Abstract:
An improved circulatory packed bed reactor includes a fermentor, a ventilation system communicating with the fermentor through pipes, and a circulatory system. The fermentor has an upper pipe joint and a lower pipe joint. The circulatory system includes a pipe device and a cell-fixation device. The pipe device includes a circulating pipe outside the fermentor and circulating liquid pumps installed on the circulating pipe. The circulating pipe has one end connecting the upper pipe joint of the fermentor and communicating with the fermentor and another end connecting the lower pipe joint of the fermentor and communicating with the fermentor. The cell-fixation device is located in the fermentor. The cell-fixation device in the fermentor makes the cells in stationary state and submerge in the fermentation to perform deep submerged fermentation, which enhances the density of the fermented cells and cell&#39;s tolerance to toxicity, increases the output and the content of cell&#39;s secretions, and enables the cells re-utilization.

Description:
BACKGROUND OF THE INVENTION 
       [0001]    1. Field of the Invention 
         [0002]    The present invention relates to fermentation engineering field and, more particularly, to an improved circulatory packed bed reactor. 
         [0003]    2. The Related Art 
         [0004]    In microbial engineering, fungal mycelium is usually utilized to be fermented to produce various medicines or health products, such as proteins, polysaccharides, antibiotics, amino acids, etc. Conventional submerged fermentation method is that culture fluid is fermented with free cells. Such method has many disadvantages, for example, the cell density of the liquid fermentation is low, the speed of fermentation reaction is slow, the cell&#39;s tolerance to toxicity is poor, and the output and the content of cell production are low. What&#39;s more, the cells can not be reused. On the other hand, during liquid fermentation, the fermentation liquid can not contact with fresh air fully and uniformly, so the fermentation liquid is poor at dissolve of oxygen. Moreover, the heat produced by fermentation of cell can not be quickly removed, which will cause cells autolysis. Accordingly, the result of fermentation is undesirable. 
       BRIEF SUMMARY OF THE INVENTION 
       [0005]    Accordingly, one aspect of the present invention is to provide an improved circulatory packed bed reactor which can make cells fixation in its fermentor, enhance cell density and cell&#39;s tolerance to toxicity, increase output and content of cell&#39;s secretions, and enable cells re-utilization. 
         [0006]    Another aspect of the present invention is to provide an improved circulatory packed bed reactor with inner and outer double circulatory systems which make the fermentation liquid always in circulating state and contact with fresh air fully and uniformly, therefore, the fermentation liquid has a good ability of dissolve of oxygen, and the output and content of cell secretions is increased. 
         [0007]    Another aspect of the present invention is to provide an improved circulatory packed bed reactor that can do fermentation repeatedly until the content of the target production in the fermentation liquid meet requirement. 
         [0008]    Still another aspect of the present invention is to provide an improved circulatory packed reactor that is advantage in low energy consumption, low cost, and easy operation. 
         [0009]    To attain the above objects, the present invention provides an improved circulatory packed bed reactor including a fermentor, a ventilation system communicating with the fermentor through pipes, and a circulatory system. The fermentor has an upper pipe joint and a lower pipe joint. The circulatory system includes a pipe device and a cell-fixation device. The pipe device includes a circulating pipe outside the fermentor and circulating liquid pumps installed on the circulating pipe. The circulating pipe has one end connecting the upper pipe joint of the fermentor and communicating with the fermentor and another end connecting the lower pipe joint of the fermentor and communicating with the fermentor. The cell-fixation device is located in the fermentor. 
         [0010]    Preferably, the circulating pipe also includes a liquid-product tube with one end thereof joining and communicating with the circulating pipe and another end thereof serving as a liquid-product exit. A first reversing valve is disposed at the joint of the liquid-product tube and the circulating pipe. 
         [0011]    Preferably, the circulating pipe also includes a supplement liquid pipeline with one end there of joining and communicating with the circulating pipe and another end thereof serving as a supplement liquid entrance. A second reversing valve is disposed at the joint of the supplement liquid pipeline and the circulating pipe. 
