Abstract:
Disclosed are novel G-CSF receptor agonist proteins, DNAs which encode the multi-functional hematopoietic receptor agonists proteins, methods of making the multi-functional hematopoietic receptor agonists proteins and methods of using the multi-functional hematopoietic receptor agonists proteins.

Description:
The present application is a divisional of application Ser. No. 08/833,167 filed on Apr. 4, 1997, now U.S. Pat. No. 6,100,070, which is a Continuation in Part of Ser. No. PCT/US96/15935, filed Oct. 4, 1996 and which claims priority under 35 U.S.C. §119(e) of U.S. provisional application Ser. No. 60/004,382 filed Sep. 27, 1995. The noted applications are incorporated herein by reference. 
    
    
     FIELD OF THE INVENTION 
     The present invention relates to human G-CSF receptor agonists with activity on hematopoietic cell differentiation and expansion. 
     BACKGROUND OF THE INVENTION 
     The human blood-forming (hematopoietic) system replaces a variety of white blood cells (including neutrophils, macrophages, and basophils/mast cells), red blood cells (erythrocytes) and clot-forming cells (megakaryocytes/platelets). The hematopoietic systems of the average male has been estimated to produce on the order of 4.5×10 11  granulocytes and erythrocytes every year, which is equivalent to an annual replacement of total body weight (Dexter et al.,  BioEssays,  2;154-158, 1985). 
     It is believed that small amounts of certain hematopoietic growth factors account for the differentiation of a small number of progenitor “stem cells” into the variety of blood cell lines, for the tremendous proliferation of those lines, and for the ultimate differentiation of mature blood cells from those lines. Because the hematopoietic growth factors are present in extremely small amounts, the detection and identification of these factors has relied upon an array of assays which as yet only distinguish among the different factors on the basis of stimulative effects on cultured cells under artificial conditions. 
     U.S. Pat. No. 4,999,291 discloses DNA and methods for making G-CSF the disclosure of which is incorporated herein by reference in it entirety. 
     U.S. Pat. No. 4,810,643 relates to DNA and methods of making G-CSF and Cys to Ser substitution variants of G-CSF. 
     Kuga et al. ( Biochem.+Biophys. Res. Comm.  159:103-111, 1989) made a series of G-CSF variants to partially define the structure-function relationship. Kuga et al. found that internal and C-terminal deletions abolished activity, while N-terminal deletions of up to 11 amino acids and amino acid substitutions at positions 1, 2 and 3 were active. 
     Watanabe et al. ( Anal. Biochem.  195:38-44, 1991) made a variant to study G-CSF receptor binding in which amino acids 1 and 3 were changed to Tyr for radioiodination of the protein. Watanabe et al. found this Tyr 1 , Tyr 3  G-CSF variant to be active. 
     WO 95/27732 describes, but does not show that the molecule has biological activity, a circularly permuted G-CSF ligand with a breakpoint at positions 68/69 creating a circularly permuted G-CSF ligand with a new N-terminus at the original position 69 of G-CSF and a new C-terminus at the original position 68 of G-CSF. WO 95/27732 also discloses circularly permuted GM-CSF, IL-2 and IL-4. 
     Rearrangement of Protein Sequences 
     In evolution, rearrangements of DNA sequences serve an important role in generating a diversity of protein structure and function. Gene duplication and exon shuffling provide an important mechanism to rapidly generate diversity and thereby provide organisms with a competitive advantage, especially since the basal mutation rate is low (Doolittle,  Protein Science  1:191-200, 1992). 
     The development of recombinant DNA methods has made it possible to study the effects of sequence transposition on protein folding, structure and function. The approach used in creating new sequences resembles that of naturally occurring pairs of proteins that are related by linear reorganization of their amino acid sequences (Cunningham, et al.,  Proc. Natl. Acad. Sci. U.S.A.  76:3218-3222, 1979; Teather &amp; Erfle,  J. Bacteriol.  172: 3837-3841, 1990; Schimming et al.,  Eur. J. Biochem.  204: 13-19, 1992; Yamiuchi and Minamikawa,  FEBS Lett.  260:127-130, 1991: MacGregor et al.,  FEBS Lett.  378:263-266, 1996). The first in vitro application of this type of rearrangement to proteins was described by Goldenberg and Creighton ( J. Mol. Biol.  165:407-413, 1983). A new N-terminus is selected at an internal site (breakpoint) of the original sequence, the new sequence having the same order of amino acids as the original from the breakpoint until it reaches an amino acid that is at or near the original C-terminus. At this point the new sequence is joined, either directly or through an additional portion of sequence (linker), to an amino acid that is at or near the original N-terminus, and the new sequence continues with the same sequence as the original until it reaches a point that is at or near the amino acid that was N-terminal to the breakpoint site of the original sequence, this residue forming the new C-terminus of the chain. 
     This approach has been applied to proteins which range in size from 58 to 462 amino acids (Goldenberg &amp; Creighton,  J. Mol. Biol.  165:407-413, 1983; Li &amp; Coffino,  Mol. Cell. Biol.  13:2377-2383, 1993). The proteins examined have represented a broad range of structural classes, including proteins that contain predominantly α-helix (interleukin-4; Kreitman et al., Cytokine 7:311-318, 1995), β-sheet (interleukin-1; Horlick et al.,  Protein Eng.  5:427-431, 1992), or mixtures of the two (yeast phosphoribosyl anthranilate isomerase; Luger et al.,  Science  243:206-210, 1989). Broad categories of protein function are represented in these sequence reorganization studies: 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Enzymes 
                   
               
               
                   
                 T4 lysozyme 
                 Zhang et al., Biochemistry 
               
               
                   
                   
                 32:12311-12318 (1993); Zhang et 
               
               
                   
                   
                 al., Nature Struct. Biol. 1:434-438 
               
               
                   
                   
                 (1995) 
               
               
                   
                 dihydrofolate 
                 Buchwalder et al., Biochemistry 
               
               
                   
                 reductase 
                 31:1621-1630 (1994); Protasova et 
               
               
                   
                   
                 al., Prot. Eng. 7:1373-1377 (1995) 
               
               
                   
                 ribonuclease T1 
                 Mullins et al., J. Am. Chem. Soc. 
               
               
                   
                   
                 116:5529-5533 (1994); Garrett et al., 
               
               
                   
                   
                 Protein Science 5:204-211 (1996) 
               
               
                   
                 Bacillus β-glucanse 
                 Hahn et al., Proc. Natl. Acad. Sci. 
               
               
                   
                   
                 U.S.A. 91:10417-10421 (1994) 
               
               
                   
                 aspartate 
                 Yang &amp; Schachman, Proc. Natl. Acad. 
               
               
                   
                 transcarbamoylase 
                 Sci. U.S.A. 90:11980-11984 (1993) 
               
               
                   
                 phosphoribosyl 
                 Luger et al., Science 243:206-210 
               
               
                   
                 anthranilate 
                 (1989); Luger et al., Prot. Eng. 
               
               
                   
                 isomerase 
                 3:249-258 (1990) 
               
               
                   
                 pepsin/pepsinogen 
                 Lin et al., Protein Science 4:159- 
               
               
                   
                   
                 166 (1995) 
               
               
                   
                 glyceraldehyde-3- 
                 Vignais et al., Protein Science 
               
               
                   
                 phosphate dehydro- 
                 4:994-1000 (1995) 
               
               
                   
                 genase 
               
               
                   
                 ornithine 
                 Li &amp; Coffino, Mol. Cell. Biol. 
               
               
                   
                 decarboxylase 
                 13:2377-2383 (1993) 
               
               
                   
                 yeast 
                 Ritco-Vonsovici et al., Biochemistry 
               
               
                   
                 phosphoglycerate 
                 34:16543-16551 (1995) 
               
               
                   
                 dehydrogenase 
               
               
                   
                 Enzyme Inhibitor 
               
               
                   
                 basic pancreatic 
                 Goldenberg &amp; Creighton, J. Mol. 
               
               
                   
                 trypsin inhibitor 
                 Biol. 165:407-413 (1983) 
               
               
                   
                 Cytokines 
               
               
                   
                 interleukin-1β 
                 Horlick et al., Protein Eng. 5:427- 
               
               
                   
                   
                 431 (1992) 
               
               
                   
                 interleukin-4 
                 Kreitman et al., Cytokine 7:311- 
               
               
                   
                   
                 318 (1995) 
               
               
                   
                 Tyrosine Kinase 
               
               
                   
                 Recognition Domain 
               
               
                   
                 α-spectrin SH3 
                 Viguera, et al., J. 
               
               
                   
                 domain 
                 Mol. Biol. 247:670-681 (1995) 
               
               
                   
                 Transmembrane 
               
               
                   
                 Protein 
               
               
                   
                 omp A 
                 Koebnik &amp; Krämer, J. Mol. Biol. 
               
               
                   
                   
                 250:617-626 (1995) 
               
               
                   
                 Chimeric Protein 
               
               
                   
                 interleukin-4- 
                 Kreitman et al., Proc. Natl. Acad. 
               
               
                   
                 Pseudomonas 
                 Sci. U.S.A. 91:6889-6893 (1994). 
               
               
                   
                 exotoxin fusion 
               
               
                   
                 molecule 
               
               
                   
                   
               
             
          
         
       
     
     The results of these studies have been highly variable. In many cases substantially lower activity, solubility or thermodynamic stability were observed ( E. coli  dihydrofolate reductase, aspartate transcarbamoylase, phosphoribosyl anthranilate isomerase, glyceraldehyde-3-phosphate dehydrogenase, ornithine decarboxylase, omp A, yeast phosphoglycerate dehydrogenase). In other cases, the sequence rearranged protein appeared to have many nearly identical properties as its natural counterpart (basic pancreatic trying inhibitor, T4 lysozyme, ribonuclease T1, Bacillus-βglucanase, interleukin-1β α-spectrin SH3 domain, pepsinogen, interleukin-4). In exceptional cases, an unexpected improvement over some properties of the natural sequence was observed, e.g., the solubility and refolding rate for rearranged α-spectrin SH3 domain sequences, and the receptor affinity and anti-tumor activity of transposed interleukin-4-Pseudomonas exotoxin fusion molecule (Kreitman et al.,  Proc. Natl. Acad. Sci. U.S.A.  91:6889-6893, 1994; Kreitman et al., Cancer Res. 55:3357-3363, 1995). 
     The primary motivation for these types of studies has been to study the role of short-range and long-range interactions in protein folding and stability. Sequence rearrangements of this type convert a subset of interactions that are long-range in the original sequence into short-range interactions in the new sequence, and vice versa. The fact that many of these sequence rearrangements are able to attain a conformation with at least some activity is persuasive evidence that protein folding occurs by multiple folding pathways (Viguera, et al.,  J. Mol. Biol.  247:670-681, 1995). In the case of the SH3 domain of α-spectrin, choosing new termini at locations that corresponded to β-hairpin turns resulted in proteins with slightly less stability, but which were nevertheless able to fold. 
     The positions of the internal breakpoints used in the studies cited here are found exclusively on the surface of proteins, and are distributed throughout the linear sequence without any obvious bias towards the ends or the middle (the variation in the relative distance from the original N-terminus to the breakpoint is ca. 10 to 80% of the total sequence length). The linkers connecting the original N- and C-termini in these studies have ranged from 0 to 9 residues. In one case (Yang &amp; Schachman,  Proc. Natl. Acad. Sci. U.S.A.  90:11980-11984, 1993), a portion of sequence has been deleted from the original C-terminal segment, and the connection made from the truncated C-terminus to the original N-terminus. Flexible hydrophilic residues such as Gly and Ser are frequently used in the linkers. Viguera, et al.( J. Mol. Biol.  247:670-681, 1995) compared joining the original N- and C- termini with 3- or 4-residue linkers; the 3-residue linker was less thermodynamically stable. Protasova et al. ( Protein Eng.  7:1373-1377, 1994) used 3- or 5-residue linkers in connecting the original N-termini of  E. coli  dihydrofolate reductase; only the 3-residue linker produced protein in good yield. 
     SUMMARY OF THE INVENTION 
     The modified human G-CSF receptor agonists of the present invention can be represented by the Formula: 
     
       
         X 1 —(L) a —X 2   
       
     
     wherein; 
     a is 0 or 1; 
     X 1  is a peptide comprising an amino acid sequence corresponding to the sequence of residues n+1 through J; 
     X 2  is a peptide comprising an amino acid sequence corresponding to the sequence of residues 1 through n; 
     n is an integer ranging from 1 to J-1; and 
     L is a linker. 
     In the formula above the constituent amino acids residues of human G-CSF are numbered sequentially 1 through J from the amino to the carboxyl terminus. A pair of adjacent amino acids within this protein may be numbered n and n+1 respectively where n is an integer ranging from 1 to J-1. The residue n+1 becomes the new N-terminus of the new G-CSF receptor agonist and the residue n becomes the new C-terminus of the new G-CSF receptor agonist. 
     The present invention relates to novel G-CSF receptor agonists of the following formula: 
     
       
         
               
               
               
             
           
               
                 1                                   10 
                 (SEQ ID NO:1) 
                   
               
               
                 Xaa Xaa Xaa Gly Pro Ala Ser Ser Leu Pro Gln Ser Xaa 
               
               
                   
               
               
                                         20 
               
               
                 Leu Leu Xaa Xaa Xaa Glu Gln Val Xaa Lys Xaa Gln Gly Xaa Gly 
               
               
                   
               
               
                     30                                      40 
               
               
                 Ala Xaa Leu Gln Glu Xaa Leu Xaa Ala Thr Tyr Lys Leu Xaa Xaa 
               
               
                   
               
               
                                         50 
               
               
                 Xaa Glu Xaa Xaa Val Xaa Xaa Gly His Ser Xaa Gly Ile Pro Trp 
               
               
                   
               
               
                     60                                      70 
               
               
                 Ala Pro Leu Ser Ser Xaa Pro Ser Xaa Ala Leu Xaa Leu Ala Gly 
               
               
                   
               
               
                                         80 
               
               
                 Xaa Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu 
               
               
                   
               
               
                     90                                      100 
               
               
                 Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu 
               
               
                   
               
               
                                         110 
               
               
                 Xaa Thr Leu Gln Xaa Asp Val Ala Asp Phe Ala Xaa Thr Ile Trp 
               
               
                   
               
               
                     120                                     130 
               
               
                 Gln Gln Met Glu Xaa Xaa Gly Met Ala Pro Ala Leu Gln Pro Thr 
               
               
                   
               
               
                                         140 
               
               
                 Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Xaa Gln Xaa Xaa Ala 
               
               
                   
               
               
                     150                                     160 
               
               
                 Gly Gly Val Leu Val Ala Ser Xaa Leu Gln Xaa Phe Leu Xaa Xaa 
               
               
                   
               
               
                                         170 
               
               
                 Ser Tyr Arg Val Leu Xaa Xaa Leu Ala Gln Pro 
               
             
          
         
       
     
     wherein 
     Xaa at position 1 is Thr, Ser, Arg, Tyr or Gly; 
     Xaa at position 2 is Pro or Leu; 
     Xaa at position 3 is Leu, Arg, Tyr or Ser; 
     Xaa at position 13 is Phe, Ser, His, Thr or Pro; 
     Xaa at position 16 is Lys, Pro, Ser, Thr or His; 
     Xaa at position 17 is Cys, Ser, Gly, Ala, Ile, Tyr or Arg; 
     Xaa at position 18 is Leu, Thr, Pro, His, Ile or Cys; 
     Xaa at position 22 is Arg, Tyr, Ser, Thr or Ala; 
     Xaa at position 24 is Ile, Pro, Tyr or Leu; 
     Xaa at position 27 is Asp, or Gly; 
     Xaa at position 30 is Ala, Ile, Leu or Gly; 
     Xaa at position 34 is Lys or Ser; 
     Xaa at position 36 is Cys or Ser; 
     Xaa at position 42 is Cys or Ser; 
     Xaa at position 43 is His, Thr, Gly, Val, Lys, Trp, Ala, Arg, Cys, or Leu; 
     Xaa at position 44 is Pro, Gly, Arg, Asp, Val, Ala, His, Trp, Gln, or Thr; 
     Xaa at position 46 is Glu, Arg, Phe, Arg, Ile or Ala; 
     Xaa at position 47 is Leu or Thr; 
     Xaa at position 49 is Leu, Phe, Arg or Ser; 
     Xaa at position 50 is Leu, Ile, His, Pro or Tyr; 
     Xaa at position 54 is Leu or His; 
     Xaa at position 64 is Cys or Ser; 
     Xaa at position 67 is Gln, Lys, Leu or Cys; 
     Xaa at position 70 is Gln, Pro, Leu, Arg or Ser; 
     Xaa at position 74 is Cys or Ser; 
     Xaa at position 104 is Asp, Gly or Val; 
     Xaa at position 108 is Leu, Ala, Val, Arg, Trp, Gln or Gly; 
     Xaa at position 115 is Thr, His, Leu or Ala; 
     Xaa at position 120 is Gln, Gly, Arg, Lys or His 
     Xaa at position 123 is Glu, Arg, Phe or Thr 
     Xaa at position 144 is Phe, His, Arg, Pro, Leu, Gln or Glu; 
     Xaa at position 146 is Arg or Gln; 
     Xaa at position 147 is Arg or Gln; 
     Xaa at position 156 is His, Gly or Ser. 
     Xaa at position 159 is Ser, Arg, Thr, Tyr, Val or Gly; 
     Xaa at position 162 is Glu, Leu, Gly or Trp; 
     Xaa at position 163 is Val, Gly, Arg or Ala; 
     Xaa at position 169 is Arg, Ser, Leu, Arg or Cys; 
     Xaa at position 170 is His, Arg or Ser; 
     wherein optionally 1-11 amino acids from the N-terminus and 1-5 from the C-terminus can be deleted; and 
     wherein the N-terminus is joined to the C-terminus directly or through a linker capable of joining the N-terminus to the C-terminus and having new C- and N-termini at amino acids; 
     
       
         
               
               
               
             
           
               
                   
               
             
             
               
                 38-39 
                 62-63 
                 123-124 
               
               
                 39-40 
                 63-64 
                 124-125 
               
               
                 40-41 
                 64-65 
                 125-126 
               
               
                 41-42 
                 65-66 
                 126-127 
               
               
                 42-43 
                 66-67 
                 127-128 
               
               
                 43-44 
                 67-68 
                 128-129 
               
               
                 45-46 
                 68-69 
                 129-130 
               
               
                 48-49 
                 69-70 
                 130-131 
               
               
                 49-50 
                 70-71 
                 131-132 
               
               
                 52-53 
                 71-72 
                 132-133 
               
               
                 53-54 
                 91-92 
                 133-134 
               
               
                 54-55 
                 92-93 
                 134-135 
               
               
                 55-56 
                 93-94 
                 135-136 
               
               
                 56-57 
                 94-95 
                 136-137 
               
               
                 57-58 
                 95-96 
                 137-138 
               
               
                 58-59 
                 96-97 
                 138-139 
               
               
                 59-60 
                 97-98 
                 139-140 
               
               
                 60-61 
                 98-99 
                 140-141 
               
               
                 61-62 
                  99-100 
                 141-142 
               
               
                   
                   
                 or 142-143.     
               
               
                   
               
             
          
         
       
     
     The G-CSF receptor agonists of the present invention may contain amino acid substitutions, deletions and/or insertions and may also have amino acid deletions at either/or both the N- and C- termini. 
     The more preferred breakpoints at which new C-terminus and N-terminus can be made are; 38-39, 39-40, 40-41, 41-42, 48-49, 53-54, 54-55, 55-56, 56-57, 57-58, 58-59, 59-60, 60-61, 61-62, 62-63, 64-65, 65-66, 66-67, 67-68, 68-69, 69-70, 96-97, 125-126, 126-127, 127-128, 128-129, 129-130, 130-131, 131-132, 132-133, 133-134, 134-135, 135-136, 136-137, 137-138, 138-139, 139-140, 140-141 and 141-142. 
     The most preferred breakpoints at which new C-terminus and N-terminus can be made are; 38-39, 48-49, 96-97, 125-126, 132-133 and 141-142. 
     A preferred embodiment of the present invention the linker (L) joining the N-terminus to the C-terminus is a polypeptide selected from the group consisting of: 
     GlyGlyGlySer (SEQ ID NO:2); 
     GlyGlyGlySerGlyGlyGlySer (SEQ ID NO:61); 
     GlyGlyGlySerGlyGlyGlySerGlyGlyGlySer (SEQ ID NO:62); 
     SerGlyGlySerGlyGlySer (SEQ ID NO:63); 
     GluPheGlyAsnMet (SEQ ID NO:64); 
     GluPheGlyGlyAsnMet (SEQ ID NO:65); 
     GluPheGlyGlyAsnGlyGlyAsnMet (SEQ ID NO:66); and 
     GlyGlySerAspMetAlaGly (SEQ ID NO:67). 
     The present invention also includes a human G-CSF receptor agonist polypeptide, comprising a modified G-CSF amino acid sequence of the Formula: 
     
       
         
               
               
               
             
           
               
                 1                                   10 
                 (SEQ ID NO:1) 
                   
               
               
                 Xaa Xaa Xaa Gly Pro Ala Ser Ser Leu Pro Gln Ser Xaa 
               
               
                   
               
               
                                         20 
               
               
                 Leu Leu Xaa Xaa Xaa Glu Gln Val Xaa Lys Xaa Gln Gly Xaa Gly 
               
               
                   
               
               
                     30                                      40 
               
               
                 Ala Xaa Leu Gln Glu Xaa Leu Xaa Ala Thr Tyr Lys Leu Xaa Xaa 
               
               
                   
               
               
                                         50 
               
               
                 Xaa Glu Xaa Xaa Val Xaa Xaa Gly His Ser Xaa Gly Ile Pro Trp 
               
               
                   
               
               
                     60                                      70 
               
               
                 Ala Pro Leu Ser Ser Xaa Pro Ser Xaa Ala Leu Xaa Leu Ala Gly 
               
               
                   
               
               
                                         80 
               
               
                 Xaa Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu 
               
               
                   
               
               
                     90                                      100 
               
               
                 Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu 
               
               
                   
               
               
                                         110 
               
               
                 Xaa Thr Leu Gln Xaa Asp Val Ala Asp Phe Ala Xaa Thr Ile Trp 
               
               
                   
               
               
                     120                                     130 
               
               
                 Gln Gln Met Glu Xaa Xaa Gly Met Ala Pro Ala Leu Gln Pro Thr 
               
               
                   
               
               
                                         140 
               
               
                 Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Xaa Gln Xaa Xaa Ala 
               
               
                   
               
               
                     150                                     160 
               
               
                 Gly Gly Val Leu Val Ala Ser Xaa Leu Gln Xaa Phe Leu Xaa Xaa 
               
               
                   
               
               
                                         170 
               
               
                 Ser Tyr Arg Val Leu Xaa Xaa Leu Ala Gln Pro 
               
             
          
         
       
     
     wherein 
     Xaa at position 1 is Thr, Ser, Arg, Tyr or Gly; 
     Xaa at position 2 is Pro or Leu; 
     Xaa at position 3 is Leu, Arg, Tyr or Ser; 
     Xaa at position 13 is Phe, Ser, His, Thr or Pro; 
     Xaa at position 16 is Lys, Pro, Ser, Thr or His; 
     Xaa at position 17 is Cys, Ser, Gly, Ala, Ile, Tyr or Arg; 
     Xaa at position 18 is Leu, Thr, Pro, His, Ile or Cys; 
     Xaa at position 22 is Arg, Tyr, Ser, Thr or Ala; 
     Xaa at position 24 is Ile, Pro, Tyr or Leu; 
     Xaa at position 27 is Asp, or Gly; 
     Xaa at position 30 is Ala, Ile, Leu or Gly; 
     Xaa at position 34 is Lys or Ser; 
     Xaa at position 36 is Cys or Ser; 
     Xaa at position 42 is Cys or Ser; 
     Xaa at position 43 is His, Thr, Gly, Val, Lys, Trp, Ala, Arg, Cys, or Leu; 
     Xaa at position 44 is Pro, Gly, Arg, Asp, Val, Ala, His, Trp, Gln, or Thr; 
     Xaa at position 46 is Glu, Arg, Phe, Arg, Ile or Ala; 
     Xaa at position 47 is Leu or Thr; 
     Xaa at position 49 is Leu, Phe, Arg or Ser; 
     Xaa at position 50 is Leu, Ile, His, Pro or Tyr; 
     Xaa at position 54 is Leu or His; 
     Xaa at position 64 is Cys or Ser; 
     Xaa at position 67 is Gln, Lys, Leu or Cys; 
     Xaa at position 70 is Gln, Pro, Leu, Arg or Ser; 
     Xaa at position 74 is Cys or Ser; 
     Xaa at position 104 is Asp, Gly or Val; 
     Xaa at position 108 is Leu, Ala, Val, Arg, Trp, Gln or Gly; 
     Xaa at position 115 is Thr, His, Leu or Ala; 
     Xaa at position 120 is Gln, Gly, Arg, Lys or His 
     Xaa at position 123 is Glu, Arg, Phe or Thr 
     Xaa at position 144 is Phe, His, Arg, Pro, Leu, Gln or Glu; 
     Xaa at position 146 is Arg or Gln; 
     Xaa at position 147 is Arg or Gln; 
     Xaa at position 156 is His, Gly or Ser; 
     Xaa at position 159 is Ser, Arg, Thr, Tyr, Val or Gly; 
     Xaa at position 162 is Glu, Leu, Gly or Trp; 
     Xaa at position 163 is Val, Gly, Arg or Ala; 
     Xaa at position 169 is Arg, Ser, Leu, Arg or Cys; 
     Xaa at position 170 is His, Arg or Ser; 
     wherein optionally 1-11 amino acids from the N-terminus and 1-5 from the C-terminus can be deleted; 
     wherein the N-terminus is joined to the C-terminus directly or through a linker capable of joining the N-terminus to the C-terminus and having new C- and N-terminus at amino acids;. 
     2-3 
     10-11 
     12-13 
     18-19 
     122-123 
     158-159 
     169-170. 
     The present invention also encompasses recombinant human G-CSF receptor agonists co-administered or sequentially with one or more additional colony stimulating factors (CSF) including, cytokines, lymphokines, interleukins, hematopoietic growth factors which include but are not limited to GM-CSF, c-mpl ligand (also known as TPO or MGDF), M-CSF, erythropoietin (EPO), IL-1, IL-4, IL-2, IL-3, IL-5, IL 6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, LIF, flt3/flk2 ligand, human growth hormone, B-cell growth factor, B-cell differentiation factor, eosinophil differentiation factor and stem cell factor (SCF) also known as steel factor or c-kit ligand (herein collectively referred to as “colony stimulating factors” or “CSF”). These co-administered mixtures may be characterized by having the usual activity of both of the peptides or the mixture may be further characterized by having a biological or physiological activity greater than simply the additive function of the presence of the G-CSF receptor agonists or the second colony stimulating factor alone. The co-administration may also provide an enhanced effect on the activity or an activity different from that expected by the presence of the G-CSF ligand or the second colony stimulating factor. The co-administration may also have an improved activity profile which may include reduction of undesirable biological activities associated with native human G-CSF. In addition to the list above, IL-3 variants taught in WO 94/12639 and WO 94/12638 can be co-administered with the polypeptides of the present invention. 
     In addition, it is envisioned that in vitro uses would include the ability to stimulate bone marrow and blood cell activation and growth before the expanded cells are infused into patients. 
    
