Abstract:
An apparatus and method may be used to separate a biological liquid sample and retrieve a separated liquid portion therefrom. The apparatus may have a tube, an insert, and a positioning mechanism. The tube may have a proximal end, a distal end, and a chamber between the proximal and distal ends for receiving the biological liquid sample. The insert may be positioned in the chamber, and may have a first end, a second end, a well between the first and second ends, and a draw tube in fluid communication with the second end and the well. The positioning mechanism may be connected to the tube and the insert, and may be manually actuable to move the insert between the proximal end and distal end to allow a separated portion of the biological liquid sample to flow proximally from the chamber through the draw tube and collect in the well.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
       [0001]    The present application claims the benefit of U.S. Provisional Application Ser. No. 62/259,413, Attorney&#39;s Docket No. RYO-1 PROV, entitled METHODS AND APPARATUS FOR SEPARATING FLUID COMPONENTS, which was filed on Nov. 24, 2015. The foregoing is incorporated by reference as though set forth herein in its entirety. 
     
    
     TECHNICAL FIELD 
       [0002]    The present invention relates to devices and methods for separating biological fluids into component products. More specifically, the present invention relates to apparatus and methods for concentrating platelet-rich plasma for use in surgical and medical procedures. 
       BACKGROUND 
       [0003]    Platelet-rich blood plasma is required for use in various medical procedures. This blood product is particularly effective due to its growth promoting features, which assist greatly in wound healing and bone regeneration. Blood plasma with a high concentration of platelets can be utilized for dental implants and other periodontal procedures, facial reconstruction, oral or maxillofacial surgery, and chronic wound care, among other uses. In order to obtain a required concentration of platelets, a blood sample is usually centrifuged in order to separate the blood into its component blood products (i.e. plasma, red blood cells and platelets). The platelets, typically in a form of a white “buffy coat,” are then separated from the blood sample and sequestered in concentrated form through aspiration. Conventional aspiration techniques often fail to provide a satisfactory concentration of platelets. 
       SUMMARY OF THE INVENTION 
       [0004]    The various systems and methods of the present invention have been developed in response to the present state of the art, and in particular, in response to the problems and needs in the art that have not yet been fully solved by currently available aspiration systems and methods. The systems and methods of the present invention may provide efficient and effective concentration and retrieval of separated blood products. The devices and methods disclosed herein may be used to separate any suitable fluid; for example blood or bone marrow aspirate (BMA), among others. 
         [0005]    To achieve the foregoing, and in accordance with the invention as embodied and broadly described herein, various systems and methods are disclosed herein, for separating a sample of liquid such as blood contained in a chamber. Such systems and methods may be used to order the phases or fractions of a sample of liquid contained in a chamber by applying a force to the fluid. In particular, an apparatus according to the present disclosure may have a tubular chamber in which a blood sample can be received. Centrifugal force may be applied to the tubular chamber to separate the phases of the blood sample. The one or more separated phases may then be removed from the chamber. 
         [0006]    Specifically, the chamber may be a fluid sample tube having ends separated by a dividing insert. A first end of the tube may receive a sample of fluid. The tube may then be rotated (for example, in a centrifuge) to separate the phases. The dividing insert may be selectively positioned to capture a pre-selected phase of the fluid. The pre-selected phase of the fluid may be received from the first end of the tube. The systems and methods disclosed herein may be used to separate any suitable fluid; for example blood or bone marrow aspirate (BMA), among others. 
         [0007]    In one embodiment, an apparatus for separating a biological liquid sample and retrieve a separated liquid portion therefrom may have a tube, an insert, and a positioning mechanism. The tube may have a proximal end, a distal end, and a chamber between the proximal end and the distal end for receiving the biological liquid sample. The insert may be positioned in the chamber, and may have a first end, a second end, a well between the first end and the second end, and a draw tube in fluid communication with the second end and the well. The positioning mechanism may be connected to the tube and the insert, and may be manually actuable to move the insert between the proximal end and distal end to allow a separated portion of the biological liquid sample to flow proximally from the chamber through the draw tube and collect in the well. 
