Abstract:
Screening of crude extracts of the bark of Annona bullata Rich. (Annonaceae) showed cytotoxic and pesticidal activities. By monitoring with brine shrimp lethality, two novel extremely potent acetogenins, bullatacin (1) and bullatacinone (2), were isolated. Spectral and chemical methods identified bullatacin as a diastereomer of asimicin. Bullatacinone represents bullatacin with the lactone cleaved and reformed at the 4-OH. Unlike asimicin, which is more generally cytotoxic, 1 and 2 show some selective cytotoxicities in human tumor cell lines, and certain susceptible cells give ED 50  values as low as 10 -12  -10 -15  mcg/ml. Bullatacin was pesticidal at concentration as low as 1 ppm, while bullatacinone lacked pesticidal activities.

Description:
This is a continuation of Ser. No. 07/336,233 filed on Apr. 11, 1989, now abandoned. 
    
    
     BACKGROUND OF INVENTION 
     This invention relates to two acetogenin compounds isolated in substantially pure form from Annona bullata Rich. (Annonaceae). The new compounds, designated herein as bullatacin and bullatacinone, have been found to exhibit antitumor and pesticidal activity. 
     DESCRIPTION OF PRIOR ART 
     Bis(tetrahydrofuranoid) fatty acid lactones, represented by the structural formula, ##STR1## have been reported in the literature as having cytotoxic and antitumor activity. The first such compound reported [Jolad, et al., J. Org. Chem, 47: 3151-3153 (1982)] was called uvaricin and was characterized by an --OH group in the R 2  position and an acetoxy group in the R 3  position, with R 1  and R 4  being hydrogen. Uvaricin was isolated from the roots of Uvaria accuminata of the family Annonaceae and demonstrated activity in vivo against P-388 (PS) lymphocytic leukemia in mice. Subsequently, Jolad et al. [J. Nat. Prod., 48: 644-645 (1985)] disclosed the compound desacetyluvaricin which differs from uvaricin in having an hydroxyl in the R 3  position. Dabrah and Sneden [Phytochemistry 23: 2013-2016 (1984)] showed the isolation of the undefined stereoisomers, rollinicin, and isorollinicin from the roots of Rollinia papilionella (Annonaceae). These compounds were reported as having --OH in R 2 , R 3  and R 4  positions with R 1  being hydrogen. Both of these compounds exhibited in vitro cytotoxicity against the P-388 lymphocytic leukemia. Etse and Waterman J. Nat. Proc., 684-686 (1984)] reported the isolation and structure of 14-hydroxy 25-desoxyrollinicin which differs from rollinicin in having an hydroxyl in R 1  and a hydrogen in R 4 . There is no report for the bioactivity of this compound. Dabrah and Sneden [J. Nat. Prod., 47: 652-657 (1984)] disclosed another member of the series referred to as rollinone. Rollinone is characterized by a keto group at the R 1  carbon, hydroxyls at R 2  and R 3 , and hydrogen for R 4  with a saturated lactone ring as illustrated in structure IA: ##STR2## Rollinone demonstrated both cytotoxicity against P-388 in vitro and also activity in vivo against the same system in mice. The compounds having the general structure designed by Formula I, above, have acquired the name &#34;linear acetogenins&#34;. 
     Cortes et al. [Tetrahedron Lett. 25: 3199-3202 (1984)] described two additional linear acetogenins from Annona cherimolia (Annonaceae), wherein the structures are reported as follows: ##STR3## These compounds have antimicrobial activity as demonstrated against Gram negative bacteria and Candida. 
     Rupprecht et al. [Heterocycles 24: 1197-1201 (1986)] and Mikolajczak et al., U.S. Pat. No. 4,721,727, disclosed a new member of this series referred to as asimicin. Asimicin was isolated from Asimina triloba Dunal. (Annonaceae) and is characterized by two hydroxyl groups in the R 2  and R 3  positions, two hydrogens in the R 1  and R 4  positions and a hydroxyl group at carbon 4, found for the first time for this type of compound, as illustrated in partial structure (IB). ##STR4## Asimicin was toxic to mice at 6.25 mg/kg and active (124% T/C at 0.0125 mg/kg) in the 3PS lymphocytic leukemia system and possesses cytotoxicities in the 9KB (human nasopharyngeal carcinoma, ED 50  &lt;10 -5  μg/ml) and the 9PS (murine lymphocytic leukemia, ED 50  &lt;10 -7  μg/ml systems. Promising pesticidal activity against the stripped cucumber beetle, Mexican bean beetle, mosquito larvae, blow fly larvae, melon aphid, two spotted spider mites, and the free-living nematode, Caenorabditis elegans, was demonstrated and this pesticidal use of the acetogenins was patented on Jan. 26, 1988 [Mikolajczak et al., U.S. Pat. No. 4,721,727]. An uncharacterized pesticidal substance called annonin and a process for its isolation from the seeds of Annona souamosa (Annonaceae) has been patented by Moeschler et al. [U.S. Pat. No. 4,689,232]. 
     Pettit et al. (Can. J. Chem., 65: 1433-1435 (1987)] isolated a diastereomer of asimicin from Rollinia mucosa (Annonaceae). This diastereomer is called rolliniastatin. Its stereochemistry, which was revealed by the first X-ray crystallographic analysis of this type of compound, is threo, cis, threo, cis, erythro, 4S and 36S and differed from asimicin which is threo, trans, threo, trans, and threo as analyzed by the  1  H nmr analysis method developed by Hoye et al. [J. Am. Chem. Soc., 109: 4402-4403(1987)]. Rollinia mucosa has been known in primitive medical practices of Indonesia and the West Indies as a treatment for tumors. Biological evaluation of rolliniastatin showed PS activities: 28% life extension and ED 50  4.5×10 -5  μg/m in cell culture. 
     SUMMARY OF THE INVENTION 
     During the screening of plants in our laboratory, we have unexpectedly discovered that Annona bullata Rich. in the Annonaceae family has noteworthy activities in the BST (brine shrimp lethality test), PD (crown gall antitumor activity on potato discs), 9PS (murine leukemia cytotoxicity), 9KB (human nasopharyngeal carcinoma cytotoxicity), and 9ASK (astrocytoma reversal) bioassays. In addition, activities in several pesticidal tests at Eli Lilly showed promise. The compounds found to correspond to these activities as well as selective antitumor activities were the class of natural bistetrahydrofuranoid fatty acid lactones called acetogenins. The substantially pure compounds in accordance with this invention, bullatacin and bullatacinone, have been characterized to be two new members of this unusual class of compounds. Both of these compounds have the same stereochemistry at their corresponding tetrahydrofuran rings and the two adjacent hydroxyl groups. Bullatacin is characterized by a hydroxyl group on carbon 4 and a terminal α, β-unsaturated lactone ring. Bullatacinone is characterized by a terminal methyl ketone and a lactone ring formed with a carbon 4 hydroxyl group. The structural formulas of the present compounds are illustrated below as (1) and (2). ##STR5## 
     It is an object of this invention to provide the two Annonaceous acetogenin compounds designated as bullatacin and bullatacinone in substantially pure form. 
     It is also an object of this invention to provide two of the most active acetogenins known today in in vitro human tumor cell lines. 
     It is still a further object of this invention to provide a method for converting bullatacin to bullatacinone, bullatacinone exhibiting a more selective antitumor activity. 
     It is a further object of the invention to provide bullatacin as a new and potent pesticide in the acetogenin group. 
     Other objects and advantages of the invention will become readily apparent from the ensuing description. 
     DETAILED DESCRIPTION OF THE INVENTION 
     The starting material for use in the invention is the bark of Annona bullata Rich. (Annonaceae), and it is considered likely, by the screening of other parts of the plant, that other tissues such as twigs, wood, roots, seeds and leaves would also contain extractable quantities of the subject compounds. 
     The bark material is prepared for extraction by grinding in a conventional mill to a suitable particle size, usually in the range of about 0.001-3 mm. in diameter, and more preferably in the range of 0.1-2 mm. The ground material is extracted by percolating with 95% EtOH. The ethanol solubles are concentrated to remove the bulk of the solvent, at least to the point of reducing the extract to a thick syrup. The resultant concentrate is partitioned between water and a water-immiscible solvent, such as chloroform, in order to remove the water solubles which are freeze dried and labelled F002. The chloroform solubles are recovered as a syrup residue using a solvent evaporator and labelled as F003. The insoluble interface was dried at ambient temperature and labelled F004. F003 then is partitioned between hexane and 90% aqueous MeOH in order to remove hexane solubles which are vacuum dried and labelled as F006. The 90% aqueous MeOH solubles are recovered by vacuum evaporation to a thick syrup as a crude acetogenin-containing extract F005. 
     Separation and purification of pure acetogenins from the crude extract (F005) can be affected by the use of the proper combination of conventional techniques including, for example, column chromatography (CC), thin-layer chromatography (TLC), medium-pressure chromatography (MPC) and chromatotrons. While not desiring to be limited thereto, the details of the separation procedure are illustrated by the following examples. Fractionation of the ethanolic extract was guided by assay with the brine shrimp lethality test (BST) and confirmed by assays on tumor cell cultures. Pesticidal tests were conducted at Eli Lilly Laboratories (Greenfield) in indicator organisms following standard procedures. 
    