    
     BRIEF DESCRIPTION OF THE FIGURES 
     FIG. 1 schematically illustrates the sequence rearrangement of a protein. The N-terminus (N) and the C-terminus (C) of the native protein are joined through a linker, or joined directly. The protein is opened at a breakpoint creating a new N-terminus (new N) and a new C-terminus (new-C) resulting in a protein with a new linear amino acid sequence. A rearranged molecule may be synthesized de novo as linear molecule and not go through the steps of joining the original N-terminus and the C-terminus and opening of the protein at the breakpoint. 
     FIG. 2 shows a schematic of Method I, for creating new proteins in which the original N-terminus and C-terminus of the native protein are joined with a linker and different N-terminus and C-terminus of the protein are created. In the example shown the sequence rearrangement results in a new gene encoding a protein with a new N-terminus created at amino acid 97 of the original protein, the original C-terminus (a.a. 174) joined to the amino acid 11 (a.a. 1-10 are deleted) through a linker region and a new C-terminus created at amino acid 96 of the original sequence. 
     FIG. 3 shows a schematic of Method II, for creating new proteins in which the original N-terminus and C-terminus of the native protein are joined without a linker and different N-terminus and C-terminus of the protein are created. In the example shown the sequence rearrangement results in a new gene encoding a protein with a new N-terminus created at amino acid 97 of the original protein, the original C-terminus (a.a. 174) joined to the original N-terminus and a new C-terminus created at amino acid 96 of the original sequence. 
     FIG. 4 shows a schematic of Method III, for creating new proteins in which the original N-terminus and C-terminus of the native protein are joined with a linker and different N-terminus and C-terminus of the protein are created. In the example shown the sequence rearrangement results in a new gene encoding a protein with a new N-terminus created at amino acid 97 of the original protein, the original C-terminus (a.a. 174) joined to amino acid 1 through a linker region and a new C-terminus created at amino acid 96 of the original sequence. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Receptor agonists of the present invention may be useful in the treatment of diseases characterized by decreased levels of granulocytes of the hematopoietic system. 
     A G-CSF receptor agonist may be useful in the treatment or prevention of neutropenia. Many drugs may cause bone marrow suppression or hematopoietic deficiencies. Examples of such drugs are AZT, DDI, alkylating agents and anti-metabolites used in chemotherapy, antibiotics such as chloramphenicol, penicillin, gancyclovir, daunomycin and sulfa drugs, phenothiazones, tranquilizers such as meprobamate, analgesics such as aminopyrine and dipyrone, anti-convulsants such as phenytoin or carbamazepine, antithyroids such as propylthiouracil and methimazole and diuretics. G-CSF receptor agonists may be useful in preventing or treating the bone marrow suppression or hematopoietic deficiencies which often occur in patients treated with these drugs. 
     Hematopoietic deficiencies may also occur as a result of viral, microbial or parasitic infections and as a result of treatment for renal disease or renal failure, e.g., dialysis. The present peptide may be useful in treating such hematopoietic deficiency. 
     Another aspect of the present invention provides plasmid DNA vectors for use in the method of expression of these novel G-CSF receptor agonists. These vectors contain the novel DNA sequences described above which code for the novel polypeptides of the invention. Appropriate vectors which can transform host cells capable of expressing the G-CSF receptor agonists include expression vectors comprising nucleotide sequences coding for the G-CSF receptor agonists joined to transcriptional and translational regulatory sequences which are selected according to the host cells used. Vectors incorporating modified sequences as described above are included in the present invention and are useful in the production of the modified G-CSF receptor agonist polypeptides. The vector employed in the method also contains selected regulatory sequences in operative association with the DNA coding sequences of the invention and capable of directing the replication and expression thereof in selected host cells. 
     As another aspect of the present invention, there is provided a novel method for producing the novel family of human G-CSF receptor agonists. The method of the present invention involves culturing suitable cells or cell line, which has been transformed with a vector containing a DNA sequence coding for expression of the novel G-CSF receptor agonist polypeptide. Suitable cells or cell lines may include various strains of bacteria such as  E. coli,  yeast, mammalian cells, or insect cells may be utilized as host cells in the method of the present invention. 
     Other aspects of the present invention are methods and therapeutic compositions for treating the conditions referred to above. Such compositions comprise a therapeutically effective amount of one or more of the G-CSF receptor agonists of the present invention in a mixture with a pharmaceutically acceptable carrier. This composition can be administered either parenterally, intravenously or subcutaneously. When administered, the therapeutic composition for use in this invention is preferably in the form of a pyrogen-free, parenterally acceptable aqueous solution. The preparation of such a parenterally acceptable protein solution, having due regard to pH, isotonicity, stability and the like, is within the skill of the art. 
     The dosage regimen involved in a method for treating the above-described conditions will be determined by the attending physician considering various factors which modify the action of drugs, e.g. the condition, body weight, sex and diet of the patient, the severity of any infection, time of administration and other clinical factors. Generally, a daily regimen may be in the range of 0.5-150 μg/kg of non-glycosylated G-CSF receptor agonists protein per kilogram of body weight. Dosages would be adjusted relative to the activity of a given receptor agonist and it would not be unreasonable to note that dosage regimens may include doses as low as 0.1 microgram and as high as 1 milligram per kilogram of body weight per day. In addition, there may exist specific circumstances where dosages of G-CSF receptor agonist would be adjusted higher or lower than the range of 0.5-150 micrograms per kilogram of body weight. These include co-administration with other CSF or growth factors; co-administration with chemotherapeutic drugs and/or radiation; the use of glycosylated G-CSF receptor agonists; and various patient-related issues mentioned earlier in this section. As indicated above, the therapeutic method and compositions may also include co-administration with other human factors. A non-exclusive list of other appropriate hematopoietins, CSFs and interleukins for simultaneous or serial co-administration with the polypeptides of the present invention includes GM-CSF, c-mpl ligand (also known as TPO or MGDF), M-CSF, erythropoietin (EPO), IL-1, IL-4, IL-2, IL-3, IL-5, IL 6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, LIF, flt3/flk2 ligand, human growth hormone, B-cell growth factor, B-cell differentiation factor, eosinophil differentiation factor and stem cell factor (SCF) also known as steel factor or c-kit ligand (herein collectively referred to as “colony stimulating factors”), or combinations thereof. In addition to the list above, IL-3 variants taught in WO 94/12639 and WO 94/12638 can be co-administered with the polypeptides of the present invention. 
     The G-CSF receptor agonists of the present invention may be useful in the mobilization of hematopoietic progenitors and stem cells in peripheral blood. Peripheral blood derived progenitors have been shown to be effective in reconstituting patients in the setting of autologous marrow transplantation. Hematopoietic growth factors, including G-CSF and GM-CSF, have been shown to enhance the number of circulating progenitors and stem cells in the peripheral blood. This has simplified the procedure for peripheral stem cell collection and dramatically decreased the cost of the procedure by decreasing the number of pheresis required. The G-CSF receptor agonist of the present invention may be useful in mobilization of stem cells and further enhance the efficacy of peripheral stem cell transplantation. 
     The G-CSF receptor agonists of the present invention may also be useful in the ex vivo expansion of hematopoietic progenitors. Colony stimulating factors (CSFs), such as G-CSF, have been administered alone, co-administered with other CSFs, or in combination with bone marrow transplants subsequent to high dose chemotherapy to treat the neutropenia and which is often the result of such treatment. However the period of severe neutropenia may not be totally eliminated. The myeloid lineage, which is comprised of monocytes (macrophages), granulocytes (including neutrophils) and megakaryocytes, is critical in preventing infections and bleeding which can be life-threatening. Neutropenia may also be the result of disease, genetic disorders, drugs, toxins, radiation and many therapeutic treatments such as conventional oncology therapy. 
     Bone marrow transplants have been used to treat this patient population. However, several problems are associated with the use of bone marrow to reconstitute a compromised hematopoietic system including: 1) the number of stem cells in bone marrow or other tissues, such as spleen or peripheral blood, is limited, 2) Graft Versus Host Disease, 3) graft rejection and 4) possible contamination with tumor cells. Stem cells and progenitor cells make up a very small percentage of the nucleated cells in the bone marrow, spleen and peripheral blood. It is clear that a dose response exists such that a greater number of multipotential hematopoietic progenitors will enhance hematopoietic recovery. Therefore, the in vitro expansion of stem cells should enhance hematopoietic recovery and patient survival. Bone marrow from an allogeneic donor has been used to provide bone marrow for transplant. However, Graft Versus Host Disease and graft rejection limit bone marrow transplantation even in recipients with HLA-matched sibling donors. An alternative to allogeneic bone marrow transplants is autologous bone marrow transplants. In autologous bone marrow transplants, some of the patient&#39;s own marrow is harvested prior to myeloablative therapy, e.g. high dose chemotherapy, and is transplanted back into the patient afterwards. Autologous transplants eliminate the risk of Graft Versus Host Disease and graft rejection. However, autologous bone marrow transplants still present problems in terms of the limited number of stems cells in the marrow and possible contamination with tumor cells. The limited number of multipotential hematopoietic progenitors may be overcome by ex-vivo expansion of the multipotential hematopoietic progenitors. In addition, stem cells can be specifically isolated based on the presence of specific surface antigens such as CD34+ in order to decrease tumor cell contamination of the marrow graft. 
     The following patents contain further details on separating stem cells, CD34+ cells, culturing the cells with hematopoietic factors, the use of the cells for the treatment of patients with hematopoietic disorders and the use of hematopoietic factors for cell expansion and gene therapy. 
     U.S. Pat. No. 5,061,620 relates to compositions comprising human hematopoietic stem cells provided by separating the stem cells from dedicated cells. 
     U.S. Pat. No. 5,199,942 describes a method for autologous hematopoietic cell transplantation comprising: (1) obtaining hematopoietic progenitor cells from a patient; (2) ex-vivo expansion of cells with a growth factor selected from the group consisting of IL-3, flt3 ligand, c-kit ligand, GM CSF, IL-1, GM-CSF/IL-3 fusion protein and combinations thereof; (3) administering cellular preparation to a patient. 
     U.S. Pat. No. 5,240,856 relates to a cell separator that includes an apparatus for automatically controlling the cell separation process. 
     WO 91/16116 describes devices and methods for selectively isolating and separating target cells from a mixture of cells. 
     WO 91/18972 describes methods for in vitro culturing of bone marrow, by incubating suspension of bone marrow cells, using a hollow fiber bioreactor. 
     WO 92/18615 relates to a process for maintaining and expanding bone marrow cells, in a culture medium containing specific mixtures of cytokines, for use in transplants. 
     WO 93/08268 describes a method for selectively expanding stem cells, comprising the steps of (a) separating CD34+ stem cells from other cells and (b) incubating the separated cells in a selective medium, such that the stem cells are selectively expanded. 
     WO 93/18136 describes a process for in vitro support of mammalian cells derived from peripheral blood. 
     WO 93/18648 relates to a composition comprising human neutrophil precursor cells with a high content of myeloblasts and promyelocytes for treating genetic or acquired neutropenia. 
     WO 94/08039 describes a method of enrichment for human hematopoietic stem cells by selection for cells which express c-kit protein. 
     WO 94/11493 describes a stem cell population that are CD34+ and small in size, which are isolated using a counterflow elutriation method. 
     WO 94/27698 relates to a method combining immunoaffinity separation and continuous flow centrifugal separation for the selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. 
     WO 94/25848 describes a cell separation apparatus for collection and manipulation of target cells. 
     The long term culturing of highly enriched CD34+ precursors of hematopoietic progenitor cells from human bone marrow in cultures containing IL-1α, IL-3, IL-6 or GM-CSF is discussed in Brandt et al ( J. Clin. Invest.  86:932-941, 1990). 
     One aspect of the present invention provides a method for selective ex-vivo expansion of stem cells. The term “stem cell” refers to the multipotential hematopoietic cells as well as early myeloid progenitor and precursors cells which can be isolated from bone marrow, spleen or peripheral blood. The term “expansion” refers to the proliferation and differentiation of the cells. The present invention provides a method for selective ex-vivo expansion of stem cells, comprising the steps of; (a) separating stem cells from other cells, (b) culturing the separated stem cells with a selective medium which contains a G-CSF receptor agonist and optionally a second colony stimulating factor, and (c) harvesting the cultured stems cells. Stem cells, as well as committed progenitor cells destined to become neutrophils, erythrocytes, platelets, etc., may be distinguished from most other cells by the presence or absence of particular progenitor marker antigens, such as CD34, that are present on the surface of these cells and/or by morphological characteristics. The phenotype for a highly enriched human stem cell fraction is reported as CD34+, Thy-1+ and lin-, but it is to be understood that the present invention is not limited to the expansion of this stem cell population. The CD34+ enriched human stem cell fraction can be separated by a number of reported methods, including affinity columns or beads, magnetic beads or flow cytometry using antibodies directed to surface antigens such as the CD34+. Further, physical separation methods such as counterflow elutriation may be used to enrich hematopoietic progenitors. The CD34+ progenitors are heterogeneous, and may be divided into several sub-populations characterized by the presence or absence of co-expression of different lineage associated cell surface associated molecules. The most immature progenitor cells do not express any known lineage associated markers, such as HLA-DR or CD38, but they may express CD90(thy-1). Other surface antigens such as CD33, CD38, CD41, CD71, HLA-DR or c-kit can also be used to selectively isolate hematopoietic progenitors. The separated cells can be incubated in selected medium in a culture flask, sterile bag or in hollow fibers. Various colony stimulating factors may be utilized in order to selectively expand cells. Representative factors that have been utilized for ex-vivo expansion of bone marrow include, c-kit ligand, IL-3, G-CSF, GM-CSF, IL-1, IL-6, IL-11, flt-3 ligand or combinations thereof. The proliferation of the stem cells can be monitored by enumerating the number of stem cells and other cells, by standard techniques (e.g. hemacytometer, CFU, LTCIC) or by flow cytometry prior and subsequent to incubation. 
     Several methods for ex-vivo expansion of stem cells have been reported utilizing a number of selection methods and expansion using various colony stimulating factors including c-kit ligand (Brandt et al.,  Blood  83:1507-1514, 1994; McKenna et al.,  Blood  86:3413-3420, 1995), IL-3 (Brandt et al.,  Blood  83:1507-1514, 1994; Sato et al.,  Blood  82:3600-3609, 1993), G-CSF (Sato et al.,  Blood  82:3600-3609, 1993), GM-CSF (Sato et al.,  Blood  82:3600-3609, 1993), IL-1 (Muench et al.,  Blood  81:3463-3473, 1993), IL-6 (Sato et al.,  Blood  82:3600-3609, 1993), IL-11 (Lemoli et al.,  Exp. Hem.  21:1668-1672, 1993; Sato et al.,  Blood  82:3600-3609, 1993), flt-3 ligand (McKenna et al.,  Blood  86:3413 3420, 1995) and/or combinations thereof (Brandt et al.,  Blood  83:1507 1514, 1994; Haylock et al.,  Blood  80:1405-1412, 1992, Koller et al.,  Biotechnology  11:358-363, 1993; Lemoli et al.,  Exp. Hem.  21:1668-1672, 1993), McKenna et al.,  Blood  86:3413-3420, 1995; Muench et al.,  Blood  81:3463-3473, 1993; Patchen et al.,  Biotherapy  7:13-26, 1994; Sato et al.,  Blood  82:3600-3609, 1993; Smith et al.,  Exp. Hem.  21:870-877, 1993; Steen et al.,  Stem Cells  12:214-224, 1994; Tsujino et al.,  Exp. Hem.  21:1379-1386, 1993). Among the individual colony stimulating factors, hIL-3 has been shown to be one of the most potent in expanding peripheral blood CD34+ cells (Sato et al.,  Blood  82:3600-3609, 1993; Kobayashi et al.,  Blood  73:1836-1841, 1989). However, no single factor has been shown to be as effective as the combination of multiple factors. The present invention provides methods for ex vivo expansion that utilize novel G-CSF receptor agonists. 
     Another aspect of the invention provides methods of sustaining and/or expanding hematopoietic precursor cells which includes inoculating the cells into a culture vessel which contains a culture medium that has been conditioned by exposure to a stromal cell line such as HS-5 (WO 96/02662, Roecklein and Torok-Strob,  Blood  85:997-1105, 1995) that has been supplemented with a G-CSF receptor agonist of the present invention. 
     Another projected clinical use of growth factors has been in the in vitro activation of hematopoietic progenitors and stem cells for gene therapy. Due to the long life-span of hematopoietic progenitor cells and the distribution of their daughter cells throughout the entire body, hematopoietic progenitor cells are good candidates for ex vivo gene transfection. In order to have the gene of interest incorporated into the genome of the hematopoietic progenitor or stem cell one needs to stimulate cell division and DNA replication. Hematopoietic stem cells cycle at a very low frequency which means that growth factors may be useful to promote gene transduction and thereby enhance the clinical prospects for gene therapy. Potential applications of gene therapy (review Crystal,  Science  270:404-410, 1995) include; 1) the treatment of many congenital metabolic disorders and immunodeficiencies (Kay and Woo,  Trends Genet.  10:253-257, 1994), 2) neurological disorders (Friedmann,  Trends Genet.  10:210-214, 1994), 3) cancer (Culver and Blaese,  Trends Genet.  10:174-178, 1994) and 4) infectious diseases (Gilboa and Smith,  Trends Genet.  10:139-144, 1994). 
     There are a variety of methods, known to those with skill in the art, for introducing genetic material into a host cell. A number of vectors, both viral and non-viral have been developed for transferring therapeutic genes into primary cells. Viral based vectors include; 1) replication deficient recombinant retrovirus (Boris-Lawrie and Temin,  Curr. Opin. Genet. Dev.  3:102-109, 1993; Boris-Lawrie and Temin,  Annal. New York Acad. Sci.  716:59-71, 1994; Miller,  Current Top. Microbiol. Immunol.  158:1-24, 1992) and replication-deficient recombinant adenovirus (Berkner,  BioTechniques  6:616-629, 1988; Berkner,  Current Top. Microbiol. Immunol.  158:39-66, 1992; Brody and Crystal,  Annal. New York Acad. Sci.  716:90-103, 1994). Non-viral based vectors include protein/DNA complexes (Cristiano et al.,  PNAS USA.  90:2122-2126, 1993; Curiel et al.,  PNAS USA  88:8850-8854, 1991; Curiel,  Annal. New York Acad. Sci.  716:36-58, 1994), electroporation and liposome mediated delivery such as cationic liposomes (Farhood et al.,  Annal. New York Acad. Sci.  716:23-35, 1994). 
     The present invention provides an improvement to the existing methods of expanding hematopoietic cells, into which new genetic material has been introduced, in that it provides methods utilizing G-CSF receptor agonists that may have improved biological activity and/or physical properties. 
     Determination of the Linker 
     The length of the amino acid sequence of the linker can be selected empirically or with guidance from structural information, or by using a combination of the two approaches. 
     When no structural information is available, a small series of linkers can be prepared for testing using a design whose length is varied in order to span a range from 0 to 50 Å and whose sequence is chosen in order to be consistent with surface exposure (hydrophilicity, Hopp &amp; Woods,  Mol. Immunol.  20: 483-489, 1983; Kyte &amp; Doolittle,  J. Mol. Biol.  157:105-132, 1982; solvent exposed surface area, Lee &amp; Richards,  J. Mol. Biol.  55:379-400, 1971) and the ability to adopt the necessary conformation without deranging the configuration of the c-mpl receptor agonist (conformationally flexible; Karplus &amp; Schulz,  Naturwissenschaften  72:212-213, (1985). Assuming an average of translation of 2.0 to 3.8 Å per residue, this would mean the length to test would be between 0 to 30 residues, with 0 to 15 residues being the preferred range. Exemplary of such an empirical series would be to construct linkers using a cassette sequence such as Gly-Gly-Gly-Ser (SEQ ID NO:2) repeated n times, where n is 1, 2, 3 or 4. Those skilled in the art will recognize that there are many such sequences that vary in length or composition that can serve as linkers with the primary consideration being that they be neither excessively long nor short (cf., Sandhu,  Critical Rev. Biotech.  12: 437-462, 1992); if they are too long, entropy effects will likely destabilize the three-dimensional fold, and may also make folding kinetically impractical, and if they are too short, they will likely destabilize the molecule because of torsional or steric strain. 
     Those skilled in the analysis of protein structural information will recognize that using the distance between the chain ends, defined as the distance between the c-alpha carbons, can be used to define the length of the sequence to be used, or at least to limit the number of possibilities that must be tested in an empirical selection of linkers. They will also recognize that it is sometimes the case that the positions of the ends of the polypeptide chain are ill-defined in structural models derived from x-ray diffraction or nuclear magnetic resonance spectroscopy data, and that when true, this situation will therefore need to be taken into account in order to properly estimate the length of the linker required. From those residues whose positions are well defined are selected two residues that are close in sequence to the chain ends, and the distance between their c-alpha carbons is used to calculate an approximate length for a linker between them. Using the calculated length as a guide, linkers with a range of number of residues (calculated using 2 to 3.8 Å per residue) are then selected. These linkers may be composed of the original sequence, shortened or lengthened as necessary, and when lengthened the additional residues may be chosen to be flexible and hydrophilic as described above; or optionally the original sequence may be substituted for using a series of linkers, one example being the Gly-Gly-Gly-Ser (SEQ ID NO:2) cassette approach mentioned above; or optionally a combination of the original sequence and new sequence having the appropriate total length may be used. 
     Determination of the Amino and Carboxyl Termini of G-CSF Receptor Agonists 
     Sequences of G-CSF receptor agonists capable of folding to biologically active states can be prepared by appropriate selection of the beginning (amino terminus) and ending (carboxyl terminus) positions from within the original polypeptide chain while using the linker sequence as described above. Amino and carboxyl termini are selected from within a common stretch of sequence, referred to as a breakpoint region, using the guidelines described below. A novel amino acid sequence is thus generated by selecting amino and carboxyl termini from within the same breakpoint region. In many cases the selection of the new termini will be such that the original position of the carboxyl terminus immediately preceded that of the amino terminus. However, those skilled in the art will recognize that selections of termini anywhere within the region may function, and that these will effectively lead to either deletions or additions to the amino or carboxyl portions of the new sequence. 
     It is a central tenet of molecular biology that the primary amino acid sequence of a protein dictates folding to the three-dimensional structure necessary for expression of its biological function. Methods are known to those skilled in the art to obtain and interpret three-dimensional structural information using x-ray diffraction of single protein crystals or nuclear magnetic resonance spectroscopy of protein solutions. Examples of structural information that are relevant to the identification of breakpoint regions include the location and type of protein secondary structure (alpha and 3-10 helices, parallel and anti-parallel beta sheets, chain reversals and turns, and loops; Kabsch &amp; Sander,  Biopolymers  22: 2577-2637, 1983; the degree of solvent exposure of amino acid residues, the extent and type of interactions of residues with one another (Chothia,  Ann. Rev. Biochem.  53:537-572; 1984) and the static and dynamic distribution of conformations along the polypeptide chain (Alber &amp; Mathews,  Methods Enzymol.  154: 511-533, 1987). In some cases additional information is known about solvent exposure of residues; one example is a site of post-translational attachment of carbohydrate which is necessarily on the surface of the protein. When experimental structural information is not available, or is not feasible to obtain, methods are also available to analyze the primary amino acid sequence in order to make predictions of protein tertiary and secondary structure, solvent accessibility and the occurrence of turns and loops. Biochemical methods are also sometimes applicable for empirically determining surface exposure when direct structural methods are not feasible; for example, using the identification of sites of chain scission following limited proteolysis in order to infer surface exposure (Gentile &amp; Salvatore,  Eur. J. Biochem.  218:603-621, 1993) Thus using either the experimentally derived structural information or predictive methods (e.g., Srinivisan &amp; Rose  Proteins: Struct., Funct. &amp; Genetics,  22: 81-99, 1995) the parental amino acid sequence is inspected to classify regions according to whether or not they are integral to the maintenance of secondary and tertiary structure. The occurrence of sequences within regions that are known to be involved in periodic secondary structure (alpha and 3-10 helices, parallel and anti-parallel beta sheets) are regions that should be avoided. Similarly, regions of amino acid sequence that are observed or predicted to have a low degree of solvent exposure are more likely to be part of the so-called hydrophobic core of the protein and should also be avoided for selection of amino and carboxyl termini. In contrast, those regions that are known or predicted to be in surface turns or loops, and especially those regions that are known not to be required for biological activity, are the preferred sites for location of the extremes of the polypeptide chain. Continuous stretches of amino acid sequence that are preferred based on the above criteria are referred to as a breakpoint region. 
     
       
         
               
             
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 OLIGONUCLEOTIDES 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 L-11start.seq 
                 GCTCTGAGAG CCGCCAGAGC CGCCAGAGGG 
               
               
                   
                 CTGCGCAAGG TGGCGTAGAA CGCG 
               
               
                   
                 (SEQ ID NO: 3) 
               
               
                 L-11stop.seq 
                 CAGCCCTCTG GCGGCTCTGG CGGCTCTCAG 
               
               
                   
                 AGCTTCCTGC TCAAGTCTTT AGAG 
               
               
                   
                 (SEQ ID NO: 4) 
               
               
                 BlstartP.seq 
                 GGGCTGCGCA AGGTGGCG (SEQ ID NO: 5) 
               
               
                 blstopP.seq 
                 ACACCATTGG GCCCTGCCAG C (SEQ ID NO: 6) 
               
               
                 39start.seq 
                 GATCGACCAT GGCTTACAAG CTGTGCCACC CC 
               
               
                   
                 (SEQ ID NO: 7) 
               
               
                 38stop.Seq 
                 CGATCGAAGC TTATTAGGTG GCACACAGCT 
               
               
                   
                 TCTCCT (SEQ ID NO: 8) 
               
               
                 97start.seq 
                 GATCGACCAT GGCTCCCGAG TTGGGTCCCA CC 
               
               
                   
                 (SEQ ID NO: 9) 
               
               
                 96stop.Seq 
                 CGATCGAAGC TTATTAGGAT ATCCCTTCCA 
               
               
                   
                 GGGCCT (SEQ ID NO: 10) 
               
               
                 126start.seq 
                 GATCGACCAT GGCTATGGCC CCTGCCCTGC AG 
               
               
                   
                 (SEQ ID NO: 11) 
               
               
                 125stop.Seq 
                 CGATCGAAGC TTATTATCCC AGTTCTTCCA 
               
               
                   
                 TCTGCT (SEQ ID NO: 12) 
               
               
                 133start.seq 
                 GATCGACCAT GGCTACCCAG GGTGCCATGC CG 
               
               
                   
                 (SEQ ID NO: 13) 
               
               
                 132stop.seq 
                 CGATCGAAGC TTATTAGGGC TGCAGGGCAG 
               
               
                   
                 GGGCCA (SEQ ID NO: 14) 
               
               
                 142start.seq 
                 GATCGACCAT GGCTTCTGCT TTCCAGCGCC GG 
               
               
                   
                 (SEQ ID NO: 15) 
               
               
                 141stop.Seq 
                 CGATCGAAGC TTATTAGGCG AAGGCCGGCA 
               
               
                   
                 TGGCAC (SEQ ID NO: 16) 
               
               
                 96for.Seq 
                 ATATCCATGG CTCCGGAACT GGGTCCAACT CTG 
               
               
                   
                 (SEQ ID NO: 17) 
               
               
                 96rev.Seq 
                 ACCTCCAGGA AGCTCTGCAG ATGG 
               
               
                   
                 (SEQ ID NO: 18) 
               
               
                 125for.seq 
                 TATATCCATG GCTATGGCTC CAGCTCTGCA 
               
               
                   
                 ACCAACTCAA GGTGCAATGC CAGCATTTGC 
               
               
                   
                 ATCTG (SEQ ID NO: 19) 
               
               
                 125rev.seq 
                 GATGGCTAGC AACCAGAACA CCACCTGCAC 
               
               
                   
                 GACGTTGAAA AGCAGATGCA AATGCTGGCA TTG 
               
               
                   
                 (SEQ ID NO: 20) 
               
               
                 132for.seq 
                 TATATCCATG GCTACTCAAG GTGCTATGCC 
               
               
                   
                 AGCTTTTGCT TCTGCTTTTC AACGTCG 
               
               
                   
                 (SEQ ID NO: 21) 
               
               
                 132rev.seq 
                 GCAGATGGCT AGCAACCAGA ACACCACCTG 
               
               
                   
                 CACGACGTTG AAAAGCAGAA GCAAAAGC 
               
               
                   
                 (SEQ ID NO: 22) 
               
               
                 141for.seq 
                 CATGGCTTCT GCTTTTCAAC GTCGTGCAGG 
               
               
                   
                 TGGTGTTCTG GTTG (SEQ ID NO: 23) 
               
               
                 141rev.seq 
                 CTAGCAACCA GAACACCACC TGCACGACGT 
               
               
                   
                 TGAAAAGCAG AAGC (SEQ ID NO: 24) 
               
               
                 49start.seq 
                 GATCGACCAT GGCTCTGCTC GGACACTCTC TG 
               
               
                   
                 (SEQ ID NO: 68) 
               
               
                 48stop.seq 
                 CGATCGAAGC TTATTACACC AGCTCCTCGG 
               
               
                   
                 GGTGGC (SEQ ID NO: 69) 
               
               
                 77start.seq 
                 GATCGACCAT GGCTCAACTC CATAGCGGCC TT 
               
               
                   
                 (SEQ ID NO: 70) 
               
               
                 76stop.seq 
                 CGATCGAAGC TTATTAGCTC AAGCAGCCTG 
               
               
                   
                 CCAGCT (SEQ ID NO: 71) 
               
               
                 82start.seq 
                 GATCGACCAT GGCTCTTTTC CTCTACCAGG GG 
               
               
                   
                 (SEQ ID NO: 72) 
               
               
                 81stop.seq 
                 CGATCGAAGC TTATTAGCCG CTATGGAGTT 
               
               
                   
                 GGCTCA (SEQ ID NO: 73) 
               
               
                 84start.seq 
                 GATCGACCAT GGCTCTCTAC CAGGGGCTCC TG 
               
               
                   
                 (SEQ ID NO: 74) 
               
               
                 83stop.seq 
                 CGATCGAAGC TTATTAGAAA AGGCCGCTAT 
               
               
                   
                 GGAGTT (SEQ ID NO: 75) 
               
               
                 91start.seq 
                 GATCGACCAT GGCTGCCCTG GAAGGGATAT CC 
               
               
                   
                 (SEQ ID NO: 76) 
               
               
                 90stop.seq 
                 CGATCGAAGC TTATTACTGC AGGAGCCCCT 
               
               
                   
                 GGTAGA (SEQ ID NO: 77) 
               
               
                 112start.seq 
                 GATCGACCAT GGCTGACTTT GCCACCACCA TC 
               
               
                   
                 (SEQ ID NO: 78) 
               
               
                 111stop.seq 
                 CGATCGAAGC TTATTAGGCG ACGTCCAGCT 
               
               
                   
                 GCAGTG (SEQ ID NO: 79) 
               
               
                 117start.seq 
                 GATCGACCAT GGCTATCTGG CAGCAGATGG AA 
               
               
                   
                 (SEQ ID NO: 80) 
               
               
                 116stop.seq 
                 CGATCGAAGC TTATTAGGTG GTGGCAAAGT 
               
               
                   
                 CGGCGA (SEQ ID NO: 81) 
               
               
                 119start.seq 
                 GATCGACCAT GGCTCAGCAG ATGGAAGAAC TG 
               
               
                   
                 (SEQ ID NO: 82) 
               
               
                 118stop.seq 
                 CGATCGAAGC TTATTACCAG ATGGTGGTGG 
               
               
                   
                 CAAAGT (SEQ ID NO: 83) 
               
               
                 Z4849at.for 
                 CATGGCTTTG TTAGGACATT CTTTAGGTAT 
               
               
                   
                 TCCATGGGCT CCTCTGAGCT (SEQ ID NO: 84) 
               
               
                 Z4849at.rev 
                 CAGAGGAGCC CATGGAATAC CTAAAGAATG 
               
               
                   
                 TCCTAACAAA GC (SEQ ID NO: 85) 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
             
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                 DNA sequences 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 pMON3485.Seq 
               
             
          
           
               
                   1 ATGGCTTACA AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC 
                 (SEQ ID NO:25) 
               
               
                   
               
               
                  51 TCTGGGCATC CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC 
               
               
                   
               
               
                 101 AGCTGGCAGG CTGCTTGAGC CAACTCCATA GCGGCCTTTT CCTCTACCAG 
               
               
                   
               
               
                 151 GGGCTCCTGC AGGCCCTGGA AGGGATATCC CCCGAGTTGG GTCCCACCTT 
               
               
                   
               
               
                 201 GGACACACTG CAGCTGGACG TCGCCGACTT TGCCACCACC ATCTGGCAGC 
               
               
                   
               
               
                 251 AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC 
               
               
                   
               
               
                 301 ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC CGGGCAGGAG GGGTCCTGGT 
               
               
                   
               
               
                 351 TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT GTCGTACCGC GTTCTACGCC 
               
               
                   
               
               
                 401 ACCTTGCGCA GCCCTCTGGC GGCTCTGGCG GCTCTCAGAG CTTCCTGCTC 
               
               
                   
               
               
                 451 AAGTCTTTAG AGCAAGTGAG GAAGATCCAG GGCGATGGCG CAGCGCTCCA 
               
               
                   
               
               
                 501 GGAGAAGCTG TGTGCCACCT AATAA 
               
               
                   
               
               
                 pMON3486.Seq 
               
               
                   
               
               
                   1 ATGGCTCCCG AGTTGGGTCC CACCTTGGAC ACACTGCAGC TGGACGTCGC 
                 (SEQ ID NO:26) 
               
               
                   
               
               
                  51 CGACTTTGCC ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC 
               
               
                   
               
               
                 101 CTGCCCTGCA GCCCACCCAG GGTGCCATGC CGGCCTTCGC CTCTGCTTTC 
               
               
                   
               
               
                 151 CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT AGCCATCTGC AGAGCTTCCT 
               
               
                   
               
               
                 201 GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC TCTGGCGGCT 
               
               
                   
               
               
                 251 CTGGCGGCTC TCAGAGCTTC CTGCTCAAGT CTTTAGAGCA AGTGAGGAAG 
               
               
                   
               
               
                 301 ATCCAGGGCG ATGGCGCAGC GCTCCAGGAG AAGCTGTGTG CCACCTACAA 
               
               
                   
               
               
                 351 GCTGTGCCAC CCCGAGGAGC TGGTGCTGCT CGGACACTCT CTGGGCATCC 
               
               
                   
               
               
                 401 CCTGGGCTCC CCTGAGCTCC TGCCCCAGCC AGGCCCTGCA GCTGGCAGGC 
               
               
                   
               
               
                 451 TGCTTGAGCC AACTCCATAG CGGCCTTTTC CTCTACCAGG GGCTCCTGCA 
               
               
                   
               
               
                 501 GGCCCTGGAA GGGATATCCT AATAA 
               
               
                   
               
               
                 pMON3487.Seq 
               
               
                   
               
               
                   1 ATGGCTATGG CCCCTGCCCT GCAGCCCACC CAGGGTGCCA TGCCGGCCTT 
                 (SEQ ID NO:27) 
               
               
                   
               
               
                  51 CGCCTCTGCT TTCCAGCGCC GGGCAGGAGG GGTCCTGGTT GCTAGCCATC 
               
               
                   
               
               
                 101 TGCAGAGCTT CCTGGAGGTG TCGTACCGCG TTCTACGCCA CCTTGCGCAG 
               
               
                   
               
               
                 151 CCCTCTGGCG GCTCTGGCGG CTCTCAGAGC TTCCTGCTCA AGTCTTTAGA 
               
               
                   
               
               
                 201 GCAAGTGAGG AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT 
               
               
                   
               
               
                 251 GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT GCTCGGACAC 
               
               
                   
               
               
                 301 TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT 
               
               
                   
               
               
                 351 GCAGCTGGCA GGCTGCTTGA GCCAACTCCA TAGCGGCCTT TTCCTCTACC 
               
               
                   
               
               
                 401 AGGGGCTCCT GCAGGCCCTG GAAGGGATAT CCCCCGAGTT GGGTCCCACC 
               
               
                   
               
               
                 451 TTGGACACAC TGCAGCTGGA CGTCGCCGAC TTTGCCACCA CCATCTGGCA 
               
               
                   
               
               
                 501 GCAGATGGAA GAACTGGGAT AATAA 
               
               
                   
               
               
                 pMON3488.Seq 
               
               
                   
               
               
                   1 ATGGCTACCC AGGGTGCCAT GCCGGCCTTC GCCTCTGCTT TCCAGCGCCG 
                 (SEQ ID NO:28) 
               
               
                   
               
               
                  51 GGCAGGAGGG GTCCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT 
               
               
                   
               
               
                 101 CGTACCGCGT TCTACGCCAC CTTGCGCAGC CCTCTGGCGG CTCTGGCGGC 
               
               
                   
               
               
                 151 TCTCAGAGCT TCCTGCTCAA GTCTTTAGAG CAAGTGAGGA AGATCCAGGG 
               
               
                   
               
               
                 201 CGATGGCGCA GCGCTCCAGG AGAAGCTGTG TGCCACCTAC AAGCTGTGCC 
               
               
                   
               
               
                 251 ACCCCGAGGA GCTGGTGCTG CTCGGACACT CTCTGGGCAT CCCCTGGGCT 
               
               
                   
               
               
                 301 CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG 
               
               
                   
               
               
                 351 CCAACTCCAT AGCGGCCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG 
               
               
                   
               
               
                 401 AAGGGATATC CCCCGAGTTG GGTCCCACCT TGGACACACT GCAGCTGGAC 
               
               
                   
               
               
                 451 GTCGCCGACT TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGAAT 
               
               
                   
               
               
                 501 GGCCCCTGCC CTGCAGCCCT AATAA 
               
               
                   
               
               
                 pMON3489.Seq 
               
               
                   
               
               
                   1 ATGGCTTCTG CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA 
                 (SEQ ID NO:29) 
               
               
                   
               
               
                  51 TCTGCAGAGC TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC 
               
               
                   
               
               
                 101 AGCCCTCTGG CGGCTCTGGC GGCTCTCAGA GCTTCCTGCT CAAGTCTTTA 
               
               
                   
               
               
                 151 GAGCAAGTGA GGAAGATCCA GGGCGATGGC GCAGCGCTCC AGGAGAAGCT 
               
               
                   
               
               
                 201 GTGTGCCACC TACAAGCTGT GCCACCCCGA GGAGCTGGTG CTGCTCGGAC 
               
               
                   
               
               
                 251 ACTCTCTGGG CATCCCCTGG GCTCCCCTGA GCTCCTGCCC CAGCCAGGCC 
               
               
                   
               
               
                 301 CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA 
               
               
                   
               
               
                 351 CCAGGGGCTC CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA 
               
               
                   
               
               
                 401 CCTTGGACAC ACTGCAGCTG GACGTCGCCG ACTTTGCCAC CACCATCTGG 
               
               
                   
               
               
                 451 CAGCAGATGG AAGAACTGGG AATGGCCCCT GCCCTGCAGC CCACCCAGGG 
               
               
                   
               
               
                 501 TGCCATGCCG GCCTTCGCCT AATAA 
               
               
                   
               
               
                 pMON3490.seq 
               
               
                   
               
               
                   1 ATGGCTTACA AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC 
                 (SEQ ID NO:30) 
               
               
                   
               
               
                  51 TCTGGGCATC CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC 
               
               
                   
               
               
                 101 AGCTGGCAGG CTGCTTGAGC CAACTCCATA GCGGCCTTTT CCTCTACCAG 
               
               
                   
               
               
                 151 GGGCTCCTGC AGGCCCTGGA AGGGATATCC CCCGAGTTGG GTCCCACCTT 
               
               
                   
               
               
                 201 GGACACACTG CAGCTGGACG TCGCCGACTT TGCCACCACC ATCTGGCAGC 
               
               
                   
               
               
                 251 AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC 
               
               
                   
               
               
                 301 ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC CGGGCAGGAG GGGTCCTGGT 
               
               
                   
               
               
                 351 TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT GTCGTACCGC GTTCTACGCC 
               
               
                   
               
               
                 401 ACCTTGCGCA GCCCACACCA TTGGGCCCTG CCAGCTCCCT GCCCCAGAGC 
               
               
                   
               
               
                 451 TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA AAGATCCAGG GCGATGGCGC 
               
               
                   
               
               
                 501 AGCGCTCCAG GAGAAGCTGT GTGCCACCTA ATAA 
               
               
                   
               
               
                 pMON3491.seq 
               
               
                   
               
               
                   1 ATGGCTCCCG AGTTGGGTCC CACCTTGGAC ACACTGCAGC TGGACGTCGC 
                 (SEQ ID NO:31) 
               
               
                   
               
               
                  51 CGACTTTGCC ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC 
               
               
                   
               
               
                 101 CTGCCCTGCA GCCCACCCAG GGTGCCATGC CGGCCTTCGC CTCTGCTTTC 
               
               
                   
               
               
                 151 CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT AGCCATCTGC AGAGCTTCCT 
               
               
                   
               
               
                 201 GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC ACACCATTGG 
               
               
                   
               
               
                 251 GCCCTGCCAG CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA 
               
               
                   
               
               
                 301 GTGAGAAAGA TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC 
               
               
                   
               
               
                 351 CACCTACAAG CTGTGCCACC CCGAGGAGCT GGTGCTGCTC GGACACTCTC 
               
               
                   
               
               
                 401 TGGGCATCCC CTGGGCTCCC CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG 
               
               
                   
               
               
                 451 CTGGCAGGCT GCTTGAGCCA ACTCCATAGC GGCCTTTTCC TCTACCAGGG 
               
               
                   
               
               
                 501 GCTCCTGCAG GCCCTGGAAG GGATATCCTA ATAA 
               
               
                   
               
               
                 pMON3492.seq 
               
               
                   
               
               
                   1 ATGGCTATGG CCCCTGCCCT GCAGCCCACC CAGGGTGCCA TGCCGGCCTT 
                 (SEQ ID NO:32) 
               
               
                   
               
               
                  51 CGCCTCTGCT TTCCAGCGCC GGGCAGGAGG GGTCCTGGTT GCTAGCCATC 
               
               
                   
               
               
                 101 TGCAGAGCTT CCTGGAGGTG TCGTACCGCG TTCTACGCCA CCTTGCGCAG 
               
               
                   
               
               
                 151 CCCACACCAT TGGGCCCTGC CAGCTCCCTG CCCCAGAGCT TCCTGCTCAA 
               
               
                   
               
               
                 201 GTCTTTAGAG CAAGTGAGAA AGATCCAGGG CGATGGCGCA GCGCTCCAGG 
               
               
                   
               
               
                 251 CCCACACCAT TGGGCCCTGC CAGCTCCCTG CCCCAGAGCT TCCTGCTCAA 
               
               
                   
               
               
                 301 CTCGGACACT CTCTGGGCAT CCCCTGGGCT CCCCTGAGCT CCTGCCCCAG 
               
               
                   
               
               
                 351 CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG CCAACTCCAT AGCGGCCTTT 
               
               
                   
               
               
                 401 TCCTCTACCA GGGGCTCCTG CAGGCCCTGG AAGGGATATC CCCCGAGTTG 
               
               
                   
               
               
                 451 GGTCCCACCT TGGACACACT GCAGCTGGAC GTCGCCGACT TTGCCACCAC 
               
               
                   
               
               
                 501 CATCTGGCAG CAGATGGAAG AACTGGGATA ATAA 
               
               
                   
               
               
                 pMON3493.seq 
               
               
                   
               
               
                   1 ATGGCTACCC AGGGTGCCAT GCCGGCCTTC GCCTCTGCTT TCCAGCGCCG 
                 (SEQ ID NO:33) 
               
               
                   
               
               
                  51 GGCAGGAGGG GTCCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT 
               
               
                   
               
               
                 101 CGTACCGCGT TCTACGCCAC CTTGCGCAGC CCACACCATT GGGCCCTGCC 
               
               
                   
               
               
                 151 AGCTCCCTGC CCCAGAGCTT CCTGCTCAAG TCTTTAGAGC AAGTGAGAAA 
               
               
                   
               
               
                 201 GATCCAGGGC GATGGCGCAG CGCTCCAGGA GAAGCTGTGT GCCACCTACA 
               
               
                   
               
               
                 251 AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC TCTGGGCATC 
               
               
                   
               
               
                 301 CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG 
               
               
                   
               
               
                 351 CTGCTTGAGC CAACTCCATA GCGGCCTTTT CCTCTACCAG GGGCTCCTGC 
               
               
                   
               
               
                 401 AGGCCCTGGA AGGGATATCC CCCGAGTTGG GTCCCACCTT GGACACACTG 
               
               
                   
               
               
                 451 CAGCTGGACG TCGCCGACTT TGCCACCACC ATCTGGCAGC AGATGGAAGA 
               
               
                   
               
               
                 501 ACTGGGAATG GCCCCTGCCC TGCAGCCCTA ATAA 
               
               
                   
               
               
                 pMON3494.seq 
               
               
                   
               
               
                   1 ATGGCTTCTG CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA 
                 (SEQ ID NO:34) 
               
               
                   
               
               
                  51 TCTGCAGAGC TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC 
               
               
                   
               
               
                 101 AGCCCACACC ATTGGGCCCT GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC 
               
               
                   
               
               
                 151 AAGTCTTTAG AGCAAGTGAG AAAGATCCAG GGCGATGGCG CAGCGCTCCA 
               
               
                   
               
               
                 201 GGAGAAGCTG TGTGCCACCT ACAAGCTGTG CCACCCCGAG GAGCTGGTGC 
               
               
                   
               
               
                 251 TGCTCGGACA CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC 
               
               
                   
               
               
                 301 AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG AGCCAACTCC ATAGCGGCCT 
               
               
                   
               
               
                 351 TTTCCTCTAC CAGGGGCTCC TGCAGGCCCT GGAAGGGATA TCCCCCGAGT 
               
               
                   
               
               
                 401 TGGGTCCCAC CTTGGACACA CTGCAGCTGG ACGTCGCCGA CTTTGCCACC 
               
               
                   
               
               
                 451 ACCATCTGGC AGCAGATGGA AGAACTGGGA ATGGCCCCTG CCCTGCAGCC 
               
               
                   
               
               
                 501 CACCCAGGGT GCCATGCCGG CCTTCGCCTA ATAA 
               
               
                   
               
               
                 pMON25181.seq 
               
               
                   
               
               
                   1 ATGGCTCCGG AACTGGGTCC AACTCTGGAC ACACTGCAGC TGGACGTCGC 
                 (SEQ ID NO:35) 
               
               
                   
               
               
                  51 CGACTTTGCC ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC 
               
               
                   
