Abstract:
A fish killing and fish tissue sanitizing apparatus includes a tank, a water feed pipe extending to the tank, and an electromechanical transducer in pressure-wave transmitting relationship to the tank for generating ultrasonic pressure waves in water contained in the tank. An electrical signal generator is operatively connected to the transducer for energizing same with an alternating electrical signal. A sensor is in operative contact with water contained in the tank for detecting transient and inertial cavitation occurring within the water in the tank.

Description:
FIELD OF THE INVENTION  
       [0001]     This invention relates generally to a method and an associated device or apparatus for treating living tissue with ultrasonic wave energy. More specifically, this invention relates to a method and to an associated device or apparatus for catastrophic low-frequency, medium intensity ultrasonic irradiation of living fish tissue disposed in submicron filtered/degassed water. More particularly, this invention relates to a method and an associated apparatus for achieving instantaneous unconsciousness and insensibility of fish while concomitantly sanitizing its tissue until death supervenes  
       BACKGROUND OF THE INVENTION  
       [0002]     With specific reference to fish-farms, existing regulatory authorities recommend no specific method for slaughtering fish and as a result, some or all of the following techniques may be employed: 
    1) Asphyxiation—suffocating the fish by removal from water. Farmed trout are commonly “harvested” by removal from water into bins in which they suffocate. Fish farmers have started to put live fish into bins containing ice according to Bristol University&#39;s Department of Meat Animal Science. The researchers also found when fish were removed from water they can often still feel what is happening to them for almost 15 minutes at low temperatures. The researchers concluded that the practice of suffocating fish on ice could unnecessarily prolong the time to unconsciousness. (Kestin, Wotten &amp; Gregory, 1991.)     2) Bleeding—cutting the fish gills causing death by blood loss. This method may be preceded by stunning the fish in a tank containing carbon-dioxide saturated water. Welfare concerns arise with this stunning method as the “fish try to escape violently” when put into the tank, (Kestin, 4.2.92). The fish are usually unable to move within one minute and do not lose sensibility for 4-5-minutes. Fish could therefore have their gills cut whilst still conscious if lack of movement was mistaken for unconsciousness. If gill-slitting was carried out unsatisfactorily, it is possible that fish could recover consciousness whilst bleeding. For salmon, bleeding is recommended if the fish are intended to be smoked. This ensures the blood vessels are not readily apparent in the finished product. (Shepherd &amp; Bromage, 1988.)    
 
         [0005]     Norwegian fish farmers slaughter salmon by cutting the main blood vessels located in the head. The fish are then returned to the water where they subsequently weaken and die from blood loss. (Sedgewick, 1988.) 
    3) Concussion—killing by a blow to the head with a small, hand-held club. This slaughter method can cause instantaneous unconsciousness in the fish if done properly. However, the potential for improper stunning and injury to the fish is considerable. (Kestin, 4.2.92.)     4) Electrocution—killing by placing fish in a large tank through which electricity is allowed to flow for a few seconds. The electrical current and its frequency has to be at just the right level to stun the fish without burning the tissue. In early trials the system used too much electricity and stunned too few fish to be commercially practical. (Anthony Browne, The Times, 5.3.2003)    
 
         [0008]     The Bristol University research team concluded that currently practiced slaughter methods for farmed fish fall far short of the requirement for instantaneous unconsciousness. Concussion and electrocution methods have been suggested as having the most potential for achieving instantaneous unconsciousness in fish, (Kestin, Wotten &amp; Gregory, 1991).  
         [0009]     Currently, following their slaughter, fish are cleaned externally then prepared for market. In its slaughtered state, fish tissue will contain whatever toxic pollutants, parasites, bacterial and viral pathogens it is contaminated with.  
         [0010]     It is widely recognized that intensive and stressful conditions, associated with fish farming, can predispose fish to attack from disease and parasitic infection and where diseases such as bacterial septicaemia and gill infections, and bacterial gill disease prevail.  
         [0011]     Bacterial diseases are currently treated by the use of antibiotics mixed in with fish feed. Potential human health hazards can arise from the high incidence of farmed-fish disease and its subsequent treatment. Prolonged use of antibiotics in fish can lead to the development of drug-resistant strains of bacteria. It is feared that such drug resistance could then be transferred from fish bacteria to human bacteria in the digestive tract with potentially disastrous results. Many antibiotics that treat fish diseases, such as tetracycline and chloramphenicol, are also used in human medicine. (Shepherd &amp; Bromage, 1998.)  
         [0012]     Drug resistance may be unknowingly picked up by a human via the above route. If that person were to fall ill and be treated by a doctor using similar antibiotic, the drug may have been rendered less efficient or ineffective.  
         [0013]     Another example, with regard to toxic PCB infestation, farmed salmon are fed from a global supply of fish-meal and fish-oil from small open sea fish which studies show are the source of PCB&#39;s (Polychlorinated Biphenyls) in most farmed salmon. In three independent studies scientists tested 37 fish-meal samples from six countries and found PCB contamination in nearly every sample. (Jacobs 2002, Easton 2002, and CIFA 1999.)  
         [0014]     Humans can ingest PCB&#39;s from eating contaminated fish and there is broad multiple governmental agreement from multiple governmental agencies that consumption of PCB&#39;s are expected to cause cancer and alter brain development in humans.  
       OBJECTS OF THE INVENTION  
       [0015]     A general object of the present invention is to provide an improved method for killing fish.  
         [0016]     A related general object of the present invention is to provide an improved method for killing fish and sanitizing the fish tissue.  
         [0017]     More specifically, it is an object of the present invention to provide a method for inducing instantaneous fish unconsciousness and concomitantly sanitizing fish tissue.  
         [0018]     An even more specific object of the present invention is to provide a method wherein ultrasonic irradiation is generated that has sufficient acoustic pressure to effect instantaneous unconsciousness in fish thereby maintaining insensibility of the fish to pain until death supervenes.  
         [0019]     A parallel object of the present invention is to provide such a method that also effect a rapid safe transformation of toxic pollutants, such as DDTs, polychlorinated biphenyls (PCB&#39;s) and killing of pathogenic bacteria, viruses and parasites that reside in slaughtered fish tissue.  
         [0020]     These and other objects of the present invention will be apparent from the drawings and descriptions herein. Although every object of the invention is attained in at least one embodiment of the invention, there is not necessarily any single embodiment of the invention in which all objects are attained.  
       SUMMARY OF THE INVENTION  
       [0021]     An ultrasonic sound pressure level of 32 Pa is not harmful to fish while a pressure level of 1,000 Pa is harmful to many fish, (Hastings, 1990) and an ultrasonic pressure of 266,000 Pa is fatal to most fish. (Norris &amp; Mohl, 1983.)  
         [0022]     Pressure sensitivity varies with fish-species and to avoid “overkill” during the “slaughtering phase”, the lowest ultrasonic pressure necessary to effect a particular species&#39; instantaneous unconsciousness and continuing insensibility until death supervenes must be determined experimentally by applying a subaqeuous low frequency, adjustable peak amplitude ultrasonic pressure wave having equal compressional and rarefactional cycles in the approximate pressure range 75 Pa to 300 kPa.  
         [0023]     For each particular fish species to experience immediate, predictable massive, irreparable internal organ and vascular damage, this invention utilizes submicron filtered degassed tap water whose properties permit propagation of a sinusoidal ultrasonic pressure wave without significant amplitude attenuation throughout the water mass contained by the fish-holding tank. While the slaughtering peak pressure amplitude selected for each particular fish species is being applied, the following concomitant fish-tissue sanitization process ensues.  
         [0024]     All fish exhibit a high-water tissue content. For example, Atlantic Salmon comprises 32% dry matter and 68% water. Tank water of different salinity, temperature and pressure holds differing amounts of oxygen, nitrogen and other gases called air. Given time, the gas pressure in the tank will equalize and become the same pressure as the air over it. Subsequently the gas pressure in fish tissue and bloodstream will become the same as in the water. The air pressure is the sum of the partial pressures of the individual gases, (primarily nitrogen, 78% and oxygen, 21%) that constitute air.  
         [0025]     Oxygen moderately above saturation in water is not typically a problem because fish use oxygen to breathe. However, since nitrogen is the most common of the inert gases in fresh or salt water systems and is not metabolized by fish it is the gas most commonly associated with bubble formation in fish. Nitrogen is an inert gas normally stored throughout fish tissues and fluids in a physical solution. When a fish is exposed to decreased hydrostatic and/or barometric pressures, the nitrogen gas dissolved in the fish tissues and fluids becomes supersaturated and comes out of solution. If the nitrogen is forced to leave the solution too rapidly, bubbles form in different parts of the fish, causing a variety of signs and symptoms.  
         [0026]     Fish sense high gas pressures. Like a diver, fish will go deeper in the tank to compress the gases and thereby prevent nitrogen bubble formation in their blood and tissue. Nitrogen enters a fish through its gills, just like oxygen. It is then carried to the tissue by the blood. Once distributed, nitrogen remains in the tissue while oxygen is consumed.  
         [0027]     When low frequency medium intensity ultrasonic pressure waves are propagated through fish undergoing slaughter, the negative pressure wave will cause the nitrogen in the fish tissue and blood to leave solution very rapidly, forming bubbles which under the influence of the alternating negative and positive pressure portions of the low frequency medium intensity ultrasound will culminate in transient cavitation bubble imploding events.  