         [0012]    Preferably, the circulatory packed bed reactor further includes a second valve, a cell interceptor and a sampling port mounted on the circulating pipe and located between the lower pipe joint of the fermentor and the circulating liquid pumps in turn. 
         [0013]    Preferably, the cell-fixation device includes a centre shaft and at least one layer of spokes mounted on the centre shaft and extanding radially towards outward from the centre shaft. A plurality of cell-fixation carries is mounted on the spokes. 
         [0014]    The cell-fixation carries are porous medium. 
         [0015]    The fermentor includes a top lid and a side wall. An adjusting speed motor is installed on the outer surface of the top lid. The top end of the centre shaft passes through the top lid of the fermentor and is installed on the adjusting speed motor so that the whole cell-fixation device hangs in the fermentor. At least one runner pipe is installed on the inner surface of the side wall and communicates with the circulating pipe. At least one atomizing spray head is installed on and communicates with the circulating pipe. Liquid in the circulating pipes passes through the runner pipe and the atomizing spray heads and is sprayed to the cell-fixation device by the atomizing spray heads. 
         [0016]    Preferably, the circulatory packed bed reactor also includes an autoclave that communicates with the fermentor, the circulatory system and the ventilation system respectively. The autoclave includes an outer wall, an interlayer, an inner wall and an inner chamber. The outer wall and the inner wall are joined together at the top and bottom of the autoclave, respectively. A channel is disposed at the top joint. The autoclave communicates with the upper pipe joint of the fermentor through the channel so as to communicate with the inner chamber of the fermentor. The interlayer is filled with water. The bottom of the autoclave defines an outlet that communicates with the circulating pipe. A venturi tube is installed in the inner chamber of the autoclave. One end of the venture tube opens to the bottom of the autoclave, and the other end of the venture tube passes through the inner wall and the outer wall of the autoclave to communicate with the ventilation system. 
         [0017]    Preferably, the channel has a fourth valve mounted thereon whose switch controls the communication and isolation between the fermentor and the autoclave. The channel defines a channel pipe joint between the lower pipe joint of the fermentor and the fourth valve. The channel pipe joint joins and communicates with the circulating pipes, and a third reversing valve is disposed at the joint of the channel pipe joint and the circulating pipe. 
         [0018]    Preferably, the circulatory packed bed reactor further includes a ventilation pipe connecting the fermentor and the inner chamber of the autoclave. A sixth valve and a seventh valve are mounted on the ventilation pipe. The circulatory packed bed reactor also includes a pressure detecting device communicating with the interlayer of the autoclave and the ventilation pipe at a point between the sixth valve and the seventh valve, respectively. 
         [0019]    The advantages of the present invention are as follows: (1) the cell-fixation device in the fermentor makes the cells in stationary state and submerge in the fermentation to perform deep submerged fermentation, which enhances the density of the fermented cells and cell&#39;s tolerance to toxicity and increases the output and the content of cell&#39;s secretions, what&#39;s more, the fixed cells can be reused. (2) The double circulatory systems make the fermented liquid circulation in the fermentor, that&#39;s to say, one part of the fermented liquid is used to yield new production constantly, another part of the fermented liquid is mixed with fresh culture fluid, then the mixture is fed in the reactor at a constant speed. Thus the reatctor can not only continue producing but also improve the content of production in the fermented liquid. Furthermore, the culture being fed in the reactor makes the fixed cells grow under flow condition, which improves the transfer of heat and the content of dissolved oxygen in the reactor which in turn, benefits to cell growth and propagation and biosynthesis of production. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0020]    The present invention will be apparent to those skilled in the art by reading the following description of preferred embodiments thereof, with reference to the attached drawings, in which: 
           [0021]      FIG. 1  is a schematic diagram of an improved circulatory packed bed reactor of a first embodiment in accordance with the present invention; 
           [0022]      FIG. 2  is a top view of a cell-fixation device of the improved circulatory packed bed reactor with a lid thereof removed; 