               
               
                 101 CTGCCCTGCA GCCCACCCAG GGTGCCATGC CGGCCTTCGC CTCTGCTTTC 
               
               
                   
               
               
                 151 CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT AGCCATCTGC AGAGCTTCCT 
               
               
                   
               
               
                 201 GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC ACACCATTGG 
               
               
                   
               
               
                 251 GCCCTGCCAG CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA 
               
               
                   
               
               
                 301 GTGAGAAAGA TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC 
               
               
                   
               
               
                 351 CACCTACAAG CTGTGCCACC CCGAGGAGCT GGTGCTGCTC GGACACTCTC 
               
               
                   
               
               
                 401 TGGGCATCCC CTGGGCTCCC CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG 
               
               
                   
               
               
                 451 CTGGCAGGCT GCTTGAGCCA ACTCCATAGC GGCCTTTTCC TCTACCAGGG 
               
               
                   
               
               
                 501 GCTCCTGCAG GCCCTGGAAG GGATATCCTA A 
               
               
                   
               
               
                 pMON25182.seq 
               
               
                   
               
               
                   1 ATGGCTATGG CTCCAGCTCT GCAACCAACT CAAGGTGCAA TGCCAGCATT 
                 (SEQ ID NO:36) 
               
               
                   
               
               
                  51 TGCATCTGCT TTTCAACGTC GTGCAGGTGG TGTTCTGGTT GCTAGCCATC 
               
               
                   
               
               
                 101 TGCAGAGCTT CCTGGAGGTG TCGTACCGCG TTCTACGCCA CCTTGCGCAG 
               
               
                   
               
               
                 151 CCCACACCAT TGGGCCCTGC CAGCTCCCTG CCCCAGAGCT TCCTGCTCAA 
               
               
                   
               
               
                 201 GTCTTTAGAG CAAGTGAGAA AGATCCAGGG CGATGGCGCA GCGCTCCAGG 
               
               
                   
               
               
                 251 AGAAGCTGTG TGCCACCTAC AAGCTGTGCC ACCCCGAGGA GCTGGTGCTG 
               
               
                   
               
               
                 301 CTCGGACACT CTCTGGGCAT CCCCTGGGCT CCCCTGAGCT CCTGCCCCAG 
               
               
                   
               
               
                 351 CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG CCAACTCCAT AGCGGCCTTT 
               
               
                   
               
               
                 401 TCCTCTACCA GGGGCTCCTG CAGGCCCTGG AAGGGATATC CCCCGAGTTG 
               
               
                   
               
               
                 451 GGTCCCACCT TGGACACACT GCAGCTGGAC GTCGCCGACT TTGCCACCAC 
               
               
                   
               
               
                 501 CATCTGGCAG CAGATGGAAG AACTGGGATA A 
               
               
                   
               
               
                 pMON25183.seq 
               
               
                   
               
               
                   1 ATGGCTACTC AAGGTGCTAT GCCAGCTTTT GCTTCTGCTT TTCAACGTCG 
                 (SEQ ID NO:37) 
               
               
                   
               
               
                  51 TGCAGGTGGT GTTCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT 
               
               
                   
               
               
                 101 CGTACCGCGT TCTACGCCAC CTTGCGCAGC CCACACCATT GGGCCCTGCC 
               
               
                   
               
               
                 151 AGCTCCCTGC CCCAGAGCTT CCTGCTCAAG TCTTTAGAGC AAGTGAGAAA 
               
               
                   
               
               
                 201 GATCCAGGGC GATGGCGCAG CGCTCCAGGA GAAGCTGTGT GCCACCTACA 
               
               
                   
               
               
                 251 AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC TCTGGGCATC 
               
               
                   
               
               
                 301 CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG 
               
               
                   
               
               
                 351 CTGCTTGAGC CAACTCCATA GCGGCCTTTT CCTCTACCAG GGGCTCCTGC 
               
               
                   
               
               
                 401 AGGCCCTGGA AGGGATATCC CCCGAGTTGG GTCCCACCTT GGACACACTG 
               
               
                   
               
               
                 451 CAGCTGGACG TCGCCGACTT TGCCACCACC ATCTGGCAGC AGATGGAAGA 
               
               
                   
               
               
                 501 ACTGGGAATG GCCCCTGCCC TGCAGCCCTA A 
               
               
                   
               
               
                 pMON25184.seq 
               
               
                   
               
               
                   1 ATGGCTTCTG CTTTTCAACG TCGTGCAGGT GGTGTTCTGG TTGCTAGCCA 
                 (SEQ ID NO:38) 
               
               
                   
               
               
                  51 TCTGCAGAGC TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC 
               
               
                   
               
               
                 101 AGCCCACACC ATTGGGCCCT GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC 
               
               
                   
               
               
                 151 AAGTCTTTAG AGCAAGTGAG AAAGATCCAG GGCGATGGCG CAGCGCTCCA 
               
               
                   
               
               
                 201 GGAGAAGCTG TGTGCCACCT ACAAGCTGTG CCACCCCGAG GAGCTGGTGC 
               
               
                   
               
               
                 251 TGCTCGGACA CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC 
               
               
                   
               
               
                 301 AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG AGCCAACTCC ATAGCGGCCT 
               
               
                   
               
               
                 351 TTTCCTCTAC CAGGGGCTCC TGCAGGCCCT GGAAGGGATA TCCCCCGAGT 
               
               
                   
               
               
                 401 TGGGTCCCAC CTTGGACACA CTGCAGCTGG ACGTCGCCGA CTTTGCCACC 
               
               
                   
               
               
                 451 ACCATCTGGC AGCAGATGGA AGAACTGGGA ATGGCCCCTG CCCTGCAGCC 
               
               
                   
               
               
                 501 CACCCAGGGT GCCATGCCGG CCTTCGCCTA A 
               
               
                   
               
               
                   
               
               
                 pMON25185.seq 
               
               
                   
               
               
                   1 ATGGCTCCGG AACTGGGTCC AACTCTGGAC ACACTGCAGC TGGACGTCGC 
                 (SEQ ID NO:39) 
               
               
                   
               
               
                  51 CGACTTTGCC ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC 
               
               
                   
               
               
                 101 CTGCCCTGCA GCCCACCCAG GGTGCCATGC CGGCCTTCGC CTCTGCTTTC 
               
               
                   
               
               
                 151 CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT AGCCATCTGC AGAGCTTCCT 
               
               
                   
               
               
                 201 GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC TCTGGCGGCT 
               
               
                   
               
               
                 251 CTGGCGGCTC TCAGAGCTTC CTGCTCAAGT CTTTAGAGCA AGTGAGAAAG 
               
               
                   
               
               
                 301 ATCCAGGGCG ATGGCGCAGC GCTCCAGGAG AAGCTGTGTG CCACCTACAA 
               
               
                   
               
               
                 351 GCTGTGCCAC CCCGAGGAGC TGGTGCTGCT CGGACACTCT CTGGGCATCC 
               
               
                   
               
               
                 401 CCTGGGCTCC CCTGAGCTCC TGCCCCAGCC AGGCCCTGCA GCTGGCAGGC 
               
               
                   
               
               
                 451 TGCTTGAGCC AACTCCATAG CGGCCTTTTC CTCTACCAGG GGCTCCTGCA 
               
               
                   
               
               
                 501 GGCCCTGGAA GGGATATCCT AA 
               
               
                   
               
               
                   
               
               
                 pMON25186.seq 
               
               
                   
               
               
                   1 ATGGCTATGG CTCCAGCTCT GCAACCAACT CAAGGTGCAA TGCCAGCATT 
                 (SEQ ID NO:40) 
               
               
                   
               
               
                  51 TGCATCTGCT TTTCAACGTC GTGCAGGTGG TGTTCTGGTT GCTAGCCATC 
               
               
                   
               
               
                 101 TGCAGAGCTT CCTGGAGGTG TCGTACCGCG TTCTACGCCA CCTTGCGCAG 
               
               
                   
               
               
                 151 CCCTCTGGCG GCTCTGGCGG CTCTCAGAGC TTCCTGCTCA AGTCTTTAGA 
               
               
                   
               
               
                 201 GCAAGTGAGA AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT 
               
               
                   
               
               
                 251 GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT GCTCGGACAC 
               
               
                   
               
               
                 301 TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT 
               
               
                   
               
               
                 351 GCAGCTGGCA GGCTGCTTGA GCCAACTCCA TAGCGGCCTT TTCCTCTACC 
               
               
                   
               
               
                 401 AGGGGCTCCT GCAGGCCCTG GAAGGGATAT CCCCCGAGTT GGGTCCCACC 
               
               
                   
               
               
                 451 TTGGACACAC TGCAGCTGGA CGTCGCCGAC TTTGCCACCA CCATCTGGCA 
               
               
                   
               
               
                 501 GCAGATGGAA GAACTGGGAT AA 
               
               
                   
               
               
                   
               
               
                 pMON25187.seq 
               
               
                   
               
               
                   1 ATGGCTACTC AAGGTGCTAT GCCAGCTTTT GCTTCTGCTT TTCAACGTCG 
                 (SEQ ID NO:41) 
               
               
                   
               
               
                  51 TGCAGGTGGT GTTCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT 
               
               
                   
               
               
                 101 CGTACCGCGT TCTACGCCAC CTTGCGCAGC CCTCTGGCGG CTCTGGCGGC 
               
               
                   
               
               
                 151 TCTCAGAGCT TCCTGCTCAA GTCTTTAGAG CAAGTGAGAA AGATCCAGGG 
               
               
                   
               
               
                 201 CGATGGCGCA GCGCTCCAGG AGAAGCTGTG TGCCACCTAC AAGCTGTGCC 
               
               
                   
               
               
                 251 ACCCCGAGGA GCTGGTGCTG CTCGGACACT CTCTGGGCAT CCCCTGGGCT 
               
               
                   
               
               
                 301 CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG 
               
               
                   
               
               
                 351 CCAACTCCAT AGCGGCCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG 
               
               
                   
               
               
                 401 AAGGGATATC CCCCGAGTTG GGTCCCACCT TGGACACACT GCAGCTGGAC 
               
               
                   
               
               
                 451 GTCGCCGACT TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGAAT 
               
               
                   
               
               
                 501 GGCCCCTGCC CTGCAGCCCT AA 
               
               
                   
               
               
                 pMON25188.seq 
               
               
                   
               
               
                   1 ATGGCTTCTG CTTTTCAACG TCGTGCAGGT GGTGTTCTGG TTGCTAGCCA 
                 (SEQ ID NO:42) 
               
               
                   
               
               
                  51 TCTGCAGAGC TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC 
               
               
                   
               
               
                 101 AGCCCTCTGG CGGCTCTGGC GGCTCTCAGA GCTTCCTGCT CAAGTCTTTA 
               
               
                   
               
               
                 151 GAGCAAGTGA GAAAGATCCA GGGCGATGGC GCAGCGCTCC AGGAGAAGCT 
               
               
                   
               
               
                 201 GTGTGCCACC TACAAGCTGT GCCACCCCGA GGAGCTGGTG CTGCTCGGAC 
               
               
                   
               
               
                 251 ACTCTCTGGG CATCCCCTGG GCTCCCCTGA GCTCCTGCCC CAGCCAGGCC 
               
               
                   
               
               
                 301 CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA 
               
               
                   
               
               
                 351 CCAGGGGCTC CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA 
               
               
                   
               
               
                 401 CCTTGGACAC ACTGCAGCTG GACGTCGCCG ACTTTGCCAC CACCATCTGG 
               
               
                   
               
               
                 451 CAGCAGATGG AAGAACTGGG AATGGCCCCT GCCCTGCAGC CCACCCAGGG 
               
               
                   
               
               
                 501 TGCCATGCCG GCCTTCGCCT AA 
               
               
                   
               
               
                 pMON3460.seq 
               
               
                   
               
               
                   1 ATGGCTCTGC TCGGACACTC TCTGGGCATC CCCTGGGCTC CCCTGAGCTC 
                 (SEQ ID NO:86) 
               
               
                   
               
               
                  51 CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC CAACTCCATA 
               
               
                   
               
               
                 101 GCGGCCTTTT CCTCTACCAG GGGCTCCTGC AGGCCCTGGA AGGGATATCC 
               
               
                   
               
               
                 151 CCCGAGTTGG GTCCCACCTT GGACACACTG CAGCTGGACG TCGCCGACTT 
               
               
                   
               
               
                 201 TGCCACCACC ATCTGGCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC 
               
               
                   
               
               
                 251 TGCAGCCCAC CCAGGGTGCC ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC 
               
               
                   
               
               
                 301 CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT 
               
               
                   
               
               
                 351 GTCGTACCGC GTTCTACGCC ACCTTGCGCA GCCCACACCA TTGGGCCCTG 
               
               
                   
               
               
                 401 CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA 
               
               
                   
               
               
                 451 AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA 
               
               
                   
               
               
                 501 CAAGCTGTGC CACCCCGAGG AGCTGGTGTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3461.seq 
               
               
                   
               
               
                   1 ATGGCTCAAC TCCATAGCGG CCTTTTCCTC TACCAGGGGC TCCTGCAGGC 
                 (SEQ ID NO:87) 
               
               
                   
               
               
                  51 CCTGGAAGGG ATATCCCCCG AGTTGGGTCC CACCTTGGAC ACACTGCAGC 
               
               
                   
               
               
                 101 TGGACGTCGC CGACTTTGCC ACCACCATCT GGCAGCAGAT GGAAGAACTG 
               
               
                   
               
               
                 151 GGAATGGCCC CTGCCCTGCA GCCCACCCAG GGTGCCATGC CGGCCTTCGC 
               
               
                   
               
               
                 201 CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT AGCCATCTGC 
               
               
                   
               
               
                 251 AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC 
               
               
                   
               
               
                 301 ACACCATTGG GCCCTGCCAG CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC 
               
               
                   
               
               
                 351 TTTAGAGCAA GTGAGAAAGA TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA 
               
               
                   
               
               
                 401 AGCTGTGTGC CACCTACAAG CTGTGCCACC CCGAGGAGCT GGTGCTGCTC 
               
               
                   
               
               
                 451 GGACACTCTC TGGGCATCCC CTGGGCTCCC CTGAGCTCCT GCCCCAGCCA 
               
               
                   
               
               
                 501 GGCCCTGCAG CTGGCAGGCT GCTTGAGCTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3462.seq 
               
               
                   
               
               
                   1 ATGGCTCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG AAGGGATATC 
                 (SEQ ID NO:88) 
               
               
                   
               
               
                  51 CCCCGAGTTG GGTCCCACCT TGGACACACT GCAGCTGGAC GTCGCCGACT 
               
               
                   
               
               
                 101 TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGAAT GGCCCCTGCC 
               
               
                   
               
               
                 151 CTGCAGCCCA CCCAGGGTGC CATGCCGGCC TTCGCCTCTG CTTTCCAGCG 
               
               
                   
               
               
                 201 CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC TTCCTGGAGG 
               
               
                   
               
               
                 251 TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCACACC ATTGGGCCCT 
               
               
                   
               
               
                 301 GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC AAGTCTTTAG AGCAAGTGAG 
               
               
                   
               
               
                 351 AAAGATCCAG GGCGATGGCG CAGCGCTCCA GGAGAAGCTG TGTGCCACCT 
               
               
                   
               
               
                 401 ACAAGCTGTG CCACCCCGAG GAGCTGGTGC TGCTCGGACA CTCTCTGGGC 
               
               
                   
               
               
                 451 ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC AGCCAGGCCC TGCAGCTGGC 
               
               
                   
               
               
                 501 AGGCTGCTTG AGCCAACTCC ATAGCGGCTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3463.seq 
               
               
                   
               
               
                   1 ATGGCTCTCT ACCAGGGGCT CCTGCAGGCC CTGGAAGGGA TATCCCCCGA 
                 (SEQ ID NO:89) 
               
               
                   
               
               
                  51 GTTGGGTCCC ACCTTGGACA CACTGCAGCT GGACGTCGCC GACTTTGCCA 
               
               
                   
               
               
                 101 CCACCATCTG GCAGCAGATG GAAGAACTGG GAATGGCCCC TGCCCTGCAG 
               
               
                   
               
               
                 151 CCCACCCAGG GTGCCATGCC GGCCTTCGCC TCTGCTTTCC AGCGCCGGGC 
               
               
                   
               
               
                 201 AGGAGGGGTC CTGGTTGCTA GCCATCTGCA GAGCTTCCTG GAGGTGTCGT 
               
               
                   
               
               
                 251 ACCGCGTTCT ACGCCACCTT GCGCAGCCCA CACCATTGGG CCCTGCCAGC 
               
               
                   
               
               
                 301 TCCCTGCCCC AGAGCTTCCT GCTCAAGTCT TTAGAGCAAG TGAGAAAGAT 
               
               
                   
               
               
                 351 CCAGGGCGAT GGCGCAGCGC TCCAGGAGAA GCTGTGTGCC ACCTACAAGC 
               
               
                   
               
               
                 401 TGTGCCACCC CGAGGAGCTG GTGCTGCTCG GACACTCTCT GGGCATCCCC 
               
               
                   
               
               
                 451 TGGGCTCCCC TGAGCTCCTG CCCCAGCCAG GCCCTGCAGC TGGCAGGCTG 
               
               
                   
               
               
                 501 CTTGAGCCAA CTCCATAGCG GCCTTTTCTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3464.seq 
               
               
                   
               
               
                   1 ATGGCTGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA CCTTGGACAC 
                 (SEQ ID NO:90) 
               
               
                   
               
               
                  51 ACTGCAGCTG GACGTCGCCG ACTTTGCCAC CACCATCTGG CAGCAGATGG 
               
               
                   
               
               
                 101 AAGAACTGGG AATGGCCCCT GCCCTGCAGC CCACCCAGGG TGCCATGCCG 
               
               
                   
               
               
                 151 GCCTTCGCCT CTGCTTTCCA GCGCCGGGCA GGAGGGGTCC TGGTTGCTAG 
               
               
                   
               
               
                 201 CCATCTGCAG AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA CGCCACCTTG 
               
               
                   
               
               
                 251 CGCAGCCCAC ACCATTGGGC CCTGCCAGCT CCCTGCCCCA GAGCTTCCTG 
               
               
                   
               
               
                 301 CTCAAGTCTT TAGAGCAAGT GAGAAAGATC CAGGGCGATG GCGCAGCGCT 
               
               
                   
               
               
                 351 CCAGGAGAAG CTGTGTGCCA CCTACAAGCT GTGCCACCCC GAGGAGCTGG 
               
               
                   
               
               
                 401 TGCTGCTCGG ACACTCTCTG GGCATCCCCT GGGCTCCCCT GAGCTCCTGC 
               
               
                   
               
               
                 451 CCCAGCCAGG CCCTGCAGCT GGCAGGCTGC TTGAGCCAAC TCCATAGCGG 
               
               
                   
               
               
                 501 CCTTTTCCTC TACCAGGGGC TCCTGCAGTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3465.seq 
               
               
                   
               
               
                   1 ATGGCTGACT TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGAAT 
                 (SEQ ID NO:91) 
               
               
                   
               
               
                  51 GGCCCCTGCC CTGCAGCCCA CCCAGGGTGC CATGCCGGCC TTCGCCTCTG 
               
               
                   
               
               
                 101 CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC 
               
               
                   
               
               
                 151 TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCACACC 
               
               
                   
               
               
                 201 ATTGGGCCCT GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC AAGTCTTTAG 
               
               
                   
               
               
                 251 AGCAAGTGAG AAAGATCCAG GGCGATGGCG CAGCGCTCCA GGAGAAGCTG 
               
               
                   
               
               
                 301 TGTGCCACCT ACAAGCTGTG CCACCCCGAG GAGCTGGTGC TGCTCGGACA 
               
               
                   
               
               
                 351 CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC AGCCAGGCCC 
               
               
                   
               
               
                 401 TGCAGCTGGC AGGCTGCTTG AGCCAACTCC ATAGCGGCCT TTTCCTCTAC 
               
               
                   
               
               
                 451 CAGGGGCTCC TGCAGGCCCT GGAAGGGATA TCCCCCGAGT TGGGTCCCAC 
               
               
                   
               
               
                 501 CTTGGACACA CTGCAGCTGG ACGTCGCCTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3466.seq 
               
               
                   
               
               
                   1 ATGGCTATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC CTGCCCTGCA 
                 (SEQ ID NO:92) 
               
               
                   
               
               
                  51 GCCCACCCAG GGTGCCATGC CGGCCTTCGC CTCTGCTTTC CAGCGCCGGG 
               
               
                   
               
               
                 101 CAGGAGGGGT CCTGGTTGCT AGCCATCTGC AGAGCTTCCT GGAGGTGTCG 
               
               
                   
               
               
                 151 TACCGCGTTC TACGCCACCT TGCGCAGCCC ACACCATTGG GCCCTGCCAG 
               
               
                   
               
               
                 201 CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA GTGAGAAAGA 
               
               
                   
               
               
                 251 TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC CACCTACAAG 
               
               
                   
               
               
                 301 CTGTGCCACC CCGAGGAGCT GGTGCTGCTC GGACACTCTC TGGGCATCCC 
               
               
                   
               
               
                 351 CTGGGCTCCC CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG CTGGCAGGCT 
               
               
                   
               
               
                 401 GCTTGAGCCA ACTCCATAGC GGCCTTTTCC TCTACCAGGG GCTCCTGCAG 
               
               
                   
               
               
                 451 GCCCTGGAAG GGATATCCCC CGAGTTGGGT CCCACCTTGG ACACACTGCA 
               
               
                   
               
               
                 501 GCTGGACGTC GCCGACTTTG CCACCACCTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3467.seq 
               
               
                   
               
               
                   1 ATGGCTCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCAC 
                 (SEQ ID NO:93) 
               
               
                   
               
               
                  51 CCAGGGTGCC ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC CGGGCAGGAG 
               
               
                   
               
               
                 101 GGGTCCTGGT TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT GTCGTACCGC 
               
               
                   
               
               
                 151 GTTCTACGCC ACCTTGCGCA GCCCACACCA TTGGGCCCTG CCAGCTCCCT 
               
               
                   
               
               
                 201 GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA AAGATCCAGG 
               
               
                   
               
               
                 251 GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA CAAGCTGTGC 
               
               
                   
               
               
                 301 CACCCCGAGG AGCTGGTGCT GCTCGGACAC TCTCTGGGCA TCCCCTGGGC 
               
               
                   
               
               
                 351 TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA GGCTGCTTGA 
               
               
                   
               
               
                 401 GCCAACTCCA TAGCGGCCTT TTCCTCTACC AGGGGCTCCT GCAGGCCCTG 
               
               
                   
               
               
                 451 GAAGGGATAT CCCCCGAGTT GGGTCCCACC TTGGACACAC TGCAGCTGGA 
               
               
                   
               
               
                 501 CGTCGCCGAC TTTGCCACCA CCATCTGGTA ATAA 
               
               
                   
               
               
                   
               
               
                 pMON3499.seq 
               
               
                   
               
               
                   1 ATGGCTTTGT TAGGACATTC TTTAGGTATT CCATGGGCTC CTCTGAGCTC 
                 (SEQ ID NO:94) 
               
               
                   
               
               
                  51 CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC CAACTCCATA 
               
               
                   
               
               
                 101 GCGGCCTTTT CCTCTACCAG GGGCTCCTGC AGGCCCTGGA AGGGATATCC 
               
               
                   
               
               
                 151 CCCGAGTTGG GTCCCACCTT GGACACACTG CAGCTGGACG TCGCCGACTT 
               
               
                   
               
               
                 201 TGCCACCACC ATCTGGCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC 
               
               
                   
               
               
                 251 TGCAGCCCAC CCAGGGTGCC ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC 
               
               
                   
               
               
                 301 CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT 
               
               
                   
               
               
                 351 GTCGTACCGC GTTCTACGCC ACCTTGCGCA GCCCACACCA TTGGGCCCTG 
               
               
                   
               
               
                 401 CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA 
               
               
                   
               
               
                 451 AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA 
               
               
                   
               
               
                 501 CAAGCTGTGC CACCCCGAGG AGCTGGTGTA ATAA 
               
               
                   
               
               
                   
               
               
                 pG1110.Seq 
               
               
                   
               
               
                   1 CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA GTGAGGAAGA TCCAGGGCGA 
                 (SEQ ID NO:116) 
               
               
                   
               
               
                  51 TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC CACCTACAAG CTGTGCCACC 
               
               
                   
               
               
                 101 CCGAGGAGCT GGTGCTGCTC GGACACTCTC TGGGCATCCC CTGGGCTCCC 
               
               
                   
               
               
                 151 CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG CTGGCAGGCT GCTTGAGCCA 
               
               
                   
               
               
                 201 ACTCCATAGC GGCCTTTTCC TCTACCAGGG GCTCCTGCAG GCCCTGGAAG 
               
               
                   
               
               
                 251 GGATATCCCC CGAGTTGGGT CCCACCTTGG ACACACTGCA GCTGGACGTC 
               
               
                   
               
               
                 301 GCCGACTTTG CCACCACCAT CTGGCAGCAG ATGGAAGAAC TGGGAATGGC 
               
               
                   
               
               
                 351 CCCTGCCCTG CAGCCCACCC AGGGTGCCAT GCCGGCCTTC GCCTCTGCTT 
               
               
                   
               
               
                 401 TCCAGCGCCG GGCAGGAGGG GTCCTGGTTG CTAGCCATCT GCAGAGCTTC 
               
               
                   
               
               
                 451 CTGGAGGTGT CGTACCGCGT TCTACGCCAC CTTGCGCAGC CCGACATGGC 
               
               
                   
               
               
                 501 TACACCATTA GGCCCTGCCA GCTCCCTGCC C 
               
               
                   
               
               
                 pG123122.Seq 
               
               
                   
               
               
                   1 GAACTGGGAA TGGCCCCTGC CCTGCAGCCC ACCCAGGGTG CCATGCCGGC 
                 (SEQ ID NO:117) 
               
               
                   
               
               
                  51 CTTCGCCTCT GCTTTCCAGC GCCGGGCAGG AGGGGTCCTG GTTGCTAGCC 
               
               
                   
               
               
                 101 ATCTGCAGAG CTTCCTGGAG GTGTCGTACC GCGTTCTACG CCACCTTGCG 
               
               
                   
               
               
                 151 CAGCCCGACA TGGCTACACC ATTAGGCCCT GCCAGCTCCC TGCCCCAGAG 
               
               
                   
               
               
                 201 CTTCCTGCTC AAGTCTTTAG AGCAAGTGAG GAAGATCCAG GGCGATGGCG 
               
               
                   
               
               
                 251 CAGCGCTCCA GGAGAAGCTG TGTGCCACCT ACAAGCTGTG CCACCCCGAG 
               
               
                   
               
               
                 301 GAGCTGGTGC TGCTCGGACA CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG 
               
               
                   
               
               
                 351 CTCCTGCCCC AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG AGCCAACTCC 
               
               
                   
               
               
                 401 ATAGCGGCCT TTTCCTCTAC CAGGGGCTCC TGCAGGCCCT GGAAGGGATA 
               
               
                   
               
               
                 451 TCCCCCGAGT TGGGTCCCAC CTTGGACACA CTGCAGCTGG ACGTCGCCGA 
               
               
                   
               
               
                 501 CTTTGCCACC ACCATCTGGC AGCAGATGGA A 
               
               
                   
               
               
                 pG125124.Seq 
               
               
                   
               
               
                   1 GGAATGGCCC CTGCCCTGCA GCCCACCCAG GGTGCCATGC CGGCCTTCGC 
                 (SEQ ID NO:118) 
               
               
                   
               
               
                  51 CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT AGCCATCTGC 
               
               
                   
               
               
                 101 AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC 
               
               
                   
               
               
                 151 GACATGGCTA CACCATTAGG CCCTGCCAGC TCCCTGCCCC AGAGCTTCCT 
               
               
                   
               
               
                 201 GCTCAAGTCT TTAGAGCAAG TGAGGAAGAT CCAGGGCGAT GGCGCAGCGC 
               
               
                   
               
               
                 251 TCCAGGAGAA GCTGTGTGCC ACCTACAAGC TGTGCCACCC CGAGGAGCTG 
               
               
                   
               
               
                 301 GTGCTGCTCG GACACTCTCT GGGCATCCCC TGGGCTCCCC TGAGCTCCTG 
               
               
                   
               
               
                 351 CCCCAGCCAG GCCCTGCAGC TGGCAGGCTG CTTGAGCCAA CTCCATAGCG 
               
               
                   
               
               
                 401 GCCTTTTCCT CTACCAGGGG CTCCTGCAGG CCCTGGAAGG GATATCCCCC 
               
               
                   
               
               
                 451 GAGTTGGGTC CCACCTTGGA CACACTGCAG CTGGACGTCG CCGACTTTGC 
               
               
                   
               
               
                 501 CACCACCATC TGGCAGCAGA TGGAAGAACT G 
               
               
                   
               
               
                 pG1312.Seq 
               
               
                   
               
               
                   1 TTCCTGCTCA AGTCTTTAGA GCAAGTGAGG AAGATCCAGG GCGATGGCGC 
                 (SEQ ID NO:119) 
               
               
                   
               
               
                  51 AGCGCTCCAG GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG 
               
               
                   
               
               
                 101 AGCTGGTGCT GCTCGGACAC TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC 
               
               
                   
               
               
                 151 TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA GGCTGCTTGA GCCAACTCCA 
               
               
                   
               
               
                 201 TAGCGGCCTT TTCCTCTACC AGGGGCTCCT GCAGGCCCTG GAAGGGATAT 
               
               
                   
               
               
                 251 CCCCCGAGTT GGGTCCCACC TTGGACACAC TGCAGCTGGA CGTCGCCGAC 
               
               
                   
               
               
                 301 TTTGCCACCA CCATCTGGCA GCAGATGGAA GAACTGGGAA TGGCCCCTGC 
               
               
                   
               
               
                 351 CCTGCAGCCC ACCCAGGGTG CCATGCCGGC CTTCGCCTCT GCTTTCCAGC 
               
               
                   
               
               
                 401 GCCGGGCAGG AGGGGTCCTG GTTGCTAGCC ATCTGCAGAG CTTCCTGGAG 
               
               
                   
               
               
                 451 GTGTCGTACC GCGTTCTACG CCACCTTGCG CAGCCCGACA TGGCTACACC 
               
               
                   
               
               
                 501 ATTAGGCCCT GCCAGCTCCC TGCCCCAGAG C 
               
               
                   
               
               
                 pG159158.Seq 
               
               
                   
               
               
                   1 AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA CGCCACCTTG CGCAGCCCGA 
                 (SEQ ID NO:120) 
               
               
                   
               
               
                  51 CATGGCTACA CCATTAGGCC CTGCCAGCTC CCTGCCCCAG AGCTTCCTGC 
               
               
                   
               
               
                 101 TCAAGTCTTT AGAGCAAGTG AGGAAGATCC AGGGCGATGG CGCAGCGCTC 
               
               
                   
               
               
                 151 CAGGAGAAGC TGTGTGCCAC CTACAAGCTG TGCCACCCCG AGGAGCTGGT 
               
               
                   
               
               
                 201 GCTGCTCGGA CACTCTCTGG GCATCCCCTG GGCTCCCCTG AGCTCCTGCC 
               
               
                   
               
               
                 251 CCAGCCAGGC CCTGCAGCTG GCAGGCTGCT TGAGCCAACT CCATAGCGGC 
               
               
                   
               
               
                 301 CTTTTCCTCT ACCAGGGGCT CCTGCAGGCC CTGGAAGGGA TATCCCCCGA 
               
               
                   
               
               
                 351 GTTGGGTCCC ACCTTGGACA CACTGCAGCT GGACGTCGCC GACTTTGCCA 
               
               
                   
               
               
                 401 CCACCATCTG GCAGCAGATG GAAGAACTGG GAATGGCCCC TGCCCTGCAG 
               
               
                   
               
               
                 451 CCCACCCAGG GTGCCATGCC GGCCTTCGCC TCTGCTTTCC AGCGCCGGGC 
               
               
                   
               
               
                 501 AGGAGGGGTC CTGGTTGCTA GCCATCTGCA G 
               
               
                   
               
               
                 pG1918.Seq 
               
               
                   
               
               
                   1 GAGCAAGTGA GGAAGATCCA GGGCGATGGC GCAGCGCTCC AGGAGAAGCT 
                 (SEQ ID NO:121) 
               
               
                   
               
               
                  51 GTGTGCCACC TACAAGCTGT GCCACCCCGA GGAGCTGGTG CTGCTCGGAC 
               
               
                   
               
               
                 101 ACTCTCTGGG CATCCCCTGG GCTCCCCTGA GCTCCTGCCC CAGCCAGGCC 
               
               
                   
               
               
                 151 CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA 
               
               
                   
               
               
                 201 CCAGGGGCTC CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA 
               
               
                   
               
               
                 251 CCTTGGACAC ACTGCAGCTG GACGTCGCCG ACTTTGCCAC CACCATCTGG 
               
               
                   
               
               
                 301 CAGCAGATGG AAGAACTGGG AATGGCCCCT GCCCTGCAGC CCACCCAGGG 
               
               
                   
               
               
                 351 TGCCATGCCG GCCTTCGCCT CTGCTTTCCA GCGCCGGGCA GGAGGGGTCC 
               
               
                   
               
               
                 401 TGGTTGCTAG CCATCTGCAG AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA 
               
               
                   
               
               
                 451 CGCCACCTTG CGCAGCCCGA CATGGCTACA CCATTAGGCC CTGCCAGCTC 
               
               
                   
               
               
                 501 CCTGCCCCAG AGCTTCCTGC TCAAGTCTTT A 
               
               
                   
               
               
                 pG32.Seq 
               
               
                   
               
               
                   1 TTAGGCCCTG CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA 
                 (SEQ ID NO:122) 
               
               
                   
               
               
                  51 GCAAGTGAGG AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT 
               
               
                   
               
               
                 101 GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT GCTCGGACAC 
               
               
                   
               
               
                 151 TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT 
               
               
                   
               
               
                 201 GCAGCTGGCA GGCTGCTTGA GCCAACTCCA TAGCGGCCTT TTCCTCTACC 
               
               
                   
               
               
                 251 AGGGGCTCCT GCAGGCCCTG GAAGGGATAT CCCCCGAGTT GGGTCCCACC 
               
               
                   
               
               
                 301 TTGGACACAC TGCAGCTGGA CGTCGCCGAC TTTGCCACCA CCATCTGGCA 
               
               
                   
               
               
                 351 GCAGATGGAA GAACTGGGAA TGGCCCCTGC CCTGCAGCCC ACCCAGGGTG 
               
               
                   
               
               
                 401 CCATGCCGGC CTTCGCCTCT GCTTTCCAGC GCCGGGCAGG AGGGGTCCTG 
               
               
                   
               
               
                 451 GTTGCTAGCC ATCTGCAGAG CTTCCTGGAG GTGTCGTACC GCGTTCTACG 
               
               
                   
               
               
                 501 CCACCTTGCG CAGCCCGACA TGGCTACACC A 
               
               
                   
               
               
                 pG4948.Seq 
               
               
                   
               
               
                   1 CTGCTCGGAC ACTCTCTGGG CATCCCCTGG GCTCCCCTGA GCTCCTGCCC 
                 (SEQ ID NO:123) 
               
               
                   
               
               
                  51 CAGCCAGGCC CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC 
               
               
                   
               
               
                 101 TTTTCCTCTA CCAGGGGCTC CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG 
               
               
                   
               
               
                 151 TTGGGTCCCA CCTTGGACAC ACTGCAGCTG GACGTCGCCG ACTTTGCCAC 
               
               
                   
               
               
                 201 CACCATCTGG CAGCAGATGG AAGAACTGGG AATGGCCCCT GCCCTGCAGC 
               
               
                   
               
               
                 251 CCACCCAGGG TGCCATGCCG GCCTTCGCCT CTGCTTTCCA GCGCCGGGCA 
               
               
                   
               
               
                 301 GGAGGGGTCC TGGTTGCTAG CCATCTGCAG AGCTTCCTGG AGGTGTCGTA 
               
               
                   
               
               
                 351 CCGCGTTCTA CGCCACCTTG CGCAGCCCGA CATGGCTACA CCATTAGGCC 
               
               
                   
               
               
                 401 CTGCCAGCTC CCTGCCCCAG AGCTTCCTGC TCAAGTCTTT AGAGCAAGTG 
               
               
                   
               
               
                 451 AGGAAGATCC AGGGCGATGG CGCAGCGCTC CAGGAGAAGC TGTGTGCCAC 
               
               
                   
               
               
                 501 CTACAAGCTG TGCCACCCCG AGGAGCTGGT G 
               
               
                   
               
               
                 pG6059.Seq 
               
               
                   
               
               
                   1 CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG 
                 (SEQ ID NO:124) 
               
               
                   
               
               
                  51 CCAACTCCAT AGCGGCCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG 
               
               
                   
               
               
                 101 AAGGGATATC CCCCGAGTTG GGTCCCACCT TGGACACACT GCAGCTGGAC 
               
               
                   
               
               
                 151 GTCGCCGACT TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGAAT 
               
               
                   
