Abstract:
Specific DNA sequences for binding various foodborne and waterborne pathogens and biotoxins are described. Each of these sequences can function in varying assay and sensor formats with varying degrees of success.

Description:
PRIORITY INFORMATION 
       [0001]    This application is based upon and claims priority from U.S. Provisional application Ser. No. 61/372,649 filed on Aug. 11, 2010, which is incorporated herein by reference. 
     
    
     BACKGROUND OF THE INVENTION 
       [0002]    1. Field of the Invention 
         [0003]    The present invention pertains to the field of aptamer- and nucleic acid ligand (DNA and RNA ligand)-based diagnostics. More particularly, it relates to single-stranded Deoxyribonucleic acid (“DNA”) and Ribonucleic acid (“RNA”) ligand sequences, whether individual or linked together to form longer multiple binding site “receptors,” that specifically target and bind to foodborne and waterborne pathogenic bacteria or parasites such as  Campylobacter jejuni , pathogenic  Escherichia coli, Listeria monocytogenes, Salmonella enterica  serovar  Typhimurium  (formerly  S. typhimurium ), molds or other pathogenic fungi,  Cryptosporidium  and  Giardia  parasites and related toxins produced by some bacteria (e.g., Shiga or Vero toxins) and other virulence factors (intimins, adhesions, capsules, etc.) indicating the presence of the pathogens. 
         [0004]    These individual or linked DNA ligand (aptamer) sequences represent valuable target analyte-responsive components of diagnostic devices or biosensors. A biosensor can be defined as any device that employs a biologically-derived molecule as the sensing component and transduces a target analyte binding event into a detectable physical signal (including, but not limited to, changes in light intensity, absorbance, emission, wavelength, color, electrical conduction, electrical resistance, or other electrical properties, etc). Once bonded with the target, these DNA ligand sequences can be used to qualitatively determine the presence of target analyte, as well as to quantify the target analyte amount, in a sample using a broad variety of assay types and diagnostic or sensor platforms including, but not limited to, affinity-based lateral flow test strips, membrane blotting, surface plasmon resonance (“SPR”), magnetic bead (“MB”)-based capture, plastic-adherent sandwich assays (“PASA”), chemiluminescence (“CL”), electrochemiluminescence (“ECL”), radioisotopic, fluorescence intensity, including quantum dot (“QD”) or other fluorescent nanoparticle (“FNP”) of dye-based, fluorescence lifetime, and fluorescence polarization (“FP”) assays or enzyme-linked (ELISA-like) microplate assays. ELISA-like assays refer to microwell or microplate assays similar to traditional “Enzyme-Linked Immunosorbent Assays” or “ELISA” in which an aptamer or nucleic acid ligand is substituted for the antibody component or components, but the other components such as peroxidase or alkaline phosphatase enzymes and color-producing substrates remain the same. 
         [0005]    In addition, these DNA ligand sequences are valuable in competitive displacement assays which are not solely dependent on high affinity (strong attractive forces between a receptor and its ligand) or high avidity (high tensile or physical strength of receptor-ligand bonds) to produce sensitive detection (sub-nanoMolar or sub-nanogram levels), because the equilibrium constant (generally K a =10 6  to 10 8  to enable competition) must allow reasonable displacement of previously bound target materials to detect a change at or below nanogram or nanoMolar levels. In a competitive displacement assay, labeled DNA ligand plus labeled analyte complexes compete with unlabeled analyte to bind with the labeled DNA. After allowing the labeled and unlabeled analytes to come to equilibrium with the labeled DNA, the unlabeled target analyte may be quantitatively assayed by fluorescence intensity or other methods. Such assays would include competitive displacement fluorescence resonance energy transfer (“FRET”) assays or DNA ligand “beacon” FRET assays. Each of these types of assays and detection platforms has different applications in either central laboratories or as portable detectors to identify tainted foods and water either in the field (e.g. on farms or in water supplies) or in the food processing chain progressing toward the human or animal consumer. 
         [0006]    2. Background Information 
         [0007]    The DNA ligand sequences listed in Table 1 herein were derived by iterative cycles of affinity-based selection, washing, heated elution, and polymerase chain reaction (“PCR”) amplification of bound DNA ligands from a randomized library using immobilized target analytes for affinity selection and PCR amplification followed by cloning and Sanger dideoxynucleotide DNA sequencing. Sanger dideoxynucleotide sequencing refers to DNA chain termination due to lack of a 3′-OH to link incoming bases with during DNA synthesis followed by automated fluorescence reading of the DNA sequence from an electrophoresis gel containing all of the terminated DNA fragments. DNA sequencing may be accomplished by PCR doped with dideoxynucleotides lacking hydroxyl groups at the 2′ and 3′ sugar ring positions and thereby disallowing chain formation. PCR refers to the enzymatic amplification or copying of DNA molecules with a thermo-stable DNA polymerase such as  Thermus aquaticus  polymerase (“Taq”) with known “primer” regions or short oligonucleotides of known sequence that can hybridize to a longer target DNA sequence to enable priming of the chain reaction (exponential doubling of the DNA target copy number with each round of amplification). A randomized library can be chemically synthesized by linking together the four deoxynucleotide triphosphate bases (adenine; A, cytosine; C, guanine; G, and thymine; T) in equal amounts (25% each), so that a combinatorial oligonucleotide arises with sequence diversity equal to 4 raised to the nth power (4 n ) where n is the desired length of the randomized region in bases. In other words, if position 1 in an oligonucleotide is allowed to consist of A, C, G, or T (diversity=4) by equal availability of all 4 bases and these 4 possibilities are multiplied by each base linking to 4 more possible bases at position 2, then this process yields 16 possible 2-base oligonucleotides (i.e., AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT) and so on for the entire chosen length (n) of the randomized region. This combinatorial progression displays immense diversity as a function of oligonucleotide chain length. For example, an oligonucleotide decamer of 10 base length could be expected to contain 4 n =4 10  or 1,048,576 unique DNA sequences from which to chose or select by affinity one or more sequences that bind a given immobilized target analyte. The randomized oligonucleotide or DNA is designed to be flanked on either side by short primer regions of known and fixed sequences to enable PCR amplification (exponential copying) of the rare sequences that are selected from the random library by binding to the target after the non-binding members of the random library are washed away (not selected). 
         [0008]    Additional assays, such as ELISA-like plate assays or fluorescence (intensity and FRET) assays, may be used to screen or verify the value of particular DNA and RNA ligands or aptamer sequences for detection of a given target analyte in a given assay format or type of biosensor. Some of the sequences operate (bind and transduce the binding signal) more effectively in affinity-based (ELISA-like or fluorescence intensity) assays, while other DNA ligand sequences against the same targets function better in competitive or other assays, thereby leading to more sensitive detection with lower limits of detection (sub-nanoMolar or sub-nanogram) and less cross-reactivity or more specificity for the target analyte. Specificity means the ability to selectively exclude molecules similar in structure to the true target analyte that may interfere with the assay and give false readings. All of the listed DNA ligand nucleotide sequences have potential applications in some type of assay format, because they have survived at least 5 rounds of affinity-based selection and enrichment (by PCR amplification), although some of the sequences will undoubtedly perform better in certain assay formats or configurations (in tubes, square cuvettes, membranes, or on biochips) than others. 
         [0009]    Combinations of the DNA ligands whether in whole or in part (i.e., their binding sites of 5-10 or more nucleotides or bases) could be linked together in a linear or 2-dimensional or 3-dimensional fashion similar to dendrimers to bind multiple epitopes or binding sites on a complex target analyte (Ag or antigen). The advantage of linking aptamers or their shorter binding pockets, loops or binding sites is that the nascent linear, 2-D or 3-D aptamer construct will likely have improved affinity or “avidity” (tensile binding strength) making it more difficult to remove or dissociate from the target antigen. The linked aptamer complex will be likely to gain specificity as well since the probability of binding to multiple epitopes with any degree of success is multiplicative. Thus, the ability to bind to epitopes A, B and C equals the product of the probability of binding to A with high affinity times the probability of binding to B with high affinity times the probability of binding to C with high affinity and that probability is clearly much less than binding to only A, B, or C or any combination of the two epitopes therein. In this way, the specificity of aptamers or DNA ligands can be increased. This approach to binding site linkage emulates that of nature in that antibodies demonstrate linkage of their “hypervariable” (HV) regions on the antigen combining sites of the immunoglobulin light and heavy chains. In the HV regions, the variability of the 20 amino acid types is quite high and essentially represents a selection of one combination from a large combinatorial library in the protein realm. The trait of HV region linkage contributes to antibody affinity, avidity and specificity. Similarly, linking aptamers or aptamer binding sites for various epitopes in one, two or three dimensions will enhance larger aptamer or DNA ligand construct affinity, avidity, and selectivity or specificity. 
         [0010]    All of the listed DNA ligand nucleotide sequences have potential utility in some assay format, although some of the candidate sequences will perform better in certain assay formats or configurations (in tubes, cuvettes, membranes, or on biochips) than others. Assays such as ELISA-like plate assays or fluorescence (intensity and FRET) assays, may be used to verify the utility of the DNA ligand sequences. Some of the sequences function more effectively in affinity-based (ELISA-like or fluorescence intensity) assays, while other DNA ligand sequences against the same bacterial targets or analytes function better in competitive FRET assays. 
       SUMMARY OF THE INVENTION 
       [0011]    The present invention provides specific DNA sequence information as shown in Table 1 for nucleic acid ligands selected from randomized pools to bind targeted foodborne and waterborne pathogenic bacteria and toxins, which can be put into a composition useful in a variety of assay formats and sensor or diagnostic platforms to detect or quantify the targeted bacteria or toxin. While all of the candidate sequences have been shown to bind their cognate targets, some are shown to function more effectively in affinity-based assays versus fluorescence resonance energy transfer (FRET) or other assay formats that rely more on physical parameters other than affinity such as fluorophore-quencher proximity (i.e., the Förster distance). Therefore, all of the sequences are potentially valuable for detection or quantitative assays, but some may function better than others in particular assay formats. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0012]      FIG. 1  is a graph and table comparing the FRET responses of specific DNA ligands. 
           [0013]      FIG. 2  shows the secondary structures of two DNA ligands which both bind  E. coli  strain ATCC 8739, but only one loop structure in the EcO 4R DNA ligand (SEQ ID No. 88) yielded competitive FRET as illustrated by the fluorescence spectra. 
           [0014]      FIG. 3  is a graph plotting relative fluorescence intensity against the concentration of  Campylobacter jejuni  bacteria detected in chicken juice by plastic-adherent DNA sandwich assay. 
           [0015]      FIG. 4  shows graphs demonstrating detection of  Salmonella enterica  ATCC strain 13311 by plastic-adherent DNA sandwich assay. 
           [0016]      FIG. 5  shows graphs demonstrating detection of Shiga toxin type 1 by plastic-adherent DNA sandwich assay. 
       