         [0028]     The associated chemical effects of ultrasound transient cavitation implosions are explained in terms of reactions occurring inside, at the interface, or at some distance away from the cavitating bubbles. In the interior of an imploding cavitation bubble, extreme but transient conditions are known to exist. Temperatures approaching 5,000K have been estimated, and pressures of several hundred atmospheres have been calculated.  
         [0029]     Temperatures of the order of 2,000K have been estimated for the interfacial region surrounding an imploding bubble based on observed reactivity. During bubble implosion, which occurs within 100 nsec, H 2 O undergoes thermal dissociation to yield hydroxyl radicals and hydrogen atoms. Sonochemical reactions are characterized by the simultaneous occurrence of supercritical water reactions, direct pyrolyses, and radical reactions, especially with solute concentrations.  
         [0030]     The sonochemical degradation of a variety of chemical contaminants in aqueous solution has been previously reported. (Kotrounarou et al., 1991, 1992a,b.) Substrates such as chlorinated hydrocarbons, (PCB s &amp; DDT s), pesticides, phenols and esters are transformed into short-chain organic acids, CO2 and inorganic ions as the final products. Ultrasonic transient cavitation appears to be an effective method for destruction of organic contaminants in water because of localized high concentrations of oxidizing species such as hydroxyl radicals and hydrogen peroxide in solution, high localized temperatures and pressures and the formation of transient supercritical water. (Hua et al. 1995.)  
         [0031]     With a non-submicron filtered, non-degassed water mass surrounding a fish exterior, the water&#39;s occluded micron-sized and larger particles may contain sufficient trapped gas to evolve into transient cavitation prone bubbles when irradiated with low frequency medium intensity ultrasound.  
         [0032]     To ensure consistent and repeatable fish slaughtering/sanitization settings, the water medium through which the low frequency ultrasonic pressure wave is propagated must remain sufficiently filtrated and degassed during subsequent fish slaughtering/sanitization processes. Precautionary sensing for the presence of transient cavitation bubbles in the water surrounding the fish is detected by the inventions microphone PZT transducers, (previously referred to in patent application Ser. No. 10/676,061), which provide a microcomputer with the signal necessary for it to shut down ultrasonic transmission until the necessary degassification and particulate size reduction exchange in the tank has been effected.  
         [0033]     These precautions are necessary because transient bubble cavitation occurring in close proximity to the fish exterior will bombard its flesh with imploding high velocity bubble jets possibly causing an unsightly outward appearance of the affected fish making it an undesirable product for market.  
         [0034]     Also, millions of transitioning vibrating bubbles in the water surrounding the fish provide a protective bubble-screen around the fish exterior which serves to attenuate the amplitude of the external pressure wave entering the fish by approximately 33 dB (greater than 1 micropascal). Such external ultrasonic pressure wave amplitude attenuation will stop transient bubble cavitation formation within the fish thereby preventing its sanitization, and will sustain consciousness and continuing sensibility to pain and suffering resulting from its exterior flesh being subjected to the forces and temperatures associated with transient bubble cavitation implosion events.  
         [0035]     An economic water supply origin for the above slaughtering/sanitization process is from a municipal supply source which is subsequently passed through an activated charcoal filter to remove its chlorine content and then through a submicron reverse osmosis filter to remove all larger particulate matter.  
         [0036]     The submicron filtered water output from the reverse osmosis device is pumped into an injector nozzle whose discharge is fed via a custom-designed combined right-angled elbow and on/off discharge faucet. The low pressure zone on the exit side of the internally Venturi-shaped nozzle serves to remove gas from the reverse osmosis processed water which is discharged to atmosphere as it leaves the faucet and before the water enters the fish-holding tank.  
         [0037]     After several slaughtering/sanitization processes and fish removals have been completed, either the detection of transient cavitation or presence of shed fish scales will require the fish-holding tank containing reverse osmosis filtered and degassed water to be drained and then refilled with untreated municipal tap water and irradiated with low frequency, medium intensity subaqueous applied ultrasound for the time-period necessary to fully sanitize the tank.  
         [0038]     Accordingly, a fish killing and fish tissue sanitizing apparatus comprises, pursuant to the present invention, a tank, a water feed pipe extending to the tank, an electromechanical transducer in pressure-wave transmitting relationship to the tank for generating ultrasonic pressure waves in water contained in the tank, an electrical signal generator operatively connected to the transducer for energizing same with an alternating electrical signal, and a sensor in operative contact with water contained in the tank for detecting transient and inertial cavitation occurring within the water in the tank.  
         [0039]     Pursuant to further features of the present invention, the apparatus further comprises an injector disposed along the feed pipe proximate to a barrier thereof, the injector preferably taking the form of a Venturi injector, the feed pipe being coupled to a disinfectant reservoir and a valve being provided for introducing a disinfectant into a water stream flowing along the feed pipe, and the barrier being a wall of the pipe, the pipe having at least one elbow-type bend.  
         [0040]     Pursuant to another feature of the present invention, the sensor is a PZT probe.  
         [0041]     According to another feature of the present invention, the apparatus further comprising means operatively coupled to the signal generator for sweeping a frequency of an electrical excitation signal produced by the signal generator.  
         [0042]     A microprocessor may be operatively connected to the sensor, a display being operatively connected to the microprocessor for communicating to an operator a status of cavitation in the tank.  
         [0043]     The apparatus defined in claim  1  wherein the tank is one of two tanks communicating with one another via a barrier.  
         [0044]     An ultrasonic treatment method comprises, in accordance with the present invention, feeding water to a tank, disposing a living organism in the water, and thereafter generating ultrasonic pressure wave vibrations in the water of a frequency range and an intensity and duration to kill the living organism and to sanitize organic tissues of the organism.  
         [0045]     Preferably, the water fed to the tank is substantially free of dissolved gases and particulate matter. Accordingly, pursuant to an additional feature of the present invention, the method further comprises filtering and degassing the water prior to the feeding of the water to the tank. The degassing of the water may include accelerating the water flow to create micro-sized gas bubbles and bursting the bubbles. The accelerating of the water flow may more particularly include directing the water through a Venturi injector. The bursting of the bubbles may more particularly include impacting the water against a barrier.  
         [0046]     The method preferably also comprises automatically monitoring the water in the tank to detect inertial or transient cavitation. The status of inertial of transient cavitation in the water in the tank may be displayed for inspection by an operator. The generating of the ultrasonic pressure wave vibrations is terminated in the event that cavitation is detected occurring within the water in the tank. This termination may be automatic or initiated by an operator in response to the display alert as to the existence of cavitation in the tank water.  
         [0047]     The generating of ultrasonic pressure wave vibrations may include sweeping a frequency of the ultrasonic pressure wave vibrations.  
         [0048]     Pursuant to additional features of the present invention, the further comprises removing the killed organism from the tank, thereafter delivering disinfectant and water to the tank, and thereafter inducing ultrasonic cavitation in the water and disinfectant in the tank. The inducing of the ultrasonic transient cavitation may include generating full-wave compression and rarefaction cycles at an ultrasonic frequency in the water and disinfectant in the tank. The inducing of the ultrasonic transient cavitation may further include sweeping the frequency.  
     
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0049]      FIG. 1  is an overall system block diagram outlining functional interrelationships among three major elements of a human and land-animal debridement and wound-therapy supine treatment apparatus, which may incorporate a device for killing fish and sanitizing fish tissue in accordance with the present invention.  
         [0050]      FIG. 2A  is a graph of a pulsed waveform used for iterative stable cavitation control in a method and apparatus for treating fish with ultrasonic pressure wave energy, showing a fully rectified wave portion.  
         [0051]      FIG. 2B  is a graph of another pulsed waveform used for iterative stable cavitation control in a method and apparatus for treating fish with ultrasonic pressure wave energy, showing a half-wave rectified wave portion.  
         [0052]      FIG. 3  is a plan view of a two-tank fish farm with an ultrasonic therapy installation.  
         [0053]      FIG. 4  is an elevational view of the two-tank fish farm with ultrasonic therapy installation shown in  FIG. 3 .  
         [0054]      FIG. 5  is a schematic front elevational view of a louvered barrier shown in  FIGS. 3 and 5 .  
         [0055]      FIG. 6  is a side elevational view of the louvered barrier of  FIG. 5 .  
         [0056]      FIG. 7  is an overall system block diagram outlining functional interrelationships of an ultrasonic apparatus for rendering fish instantaneously unconscious and inducing continued insensibility until death supervenes and for concomitantly sanitizing fish tissue, in accordance with the present invention.  
         [0057]      FIG. 8A  is a graph of a continuous peak amplitude adjustable ultrasonic waveform which, when used in conjunction with submicron filtered and degassed water, will apply repeatable subaqeuous ultrasonic compressional and rarefactional pressures sufficient in amplitude to render fish instantaneously unconscious, to continue fish insensibility and concomitantly sanitize fish tissue until death supervenes.  
         [0058]      FIG. 8B  is a graph of a continuous ultrasonic waveform which when used in conjunction with municipal tap water will, following fish slaughtering and sanitization, effect fish tank decontamination.  