               
               
                 201 GGCCCCTGCC CTGCAGCCCA CCCAGGGTGC CATGCCGGCC TTCGCCTCTG 
               
               
                   
               
               
                 251 CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC 
               
               
                   
               
               
                 301 TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCGACAT 
               
               
                   
               
               
                 351 GGCTACACCA TTAGGCCCTG CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA 
               
               
                   
               
               
                 401 AGTCTTTAGA GCAAGTGAGG AAGATCCAGG GCGATGGCGC AGCGCTCCAG 
               
               
                   
               
               
                 451 GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT 
               
               
                   
               
               
                 501 GCTCGGACAC TCTCTGGGCA TCCCCTGGGC T 
               
               
                   
               
               
                 pG6766.Seq 
               
               
                   
               
               
                   1 CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC CAACTCCATA GCGGCCTTTT 
                 (SEQ ID NO:125) 
               
               
                   
               
               
                  51 CCTCTACCAG GGGCTCCTGC AGGCCCTGGA AGGGATATCC CCCGAGTTGG 
               
               
                   
               
               
                 101 GTCCCACCTT GGACACACTG CAGCTGGACG TCGCCGACTT TGCCACCACC 
               
               
                   
               
               
                 151 ATCTGGCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCAC 
               
               
                   
               
               
                 201 CCAGGGTGCC ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC CGGGCAGGAG 
               
               
                   
               
               
                 251 GGGTCCTGGT TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT GTCGTACCGC 
               
               
                   
               
               
                 301 GTTCTACGCC ACCTTGCGCA GCCCGACATG GCTACACCAT TAGGCCCTGC 
               
               
                   
               
               
                 351 CAGCTCCCTG CCCCAGAGCT TCCTGCTCAA GTCTTTAGAG CAAGTGAGGA 
               
               
                   
               
               
                 401 AGATCCAGGG CGATGGCGCA GCGCTCCAGG AGAAGCTGTG TGCCACCTAC 
               
               
                   
               
               
                 451 AAGCTGTGCC ACCCCGAGGA GCTGGTGCTG CTCGGACACT CTCTGGGCAT 
               
               
                   
               
               
                 501 CCCCTGGGCT CCCCTGAGCT CCTGCCCCAG C 
               
               
                   
               
               
                 pG6968.Seq 
               
               
                   
               
               
                   1 CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA 
                 (SEQ ID NO:126) 
               
               
                   
               
               
                  51 CCAGGGGCTC CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA 
               
               
                   
               
               
                 101 CCTTGGACAC ACTGCAGCTG GACGTCGCCG ACTTTGCCAC CACCATCTGG 
               
               
                   
               
               
                 151 CAGCAGATGG AAGAACTGGG AATGGCCCCT GCCCTGCAGC CCACCCAGGG 
               
               
                   
               
               
                 201 TGCCATGCCG GCCTTCGCCT CTGCTTTCCA GCGCCGGGCA GGAGGGGTCC 
               
               
                   
               
               
                 251 TGGTTGCTAG CCATCTGCAG AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA 
               
               
                   
               
               
                 301 CGCCACCTTG CGCAGCCCGA CATGGCTACA CCATTAGGCC CTGCCAGCTC 
               
               
                   
               
               
                 351 CCTGCCCCAG AGCTTCCTGC TCAAGTCTTT AGAGCAAGTG AGGAAGATCC 
               
               
                   
               
               
                 401 AGGGCGATGG CGCAGCGCTC CAGGAGAAGC TGTGTGCCAC CTACAAGCTG 
               
               
                   
               
               
                 451 TGCCACCCCG AGGAGCTGGT GCTGCTCGGA CACTCTCTGG GCATCCCCTG 
               
               
                   
               
               
                 501 GGCTCCCCTG AGCTCCTGCC CCAGCCAGGC C 
               
               
                   
               
               
                 pG7170.Seq 
               
               
                   
               
               
                   1 CTGGCAGGCT GCTTGAGCCA ACTCCATAGC GGCCTTTTCC TCTACCAGGG 
                 (SEQ ID NO:127) 
               
               
                   
               
               
                  51 GCTCCTGCAG GCCCTGGAAG GGATATCCCC CGAGTTGGGT CCCACCTTGG 
               
               
                   
               
               
                 101 ACACACTGCA GCTGGACGTC GCCGACTTTG CCACCACCAT CTGGCAGCAG 
               
               
                   
               
               
                 151 ATGGAAGAAC TGGGAATGGC CCCTGCCCTG CAGCCCACCC AGGGTGCCAT 
               
               
                   
               
               
                 201 GCCGGCCTTC GCCTCTGCTT TCCAGCGCCG GGCAGGAGGG GTCCTGGTTG 
               
               
                   
               
               
                 251 CTAGCCATCT GCAGAGCTTC CTGGAGGTGT CGTACCGCGT TCTACGCCAC 
               
               
                   
               
               
                 301 CTTGCGCAGC CCGACATGGC TACACCATTA GGCCCTGCCA GCTCCCTGCC 
               
               
                   
               
               
                 351 CCAGAGCTTC CTGCTCAAGT CTTTAGAGCA AGTGAGGAAG ATCCAGGGCG 
               
               
                   
               
               
                 401 ATGGCGCAGC GCTCCAGGAG AAGCTGTGTG CCACCTACAA GCTGTGCCAC 
               
               
                   
               
               
                 451 CCCGAGGAGC TGGTGCTGCT CGGACACTCT CTGGGCATCC CCTGGGCTCC 
               
               
                   
               
               
                 501 CCTGAGCTCC TGCCCCAGCC AGGCCCTGCA G 
               
               
                   
               
               
                 pG170169-seq 
               
               
                   
               
               
                   1 CACCTTGCGC AGCCCGACAT GGCTACACCA TTAGGCCCTG CCAGCTCCCT 
                 (SEQ ID NO:129) 
               
               
                   
               
               
                  51 GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGG AAGATCCAGG 
               
               
                   
               
               
                 101 GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA CAAGCTGTGC 
               
               
                   
               
               
                 151 CACCCCGAGG AGCTGGTGCT GCTCGGACAC TCTCTGGGCA TCCCCTGGGC 
               
               
                   
               
               
                 201 TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA GGCTGCTTGA 
               
               
                   
               
               
                 251 GCCAACTCCA TAGCGGCCTT TTCCTCTACC AGGGGCTCCT GCAGGCCCTG 
               
               
                   
               
               
                 301 GAAGGGATAT CCCCCGAGTT GGGTCCCACC TTGGACACAC TGCAGCTGGA 
               
               
                   
               
               
                 351 CGTCGCCGAC TTTGCCACCA CCATCTGGCA GCAGATGGAA GAACTGGGAA 
               
               
                   
               
               
                 401 TGGCCCCTGC CCTGCAGCCC ACCCAGGGTG CCATGCCGGC CTTCGCCTCT 
               
               
                   
               
               
                 451 GCTTTCCAGC GCCGGGCAGG AGGGGTCCTG GTTGCTAGCC ATCTGCAGAG 
               
               
                   
               
               
                 501 CTTCCTGGAG GTGTCGTACC GCGTTCTACG C 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
               
             
           
               
                 TABLE 3 
               
               
                   
               
               
                 PROTEIN SEOUENCES 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 pMON3485.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:43) 
               
             
          
           
               
                 Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
               
               
                   
               
               
                 Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser 
               
               
                   
               
               
                 Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys 
               
               
                   
               
               
                 Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln 
               
               
                   
               
               
                 Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu 
               
               
                   
               
               
                 Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val 
               
               
                   
               
               
                 Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu 
               
               
                   
               
               
                 Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln 
               
               
                   
               
               
                 Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg 
               
               
                   
               
               
                 Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln 
               
               
                   
               
               
                 Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His 
               
               
                   
               
               
                 Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly Ser Gln 
               
               
                   
               
               
                 Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys 
               
               
                   
               
               
                 Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu 
               
               
                   
               
               
                 Cys Ala Thr 
               
               
                   
               
               
                   
               
               
                 pMON3486.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:44) 
               
             
          
           
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly 
               
               
                   
               
               
                 Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val 
               
               
                   
               
               
                 Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu 
               
               
                   
               
               
                 Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu 
               
               
                   
               
               
                 Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro 
               
               
                   
               
               
                 Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu 
               
               
                   
               
               
                 Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly 
               
               
                   
               
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser 
               
               
                   
               
               
                   
               
               
                 pMON3487.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:45) 
               
             
          
           
               
                 Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met 
               
               
                   
               
               
                 Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly 
               
               
                   
               
               
                 Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu 
               
               
                   
               
               
                 Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln 
               
               
                   
               
               
                 Pro Ser Gly Gly Ser Gly Gly Ser Gln Ser Phe Leu 
               
               
                   
               
               
                 Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly 
               
               
                   
               
               
                 Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr 
               
               
                   
               
               
                 Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
               
               
                   
               
               
                 Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser 
               
               
                   
               
               
                 Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys 
               
               
                   
               
               
                 Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln 
               
               
                   
               
               
                 Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu 
               
               
                   
               
               
                 Leu Gly Pro Thr Leu Asp Gln Met Glu Glu Leu Gly 
               
               
                   
               
               
                   
               
               
                 pMON3488.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:46) 
               
             
          
           
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly 
               
               
                   
               
               
                 Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val 
               
               
                   
               
               
                 Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu 
               
               
                   
               
               
                 Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu 
               
               
                   
               
               
                 Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro 
               
               
                   
               
               
                 Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu 
               
               
                   
               
               
                 Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly 
               
               
                   
               
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr 
               
               
                   
               
               
                 Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile 
               
               
                   
               
               
                 Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala 
               
               
                   
               
               
                 Leu Gln Pro 
               
               
                   
               
               
                   
               
               
                 pMON3489.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:47) 
               
             
          
           
               
                 Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val 
               
               
                   
               
               
                 Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr 
               
               
                   
               
               
                 Arg Val Leu Arg His Leu Ala Gln Pro Ser Gly Gly 
               
               
                   
               
               
                 Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr 
               
               
                   
               
               
                 Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
               
               
                   
               
               
                 Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met 
               
               
                   
               
               
                 Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro 
               
               
                   
               
               
                 Ala Phe Ala 
               
               
                   
               
               
                   
               
               
                 pMON3490.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:48) 
               
             
          
           
               
                 Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
               
               
                   
               
               
                 Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser 
               
               
                   
               
               
                 Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys 
               
               
                   
               
               
                 Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln 
               
               
                   
               
               
                 Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro G1u 
               
               
                   
               
               
                 Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val 
               
               
                   
               
               
                 Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu 
               
               
                   
               
               
                 Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln 
               
               
                   
               
               
                 Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg 
               
               
                   
               
               
                 Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln 
               
               
                   
               
               
                 Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His 
               
               
                   
               
               
                 Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser 
               
               
                   
               
               
                 Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln 
               
               
                   
               
               
                 Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln 
               
               
                   
               
               
                 Glu Lys Leu Cys Ala Thr 
               
               
                   
               
               
                   
               
               
                 pMON3491.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:49) 
               
             
          
           
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser 
               
               
                   
               
               
                   
               
               
                 pMON3492.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:50) 
               
             
          
           
               
                 Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met 
               
               
                   
               
               
                 Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly 
               
               
                   
               
               
                 Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu 
               
               
                   
               
               
                 Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln 
               
               
                   
               
               
                 Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln 
               
               
                   
               
               
                 Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys 
               
               
                   
               
               
                 Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu 
               
               
                   
               
               
                 Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
               
               
                   
               
               
                 Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala 
               
               
                   
               
               
                 Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu 
               
               
                   
               
               
                 Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe 
               
               
                   
               
               
                 Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
               
               
                   
               
               
                 Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln 
               
               
                   
               
               
                 Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln 
               
               
                   
               
               
                 Gln Met Glu Glu Leu Gly 
               
               
                   
               
               
                   
               
               
                 pMON3493.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:51) 
               
             
          
           
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln G1y Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr 
               
               
                   
               
               
                 Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
               
               
                   
               
               
                 Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met 
               
               
                   
               
               
                 Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                   
               
               
                 pMON3494.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:52) 
               
             
          
           
               
                 Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val 
               
               
                   
               
               
                 Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr 
               
               
                   
               
               
                 Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu 
               
               
                   
               
               
                 Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
               
               
                   
               
               
                 Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp 
               
               
                   
               
               
                 Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr 
               
               
                   
               
               
                 Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly 
               
               
                   
               
               
                 His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
               
               
                   
               
               
                 Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu 
               
               
                   
               
               
                 Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly 
               
               
                   
               
               
                 Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu 
               
               
                   
               
               
                 Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
               
               
                   
               
               
                 Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu 
               
               
                   
               
               
                 Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly 
               
               
                   
               
               
                 Ala Met Pro Ala Phe Ala 
               
               
                   
               
               
                   
               
               
                 pMON25181.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:53) 
               
             
          
           
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser 
               
               
                   
               
               
                   
               
               
                 pMON25182.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:54) 
               
             
          
           
               
                 Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met 
               
               
                   
               
               
                 Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly 
               
               
                   
               
               
                 Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu 
               
               
                   
               
               
                 Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln 
               
               
                   
               
               
                 Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln 
               
               
                   
               
               
                 Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys 
               
               
                   
               
               
                 Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu 
               
               
                   
               
               
                 Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
               
               
                   
               
               
                 Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala 
               
               
                   
               
               
                 Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu 
               
               
                   
               
               
                 Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe 
               
               
                   
               
               
                 Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
               
               
                   
               
               
                 Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln 
               
               
                   
               
               
                 Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln 
               
               
                   
               
               
                 Gln Met Glu Glu Leu Gly 
               
               
                   
               
               
                   
               
               
                 pMON25183.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:55) 
               
             
          
           
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr 
               
               
                   
               
               
                 Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
               
               
                   
               
               
                 Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met 
               
               
                   
               
               
                 Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                   
               
               
                 pMON25184.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:56) 
               
             
          
           
               
                 Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val 
               
               
                   
               
               
                 Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr 
               
               
                   
               
               
                 Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu 
               
               
                   
               
               
                 Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
               
               
                   
               
               
                 Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp 
               
               
                   
               
               
                 Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr 
               
               
                   
               
               
                 Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly 
               
               
                   
               
               
                 His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
               
               
                   
               
               
                 Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu 
               
               
                   
               
               
                 Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly 
               
               
                   
               
               
                 Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu 
               
               
                   
               
               
                 Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
               
               
                   
               
               
                 Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu 
               
               
                   
               
               
                 Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly 
               
               
                   
               
               
                 Ala Met Pro Ala Phe Ala 
               
               
                   
               
               
                   
               
               
                 pMON25185.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:57) 
               
             
          
           
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly 
               
               
                   
               
               
                 Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val 
               
               
                   
               
               
                 Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu 
               
               
                   
               
               
                 Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu 
               
               
                   
               
               
                 Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro 
               
               
                   
               
               
                 Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu 
               
               
                   
               
               
                 Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly 
               
               
                   
               
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser 
               
               
                   
               
               
                   
               
               
                 pMON25186.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:58) 
               
             
          
           
               
                 Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met 
               
               
                   
               
               
                 Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly 
               
               
                   
               
               
                 Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu 
               
               
                   
               
               
                 Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln 
               
               
                   
               
               
                 Pro Ser Gly Gly Ser Gly Gly Ser Gln Ser Phe Leu 
               
               
                   
               
               
                 Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly 
               
               
                   
               
               
                 Asp Gly Ala Ala Leu Gln Glu Lys Ala Thr Tyr Lys 
               
               
                   
               
               
                 Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His 
               
               
                   
               
               
                 Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys 
               
               
                   
               
               
                 Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser 
               
               
                   
               
               
                 Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu 
               
               
                   
               
               
                 Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly 
               
               
                   
               
               
                 Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp 
               
               
                   
               
               
                 Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu 
               
               
                   
               
               
                 Gly 
               
               
                   
               
               
                   
               
               
                 pMON25187.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:59) 
               
             
          
           
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly 
               
               
                   
               
               
                 Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val 
               
               
                   
               
               
                 Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu 
               
               
                   
               
               
                 Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu 
               
               
                   
               
               
                 Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro 
               
               
                   
               
               
                 Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu 
               
               
                   
               
               
                 Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly 
               
               
                   
               
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr 
               
               
                   
               
               
                 Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile 
               
               
                   
               
               
                 Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala 
               
               
                   
               
               
                 Leu Gln Pro 
               
               
                   
               
               
                   
               
               
                 pMON25188.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:60) 
               
             
          
           
               
                 Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val 
               
               
                   
               
               
                 Ala Ser His Leu Gln Ser Phe Leu Giu Val Ser Tyr 
               
               
                   
               
               
                 Arg Val Leu Arg His Leu Ala Gln Pro Ser Gly Gly 
               
               
                   
               
               
                 Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr 
               
               
                   
               
               
                 Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
               
               
                   
               
               
                 Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met 
               
               
                   
               
               
                 Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro 
               
               
                   
               
               
                 Ala Phe Ala 
               
               
                   
               
               
                   
               
               
                 pMON3460.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:95) 
               
             
          
           
               
                 Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro 
               
               
                   
               
               
                 Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala 
               
               
                   
               
               
                 Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu 
               
               
                   
               
               
                 Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser 
               
               
                   
               
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Giy Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val 
               
               
                   
               
               
                   
               
               
                 pMON3461.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:96) 
               
             
          
           
               
                 Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu 
               
               
                   
               
               
                 Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly 
               
               
                   
               
               
                 Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp 
               
               
                   
               
               
                 Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu 
               
               
                   
               
               
                 Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala 
               
               
                   
               
               
                 Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala 
               
               
                   
               
               
                 Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe 
               
               
                   
               
               
                 Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala 
               
               
                   
               
               
                 Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro 
               
               
                   
               
               
                 Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg 
               
               
                   
               
               
                 Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys 
               
               
                   
               
               
                 Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu 
               
               
                   
               
               
                 Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp 
               
               
                   
               
               
                 Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln 
               
               
                   
               
               
                 Leu Ala Gly Cys Leu Ser 
               
               
                   
               
               
                   
               
               
                 3462.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:97) 
               
             
          
           
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr 
               
               
                   
               
               
                 Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile 
               
               
                   
               
               
                 Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala 
               
               
                   
               
               
                 Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala 
               
               
                   
               
               
                 Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val 
               
               
                   
               
               
                 Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr 
               
               
                   
               
               
                 Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu 
               
               
                   
               
               
                 Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
               
               
                   
               
               
                 Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp 
               
               
                   
               
               
                 Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr 
               
               
                   
               
               
                 Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly 
               
               
                   
               
               
                 His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
               
               
                   
               
               
                 Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu 
               
               
                   
               
               
                 Ser Gln Leu His Ser G1y 
               
               
                   
               
               
                   
               
               
                 3463.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:98) 
               
             
          
           
               
                 Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
               
               
                   
               
               
                 Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln 
               
               
                   
               
               
                 Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln 
               
               
                   
               
               
                 Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln 
               
               
                   
               
               
                 Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
               
               
                   
               
               
                 Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser 
               
               
                   
               
               
                 His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val 
               
               
                   
               
               
                 Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro 
               
               
                   
               
               
                 Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser 
               
               
                   
               
               
                 Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala 
               
               
                   
               
               
                 Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu 
               
               
                   
               
               
                 Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser 
               
               
                   
               
               
                 Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro 
               
               
                   
               
               
                 Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln 
               
               
                   
               
               
                 Leu His Ser Gly Leu Phe 
               
               
                   
               
               
                   
               
               
                 3464.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:99) 
               
             
          
           
               
                 Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr 
               
               
                   
               
               
                 Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
               
               
                   
               
               
                 Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met 
               
               
                   
               
               
                 Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro 
               
               
                   
               
               
                 Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly 
               
               
                   
               
               
                 Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu 
               
               
                   
               
               
                 Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
               
               
                   
               
               
                 Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser 
               
               
                   
               
               
                 Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile 
               
               
                   
               
               
                 Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys 
               
               
                   
               
               
                 Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val 
               
               
                   
               
               
                 Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro 
               
               
                   
               
               
                 Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala 
               
               
                   
               
               
                 Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu 
               
               
                   
               
               
                 Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                   
               
               
                 3465.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:100) 
               
             
          
           
               
                 Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu 
               
               
                   
               
               
                 Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly 
               
               
                   
               
               
                 Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg 
               
               
                   
               
               
                 Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
               
               
                   
               
               
                 Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu 
               
               
                   
               
               
                 Ala Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu 
               
               
                   
               
               
                 Pro Gln Ser Phe Leu Leu Lys Ser Leu Giu Gln Val 
               
               
                   
               
               
                 Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu 
               
               
                   
               
               
                 Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu 
               
               
                   
               
               
                 Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro 
               
               
                   
               
               
                 Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu 
               
               
                   
               
               
                 Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly 
               
               
                   
               
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr 
               
               
                   
               
               
                 Leu Gln Leu Asp Val Ala 
               
               
                   
               
               
                   
               
               
                 3466.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:101) 
               
             
          
           
               
                 Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro 
               
               
                   
               
               
                 Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe 
               
               
                   
               
               
                 Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu 
               
               
                   
               
               
                 Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser 
               
               
                   
               
               
                 Tyr Arg Val Leu Arg His Leu Ala Gin Pro Thr Pro 
               
               
                   
               
               
                 Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu 
               
               
                   
               
               
                 Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly 
               
               
                   
               
               
                 Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr 
               
               
                   
               
               
                 Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
               
               
                   
               
               
                 Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser 
               
               
                   
               
               
                 Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys 
               
               
                   
               
               
                 Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln 
               
               
                   
               
               
                 Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu 
               
               
                   
               
               
                 Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val 
               
               
                   
               
               
                 Ala Asp Phe Ala Thr Thr 
               
               
                   
               
               
                   
               
               
                 3467.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:102) 
               
             
          
           
               
                 Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu 
               
               
                   
               
               
                 Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser 
               
               
                   
               
               
                 Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala 
               
               
                   
               
               
                 Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg 
               
               
                   
               
               
                 Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly 
               
               
                   
               
               
                 Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys 
               
               
                   
               
               
                 Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly 
               
               
                   
               
               
                 Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys 
               
               
                   
               
               
                 Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His 
               
               
                   
               
               
                 Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys 
               
               
                   
               
               
                 Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser 
               
               
                   
               
               
                 Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu 
               
               
                   
               
               
                 Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly 
               
               
                   
               
               
                 Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp 
               
               
                   
               
               
                 Phe Ala Thr Thr Ile Trp 
               
               
                   
               
               
                   
               
               
                 3499.Pep 
               
             
          
           
               
                   
                 (SEQ ID NO:103) 
               
             
          
           
               
                 Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro 
               
               
                   
               
               
                 Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala 
               
               
                   
               
               
                 Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu 
               
               
                   
               
               
                 Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser 
               
               
                   
               
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val 
               
               
                   
               
               
                   
               
               
                 pG1110.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:104) 
               
             
          
           
               
                 GlnSerPheLeuLeuLysSerLeuGluGlnValArgLysIleGlnGly 
               
               
                   
               
               
                 AspGlyAlaAlaLeuGlnGluLysLeuCysAlaThrTyrLysLeuCys 
               
               
                   
               
               
                 HisProGluGluLeuValLeuLeuGlyHisSerLeuGlyIleProTrp 
               
               
                   
               
               
                 AlaProLeuSerSerCysProSerGlnAlaLeuGlnLeuAlaGlyCys 
               
               
                   
               
               
                 LeuSerGlnLeuHisSerGlyLeuPheLeuTyrGlnGlyLeuLeuGln 
               
               
                   
               
               
                 AlaLeuGluGlyIleSerProGluLeuGlyProThrLeuAspThrLeu 
               
               
                   
               
               
                 GlnLeuAspValAlaAspPheAlaThrThrIleTrpGlnGlnMetGlu 
               
               
                   
               
               
                 GluLeuGlyMetAlaProAlaLeuGlnProThrGlnGlyAlaMetPro 
               
               
                   
               
               
                 AlaPheAlaSerAlaPheGlnArgArgAlaGlyGlyValLeuValAla 
               
               
                   
               
               
                 SerHisLeuGlnSerPheLeuGluValSerTyrArgValLeuArgHis 
               
               
                   
               
               
                 LeuAlaGlnProAspMetAlaThrProLeuGlyProAlaSerSerLeu 
               
               
                   
               
               
                 Pro 
               
               
                   
               
               
                   
               
               
                 pG123122.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:105) 
               
             
          
           
               
                 GluLeuGlyMetAlaProAlaLeuGlnProThrGlnGlyAlaMetPro 
               
               
                   
               
               
                 AlaPheAlaSerAlaPheGlnArgArgAlaGlyGlyValLeuValAla 
               
               
                   
               
               
                 SerHisLeuGlnSerPheLeuGluValSerTyrArgValLeuArgHis 
               
               
                   
               
               
                 LeuAlaGlnProAspMetAlaThrProLeuGlyProAlaSerSerLeu 
               
               
                   
               
               
                 ProGlnSerPheLeuLeuLysSerLeuGluGlnValArgLysIleGln 
               
               
                   
               
               
                 GlyAspGlyAlaAlaLeuGlnGluLysLeuCysAlaThrTyrLysLeu 
               
               
                   
               
               
                 CysHisProGluGluLeuValLeuLeuGlyHisSerLeuGlyIlePro 
               
               
                   
               
               
                 TrpAlaProLeuSerSerCysProSerGlnAlaLeuGlnLeuAlaGly 
               
               
                   
               
               
                 CysLeuSerGlnLeuHisSerGlyLeuPheLeuTyrGlnGlyLeuLeu 
               
               
                   
               
               
                 GlnAlaLeuGluGlyIleSerProGluLeuGlyProThrLeuAspThr 
               
               
                   
               
               
                 LeuGlnLeuAspValAlaAspPheAlaThrThrIleTrpGlnGlnMet 
               
               
                   
               
               
                 Glu 
               
               
                   
               
               
                   
               
               
                 pG125124.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:106) 
               
             
          
           
               
                 GlyMetAlaProAlaLeuGlnProThrGlnGlyAlaMetProAlaPhe 
               
               
                   
               
               
                 AlaSerAlaPheGlnArgArgAlaGlyGlyValLeuValAlaSerHis 
               
               
                   
               
               
                 LeuGlnSerPheLeuGluValSerTyrArgValLeuArgHisLeuAla 
               
               
                   
               
               
                 GlnProAspMetAlaThrProLeuGlyProAlaSerSerLeuProGln 
               
               
                   
               
               
                 SerPheLeuLeuLysSerLeuGluGlnValArgLysIleGlnGlyAsp 
               
               
                   
               
               
                 GlyAlaAlaLeuGlnGluLysLeuCysAlaThrTyrLysLeuCysHis 
               
               
                   
               
               
                 ProGluGluLeuValLeuLeuGlyHisSerLeuGlyIleProTrpAla 
               
               
                   
               
               
                 ProLeuSerSerCysProSerGlnAlaLeuGlnLeuAlaGlyCysLeu 
               
               
                   
               
               
                 SerGlnLeuHisSerGlyLeuPheLeuTyrGlnGlyLeuLeuGlnAla 
               
               
                   
               
               
                 LeuGluGlyIleSerProGluLeuGlyProThrLeuAspThrLeuGln 
               
               
                   
               
               
                 LeuAspValAlaAspPheAlaThrThrIleTrpGlnGlnMetGluGlu 
               
               
                   
               
               
                 Leu 
               
               
                   
               
               
                   
               
               
                 pG1312.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:107) 
               
             
          
           
               
                 PheLeuLeuLysSerLeuGluGlnValArgLysIleGlnGlyAspGly 
               
               
                   
               
               
                 AlaAlaLeuGlnGluLysLeuCysAlaThrTyrLysLeuCysHisPro 
               
               
                   
               
               
                 GluGluLeuValLeuLeuGlyHisSerLeuGlyIleProTrpAlaPro 
               
               
                   
               
               
                 LeuSerSerCysProSerGlnAlaLeuGlnLeuAlaGlyCysLeuSer 
               
               
                   
               
               
                 GlnLeuHisSerGlyLeuPheLeuTyrGlnGlyLeuLeuGlnAlaLeu 
               
               
                   
               
               
                 GluGlyIleSerProGluLeuGlyProThrLeuAspThrLeuGlnLeu 
               
               
                   
               
               
                 AspValAlaAspPheAlaThrThrIleTrpGlnGlnMetGluGluLeu 
               
               
                   
               
               
                 GlyMetAlaProAlaLeuGlnProThrGlnGlyAlaMetProAlaPhe 
               
               
                   
               
               
                 AlaSerAlaPheGlnArgArgAlaGlyGlyValLeuValAlaSerHis 
               
               
                   
               
               
                 LeuGlnSerPheLeuGluValSerTyrArgValLeuArgHisLeuAla 
               
               
                   
               
               
                 GlnProAspMetAlaThrProLeuGlyProAlaSerSerLeuProGln 
               
               
                   
               
               
                 Ser 
               
               
                   
               
               
                   
               
               
                 pG159158.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:108) 
               
             
          
           
               
                 SerPheLeuGluValSerTyrArgValLeuArgHisLeuAlaGlnPro 
               
               
                   
               
               
                 AspMetAlaThrProLeuGlyProAlaSerSerLeuProGlnSerPhe 
               
               
                   
               
               
                 LeuLeuLysSerLeuGluGlnValArgLysIleGlnGlyAspGlyAla 
               
               
                   
               
               
                 AlaLeuGlnGluLysLeuCysAlaThrTyrLysLeuCysHisProGlu 
               
               
                   
               
               
                 GluLeuValLeuLeuGlyHisSerLeuGlyIleProTrpAlaProLeu 
               
               
                   
               
               
                 SerSerCysProSerGlnAlaLeuGlnLeuAlaGlyCysLeuSerGln 
               
               
                   
               
               
                 LeuHisSerGlyLeuPheLeuTyrGlnGlyLeuLeuGlnAlaLeuGlu 
               
               
                   
               
               
                 GlyIleSerProGluLeuGlyProThrLeuAspThrLeuGlnLeuAsp 
               
               
                   
               
               
                 ValAlaAspPheAlaThrThrIleTrpGlnGlnMetGluGluLeuGly 
               
               
                   
               
               
                 MetAlaProAlaLeuGlnProThrGlnGlyAlaMetProAlaPheAla 
               
               
                   
               
               
                 SerAlaPheGlnArgArgAlaGlyGlyValLeuValAlaSerHisLeu 
               
               
                   
               
               
                 Gln 
               
               
                   
               
               
                   
               
               
                 pG1918.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:109) 
               
             
          
           
               
                 GluGlnValArgLysIleGlnGlyAspGlyAlaAlaLeuGlnGluLys 
               
               
                   
               
               
                 LeuCysAlaThrTyrLysLeuCysHisProGluGluLeuValLeuLeu 
               
               
                   
               
               
                 GlyHisSerLeuGlyIleProTrpAlaProLeuSerSerCysProSer 
               
               
                   
               
               
                 GlnAlaLeuGlnLeuAlaGlyCysLeuSerGlnLeuHisSerGlyLeu 
               
               
                   
               
               
                 PheLeuTyrGlnGlyLeuLeuGlnAlaLeuGluGlyIleSerProGlu 
               
               
                   
               
               
                 LeuGlyProThrLeuAspThrLeuGlnLeuAspValAlaAspPheAla 
               
               
                   
               
               
                 ThrThrIleTrpGlnGlnMetGluGluLeuGlyMetAlaProAlaLeu 
               
               
                   
               
               
                 GlnProThrGlnGlyAlaMetProAlaPheAlaSerAlaPheGlnArg 
               
               
                   
               
               
                 ArgAlaGlyGlyValLeuValAlaSerHisLeuGlnSerPheLeuGlu 
               
               
                   
               
               
                 ValSerTyrArgValLeuArgHisLeuAlaGlnProAspMetAlaThr 
               
               
                   
               
               
                 ProLeuGlyProAlaSerSerLeuProGlnSerPheLeuLeuLysSer 
               
               
                   
               
               
                 Leu 
               
               
                   
               
               
                   
               
               
                 pG32.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:110) 
               
             
          
           
               
                 LeuGlyProAlaSerSerLeuProGlnSerPheLeuLeuLysSerLeu 
               
               
                   
               
               
                 GluGlnValArgLysIleGlnGlyAspGlyAlaAlaLeuGlnGluLys 
               
               
                   
               
               
                 LeuCysAlaThrTyrLysLeuCysHisProGluGluLeuValLeuLeu 
               
               
                   
               
               
                 GlyHisSerLeuGlyIleProTrpAlaProLeuSerSerCysProSer 
               
               
                   
               
               
                 GlnAlaLeuGlnLeuAlaGlyCysLeuSerGlnLeuHisSerGlyLeu 
               
               
                   
               
               
                 PheLeuTyrGlnGlyLeuLeuGlnAlaLeuGluGlyIleSerProGlu 
               
               
                   
               
               
                 LeuGlyProThrLeuAspThrLeuGlnLeuAspValAlaAspPheAla 
               
               
                   
               
               
                 ThrThrIleTrpGlnGlnMetGluGluLeuGlyMetAlaProAlaLeu 
               
               
                   
               
               
                 GlnProThrGlnGlyAlaMetProAlaPheAlaSerAlaPheGlnArg 
               
               
                   
               
               
                 ArgALaGlyGlyValLeuValAlaSerHisLeuGlnSerPheLeuGlu 
               
               
                   
               
               
                 ValSerTyrArgValLeuArgHisLeuAlaGlnProAspMetAlaThr 
               
               
                   
               
               
                 Pro 
               
               
                   
               
               
                   
               
               
                 pG4948.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:111) 
               
             
          
           
               
                 LeuLeuGlyHisSerLeuGlyIleProTrpAlaProLeuSerSerCys 
               
               
                   
               
               
                 ProSerGlnAlaLeuGlnLeuAlaGlyCysLeuSerGlnLeuHisSer 
               
               
                   
               
               
                 GlyLeuPheLeuTyrGlnGlyLeuLeuGlnAlaLeuGluGlyIleSer 
               
               
                   
               
               
                 ProGluLeuGlyProThrLeuAspThrLeuGlnLeuAspValAlaAsp 
               
               
                   
               
               
                 PheAlaThrThrIleTrpGlnGlnMetGluGluLeuGlyMetAlaPro 
               
               
                   
               
               
                 AlaLeuGlnProThrGlnGlyAlaMetProAlaPheAlaSerAlaPhe 
               
               
                   
               
               
                 GlnArgArgAlaGlyGlyValLeuValAlaSerHisLeuGlnSerPhe 
               
               
                   
               
               
                 LeuGluValSerTyrArgValLeuArgHisLeuAlaGlnProAspMet 
               
               
                   
               
               
                 AlaThrProLeuGlyProAlaSerSerLeuProGlnSerPheLeuLeu 
               
               
                   
               
               
                 LysSerLeuGluGlnValArgLysIleGlnGlyAspGlyAlaAlaLeu 
               
               
                   
               
               
                 GlnGluLysLeuCysAlaThrTyrLysLeuCysHiSProGluGluLeu 
               
               
                   
               
               
                 Val 
               
               
                   
               
               
                   
               
               
                 pG6059.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:112) 
               
             
          
           
               
                 ProLeuSerSerCysProSerGlnAlaLeuGlnLeuAlaGlyCysLeu 
               
               
                   
               
               
                 SerGlnLeuHisSerGlyLeuPheLeuTyrGlnGlyLeuLeuGlnAla 
               
               
                   
               
               
                 LeuGluGlyIleSerProGluLeuGlyProThrLeuAspThrLeuGln 
               
               
                   
               
               
                 LeuAspValAlaAspPheAlaThrThrIleTrpGlnGlnMetGluGlu 
               
               
                   
               
               
                 LeuGlyMetAlaProAlaLeuGlnProThrGlnGlyAlaMetProAla 
               
               
                   
               
               
                 PheAlaSerAlaPheGlnArgArgAlaGlyGlyValLeuValAlaSer 
               
               
                   
               
               
                 HisLeuGlnSerPheLeuGluValSerTyrArgValLeuArgHisLeu 
               
               
                   
               
               
                 AlaGlnProAspMetAlaThrProLeuGlyProAlaSerSerLeuPro 
               
               
                   
               
               
                 GlnSerPheLeuLeuLysSerLeuGluGlnValArgLysIleGlnGly 
               
               
                   
               
               
                 AspGlyAlaAlaLeuGlnGluLysLeuCysAlaThrTyrLysLeuCys 
               
               
                   
               
               
                 HisProGluGluLeuValLeuLeuGlyHisSerLeuGlyIleProTrp 
               
               
                   
               
               
                 Ala 
               
               
                   
               
               
                   
               
               
                 pG6766.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:113) 
               
             
          
           
               
                 GlnAlaLeuGlnLeuAlaGlyCysLeuSerGlnLeuHisSerGlyLeu 
               
               
                   
               
               
                 PheLeuTyrGlnGlyLeuLeuGlnAlaLeuGluGlyIleSerProGlu 
               
               
                   
               
               
                 LeuGlyProThrLeuAspThrLeuGlnLeuAspValAlaAspPheAla 
               
               
                   
               
               
                 ThrThrIleTrpGlnGlnMetGluGluLeuGlyMetAlaProAlaLeu 
               
               
                   
               
               
                 GlnProThrGlnGlyAlaMetProAlaPheAlaSerAlaPheGlnArg 
               
               
                   
               
               