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 
       [0017]    There is no single preferred embodiment for use of the DNA aptamer ligand sequences or linked aptamer constructs identified herein. Rather, the sequences are useful to varying extents in a variety of assay formats and sensors or diagnostic devices chosen from at least the following list: lateral flow test strips, ELISA-like or enzyme-linked microplate assays, magnetic bead-based capture assays, ECL or other chemiluminescence assays, radioisotopic assays and a variety of fluorescence assays including, but not limited to, fluorescence intensity, fluorescence lifetime, FP assays, and FRET assays (both beacon and competitive FRET in round tubes, square or flat cuvettes, or immobilized on magnetic beads, other types of microbeads, or flat surfaces such as nitrocellulose, nylon, or other membranes or on glass or plastic DNA microarrays or “biochips.” 
         [0018]    While there may appear to be considerable variability among sequences that bind the same clinical analyte targets, “epitopes” or binding sites are usually quite small (e.g., 5-10 bases) and a single target may contain numerous individual binding sites or epitopes for multiple aptamer binding. In addition, however, there is often a common or consensus sequence or common segments of 5-10 or more nucleotides in a row within otherwise different aptamer sequences that can bind a specific target epitope that may dominate the other binding sites by being more physically accessible or having stronger electrostatic, hydrogen bonding, or other attractive forces (summation of van der Waals or other weak forces). Variations in nucleotide sequences around these consensus segments or common binding sequence segments may serve to modulate the binding segment&#39;s affinity or specificity or may have no effect at all. 
       DNA Ligand (Aptamer) Selection and Generation 
       [0019]    General methods for developing DNA ligands or aptamers to the immobilized proteins, peptides, or small molecules (defined as less than 1,000 Daltons) are as follows. The protein, peptide or an amino-derivative of the small molecule (such as glucosamine in the case of D-glucose or dextrose) is then added to 2×10 9  tosyl-coated magnetic beads (MBs; e.g., Dynal brand from Invitrogen Corp. Carlsbad, Calif., 2.8 micron size) for 2 hours at 37° C. The tosyl group is a “leaving” group that allows the formation of a very stable covalent bond between primary amine groups in the target protein, peptide or amino-derivatized small molecule and therefore immobilizes the target on the surfaces of the MBs so that they can be used to probe the randomized DNA library for DNA ligands. Target molecule-conjugated MBs (or target-MBs) are collected for 2 minutes in a magnetic collection device using an external magnet and the supernate is carefully withdrawn with a pipette tip. Target-MBs are then resuspended by vortexing briefly in 1× Binding Buffer (1XBB; 0.5M NaCl, 10 mM Tris-HCl, and 1 mM MgCl 2 , pH 7.5-7.6) and washed by agitation for 5 minutes. MBs are collected and washed three times in this manner and then resuspended in 1 ml of 1XBB. 
         [0020]    MB-based DNA ligand or aptamer development is then performed using a template library sequence such as: 5′-ATCCGTCACACCTGCTCT-N 36 -TGGTGTTGGCTCCCGTAT-3′, where N 36  represents the randomized 36-base region of the DNA library (maximal sequence diversity=4 36  in theory). Primer sequences are: 5′-ATACGGGAGCCAACACCA-3′ (designated forward) and 5′-ATCCGTCACACCTGCTCT-3′ (designated reverse) to prime the template and nascent strands for PCR, respectively. The random library is reconstituted in 500 μl of sterile nuclease-free water and heated to 95° C. for 5 minutes to ensure that the DNA library is completely single-stranded and linear. The hot DNA library solution is added to 100 μl of target-MBs (2×10 8  beads) with 600 μl of sterile 2× Binding Buffer (2XBB). The DNA library and target-MB suspension (1.2 ml) is mixed at room temperature (RT, approximately 25° C.) for 1 hour. Target-MBs with any bound DNA (round 1 aptamers) are magnetically collected. The DNA-target-MB complexes are washed three times in 400 μl of sterile 1 XBB. Following the third wash, the DNA-target-MB pellet (about 75 μl) is used in a PCR reaction to amplify the bound DNA as follows. The MB pellet is split into 15 μl aliquots and added to five pre-made PCR tubes which contain most of the nonperishable ingredients of a PCR reaction beneath a wax seal. A total of 3 μl of 1:10 primer mix (10% forward primer plus 10% reverse primer) in nuclease-free deionized water or ˜20 nanomoles of each primer per ml plus 1 μl (5 U) of Taq DNA polymerase and 5 μl of 2 mM MgCl 2  are added to each of the five tubes. PCR reactions are supplemented with 0.5 μl of  E. coli  single-strand binding protein (SSBP, Stratagene Inc., La Jolla, Calif.) to inhibit high molecular weight concatamer (end to end aggregates of the DNA ligands) formation. PCR is carried out as follows: an initial 95° C. phase for 5 minutes, followed by 20 cycles of 1 minute at 95° C., 1 minute at 53° C., and 1 minute at 72° C. followed by a 72° C. completion stage for 7 minute, and refrigeration at 4° C. This constitutes the first of multiple rounds of MB-atpamer development. Iterations of the MB-aptamer development process are repeated until the desired affinity or assay sensitivity and specificity are achieved. Typically, 5-10 rounds of the MB-aptamer development process are required to achieve low ng/ml detection of target analytes. To begin the second round and all subsequent rounds, 4 complete tubes of the 5 original PCR tubes are heated to 95° C. for 5 minutes to release bound DNA from the target-MBs. The fifth tube is always retained and refrigerated as a back-up for that round of the aptamer generation process. All available DNA (25 μl per tube) is siphoned out of the hot tubes without removing the target-MBs before the tubes cool significantly and the DNA is pooled. The 100 μl of hot DNA is added to 100 μl of fresh target-MBs in 200 μl of 2XBB and allowed to mix for 1 hr at RT. Thereafter, the selection and amplification process are repeated for 3-8 more rounds with checking for 72 bp aptamer PCR products by ethidium bromide-stained 2% agarose electrophoresis after each round. Following the last round of aptamer development, aptamers are cloned into chemically competent  E. coli  and are sequenced. 
       Screening of Aptamers for Highest Affinity, Lowest Cross-Reactivity and to Determine Lower Limit of Detection by Target Titration in ELISA-like Plate Assay (“ELASA”) 
       [0021]    To evaluate, screen, and rank aptamers based on affinity against clinically relevant targets, an enzyme-linked plate assay is conducted by first immobilizing 100 μl of 1:10 diluted target (about 0.1 mg of total protein, peptide or small molecule) in 0.1M NaHCO 3  (pH 8.5) overnight at 4° C. in a covered polystyrene 96-well plate. The plate is decanted and washed three times in 250 μl of 1XBB. Each of the different 5′-biotinylated aptamers raised against the target is dissolved in 1XBB at 1.00 nmoles to 4.50 nmoles per 100 microliters and applied to their corresponding plate wells for 1 hour at room temperature (RT; ˜25° C.) with gentle mixing on an orbital shaker. The plate is decanted and washed three times in 250 μl of 1XBB for at least 5 minutes per wash with gentle mixing. One hundred μl of a 1:2,000 dilution of streptavidin-peroxidase from a 5 mg/ml stock solution in 1XBB is added per well for 30 minutes at RT with gentle mixing. The plate is decanted and washed three times with 250 μl of 1XBB per well as before. One hundred μl of ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) substrate with stabilized hydrogen peroxide is added per well for 10 minute at room temperature. Finally absorbance is quantified using a microplate reader with 405 nm optical filter. 
       Aptamer Beacons and Competitive FRET-Aptamer Assays 
       [0022]    Once key aptamers have been identified by the commonality of their sequences or their secondary stem-loop structures, the assay developer decides upon secondary structure loops (potential binding pockets) to label with an F or Q. Secondary stem-loop structures are generated by Gibbs free energy minimization with common software. At this point, one can assess aptamer “beacon” potential in FRET analyte titration experiments. The suspected short aptamer beacon loop is synthesized again with a fluorophore (F) such as TYE 665 attached to the 5′ end and quencher (Q) such as Iowa Black attached to the 3′ end (or vice versa), purified by HPLC or other form of chromatography and assessed for fluorescence output or intensity as a function of different levels of the target analyte. 
         [0023]    Alternatively, one may label the suspected binding loops internally and place an F or a Q somewhere in the mid-section of the suspected loop other than the 3′ or 5′ end (i.e., intrachain FRET). Attachment of F or Q is usually accomplished via succinimide linkage of F- or Q-succinimides added to amino-modified aptamers at specifically chosen locations in the binding pockets. Primary amine linker moieties, such as UniLink™, can be added internally at the time of chemical synthesis of aptamers. Typically 1 mg or more of an aptamer sequence is synthesized with a primary amine linker moiety located at the approximate center of each loop structure (suspected binding pockets). Each of these internally amine-labeled aptamers is then labeled with 100 μl (0.1 mg) of F-succinimide (or alternatively Q-succinimide) for 2 hours in a 37° C. incubator, followed by purification through a 1XBB-equilibrated PD-10 (Sephadex G-25; GE Healthcare) column. In the meantime, an equal molar amount of amino-modified target molecule is labeled with 0.1 mg of spectrally matched Q-succinimide (to accept photons from F) at 37° C. for 2 hours and then washed three times by centrifugation at 14,000 rpm for 10 minutes per wash and resuspension in 1 ml of 1XBB. “Spectrally matched” means that most of the wavelengths of light emitted by F can be effectively absorbed by Q because its absorbance spectrum largely overlaps the emission spectrum of F. Naturally, if the aptamer is labeled with a Q-succinimide in the alternate form of the assay, the amino-target must be labeled with an appropriately matched F-succinimide to be quenched when bound to the Q-labeled aptamer. Pooled one ml fractions of purified F-labeled DNA aptamers are mixed with an equimolar amount of Q-labeled-amino-target analyte (or vice versa in the alternate embodiment) for 30 minutes at RT with mixing in 1 XBB and then purified through an appropriate size-exclusion chromatography column (according to molecular weight of the combined F-aptamer plus Q-target complex) to produce a “FRET complex” consisting of bound F-aptamer plus Q-labeled target. 
         [0024]    Generally, the aptamer beacons or FRET-aptamer complexes are then diluted to a final concentration of 1-5 μg/ml in 1XBB and equally dispensed to polystyrene or methacrylate cuvettes in which 1 ml of unlabeled target at various concentrations in 1 XBB or diluted blood, plama, serum, saliva, aspirate or urine has been added already. Cuvettes are gently mixed for 15 to 20 minutes at RT prior to reading their fluorescence in the homogeneous beacon or competitive-displacement FRET assay formats using a spectrofluorometer having gratings to vary the excitation wavelength and emission scanning ability or handheld or otherwise portable fluorometer having a more restricted or fixed excitation and emission optical filter set with a range of wavelengths for excitation and emission. 
       Aptamer or Aptamer Binding Site Linkage in One or More Dimensions 
       [0025]    The linkage of binding sites is beneficial in terms of enhancing receptor affinity, avidity (tensile binding strength), and selectivity versus complex targets with two or more distinct epitopes. This linkage can be sequential and linear (one-dimensional as in antibody heavy and light chain linkage of HV regions) or could be expanded into two or three dimensions much like DNA dendrimers or other more complex structures known to those skilled in the art. Linear linkage by chemical synthesis is quite facile, if one knows that aptamer DNA sequences or shorter (5-10 base) binding site sequences to be linked. One long sequence can be designed to incorporate the desired aptamers or binding sites with repetitive poly-adenine, poly-cytosine, poly-guanine, poly-thymine, poly-uridine, or other intervening sequences that are unlikely to bind the target. The length of the composite aptamer construct will be limited to about 200 bases by current chemical synthesis technology. However, biosynthesis or enzymatic synthesis by PCR or asymmetric PCR (producing predominately single-stranded DNA from a template) would not be so limited and should produce aptamer constructs up to 2,000 bases before the Taq polymerase falls off the template. In this way, very lengthy 2 kilobase aptamer constructs could be made from complementary DNA templates that would enable binding of different epitopes that are distal on the surface of relatively large objects such as whole bacterial or eukaryotic cells. Again, poly-A, C, G, T, or U or other linker nucleotide segments could be designed into the cDNA template and the resultant nascent strand to ligate aptamers or aptamer binding sites together into one contiguous linear chain. 
         [0026]    For 2-D or 3-D linked aptamer structures a variety of linker chemistries are available, but the preferred embodiment is probably addition of a primary amine group somewhere in the mid-section of a larger multi-aptamer construct followed by covalent linkage of two or more such mulit-aptamer constructs by means of bifunctional linkers such as low levels (≦1%) glutaraldehyde, carbodiimides, sulfo-EGS, sulfo-SMCC or other such bifunctional linkers familiar to those skilled in conjugate chemistry. 
         [0027]    Referring to the figures,  FIG. 1  is a comparison of ELISA-like affinity-based and competitive FRET assays using the same DNA ligands. It provides a graphical comparison of the same selected family of DNA ligands that bind  E. coli  OMPs in an ELISA-like affinity-based plate assay (data table in  FIG. 1 ) with competitive FRET response spectra for the same population of DNA ligand sequences. It further is an illustration of how anti- E. coli  OMP DNA ligands are useful in an ELISA-like assay format (tabled absorbance values) and how some candidate DNA ligand sequences show greater affinity than others (i.e., have higher absorbance values approaching 1.9) for the Crook&#39;s strain (ATCC 8739) of  E. coli . The figure also demonstrates that some DNA ligand sequences with generally lower affinities (some of the shaded boxes in  FIG. 1 ) from the same population yield a greater competitive FRET response to  E. coli  8739 (i.e., are more useful in a FRET assay format, but not as useful for affinity-based assays like ELISA). The boxed values in the table of  FIG. 1  show all of the highest affinity DNA ligand sequences or wells with absorbance values greater than 1.5. The shaded values indicate wells with the greatest competitive FRET responses (wells A8, A10, B2, B8, C12, and D6). It is clear from  FIG. 1  that only two of the highest affinity DNA ligands in wells D6 and C12 also gave strong FRET responses, thereby illustrating the varied utility of different members of the same general DNA ligand family that binds  E. coli  OMPs. 
         [0028]      FIG. 2  shows the secondary structures of two DNA ligands (as determined by Vienna RNA free energy minimization software using DNA mathematical parameters at room temperature (25° C.)) shown to bind the OMPs of  E. coli  strain ATCC 8739 with moderate to high affinity by ELISA-like assay. However, only the loop or binding pocket of the DNA ligand designated EcO 4R (SEQ ID No. 88) was useful for competitive FRET as illustrated by the fluorescence spectra at the bottom of the figure when AlexaFluor 647-succinimide is used to label the putative binding pocket via a UniLink™ amine linker between bases 42 and 43 (numbered from the 5′ end) and the fluorophore-labeled EcO 4R DNA molecules is bound to Black Hole Quencher (BHQ)-3-succinimide labeled  E. coli  ATCC 8739 and competed against decreasing levels or concentrations of unlabeled  E. coli  ATCC 8739 in neat buffer. None of the other loop structures in EcO 4R or EcO 5R (SEQ ID No. 90) DNA ligands were capable of producing a FRET response in this competitive FRET format. 
         [0029]      FIG. 3  graphically illustrates an ultrasensitive detection of  Campylobacter jejuni  by a plastic-adherent sandwich assay. The graph plots relative fluorescence intensity against the concentration of the targeted,  Campylobacter jejuni , bacteria detected in chicken juice down to a level of approximately 10 bacterial cells using a one-step plastic-adherent DNA ligand-MB/DNA ligand red QD (Q-dot 655 nm) sandwich assay. Five independent readings were taken per data point with the green (Rhodamine) channel of a fluorometer. The DNA ligand sequences may be used to detect as few as 2 live or dead  C. jejuni  bacterial cells (a well-known foodborne pathogen) in neat buffer and various food matrices including diluted whole milk and poultry rinsate. 
         [0030]    In this assay, two different  C. jejuni  sequences (C2 and C3) from the SEQ ID NO&#39;s 1-6 were 5′-amine modified upon synthesis and attached to either 1,000 tosyl-M280 (2.8 micron diameter) Dynal (Invitrogen, Inc.) MB&#39;s or 0.24 picoliters of Q-dot 655 ITK reagent (Invitrogen, Inc.) per test. The C2 DNA ligand was used for capture on the surface of tosyl-MB&#39;s and the C3 DNA ligand was used as the reporter reagent after attachment to the Q-dot 655 ITK reagent via BS3 (bis-suberate bifunctional linker from Pierce Chemical Co.). The reagents were purified, mixed together and lyophilized in plastic cuvettes. The powered assays were later back-flushed with nitrogen and capped. Upon rehydration, the adherent one-step sandwich assays were used to detect live or dead  C. jejuni  cells with the very sensitive results depicted in  FIG. 2  in chicken juice. 
         [0031]      FIG. 4  demonstrates sensitive detection of the targeted  Salmonella  species by plastic-adherent sandwich assay in the range of 10 to 1,000 bacteria per mL. The graphs show the detection of  Salmonella enterica  ATCC strain 13311 in two separate trials using specific DNA ligands in a plastic-adherent DNA ligand-MB plus DNA ligand-QD dot sandwich assay format in neat buffer The particular high affinity DNA ligand sequence used for MB conjugate formation and capture was Sal 4/14R and the DNA ligand used for QD coupling and reporting was designated Sal 19F and selected from SEQ ID NO&#39;s 81-218. However, other DNA ligands from the same family give similar affinity-based results and may be useful for detection of different species or strains of  Salmonella.    
         [0032]      FIG. 5  demonstrates the ultrasensitive detection of  E. coli  Shiga toxins by plastic-adherent sandwich assay. The graphs show the detection of Shiga toxin type 1. from two separate trials using specific DNA ligands in a plastic-adherent DNA ligand-magnetic bead plus DNA ligand quantum dot sandwich assay format. Using the plastic-adherent aptamer-MB plus aptamer-QD conjugate sandwich assay described in  FIGS. 3 and 4 , similar ultrasensitive detection of Shiga or Shiga-like toxin 1 to a level of 10 ng per mL was achieved in neat buffer as shown in  FIG. 5 . The particular high affinity DNA ligand sequence used for MB conjugate formation and capture was Shiga 8/21F in one trial and 16F in the other and the DNA ligand used for QD coupling and reporting was designated Shiga 16F in both cases. All three of these DNA ligand sequences were selected from SEQ ID NO&#39;s 544-574. However, other DNA ligands from the same family give similar results and may be useful for detection of different species or strains of Shiga-like or Vero toxins. 
         [0033]    Although the invention and DNA ligand sequences have been described with reference to specific embodiments, this description is not meant to be construed in a limited sense. Various modifications of the disclosed embodiments, as well as alternative embodiments of the inventions will become apparent to persons skilled in the art upon the reference to the description of the invention. It is, therefore, contemplated that the appended claims will cover such modifications that fall within the scope of the invention. 
         [0000]    
       
         
               
             
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 DNA ligand Sequence ID Nos. 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   Campylobacter jejuni  OMPs - Fresh bacteria 
               
               
                 SEQ ID NO. 1 (C1) - CATCCGTCACACCTGCTCTGGGGAGGGTGGCGCCCGTCTCGGT 
               
               
                 GGTGTTGGCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 2 (C2) - CATCCGTCACACCTGCTCTGGGATAGGGTCTCGTGCTAGATGTG 
               
               
                 GTGTTGGCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 3 (C3) - CATCCGTCACACCTGCTCTGGACCGGCGCTTATTCCTGCTTGTG 
               
               
                 GTGTTGGCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 4 (C4) - CATCCGTCACACCTGCTCTGGAGCTGATATTGGATGGTCCGGTG 
               
               
                 GTGTTGGCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 5 (C5) - CATCCGTCACACCTGCTCTGCCCAGAGCAGGTGTGACGGATGTG 
               
               
                 GTGTTGGCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 6 (C6) - CATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTG 
               
               
                 GTGTTGGCTCCCGTATCA 
               
               
                   
               
               
                 Aged  Campylobacter jejuni  (ACj; Greater than one month at 4° C.) 
               
