Abstract:
The present invention provides novel radiation associated markers. The radiation associated markers may be one or more of albumin, LTGF-β, or any protein or peptide listed in any one of Tables 1, 2, 3, 4, 5, and 6 provided herein. The present invention also provides methods of assessing exposure to ionizing radiation by determining the presence of one or more radiation associated markers. The methods may optionally include quantifying one or more of the radiation associated markers. The methods may further include comparing the amount of one or more radiation associated markers in the sample determined to be present in the sample with either (i) the amount determined for temporally matched, normal samples or (ii) the amount determined for samples obtained from individuals or subjects that have not been exposed to an elevated level of ionizing radiation. The present invention further provides a method of predicting outcome in a subject after exposure to elevated levels of ionizing radiation. Further, the present invention provides a method of determining the amount of radiation therapy that has been delivered to a particular tissue. Also, the present invention provides kits for assessing the likelihood of significant damage, death, illness or medical complications post exposure to elevated levels of ionizing radiation by determining the presence or absence or by quantifying the amount of one or more radiation associated markers.

Description:
FIELD OF THE INVENTION 
     This invention relates to new protein biomarkers that may be present after exposure to ionizing radiation and methods of assessing exposure to ionizing radiation as well as diagnostic tests and kits for evaluating exposure to ionizing radiation. 
     BACKGROUND OF THE INVENTION 
     Recent work has identified a transcription factor, nuclear factor KAPPA B (NF-KB) which induces the TNF-α encoding gene and activates the cyclooxygenase-2 (COX-2) pathway. At 24 hours post irradiation HIF-1a and COX-2 protein levels were increased. In addition to its well established DNA-damage effects, ionizing radiation induces cell death, and radiation-induced activation of acid sphingomyelinases (ASMases) and the generation of ceramide. Ceramide is generated from sphingomyeline by the action of a neutral or ASMase or by de novo synthesis coordinated through the enzyme ceramidesynthase. Once generated, ceramide may serve as a second messenger molecule in signaling responses to physiologic or environmental stimuli, or it may be converted to a variety of structural or effector molecules. With a single dose of 3 Gy, there is activation of protein kinase B/AKT (PKB/AKT) signaling. Within minutes of irradiation, phosphorylation of the serine/threonin protein kinase PKB/AKT at serine-residue 473 appears. This activation of PKB/AKT contributes to inhibit glycogen synthase kinase-3beta (GSK3beta), which has a clear inhibitory role in endothelial cell survival. 
     One problem first responders face in the event of a nuclear disaster is to rapidly and efficiently identify victims that need medical treatment and determine what level of treatment is appropriate. It is a priority to minimize time to diagnosis to identify patient treatment needs, minimize the time required for emergency technicians (ET) provide diagnosis, minimize the number of interactions with the patient, minimize system stockpile cost, minimize the dependence on infrastructure (communications, transport, etc.), minimize the needs for trained personnel to administer tests, and uniquely identify patient and diagnostic results. 
     Simple, quick test strips that change color, like the ones used for sugar or albumin by diabetics, are currently available. They are used without medical or other highly trained personal or expensive equipment. It would be highly desirable to provide such a simple test strip that changes color that could be used as evidence of ionizing radiation (IR) exposure. A positive test that could be used to identify a person who has been exposed to radioactive material has many applications. 
     SUMMARY OF THE INVENTION 
     In a first aspect, the present invention provides novel radiation associated markers. The radiation associated markers may be one or more of albumin, LTGF-β, or any protein or peptide listed in any one of Tables 1, 2, 3, 4, 5, and 6 provided herein. 
     In a second aspect, the present invention provides methods of assessing exposure to ionizing radiation by determining the presence of one or more radiation associated markers. The radiation associated markers may be one or more of albumin, LTGF-β, or any protein or peptide listed in any one of Tables 1, 2, 3, 4, 5, and 6 provided herein. 
     The sample used for determining the presence of one or more radiation associated markers may be, for instance, saliva, a buccal swab, amniotic fluid, plasma, serum, urine or blood. The one or more radiation associated markers may be identified by many methods well known to those of skill in the art including Matrix assisted laser desorption ionization (MALDI) mass spectrometry. The radiation associated markers may also be identified by contacting the sample with an antibody which specifically binds to the radiation associated marker under conditions permitting formation of a complex between the antibody and the radiation associated marker, and optionally measuring the amount of complexes formed, thereby determining the amount of the radiation associated marker. 
     The methods may optionally include quantifying one or more of the radiation associated markers. The methods may further include comparing the amount of one or more radiation associated markers in the sample determined to be present in the sample with either (i) the amount determined for temporally matched, normal samples or (ii) the amount determined for samples obtained from individuals or subjects that have not been exposed to an elevated level of ionizing radiation. The relative absence of one or more of the radiation associated markers in the sample indicates that the individual has not been exposed to an elevated level of ionizing radiation, and the relative abundance of one or more of the radiation associated markers in the sample indicates that the individual has been exposed to an elevated level of ionizing radiation. In some embodiments, one or more of the radiation associated markers are not present in detectable levels in normal tissue unaffected by elevated levels of ionizing radiation at all. In other embodiments, one or more of the radiation associated markers is present in levels that are 10%, 20%, 30%, 50%, 75% or more greater than the amount of the corresponding radiation associated marker in normal tissue unaffected by elevated levels of ionizing radiation at all. In still other embodiments, one or more of the radiation associated markers is present in levels that are 2×, 3×, 4×, 5×, 10×, 25×, 50×, 100×, 1,000×, or even 10,000× or more greater than the amount of the corresponding radiation associated marker in normal tissue unaffected by elevated levels of ionizing radiation at all. 
     In some embodiments, the elevated levels of ionizing radiation may be 10%, 20%, 30%, 50%, 75% or more greater than the amount of ionizing radiation experienced normally from environmental sources. In still other embodiments, the elevated levels of ionizing radiation may be 2×, 3×, 4×, 5×, 10×, 25×, 50×, 100×, 500×, 1,000×, 5,000×, 10,000×100,000×, 1,000,000× or more greater than the amount of ionizing radiation experienced normally from environmental sources. 
     The methods of the present invention are applicable to determining the relative amount of ionizing radiation to which an individual has been exposed. Likewise, the methods of the present invention are applicable to determining the relative amount of damage caused by ionizing radiation to an individual. The relative amount of ionizing radiation to which an individual has been exposed and the relative amount of damage caused by ionizing radiation to an individual may be determined, for instance, by quantifying the amount of one or more radiation associated marker present in a tissue or by determining the presence of one or more radiation associated marker that is not present in a tissue until a particular threshold of ionizing radiation is experience or until a particular threshold of damage to a tissue is crossed. 
     In a third aspect, the present invention further provides a method of predicting outcome in a subject after exposure to elevated levels of ionizing radiation. In some embodiments, it is a method of predicting the likelihood of surviving long term after exposure to elevated ionizing radiation. In other embodiments, it is a method of predicting the relative amount of damage caused to an individual or to a tissue by exposure to elevated ionizing radiation comprising determining the presence or the relative presence as compared to normal samples or normal subjects of one or more radiation associated markers. One, two, three, or more of the radiation associated markers may be present in the sample and may be identified. One, two, three, or more of the radiation associated markers may be present in the sample and may be elevated in comparison to samples obtained from individuals that have not been exposed to elevated levels of ionizing radiation. 
     The sample used for determining the presence of one or more radiation associated markers may be, for instance, saliva, plasma, serum, urine or blood. The one or more radiation associated markers may be identified by many methods well known to those of skill in the art including Matrix assisted laser desorption ionization (MALDI) mass spectrometry. The radiation associated markers may also be identified by contacting the sample with an antibody which specifically binds to the radiation associated marker under conditions permitting formation of a complex between the antibody and the radiation associated marker, and optionally measuring the amount of complexes formed, thereby determining the amount of the radiation associated marker in the sample. 
     The methods may optionally include quantifying one or more of the radiation associated markers. The methods may further include comparing the amount of radiation associated markers in the sample determined to be present in the sample with either (i) the amount determined for temporally matched, normal samples or (ii) the amount determined for samples obtained from individuals that have not been exposed to elevated levels of ionizing radiation. The relative absence of one or more of the radiation associated markers in the sample indicates that the likelihood of significant damage, death, illness or medical complications is relatively low, and the relative abundance of one or more of the radiation associated markers in the sample indicates that the likelihood of significant damage, death, illness or medical complications is relatively high. 
     The invention provides methods for determining the relative likelihood of significant damage, death, illness or medical complications comprising the steps of: (a) obtaining a biological sample from an individual; (b) measuring an amount of one or more radiation associated markers present in the biological sample; and (c) comparing the amount of one or more radiation associated markers with a predetermined value, whereby the amount of radiation associated markers relative to the predetermined value indicates the likelihood of significant damage, death, illness or medical complications or the relative likelihood of a positive or negative medical outcome. 
     The methods of the invention can be used alone or in combination with any known test for determining the relative likelihood of significant damage, death, illness or medical complications or for determining the prognosis of exposure to ionizing radiation, including, but not limited to, X-Ray, ultrasound, CAT scan, and other blood marker analysis. The methods of the invention may be used to screen a biological sample collected at any time either before or after a first exposure to elevated levels of ionizing radiation has occurred. 
     In a fourth aspect, the present invention further provides a method of determining the amount of radiation therapy that has been delivered to a particular tissue. In other embodiments, it is a method of predicting the relative amount of damage caused to a particular tissue by exposure to elevated ionizing radiation from radiation therapy comprising determining the presence or the relative presence as compared to normal samples or normal subjects of one or more radiation associated markers. One, two, three, or more of the radiation associated markers may be present in the tissue and may be identified. One, two, three, or more of the radiation associated markers may be present in the tissue and may be elevated in comparison to tissues that have not received radiation therapy. 
     In a fifth aspect, the present invention provides a kit for assessing the likelihood of significant damage, death, illness or medical complications post exposure to elevated levels of ionizing radiation by determining the presence or absence or by quantifying the amount of one or more radiation associated markers. The kits may contain biomarker identification and bioassay systems that address both the problem of a very rapid and inexpensive Point of Care (POC) diagnostic determination of radiation exposure and of quantitative High Throughput (HT) diagnostic determination of radiation exposure. The kits and bioassay systems are useful as a means for making a rapid diagnosis and a quantitative high throughput diagnosis. The kits and bioassay systems utilize biochemistry that serves as the basis for assay systems based on one or more biomarkers indicative of a dose or ionizing radiation received. Immunoassays may be provided to quantitatively measure radiation dose. These immunoassays may be incorporated into commercial diagnostic systems using both an established strip test technology to provide a &gt;2 Gy screening diagnosis for quick triage and microfluidics technology that provides a low cost high throughput quantitation capability to indicate dosing level at +/−0.5 Gy to determine the course of treatment to be received. Examples of such microfluidics devices include a handheld microfluidic reader available from Claros Diagnostics, Inc. 
     The kits and bioassays use identified biomarkers. Commercial antibodies may be obtained or immunoassays developed to provide a quantitative measure of the biomarkers. The commercial antibodies or immunoassays may be embedded into a test strip using established technology. These test strips may provide, for instance, a 0-5 or 0-2 Gy quantitative screening diagnosis for quick triage. In some embodiments, the test strips may change color to provide a useful gauge of the amount of elevated ionizing radiation to which a tissue or an individual has been exposed or to gauge the amount of damage a tissue or an individual has experienced. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1  demonstrates the presence of radiation associated biomarkers post exposure to elevated levels of ionizing radiation. 
         FIG. 2  demonstrates the presence of radiation associated biomarkers post exposure to elevated levels of ionizing radiation. 
         FIG. 3  represents a MALDI spectrum, linear mode, control, 15 minutes post 3Gy, and 30 minute post 3 Gy. Note the protein peaks 63870.32 Da at 15 minutes, and 72449.25 at 30 minutes. These proteins can be tentatively identified with bioinformatics in the NCBInr database. The tip of the 63870.32 peak is superimposed on the bottom of the 30 minute spectrum. 
         FIG. 4  represents MALDI, reflectron mode, control, bottom; 15 minutes post 3 Gy, center; and 30 minute post 3 Gy, top. Note 955.87 Da and 1184.12 Da peptides that appear at 15 minutes post 3Gy, and the 939.07 Da peptide that appears 30 minute post 3 Gy. 
         FIG. 5  represents a MALDI image of murine tongue one hour post 1 Gy TBI, longitudinal section. Note peripheral foci of Albumin (Red in image). 
         FIG. 6  depicts the histopathology of murine tongue one hour post 1 Gy TBI, longitudinal section. Note minimal destructive changes in the epithelial cornified spicule layer and edema in the basal region. 
         FIG. 7  represents a MALDI image. Note the marked increase in peripheral foci of Albumin compared to the post 1 Gy TBI image (Red in image). 
         FIG. 8  depicts the histopathology of murine tongue one hour post 2 Gy TBI, longitudinal section. Note progressive destructive changes in the epithelial layer with disruption of the cornified spicule layer, increased scattered chromatin debris and edema of the sub-basement membrane layer. 
         FIG. 9  represents a MALDI image of murine tongue one hour post 3 Gy TBI, longitudinal section. Note loss of peripheral foci of albumin consistant with destructive changes in the epithelial layer and increase in central foci of Albumin compared to the post 2 Gy image. 
         FIG. 10  depicts the histopathology of murine tongue one hour post 3 Gy TBI, longitudinal section. Note progressive destructive changes and virtual complete loss of the cornified spicule layer corresponding to the loss of peripheral albumin foci (peripheral zone) in the MALDI image, increased scattered chromatin debris, and increased edema of the sub-basement membrane layer. 
         FIG. 11  represents the LAP monomer, light blue in the image. 
         FIG. 12  represents the LAP monomer, light blue and red in the image. 
         FIG. 13  represents the LAP monomer, light blue and red in the image. 
         FIG. 14  represents the Da Hemoglobin Subunit α, and Parathymosinare only found one hour post 1 Gy IR, red in the image. 
         FIG. 15  depicts a fatty acid-binding protein adipocyte seen 1 hour post 1 and 2 Gy IR, red in the image. 
         FIG. 16  demonstrates one hour post 2 Gy. Triosphosphate isomerase and superoxide dismutase are seen one hour post 2 Gy, but not seen in normal tissue or at one hour post 1 Gy. Red in the image. 
         FIG. 17  demonstrates hemaglobin α chains and BC011074 NID seen one hour post 3 Gy. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     As used herein, the following terms mean as follows: 
     As used herein, “radiation associated marker” means any molecule, such as a protein, peptide or fragment thereof whose presence, absence or amount in absolute quantity or in quantity relative to other molecules may be used as evidence of exposure to elevated levels of ionizing radiation. Stated differently, a radiation associated marker may be any molecule that may be used as a statistically significant predictor of exposure to elevated levels of ionizing radiation, or that may used as a statistically significant predictor of the amount of ionizing radiation to which an individual has been exposed, or that may be used as a statistically significant predictor or clinical outcome post exposure to elevated levels of ionizing radiation. 
     As used herein, a “predetermined value” is a standardized value based on a control. For example, a predetermined value can be based on an amount of radiation associated marker that is present in a biological sample obtained from an individual. 
     The term “amount” is used within the context of the analytical method used to measure the different radiation associated markers and may reflect a number, a concentration, etc., depending upon the analytical method chosen to measure the radiation associated markers. 
     The term “biological sample,” as used herein, generally refers to urine, saliva, serum, plasma, tears, or amniotic fluid. Buccal mucosa is an especially useful “biological sample” for the present invention because of its known sensitivity to ionizing radiation and easy accessibility for study. 
     The term “detecting” as used herein refers to identifying the presence of, identifying the presence of in relative amounts relative to another molecule or radiation associated marker relative to a predetermined value, or quantifying in absolute amounts. 
     The term “assessing exposure to ionizing radiation” includes quantifying the amount of ionizing radiation to which an individual has been exposed, quantifying the length of time to which an individual has been exposed to ionizing radiation, estimating or quantifying the amount of damage to a tissue or to an individual after exposure to ionizing radiation, and providing a prognosis of likely clinical sequelae or survival probability. 
