   
                   
                   
                   
                 H12a, H15a 
               
               
                 10 
                 — 
                 49.8, s 
                 — 
                 — 
               
               
                  11a 
                 1.90, m 
                 22.7, t 
                 C8 
                 H1, H9, H11b 
               
               
                  11b 
                 1.25, m 
                   
                 C9 
                 H11a, H12b, 
               
               
                   
                   
                   
                   
                 H18, H19 
               
               
                  12a 
                 1.89, m 
                 39.3, t 
                 C13, C14, 
                 H9, H12b, 
               
               
                   
                   
                   
                 C15, C18 
                 H15a, H17 
               
               
                  12b 
                 1.72, m 
                   
                 C13 
                 H11b, H12a, 
               
               
                   
                   
                   
                   
                 H17, H18 
               
               
                 13 
                 — 
                 51.0, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 83.8, s 
                 — 
                 — 
               
               
                 14-OH 
                 1.87, m 
                 — 
                 C14 
                 H7, H18 
               
               
                  15a 
                 3.47, d (15.8) 
                 62.0 t 
                 C13, C14, C16 
                 H9, H12a, H15b 
               
               
                  15b 
                 2.81, d (15.8) 
                   
                 C13, C14, 
                 H15a 
               
               
                   
                   
                   
                 C16, C17 
                   
               
               
                 16 
                 — 
                 92.5, s 
                 — 
                 — 
               
               
                 17 
                 3.46, s 
                 71.2, d 
                 C12, C13, C16, 
                 H12a, H12b, H23 
               
               
                   
                   
                   
                 C22, C23, C24 
                   
               
               
                 18 
                 0.74, s 
                 16.5, q 
                 C12, C13, 
                 H11b, H12b, 
               
               
                   
                   
                   
                 C14, C17 
                 H24, 14-OH 
               
               
                 19 
                 9.87, s 
                 205.6, d 
                 C1, C10 
                 H6, H11b, H21 
               
               
                 20 
                 0.94, s 
                 25.0, q 
                 C3, C4, C5, C21 
                 OMe, H3, H5, 
               
               
                   
                   
                   
                   
                 H6, H21 
               
               
                 21 
                 0.66, s 
                 16.2, q 
                 C3, C4, C5, C20 
                 H6, H19, H20 
               
               
                 22 
                 — 
                 116.7, s 
                 — 
                 — 
               
               
                 23 
                 7.33, d (2.7) 
                 151.0, d 
                 C17, C22, C24 
                 H17 
               
               
                 24 
                 8.00, dd (9.9, 2.7) 
                 147.9, d 
                 C26 
                 H18, H25 
               
               
                 25 
                 6.28, d (9.9) 
                 113.9, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 161.4, s 
                 — 
                 — 
               
               
                 OMe 
                 3.51, s 
                 58.3, q 
                 C3 
                 H2, H3, H20 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin B (CD 3 OD). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 3.74, d (3.7) 
                 56.6, d 
                 C5, C10 
                 H2, H11 
               
               
                  2 
                 3.18, d (3.7) 
                 57.5, d 
                 C3, C4 
                 OMe, H1, H3 
               
               
                  3 
                 2.85, s 
                 87.0, d 
                 OMe, C1, C2, 
                 OMe, H2, H5, 
               
               
                   
                   
                   
                 C4, C5, C20, C21 
                 H20 
               
               
                  4 
                 — 
                 37.6, s 
                 — 
                 — 
               
               
                  5 
                 1.05, dd 
                 43.2, d 
                 C3, C4, C6, C9, 
                 H3, H9, H20 
               
               
                   
                 (12.4, 5.0) 
                   
                 C10, C19, C21 
                   
               
               
                  6 
                 1.99, m 
                 22.7, t 
                 C7, C8 
                 H7, H20 
               
               
                  7 
                 6.05, m 
                 124.6, d 
                 — 
                 H6 
               
               
                  8 
                 — 
                 139.6, s 
                 — 
                 — 
               
               
                  9 
                 2.42, m 
                 46.1, d 
                 — 
                 H5, H15a 
               
               
                 10 
                 — 
                 40.0, s 
                 — 
                   
               
               
                 11 
                 1.90, m 
                 23.5, t 
                 C12 
                 H1, H18 
               
               
                 12 
                 1.79, m 
                 40.7, t 
                 C11 
                 H17, H18 
               
               
                 13 
                 — 
                 52.6, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 84.7, s 
                 — 
                 — 
               
               
                  15a 
                 3.43, d (15.8) 
                 64.1, t 
                 C8, C13, C14, C16 
                 H9, H15b 
               
               
                  15b 
                 2.76, d (15.8) 
                   
                 C14, C16, C17 
                 H15a 
               
               
                 16 
                 — 
                 94.9, s 
                 — 
                 — 
               
               
                 17 
                 3.61, s 
                 72.8, d 
                 C12, C13, C14, C16, 
                 H12, H23 
               
               
                   
                   
                   
                 C22, C24, C25 
                   
               
               
                 18 
                 0.79, s 
                 17.3, q 
                 C12, C13, C14, C17 
                 H11, H12, 
               
               
                   
                   
                   
                   
                 H24 
               
               
                  19a 
                 3.98, d (12.1) 
                 60.7, t 
                 C1, C5, C9, C10 
                 H19b, H21 
               
               
                  19b 
                 3.75, d (12.1) 
                   