    
     EXAMPLES 
     Bioassay Procedure 
     The following bioassays were used to guide the phytochemical fractionation described below. 
     The extracts, fractions, and isolated compounds were routinely evaluated for lethality to brine shrimp larvae (BST). The LC 50  of brine shrimp (Artemia salina Leach) was determined by substantially the same method described by Meyer et al. [Planta Med., 45: 31-34 (1982)]. The test sample was dissolved in solvent and added to 2-dram vials in an amount to provide 1000, 100, 10, 1, etc. p.p.m. of material in the final brine preparation, assuming complete miscibility in the brine. The vials were dried in vacuo, and artificial sea water, prepared from a commercial salt mixture, was added. Ten brine shrimp larva (nauplii), 48-72 hrs. old, were introduced to the vial, and the volume was made up to 5 ml. with the sea water. After 24 hrs., the percent mortality was computed. Subsequently, LC 50  values and 95% confidence limits were calculated by a Finney probit analysis to permit comparison of potencies of extracts and fractions. 
     Occasional checks in the potato disc (PD) assay assured us that antitumor activity was present [Ferrigni et al., J. Nat. Prod., 45; 679-686 (1982)]. Cytotoxicity tests were performed at the Purdue Cell Culture Laboratory, Purdue Cancer Center, using standard protocols for 9KB (human nasopharyngeal carcinoma) and 9PS (a chemically-induced murine lymphocytic leukemia) [Suffness et al., Methods In Cancer Research. Vol. 16: 73 (1979), V. T. DeVita, Jr. and H. Busch eds., Academic Press, New York], 9ASK (astrocytoma reversal) [Lgarashi et al., Cell Struct. and Funct., 3: 107 (1978)], A-549 (human lung carcinoma) [Giard et al., J. Nat. Cancer Inst., 51: 1417-1423 (1973)], and HT-29 (human colon adenocarcinoma) [Fogh J. (ed.), Human Tumor Cells, In Vitro, Plenum Press, New York: 115-159 (1975)]. 
     Isolated pure compounds were sent to the National Institute of Health, National Cancer Institute, Bethesda, Md., for testing in human cancer cell line panels including leukemia, non-small cell lung cancer, small cell lung cancer, CNS cancer, melanoma, ovarian cancer and renal cancer. 
     Pesticidal bioassays were conducted at Eli Lilly Laboratories (Greenfield, Ind.) following standard procedures with eight indicator organisms: mosquito larvae (ML) Aedes aegypti (in the media), blowfly larvae (BFL) (1% in the diet), corn root worm (CRW) Diabrotics undecimcuntata howardii (in soil), two-spotted spider mite (2SSM) Tetranychus urticae (on foliage), southern army worm (SAW) Spodoptera eridania (on foliage), melon aphid (MA) (5000 ppm on foliage), cotton aphid (CA) Aphis gossypii, (on foliage) and Haemonchus contortus (HC) (a nematode, 0.1% in the media). 
     Isolation of the Compounds 
     A. Extraction Procedures 
     Approximately 3.9 kg of Annona bullata Rich. (Annonaceae) bark (M-06983, PL-103519) was collected by Edward Garvey at the USDA Subtropical Horticulture Research Station, ARS, 13601 Old Culture Rd., Miami, Fla. 33158. The tree originated from seeds collected in Cuba in 1933 by Robert M. Grey of Harvard University. Air-dried bark was pulverized through a 2 mm screen in a Wiley mill. The pulverized bark was extracted by exhaustive percolation with 777 liters of 95% EtOH. Vacuum evaporation left 380 g of syrupy residue (F001). F001 was partitioned between CHCl 3  H 2  O (1:1), and the water solubles were freeze dried and labelled F002 (11 g). The chloroform solubles were vacuum evaporated to form F003 (181 g). The insoluble interface was air dried and labelled F004 (188 g). Then F003 was partitioned between hexane/90% aqueous MeOH (1:1). The 90% MeOH fraction was vacuum evaporated to a thick syrup and labelled F005 156 g.) The hexane residue (25 g) was labelled F006. The bioassay data (Table 1) clearly showed that the most activity was concentrated in the 90% MeOH fraction (F005). 
     