               
                 SEQ ID NO. 7 (ACj-1 For) - ATACGGGAGCCAACACCAGGACCAAAATAAATAATCAC 
               
               
                 AATAAAAATGCTTCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 8 (ACj-1 Rev) - ATCCGTCACACCTGCTCTAGGAAGCATTTTTATTGTGAT 
               
               
                 TATTTATTTTGGTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 9 (ACj-2 For) - ATACGGGAGCCAACACCACGCCGGGCCATAGGCGTGTG 
               
               
                 GTAGCATACTCGTACTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 10 (ACj-2 Rev) - ATCCGTCACACCTGCTCTAGTACGAGTATGCTACCACA 
               
               
                 CGCCTATGGCCCGGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 11 (ACj-3 For) - ATACGGGAGCCAACACCATAGTATAAAGACCCAATTG 
               
               
                 ACAGACTATCCTAGGCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 12 (ACj-3 Rev) - ATCCGTCACACCTGCTCTAGCCTAGGATAGTCTGTCAA 
               
               
                 TTGGGTCTTTATACTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 13 (ACj-4 For) - ATACGGGAGCCAACACCAAGAGGGGACAGAGGGTATA 
               
               
                 AGACAACTATTCTCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 14 (ACj-4 Rev) - ATCCGTCACACCTGCTCTGGGGAGAATAGTTGTCTTAT 
               
               
                 ACCCTCTGTCCCCTCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 15 (ACj-7 For) - ATACGGGAGCCAACACCAGGCGGCCGCAACTTGGTCC 
               
               
                 CCTCTTCATCCTCGGATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 16 (ACj-7 Rev) - ATCCGTCACACCTGCTCTATCCGAGGATGAAGAGGGG 
               
               
                 ACCAAGTTGCGGCCGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 17 (ACj-8 For (69)) - ATACGGGAGCCAACACCATAGTGTTGGACCAA 
               
               
                 TACGGTAACGTGTCCTTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 18 (ACj-8 Rev (69)) - ATCCGTCACACCTGCTCTCCAAGGACACGTTA 
               
               
                 CCGTATTGGTCCAACACTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 19 (ACj-9 For) - ATACGGGAGCCAACACCACGCGATACAATGTGCTAAA 
               
               
                 AAAGTTCGTGCCCCTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 20 (ACj-9 Rev) - ATCCGTCACACCTGCTCTGCAGGGGCACGAACTTTTTT 
               
               
                 AGCACATTGTATCGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 21 (ACj-10 For) - ATACGGGAGCCAACACCACGCCGAATAGTGTTCGTAT 
               
               
                 GCCACCCGCACGTGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 22 (ACj-10 Rev) - ATCCGTCACACCTGCTCTAGACACGTGCGGGTGGCAT 
               
               
                 ACGAACACTATTCGGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 23 (ACj-11 For) - ATACGGGAGCCAACACCAGGCATGACTAAAAAGGAT 
               
               
                 AACCTAATCTCTTGTTCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 24 (ACj-11 Rev) - ATCCGTCACACCTGCTCTGGAACAAGAGATTAGGTTA 
               
               
                 TCCTTTTTAGTCATGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 25 (ACj-15 For) - ATACGGGAGCCAACACCATACAGTCCACCGTATACTA 
               
               
                 GTGGTACCCAGGCGTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 26 (ACj-15 Rev) - ATCCGTCACACCTGCTCTCGACGCCTGGGTACCACTA 
               
               
                 GTATACGGTGGACTGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 27 (ACj-16 For) - ATACGGGAGCCAACACCAGGGGGCGAACAGTTACCC 
               
               
                 TTGGTCTGGACCACTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 28 (ACj-16 Rev) - ATCCGTCACACCTGCTCTGGCAGTGGTCCAGACCAAG 
               
               
                 GGTAACTGTTCGCCCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 29 (ACj-17 For) - ATACGGGAGCCAACACCAGGGGCGTCGGGCCAGGCG 
               
               
                 ACGGCCGCCGTTTCCGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 30 (ACj-17 Rev) - ATCCGTCACACCTGCTCTGCCGGAAACGGCGGCCGTC 
               
               
                 GCCTGGCCCGACGCCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 31 (ACj-18 For) - ATACGGGAGCCAACACCACGGGCCGTCCCTGGCCCG 
               
               
                 GGGGGGCGAAACGCGCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 32 (ACj-18 Rev) - ATCCGTCACACCTGCTCTCAGCGCGTTTCGCCCCCCC 
               
               
                 GGGCCAGGGACGGCCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 33 (ACj-19 For) - ATACGGGAGCCAACACCAGGCGATTACTAAGGGAAA 
               
               
                 AAAGTGTAAAACCTACCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 34 (ACj-19 Rev) - ATCCGTCACACCTGCTCTGGGTAGGTTTTACACTTTT 
               
               
                 TTCCCTTAGTAATCGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 35 (ACj-24 For) - ATACGGGAGCCAACACCACCACCCACTGGCCCGGTC 
               
               
                 CGCGGCCGCGCGCGCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 36 (ACj-24 Rev) - ATCCGTCACACCTGCTCTGGGGCGCGCGCGGCCGCG 
               
               
                 GACCGGGCCAGTGGGTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 37 (ACj-25 For) - ATACGGGAGCCAACACCAACGATATCCCTGACCAAA 
               
               
                 GACGTTAAATGCTTCCATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 38 (ACj-25 Rev) - ATCCGTCACACCTGCTCTATGGAAGCATTTAACGTCT 
               
               
                 TTGGTCAGGGATATCGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 39 (ACj-26 For) - ATACGGGAGCCAACACCAGGGCGGGGGGTTGGCGAG 
               
               
                 CAGGAATCGAGAGAGGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 40 (ACj-26 Rev) - ATCCGTCACACCTGCTCTCACCTCTCTCGATTCCTG 
               
               
                 CTCGCCAACCCCCCGCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 41 (ACj-27 For) - ATACGGGAGCCAACACCAGATGCGCTTCCTGTAATGA 
               
               
                 ACAGATCATATTTATGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 42 (ACj-27 Rev) - ATCCGTCACACCTGCTCTACATAAATATGATCTGTTC 
               
               
                 ATTACAGGAAGCGCATCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 43 (ACj-28 For) - ATACGGGAGCCAACACCAAGGTAGGTTGCCGCAGGT 
               
               
                 TGGCGACAAACCAGGTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 44 (ACj-28 Rev) - ATCCGTCACACCTGCTCTCAACCTGGTTTGTCGCCAA 
               
               
                 CCTGCGGCAACCTACCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 45 (ACj-30 For (69)) - ATACGGGAGCCAACACCATAGTGTTGGACCA 
               
               
                 ATACGGTAACGTGTCCTTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 46 (ACj-30 Rev (69)) - ATCCGTCACACCTGCTCTCCAAGGACACGTT 
               
               
                 ACCGTATTGGTCCAACACTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 47 (ACj-33 For) - ATACGGGAGCCAACACCACCCGGGTGGCGGGGTGGG 
               
               
                 TGTGGGTCGACGTTCTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 48 (ACj-33 Rev) - ATCCGTCACACCTGCTCTCCAGAACGTCGACCCACAC 
               
               
                 CCACCCCGCCACCCGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 49 (ACj-34 For) - ATACGGGAGCCAACACCAGGGGGGGGTGGCCGCAGG 
               
               
                 AAATATGCAGTCCACTATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 50 (ACj-34 Rev) - ATCCGTCACACCTGCTCTATAGTGGACTGCATATTTC 
               
               
                 CTGCGGCCACCCCCCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 51 (ACj-35 For) - ATACGGGAGCCAACACCACACACCGGGCCCGCCCCC 
               
               
                 AGCGCCCCCCTACGCACAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 52 (ACj-35 Rev) - ATCCGTCACACCTGCTCTTGTGCGTAGGGGGGCGCTG 
               
               
                 GGGGCGGGCCCGGTGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 53 (ACj-38 For) - ATACGGGAGCCAACACCATGAAGGAAACCTTGATAG 
               
               
                 CAGGAATAGTCCATTCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 54 (ACj-38 Rev) - ATCCGTCACACCTGCTCTGGGAATGGACTATTCCTGC 
               
               
                 TATCAAGGTTTCCTTCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 55 (ACj-39 For) - ATACGGGAGCCAACACCACCCGGGTGGCGGGGTGGG 
               
               
                 TGTGGGTCGACGTTCTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 56 (ACj-39 Rev) - ATCCGTCACACCTGCTCTCCAGAACGTCGACCCACA 
               
               
                 CCCACCCCGCCACCCGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 57 (ACj-40 For) - ATACGGGAGCCAACACCACGCCCGCCGGCGACTCGC 
               
               
                 TCCACTCCGTCCCGCTCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 58 (ACj-40 Rev) - ATCCGTCACACCTGCTCTGGAGCGGGACGGAGTGGA 
               
               
                 GCGAGTCGCCGGCGGGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                   Enterococcus faecalis  Teichoic Acid (TA) DNA ligands 
               
               
                 SEQ ID NO. 59 (TA5F) - CATTCACCACACCTCTGCTGGCTTGGCTAGCCTTGATGCTA 
               
               
                 AACGACCCATAGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 60 (TA5R) - GATACGGGACGACACCACACTATGGGTCGTTTAGCATCAA 
               
               
                 GGCTAGCCAAGCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                 SEQ ID NO. 61 (TA6F) - CATTCACCACACCTCTGCTGGAGGAGGAAGTGGTCTGGAG 
               
               
                 TTACTTGACATAGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 62 (TA6R) - GATACGGGACGACACCACACTATGTCAAGTAACTCCAGAC 
               
               
                 CACTTCCTCCTCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                 SEQ ID NO. 63 (TA7F) - CATTCACCACACCTCTGCTGGACGGAAACAATCCCCGGGTA 
               
               
                 CGAGAATCAGGGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 64 (TA7R) - GATACGGGACGACACCACACCCTGATTCTCGTACCCGGGGA 
               
               
                 TTGTTTCCGTCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                 SEQ ID NO. 65 (TA9F) - CATTCACCACACCTCTGCTGGAAACCTACCATTAATGAGAC 
               
               
                 ATGATGCGGTGGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 66 (TA9R) - GATACGGGACGACACCACACCACCGCATCATGTCTCATTAA 
               
               
                 TGGTAGGTTTCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                   E. coli  O157 Lipopolysaccharide (LPS) DNA ligands 
               
               
                 SEQ ID NO. 67 (E-5F) - ATCCGTCACACCTGCTCTGGTGGAATGGACTAAGCTAGCTAG 
               
               
                 CGTTTTAAAAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 68 (E-11F) - ATCCGTCACACCTGCTCTGTAAGGGGGGGGAATCGCTTTCG 
               
               
                 TCTTAAGATGACATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 69 (E-12F) - ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTG 
               
               
                 TGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 70 (E-16F) - ATCCGTCACACCTGCTCTATCCGTCACGCCTGCTCTATCCG 
               
               
                 TCACACCTGCTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 71 (E-17F) - ATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGA 
               
               
                 CGATTTGTACAAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 72 (E-18F) - ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGT 
               
               
                 CCGGAACGATAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 73 (E-19F) - ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTC 
               
               
                 CGGAACGATAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 74 (E-5R) - ATACGGGAGCCAACACCACCTTTTAAAACGCTAGCTAGCTT 
               
               
                 AGTCCATTCCACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 75 (E-11R) - ATACGGGAGCCAACACCATGTCATCTTAAGACGAAAGCGA 
               
               
                 TTCCCCCCCCTTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 76 (E-12R) - ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGG 
               
               
                 CAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 77 (E-16R) - ATACGGGAGCCAACACCAGAGCAGGTGTGACGGATAGAGC 
               
               
                 AGGCGTGACGGATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 78 (E-17R) - ATACGGGAGCCAACACCATCTTGTACAAATCGTCTTGATAT 
               
               
                 CTGCACATTTGATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 79 (E-18R) - ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATG 
               
               
                 CCTTGCCATCTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 80 (E-19R) - ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTAT 
               
               
                 GCCTTGCCATCTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                   E. coli  Outer Membrane Proteins (OMPs) - Fresh Bacteria 
               
               
                 SEQ ID NO. 81 (EcO-1F) - ATACGGGAGCCAACACCATGGTACAAGCAAACCAATAT 
               
               
                 TAGGGCCCAGACATCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 82 (EcO-1R) - ATCCGTCACACCTGCTCTCGATGTCTGGGCCCTAATATT 
               
               
                 GGTTTGCTTGTACCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 83 (EcO-2F) - ATACGGGAGCCAACACCATGATACCCTAAGGTAGGGGA 
               
               
                 GGCCTAAGCGCCACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 84 (EcO-2R) - ATCCGTCACACCTGCTCTACGTGGCGCTTAGGCCTCCCC 
               
               
                 TACCTTAGGGTATCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 85 (EcO-3F) - ATACGGGAGCCAACACCACGCATCCCCCGCCGGGCCC 
               
               
                 GCGCCCCGCTCGCAGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 86 (EcO-3R) - ATCCGTCACACCTGCTCTGTCTGCGAGCGGGGCGCGGGC 
               
               
                 CCGGCGGGGGATGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 87 (EcO-4F (73)) - ATACGGGAGCCAACACCATAATATGCCGTAAGGAG 
               
               
                 AGGCCTGTTGGGAGCGCCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 88 (EcO-4R (73)) - ATCCGTCACACCTGCTCTACGGCGCTCCCAACAGGC 
               
               
                 CTCTCCTTACGGCATATTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 89 (EcO-5F) - ATACGGGAGCCAACACCAGGAAAAAAAGAGCCTGTGAA 
               
               
                 GATTGTAATATCAGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 90 (EcO-5R) - ATCCGTCACACCTGCTCTAACTGATATTACAATCTTCAC 
               
               
                 AGGCTCTTTTTTTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 91 (EcO-7Fa) - ATCCGTCACACCTGCTCTCGGAGGTAGACTAGGATTGC 
               
               
                 GGCGGGGGGTCAGGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 92 (EcO-7Fb) - ATACGGGAGCCAACACCACAAAAGCCTTACCTAACTGC 
               
               
                 CAACAATGAATAGCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 93 (EcO-7Ra) - ATCCGTCACACCTGCTCTTGCTATTCATTGTTGGCAGTT 
               
               
                 AGGTAAGGCTTTTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 94 (EcO-7Rb) - ATACGGGAGCCAACACCATACCTGACCCCCCGCCGCAA 
               
               
                 TCCTAGTCTACCTCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 95 (EcO-8F) - ATACGGGAGCCAACACCACGACTAACACGACCGTTGGG 
               
               
                 GGGGGCTCGCGCGGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 96 (EcO-8R) - ATCCGTCACACCTGCTCTGCCCGCGCGAGCCCCCCCCAA 
               