                 C1, C5, C9, C10 
                 H19a 
               
               
                 20 
                 0.89, s 
                 26.4, q 
                 C3, C4, C5, C21 
                 H3, H5, H6 
               
               
                 21 
                 0.92, s 
                 17.3, q 
                 C3, C4, C5, C20 
                 H19a 
               
               
                 22 
                 — 
                 119.8, s 
                 — 
                 — 
               
               
                 23 
                 7.57, d (2.2) 
                 152.9, d 
                 C17, C22, C24, C26 
                 H17 
               
               
                 24 
                 8.30, dd (9.8, 2.2) 
                 151.5, d 
                 C23, C26 
                 H18, H25 
               
               
                 25 
                 6.28, d (9.8) 
                 113.7, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 164.1, s 
                 — 
                 — 
               
               
                 OMe 
                 3.49, s 
                 58.4, q 
                 C3 
                 H2, H3 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
       Example 3 
     Isolation of Aegomycins C, D, E, F and G 
       [0074]    A second group of samples of the specimen of Example 1 (160 g) was triturated and extracted with H 2 O and a mixture of MeOH:CH 2 Cl 2  (50:50) at 23° C. The organic extract was evaporated under reduced pressure to yield a crude of 3.12 g. This material was chromatographed (VLC) on Lichroprep RP-18 with a stepped gradient from H 2 O to MeOH and CH 2 Cl 2 . Two fractions obtained from this chromatography were further purified as described below. The fraction eluted with H 2 O:MeOH 1:3 (122 mg) was subjected to semipreparative reversed phase HPLC (Symmetry Prep C18, 7.8×150 mm, gradient H 2 O:MeCN from 45 to 65% of MeCN in 30 min, UV detection, flow 2.3 mL/min) to yield Aegomycin F (2.6 mg) and a mixture of other Aegomycin compounds (5.1 mg). This mixture was further purified by semipreparative HPLC(X Terra Phenyl, 10×150 mm, gradient H 2 O:MeCN from 45 to 60% of MeCN in 30 min, UV detection, flow 2.3 mL/min) to yield Aegomycin C (0.5 mg) and D (2.8 mg). The fraction eluted with MeOH (230 mg) was subjected to flash Silica gel CC eluting with a gradient of hexane:EtOAc to yield 11 fractions (S1 to S11). Fractions S6 (hexane:EtOAc 70:30) and S7 (hexane:EtOAc 60:40) were subjected to semipreparative reversed phase HPLC(X Terra Phenyl, 10×150 mm, gradient H 2 O:MeCN from 50 to 55% of MeCN in 30 min, UV detection, flow 3.0 mL/min) to yield Aegomycin E (1.3 mg) from S6 and Aegomycin G (0.3 mg) from S7. 
         [0075]    Aegomycin C: amorphous white solid. (+)HRMALDIMS m/z 477.1581 [M+H] +  (Calcd. for C 26 H 31   35 Cl 2 O 4  477.1594);  1 H (500 MHz) and  13 C NMR (75 MHz) in CD 3 OD see Table 3. 
         [0076]    Aegomycin D: amorphous white solid. (+)HRMALDIMS m/z 509.1834 [M+H] +  (Calcd. for C 27 H 35   35 Cl 2 O 5  509.1856);  1 H (500 MHz) and  13 C NMR (125 MHz) in CD 3 OD see Table 4. 
         [0077]    Aegomycin E: amorphous white solid. (+)HRMALDIMS m/z 493.1898 [M+H]+(Calcd. for C 27 H 35   35 Cl 2 O 4  493.1907);  1 H (500 MHz) and  13 C NMR (75 MHz) in CD 3 OD see Table 5. 
         [0078]    Aegomycin F: amorphous white solid. (+)HRMALDIMS m/z 443.1997 [M+H] +  (Calcd. for C 26 H 32   35 ClO 4  443.1984);  1 H (500 MHz) and  13 C NMR (75 MHz) in CD 3 OD see Table 6. 
         [0079]    Aegomycin G: amorphous white solid. (+)HRMALDIMS m/z 459.2306 [M+H] +  (Calcd. for C 27 H 36   35 ClO 4  459.2297);  1 H (500 MHz) and  13 C NMR (75 MHz) in CD 3 OD see Table 7. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin C (CD 3 OD). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 7.12, d (10.3) 
                 157.2, d 
                 C3, C5, C10 
                 H2, H11a 
               
               
                  2 
                 5.91, d (10.3) 
                 124.4, d 
                 C4, C10 
                 H1 
               
               
                  3 
                 — 
                 206.7, s 
                 — 
                 — 
               
               
                  4 
                 — 
                 44.9, s 
                 — 
                 — 
               
               
                  5 
                 1.85, m 
                 48.9, d 
                 C4, C6, C10, 
                 H6, H9, H20 
               
               
                   
                   
                   
                 C19 
                   
               
               
                  6 
                 2.17, m 
                 24.0, t 
                 — 
                 H5, H7, H9, H20 
               
               
                  7 
                 6.15, m 
                 124.4, d 
                 C5, C14 
                 H6 
               
               
                  8 
                 — 
                 138.4, s 
                 — 
                 — 
               
               
                  9 
                 2.21, m 
                 46.9, d 
                 — 
                 H5, H6, H15a 
               
               
                 10 
                 — 
                 38.9, s 
                 — 
                 — 
               
               
                  11a 
                 1.87, m 
                 21.9, t 
                 C19 
                 H1, H11b 
               