         [0008]    The positioning mechanism may define a longitudinal axis, and the insert may be translatable relative to the tube and the positioning mechanism along the longitudinal axis. The positioning mechanism may be a lead screw extending from the proximal end into the chamber toward the distal end. The lead screw may pass through the insert. The insert may be movable along a continuum extending from the proximal end toward the distal end, and may be positioned at any selected location along the continuum. The apparatus may further have a lid enclosing the proximal end; the lid may include a first port and a second port displaced from the first port, each port providing selective access into the chamber. The insert may be positioned at the proximal end. The first port may be directly connected to the draw tube to allow the biological liquid sample to be deposited distally through the first port, through the draw tube and into the chamber distal to the insert. When the insert may be distally displaced from the proximal end, the first port may be disconnected from the draw tube. 
         [0009]    The apparatus may further have an O-ring positioned between the insert and the tube to prevent fluid movement between an outer surface of the insert and an inner surface of the chamber. The second end of the insert may be a radially asymmetrical concave distal face. The first end of the insert may be open, and the well may be open toward the proximal end. 
         [0010]    In one embodiment, a method for separating a biological liquid sample and retrieve a separated liquid portion therefrom may include depositing the biological liquid sample into an apparatus including a tube with a proximal end, a distal end, and a chamber between the proximal end and the distal end, and an insert positioned in the chamber, with a first end, a second end, a well between the first end and the second end, and a draw tube in fluid communication with the second end and the well. Depositing the biological liquid sample into the apparatus may include depositing the biological liquid sample into the chamber, distal to the second end. The method may further include centrifuging the tube so that separated liquid collects in the chamber, distal to the second end, actuating a positioning mechanism connected to the tube and the insert, thereby moving the insert distally toward the separated liquid to allow the separated liquid to flow proximally from the chamber through the draw tube and collect in the well, and retrieving the separated liquid from the well. 
         [0011]    The positioning mechanism may define a longitudinal axis. Moving the insert may include translating the insert relative to the tube and the positioning mechanism along the longitudinal axis. Further, the positioning mechanism may include a lead screw extending from the proximal end into the chamber toward the distal end. Actuating the positioning mechanism may include turning the lead screw. Moving the insert distally may include translating the insert from a first position at the proximal end toward the distal end, and stopping the insert at a selected position displaced distally from the proximal end. 
         [0012]    Retrieving the separated liquid may include actuating the positioning mechanism, thereby moving the insert proximally toward the proximal end, and aspirating the separated liquid from the well. A lid may enclose the proximal end. The lid may include a first port and a second port displaced from the first port. Depositing the biological liquid sample into the chamber may include depositing the biological liquid sample through the first port, through the draw tube and into the chamber distal to the insert. Aspirating the separated liquid from the well may include aspirating the separated liquid from the well through the second port. 
         [0013]    The method may further include centrifuging the biological liquid sample a second time. Further, the method may include centrifuging the biological liquid sample a third time, and actuating the positioning mechanism, thereby moving the insert distally toward the separated liquid to allow the separated liquid to flow proximally from the chamber through the draw tube and collect in the well. 
         [0014]    Upon centrifugation of the tube, the biological liquid sample may separate into a first layer, a second layer, and an interface layer between the first and second layers. Retrieving the separated liquid may include retrieving the interface layer. The insert may remain stationary relative to the tube during centrifugation. 
         [0015]    In one embodiment, a method for separating a biological liquid sample and retrieve a separated liquid portion therefrom may include depositing the biological liquid sample into an apparatus including a tube with a proximal end, a distal end, and a chamber between the proximal end and the distal end, and an insert positioned in the chamber, with a first end, a second end, a well between the first end and the second end. The method may further include centrifuging the tube a first time to separate the biological liquid sample into a first layer, a second layer, and an interface layer between the first and second layers, actuating a positioning mechanism to translate the insert toward the interface layer and the distal end, and collecting the interface layer in the well. The method may further include, after actuating the positioning mechanism, centrifuging the tube a second time; after centrifuging the tube the second time, translating the insert toward the proximal end, and after moving the insert toward the proximal end, aspirating the interface layer from the well. 