                 ArgAlaGlyGlyValLeuValAlaSerHisLeuGlnSerPheLeuGlu 
               
               
                   
               
               
                 ValSerTyrArgValLeuArgHisLeuAlaGlnProAspMetAlaThr 
               
               
                   
               
               
                 ProLeuGlyProAlaSerSerLeuProGlnSerPheLeuLeuLysSer 
               
               
                   
               
               
                 LeuGluGlnValArgLysIleGlnGlyAspGlyAlaAlaLeuGlnGlu 
               
               
                   
               
               
                 LysLeuCysAlaThrTyrLysLeuCysHisProGluGluLeuValLeu 
               
               
                   
               
               
                 LeuGlyHisSerLeuGlyIleProTrpAlaProLeuSerSerCysPro 
               
               
                   
               
               
                 Ser 
               
               
                   
               
               
                   
               
               
                 pG6968.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:114) 
               
             
          
           
               
                 LeuGlnLeuAlaGlyCysLeuSerGlnLeuHisSerGlyLeuPheLeu 
               
               
                   
               
               
                 TyrGlnGlyLeuLeuGlnAlaLeuGluGlyIleSerProGluLeuGly 
               
               
                   
               
               
                 ProThrLeuAspThrLeuGlnLeuAspValAlaAspPheAlaThrThr 
               
               
                   
               
               
                 IleTrpGlnGlnMetGluGluLeuGlyMetAlaProAlaLeuGlnPro 
               
               
                   
               
               
                 ThrGlnGlyAlaMetProAlaPheAlaSerAlaPheGlnArgArgAla 
               
               
                   
               
               
                 GlyGlyValLeuValAlaSerHisLeuGlnSerPheLeuGluValSer 
               
               
                   
               
               
                 TyrArgValLeuArgHisLeuAlaGlnProAspMetAlaThrProLeu 
               
               
                   
               
               
                 GlyProAlaSerSerLeuProGlnSerPheLeuLeuLysSerLeuGlu 
               
               
                   
               
               
                 GlnValArgLysIleGlnGlyAspGlyAlaAlaLeuGlnGluLysLeu 
               
               
                   
               
               
                 CysAlaThrTyrLysLeuCysHisProGluGluLeuValLeuLeuGly 
               
               
                   
               
               
                 HisSerLeuGlyIleProTrpAlaProLeuSerSerCysProSerGln 
               
               
                   
               
               
                 Ala 
               
               
                   
               
               
                   
               
               
                 pG7170.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:115) 
               
             
          
           
               
                 LeuAlaGlyCysLeuSerGlnLeuHisSerGlyLeuPheLeuTyrGln 
               
               
                   
               
               
                 GlyLeuLeuGlnAlaLeuGluGlyIleSerProGluLeuGlyProThr 
               
               
                   
               
               
                 LeuAspThrLeuGlnLeuAspValAlaAspPheAlaThrThrIleTrp 
               
               
                   
               
               
                 GlnGlnMetGluGluLeuGlyMetAlaProAlaLeuGlnProThrGln 
               
               
                   
               
               
                 GlyAlaMetProAlaPheAlaSerAlaPheGlnArgArgAlaGlyGly 
               
               
                   
               
               
                 ValLeuValAlaSerHisLeuGlnSerPheLeuGluValSerTyrArg 
               
               
                   
               
               
                 ValLeuArgHisLeuAlaGlnProAspMetAlaThrProLeuGlyPro 
               
               
                   
               
               
                 AlaSerSerLeuProGlnSerPheLeuLeuLysSerLeuGluGlnVal 
               
               
                   
               
               
                 ArgLysIleGlnGlyAspGlyAlaAlaLeuGlnGluLysLeuCysAla 
               
               
                   
               
               
                 ThrTyrLysLeuCysHisProGluGluLeuValLeuLeuGlyHisSer 
               
               
                   
               
               
                 LeuGlyIleProTrpAlaProLeuSerSerCysProSerGlnAlaLeu 
               
               
                   
               
               
                 Gln 
               
               
                   
               
               
                   
               
               
                 p170169.pep 
               
             
          
           
               
                   
                 (SEQ ID NO:128) 
               
             
          
           
               
                 1 
                 His Leu Ala Gln Pro Asp Met Ala Thr Pro Leu Gly 
               
               
                   
                 Pro Ala Ser  
               
               
                   
               
               
                 16 
                 Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu 
               
               
                   
                 Gln Val Arg 
               
               
                   
               
               
                 31 
                 Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys 
               
               
                   
                 Leu Cys Ala 
               
               
                   
               
               
                 46 
                 Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu 
               
               
                   
                 Leu Gly His 
               
               
                   
               
               
                 61 
                 Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys 
               
               
                   
                 Pro Ser Gln 
               
               
                   
               
               
                 76 
                 Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
               
               
                   
                 Ser Gly Leu 
               
               
                   
               
               
                 91 
                 Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly 
               
               
                   
                 Ile Ser Pro 
               
               
                   
               
               
                 106 
                 Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp 
               
               
                   
                 Val Ala Asp 
               
               
                   
               
               
                 121 
                 Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu 
               
               
                   
                 Gly Met Ala 
               
               
                   
               
               
                 136 
                 Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala 
               
               
                   
                 Phe Ala Ser 
               
               
                   
               
               
                 151 
                 Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala 
               
               
                   
                 Ser His Leu 
               
               
                   
               
               
                 155 
                 Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg 
               
             
          
         
       
     
     Materials and Methods 
     Recombinant DNA Methods 
     Unless noted otherwise, all specialty chemicals were obtained from Sigma Co., (St. Louis, Mo.). Restriction endonucleases and T4 DNA ligase were obtained from New England Biolabs (Beverly, Mass.) or Boehringer Mannheim (Indianapolis, Ind.). 
     Transformation of  E. coli  Strains 
       E. coli  strains, such as DH5α™ (Life Technologies, Gaithersburg, Md.) and TG1 (Amersham Corp., Arlington Heights, Ill.) are used for transformation of ligation reactions and are the source of plasmid DNA for transfecting mammalian cells.  E. coli  strains, such as MON105 and JM101, can be used for expressing the G-CSF receptor agonist of the present invention in the cytoplasm or periplasmic space. 
     MON105 ATCC#55204: F-, lamda-,IN(rrnD, rrE)1, rpoD+, rpoH358 
     DH5α™: F-, phi80dlacZdeltaM15, delta(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk−,mk+), phoA, supE44lamda-, thi-1, gyrA96, relA1 
     TG1: delta(lac-pro), supE, thi-1, hsdD5/F′ (traD36, proA+B+, lacIq, lacZdeltaM15) 
     DH5α™ Subcloning efficiency cells are purchased as competent cells and are ready for transformation using the manufacturer&#39;s protocol, while both  E. coli  strains TG1 and MON105 are rendered competent to take up DNA using a CaCl 2  method. Typically, 20 to 50 mL of cells are grown in LB medium (1% Bacto-tryptone, 0.5% Bacto-yeast extract, 150 mM NaCl) to a density of approximately 1.0 optical density unit at 600 nanometers (OD600) as measured by a Baush &amp; Lomb Spectronic spectrophotometer (Rochester, N.Y.). The cells are collected by centrifugation and resuspended in one-fifth culture volume of CaCl 2  solution (50 mM CaCl 2 , 10 mM Tris-Cl, pH7.4) and are held at 4° C. for 30 minutes. The cells are again collected by centrifugation and resuspended in one-tenth culture volume of CaCl 2  solution. Ligated DNA is added to 0.2 mL of these cells, and the samples are held at 4° C. for 1 hour. The samples are shifted to 42° C. for two minutes and 1 mL of LB is added prior to shaking the samples at 37° C. for one hour. Cells from these samples are spread on plates (LB medium plus 1.5% Bacto-agar) containing either ampicillin (100 micrograms/mL, ug/mL) when selecting for ampicillin-resistant transformants, or spectinomycin (75 ug/mL) when selecting for spectinomycin-resistant transformants. The plates are incubated overnight at 37° C. Single colonies are picked, grown in LB supplemented with appropriate antibiotic for 6-16 hours at 37° C. with shaking. Colonies are picked and inoculated into LB plus appropriate antibiotic (100 ug/mL ampicillin or 75 ug/mL spectinomycin) and are grown at 37° C. while shaking. Before harvesting the cultures, 1 ul of cells are analyzed by PCR for the presence of a G-CSF gene. The PCR is carried out using a combination of primers that anneal to the G-CSF gene and/or vector. After the PCR is complete, loading dye is added to the sample followed by electrophoresis as described earlier. A gene has been ligated to the vector when a PCR product of the expected size is observed. 
     Methods for Creation of Genes with New N-terminus/C-terminus 
     Method I. Creation of genes with new N-terminus/C-terminus which contain a linker region. 
     Genes with new N-terminus/C-terminus which contain a linker region separating the original C-terminus and N-terminus can be made essentially following the method described in L. S. Mullins, et al  J. Am. Chem. Soc.  116, 5529-5533 (1994). Multiple steps of polymerase chain reaction (PCR) amplifications are used to rearrange the DNA sequence encoding the primary amino acid sequence of the protein. The steps are illustrated in FIG.  2 . 
     In the first step, the primer set (“new start” and “linker start”) is used to create and amplify, from the original gene sequence, the DNA fragment (“Fragment Start”) that contains the sequence encoding the new N-terminal portion of the new protein followed by the linker that connects the C-terminal and N-terminal ends of the original protein. In the second step, the primer set (“new stop” and “linker stop”) is used to create and amplify, from the original gene sequence, the DNA fragment (“Fragment Stop”) that encodes the same linker as used above, followed by the new C-terminal portion of the new protein. The “new start” and “new stop” primers are designed to include the appropriate restriction enzyme recognition sites which allow cloning of the new gene into expression plasmids. Typical PCR conditions are one cycle 95° C. melting for two minutes; 25 cycles 94° C. denaturation for one minute, 50° C. annealing for one minute and 72° C. extension for one minute; plus one cycle 72° C. extension for seven minutes. A Perkin Elmer GeneAmp PCR Core Reagents kit is used. A 100 ul reaction contains 100 pmole of each primer and one ug of template DNA; and 1×PCR buffer, 200 uM dGTP, 200 uM DATP, 200 uM dTTP, 200 uM dCTP, 2.5 units AmpliTaq DNA polymerase and 2 mM MgCl 2 . PCR reactions are performed in a Model 480 DNA thermal cycler (Perkin Elmer Corporation, Norwalk, Conn.). 
     “Fragment Start” and “Fragment Stop”, which have complementary sequence in the linker region and the coding sequence for the two amino acids on both sides of the linker, are joined together in a third PCR step to make the full-length gene encoding the new protein. The DNA fragments “Fragment Start” and “Fragment Stop” are resolved on a 1% TAE gel, stained with ethidium bromide and isolated using a Qiaex Gel Extraction kit (Qiagen). These fragments are combined in equimolar quantities, heated at 70° C. for ten minutes and slow cooled to allow annealing through their shared sequence in “linker start” and “linker stop”. In the third PCR step, primers “new start” and “new stop” are added to the annealed fragments to create and amplify the full-length new N-terminus/C-terminus gene. Typical PCR conditions are one cycle 95° C. melting for two minutes; 25 cycles 94° C. denaturation for one minute, 60° C. annealing for one minute and 72° C. extension for one minute; plus one cycle 72° C. extension for seven minutes. A Perkin Elmer GeneAmp PCR Core Reagents kit is used. A 100 ul reaction contains 100 pmole of each primer and approximately 0.5 ug of DNA; and 1×PCR buffer, 200 uM dGTP, 200 uM dATP, 200 uM dTTP, 200 uM dCTP, 2.5 units AmpliTaq DNA polymerase and 2 mM MgCl 2 . PCR reactions are purified using a Wizard PCR Preps kit (Promega). 
     Method II. Creation of genes with new N-terminus/C-terminus without a linker region. 
     New N-terminus/C-terminus genes without a linker joining the original N-terminus and C-terminus can be made using two steps of PCR amplification and a blunt end ligation. The steps are illustrated in FIG.  3 . In the first step, the primer set (“new start” and “P-bl start”) is used to create and amplify, from the original gene sequence, the DNA fragment (“Fragment Start”) that contains the sequence encoding the new N-terminal portion of the new protein. In the second step, the primer set (“new stop” and “P-bl stop”) is used to create and amplify, from the original gene sequence, the DNA fragment (“Fragment Stop”) that contains the sequence encoding the new C-terminal portion of the new protein. The “new start” and “new stop” primers are designed to include appropriate restriction sites which allow cloning of the new gene into expression vectors. Typical PCR conditions are one cycle 95° C. melting for two minutes; 25 cycles 94° C. denaturation for one minute, 50° C. annealing for 45 seconds and 72° C. extension for 45 seconds. Deep Vent polymerase (New England Biolabs) is used to reduce the occurrence of overhangs in conditions recommended by the manufacturer. The “P-bl start” and “P-bl stop” primers are phosphorylated at the 5′ end to aid in the subsequent blunt end ligation of “Fragment Start” and “Fragment Stop” to each other. A 100 ul reaction contained 150 pmole of each primer and one ug of template DNA; and 1×Vent buffer (New England Biolabs), 300 uM dGTP, 300 uM DATP, 300 uM dTTP, 300 uM dCTP, and 1 unit Deep Vent polymerase. PCR reactions are performed in a Model 480 DNA thermal cycler (Perkin Elmer Corporation, Norwalk, Conn.). PCR reaction products are purified using a Wizard PCR Preps kit (Promega). 
     The primers are designed to include appropriate restriction enzyme recognition sites which allow for the cloning of the new gene into expression vectors. Typically “Fragment Start” is designed to create a NcoI restriction site, and “Fragment Stop” is designed to create a HindIII restriction site. Restriction digest reactions are purified using a Magic DNA Clean-up System kit (Promega). Fragments Start and Stop are resolved on a 1% TAE gel, stained with ethidium bromide and isolated using a Qiaex Gel Extraction kit (Qiagen). These fragments are combined with and annealed to the ends of the ˜3800 base pair NcoI/HindIII vector fragment of pMON3934 by heating at 50° C. for ten minutes and allowed to slow cool. The three fragments are ligated together using T4 DNA ligase (Boehringer Mannheim). The result is a plasmid containing the full-length new N-terminus/C-terminus gene. A portion of the ligation reaction is used to transform  E. coli  strain DH5αcells (Life Technologies, Gaithersburg, Md.). Plasmid DNA is purified and sequence confirmed as below. 
     Method III. Creation of new N-terminus/C-terminus genes by tandem-duplication method 
     New N-terminus/C-terminus genes can be made based on the method described in R. A. Horlick, et al  Protein Eng.  5:427-431 (1992). Polymerase chain reaction (PCR) amplification of the new N-terminus/C-terminus genes is performed using a tandemly duplicated template DNA. The steps are illustrated in FIG.  4 . 
     The tandemly-duplicated template DNA is created by cloning and contains two copies of the gene separated by DNA sequence encoding a linker connecting the original C- and N-terminal ends of the two copies of the gene. Specific primer sets are used to create and amplify a full-length new N terminus/C-terminus gene from the tandemly-duplicated template DNA. These primers are designed to include appropriate restriction sites which allow for the cloning of the new gene into expression vectors. Typical PCR conditions are one cycle 95° C. melting for two minutes; 25 cycles 94° C. denaturation for one minute, 50° C. annealing for one minute and 72° C. extension for one minute; plus one cycle 72° C. extension for seven minutes. A Perkin Elmer GeneAmp PCR Core Reagents kit (Perkin Elmer Corporation, Norwalk, Conn.) is used. A 100 ul reaction contains 100 pmole of each primer and one ug of template DNA; and 1×PCR buffer, 200 uM dGTP, 200 uM DATP, 200 uM dTTP, 200 uM dCTP, 2.5 units AmpliTaq DNA polymerase and 2 mM MgCl 2 . PCR reactions are performed in a Model 480 DNA thermal cycler (Perkin Elmer Corporation, Norwalk, Conn.). PCR reactions are purified using a Wizard PCR Preps kit (Promega). 
     DNA Isolation and Characterization 
     Plasmid DNA can be isolated by a number of different methods and using commercially available kits known to those skilled in the art. A few such methods are shown herein. Plasmid DNA is isolated using the Promega Wizard™ Miniprep kit (Madison, Wis.), the Qiagen QIAwell Plasmid isolation kits (Chatsworth, Calif.) or Qiagen Plasmid Midi kit. These kits follow the same general procedure for plasmid DNA isolation. Briefly, cells are pelleted by centrifugation (5000×g), plasmid DNA released with sequential NaOH/acid treatment, and cellular debris is removed by centrifugation (10000×g). The supernatant (containing the plasmid DNA) is loaded onto a column containing a DNA-binding resin, the column is washed, and plasmid DNA eluted with TE. After screening for the colonies with the plasmid of interest, the  E. coli  cells are inoculated into 50-100 mLs of LB plus appropriate antibiotic for overnight growth at 37° C. in an air incubator while shaking. The purified plasmid DNA is used for DNA sequencing, further restriction enzyme digestion, additional subcloning of DNA fragments and transfection into mammalian,  E. coli  or other cells. 
     Sequence Confirmation 
     Purified plasmid DNA is resuspended in dH 2 O and quantitated by measuring the absorbance at 260/280 nm in a Bausch and Lomb Spectronic 601 UV spectrometer. DNA samples are sequenced using ABI PRISM™ DyeDeoxy™ terminator sequencing chemistry (Applied Biosystems Division of Perkin Elmer Corporation, Lincoln City, Calif.) kits (Part Number 401388 or 402078) according to the manufacturers suggested protocol usually modified by the addition of 5% DMSO to the sequencing mixture. Sequencing reactions are performed in a Model 480 DNA thermal cycler (Perkin Elmer Corporation, Norwalk, Conn.) following the recommended amplification conditions. Samples are purified to remove excess dye terminators with Centri-Sep™ spin columns (Princeton Separations, Adelphia, N.J.) and lyophilized. Fluorescent dye labeled sequencing reactions are resuspended in deionized formamide, and sequenced on denaturing 4.75% polyacrylamide-8M urea gels using an ABI Model 373A automated DNA sequencer. Overlapping DNA sequence fragments are analyzed and assembled into master DNA contigs using Sequencher v2.1 DNA analysis software (Gene Codes Corporation, Ann Arbor, Mich.). 
     Expression of G-CSF Receptor Agonists in Mammalian Cells 
     Mammalian Cell Transfection/Production of Conditioned Media 
     The BHK-21 cell line can be obtained from the ATCC 10801 University Boulevard, Manassas, Va. 20110-2209. The cells are cultured in Dulbecco&#39;s modified Eagle media (DMEM/high-glucose), supplemented to 2 mM (mM) L-glutamine and 10% fetal bovine serum (FBS). This formulation is designated BHK growth media. Selective media is BHK growth media supplemented with 453 units/mL hygromycin B (Calbiochem, San Diego, Calif.). The BHK-21 cell line was previously stably transfected with the HSV transactivating protein VP16, which transactivates the IE110 promoter found on the plasmid pMON3359 (See Hippenmeyer et al.,  Bio/Technology,  pp.1037-1041, 1993). The VP16 protein drives expression of genes inserted behind the IE110 promoter. BHK-21 cells expressing the transactivating protein VP16 are designated BHK-VP16. The plasmid pMON1118 (See Highkin et al.,  Poultry Sci.,  70: 970-981, 1991) expresses the hygromycin resistance gene from the SV40 promoter. A similar plasmid is available from ATCC, pSV2-hph. 
     BHK-VP16 cells are seeded into a 60 millimeter (mm) tissue culture dish at 3×10 5  cells per dish 24 hours prior to transfection. Cells are transfected for 16 hours in 3 mL of “OPTIMEM”™ (Gibco-BRL, Gaithersburg, Md.) containing 10 ug of plasmid DNA containing the gene of interest, 3 ug hygromycin resistance plasmid, pMON1118, and 80 ug of Gibco-BRL “LIPOFECTAMINE”™ per dish. The media is subsequently aspirated and replaced with 3 mL of growth media. At 48 hours post-transfection, media from each dish is collected and assayed for activity (transient conditioned media). The cells are removed from the dish by trypsin-EDTA, diluted 1:10 and transferred to 100 mm tissue culture dishes containing 10 mL of selective media. After approximately 7 days in selective media, resistant cells grow into colonies several millimeters in diameter. The colonies are removed from the dish with filter paper (cut to approximately the same size as the colonies and soaked in trypsin/EDTA) and transferred to individual wells of a 24 well plate containing 1 mL of selective media. After the clones are grown to confluence, the conditioned media is re-assayed, and positive clones are expanded into growth media. 
     Expression of G-CSF Receptor Agonists in  E. coli    
       E. coli  strain MON105 or JM101 harboring the plasmid of interest are grown at 37° C. in M9 plus casamino acids medium with shaking in a air incubator Model G25 from New Brunswick Scientific (Edison, N.J.). Growth is monitored at OD600 until it reaches a value of 1, at which time nalidixic acid (10 milligrams/mL) in 0.1 N NaOH is added to a final concentration of 50 μg/mL. The cultures are then shaken at 37° C. for three to four additional hours. A high degree of aeration is maintained throughout culture period in order to achieve maximal production of the desired gene product. The cells are examined under a light microscope for the presence of inclusion bodies (IB). One mL aliquots of the culture are removed for analysis of protein content by boiling the pelleted cells, treating them with reducing buffer and electrophoresis via SDS-PAGE (see Maniatis et al. Molecular Cloning: A Laboratory Manual, 1982). The culture is centrifuged (5000×g) to pellet the cells. 
     Inclusion Body Preparation, Extraction, Refolding, Dialysis, DEAE Chromatography, and Characterization of the G-CSF Receptor Agonists which Accumulate as Inclusion Bodies in  E. coli    
     Isolation of Inclusion Bodies 
     The cell pellet from a 330 mL  E. coli  culture is resuspended in 15 mL of sonication buffer (10 mM 2-amino-2-(hydroxymethyl) 1,3-propanediol hydrochloride (Tris-HCl), pH 8.0+1 mM ethylenediaminetetraacetic acid (EDTA)). These resuspended cells are sonicated using the microtip probe of a Sonicator Cell Disruptor (Model W-375, Heat Systems-Ultrasonics, Inc., Farmingdale, N.Y.). Three rounds of sonication in sonication buffer followed by centrifugation are employed to disrupt the cells and wash the inclusion bodies (IB). The first round of sonication is a 3 minute burst followed by a 1 minute burst, and the final two rounds of sonication are for 1 minute each. 
     Extraction and Refolding of Proteins from Inclusion Body Pellets 
     Following the final centrifugation step, the IB pellet is resuspended in 10 mL of 50 mM Tris-HCl, pH 9.5, 8 M urea and 5 mM dithiothreitol (DTT) and stirred at room temperature for approximately 45 minutes to allow for denaturation of the expressed protein. 
     The extraction solution is transferred to a beaker containing 70 mL of 5mM Tris-HCl, pH 9.5 and 2.3 M urea and gently stirred while exposed to air at 4° C. for 18 to 48 hours to allow the proteins to refold. Refolding is monitored by analysis on a Vydac (Hesperia, Calif.) C18 reversed phase high pressure liquid chromatography (RP-HPLC) column (0.46×25 cm). A linear gradient of 40% to 65% acetonitrile, containing 0.1% trifluoroacetic acid (TFA), is employed to monitor the refold. This gradient is developed over 30 minutes at a flow rate of 1.5 mL per minute. Denatured proteins generally elute later in the gradient than the refolded proteins. 
     Purification 
     Following the refold, contaminating  E. coli  proteins are removed by acid precipitation. The pH of the refold solution is titrated to between pH 5.0 and pH 5.2 using 15% (v/v) acetic acid (HOAc). This solution is stirred at 4° C. for 2 hours and then centrifuged for 20 minutes at 12,000×g to pellet any insoluble protein. 
     The supernatant from the acid precipitation step is dialyzed using a Spectra/Por 3 membrane with a molecular weight cut off (MWCO) of 3,500 daltons. The dialysis is against 2 changes of 4 liters (a 50-fold excess) of 10 mM Tris-HCl, pH 8.0 for a total of 18 hours. Dialysis lowers the sample conductivity and removes urea prior to DEAE chromatography. The sample is then centrifuged (20 minutes at 12,000×g) to pellet any insoluble protein following dialysis. 
     A Bio-Rad Bio-Scale DEAE2 column (7×52 mm) is used for ion exchange chromatography. The column is equilibrated in a buffer containing 10 mM Tris-HCl, pH 8.0. The protein is eluted using a 0-to-500 mM sodium chloride (NaCl) gradient, in equilibration buffer, over 45 column volumes. A flow rate of 1 mL per minute is used throughout the run. Column fractions (2 mL per fraction) are collected across the gradient and analyzed by RP HPLC on a Vydac (Hesperia, Calif.) C18 column (0.46×25 cm). A linear gradient of 40% to 65% acetonitrile, containing 0.1% trifluoroacetic acid (TFA), is employed. This gradient is developed over 30 minutes at a flow rate of 1.5 mL per minute. Pooled fractions are then dialyzed against 2 changes of 4 liters (50-to-500-fold excess) of 10 mM ammonium acetate (NH 4 Ac), pH 4.0 for a total of 18 hours. Dialysis is performed using a Spectra/Por 3 membrane with a MWCO of 3,500 daltons. Finally, the sample is sterile filtered using a 0.22 μm syringe filter (μStar LB syringe filter, Costar, Cambridge, Mass.), and stored at 4° C. 
     In some cases the folded proteins can be affinity purified using affinity reagents such as mAbs or receptor subunits attached to a suitable matrix. Alternatively, (or in addition) purification can be accomplished using any of a variety of chromatographic methods such as: ion exchange, gel filtration or hydrophobic chromatography or reversed phase HPLC. 
     These and other protein purification methods are described in detail in Methods in Enzymology, Volume 182 ‘Guide to Protein Purification’ edited by Murray Deutscher, Academic Press, San Diego, Calif. (1990). 
     Protein Characterization 
     The purified protein is analyzed by RP-HPLC, electrospray mass spectrometry, and SDS-PAGE. The protein quantitation is done by amino acid composition, RP-HPLC, and Bradford protein determination. In some cases tryptic peptide mapping is performed in conjunction with electrospray mass spectrometry to confirm the identity of the protein. 
     AML Proliferation Assay 
     The factor-dependent cell line AML 193 was obtained from the American Type Culture Collection (ATCC, Rockville, Md.). This cell line, established from a patient with acute myelogenous leukemia, is a growth factor dependent cell line which displayed enhanced growth in GM-CSF supplemented medium (Lange, B., et al.,  Blood  70: 192, 1987; Valtieri, M., et al.,  J. Immunol.  138:4042, 1987). The ability of AML 193 cells to proliferate in the presence of human IL-3 has also been documented. (Santoli, D., et al.,  J. Immunol.  139: 348, 1987). A cell line variant was used, AML 193 1.3, which was adapted for long term growth in IL-3 by washing out the growth factors and starving the cytokine dependent AML 193 cells for growth factors for 24 hours. The cells are then replated at 1×10 5  cells/well in a 24 well plate in media containing 100 U/mL IL-3. It took approximately 2 months for the cells to grow rapidly in IL-3. These cells are maintained as AML 193 1.3 thereafter by supplementing tissue culture medium (see below) with human IL-3. 
     AML 193 1.3 cells are washed 6 times in cold Hanks balanced salt solution (HBSS, Gibco, Grand Island, N.Y.) by centrifuging cell suspensions at 250×g for 10 minutes followed by decantation of the supernatant. Pelleted cells are resuspended in HBSS and the procedure is repeated until six wash cycles are completed. Cells washed six times by this procedure are resuspended in tissue culture medium at a density ranging from 2×10 5  to 5×10 5  viable cells/mL. This medium is prepared by supplementing Iscove&#39;s modified Dulbecco&#39;s Medium (IMDM, Hazelton, Lenexa, Kans.) with albumin, transferrin, lipids and 2-mercaptoethanol. Bovine albumin (Boehringer-Mannheim, Indianapolis, Ind.) is added at 500 μg/mL; human transferrin (Boehringer-Mannheim, Indianapolis, Ind.) is added at 100 μg/mL; soybean lipid (Boehringer-Mannheim, Indianapolis, Ind.) is added at 50 μg/mL; and 2-mercaptoethanol (Sigma, St. Louis, Mo.) is added at 5×10 −5  M. 
     Serial dilutions of G-CSF receptor agonist proteins are made in triplicate series in tissue culture medium supplemented as stated above in 96 well Costar 3596 tissue culture plates. Each well contained 50 μl of medium containing G-CSF receptor agonist proteins once serial dilutions are completed. Control wells contained tissue culture medium alone (negative control). AML 193 1.3 cell suspensions prepared as above are added to each well by pipetting 50 μl (2.5×10 4  cells) into each well. Tissue culture plates are incubated at 37° C. with 5% CO 2  in humidified air for 3 days. On day 3, 0.5 μCi  3 H-thymidine (2 Ci/mM, New England Nuclear, Boston, Mass.) is added in 50 μl of tissue culture medium. Cultures are incubated at 37° C. with 5% CO 2  in humidified air for 18-24 hours. Cellular DNA is harvested onto glass filter mats (Pharmacia LKB, Gaithersburg, Md.) using a TOMTEC cell harvester (TOMTEC, Orange, Conn.) which utilized a water wash cycle followed by a 70% ethanol wash cycle. Filter mats are allowed to air dry and then placed into sample bags to which scintillation fluid (Scintiverse II, Fisher Scientific, St. Louis, Mo. or BetaPlate Scintillation Fluid, Pharmacia LKB, Gaithersburg, Md.) is added. Beta emissions of samples from individual tissue culture wells are counted in a LKB BetaPlate model 1205 scintillation counter (Pharmacia LKB, Gaithersburg, Md.) and data is expressed as counts per minute of  3 H-thymidine incorporated into cells from each tissue culture well. Activity of each G-CSF receptor agonist proteins preparation is quantitated by measuring cell proliferation ( 3 H-thymidine incorporation) induced by graded concentrations of G-CSF receptor agonist. Typically, concentration ranges from 0.05 pM-10 5  pM are quantitated in these assays. Activity is determined by measuring the dose of G-CSF receptor agonist protein which provides 50% of maximal proliferation (EC 50   =0.5×(maximum average counts per minute of    3 H-thymidine incorporated per well among triplicate cultures of all concentrations of G-CSF receptor agonists tested—background proliferation measured by  3 H-thymidine incorporation observed in triplicate cultures lacking any factor). This EC 50  value is also equivalent to 1 unit of bioactivity. Every assay is performed with native interleukin-3 and G-CSF as reference standards so that relative activity levels could be assigned. 
     Typically, the G-CSF receptor agonist proteins were tested in a concentration range of 2000 pM to 0.06 pM titrated in serial 2 fold dilutions. 
     Activity for each sample was determined by the concentration which gave 50% of the maximal response by fitting a four-parameter logistic model to the data. It was observed that the upper plateau (maximal response) for the sample and the standard with which it was compared did not differ. Therefore relative potency calculation for each sample was determined from EC50 estimations for the sample and the standard as indicated above. 
     Other in vitro Cell Based Proliferation Assays 
     Other in vitro cell based proliferation assays, known to those skilled in the art, may also be useful to determine the activity of the G-CSF receptor agonists in a similar manner as described in the AML 193.1.3 cell proliferation assay. 
     Transfected Cell Lines 
     Cell lines, such as BHK or the murine pro B cell line Baf/3, can be transfected with a colony stimulating factor receptor, such as the human G-CSF receptor which the cell line does not have. These transfected cell lines can be used to determine the activity of the ligand of which the receptor has been transfected. 
     EXAMPLE 1 
     Construction of pMON3485 
     The new N-terminus/C-terminus gene in pMON3485 was created using Method I as described in Materials and Methods. Fragment Start was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 39 start (SEQ ID NO:7) and L-11 start (SEQ ID NO:3). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in the plasmid, pMON13037 (WO 95/21254), using the primer set, 38 stop (SEQ ID NO:8) and L-11 stop (SEQ ID NO:4). The full-length new N terminus/C-terminus G-CSF Ser 17  gene was created and amplified from the annealed Fragments Start and Stop using the primers 39 start (SEQ ID NO:7) and 38 stop (SEQ ID NO:8). 
     The resulting DNA fragment which contains the new gene was digested with restriction endonucleases NcoI and HindIII and purified using a Magic DNA Clean-up System kit (Promega, Madison, Wis.). The plasmid, pMON3934 (derivative of pMON3359), was digested with restriction endonucleases HindIII and NcoI, resulting in an approximately 3800 base pair vector fragment, and gel-purified. The purified restriction fragments were combined and ligated using T4 DNA ligase. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells (Life Technologies, Gaithersburg, Md.). Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3485. 
     BHK cells were transfected with the plasmid, pMON3485, for protein expression and bioassay. 
     The plasmid, pMON3485 containing the gene sequence of (SEQ ID NO:25), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:43) 
               
             
          
           
               
                 Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
               
               
                   
               
               
                 Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser 
               
               
                   
               
               
                 Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys 
               
               
                   
               
               
                 Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln 
               
               
                   
               
               
                 Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu 
               
               
                   
               
               
                 Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val 
               
               
                   
               
               
                 Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu 
               
               
                   
               
               
                 Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln 
               
               
                   
               
               
                 Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg 
               
               
                   
               
               
                 Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln 
               
               
                   
               
               
                 Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His 
               
               
                   
               
               
                 Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly Ser Gln 
               
               
                   
               
               
                 Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys 
               
               
                   
               
               
                 Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu 
               
               
                   
               
               
                 Cys Ala Thr 
               
             
          
         
       
     
     EXAMPLE 2 
     Construction of pMON3486 
     The new N-terminus/C-terminus gene in pMON3486 was created using Method I as described in Materials and Methods. Fragment Start was created and amplified from G-CSF Serl 7  sequence in the plasmid, pMON13037, using the primer set, 97 start (SEQ ID NO:9) and L-11 start (SEQ ID NO:3). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 96 stop (SEQ ID NO:10) and L-11 stop (SEQ ID NO:4). The full-length new N terminus/C-terminus G-CSF Ser 17  gene was created and amplified from the annealed Fragments Start and Stop using the primers 97 start (SEQ ID NO:9) and 96 stop (SEQ ID NO:10). 
     The resulting DNA fragment which contains the new gene was digested with restriction endonucleases NcoI and HindIII and gel-purified using a Magic DNA Clean-up System kit. The plasmid, pMON3934, was digested with restriction endonucleases HindIII and NcoI, resulting in an approximately 3800 base pair vector fragment, and gel-purified. The purified restriction fragments were combined and ligated using T4 DNA ligase. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3486. 
     BHK cells were transfected with the plasmid, pMON3486, for protein expression and bioassay. 
     The plasmid, pMON3486 containing the gene sequence of (SEQ ID NO:26), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:44) 
               
             
          
           
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly 
               
               
                   
               
               
                 Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val 
               
               
                   
               
               
                 Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu 
               
               
                   
               
               
                 Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu 
               
               
                   
               
               
                 Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro 
               
               
                   
               
               
                 Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu 
               
               
                   
               
               
                 Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly 
               
               
                   
               
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser 
               
             
          
         
       
     
     EXAMPLE 3 
     Construction of pMON3487 
     The new N-terminus/C-terminus gene in pMON3487 was created using Method I as described in Materials and Methods. Fragment Start was created and amplified from G-CSF Ser 17  sequence in the plasmid, pMON13037, using the primer set, 126 start (SEQ ID NO:11) and L-11 start (SEQ ID NO:3). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 125 stop (SEQ ID NO:12) and L-11 stop (SEQ ID NO:4). The full-length new N terminus/C-terminus G-CSF Ser 17  gene was created and amplified from the annealed Fragments Start and Stop using the primers 126 start (SEQ ID NO:11) and 125 stop (SEQ ID NO:12). 
     The resulting DNA fragment which contains the new gene was digested with restriction endonucleases NcoI and HindIII and purified using a Magic DNA Clean-up System kit. The plasmid, pMON3934, was digested with restriction endonucleases HindIII and NcoI, resulting in an approximately 3800 base pair vector fragment, and gel-purified. The purified restriction fragments were combined and ligated using T4 DNA ligase. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3487. 
     BHK cells were transfected with the plasmid, pMON3487, for protein expression and bioassay. 
     The plasmid, pMON3487 containing the gene sequence of (SEQ ID NO:27), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:45) 
               
             
          
           
               
                 Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met 
               
               
                   
               
               
                 Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly 
               
               
                   
               
               
                 Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu 
               
               
                   
               
               
                 Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln 
               
               
                   
               
               
                 Pro Ser Gly Gly Ser Gly Gly Ser Gln Ser Phe Leu 
               
               
                   
               
               
                 Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly 
               
               
                   
               
               
                 Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr 
               
               
                   
               
               
                 Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
               
               
                   
               
               
                 Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser 
               
               
                   
               
               
                 Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys 
               
               
                   
               
               
                 Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln 
               
               
                   
               
               
                 Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu 
               
               
                   
               
               
                 Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val 
               
               
                   
               
               
                 Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu 
               
               
                   
               
               
                 Glu Leu Gly 
               
             
          
         
       