               
                  11b 
                 1.54, dddd (13.7, 
                   
                 — 
                 H11a, H12b, 
               
               
                   
                 13.7, 13.7, 3.4) 
                   
                   
                 H18, H19 
               
               
                  12a 
                 1.96, ddd (13.7, 
                 40.3, t 
                 — 
                 H12b, H15a, 
               
               
                   
                 13.7, 3.4) 
                   
                   
                 H17 
               
               
                  12b 
                 1.79, ddd (13.7, 
                   
                 C18 
                 H11b, H12a, 
               
               
                   
                 3.4, 3.4) 
                   
                   
                 H17, H18 
               
               
                 13 
                 — 
                 52.4, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 84.7, s 
                 — 
                 — 
               
               
                  15a 
                 3.44, d (16.1) 
                 63.6, t 
                 C8, C13, C14, C16 
                 H9, H12a, H15b 
               
               
                  15b 
                 2.78, d (16.1) 
                   
                 C14, C16, C17 
                 H15a 
               
               
                 16 
                 — 
                 94.9, s 
                 — 
                 — 
               
               
                 17 
                 3.62, s 
                 72.5, d 
                 C12, C13, C14, 
                 H12a, H12b, 
               
               
                   
                   
                   
                 C16, C22, C23 
                 H23 
               
               
                 18 
                 0.77, s 
                 17.2, q 
                 C12, C13, C14, C17 
                 H11b, H12b 
               
               
                 19 
                 1.07, s 
                 14.9, q 
                 C1, C5, C9, C10 
                 H11b, H21 
               
               
                 20 
                 1.15, s 
                 24.0, q 
                 C3, C4, C5, C21 
                 H5, H6 
               
               
                 21 
                 1.13, s 
                 22.5, q 
                 C3, C4, C5, C20 
                 H19 
               
               
                 22 
                 — 
                 119.7, s 
                 — 
                 — 
               
               
                 23 
                 7.58, d (2.2) 
                 152.9, d 
                 C22, C24, C26 
                 H17 
               
               
                 24 
                 8.32, dd 
                 151.4, d 
                 C23, C26 
                 H25 
               
               
                   
                 (10.3, 2.2) 
                   
                   
                   
               
               
                 25 
                 6.29, d (10.3) 
                 113.7, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 164.1, s 
                 — 
                 — 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin D (CD 3 OD). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 5.82, dd (10.7, 2.4) 
                 135.3, d 
                 C3, C5 
                 H2, H11a, H19 
               
               
                  2 
                 5.67, dd (10.7, 1.0) 
                 126.5, d 
                 — 
                 H1, OMe 
               
               
                  3 
                 3.40, m 
                 87.3, d 
                 OMe, C1, C2, 
                 H5, H20 
               
               
                   
                   
                   
                 C4, C20, C21 
                   
               
               
                  4 
                 — 
                 37.7, s 
                 — 
                 — 
               
               
                  5 
                 1.45, dd (11.9, 4.2) 
                 49.2, d 
                 C3, C4, C6, C9, 
                 H3, H9, H20 
               
               
                   
                   
                   
                 C10, C19, C21 
                   
               
               
                  6a 
                 2.14, m 
                 23.4, t 
                 C7, C8 
                 H6b, H7, H20 
               
               
                  6b 
                 2.04, m 
                   
                 — 
                 H6a, H19, H21 
               
               
                  7 
                 6.12, m 
                 125.9, d 
                 C5, C9, C14 
                 H6a 
               
               
                  8 
                 — 
                 137.9, s 
                 — 
                 — 
               
               
                  9 
                 2.08, m 
                 46.8, d 
                 C8, C10 
                 H5, H11a, 
               
               
                   
                   
                   
                   
                 H12, H15a 
               
               
                 10 
                 — 
                 38.4, s 
                 — 
                   
               
               
                  11a 
                 1.83, ddd (12.7, 
                 30.3, t 
                 C8, C9, 
                 H1, H9, H12, 
               
               
                   
                 4.4, 4.2) 
                   
                 C12, C13 
                 H11b 
               
               
                  11b 
                 1.53, ddd (12.7, 
                   
                 C9, C10, 
                 H11a, H18, H19 
               
               
                   
                 12.7, 11.9) 
                   
                 C12, C13 
                   
               
               
                 12 
                 3.84, dd (11.9, 4.2) 
                 74.9, d 
                 C17, C18 
                 H9, H11a, H15a, 
               
               
                   
                   
                   
                   
                 H17 
               
               
                 13 
                 — 
                 58.3, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 85.0, s 
                 — 
                 — 
               
               
                  15a 
                 3.28, d (16.1) 
                 64.0, t 
                 C8, C14, C16 
                 H9, H12, H15b 
               
               
                  15b 
                 2.80, d (16.1) 
                   
                 C13, C14, 
                 H15a 
               
               
                   
                   
                   
                 C16, C17 
                   
               
               
                 16 
                 — 
                 95.1, s 
                 — 
                 — 
               
               
                 17 
                 4.15, s 
                 68.2, d 
                 C12, C13, 
                 H12, H18, H23 
               
               
                   
                   
                   
                 C14, C16, C22, 
                   
               
               
                   
                   
                   
                 C23, C24 
                   
               
               
                 18 
                 0.65, s 
                 10.5, q 
                 C12, C13, 
                 H11b, H17, 
               
               
                   
                   
                   
                 C14, C17 
                 H19, H24 
               
               
                 19 
                 0.92, s 
                 15.3, q 
                 C1, C5, C9, C10 
                 H1, H6b, H11b, 
               