           [0023]      FIG. 3  is a schematic diagram of the improved circulatory packed bed reactor of a second embodiment in accordance with the present invention; 
           [0024]      FIG. 4  illustrates the circular flow direction of the cell stock solution in the improved circulatory packed bed reactor of the first embodiment shown in  FIG. 1 ; 
           [0025]      FIG. 5  illustrates the circular flow direction of the culture fluid or the mixture of the culture fluid and the fermentation liquid in the improved circulatory packed bed reactor of the first embodiment shown in  FIG. 1 ; 
           [0026]      FIG. 6  illustrates the flow direction of the finished product in the improved circulatory packed bed reactor of the first embodiment shown in  FIG. 1 ; 
           [0027]      FIG. 7  illustrates the flow direction of the culture fluid in the improved circulatory packed bed reactor of the second embodiment shown in  FIG. 3 ; 
           [0028]      FIG. 8  illustrates the circular flow direction of the cell stock solution in the circulatory improved packed bed reactor of the second embodiment shown in  FIG. 3 ; 
           [0029]      FIG. 9  illustrates the circular flow direction of the culture fluid or the mixture of the culture fluid and the fermentation liquid in the improved circulatory packed bed reactor of the second embodiment shown in  FIG. 3 ; and 
           [0030]      FIG. 10  illustrates the flow direction of the finished product in the improved circulatory packed bed reactor of the second embodiment shown in  FIG. 3 . 
       
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 
       [0031]    For facilitating understanding, like components are designated by like reference numerals throughout the various embodiments of the invention as shown in the attached drawings. 
         [0032]      FIG. 1  illustrates an improved circulatory packed bed reactor of a first embodiment in accordance with the present invention, including a fermentor  100 , a ventilation system  200 , and a circulatory system  300 . 
         [0033]    The fermentor  100  includes a top lid  110 , a side wall  120 , a bottom  130  and a inner chamber  140 . An adjustable speed motor  111 , a breather valve  112  and a sight glass light  113  are installed on the outer surface of the top lid  110 . A first valve  1121  is disposed on the breather valve  112  and controls the open and close of the breather valve  112 . A pressure detecting device  121  and two sight glasses  122  are installed on the outer surface of the side wall  120 . One or several runner pipes  123  are installed on the inner surface of the side wall  120  and communicate with the circulatory system  300 . One or several anodize spay heads  124  are installed on the runner pipes  123  and communicates with the circulatory system  300 . A ventilation outlet  125  is installed on the sidewall  120  and communicates with the ventilation system  200 . A pipe outlet  126  is installed on the upper of the fermentor and communicates with the circulatory system  300 . A lower pipe joint  131  is installed on the bottom of fermentor  130  and also communicates with the circulatory system  300 . 
         [0034]    The circulatory system  300  includes a cell-fixation device  310  and a pipe device  320 . Referring to  FIG. 2  in conjunction with  FIG. 1 , the cell-fixation device  310  includes a centre shaft  311  and a plurality of spokes  312  mounted on the centre shaft  311  and extending radially towards outward from the central of the centre shaft  311 . A plurality of cell-fixation carries  313 , which are porous medium, are mounted on the spokes  312 . The centre shaft  311  passes through the top lid  110  of the fermentor and is installed on the adjustable speed motor  111 , thus the whole cell-fixation device  310  hangs in the fermentor The adjustable speed motor  111  controls the turning speed of the cell-fixation device  310 . The anodize spay heads  124  spay culture fluid or fermented liquid from the circulatory system  300  to the cell-fixation device  310 . 
         [0035]    The pipe device  320  includes a circulating pipe  321  outside the fermentor and circulating liquid pumps  322  installed on the circulating pipe  321 . The circulating pipe  321  has one end connecting the upper pipe joint  126  of the fermentor  100  and communicating with the fermentor  100 , and another end connecting the lower pipe joint  131  of the fermentor  100  and communicating with the fermentor  100 . 
         [0036]    The circulating pipe  321  also includes a liquid-product pipeline  325  with one end thereof joining and communicating with the circulating pipe  321  and another end thereof serving as a liquid-product exit  326 . A first reversing valve  323  is disposed at the joint of the liquid-product exit  326  and the circulating pipe  321 . 