     The term “elevated levels of ionizing radiation” encompasses instances where the ionizing radiation is present in amounts that are 10%, 20%, 30%, 50%, 75% or more greater than the amount of ionizing radiation experienced normally from environmental sources. The term “elevated levels of ionizing radiation” also encompasses instances where the ionizing radiation is present in amounts that are 2×, 3×, 4×, 5×, 10×, 25×, 50×, 100×, 500×, 1,000×, 5,000×, 10,000× 100,000×, 1,000,000× or more greater than the amount of ionizing radiation experienced normally from environmental sources. Ionizing radiation may be quantified and expressed in terms of a gray (Gy). One gray is the absorption of one joule of energy, in the form of ionizing radiation, by one kilogram of matter. For X-rays and gamma rays, these are the same units as the sievert (Sv). To avoid any risk of confusion between the absorbed dose and the equivalent dose, one must use the corresponding special units, namely the gray instead of the joule per kilogram for absorbed dose and the sievert instead of the joule per kilogram for the dose equivalent. The gray measures the deposited energy of radiation. The biological effects vary by the type and energy of the radiation and the organism and tissues involved. The sievert attempts to account for these variations. A whole-body exposure to 5 or more grays of high-energy radiation at one time usually leads to death within 14 days. This dosage represents 375 joules for a 75 kg adult (equivalent to the chemical energy in 20 mg of sugar). Since grays are such large amounts of radiation, medical use of radiation is typically measured in milligrays (mGy). The average radiation dose from an abdominal x-ray is 1.4 mGy, that from an abdominal CT scan is 8.0 mGy, that from a pelvic CT scan is 25 mGy, and that from a selective spiral CT scan of the abdomen and the pelvis is 30 mGy. One gray is equivalent to 100 rad. Therefore, the term “elevated levels of ionizing radiation” may be defined in some instances to mean a radiation dose greater than 0.25, 0.50, 1.0, 1.5, 2.0, 5.0, 10.0, 20.0, 50.0 or 100 mGy. In other instances, the term “elevated levels of ionizing radiation” may be defined to mean a radiation dose greater than 0.25, 0.50, 1.0, 1.5, 2.0, or 5.0 Gy. 
     According to an embodiment of this invention, the sample may be a saliva sample or a buccal swab. The sample may be obtained, for instance, about 15 minutes, 30 minutes, 1 hour, 2 hours, 6 hours, 12 hours, 1 day, 2 days or a week after exposure to elevated levels of ionizing radiation. In a particular embodiment of this invention, the sample is a buccal swab. 
     In addition, the present invention provides a method for determining the amount of radiation associated marker of in a sample comprising: (a) contacting the sample with an antibody which specifically binds to a radiation associated marker under conditions permitting formation of a complex between the antibody and the radiation associated marker; and (b) determining the amount of complexes formed thereby determining the amount of radiation associated marker in the sample. 
     This invention is illustrated in the experimental details section which follows. These sections are set forth to aid in an understanding of the invention but are not intended to, and should not be construed to, limit in any way the invention as set forth in the claims which follow thereafter. 
     Radiation associated markers can be directly measured, for example, using anti-marker antibodies in an immunoassay, such as a Western blot or ELISA. Radiation associated markers can be indirectly measured, for example, using a capture antibody that binds the radiation associated marker. 
     The amount of radiation associated markers in a biological sample can be determined using any method known in the art, including, but not limited to, immunoassays using antibodies specific for the radiation associated marker. Any assay that functions to qualitatively or quantitatively determine variations in sample concentrations of radiation associated markers from normal levels can be employed in the practice of the invention. 
     For example, a monoclonal anti-radiation associated marker antibody can be generated by immunizing a mouse with the radiation associated marker. Once an immune response is detected, e.g., antibodies specific for the radiation associated marker are detected in the mouse serum, the mouse spleen is harvested and splenocytes are isolated. The splenocytes are then fused by well-known techniques to any suitable myeloma cells, for example, cells from cell line SP20 available from the American Type Culture Collection (ATCC). Hybridomas are selected and cloned by limited dilution. The hybridoma clones are then assayed by methods known in the art for cells that secrete antibodies capable of binding the radiation associated marker. Ascites fluid, which generally contains high levels of antibodies, can be generated by immunizing mice with positive hybridoma clones. 
     Any type of fusion phage, monoclonal, or polyclonal antibodies can be used in immunoassays of the invention, so long as the antibodies can be used in a reproducible fashion as markers for various radiation associated markers or as measures of the different levels of radiation associated markers observed in normal and variant populations. 
     In one embodiment, an amount of radiation associated marker can be measured using a capture antibody followed by a labeled secondary antibody using a strategy as described, for example, in U.S. Pat. No. 6,429,018, hereby incorporated by reference. The label on the secondary antibody can comprise any chemical, radioactive, lanthanide, colored dye, or genetic tag used in enzyme-linked immunosorbent assays (ELISAs), Western blots, and other sensitive and specific immunoassays and immunoradiometric assays using known methodology. These include conjugating the antibody with horseradish peroxidase or alkaline phosphatase that are easily measurable, typically using colorimetric, fluorometric or luminescent substrates. Genetic labels include firefly luciferase, employed because luciferase produces a bioluminescent molecule when incubated with its substrate, luciferin. 
     Serum albumin, by test strip, and numerous other novel protein and peptide biomarkers of ionizing radiation (IR) are consistently found by mass spectrometry in ex vivo wiping and histology sections of murine tongue epithelium one hour post 1, 2, and 3 Gy. Albumin and other proteins are not seen in non-irradiated tissue. Keratin is a ubiquitous component of tongue tissue, and can be seen at all levels of IR. The additional proteins in nonirradiated tissue, 1, 2, and 3 Gy are shown in the LCMS mass spectrometry Mascot search engine results. These biomarkers can be identified at POC with a simple oral cavity swab.  FIG. 1 . These biomarkers can be confirmed hours to days after the event with the same mass spectrometry techniques used in the initial biomarker identification with similar samples. 
     Matrix-Assisted Laser Desorption/Ionization (MALDI). 
     Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is most similar in character to electrospray ionization both in relative softness and the ions produced (although it causes many fewer multiply charged ions). The ionization is triggered by a laser beam (normally a nitrogen laser). A matrix is used to protect the biomolecule from being destroyed by direct laser beam and to facilitate vaporization and ionization. 
     The matrix consists of crystallized molecules, of which the three most commonly used are 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid), α-cyano-4-hydroxycinnamic acid (alpha-cyano or alpha-matrix) and 2,5-dihydroxybenzoic acid (DHB). A solution of one of these molecules is made, often in a mixture of highly purified water and an organic solvent (normally acetonitrile (ACN) or ethanol). Trifluoroacetic acid (TFA) may also be added. A good example of a matrix-solution would be 20 mg/mL sinapinic acid in ACN:water:TFA (50:50:0.1). The identity of suitable matrix compounds is determined to some extent by trial and error, but they are based on some specific molecular design considerations: they have a low molecular weight; they are acidic and act as a proton source to encourage ionization of the analyte; they have a strong optical absorption in the UV and efficiently absorb the laser irradiation; and they are functionalized with polar groups allowing use in aqueous solutions. 
     The matrix solution is mixed with the analyte (e.g. protein sample). The organic solvent allows hydrophobic molecules to dissolve into the solution, while the water allows for water-soluble (hydrophilic) molecules to do the same. This solution is spotted onto a MALDI plate (usually a metal plate designed for this purpose). The solvents vaporize, leaving only the recrystallized matrix, but now with analyte molecules spread throughout the crystals. The matrix and the analyte are said to be co-crystallized in a MALDI spot. 
     The laser is fired at the crystals in the MALDI spot. The matrix absorbs the laser energy, and the matrix is ionized by this event. The matrix transfers part of its charge to the analyte molecules (e.g. protein) thereby ionizing them while still protecting them from the disruptive energy of the laser. Ions observed after this process consist of a neutral molecule [M] and an added or removed ion. Together, they form a quasimolecular ion, for example [M+H] +  in the case of an added proton, [M+Na] +  in the case of an added sodium ion, or [M−H] −  in the case of a removed proton. MALDI is capable of creating singly-charged ions, but multiply charged ions ([M+nH] n+ ) can also be created, as a function of the matrix, the laser intensity and/or the voltage used. Note that these are all even-electron species. Ion signals of radical cations can be observed eg. in case of matrix molecules and other stable molecules. 
     Atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) is an ionization technique (ion source) that in contrast to vacuum MALDI operates at normal atmospheric environment. In vacuum MALDI, ions are typically produced at 10 mTorr or less while in AP-MALDI ions are formed in atmospheric pressure. 
     AP-MALDI is used in mass spectrometry (MS) in a variety of applications including proteomics and drug discovery fields. AP-MALDI mass spectrometry is often used in proteomics, DNA/RNA/PNA, lipids, oligosaccharides, phosphopeptides, bacteria, small molecules and synthetic polymers, similar applications as available also for vacuum MALDI instruments. 
     The AP-MALDI ion source is easily coupled to an ion trap mass spectrometer or any other MS system equipped with ESI (electrospray ionization) or nanoESI source. 
     The type of a mass spectrometer most widely used with MALDI is the TOF (time-of-flight mass spectrometer) because of its large mass range. The TOF measurement procedure is suited to the MALDI ionization process since the pulsed laser takes individual ‘shots’ rather than working in continuous operation. MALDI-TOF instruments are typically equipped with an “ion mirror,” deflecting ions with an electric field thereby doubling the ion flight path and increasing the resolution. Today, commercial reflectron TOF instruments reach a resolving power m/Δm of well above 20&#39;000 FWHM (full-width half-maximum, Δm defined as the peak width at 50% of peak height). 
     In proteomics, MALDI is used for identifying proteins isolated through gel electrophoresis: SDS-PAGE, size exclusion chromatography, and two-dimensional gel electrophoresis. One method used is peptide mass fingerprinting by MALDI-MS, or with post ionization decay or collision-induced dissociation. 
     IMS. 
     The history of IMS (Imaging Maldi Spectroscopy) began with single cell studies of  Aplysia californica  neurons by matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI/TOF-MS). This was probably the first direct tissue MALDI identification of peptides and tissue profiling based on ion density. (Garden et al.,  J Mass Spectrom  1996; 31:1126-30) A refinement of this technique, imaging MALDI was described in human buccal mucosa, and rat pituitary and pancreas glands using two different approaches: direct targeting of the tissue itself and by analysis of blotted targets previously exposed to the tissue. (Caprioli et al.,  Anal Chem  1997; 69:4751-60) An “MS Image Tool,” using the peptide neurotensin (peak at m/z 1674) significantly improved the data acquisition, speed of IMS, and utilization of the technique. (Stoeckli et al.,  J Am Soc Mass Spectrom  1999; 10:67-71) In a laser, capture microdissection (LCM) study of both tumor and normal human breast tissue fixed in ethyl alcohol, and stained with hematoxylin and eosin, normal tissue, carcinoma in situ, invasive carcinoma, and metastatic carcinoma could be distinguished by their different MALDI spectra. The introduction of “BioMap” software, by applying a baseline correction to the spectra and integrating over the peak of interest, demonstrated mouse brain images of amyloid β, and Aβ peptides. That report was proof of the principle that MALDI images of tissue could be obtained based upon the mass spectrometry mass/charge, m/z peak of interest. (Stoeckli et al.,  Nat Med  2001; 7:493-6) Subsequent profiling and IMS of normal mouse epididymis identified different protein activity (ion densities) throughout the sections. (Chaurand et al.,  Electrophoresis  2002; 23:3125-35) 
     The negative effects on IMS resolution from destructive tissue freezing artifacts, excessive dehydration due to ethanol fixation, paraformaldeyde cationization, embedding artifacts from OCT polymer and agar, and coarse matrix crystal size were first described in a report on spatial profiling of invertebrate ganglia. (Kruse et al.,  J Am Soc Mass Spectrom  2003; 14:752-9) In a follow-up report, they suggested a solution to the problem of freezing artifacts using glycerol and the related compounds ethane-1,2-diol and propane-1,2-diol to stabilize cellular membranes. (Rubakhin et al.,  Anal Chem  2003; 75:5374-80) A subsequent report suggested direct liquid nitrogen immersion of tissue in aluminum wrapping as a means of rapid fixation, but ignored the known consequences of freezer artifact. (Schwartz et al.,  J Mass Spectrom  2003; 38:699-708) Adjunctive histologic staining with methylene blue stained tissues on standard metal plates or indium-tin coated glass slides were shown to be compatible with IMS, but cresyl violet stain decreased IMS signal intensity. (Chaurand et al.,  Anal Chem  2004; 76:1145-55) 
     Tissue blotting with trypsin digestion for MSMS (mass spectrometry mass spectrometry using two mass spectrometers in tandem) data base analysis was shown to be useful in analyte localization, but was destructive to tissue morphology. (Bunch et al.,  Rapid Communications in Mass Spectrometry  2004; 18:3051-60) Others described a less destructive trypsin digest step to their prior tissue blotting technique for IMS. (Rohner et al.,  Mech Ageing Dev  2005; 126:177-85) Another method, matrix-enhanced secondary ion mass spectrometry (ME) SIMS was described and used for direct molecular imaging of the ganglia of the freshwater snail,  Lymnaea stagnalis . (Altelaar et al.,  Anal Chem  2005; 77:735-41) However, this technique presented significant limitations and was proved unsuitable for direct tissue imaging. A refinement of IMS, oversampling with complete sample ablation at each sample position on the target plate, provided significant resolution enhancement with a translation stage raster step size of 25 μM. A 40 μM object could now be resolved with a 100 μM laser. (Jurchen et al.,  Journal of the American Society for Mass Spectrometry  2005; 16:1654-9) 
     Tissue treatments with organic solvents such as chloroform, acetone, hexane, toluene, or xylene were shown to be an effective and rapid method for signal enhancement in MALDI direct tissue profiling. (Lemaire et al.,  Analytical Chemistry  2006; 78:7145-53) These studies demonstrated that solvent treatments partially removed lipids from the tissue surface. Compared to previous studies with ethanol, chloroform/xylene solvent, rinsing is more specific for lipid removal and does not generate delocalization or extraction of most soluble peptides/proteins as tested by immuno-histochemistry experiments. Among all the tested solvents, chloroform and xylene produced the greatest increase in MALDI signal intensity and number of detected peptides/proteins. However, this treatment does not reduce salt adducts as does alcohol treatment. The results suggest that it is possible to detect, after organic rinsing treatments, compounds, such as peptides/proteins present in the cytoplasm, that were masked by lipids in the tissue. 
     Clench et al. reported the development and application of a method using 9-aminoacridine as a matrix for negatively charged ions in MALDI imaging. (Burrell et al.,  J Exp Bot  2007; 58:757-63) Crossman demonstrated the need for thin sections to avoid differential extraction efficiency of matrix solvent in different tissues. (Crossman et al.,  Rapid Communications in Mass Spectrometry  2006; 20:284-90) Pevsner demonstrated direct cellular MALDI identification of proteins in fixed cells and tissues without freezer artifact, tissue corrosion by matrix solvents or the use of tissue blotting. This was confirmed in a later report. (Pevsner et al.,  Direct identification of proteins from cells and tissues using MALDI TOF. Anal Chem .; Pevsner et al.,  J Soc Gynecol Investig  2006; 13:A1-B10; Groseclose et al.,  J Mass Spectrom  2007; 42:254-62) 
     Metal-assisted (MetA) secondary ion mass spectrometry (SIMS), a variation on SIMS as well as matrix-assisted laser desorption/ionization (MALDI) IMS can provide images from tissue, but the duration of these protocols were highly dependent on sample size and technique parameters. The duration of these studies averaged approximately 5 h. (Altelaar et al.,  Nat Protoc  2007; 2:1185-96) 
     Agar et al. studied multiple solvent/matrix combinations. However, the tissue sections at the electron microscopic level demonstrated both freezing artifact and structural distortion, indicating that their method disrupts normal subcellular structures such as mitochondria. (Agar et al.,  Matrix Solution Fixation: Histology -Compatible Tissue Preparation for MALDI Mass Spectrometry Imaging. Anal Chem 2007;.) Baluya et al reported a variation on inkjet-printed matrix application to tissue specimens previously described by Sloane et al. (Baluya et al.,  Anal Chem  2007; 79:6862-7; Sloane et al.,  Mol Cell Proteomics  2002; 1:490-9) The matrix application was of better quality and more reproducible than from specimens prepared by the electrospray and airbrush methods, but still was not completely uniform. A uniform method of tissue matrix application is sublimation. Sublimation is solvent free, rapid, and was successfully used to identify lipids in brain tissue, and more recently described for proteins or peptides. The challenge of IMS in formalin fixed paraffin embedded tissue was first addressed by and then by Pevsner and later by Stauber. (Hankin et al.,  J Am Soc Mass Spectrom  2007; 18:1646-52; Pevsner et al. “Microtubule Associated Proteins (MAP) and Motor Molecules: Direct Tissue MALDI Identification and Imaging.” 2007; Pevsner et al., “Colon Cancer: Protein Biomarkers in Tissue and Body.” 2007; Pevsner et al., “Colorectal Carcinoma—Field Defects in Satellite Tissue.” 2007; Puolitaival et al.,  J Am Soc Mass Spectrom  2008; 19:882-6; Lemaire et al.,  J Proteome Res  2007; 6:1295-305; 48. Stauber et al.,  J Proteome Res  2008; 7:969-78) 
     This invention is illustrated in the experimental section that follows. These examples are set forth to aid in understanding the invention but are not intended to, and should not be construed to, limit in any way the invention as set forth in the claims. 
     EXAMPLE 1 
     New protein biomarkers were identified in murine buccal mucosa after ionizing radiation. The new protein biomarkers were identified from scraping of buccal mucosa obtained fifteen and thirty minutes post ionizing radiation exposure. The biomarkers are as follows:
         DnaJ homolog subfamily C member 1 (DnaJ protein homolog MTJ1) gi|2494160|sp|Q61712.1|DNJC1_MOUSE[2494160. DnaJ domain. DnaJ domains (J-domains) are associated with hsp70 heat-shock system and it is thought that this domain mediates the interaction which is triggered by ionizing radiation.   mCG1463,isoform CRA_b [ Mus musculus]     gi|148683958|gb|EDL15905.1|[14868395]. This protein is involved in transcriptional regulation which is triggered by ionizing radiation.
 