               
                 CGGTCGTGTTAGTCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 97 (EcO-9F) - ATACGGGAGCCAACACCAGTCCCCGCCCAGCCGTGAGC 
               
               
                 CGTACCCCCGCACACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 98 (EcO-9R) - ATCCGTCACACCTGCTCTGGTGTGCGGGGGTACGGCTCA 
               
               
                 CGGCTGGGCGGGGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 99 (EcO-10F) - ATCCGTCACACCTGCTCTCAAGGTTGGGCCTGCAAGAG 
               
               
                 CAAAAACGGGGCGGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 100 (EcO-10R) - ATACGGGAGCCAACACCATCCCGCCCCGTTTTTGCTCT 
               
               
                 TGCAGGCCCAACCTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 101 (EcO-11F) - ATCCGTCACACCTGCTCTACTTGGCTTGCGACTATTAT 
               
               
                 TCACAGGGCCAAAGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 102 (EcO-11R) - ATACGGGAGCCAACACCAGTCTTTGGCCCTGTGAATA 
               
               
                 ATAGTCGCAAGCCAAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 103 (EcO-12/37/60F (69)) - ATACGGGAGCCAACACCATAGTGTTGGA 
               
               
                 CCAATACGGTAACGTGTCCTTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 104 (EcO-12/37/60R (69)) - ATCCGTCACACCTGCTCTCCAAGGACAC 
               
               
                 GTTACCGTATTGGTCCAACACTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 105 (EcO-17F) - ATCCGTCACACCTGCTCTTGGAATGTCGGTGTTTTTCC 
               
               
                 AATTCCTTGGGTCGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 106 (EcO-17R) - ATACGGGAGCCAACACCACACGACCCAAGGAATTGG 
               
               
                 AAAAACACCGACATTCCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 107 (EcO-18F) - ATCCGTCACACCTGCTCTGCGACGGCGACGCGGTCCG 
               
               
                 GGCGGGGGTGGAGGACGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 108 (EcO-18R) - ATACGGGAGCCAACACCACGTCCTCCACCCCCGCCCG 
               
               
                 GACCGCGTCGCCGTCGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 109 (EcO-19Fa) - ATACGGGAGCCAACACCAGAGGGTTCTAGGGTCACT 
               
               
                 TCCATGAGAATGGCTCACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 110 (EcO-19Fb) - ATCCGTCACACCTGCTCTGGCCTGGGGACGCGAGGG 
               
               
                 AGGCGGGGGGAGTCGTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 111 (EcO-19Ra) - ATACGGGAGCCAACACCACCACGACTCCCCCCGCCT 
               
               
                 CCCTCGCGTCCCCAGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 112 (EcO-19Rb) - ATCCGTCACACCTGCTCTGTGAGCCATTCTCATGGAA 
               
               
                 GTGACCCTAGAACCCTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 113 (EcO-20F)ATCCGTCACACCTGCTCTCACAGGGCCTCTTACTATACA 
               
               
                 GTTCTCCAGCGCTGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 114 (EcO-20R) - ATACGGGAGCCAACACCAGCAGCGCTGGAGAACTGTA 
               
               
                 TAGTAAGAGGCCCTGTG GAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 115 (EcO-21F) - ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTT 
               
               
                 TGTATCCTAAGAGAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 116 (EcO-21R) - ATACGGGAGCCAACACCATCTCTCTTAGGATACAAAG 
               
               
                 CCAAACTGAGCCCGTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 117 (EcO-22F) - ATACGGGAGCCAACACCAGGGGTGGCGAACATGGTAT 
               
               
                 AACTTGATAAGTGTGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 118 (EcO-22R) - ATCCGTCACACCTGCTCTTCACACTTATCAAGTTATAC 
               
               
                 CATGTTCGCCACCCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 119 (EcO-23F) - ATACGGGAGCCAACACCACTCCGACACCGGCCGCCGG 
               
               
                 CACCACCCACTCCCCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 120 (EcO-23R) - ATCCGTCACACCTGCTCTAGGGGGAGTGGGTGGTGCC 
               
               
                 GGCGGCCGGTGTCGGAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 121 (EcO-24F) - ATACGGGAGCCAACACCATCCGGCGCGCCCTCCTCCC 
               
               
                 CCACTGCTCCCCGCCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 122 (EcO-24R) - ATCCGTCACACCTGCTCTCGGGCGGGGAGCAGTGGGG 
               
               
                 GAGGAGGGCGCGCCGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 123 (EcO-25F) - ATACGGGAGCCAACACCATACGCAGAGGTCCCCTACC 
               
               
                 CAGGCCAGCCGGATGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 124 (EcO-25R) - ATCCGTCACACCTGCTCTGGCATCCGGCTGGCCTGGG 
               
               
                 TAGGGGACCTCTGCGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 125 EcO-26 F - ATACGGGAGCCAACACCACGAGGATTACAACTTTATGC 
               
               
                 GTGCAACCAGACACCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 126 EcO-26 R - ATCCGTCACACCTGCTCTTGGTGTCTGGTTGCACGCATA 
               
               
                 AAGTTGTAATCCTCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 127 EcO-27 F - ATACGGGAGCCAACACCATATAAACGAGGAAATAAAA 
               
               
                 CTGCAGAACACTTCCTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 128 EcO-27 R - ATCCGTCACACCTGCTCTGAGGAAGTGTTCTGCAGTTTT 
               
               
                 ATTTCCTCGTTTATATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 129 EcO-28 F(71) - ATACGGGAGCCAACACCATCACGGCAATGTCCCGA 
               
               
                 TAATGTCTTGCTTCAGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 130 EcO-28 R(71) - ATCCGTCACACCTGCTCTCGCTGAAGCAAGACATTA 
               
               
                 TCGGGACATTGCCGTGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 131 EcO-29 F - ATACGGGAGCCAACACCAAGCAATCAGTATACCCACCC 
               
               
                 GTCAAAAACATCATGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 132 EcO-29 R - ATCCGTCACACCTGCTCTGCATGATGTTTTTGACGGGTG 
               
               
                 GGTATACTGATTGCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 133 EcO-30 F - ATACGGGAGCCAACACCACGGCTTCTTGCGCCCCCCCG 
               
               
                 CGCCCGCGCCCCCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 134 EcO-30 R - ATCCGTCACACCTGCTCTGGGGGGGGCGCGGGCGCGG 
               
               
                 GGGGGCGCAAGAAGCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 135 EcO-31 F - ATACGGGAGCCAACACCAACGGAGGATGAAGAGATAA 
               
               
                 AGTAAATATCCGGGGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 136 EcO-31 R - ATCCGTCACACCTGCTCTGCCCCCGGATATTTACTTTAT 
               
               
                 CTCTTCATCCTCCGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 137 EcO-32 F - ATACGGGAGCCAACACCACCCGTGGCCTTCACCCAGCC 
               
               
                 AGGGGCCCCGTCTCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 138 EcO-32 R - ATCCGTCACACCTGCTCTCAGAGACGGGGCCCCTGGCT 
               
               
                 GGGTGAAGGCCACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 139 EcO-33 F - ATACGGGAGCCAACACCACACTACCGTCCCACCCCCTC 
               
               
                 CCAGCTCCTCCGGCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 140 EcO-33 R - ATCCGTCACACCTGCTCTCGGCCGGAGGAGCTGGGAG 
               
               
                 GGGGTGGGACGGTAGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 141 EcO-34 F - ATACGGGAGCCAACACCAATCCCCCGCCTGCGACCGAT 
               
               
                 GCACTCCCATATGTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 142 EcO-34 R - ATCCGTCACACCTGCTCTCGACATATGGGAGTGCATCG 
               
               
                 G TCGCAGGCGGGGGATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 143 EcO-35 F - ATACGGGAGCCAACACCATACATGCCCAAGGTTTCGGG 
               
               
                 TGAGGCTACCGTGAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 144 EcO-35 R - ATCCGTCACACCTGCTCTACTCACGGTAGCCTCACCCG 
               
               
                 AAACCTTGGGCATGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 145 EcO-36 F - ATACGGGAGCCAACACCATTTATGTTTCATACTTTAAAC 
               
               
                 TTGGTCGTTTGCGATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 146 EcO-36 R - ATCCGTCACACCTGCTCTATCGCAAACGACCAAGTTTA 
               
               
                 AAGTATGAAACATAAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 147 EcO-38 F - ATACGGGAGCCAACACCAGGCGTTTAATAATCGGAGCG 
               
               
                 ACAAATTCTACGCTGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 148 EcO-38 R - ATCCGTCACACCTGCTCTACAGCGTAGAATTTGTCGCT 
               
               
                 CCGATTATTAAACGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 149 EcO-40/41B F - ATACGGGAGCCAACACCACGGCAACTTCAAACCCA 
               
               
                 AGACTAAGAAAAGCTCGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 150 EcO-40/41B R - ATCCGTCACACCTGCTCTCACGAGCTTTTCTTAGTC 
               
               
                 TTGGGTTTGAAGTTGCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 151 EcO-41A F - ATACGGGAGCCAACACCATTGTAGGCGGATATTAGAC 
               
               
                 AAGACCGAATTCCATGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 152 EcO-41A R - ATCCGTCACACCTGCTCTCCATGGAATTCGGTCTTGTC 
               
               
                 TAATATCCGCCTACAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 153 EcO-42/43A F - ATACGGGAGCCAACACCAGTAGGCTAAAGTGAGG 
               
               
                 TTAATTATGTCGACAAGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 154 EcO-42/43A R - ATCCGTCACACCTGCTCTGGCCTTGTCGACATAATT 
               
               
                 AACCTCACTTTAGCCTACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 155 EcO-43B F - ATACGGGAGCCAACACCACCTCGCCCAGACGCCGGG 
               
               
                 CCCTCCCCGCCCCACCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 156 EcO-43B R - ATCCGTCACACCTGCTCTGGGGTGGGGCGGGGAGGG 
               
               
                 CCCGGCGTCTGGGCGAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 157 EcO-44 F - ATACGGGAGCCAACACCAGGTATTGGAGCTATACACGT 
               
               
                 TAACCACCGCTATTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 158 EcO-44 R - ATCCGTCACACCTGCTCTGCAATAGCGGTGGTTAACGT 
               
               
                 GTATAGCTCCAATACCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 159 EcO-45 F - ATACGGGAGCCAACACCACGCGGGGCGGGGGGGCTGG 
               
               
                 TCGCGCGGGCCTGGCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 160 EcO-45 F - ATCCGTCACACCTGCTCTCCGCCAGGCCCGCGCGACCA 
               
               
                 GCCCCCCCGCCCCGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 161 EcO-46 F - ATACGGGAGCCAACACCAAACATTGGAACAACAAACG 
               
               
                 CTAATACACGATCGCATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 162 EcO-46 R - ATCCGTCACACCTGCTCTATGCGATCGTGTATTAGCGTT 
               
               
                 TGTTGTTCCAATGTTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 163 EcO-47 F - ATACGGGAGCCAACACCAATAGATGGATAAGGGGGA A 
               
               
                 ACTGCCATTCGGTTAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 164 EcO-47 R - ATCCGTCACACCTGCTCTACTAACCGAATGGCAGTTTC 
               
               
                 CCCCTTATCCATCTATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 165 EcO-48 F - ATACGGGAGCCAACACCAACCAACGAAGAAGGGTCAG 
               
               
                 ACAAAAAGGAGTTCTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 166 EcO-48 R - ATCCGTCACACCTGCTCTCGAGAACTCCTTTTTGTCTGA 
               
               
                 CCCTTCTTCGTTGGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 167 EcO-49 F - ATACGGGAGCCAACACCACAACAGTCAGATTGCAACT 
               
               
                 GAGTAGTACATACGTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 168 EcO-49 R - ATCCGTCACACCTGCTCTTAACGTATGTACTACTCAGTT 
               
               
                 GCAATCTGACTGTTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 169 EcO-50 F - ATACGGGAGCCAACACCATAAACCAAGGGTGTAACAG 
               
               
                 AAATGATGTGACCAGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 170 EcO-50 R - ATCCGTCACACCTGCTCTGCCTGGTCACATCATTTCTGT 
               
               
                 TACACCCTTGGTTTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 171 EcO-51 F - ATACGGGAGCCAACACCATCATTGCGACATTGAATTCA 
               
               
                 GAAGGAGGAGTGGTGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 172 EcO-51 R - ATCCGTCACACCTGCTCTACACCACTCCTCCTTCTGAAT 
               
               
                 TCAATGTCGCAATGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 173 EcO-52 F (71) - ATACGGGAGCCAACACCAGAGAATTACAACAGGTT 
               
               
                 AAGTAGTGTGACGATCATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 174 EcO-52 R (71) - ATCCGTCACACCTGCTCTATGATCGTCACACTACT 
               
               
                 TAACCTGTTGTAATTCTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 175 EcO-53 F - ATACGGGAGCCAACACCACGGCGGAACACATGGAACA 
               
               
                 CCGAATAATGTGGCTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 176 EcO-53 R - ATCCGTCACACCTGCTCTTAAGCCACATTATTCGGTGTT 
               
               
                 CCATGTGTTCCGCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 177 EcO-54 F - ATACGGGAGCCAACACCAGCGTGGTGGACGTATAACAA 
               
               
                 GACAGAAGTAACCCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 178 EcO-54 R - ATCCGTCACACCTGCTCTACGGGTTACTTCTGTCTTGTT 
               
               
                 ATACGTCCACCACGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 179 EcO-55 R (71) - ATACGGGAGCCAACACCATGACAAACATCAATGCA 
               
               
                 GCAAAGACTAGCAACGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 180 EcO-55 F (71) - ATCCGTCACACCTGCTCTCACGTTGCTAGTCTTTG 
               
               
                 CTGCATTGATGTTTGTCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 181 EcO-56 F (71) - ATACGGGAGCCAACACCAGCCGGCAATGGCTGAG 
               
               
                 AGAGAATAGAGCGTGGTATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 182 EcO-56 R (71) - ATCCGTCACACCTGCTCTATACCACGCTCTATTCT 
               
               
                 CTCTCAGCCATTGCCGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 183 EcO-57 F - ATACGGGAGCCAACACCAAGGGGGCGGCGAGTCGTAG 
               
               
                 CGTCGATAATACTGGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 184 EcO-57 R - ATCCGTCACACCTGCTCTGTCCAGTATTATCGACGCTA 
               
               
                 CGACTCGCCGCCCCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 185 EcO-58 F - ATACGGGAGCCAACACCAGCCCGGAGCCCTTCGTCTGC 
               
               
                 CCGCAGTCCAGTGTATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 186 EcO-58 R - ATCCGTCACACCTGCTCTATACACTGGACTGCGGGCAG 
               
               
                 ACGAAGGGCTCCGGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 187 EcO-59 F - ATACGGGAGCCAACACCATCCGCGCCCCCGCGGCATCC 
               
               
                 GCTCACGCGTCCCGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 188 EcO-59 R - ATCCGTCACACCTGCTCTGCCGGGACGCGTGAGCGGAT 
               