               
                   
                   
                   
                   
                 H18, H21 
               
               
                 20 
                 0.99, s 
                 26.6, q 
                 C3, C4, C5, C21 
                 H3, H5, H6a, H21 
               
               
                 21 
                 0.85, s 
                 17.2, q 
                 C3, C4, C5, C20 
                 H6b, H19, H20 
               
               
                 22 
                 — 
                 120.0, s 
                 — 
                 — 
               
               
                 23 
                 7.54, d (2.9) 
                 156.0, d 
                 C22, C24, C26 
                 H17 
               
               
                 24 
                 8.27, dd (9.8, 2.9) 
                 151.6, d 
                 C23, C26 
                 H18, H25 
               
               
                 25 
                 6.28, d (9.8) 
                 113.7, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 164.1, s 
                 — 
                 — 
               
               
                 OMe 
                 3.41, s 
                 58.3, q 
                 C3 
                 H2 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin E (CD 3 OD). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 5.83, dd (10.5, 2.3) 
                 135.7, d 
                 C2, C5, C9 
                 H2, H11a, H19 
               
               
                  2 
                 5.65, dd (10.5, 1.5) 
                 126.2, d 
                 C4 
                 H1, H3 
               
               
                  3 
                 3.40, m 
                 87.3, d 
                 C1 
                 H2, H5, H20 
               
               
                  4 
                 — 
                 37.7, s 
                 — 
                 — 
               
               
                  5 
                 1.42, dd (12.0, 4.5) 
                 48.9, d 
                 C19 
                 H3, H9, H20 
               
               
                  6a 
                 2.12, m 
                 23.4, t 
                 — 
                 H6b, H7, H20 
               
               
                  6b 
                 2.01, m 
                   
                   
                 H6a, H19, H21 
               
               
                  7 
                 6.11, br d (5.6) 
                 125.2, d 
                 C5, C14 
                 H6a 
               
               
                  8 
                 — 
                 139.0, s 
                 — 
                 — 
               
               
                  9 
                 2.02, m 
                 49.1, d 
                 — 
                 H5, H15a 
               
               
                 10 
                 — 
                 38.6, s 
                 — 
                 — 
               
               
                  11a 
                 1.71, m 
                 21.9, t 
                 — 
                 H1, H11b 
               
               
                  11b 
                 1.51, dddd (13.8, 
                   
                   
                 H11a, H18, H19 
               
               
                   
                 13.7, 13.7, 3.4) 
                   
                   
                   
               
               
                  12a 
                 1.90, ddd 
                 40.5, t 
                 — 
                 H12b, H15a, H17 
               
               
                   
                 (13.7, 13.7, 3.4) 
                   
                   
                   
               
               
                  12b 
                 1.74, m 
                   
                   
                 H12a, H17 
               
               
                 13 
                 — 
                 52.5, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 84.8, s 
                 — 
                 — 
               
               
                  15a 
                 3.43, d (15.6) 
                 63.9, t 
                 C16 
                 H9, H12a, H15b 
               
               
                  15b 
                 2.76, d (15.6) 
                   
                 C14, C16, C17 
                 H15a 
               
               
                 16 
                 — 
                 95.0, s 
                 — 
                 — 
               
               
                 17 
                 3.59, s 
                 72.6, d 
                 C14, C16, C22, 
                 H12a, H12b, H23 
               
               
                   
                   
                   
                 C23, C24 
                   
               
               
                 18 
                 0.74, s 
                 17.2, q 
                 C12, C13, 
                 H11b, H24 
               
               
                   
                   
                   
                 C14, C17 
                   
               
               
                 19 
                 0.91, s 
                 15.3, q 
                 C1, C5, C9, C10 
                 H1, H6b, 
               
               
                   
                   
                   
                   
                 H11b, H21 
               
               
                 20 
                 0.99, s 
                 26.7, q 
                 C3, C4, C5, C21 
                 H3, H5, H6a, H21 
               
               
                 21 
                 0.85, s 
                 17.2, q 
                 C3, C4, C5, C20 
                 H6b, H19, H20 
               
               
                 22 
                 — 
                 119.7, s 
                 — 
                 — 
               
               
                 23 
                 7.57, d (2.4) 
                 152.8, d 
                 C22 
                 H17 
               
               
                 24 
                 8.31, dd (9.8, 2.4) 
                 151.5, d 
                 — 
                 H18, H25 
               
               
                 25 
                 6.28, d (9.8) 
                 113.7, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 164.1, s 
                 — 
                 — 
               
               
                 OMe 
                 3.41, s 
                 58.3, q 
                 C3 
                 — 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin F (CD 3 OD). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 7.10, d (10.3) 
                 157.5, d 
                 C3, C5, C6, 
                 H2, H11a, H19 
               
               
                   
                   
                   
                 C9, C10, C19 
                   
               
               
                  2 
                 5.88, d (10.3) 
                 126.1, d 
                 C4, C10, C19 
                 H1 
               
               
                  3 
                 — 
                 206.8, s 
                 — 
                 — 
               
               
                  4 
                 — 
                 45.0, s 
                 — 
                 — 
               
               
                  5 
                 1.83, dd (12.1, 4.2) 
                 48.5, d 
                 C4, C6, C9, 
                 H6, H9, H20 
               
               
                   
                   
                   
                 C19, C20, C21 
                   
               
               