The following three proteins all represent biomarkers of acute radiation exposure which triggers either transcription or post-translational modification.
   Novel protein (B230312A22R1k) [ Mus musculus]  955.87 Da   Amino Acid Sequence: MSGPRKAPED   gi:45593111 Demonstrated 15 minutes post ionizing radiation.   Unnamed protein product [ Mus musculus]  1184.12 Da   Amino Acid Sequence: AKDYYGTGYF   gi|1333908| Demonstrated 15 minutes post ionizing radiation.   Transaldolase [ Mus musculus]  939.07 Da   Amino Acid Sequence: MRHVQAELS   gi|168984609| Demonstrated 30 minutes post ionizing radiation.       

     The identification of specific protein biomarker profiles and new protein biomarkers of ionizing radiation exposure enable reliable associations with dose-exposure, for biodosimetry purposes. This strategy will result in the development of diagnostic tests using a buccal mucosa swab to provide acute clinical quantitative evidence of radiation exposure of 3 Gy or more and allow for accurate and immediate triage of victims of an ionizing radiation mass casualty. 
     EXAMPLE 2 
     Fourteen swiss mice were anesthetized with intraperitoneal ketamine-xylazine (k/x, 80/10 mg/kg ip). Ten mice received 3 Gy to the head. Buccal mucosa scrapings were obtained fifteen minutes (5 mice) and thirty minutes (five mice) post exposure. Buccal mucosa scrapings from the four non-radiated mice were used as controls. All reagents were obtained from Sigma (St. Louis, Mo.) and used unmodified. All samples were placed in 1 μL of 100 mMolar ammonium bicarbonate buffer. Proteins were extracted from all the tissues in this buffer with high pressure (Barocycler, Pressure BioSciences, South Easton, Mass.). The samples were concentrated by lypholization to 200 μL and divided into three components. One for trypsin digestion and liquid chromatography mass spectrometry, LCMS, analysis (bottoms-up proteomics), one for HPLC protein separation and FTMS analysis (top-down proteomics) to identify post-translational modifications (this aliquot was frozen for later analysis), and one for direct identification with matrix assisted laser desorption mass spectrometry, MALDI analysis. The study was conducted under an approved protocol of the New York University School of Medicine Institutional Animal Care and Use Committee, Laboratory Animal Protocol # 080601-01. 
     Sinapic acid (5 mg/mL) 0.3 KL was pipetted onto a conductive MALDI plate, allowed to dry, and covered with an equal amount of sample. All fourteen samples were examined with MALDI in linear mode. The findings were consistent across all four controls, 15 minute (N=5), and 30 minute (N=5) samples,  FIG. 3 . Cyano 4 hydroxy cinnamic acid (5 mg/mL) 0.3 μL was pipetted onto a conductive MALDI plate, allowed to dry, and covered with an equal amount of sample. All fourteen samples were examined with MALDI in reflectron mode. The findings were consistent across all four controls, 15 minute (N=5), and 30 minute (N=5) samples,  FIG. 4 . 
     Results. The linear mode MALDI MS experiment is shown in  FIG. 3 . The lowest spectrum is a control sample. The middle spectrum is a 15 minute post 3 Gy sample. The upper spectrum is a 30 minute post 3 Gy sample. At 15 minutes post 3 Gy, a protein 63870.32 Da, D naJ homolog subfamily C member 1 (DnaJ protein homolog MTJ1) gi|2494160|sp|Q61712.1|DNJC 1_MOUSE[2494160] was demonstrated. DnaJ domains (J-domains) are associated with hsp70 heat-shock system and it is thought that this domain mediates the interaction. At 30 minutes post 3 Gy, a protein 72449.25 Da, mCG1463, isoform CRA_b [ Mus musculus ] gi|148683958|gb|EDL15905.1|[14868395] is tentatively identified in the spectrum. This protein is involved in transcriptional regulation. At 15 and 30 minutes post 3 Gy, the spectrum of peptides is demonstrated in  FIG. 4 , and the peptide amino acid sequences derived with bioinformatics and the corresponding proteins they represent are shown in Table 1. 
                                       TABLE 1               Peptide amino acid sequences and corresponding proteins identified with       bioinformatics at 15 and 30 minutes post 3 Gy.       Peptides at 15 and 30 min post 3 Gy                                15 min   955.87   gi|45593111|novel protein   msgprkaped               (B230312A22Rik) [ Mus musculus ]               1184.12   gi|1333908|unnamed protein product   akdyygtgyf               [ Mus musculus ]           30 min   939.07   gi|168984609|transaldolase   mrhvqaels               [ Mus musculus ].                    
These experiments demonstrated novel changes 15 and 30 minutes post ionizing radiation to the head and neck.
 
     EXAMPLE 3 
     Additional experiments using total body ionizing radiation (N=21), TBI, exposure at 0 (N=3), 1 (N=6), 2 (N=6), and 3 (N=6) Gy were performed (mice). The murine tongue was chosen for examination. The mice were sacrificed at one hour post TBI. MALDI imaging of tongue tissue was combined with high resolution, 1.43 nm, (IR filter visible light 400 nm, Zeiss 63X oil immersion 1.4 NA objective, and 1.4 NA Olympus condenser) bright field microscopy (the smallest mitochondria are 1000 nm); and liquid chromatography mass spectrometry, LCMS, to obtain protein sequence data for exact identification and localization of post TBI protein biomarkers in the tissue sections. Protein extraction from the 3 rd  contiguous section was performed with a Barocycler and digested with trypsin for LCMS examination. Contiguous 5μ cryo-sections were obtained for MALDI imaging, examples of normal, post 1, 2, and 3 Gy,  FIGS. 5-10 ; histology (hematoxylin and eosin, H&amp;E), examples of normal, post 1, 2, and 3 Gy,  FIGS. 5-10 ; and nanoflow liquid chromatography mass spectrometry, LCMS, examples of normal, post 1, 2, and 3 Gy. The molecular weights of the identified proteins were used in the MALDI image prepared with sublimation for tissue localization. (Naftolin et al., Reproductive Sciences 2007; 14:257 A; Pevsner et al., Biomarkers Med 2009; 3:55.  FIG. 5  represents a MALDI image of murine tongue one hour post 1 Gy TBI, longitudinal section. Note peripheral foci of Albumin (Red in image).  FIG. 6  demonstrates the histopathology of murine tongue one hour post 1 Gy TBI, longitudinal section. Note minimal destructive changes in the epithelial cornified spicule layer and edema in the basal region.  FIG. 7  represents a MALDI image Note marked increase in peripheral foci of Albumin compared to the post 1 Gy TBI image (Red in image). 
       FIG. 8  demonstrates the histopathology of murine tongue one hour post 2 Gy TBI, longitudinal section. Note progressive destructive changes in the epithelial layer with disruption of the cornified spicule layer, increased scattered chromatin debris and edema of the sub-basement membrane layer.  FIG. 9  represents a MALDI image of murine tongue one hour post 3 Gy TBI, longitudinal section. Note loss of peripheral foci of albumin consistant with destructive changes in the epithelial layer and increase in central foci of Albumin compared to the post 2 Gy image.  FIG. 10  demonstrates the histopathology of murine tongue one hour post 3 Gy TBI, longitudinal section. Note progressive destructive changes and virtual complete loss of the conified spicule layer corresponding to the loss of peripheral albumin foci (peripheral zone) in the MALDI image, increased scattered chromatin debris, and increased edema of the sub-basement membrane layer. 
     
       
         
               
             
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                 Protein hits 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 AAH11074 
                 BC011074 NID: -  Mus musculus   
               
               
                 K1C17_MOUSE 
                 Keratin, type I cytoskeletal 17 (Cytokeratin-17) (CK-17) (Keratin-17) (K17). - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 Q2M1G8_MOUSE 
                 2410039E07Rik protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 KRMSE1 
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 K1C15_MOUSE 
                 Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) (K15). - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 Q32PO4_MOUSE 
                 Krt2-5 protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 Q8BGZ7_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732475I03 
               
               
                   
                 Product: CYTOKERATIN homolog (10 days neonate skin cDNA, RIKEN full-length 
               
               
                   
                 enriched library, clone: 4732468K03 product: CYTOKERATIN TYPE II homolog) (6 days 
               
               
                   
                 neonate skin cD 
               
               
                 K2C6A_MOUSE 
                 Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK 6A) (K6a keratin) (Keratin- 
               
               
                   
                 6 alpha) (mK6-alpha). -  Mus musculus  (Mouse). 
               
               
                 A55682 
                 keratin 13, type I cytoskeletal - mouse 
               
               
                 Q8K2E4_MOUSE 
                 CDNA sequence BC031593. -  Mus musculus  (Mouse). 
               
               
                 JQ0028 
                 cytokeratin 19 - mouse 
               
               
                 Q9D2K8_MOUSE 
                 0 day neonate head cDNA, RIKEN full-length enriched library, clone: 4833436C19 
               
               
                   
                 product: keratin complex 2, basic, gene 1, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 KRMS2 
                 keratin, type II cytoskelerel - mouse (fragment) 
               
               
                 Q3TTY5_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732404G19 
               
               
                   
                 product: keratin complex 2, basic, gene 17, full insert sequence (10 days neonate 
               
               
                   
                 skin cDNA, RIKEN full-length enriched library, clone: 4732426A12 product: keratin 
               
               
                   
                 complex 
               
               
                 S07330 
                 keratin, epidermal - mouse 
               
               
                 A26135 
                 keratin, 52K type I epidermal (clone pke 52) - mouse (fragment) 
               
               
                 Q80VP7_MOUSE 
                 Hypothetical protein MGC54654. -  Mus musculus  (Mouse). 
               
               
                 Q6IFT3_MOUSE 
                 Keratin Kb40. -  Mus musculus  (Mouse). 
               
               
                 Q8BIS2_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732456N10 
               
               
                   
                 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D (CYTOKERATIN 6D) (CK 6D) (K6D 
               
               
                   
                 KERATIN). -  Mus musculus  (Mouse). 
               
               
                 Q6NXH9_MOUSE 
                 Type II keratin Kb36. -  Mus musculus  (Mouse). 
               
               
                 K2C1B_MOUSE 
                 Keratin, type II cytoskeletal 1b (Type II keratin Kb39) (Embryonic type II 
               
               
                   
                 keratin-1). -  Mus musculus  (Mouse). 
               
               
                 BAE40567 
                 AK168726 NID: -  Mus musculus   
               
               
                 K2C4_MOUSE 
                 Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) 
               
               
                   
                 (Cytoskeletal 57 kDa keratin). -  Mus musculus  (Mouse). 
               
               
                 Q3UV17_MOUSE 
                 Adult female vagina cDNA, RIKEN full-length enriched library, clone: 9930024P18 
               
               
                   
                 product: similar to Keratin 2p. -  Mus musculus  (Mouse). 
               
               
                 I59009 
                 epidermal keratin subunit II - mouse 
               
               
                 Q9Z1R9_MOUSE 
                 Trypsinogen 16 (Protease, serine, 1). -  Mus musculus  (Mouse). 
               
               
                 Q9CXH5_MOUSE 
                 17 days embryo head cDNA, RIKEN full-length enriched library, clone: 3300O01P16 
               
               
                   
                 product: hemoglobin, beta adult major chain, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 Q6IME9_MOUSE 
                 Type-II keratin Kb35. -  Mus musculus  (Mouse). 
               
               
                 Q9JJ20_MOUSE 
                 14-3-3 protein sigma. -  Mus musculus  (Mouse). 
               
               
                 H2B1B_MOUSE 
                 Histone H2B type 1-B (h2B-143). -  Mus musculus  (Mouse). 
               
               
                 Q8BKC6_MOUSE 
                 0 day neonate eyeball cDNA, RIKEN full-length enriched library, clone: E130120J09 
               
               
                   
                 product: hypothetical Intermediate filament proteins containing protein, full 
               
               
                   
                 insert sequence. (Fragment). -  Mus musculus  (Mouse). 
               
               
                 I48739 
                 MHa2(keratin acidic 2) - mouse 
               
               
                 H2A2A_HUMAN 
                 Histone H2A type 2-A (H2A.2). -  Homo sapiens  (Human). 
               
               
                 HAMS 
                 hemoglobin alpha chains - mouse 
               
               
                 BAC33789 
                 AK049517 NID: -  Mus musculus   
               
               
                   
               
               
                 Probability Based Mowse Score 
               
               
                 Ions score is −10 * Log(P), where P is the probability that the observed match is a random event. 
               
               
                 Individual ions scores &gt;38 indicate identity or extensive homology (p &lt; 0.05). 
               
               
                 Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits. 
               
             
          
         
       
     
     Table 2 provides the results of a Mascot search of proteins in normal murine tongue. No albumin was identified. 
     
       
         
               
             
               
               
             
               
             
               
               
             
           
               
                 TABLE 3 
               
               
                   
               
               
                 Peptide Summary Report (../data/20090408/FdAifuwe.dat) 
               
               
                 {MATRIX SCIENCE} Mascot Seach Results 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 User 
                 NYUSOM 
               
               
                 Email 
                 seagea01@nyumc.org 
               
               
                 Search title 
                   
               
               
                 MS data file 
                 E:\20090324_LCMS_Samples\9_001_CID.mgf 
               
               
                 Database 
                 MSDB 20060831 (3239079 sequences; 1079594700 residues) 
               
               
                 Taxonomy 
                 Mus musculus (house mouse) (90914 sequences) 
               
               
                 Timestamp 
                 8 Apr 2009 at 13:57:09 GMT 
               
             
          
           
               
                 Protein hits 
               
             
          
           
               
                 Q8BGZ7_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732475IO3 
               
               
                   
                 prdouct: CYTOKERATIN homolog (10 days neonate skin cDNA, RIKEN full-length 
               
               
                   
                 enriched library, clone: 4732468KO3 product: CYTOKERATIN TYPE II homolog) (6 days 
               
               
                   
                 neonate skin cD 
               
               
                 K2C1_MOUSE 
                 Keratin, type II cytoskeletal 1 (Cytokeratin-1) (CK-1) (Keratin-1) (K1) (67 kDa 
               
               
                   
                 cytokeratin). -  Mus musculus  (Mouse). 
               
               
                 Q9D2K8_MOUSE 
                 0 day neonate head cDNA, RIKEN full-length enriched library, clone: 4833436C19 
               
               
                   
                 prdouct: keratin complex 2, basic, gene 1, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 Q32P04_MOUSE 
                 Krt2-5 protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 K2C6A_MOUSE 
                 Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK 6A) (K6a keratin) (Keratin- 
               
               
                   
                 6 alpha) (mK6-alpha). -  Mus musculus  (Mouse). 
               
               
                 Q80VP7_MOUSE 
                 Hypothetical protein MGC54654. -  Mus musculus  ; (Mouse). 
               
               
                 Q8BIS2_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732456N10 
               
               
                   
                 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D (CYTOKERATIN 6D) (CK 6D) (K6D 
               
               
                   
                 KERATIN). -  Mus musculus  (Mouse). 
               
               
                 Q9CXH5_MOUSE 
                 17 days embryo head cDNA, RIKEN full-length enriched library, clone: 3300001P16 
               
               
                   
                 product: hemoglobin, beta adult major chain, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 KRMSE1 
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 Q6NXH9_MOUSE 
                 Type II keratin Kb36. -  Mus musculus  (Mouse). 
               
               
                 K2C1B_MOUSE 
                 Keratin, type II cytoskeletal 1b (Type II keratin Kb39) (Embryonic type II 
               
               
                   
                 keratin-1). -  Mus musculus  (Mouse). 
               
               
                 Q6IFZ9_MOUSE 
                 Type II keratin Kb37. -  Mus musculus  (Mouse). 
               
               
                 Q3TTY5_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732404G19 
               
               
                   
                 product: keratin complex 2, basic, gene 17, full insert sequence (10 days neonate 
               
               
                   
                 skin cDNA, RIKEN full-length enriched library, clone: 4732426A12 product: keratin 
               
               
                   
                 complex 
               
               
                 Q6IFT3_MOUSE 
                 Keratin Kb40. -  Mus musculus  (Mouse). 
               
               
                 Q3UV17_MOUSE 
                 Adult female vagina cDNA, RIKEN full-length enriched library, clone: 9930024P18 
               
               
                   
                 product: similar to Keratin 2p. -  Mus musculus  (Mouse). 
               
               
                 I59009 
                 epidermal keratin subunit II - mouse 
               
               
                 HAMS 
                 hemoglobin alpha chains - mouse 
               
               
                 K2C4_MOUSE 
                 Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) 
               
               
                   
                 (Cytoskeletal 57 kDa keratin). -  Mus musculus  (Mouse). 
               
               
                 Q6IME9_MOUSE 
                 Type-II keratin Kb35. -  Mus musculus  (Mouse). 
               
               
                 K2C8_MOUSE 
                 Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) 
               
               
                   
                 (Cytokeratin endo A). -  Mus musculus . (Mouse). 
               
               
                 Q3TV03_MOUSE 
                 Adult male stomach cDNA, RIKEN full-length enriched library, clone: 2210414C06 
               
               
                   
                 product: albumin 1, full insert sequence. -  Mus musculus  (Mouse). 
               