         [0059]      FIG. 9  is a plan view of a portion of the two-tank fish farm of  FIG. 3 , configured to be a stand-alone device or apparatus for fish slaughtering and tissue sanitization in accordance with the present invention. 
     
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS  
       [0060]      FIGS. 1-6  illustrate an apparatus for treating fish with ultrasonic pressure waves for wound treatment purposes. The apparatus of  FIGS. 1-6  may incorporate a fish killing and sanitizing functionality described hereinafter with reference principally to  FIGS. 7-9 .  
         [0061]     The following operational description of a wound treatment apparatus applies to human and animal configurations of the apparatus. A configuration of the apparatus for the treatment of fish need not include provision for handling disinfectant in a therapy tank but will include all other operational features plus some additional features necessary to address the needs of fish farming.  
         [0062]      FIG. 1  illustrates a control microcomputer or microprocessor  24  by which means an operator can cause the three processes associated with open-wound ultrasonic therapy to function, as needed. Microcomputer  24  has a control panel (not separately designated) includes an illuminated touchpad  12  for activating the wound treatment apparatus. Another illuminated touchpad  14  initiates a “one time” on-site calibration cycle. A liquid crystal display (LCD)  16  displays all relevant information and operator instructions. A further touchpad  18  is used to initiate a “start selected sequence” routine. Yet another touchpad  20  initiates a “stop selected sequence” routine. A sequencer touchpad  22  is accessed by microcomputer or microprocessor  24  to assist the operator in initiating the required operation.  
         [0063]     The wound treatment apparatus described herein operates in either a manual or an automatic operational mode and either mode is selectable at the operator&#39;s choice.  
         [0064]     Sequencer touchpad  22  runs the LCD  16  through a menu so the operator can make selections as required. The menu is set forth in the normal sequence of operation, i.e., therapy tank fill, wound debridement/cleaning, wound healing, decontamination/auto therapy tank drain, therapy tank drain, fish conditioning and fish excrement removal. Each of these operations, other than therapy tank fill, may be taken in the operator preferred order, e.g., if for humans and animals the operator wanted to disinfect the therapy tank before wound therapy, this is possible but the microcomputer  24  will instruct the operator not to install the patient and will empty the therapy tank at the completion of the automatic decontamination cycle time.  
         [0065]     Before the wound treatment apparatus can be used for routine therapy treatment it must first be calibrated onsite. When a therapy tank  26  ( FIG. 1 ) is filled the first time, the operator activates the system by depressing the illuminated “ON” touchpad  12 . This activates all electronic circuits but stops ultrasound transmission to the therapy tank  26  by opening a switch K 7  to disable an amplifier A 3 . The operator, by means of the sequencer touchpad  22  and LCD  16  selects from the menu option “THERAPY TANK FILL.” Microcomputer  24  then asks the operator via LCD  16  to select “AUTO, (FILL).” After making the required selections the operator is instructed by microcomputer  24  via LCD  16  to depress the “START” touchpad  18 . Microcomputer  24  closes a switch K 4 , which energizes a solenoid S 3  and closes a drain  28 . Then a switch K 6  is closed, which energizes a solenoid S 2  and commences aerated tub fill. The therapy tank fill components are turned off when a preset level is reached as determined by a sensor D 2 .  
       Calibration  
       [0066]     Until microcomputer  24  has conducted its first on-site calibration, it will only respond to an instruction to fill the therapy tank  26 . Microcomputer  24  will tell the operator via LCD  16  why and ask the operator to depress calibration (CAL) touchpad  14 . The calibration cycle is fully automatic and operates as follows.  
         [0067]     An initial step in an iterative control technique is to test the number of full sinusoidal cycles of equal amplitude ultrasonic compressional and rarefactional pressure waves needed to stimulate inertial or transient cavitation in water. This is accomplished by running ten discrete sets of tests of which the longest and the shortest number of cycles are discarded and the average number of cycles is calculated from the remaining eight tests.  
         [0068]     This average number of cycles is the pulse repetition period, i.e., the time from the beginning of one pulse to the beginning of the next. There is no ultrasound “off” time in this pulse repetition period since it is made up of two different pulse types, one immediately following the other. The pulse duration (PD) is the length of time required for the first type pulse to occur and is equal to the period times the number of sinusoidal cycles in the pulse. The duty factor is the fraction of time that the first type pulse is on and consists of full sinusoidal compressional and rarefaction pressure waves. The balance of the pulse repetition period is occupied by the second pulse type, which consists of half sinusoidal (rectified) compressional pressure waves  
         [0069]     The iterative stable cavitation control technique consists essentially of decreasing the above-defined duty factor from 0.8 in increments of 0.1, for example, until the setting is reached where it takes transient cavitation 15 minutes or more to manifest itself, whereupon, the duty factor is reduced, for example, by an increment of 0.1 to provide a safety margin.  
         [0070]     The above iterative control technique is conducted with the average time to transient cavitation calculated from the above ten discrete sets of tests corresponding to the duty factor 1.0 and using the precision microcomputer clock as the determinant for setting the trial duty factors where it takes transient cavitation 15 minutes or more and whose value and increments are adjusted from tank to tank location to suit water quality.  
         [0071]     Upon completion of the above calibration cycle the microcomputer  24  through its LCD  16  confirms that stable cavitation is in effect. Thereafter, the calibrated ultrasound wave configuration is transmitted continuously while the 15-minute “patient” cleaning, wound debridement or wound healing therapy is in progress.  
         [0072]     In an example of a wave configuration arrived at via the above-described iterative calibration technique, the duty factor is 0.4, with full-wave rectification, the applied frequency is 60 kHz, and the pulse repetition period (PRP) is 15 seconds. Then the number of alternate compressional and rarefaction cycles is (15×60,000×0.4)/2 or 180,000. For a duty factor of 0.6, the number of alternate compressional and rarefaction cycles is 270,000. The number of follow-on compressional half-cycles is 15×60,000×0.6 or 540,000 and, for a duty factor of 0.6, the number of alternate compressional and rarefaction cycles is 360,000.  
         [0073]     After the 15-minute therapy period is completed, or transient cavitation is detected, the microcomputer  24  shuts down the ultrasound for a time period sufficient for cavitation to dissipate, whereafter therapy can be resumed for another 15-minute time period, and so on.  
         [0074]     This calibration cycle is more likely a one-time event necessary upon device site installation because water quality varies widely depending on geographical location.  
         [0075]     The final waveform resulting from this calibration at a particular location is placed into the memory of microcomputer  24  and is applied for all subsequent device activations at this particular site location.  
         [0076]     The presence or absence of inertial or transient cavitation is determined by a signal from a PZT probe X 1  ( FIG. 1 ) situated in close proximity to a transducer T 1  and in combination with an appropriately configured detection circuit  30 . PZT probe X 1  generates a signal fed to microcomputer  24 , which manages all associated signals, system components and processes.  
         [0077]     Operator control over microcomputer  24  is provided by a control unit  10  including LCD component  16 , which are situated on or near wound treatment therapy tank  26 .  
         [0078]     Microcomputer  24  induces the energization of transducer T 1  with a full-wave ultrasonic waveform alternating with a rectified alternating waveform, defined by parameters selected during the calibration process as discussed above. This ultrasound generation method suppresses inertial and transient cavitation. The system generates bubbles at the applied frequency and compresses the bubbles so that they are smaller than their resonant size at the applied frequency, thereby prolonging stable cavitation.  
         [0079]     Because vibrating bubble-to-bubble interaction causes bubbles to assume a non-spherical shape, their vibratory response is non-sinusoidal and therefore contains harmonics and sub-harmonics of the applied frequency. A limitation of the above-discussed prior-art human patient cleaning device was the 30 kHz applied frequency because its third sub-harmonic 10 kHz, proved detectable by all immersed human patients through conduction of the 10 kHz subharmonic by their bony prominences to their inner-ear, some patients finding the noise either irritating or intolerable. For the prior human patient cleaning device, lowering the applied intensity served to decrease the amplitude of the third sub-harmonic which lowered the noise to an acceptable level in most but not all cases. This necessary lowering of intensity proved to be at the expense of cleaning process effectiveness for the patient.  
         [0080]     The present apparatus has removed this limitation by increasing the applied frequency to 60 kHz for human and animal exposure and therefore its third sub-harmionic to 20 kHz, which is above the threshold of human hearing. The detection circuit  30  may also employ harmonics for detection of stable cavitation.  
         [0081]     For fish treatment, the applied ultrasonic frequency is lowered to 30-kHz, because the frequency detection capability of farm-raised fish is, at the highest, in the low hundreds of Hz.  
         [0082]     The present apparatus provides four levels of intensity, one for decontamination at more than 5 W/cm 2  SPTP, the second for cleaning and open-wound debridement at 3 W/cm2 SPTP (maximum), the third for wound healing at 1.5 W/cm2 SPTP (maximum), and the fourth for fish conditioning at 0.5 W/cm2 SPTP.  
         [0083]     The 30 or 60 kHz applied frequency is swept up to +/−5 kHz at 120 Hz to provide the likelihood of increased microorganism kill.  