                  6 
                 2.18, m 
                 23.9, t 
                 C7, C8 
                 H5, H7, H19, 
               
               
                   
                   
                   
                   
                 H20, H21 
               
               
                  7 
                 6.13, br d (5.0) 
                 123.0, d 
                 C5, C6, C14 
                 H6 
               
               
                  8 
                 — 
                 140.0, s 
                 — 
                 — 
               
               
                  9 
                 2.14, m 
                 47.3, d 
                 C7, C8, C10 
                 H5, H11a, 
               
               
                   
                   
                   
                   
                 H12b, H15a 
               
               
                 10 
                 — 
                 38.5, s 
                 — 
                 — 
               
               
                  11a 
                 1.79, m 
                 21.8, t 
                 C8, C9, C12, C13 
                 H1, H9, H11b 
               
               
                  11b 
                 1.48, dddd (12.6, 
                   
                 C9, C12 
                 H11a, H18, H19 
               
               
                   
                 12.6, 12.6, 3.9) 
                   
                   
                   
               
               
                  12a 
                 1.65, ddd (13.7, 
                 39.5, t 
                 C9, C11, C13, 
                 H12b, H17, H18 
               
               
                   
                 3.9, 3.9) 
                   
                 C14, C18 
                   
               
               
                  12b 
                 1.59, ddd (13.7, 
                   
                 C11, C13 
                 H9, H12a, H17 
               
               
                   
                 12.6, 3.0) 
                   
                   
                   
               
               
                 13 
                 — 
                 52.0, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 84.7, s 
                 — 
                 — 
               
               
                  15a 
                 3.00, dd (16.0, 9.5) 
                 52.2, t 
                 C8, C16 
                 H9, H15b, H16 
               
               
                  15b 
                 2.01, dd (16.0, 2.9) 
                   
                 C13, C14, C16, 
                 H15a 
               
               
                   
                   
                   
                 C17 
                   
               
               
                 16 
                 4.90, ddd (9.5, 
                 60.3, d 
                 C14, C22 
                 H15a, H17 
               
               
                   
                 9.5, 2.9) 
                   
                   
                   
               
               
                 17 
                 3.12, d (9.5) 
                 58.9, d 
                 C12, C13, C14, 
                 H12a, H12b, 
               
               
                   
                   
                   
                 C15, C16, C23, 
                 H16, H18, H23 
               
               
                   
                   
                   
                 C24 
                   
               
               
                 18 
                 0.76, s 
                 17.6, q 
                 C12, C13, C14, 
                 H11b, H12a, 
               
               
                   
                   
                   
                 C17 
                 H17, H23, H24 
               
               
                 19 
                 1.05, s 
                 14.9, q 
                 C1, C5, C9, C10 
                 H1, H6, H11b, 
               
               
                   
                   
                   
                   
                 H21 
               
               
                 20 
                 1.13, s 
                 25.5, q 
                 C3, C4, C5, C21 
                 H5, H6 
               
               
                 21 
                 1.11, s 
                 22.5, q 
                 C3, C4, C5, C20 
                 H6, H19 
               
               
                 22 
                 — 
                 121.2, s 
                 — 
                 — 
               
               
                 23 
                 7.46, d (2.3) 
                 152.4, d 
                 C17, C22, C24, 
                 H17, H18 
               
               
                   
                   
                   
                 C26 
                   
               
               
                 24 
                 8.36, dd (9.8, 2.3) 
                 153.3, d 
                 C23, C26 
                 H18, H25 
               
               
                 25 
                 6.22, d (9.8) 
                 112.8, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 164.6, s 
                 — 
                 — 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin G (CD 3 OD). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 5.81, br d (10.4) 
                 136.0, d 
                 C3 
                 H2, H11a, H19 
               
               
                  2 
                 5.62, d (10.4) 
                 120.0, d 
                 C4, C10 
                 H1, H3, OMe 
               
               
                  3 
                 3.67, br s 
                 87.4, d 
                 OMe, C1, C2, 
                 H2, H5, H20 
               
               
                   
                   
                   
                 C4, C20, C21 
                   
               
               
                  4 
                 — 
                 37.7, s 
                 — 
                 — 
               
               
                  5 
                 1.40, dd 
                 49.4, d 
                 — 
                 H3, H6a, 
               
               
                   
                 (12.0, 4.3) 
                   
                   
                 H9, H20 
               
               
                  6a 
                 2.07, m 
                 23.7, t 
                 — 
                 H5, H7, H20 
               
               
                  6b 
                 1.99, m 
                   
                   
                 H7, H21 
               
               
                  7 
                 6.08, br d (5.4) 
                 123.8, d 
                 C14 
                 H6a, H6b 
               
               
                  8 
                 — 
                 140.6, s 
                 — 
                 — 
               
               
                  9 
                 1.99, m 
                 49.0, d 
                 — 
                 H5, H15a 
               
               
                 10 
                 — 
                 38.6, s 
                 — 
                 — 
               
               
                  11a 
                 1.62, m 
                 21.8, t 
                 — 
                 H1, H11b 
               
               
                  11b 
                 1.45, dddd (12.6, 
                   
                   
                 H11a, H18, 
               
               
                   
                 12.6, 12.6, 4.1) 
                   
                   
                 H19 
               
               
                  12a 
                 1.58, m 
                 39.7, t 
                 — 
                 H17 
               
               
                  12b 
                 1.54, m 
                   
                   
                 H15a, H17 
               
               
                 13 
                 — 
                 52.0, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 85.0, s 
                 — 
                 — 
               