     
     EXAMPLE 4 
     Construction of pMON3488 
     The new N-terminus/C-terminus gene in pMON3488 was created using Method I as described in Materials and Methods. Fragment Start was created and amplified from G-CSF Ser 17  sequence in the plasmid, pMON13037, using the primer set, 133 start (SEQ ID NO:13) and L-11 start (SEQ ID NO:3). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in the plasmid, pMON13037 using the primer set, 132 stop (SEQ ID NO:14) and L-11 stop (SEQ ID NO:4). The full-length new N terminus/C-terminus G-CSF Ser 17  gene was created and amplified from the annealed Fragments Start and Stop using the primers 133 start (SEQ ID NO:13) and 132 stop (SEQ ID NO:14). 
     The resulting DNA fragment which contains the new gene was digested with restriction endonucleases NcoI and HindIII and purified using a Magic DNA Clean-up System kit. The plasmid, pMON3934, was digested with restriction endonucleases HindIII and NcoI, resulting in an approximately 3800 base pair vector fragment, and gel-purified. The purified restriction fragments were combined and ligated using T4 DNA ligase. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3488. 
     BHK cells were transfected with the plasmid, pMON3488, for protein expression and bioassay. 
     The plasmid, pMON3488 containing the gene sequence of (SEQ ID NO:28), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:46) 
               
             
          
           
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly 
               
               
                   
               
               
                 Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val 
               
               
                   
               
               
                 Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu 
               
               
                   
               
               
                 Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu 
               
               
                   
               
               
                 Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro 
               
               
                   
               
               
                 Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu 
               
               
                   
               
               
                 Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly 
               
               
                   
               
               
                 Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu 
               
               
                   
               
               
                 Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr 
               
               
                   
               
               
                 Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile 
               
               
                   
               
               
                 Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala 
               
               
                   
               
               
                 Leu Gln Pro 
               
             
          
         
       
     
     EXAMPLE 5 
     Construction of pMON3489 
     The new N-terminus/C-terminus gene in pMON3489 was created using Method I as described in Materials and Methods. Fragment Start was created and amplified from G-CSF Ser 17  sequence in the plasmid, pMON13037, using the primer set, 142 start (SEQ ID NO:15) and L-11 start (SEQ ID NO:3). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 141 stop (SEQ ID NO:16) and L-11 stop (SEQ ID NO:4). The full-length new N terminus/C-terminus G-CSF Ser 17  gene was created and amplified from the annealed Fragments Start and Stop using the primers 142 start (SEQ ID NO:15) and 141 stop (SEQ ID NO:16). 
     The resulting DNA fragment which contains the new gene was digested with restriction endonucleases NcoI and HindIII and purified using a Magic DNA Clean-up System kit. The plasmid, pMON3934, was digested with restriction endonucleases HindIII and NcoI, resulting in an approximately 3800 base pair vector fragment, and gel-purified. The purified restriction fragments were combined and ligated using T4 DNA ligase. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3489. 
     BHK cells were transfected with the plasmid, pMON3489, for protein expression and bioassay. 
     The plasmid, pMON3489 containing the gene sequence of (SEQ ID NO:29), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:47) 
               
             
          
           
               
                 Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val 
               
               
                   
               
               
                 Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr 
               
               
                   
               
               
                 Arg Val Leu Arg His Leu Ala Gln Pro Ser Gly Gly 
               
               
                   
               
               
                 Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr 
               
               
                   
               
               
                 Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
               
               
                   
               
               
                 Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met 
               
               
                   
               
               
                 Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro 
               
               
                   
               
               
                 Ala Phe Ala 
               
             
          
         
       
     
     EXAMPLE 6 
     Construction of pMON3490 
     The new N-terminus/C-terminus gene in pMON3490 was created using Method II as described in Materials and Methods. Fragment Start was created and amplified from G-CSF sequence in the plasmid, pMON13037, using the primer set, 39 start (SEQ ID NO:7) and P-bl start (SEQ ID NO:5). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 38 stop (SEQ ID NO:8) and P-bl stop (SEQ ID NO:6). Fragment Start was digested with restriction endonuclease NcoI, and Fragment Stop was digested with restriction endonuclease HindIII. After purification, the digested Fragments Start and Stop were combined with and ligated to the approximately 3800 base pair NcoI-HindIII vector fragment of pMON3934. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3490. 
     BHK cells were transfected with the plasmid, pMON3490, for protein expression and bioassay. 
     The plasmid, pMON3490 containing the gene sequence of (SEQ ID NO:30), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:48) 
               
             
          
           
               
                 Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
               
               
                   
               
               
                 Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser 
               
               
                   
               
               
                 Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys 
               
               
                   
               
               
                 Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln 
               
               
                   
               
               
                 Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu 
               
               
                   
               
               
                 Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val 
               
               
                   
               
               
                 Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu 
               
               
                   
               
               
                 Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln 
               
               
                   
               
               
                 Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg 
               
               
                   
               
               
                 Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln 
               
               
                   
               
               
                 Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His 
               
               
                   
               
               
                 Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser 
               
               
                   
               
               
                 Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln 
               
               
                   
               
               
                 Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln 
               
               
                   
               
               
                 Glu Lys Leu Cys Ala Thr 
               
             
          
         
       
     
     EXAMPLE 7 
     Construction of pMON3491 
     The new N-terminus/C-terminus gene in pMON3491 was created using Method II as described in Materials and Methods. Fragment Start was created and amplified from G-CSF sequence in the plasmid, pMON13037, using the primer set, 97 start (SEQ ID NO:9) and P-bl start (SEQ ID NO:5). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 96 stop (SEQ ID NO:10) and P-bl stop (SEQ ID NO:6). Fragment Start was digested with restriction endonuclease NcoI, and Fragment Stop was digested with restriction endonuclease HindIII. After purification, the digested Fragments Start and Stop were combined with and ligated to the approximately 3800 base pair NcoI-HindIII vector fragment of pMON3934. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3491. 
     BHK cells were transfected with the plasmid, pMON3491, for protein expression and bioassay. 
     The plasmid, pMON3491 containing the gene sequence of (SEQ ID NO:31), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:49) 
               
             
          
           
               
                 Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu 
               
               
                   
               
               
                 Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln 
               
               
                   
               
               
                 Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro 
               
               
                   
               
               
                 Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser 
               
             
          
         
       
     
     EXAMPLE 8 
     Construction of pMON3492 
     The new N-terminus/C-terminus gene in pMON3492 was created using Method II as described in Materials and Methods. Fragment Start was created and amplified from G-CSF sequence in the plasmid, pMON13037, using the primer set, 126 start (SEQ ID NO:11) and P-bl start (SEQ ID NO:5). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 125 stop (SEQ ID NO:12) and P-bl stop (SEQ ID NO:6). Fragment Start was digested with restriction endonuclease NcoI, and Fragment Stop was digested with restriction endonuclease HindIII. After purification, the digested Fragments Start and Stop were combined with and ligated to the approximately 3800 base pair NcoI-HindIII vector fragment of pMON3934. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3492. 
     BHK cells were transfected with the plasmid, pMON3492, for protein expression and bioassay. 
     The plasmid, pMON3492 containing the gene sequence of (SEQ ID NO:32), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:50) 
               
             
          
           
               
                 Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met 
               
               
                   
               
               
                 Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly 
               
               
                   
               
               
                 Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu 
               
               
                   
               
               
                 Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln 
               
               
                   
               
               
                 Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln 
               
               
                   
               
               
                 Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys 
               
               
                   
               
               
                 Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu 
               
               
                   
               
               
                 Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
               
               
                   
               
               
                 Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala 
               
               
                   
               
               
                 Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu 
               
               
                   
               
               
                 Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe 
               
               
                   
               
               
                 Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
               
               
                   
               
               
                 Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln 
               
               
                   
               
               
                 Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln 
               
               
                   
               
               
                 Gln Met Glu Glu Leu Gly 
               
             
          
         
       
     
     EXAMPLE 9 
     Construction of pMON3493 
     The new N-terminus/C-terminus gene in pMON3493 was created using Method II as described in Materials and Methods. Fragment Start was created and amplified from G-CSF sequence in the plasmid, pMON13037, using the primer set, 133 start (SEQ ID NO:13) and P-bl start (SEQ ID NO:5). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 132 stop (SEQ ID NO:14) and P-bl stop (SEQ ID NO:6). Fragment Start was digested with restriction endonuclease NcoI, and Fragment Stop was digested with restriction endonuclease HindIII. After purification, the digested Fragments Start and Stop were combined with and ligated to the approximately 3800 base pair NcoI-HindIII vector fragment of pMON3934. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3493. 
     BHK cells were transfected with the plasmid, pMON3493, for protein expression and bioassay. 
     The plasmid, pMON3493 containing the gene sequence of (SEQ ID NO:33), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:51) 
               
             
          
           
               
                 Thr Gln Gly Ala Met Pro Ala Fhe Ala Ser Ala Phe 
               
               
                   
               
               
                 Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His 
               
               
                   
               
               
                 Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu 
               
               
                   
               
               
                 Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala 
               
               
                   
               
               
                 Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu 
               
               
                   
               
               
                 Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala 
               
               
                   
               
               
                 Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys 
               
               
                   
               
               
                 His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu 
               
               
                   
               
               
                 Gly Ile Pro Trp Ala Pro Leu Ser ser Cys Pro Ser 
               
               
                   
               
               
                 Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu 
               
               
                   
               
               
                 His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln 
               
               
                   
               
               
                 Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr 
               
               
                   
               
               
                 Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
               
               
                   
               
               
                 Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met 
               
               
                   
               
               
                 Ala Pro Ala Leu Gln Pro 
               
             
          
         
       
     
     EXAMPLE 10 
     Construction of pMON3494 
     The new N-terminus/C-terminus gene in pMON3494 was created using Method II as described in Materials and Methods. Fragment Start was created and amplified from G-CSF sequence in the plasmid, pMON13037, using the primer set, 142 start (SEQ ID NO:15) and P-bl start (SEQ ID NO:5). Fragment Stop was created and amplified from G-CSF Ser 17  sequence in pMON13037 using the primer set, 141 stop (SEQ ID NO:16) and P-bl stop (SEQ ID NO:6). Fragment Start was digested with restriction endonuclease NcoI, and Fragment Stop was digested with restriction endonuclease HindIII. After purification, the digested Fragments Start and Stop were combined with and ligated to the approximately 3800 base pair NcoI-HindIII vector fragment of pMON3934. A portion of the ligation reaction was used to transform  E. coli  strain DH5α cells. Transformant bacteria were selected on ampicillin-containing plates. Plasmid DNA was isolated and sequenced to confirm the correct insert. The resulting plasmid was designated pMON3494. 
     BHK cells were transfected with the plasmid, pMON3494, for protein expression and bioassay. 
     The plasmid, pMON3494 containing the gene sequence of (SEQ ID NO:34), encodes the following amino acid sequence: 
     
       
         
               
               
             
               
             
           
               
                   
                 (SEQ ID NO:52) 
               
             
          
           
               
                 Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val 
               
               
                   
               
               
                 Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr 
               
               
                   
               
               
                 Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu 
               
               
                   
               
               
                 Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
               
               
                   
               
               
                 Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp 
               
               
                   
               
               
                 Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr 
               
               
                   
               
               
                 Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly 
               
               
                   
               
               
                 His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
               
               
                   
               
               
                 Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu 
               
               
                   
               
               
                 Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly 
               
               
                   
               
               
                 Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu 
               
               
                   
               
               
                 Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
               
               
                   
               
               
                 Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu 
               
               
                   
               
               
                 Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly 
               
               
                   
               
               
                 Ala Met Pro Ala Phe Ala 
               
             
          
         
       
     
     EXAMPLES 11-20 
     The genes encoding the G-CSF receptor agonists of Examples 1-10 were excised from the BHK vectors as a NcoI/HindIII fragment and ligated with the ˜3630 base pair NcoI/HindIII vector fragment of pMON2341 (WO 94/12638). The resulting plasmids (Examples 11-20) are indicated in Table 4. The plasmids were transformed into  E. coli  strain JM101 cells and expression of the G-CSF receptor agonist protein was evaluated. The proteins expressed are the same as those expressed in the parental BHK expression vector except the proteins were immediately preceded by a Methionine-Alanine dipeptide and the Methionine is processed off by methionine aminopeptidase. Overnight growths of cells (20 Klett units) were inoculated in 10 mL of minimal M9 medium supplemented with vitamin B1 and trace minerals and incubated with shaking at 37° C. until initial Klett readings of ˜120 units were obtained. At 120 Klett units 5 uL of 10 mg/mL nalidixic acid was added. Four hours post-induction, a 1 ml aliquot was removed for protein expression analysis by SDS-PAGE. Cells were also examined using light microscopy for the presence of inclusion bodies. Only pMON3450 and pMON3455 had significant expression levels of the G-CSF receptor agonist protein. In an effort to improve expression levels of G-CSF receptor agonists, the 5′ end of the genes were re-engineered to incorporate AT-rich codons and  E. coli  preferred codons between the unique NcoI and NheI restriction endonuclease recognition sites (Examples 21-28). 
     
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                   E. coli  expression plasmids 
               
             
          
           
               
                   
                 Resulting 
                   
                   
                   
               
               
                   
                 
                   E. coli 
                 
               
               
                   
                 expression 
                   
                   
                 Parental 
               
               
                   
                 plasmid 
                 Break- 
                   
                 BHK plasmid 
               
               
                 Example # 
                 pMON# 
                 point 
                 Linker 
                 pMON# 
               
               
                   
               
               
                 Example 11 
                 pMON3450 
                 38/39 
                 zero 
                 pMON3490 
               
               
                 Example 12 
                 pMON3455 
                 38/39 
                 Δ1-10 
                 pMON3485 
               
               
                 Example 13 
                 pMON3451 
                 96/97 
                 zero 
                 pMON3491 
               
               
                 Example 14 
                 pMON3456 
                 96/97 
                 Δ1-10 
                 pMON3486 
               
               
                 Example 15 
                 pMON3452 
                 125/126 
                 zero 
                 pMON3492 
               
               
                 Example 16 
                 pMON3457 
                 125/126 
                 Δ1-10 
                 pMON3487 
               
               
                 Example 17 
                 pMON3453 
                 132/133 
                 zero 
                 pMON3493 
               
               
                 Example 18 
                 pMON3458 
                 132/133 
                 Δ1-10 
                 pMCN3488 
               
               
                 Example 19 
                 pMON3454 
                 141/142 
                 zero 
                 pMON3494 
               
               
                 Example 20 
                 pMON3459 
                 141/142 
                 Δ1-10 
                 pMON3489 
               
               
                   
               
             
          
         
       
     
     EXAMPLE 21 
     Construction of pMON25184 
     The complementary pair of synthetic oligomers, 141for.seq (SEQ ID NO:23) and 141rev.seq (SEQ ID NO:24), (Midland Certified Reagent Co., Midland Tex.) were annealed by heating 2 ug of each synthetic oligomer in a 20 ul reaction mixture containing 20 mM Tris-HCl (7.5), 10 mM MgCl 2 , and 50 mM NaCl, at 80° C. for 5 minutes, and allowing the mixture to slowly cool to ambient temperature (approximately 45 minutes). When properly annealed the oligomers create an NcoI site at the 5′ end and a NheI site at the 3′ end. Approximately 15 ng of the annealed oligomer pair was ligated with the gel-purified ˜4120 base pair NcoI/NheI vector fragment of pMON3454 (˜molar ratio of 10:1). The resulting gene, had seven codon changes at the 5′ end of the gene. The ligation reaction was used to transform  E. coli  strain DH5α and the desired codon changes were confirmed by DNA sequence analysis. The resulting plasmid was designated pMON25184. Plasmid, pMON25184 containing the gene sequence of (SEQ ID NO:38), DNA was retransformed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3454. 
     EXAMPLE 22 
     Construction of PMON25188 
     The complementary pair of synthetic oligomers, 141for.seq (SEQ ID NO:23) and 141rev.seq (SEQ ID NO:24), (Midland Certified Reagent Co., Midland Tex.) were annealed by heating 2 ug of each synthetic oligomer in a 20 ul reaction mixture containing 20 mM Tris-HCl (7.5), 10 mM MgCl 2 , and 50 mM NaCl, at 80° C. for 5 minutes, and allowing the mixture to slowly cool to ambient temperature (approximately 45 minutes). When properly annealed the oligomers create an NcoI site at the 5′ end and a NheI site at the 3′ end. Approximately 15 ng of the annealed oligomer pair was ligated with the ˜4110 base pair NcoI/NheI gel-purified pMON3459 (˜molar ratio of 10:1). The ligation mixture was used to transform  E. coli  strain DH5α and the desired codon changes were confirmed by DNA sequence analysis. The resulting plasmid was designated pMON25188. The resulting gene, had seven codon changes at the 5′ end of the gene. Plasmid, pMON25188 containing the gene sequence of (SEQ ID NO:42), DNA was retransformed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3459. 
     EXAMPLE 23 
     Construction of pMON25183 
     pMON25183 was constructed using an overlapping PCR primer method. The synthetic oligomers, 132for.seq (SEQ ID NO:321 and 132rev.seq (SEQ ID NO:22), encode the NcoI and NheI restriction recognition sequence, respectively. Amplified DNA was generated by the DNA polymerase chain amplification method using the PCR Optimizer Kit (Invitrogen). The PCR reactions were performed using the manufacturer&#39;s recommended conditions using 5×buffer B (300 mM Tris-HCl pH8.5, 75 mM (NH 4 ) 2 SO 4 , 10 mM MgCl 2 ) for seven cycles consisting of 94° C. for 1′, 65° C. for 2′, and 72° C. for 2′, followed by 20 cycles of 94° C. for 1′, and 72° C. for 3′, and a final cycle of 7 minutes at 72° C. using a Perkin Elmer Model 480 DNA thermal cycler (Perkin Elmer). The reaction product was desalted using Centri-Sep spin columns (Princeton Separations) following the manufacturer&#39;s recommended protocol, digested with NcoI/NheI, and gel purified from TAE-agarose gels using Gene Clean (Bio 101) and the DNA product was eluted in dH 2 O The purified PCR product was ligated with the ˜4090 base pair NcoI/NheI pMON3453 vector fragment. Positive clones containing the AT-rich replacement insert were identified as described in Example 21. The resulting plasmid was designated pMON25183. The resulting gene, had 14 codon changes at the 5′ end of the gene. Plasmid, pMON25183 containing the gene sequence of (SEQ ID NO:37), DNA was retransformed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3453. 
     EXAMPLE 24 
     Construction of pMON25187 
     pMON25187 was constructed using an overlapping PCR primer method. The synthetic oligomers, 132for.seq (SEQ ID NO:21) and 132rev.seq (SEQ ID NO:22), encode the NcoI and NheI restriction recognition sequence, respectively. Amplified DNA was generated by the DNA polymerase chain amplification method using the PCR Optimizer Kit (Invitrogen). The PCR reactions were performed using the manufacturer&#39;s recommended conditions, in 5×buffer B for seven cycles consisting of 94° C. for 1′, 65° C. for 2′, and 72° C. for 2′, followed by 20 cycles of 94° C. for 1′, and 72° C. for 3′, and a final cycle of 7 minutes at 72° C. using a Perkin Elmer Model 480 DNA thermal cycler (Perkin Elmer). The reaction product was desalted using Centri-Sep spin columns (Princeton Separations) following the manufacturer&#39;s recommended protocol, digested with NcoI/NheI, and gel purified from TAE-agarose gels using Gene Clean (Bio 101) and the DNA product was eluted in dH 2 O. The purified PCR product was ligated with the ˜4080 base pair NcoI/NheI pMON3458 vector fragment. Positive clones containing the AT-rich replacement insert were identified as described in Example 21. The resulting plasmid was designated pMON25187. The resulting gene, had 14 codon changes at the 5′ end of the gene. Plasmid, pMON25187 containing the gene sequence of (SEQ ID NO:41), DNA was retransformed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3458. 
     EXAMPLE 25 
     Construction of pMON25182 
     pMON25182 was constructed using the overlapping PCR primer approach described in Example 23. The synthetic oligomer primers 125for.seq (SEQ ID NO:19) and 125rev.seq (SEQ ID NO:20) were used in the PCR reaction. The PCR reaction conditions were identical to those used in Example 23 except the annealing temperature for the first seven cycles was 60° C. The purified PCR product was ligated with ˜4070 base pair NcoI/NheI pMON3452 vector fragment. Positive clones containing the AT-rich replacement insert were identified a s described in Example 21. The resulting plasmid was designated pMON25182. The resulting gene, had 19 codon changes at the 5′ end of the gene. Plasmid, pMON25182 containing the gene sequence of (SEQ ID NO:36), DNA was retransfomed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3452. 
     EXAMPLE 26 
     Construction of pMON25186 
     pMON25186 was constructed using the overlapping PCR primer approach described in Example 23. The synthetic oligomer primers 125for.seq (SEQ ID NO:19) and 125rev.seq (SEQ ID NO:20) were used in the PCR reaction. The PCR reaction conditions were identical to those used in Example 23 except the annealing temperature for the first seven cycles was 60° C. The purified PCR product was ligated with the ˜4060 base pair NcoI/NheI pMON3457 vector fragment. Positive clones containing the AT-rich replacement insert were identified as described in Example 21. The resulting plasmid was designated pMON25186. The resulting gene, had 19 codon changes at the 5′ end of the gene. Plasmid, pMON25186 containing the gene sequence of (SEQ ID NO:40), DNA was retransformed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3457. 
     EXAMPLES 27 
     Construction of pMON25181 
     pMON25181 was constructed using PCR to amplify a DNA fragment from pMON3451 as the template using the oligomers 96for.seq (SEQ ID NO:17) and 96rev.seq (SEQ ID NO:18). The oligomer 96for.seq was designed to create six codon changes. The PCR reaction conditions were the same as described in Example 25, except 10 ng of pMON3451 plasmid DNA was added. The purified PCR product was ligated with the ˜3980 base pair NcoI/NheI pMON3451 vector fragment. Positive clones containing the AT-rich replacement insert were identified as described in Example 21. The resulting plasmid was designated pMON25181. The resulting gene, had 6 codon changes at the 5′ end of the gene. Plasmid, pMON25181 containing the gene sequence of (SEQ ID NO:35), DNA was retransformed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3451. 
     EXAMPLES 28 
     Construction of pMON25185 
     pMON25185 was constructed using PCR to amplify a DNA fragment from pMON3451 as the template using the oligomers 96for.seg (SEQ ID NO:17) and 96rev.seq (SEQ ID NO:18). The oligomer 9697for.seq was designed to create six codon changes. The PCR reaction conditions were the same as described in Example 25, except 10 ng of pMON3456 plasmid DNA was added. The purified PCR product was ligated with the ˜3970 base pair NcoI/NheI pMON3456 vector fragment. Positive clones containing the AT-rich replacement insert were identified as described in Example 21. The resulting plasmid was designated pMON25185. The resulting gene, had 6 codon changes at the 5′ end of the gene. Plasmid, pMON25185 containing the gene sequence of (SEQ ID NO:39), DNA was retransformed into  E. coli  strain JM101 cells for protein expression. The protein expressed is the same as that expressed from pMON3456. 
     EXAMPLE 29 
     The G-CSF amino acid substitution variants of the present invention were made using PCR mutagenesis techniques as described in WO 94/12639 and WO 94/12638. These and other variants (i.e. amino acid substitutions, insertions or deletions and N-terminal or C-terminal extensions) could also be made, by one skilled in the art, using a variety of other methods including synthetic gene assembly or site-directed mutagenesis (see Taylor et al.,  Nucl. Acids Res.,  13:7864-8785, 1985; Kunkel et al.,  Proc. Natl. Acad. Sci. USA,  82:488-492, 1985; Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989, WO 94/12639 and WO 94/12638). These substitutions can be made one at a time or in combination with other amino acid substitutions, and/or deletions, and/or insertions and/or extensions. After sequence verification of the changes, the plasmid DNA can be transfected into an appropriate mammalian cell, insect cell or bacterial strain such as  E. coli  for production. Known variants of G-CSF, which are active, include substitutions at positions 1 (Thr to Ser, Arg or Gly, 2 (Pro to Leu), 3 (Leu to Arg or Ser) and 17 (Cys to Ser) and deletions of amino acids 1-11 (Kuga et al.  Biochemicla and Biophysical Research Comm.  159:103-111, 1989). It is understood that these G-CSF amino acid substitution variants could serve as the template sequence for the rearrangement of the amino acid sequence as described in the other examples. 
     Bioactivity determination of G-CSF amino acid substitution variants. 
     The G-CSF amino acid substitution variants were assayed in the Baf/3 cell line, transfected with the human G-CSF receptor, proliferation assay to determine their bioactivity relative to native G-CSF. The G-CSF variants tested and their relative bioactivity are shown in Table 5. A “+” indicates that the activity was comparable to native G-CSF and “−” indicates that the activity was significantly decreased or not detected. 
     
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 CELL PROLIFERATION ACTIVITY OF G-CSF 
               
               
                 VARIANTS IN BAF/3 CELL LINE TRANSFECTED 
               
               
                 WITH THE HUMAN G-CSF RECEPTOR 
               
             
          
           
               
                 aa position 
                 native aa 
                 mutant aa 
                 activity * 
               
               
                   
               
             
          
           
               
                 13 
                 Phe 
                 Ser 
                 + 
               
               
                 13 
                 Phe 
                 His 
                 + 
               
               
                 13 
                 Phe 
                 Thr 
                 + 
               
               
                 13 
                 Phe 
                 Pro 
                 + 
               
               
                 16 
                 Lys 
                 Pro 
                 + 
               
               
                 16 
                 Lys 
                 Ser 
                 + 
               
               
                 16 
                 Lys 
                 Thr 
                 + 
               
               
                 16 
                 Lys 
                 His 
                 + 
               
               
                 18 
                 Leu 
                 Pro 
                 + 
               
               
                 18 
                 Leu 
                 His 
                 + 
               
               
                 18 
                 Leu 
                 Cys 
                 + 
               
               
                 18 
                 Leu 
                 Ile 
                 + 
               
               
                 19 
                 Glu 
                 Ala 
                 − 
               
               
                 19 
                 Glu 
                 Thr 
                 − 
               
               
                 19 
                 Glu 
                 Arg 
                 − 
               
               
                 19 
                 Glu 
                 Pro 
                 − 
               
               
                 19 
                 Glu 
                 Leu 
                 − 
               
               
                 19 
                 Glu 
                 Gly 
                 − 
               
               
                 19 
                 Glu 
                 Ser 
                 − 
               
               
                 22 
                 Arg 
                 Tyr 
                 + 
               
               
                 22 
                 Arg 
                 Ser 
                 + 
               
               
                 22 
                 Arg 
                 Ala 
                 + 
               
               
                 22 
                 Arg 
                 Val 
                 + 
               
               
                 22 
                 Arg 
                 Thr 
                 + 
               
               
                 24 
                 Ile 
                 Pro 
                 + 
               
               
                 24 
                 Ile 
                 Leu 
                 + 
               
               
                 24 
                 Ile 
                 Tyr 
                 + 
               
               
                 27 
                 Asp 
                 Gly 
                 + 
               
               
                 30 
                 Ala 
                 Ile 
                 + 
               
               
                 30 
                 Ala 
                 Leu 
                 + 
               
               
                 34 
                 Lys 
                 Ser 
                 + 
               
               
                 43 
                 His 
                 Gly 
                 + 
               
               
                 43 
                 His 
                 Thr 
                 + 
               
               
                 43 
                 His 
                 Val 
                 + 
               
               
                 43 
                 His 
                 Lys 
                 + 
               
               
                 43 
                 His 
                 Trp 
                 + 
               
               
                 43 
                 His 
                 Ala 
                 + 
               
               
                 43 
                 His 
                 Arg 
                 + 
               
               
                 43 
                 His 
                 Cys 
                 + 
               
               
                 43 
                 His 
                 Leu 
                 + 
               
               
                 44 
                 Pro 
                 Arg 
                 + 
               
               
                 44 
                 Pro 
                 Asp 
                 + 
               
               
                 44 
                 Pro 
                 Val 
                 + 
               
               
                 44 
                 Pro 
                 Ala 
                 + 
               
               
                 44 
                 Pro 
                 His 
                 + 
               
               
                 44 
                 Pro 
                 Gln 
                 + 
               
               
                 44 
                 Pro 
                 Trp 
                 + 
               
               
                 44 
                 Pro 
                 Gly 
                 + 
               
               
                 44 
                 Pro 
                 Thr 
                 + 
               
               
                 46 
                 Glu 
                 Ala 
                 + 
               
               
                 46 
                 Glu 
                 Arg 
                 + 
               
               
                 46 
                 Glu 
                 Phe 
                 + 
               
               
                 46 
                 Glu 
                 Ile 
                 + 
               
               
                 47 
                 Leu 
                 Thr 
                 + 
               
               
                 49 
                 Leu 
                 Phe 
                 + 
               
               
                 49 
                 Leu 
                 Arg 
                 + 
               
               
                 49 
                 Leu 
                 Ser 
                 + 
               
               
                 50 
                 Leu 
                 His 
                 + 
               
               
                 50 
                 Leu 
                 Pro 
                 + 
               
               
                 51 
                 Gly 
                 Ser 
                 + 
               
               
                 51 
                 Gly 
                 Met 
                 + 
               
               
                 54 
                 Leu 
                 His 
                 + 
               
               
                 67 
                 Gln 
                 Lys 
                 + 
               
               
                 67 
                 Gln 
                 Leu 
                 + 
               
               
                 67 
                 Gln 
                 Cys 
                 + 
               
               
                 67 
                 Gln 
                 Lys 
                 + 
               
               
                 70 
                 Gln 
                 Pro 
                 + 
               
               
                 70 
                 Gln 
                 Leu 
                 + 
               
               
                 70 
                 Gln 
                 Arg 
                 + 
               
               
                 70 
                 Gln 
                 Ser 
                 + 
               
               
                 104 
                 Asp 
                 Gly 
                 + 
               
               
                 104 
                 Asp 
                 Val 
                 + 
               
               
                 108 
                 Leu 
                 Ala 
                 + 
               
               
                 108 
                 Leu 
                 Val 
                 + 
               
               
                 108 
                 Leu 
                 Arg 
                 + 
               
               
                 108 
                 Leu 
                 Gly 
                 + 
               
               
                 108 
                 Leu 
                 Trp 
                 + 
               
               
                 108 
                 Leu 
                 Gln 
                 + 
               
               
                 115 
                 Thr 
                 His 
                 + 
               
               
                 115 
                 Thr 
                 Leu 
                 + 
               
               
                 115 
                 Thr 
                 Ala 
                 + 
               
               
                 115 
                 Thr 
                 Ile 
                 + 
               
               
                 120 
                 Gln 
                 Gly 
                 + 
               
               
                 120 
                 Gln 
                 Arg 
                 + 
               
               
                 120 
                 Gln 
                 Lys 
                 + 
               
               
                 120 
                 Gln 
                 His 
                 + 
               
               
                 123 
                 Glu 
                 Arg 
                 + 
               
               
                 123 
                 Glu 
                 Phe 
                 + 
               
               
                 123 
                 Glu 
                 Thr 
                 + 
               
               
                 144 
                 Phe 
                 His 
                 + 
               
               
                 144 
                 Phe 
                 Arg 
                 + 
               
               
                 144 
                 Phe 
                 Pro 
                 + 
               
               
                 144 
                 Phe 
                 Leu 
                 + 
               
               
                 144 
                 Phe 
                 Glu 
                 + 
               
               
                 146 
                 Arg 
                 Gln 
                 + 
               
               
                 147 
                 Arg 
                 Gln 
                 + 
               
               
                 156 
                 His 
                 Asp 
                 − 
               
               
                 156 
                 His 
                 Ser 
                 + 
               
               
                 156 
                 His 
                 Gly 
                 + 
               
               
                 159 
                 Ser 
                 Arg 
                 + 
               
               
                 159 
                 Ser 
                 Thr 
                 + 
               
               
                 159 
                 Ser 
                 Tyr 
                 + 
               
               
                 159 
                 Ser 
                 Tyr 
                 + 
               
               
                 162 
                 Glu 
                 Gly 
                 − 
               
               
                 162 
                 Glu 
                 Trp 
                 + 
               
               
                 162 
                 Glu 
                 Leu 
                 + 
               
               
                 163 
                 Val 
                 Arg 
                 + 
               
               
                 163 
                 Val 
                 Arg 
                 + 
               
               
                 163 
                 Val 
                 Gly 
                 + 
               
               
                 165 
                 Tyr 
                 Cys 
                 not determined 
               
               
                 169 
                 Ser 
                 Leu 
                 + 
               
               
                 169 
                 Ser 
                 Cys 
                 + 
               
               
                 169 
                 Ser 
                 Arg 
                 + 
               
               
                 170 
                 His 
                 Arg 
                 + 
               
               
                 170 
                 His 
                 Ser 
                 + 
               
               
                   
               
             
          
         
       
     
     EXAMLPLE 30-37 
     Examples 30-37 were made in a similar manner as described in Example 6 using the plasmid pMON13037 as the template and the oligonucleotide primers indicated in Table 6. The resulting gene and the designated plasmid pMON # and the protein encoded are indicated in Table 6. 
     
       
         
               
               
               
               
               
             
           
               
                 TABLE 6 
               
               
                   
               
               
                   
                   
                   
                   
                 resulting 
               
               
                 Example 
                 breakpoint 
                 primers 
                 resulting gene 
                 protein 
               
               
                   
               
             
             
               
                 30 
                 48/49 
                 49start 
                 pMON3460 
                 (SEQ ID NO:95) 
               
               
                   
                   
                 (SEQ ID NO:68) 
                 (SEQ ID NO:86) 
                   
               
               
                   
                   
                 48stop 
                   
               
               
                   
                   
                 (SEQ ID NO:69) 
               
               
                 31 
                 76/77 
                 77start 
                 pMON3461 
                 (SEQ ID NO:96) 
               
               
                   
                   
                 (SEQ ID NO:70) 
                 (SEQ ID NO:87) 
               
               
                   
                 81/82 
                 76stop 
               
               
                   
                   
                 (SEQ ID NO:71) 
               
               
                 32 
                 81/82 
                 82start 
                 pMON3462 
                 (SEQ ID NO:97) 
               
               
                   
                   
                 (SEQ ID NO:72) 
                 (SEQ ID NO:88) 
               
               
                   
                   
                 81stop 
               
               
                   
                   
                 (SEQ ID NO:73) 
               
               
                 33 
                 83/84         84start 
                 pMON3463 
                 (SEQ ID NO:98) 
               
               
                   
                   
                 (SEQ ID NO:74) 
                 (SEQ ID NO:88) 
               
               
                   
                   
                 83stop 
               
               
                   
                   
                 (SEQ ID NO:75) 
               
               
                 34 
                 90/91 
                 91start 
                 pMON3464 
                 (SEQ ID NO:99) 
               
               
                   
                   
                 (SEQ ID NO:76) 
                 (SEQ ID NO:89) 
               
               
                   
                   
                 90stop 
               
               
                   
                   
                 (SEQ ID NO:77) 
               
               
                 35 
                 111/112 
                 112start 
                 pMON3465 
               
               
                   
                   
                 (SEQ ID NO:78) 
                 (SEQ ID NO:90) 
                 (SEQ ID NO:100) 
               
               
                   
                   
                 111stop 
               
               
                   
                   
                 (SEQ ID NO:79) 
               
               
                 36 
                 116/117 
                 117start 
                 pMON3466 
                 (SEQ ID NO:101) 
               
               
                   
                   
                 (SEQ ID NO:80) 
                 (SEQ ID NO:91) 
               
               
                   
                   
                 116stop 
               
               
                   
                   
                 (SEQ ID NO:81) 
               
               
                 37 
                 118/119 
                 119start 
                 pMON3467 
                 (SEQ ID NO:102) 
               
               
                   
                   
                 (SEQ ID NO:82) 
                 (SEQ ID NO:92) 
               
               
                   
                   
                 118stop 
               
               
                   
                   
                 (SEQ ID NO:83) 
               
               
                   
               
             
          
         
       
     
     The G-CSF receptor agonist genes in pMON3640, pMON3461, pMON3462, pMON3463, pMON3464, pMON3465, pMON3466 and pMON3467 were transferred to an  E. coli  expression vector, pMON2341, as an NcoI/HindIII restriction fragment, resulting in the plasmids pMON3468, pMON3469, pMON3470, pMON3471, pMON3472, pMON3473, pMON3474 and pMON3498 respectively. 
     EXAMPLE 38 
     The plasmid, pMON3468, resulted in low expression levels in  E. coli  of the desired G-CSF receptor agonist. The 5′ end of the gene was redesigned to use codon selection that was AT rich to increase expression levels. The oligonucleotides, Z4849AT.for (SEQ ID NO:84) and Z4849AT.rev (SEQ ID NO:85), were used to re-engineer the gene. The resulting plasmid, pMON3499, containing the gene (SEQ ID NO:94) encodes the G-CSF receptor agonist of (SEQ ID NO:103). 
     EXAMPLE 39 
     The G-CSF receptor agonists were assayed in the Baf/3 cell line, transfected with the human G-CSF receptor, (Baf/3-G-CSF) proliferation assay to determine their bioactivity relative to native G-CSF. The activity of the receptor agonists is shown in Table 7. 
     