               
                 GCCGCGGGGGCGCGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 189 EcO-60B F - ATACGGGAGCCAACACCATGCAGGACAAAGCGATGA 
               
               
                 GATACGATCTACCGCTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 190 EcO-60B R - ATCCGTCACACCTGCTCTCGAGCGGTAGATCGTATCT 
               
               
                 CATCGCTTTGTCCTGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 191 EcO-62 F - ATACGGGAGCCAACACCATGGAGAGGAAGACGGAAA 
               
               
                 GTATGGAGTGGATGAAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 192 EcO-62 R - ATCCGTCACACCTGCTCTCCTTCATCCACTCCATACTTT 
               
               
                 CCGTCTTCCTCTCCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 193 EcO-63 F - ATACGGGAGCCAACACCACGAAGGAGTAAAGCATGCT 
               
               
                 GTCCCTATGAGCTGGGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 194 EcO-63 R - ATCCGTCACACCTGCTCTTCCCAGCTCATAGGGACAGC 
               
               
                 ATGCTTTACTCCTTCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 195 EcO-64 F - ATACGGGAGCCAACACCACCCTGAGACATACCTAGTCA 
               
               
                 AGTGGAACAGACAGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 196 EcO-64 R - ATCCGTCACACCTGCTCTACCTGTCTGTTCCACTTGACT 
               
               
                 AGGTATGTCTCAGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 197 EcO-65 F - ATACGGGAGCCAACACCAGTAGGGAGAAGTTCGAATG 
               
               
                 AAAATACGCTACGAACAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 198 EcO-65 R - ATCCGTCACACCTGCTCTTGTTCGTAGCGTATTTTCATT 
               
               
                 CGAACTTCTCCCTACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 199 EcO-66 F - ATACGGGAGCCAACACCACTCCCTACCGGCTCTGCGGG 
               
               
                 AAATATGTTTTGACCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 200 EcO-66 R - ATCCGTCACACCTGCTCTGGGTCAAAACATATTTCCCG 
               
               
                 CAGAGCCGGTAGGGAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 201 EcO-67 F - ATACGGGAGCCAACACCACCCGTGGCCTTCACCCAGCC 
               
               
                 AGGGGCCCCGTCTCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 202 EcO-67 R - ATCCGTCACACCTGCTCTCAGAGACGGGGCCCCTGGCT 
               
               
                 GGGTGAAGGCCACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 203 EcO-68 F - ATACGGGAGCCAACACCACAAAGTCCTCCCCCCTGGGC 
               
               
                 GCCTTCACCCCACTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 204 EcO-68 R - ATCCGTCACACCTGCTCTGCGGTGGGGTGAAGGCGCCC 
               
               
                 AGGGGGGAGGACTTTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 205 EcO-69 R - ATACGGGAGCCAACACCATGGTAGACAGCGTCGCCCT 
               
               
                 GCCATCACTCCGGCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 206 EcO-69 F - ATCCGTCACACCTGCTCTGGGGCCGGAGTGATGGCAGG 
               
               
                 GCGACGCTGTCTACCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 207 EcO-70 F - ATACGGGAGCCAACACCACGATCCCGGCGCGACGGAT 
               
               
                 GTAAAATAAGTGTGCTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 208 EcO-70 R - ATCCGTCACACCTGCTCTGAGCACACTTATTTTACATCC 
               
               
                 GTCGCGCCGGGATCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 209 EcO-71 F - ATACGGGAGCCAACACCAGGGAAGATATGCAACAGAT 
               
               
                 GGTGGACCGTAGTATGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 210 EcO-71 R - ATCCGTCACACCTGCTCTCCATACTACGGTCCACCATCT 
               
               
                 GTTGCATATCTTCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 211 EcO-72 F - ATACGGGAGCCAACACCACAGCAGGGTACTGTAGTGG 
               
               
                 TGGGGGGCCGGTCCGGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 212 EcO-72 R - ATCCGTCACACCTGCTCTCCCGGACCGGCCCCCCACCA 
               
               
                 CTACAGTACCCTGCTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 213 EcO-73 F - ATACGGGAGCCAACACCAAGACGGACAGGGGACGTCG 
               
               
                 GTGAAGCGACGGATTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 214 EcO-73 R - ATCCGTCACACCTGCTCTCCAATCCGTCGCTTCACCGA 
               
               
                 CGTCCCCTGTCCGTCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 215 EcO-74 F - ATACGGGAGCCAACACCACACGAGCCAGGTAAAAGTA 
               
               
                 AGCCACACAAAGTGCTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 216 EcO-74 R - ATCCGTCACACCTGCTCTGAGCACTTTGTGTGGCTTACT 
               
               
                 TTTACCTGGCTCGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 217 EcO-75 F - ATACGGGAGCCAACACCACCACGCGACACCCCCTCCT 
               
               
                 GTCCCCCGCCCGCTTCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 218 EcO-75 R - ATCCGTCACACCTGCTCTGGAAGCGGGCGGGGGACAG 
               
               
                 GAGGGGGTGTCGCGTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 Aged  E. coli  (AEc; Greater than one month at 4° C.) 
               
               
                 SEQ ID NO. 219 AEc-47F - ATACGGGAGCCAACACCATCGAGAAGCATTGATAACAA 
               
               
                 AATTTAAACCCCTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 220 AEc-47R - ATCCGTCACACCTGCTCTGGCAGGGGTTTAAATTTTGTT 
               
               
                 ATCAATGCTTCTCGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 221 AEc-48 F - ATACGGGAGCCAACACCATCGAGAAGCATTGATAACA 
               
               
                 AAATTTAAACCCCTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 222 AEc-48R - ATCCGTCACACCTGCTCTGGCAGGGGTTTAAATTTTGTT 
               
               
                 ATCAATGCTTCTCGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 223 AEc-49 F - ATACGGGAGCCAACACCAGGATCCGTAGAATGATTTA 
               
               
                 AATAAACACGAACACATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 224 AEc-49R - ATCCGTCACACCTGCTCTATGTGTTCGTGTTTATTTAAA 
               
               
                 TCATTCTACGGATCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 LPS Core Antigen DNA ligands 
               
               
                 SEQ ID NO. 225 (Glucosamine(G)1F) - ATCCGTCACACCTGCTCTAATTAGGATACG 
               
               
                 GGGCAACAGAACGAGAGGGGGGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 226 (G2F) - ATCCGTCACACCTGCTCTCGGACCAGGTCAGACAAGCACAT 
               
               
                 CGGATATCCGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 227 (G5F) - ATCCGTCACACCTGCTCTTGAGTCAAAGAGTTTAGGGAGGA 
               
               
                 GCTAACATAACAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 228 (G7F) - ATCCGTCACACCTGCTCTAACAACAATGCATCAGCGGGCTG 
               
               
                 GGAACGCATGCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 229 (G8F) - ATCCGTCACACCTGCTCTGAACAGGTTATAAGCAGGAGTGA 
               
               
                 TAGTTTCAGGATCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 230 (G9F) - ATCCGTCACACCTGCTCTCGGCGGCTCGCAAACCGAGTGGT 
               
               
                 CAGCACCCGGGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 231 (G10F) - ATCCGTCACACCTGCTCTGCGCAAGACGTAATCCACAAGA 
               
               
                 CCGTGAAAACATAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 232 (G1R) - ATACGGGAGCCAACACCATTCCCCCCTCTCGTTCTGTTGCC 
               
               
                 CCGTATCCTAATTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 233 (G2R) - ATACGGGAGCCAACACCAGCCGGATATCCGATGTGCTTGTC 
               
               
                 TGACCTGGTCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 234 (G5R) - ATACGGGAGCCAACACCACTGTTATGTTAGCTCCTCCCTAA 
               
               
                 ACTCTTTGACTCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 235 (G7R) - ATACGGGAGCCAACACCACCGCATGCGTTCCCAGCCCGCTG 
               
               
                 ATGCATTGTTGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 236 (G8R) - ATACGGGAGCCAACACCAGATCCTGAAACTATCACTCCTG 
               
               
                 CTTATAACCTGTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 237 (G9R) - ATACGGGAGCCAACACCAACCCGGGTGCTGACCACTCGGT 
               
               
                 TTGCGAGCCGCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 238 (G10R) - ATACGGGAGCCAACACCACTATGTTTTCACGGTCTTGTGG 
               
               
                 ATTACGTCTTGCGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 239 (KDO (K) Antigen 2F) - ATCCGTCACACCTGCTCTAGGCGTAGTG 
               
               
                 ACTAAGTCGCGCGAAAATCACAGCATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 240 (K5F) - ATCCGTCACACCTGCTCTCAGCGGCAGCTATACAGTGAGAA 
               
               
                 CGGACTAGTGCGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 241 (K7F) - ATCCGTCACACCTGCTCTGGCAAATAATACTAGCGATGATG 
               
               
                 GATCTGGATAGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 242 (K8F) - ATCCGTCACACCTGCTCTGGGGGTGCGACTTAGGGTAAGTG 
               
               
                 GGAAAGACGATGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 243 (K9F) - ATCCGTCACACCTGCTCTCAAGAGGAGATGAACCAATCTTA 
               
               
                 GTCCGACAGGCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 244 (K10F) - ATCCGTCACACCTGCTCTGGCCCGGAATTGTCATGACGTC 
               
               
                 ACCTACACCTCCTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 245 (K2R) - ATACGGGAGCCAACACCAATGCTGTGATTTTCGCGCGACTT 
               
               
                 AGTCACTACGCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 246 (K5R) - ATACGGGAGCCAACACCAACGCACTAGTCCGTTCTCACTGT 
               
               
                 ATAGCTGCCGCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 247 (K7R) - ATACGGGAGCCAACACCAGTCTATCCAGATCCATCATCGC 
               
               
                 TAGTATTATTTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 248 (K8R) - ATACGGGAGCCAACACCAGCATCGTCTTTCCCACTTACCCT 
               
               
                 AAGTCGCACCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 249 (K9R) - ATACGGGAGCCAACACCACCGCCTGTCGGACTAAGATTGG 
               
               
                 TTCATCTCCTCTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 250 (K10R) - ATACGGGAGCCAACACCACAGGAGGTGTAGGTGACGTCA 
               
               
                 TGACAATTCCGGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 251 (Whole LPS from  E. coli  O111:B4 (L)1F) - ATCCGTCAC 
               
               
                 CCCTGCTCTCGTCGCTATGAAGTAACAAAGATAGGAGCAATCGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 252 (L3F) - ATCCGTCACACCTGCTCTAACGAAGACTGAAACCAAAGCAG 
               
               
                 TGACAGTGCTGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 253 (L4F) - ATCCGTCACACCTGCTCTCGGTGACAATAGCTCGATCAGCC 
               
               
                 C AAAGTCGTCAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 254 (L6F) - ATCCGTCACACCTGCTCTAACGAAATAGACCACAAATCGAT 
               
               
                 ACTTTATGTTATTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 255 (L7F) - ATCCGTCACACCTGCTCTGTCGAATGCTCTGCCTGGAAGAG 
               
               
                 TTGTTAGCAGGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 256 (L8F) - ATCCGTCACACCTGCTCTTAAGCCGAGGGGTAAATCTAGGA 
               
               
                 CAGGGGTCCATGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 256 (L9F) - ATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAA 
               
               
                 GAAGGAAGTTATGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 258 (L10F) - ATCCGTCACACCTGCTCTGGTACGAATCACAGGGGATGCT 
               
               
                 GGAAGCTTGGCTCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 259 (L1R) - ATACGGGAGCCAACACCACCCGATTGCTCCTATCTTTGTTA 
               
               
                 CTTCATAGCGACGAGAGCAGGGGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 260 (L3R) - ATACGGGAGCCAACACCATTCAGCACTGTCACTGCTTTGGT 
               
               
                 TTCAGTCTTCGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 261 (L4R) - ATACGGGAGCCAACACCATCTGACGACTTTGGGCTGATCGA 
               
               
                 GCTATTGTCACCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 262 (L6R) - ATACGGGAGCCAACACCAATAACATAAAGTATCGATTTGTG 
               
               
                 GTCTATTTCGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 263 (L7R) - ATACGGGAGCCAACACCATCCCTGCTAACAACTCTTCCAGG 
               
               
                 CAGAGCATTCGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 264 (L8R) - ATACGGGAGCCAACACCATCATGGACCCCTGTCCTAGATTT 
               
               
                 ACCCCTCGGCTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 265 (L9R) - ATACGGGAGCCAACACCACATAACTTCCTTCTTAGTCATGC 
               
               
                 TGAGCCGGCCAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 266 (L10R) - ATACGGGAGCCAACACCAAGAGCCAAGCTTCCAGCATCCC 
               
               
                 CTGTGATTCGTACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 267 (Rough (Ra or R) Core LPS Antigens R1F) - ATCCGTC 
               
               
                 ACACCTGCTCTCCGCACGTAGGACCACTTTGGTACACGCTCCCGTAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 268 (R5F) - ATCCGTCACACCTGCTCTACGGATGAACGAAGATTTTAAAG 
               
               
                 TCAAGCTAATGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 269 (R6F) - ATCCGTCACACCTGCTCTGTAGTGAAGAGTCCGCAGTCCAC 
               
               
                 GCTGTTCAACTCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 270 (R7F) - ATCCGTCACACCTGCTCTACCGGCTGGCACGGTTATGTGTGA 
               
               
                 CGGGCGAAGATATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 271 (R9F) - ATCCGTCACACCTGCTCTGCGTGTGGAGCGCCTAGGTGAGT 
               
               
                 GGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 272 (R10F) - ATCCGTCACACCTGCTCTGATGTCCCTTTGAAGAGTTCCAT 
               
               
                 GACGCTGGCTCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 273 (R1R) - ATACGGGAGCCAACACCACTACGGGAGCGTGTACCAAAGT 
               
               
                 GGTCCTACGTGCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 274 (R5R) - ATACGGGAGCCAACACCATGCATTAGCTTGACTTTAAAATC 
               
               
                 TTCGTTCATCCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 275 (R6R) - ATACGGGAGCCAACACCATGAGTTGAACAGCGTGGACTGC 
               
               
                 GGACTCTTCACTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 276 (R7R) - ATACGGGAGCCAACACCATATCTTCGCCCGTCACACATAAC 
               
               
                 CGTGCCAGCCGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 277 (R9R) - ATACGGGAGCCAACACCACTCACCTAGGCGCTCCACACGC 
               
               
                 AGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 278 (R10R) - ATACGGGAGCCAACACCAAGGAGCCAGCGTCATGGAACTC 
               
               
                 TTCAAAGGGACATCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 Listeriolysin (A surface protein on  Listeria monocytogenes ) DNA  
               
               
                 ligands 
               
               
                 SEQ ID NO. 279 (LO-10F) - ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGC 
               
               
                 TGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 280 (LO-11F) - ATCCGTCACACCTGCTCTGGTGGAATGGACTAAGCTAGC 
               
               
                 TAGCGTTTTAAAAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 281 (LO-13F) - ATCCGTCACACCTGCTCTTAAAGTAGAGGCTGTTCTCCA 
               