               
                 Q9Z1R9_MOUSE 
                 Trypsinogen 16 (Protease, serine, 1). -  Mus musculus  (Mouse). 
               
               
                 JQ0028 
                 cytokeratin 19 - mouse 
               
               
                 Q2M1G8_MOUSE 
                 2410039E07Rik protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 AAH11074 
                 BC011074 NID: -  Mus musculus   
               
               
                   
               
               
                 Probability Based Mowse Score 
               
               
                 Ions score is −10 * Log(P), where P is the probability that the observed match is a random event. 
               
               
                 Individual ions scores &gt;38 indicate identity or extensive homology (p &lt; 0.05). 
               
               
                 Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits. 
               
             
          
         
       
     
     Table 3 lists LCMS identified proteins with Mascot search of proteins in murine tongue one hour post 1 Gy TBI. Albumin appears at the end of the list and corresponds to the findings in the MALDI image one hour post 1 Gy. 
     
       
         
               
             
               
               
             
               
             
               
               
             
           
               
                 TABLE 4 
               
               
                   
               
               
                 Peptide Summary Report (../data/20090408/FdAifsSE.dat) 
               
               
                 {MATRIX SCIENCE} Mascot Seach Results 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 User 
                 NYUSOM 
               
               
                 Email 
                 seagea01@nyumc.org 
               
               
                 Search title 
                   
               
               
                 MS data file 
                 E:\20090324_LCMS_Samples\10_001_CID.mgf 
               
               
                 Database 
                 MSDB 20060831 (3239079 sequences; 1079594700 residues) 
               
               
                 Taxonomy 
                 Mus musculus (house mouse) (90914 sequences) 
               
               
                 Timestamp 
                 8 Apr 2009 at 14:03:15 GMT 
               
             
          
           
               
                 Protein hits 
               
             
          
           
               
                 KRMSE1 
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 Q9CY54_MOUSE 
                 13 days embryo liver cDNA, RIKEN full-length enriched library, clone: 2500004H04 
               
               
                   
                 product: hemoglobin, beta adult major chain, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 HAMS 
                 hemoglobin alpha chains - mouse 
               
               
                 Q9CY06_MOUSE 
                 13 days embryo liver cDNA, RIKEN full-length enriched library, clone: 2510040P05 
               
               
                   
                 product: hemoglobin, beta adult major chain, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 AAH11074 
                 BC011074 NID: - Mus musculus 
               
               
                 HBMS 
                 hemoglobin beta major chain - mouse 
               
               
                 Q3TTY5_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732404G19 
               
               
                   
                 product: keratin complex 2, basic, gene 17, full insert sequence (10 days neonate 
               
               
                   
                 skin, cDNA, RIKEN full-length enriched library, clone: 4732426A12 product: keratin 
               
               
                   
                 complex 
               
               
                 Q3UJH8_MOUSE 
                 16 days embryo heart cDNA, RIKEN full-length enriched library, clone: I920001F12 
               
               
                   
                 product: glutamate oxaloacetate transaminase 1, soluble, full insert sequence. - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 Q8BGZ7_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732475I03 
               
               
                   
                 product: CYTOKERATIN homolog (10 days neonate skin cDNA, RIKEN full-length 
               
               
                   
                 enriched library, clone: 4732468K03 product: CYTOKERATIN TYPE II homolog) (6 days 
               
               
                   
                 neonate skin cD 
               
               
                 K1C17_MOUSE 
                 Keratin, type I cytoskeletal 17 (Cytokeratin-17) (CK-17) (Keratin-17) (K17). - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 Q9R0S6_MOUSE 
                 Beta-1-globin (Fragment). -  Mus musculus  (Mouse). 
               
               
                 K2C1_MOUSE 
                 Keratin, type II cytoskeletal 1 (Cytokeratin-1) (CK-1) (Keratin-1) (K1) (67 kDa 
               
               
                   
                 cytokeratin). -  Mus musculus  (Mouse). 
               
               
                 Q9D2K8_MOUSE 
                 0 day neonate head cDNA, RIKEN full-length enriched library, clone: 4833436C19 
               
               
                   
                 product: keratin complex 2, basic, gene 1, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 A55682 
                 keratin 13, type I cytoskeletal - mouse 
               
               
                 Q32P04_MOUSE 
                 Krt2-5 protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 CAA33084 
                 MMHFABP NID: -  Mus musculus   
               
               
                 Q80VP7_MOUSE 
                 Hypothetical protein MGC54654. -  Mus musculus  (Mouse). 
               
               
                 Q8BIS2_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732456N1O 
               
               
                   
                 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D (CYTOKERATIN 6D) (CK 6D) 
               
               
                   
                 (K6D KERATIN). -  Mus musculus  (Mouse). 
               
               
                 Q6NXH9_MOUSE 
                 Type II keratin Kb36. -  Mus musculus  (Mouse). 
               
               
                 K1C15_MOUSE 
                 Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) (K15). - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 FABPH_MOUSE 
                 Fatty acid-binding protein, heart (H-FABP) (Heart-type fatty acid-binding 
               
               
                   
                 protein) (Mammary-derived growth inhibitor) (MDGI). -  Mus musculus  (Mouse). 
               
               
                 Q3UV17_MOUSE 
                 Adult female vagina cDNA, RIKEN full-length enriched library, clone: 9930024P18 
               
               
                   
                 product: similar to Keratin 2p. -  Mus musculus  (Mouse). 
               
               
                 K2C4_MOUSE 
                 Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) 
               
               
                   
                 (Cytoskelatal 57 kDa keratin). -  Mus musculus  (Mouse). 
               
               
                 Q6IME9_MOUSE 
                 Type-II keratin Kb35. -  Mus musculus  (Mouse). 
               
               
                 Q6IFT3_MOUSE 
                 Keratin Kb40. -  Mus musculus  (Mouse). 
               
               
                 Q8VCW2_MOUSE 
                 RIKEN cDNA 4631426H08. -  Mus musculus  (Mouse). 
               
               
                 Q2M1G8_MOUSE 
                 2410039E07Rik protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                   
                 Fatty acid-binding protein, adipocyte (AFABP) (Adipocyte lipid-binding protein) 
               
               
                 FABPA_MOUSE 
                 (ALBP) (A-FABP) (P2 adipocyte protein) (Myelin P2 protein homolog) (3T3-L1 lipid- 
               
               
                   
                 binding protein) (422 protein) (P15). -  Mus musculus  (Mouse). 
               
               
                 I59009 
                 epidermal keratin subunit II - mouse 
               
               
                 Q3TV03_MOUSE 
                 Adult male stomach cDNA, RIKEN full-length enriched library, clone: 2210414C06 
               
               
                   
                 product: albumin 1, full insert sequence. -  Mus musculus  (Mouse). 
               
               
                 DEMSMC 
                 malate dehydrogenase (EC 1.1.1.37), cytosolic - mouse 
               
               
                 JQ0028 
                 cytokeratin 19 - mouse 
               
               
                 Q3UDM1_MOUSE 
                 Bone marrow macrophage cDNA, RIKEN full-length enriched library, clone: 
               
               
                   
                 G530119K21 product: ATP-binding cassette, sub-family C (CFTR/MRP), member 1, full 
               
               
                   
                 insert sequence. (Fragment). -  Mus musculus  (Mouse). 
               
               
                 Q3UBW7_MOUSE 
                 Bone marrow macrophage cDNA, RIKEN full-length enriched library, clone: 
               
               
                   
                 I830015F18 product: tranrferrin, full insert sequence. -  Mus musculus  (Mouse). 
               
               
                   
               
               
                 Probability Based Mowse Score 
               
               
                 Ions score is −10 * Log(P), where P is the probability that the observed match is a random event. 
               
               
                 Individual ions scores &gt;38 indicate identity or extensive homology (p &lt; 0.05). 
               
               
                 Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits. 
               
             
          
         
       
     
     Table 4 lists LCMS identified proteins with Mascot search of proteins in murine tongue one hour post 2 Gy TBI. Albumin appears next to last in the list. 
     
       
         
               
             
               
               
             
               
             
               
               
             
           
               
                 TABLE 5 
               
               
                   
               
               
                 Peptide Summary Report (../data/20090408/FdAifgEL.dat) 
               
               
                 {MATRIX SCIENCE} Mascot Seach Results 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 User 
                 NYUSOM 
               
               
                 Email 
                 seagea01@nyumc.org 
               
               
                 Search title 
                   
               
               
                 MS data file 
                 E:\20090324_LCMS_Samples\16_001_CID.mgf 
               
               
                 Database 
                 MSDB 20060831 (3239079 sequences; 1079594700 residues) 
               
               
                 Taxonomy 
                 Mus musculus (house mouse) (90914 sequences) 
               
               
                 Timestamp 
                 8 Apr 2009 at 14:05:43 GMT 
               
             
          
           
               
                 Protein hits 
               
             
          
           
               
                 HAMS 
                 hemoglobin alpha chains - mouse 
               
               
                 Q9CY12_MOUSE 
                 13 days embryo liver cDNA, RIKEN full-length enriched library, 
               
               
                   
                 clone: 2510039D09 product: hemoglobin, beta adult major chain, full insert 
               
               
                   
                 sequence. -  Mus musculus  (Mouse). 
               
               
                 Q8BGZ7_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                   
                 clone: 4732475I03 product: CYTOKERATIN homolog (10 days neonate skin cDNA, RIKEN 
               
               
                   
                 full-length enriched library, clone: 4732468K03 product: CYTOKERATIN TYPE II 
               
               
                   
                 homolog) (6 days neonate skin cD 
               
               
                 Q32P04_MOUSE 
                 Krt2-5 protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 Q3TV03_MOUSE 
                 Adult male stomach cDNA, RIKEN full-length enriched library, clone: 2210414C06 
               
               
                   
                 product: albumin 1, full insert sequence. -  Mus musculus  (Mouse). 
               
               
                 KRMSE1 
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 K1C15_MOUSE 
                 Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) (K15). - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 Q8BIS2_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                   
                 clone: 4732456N10 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D 
               
               
                   
                 (CYTOKERATIN 6D) (CK 6D) (K6D KERATIN). -  Mus musculus  (Mouse). 
               
               
                 Q8BGZ7_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732475I03 
               
               
                   
                 product: CYTOKERATIN homolog (10 days neonate skin cDNA, RIKEN full-length 
               
               
                   
                 enriched library, clone: 4732468K03 product: CYTOKERATIN TYPE II homolog) (6 days 
               
               
                   
                 neonate skin cD 
               
               
                 K2C6A_MOUSE 
                 Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK 6A) (K6a keratin) (Keratin- 
               
               
                   
                 6 alpha) (mK6-alpha). -  Mus musculus  (Mouse). 
               
               
                 Q32P04_MOUSE 
                 Krt2-5 protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 Q8K2E4_MOUSE 
                 CDNA sequence BC031593. -  Mus musculus  (Mouse). 
               
               
                 K2C1_MOUSE 
                 Keretin, type II cytoskeletal 1 (Cytokeratin-1) (CK-1) (Keratin-1) (K1) (67 kDa 
               
               
                   
                 cytokeratin). -  Mus musculus  (Mouse). 
               
               
                 Q9D2K8_MOUSE 
                 0 day neonate head cDNA, RIKEN full-length enriched library, clone: 4833436C19 
               
               
                   
                 product: keratin complex 2, basic, gene 1, full insert sequence. -  Mus musculus   
               
               
                   
                 (Mouse). 
               
               
                 Q8BIS2_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732456N10 
               
               
                   
                 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D (CYTOKERATIN 6D) (CK 6D) (K6D 
               
               
                   
                 KERATIN). -  Mus musculus  (Mouse). 
               
               
                 Q80VP7_MOUSE 
                 Hypothetical protein MGC54654. -  Mus musculus  (Mouse). 
               
               
                 Q6NXH9_MOUSE 
                 Type II keratin Kb36. -  Mus musculus  (Mouse). 
               
               
                 Q6IFT3_MOUSE 
                 Keratin Kb40. -  Mus musculus  (Mouse). 
               
               
                 K2C1B_MOUSE 
                 Keratin, type II cytoskeletal 1b (Type II keratin Kb39) (Embryonic type II 
               
               
                   
                 keratin-1). -  Mus musculus  (Mouse). 
               
               
                 Q3UV17_MOUSE 
                 Adult female vagina cDNA, RIKEN full-length enriched library, clone: 9930024P18 
               
               
                   
                 product: similar to Keratin 2p. -  Mus musculus  (Mouse). 
               
               
                 K2C4_MOUSE 
                 Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) 
               
               
                   
                 (Cytoskeletal 57 kDa keratin). -  Mus musculus  (Mouse). 
               
               
                 AAH11074 
                 BC011074 NID: -  Mus musculus   
               
               
                 BAA85657 
                 AB033744 NID: -  Mus musculus   
               
               
                 Q6IFZ9_MOUSE 
                 Type II keratin Kb37. -  Mus musculus  (Mouse). 
               
               
                 I59009 
                 epidermal keratin subunit II - mouse 
               
               
                 K1C17_MOUSE 
                 Keratin, type I cytoskeletal 17 (Cytokeratin-17) (CK-17) (Keratin-17) (K17). - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 Q6IFX2_MOUSE 
                 Type I keratin KA22. -  Mus musculus  (Mouse). 
               
               
                 Q6IME9_MOUSE 
                 Type-II keratin Kb35. -  Mus musculus  (Mouse). 
               
               
                 BAE40567 
                 AK168726 NID: -  Mus musculus   
               
               
                 AAD01692 
                 AF021836 NID: -  Mus musculus   
               
               
                 Q3USS4_MOUSE 
                 Adult male corpora quadrigemina cDNA, RIKEN full-length enriched library, clone: 
               
               
                   
                 B230315D14 product: glial fibrillary acidic protein, full insert sequence. -  Mus   
               
               
                   
                   musculus  (Mouse). 
               
               
                 Q3TTY5_MOUSE 
                 10 days neonate skin cDNA, RIKEN full-length enriched library, clone: 4732404G19 
               
               
                   
                 product: keratin complex 2, basic, gene 17, full insert sequence (10 days neonate 
               
               
                   
                 skin cDNA, RIKEN full-length enriched library, clone: 4732426A12 product: keratin 
               
               
                   
                 complex 
               
               
                 K1C15_MOUSE 
                 Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) (K15). - 
               
               
                   
                   Mus musculus  (Mouse). 
               
               
                 Q3TWV0_MOUSE 
                 Osteoclast-like cell cDNA, RIKEN full-length enriched library, clone: I420023H06 
               
               
                   
                 product: vimentin, full insert sequence. -  Mus musculus  (Mouse). 
               
               
                 Q9JKB4_MOUSE 
                 Epidermal keratin 10 (Fragment). -  Mus musculus  (Mouse). 
               
               
                 Q8VCW2_MOUSE 
                 RIKEN cDNA 4631426H08. -  Mus musculus  (Mouse). 
               
               
                 JQO028 
                 cytokeratin 19 - mouse 
               
               
                 KRMSE1 
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 JC4030 
                 DnaJ-like protein MTJ1 - mouse 
               
               
                 E2AK4_MOUSE 
                 Eukaryotic translation initiation factor 2-alpha kinase 4 (EC 2.7.11.1) (GCN2- 
               
               
                   
                 like protein) (mGCN2). -  Mus muaculus  (Mouse). 
               
               
                 BAB27580 
                 AK011380 NID: -  Mus musculus   
               
               
                   
               
               
                 Probability Based Mowse Score 
               
               
                 Ions score is −10 * Log(P), where P is the probability that the observed match is a random event. 
               
               
                 Individual ions scores &gt;38 indicate identity or extensive homology (p &lt; 0.05). 
               
               
                 Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits. 
               