                                           TABLE 1__________________________________________________________________________Bioactivities of Initial Fractions from Annona bullata Rich.BSTLC.sub.50 mcg/ml95%                                 Protein kinase CConfidence     PD     9KB    9PS    9ASK % DisplacementInterval  % Inhibition            ED.sub.50  mcg/ml                   ED.sub.50  mcg/ml                          reversal                               100 mcg/ml__________________________________________________________________________F001   0.0062 --.sup.a            20     &lt;10.sup.-2                          not  11%   0.0120                      active   ↓   0.0009F002   &gt;100   --     &gt;10    &gt;10    --   --F003   0.0030   0.0036 --     10.sup.-1 -10.sup.-2                   &lt;10.sup.-2                          not  67%   ↓                    active   0.0010F004   5.4000 --     &gt;10    2.11   --   --   838→1.53F005   0.0025   0.0050 78%    &lt;10.sup.-5                   &lt;10.sup.-2                          slight                               68%   ↓                    active   0.0001F006   &gt;100   --     &gt;10    4.10   --   --__________________________________________________________________________ .sup.a A dash () indicates that tests were not conducted. 
    
     B. Chromatographic Separations 
     F005 (80 g) was absorbed onto 100 g of Celite and applied to a column of Si gel (3 kg) packed in a slurry of hexane. A gradient of hexane--CHCl 3  --MeOH was used to elute the column, collecting 82 fractions of 100-200 ml each. Fractions were combined into pools according to their similar TLC patterns [CHCl 3  --MeOH (9:1) on Si gel, phosphomolybdic acid spray], weighed, and bioassayed by the BST. 
     The largest and the most toxic pool (P40-51, 25 g) was absorbed onto 100 g of Celite and chromatographed over a column of 4.4 kg of Si gel packed as a slurry in CHCl 3 . A gradient of CHCl 3  --EtOAc--MeOH was used to elute the column, collecting fractions of 100-200 ml. Pools were made after TLC and bioassayed in the BST. From the most toxic pool (P106-111), which had been eluted with CHCl 3  --EtOAc (1:1), a white precipitate was obtained. Recrystallization from EtOAc gave fine white needles which were labelled as bullatacin (1) (100 mg); this material was homogeneous in several TLC systems. From P17-40, which had been eluted with CHCl 3 , another white precipitate appeared which was recrystallized from EtOAc to a white homogeneous solid (5 mg), later designated bullatacinone (2). 
     Pool 31-39, from the first column, stood for some time. Orange crystals formed and were recrystallized from MeOH to yield yellow needles (150 mg); this material was identified (mp, ir, ci and eims) as liriodenine [Lopez et al. Rev. Cubana Farm., 20: 83-86 (1986) (Span.)]. The residue (5.7 g) of the mother liquor was chromatographed over Si gel at medium pressure in a Michel-Miller column, eluted with a gradient of CHCl 3  --MeOH. A white solid formed in pool 104-113; this was recrystallized from EtOAc to yield fine white needles (8 mg) of additional bullatacinone (2). 
     Upon standing, pool 8-17 from the first chromatography column gave large colorless crystals. After recrystallization from MeOH, these were identified (uv, ei and cims, hrms,  1  H and  13  C nmr) as (-)-kaur-16-en-19-oic acid [Leboeuf et al., Phytochemistry, 21: 2783-2813 (1982)]. 
     Characterization of Compound Structures 
     A. Structural Characterization of Bullatacin (1) 
     Mp 69-70°; [α]23°/589=+13.00, [α]23°/578=+14.70, [α]23°/546=+19.04, [α]23°/436=+36.63, [α]23°/365=+66.99 (c, 0.004, CHCl 3 ); cims (isobutane) m/z 623 (MH + ), cims (ammonia) m/z 622 (M+NH +   4  --H 2  O), 640 (M+NH +   4 ), eims m/z 622 (M + ); hr cims 623.4847 (calc. 623.4889) for C 37  H 66  O 7  ; uv (EtOH) λ max 215.5 nm (ε=7974); ir (KBr) cm -1  3430 (hydroxyl), 1750 (carbonyl). 
     These spectral characteristics indicated that 1 belongs to the familiar class of bioactive bistetrahydrofuran acetogenins which contain 37 carbons and two long hydrocarbon chains, one of which terminates with a γ-lactone. 
     The structure of fragment A, which contains the lactone ring and one of the three hydroxyl groups, was elucidated by high field  1  H nmr (Table 2),  13  C nmr (Table 3), and ms spectral analyses. 
     