         [0084]     The limitation exhibited by 1-mHz- and -above hand-held therapy device is its inability at 0.1-0.5 W/cm2 intensity to stimulate any form of cavitation in the water thus enabling ultrasonic pressure waves to penetrate a human patient&#39;s body without attenuation, thereby exposing nucleation sites within the patient to cell damage and free radicals from inertial or transient cavitation. This limitation of these hand-held devices can only be removed by lowering their applied frequency and increasing the acoustic intensity of the devices. There&#39;s no better example than the 1 mHz hand-held therapy device for demonstrating that water&#39;s reaction to ultrasonic pressure waves may have unanticipated major harmful effects on the desired therapeutic clinical result and that reliance on first-order, open-ended controls to effect stable cavitation may only serve to increase the risk of cell damage due to the non-visible presence of inertial or transient cavitation within the human patient&#39;s body.  
         [0085]     An advantageous element of the present apparatus is an ability to differentiate between occurrences of stable and inertial or transient cavitation within contained tap water within a wound-therapy tank  26 . An inertial or transient cavitation detection signal always overrides the stable cavitation detection signal so that microcomputer  24  can suppress or maintain inertial or transient cavitation depending on the required mode of operation. The location of PZT probe X 1  of detector circuit  30  is in-line with a face  32  of transducer T 1  at the highest intensity within the wound-therapy tank  26 . In response to a signal from PZT probe X 1 , microcomputer  24  displays on LCD  16  the cavitation status within the water contained within the wound-therapy tank  26  at all times during operation.  
         [0086]     PZT probe X 1  and detection circuitry  30 , inter alia, overcome the limitation of prior human patient cleaning devices in their inability to detect inertial or transient cavitation and to thereby maintain stable cavitation suitable for wound-therapy.  
       Decontamination  
       [0087]     The need is recognized for disinfection of a tank  26  used for ultrasound wound treatment. After completion of a wound-therapy procedure in therapy tank  26 , the tank must be decontaminated from pathogens shed by the patient or subject. A number of microorganisms have been found to withstand hot-water temperatures and chemical disinfectants, which suggests that chemical means alone are not 100% effective. Also, experimental data suggests that ultrasound in the low-kilohertz frequency range is capable to some measure of inactivating certain human disease agents that may reside in water. This experimental ultrasound data states that the human pathogens tested were selected on their normal routes of infection, for example, skin or intestinal tract, or their structural similarities to such agents, which would make them likely candidates of whirlpool or hot tubs.  
         [0088]     In an experiment, ultrasound killed within 1 hour a variable percentage of the following microorganisms: bacteria ( Pseudomonas aeruginosa, Bacillus subtilis Escherichia coli ), fungus ( Trichophyton mentagrophytes ) and viruses (feline herpes virus type 1; this sub-family also includes the human herpes viruses, herpes simplex virus types 1 and 2). This experiment concluded that 100% microorganism killing was a dose-effect dependent on time of exposure and level of ultrasound intensity but the mechanism of microorganism “kill” appeared to be inertial or transient cavitation.  
         [0089]     This microorganism “kill” principle appeared to be the high forces and high temperatures associated with inertial or transient implosions which can disintegrate cell walls and membranes of bacteria and certain enveloped virus but only in the immediate vicinity of these micro-sized implosions. Because an apparent defense mechanism of pathogens is to gather at the antinodes of a constant frequency ultrasonic wave where the amplitude of the ultrasound pressure wave is at a minimum, the present apparatus employs a rapid frequency-sweep modality which serves to oscillate the location of the antinodes in space thereby exposing the microorganisms to an increased number of cavitation implosion events.  
         [0090]     Experimental data reveals that ultrasonic cavitation enhances the effect of different antibiotics and disinfectants. Clearly, disinfectant plays no part in the deactivating of pathogens exposed to the high forces and temperatures created by cavitation implosion events. Reasons for the synergism of water, ultrasound and disinfectant having an apparently enhancing germicidal effect over water and disinfectant alone are largely unknown. Since experiments have demonstrated acoustic pressure waves used in conjunction with disinfectant does exhibit an increased germicidal effect, the synergism hypothesis is that like vibrating bubbles the pathogens are subjected to alternating compression and rarefaction ultrasonic pressure waves. Since the pathogen&#39;s internal contents are normally equalized in pressure corresponding to ambient pressure, in the presence of a rarefaction pressure wave an enveloped pathogen expands in size from internal pressure because of the absence of balancing external pressure. On the following ultrasonic compressional pressure wave the enveloped pathogen is squeezed to a size smaller than normal, further increasing the pressure on the inner contents. The oscillatory stress pattern on a pathogen&#39;s envelope could be repeated up to 30,000 to 60,000 times each second. It is hypothesized that these many positive to negative stress inversions may cause cell-wall fatigue which in turn creates fissures or even fractures in a cell-wall or membrane which open upon rarefaction pressure cycles, thereby exposing the microorganism&#39;s inner contents and then close shut on the compression pressure cycle.  
         [0091]     It is also hypothesized that if the medium surrounding the pathogen was water these stress inversions on the pathogen might be survivable for longer time-periods, but when the medium is disinfectant, as the fissures or fractures open on the rarefaction pressure cycle exposing the pathogen&#39;s inner contents to disinfectant, the following compressional pressure cycle forces the disinfectant into the cell&#39;s interior, thereby killing the cell.  
         [0092]     The present apparatus exhibits a decontamination cycle employing a combination of water, ultrasonic pressure waves, and disinfectant in order to secure disinfection within a shorter time period than is possible with ultrasound and water alone or disinfectant alone, with the goal of taking less time to effect disinfection than current hospital procedures, which range typically from 12-30-minutes. The required disinfectant should exhibit a surface tension approaching that of water (72 dyne/cm) and a viscosity approaching that of water (0.01 poise) and exhibit germicidal action against the microorganisms listed above and those microorganisms appropriate to animals, and be non-flammable.  
         [0093]     In fish farming applications, there is a difficulty with containing disinfectant in the ultrasonic therapy tank. Therefore disinfectant is not used to kill fish microorganisms. Instead, the necessary full ultrasonic dose effect (time and ultrasonic intensity) applicable to transient cavitation 100% microorganism kill is used.  
         [0094]     In order to increase the spectrum of microorganism kill, it is intended that all patient germicidal cycles employ the wound therapy pulsed waveform with its duty factor set to low values (less than 0.4), sufficient to induce transient cavitation and the collapsing of very small bubbles.  
         [0095]     Because decontamination is accomplished by the use of transient cavitation and not stable cavitation, the apparatus includes a number of patient precautionary or protection measures. The decontamination cycle is under microcomputer control, which dictates the following operational sequence.  
         [0096]     By means of touchpads  12 ,  18 ,  22 , etc., therapy tank  26  is automatically filled to preset levels and also emptied automatically (except in the case of fish). Ultrasonic decontamination cannot be accessed until wound treatment has been conducted and operator confirmation of patient or subject removal from the therapy tank  26 . Microcomputer  24  determines readiness for wound treatment by noting that the wound treatment preset tank fill level and automatic water shut off is completed. After termination of the preset 15-minute wound treatment time, microcomputer  24  informs the operator via LCD  16  that therapy tank decontamination can take place and provides the necessary touchpad instructions via the LCD. The instructions include the appropriate decontamination information and an instruction requiring mandatory patient removal from the therapy tank  26  before decontamination can be initiated and requires touchpad confirmation of patient removal to be confirmed to microcomputer memory. After confirmation of patient removal, microcomputer  24  adds a specific volume and dilution of tap water and disinfectant to the therapy tank  26  then activates ultrasonic full-wave, equal-amplitude compressional and rarefaction and half-cycle compressional pressure waves sufficient to cause transient cavitation for the preset decontamination time period after which the microcomputer automatically switches off the ultrasound. For a fish decontamination cycle, disinfectant may not be employed due to the difficulty of containing disinfectant in the ultrasonic therapy tank. During the decontamination time period, audible and visual annunciators including a flashing LCD display are active, signifying an “operator precautionary” condition.  
         [0097]     After decontamination is completed, microcomputer  24  automatically drains tank  26  and requests via LCD  16  that the tank be rinsed with tap water and then dried with germ-free cloths or a thermal blow drier. Microcomputer  24  disconnects the system from electrical power after a preset time period.  
         [0098]     Microcomputer  24 , using its internal precision clock, synchronizes with a 30 or 60 kHz oscillator O 1  to time an interval from a closing of switches K 1  and K 7  and an activating of amplifier A 3  to a signaling of an adjustable-gain amplifier A 2  by PZT detector X 1  that transient or inertial cavitation has taken place, at which time the microcomputer places the resulting time into temporary memory then repeats the process for a total of ten times before calculating the average pulse repetition period. Using the average pulse repetition period, the calibration cycle (or program) next requires microcomputer  24 , using its internal precision clock, to synchronize the 30 or 60 kHz oscillator O 1  with a full-wave rectifier R 1  to form a 0.8 duty factor pulse-train (see  FIG. 2A ) and then to close switches K 1  and K 7  which activates amplifier A 3  until PZT detector XI signals the adjustable-gain amplifier A 2  that transient or inertial cavitation has taken place, at which time the microcomputer compares the elapsed time from the activation of amplifier A 3  until to the signaling by amplifier A 2  that transient cavitation has occurred for the required time of 15 minutes.  