             
          
         
       
     
     Table 5 lists LCMS identified proteins from two separate samples 16 and 19. Mascot search of proteins in murine tongue one hour post 3 Gy TBI. The absence of albumin in these two 3 Gy samples, it was present in other 3 Gy samples (data not shown) is consistent with the marked tissue destruction, and very small amount noted in the MALDI image. 
     EXAMPLE 4 
     It has been shown (MHB) that latent transforming growth factor-β, LTGF-β, is rapidly activated in vivo after ionizing radiation (IR). This is evidenced by increased immunoreactivity of TGFβ epitopes that are masked by the latent complex. (Barcellos-Hoff  Mol Biol Cell  1994; 5:139a; Barcellos-Hoff et al.,  Am J Pathol  1995; 147:1228-37; Barcellos-Hoff et al.,  Molec Endocrin  1996; 10: 1077-83; Barcellos-Hoff et al.,  Mol Endocrinol  1996; 10:1077-83; Barcellos- Hoff J Mammary Gland Biol Neoplasia  1996; 1:353-63; Ehrhart et al.,  FASEB J  1997; 11:991-1002; Barcellos-Hoff et al.,  Breast Cancer Res  2000; 2:92-9; Barcellos-Hoff et al.,  Nat Rev Cancer  2005; 5:867-75; Jobling et al.,  Radiat Res  2006; 166:839-48; Jobling et al.,  Radiat Res  2006; 166:839-848). Because reactive oxygen species, ROS, are a product of the interaction of IR with water or with cell membranes, it was postulated that the rapid activation of TGFβ in vivo could be due to ROS generated by IR if the protein itself contained redox sensitive amino acids. We were able to demonstrate the monomers of TGFβ and the latency associated protein, LAP, in MALDI images one hour post 1, 2, and 3 Gy,  FIGS. 18 ,  19 ,  20 . Note the approximately 10 Da variance in all three images. This molecular weight variance and spatial resolution can be improved, infra vida. Therefore TGFβ can also be used as a protein biomarker of absorbed dose. Quantization can be obtained from trypsin digests studied by LCMS.  FIGS. 11 ,  12  and  13  depict LAP monomers, light blue in the image. 
     Additional dose specific protein biomarkers identified from other LCMS studies were not seen in normal tissue. Hemoglobin subunit a, and parathymosinare only found one hour post 1 Gy; Fatty Acid Binding Protein Adipocyte seen 1 hour post 1 and 2 Gy IR; and triosphosphate Isomerase, and superoxide dismutase seen at one hour post 2 Gy are not seen in normal tissue or at one hour post 1 Gy. 
       FIG. 14  depicts da hemoglobin subunit α, and parathymosinare only found one hour post 1 Gy IR, red in the image.  FIG. 15  depicts fatty acid-binding protein adipocyte seen 1 hour post 1 and 2 Gy IR, red in the image.  FIG. 16  depicts one hour post 2 Gy exposure. Triosphosphate isomerase and superoxide dismutase are seen one hour post 2 Gy, but not seen in normal tissue or at one hour post 1 Gy (red in the image).  FIG. 17  demonstrates hemaglobin α chains and BC011074 NID seen one hour post 3 Gy exposure. 
     The results with MALDI imaging, high resolution microscopy, and LCMS are proof of principle that post TBI protein biomarkers can be identified and used as measures of absorbed radiation dose at 1, 2, and 3 Gy with our current technology. Identification of a larger library of post TBI proteins will improve specificity of both the POC triage kit and the HT device. This will dramatically limit false positives and false negatives. This will be achieved with a combination of strategies including higher resolution bright field microscopy, atomic force microscopy of irradiated tissue for higher spatial resolution of tissue loci of proteins by co-registration with the MALDESI images. (Collier et al.,  Anal Chem  2008; 80:4994-5001; Bereman et al.,  Rapid Commun Mass Spectrom  2008; 22:1563-6; Sampson et al.,  J Am Soc Mass Spectrom  2009; 20:667-73). Separation of extracted proteins is performed by 2D cation exchange LCMS with high-throughput electron capture dissociation (ECD) analysis to identify post translational modification of IR induced protein biomarkers; modifying sublimation matrix application by varying time, temperature and distance between the matrix powder platform and the mounted tissue,  FIG. 16 ; thinner tissue sections with a high performance cryostat; and complete removal of tissue embedding media polymer residue by varying solvent baths. 
     The application of matrix assisted laser distortion electro-spray ionization Fourier transform ion cyclotron mass spectrometry, FTICR, for both liquid samples and imaging, MALDESI imaging, will improve the isotopic resolution by 2 orders of magnitude. The MALDI system full width half mass, FWHM, isotopic resolution is 2500 Da. The MALDESI system full width half mass, FWHM, isotopic resolution is 250,000 Da. The spatial resolution of MALDI images will improve with laser M 2  beam quality closer to 1, increased repetition rate, Hz, fluence, mj/s (Watts), Energy, E, mj/pulse, pulse width, increased work function, smaller spot size, and, optics (beam focus); and decremental sample stage movement from microns to nanometers. 
     The following Table 6 provides examples of other potential biomarkers of ionizing radiation isolated from buccal tissue in subsequent examples obtained according to the procedures set forth in Example 3. 
     
       
         
               
             
           
               
                 TABLE 6 
               
               
                   
               
             
             
               
                 K1C10_MOUSE 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Mus musculus  GN = Krt10 PE = 1 SV = 3 
               
               
                 ALBU_MOUSE 
               
               
                 Serum albumin OS =  Mus musculus  GN = Alb PE = 1 SV = 3 
               
               
                 K2C75_MOUSE 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Mus musculus  GN = Krt75 PE = 1 SV = 1 
               
               
                 K2C1_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Mus musculus  GN = Krt1 PE = 1 SV = 4 
               
               
                 K1C14_MOUSE 
               
               
                 Keratin, type I cytoskeletal 14 OS =  Mus musculus  GN = Krt14 PE = 1 SV = 2 
               
               
                 K2C5_MOUSE 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Mus musculus  GN = Krt5 PE = 2 SV = 1 
               
               
                 K1C15_MOUSE 
               
               
                 Keratin, type I cytoskeletal 15 OS =  Mus musculus  GN = Krt15 PE = 1 SV = 2 
               
               
                 K1C13_MOUSE 
               
               
                 Keratin, type I cytoskeletal 13 OS =  Mus musculus  GN = Krt13 PE = 2 SV = 2 
               
               
                 K2C6A_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Mus musculus  GN = Krt6a PE = 2 SV = 3 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Mus musculus  GN = Krt4 PE = 2 SV = 2 
               
               
                 K2C73_MOUSE 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Mus musculus  GN = Krt73 PE = 2 SV = 1 
               
               
                 K1C17_MOUSE 
               
               
                 Keratin, type I cytoskeletal 17 OS =  Mus musculus  GN = Krt17 PE = 1 SV = 3 
               
               
                 K22O_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Mus musculus  GN = Krt76 PE = 2 SV = 1 
               
               
                 K2C79_MOUSE 
               
               
                 Keratin, type II cytoskeletal 79 OS =  Mus musculus  GN = Krt79 PE = 2 SV = 2 
               
               
                 HBA_MOUSE 
               
               
                 Hemoglobin subunit alpha OS =  Mus musculus  GN = Hba PE = 1 SV = 2 
               
               
                 AATC_MOUSE 
               
               
                 Aspartate aminotransferase, cytoplasmic OS =  Mus musculus  GN = Got1 PE = 1 SV = 2 
               
               
                 K1C19_MOUSE 
               
               
                 Keratin, type I cytoskeletal 19 OS =  Mus musculus  GN = Krt19 PE = 2 SV = 1 
               
               
                 K1C42_MOUSE 
               
               
                 Keratin, type I cytoskeletal 42 OS =  Mus musculus  GN = Krt42 PE = 1 SV = 1 
               
               
                 K2C1B_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1b OS =  Mus musculus  GN = Krt77 PE = 1 SV = 1 
               
               
                 K2C71_MOUSE 
               
               
                 Keratin, type II cytoskeletal 71 OS =  Mus musculus  GN = Krt71 PE = 1 SV = 1 
               
               
                 K2C74_MOUSE 
               
               
                 Keratin, type II cytoskeletal 74 OS =  Mus musculus  GN = Krt74 PE = 2 SV = 1 
               
               
                 K2C8_MOUSE 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Mus musculus  GN = Krt8 PE = 1 SV = 4 
               
               
                 K1C27_MOUSE 
               
               
                 Keratin, type I cytoskeletal 27 OS =  Mus musculus  GN = Krt27 PE = 1 SV = 1 
               
               
                 K2C72_MOUSE 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Mus musculus  GN = Krt72 PE = 2 SV = 1 
               
               
                 FABP4_MOUSE 
               
               
                 Fatty acid-binding protein, adipocyte OS =  Mus musculus  GN = Fabp4 PE = 1 SV = 3 
               
               
                 PTMS_MOUSE 
               
               
                 Parathymosin OS =  Mus musculus  GN = Ptms PE = 2 SV = 3 1 
               
               
                 K222P_MOUSE 
               
               
                 Keratin-like protein KRT222 OS =  Mus musculus  GN = Krt222 PE = 2 SV = 1 
               
               
                 K22E_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Mus musculus  GN = Krt2 PE = 1 SV = 1 
               
               
                 HBB2_MOUSE 
               
               
                 Hemoglobin subunit beta-2 OS =  Mus musculus  GN = Hbb-b2 PE = 1 SV = 2 
               
               
                 TPIS_MOUSE 
               
               
                 Triosephosphate isomerase OS =  Mus musculus  GN = Tpi1 PE = 1 SV = 3 
               
               
                 FABPH_MOUSE 
               
               
                 Fatty acid-binding protein, heart OS =  Mus musculus  GN = Fabp3 PE = 1 SV = 5 
               
               
                 BICD2_MOUSE 
               
               
                 Protein bicaudal D homolog 2 OS =  Mus musculus  GN = Bicd2 PE = 1 SV = 1 
               
               
                 K2C1_PANTR 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Pan troglodytes  GN = KRT1 PE = 2 SV = 1 
               
               
                 K2C1_HUMAN 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Homo sapiens  GN = KRT1 PE = 1 SV = 5 
               
               
                 K1C9_HUMAN 
               
               
                 Keratin, type I cytoskeletal 9 OS =  Homo sapiens  GN = KRT9 PE = 1 SV = 2 
               
               
                 K22E_HUMAN 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Homo sapiens  GN = KRT2 PE = 1 SV = 1 
               
               
                 K1C10_HUMAN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Homo sapiens  GN = KRT10 PE = 1 SV = 4 
               
               
                 K1C10_CANFA 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Canis familiaris  GN = KRT10 PE = 2 SV = 1 
               
               
                 K2C1_RAT 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Rattus norvegicus  GN = Krt1 PE = 2 SV = 1 
               
               
                 K22E_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Mus musculus  GN = Krt2 PE = 1 SV = 1 
               
               
                 K1C14_HUMAN 
               
               
                 Keratin, type I cytoskeletal 14 OS =  Homo sapiens  GN = KRT14 PE = 1 SV = 3 
               
               
                 K1C10_BOVIN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Bos taurus  GN = KRT10 PE = 3 SV = 1 
               
               
                 K2C75_BOVIN 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Bos taurus  GN = KRT75 PE = 2 SV = 1 
               
               
                 TRYP_PIG 
               
               
                 Trypsin OS = Sus scrofa PE = 1 SV = 1 
               
               
                 K2C75_RAT 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Rattus norvegicus  GN = Krt75 PE = 2 SV = 2 
               
               
                 K2C6A_RAT 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Rattus norvegicus  GN = Krt6a PE = 1 SV = 1 
               
               
                 ALBU_MOUSE 
               
               
                 Serum albumin OS =  Mus musculus  GN = Alb PE = 1 SV = 3 
               
               
                 K2C4_RAT 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Rattus norvegicus  GN = Krt4 PE = 2 SV = 1 
               
               
                 K2C73_HUMAN 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Homo sapiens  GN = KRT73 PE = 1 SV = 1 
               
               
                 K2C6A_HUMAN 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Homo sapiens  GN = KRT6A PE = 1 SV = 3 
               
               
                 K2C73_MOUSE 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Mus musculus  GN = Krt73 PE = 2 SV = 1 
               
               
                 K2C1B_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1b OS =  Mus musculus  GN = Krt77 PE = 1 SV = 1 
               
               
                 K2C5_RAT 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Rattus norvegicus  GN = Krt5 PE = 1 SV = 1 
               
               
                 K2C6A_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Mus musculus  GN = Krt6a PE = 2 SV = 3 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Mus musculus  GN = Krt4 PE = 2 SV = 2 
               
               
                 K22O_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Mus musculus  GN = Krt76 PE = 2 SV = 1 
               
               
                 K2C3_HUMAN 
               
               
                 Keratin, type II cytoskeletal 3 OS =  Homo sapiens  GN = KRT3 PE = 1 SV = 2 
               
               
                 K2C72_HUMAN 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Homo sapiens  GN = KRT72 PE = 1 SV = 2 
               
               
                 HBD_TARSY 
               
               
                 Hemoglobin subunit delta OS =  Tarsius syrichta  GN = HBD PE = 2 SV = 2 3 
               
               
                 K1C13_MOUSE 
               
               
                 Keratin, type I cytoskeletal 13 OS =  Mus musculus  GN = Krt13 PE = 2 SV = 2 
               
               
                 HBA_CRIGA 
               
               
                 Hemoglobin subunit alpha OS =  Cricetomys gambianus  GN = HBA PE = 1 SV = 2 
               
               
                 TRY1_RAT 
               
               
                 Anionic trypsin-1 OS =  Rattus norvegicus  GN = Prss1 PE = 1 SV = 1 
               
               
                 K2C8_MOUSE 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Mus musculus  GN = Krt8 PE = 1 SV = 4 
               
               
                 HBB_CALTO 
               
               
                 Hemoglobin subunit beta OS =  Callicebus torquatus  GN = HBB PE = 2 SV = 3 
               
               
                 K1C3_XENLA 
               
               
                 Keratin, type I cytoskeletal 47 kDa (Fragment) OS =  Xenopus laevis  GN = xk81b1 
               
               
                 PE = 3 SV = 2 
               
               
                 K1C24_HUMAN 
               
               
                 Keratin, type I cytoskeletal 24 OS =  Homo sapiens  GN = KRT24 PE = 1 SV = 1 
               
               
                 K2C4_HUMAN 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Homo sapiens  GN = KRT4 PE = 1 SV = 4 
               
               
                 AATC_HORSE 
               
               
                 Aspartate aminotransferase, cytoplasmic OS =  Equus caballus  GN = GOT1 PE = 1 SV = 2 
               
               
                 K1C16_HUMAN 
               
               
                 Keratin, type I cytoskeletal 16 OS =  Homo sapiens  GN = KRT16 PE = 1 SV = 4 
               
               
                 HBA_HUMAN 
               
               
                 Hemoglobin subunit alpha OS =  Homo sapiens  GN = HBA1 PE = 1 SV = 2 
               
               
                 HBA1_BUBBU 
               
               
                 Hemoglobin subunit alpha-1 OS =  Bubalus bubalis  PE = 2 SV = 3 
               
               
                 K1C42_RAT 
               
               
                 Keratin, type I cytoskeletal 42 OS =  Rattus norvegicus  GN = Krt42 PE = 2 SV = 1 
               
               
                 K1C14_CHICK 
               
               
                 Keratin, type I cytoskeletal 14 OS =  Gallus gallus  GN = KRT14 PE = 2 SV = 1 
               
               
                 HBA_MESAU 
               
               
                 Hemoglobin subunit alpha OS =  Mesocricetus auratus  GN = HBA PE = 1 SV = 1 
               
               
                 UP03_PINHA 
               
               
                 Unknown protein 3 (Fragment) OS =  Pinus halepensis  PE = 1 SV = 1 
               
               
                 IGH1M_MOUSE 
               
               
                 Ig gamma-1 chain C region, membrane-bound form OS =  Mus musculus  GN = Ighg1 
               
               
                 PE = 1 SV = 2 
               
               
                 K2C5_PANTR 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Pan troglodytes  GN = KRT5 PE = 2 SV = 1 
               
               
                 HBA_BISBO 
               
               
                 Hemoglobin subunit alpha-I/II OS =  Bison bonasus  PE = 1 SV = 2 
               
               
                 HBA_TALEU 
               
               
                 Hemoglobin subunit alpha OS =  Talpa europaea  GN = HBA PE = 1 SV = 1 
               
               
                 HIR3_CHAGB 
               
               
                 Histone transcription regulator 3 homolog OS =  Chaetomium globosum  GN = HIR3 
               
               
                 PE = 3 SV = 1 
               
               
                 K1C10_HUMAN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Homo sapiens  GN = KRT10 PE = 1 SV = 4 
               
               
                 K22E_HUMAN 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Homo sapiens  GN = KRT2 PE = 1 SV = 1 
               
               
                 K1C9_HUMAN 
               
               
                 Keratin, type I cytoskeletal 9 OS =  Homo sapiens  GN = KRT9 PE = 1 SV = 2 
               
               
                 K2C1_PANTR 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Pan troglodytes  GN = KRT1 PE = 2 SV = 1 
               
               
                 K2C1_HUMAN 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Homo sapiens  GN = KRT1 PE = 1 SV = 5 
               
               
                 K1C10_CANFA 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Canis familiaris  GN = KRT10 PE = 2 SV = 1 
               
               
                 K1C14_HUMAN 
               
               
                 Keratin, type I cytoskeletal 14 OS =  Homo sapiens  GN = KRT14 PE = 1 SV = 3 
               
               
                 K2C5_PANTR 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Pan troglodytes  GN = KRT5 PE = 2 SV = 1 
               
               
                 K2C6A_HUMAN 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Homo sapiens  GN = KRT6A PE = 1 SV = 3 
               
               
                 K1C16_HUMAN 
               
               
                 Keratin, type I cytoskeletal 16 OS =  Homo sapiens  GN = KRT16 PE = 1 SV = 4 
               
               
                 TRYP_PIG 
               
               
                 Trypsin OS =  Sus scrofa  PE = 1 SV = 1 
               
               
                 K2C79_HUMAN 
               
               
                 Keratin, type II cytoskeletal 79 OS =  Homo sapiens  GN = KRT79 PE = 1 SV = 1 
               
               
                 K1C15_HUMAN 
               
               
                 Keratin, type I cytoskeletal 15 OS =  Homo sapiens  GN = KRT15 PE = 1 SV = 2 
               
               
                 K1C15_SHEEP 
               
               
                 Keratin, type I cytoskeletal 15 OS =  Ovis aries  GN = KRT15 PE = 2 SV = 1 
               
               
                 K1C10_BOVIN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Bos taurus  GN = KRT10 PE = 3 SV = 1 
               