               
                 GACGTCGCAGGAGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 282 (LO-15F) - ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCG 
               
               
                 TCCGGAACGATAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 283 (LO-16F) - ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCG 
               
               
                 TCCGGAACGATAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 284 (LO-17F) - ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCC 
               
               
                 GGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 285 (LO-19F) - ATCCGTCACACCTGCTCTTGGGCAGGAGCGAGAGACTCT 
               
               
                 AATGGTAAGCAAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 286 (LO-20F) - ATCCGTCACACCTGCTCTCCAACAAGGCGACCGACCGCA 
               
               
                 TGCAGATAGCCAGGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 287 (LO-10R) - ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCC 
               
               
                 GGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 288 (LO-11R) - ATACGGGAGCCAACACCACCTTTTAAAACGCTAGCTAG 
               
               
                 CTTAGTCCATTCCACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 289 (LO-13R) - ATACGGGAGCCAACACCATCCTCCTGCGACGTCTGGAGA 
               
               
                 ACAGCCTCTACTTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 290 (LO-15R) - ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTA 
               
               
                 TGCCTTGCCATCTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 291 (LO-16R) - ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTA 
               
               
                 TGCCTTGCCATCTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 292 (LO-17R) - ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGC 
               
               
                 TGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 293 (LO-19R) - ATACGGGAGCCAACACCATTCTTGCTTACCATTAGAGTC 
               
               
                 TCTCGCTCCTGCCCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 294 (LO-20R) - ATACGGGAGCCAACACCAACCTGGCTATCTGCATGCGGT 
               
               
                 CGGTCGCCTTGTTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 Listeriolysin (Alternate form of  Listeria  surface protein  
               
               
                 designated “Pest-Free”) DNA ligands 
               
               
                 SEQ ID NO. 295 (LP-3F) - ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGT 
               
               
                 CCGGAACGATAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 296 (LP-11F) - ATCCGTCACACCTGCTCTAACCAAAAGGGTAGGAGACCA 
               
               
                 AGCTAGCGATTTGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 297 (LP-13F) - ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCT 
               
               
                 GTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 298 (LP-14F) - ATCCGTCACACCTGCTCTGAAGCCTAACGGAGAAGATGG 
               
               
                 CCCTACTGCCGTAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 299 (LP-15F) - ATCCGTCACACCTGCTCTACTAAACAAGGGCAAACTGTA 
               
               
                 AACACAGTAGGGGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 300 (LP-17F) - ATCCGTCACACCTGCTCTGGTGTTGGCTCCCGTATAGCTT 
               
               
                 GGCTCCCGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 301 (LP-18F) - TCCGTCACACCTGCTCTGTCGCGATGATGAGCAGCAGCG 
               
               
                 CAGGAGGGAGGGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 302 (LP-20F) - ATCCGTCACACCTGCTCTGATCAGGGAAGACGCCAACAC 
               
               
                 TGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 303 (LP-3R) - ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTA 
               
               
                 TGCCTTGCCATCTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 304 (LP-11R) - ATACGGGAGCCAACACCATCCAAATCGCTAGCTTGGTC 
               
               
                 TCCTACCCTTTTGGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 305 (LP-13R) - ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCG 
               
               
                 GCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 306 (LP-14R) - ATACGGGAGCCAACACCACCTACGGCAGTAGGGCCATC 
               
               
                 TTCTCCGTTAGGCTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 307 (LP-15R) - ATACGGGAGCCAACACCACGCCCCTACTGTGTTTACAG 
               
               
                 TTTGCCCTTGTTTAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 308 (LP-17R) - ATACGGGAGCCAACACCATACGGGAGCCAAGCTATACG 
               
               
                 GGAGCCAACACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 309 (LP-18R) - ATACGGGAGCCAACACCACCCCCTCCCTCCTGCGCTGCT 
               
               
                 GCTCATCATCGCGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 310 (LP-20R) - ATACGGGAGCCAACACCAGTGTTGGCGTCTTCCCTGATC 
               
               
                 AGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                   Listeria monocytogenes  Whole Cell (LmW) 
               
               
                 SEQ ID NO. 311 LmW-2 F - ATACGGGAGCCAACACCAATACCTGTAAAAGTCTGAG 
               
               
                 AAGTGGAGTAACCTAGAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 312 LmW-2 R - ATCCGTCACACCTGCTCTCTAGGTTACTCCACTTCTCA 
               
               
                 GACTTTTACAGGTATTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 313 LmW-3 F - ATACGGGAGCCAACACCACCGACCAACAGTAATAGCC 
               
               
                 TAAAAGAGTTATGCGCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 314 LmW-3 R - ATCCGTCACACCTGCTCTAGCGCATAACTCTTTTAGGCT 
               
               
                 ATTACTGTTGGTCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 315 LmW-5 F - ATACGGGAGCCAACACCAGGTGGACTATATATGAAGTT 
               
               
                 AGTGAGCTTTAACAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 316 LmW-5 R - ATCCGTCACACCTGCTCTCCTGTTAAAGCTCACTAACT 
               
               
                 TCATATATAGTCCACCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 317 LmW-8 F - ATACGGGAGCCAACACCACAGGAGAGGCAGTAAAAG 
               
               
                 GGTTGGCTGCCTGGGTAGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 318 LmW-8 R - ATCCGTCACACCTGCTCTCTACCCAGGCAGCCAACCCT 
               
               
                 TTTACTGCCTCTCCTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 319 LmW-9 F - ATACGGGAGCCAACACCATTAGCAAGGTAAGAACAGT 
               
               
                 TTTAATACATGCCTTCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 320 LmW-9 R - ATCCGTCACACCTGCTCTGGAAGGCATGTATTAAAACT 
               
               
                 GTTCTTACCTTGCTAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 321 LmW-10 F - ATACGGGAGCCAACACCATAACAAATAACCACCCTC 
               
               
                 AATGCTAGATAGTGGCTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 322 LmW-10 R - ATCCGTCACACCTGCTCTAAGCCACTATCTAGCATTGA 
               
               
                 GGGTGGTTATTTGTTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 323 LmW-11 F - ATACGGGAGCCAACACCAGGAACATGATAAGTGAGA 
               
               
                 AGTGCGACGTTAGCTTATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 324 LmW-11 R - ATCCGTCACACCTGCTCTATAAGCTAACGTCGCACTTC 
               
               
                 TCACTTATCATGTTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 325 LmW-12 F - ATACGGGAGCCAACACCAAAAGGGTGTTCATACGGA 
               
               
                 ATGTAGATCGCCTAAGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 326 LmW-12 R - ATCCGTCACACCTGCTCTCACTTAGGCGATCTACATTC 
               
               
                 CGTATGAACACCCTTTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 327 LmW-13 F - ATACGGGAGCCAACACCATGAGCACCGGCAAACGCG 
               
               
                 TAGGTTAAGCTACATGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 328 LmW-13 R - ATCCGTCACACCTGCTCTGTCATGTAGCTTAACCTACG 
               
               
                 CGTTTGCCGGTGCTCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 329 LmW-14 F - ATACGGGAGCCAACACCATGATTAGATACTGCCTAGC 
               
               
                 TGTGTGCTCGTTGGGGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 330 LmW-14 R - ATCCGTCACACCTGCTCTCCCCCAACGAGCACACAGC 
               
               
                 TAGGCAGTATCTAATCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 331 LmW-19 F - ATACGGGAGCCAACACCAGGAGGAAGGTCAGCGTTC 
               
               
                 TTCACGTGGCTAGGGGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 332 LmW-19 R - ATCCGTCACACCTGCTCTGCCCCCTAGCCACGTGAAG 
               
               
                 AACGCTGACCTTCCTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 333 LmW-22 F - ATACGGGAGCCAACACCAACTGAGCTATATCTAGATC 
               
               
                 GACTTACACATACACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 334 LmW-22 R - ATCCGTCACACCTGCTCTACGTGTATGTGTAAGTCGAT 
               
               
                 CTAGATATAGCTCAGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 335 LmW-23 F - ATACGGGAGCCAACACCAGAGACGTGTGAAGTCCAG 
               
               
                 G CAGGGTGCCTTCTGTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 336 LmW-23 R - ATCCGTCACACCTGCTCTCGACAGAAGGCACCCTGCC 
               
               
                 TGGACTTCACACGTCTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 337 LmW-24 F - ATACGGGAGCCAACACCATCAAGTGGTGAGCGCCTCG 
               
               
                 TCGGGAACTGCCGTGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 338 LmW-24 R - ATCCGTCACACCTGCTCTCGCACGGCAGTTCCCGACG 
               
               
                 AGGCGCTCACCACTTGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 339 LmW-25 F - ATACGGGAGCCAACACCACCGCTGAAACCTCTCCGCC 
               
               
                 GTCCCGCCCTCCTCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 340 LmW-25 R - ATCCGTCACACCTGCTCTGGGGAGGAGGGCGGGACGG 
               
               
                 CGGAGAGGTTTCAGCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 341 LmW-27 F - ATACGGGAGCCAACACCAGGAGATGGTAGCACTAAA 
               
               
                 ATACGACGTATGCTGTGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 342 LmW-27 R - ATCCGTCACACCTGCTCTACACAGCATACGTCGTATTT 
               
               
                 TAGTGCTACCATCTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 343 LmW-28 F - ATACGGGAGCCAACACCAAATCGACCGGACTAATCCT 
               
               
                 GTGACTCCCCTATGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 344 LmW-28 R - ATCCGTCACACCTGCTCTAGACATAGGGGAGTCACAG 
               
               
                 G ATTAGTCCGGTCGATTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 345 LmW-30 F - ATACGGGAGCCAACACCAAATTCAATTGCGCACGTAA 
               
               
                 GAATAGATAGGCTGACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 346 LmW-30 R - ATCCGTCACACCTGCTCTGGTCAGCCTATCTATTCTTA 
               
               
                 CGTGCGCAATTGAATTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 347 LmW-31 F - ATACGGGAGCCAACACCACACACAGAGCGCCATGGA 
               
               
                 CTCAGTCAGATGTGATGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 348 LmW-31 R - ATCCGTCACACCTGCTCTACATCACATCTGACTGAGT 
               
               
                 CCATGGCGCTCTGTGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 349 LmW-32 F - ATACGGGAGCCAACACCATCCAATGAGGCCATGGACC 
               
               
                 GGTAAACTCGGACGCGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 350 LmW-32 R - ATCCGTCACACCTGCTCTGCGCGTCCGAGTTTACCGGT 
               
               
                 CCATGGCCTCATTGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 351 LmW-33 F - ATACGGGAGCCAACACCAACCCCGGTACCATCCGAC 
               
               
                 ACCACGAGCACCCGGACGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 352 LmW-33 R - ATCCGTCACACCTGCTCTCGTCCGGGTGCTCGTGGTGT 
               
               
                 CGGATGGTACCGGGGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 353 LmW-34 F - ATACGGGAGCCAACACCAAGGCGAAACTATTCACAG 
               
               
                 AGACTGATCCAGCAAGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 354 LmW-34 R - ATCCGTCACACCTGCTCTACCTTGCTGGATCAGTCTCT 
               
               
                 GTGAATAGTTTCGCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 355 LmW-35 F - ATACGGGAGCCAACACCAAACACATAGTCGTGGCAG 
               
               
                 AACGAATACTTAGCGCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 356 LmW-35 R - ATCCGTCACACCTGCTCTCCGCGCTAAGTATTCGTTCT 
               
               
                 GCCACGACTATGTGTTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 357 LmW-36 F - ATACGGGAGCCAACACCAACACGATCGACGGCGCTT 
               
               
                 GGTCCCTTACAACCCTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 358 LmW-36 R - ATCCGTCACACCTGCTCTGCAGGGTTGTAAGGGACCA 
               
               
                 AGCGCCGTCGATCGTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 359 LmW-37 F - ATACGGGAGCCAACACCAAACCAGGACTCTGTCGCTC 
               
               
                 TAAACATGACCATCGATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 360 LmW-37 R - ATCCGTCACACCTGCTCTATCGATGGTCATGTTTAGA 
               
               
                 GCGACAGAGTCCTGGTTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 361 LmW-39 F - ATACGGGAGCCAACACCACAACCACTGTAGGCTCAT 
               
               
                 GTAACTACCCGTTGTTGAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 362 LmW-39 R - ATCCGTCACACCTGCTCTCAACAACGGGTAGTTACAT 
               
               
                 GAGCCTACAGTGGTTGTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 363 LmW-40 F - ATACGGGAGCCAACACCAGGGGACAAGCAGAACCGA 
               
               
                 ACAGATTGCAACGTATCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 364 LmW-40 R - ATCCGTCACACCTGCTCTGGATACGTTGCAATCTGTTC 
               
               
                 GGTTCTGCTTGTCCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 365 LmW-41 F - ATACGGGAGCCAACACCAGCGCTTGAACAACATAAT 
               
               
                 GCCGCCCAAGACCTTGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 366 LmW-41 R - ATCCGTCACACCTGCTCTGTCAAGGTCTTGGGCGGCA 
               
               
                 TTATGTTGTTCAAGCGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 367 LmW-42 F - ATACGGGAGCCAACACCACAGTGCCTAGACTTTTACA 
               
               
                 ATGAACCAATTGCTGGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 368 LmW-42 R - ATCCGTCACACCTGCTCTTCCAGCAATTGGTTCATTGT 
               
               
                 AAAAGTCTAGGCACTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 369 LmW-43 F - ATACGGGAGCCAACACCACCCACTCTCCCCCCGCTCC 
               
               
                 CGCTCCCCCGCTCCGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 370 LmW-43 R - ATCCGTCACACCTGCTCTCGCGGAGCGGGGGAGCGGG 
               
               
                 AGCGGGGGGAGAGTGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 371 LmW-44 F - ATACGGGAGCCAACACCATCTAACAATCATACACTTG 
               
               
                 GAAGGTGACTGTCCTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 372 LmW-44 R - ATCCGTCACACCTGCTCTCCAGGACAGTCACCTTCCA 
               
               
                 AGTGTATGATTGTTAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 373 LmW-46 F - ATACGGGAGCCAACACCATGTCAGGACCTCCATCGCC 
               
               
                 CGGGCCCGCCGCCGCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 374 LmW-46 R - ATCCGTCACACCTGCTCTCAGCGGCGGCGGGCCCGGG 
               
               
                 CGATGGAGGTCCTGACATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 375 LmW-47 F - ATACGGGAGCCAACACCAGGCGACAGCCTGTGCGAGT 
               
               
                 AAGATTGAATGGTAGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 376 LmW-47 R - ATCCGTCACACCTGCTCTACCTACCATTCAATCTTACT 
               
               
                 CGCACAGGCTGTCGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 377 LmW-49 F - ATACGGGAGCCAACACCATCTGTGTCAGTCTGGCCTG 
               
               
                 TTTTTTATTCTCCGCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 378 LmW-49 R - ATCCGTCACACCTGCTCTCCGCGGAGAATAAAAAACA 
               
               
                 GGCCAGACTGACACAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 379 LmW-50 F - ATACGGGAGCCAACACCAGCCAGGAAAACTATGAGG 
               
               
                 CAAAAACACGATCCGGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 380 LmW-50 R - ATCCGTCACACCTGCTCTACCCGGATCGTGTTTTTGCC 
               