         [0016]    The method may further include, after centrifuging the tube the second time, actuating the positioning mechanism to move the insert toward the interface layer and the distal end a second time. Further, the method may include, after moving the insert toward the interface layer and the distal end the second time, collecting the interface layer in the well a second time. Yet further, the method may include, after collecting the interface layer in the well the second time, centrifuging the tube a third time. 
         [0017]    These and other features and advantages of the present invention will become more fully apparent from the following description and appended claims, or may be learned by the practice of the invention as set forth hereinafter. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0018]    Exemplary embodiments of the invention will become more fully apparent from the following description and appended claims, taken in conjunction with the accompanying drawings. Understanding that these drawings depict only exemplary embodiments and are, therefore, not to be considered limiting of the invention&#39;s scope, the exemplary embodiments of the invention will be described with additional specificity and detail through use of the accompanying drawings in which: 
           [0019]      FIG. 1A  is an front view of a fluid separation device comprising a tube having proximal and distal ends, a dividing insert, a positioning mechanism, and a lid on the tube proximal end; 
           [0020]      FIG. 1B  is a side transparent view of the device of  FIG. 1A ; 
           [0021]      FIG. 2  is a partially exploded isometric view of the device of  FIG. 1A  from a top down perspective; 
           [0022]      FIG. 3  is a partially exploded isometric view of the device of  FIG. 1A  from a bottom up perspective; 
           [0023]      FIG. 4  is a side cross-sectional view of the device of  FIG. 1A  with the dividing insert in a proximal position wherein a draw tube of the insert is in communication with a first port of the lid; 
           [0024]      FIG. 5  is a front view of the device of  FIG. 1A , with the insert in a proximal position and the tube filled with an unseparated blood sample; 
           [0025]      FIG. 6  is a front view of the device of  FIG. 1A , with the insert in a proximal position and the tube filled with a blood sample which has been centrifuged to separate plasma and red blood cell layers of the sample; 
           [0026]      FIG. 7  is a front view of the device of  FIG. 1A , wherein the insert has been moved toward the distal end of the tube and positioned at an interface between the plasma and red blood cell layers of the blood sample; 
           [0027]      FIG. 8  is a front view of the device of  FIG. 1A , wherein the insert has been moved toward the proximal end of the tube to capture buffy coat and platelet rich plasma in a well of the insert; and 
           [0028]      FIG. 9  is a front view of the device of  FIG. 1A  and a needle, wherein the needle is aspirating the buffy coat and platelet rich plasma from the well of the insert. 
       
    
    
     DETAILED DESCRIPTION 
       [0029]    Exemplary embodiments of the invention will be best understood by reference to the drawings, wherein like parts are designated by like numerals throughout. It will be readily understood that the components of the invention, as generally described and illustrated in the Figures herein, could be arranged and designed in a wide variety of different configurations. Thus, the following more detailed description of the embodiments of the apparatus, system, and method, as represented in  FIGS. 1A through 9 , is not intended to limit the scope of the invention, as claimed, but is merely representative exemplary of exemplary embodiments of the invention. 
         [0030]    The phrases “connected to,” “coupled to” and “in communication with” refer to any form of interaction between two or more entities, including mechanical, electrical, magnetic, electromagnetic, fluid, and thermal interaction. Two components may be functionally coupled to each other even though they are not in direct contact with each other. The term “abutting” refers to items that are in direct physical contact with each other, although the items may not necessarily be attached together. The phrase “fluid communication” refers to two features that are connected such that a fluid within one feature is able to pass into the other feature. 
         [0031]    The word “exemplary” is used herein to mean “serving as an example, instance, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments. While the various aspects of the embodiments are presented in drawings, the drawings are not necessarily drawn to scale unless specifically indicated. 