               
                 K1C13_HUMAN 
               
               
                 Keratin, type I cytoskeletal 13 OS =  Homo sapiens  GN = KRT13 PE = 1 SV = 3 
               
               
                 K2CO_CHICK 
               
               
                 Keratin, type II cytoskeletal cochleal OS =  Gallus gallus  PE = 2 SV = 1 
               
               
                 K2C1_RAT 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Rattus norvegicus  GN = Krt1 PE = 2 SV = 1 
               
               
                 K2C5_RAT 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Rattus norvegicus  GN = Krt5 PE = 1 SV = 1 
               
               
                 K2C75_BOVIN 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Bos taurus  GN = KRT75 PE = 2 SV = 1 
               
               
                 K2C7_BOVIN 
               
               
                 Keratin, type II cytoskeletal 7 OS =  Bos taurus  GN = KRT7 PE = 2 SV = 1 
               
               
                 K2C5_BOVIN 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Bos taurus  GN = KRT5 PE = 1 SV = 1 
               
               
                 K2C6A_RAT 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Rattus norvegicus  GN = Krt6a PE = 1 SV = 1 
               
               
                 K2C75_RAT 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Rattus norvegicus  GN = Krt75 PE = 2 SV = 2 
               
               
                 K2C73_MOUSE 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Mus musculus  GN = Krt73 PE = 2 SV = 1 
               
               
                 K22E_CANFA 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Canis familiaris  GN = KRT2 PE = 2 
               
               
                 SV = 1 
               
               
                 K2C4_RAT 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Rattus norvegicus  GN = Krt4 PE = 2 SV = 1 5 
               
               
                 K1C3_XENLA 
               
               
                 Keratin, type I cytoskeletal 47 kDa (Fragment) OS =  Xenopus laevis  GN = xk81b1 
               
               
                 PE = 3 SV = 2 
               
               
                 K22O_HUMAN 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Homo sapiens  GN = KRT76 PE = 1 SV = 1 
               
               
                 K2C3_RABIT 
               
               
                 Keratin, type II cytoskeletal 3 OS =  Oryctolagus cuniculus  GN = KRT3 PE = 2 SV = 1 
               
               
                 K1C19_RAT 
               
               
                 Keratin, type I cytoskeletal 19 OS =  Rattus norvegicus  GN = Krt19 PE = 1 SV = 2 
               
               
                 ALBU_MOUSE 
               
               
                 Serum albumin OS =  Mus musculus  GN = Alb PE = 1 SV = 3 
               
               
                 K2C8_XENLA 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Xenopus laevis  PE = 2 SV = 1 
               
               
                 K2C8_RAT 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Rattus norvegicus  GN = Krt8 PE = 1 SV = 3 
               
               
                 K22O_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Mus musculus  GN = Krt76 PE = 2 SV = 1 
               
               
                 K2C4_HUMAN 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Homo sapiens  GN = KRT4 PE = 1 SV = 4 
               
               
                 TRY1_RAT 
               
               
                 Anionic trypsin-1 OS =  Rattus norvegicus  GN = Prss1 PE = 1 SV = 1 
               
               
                 K1C13_MOUSE 
               
               
                 Keratin, type I cytoskeletal 13 OS =  Mus musculus  GN = Krt13 PE = 2 SV = 2 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Mus musculus  GN = Krt4 PE = 2 SV = 2 
               
               
                 K2C8_DANRE 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Danio rerio  GN = krt8 PE = 1 SV = 1 
               
               
                 KRT85_MOUSE 
               
               
                 Keratin, type II cuticular Hb5 OS =  Mus musculus  GN = Krt85 PE = 2 SV = 2 
               
               
                 K2C80_BOVIN 
               
               
                 Keratin, type II cytoskeletal 80 OS =  Bos taurus  GN = KRT80 PE = 2 SV = 1 
               
               
                 K1C12_RABIT 
               
               
                 Keratin, type I cytoskeletal 12 (Fragment) OS =  Oryctolagus cuniculus   
               
               
                 GN = KRT12 PE = 2 SV = 1 
               
               
                 K1C12_RAT 
               
               
                 Keratin, type I cytoskeletal 12 OS =  Rattus norvegicus  GN = Krt12 PE = 2 SV = 1 
               
               
                 K2C1B_RAT 
               
               
                 Keratin, type II cytoskeletal 1b OS =  Rattus norvegicus  GN = Krt77 PE = 2 SV = 1 
               
               
                 K1C1_XENLA 
               
               
                 Keratin, type I cytoskeletal 47 kDa OS =  Xenopus laevis  GN = xk81a1 PE = 2 SV = 1 
               
               
                 K2C73_HUMAN 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Homo sapiens  GN = KRT73 PE = 1 SV = 1 
               
               
                 K1C24_HUMAN 
               
               
                 Keratin, type I cytoskeletal 24 OS =  Homo sapiens  GN = KRT24 PE = 1 SV = 1 
               
               
                 K2C8_MOUSE 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Mus musculus  GN = Krt8 PE = 1 SV = 4 
               
               
                 K2C78_HUMAN 
               
               
                 Keratin, type II cytoskeletal 78 OS =  Homo sapiens  GN = KRT78 PE = 1 SV = 2 
               
               
                 K2C72_HUMAN 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Homo sapiens  GN = KRT72 PE = 1 SV = 2 
               
               
                 K2C72_MOUSE 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Mus musculus  GN = Krt72 PE = 2 SV = 1 
               
               
                 UP01_PINHA 
               
               
                 Unknown protein 1 (Fragment) OS =  Pinus halepensis  PE = 1 SV = 1 
               
               
                 K22E_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Mus musculus  GN = Krt2 PE = 1 SV = 1 
               
               
                 K1C14_CHICK 
               
               
                 Keratin, type I cytoskeletal 14 OS =  Gallus gallus  GN = KRT14 PE = 2 SV = 1 6 
               
               
                 K1C27_HUMAN 
               
               
                 Keratin, type I cytoskeletal 27 OS =  Homo sapiens  GN = KRT27 PE = 1 SV = 1 
               
               
                 UP03_PINHA 
               
               
                 Unknown protein 3 (Fragment) OS =  Pinus halepensis  PE = 1 SV = 1 
               
               
                 FDL30_ARATH 
               
               
                 Putative F-box/FBD/LRR-repeat protein At5g22610 OS =  Arabidopsis thaliana   
               
               
                 GN = At5g22610 PE = 4 SV = 1 
               
               
                 NDC80_CANAL 
               
               
                 Probable kinetochore protein NDC80 OS =  Candida albicans  GN = NDC80 PE = 3 SV = 1 
               
               
                 K1C10_HUMAN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Homo sapiens  GN = KRT10 PE = 1 SV = 4 
               
               
                 K22E_HUMAN 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Homo sapiens  GN = KRT2 PE = 1 SV = 1 
               
               
                 K2C1_PANTR 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Pan troglodytes  GN = KRT1 PE = 2 SV = 1 
               
               
                 K1C10_CANFA 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Canis familiaris  GN = KRT10 PE = 2 SV = 1 
               
               
                 K1C9_HUMAN 
               
               
                 Keratin, type I cytoskeletal 9 OS =  Homo sapiens  GN = KRT9 PE = 1 SV = 2 
               
               
                 K2C1_HUMAN 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Homo sapiens  GN = KRT1 PE = 1 SV = 5 
               
               
                 K2C75_BOVIN 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Bos taurus  GN = KRT75 PE = 2 SV = 1 
               
               
                 K2C75_RAT 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Rattus norvegicus  GN = Krt75 PE = 2 SV = 2 
               
               
                 K2C1_RAT 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Rattus norvegicus  GN = Krt1 PE = 2 SV = 1 
               
               
                 K2C6A_RAT 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Rattus norvegicus  GN = Krt6a PE = 1 SV = 1 
               
               
                 K2C6A_HUMAN 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Homo sapiens  GN = KRT6A PE = 1 SV = 3 
               
               
                 K2C5_BOVIN 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Bos taurus  GN = KRT5 PE = 1 SV = 1 
               
               
                 K2C5_MOUSE 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Mus musculus  GN = Krt5 PE = 2 SV = 1 
               
               
                 K2C6A_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Mus musculus  GN = Krt6a PE = 2 SV = 3 
               
               
                 K2C79_BOVIN 
               
               
                 Keratin, type II cytoskeletal 79 OS =  Bos taurus  GN = KRT79 PE = 2 SV = 1 
               
               
                 ALBU_MOUSE 
               
               
                 Serum albumin OS =  Mus musculus  GN = Alb PE = 1 SV = 3 
               
               
                 K2C1_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Mus musculus  GN = Krt1 PE = 1 SV = 4 
               
               
                 TRYP_PIG 
               
               
                 Trypsin OS =  Sus scrofa  PE = 1 SV = 1 
               
               
                 K1C10_BOVIN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Bos taurus  GN = KRT10 PE = 3 SV = 1 
               
               
                 K22E_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Mus musculus  GN = Krt2 PE = 1 SV = 1 
               
               
                 HBA_SPAEH 
               
               
                 Hemoglobin subunit alpha OS =  Spalax leucodon ehrenbergi  GN = HBA PE = 1 SV = 1 
               
               
                 HBA_SPECI 
               
               
                 Hemoglobin subunit alpha OS =  Spermophilus citellus  GN = HBA PE = 1 SV = 1 
               
               
                 K2C73_MOUSE 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Mus musculus  GN = Krt73 PE = 2 SV = 1 
               
               
                 K2C1B_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1b OS =  Mus musculus  GN = Krt77 PE = 1 SV = 1 
               
               
                 K2C4_RAT 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Rattus norvegicus  GN = Krt4 PE = 2 SV = 1 
               
               
                 K2C73_HUMAN 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Homo sapiens  GN = KRT73 PE = 1 SV = 1 
               
               
                 K2C5_PANTR 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Pan troglodytes  GN = KRT5 PE = 2 SV = 1 8 
               
               
                 TRY1_RAT 
               
               
                 Anionic trypsin-1 OS =  Rattus norvegicus  GN = Prss1 PE = 1 SV = 1 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Mus musculus  GN = Krt4 PE = 2 SV = 2 
               
               
                 K22O_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Mus musculus  GN = Krt76 PE = 2 SV = 1 
               
               
                 K2C3_HUMAN 
               
               
                 Keratin, type II cytoskeletal 3 OS =  Homo sapiens  GN = KRT3 PE = 1 SV = 2 
               
               
                 K2C72_HUMAN 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Homo sapiens  GN = KRT72 PE = 1 SV = 2 
               
               
                 K2C72_MOUSE 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Mus musculus  GN = Krt72 PE = 2 SV = 1 
               
               
                 HBA_MOUSE 
               
               
                 Hemoglobin subunit alpha OS =  Mus musculus  GN = Hba PE = 1 SV = 2 
               
               
                 K1C14_HUMAN 
               
               
                 Keratin, type I cytoskeletal 14 OS =  Homo sapiens  GN = KRT14 PE = 1 SV = 3 
               
               
                 K1C3_XENLA 
               
               
                 Keratin, type I cytoskeletal 47 kDa (Fragment) OS =  Xenopus laevis  GN = xk81b1 
               
               
                 PE = 3 SV = 2 
               
               
                 K1C1_XENLA 
               
               
                 Keratin, type I cytoskeletal 47 kDa OS =  Xenopus laevis  GN = xk81a1 PE = 2 SV = 1 
               
               
                 K1C12_RABIT 
               
               
                 Keratin, type I cytoskeletal 12 (Fragment) OS =  Oryctolagus cuniculus   
               
               
                 GN = KRT12 PE = 2 SV = 1 
               
               
                 K1C12_RAT 
               
               
                 Keratin, type I cytoskeletal 12 OS =  Rattus norvegicus  GN = Krt12 PE = 2 SV = 1 
               
               
                 K2C8_MOUSE 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Mus musculus  GN = Krt8 PE = 1 SV = 4 
               
               
                 ALBU_FELCA 
               
               
                 Serum albumin OS =  Felis silvestris catus  GN = ALB PE = 1 SV = 1 
               
               
                 K2C4_HUMAN 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Homo sapiens  GN = KRT4 PE = 1 SV = 4 
               
               
                 HBB_CALTO 
               
               
                 Hemoglobin subunit beta OS =  Callicebus torquatus  GN = HBB PE = 2 SV = 3 
               
               
                 K1C25_BOVIN 
               
               
                 Keratin, type I cytoskeletal 25 OS =  Bos taurus  GN = KRT25 PE = 2 SV = 1 
               
               
                 HBA_MESAU 
               
               
                 Hemoglobin subunit alpha OS =  Mesocricetus auratus  GN = HBA PE = 1 SV = 1 
               
               
                 K2C8_BOVIN 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Bos taurus  GN = KRT8 PE = 2 SV = 3 
               
               
                 K2C8_RAT 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Rattus norvegicus  GN = Krt8 PE = 1 SV = 3 
               
               
                 MATK_ADELA 
               
               
                 Maturase K OS =  Adesmia lanata  GN = matK PE = 3 SV = 1 
               
               
                 HBA_BISBO 
               
               
                 Hemoglobin subunit alpha-I/II OS =  Bison bonasus  PE = 1 SV = 2 
               
               
                 HBA_TALEU 
               
               
                 Hemoglobin subunit alpha OS =  Talpa europaea  GN = HBA PE = 1 SV = 1 
               
               
                 K1C10_HUMAN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Homo sapiens  GN = KRT10 PE = 1 SV = 4 
               
               
                 K1C9_HUMAN 
               
               
                 Keratin, type I cytoskeletal 9 OS =  Homo sapiens  GN = KRT9 PE = 1 SV = 2 
               
               
                 K1C10_CANFA 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Canis familiaris  GN = KRT10 PE = 2 SV = 1 
               
               
                 K22E_HUMAN 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Homo sapiens  GN = KRT2 PE = 1 SV = 1 
               
               
                 K2C1_HUMAN 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Homo sapiens  GN = KRT1 PE = 1 SV = 5 
               
               
                 K2C1_PANTR 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Pan troglodytes  GN = KRT1 PE = 2 SV = 1 
               
               
                 CASQ1_MOUSE 
               
               
                 Calsequestrin-1 OS =  Mus musculus  GN = Casq1 PE = 2 SV = 2 
               
               
                 K2C6A_HUMAN 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Homo sapiens  GN = KRT6A PE = 1 SV = 3 
               
               
                 HBB_GORGO 
               
               
                 Hemoglobin subunit beta OS =  Gorilla gorilla gorilla  GN = HBB PE = 1 SV = 2 
               
               
                 K2C5_HUMAN 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Homo sapiens  GN = KRT5 PE = 1 SV = 3 
               
               
                 K2C1_RAT 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Rattus norvegicus  GN = Krt1 PE = 2 SV = 1 
               
               
                 K1C10_BOVIN 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Bos taurus  GN = KRT10 PE = 3 SV = 1 
               
               
                 HBB_MELME 
               
               
                 Hemoglobin subunit beta OS =  Meles meles  GN = HBB PE = 1 SV = 1 
               
               
                 HBB_CALTO 
               
               
                 Hemoglobin subunit beta OS =  Callicebus torquatus  GN = HBB PE = 2 SV = 3 
               
               
                 HBA_HUMAN 
               
               
                 Hemoglobin subunit alpha OS =  Homo sapiens  GN = HBA1 PE = 1 SV = 2 
               
               
                 ALBU_RAT 
               
               
                 Serum albumin OS =  Rattus norvegicus  GN = Alb PE = 1 SV = 2 
               
               
                 ALBU_FELCA 
               
               
                 Serum albumin OS =  Felis silvestris catus  GN = ALB PE = 1 SV = 1 
               
               
                 K2C6A_RAT 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Rattus norvegicus  GN = Krt6a PE = 1 SV = 1 
               
               
                 ALBU_HUMAN 
               
               
                 Serum albumin OS =  Homo sapiens  GN = ALB PE = 1 SV = 2 
               
               
                 TRYP_PIG 
               
               
                 Trypsin OS =  Sus scrofa  PE = 1 SV = 1 
               
               
                 K2C75_BOVIN 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Bos taurus  GN = KRT75 PE = 2 SV = 1 
               
               
                 MLE1_HUMAN 
               
               
                 Myosin light chain 1, skeletal muscle isoform OS =  Homo sapiens  GN = MYL1 PE = 1 
               
               
                 SV = 3 
               
               
                 ACTS_ATRMM 
               
               
                 Actin, alpha skeletal muscle OS =  Atractaspis microlepidota microlepidota   
               
               
                 GN = ACTA1 PE = 2 SV = 1 
               
               
                 ACT2_MOLOC 
               
               
                 Actin, muscle-type OS =  Molgula oculata  PE = 3 SV = 1 
               
               
                 HBB_NASNA 
               
               
                 Hemoglobin subunit beta OS =  Nasua nasua  GN = HBB PE = 1 SV = 1 
               
               
                 HBB_CANFA 
               
               
                 Hemoglobin subunit beta OS =  Canis familiaris  GN = HBB PE = 1 SV = 1 10 
               
               
                 HBA_MESAU 
               
               
                 Hemoglobin subunit alpha OS =  Mesocricetus auratus  GN = HBA PE = 1 SV = 1 
               
               
                 K2C5_BOVIN 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Bos taurus  GN = KRT5 PE = 1 SV = 1 
               