               
                 TCATAGTTTTCCTGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 N-acetyl-glucosamine (NAG) Component of Bacterial Peptidoglycan   
               
               
                 and Fungal Chitin 
               
               
                 SEQ ID NO. 381 NAG 13F - ATACGGGAGCCAACACCATAGAAGTATGTTGTTATTCTA 
               
               
                 TGGAAATAAAACGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 382 NAG 13R - ATCCGTCACACCTGCTCTGTCGTTTTATTTCCATAGAATA 
               
               
                 ACAACATACTTCTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 383 NAG 14F - ATACGGGAGCCAACACCATCCCGTTGTGATCAGAGAGC 
               
               
                 ATGAAATGATGTTTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 384 NAG 14R - ATCCGTCACACCTGCTCTCAAAACATCATTTCATGCTCT 
               
               
                 CTGATCACAACGGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 385 NAG 18F - ATACGGGAGCCAACACCATGCATGGGACCTGTTATCCTA 
               
               
                 ACAAGCTGTCAAGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 386 NAG 18R - ATCCGTCACACCTGCTCTGCCTTGACAGCTTGTTAGGAT 
               
               
                 AACAGGTCCCATGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 387 NAG 20F - ATACGGGAGCCAACACCACAAAACGTTCCGAGGGAGTA 
               
               
                 AGCACTTAATAATGTAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 388 NAG 20R - ATCCGTCACACCTGCTCTACATTATTAAGTGCTTACTCCC 
               
               
                 TCGGAACGTTTTGTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 389 NAG 21F - ATACGGGAGCCAACACCACGTCTTATAGATGTCTGTATT 
               
               
                 GTTTATCGCTCGCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 390 NAG 21R - ATCCGTCACACCTGCTCTGGGCGAGCGATAAACAATACA 
               
               
                 GACATCTATAAGACGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 391 NAG 22F - ATACGGGAGCCAACACCACCATCTCTGGTGATAACCAGT 
               
               
                 GATCTTAACTATAGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 392 NAG 22R - ATCCGTCACACCTGCTCTGCTATAGTTAAGATCACTGGT 
               
               
                 TATCACCAGAGATGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 393 NAG 23F - ATACGGGAGCCAACACCACCACCTCACTACAGTGATCTT 
               
               
                 TTGCTCTGAATAGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 394 NAG 23R - ATCCGTCACACCTGCTCTGGCTATTCAGAGCAAAAGATC 
               
               
                 ACTGTAGTGAGGTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 395 NAG 25F - ATACGGGAGCCAACACCATGTCTCTTAGGATACAAAGCC 
               
               
                 AAACTGAGCCCGTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 396 NAG 25R - ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTT 
               
               
                 GTATCCTAAGAGACATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 397 NAG 26F - ATACGGGAGCCAACACCACCTCCAATAGCCAAAAGAAA 
               
               
                 TCGCCAACTAACGGCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 398 NAG 26R - ATCCGTCACACCTGCTCTTGCCGTTAGTTGGCGATTTCTT 
               
               
                 TTGGCTATTGGAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 399 NAG 30F - ATACGGGAGCCAACACCATCACTACTTTTATAATTTCATT 
               
               
                 CTTCTGGCGTCCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 400 NAG 30R - ATCCGTCACACCTGCTCTAGGGACGCCAGAAGAATGAA 
               
               
                 ATTATAAAAGTAGTGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 N-acetyl-muramic acid (NAM) Component of Bacterial Peptidoglycan 
               
               
                 SEQ ID NO. 401 NAM 23F - ATACGGGAGCCAACACCAACTGCCCACGCCGCGACCCC 
               
               
                 GCGGCGCACCCAACCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 402 NAM 23R - ATCCGTCACACCTGCTCTTGGTTGGGTGCGCCGCGGGG 
               
               
                 TCGCGGCGTGGGCAGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 403 NAM 31F - ATACGGGAGCCAACACCAACGGTTACCAGGCGTGTTAA 
               
               
                 GGATATATGCTGAACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 404 NAM 31R - ATCCGTCACACCTGCTCTGGTTCAGCATATATCCTTAAC 
               
               
                 ACGCCTGGTAACCGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                   Salmonella typhimurium  lipopolysaccharide (LPS) DNA ligands 
               
               
                 SEQ ID NO. 405 (St-7F) - ATCCGTCACACCTGCTCTGTCCAAAGGCTACGCGTTAACGT 
               
               
                 GGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 406 (St-10F) - ATCCGTCACACCTGCTCTGGAGCAATATGGTGGAGAAACG 
               
               
                 TGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 407 (St-11F) - ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCT 
               
               
                 GTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 408 (St-15F) - ATCCGTCACACCTGCTCTGAACAGGATAGGGATTAGCGAG 
               
               
                 TCAACTAAGCAGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 409 (St-16F) - ATCCGTCACACCTGCTCTGGCGGACAGGAAATAAGAATG 
               
               
                 AACGCAAAATTTATCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 410 (St-18F) - ATCCGTCACACCTGCTCTACGCAACGCGACAGGAACATTC 
               
               
                 ATTATAGAATGTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 411 (St-19F) - ATCCGTCACACCTGCTCTCGGCTGCAATGCGGGAGAGTAG 
               
               
                 GGGGGAACCAAACCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 412 (St-20F) - ATCCGTCACACCTGCTCTATGACTGGAACACGGGTATCGA 
               
               
                 TGATTAGATGTCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 413 (St-7R) - ATACGGGAGCCAACACCACGTTAACGCGTAGCCTTTGGAC 
               
               
                 AGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 414 (St-10R) - ATACGGGAGCCAACACCACGTTTCTCCACCATATTGCTCC 
               
               
                 AGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 415 (St-11R) - ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCG 
               
               
                 GCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 416 (St-15R) - ATACGGGAGCCAACACCATGCTGCTTAGTTGACTCGCTAA 
               
               
                 TCCCTATCCTGTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 417 (St-16R) - ATACGGGAGCCAACACCAGATAAATTTTGCGTTCATTCTT 
               
               
                 ATTTCCTGTCCGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 418 (St-18R) - ATACGGGAGCCAACACCAACACATTCTATAATGAATGTT 
               
               
                 CCTGTCGCGTTGCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 419 (St-19R) - ATACGGGAGCCAACACCAGGTTTGGTTCCCCCCTACTCTC 
               
               
                 CCGCATTGCAGCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 420 (St-20R) - ATACGGGAGCCAACACCAAGGACATCTAATCATCGATAC 
               
               
                 CCGTGTTCCAGTCATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                   S. typhimurium  ( S. enterica  serovar  Typhimurium  type 13311)  
               
               
                 OMPs - Fresh Bacteria 
               
               
                 SEQ ID NO. 421 (StO-2F) - ATACGGGAGCCAACACCAGATAAATTTTGCGTTCATTCT 
               
               
                 TATTTCCTGTCCGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 422 (StO-2R) - ATCCGTCACACCTGCTCTGGCGGACAGGAAATAAGAAT 
               
               
                 GAACGCAAAATTTATCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 423 (StO-4F) - ATACGGGAGCCAACACCAGATAAATTTTGGTTCATTCTT 
               
               
                 ATTTCCTGTCCGCCAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 424 (StO-4R) - ATCCGTCACACCTGCTCTGGCGGACAGGAAATAAGAAT 
               
               
                 GAACCAAAATTTATCTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 425 (StO-5F) - ATACGGGAGCCAACACCACGGGGCTACCAGCACCGTCA 
               
               
                 CCCCTCATTCTGCCACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 426 (StO-5R) - ATCCGTCACACCTGCTCTGTGGCAGAATGAGGGGTGAC 
               
               
                 GGTGCTGGTAGCCCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 427 (StO-6F) - ATACGGGAGCCAACACCAAAAGATGGAAAACACTGGAA 
               
               
                 GGAAAATGCGGTCAGAGCAGGTGTGACGGAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 429 (StO-6R) - ATCCGTCACACCTGCTCTGACCGCATTTTCCTTCCAGTGT 
               
               
                 TTTCCATCTTTTGGTGTTGGCTCCCGTAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 429 (StO-7F) - ATACGGGAGCCAACACCACCGGGCCGATGGGCACCAGG 
               
               
                 AACTCTCGGACGAGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 430 (StO-7R) - ATCCGTCACACCTGCTCTCACTCGTCCGAGAGTTCCTGG 
               
               
                 TGCCCATCGGCCCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 431 (StO-8F) - ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCC 
               
               
                 GGCAGAGCAGGTGTGACGGAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 432 (StO-8R) - ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGC 
               
               
                 TGTGGTGTTGGCTCCCGTAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 433 (StO-9F) - ATACGGGAGCCAACACCAGTCGAAAGGCGGCCGTCCAG 
               
               
                 TCGAGTGATTTGACCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 434 (StO-9R) - ATCCGTCACACCTGCTCTAGGTCAAATCACTCGACTGGA 
               
               
                 CGGCCGCCTTTCGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 435 (StO-10F) - ATACGGGAGCCAACACCACGGGGCGTGCCGTCAAAAG 
               
               
                 ACCGAGATGTGGCTGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 436 (StO-10R) - ATCCGTCACACCTGCTCTCGCAGCCACATCTCGGTCTT 
               
               
                 TTGACGGCACGCCCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 437 (StO-11/13F) - ATACGGGAGCCAACACCACTAACTTGTTGCTGATCT 
               
               
                 TATCCAGAGCAGGTGTGACGGAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 438 (StO-11/13R) - ATCCGTCACACCTGCTCTGGATAAGATCAGCAACAA 
               
               
                 GTTAGTGGTGTTGGCTCCCGTAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 439 (StO-12F) - ATACGGGAGCCAACACCATTTAGCGTAGGGCTCGCTTA 
               
               
                 T CATTTCTCATTCCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 440 (StO-12R) - ATCCGTCACACCTGCTCTAGGGAATGAGAAATGATAAG 
               
               
                 CGAGCCCTACGCTAAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 441 (StO-14F) - ATACGGGAGCCAACACCACCGCAACCCAAATCTCTACA 
               
               
                 CGGATTATCGTCGAGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 442 (StO-14R) - ATCCGTCACACCTGCTCTGCTCGACGATAATCCGTGTA 
               
               
                 GAGATTTGGGTTGCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 443 (StO-16F) - ATACGGGAGCCAACACCAACACATTCTATAATGAATGT 
               
               
                 TCCTGTCGCGTTGCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 444 (StO-16R) - ATCCGTCACACCTGCTCTACGCAACGCGACAGGAACAT 
               
               
                 TCATTATAGAATGTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 445 (StO-17F) - ATACGGGAGCCAACACCAGCCTACCCCCCCTGTACGAG 
               
               
                 GGCCGCAACCACGTAGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 446 (StO-17R) - ATCCGTCACACCTGCTCTCTACGTGGTTGCGGCCCTCGT 
               
               
                 ACAGGGGGGGTAGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 447 (StO-18F) - ATACGGGAGCCAACACCACATCTAGCACGAGACCCTAT 
               
               
                 CCCAGAGCAGGTGTGACGGAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 448 (StO-18R) - ATCCGTCACACCTGCTCTGGGATAGGGTCTCGTGCTAG 
               
               
                 ATGTGGTGTTGGCTCCCGTAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 449 (StO-19F) - ATACGGGAGCCAACACCAACAGCGACTCGAGTCTGAC 
               
               
                 GACTCGCGGGGCAAATGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 450 (StO-19R) - ATCCGTCACACCTGCTCTCATTTGCCCCGCGAGTCGTC 
               
               
                 AGACTCGAGTCGCTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 451 (StO-20/24F) - ATACGGGAGCCAACACCATAGTGTTGGGCCAATACG 
               
               
                 GTAACGTGTCCTTGGAGAGCAGGTGTGACGGAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 452 (StO-20/24R) - ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGT 
               
               
                 ATTGGCCCAACACTATGGTGTTGGCTCCCGTAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 453 (StO-21F) - ATACGGGAGCCAACACCACTAAGGAGAGGTCGCGACA 
               
               
                 GACTCTTCTGGTCAAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 454 (StO-21R) - ATCCGTCACACCTGCTCTCCTTGACCAGAAGAGTCTGT 
               
               
                 CGCGACCTCTCCTTAGTGGTGTTGGCTCCCGTATG 
               
               
                   
               
               
                 SEQ ID NO. 455 (StO-22F) - ATACGGGAGCCAACACCAACTTCGACTCAAAGAAGTCC 
               
               
                 ACGTGAGACTGGTGGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 456 (StO-22R) - ATCCGTCACACCTGCTCTTCCACCAGTCTCACGTGGAC 
               
               
                 TTCTTTGAGTCGAAGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 457 (StO-23F) - ATACGGGAGCCAACACCACCCGGGGAGACCCGCACGG 
               
               
                 GCGCACAATCCTTGTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 458 (StO-23R) - ATCCGTCACACCTGCTCTCGACAAGGATTGTGCGCCCG 
               
               
                 TGCGGGTCTCCCCGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 459 (StO-25F) - ATACGGGAGCCAACACCAGCTGGACCAAACTACGCCC 
               
               
                 ATTGTGGGGGTCCCCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 460 (StO-25R) - ATCCGTCACACCTGCTCTCCGGGGACCCCCACAATGGG 
               
               
                 CGTAGTTTGGTCCAGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                   S. typhimurium  ( S. enterica  serovar  Typhimurium  type 13311)  
               
               
                 Whole Cell DNA Ligands 
               
               
                 SEQ ID NO. 461 StW-4/14/24/26/39/72 F - ATACGGGAGCCAACACCATAGTGTT 
               
               
                 GGGCCAATACGGTAACGTGTCCTTGGAGAGCAGGTGTGACGGAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 462 StW-4/14/24/26/39/72 R - ATCCGTCACACCTGCTCTCCAAGGAC 
               
               
                 ACGTTACCGTATTGGCCCAACACTATGGTGTTGGCTCCCGTAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 463 StW-7 F - ATACGGGAGCCAACACCAGTGGGACCTACGGCCTTTGG 
               
               
                 CCCGCTGTTACAACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 464 StW-7 R - ATCCGTCACACCTGCTCTACGTTGTAACAGCGGGCCAAA 
               
               
                 GGCCGTAGGTCCCACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 465 StW-9 F - ATACGGGAGCCAACACCACTTACGCATCAGCCACTCGA 
               
               
                 GAGACGGCGTTATGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 466 StW-9 R - ATCCGTCACACCTGCTCTGCCATAACGCCGTCTCTCGAG 
               
               
                 TGGCTGATGCGTAAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 467 StW-11 F - ATACGGGAGCCAACACCACTATAGGGTGTAGCTGATC 
               
               
                 CGCTCCCTTCTCCCAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 468 StW-11 R - ATCCGTCACACCTGCTCTCCTGGGAGAAGGGAGCGGAT 
               
               
                 CAGCTACACCCTATAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 469 StW-12 F - ATACGGGAGCCAACACCAGAACACCTAGAGACTAGTT 
               
               
                 CGTGTCGGCCCAGCGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 470 StW-12 R - ATCCGTCACACCTGCTCTCACGCTGGGCCGACACGAAC 
               