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 G-CSF receptor agonist activity in 
               
               
                 Baf/3-G-CSF cell proliferation assay 
               
             
          
           
               
                   
                 break- 
                   
                 
                   E. coli 
                 
                   
               
               
                 PMON# 
                 point 
                 Expression  
                 refold 
                 EC50 (pM) 
               
               
                   
               
               
                 native G-CSF 
                   
                   
                   
                 60 pM 
               
               
                 pMON25182 
                 125/126 
                 + 
                 + 
                 38 pM 
               
               
                 pMON25183 
                 132/133 
                 + 
                 + 
                 58 pM 
               
               
                 pMON25184 
                 141/142 
                 + 
                 + 
                 70 pM 
               
               
                 pMON25186 
                 125/126 
                 + 
                 + 
                 92 pM 
               
               
                 pMON25187 
                 132/133 
                 + 
                 + 
                 83 pM 
               
               
                 pMON25188 
                 141/142 
                 + 
                 + 
                 41 pM 
               
               
                 pMON3450 
                 38/39 
                 + 
                 + 
                 121 pM 
               
               
                 pMON3455 
                 38/39 
                 + 
                 + 
                 102 pM 
               
               
                 pMON3499 
                 48/49 
                 + 
                 + 
                 137 pM 
               
               
                 pMON3470 
                 81/82 
                 + 
                 + 
                 no 
               
               
                   
                   
                   
                   
                 activity 
               
               
                   
                   
                   
                   
                 detected 
               
               
                 pMON3473 
                 111/112 
                 + 
                 − 
               
               
                   
               
             
          
         
       
     
     EXAMPLES 40-52 
     The plasmids in Table 8 contain genes encoding sequence rearranged G-CSF receptor agonists that were made by the method of Horlich et al (Protein Eng. 5:427-431, 1992). As described in Materials and Methods, the tandem repeat of the G-CSF Ser 17  gene was maintained on a pACYC177 based plasmid (Chang and Cohen,  J. Bacteriol.  1341141-1156, 1978), containing the sequence; GAG ATG GCT, encoding; Asp Met Ala, following immediately downstream of amino acid 174 of the first copy of the G-CSF Ser 17  gene and immediately preceeding amino acid 1 of the second copy of the G-CSF Ser 17  gene. The resulting sequence rearranged G-CSF receptor agonists have the linker; Asp Met Ala, between the original C-terminus and original N-terminus of G-CSF Ser 17 . The sequence rearranged G-CSF receptor agonists encoded by the plasmids of Table 8 were identified using a G-CSF receptor binding screen (Wantanabe et al.  Analyt. Biochem  195:38-44, 1991). The sequence rearranged G-CSF receptor agonists shown in Table 8 had receptor binding comparable to or better than native recombinant hG-CSF. 
     
       
         
               
               
               
               
             
           
               
                 TABLE 8 
               
               
                   
               
               
                 plasmid 
                   
                   
                 protein 
               
               
                 designation 
                 breakpoint 
                 gene sequence 
                 sequence 
               
               
                   
               
             
             
               
                 pG32 
                 2-3 
                 SEQ ID NO:110 
                 SEQ ID NO:122 
               
               
                 pG1110 
                 10-11 
                 SEQ ID NO: 104 
                 SEQ ID NO: 116 
               
               
                 pG1312 
                 12-13 
                 SEQ ID NO: 107 
                 SEQ ID NO: 119 
               
               
                 pG4948 
                 48-49 
                 SEQ ID NO: 111 
                 SEQ ID NO: 123 
               
               
                 pG5960 
                 59-60 
                 SEQ ID NO: 112 
                 SEQ ID NO: 124 
               
               
                 pG6667 
                 66-67 
                 SEQ ID NO: 113 
                 SEQ ID NO: 125 
               
               
                 pG6869 
                 68-69 
                 SEQ ID NO: 114 
                 SEQ ID NO: 126 
               
               
                 pG123122 
                 122-123 
                 SEQ ID NO: 105 
                 SEQ ID NO: 117 
               
               
                 pG159158 
                 158-159 
                 SEQ ID NO: 108 
                 SEQ ID NO: 120 
               
               
                 pG7170 
                 70-71 
                 SEQ ID NO: 115 
                 SEQ ID NO: 127 
               
               
                 pG125124 
                 124-125 
                 SEQ ID NO: 106 
                 SEQ ID NO: 118 
               
               
                 pG1918 
                 18-19 
                 SEQ ID NO: 109 
                 SEQ ID NO: 121 
               
               
                 pG170169 
                 169-170 
                 SEQ ID NO: 129 
                 SEQ ID NO: 128 
               
               
                   
               
             
          
         
       
     
     Additional techniques for the construction of the variant genes, recombinant protein expression, protein purification, protein characterization, biological activity determination can be found in WO 94/12639, WO 94/12638, WO 95/20976, WO 95/21197, WO 95/20977, WO 95/21254 which are hereby incorporated by reference in their entirety. 
     All references, patents or applications cited herein are incorporated by reference in their entirety as if written herein. 
     Various other examples will be apparent to the person skilled in the art after reading the present disclosure without departing from the spirit and scope of the invention. It is intended that all such other examples be included within the scope of the appended claims. 
     
       
         
           
             129 
           
           
             
               174 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               protein 
             
             
               Modified-site 
                /note= “Xaa at position 1 is Thr,
               Ser, Arg, Tyr or Gly;”
 
             
             
               Modified-site 
                /note= “Xaa at position 2 is Pro or
               Leu;”
 
             
             
               Modified-site 
                /note= “Xaa at position 3 is Leu,
               Arg, Tyr or Ser;”
 
             
             
               Modified-site 
                13 
                /note= “Xaa at position 13 is Phe,
               Ser, His, Thr or Pro;”
 
             
             
               Modified-site 
                16 
                /note= “Xaa at position 16 is Lys,
               Pro, Ser, thr or His;”
 
             
             
               Modified-site 
                17 
                /note= “Xaa at position 17 is Cys,
               Ser, Gly, Ala, Ile, Tyr or Arg;”
 
             
             
               Modified-site 
                18 
                /note= “Xaa at position 18 is Leu,
               Thr, Pro, His, Ile or Cys;”
 
             
             
               Modified-site 
                22 
                /note= “Xaa at position 22 is Arg,
               Tyr, Ser, Thr or Ala;”
 
             
             
               Modified-site 
                24 
                /note= “Xaa at position 24 is Ile,
               Pro, Tyr or Leu;”
 
             
             
               Modified-site 
                27 
                /note= “Xaa at position 27 is Asp,
               or Gly;”
 
             
             
               Modified-site 
                30 
                /note= “Xaa at position 30 is Ala,
               Ile, Leu or Gly;”
 
             
             
               Modified-site 
                34 
                /note= “Xaa at position 34 is Lys
               or Ser;”
 
             
             
               Modified-site 
                36 
                /note= “Xaa at position 36 is Cys
               or Ser;”
 
             
             
               Modified-site 
                42 
                /note= “Xaa at position 42 is Cys
               or Ser;”
 
             
             
               Modified-site 
                43 
                /note= “Xaa at position 43 is His,
               Thr, Gly, Val, Lys, Trp, Ala, Arg, Cys, or Leu;”
 
             
             
               Modified-site 
                44 
                /note= “Xaa at position 44 is Pro,
               Gly, Arg, Asp, Val, Ala, His, Trp, Gln, or Thr;”
 
             
             
               Modified-site 
                46 
                /note= “Xaa at position 46 is Glu,
               Arg, Phe, Arg, Ile or Ala;”
 
             
             
               Modified-site 
                47 
                /note= “Xaa at position 47 is Leu
               or Thr;”
 
             
             
               Modified-site 
                49 
                /note= “Xaa at position 49 is Leu,
               Phe, Arg or Ser;”
 
             
             
               Modified-site 
                50 
                /note= “Xaa at position 50 is Leu,
               Ile, His, Pro or Tyr;”
 
             
             
               Modified-site 
                54 
                /note= “Xaa at position 54 is Leu
               or His;”
 
             
             
               Modified-site 
                64 
                /note= “Xaa at position 64 is Cys
               or Ser;”
 
             
             
               Modified-site 
                67 
                /note= “Xaa at position 67 is Gln,
               Lys, Leu or Cys;”
 
             
             
               Modified-site 
                70 
                /note= “Xaa at position 70 is Gln,
               Pro, Leu, Arg or Ser;”
 
             
             
               Modified-site 
                74 
                /note= “Xaa at position 74 is Cys
               or Ser;”
 
             
             
               Modified-site 
                104 
                /note= “Xaa at position 104 is Asp,
               Gly or Val;”
 
             
             
               Modified-site 
                108 
                /note= “Xaa at position 108 is Leu,
               Ala, Val, Arg, Trp, Gln or Gly;”
 
             
             
               Modified-site 
                115 
                /note= “Xaa at position 115 is Thr,
               His, Leu or Ala;”
 
             
             
               Modified-site 
                120 
                /note= “Xaa at position 120 is Gln,
               Gly, Arg, Lys or His”
 
             
             
               Modified-site 
                123 
                /note= “Xaa at position 123 is Glu,
               Arg, Phe or Thr”
 
             
             
               Modified-site 
                144 
                /note= “Xaa at position 144 is Phe,
               His, Arg, Pro, Leu, Gln or Glu;”
 
             
             
               Modified-site 
                146 
                /note= “Xaa at position 146 is Arg
               or Gln;”
 
             
             
               Modified-site 
                147 
                /note= “Xaa at position 147 is Arg
               or Gln;”
 
             
             
               Modified-site 
                156 
                /note= “Xaa at position 156 is His,
               Gly or Ser;”
 
             
             
               Modified-site 
                159 
                /note= “Xaa at position 159 is Ser,
               Arg, Thr, Tyr, Val or Gly;”
 
             
             
               Modified-site 
                162 
                /note= “Xaa at position 162 is Glu,
               Leu, Gly or Trp;”
 
             
             
               Modified-site 
                163 
                /note= “Xaa at position 163 is Val,
               Gly, Arg or Ala;”
 
             
             
               Modified-site 
                169 
                /note= “Xaa at position 169 is Arg,
               Ser, Leu, Arg or Cys;”
 
             
             
               Modified-site 
                170 
                /note= “Xaa at position 170 is His,
               Arg or Ser;”
 
             
             1
Xaa Xaa Xaa Gly Pro Ala Ser Ser Leu Pro Gln Ser Xaa Leu Leu Xaa
1               5                   10                  15
Xaa Xaa Glu Gln Val Xaa Lys Xaa Gln Gly Xaa Gly Ala Xaa Leu Gln
            20                  25                  30
Glu Xaa Leu Xaa Ala Thr Tyr Lys Leu Xaa Xaa Xaa Glu Xaa Xaa Val
        35                  40                  45
Xaa Xaa Gly His Ser Xaa Gly Ile Pro Trp Ala Pro Leu Ser Ser Xaa
    50                  55                  60
Pro Ser Xaa Ala Leu Xaa Leu Ala Gly Xaa Leu Ser Gln Leu His Ser
65                  70                  75                  80
Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
                85                  90                  95
Pro Glu Leu Gly Pro Thr Leu Xaa Thr Leu Gln Xaa Asp Val Ala Asp
            100                 105                 110
Phe Ala Xaa Thr Ile Trp Gln Xaa Met Glu Xaa Xaa Gly Met Ala Pro
        115                 120                 125
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Xaa
    130                 135                 140
Gln Xaa Xaa Ala Gly Gly Val Leu Val Ala Ser Xaa Leu Gln Xaa Phe
145                 150                 155                 160
Leu Xaa Xaa Ser Tyr Arg Val Leu Xaa Xaa Leu Ala Gln Pro
                165                 170
 
           
           
             
               4 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             2
Gly Gly Gly Ser
1
 
           
           
             
               54 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             3
GCTCTGAGAG CCGCCAGAGC CGCCAGAGGG CTGCGCAAGG TGGCGTAGAA CGCG           54
 
           
           
             
               54 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             4
CAGCCCTCTG GCGGCTCTGG CGGCTCTCAG AGCTTCCTGC TCAAGTCTTT AGAG           54
 
           
           
             
               18 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             5
GGGCTGCGCA AGGTGGCG                                                   18
 
           
           
             
               21 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             6
ACACCATTGG GCCCTGCCAG C                                               21
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             7
GATCGACCAT GGCTTACAAG CTGTGCCACC CC                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             8
CGATCGAAGC TTATTAGGTG GCACACAGCT TCTCCT                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             9
GATCGACCAT GGCTCCCGAG TTGGGTCCCA CC                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             10
CGATCGAAGC TTATTAGGAT ATCCCTTCCA GGGCCT                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             11
GATCGACCAT GGCTATGGCC CCTGCCCTGC AG                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             12
CGATCGAAGC TTATTATCCC AGTTCTTCCA TCTGCT                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             13
GATCGACCAT GGCTACCCAG GGTGCCATGC CG                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             14
CGATCGAAGC TTATTAGGGC TGCAGGGCAG GGGCCA                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             15
GATCGACCAT GGCTTCTGCT TTCCAGCGCC GG                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             16
CGATCGAAGC TTATTAGGCG AAGGCCGGCA TGGCAC                               36
 
           
           
             
               33 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             17
ATATCCATGG CTCCGGAACT GGGTCCAACT CTG                                  33
 
           
           
             
               24 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             18
ACCTCCAGGA AGCTCTGCAG ATGG                                            24
 
           
           
             
               65 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             19
TATATCCATG GCTATGGCTC CAGCTCTGCA ACCAACTCAA GGTGCAATGC CAGCATTTGC     60
ATCTG                                                                 65
 
           
           
             
               63 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             20
GATGGCTAGC AACCAGAACA CCACCTGCAC GACGTTGAAA AGCAGATGCA AATGCTGGCA     60
TTG                                                                   63
 
           
           
             
               57 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             21
TATATCCATG GCTACTCAAG GTGCTATGCC AGCTTTTGCT TCTGCTTTTC AACGTCG        57
 
           
           
             
               58 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             22
GCAGATGGCT AGCAACCAGA ACACCACCTG CACGACGTTG AAAAGCAGAA GCAAAAGC       58
 
           
           
             
               44 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             23
CATGGCTTCT GCTTTTCAAC GTCGTGCAGG TGGTGTTCTG GTTG                      44
 
           
           
             
               44 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             24
CTAGCAACCA GAACACCACC TGCACGACGT TGAAAAGCAG AAGC                      44
 
           
           
             
               525 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             25
ATGGCTTACA AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC TCTGGGCATC     60
CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC    120
CAACTCCATA GCGGCCTTTT CCTCTACCAG GGGCTCCTGC AGGCCCTGGA AGGGATATCC    180
CCCGAGTTGG GTCCCACCTT GGACACACTG CAGCTGGACG TCGCCGACTT TGCCACCACC    240
ATCTGGCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC    300
ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT    360
CTGCAGAGCT TCCTGGAGGT GTCGTACCGC GTTCTACGCC ACCTTGCGCA GCCCTCTGGC    420
GGCTCTGGCG GCTCTCAGAG CTTCCTGCTC AAGTCTTTAG AGCAAGTGAG GAAGATCCAG    480
GGCGATGGCG CAGCGCTCCA GGAGAAGCTG TGTGCCACCT AATAA                    525
 
           
           
             
               525 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             26
ATGGCTCCCG AGTTGGGTCC CACCTTGGAC ACACTGCAGC TGGACGTCGC CGACTTTGCC     60
ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC CTGCCCTGCA GCCCACCCAG    120
GGTGCCATGC CGGCCTTCGC CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT    180
AGCCATCTGC AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC    240
TCTGGCGGCT CTGGCGGCTC TCAGAGCTTC CTGCTCAAGT CTTTAGAGCA AGTGAGGAAG    300
ATCCAGGGCG ATGGCGCAGC GCTCCAGGAG AAGCTGTGTG CCACCTACAA GCTGTGCCAC    360
CCCGAGGAGC TGGTGCTGCT CGGACACTCT CTGGGCATCC CCTGGGCTCC CCTGAGCTCC    420
TGCCCCAGCC AGGCCCTGCA GCTGGCAGGC TGCTTGAGCC AACTCCATAG CGGCCTTTTC    480
CTCTACCAGG GGCTCCTGCA GGCCCTGGAA GGGATATCCT AATAA                    525
 
           
           
             
               525 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             27
ATGGCTATGG CCCCTGCCCT GCAGCCCACC CAGGGTGCCA TGCCGGCCTT CGCCTCTGCT     60
TTCCAGCGCC GGGCAGGAGG GGTCCTGGTT GCTAGCCATC TGCAGAGCTT CCTGGAGGTG    120
TCGTACCGCG TTCTACGCCA CCTTGCGCAG CCCTCTGGCG GCTCTGGCGG CTCTCAGAGC    180
TTCCTGCTCA AGTCTTTAGA GCAAGTGAGG AAGATCCAGG GCGATGGCGC AGCGCTCCAG    240
GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT GCTCGGACAC    300
TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA    360
GGCTGCTTGA GCCAACTCCA TAGCGGCCTT TTCCTCTACC AGGGGCTCCT GCAGGCCCTG    420
GAAGGGATAT CCCCCGAGTT GGGTCCCACC TTGGACACAC TGCAGCTGGA CGTCGCCGAC    480
TTTGCCACCA CCATCTGGCA GCAGATGGAA GAACTGGGAT AATAA                    525
 
           
           
             
               525 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             28
ATGGCTACCC AGGGTGCCAT GCCGGCCTTC GCCTCTGCTT TCCAGCGCCG GGCAGGAGGG     60
GTCCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT CGTACCGCGT TCTACGCCAC    120
CTTGCGCAGC CCTCTGGCGG CTCTGGCGGC TCTCAGAGCT TCCTGCTCAA GTCTTTAGAG    180
CAAGTGAGGA AGATCCAGGG CGATGGCGCA GCGCTCCAGG AGAAGCTGTG TGCCACCTAC    240
AAGCTGTGCC ACCCCGAGGA GCTGGTGCTG CTCGGACACT CTCTGGGCAT CCCCTGGGCT    300
CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG CCAACTCCAT    360
AGCGGCCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG AAGGGATATC CCCCGAGTTG    420
GGTCCCACCT TGGACACACT GCAGCTGGAC GTCGCCGACT TTGCCACCAC CATCTGGCAG    480
CAGATGGAAG AACTGGGAAT GGCCCCTGCC CTGCAGCCCT AATAA                    525
 
           
           
             
               525 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             29
ATGGCTTCTG CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC     60
TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCTCTGG CGGCTCTGGC    120
GGCTCTCAGA GCTTCCTGCT CAAGTCTTTA GAGCAAGTGA GGAAGATCCA GGGCGATGGC    180
GCAGCGCTCC AGGAGAAGCT GTGTGCCACC TACAAGCTGT GCCACCCCGA GGAGCTGGTG    240
CTGCTCGGAC ACTCTCTGGG CATCCCCTGG GCTCCCCTGA GCTCCTGCCC CAGCCAGGCC    300
CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA CCAGGGGCTC    360
CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA CCTTGGACAC ACTGCAGCTG    420
GACGTCGCCG ACTTTGCCAC CACCATCTGG CAGCAGATGG AAGAACTGGG AATGGCCCCT    480
GCCCTGCAGC CCACCCAGGG TGCCATGCCG GCCTTCGCCT AATAA                    525
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             30
ATGGCTTACA AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC TCTGGGCATC     60
CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC    120
CAACTCCATA GCGGCCTTTT CCTCTACCAG GGGCTCCTGC AGGCCCTGGA AGGGATATCC    180
CCCGAGTTGG GTCCCACCTT GGACACACTG CAGCTGGACG TCGCCGACTT TGCCACCACC    240
ATCTGGCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC    300
ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT    360
CTGCAGAGCT TCCTGGAGGT GTCGTACCGC GTTCTACGCC ACCTTGCGCA GCCCACACCA    420
TTGGGCCCTG CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA    480
AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             31
ATGGCTCCCG AGTTGGGTCC CACCTTGGAC ACACTGCAGC TGGACGTCGC CGACTTTGCC     60
ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC CTGCCCTGCA GCCCACCCAG    120
GGTGCCATGC CGGCCTTCGC CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT    180
AGCCATCTGC AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC    240
ACACCATTGG GCCCTGCCAG CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA    300
GTGAGAAAGA TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC CACCTACAAG    360
CTGTGCCACC CCGAGGAGCT GGTGCTGCTC GGACACTCTC TGGGCATCCC CTGGGCTCCC    420
CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG CTGGCAGGCT GCTTGAGCCA ACTCCATAGC    480
GGCCTTTTCC TCTACCAGGG GCTCCTGCAG GCCCTGGAAG GGATATCCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (Synthetic)” 
             
             32
ATGGCTATGG CCCCTGCCCT GCAGCCCACC CAGGGTGCCA TGCCGGCCTT CGCCTCTGCT     60
TTCCAGCGCC GGGCAGGAGG GGTCCTGGTT GCTAGCCATC TGCAGAGCTT CCTGGAGGTG    120
TCGTACCGCG TTCTACGCCA CCTTGCGCAG CCCACACCAT TGGGCCCTGC CAGCTCCCTG    180
CCCCAGAGCT TCCTGCTCAA GTCTTTAGAG CAAGTGAGAA AGATCCAGGG CGATGGCGCA    240
GCGCTCCAGG AGAAGCTGTG TGCCACCTAC AAGCTGTGCC ACCCCGAGGA GCTGGTGCTG    300
CTCGGACACT CTCTGGGCAT CCCCTGGGCT CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG    360
CAGCTGGCAG GCTGCTTGAG CCAACTCCAT AGCGGCCTTT TCCTCTACCA GGGGCTCCTG    420
CAGGCCCTGG AAGGGATATC CCCCGAGTTG GGTCCCACCT TGGACACACT GCAGCTGGAC    480
GTCGCCGACT TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGATA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             33
ATGGCTACCC AGGGTGCCAT GCCGGCCTTC GCCTCTGCTT TCCAGCGCCG GGCAGGAGGG     60
GTCCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT CGTACCGCGT TCTACGCCAC    120
CTTGCGCAGC CCACACCATT GGGCCCTGCC AGCTCCCTGC CCCAGAGCTT CCTGCTCAAG    180
TCTTTAGAGC AAGTGAGAAA GATCCAGGGC GATGGCGCAG CGCTCCAGGA GAAGCTGTGT    240
GCCACCTACA AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC TCTGGGCATC    300
CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC    360
CAACTCCATA GCGGCCTTTT CCTCTACCAG GGGCTCCTGC AGGCCCTGGA AGGGATATCC    420
CCCGAGTTGG GTCCCACCTT GGACACACTG CAGCTGGACG TCGCCGACTT TGCCACCACC    480
ATCTGGCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             34
ATGGCTTCTG CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC     60
TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCACACC ATTGGGCCCT    120
GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC AAGTCTTTAG AGCAAGTGAG AAAGATCCAG    180
GGCGATGGCG CAGCGCTCCA GGAGAAGCTG TGTGCCACCT ACAAGCTGTG CCACCCCGAG    240
GAGCTGGTGC TGCTCGGACA CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC    300
AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG AGCCAACTCC ATAGCGGCCT TTTCCTCTAC    360
CAGGGGCTCC TGCAGGCCCT GGAAGGGATA TCCCCCGAGT TGGGTCCCAC CTTGGACACA    420
CTGCAGCTGG ACGTCGCCGA CTTTGCCACC ACCATCTGGC AGCAGATGGA AGAACTGGGA    480
ATGGCCCCTG CCCTGCAGCC CACCCAGGGT GCCATGCCGG CCTTCGCCTA ATAA          534
 
           
           
             
               531 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             35
ATGGCTCCGG AACTGGGTCC AACTCTGGAC ACACTGCAGC TGGACGTCGC CGACTTTGCC     60
ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC CTGCCCTGCA GCCCACCCAG    120
GGTGCCATGC CGGCCTTCGC CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT    180
AGCCATCTGC AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC    240
ACACCATTGG GCCCTGCCAG CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA    300
GTGAGAAAGA TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC CACCTACAAG    360
CTGTGCCACC CCGAGGAGCT GGTGCTGCTC GGACACTCTC TGGGCATCCC CTGGGCTCCC    420
CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG CTGGCAGGCT GCTTGAGCCA ACTCCATAGC    480
GGCCTTTTCC TCTACCAGGG GCTCCTGCAG GCCCTGGAAG GGATATCCTA A             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             36
ATGGCTATGG CTCCAGCTCT GCAACCAACT CAAGGTGCAA TGCCAGCATT TGCATCTGCT     60
TTTCAACGTC GTGCAGGTGG TGTTCTGGTT GCTAGCCATC TGCAGAGCTT CCTGGAGGTG    120
TCGTACCGCG TTCTACGCCA CCTTGCGCAG CCCACACCAT TGGGCCCTGC CAGCTCCCTG    180
CCCCAGAGCT TCCTGCTCAA GTCTTTAGAG CAAGTGAGAA AGATCCAGGG CGATGGCGCA    240
GCGCTCCAGG AGAAGCTGTG TGCCACCTAC AAGCTGTGCC ACCCCGAGGA GCTGGTGCTG    300
CTCGGACACT CTCTGGGCAT CCCCTGGGCT CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG    360
CAGCTGGCAG GCTGCTTGAG CCAACTCCAT AGCGGCCTTT TCCTCTACCA GGGGCTCCTG    420
CAGGCCCTGG AAGGGATATC CCCCGAGTTG GGTCCCACCT TGGACACACT GCAGCTGGAC    480
GTCGCCGACT TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGATA A             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             37
ATGGCTACTC AAGGTGCTAT GCCAGCTTTT GCTTCTGCTT TTCAACGTCG TGCAGGTGGT     60
GTTCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT CGTACCGCGT TCTACGCCAC    120
CTTGCGCAGC CCACACCATT GGGCCCTGCC AGCTCCCTGC CCCAGAGCTT CCTGCTCAAG    180
TCTTTAGAGC AAGTGAGAAA GATCCAGGGC GATGGCGCAG CGCTCCAGGA GAAGCTGTGT    240
GCCACCTACA AGCTGTGCCA CCCCGAGGAG CTGGTGCTGC TCGGACACTC TCTGGGCATC    300
CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC    360
CAACTCCATA GCGGCCTTTT CCTCTACCAG GGGCTCCTGC AGGCCCTGGA AGGGATATCC    420
CCCGAGTTGG GTCCCACCTT GGACACACTG CAGCTGGACG TCGCCGACTT TGCCACCACC    480
ATCTGGCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCTA A             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             38
ATGGCTTCTG CTTTTCAACG TCGTGCAGGT GGTGTTCTGG TTGCTAGCCA TCTGCAGAGC     60
TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCACACC ATTGGGCCCT    120
GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC AAGTCTTTAG AGCAAGTGAG AAAGATCCAG    180
GGCGATGGCG CAGCGCTCCA GGAGAAGCTG TGTGCCACCT ACAAGCTGTG CCACCCCGAG    240
GAGCTGGTGC TGCTCGGACA CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC    300
AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG AGCCAACTCC ATAGCGGCCT TTTCCTCTAC    360
CAGGGGCTCC TGCAGGCCCT GGAAGGGATA TCCCCCGAGT TGGGTCCCAC CTTGGACACA    420
CTGCAGCTGG ACGTCGCCGA CTTTGCCACC ACCATCTGGC AGCAGATGGA AGAACTGGGA    480
ATGGCCCCTG CCCTGCAGCC CACCCAGGGT GCCATGCCGG CCTTCGCCTA A             531
 
           
           
             
               522 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             39
ATGGCTCCGG AACTGGGTCC AACTCTGGAC ACACTGCAGC TGGACGTCGC CGACTTTGCC     60
ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC CTGCCCTGCA GCCCACCCAG    120
GGTGCCATGC CGGCCTTCGC CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT    180
AGCCATCTGC AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC    240
TCTGGCGGCT CTGGCGGCTC TCAGAGCTTC CTGCTCAAGT CTTTAGAGCA AGTGAGAAAG    300
ATCCAGGGCG ATGGCGCAGC GCTCCAGGAG AAGCTGTGTG CCACCTACAA GCTGTGCCAC    360
CCCGAGGAGC TGGTGCTGCT CGGACACTCT CTGGGCATCC CCTGGGCTCC CCTGAGCTCC    420
TGCCCCAGCC AGGCCCTGCA GCTGGCAGGC TGCTTGAGCC AACTCCATAG CGGCCTTTTC    480
CTCTACCAGG GGCTCCTGCA GGCCCTGGAA GGGATATCCT AA                       522
 
           
           
             
               522 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             40
ATGGCTATGG CTCCAGCTCT GCAACCAACT CAAGGTGCAA TGCCAGCATT TGCATCTGCT     60
TTTCAACGTC GTGCAGGTGG TGTTCTGGTT GCTAGCCATC TGCAGAGCTT CCTGGAGGTG    120
TCGTACCGCG TTCTACGCCA CCTTGCGCAG CCCTCTGGCG GCTCTGGCGG CTCTCAGAGC    180
TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA AAGATCCAGG GCGATGGCGC AGCGCTCCAG    240
GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT GCTCGGACAC    300
TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA    360
GGCTGCTTGA GCCAACTCCA TAGCGGCCTT TTCCTCTACC AGGGGCTCCT GCAGGCCCTG    420
GAAGGGATAT CCCCCGAGTT GGGTCCCACC TTGGACACAC TGCAGCTGGA CGTCGCCGAC    480
TTTGCCACCA CCATCTGGCA GCAGATGGAA GAACTGGGAT AA                       522
 
           
           
             
               522 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             41
ATGGCTACTC AAGGTGCTAT GCCAGCTTTT GCTTCTGCTT TTCAACGTCG TGCAGGTGGT     60
GTTCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT CGTACCGCGT TCTACGCCAC    120
CTTGCGCAGC CCTCTGGCGG CTCTGGCGGC TCTCAGAGCT TCCTGCTCAA GTCTTTAGAG    180
CAAGTGAGAA AGATCCAGGG CGATGGCGCA GCGCTCCAGG AGAAGCTGTG TGCCACCTAC    240
AAGCTGTGCC ACCCCGAGGA GCTGGTGCTG CTCGGACACT CTCTGGGCAT CCCCTGGGCT    300
CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG CCAACTCCAT    360
AGCGGCCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG AAGGGATATC CCCCGAGTTG    420
GGTCCCACCT TGGACACACT GCAGCTGGAC GTCGCCGACT TTGCCACCAC CATCTGGCAG    480
CAGATGGAAG AACTGGGAAT GGCCCCTGCC CTGCAGCCCT AA                       522
 
           
           
             
               522 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             42
ATGGCTTCTG CTTTTCAACG TCGTGCAGGT GGTGTTCTGG TTGCTAGCCA TCTGCAGAGC     60
TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCTCTGG CGGCTCTGGC    120
GGCTCTCAGA GCTTCCTGCT CAAGTCTTTA GAGCAAGTGA GAAAGATCCA GGGCGATGGC    180
GCAGCGCTCC AGGAGAAGCT GTGTGCCACC TACAAGCTGT GCCACCCCGA GGAGCTGGTG    240
CTGCTCGGAC ACTCTCTGGG CATCCCCTGG GCTCCCCTGA GCTCCTGCCC CAGCCAGGCC    300
CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA CCAGGGGCTC    360
CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA CCTTGGACAC ACTGCAGCTG    420
GACGTCGCCG ACTTTGCCAC CACCATCTGG CAGCAGATGG AAGAACTGGG AATGGCCCCT    480
GCCCTGCAGC CCACCCAGGG TGCCATGCCG GCCTTCGCCT AA                       522
 
           
           
             
               171 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             43
Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu
1               5                   10                  15
Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln
            20                  25                  30
Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln
        35                  40                  45
Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr
    50                  55                  60
Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp
65                  70                  75                  80
Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln
                85                  90                  95
Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly
            100                 105                 110
Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg
        115                 120                 125
Val Leu Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly Ser Gln
    130                 135                 140
Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp
145                 150                 155                 160
Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr
                165                 170
 
           
           
             
               171 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             44
Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp
1               5                   10                  15
Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro
            20                  25                  30
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe
        35                  40                  45
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe
    50                  55                  60
Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Ser Gly
65                  70                  75                  80
Gly Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val
                85                  90                  95
Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala
            100                 105                 110
Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser
        115                 120                 125
Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu
    130                 135                 140
Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr
145                 150                 155                 160
Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
                165                 170
 
           
           
             
               171 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             45
Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala
1               5                   10                  15
Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser
            20                  25                  30
Tyr Arg Val Leu Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly
        35                  40                  45
Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln
    50                  55                  60
Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu
65                  70                  75                  80
Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro
                85                  90                  95
Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly
            100                 105                 110
Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu
        115                 120                 125
Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr
    130                 135                 140
Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met
145                 150                 155                 160
Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro
                165                 170
 
           
           
             
               118 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               protein 
             
             46
TGGAATAAAA AAGAGAGAAG GAAAAGGATA GAAGAAGGGG GGGGAAGGGA GAAAAGGCAA     60
TTCGGAGGTA ACGAAGAAGC GGTGGGAAGG GGTATGAAAA AAATTTGGTG GGTAAAAG      118
 
           
           
             
               171 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             47
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
1               5                   10                  15
Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln
            20                  25                  30
Pro Ser Gly Gly Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu
        35                  40                  45
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
    50                  55                  60
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu
65                  70                  75                  80
Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser
                85                  90                  95
Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu
            100                 105                 110
Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu
        115                 120                 125
Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala
    130                 135                 140
Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu
145                 150                 155                 160
Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             48
Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu
1               5                   10                  15
Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln
            20                  25                  30
Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln
        35                  40                  45
Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr
    50                  55                  60
Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp
65                  70                  75                  80
Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln
                85                  90                  95
Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly
            100                 105                 110
Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg
        115                 120                 125
Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser
    130                 135                 140
Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile
145                 150                 155                 160
Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             49
Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp
1               5                   10                  15
Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro
            20                  25                  30
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe
        35                  40                  45
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe
    50                  55                  60
Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro
65                  70                  75                  80
Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu
                85                  90                  95
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
            100                 105                 110
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu
        115                 120                 125
Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser
    130                 135                 140
Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu
145                 150                 155                 160
Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             50
Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
1               5                   10                  15
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
            20                  25                  30
Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln
        35                  40                  45
Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu
    50                  55                  60
Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu
65                  70                  75                  80
Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu
                85                  90                  95
Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser
            100                 105                 110
Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His
        115                 120                 125
Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile
    130                 135                 140
Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala
145                 150                 155                 160
Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             51
Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala
1               5                   10                  15
Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser
            20                  25                  30
Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala
        35                  40                  45
Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg
    50                  55                  60
Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr
65                  70                  75                  80
Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu
                85                  90                  95
Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln
            100                 105                 110
Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln
        115                 120                 125
Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr
    130                 135                 140
Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp
145                 150                 155                 160
Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             52
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
1               5                   10                  15
Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln
            20                  25                  30
Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu
        35                  40                  45
Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu
    50                  55                  60
Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu
65                  70                  75                  80
Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser
                85                  90                  95
Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His
            100                 105                 110
Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile
        115                 120                 125
Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala
    130                 135                 140
Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala
145                 150                 155                 160
Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             53
Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp
1               5                   10                  15
Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro
            20                  25                  30
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe
        35                  40                  45
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe
    50                  55                  60
Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro
65                  70                  75                  80
Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu
                85                  90                  95
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
            100                 105                 110
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu
        115                 120                 125
Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser
    130                 135                 140
Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu
145                 150                 155                 160
Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             54
Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
1               5                   10                  15
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
            20                  25                  30
Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln
        35                  40                  45
Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu
    50                  55                  60
Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu
65                  70                  75                  80
Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu
                85                  90                  95
Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser
            100                 105                 110
Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His
        115                 120                 125
Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile
    130                 135                 140
Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala
145                 150                 155                 160
Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             55
Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala
1               5                   10                  15
Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser
            20                  25                  30
Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala
        35                  40                  45
Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg
    50                  55                  60
Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr
65                  70                  75                  80
Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu
                85                  90                  95
Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln
            100                 105                 110
Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln
        115                 120                 125
Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr
    130                 135                 140
Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp
145                 150                 155                 160
Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             56
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
1               5                   10                  15
Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln
            20                  25                  30
Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu
        35                  40                  45
Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu
    50                  55                  60
Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu
65                  70                  75                  80
Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser
                85                  90                  95
Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His
            100                 105                 110
Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile
        115                 120                 125
Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala
    130                 135                 140
Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala
145                 150                 155                 160
Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
                165                 170
 
           
           
             
               171 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             57
Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp
1               5                   10                  15
Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro
            20                  25                  30
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe
        35                  40                  45
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe
    50                  55                  60
Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Ser Gly
65                  70                  75                  80
Gly Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val
                85                  90                  95
Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala
            100                 105                 110
Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser
        115                 120                 125
Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu
    130                 135                 140
Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr
145                 150                 155                 160
Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
                165                 170
 
           
           
             
               169 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             58
Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
1               5                   10                  15
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
            20                  25                  30
Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln
        35                  40                  45
Pro Ser Gly Gly Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu
    50                  55                  60
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
65                  70                  75                  80
Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His
                85                  90                  95
Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala
            100                 105                 110
Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu
        115                 120                 125
Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly
    130                 135                 140
Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr
145                 150                 155                 160
Ile Trp Gln Gln Met Glu Glu Leu Gly
                165
 