               
                 K2C6A_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Mus musculus  GN = Krt6a PE = 2 SV = 3 
               
               
                 HBB_MACMU 
               
               
                 Hemoglobin subunit beta OS =  Macaca mulatta  GN = HBB PE = 1 SV = 1 
               
               
                 HBB_SAISC 
               
               
                 Hemoglobin subunit beta OS =  Saimiri sciureus  GN = HBB PE = 1 SV = 2 
               
               
                 TRY1_RAT 
               
               
                 Anionic trypsin-1 OS =  Rattus norvegicus  GN = Prss1 PE = 1 SV = 1 
               
               
                 K2C8_MOUSE 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Mus musculus  GN = Krt8 PE = 1 SV = 4 
               
               
                 K2C1B_RAT 
               
               
                 Keratin, type II cytoskeletal 1b OS =  Rattus norvegicus  GN = Krt77 PE = 2 SV = 1 
               
               
                 MDHM_BOVIN 
               
               
                 Malate dehydrogenase, mitochondrial OS =  Bos taurus  GN = MDH2 PE = 2 SV = 1 
               
               
                 HBA_CRIGA 
               
               
                 Hemoglobin subunit alpha OS =  Cricetomys gambianus  GN = HBA PE = 1 SV = 2 
               
               
                 HBA_SPECI 
               
               
                 Hemoglobin subunit alpha OS =  Spermophilus citellus  GN = HBA PE = 1 SV = 1 
               
               
                 HBB_PAGLA 
               
               
                 Hemoglobin subunit beta OS =  Paguma larvata  GN = HBB PE = 1 SV = 1 
               
               
                 HBB_MYOVE 
               
               
                 Hemoglobin subunit beta OS =  Myotis velifer  GN = HBB PE = 1 SV = 1 
               
               
                 K1C1_XENLA 
               
               
                 Keratin, type I cytoskeletal 47 kDa OS =  Xenopus laevis  GN = xk81a1 PE = 2 SV = 1 
               
               
                 ACT18_DICDI 
               
               
                 Actin-18 OS =  Dictyostelium discoideum  GN = act18 PE = 3 SV = 3 
               
               
                 K1C24_HUMAN 
               
               
                 Keratin, type I cytoskeletal 24 OS =  Homo sapiens  GN = KRT24 PE = 1 SV = 1 
               
               
                 ACT1_ARTSX 
               
               
                 Actin, clone 205 OS =  Artemia  sp. PE = 2 SV = 1 
               
               
                 ACTC_BRAFL 
               
               
                 Actin, cytoplasmic OS =  Branchiostoma floridae  PE = 2 SV = 1 
               
               
                 ACTB3_FUGRU 
               
               
                 Actin, cytoplasmic 3 OS =  Fugu rubripes  GN = actbc PE = 2 SV = 1 
               
               
                 ACT_THELA 
               
               
                 Actin OS =  Thermomyces lanuginosus  PE = 3 SV = 1 
               
               
                 ACT1_SACKO 
               
               
                 Actin-1 OS =  Saccoglossus kowalevskii  PE = 2 SV = 1 
               
               
                 ACT1_LYTPI 
               
               
                 Actin, cytoskeletal 1 OS =  Lytechinus pictus  PE = 2 SV = 1 
               
               
                 ACTC_BRALA 
               
               
                 Actin, cytoplasmic OS =  Branchiostoma lanceolatum  PE = 2 SV = 1 
               
               
                 ACT_CANGA 
               
               
                 Actin OS = Candida glabrata GN = ACT1 PE = 3 SV = 1 
               
               
                 ACT_KLULA 
               
               
                 Actin OS =  Kluyveromyces lactis  GN = ACT PE = 3 SV = 2 
               
               
                 ACT_PHARH 
               
               
                 Actin OS =  Phaffia rhodozyma  PE = 3 SV = 1 
               
               
                 ACT_CANAL 
               
               
                 Actin OS =  Candida albicans  GN = ACT1 PE = 3 SV = 1 
               
               
                 ACT_SCEPO 
               
               
                 Actin OS =  Schizosaccharomyces pombe  GN = act1 PE = 1 SV = 1 11 
               
               
                 ACTBL_HUMAN 
               
               
                 Beta-actin-like protein 2 OS =  Homo sapiens  GN = ACTBL2 PE = 1 SV = 2 
               
               
                 A26CA_HUMAN 
               
               
                 ANKRD26-like family C member 1A OS =  Homo sapiens  GN = A26C1A PE = 1 SV = 3 
               
               
                 K2C4_RAT 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Rattus norvegicus  GN = Krt4 PE = 2 SV = 1 
               
               
                 K2C73_HUMAN 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Homo sapiens  GN = KRT73 PE = 1 SV = 1 
               
               
                 HBA_MACFA 
               
               
                 Hemoglobin subunit alpha-A/Q/R/T OS =  Macaca fascicularis  PE = 1 SV = 1 
               
               
                 HBD_GORGO 
               
               
                 Hemoglobin subunit delta OS =  Gorilla gorilla gorilla  GN = HBD PE = 1 SV = 2 
               
               
                 MDHM_YEAST 
               
               
                 Malate dehydrogenase, mitochondrial OS =  Saccharomyces cerevisiae  GN = MDH1 
               
               
                 PE = 1 SV = 2 
               
               
                 HBA_LAMGL 
               
               
                 Hemoglobin subunit alpha OS =  Lama glama  GN = HBA PE = 1 SV = 1 
               
               
                 HBB_RABIT 
               
               
                 Hemoglobin subunit beta-1/2 OS =  Oryctolagus cuniculus  GN = HBB1 PE = 1 SV = 2 
               
               
                 HBD_ELEMA 
               
               
                 Hemoglobin subunit delta OS =  Elephas maximus  GN = HBD PE = 2 SV = 3 
               
               
                 FIBB_HUMAN 
               
               
                 Fibrinogen beta chain OS =  Homo sapiens  GN = FGB PE = 1 SV = 2 
               
               
                 HBD_COLPO 
               
               
                 Hemoglobin subunit delta OS =  Colobus polykomos  GN = HBD PE = 2 SV = 2 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Mus musculus  GN = Krt4 PE = 2 SV = 2 
               
               
                 K22O_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Mus musculus  GN = Krt76 PE = 2 SV = 1 
               
               
                 K2C3_RABIT 
               
               
                 Keratin, type II cytoskeletal 3 OS =  Oryctolagus cuniculus  GN = KRT3 PE = 2 SV = 1 
               
               
                 TPISA_DANRE 
               
               
                 Triosephosphate isomerase A OS =  Danio rerio  GN = tpila PE = 2 SV = 1 
               
               
                 TPIS_ASPOR 
               
               
                 Triosephosphate isomerase OS =  Aspergillus oryzae  GN = tpiA PE = 2 SV = 1 
               
               
                 TPIS_HERAR 
               
               
                 Triosephosphate isomerase OS =  Herminiimonas arsenicoxydans  GN = tpiA PE = 3 SV = 1 
               
               
                 VIM4_XENLA 
               
               
                 Vimentin-4 OS =  Xenopus laevis  GN = vim4 PE = 2 SV = 1 
               
               
                 VIME_BOVIN 
               
               
                 Vimentin OS =  Bos taurus  GN = VIM PE = 1 SV = 3 
               
               
                 MATK_ADELA 
               
               
                 Maturase K OS =  Adesmia lanata  GN = matK PE = 3 SV = 1 
               
               
                 CASA1_BOVIN 
               
               
                 Alpha-S1-casein OS =  Bos taurus  GN = CSN1S1 PE = 1 SV = 2 
               
               
                 MLE1_CHICK 
               
               
                 Myosin light chain 1, skeletal muscle isoform OS =  Gallus gallus  PE = 1 SV = 3 
               
               
                 HBA_ARAAR 
               
               
                 Hemoglobin subunit alpha-A OS =  Ara ararauna  GN = HBAA PE = 1 SV = 2 
               
               
                 PYRB_AERHH 
               
               
                 Aspartate carbamoyltransferase OS =  Aeromonas hydrophila  subsp.  hydrophila   
               
               
                 (strain ATCC 7966/NCIB 9240) GN = pyrB PE = 3 SV = 2 
               
               
                 ATPB_BOVIN 
               
               
                 ATP synthase subunit beta, mitochondrial OS =  Bos taurus  GN = ATP5B PE = 1 SV = 2 
               
               
                 Q8BGZ7_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732475I03 product: CYTOKERATIN homolog (10 days neonate skin cDNA, 
               
               
                 RIKEN full-length enriched library, clone: 4732468K03 product: CYTOKERATIN 
               
               
                 TYPE II homolog) (6 days neonate skin cD 
               
               
                 K2C1_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1 (Cytokeratin-1) (CK-1) (Keratin-1) (K1) (67 kDa 
               
               
                 cytokeratin). -  Mus musculus  (Mouse). 
               
               
                 Q9D2K8_MOUSE 
               
               
                 0 day neonate head cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4833436C19 product: keratin complex 2, basic, gene 1, full insert 
               
               
                 sequence. -  Mus musculus  (Mouse). 
               
               
                 Q32P04_MOUSE 
               
               
                 Krt2-5 protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 K2C6A_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK 6A) (K6a keratin) 
               
               
                 (Keratin-6 alpha) (mK6-alpha). -  Mus musculus  (Mouse). 
               
               
                 Q80VP7_MOUSE 
               
               
                 Hypothetical protein MGC54654. -  Mus musculus  (Mouse). 
               
               
                 Q8BIS2_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732456N10 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D 
               
               
                 (CYTOKERATIN 6D) (CK 6D) (K6D KERATIN). -  Mus musculus  (Mouse). 
               
               
                 Q9CXH5_MOUSE 
               
               
                 17 days embryo head cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 3300001P16 product: hemoglobin, beta adult major chain, full insert 
               
               
                 sequence. -  Mus musculus  (Mouse). 13 
               
               
                 KRMSE1 
               
               
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 Q6NXH9_MOUSE 
               
               
                 Type II keratin Kb36. -  Mus musculus  (Mouse). 
               
               
                 K2C1B_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1b (Type II keratin Kb39) (Embryonic type II 
               
               
                 keratin-1). -  Mus musculus  (Mouse). 
               
               
                 Q6IFZ9_MOUSE 
               
               
                 Type II keratin Kb37. -  Mus musculus  (Mouse). 
               
               
                 Q3TTY5_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732404G19 product: keratin complex 2, basic, gene 17, full insert 
               
               
                 sequence (10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732426A12 product: keratin complex 
               
               
                 Q6IFT3_MOUSE 
               
               
                 Keratin Kb40. -  Mus musculus  (Mouse). 
               
               
                 Q3UV17_MOUSE 
               
               
                 Adult female vagina cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 9930024P18 product: similar to Keratin 2p. -  Mus musculus  (Mouse). 
               
               
                 I59009 
               
               
                 epidermal keratin subunit II - mouse 
               
               
                 HAMS 
               
               
                 hemoglobin alpha chains - mouse 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) 
               
               
                 (Cytoskeletal 57 kDa keratin). -  Mus musculus  (Mouse). 14 
               
               
                 Q6IME9_MOUSE 
               
               
                 Type-II keratin Kb35. -  Mus musculus  (Mouse). 
               
               
                 K2CB_MOUSE 
               
               
                 Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) 
               
               
                 (Cytokeratin endo A). -  Mus musculus  (Mouse). 
               
               
                 Q3TV03_MOUSE 
               
               
                 Adult male stomach cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 2210414C06 product: albumin 1, full insert sequence. -  Mus musculus   
               
               
                 (Mouse). 
               
               
                 Q9Z1R9_MOUSE 
               
               
                 Trypsinogen 16 (Protease, serine, 1). -  Mus musculus  (Mouse). 
               
               
                 JQ0028 
               
               
                 cytokeratin 19 - mouse 
               
               
                 Q2M1G8_MOUSE 
               
               
                 2410039E07Rik protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 AAH11074 
               
               
                 BC011074 NID: -  Mus musculus   
               
               
                 KRMSE1 
               
               
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 Q9CY54_MOUSE 
               
               
                 13 days embryo liver cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 2500004H04 product: hemoglobin, beta adult major chain, full insert 
               
               
                 sequence. -  Mus musculus  (Mouse). 
               
               
                 HAMS 
               
               
                 hemoglobin alpha chains - mouse 
               
               
                 Q9CY06_MOUSE 
               
               
                 13 days embryo liver cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 2510040P05 product: hemoglobin, beta adult major chain, full insert 
               
               
                 sequence. -  Mus musculus  (Mouse). 
               
               
                 AAH11074 
               
               
                 BC011074 NID: -  Mus musculus   
               
               
                 HBMS 
               
               
                 hemoglobin beta major chain - mouse 
               
               
                 Q3TTY5_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732404G19 product: keratin complex 2, basic, gene 17, full insert 
               
               
                 sequence (10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732426A12 product: keratin complex 
               
               
                 Q3UJH8_MOUSE 
               
               
                 16 days embryo heart cDNA, RIKEN full-length enriched library, 
               
               
                 clone: I920001F12 product: glutamate oxaloacetate transaminase 1, soluble, 
               
               
                 full insert sequence. -  Mus musculus  (Mouse). 
               
               
                 Q8BGZ7_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732475I03 product: CYTOKERATIN homolog (10 days neonate skin cDNA, 
               
               
                 RIKEN full-length enriched library, clone: 4732468K03 product: CYTOKERATIN 
               
               
                 TYPE II homolog) (6 days neonate skin cD 16 
               
               
                 K1C17_MOUSE 
               
               
                 Keratin, type I cytoskeletal 17 (Cytokeratin-17) (CK-17) (Keratin-17) 
               
               
                 (K17). -  Mus musculus  (Mouse). 
               
               
                 Q9R0S6_MOUSE 
               
               
                 Beta-1-globin (Fragment). -  Mus musculus  (Mouse). 
               
               
                 K2C1_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1 (Cytokeratin-1) (CK-1) (Keratin-1) (K1) (67 kDa 
               
               
                 cytokeratin). -  Mus musculus  (Mouse). 
               
               
                 Q9D2K8_MOUSE 
               
               
                 0 day neonate head cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4833436C19 product: keratin complex 2, basic, gene 1, full insert 
               
               
                 sequence. -  Mus musculus  (Mouse). 
               
               
                 A55682 
               
               
                 keratin 13, type I cytoskeletal - mouse 
               
               
                 Q32P04_MOUSE 
               
               
                 Krt2-5 protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 CAA33084 
               
               
                 MMHFABP NID: -  Mus musculus   
               
               
                 Q80VP7_MOUSE 
               
               
                 Hypothetical protein MGC54654. -  Mus musculus  (Mouse). 
               
               
                 Q8BIS2_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732456N10 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D 
               
               
                 (CYTOKERATIN 6D) (CK 6D) (K6D KERATIN). -  Mus musculus  (Mouse). 
               
               
                 Q6NXH9_M 
               
               
                 Type II keratin Kb36. -  Mus musculus  (Mouse). 17 
               
               
                 OUSE 
               
               
                 K1C15_MOUSE 
               
               
                 Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) 
               
               
                 (K15). -  Mus musculus  (Mouse). 
               
               
                 FABPH_MOUSE 
               
               
                 Fatty acid-binding protein, heart (H-FABP) (Heart-type fatty acid-binding 
               
               
                 protein) (Mammary-derived growth inhibitor) (MDGI). -  Mus musculus  (Mouse). 
               
               
                 Q3UV17_MOUSE 
               
               
                 Adult female vagina cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 9930024P18 product: similar to Keratin 2p. -  Mus musculus  (Mouse). 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) 
               
               
                 (Cytoskeletal 57 kDa keratin). -  Mus musculus  (Mouse). 
               
               
                 Q6IME9_MOUSE 
               
               
                 Type-II keratin Kb35. -  Mus musculus  (Mouse). 
               
               
                 Q6IFT3_MOUSE 
               
               
                 Keratin Kb40. -  Mus musculus  (Mouse). 
               
               
                 Q8VCW2_MOUSE 
               
               
                 RIKEN cDNA 4631426H08. -  Mus musculus  (Mouse). 
               
               
                 Q2M1G8_MOUSE 
               
               
                 2410039E07Rik protein (Fragment). -  Mus musculus  (Mouse). 
               
               
                 FABPA_MO 
               
               
                 Fatty acid-binding protein, adipocyte (AFABP) (Adipocyte lipid-binding 
               
               
                 protein) (ALBP) (A-FABP) (P2 adipocyte protein) (Myelin P2 protein homolog) 
               
               
                 (3T3-L1 lipid-binding protein) (422 protein) (P15). - 
               
               
                 18 
               
               
                 USE 
               
               
                   Mus musculus  (Mouse). 
               
               
                 I59009 
               
               
                 epidermal keratin subunit II - mouse 
               
               
                 Q3TV03_MOUSE 
               
               
                 Adult male stomach cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 2210414C06 product: albumin 1, full insert sequence. -  Mus musculus   
               
               
                 (Mouse). 
               