                                           TABLE 2__________________________________________________________________________.sup.1 H NMR Assignments and Comparisons of Compounds.*__________________________________________________________________________Bullaticin (1)  Bullatacin (1)                       Bullatacinone (2)470 MHz, CDCl.sub.3           470 MHz, C.sub.6 D.sub.6                       470 MHz, C.sub.6 D.sub.6__________________________________________________________________________2     --          --        2.71dddd2, 3a(9.34)                       2, 3b(9.34)                       2, 35b(3.42)                       2, 35a(9.34)3a  2.50 dddd 3a, 3b(15.0)           2.30 dddd 3a, 3b(15.0)                       1.70ddd 3a, 3b(12.82)    3a, 4 (4.0) 3a, 4 (4.0) 3a, 4 (3.38)    3a, 35(1.5) 3a, 35(1.5) 3a, 2 (9.34)    3a, 36(1.1) 3a, 36(1.1)3b  2.36 ddt 3b, 3a(15.0)           2.20 ddt 3b, 3a(15.0)                       1.40ddd    3b, 4 (8.0) 3b, 4(8.0)    3b, 35(1.4) 3b, 35(1.4)4   3.80m       3.71br tt   4.05m5   1.3-2.0     1.3-2.1     2.3-2.06-13    1.25br s    1.25br s    1.25br s14  1.35m       1.3-2.1     1.40, 1.5515  3.38tt 15, 16 (8.0)           3.45ttt 15, 16 (8.0)                       3.49tt 15, 16 (8.06)    15, 14 (2.0)           15, 14 (2.0)                       15, 14 (2.38)           15, 17 (1.0)16  3.83m       3.85ddd 16, 17a(8.0)                       3.85ddd 16, 17a(8.06)           16, 17b(6.9)                       16, 17b(6.96)           15, 17 (1.0)17, 18    1.3-2.0     1.3-2.1     1.65, 1.3519x 3.92m       3.89m       3.89m20x 3.83m       3.67ddd 20, 21a(6.0)                       3.66ddd 20, 21a(5.96)           20, 21b(1.0)                       20, 21b(1.00)           20, 19(15.0)                       20, 19(14.93)21, 22    1.3-2.0     1.3-2.1     1.52, 1.4223y 3.92m       3.99dt 23, 24(8.5)                       3.95dd 23, 24(8.4)           23, 22(2.5) 23, 22(2.5)24y 3.83m       3.89m       3.89m25  1.3-2.0     1.3-2.1     2.10, 1.6026-33    1.25br s    1.25br s    1.25br s34  0.85t 34, 33(6.81)           0.9t 34, 33(6.81)                       0.90t 34, 3 (7.05)35a                         1.93dd 35a, 35b(18.31)    7.17d 35, 36(1.70)           6.25d 35, 36(1.70)                       35a, 2 (9.34)35b                         2.53dd 35b, 2 (3.42)36  5.05ddq 36, 37(6.83)           4.12ddq 36, 37(6.83)                         --37  1.41d       0.80d       1.55s__________________________________________________________________________    Rolliastatin           Asimicin    Asimicin    300 MHz, CDCl.sub.3           470 MHz, CDl.sub.3                       470 MHz, C.sub.6 D.sub.6__________________________________________________________________________2     --          --          --3a  2.50 dddd 3a, 3b(15.1)           2.51 dddd 3a, 3b(15)                       2.35    3a, 4 (3.5) 3a, 4 (3.5)    3a, 35(1.5) 3a, 35(1.5)    3a, 36(1.6) 3a, 36(1.7)3b  2.36 dddd   2.38ddt 3b, 4 (8.0)                       2.27    3b, 4 (8.1) 3b, 35(1.5)    3b, 35(1.2) 3b, 36(1.5)    3b, 36(1.54   3.85m       3.86m       3.775   1.45        1.55m       1.4-1.56-13    1.25        1.25m       1.3514  1.50m       1.55m       1.5515  3.38m       3.37br, q   3.4516  3.85m       3.79-3.85m  3.8617, 18    1.7-1.9m    1.6-2.0m    1.4-1.819, 20    3.85m       3.79-3.89m  3.7321, 22    1.7-1.9m    1.6-2.0m    1.4-1.823, 24    3.85m       3.79-3.89m(23)                       3.86(23)           3.37br, q (24)                       3.45(24)25  1.45m       1.55m       1.5526-33    1.25br, s   1.25m       1.3534  0.85t       0.86t       0.9035  7.16ddd     7.17q       6.3536  5.02dddq    5.06qq      4.337  1.40d       1.41d       0.86__________________________________________________________________________ *Some coupling constants were obtained by decoupling experiments x, y Indicate that assignments may be interchangable 
    
     
                       TABLE 3______________________________________.sup.13 C NMR (CDCl.sub.3) Assignments and Comparisons..sup.aCarbon Bullatacin BullatacinoneNo.   (50 MHz)(1)            (50 MNz)(2) Rolliniastatin                                 Asimicin______________________________________1     174.51s    178.73s     174.5s   174.6s2     131.11s    44.18d      131.1s   131.1s3.sup.a 33.23t     34.41t      33.2t    33.4t4     69.91d     78.86d      69.9d    69.9d5.sup.a 37.34t     36.67t      37.4t    37.5t6.sup.a 25.99t     25.99t      26.0t    25.6t7-12.sup.a 29.92t     29.56t      29.5t    29.6t 29.28t     29.37t      29.3t    29.3t13.sup.a 25.54t     25.22t      25.7t    25.6t14.sup.a 33.23t     33.19t      34.1t    33.6t15    74.08d     74.10d      74.0d    74.1d16    83.20d     83.26d      83.0d    83.1d17.sup.a 28.91t     28.92t      28.7t    28.4t18.sup.a 28.37t     28.37t      27.8t    28.4t19    82.44d     82.44d      81.1d    81.7d20    82.17d     82.19d      81.0d    81.7d21.sup.a 28.91t     28.92t      27.8t    28.4t22.sup.a 28.37t     28.37t      28.4t    28.4t23    82.75d     82.77d      83.0d    82.8d24    72.34d     71.26d      71.8d    74.1d25.sup.a 32.38t     32.37t      32.8t    33.4t26.sup.a 25.54t     25.59t      25.5t    25.6t27-31.sup.a 29.49t     29.56t      29.6t    29.6t 29.28t     29.25t      31.9t    29.3t32.sup.a 31.83t     31.85t      31.9t    31.9t33    22.62t     22.63t      22.6t    22.7t34    14.10q     14.05q      14.1q    14.1q35    151.70d    35.42t      151.7d   151.6d36    77.88d     205.44s     77.9d    77.9d37    19.06q     24.46q      19.1q    19.1q______________________________________ .sup.a Assignments of similar signals, as indicated, may be interchanged. 
    