         [0099]     Microcomputer  24  automatically resets the duty factor to a lower value and continues as described above until a duty factor value is attained that results in the required time of 15 minutes. The duty factor may be reset in increments of less than 0.1.  
         [0100]     PZT detector X 1  feeds both a 10 or 20 kHz acceptor circuit A 4  and a 10 or 20 kHz rejector circuit R 2  which feed an adjustable gain narrow-band sub or harmonic amplifier A 1  and the adjustable-gain broadband amplifier A 2  whose outputs are fed to the microprocessor. Acceptor circuit A 4  and rejector circuit R 2  may employ harmonics or sub-harmonics.  
         [0101]     From the amplifiers A 1  and A 2  outputs the microprocessor  24  determines the required pulsed waveform needed to arrest inertial or transient cavitation for a minimum time period of 15 minutes (or other suitable time selectable by the operator).  
         [0102]     After the required pulsed waveform has been determined, microcomputer  24  places the defining parameters of the determined pulse waveform into an internal memory. Those parameters are used thereafter for all open-wound therapy purposes at the particular installation site.  
         [0103]     The operator can empty the therapy tank  26  either by following instructions displayed on LCD  16  or by depressing illuminated “ON” touchpad  12 . Either action opens switch K 4 , thereby de-energizing solenoid S 3  to open drain  28 . Subsequently depressing the “illuminated” ON touchpad  12  removes all electrical power from the apparatus including touchpad illumination.  
         [0104]     Upon completion of the onsite calibration cycle, the apparatus is ready for routine open-wound therapy treatment or, if required, intact tissue “patient” cleaning (see  FIG. 2A ) for the cleaning and therapy pulsed waveforms.  
         [0105]     There are four intensity levels of ultrasonic transmission: (1) decontamination triggered or activated by switch K 1 , (2) wound debridement/cleaning, triggered or controlled by operation of a switch K 2 , (3) wound healing, which is triggered or activated by operation of a switch K 3 , and (3) fish conditioning, which is initiated by operation of a switch K 8 .  
         [0106]     There are three modes of ultrasonic transmission: (1) continuous, which is reserved for the decontamination cycle, (22) pulsed at a duty factor greater than 0.4 for the decontamination cycle, and (3) pulsed for the absence of inertial or transient cavitation which is reserved for the wound debridement, wound healing and fish conditioning cycles.  
         [0107]     Microcomputer  24  alternately enables and disables rectifier R 1  using either a duty factor of less than 0.4 (enable), or 1.0 (disable) for the decontamination mode, and only enables rectifier R 1  for wound debridement, wound healing and fish conditioning (see  FIG. 2 ).  
         [0108]     The duty factor (less than 0.4) is determined by microcomputer  24  in a fashion similar to that described above, with the criteria being the lowest duty factor that stimulates continuous transient cavitation due to a majority of compressive pressure waves that collapse very small bubbles.  
         [0109]     During a decontamination process preferably used in connection with the treatment of humans and animals but probably not fish, microcomputer  24  holds off amplifier A 3  by keeping switch K 7  open until it has completed the following actions: (i) the therapy tank is filled to the preset control level detected by sensor D 2 , (ii) the ambient-air input normally fed through the Venturi for therapy tank filling is replaced by disinfectant by closing switch K 5  which energises solenoid S 1 , and (iii) the microcomputer clock is set to deliver the preset dilution of disinfectant necessary to effect the required sonic germicidal action based on the volume of water contained by therapy tank  26  up to its overflow port and beyond, if necessary (experiment). Microcomputer  24  then closes switch K 6 , which energizes solenoid S 2  so that the velocity of the water supply causes a Venturi I 1  to suck in disinfectant until the microcomputer shuts down the water supply and disinfectant by de-energising solenoids S 1  and S 2 . Venturi I 1  is an injector posed along a feed pipe  34  proximate to a barrier formed by a wall of the pipe, for instance, at a 90-degree elbow-type bend  36  in the feed pipe. Venturi injector I 1  is operatively connected to solenoid valve S 1  on an upstream side for introducing air into a water stream flowing along feed pipe  34 . Venturi injector I 1  is alternately coupled to a disinfectant reservoir  38  via solenoid valve S 1 , whereby the injector introduces a disinfectant into a water stream flowing along feed pipe  34 .  
         [0110]     Microcomputer  24  then delivers 30 or 60 kHz ultrasound at an intensity in excess of 5 W/cm2 SPTP for the pre-set decontamination time period as monitored and controlled by the microcomputer clock. Upon completion of the decontamination cycle, and in the auto mode, the microcomputer opens the drain  28  by opening switch K 4  which de-energizes solenoid S 3 . Microcomputer  24  then follows its shut down procedures prior to disconnecting from electrical power.  
         [0111]     The present apparatus as used for treating fish utilizes existing technology for general water-quality maintenance in fish holding tanks  40  and  42  ( FIGS. 3 and 4 ) such as the requisite number of sequential rotating water jets (not shown) situated on a tank&#39;s bottom surface  44 ,  46  necessary to sweep all fish excrement from the tank bottom surfaces into a drain return  78  situated at the lowest point on the tank bottom  44 ,  46 . The fish excrement particulate is then sucked into and is retained by filters (which are removable and cleanable) by means of a bi-directional pump/motor assembly  58  including a motor  100 , a pump  102 , and pair of filters  104  and  106   
         [0112]     Additionally,  FIGS. 4 and 5  illustrate several methods for providing results beneficial for fish raised in fish-farming facilities, for example, the water in which the fish swim can be recirculated continuously and irradiated with high intensity ultrasound for decontamination purposes. In this way water-borne fungi, parasites (e.g., lice) and microorganisms can be destroyed through transient or inertial cavitation without the intervention of disinfectants (without a decontamination cycle).  
         [0113]     At selected time periods, daily or two or three times weekly, fish farmed in a system having two or more holding tanks  40 ,  42  can be recirculated from one tank  40 ,  42  to another  42 ,  40  and while passing through an ultrasound section or therapy tank  48  can be irradiated with low intensity ultrasound to effect improvement in blood circulation and fat reduction (wound healing cycle). When used in this manner, ultrasound treatment can be viewed as equivalent to preventative medicine because fish reared in holding tanks are denied vigorous normal “outside activity” which helps to keep them healthy.  
         [0114]     As depicted in  FIG. 3 , tank  48  includes a plurality of transducers  66  provided in a bottom surface  68 . A tap  72  is provided at an upper end of the tank  48 , while a protective mesh or screen  70  may be provided in tank  48  above transducers  66 . A walkway  74  is provided about tanks  40 ,  42 , and  48 .  
         [0115]     Fish in a distressed or contaminated condition can be isolated from healthy fish and treated separately and collectively in the integral ultrasonic therapy tank  48  for removal and destruction of pathogen, fungal and ectoparasitic infection (wound debridement/cleaning cycle). After treatment these fish are isolated by moving them to a separate quarantine tank  76  from which they are periodically ultrasonically treated and not returned until fully cured to the general fish population.  
         [0116]     When a two or more holding tank system is in need of maintenance or removal of solid waste excrement from a given tank, then fish can be transferred from one tank  40 ,  42  to another  42 ,  40  while this is accomplished. Upon completion, the tank  40 ,  42  that received maintenance is refilled and the fish transfer accomplished as needed (tank cleaning cycle).  
         [0117]     Louvered barriers  50  and  52  are provided to limit the fish movement between tanks  40 ,  42  and the ultrasonic therapy tank  48 . As depicted in  FIGS. 5 and 6 , louvered barriers  50  and  52  each include a welded frame  60  and a movable water-sealing louver or door  62  that have a width sufficient to allow free passage of fish. Door  62  is made of a light-weight sound-absorbing material. These louvered barriers  50 ,  52  can be alternately opened and closed manually via a lever or knob  64  that turns a worm  63  meshing with a wheel  65 . Alternatively and preferably, louvered barriers  50 ,  52  are operated by automatic means controlled by a microcomputer controller  56 . The following description assumes microcomputer automatic control.  
         [0118]     As required, a bi-directional circulating motor, pump and filter  58  provide a slow-moving water flow from one tank  40 ,  42  to another  42 ,  40 . In the decontamination cycle, the louvered barriers  50  and  52  are adjusted sufficiently not to interrupt water-flow but closed sufficiently to prevent fish entry before the high-intensity ultrasound can be activated.  
         [0119]     The microcomputer  56  through its LCD requires the operator to remove all fish from the ultrasonic therapy tank  48 , which the operator must confirm through appropriate keypad entry. High-intensity ultrasound is then activated to generate transient or inertial cavitation. This operational mode can be sustained 24 hours daily, 7 days weekly or until a different operational cycle is selected using the keypad. However, before such action is undertaken, the microcomputer  56  switches off the high-intensity ultrasound.  
         [0120]     As required, the bi-directional motor, pump and filter  58  provide a slow-moving water flow from one tank  40 ,  42  to another  42 ,  40 . With the wound healing cycle selection, the ultrasonic-tank water tap is activated to provide aerated water into the ultrasonic tank area and remains activated until the fish “conditioning cycle” is completed. When sufficient aeration has been provided, the low-intensity ultrasound is activated and the louvered barriers  50  and  52  are opened fully to permit free fish entry and exit from one tank  40 ,  42  to another  42 ,  40 . Manual participation of the operator is required to move all fish from one tank to another to ensure that all fish are sonicated.  