               
                  15a 
                 2.97, dd 
                 52.3, t 
                 C17 
                 H9, H12b, 
               
               
                   
                 (15.7, 9.6) 
                   
                   
                 H15b, H16 
               
               
                  15b 
                 2.00, dd (15.7, 
                   
                 — 
                 H15a 
               
               
                   
                 3.0) 
                   
                   
                   
               
               
                 16 
                 4.89, ddd (9.6, 
                 60.3, d 
                 — 
                 H15a, H17 
               
               
                   
                 9.5, 3.0) 
                   
                   
                   
               
               
                 17 
                 3.09, d, (9.5) 
                 59.0, d 
                 C12, C13, C14, 
                 H12a, H12b, 
               
               
                   
                   
                   
                 C22, C24 
                 H16, H23 
               
               
                 18 
                 0.73, s 
                 17.6, q 
                 C12, C13 C14,C17 
                 H11b, H24 
               
               
                 19 
                 0.90, s 
                 15.4, q 
                 C1, C5, C9, C10 
                 H1, H11b 
               
               
                 20 
                 0.97, s 
                 26.7, q 
                 C4, C5, C3, C21 
                 H3, H5, H6a 
               
               
                 21 
                 0.83, s 
                 17.2, q 
                 C4, C5, C3, C20 
                 H6b 
               
               
                 22 
                 — 
                 121.3, s 
                 — 
                 — 
               
               
                 23 
                 7.45, d (2.5) 
                 152.3, d* 
                 C22, C24, C26 
                 H17 
               
               
                 24 
                 8.36, dd (9.9, 2.5) 
                 152.3, d* 
                 — 
                 H18, H25 
               
               
                 25 
                 6.21, d (9.9) 
                 112.7, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 164.7, s 
                 — 
                 — 
               
               
                 OMe 
                 3.39, s 
                 58.3, q 
                 C3 
                 H2 
               
               
                   
               
               
                 *Assignments may be interchanged 
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
       Example 4 
     Isolation of Aegomycin D, F and H 
       [0080]    A third group of samples of the specimen of Example 1 (700 g) was triturated and extracted with H 2 O and a mixture of MeOH:CH 2 Cl 2  (50:50) at 23° C. The organic extract was evaporated under reduced pressure to yield a crude of 33 g. The crude was dissolved in MeOH:H 2 O (1:9, 500 mL) and extracted with Hexane (3×500 mL), EtOAc (3×500 mL) and nBuOH (2×500 mL). 
         [0081]    The Hexane fraction (4 g) was chromatographed (VLC) on Lichroprep RP-18 with a stepped gradient from H 2 O:MeOH (3:1) to MeOH and then to CH 2 Cl 2 . The fraction eluted with H 2 O:MeOH 1:3 (430 mg) was subjected to preparative reversed phase HPLC (Symmetry Prep C18, 19×150 mm, gradient H 2 O:MeCN from 45 to 65% of MeCN in 30 min, UV detection, flow 14.6 mL/min) to yield 7 fractions (H1 to H7). Fraction H4 was subjected to semipreparative reversed phase HPLC(X Terra Phenyl, 10×150 mm, gradient H 2 O:MeCN from 30 to 50% of MeCN in 30 min, UV detection, flow 3.8 mL/min) to yield Aegomycin F (8.3 mg). Fraction H5 was subjected to semipreparative reversed phase HPLC(X Terra Phenyl, 10×150 mm, gradient H 2 O:MeCN from 45 to 60% of MeCN in 30 min, UV detection, flow 3.8 mL/min) to yield Aegomycin D (20.4 mg). 
         [0082]    The EtOAc fraction (1.2 g) was chromatographed (VLC) on Lichroprep RP-18 with a stepped gradient from H 2 O:MeOH (3:1) to MeOH and then to CH 2 Cl 2 . The fraction eluted with H 2 O:MeOH 1:3 (162 mg) was subjected to preparative reversed phase HPLC (Symmetry Prep C18, 19×150 mm, gradient H 2 O:MeCN from 45 to 65% of MeCN in 30 min, UV detection, flow 14.6 mL/min) to yield 7 fractions (H1 to H7). Fraction H2 was subjected to semipreparative reversed phase HPLC(X Terra Phenyl, 10×150 mm, gradient H 2 O:MeCN from 30 to 55% of MeCN in 30 min, UV detection, flow 3.8 mL/min) to yield Aegomycin H (1.4 mg). Fraction H4 was subjected to semipreparative reversed phase HPLC(X Terra Phenyl, 10×150 mm, gradient H 2 O:MeCN from 30 to 50% of MeCN in 20 min, UV detection, flow 3.8 mL/min) to yield Aegomycin F (12.2 mg). Fraction H5 was subjected to semipreparative reversed phase HPLC(X Terra Phenyl, 10×150 mm, gradient H 2 O:MeCN from 45 to 60% of MeCN in 30 min, UV detection, flow 3.8 mL/min) to yield Aegomycin D (21.7 mg). 
         [0083]    Aegomycin H: amorphous white solid. (+)ESIMS m/z 493 [M+H] + ,  1 H (500 MHz) and  13 C NMR (75 MHz) in CD 3 OD see Table 8. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                   1 H and  13 C NMR data of Aegomycin H (CD 3 OD). 
               