               
                 DEMSMC 
               
               
                 malate dehydrogenase (EC 1.1.1.37), cytosolic - mouse 
               
               
                 JQ0028 
               
               
                 cytokeratin 19 - mouse 
               
               
                 Q3UDM1_MOUSE 
               
               
                 Bone marrow macrophage cDNA, RIKEN full-length enriched library, 
               
               
                 clone: G530119K21 product: ATP-binding cassette, sub-family C (CFTR/MRP), 
               
               
                 member 1, full insert sequence. (Fragment). -  Mus musculus  (Mouse). 
               
               
                 Q3UBW7_MOUSE 
               
               
                 Bone marrow macrophage cDNA, RIKEN full-length enriched library, 
               
               
                 clone: I830015F18 product: transferrin, full insert sequence. -  Mus musculus   
               
               
                 (Mouse). 
               
               
                 K1C10_MOUSE 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Mus musculus  GN = Krt10 PE = 1 SV = 3 
               
               
                 K2C75_MOUSE 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Mus musculus  GN = Krt75 PE = 1 SV = 1 
               
               
                 ALBU_MOUSE 
               
               
                 Serum albumin OS =  Mus musculus  GN = Alb PE = 1 SV = 3 
               
               
                 K2C5_MOUSE 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Mus musculus  GN = Krt5 PE = 2 SV = 1 
               
               
                 K2C1_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Mus musculus  GN = Krt1 PE = 1 SV = 4 
               
               
                 K2C6A_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6A OS =  Mus musculus  GN = Krt6a PE = 2 SV = 3 
               
               
                 K2C6B_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6B OS =  Mus musculus  GN = Krt6b PE = 2 SV = 3 
               
               
                 K2C79_MOUSE 
               
               
                 Keratin, type II cytoskeletal 79 OS =  Mus musculus  GN = Krt79 PE = 2 SV = 2 
               
               
                 K1C13_MOUSE 
               
               
                 Keratin, type I cytoskeletal 13 OS =  Mus musculus  GN = Krt13 PE = 2 SV = 2 
               
               
                 AATC_MOUSE 
               
               
                 Aspartate aminotransferase, cytoplasmic OS =  Mus musculus  GN = Got1 PE = 1 SV = 2 
               
               
                 K1C14_MOUSE 
               
               
                 Keratin, type I cytoskeletal 14 OS =  Mus musculus  GN = Krt14 PE = 1 SV = 2 
               
               
                 K1C17_MOUSE 
               
               
                 Keratin, type I cytoskeletal 17 OS =  Mus musculus  GN = Krt17 PE = 1 SV = 3 
               
               
                 K2C73_MOUSE 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Mus musculus  GN = Krt73 PE = 2 SV = 1 
               
               
                 HBB1_MOUSE 
               
               
                 Hemoglobin subunit beta-1 OS =  Mus musculus  GN = Hbb-b1 PE = 1 SV = 2 
               
               
                 K2C71_MOUSE 
               
               
                 Keratin, type II cytoskeletal 71 OS =  Mus musculus  GN = Krt71 PE = 1 SV = 1 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Mus musculus  GN = Krt4 PE = 2 SV = 2 
               
               
                 K22O_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Mus musculus  GN = Krt76 PE = 2 SV = 1 
               
               
                 K2C72_MOUSE 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Mus musculus  GN = Krt72 PE = 2 SV = 1 
               
               
                 K2C8_MOUSE 
               
               
                 Keratin, type II cytoskeletal 8 OS =  Mus musculus  GN = Krt8 PE = 1 SV = 4 
               
               
                 FABPH_MOUSE 
               
               
                 Fatty acid-binding protein, heart OS =  Mus musculus  GN = Fabp3 PE = 1 SV = 5 
               
               
                 SOX1_MOUSE 
               
               
                 SOX-1 protein OS =  Mus musculus  GN = Sox1 PE = 2 SV = 1 
               
               
                 SPA3M_MOUSE 
               
               
                 Serine protease inhibitor A3M OS =  Mus musculus  GN = Serpina3m PE = 1 SV = 1 
               
               
                 TPIS_MOUSE 
               
               
                 Triosephosphate isomerase OS =  Mus musculus  GN = Tpi1 PE = 1 SV = 3 
               
               
                 K1C15_MOUSE 
               
               
                 Keratin, type I cytoskeletal 15 OS =  Mus musculus  GN = Krt15 PE = 1 SV = 2 
               
               
                 K1C16_MOUSE 
               
               
                 Keratin, type I cytoskeletal 16 OS =  Mus musculus  GN = Krt16 PE = 1 SV = 3 
               
               
                 K22E_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Mus musculus  GN = Krt2 PE = 1 SV = 1 
               
               
                 20 
               
               
                 DNJC1_MOUSE 
               
               
                 DnaJ homolog subfamily C member 1 OS =  Mus musculus  GN = Dnajc1 PE = 1 SV = 1 
               
               
                 HBA_MOUSE 
               
               
                 Hemoglobin subunit alpha OS =  Mus musculus  GN = Hba PE = 1 SV = 2 
               
               
                 ARVC_MOUSE 
               
               
                 Armadillo repeat protein deleted in velo-cardio-facial syndrome homolog 
               
               
                 OS =  Mus musculus  GN = Arvcf PE = 1 SV = 2 
               
               
                 VIME_MOUSE 
               
               
                 Vimentin OS =  Mus musculus  GN = Vim PE = 1 SV = 3 
               
               
                 KRT83_MOUSE 
               
               
                 Keratin, type II cuticular Hb3 OS =  Mus musculus  GN = Krt83 PE = 2 SV = 2 
               
               
                 KRT82_MOUSE 
               
               
                 Keratin, type II cuticular Hb2 OS =  Mus musculus  GN = Krt82 PE = 2 SV = 1 
               
               
                 A1AT1_MOUSE 
               
               
                 Alpha-1-antitrypsin 1-1 OS =  Mus musculus  GN = Serpina1a PE = 1 SV = 4 
               
               
                 SODC_MOUSE 
               
               
                 Superoxide dismutase [Cu- Zn] OS =  Mus musculus  GN = Sod1 PE = 1 SV = 2 
               
               
                 AS250_MOUSE 
               
               
                 250 kDa substrate of Akt OS =  Mus musculus  GN = Kiaa1272 PE = 1 SV = 2 
               
               
                 K1C25_MOUSE 
               
               
                 Keratin, type I cytoskeletal 25 OS =  Mus musculus  GN = Krt25 PE = 1 SV = 1 
               
               
                 HBB1_MOUSE 
               
               
                 Hemoglobin subunit beta-1 OS =  Mus musculus  GN = Hbb-b1 PE = 1 SV = 2 
               
               
                 K1C10_MOUSE 
               
               
                 Keratin, type I cytoskeletal 10 OS =  Mus musculus  GN = Krt10 PE = 1 SV = 3 
               
               
                 K2C75_MOUSE 
               
               
                 Keratin, type II cytoskeletal 75 OS =  Mus musculus  GN = Krt75 PE = 1 SV = 1 
               
               
                 K2C1_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1 OS =  Mus musculus  GN = Krt1 PE = 1 SV = 4 
               
               
                 K2C5_MOUSE 
               
               
                 Keratin, type II cytoskeletal 5 OS =  Mus musculus  GN = Krt5 PE = 2 SV = 1 
               
               
                 ALBU_MOUSE 
               
               
                 Serum albumin OS =  Mus musculus  GN = Alb PE = 1 SV = 3 
               
               
                 K2C79_MOUSE 
               
               
                 Keratin, type II cytoskeletal 79 OS =  Mus musculus  GN = Krt79 PE = 2 SV = 2 
               
               
                 K1C42_MOUSE 
               
               
                 Keratin, type I cytoskeletal 42 OS =  Mus musculus  GN = Krt42 PE = 1 SV = 1 
               
               
                 HBA_MOUSE 
               
               
                 Hemoglobin subunit alpha OS =  Mus musculus  GN = Hba PE = 1 SV = 2 
               
               
                 K2C73_MOUSE 
               
               
                 Keratin, type II cytoskeletal 73 OS =  Mus musculus  GN = Krt73 PE = 2 SV = 1 
               
               
                 K1C15_MOUSE 
               
               
                 Keratin, type I cytoskeletal 15 OS =  Mus musculus  GN = Krt15 PE = 1 SV = 2 
               
               
                 K2C1B_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1b OS =  Mus musculus  GN = Krt77 PE = 1 SV = 1 
               
               
                 K1C27_MOUSE 
               
               
                 Keratin, type I cytoskeletal 27 OS =  Mus musculus  GN = Krt27 PE = 1 SV = 1 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 OS =  Mus musculus  GN = Krt4 PE = 2 SV = 2 
               
               
                 K22O_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 oral OS =  Mus musculus  GN = Krt76 PE = 2 SV = 1 
               
               
                 K2C72_MOUSE 
               
               
                 Keratin, type II cytoskeletal 72 OS =  Mus musculus  GN = Krt72 PE = 2 SV = 1 
               
               
                 K1C19_MOUSE 
               
               
                 Keratin, type I cytoskeletal 19 OS =  Mus musculus  GN = Krt19 PE = 2 SV = 1 
               
               
                 K1C17_MOUSE 
               
               
                 Keratin, type I cytoskeletal 17 OS =  Mus musculus  GN = Krt17 PE = 1 SV = 3 
               
               
                 K22E_MOUSE 
               
               
                 Keratin, type II cytoskeletal 2 epidermal OS =  Mus musculus  GN = Krt2 PE = 1 SV = 1 
               
               
                 SYTC_MOUSE 
               
               
                 Threonyl-tRNA synthetase, cytoplasmic OS =  Mus musculus  GN = Tars PE = 1 SV = 2 
               
               
                 HBE_MOUSE 
               
               
                 Hemoglobin subunit epsilon-Y2 OS =  Mus musculus  GN = Hbb-y PE = 1 SV = 2 
               
               
                 FTSJ2_MOUSE 
               
               
                 FtsJ methyltransferase domain-containing protein 2 OS =  Mus musculus   
               
               
                 GN = Ftsjd2 PE = 1 SV = 1 
               
               
                 DPOLA_MOUSE 
               
               
                 DNA polymerase alpha catalytic subunit OS =  Mus musculus  GN = Pola1 PE = 1 SV = 2 
               
               
                 HBB1_MOUSE Mass: 15830 Score: 216 Queries matched: 10 emPAI: 2.20 
               
               
                 Hemoglobin subunit beta-1 OS =  Mus musculus  GN = Hbb-b1 PE = 1 SV = 2 
               
               
                 Check to include this hit in error tolerant search or archive report 
               
               
                 HAMS 
               
               
                 hemoglobin alpha chains - mouse 
               
               
                 Q9CY12_MOUSE 
               
               
                 13 days embryo liver cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 2510039D09 product: hemoglobin, beta adult major chain, full insert 
               
               
                 sequence.-  Mus musculus  (Mouse). 
               
               
                 Q8BGZ7_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732475I03 product: CYTOKERATIN homolog (10 days neonate skin cDNA, 
               
               
                 RIKEN full-length enriched library, clone: 4732468K03 product: CYTOKERATIN 
               
               
                 TYPE II homolog) (6 days neonate skin cD 
               
               
                 Q32P04_MOUSE 
               
               
                 Krt2-5 protein (Fragment).-  Mus musculus  (Mouse). 
               
               
                 Q3TV03_MOUSE 
               
               
                 Adult male stomach cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 2210414C06 product: albumin 1, full insert sequence.-  Mus musculus   
               
               
                 (Mouse). 
               
               
                 KRMSE1 
               
               
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 K1C15_MOUSE 
               
               
                 Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) 
               
               
                 (K15).-  Mus musculus  (Mouse). 
               
               
                 Q8BIS2_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732456N10 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D 
               
               
                 (CYTOKERATIN 6D) (CK 6D) (K6D KERATIN).-  Mus musculus  (Mouse). 
               
               
                 Q8BGZ7_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732475I03 product: CYTOKERATIN homolog (10 days neonate skin cDNA, 
               
               
                 RIKEN full-length enriched library, clone: 4732468K03 product: CYTOKERATIN 
               
               
                 TYPE II homolog) (6 days neonate skin cD 
               
               
                 K2C6A_MOUSE 
               
               
                 Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK 6A) (K6a keratin) 
               
               
                 (Keratin-6 alpha) (mK6-alpha).-  Mus musculus  (Mouse). 
               
               
                 Q32P04_MOUSE 
               
               
                 Krt2-5 protein (Fragment).-  Mus musculus  (Mouse). 
               
               
                 Q8K2E4_MOUSE 
               
               
                 CDNA sequence BC031593.-  Mus musculus  (Mouse). 
               
               
                 K2C1_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1 (Cytokeratin-1) (CK-1) (Keratin-1) (K1) (67 kDa 
               
               
                 cytokeratin).-  Mus musculus  (Mouse). 
               
               
                 Q9D2K8_MOUSE 
               
               
                 0 day neonate head cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4833436C19 product: keratin complex 2, basic, gene 1, full insert 
               
               
                 sequence.-  Mus musculus  (Mouse). 
               
               
                 Q8BIS2_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732456N10 product: similar to KERATIN, TYPE II CYTOSKELETAL 6D 
               
               
                 (CYTOKERATIN 6D) (CK 6D) (K6D KERATIN).-  Mus musculus  (Mouse). 
               
               
                 Q80VP7_MOUSE 
               
               
                 Hypothetical protein MGC54654.-  Mus musculus  (Mouse). 
               
               
                 Q6NXH9_MOUSE 
               
               
                 Type II keratin Kb36.-  Mus musculus  (Mouse). 
               
               
                 Q6IFT3_MO 
               
               
                 Keratin Kb40.-  Mus musculus  (Mouse). 25 
               
               
                 USE 
               
               
                 K2C1B_MOUSE 
               
               
                 Keratin, type II cytoskeletal 1b (Type II keratin Kb39) (Embryonic type II 
               
               
                 keratin-1).-  Mus musculus  (Mouse). 
               
               
                 Q3UV17_MOUSE 
               
               
                 Adult female vagina cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 9930024P18 product: similar to Keratin 2p.-  Mus musculus  (Mouse). 
               
               
                 K2C4_MOUSE 
               
               
                 Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) 
               
               
                 (Cytoskeletal 57 kDa keratin).-  Mus musculus  (Mouse). 
               
               
                 AAH11074 
               
               
                 BC011074 NID: -  Mus musculus   
               
               
                 BAA85657 
               
               
                 AB033744 NID: -  Mus musculus   
               
               
                 Q6IFZ9_MOUSE 
               
               
                 Type II keratin Kb37.-  Mus musculus  (Mouse). 
               
               
                 I59009 
               
               
                 epidermal keratin subunit II - mouse 
               
               
                 K1C17_MOUSE 
               
               
                 Keratin, type I cytoskeletal 17 (Cytokeratin-17) (CK-17) (Keratin-17) 
               
               
                 (K17).-  Mus musculus  (Mouse). 
               
               
                 Q6IFX2_MOUSE 
               
               
                 Type I keratin KA22.-  Mus musculus  (Mouse). 
               
               
                 Q6IME9_MOUSE 
               
               
                 Type-II keratin Kb35.-  Mus musculus  (Mouse). 
               
               
                 BAE40567 
               
               
                 AK168726 NID: -  Mus musculus   
               
               
                 AAD01692 
               
               
                 AF021836 NID: -  Mus musculus   
               
               
                 Q3USS4 
               
               
                 Adult male corpora quadrigemina cDNA, RIKEN full-length enriched library, 
               
               
                 clone: B230315D14 product: glial fibrillary acidic protein, full insert 
               
               
                 sequence.-  Mus musculus  (Mouse). 26 
               
               
                 _MOUSE 
               
               
                 Q3TTY5_MOUSE 
               
               
                 10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732404G19 product: keratin complex 2, basic, gene 17, full insert 
               
               
                 sequence (10 days neonate skin cDNA, RIKEN full-length enriched library, 
               
               
                 clone: 4732426A12 product: keratin complex 
               
               
                 K1C15_MOUSE 
               
               
                 Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) 
               
               
                 (K15).-  Mus musculus  (Mouse). 
               
               
                 Q3TWV0_MOUSE 
               
               
                 Osteoclast-like cell cDNA, RIKEN full-length enriched library, 
               
               
                 clone: I420023H06 product: vimentin, full insert sequence.-  Mus musculus   
               
               
                 (Mouse). 
               
               
                 Q9JKB4_MOUSE 
               
               
                 Epidermal keratin 10 (Fragment).-  Mus musculus  (Mouse). 
               
               
                 Q8VCW2_MOUSE 
               
               
                 RIKEN cDNA 4631426H08.-  Mus musculus  (Mouse). 
               
               
                 JQ0028 
               
               
                 cytokeratin 19 - mouse 
               
               
                 KRMSE1 
               
               
                 keratin, 59K type I cytoskeletal - mouse 
               
               
                 JC4030 
               
               
                 DnaJ-like protein MTJ1 - mouse 
               
               
                 E2AK4_MOUSE 
               
               
                 Eukaryotic translation initiation factor 2-alpha kinase 4 (EC 2.7.11.1) 
               
               
                 (GCN2-like protein) (mGCN2).-  Mus musculus  (Mouse). 
               
               
                 BAB27580 
               
               
                 AK011380 NID: -  Mus musculus