         [0032]    Referring to  FIGS. 1A, 1B, and 2 , an embodiment of a fluid separation device, or device  100 , is shown. The device  100  includes a sample tube, or tube  10 , a lid  20 , a positioning mechanism  40 , and an insert  50 . The tube  10  may include a proximal or first end  12  which may be open, and a distal or second end  14  which may be blind or closed. A peripheral wall  16  encloses the tube longitudinally. Indicator marks  17  may be present on tube  10 . A tube chamber  18  is bounded by the first end  12 , second end  14 , and peripheral wall  16 . The tube chamber  18  may have a proximal end proximate the first end  12  of the tube  10 , and distal end proximate the second end  14  of the tube  10 . 
         [0033]    The tube  10  and the insert  50  may be formed of any material suitable for maintaining a seal around a fluid. In some examples, the tube  10  and the insert  50  may be formed of a polymer, glass, or other material. Rigid (substantially non-deformable) materials may advantageously be used to help maintain seals and facilitate motion of the insert  50  within the tube  10 . Further, the insert  50  need not be buoyant, as it is not designed to move relative to the tube  10  during the centrifugation process. Rather, the insert  50  may only be designed to move in response to user actuation of the positioning mechanism  40 . 
         [0034]    The exterior of tube  10  may be generally cylindrical. However, as can be seen in  FIG. 2 , the interior of tube  10  may be non-circular in cross section. The interior boundary shape of tube  10  may be complimentarily shaped relative to the exterior of insert  50 . In the embodiment depicted, the interior of tube  10  and exterior of insert  50  are generally oval or D-shaped, and this may prevent rotation of insert  50  within tube  10  during motion of the insert  50  within the tube  10 . 
         [0035]    The lid  20  may include four openings: first port  22  which may be a fill or inlet port, second port  24  which may be an aspiration port, vent  26 , and aperture  28 , which may receive the positioning mechanism  40 . The lid  20  may be removable, or may be permanently joined to the tube  10 . Either or both of the first and second ports  22 ,  24  may be self-sealable or may comprise a plug. In some embodiments, either or both of the first and second ports  22 ,  24  may be sealed through the use of a check valve or other structure permitting fluid to flow in only one direction. Vent  26  may be left open or may be covered and/or plugged. Other embodiments may include more or fewer openings. When properly assembled with tube  10 , first port  22  extend through a boss  30  (shown in  FIG. 3 ) that extends longitudinally into tube  10 . 
         [0036]    In the embodiment depicted, the positioning mechanism  40  comprises a lead screw  42 . When properly assembled with the tube  10 , lead screw  42  extends from the first end  12  through the tube chamber  18  toward the second end  14 . In the embodiment shown, lead screw  42  is solid; i.e., it is not cannulated. Other embodiments of the invention may include a cannulated screw or other positioning mechanism. Lead screw  42  includes a head  44  and threads  46 . A nut  48  may secure lead screw  42  in aperture  28 . Rotation of lead screw  42  about a longitudinal axis  49  in one direction urges insert  50  within tube  10  toward the distal end, i.e., the second end  14 ; rotation of lead screw  42  in the opposite direction urges insert  50  within tube  10  toward the proximal end, i.e., the first end  12 . Thus, the insert  50  may translate relative to the tube  10  and relative to the lead screw, which may remain substantially stationary relative to the tube  10  and the insert  50 , aside from the rotation of the lead screw  42  described above. 
         [0037]    The lead screw  42  may also include indicia, markings, notches and/or other means to provide visual and/or tactile indication of the position of insert  50  within the tube  10 . Additionally or alternatively, the peripheral wall  16  may be translucent so as to enable a user to visualize the position of the insert  50  within the tube chamber  18 . 