             
          
           
               
                 N o   
                   1 H, m, J (Hz) 
                   13 C, m 
                 HMBC 
                 ROESY 
               
               
                   
               
               
                  1 
                 7.10, d (10.3) 
                 156.7, d 
                 C3, C4, C5, 
                 H2, H9, 
               
               
                   
                   
                   
                 C9, C10, C19 
                 H11a, H19 
               
               
                  2 
                 5.91, d (10.3) 
                 126.4, d 
                 C4, C10 
                 H1 
               
               
                  3 
                 — 
                 206.5, s 
                 — 
                 — 
               
               
                  4 
                 — 
                 45.0, s 
                 — 
                 — 
               
               
                  5 
                 1.89, dd (11.7, 
                 48.5, d 
                 C4, C6, C7, 
                 H9, H20 
               
               
                   
                 4.5) 
                   
                 C9, C10, C19 
                   
               
               
                  6 
                 2.19, m 
                 24.0, t 
                 C8 
                 H7, H19, 
               
               
                   
                   
                   
                   
                 H20, H21 
               
               
                  7 
                 6.15, m 
                 125.1, d 
                 C5, C14 
                 H6 
               
               
                  8 
                 — 
                 137.3, s 
                 — 
                 — 
               
               
                  9 
                 2.27, m 
                 44.6, d 
                   
                 H1, H5, H11a, 
               
               
                   
                   
                   
                   
                 H12, H15a 
               
               
                 10 
                 — 
                 38.6, s 
                 — 
                 — 
               
               
                  11a 
                 1.95, ddd (12.6, 
                 30.2, t 
                 C8, C9, C12, C13 
                 H1, H9, H11b, 
               
               
                   
                 4.2, 4.2) 
                   
                   
                 H12 
               
               
                  11b 
                 1.55, ddd (12.6,  
                   
                 C8, C9, C10, C12, 
                 H11a, H18, 
               
               
                   
                 12.6, 12.6) 
                   
                 C13 
                 H19 
               
               
                 12 
                 3.89, dd 
                 74.7, d 
                 C17, C18 
                 H9, H11a, 
               
               
                   
                 (12.6, 4.2) 
                   
                   
                 H15a, H17 
               
               
                 13 
                 — 
                 58.2, s 
                 — 
                 — 
               
               
                 14 
                 — 
                 84.9. s 
                 — 
                 — 
               
               
                  15a 
                 3.33, d (15.9) 
                 63.7, t 
                 C8, C14, C16 
                 H9, H12, H15b 
               
               
                  15b 
                 2.80, d (15.9) 
                   
                 C13, C14, C16, C17 
                 H15a 
               
               
                 16 
                 — 
                 95.0, s 
                 — 
                 — 
               
               
                 17 
                 4.15, s 
                 68.2, d 
                 C12, C13, C14, C16, 
                 H12, H18, H23 
               
               
                   
                   
                   
                 C22, C23, C24 
                   
               
               
                 18 
                 0.67, s 
                 10.4, q 
                 C12, C13, C14, C17 
                 H11b, H17, 
               
               
                   
                   
                   
                   
                 H23, H24 
               
               
                 19 
                 1.07, s 
                 14.9, q 
                 C1, C5, C9, C10 
                 H1, H6, H11b 
               
               
                 20 
                 1.14, s 
                 25.4, q 
                 C4, C5, C21 
                 H5, H6 
               
               
                 21 
                 1.12, s 
                 22.5, q 
                 C4, C5, C20 
                 H6 
               
               
                 22 
                 — 
                 119.9, s 
                 — 
                 — 
               
               
                 23 
                 7.55, d (2.6) 
                 153.0, d 
                 C17, C22, C24, C26 
                 H17, H18 
               
               
                 24 
                 8.27, dd (9.8, 2.6) 
                 151.5, d 
                 C23, C26 
                 H18, H25 
               
               
                 25 
                 6.28, d (9.8) 
                 113.7, d 
                 C22, C26 
                 H24 
               
               
                 26 
                 — 
                 164.1, s 
                 — 
                 — 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
         
       
     
       Example 5 
     Bioassays for the Detection of Antitumor Activity 
       [0084]    The aim of this assay is to evaluate the in vitro cytostatic (ability to delay or arrest tumor cell growth) or cytotoxic (ability to kill tumor cells) activity of the samples being tested. 
       Cell Lines 
       [0085]      
         [0000]    
       
         
               
               
               
               
               
             
           
               
                   
               
               
                 Name 
                 N° ATCC 
                 Species 
                 Tissue 
                 Characteristics 
               
               
                   
               
             
             
               
                 A549 
                 CCL-185 
                 human 
                 lung 
                 lung carcinoma (NSCLC) 
               
               
                 HT29 
                 HTB-38 
                 human 
                 colon 
                 colorectal adenocarcinoma 
               
               
                 MDA-MB- 
                 HTB-26 
                 human 
                 breast 
                 breast adenocarcinoma 
               
               
                 231 
               
               
                   
               
             
          
         
       
     