         [0121]     This fish condition cycle is completed by the operator depressing the stop cycle touchpad. This shuts down the generation of ultrasonic pressure waves in therapy tank  48 . The microcomputer  56  through its LCD asks the operator whether all the fish have been removed from the ultrasonic therapy tank, which the operator confirms through appropriate keypad entry. The microcomputer  56  then adjusts louvered barriers  50  and  52  sufficiently not to interrupt water flow but closed sufficiently to prevent fish entry. An adjustable automatic timer (part of the microcomputer  56 ) is provided to automatically shut down this cycle in the event of operator absence.  
         [0122]     As required, the bi-directional motor, pump and filter  58  must be switched off and the louvered barriers  50  and  52  tightly closed by a pneumatic pump  80  that inflates a tubular sealing member  81 . The operator depresses the microcomputer stop cycle touchpad to accomplish this. The LCD will ask the operator “what&#39;s next.” The operator uses the sequencer touchpad  22  ( FIG. 1 ) to select and then start the wound debridement/cleaning cycle. This cycle is almost identical to that used for humans, except that the microcomputer  56  simultaneously fills and drains the ultrasonic therapy tank  48  until the necessary aerated water exchange is effectuated after which a drain  54  is closed and the ultrasonics switched on. The distressed or contaminated fish are placed in the therapy tank  48  for the automatically prescribed treatment time period. Thereafter the microcomputer LCD instructs the operator to remove the fish to quarantine tank  76  and requires confirmation from the operator. This treatment cycle is to be repeated periodically every few days until the fish(es) in question is judged healed and free from infection.  
         [0123]     Following the current fish decontamination cycle, the operator uses the touchpad to initiate a high ultrasound intensity decontamination cycle in therapy tank  48 , after which the microcomputer  56  switches off the ultrasound and drains the tank. After tank draining, the microcomputer asks the operator if the debridement/cleaning cycle is to be repeated. If the answer is in the affirmative, the microcomputer  56  refills the tank, etc., and proceeds as before. If not, the microcomputer  56  adjusts louvered barriers  50  and  52  sufficiently not to interrupt water flow but closed sufficiently to prevent fish entry. In debridement/cleaning cycle, it is to be noted that with every therapy tank emptying the opening of louvered barriers  50  and  52  will lower the water level in the fish holding tanks  40  and  42 . This level depletion will be automatically made up by automatic water level sensing floats (not shown).  
         [0124]     At the next operator cycle selection, other than debridement/cleaning cycle, the microcomputer  56  will switch the water circulating motor, pump and filter  58  back on.  
         [0125]     In the event that solid excrement waste from the fish tank needs to be removed from a holding tank, the following procedure is performed. The operator, using the touchpad sequencer, selects “excrement removal, Tank B” (referring to tank  42 ), for example, and starts the process. The microcomputer  56  fully opens louvered barriers  50  and  52  to allow free passage of the fish and subsequently reverses the flow of the recirculating pump to assist in fish transfer from tank  42  to tank  40 . After all the fish have been transferred then the operator, using the touchpad, alerts the microcomputer  56  which then fully closes barrier  50  while barrier  52  is left open and the circulating pump is again reversed which empties tank  42  to “drain” after which the circulating pump is switched off.  
         [0126]     After excrement removal, tank  42  is refilled, barrier  50  is opened and the microcomputer  56  switches the circulating pump back on, allowing the fish to return with assistance from tank  40  to tank  42 , for example.  
         [0127]     As part of the water high intensity ultrasound decontamination cycle the microcomputer  56  automatically reverses the water flow commensurate with time necessary to drain the holding tank in question.  
       Fish Killing and Tissue Sanitization Apparatus  
       [0128]     The following operational description is of a stand-alone ultrasonic fish slaughtering and tissue sanitization apparatus applicable to all fish species that experience accelerated mortification when subjected to subaqueous ultrasonic pressure waves having frequencies in the range 20-60 kHz and peak amplitude acoustic pressure waves in the range 75 Pa-300 kPa. The apparatus may be used with a dedicated tank  26 ′ shown in  FIG. 9  or in conjunction with the wound treatment tank  26  of  FIGS. 3 and 4 . Although the discussion below is directed mainly to tank  26 ′, it should be understood that the same procedure could be used using tank  26  of  FIGS. 3 and 4 . In the latter case, the functionality described herein with reference to  FIG. 7  may be added to the functions described above with reference to  FIG. 1 . Thus, the apparatus of  FIG. 1  may be modified to incorporate the functionality of the apparatus of  FIG. 7 .  
         [0129]      FIG. 7  illustrates a control microcomputer  24 ′ by which an operator can initiate, control, modify, and terminate processes for fish slaughtering and tissue sanitization. Microcomputer  24 ′ has a control panel  10 ′ and includes an illuminated “on” touchpad  12 ′ for activating the invention&#39;s fish slaughtering and tissue sanitizing apparatus. An illuminated calibration touchpad  14 ′ initiates the factory/onsite calibration cycle for particular fish species slaughter/sanitization calibration.  
         [0130]     A liquid crystal display (LCD)  16 ′ displays all relevant information and operator instructions. A further start touchpad  18 ′ is used to initiate a “start selected sequence” routine. An abort touchpad  20 ′ initiates a “stop/abort selected sequence” routine. A sequencer touchpad  22 ′ is accessed by microcomputer  24 ′ to assist the operator to locate the required process.  
         [0131]     The fish slaughtering and tissue sanitization apparatus described herein operates in either a manual or in an automatic operational mode. Either mode is selectable by the operator.  
         [0132]     Sequencer touchpad  22 ′ runs the LCD  16 ′ through a menu so the operator can identify and initiate selections as required. The menu displayable via LCD  16 ′ is set forth in the normal sequence of processing, that is: 
        fish slaughtering/sanitization tank fill     fish slaughtering/sanitization calibration—supporting initiating LCD instructions     fish slaughtering/sanitization—supporting initiating LCD instructions     fish slaughtering/sanitization tank empty     tank decontamination, empty/fill     tank decontamination—supporting LCD initiating instructions     tank decontamination, empty.        
 
         [0140]     It is to be noted that the three decontamination cycles are included as one automatic process.  
         [0141]     Before the fish slaughtering/sanitization apparatus can be used in the “automatic” mode of operation it must first have been calibrated for the fish species in question. This should be accomplished at an approved factory and/or a properly equipped and approved onsite fish-farm location.  
       Calibration  
       [0142]     In order to effect the calibration process, tank  26 ′ must first be filled with submicron filtered/degassed water. At the end of the calibration cycle, tank  26 ′ must be emptied and refilled with tap water in preparation for the follow-on ultrasonic decontamination cycle.  
         [0143]     An imperative for the fish slaughtering/sanitization calibration process is production of an end product exhibiting the highest market quality as characterized by exemplary external and internal appearance of the slaughtered fish, longer shelf life than available by current methods of slaughter and satisfactory taste.  
         [0144]     Another imperative is that regardless of species, the fish slaughtering/sanitization calibration process must result in all fish of a given species experiencing rapid unconsciousness and continuing insensibility until death supervenes.  
         [0145]     Prior to conducting the following ultrasonic calibration process, the calibrator must be instructed to employ the lowest peak pressure amplitude and the longest possible exposure time that result in accomplishment of the aforementioned marketing and environmental imperatives.  
         [0146]     The rapidity of fish unconsciousness is proportional to the maximum applied peak pressure amplitude while the effectiveness of tissue sanitization is proportional to the length of the exposure time. However, the end values for both criteria should not deviate from the above marketing and environmental imperatives. Fish “harvest” weight for all species is a second order effect and should be kept consistent from batch to batch.  
         [0147]     In preparation for slaughter/sanitization calibration by fish species, the apparatus is activated by depressing the ON/OFF touchpad  12 ′ which accesses the microcomputer  24 ′, which by means of LCD  16 ′ assists the calibrator to locate and subsequently complete the required operations.  
         [0148]     The calibrator, using the sequencer touchpad  22 ′, runs the LCD  16 ′, through the menu until reaching the heading “Fish Slaughtering/Sanitization” then through a listing of all fish species for which the apparatus has already been factory calibrated. For each of the listed species, relevant “codes” for the lowest peak pressure amplitude and longest exposure time to effect slaughter/sanitization in the approved manner are displayed by LCD  16 ′.  
         [0149]     In the absence of a desired species&#39; slaughtering/sanitization information, the apparatus is calibrated as follows. The calibrator depresses the calibration touchpad  14 ′, which accesses and displays the “codes” for associated peak pressure amplitudes in the range 75 Pa-300 kPa and then, by means of the sequencer touchpad  22 ′, scrolls up or down the “codes” to locate and select a trial peak pressure amplitude and associated trial time of exposure. Both the peak pressure and exposure time codes will “flash” upon reaching the LCD display  16 ′.  