     A positive response to Kedde&#39;s reagent, the strong ir absorption at 1750 cm -1  and the uv absorption maximum at 215.5 nm (ε=7974) in EtOH suggested the presence of an α,β-unsaturated γ-lactone.  1  H-- 1  H decoupling experiments between protons 3 and proton 4 in the 470 MHz ) (C 6  D 6 ) spectrum revealed the presence of the hydroxyl group at carbon 4. Exact mass 141.0550 (calc. 141.0552) for C 7  H 9  O 3  showed the cleavage between carbon 4 and carbon 5; ms of the TMS derivative of 1 and the dihydro derivative also supported the structure of fragment A. ##STR6## 
     The structure of fragment B, which contains the two tetrahydrofuran rings and the remaining two hydroxyl groups, was elucidated by essentially the same techniques. 
       13  C Nmr (50 MHz) and  1  H nmr resonances were directly analogous to similar signals of asimicin [Rupprecht et al., Heterocycles, 24: 1197-1201 (1986)], uvaricin [Jolad et al., J. Org. Chem., 47: 3151-3153 (1982)], and rolliniastatin [Pettit et al., Can. J. Chem., 65: 1433-1435 (1987)], indicating the common presence of a bistetrahydrofuran moiety as illustrated in fragment B. Exact mass measurement of a second peak at m/z 141.0909 (calc. 141.0916) corresponded to C 8  H 13  O 2 . It is proposed that this peak represents cleavage between carbons 15 and 16, and between carbons 23 and 24. Eims of the TMS and acetyl derivatives of 1,  1  H nmr of the triacetate of 1 and  1  H-- 1  H decoupling experiments on the 470 MHz spectrometer for 1 confirmed these assignments. ##STR7## 
     Subtracting fragments A and B, the remainder, C 20  H 41 , of the structure of bullatacin (1) belongs to the unsubstituted alkyl chain. This was corroborated by multiple CH 2  resonances between δ 1.2-1.5 in the  1  H nmr (C 6  D 6 ) and δ 20-40 in the  13  C nmr (Tables 2 and 3). The placement of fragments A and B along the hydrocarbon chain was accomplished through analysis of the ms fragmentation pattern of 1 and its TMS, acetate, and hydrogenation derivatives. 
     The absolute value two dimensional homonuclear correlated spectrum (2D-COSY, 200 MHz) of bullatacin (1) confirmed the proton assignments (Table 2) and proton connectivities except for the aliphatic --CH 2  -- chain. 
     Surprisingly, this carbon skeleton of bullatacin (1) is the same as that of asimicin [Rupprecht et al., Heterocycles, 24: 1197-1201, (1986)], and rolliniastatin [Pettit et al. Can. J. Chem., 65: 1433-1435, (1987) . However, the mp, co-TLC,  1  H nmr, and most importantly, the bioactivities are different. The compounds are stereoisomers at one or more of their eight chiral centers. Since 1 yielded crystals which were not suitable for X-ray diffraction studies and since the acetogenins are difficult to convert into crystalline derivatives suitable for X-ray analysis [Pettit et al., Can. J. Chem., 65: 1433-1435, (1987)], other methods were used to predict the stereochemistry. 
     First, the relative configuration of six of the eight chiral centers, those on the bistetrahydrofuran ring system and its two adjacent hydroxyl bearing carbons, was obtained by comparing the  1  H nmr (CDCl 3 ) spectral signals of bullatacin (1) acetate (Table 4) with those of a recently published series of synthetic dibutylated diacetate bistetrahydrofuran models and uvaricin and uvaricin acetate; stereochemical information could then be extracted from &#34;iterative and synergistic&#34; analysis of very small differences in the high-field proton chemical shifts [Hoye et al. J. Am. Chem. Soc., 109: 4402-4403 (1987)]. 
     
                                           TABLE 4__________________________________________________________________________.sup.1 H NMR Assignments and Comparisons of Acetate Derivatives.*Bullatacin     Bullatacinone                     Asimicin  Uvaricintriacetate     diacetate  triacetate                               acetate(470 MHz, CDCl.sub.3)          (470 MHz, CDCl.sub.3)                     300 MHz, CDCl.sub.3)                               (300 MHz, CDCl.sub.3)__________________________________________________________________________2      --      3.02ddd 2, 3a(12.82)                      --        --          2, 3b(9.34)          2, 35a(9.34)          2, 35b(3.42)3a   2.52dddd  1.74m      2.53      2.26ddd3b   2.58ddt   2.00m      2.534    5.11dddd 4, 3a(4.0)          4.53dddd   5.09      1.25m4, 3b(7.0)4, 5a(10.1)4, 5b(1.8)5    1.5-2.0   1.5-2.0    1.5-1.9   1.25m6-13 1.25br s  1.25br s   1.25      1.25m14   1.5-2.0   1.5-2.0    1.5-1.9   1.75-1.915   4.88dt 15, 16(8.0)          4.80dt 15, 16(8.0)                     4.85      4.86ddd15, 14(2.0)          15, 14(2.0)16   4.00ddd   4.00ddd    3.98      3.98ddd17, 181.5-2.0   1.5-2.0    1.5-1.9   1.75-1.919, 203.9br t   3.9br t    3.9       3.89br, t21, 221.5-2.0   1.5-2.0    1.5-1.9   1.75-1.923   4.00ddd   4.00ddd    2.98      3.98ddd24   4.93ddd 24, 25a(3.0)          4.93ddd 24, 25a(3.0)                     3.83      4.92ddd24, 25b(2.8)          24, 25b(2.8)25   1.5-2.0   1.5-2.0    1.5-1.9   1.75-1.926-331.28br s  1.28br s   1.25      1.25m34   0.89t 34, 33(7.05)          0.89t 34, 33(7.05)                     0.87      0.878t35   7.09d 35, 36(1.29)          2.66dd 35a, 35b(18.3)                     7.06      6.98qq          35a, 2(9.34)          3.04dd 35b, 2(3.42)36   5.02qq     --        5.01      4.99dtq37   1.41d 36, 37(7.05)          2.20s      1.42      1.40d4 OAc2.04s      --        2.01       --15 OAc2.06s     2.05s      2.06      2.074s24 OAc2.09s     2.08s      2.06      2.046s__________________________________________________________________________ *Preparation: 10 mg of parent compound is dissolved in a 1:1 mixture of acetic anhydride/pyridine and left overnight. Addition of ice water followed by extraction with chloroform yields the corresponding peracetylated derivative. 
    