       Evaluation of Cytotoxic Activity Using the SBR Colorimetric Assay 
       [0086]    A colorimetric assay, using sulforhodamine B (SRB) reaction has been adapted to provide a quantitative measurement of cell growth and viability (following the technique described by Skehan et al. J. Natl. Cancer Inst. 1990, 82, 1107-1112). 
         [0087]    This form of assay employs SBS-standard 96-well cell culture microplates (Faircloth et al. Methods in Cell Science, 1988, 11(4), 201-205; Mosmann et al, Journal of Immunological Methods, 1983, 65(1-2), 55-63). All the cell lines used in this study were obtained from the American Type Culture Collection (ATCC) and derive from different types of human cancer. 
         [0088]    Cells were maintained in Dulbecco&#39;s Modified Eagle Medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin and 100 U/mL streptomycin at 37° C., 5% CO 2  and 98% humidity. For the experiments, cells were harvested from subconfluent cultures using trypsinization and resuspended in fresh medium before counting and plating. 
         [0089]    Cells were seeded in 96 well microtiter plates, at 5×10 3  cells per well in aliquots of 150 μL, and allowed to attach to the plate surface for 18 hours (overnight) in drug free medium. After that, one control (untreated) plate of each cell line was fixed (as described below) and used for time zero reference value. Culture plates were then treated with test compounds (50 μL aliquots of 4× stock solutions in complete culture medium plus 4% DMSO) using ten serial dilutions (concentrations ranging from 10 to 0.00262 μg/mL) and triplicate cultures (1% final concentration of DMSO). After 72 hours treatment, the antitumor effect was measured by using the SRB methodology: Briefly, cells were washed twice with PBS, fixed for 15 min in 1% glutaraldehyde solution at room temperature, rinsed twice in PBS, and stained in 0.4% SRB solution for 30 min at room temperature. Cells were then rinsed several times with 1% acetic acid solution and air-dried at room temperature. SRB was then extracted in 10 mM trizma base solution and the absorbance measured in an automated spectrophotometric plate reader at 490 nm. Effects on cell growth and survival were estimated by applying the NCl algorithm (Boyd M R and Paull K D. Drug Dev. Res. 1995, 34, 91-104). 
         [0090]    Using the mean±SD of triplicate cultures, a dose-response curve was automatically generated using nonlinear regression analysis. Three reference parameters were calculated (NCI algorithm) by automatic interpolation: GI 50 =compound concentration that produces 50% cell growth inhibition, as compared to control cultures; TGI=total cell growth inhibition (cytostatic effect), as compared to control cultures, and LC 50 =compound concentration that produces 50% net cell killing (cytotoxic effect). 
         [0091]    Tables 9 and 10 illustrate data on the biological activity of compounds of the present invention. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 Cytotoxicity assay-Activity Data (Molar) 
               
               
                 of Aegomycin A, B, C and D. 
               
             
          
           
               
                   
                 Aegomy- 
                 Aegomy- 
                 Aegomy- 
                 Aegomy- 
               
               
                   
                 cin A 
                 cin B 
                 cin C 
                 cin D 
               
               
                   
                   
               
             
          
           
               
                 MDA-MB- 
                 GI 50   
                 1.91E−8 
                 7.61E−8 
                 6.28E−8 
                 6.48E−8 
               
               
                 231 
                 TGI 
                 3.63E−8 
                 1.67E−7 
                 1.97E−7 
                 9.81E−8 
               
               
                   
                 LC 50   
                 6.11E−8 
                 3.81E−7 
                 6.91E−7 
                 1.73E−7 
               
               
                 HT29 
                 GI 50   
                 1.05E−8 
                 3.04E−8 
                 9.84E−9 
                 3.14E−8 
               
               
                   
                 TGI 
                 3.25E−8 
                 2.28E−7 
                 1.05E−7 
                 1.35E−7 
               
               
                   
                 LC 50   
                 1.15E−7 
                 3.04E−6 
                 &gt;2.09E−5  
                 1.45E−6 
               
               
                 A549 
                 GI 50   
                 3.82E−9 
                 5.90E−9 
                 7.54E−9 
                 8.64E−9 
               
               
                   
                 TGI 
                 4.39E−9 
                 7.61E−9 
                 1.72E−8 
                 1.28E−8 
               
               
                   
                 LC 50   
                 5.35E−9 
                 1.14E−8 
                 4.19E−8 
                 1.83E−8 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 Cytotoxicity assay-Activity Data (Molar) 
               
               
                 of Aegomycin E, F, G and H 
               
             
          
           
               
                   
                 Aegomy- 
                 Aegomy- 
                 Aegomy- 
                 Aegomy- 
               
               
                   
                 cin E 
                 cin F 
                 cin G 
                 cin F 
               
               
                   
                   
               
             
          
           
               
                 MDA-MB- 
                 GI 50   
                 1.97E−7 
                 8.80E−8 
                 1.70E−7 
                 8.11E−8 
               
               
                 231 
                 TGI 
                 3.65E−7 
                 2.71E−7 
                 4.36E−7 
                 2.03E−7 
               
               
                   
                 LC 50   
                 7.50E−7 
                 9.48E−7 
                 1.29E−6 
                 6.08E−7 
               
               
                 HT29 
                 GI 50   
                 1.18E−7 
                 6.55E−8 
                 1.46E−7 
                 3.65E−8 
               
               
                   
                 TGI 
                 4.86E−7 
                 4.06E−7 
                 6.75E−7 
                 2.03E−7 
               
               
                   
                 LC 50   
                 5.27E−6 
                 3.16E−6 
                 3.92E−6 
                 4.86E−6 
               
               
                 A549 
                 GI 50   
                 4.26E−8 
                 2.48E−8 
                 3.92E−8 
                 8.31E−9 
               
               
                   
                 TGI 
                 5.67E−8 
                 3.84E−8 
                 6.32E−8 
                 1.46E−8 
               
               
                   
                 LC 50   
                 7.50E−8 
                 6.10E−8 
                 1.11E−7 
                 2.84E−8