         [0150]     To lock in the “flashing” trial peak pressure amplitude selected, the calibrator depresses the start touchpad  18 ′, which causes the stepper motor M 1 ′ to drive the auto transformer T 2 ′ voltage selector to the encoder E 1 ′ position which corresponds to the calibrator&#39;s peak pressure amplitude selection.  
         [0151]     To lock-in the “flashing” associated trial time of exposure selected, the calibrator depresses the start touch pad  18 ′ for a second time which causes microcomputer  24 ′ to correlate the two trial requirements.  
         [0152]     After correlation has been completed, microcomputer  24 ′, by means of LCD  16 ′, will instruct the calibrator to depress the start touchpad  18 ′ for a third time in order to initiate the trial slaughtering/sanitization process.  
         [0153]     Following an unsuccessful trial slaughtering/sanitization calibration, the calibrator can, by depressing the abort touchpad  20 ′, abort, (delete) all data-entries associated with an unsuccessful trial.  
         [0154]     However, upon successful completion of a repeated trial, microcomputer  24 ′ by means of LCD  16 ′ instructs the calibrator to depress the calibration touchpad  14 ′ to enter the selected trial peak pressure amplitude and associated trial time of exposure data into microcomputer  24 ′ for which entry it assigns a “code” then places it into the memory (not separately shown) of microcomputer  24 ′ to end the calibration process.  
         [0155]     Thereafter the calibrator must keep either a separate fish species identification “Code” listing for such field trial slaughtering/sanitization calibration processes or arrange for field or factory reprogramming of microcomputer  24 ′ by means of appropriate programming apparatus (not shown) utilizing the programming input connector C 1 ′ located on control unit  10 ′.  
         [0156]     The final selected peak pressure amplitude and associated time of exposure data, entries from the above trial calibrations for a previously un-calibrated fish species, as implied, are placed into the memory of microcomputer  24 ′ and are applied for all subsequent device activations at the appropriate “Fish Slaughtering/Sanitization” data-entry site location.  
         [0157]     The slaughtering/sanitization calibration process must be carried out using commercially available laboratory quality filtered and degassed water or, water from a municipal supply after its treatment for ozone removal followed by submicron reverse osmosis filtering and degassing techniques equivalent to or superior to those employed by this invention, as illustrated by  FIG. 7 .  
         [0158]     The presence or absence of inertial or transient cavitation in tank  26 ′ will be detected by a signal from a PZT probe X 1 ′ situated in close proximity to a transducer T 1 ′ and in combination with an appropriately configured detection circuit  30 ′. PZT probe X 1 ′ generates a signal fed to microcomputer  24 ′, which manages all associated signals, system components and processes.  
         [0159]     Operator control over microcomputer  24 ′ is provided by a control unit  10 ′ including LCD  16 ′ that is situated on or near the slaughtering/sanitization tank  26 ′.  
         [0160]     Microcomputer  24 ′ induces energization of transducer T 1 ′ with a full-wave subaqueous 30 kHz ultrasonic waveform. The utilization of submicron filtered and degassed water with this ultrasound generation method serves to suppress inertial and transient cavitation permitting virtually the full amplitude of the compressional and rarefaction pressure waves propagated by transducer T 1 ′ to travel the full volume of the slaughtering tank  26 ′ with minimal attenuation which causes the fish-body&#39;s interior tissue to experience the required transient bubble imploding cavitation events.  
         [0161]     Fish will avoid and try to escape from unfamiliar acoustic noise in their vicinity. Because the frequency detection capability of most farm-raised fish is in the low hundreds of cycles of vibration, the chosen “slaughtering/sanitization” frequency of 30 kHz is undetectable by such fish, which serves to overcome their escape reflex.  
         [0162]     With previously calibrated fish species, the present apparatus provides operator selectable ultrasonic slaughtering peak pressure amplitudes and associated times of ultrasonic exposure applicable to the fish species slated to undergo the slaughter/sanitization process.  
         [0163]     For selection of existing fish species controls, the operator first depresses the On/Off touchpad  12 ′, to activate the control unit  10 ′ and microprocessor  24 ′, then, by depressing the sequencer touchpad  22 ′ the operator runs the LCD  16 ′ through the menu until reaching the headline “Fish Slaughtering/Sanitization” and then down to the fish species required whose data, when in the LCD display  16 ′, will start “flashing”.  
         [0164]     To effect automatic fish slaughtering/sanitization the operator first closes the transparent, vented tank lid L 1 ′, then depresses the start touchpad  18 ′ causing microcomputer  24 ′, by means of LCD  16 ′, to advise the operator when the automatic fish laughtering/sanitization process is finished. The operator then raises transparent tank lid L 1 ′ and removes the slaughtered fish for completion of other activities such as fish inspection, packing and shipping.  
         [0165]     During the slaughtering process the 30 kHz applied frequency is swept +/−5 kHz to increase water-borne microorganism infestation kill.  
         [0166]     An advantageous element of the present fish killing and sanitization apparatus is its ability to detect transient and inertial cavitation occurring within the filtered/degassed water in tank  26 ′. A transient or inertial cavitation detection signal from the PZT detector X 1 ′ brings the fish slaughtering/sanitization process to a halt until the water contained by tank  26 ′, has been drained then refilled with fresh submicron filtered degassed water.  
         [0167]     LCD  16 ′ displays the cavitation status within the water contained within the tank  26 ′ at all times during the slaughtering/sanitization processing cycle.  
         [0168]     PZT probe X 1 ′ and detection circuitry  30 ′, inter alia, overcomes the limitation of current ultrasonic irradiation tanks because of its ability to detect inertial or transient cavitation which phenomenon is counter-productive to the effectiveness of the invention&#39;s fish slaughtering and sanitization process.  
       Decontamination  
       [0169]     The need is recognized for disinfection of tank  26 ′ following fish slaughtering. After completion of the slaughtering process and removal of fish from tank  26 ′, tank  26 ′ must be decontaminated from pathogens shed by the fish. A number of microorganisms have been found to withstand hot-water temperatures and chemical disinfectants, which suggests that chemical means alone are not 100% effective. Also, experimental data has shown that ultrasound in the low kilohertz range is capable, to some measure, of inactivating certain pathogens that may reside in water.  
         [0170]     For the tank decontamination cycle, unfiltered municipal water is substituted for filtered/degassed and or reverse osmosis processed water in order to promote the formation of both ultrasonic transient and inertial cavitation which is a necessary part of the decontamination process used for inactivating and killing shed pathogens and parasites.  
         [0171]     The ultrasonic decontamination microorganism “kill” principle depends on the high forces and high temperatures associated with inertial or transient implosions which can disintegrate microorganism cell walls and membranes of bacteria and certain enveloped virus but only in the immediate vicinity of these micro-sized implosions. Because an apparent defense mechanism of pathogens is to gather at the antinodes of a constant frequency ultrasonic wave where the amplitude of the ultrasonic pressure wave is at a minimum, the present apparatus employs a rapid frequency-sweep modality which serves to oscillate the location of the antinodes in space thereby exposing the microorganisms to an increased number of cavitation implosion events.  
         [0172]     Experimental data has revealed that ultrasonic cavitation enhances the effect of different antibiotics and disinfectants. Clearly, disinfectant plays no part in the deactivating of pathogens exposed to the high forces and temperatures created by cavitation implosion events. Reasons for the synergism of water, ultrasound and disinfectant are largely unknown.  
         [0173]     The present fish killing and sanitization apparatus exhibits a decontamination cycle employing a combination of water, ultrasonic pressure waves, and disinfectant in order to secure disinfection within a shorter time period than is possible with ultrasound and water alone or disinfectant alone, with the goal of taking less time to effect disinfection than current procedures, which range typically from 12-30 minutes. The required disinfectant should exhibit a surface tension approaching that of water (72 dyne/cm) and a viscosity approaching that of water (0.01 poise) and exhibit germicidal action against microorganisms appropriate to fish-farming.  
         [0174]     The decontamination cycle is under the control of microcomputer  24 ′ by means of LCD  16 ′ and touchpads  12 ′,  18 ′,  20 ′,  22 ′. Operator intervention, requested via microcomputer  24 ′, dictates the automatic filling of tank  26 ′ to a preset level and then its emptying.  
       Tank Filling for Fish Killing and Tissue Sanization  
       [0175]     To fill tank  26 ′ for fish slaughtering and sanitization, the operator depresses the On/Off touchpad which electrically activates microcomputer  24 ′, then depresses the sequencer touchpad  22 ′ and by its use scrolls LCD  16 ′ up or down until the headline “Fish Slaughtering/Sanitization Tank Fill” is flashing in the LCD display  16 ′. To commence automatic filling of tank  26 ′, the operator depresses the start touchpad  18 ′ which causes microcomputer  24 ′ to initiate the following actions.  
         [0176]     Microcomputer  24 ′ closes relay contact K 3  which energizes solenoid S 3 ′ closing the drain in readiness for tank  26 ′ filling. Microcomputer  24 ′ then closes relay contact K 4 ′, energizing solenoid S 4 ′ to open the flow of water from the municipal water supply. The municipal water supply flows through the activated carbon filter (ACF), removing the chlorine content from the water before routing the water to and through the submicron reverse osmosis filter RO.  