     Results of bullatacin acetate nmr resonances (Table 4) suggested that the configuration of fragment B is threo, trans, threo, trans, erythro, the same as in uvaricin or erythro, trans, threo, trans, and threo. However, differences in the  1  H nmr (CDCl 3 , 200 MHz) of bullatacin compared with that of uvaricin indicate the probable configuration of bullatacin to be erythro, trans, threo, trans, threo, as illustrated for 1. Similarly, we have determined that asimicin is threo, trans, threo, trans, threo. From X-ray data, rolliniastatin is reported to be threo, cis, threo, cis, and erythro [Pettit et al., Can. J. Chem., 65: 1433-1435, (1987)]. 
     The stereochemistry at the remaining two chiral centers, carbon 4 and carbon 36, was determined by comparing nmr spectral data with those in the literature for rolliniastatin [Pettit et al., Can. J. Chem., 65: 1433-1435, (1987)]. Furthermore, essentially identical CD curves for rolliniastatin, asimicin and bullatacin suggested their stereochemical identity in this region. The CD data are given below. 
     Bullatacin (1) (c, 0.025, abs. EtOH); [θ] 265 , 0.00°; [θ] 260 , -298.56°; [θ] 250 , -995.20 °, [θ] 240 , -2189.44°; [θ] 233 , -2587.52°; [θ] 230 , -2348.67°; [θ] 225 , 0.00°; [θ] 220 , 4378.88°; [θ] 218 , -7961.60°. 
     Rolliniastatin (c, 0.025; abs. EtOH); [θ] 265 , 0.00°; [θ] 260 , -199.04°; [θ] 250 , -1393.28°; [θ] 240 , -2587.52°; [θ] 235 , -2786.56°; [θ] 230 , -2089.92°; [θ] 225 , 0.00°; and [θ] 220 , 6369,28°. 
     Asimicin (c, 0.025; abs. EtOH); [θ] 265 , 0.00°; [θ] 260 , -199.04°; [θ] 250 , -995.20°; [θ] 246 , -2288.95°; [θ] 234 , -2687.04°; [θ] 230 , -2388.48°; [θ] 225 , -59.712°; [θ] 224 , 0.00°; [θ] 220 , 4080.32°; and [θ] 218 , 7862.08°. 
     From the above data, we conclude that the structure of bullatacin, with stereochemistry defined, is as illustrated as in formula 1 above. 
     B. Structural Characterization of Bullatacinone (2) 
     Mp 90.5-90.7°; [α]23°/589=+12.00, [α]23°/578=+12.50, [α]23°/546=+14.50, [α]23°/436=+29.75, [α]23°/365=+51.25, (c, 0.400, CHCl 3 ); cims (NH 3 ) m/z 623 (MH + ) and 640 (MNH +   4 ); cims (isobutane) m/z 623 (MH + ) and 661 (MH+38); hr cims 623.4839 (calc. 623.4889) for C 37  H 66  O 7  ; eims;  1  H nmr (Table 2);  13  C nmr (Table 3). 
     Negative results with Kedde&#39;s reagent, weak uv end absorption at λ max  203.5 nm (ε=3799), and ir (KBr) absorptions at 1770 and 1715 cm -1  showed an absence of the conjugated α,β-unsaturated λ-lactone ring usually found in these acetogenins. 
     The  1  H nmr (Table 2) of bullatacinone (2) looked quite similar to that of 1 with an obvious presence of two tetrahydrofuran rings with adjacent hydroxyls as in fragment B of 1. To determine the stereochemistry of fragment B of 2, the diacetate of 2 was made, and the  1  H nmr (470 MHz) (CDCl 3 ) spectrum was compared with that of the triacetate of 1 (Table 4). The comparison showed that the bistetrahydrofuran ring of 2, together with the two adjacent hydroxyl groups at carbon 15 and carbon 24, have exactly the same configuration as that of 1, i.e., erythro, trans, threo, trans, threo. The  13  C nmr of 2 (Table 3) showed two carboxyl carbons and the absence of the vinyl carbon seen in 1. 
     The eims and cims of bullatacinone (2) and the 2-TMS derivative and exact mass measurements of selected fragments showed that the alkyl chain was the same length as in 1. The observation that the 2-TMS derivative in cims did not show the m/z 213 typical of the A fragment of 1 suggested the lack of a 4-OH. The ir spectrum of 2 at 1770 and 1715 cm -1  suggested two carbonyl groups of which one corresponds to a saturated lactone ring. In the  1  H nmr (470 MHz) of 2-acetate, a characteristic signal at δ2.20 (s, 3H) in CDCl 3  suggested a terminal methyl ketone. It seemed likely that this compound has the lactone formed with the 4-OH, leaving a carbonyl at C36. To test this proposition, 1 was converted into 2 by treating with base to hydrolyze the lactone and to recyclize upon acidification: 
     15 milligrams of 1 was treated with 2% KOH in t--BuOH (1 ml) at room temperature for 24 hours; the solution was acidified with 10% HCl to pH 1-2, set aside for 30 minutes, and, partitioned between CHCl 3  /H 2  O. The reaction product (in the CHCl 3  residue) showed two components (TLC), and one was identical to 2 (four TLC systems). Resolution on a microcolumn of Si gel gave 2 which was identical (co-TLC, cims and  1  H nmr) with 2 isolated from the plant material. 
     To determine the absolute stereochemistry at C4, the CD curve of bullatacinone (shown below) was compared with those of rubrenolide and rubrynolide, two 2,4-disubstituted-γ-butyrolactones whose structures are shown below [Franca et al., Phytochemistry, 16: 257-262, (1977)]. ##STR8## CD of bullatacinone (2): (c, 0.1, abs. EtOH); [θ] 250 , 0.00°; [θ] 240 , -124.4°; [θ] 235 , -248.8°; [θ] 230 , -497.6°; [θ] 225 , -870.8°; [θ] 220 , -1555°; [θ] 215 , -1990.4°; [θ] 212 , -2177°; [θ] 210 , -1492.8°; [θ] 207 , -746.4°; [θ] 205 , -0.00°. 2 showed a negative Cotton effect, whereas rubrenolide and rubrynolide gave positive Cotton effects. Application of the modified Hudson lactone rule [Klyne et al., J. Chem. Soc., 7237-7242, (1965)] enabled the assignment of the S-configuration to the chiral center at C4. For the stereochemistry at position 2, the  13  C nmr showed three carbons (C1, C2 and C4) as doublets. This suggested that bullatacinone is a mixture of two stereoisomers at carbon 2.  1  H Nmr confirmed this proposal. From the  13  C nmr, the ratio of the two isomers at C2 was estimated to be 2:1. The phase sensitive two dimensional homonuclear correlated spectrum (2D COSY, 500 MHz) of bullatacinone confirmed the proton assignments and provided the proton proton connectivities in Table 2. From the above data, we conclude that bullatacinone is as illustrated in structure 2 partially racemized at carbon 2. 
     Biological Activity 
     A. Anticancer Activities 
     Anticancer activity is a potential use even for the crude extract. The bioassay results for the lethality of brine shrimp (BST), the inhibition of crown gall tumors on potato discs (PD), the reversal in morphology of db-cAMP induced AC glioma cells from that of mature, differentiated astrocytes to that of immature AC glioma cells (mouse brain cells) (9ASK), the dose that inhibits cell growth to one-half that of untreated poorly differentiated human epidermic carcinoma (9KB), the dose that inhibits cell growth to one-half that of a methylcholanthrene-induced lymphoid neoplasm in a DBA/2 mouse (9PS), and the percent displacement in a protein kinase C test for initial extract fractions are shown in Table 1. Obviously, the bioactivity is concentrated in F005. 
     Corresponding to these bioactivities, the pure compounds isolated from F005, bullatacin and bullatacinone, showed additional promising results (Table 5). 
     