         [0037]    The circulating pipe  321  also includes a supplement liquid pipeline  327  with one end thereof joining and communicating with the circulating pipe  321  and another end thereof serving as a supplement liquid entrance  328 . A second reversing valve  324  is disposed at the joint of the supplement liquid entrance  328  and circulating pipe  321 . 
         [0038]    It is appreciated that, alternately, the supplement liquid entrance  328  can also be disposed directly on the upper part of the fermentor  100 , the liquid-product exit  326  is disposed directly on the lower part of the fermentor  100 , or the supplement liquid entrance  328  is disposed directly on the upper part of the fermentor  100 , the liquid-product exit  326  communicates with the circulating pipe  321  through the liquid-product pipeline  325  and a valve  323  is disposed at the joint, or the liquid-product exit  326  is disposed at the lower part of the fermentor  100 , the supplement liquid entrance  328  communicates with the circulating pipe  321  through the supplement liquid pipeline  327 , and a second reversing valve  324  is disposed at the joint. 
         [0039]    A second valve  329 , a cell interceptor  330 , and a sampling port  331  are mounted on the circulating pipe  321 . The second valve  329 , the cell interceptor  330 , and the sampling port  331  are located between the lower pipe joint  131  under the fermentor  100  and the second reversing valve  324  in turn. 
         [0040]    The ventilation system  200  includes a roots blower  201 , an air filtrating system  202 , an air cool system  203 , a ventilation pipe  204 , and a third valve  205  on the ventilation pipe  204 . The roots blower  201 , the air filtrating system  202 , and the air cool system  203  communicate with each other through pipe in turn. The ventilation pipe  204  communicates with the ventilation outlet  125  on the side wall of the fermentor  100 , and air is transferred to the fermentor  100  through the ventilation pipe  204 . 
         [0041]      FIG. 3  illustrates an improved circulatory packed bed reactor of a second embodiment in accordance with the present invention. The reactor includes an autoclave  400  communicating with the fermentor  100 , the ventilation system  200 , and the circulating pipe  300 , respectively. The autoclave  400  includes an outer wall  410 , an interlay  420 , an inner wall  430 , and an inner chamber  440 . The outer wall  410  and the inner wall  430  are joined together at the top and the bottom of the autoclave  400 , respectively. A channel  411  is disposed between the top joint and the lower pipe joint  131  at the bottom  130  of the fermentor  100  so that the inner chamber  440  of the autoclave  400  communicates with the inner chamber  140  of the fermentor  100  through the channel  411 . A fourth valve  412  is disposed on the channel  411 . The opening and closing of the fourth valve  412  control the communication and isolation of the inner chamber  140  of the fermentor  100  and the inner chamber  440  of the autoclave  400 . A channel pipeline joint  413  is disposed on the channel  411  and is located between the down pipe joint  131  of the fermentor  100  and the fourth valve  412 . The channel pipeline joint  413  communicates with the supplement liquid pipeline  327  through circulating pipe  321  and passes through the second valve  332 . At the bottom of the autoclave  400  has an outlet  414 . The outlet  414  communicates with the bottom of the circulating pipe  312 . A venturi tube  431  is installed in the inner chamber  440  of the autoclave  400 . One end of the venturi tube  431  opens to the bottom of the autoclave  400 , the other end of venturi tube  431  passes through the inner wall and the outer wall of the autoclave  100  to communicate with the ventilation system  200 . A pressure detecting device  432  is installed on the inner wall  430  of the autoclave  400  to detect the pressure of the inner chamber  440 . 
         [0042]    On the ventilation pipeline  206  has a fifth valve  207  which controls whether transfer air to the inner chamber  440  of the autoclave  400  or not. The ventilation pipeline  206  communicates with the ventilation pipeline  204  between the air cool system  203  and the third valve  205 . 