         [0177]     When the level of water in the RO reservoir (not shown) reaches the preset level detected by sensor D 3 ′, microcomputer  24 ′ closes relay contact K 2 ′, energizing pump P 1 ′ to direct the water flow through backflow preventer BP 1 ′ into tee connection T 3 ′ and injector I 1 ′ (or equivalent) posed along a feed pipe  34 ′ proximate to a barrier formed by a wall of the pipe, for instance, at a 90-degree elbow-type bend  36 ′ in the water feed pipe.  
         [0178]     The drop in water pressure that occurs on the exit side of injector I 1 ′ accelerates the water flow and creates microsized gas-bubbles that burst upon reaching the barrier formed by a wall of the pipe, for instance, at a 90-degree elbow-bend  36 ′ in the water feed pipe, where from the occluded gas is released to atmosphere.  
         [0179]     The resulting flow into tank  26 ′ is water from which all particles above submicron size and occluded gases have been removed. Tank  26 ′ continues filling until reaching the preset control level detected by sensor D 2 ′ whereupon microcomputer  24 ′ de-energizes relay K 2 ′ causing the pump P 1 ′ to shut down terminating the submicron filtered and degassed water flow.  
       Fish Slaughtering/Sanitization  
       [0180]     This description assumes the operator has filled tank  26 ′ with submicron filtered and degassed water, as discussed above, and, in accordance with microcomputer  24 ′ instructions communicated by means of LCD  16 ′, has loaded tank  26 ′ with the prerequisite number of fish while observing the precaution (communicated via LCD  16 ′) not to mix fish species unless they have very similar peak pressure amplitudes and times of exposure before closing the transparent vented lid L 1 ′.  
         [0181]     The operator then uses sequencer touchpad  22 ′ to scroll LCD  16 ′ up or down until reaching the headline “Fish Slaughtering/Sanitization” and then to the code and data for the fish species selected for slaughter. In the case of mixed fish species the operator must have first verified that close correspondence of peak pressure amplitudes and exposure times exists. In case of significant mismatch one or another of the species must be removed from tank  26 ′ before slaughter is initiated.  
         [0182]     The operator then depresses start touchpad  18 ′ which initiates the automatic slaughtering/sanitizing process as follows. Microcomputer  24 ′ closes relay K 1 ′ causing stepper motor M 1 ′ to drive the voltage of autotransformer T 2 ′ to the required position of encoder E 2 ′ then closes relay contact K 7 ′ which, by means of oscillator  01 ′ and amplifier A 3 ′, drives the transducer T 1 ′ for the programmed exposure time whereafter relay contacts K 1 ′ and K 7 ′ open to complete the process.  
         [0183]     Microcomputer  24 ′, by means of LCD  16 ′, indicates that slaughtering/sanitization process is complete and instructs the operator to raise the transparent lid L 1 ′ and remove the slaughtered fish for inspection and additional processing as required.  
         [0184]     The action of raising and storing the transparent lid L 1 ′ causes microcomputer  24 ′, by means of LCD  16 ′, to instruct the operator to initiate the decontamination process, as follows.  
       Fish Slaughtering/Sanitization Tank Empty  
       [0185]     Tank  26 ′ should be emptied following completion of a fish slaughtering/sanitization process or when the PZT detector X 1 ′ detects the presence of water transient cavitation, whereupon microcomputer  24 ′ opens relay contact K 7 ′, thereby ceasing the propagation of ultrasonic irradiation, and by means of LCD  16 ′ instructs the operator to remove all remaining fish then empty tank  26 ′.  
         [0186]     PZT detector X 1 ′ feeds a 10 kHz acceptor circuit A 4  and a 10 kHz rejector circuit R 2 ′ which feed an adjustable gain narrow-band sub or harmonic amplifier A 1 ′ and the adjustable-gain broadband amplifier A 2 ′ whose outputs are fed to microcomputer  24 ′. Acceptor circuit A 4 ′ and rejector circuit R 2 ′ may employ harmonics or sub-harmonics. From amplifiers A 1 ′ and A 2 ′ outputs microcomputer  24 ′ determines the presence or absence of transient cavitation.  
         [0187]     To initiate automatic tank  26 ′ emptying the operator uses the sequencer touchpad  22 ′ to scroll LCD  16 ′ up or down until the headline “Fish Slaughtering/Sanitization Tank Empty” is flashing in the LCD display  16 ′.  
         [0188]     To commence automatic emptying or tank  26 ′ the operator depresses the start touchpad  18 ′, causing microcomputer  24 ′ to initiate the following actions.  
         [0189]     Microcomputer  24 ′ opens relay contact K 3 ′ de-energising solenoid S 3 ′, thereby opening the drain in readiness for emptying tank  26 ′. After a preset time, sufficient for tank  26 ′ to be emptied, microcomputer  24 ′, by means of LCD  16 ′, requires the operator to proceed to the decontamination process.  
       Tank Decontamination  
       [0190]     Tank decontamination consists of three automatic procedures: 1) filling tank  26 ′ with water and a preset volume of disinfectant, 2) ultrasonically agitating the water and disinfectant mixture for a preset time period, and 3) emptying tank  26 ′.  
         [0191]     During the period of decontamination, audible and visual annunciators including a “flashing” LCD  16 ′ display are active signifying an “Operator Precautionary” condition is in progress.  
         [0192]     To initiate automatic decontamination of tank  26 ′, the operator selectively depresses the sequencer touchpad  22 ′ to scroll LCD  16 ′ up or down until the headline “Tank Automatic Decontamination” is flashing in the LCD display  16 ′.  
         [0193]     To commence automatic filling of tank  26 ′, the operator depresses the start touchpad  18 ′, causing microcomputer  24 ′ to initiate the following actions.  
         [0194]     Relay contact K 3 ′ is closed, causing solenoid S 3 ′ to activate and close the drain. Microcomputer  24 ′ then closes relay contacts K 5 ′ and K 6 ′ which activate solenoids S 1 ′ and S 2 ′. Solenoid S 2 ′ causes the municipal water to flow through backflow preventer BP 2 ′ through Venturi injector I 1 ′ and 90-degree elbow  34 ′ and faucet  35 ′. Concomitantly, as the municipal water passes through injector I 1 ′, the drop in water pressure created on the exit side of injector I 1 ′ sucks out disinfectant  38 ′ from its container in a metered flow which, in combination with the municipal water flow, creates the required dilution for the required germicidal solution.  
         [0195]     When the water level in tank  26 ′ reaches level sensor D 2 ′, microprocessor  24 ′ opens relay contacts K 5 ′ and K 6 ′, closing off the supply of municipal water and disinfectant  38 ′. To commence ultrasonic agitation microcomputer  24 ′ closes relay contact K 1 ′ causing the stepper motor M 1 ′ to rotate autotransformer T 2 ′ until its encoder E 1 ′ matches the decontamination code stored in the memory of microcomputer  24  whereafter microcomputer  24 ′ closes relay K 7 ′ which causes oscillator O 1 ′ and Amplifier A 3 ′ to drive ultrasonic transducer T 1 ′ for the programmed decontamination time period.  
         [0196]     To bring the decontamination process to an end microcomputer  24 ′ opens relay contacts K 1 ′ and K 7 ′, shutting down ultrasonic transmission from transducer T 1 ′ then opens relay contact K 3 ′ which deactivates solenoid S 3 ′ opening the drain and emptying tank  26 ′. Microcomputer  24 ′ by means of LCD  16 ′ instructs the operator to depress the start touchpad  18 ′ then manually rinse and dry tank  26 ′. After completion of rinsing and drying of tank  26 ′, microcomputer  24 ′, by means of LCD  16 ′, requests the operator to depress the abort touchpad  20 ′ whereupon microcomputer  24 ′ places its request for manual rinsing and drying into its long-term memory.  
         [0197]     After a preset time period microcomputer  24 ′ shuts down all electric power ending the automatic decontamination process.  
         [0198]     Tank  26 ′ may be incorporated into the wound treatment apparatus of  FIGS. 1-6  as tank  48  ( FIG. 3 ). The function of transducers T 1 ′ ( FIG. 9 ) may be performed by transducers  66  provided in bottom surface  68  of tank  48 . Injector  11 ′, elbow  34 ′ and faucet  36 ′ are provided at an upper end of tank  48 . Two fixed side panels  25 ′ and  27 ′ ( FIG. 9 ) of tank  26 ′ (in the stand alone version of the fish killing and tissue sanitization apparatus) are replaced by louvered barriers  50  and  52  which limit fish movement between tanks  40 ,  42  and the ultrasonic tank  48 , as discussed hereinabove with reference to the wound healing apparatus of  FIGS. 1-6 . Because of residual disinfectant leakage potential between tanks  40 ,  42  and  48 , the tank decontamination process should not include disinfectant. Microcomputer  24  is programmed to include the slaughtering and sanitization functionality discussed above with reference to microcomputer  24 ′ as well as the wound healing functionality discussed above with reference to  FIG. 1 .  
         [0199]     Although the invention has been described in terms of particular embodiments and applications, one of ordinary skill in the art, in light of this teaching, can generate additional embodiments and modifications without departing from the spirit of or exceeding the scope of the claimed invention. Accordingly, it is to be understood that the drawings and descriptions herein are proffered by way of example to facilitate comprehension of the invention and should not be construed to limit the scope thereof.