                                           TABLE 5__________________________________________________________________________Bioactivities of 1 and 2 and their derivatives  BST  LC.sub.50, mcg/ml  95%  Confidence          9PS     9KB     A549    HT29  Interval          ED.sub.50, mcg/ml                  ED.sub.50 mcg/ml                          ED.sub.50, mcg/ml                                  ED.sub.50, mcg/ml__________________________________________________________________________Bullatacin(1)  0.00159 10.sup.-15 -10.sup.-16                  6.188 × 10.sup.-14                          1.25 × 10.sup.-13                                   10.sup.-12  0.0124  ↓  0.0008Bullatacin  5.7     3.89 × 10.sup.-3                  6.85 × 10.sup.-7                          2 × 10.sup.-3                                  &gt;10.sup.-1.sup.triacetate  17.12(1-Ac) ↓   2.84Dihydro-.sup.b  0.0145    --.sup.a                    --    &lt;10.sup.-6                                  3.33 × 10.sup.-5bullatacin  0.0300(1-H.sub.2)  ↓  0.0100Bullatacinone  0.0030  4.23 × 10.sup.-3                  &lt;10.sup.-12                           10.sup.-3                                  5 × 10.sup.-12(2)    0.0090  ↓  0.0000Bullatacinone  &gt;10     4.22 × 10.sup.-2                  5 × 10.sup.3                          2.8 × 10.sup.-2                                   10.sup.-1diacetate(2-Ac)__________________________________________________________________________ .sup.a A dash () indicates that tests were not conducted. .sup.b Obtained by hydrogenation of bullatacin in the presence of a Pd/C catalyst following standard laboratory procedures. 
    
     These two compounds are the most bioactive acetogenins reported to this date. When derivatized, such as by acetylation and hydrogenation, the bioactivities decrease as shown in Table 5. But the safety range for treatments might be larger. In this table, two cytotoxicity tests against human tumor cell lines such as the human lung carcinoma (A-549) [Giard et al., J. Nat. Cancer Inst. 51: 1417-1423 (1973)] and the human colon adenocarcinoma (HT-29) [Fogh, J. (ed.) Human Tumor Cells, In Vitro, pp. 115-159, Plenum Press, New York (1975)] have been performed. The fact that bullatacin is active to both these cell lines to 10 -12  -10 -13  mcg/ml while bullatacinone is 5×10 -12  mcg/ml to human colon adenocarcinoma and 10 -3  mcg/ml to human lung carcinoma suggested that bullatacinone has more selective cytotoxicities than bullatacin. Only a 7% displacement in a protein kinase C test at a concentration of 10 μM proved that bullatacin is not a phorbol ligand. A negative result in the protein tyrosine kinase test excluded its possible mechanism of action on protein tyrosine kinase. The mode of action for the acetogenins is still unknown, but it is postulated to involve cell membranes. 
     To test further the selective specificity of antitumor activities, bullatacin was sent to the NIH, NCI at Bethesda, Md., for their human tumor cell line panel tests including leukemia, non-small cell lung cancer, small cell lung cancer, colon cancer, breast cancer, CNS cancer melanoma, ovarian cancer, and renal cancer. These cytotoxicity results are shown in Table 6, where IC50 is the concentration of the compound that was found to cause 50% inhibition of the growth of each cell line listed under &#34;Cell&#34;. IC90 is the concentration of compound that was found to cause 90% inhibition of the growth of each cell line. From the results, we can say that bullatacin is best active for certain CNS cancers, non-small cell lung cancers, leukemias, and ovarian cancers. 
     The substantially pure compounds in accordance with this invention can be formulated into dosage forms using pharmaceutically acceptable carriers for oral or parenteral administration to patients in need of oncolytic therapy. Preferred dose levels will depend on the attending physician&#39;s assessment of both the nature of the patient&#39;s particular cancerous condition and the overall physical condition of the patient. Effective antitumor doses of the present compounds may range from about 1 microgram per kilogram to about 200 micrograms per kilogram of patient body weight, more preferably between about 2 micrograms to about 100 micrograms per kilogram of patient body weight. 
     