         [0038]    Referring to  FIGS. 2-4 , insert  50  includes a first end  52  which may be an open end, a second end  54 , and a peripheral wall  56  extending from the first end  52  to the second end  54 . The insert  50  may also be referred to as a divider, or as a piston. A well  58  occupies the interior of the insert  50 . A passageway  60  passes longitudinally through the insert  50 . In the example shown passageway  60  is threaded to engage with threads  46  on the lead screw  42 . In the example shown, passageway  60  is adjacent to the peripheral wall  56  and isolated from well  58 . A draw tube  62  extends longitudinally along the insert  50 . A first opening  64  of the draw tube  62  may be recessed from the first end  52  of the insert  50 . A second opening  66  of the draw tube  62  opens out on the second end  54  of the insert  50 . The second end  54  of the insert  50  may comprise a distal face  68 , which is concave and may urge fluid flow toward the second opening  66  of the draw tube  62 . In the example shown, the distal face  68  is radially asymmetrical and non-conical. The insert  50  may further include a groove  70 , which can house an O-ring  72  (shown in  FIG. 5 ). Insert  50  may also be translucent so that the levels of fluids in the draw tube and the well  58  can be observed. 
         [0039]    In one method of use, the device  100  is used with a centrifuge to separate and isolate a phase of a liquid, such as blood. Any of a wide variety of centrifuge types may be used; according to an example, the device  100  may be used with the model  5207  centrifuge available from Eppendorf Belgium N.V.-S.A. The method may be used to separate, concentrate and collect the buffy coat layer which forms between red blood cells (RBC) and plasma layers during centrifugation. In additional, platelet rich plasma (PRP) may also be collected. The device  100  may be provided in a sterile package with the insert  50  pre-positioned at the top, i.e., proximate the first end  12  of the device so that it is ready to fill. 
         [0040]    Referring to  FIGS. 5-9 , the device  100  may be filled with 30-60 cc of whole blood  90  (anticoagulated) using a syringe connected to the first port  22  at the first end  12  of the device  100 . The first port  22  may only be in fluid communication with the draw tube  62  for filling when the insert  50  is in an upward or proximal position within the tube  10 , as shown in  FIG. 5 . In this position, the first port  22  may be in direct fluid communication with the draw tube  62 , for example, via engagement of the boss  30  with the first opening  64  of the draw tube  62 . Further, the first end  52  of the insert  50  may be in direct contact with the first end  12  of the tube, and/or in direct contact with the lid  20 . 
         [0041]    The device  100  is weighed and placed in the centrifuge, which may be any of a wide variety of commercially available centrifuges. A counterbalancing weight in the centrifuge may be added and/or adjusted to match the device weight. The device  100  is spun a first time, which may be for a duration of four minutes at 3,500 RPM, and removed from the centrifuge. The four-minute duration is merely exemplary; in other embodiments, a different duration may be used, and may range from 30 seconds to ten minutes. More specifically, the duration may range from one minute to eight minutes. Yet more specifically, the duration may range from two minutes to six minutes. Similarly, the 3,500 RPM speed is merely exemplary; in other embodiments, a different centrifugation speed may be used, and may range from 2,000 RPM to 5,000 RPM. More specifically, the centrifugation speed may range from 2,500 RPM to 4,500 RPM. Yet more specifically, the centrifugation speed may range from 3,000 RPM to 4,000 RPM. 
         [0042]    As a result of the centrifugation, the blood may be separated with erythrocytes, which are red blood cells  92  (RBC) at the bottom and plasma  94  at the top. As shown in  FIG. 6 , an interface layer, or a buffy coat layer  96 , is located at the interface between the red blood cells  92  and plasma  94  and contains a large portion of the platelets and white cells. The buffy coat layer  96  may contain white blood cells (leucocytes) and platelets. The insert  50  may not move relative to the tube  10  during centrifugation; rather, it may remain fixed in position relative to the tube  10 . 
         [0043]    Referring to  FIG. 7 , following centrifugation, the lead screw  42  may be turned counter-clockwise to urge the insert  50  toward the second end  14  of the tube  10 . A hand knob  80  may be provided for ease of turning the lead screw  42 . The hand knob  80  may optionally be a separate piece from the device  100 , and may have an interface (not shown) that enables the hand knob to be coupled to the head  44  of the lead screw  42 . 