         [0043]    The interlay  420  communicates with the inner chamber  140  of the fermentor  100  through a ventilation pipeline  421 . A sixth valve  423  and a seventh valve  424  are disposed on the ventilation pipeline  421 . A pressure detecting device  425  communicates with the interlay  420  and the ventilation pipeline  421  between the sixth valve  423  and the seventh valve  424 , respectively, and detects the pressure in the interlay  420  of the autoclave  400 . The autoclave  400  also has an electric device  433 . The interlay  420  is filled with water. The electric device  433  heats the water in the interlay  420  and sterilizes the inner chamber  440  of the autoclave  400 . When the sixth valve  423  and the seventh valve  424  on the ventilation pipeline  421  are opening, the inner chamber  140  of the fermentor  100  is also sterilized. When the sixth valve  423  is closed while the seventh valve  424  is opening, only the inner chamber  440  is sterilized. The autoclave  400  also has a pressure detecting device  432 , a temperature detecting device  434 , a pH detecting device  435  and a dissolved oxygen detecting device  436 , all of those serve to detect the parameters of the fermented liquid in the inner chamber  440 . 
         [0044]    The working process of the present circulatory packed bed reactor will be described in great detail hereinafter: 
         [0045]    The working process of the first embodiment of the circulatory packed reactor: 
         [0046]    1. Sterilization: 
         [0047]    Open the top lid  110  of the fermentor  100 ; install the cell-fixation carries  313  on the spokes  312  of the cell-fixation device  310 ; close the top lid  110 ; and sterilize the reactor. 
         [0048]    2. Inoculation: 
         [0049]    Referring to  FIG. 4  in which the arrows show the flow direction of cell stock solution. 
         [0050]    When the reactor is cooled at room temperature, input the prepared cell stock solution into the supplement liquid pipeline  328  through the supplement liquid entrance  327 ; rotate the second reversing valve  324 ; cause the cell stock solution to flow towards the cycle liquid bump  322  by the second valve  324 ; with the power provided by the cycle liquid bump  322 , the cell stock solution flows toward the first reversing valve  323  through circulating pipe; rotate the first reversing valve  323 ; cause the cell stock solution to flow towards the runner pipeline  123  in the fermentor  100  through pipeline and through the runner pipeline  123  to the anodize spay heads  124 ; the anodize spay heads  124  spay the cell stock solution to the cell-fixation carries  313  on which the cell is fixed. The cell stock solution gathers at the bottom  130  of the fermentor, passes through the lower pipe joint  131  in the bottom  130  of the fermentor into the circulating pipe  321  again, then passes through the second valve  329 , the cell interceptor  330 , the sampling port  331  and the second reversing valve  324  in turn, and finally enters another circulation. The cell stock solution flows circularly and causes an amount of cells fixation on the cell-fixation carries  313 . When the amount of fixed cells achieves the predetermined value, stop the incubation. 
         [0051]    In the process, the flow direction of the cell stock solution is as follow: the supplement liquid entrance  328 —the supplement liquid pipeline  327 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the runner pipeline  123 —the anodize spay heads  124 —the cell-fixation carries  313 —the lower pipe joint  131  in the bottom  130  of the fermentor—the second valve  329 —the cell interceptor  330 —the sampling port  331 —the second reversing valve  324 —the cycle liquid bump  322   
         [0052]    3. The First Fermentation Incubation: 
         [0053]    Referring to  FIG. 5  in which the arrows show the flow direction of the culture fluid. 
         [0054]    After the culture fluid is sterilized, input the culture fluid into the supplement liquid pipeline  327  through the supplement liquid entrance  328 ; rotate the second reversing valve  324 ; cause the culture fluid to flow towards the cycle liquid bump  322  through the second reversing valve  324 ; with the power provided by the cycle liquid bump  322 , the culture fluid flows towards the first reversing valve  323  through circulating pipe; rotate the first reversing valve  323 ; cause the cell stock solution to flow towards the runner pipeline  123  in the fermentor  100  through pipeline and through the runner pipeline  123  to the anodize spay heads  124 ; the anodize spay heads  124  spay the cell stock solution to the cell-fixation carries  313  on which the cell is fixed. The culture fluid gathers at the bottom  130  of the fermentor, passes through the lower pipe joint  131  in the bottom  130  of the fermentor into circulating pipe  321  again, then passes through the second valve  329 , the cell interceptor  330 , the sampling port  331  and the second reversing valve  324  in turn, and finally enters another circulation. 