                       TABLE 6______________________________________NCI Developmental Therapeutical ProgramIn Vitro Testing Results for bullatacin (1)DISEASE  CELL LINE   IC.sub.50 (μg/ml)                            IC.sub.90 (μg/ml______________________________________Leukemia HOLT-4      1.41 × 10°                            4.72 × 10°    HL-60 TB    &lt;9.26 × 10.sup.-4                              --    K562        &lt;9.26 × 10.sup.-4                            4.5 × 10°    P388/ADR    &lt;9.26 × 10.sup.-4                            2.56 × 10°    CCRF-CEM    8.94 × 10.sup.-5                            6.94 × 10.sup.-2    P388        &gt;9.24 × 10.sup.-2                            &gt;9.24 × 10.sup.-2Non-Small    H522        2.46 × 10°                            &gt;9.24 × 10°Cell Lung    H125        2.40 × 10°                            &gt;9.26 × 10°Cancer   H23         2.38 × 10°                            9.21 × 10°    H460        2.87 × 10.sup.-5                            1.85 × 10.sup.-4    H322        &lt;9.26 × 10.sup.-4                            &gt;9.26 × 10°    EKV-X       1.15 × 10.sup.-4                            &gt;9.26 ×  10.sup.-2    HOP-62      &lt;9.26 × 10.sup.-4                            5.13 × 10°    SK-MES-1    &gt;9.24 × 10.sup.-2                            &gt;9.24 × 10.sup.-2    A-549(ATCC) 5.04 × 10                            &gt;9.24 × 10.sup.-2Small Cell    H82         1.99 × 10°                            7.14 × 10°Lung Cancer    H524        2.69 × 10°                            7.54 × 10°    H69         2.44 × 10°                            7.52 × 10°    H146        6.75 × 10                            &gt;9.26 × 10°Colon Cancer    SW620       5.64 × 10.sup.-3                            5.66 × 10°    LOVO        9.15 × 10.sup.-4                            4.69 × 10°    DLD-1       2.76 × 10°                            8.62 × 10°    HCC-2998    4.05 × 10°                            &gt;9.26 × 10    HT29        3.84 × 10.sup.-3                            &gt;9.24 × 10.sup.-2Breast   MCF7        &gt;9.24 × 10.sup.-2                            &gt;9.24 × 10.sup.-2CancerCNS Cancer    TE-671      2.56 × 10.sup.-5                            2.74 × 10.sup.-3    U251        &lt;9.24 ×  10.sup.-6                            4.37 × 10.sup.-5    SNB-19      &gt;9.26 × 10.sup.-1                            &gt;9.26 × 10.sup.-1    SNB-44      4.42 × 10°                            &gt;9.26 × 10°    SNB-75      3.35 × 10°                            &gt;9.26 × 10°Melanoma SK-MEL5     &gt;9.26 × 10.sup.-4                            5.22 × 10°    RPMI-7951   2.73 × 10°                            7.71 × 10°    MALME-3M    2.82 × 10°                            7.67 × 10°    LOX         &lt;9.26 × 10.sup.-4                            3.76 × 10°    SK-MEL2     3.25 × 10                            8.64 × 10°Ovarian  A2780       3.77 × 10.sup.-5                            6.50 × 10.sup.-4Cancer   OVCAR-8     2.57 × 10°                            7.65 × 10°    OVCAR-5     8.80 × 10°                            &gt;9.26 × 10°    OVCAR-4     &gt;9.24 × 10.sup.-2                            &gt;9.24 × 10.sup.-2    OVCAR-3     &gt;9.24 × 10.sup.-2                            &gt;9.24 × 10.sup.-2Renal Cancer    A498        4.24 × 10.sup.-5                            3.71 × 10.sup.- 3    A704        1.36 × 10°                            8.00 × 10°    SN12-K1     2.24 × 10.sup.-5                            2.87 × 10.sup.-4    UO-31       1.94 × 10°                            &gt;9.26 × 10°    CAKI-1      3.94 × 10.sup.-3                            &gt;9.24 × 10.sup.-2______________________________________ 
    
     B. Pesticidal Activity 
     Pesticidal bioassays on initial fractions were conducted at Eli Lilly Laboratories (Greenfield, Ind.) following standard procedures with seven indicator organisms: mosquito larvae (ML), blow fly larvae (BFL), corn root worm (CRW), two-spotted spider mite (2SSM), southern army worm (SAW), melon aphid (MA), and Haemonchus contortus (HC). Results in Table 7 showed unusually high activity for F005 on ML, 2SSM, MA, and BFL. This suggested that F005, itself could be used as a potent pesticidal agent. 
     The pesticidal activities of bullatacin (1) and bullatacinone (2) are shown in Table 7 A. The results indicate that bullatacin is significantly toxic to cotton aphids (CA) at 1 ppm, southern corn rootworm (CRW) at 24 ppm, and two-spotted spider mites (2SSM) at 10 ppm. The compounds can be formulated at concentrations of about 1 to about 200 ppm in conventional liquid or solid forms for application to pest infected areas. The lack of pesticidal activity for bullatacinone indicates that it did not contribute to the pesticidal activities of the initial ethanol extract. 
     It is understood that the foregoing detailed description is given merely by way of illustration and that modification and variation may be made therein without departing from the spirit and scope of the invention. 
     
                                           TABLE 7__________________________________________________________________________Pesticidal activities of inital fractions                     MISPLEX (% MORTALITY)        (% MORTALITY)ML              BFL              HC CRW SAW 2SSM                             MA  ACTIVITY100 ppm 10 ppm       1 ppm           1% 0.1%                 300 5000                         5000                             5000                                PLEX                                    MIS__________________________________________________________________________F001   100  100 100 100              0  0   0    0  0  A   NF002    0    0   0   0 0  0   0    0   0 A   NF003   100  100 100 100              0  0   0   90  90 A   AF004   100   0   0   0 0  0   0    0   0 A   NF005   100  100 100 100              0  0   0   80  80 A   AF006   100   0   0   0 0  0   0   70  80 A   A__________________________________________________________________________ 
    
     
                       TABLE 7A______________________________________Pesticidal activities of 1 and 2..sup.a  Pest/percent controlCompound ppm    CA      ML   SAW    CRW   2SSM______________________________________Bullatacin(1)    0.5    --.sup.b                    0   --     --    --     1     80       0   --     --    --     6     --      --   --     20    --     10    80      80   0      --    20     24    --      --   --     80    --    100    80      --   0      --    30    400    90      --   0      --    20Bullatacinone    0.5    --       0   --     --    --(2)       1     --       0   --     --    --     6     --      --   --      0    --     10     0       0   0      --     0     24    --      --   --      0    --    100     0      --   0      --     0    400     0      --   0      --     0______________________________________ .sup.a See pesticidal activity section for details and definitions of abbreviation. .sup.b A dash () indicates that tests were not conducted.