           
           
             
               171 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             59
Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala
1               5                   10                  15
Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser
            20                  25                  30
Tyr Arg Val Leu Arg His Leu Ala Gln Pro Ser Gly Gly Ser Gly Gly
        35                  40                  45
Ser Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln
    50                  55                  60
Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu
65                  70                  75                  80
Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro
                85                  90                  95
Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly
            100                 105                 110
Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu
        115                 120                 125
Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr
    130                 135                 140
Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met
145                 150                 155                 160
Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro
                165                 170
 
           
           
             
               171 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             60
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
1               5                   10                  15
Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln
            20                  25                  30
Pro Ser Gly Gly Ser Gly Gly Ser Gln Ser Phe Leu Leu Lys Ser Leu
        35                  40                  45
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
    50                  55                  60
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu
65                  70                  75                  80
Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser
                85                  90                  95
Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu
            100                 105                 110
Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu
        115                 120                 125
Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala
    130                 135                 140
Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu
145                 150                 155                 160
Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
                165                 170
 
           
           
             
               8 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             61
Gly Gly Gly Ser Gly Gly Gly Ser
1               5
 
           
           
             
               12 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             62
Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser
1               5                   10
 
           
           
             
               7 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             63
Ser Gly Gly Ser Gly Gly Ser
1               5
 
           
           
             
               5 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             64
Glu Phe Gly Asn Met
1               5
 
           
           
             
               6 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             65
Glu Phe Gly Gly Asn Met
1               5
 
           
           
             
               9 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             66
Glu Phe Gly Gly Asn Gly Gly Asn Met
1               5
 
           
           
             
               7 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             67
Gly Gly Ser Asp Met Ala Gly
1               5
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             68
GATCGACCAT GGCTCTGCTC GGACACTCTC TG                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             69
CGATCGAAGC TTATTACACC AGCTCCTCGG GGTGGC                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             70
GATCGACCAT GGCTCAACTC CATAGCGGCC TT                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             71
CGATCGAAGC TTATTAGCTC AAGCAGCCTG CCAGCT                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             72
GATCGACCAT GGCTCTTTTC CTCTACCAGG GG                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             73
CGATCGAAGC TTATTAGCCG CTATGGAGTT GGCTCA                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             74
GATCGACCAT GGCTCTCTAC CAGGGGCTCC TG                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             75
CGATCGAAGC TTATTAGAAA AGGCCGCTAT GGAGTT                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             76
GATCGACCAT GGCTGCCCTG GAAGGGATAT CC                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             77
CGATCGAAGC TTATTACTGC AGGAGCCCCT GGTAGA                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             78
GATCGACCAT GGCTGACTTT GCCACCACCA TC                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             79
CGATCGAAGC TTATTAGGCG ACGTCCAGCT GCAGTG                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             80
GATCGACCAT GGCTATCTGG CAGCAGATGG AA                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             81
CGATCGAAGC TTATTAGGTG GTGGCAAAGT CGGCGA                               36
 
           
           
             
               32 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             82
GATCGACCAT GGCTCAGCAG ATGGAAGAAC TG                                   32
 
           
           
             
               36 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             83
CGATCGAAGC TTATTACCAG ATGGTGGTGG CAAAGT                               36
 
           
           
             
               50 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             84
CATGGCTTTG TTAGGACATT CTTTAGGTAT TCCATGGGCT CCTCTGAGCT                50
 
           
           
             
               40 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             85
CAGAGGAGCC CATGGAATAC CTAAAGAATG TCCTAACAAA                           40
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             86
ATGGCTCTGC TCGGACACTC TCTGGGCATC CCCTGGGCTC CCCTGAGCTC CTGCCCCAGC     60
CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC CAACTCCATA GCGGCCTTTT CCTCTACCAG    120
GGGCTCCTGC AGGCCCTGGA AGGGATATCC CCCGAGTTGG GTCCCACCTT GGACACACTG    180
CAGCTGGACG TCGCCGACTT TGCCACCACC ATCTGGCAGC AGATGGAAGA ACTGGGAATG    240
GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC    300
CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT GTCGTACCGC    360
GTTCTACGCC ACCTTGCGCA GCCCACACCA TTGGGCCCTG CCAGCTCCCT GCCCCAGAGC    420
TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA AAGATCCAGG GCGATGGCGC AGCGCTCCAG    480
GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             87
ATGGCTCAAC TCCATAGCGG CCTTTTCCTC TACCAGGGGC TCCTGCAGGC CCTGGAAGGG     60
ATATCCCCCG AGTTGGGTCC CACCTTGGAC ACACTGCAGC TGGACGTCGC CGACTTTGCC    120
ACCACCATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC CTGCCCTGCA GCCCACCCAG    180
GGTGCCATGC CGGCCTTCGC CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT    240
AGCCATCTGC AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC    300
ACACCATTGG GCCCTGCCAG CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA    360
GTGAGAAAGA TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC CACCTACAAG    420
CTGTGCCACC CCGAGGAGCT GGTGCTGCTC GGACACTCTC TGGGCATCCC CTGGGCTCCC    480
CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG CTGGCAGGCT GCTTGAGCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             88
ATGGCTCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG AAGGGATATC CCCCGAGTTG     60
GGTCCCACCT TGGACACACT GCAGCTGGAC GTCGCCGACT TTGCCACCAC CATCTGGCAG    120
CAGATGGAAG AACTGGGAAT GGCCCCTGCC CTGCAGCCCA CCCAGGGTGC CATGCCGGCC    180
TTCGCCTCTG CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC    240
TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCACACC ATTGGGCCCT    300
GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC AAGTCTTTAG AGCAAGTGAG AAAGATCCAG    360
GGCGATGGCG CAGCGCTCCA GGAGAAGCTG TGTGCCACCT ACAAGCTGTG CCACCCCGAG    420
GAGCTGGTGC TGCTCGGACA CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC    480
AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG AGCCAACTCC ATAGCGGCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             89
ATGGCTCTCT ACCAGGGGCT CCTGCAGGCC CTGGAAGGGA TATCCCCCGA GTTGGGTCCC     60
ACCTTGGACA CACTGCAGCT GGACGTCGCC GACTTTGCCA CCACCATCTG GCAGCAGATG    120
GAAGAACTGG GAATGGCCCC TGCCCTGCAG CCCACCCAGG GTGCCATGCC GGCCTTCGCC    180
TCTGCTTTCC AGCGCCGGGC AGGAGGGGTC CTGGTTGCTA GCCATCTGCA GAGCTTCCTG    240
GAGGTGTCGT ACCGCGTTCT ACGCCACCTT GCGCAGCCCA CACCATTGGG CCCTGCCAGC    300
TCCCTGCCCC AGAGCTTCCT GCTCAAGTCT TTAGAGCAAG TGAGAAAGAT CCAGGGCGAT    360
GGCGCAGCGC TCCAGGAGAA GCTGTGTGCC ACCTACAAGC TGTGCCACCC CGAGGAGCTG    420
GTGCTGCTCG GACACTCTCT GGGCATCCCC TGGGCTCCCC TGAGCTCCTG CCCCAGCCAG    480
GCCCTGCAGC TGGCAGGCTG CTTGAGCCAA CTCCATAGCG GCCTTTTCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             90
ATGGCTGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA CCTTGGACAC ACTGCAGCTG     60
GACGTCGCCG ACTTTGCCAC CACCATCTGG CAGCAGATGG AAGAACTGGG AATGGCCCCT    120
GCCCTGCAGC CCACCCAGGG TGCCATGCCG GCCTTCGCCT CTGCTTTCCA GCGCCGGGCA    180
GGAGGGGTCC TGGTTGCTAG CCATCTGCAG AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA    240
CGCCACCTTG CGCAGCCCAC ACCATTGGGC CCTGCCAGCT CCCTGCCCCA GAGCTTCCTG    300
CTCAAGTCTT TAGAGCAAGT GAGAAAGATC CAGGGCGATG GCGCAGCGCT CCAGGAGAAG    360
CTGTGTGCCA CCTACAAGCT GTGCCACCCC GAGGAGCTGG TGCTGCTCGG ACACTCTCTG    420
GGCATCCCCT GGGCTCCCCT GAGCTCCTGC CCCAGCCAGG CCCTGCAGCT GGCAGGCTGC    480
TTGAGCCAAC TCCATAGCGG CCTTTTCCTC TACCAGGGGC TCCTGCAGTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             91
ATGGCTGACT TTGCCACCAC CATCTGGCAG CAGATGGAAG AACTGGGAAT GGCCCCTGCC     60
CTGCAGCCCA CCCAGGGTGC CATGCCGGCC TTCGCCTCTG CTTTCCAGCG CCGGGCAGGA    120
GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC TTCCTGGAGG TGTCGTACCG CGTTCTACGC    180
CACCTTGCGC AGCCCACACC ATTGGGCCCT GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC    240
AAGTCTTTAG AGCAAGTGAG AAAGATCCAG GGCGATGGCG CAGCGCTCCA GGAGAAGCTG    300
TGTGCCACCT ACAAGCTGTG CCACCCCGAG GAGCTGGTGC TGCTCGGACA CTCTCTGGGC    360
ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG    420
AGCCAACTCC ATAGCGGCCT TTTCCTCTAC CAGGGGCTCC TGCAGGCCCT GGAAGGGATA    480
TCCCCCGAGT TGGGTCCCAC CTTGGACACA CTGCAGCTGG ACGTCGCCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             92
ATGGCTATCT GGCAGCAGAT GGAAGAACTG GGAATGGCCC CTGCCCTGCA GCCCACCCAG     60
GGTGCCATGC CGGCCTTCGC CTCTGCTTTC CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT    120
AGCCATCTGC AGAGCTTCCT GGAGGTGTCG TACCGCGTTC TACGCCACCT TGCGCAGCCC    180
ACACCATTGG GCCCTGCCAG CTCCCTGCCC CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA    240
GTGAGAAAGA TCCAGGGCGA TGGCGCAGCG CTCCAGGAGA AGCTGTGTGC CACCTACAAG    300
CTGTGCCACC CCGAGGAGCT GGTGCTGCTC GGACACTCTC TGGGCATCCC CTGGGCTCCC    360
CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG CTGGCAGGCT GCTTGAGCCA ACTCCATAGC    420
GGCCTTTTCC TCTACCAGGG GCTCCTGCAG GCCCTGGAAG GGATATCCCC CGAGTTGGGT    480
CCCACCTTGG ACACACTGCA GCTGGACGTC GCCGACTTTG CCACCACCTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             93
ATGGCTCAGC AGATGGAAGA ACTGGGAATG GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC     60
ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT    120
CTGCAGAGCT TCCTGGAGGT GTCGTACCGC GTTCTACGCC ACCTTGCGCA GCCCACACCA    180
TTGGGCCCTG CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA    240
AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA CAAGCTGTGC    300
CACCCCGAGG AGCTGGTGCT GCTCGGACAC TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC    360
TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA GGCTGCTTGA GCCAACTCCA TAGCGGCCTT    420
TTCCTCTACC AGGGGCTCCT GCAGGCCCTG GAAGGGATAT CCCCCGAGTT GGGTCCCACC    480
TTGGACACAC TGCAGCTGGA CGTCGCCGAC TTTGCCACCA CCATCTGGTA ATAA          534
 
           
           
             
               534 base pairs 
               nucleic acid 
               single 
               linear 
             
             
               other nucleic acid 
               /desc = “DNA (synthetic)” 
             
             94
ATGGCTTTGT TAGGACATTC TTTAGGTATT CCATGGGCTC CTCTGAGCTC CTGCCCCAGC     60
CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC CAACTCCATA GCGGCCTTTT CCTCTACCAG    120
GGGCTCCTGC AGGCCCTGGA AGGGATATCC CCCGAGTTGG GTCCCACCTT GGACACACTG    180
CAGCTGGACG TCGCCGACTT TGCCACCACC ATCTGGCAGC AGATGGAAGA ACTGGGAATG    240
GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC    300
CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT GTCGTACCGC    360
GTTCTACGCC ACCTTGCGCA GCCCACACCA TTGGGCCCTG CCAGCTCCCT GCCCCAGAGC    420
TTCCTGCTCA AGTCTTTAGA GCAAGTGAGA AAGATCCAGG GCGATGGCGC AGCGCTCCAG    480
GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGTA ATAA          534
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             95
Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys
1               5                   10                  15
Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser
            20                  25                  30
Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
        35                  40                  45
Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp
    50                  55                  60
Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro
65                  70                  75                  80
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe
                85                  90                  95
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe
            100                 105                 110
Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro
        115                 120                 125
Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu
    130                 135                 140
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
145                 150                 155                 160
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             96
Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu
1               5                   10                  15
Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu
            20                  25                  30
Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu
        35                  40                  45
Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe
    50                  55                  60
Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His
65                  70                  75                  80
Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala
                85                  90                  95
Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu
            100                 105                 110
Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala
        115                 120                 125
Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu
    130                 135                 140
Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser
145                 150                 155                 160
Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             97
Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro
1               5                   10                  15
Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe
            20                  25                  30
Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala
        35                  40                  45
Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln
    50                  55                  60
Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu
65                  70                  75                  80
Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu
                85                  90                  95
Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu
            100                 105                 110
Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu
        115                 120                 125
Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly
    130                 135                 140
His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln
145                 150                 155                 160
Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             98
Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu
1               5                   10                  15
Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr
            20                  25                  30
Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln
        35                  40                  45
Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg
    50                  55                  60
Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val
65                  70                  75                  80
Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro
                85                  90                  95
Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val
            100                 105                 110
Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala
        115                 120                 125
Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser
    130                 135                 140
Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu
145                 150                 155                 160
Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             99
Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu
1               5                   10                  15
Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu
            20                  25                  30
Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro
        35                  40                  45
Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala
    50                  55                  60
Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His
65                  70                  75                  80
Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser
                85                  90                  95
Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly
            100                 105                 110
Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro
        115                 120                 125
Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro
    130                 135                 140
Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser
145                 150                 155                 160
Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             100
Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala
1               5                   10                  15
Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala
            20                  25                  30
Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser
        35                  40                  45
Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr
    50                  55                  60
Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser
65                  70                  75                  80
Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu
                85                  90                  95
Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu
            100                 105                 110
Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro
        115                 120                 125
Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly
    130                 135                 140
Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro
145                 150                 155                 160
Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             101
Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro
1               5                   10                  15
Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala
            20                  25                  30
Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser
        35                  40                  45
Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala
    50                  55                  60
Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg
65                  70                  75                  80
Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr
                85                  90                  95
Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu
            100                 105                 110
Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln
        115                 120                 125
Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln
    130                 135                 140
Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr
145                 150                 155                 160
Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             102
Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln
1               5                   10                  15
Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly
            20                  25                  30
Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg
        35                  40                  45
Val Leu Arg His Leu Ala Gln Pro Thr Pro Leu Gly Pro Ala Ser Ser
    50                  55                  60
Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile
65                  70                  75                  80
Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys
                85                  90                  95
Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile
            100                 105                 110
Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala
        115                 120                 125
Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu
    130                 135                 140
Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp
145                 150                 155                 160
Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp
                165                 170
 
           
           
             
               174 amino acids 
               amino acid 
               single 
               linear 
             
             
               protein 
             
             103
Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys
1               5                   10                  15
Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser
            20                  25                  30
Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
        35                  40                  45
Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp
    50                  55                  60
Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro
65                  70                  75                  80
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe
                85                  90                  95
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe
            100                 105                 110
Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Thr Pro
        115                 120                 125
Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu
    130                 135                 140
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
145                 150                 155                 160
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val
                165                 170
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             104
CAGAGCTTCC TGCTCAAGTC TTTAGAGCAA GTGAGGAAGA TCCAGGGCGA TGGCGCAGCG     60
CTCCAGGAGA AGCTGTGTGC CACCTACAAG CTGTGCCACC CCGAGGAGCT GGTGCTGCTC    120
GGACACTCTC TGGGCATCCC CTGGGCTCCC CTGAGCTCCT GCCCCAGCCA GGCCCTGCAG    180
CTGGCAGGCT GCTTGAGCCA ACTCCATAGC GGCCTTTTCC TCTACCAGGG GCTCCTGCAG    240
GCCCTGGAAG GGATATCCCC CGAGTTGGGT CCCACCTTGG ACACACTGCA GCTGGACGTC    300
GCCGACTTTG CCACCACCAT CTGGCAGCAG ATGGAAGAAC TGGGAATGGC CCCTGCCCTG    360
CAGCCCACCC AGGGTGCCAT GCCGGCCTTC GCCTCTGCTT TCCAGCGCCG GGCAGGAGGG    420
GTCCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT CGTACCGCGT TCTACGCCAC    480
CTTGCGCAGC CCGACATGGC TACACCATTA GGCCCTGCCA GCTCCCTGCC C             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             105
GAACTGGGAA TGGCCCCTGC CCTGCAGCCC ACCCAGGGTG CCATGCCGGC CTTCGCCTCT     60
GCTTTCCAGC GCCGGGCAGG AGGGGTCCTG GTTGCTAGCC ATCTGCAGAG CTTCCTGGAG    120
GTGTCGTACC GCGTTCTACG CCACCTTGCG CAGCCCGACA TGGCTACACC ATTAGGCCCT    180
GCCAGCTCCC TGCCCCAGAG CTTCCTGCTC AAGTCTTTAG AGCAAGTGAG GAAGATCCAG    240
GGCGATGGCG CAGCGCTCCA GGAGAAGCTG TGTGCCACCT ACAAGCTGTG CCACCCCGAG    300
GAGCTGGTGC TGCTCGGACA CTCTCTGGGC ATCCCCTGGG CTCCCCTGAG CTCCTGCCCC    360
AGCCAGGCCC TGCAGCTGGC AGGCTGCTTG AGCCAACTCC ATAGCGGCCT TTTCCTCTAC    420
CAGGGGCTCC TGCAGGCCCT GGAAGGGATA TCCCCCGAGT TGGGTCCCAC CTTGGACACA    480
CTGCAGCTGG ACGTCGCCGA CTTTGCCACC ACCATCTGGC AGCAGATGGA A             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             106
GGAATGGCCC CTGCCCTGCA GCCCACCCAG GGTGCCATGC CGGCCTTCGC CTCTGCTTTC     60
CAGCGCCGGG CAGGAGGGGT CCTGGTTGCT AGCCATCTGC AGAGCTTCCT GGAGGTGTCG    120
TACCGCGTTC TACGCCACCT TGCGCAGCCC GACATGGCTA CACCATTAGG CCCTGCCAGC    180
TCCCTGCCCC AGAGCTTCCT GCTCAAGTCT TTAGAGCAAG TGAGGAAGAT CCAGGGCGAT    240
GGCGCAGCGC TCCAGGAGAA GCTGTGTGCC ACCTACAAGC TGTGCCACCC CGAGGAGCTG    300
GTGCTGCTCG GACACTCTCT GGGCATCCCC TGGGCTCCCC TGAGCTCCTG CCCCAGCCAG    360
GCCCTGCAGC TGGCAGGCTG CTTGAGCCAA CTCCATAGCG GCCTTTTCCT CTACCAGGGG    420
CTCCTGCAGG CCCTGGAAGG GATATCCCCC GAGTTGGGTC CCACCTTGGA CACACTGCAG    480
CTGGACGTCG CCGACTTTGC CACCACCATC TGGCAGCAGA TGGAAGAACT G             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             107
TTCCTGCTCA AGTCTTTAGA GCAAGTGAGG AAGATCCAGG GCGATGGCGC AGCGCTCCAG     60
GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT GCTCGGACAC    120
TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA    180
GGCTGCTTGA GCCAACTCCA TAGCGGCCTT TTCCTCTACC AGGGGCTCCT GCAGGCCCTG    240
GAAGGGATAT CCCCCGAGTT GGGTCCCACC TTGGACACAC TGCAGCTGGA CGTCGCCGAC    300
TTTGCCACCA CCATCTGGCA GCAGATGGAA GAACTGGGAA TGGCCCCTGC CCTGCAGCCC    360
ACCCAGGGTG CCATGCCGGC CTTCGCCTCT GCTTTCCAGC GCCGGGCAGG AGGGGTCCTG    420
GTTGCTAGCC ATCTGCAGAG CTTCCTGGAG GTGTCGTACC GCGTTCTACG CCACCTTGCG    480
CAGCCCGACA TGGCTACACC ATTAGGCCCT GCCAGCTCCC TGCCCCAGAG C             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             108
AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA CGCCACCTTG CGCAGCCCGA CATGGCTACA     60
CCATTAGGCC CTGCCAGCTC CCTGCCCCAG AGCTTCCTGC TCAAGTCTTT AGAGCAAGTG    120
AGGAAGATCC AGGGCGATGG CGCAGCGCTC CAGGAGAAGC TGTGTGCCAC CTACAAGCTG    180
TGCCACCCCG AGGAGCTGGT GCTGCTCGGA CACTCTCTGG GCATCCCCTG GGCTCCCCTG    240
AGCTCCTGCC CCAGCCAGGC CCTGCAGCTG GCAGGCTGCT TGAGCCAACT CCATAGCGGC    300
CTTTTCCTCT ACCAGGGGCT CCTGCAGGCC CTGGAAGGGA TATCCCCCGA GTTGGGTCCC    360
ACCTTGGACA CACTGCAGCT GGACGTCGCC GACTTTGCCA CCACCATCTG GCAGCAGATG    420
GAAGAACTGG GAATGGCCCC TGCCCTGCAG CCCACCCAGG GTGCCATGCC GGCCTTCGCC    480
TCTGCTTTCC AGCGCCGGGC AGGAGGGGTC CTGGTTGCTA GCCATCTGCA G             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             109
AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA CGCCACCTTG CGCAGCCCGA CATGGCTACA     60
CCATTAGGCC CTGCCAGCTC CCTGCCCCAG AGCTTCCTGC TCAAGTCTTT AGAGCAAGTG    120
AGGAAGATCC AGGGCGATGG CGCAGCGCTC CAGGAGAAGC TGTGTGCCAC CTACAAGCTG    180
TGCCACCCCG AGGAGCTGGT GCTGCTCGGA CACTCTCTGG GCATCCCCTG GGCTCCCCTG    240
AGCTCCTGCC CCAGCCAGGC CCTGCAGCTG GCAGGCTGCT TGAGCCAACT CCATAGCGGC    300
CTTTTCCTCT ACCAGGGGCT CCTGCAGGCC CTGGAAGGGA TATCCCCCGA GTTGGGTCCC    360
ACCTTGGACA CACTGCAGCT GGACGTCGCC GACTTTGCCA CCACCATCTG GCAGCAGATG    420
GAAGAACTGG GAATGGCCCC TGCCCTGCAG CCCACCCAGG GTGCCATGCC GGCCTTCGCC    480
TCTGCTTTCC AGCGCCGGGC AGGAGGGGTC CTGGTTGCTA GCCATCTGCA G             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             110
TTAGGCCCTG CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGG     60
AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA CAAGCTGTGC    120
CACCCCGAGG AGCTGGTGCT GCTCGGACAC TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC    180
TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA GGCTGCTTGA GCCAACTCCA TAGCGGCCTT    240
TTCCTCTACC AGGGGCTCCT GCAGGCCCTG GAAGGGATAT CCCCCGAGTT GGGTCCCACC    300
TTGGACACAC TGCAGCTGGA CGTCGCCGAC TTTGCCACCA CCATCTGGCA GCAGATGGAA    360
GAACTGGGAA TGGCCCCTGC CCTGCAGCCC ACCCAGGGTG CCATGCCGGC CTTCGCCTCT    420
GCTTTCCAGC GCCGGGCAGG AGGGGTCCTG GTTGCTAGCC ATCTGCAGAG CTTCCTGGAG    480
GTGTCGTACC GCGTTCTACG CCACCTTGCG CAGCCCGACA TGGCTACACC A             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             111
CTGCTCGGAC ACTCTCTGGG CATCCCCTGG GCTCCCCTGA GCTCCTGCCC CAGCCAGGCC     60
CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA CCAGGGGCTC    120
CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA CCTTGGACAC ACTGCAGCTG    180
GACGTCGCCG ACTTTGCCAC CACCATCTGG CAGCAGATGG AAGAACTGGG AATGGCCCCT    240
GCCCTGCAGC CCACCCAGGG TGCCATGCCG GCCTTCGCCT CTGCTTTCCA GCGCCGGGCA    300
GGAGGGGTCC TGGTTGCTAG CCATCTGCAG AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA    360
CGCCACCTTG CGCAGCCCGA CATGGCTACA CCATTAGGCC CTGCCAGCTC CCTGCCCCAG    420
AGCTTCCTGC TCAAGTCTTT AGAGCAAGTG AGGAAGATCC AGGGCGATGG CGCAGCGCTC    480
CAGGAGAAGC TGTGTGCCAC CTACAAGCTG TGCCACCCCG AGGAGCTGGT G             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             112
CCCCTGAGCT CCTGCCCCAG CCAGGCCCTG CAGCTGGCAG GCTGCTTGAG CCAACTCCAT     60
AGCGGCCTTT TCCTCTACCA GGGGCTCCTG CAGGCCCTGG AAGGGATATC CCCCGAGTTG    120
GGTCCCACCT TGGACACACT GCAGCTGGAC GTCGCCGACT TTGCCACCAC CATCTGGCAG    180
CAGATGGAAG AACTGGGAAT GGCCCCTGCC CTGCAGCCCA CCCAGGGTGC CATGCCGGCC    240
TTCGCCTCTG CTTTCCAGCG CCGGGCAGGA GGGGTCCTGG TTGCTAGCCA TCTGCAGAGC    300
TTCCTGGAGG TGTCGTACCG CGTTCTACGC CACCTTGCGC AGCCCGACAT GGCTACACCA    360
TTAGGCCCTG CCAGCTCCCT GCCCCAGAGC TTCCTGCTCA AGTCTTTAGA GCAAGTGAGG    420
AAGATCCAGG GCGATGGCGC AGCGCTCCAG GAGAAGCTGT GTGCCACCTA CAAGCTGTGC    480
CACCCCGAGG AGCTGGTGCT GCTCGGACAC TCTCTGGGCA TCCCCTGGGC T             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             113
CAGGCCCTGC AGCTGGCAGG CTGCTTGAGC CAACTCCATA GCGGCCTTTT CCTCTACCAG     60
GGGCTCCTGC AGGCCCTGGA AGGGATATCC CCCGAGTTGG GTCCCACCTT GGACACACTG    120
CAGCTGGACG TCGCCGACTT TGCCACCACC ATCTGGCAGC AGATGGAAGA ACTGGGAATG    180
GCCCCTGCCC TGCAGCCCAC CCAGGGTGCC ATGCCGGCCT TCGCCTCTGC TTTCCAGCGC    240
CGGGCAGGAG GGGTCCTGGT TGCTAGCCAT CTGCAGAGCT TCCTGGAGGT GTCGTACCGC    300
GTTCTACGCC ACCTTGCGCA GCCCGACATG GCTACACCAT TAGGCCCTGC CAGCTCCCTG    360
CCCCAGAGCT TCCTGCTCAA GTCTTTAGAG CAAGTGAGGA AGATCCAGGG CGATGGCGCA    420
GCGCTCCAGG AGAAGCTGTG TGCCACCTAC AAGCTGTGCC ACCCCGAGGA GCTGGTGCTG    480
CTCGGACACT CTCTGGGCAT CCCCTGGGCT CCCCTGAGCT CCTGCCCCAG C             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             114
CTGCAGCTGG CAGGCTGCTT GAGCCAACTC CATAGCGGCC TTTTCCTCTA CCAGGGGCTC     60
CTGCAGGCCC TGGAAGGGAT ATCCCCCGAG TTGGGTCCCA CCTTGGACAC ACTGCAGCTG    120
GACGTCGCCG ACTTTGCCAC CACCATCTGG CAGCAGATGG AAGAACTGGG AATGGCCCCT    180
GCCCTGCAGC CCACCCAGGG TGCCATGCCG GCCTTCGCCT CTGCTTTCCA GCGCCGGGCA    240
GGAGGGGTCC TGGTTGCTAG CCATCTGCAG AGCTTCCTGG AGGTGTCGTA CCGCGTTCTA    300
CGCCACCTTG CGCAGCCCGA CATGGCTACA CCATTAGGCC CTGCCAGCTC CCTGCCCCAG    360
AGCTTCCTGC TCAAGTCTTT AGAGCAAGTG AGGAAGATCC AGGGCGATGG CGCAGCGCTC    420
CAGGAGAAGC TGTGTGCCAC CTACAAGCTG TGCCACCCCG AGGAGCTGGT GCTGCTCGGA    480
CACTCTCTGG GCATCCCCTG GGCTCCCCTG AGCTCCTGCC CCAGCCAGGC C             531
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             115
CTGGCAGGCT GCTTGAGCCA ACTCCATAGC GGCCTTTTCC TCTACCAGGG GCTCCTGCAG     60
GCCCTGGAAG GGATATCCCC CGAGTTGGGT CCCACCTTGG ACACACTGCA GCTGGACGTC    120
GCCGACTTTG CCACCACCAT CTGGCAGCAG ATGGAAGAAC TGGGAATGGC CCCTGCCCTG    180
CAGCCCACCC AGGGTGCCAT GCCGGCCTTC GCCTCTGCTT TCCAGCGCCG GGCAGGAGGG    240
GTCCTGGTTG CTAGCCATCT GCAGAGCTTC CTGGAGGTGT CGTACCGCGT TCTACGCCAC    300
CTTGCGCAGC CCGACATGGC TACACCATTA GGCCCTGCCA GCTCCCTGCC CCAGAGCTTC    360
CTGCTCAAGT CTTTAGAGCA AGTGAGGAAG ATCCAGGGCG ATGGCGCAGC GCTCCAGGAG    420
AAGCTGTGTG CCACCTACAA GCTGTGCCAC CCCGAGGAGC TGGTGCTGCT CGGACACTCT    480
CTGGGCATCC CCTGGGCTCC CCTGAGCTCC TGCCCCAGCC AGGCCCTGCA G             531
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             116
Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly
1               5                   10                  15
Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys
            20                  25                  30
His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp
        35                  40                  45
Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys
    50                  55                  60
Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln
65                  70                  75                  80
Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu
                85                  90                  95
Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu
            100                 105                 110
Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro
        115                 120                 125
Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala
    130                 135                 140
Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His
145                 150                 155                 160
Leu Ala Gln Pro Asp Met Ala Thr Pro Leu Gly Pro Ala Ser Ser Leu
                165                 170                 175
Pro
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             117
Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro
1               5                   10                  15
Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala
            20                  25                  30
Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His
        35                  40                  45
Leu Ala Gln Pro Asp Met Ala Thr Pro Leu Gly Pro Ala Ser Ser Leu
    50                  55                  60
Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln
65                  70                  75                  80
Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu
                85                  90                  95
Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro
            100                 105                 110
Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly
        115                 120                 125
Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu
    130                 135                 140
Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr
145                 150                 155                 160
Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met
                165                 170                 175
Glu
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             118
Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe
1               5                   10                  15
Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His
            20                  25                  30
Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala
        35                  40                  45
Gln Pro Asp Met Ala Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln
    50                  55                  60
Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp
65                  70                  75                  80
Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His
                85                  90                  95
Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala
            100                 105                 110
Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu
        115                 120                 125
Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala
    130                 135                 140
Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln
145                 150                 155                 160
Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu
                165                 170                 175
Leu
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             119
Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly
1               5                   10                  15
Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro
            20                  25                  30
Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro
        35                  40                  45
Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser
    50                  55                  60
Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu
65                  70                  75                  80
Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu
                85                  90                  95
Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu
            100                 105                 110
Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe
        115                 120                 125
Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His
    130                 135                 140
Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala
145                 150                 155                 160
Gln Pro Asp Met Ala Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln
                165                 170                 175
Ser
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             120
Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro
1               5                   10                  15
Asp Met Ala Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe
            20                  25                  30
Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala
        35                  40                  45
Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu
    50                  55                  60
Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu
65                  70                  75                  80
Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln
                85                  90                  95
Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu
            100                 105                 110
Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp
        115                 120                 125
Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly
    130                 135                 140
Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala
145                 150                 155                 160
Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu
                165                 170                 175
Gln
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             121
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
1               5                   10                  15
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu
            20                  25                  30
Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser
        35                  40                  45
Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu
    50                  55                  60
Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu
65                  70                  75                  80
Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala
                85                  90                  95
Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu
            100                 105                 110
Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg
        115                 120                 125
Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu
    130                 135                 140
Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Asp Met Ala Thr
145                 150                 155                 160
Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser
                165                 170                 175
Leu
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             122
Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu
1               5                   10                  15
Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys
            20                  25                  30
Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu
        35                  40                  45
Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser
    50                  55                  60
Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu
65                  70                  75                  80
Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu
                85                  90                  95
Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala
            100                 105                 110
Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu
        115                 120                 125
Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg
    130                 135                 140
Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu
145                 150                 155                 160
Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Asp Met Ala Thr
                165                 170                 175
Pro
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             123
Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys
1               5                   10                  15
Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser
            20                  25                  30
Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser
        35                  40                  45
Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp
    50                  55                  60
Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro
65                  70                  75                  80
Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe
                85                  90                  95
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe
            100                 105                 110
Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Asp Met
        115                 120                 125
Ala Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu
    130                 135                 140
Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu
145                 150                 155                 160
Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu
                165                 170                 175
Val
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             124
Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu
1               5                   10                  15
Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala
            20                  25                  30
Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln
        35                  40                  45
Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu
    50                  55                  60
Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala
65                  70                  75                  80
Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser
                85                  90                  95
His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu
            100                 105                 110
Ala Gln Pro Asp Met Ala Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro
        115                 120                 125
Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Gly
    130                 135                 140
Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys
145                 150                 155                 160
His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp
                165                 170                 175
Ala
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             125
Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu
1               5                   10                  15
Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu
            20                  25                  30
Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala
        35                  40                  45
Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu
    50                  55                  60
Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg
65                  70                  75                  80
Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu
                85                  90                  95
Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Asp Met Ala Thr
            100                 105                 110
Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser
        115                 120                 125
Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu
    130                 135                 140
Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu
145                 150                 155                 160
Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro
                165                 170                 175
Ser
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             126
Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu
1               5                   10                  15
Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly
            20                  25                  30
Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr
        35                  40                  45
Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro
    50                  55                  60
Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala
65                  70                  75                  80
Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser
                85                  90                  95
Tyr Arg Val Leu Arg His Leu Ala Gln Pro Asp Met Ala Thr Pro Leu
            100                 105                 110
Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu
        115                 120                 125
Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu
    130                 135                 140
Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly
145                 150                 155                 160
His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln
                165                 170                 175
Ala
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             127
Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln
1               5                   10                  15
Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr
            20                  25                  30
Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp
        35                  40                  45
Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln
    50                  55                  60
Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly
65                  70                  75                  80
Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg
                85                  90                  95
Val Leu Arg His Leu Ala Gln Pro Asp Met Ala Thr Pro Leu Gly Pro
            100                 105                 110
Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val
        115                 120                 125
Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala
    130                 135                 140
Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser
145                 150                 155                 160
Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu
                165                 170                 175
Gln
 
           
           
             
               177 amino acids 
               amino acid 
               unknown 
               unknown 
             
             
               peptide 
             
             128
His Leu Ala Gln Pro Asp Met Ala Thr Pro Leu Gly Pro Ala Ser Ser
1               5                   10                  15
Leu Pro Gln Ser Phe Leu Leu Lys Ser Leu Glu Gln Val Arg Lys Ile
            20                  25                  30
Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys
        35                  40                  45
Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile
    50                  55                  60
Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala
65                  70                  75                  80
Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu
                85                  90                  95
Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp
            100                 105                 110
Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln
        115                 120                 125
Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala
    130                 135                 140
Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu
145                 150                 155                 160
Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu
                165                 170                 175
Arg
 
           
           
             
               531 base pairs 
               nucleic acid 
               unknown 
               unknown 
             
             
               other nucleic acid 
               /desc = “synthetic” 
             
             129
CACCTTGCGC AGCCCGACAT GGCTACACCA TTAGGCCCTG CCAGCTCCCT GCCCCAGAGC     60
TTCCTGCTCA AGTCTTTAGA GCAAGTGAGG AAGATCCAGG GCGATGGCGC AGCGCTCCAG    120
GAGAAGCTGT GTGCCACCTA CAAGCTGTGC CACCCCGAGG AGCTGGTGCT GCTCGGACAC    180
TCTCTGGGCA TCCCCTGGGC TCCCCTGAGC TCCTGCCCCA GCCAGGCCCT GCAGCTGGCA    240
GGCTGCTTGA GCCAACTCCA TAGCGGCCTT TTCCTCTACC AGGGGCTCCT GCAGGCCCTG    300
GAAGGGATAT CCCCCGAGTT GGGTCCCACC TTGGACACAC TGCAGCTGGA CGTCGCCGAC    360
TTTGCCACCA CCATCTGGCA GCAGATGGAA GAACTGGGAA TGGCCCCTGC CCTGCAGCCC    420
ACCCAGGGTG CCATGCCGGC CTTCGCCTCT GCTTTCCAGC GCCGGGCAGG AGGGGTCCTG    480
GTTGCTAGCC ATCTGCAGAG CTTCCTGGAG GTGTCGTACC GCGTTCTACG C             531