         [0055]    The flow direction of the culture fluid is as follow: the supplement liquid entrance  328 —the supplement liquid pipeline  327 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the runner pipeline  123 —the anodize spay heads  124 —the cell-fixation carries  313 —the lower pipe joint  131  in the bottom  130  of the fermentor—the second valve  329 —the cell interceptor  330 —the sampling port  331 —the second reversing valve  324   
         [0056]    4. Circulatory Fermentation and Incubation and Collection Part of Production at the Same Time: 
         [0057]    Referring to  FIG. 6  in which the arrows show the flow direction of the production. 
         [0058]    Collect production: when the cells are fermented for some time, take up a little sample from the sampling port  331 ; detect the content in the fermented liquid. If the content attains the target value, stop the first circulatory fermentation. Then rotate the first reversing valve  323  to lead the fermented liquid to flow towards the liquid-product entrance  326 , and collect the production. 
         [0059]    The flow direction of the liquid-product is as follow: the lower pipe joint  131 —the second valve  329 —the cell interceptor  330 —the sampling port  331 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the liquid-product pipeline  325 —the liquid-product entrance  326 . 
         [0060]    Circulatory fermentation: Please refer back to  FIG. 5  in which the arrows show the flow direction of the mixture liquid. While collecting the production, retain parts of the fermented liquid in the fermentor  100 ; supplement fresh culture into the fermentor; after the fresh culture is sterilized, input the fresh culture into the supplement liquid pipeline  327  through the supplement liquid entrance  328 ; rotate the second reversing valve  324 ; lead the culture to flow towards the cycle liquid bump  322  through the second reversing valve  324 ; and with the power provided by the cycle liquid bump  322 , the culture flow towards the first reversing valve  323  through circulating pipe; rotate the first reversing valve  323 ; lead the culture to flow towards the runner pipeline  123  in the fermentor  100  through pipeline and through the runner pipeline  123  to the anodize spay heads  124 ; the anodize spay heads  124  spay the culture to the cell-fixation carries  313 . The culture fluid gathers at the bottom  130  of the fermentor and mixes with the retain fermenting liquid. The mixture passes through the lower pipe joint  131  in the bottom  130  of the fermentor into circulating pipe  321  again, then passes through the second valve  329 , the cell interceptor  330 , the sampling port  331  and the second reversing valve  324  in turn, and finally enters another circulation. 
         [0061]    The flow direction of the mixture is as follow: the culture fluid—the supplement liquid entrance  328 —the supplement liquid pipeline  327 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the runner pipeline  123 —the anodize spay heads  124 —the cell-fixation carries  313 —the lower pipe joint  131  in the bottom  130  of the fermentor—the second valve  329 —the cell interceptor  330 —the sampling port  331 —the second reversing valve  324 . 
         [0062]    Repeat the steps 3 and 4, until the content in the fermented liquid attains target value. 
         [0063]    5. Collect the Production: Collect the Production and Stop Fermentation. 
         [0064]    It is noted that in the whole process of the cell&#39;s growth and fermentation, the ventilation system feeds air through the pipeline into the fermentor  100  for the cell&#39;s growth and discharges the exhaust gas through the breather valve all the time. 
         [0065]    The working process of the second embodiment of the circulatory packed bed reactor: 
         [0066]    1. Sterilization: 
         [0067]    Referring to  FIG. 7  in which the arrows show the flow direction of the culture. 