         [0044]    As the insert  50  is lowered into the fluid (for example, into the plasma  94 ), a portion of the plasma  94  flows up through the draw tube  62 . A user may be able to observe the plasma  94  as it flows upward though the draw tube  62 , exiting opening  64  into the well  58  of the piston. When the buffy coat layer  96  has exited the top of the draw tube  62  into the well, a predetermined amount of red blood cells  92  can be allowed to exit the draw tube  62  as well, depending on the desired amount of red blood cells  92  in the final product. When the desired amount of the buffy coat layer  96  and any additional red blood cells  92  have exited through the draw tube  62 , the user may cease turning the lead screw  42  to stop movement of the insert  50  relative to the tube  10 . 
         [0045]    The device  100  may then be placed back into the centrifuge. The centrifuge may then be activated to spin the device  100  for a second time. The counterbalancing weight may not need adjustment, since the total amount of fluid in the device  100  has not changed; i.e., fluid has not been added or removed. In one embodiment of the method in which a second spin is used, the second spin may be for five minutes at 3,800 RPM. As with the first spin, this duration and centrifugation speed are merely exemplary; the range of values for duration and centrifugation speed provided for the first spin is also applicable to the second spin. 
         [0046]    If a two-spin protocol is followed, following the second spin, the device  100  may be removed from the centrifuge. Referring to  FIGS. 8 and 9 , insert  50  may be raised toward the first end  12  by turning the lead screw  42  clockwise, for example, via the hand knob  80 . This may allow the top portion of the plasma  94 , which may be Platelet Poor Plasma (PPP), to drain back under the insert  50 , leaving some of the buffy coat layer  96  and the remainder of the plasma  94  in the well  58  of the insert  50 . In some embodiments, 7 cc of fluid may remain in the well  58 , which may include some or all of the buffy coat layer  96  along with the remainder of the plasma  94 , which may generally be Platelet Rich Plasma (PRP). The insert  50  may be raised until the level of PRP in the well  58  is below the first opening  64  of the draw tube  62 ; additionally or alternatively, the insert  50  may be raised until an air gap can be seen under the insert  50 , between the insert  50  and the column of fluid. 
         [0047]    A spinal needle  98  or other aspiration device may then be inserted through the second port  24  on the lid  20  of the device  100 , and the 7 cc of fluid in the well  58  may be aspirated into a syringe  102  or other container. The fluid may be forced through the syringe  102  and back into the well  58  of the insert  50  to enhance mixing; this step may be repeated several times. In one embodiment, this step is repeated three times. In other embodiments, it may be repeated more or fewer than three times. After the final aspiration, the product in the syringe  102  may be well-mixed and ready for clinical application. 
         [0048]    If a three-spin protocol is followed, after the second centrifuge spin, the device  100  may be removed from the centrifuge, and the lead screw  42  may again be turned counter-clockwise to urge the insert  50  to move further toward the second end  14  of the tube  10 . The user may be able to observe the fluid as it flows upward though the draw tube  62  into the well  58  of the insert  50 . When the buffy coat layer  96  has exited the top of the draw tube  62 , a predetermined amount of the red blood cells  92  can be allowed to exit the draw tube  62  as well, depending on the desired amount of red blood cells  92  in the final product. When the desired amount of the buffy coat layer  96  and any additional red blood cells  92  have exited through the draw tube  62 , the user may cease turning the lead screw  42  to stop movement of the insert  50  relative to the tube  10 . 
         [0049]    The device  100  may be placed back into the centrifuge and spun for a third time. Again, the counterbalancing weight may not need adjustment, since no fluid has been removed from the device  100 . The third spin may be for 1.5 minutes at 3800 rpm. As with the first and second spins, this duration and centrifugation speed are merely exemplary; the range of values for centrifugation speed provided for the first spin is also applicable to the second spin. Different durations may be used, and may range from 30 seconds to five minutes. More specifically, the duration may range from one minute to three minutes. Yet more specifically, the duration may range from one-and-a-quarter minutes to two minutes. 