         [0068]    Open the top lid  110  of fermentor  100 ; install cell-fixation carries  313  on the radius  312  of cell-fixation device  310 ; close the top lid  110 ; open the valve  423  and the valve  424  on the ventilation pipeline  421 ; make the interlay  420  of the autoclave communicate with the inner chamber  140  of the fermentor; input the prepared culture into the supplement liquid pipeline  327  through the supplement liquid entrance  328 ; rotate the second reversing valve  324  and the third reversing valve  332 ; open the second valve  329 ; lead the culture to flow into the circulating pipe through the third reversing valve and flow towards the sampling port  331  through the second reversing valve  324 . The culture passes through the sampling port  331  and the cell interceptor  330 , reaches the outlet  414  at the bottom of the fermentor, and enters the inner chamber  440  of the autoclave  400 . Then turn on the electric device  450  to sterilize the inner chamber  140  of the fermentor  100 , the inner chamber  440  of the autoclave  400 , the cell-fixation carries  313  in the inner chamber  140 , and the culture. 
         [0069]    The flow direction of the culture fluid: the supplement liquid entrance  328 —the supplement liquid pipeline  327 —the third reversing valve  332 —the second reversing valve  324 —the sampling port  331 —the cell interceptor  330 —the second valve  329 —the outlet  414  at the bottom of the fermentor  100 —the inner chamber  440  of the autoclave  400 . 
         [0070]    2. Inoculation: 
         [0071]    Referring to  FIG. 8  in which the arrows show the flow direction of cell stock solution. 
         [0072]    When the reactor is cooled at room temperature, close the valve  423  and the valve  424  on the ventilation pipeline  421 ; make the inner chamber  140  of the fermentor  100  isolate with the interlay  430  and the inner chamber  440  of the autoclave  100 ; input the prepared cell stock solution into the supplement liquid pipeline  327  through the supplement liquid entrance  328 ; rotate the third reversing valve  332  and the second reversing valve  324 ; lead the cell stock solution to flow towards the cycle liquid bump  322  through the third valve  332  and the second valve  324 ; with the power provided by the cycle liquid bump  322 , the cell stock solution flows toward the first reversing valve  323  through circulating pipe; rotate the first reversing valve  323 ; lead the cell stock solution to flow towards the runner pipeline  123  in the fermentor  100  through pipeline and through the runner pipeline  123  to the anodize spay heads  124 ; the anodize spay heads  124  spay the cell stock solution to the cell-fixation carries  313  on which the cell is fixed. The cell stock solution gathers at the bottom  130  of the fermentor  100 , passes through the channel  411  to flow towards the third reversing valve  332 , and finally enters another circulation. The cell stock solution flows circularly to cause an amount of cells fixation on the cell-fixation carries  313 . When the amount of cell inoculation attains the predetermined value, stop the circulatory. 
         [0073]    The flow direction of the cell stock solution is as follow: the supplement liquid entrance  328 —the supplement liquid pipeline  327 —the third reversing valve  332 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the circulating pipeline  123 —the anodize spay heads  124 —the cell-fixation carries  313 —the bottom  130  of the fermentor  100 —the channel  411 —the third reversing valve  332 . 
         [0074]    3. The First Time Fermentation and Incubation: 
         [0075]    Referring to  FIG. 9  in which the arrows show the flow direction of the culture fluid. 
         [0076]    When the culture is cooled at room temperature, prepare for fermentation. Open the second valve  329  and the valve  412  on the channel  411 ; the culture flows out from the outlet  414  at the bottom  414  of the autoclave, passes through the second valve  329 , the cell interceptor  330  and the sampling port  331  in turn, and attains the second reversing valve  324 ; rotate the second reversing valve  324 ; cause the culture fluid to flow towards the cycle liquid bump  322  through the pipelines; with the power provided by the cycle liquid bump  322 , the culture fluid flow towards the first reversing valve  323  through circulating pipe; rotate the first reversing valve  323 ; lead the culture fluid to flow towards the circulating pipeline  123  in the fermentor  100  through pipeline and through the runner pipeline  123  to the anodize spay heads  124 ; the anodize spay heads  124  spay the culture fluid to the cell-fixation carries  313 . The culture fluid gathers at the bottom  130  of the fermentor, and passes through the channel  411  into the inner chamber  140  of the autoclave  400 , then passes through the outlet  414  at the bottom of the autoclave  400 , and finally enters another circulation. For prevent the cell from choking the outlet  414  in the circulatory, open the valve  207  and transfer air to the venturi tube  332  through the ventilation pipeline  206 , which causes the liquid in the inner chamber  430  to roll, thus avoid the outlet  414  to be choked. 
         [0077]    The flow direction of the fermented liquid: the culture fluid—the outlet  414 —the second valve  329 —the cell interceptor  330 —the sampling port  331 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the runner pipeline  123 —the anodize spay heads  124 —the cell-fixation carries  313 —the bottom  130  of the fermentor  100 —the channel  411 —the inner chamber  440  of the autoclave  400 —the outlet  414 . 
         [0078]    4. The Second Time Circulatory Fermentation and Incubation: 
         [0079]    Referring to  FIG. 10  in which the arrows show the flow direction of the production. 
         [0080]    Collect the production: when the cells are fermented for some time, take up a litter sample from the sampling port  331  and detect the content in the fermented liquid. If the content attains the target value, stop the first circulatory fermentation. Then close the valve  412  on the channel  411 ; isolate the inner chamber  140  of the fermentor  100  and the inner chamber  440  of the autoclave  400 ; rotate the first reversing valve  323 ; lead the fermented liquid to flow towards the production outlet  326 ; and collect the production. 
         [0081]    The flow direction of the production: the production—the outlet  414 —the second valve  329 —the cell interceptor  330 —the sampling port  331 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the production pipelines  325 —the production outlet  326 . 
         [0082]    circulatory fermentation: please also refer to  FIG. 9  in which the arrows show the flow direction of the mixture. Retain part of the fermented liquid; rotate the first reversing valve  323 ; lead the fermented liquid to flow towards the fermentor  100  and store at the fermentor  100 . When the fermented liquid in the inner chamber  440  of the autoclave  440  flows out completely, rotate the third reversing valve  332  and the second reversing valve  324 ; input the prepared culture fluid into the supplement outlet  328 ; lead the culture fluid to flow towards the cell interceptor  330  through the third reversing valve  332  and the second reversing valve  324  and into the inner chamber  440  of the autoclave  400  and sterilize the culture fluid; cool the culture fluid to room temperature; open the valve  412  on the channel  411 ; the retained fermented liquid is mixed with the culture fluid; rotate the second valve  324  and the first valve  323 ; the mixture passes through the outlet  414  of the autoclave, the cell interceptor  330 , the sampling port  331 , the second reversing valve  324 , the cycle liquid bump  322 , and the first reversing valve  323 , flows into the runner pipeline  123  in the fermentor  100  through pipeline and through the runner pipeline  123 , and reaches to the anodize spay heads  124 ; the anodize spay heads  124  spay the culture on the cell-fixation carries; and finally enters another circulation. 
         [0083]    The flow direction of the mixture is as follow: in the autoclave, the retained fermented liquid in the fermentor is mixed with the culture fluid in the autoclave—the outlet  414 —the second valve  329 —the cell interceptor  330 —the sampling port  331 —the second reversing valve  324 —the cycle liquid bump  322 —the first reversing valve  323 —the runner pipeline  123 —the anodize spay heads  124 —the cell—fixation carries  313 —the channel  441 —the inner chamber  140  of autoclave  400 —the outlet  414 . 
         [0084]    Repeat the steps 3 and 4, until the content in the fermented liquid attains target value. 
         [0085]    5. Collect the Production: Collect the Production and Stop Fermentation. 
         [0086]    It is noted that in the whole process of the cell&#39;s growth and fermentation, the ventilation system feeds air through the pipeline into the fermentor  100  for the cell&#39;s growth and discharges the exhaust gas through the breather valve all the time. 
         [0087]    The foregoing description of the present invention has been presented for purposes of illustration and description. It is not intended to be exhaustive or limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. Such modifications and variations that may be apparent to those skilled in the art are intended to be included within the scope of this invention as defined by the accompanying claims.