         [0050]    In some embodiments, the durations and/or centrifugation speeds of the first spin and/or the second spin may be modified for the three-spin protocol. For example, the first spin and the second spin may each be carried out at 3500 RPM for two minutes. In such a case, the third spin may be performed at 3800 RPM for five minutes, like the second spin of the two-spin protocol. These durations and centrifugation speeds are merely exemplary; alternative durations and/or centrifugation speeds may be used, as set forth in the preceding descriptions of the first, second, and third spins. 
         [0051]    Following the third spin, the device  100  may be removed from the centrifuge. The insert  50  may be raised toward the first end  12  by turning the lead screw  42  clockwise. The insert  50  may be raised until the level of PRP in the well  58  of the insert  50  is below the first opening  64  of the draw tube  62 ; additionally or alternatively, the insert  50  may be raised until an air gap can be seen under the insert  50 , between the insert  50  and the column of fluid. This may allow the top portion of the plasma  94 , which may be Platelet Poor Plasma (PPP), to drain back under the insert  50 , leaving about 7 cc of the buffy coat layer  96  and PRP in the well  58  of the insert  50 . The spinal needle  98  or other aspiration device may then be inserted through the second port  24  on the lid  20  of the device  100 , and the 7 cc of fluid may be aspirated into the syringe  102 . The fluid may be forced through the syringe  102  and back into the well  58  of the insert  50  to enhance mixing; this step may be repeated several times. In one embodiment, this step is repeated three times; in other embodiments, it may be repeated more or fewer than three times. On the final aspiration, the product in the syringe  102  may be well-mixed and ready for clinical application. 
         [0052]    In other embodiments of the method, more or less than 7 cc of fluid may be aspirated. Further, in other embodiments of the method, more than three centrifuge spins may be included to further concentrate the platelet rich plasma. The spin times and RPMs may vary, as described previously. 
         [0053]    The two-spin protocol and the three-spin protocol may both be carried out through use of the device  100 . The three-spin protocol may have the potential to recover more platelets, thus providing a higher platelet concentration in the final product. The two-spin protocol may be faster while still providing adequate platelet concentration for many applications. 
         [0054]    The devices and methods described herein may be used with other fluids, for example bone marrow aspirate, cell suspensions, and/or any other fluid that is separable into fractions. Although the method refers to a blood separation, it will be understood that the present invention may be used to separate and concentrate any appropriate fluid. 
         [0055]    Any methods disclosed herein comprise one or more steps or actions for performing the described method. The method steps and/or actions may be interchanged with one another. In other words, unless a specific order of steps or actions is required for proper operation of the embodiment, the order and/or use of specific steps and/or actions may be modified. 
         [0056]    Reference throughout this specification to “an embodiment” or “the embodiment” means that a particular feature, structure or characteristic described in connection with that embodiment is included in at least one embodiment. Thus, the quoted phrases, or variations thereof, as recited throughout this specification are not necessarily all referring to the same embodiment. 
         [0057]    Similarly, it should be appreciated that in the above description of embodiments, various features are sometimes grouped together in a single embodiment, Figure, or description thereof for the purpose of streamlining the disclosure. This method of disclosure, however, is not to be interpreted as reflecting an intention that any claim require more features than those expressly recited in that claim. Rather, as the following claims reflect, inventive aspects lie in a combination of fewer than all features of any single foregoing disclosed embodiment. Thus, the claims following this Detailed Description are hereby expressly incorporated into this Detailed Description, with each claim standing on its own as a separate embodiment. This disclosure includes all permutations of the independent claims with their dependent claims. 
         [0058]    Recitation in the claims of the term “first” with respect to a feature or element does not necessarily imply the existence of a second or additional such feature or element. Elements recited in means-plus-function format are intended to be construed in accordance with 35 U.S.C. §112 Para. 6. It will be apparent to those having skill in the art that changes may be made to the details of the above-described embodiments without departing from the underlying principles of the invention. 
         [0059]    While specific embodiments and applications of the present invention have been illustrated and described, it is to be understood that the invention is not limited to the precise configuration and components disclosed herein. Various modifications, changes, and variations which will be apparent to those skilled in the art may be made in the arrangement, operation, and details of the methods and systems of the present invention disclosed herein without departing from the spirit and scope of the invention.