               
                 TAGTCTCTAGGTGTTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 471 StW-16 F - ATACGGGAGCCAACACCATAAGAACCACCATTCCGCGT 
               
               
                 TCGCCTCCCGAGGTGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 472 StW-16 R - ATCCGTCACACCTGCTCTACACCTCGGGAGGCGAACGC 
               
               
                 GGAATGGTGGTTCTTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 473 StW-19 F - ATACGGGAGCCAACACCAGGCCATAGGCAATTTCATAT 
               
               
                 AGCAACTGGTGAGCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 474 StW-19 R - ATCCGTCACACCTGCTCTACGCTCACCAGTTGCTATAT 
               
               
                 GAAATTGCCTATGGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 475 StW-20 F - ATACGGGAGCCAACACCAACAGAAGTCGACCCTGGTA 
               
               
                 ATCATGCTCTCTCACGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 476 StW-20 R - ATCCGTCACACCTGCTCTCCGTGAGAGAGCATGATTAC 
               
               
                 CAGGGTCGACTTCTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 477 StW-22 F - ATACGGGAGCCAACACCACCAACACCTGGAGAACTTG 
               
               
                 AAACGCAGATGGTCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 478 StW-22 R - ATCCGTCACACCTGCTCTGGGGACCATCTGCGTTTCAA 
               
               
                 GTTCTCCAGGTGTTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 479 StW-23 F - ATACGGGAGCCAACACCAGGTAGCGACATGACAGTAC 
               
               
                 CACTTACAGGACGTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 480 StW-23 R - ATCCGTCACACCTGCTCTGGCACGTCCTGTAAGTGGTA 
               
               
                 CTGTCATGTCGCTACCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 481 StW-25 F - ATACGGGAGCCAACACCAATGACGTAAACACAAACGG 
               
               
                 CGGACCCAATCGTGTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 482 StW-25 R - ATCCGTCACACCTGCTCTGAACACGATTGGGTCCGCCG 
               
               
                 TTTGTGTTTACGTCATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 483 StW-27 F - ATACGGGAGCCAACACCATGCTCCAGCATATTGATTAA 
               
               
                 TGCCAAGAGTTGGAACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 484 StW-27 R - ATCCGTCACACCTGCTCTGTTCCAACTCTTGGCATTAAT 
               
               
                 CAATATGCTGGAGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 485 StW-29 F - ATACGGGAGCCAACACCATGTGGTTCAGATGCGCCATA 
               
               
                 TCTAGACGGTCTCTGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 486 StW-29 R - ATCCGTCACACCTGCTCTACAGAGACCGTCTAGATATG 
               
               
                 GCGCATCTGAACCACATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 487 StW-30 F - ATACGGGAGCCAACACCAAACCCCATTCTGTCACAGCG 
               
               
                 CCACCCAACGAGTGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 488 StW-30 R - ATCCGTCACACCTGCTCTAACACTCGTTGGGTGGCGCT 
               
               
                 GTGACAGAATGGGGTTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 489 StW-34 F - ATACGGGAGCCAACACCAGCCGGTATCGGTGCTGAGGG 
               
               
                 CCTTGGCTTGGCTCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 489 StW-34 R - ATCCGTCACACCTGCTCTCAGAGCCAAGCCAAGGCCCT 
               
               
                 CAGCACCGATACCGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 490 StW-36 F - ATACGGGAGCCAACACCATGGCGACCTAATCAGCCGGA 
               
               
                 CAGTGCTCCTCAACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 491 StW-36 R - ATCCGTCACACCTGCTCTACGTTGAGGAGCACTGTCCG 
               
               
                 GCTGATTAGGTCGCCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 492 StW-38 F - ATACGGGAGCCAACACCATGGAGACAGGGGGAACGAC 
               
               
                 AGCGGCGGTTGCGGGGCAGAGCAGGTGTGACGGA 
               
               
                   
               
               
                 SEQ ID NO. 493 StW-38 R - ATCCGTCACACCTGCTCTGCCCCGCAACCGCCGCTGTC 
               
               
                 GTTCCCCCTGTCTCCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 494 StW-40 F - ATACGGGAGCCAACACCAATAGCCGGCCGAAATCCCTT 
               
               
                 TGGGATGGTCATACCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 495 StW-40 R - ATCCGTCACACCTGCTCTCGGTATGACCATCCCAAAGG 
               
               
                 GATTTCGGCCGGCTATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 496 StW-42 F - ATACGGGAGCCAACACCACCGAATGTGCTGCAAGACT 
               
               
                 AATCTGGATGGCCATGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 497 StW-42 R - ATCCGTCACACCTGCTCTGCATGGCCATCCAGATTAGTC 
               
               
                 TTGCAGCACATTCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 498 StW-43 F - ATACGGGAGCCAACACCAAATCGAGTTCGTGACAGTTG 
               
               
                 GGCAGATACCGAGTCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 499 StW-43 R - ATCCGTCACACCTGCTCTGGACTCGGTATCTGCCCAAC 
               
               
                 TGTCACGAACTCGATTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 500 StW-45 F - ATACGGGAGCCAACACCAGGGTCCACGCTACACGGATC 
               
               
                 AAGTCTAGCTGGTTGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 501 StW-45 R - ATCCGTCACACCTGCTCTACAACCAGCTAGACTTGATC 
               
               
                 CGTGTAGCGTGGACCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 502 StW-47 F - ATACGGGAGCCAACACCATCCCACAAGGCTCGTGTTAG 
               
               
                 GCCTCCAATGCTCTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 503 StW-47 R - ATCCGTCACACCTGCTCTCGAGAGCATTGGAGGCCTAA 
               
               
                 CACGAGCCTTGTGGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 504 StW-48 F - ATACGGGAGCCAACACCAGGCCCCGAGAAATTATCGAT 
               
               
                 AGTGGTTTCTCGCCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 505 StW-48 R - ATCCGTCACACCTGCTCTAGGGCGAGAAACCACTATCG 
               
               
                 ATAATTTCTCGGGGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 506 StW-49 F - ATACGGGAGCCAACACCACACCCGGATGCGATTAAGAA 
               
               
                 GTTACTGCCTTGCGGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 507 StW-49 R - ATCCGTCACACCTGCTCTCCCGCAAGGCAGTAACTTCTT 
               
               
                 AATCGCATCCGGGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 508 StW-50 F - ATACGGGAGCCAACACCATGCCATGCACTTGGTTCCGA 
               
               
                 ACGTTCGCGTCATTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 509 StW-50 R - ATCCGTCACACCTGCTCTGCAATGACGCGAACGTTCGG 
               
               
                 AACCAAGTGCATGGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 510 StW-56 F - ATACGGGAGCCAACACCACCAAAAAAAGCTGTGACCG 
               
               
                 GAAGGTGCTGCTGACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 511 StW-56 R - ATCCGTCACACCTGCTCTACGTCAGCAGCACCTTCCGGT 
               
               
                 CACAGCTTTTTTTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 512 StW-58 F - ATACGGGAGCCAACACCAAGCTACCATCCACCTAACAG 
               
               
                 GACTACGCGAATTGCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 513 StW-58 R - ATCCGTCACACCTGCTCTTGCAATTCGCGTAGTCCTGTT 
               
               
                 AGGTGGATGGTAGCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 514 StW-61 F - ATACGGGAGCCAACACCACAAGCAGGAATAAGCGCCG 
               
               
                 GTCCAGAGCAGGTGTGACGGAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 515 StW-61 R - ATCCGTCACACCTGCTCTGGACCGGCGCTTATTCCTGCT 
               
               
                 TGTGGTGTTGGCTCCCGTAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 516 StW-62 F - ATACGGGAGCCAACACCACATGGACCGGCAACCTCAG 
               
               
                 AAGTAGCAAACCACCATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 517 StW-62 R - ATCCGTCACACCTGCTCTATGGTGGTTTGCTACTTCTGA 
               
               
                 GGTTGCCGGTCCATGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 518 StW-65 F - ATACGGGAGCCAACACCATGTCCAAACCATTCTCGGAC 
               
               
                 CTCCCTCAGTGGCGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 519 StW-65 R - ATCCGTCACACCTGCTCTGCCGCCACTGAGGGAGGTCC 
               
               
                 GAGAATGGTTTGGACATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 520 StW-66 F - ATACGGGAGCCAACACCAGTCCGTTATGACATGTCCGG 
               
               
                 ACCCGTACGCGTGTCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 521 StW-66 R - ATCCGTCACACCTGCTCTTGACACGCGTACGGGTCCGG 
               
               
                 ACATGTCATAACGGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 522 StW-67 F - ATACGGGAGCCAACACCATCCGCTCACATGATGCTGTA 
               
               
                 CGATGGCCGCGTGCAAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 523 StW-67 R - ATCCGTCACACCTGCTCTTTGCACGCGGCCATCGTACA 
               
               
                 GCATCATGTGAGCGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 524 StW-68 F - ATACGGGAGCCAACACCACGTCGCATATACCCCGAGAA 
               
               
                 GGTAGATCGTGGACTAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 525 StW-68 R - ATCCGTCACACCTGCTCTAGTCCACGATCTACCTTCTCG 
               
               
                 GGGTATATGCGACGTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 526 StW-69 F - ATACGGGAGCCAACACCACGAGGACCTAGACTTGTCCG 
               
               
                 ACATCACAGTGTGCGAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 527 StW-69 R - ATCCGTCACACCTGCTCTCGCACACTGTGATGTCGGAC 
               
               
                 AAGTCTAGGTCCTCGTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 528 StW-70 F - ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCC 
               
               
                 GGCAGAGCAGGTGTGACGGAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 529 StW-70 R - ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAG 
               
               
                 CTGTGGTGTTGGCTCCCGTAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 530 StW-71 F - ATACGGGAGCCAACACCACGGGACCATCAGCCTCAACT 
               
               
                 TCCTACAAGGCCTACTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 531 StW-71 R - ATCCGTCACACCTGCTCTAGTAGGCCTTGTAGGAAGTT 
               
               
                 GAGGCTGATGGTCCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 532 StW-73 F - ATACGGGAGCCAACACCAATGGACAAAGGCAATAGCG 
               
               
                 TCAATTGAAGTCAGACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 533 StW-73 R - ATCCGTCACACCTGCTCTGGTCTGACTTCAATTGACGCT 
               
               
                 ATTGCCTTTGTCCATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 534 StW-74 F - ATACGGGAGCCAACACCAACTGAACTCATGAAGCACG 
               
               
                 ATTGTTGCCCCACGTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 535 StW-74 R - ATCCGTCACACCTGCTCTGCACGTGGGGCAACAATCGT 
               
               
                 GCTTCATGAGTTCAGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 536 StW-76 F - ATACGGGAGCCAACACCAATCCCTAGCAAGTAAGCTGG 
               
               
                 TGGAGCTAGTACACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 537 StW-76 R - ATCCGTCACACCTGCTCTACGTGTACTAGCTCCACCAGC 
               
               
                 TTACTTGCTAGGGATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 538 StW-78 F - ATACGGGAGCCAACACCACACCGAAAGCCGGAACGAT 
               
               
                 AGGGTACAGCTGGGTGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 539 StW-78 R - ATCCGTCACACCTGCTCTACACCCAGCTGTACCCTATC 
               
               
                 GTTCCGGCTTTCGGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 540 StW-79 F - ATACGGGAGCCAACACCAAGGGCGAACTAGCATCACC 
               
               
                 TCGGTCGCTCATAGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 541 StW-79 R - ATCCGTCACACCTGCTCTGGCCTATGAGCGACCGAGGT 
               
               
                 GATGCTAGTTCGCCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 542 StW-80 F - ATACGGGAGCCAACACCACAGGGCGACGTAAGCTCCG 
               
               
                 TCCAGAGGATGTCAGTAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 543 StW-80 R - ATCCGTCACACCTGCTCTACTGACATCCTCTGGACGGA 
               
               
                 GCTTACGTCGCCCTGTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 Shiga-like Toxin type 1; Stx-1 
               
               
                 SEQ ID NO. 544 (SH-2F) - ATCCGTCACACCTGCTCTGGAGACATTAAAAACCGGAG 
               
               
                 TTTATTTATACCTTTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 545 (SH-2R) - ATACGGGAGCCAACACCAGAAAGGTATAAATAAACTCC 
               
               
                 GGTTTTTAATGTCTCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 546 (SH-3F (59)) - ATACGGGAGCCAACACCACTAACTTGTTGCTGATCT 
               
               
                 TATCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 547 (SH-3R (59)) - ATCCGTCACACCTGCTCTGGATAAGATCAGCAACAA 
               
               
                 GTTAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 548 (SH-4F (58)) - ATCCGTCACACCTGCTCTGCATGGAGAGTTTTTTGGT 
               
               
                 CAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 549 (SH-4R (58)) - ATACGGGAGCCAACACCACTGACCAAAAAACTCTC 
               
               
                 CATGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 550 (SH-6F (58)) - ATACGGGAGCCAACACCACGTTAACGCGTAGCCTTT 
               
               
                 GGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 551 (SH-6R (58)) - ATCCGTCACACCTGCTCTGTCCAAAGGCTACGCGTT 
               
               
                 AACGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 552 (SH-8/21/23/24/25F (59)) - ATCCGTCACACCTGCTCTGCCGG 
               
               
                 ACCATCCAATATCAGCTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 553 (SH-8/21/23/24/25 Rev (59)) - ATACGGGAGCCAACACCACA 
               
               
                 GCTGATATTGGATGGTCCGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 554 (SH-9F) - ATCCGTCACACCTGCTCTCGTCCGTCATTAAGTTCGGAG 
               
               
                 GCTGGCGGGTTGCGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 555 (SH-9R) - ATACGGGAGCCAACACCAACGCAACCCGCCAGCCTCCG 
               
               
                 AACTTAATGACGGACGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 556 (SH-10F) - ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTT 
               
               
                 ATGCCTTGCCATCTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 557 (SH-10R) - ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGC 
               
               
                 GTCCGGAACGATAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 558 (SH-11F) - TCCGTCACACCTGCTCTAACTCTTACTACTTTGTTGCTA 
               
               
                 TCACATTCAACTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 559 (SH-11R) - ATACGGGAGCCAACACCAACAGTTGAATGTGATAGCA 
               
               
                 ACAAAGTAGTAAGAGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 560 (SH-12 F(58)) - ATCCGTCACACCTGCTCTGGCCTTTCACCAAGCG 
               
               
                 TCCTTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 561 (SH-12R (58)) - ATACGGGAGCCAACACCACAAGGACGCTTGGTGAA 
               
               
                 AGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 562 (SH-16F (58)) - ATCCGTCACACCTGCTCTGGCACCGAGCACGGGAA 
               
               
                 CCCAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 563 (SH-16R (58)) - ATACGGGAGCCAACACCACTGGGTTCCCGTGCTCG 
               
               
                 GTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 564 (SH-17F (69)) - ATACGGGAGCCAACACCATAGTGTTGGGCCAATAC 
               
               
                 GGTAACGTGTCCTTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 565 (SH-17R (69)) - ATCCGTCACACCTGCTCTCCAAGGACACGTTACCG 
               
               
                 TATTGGCCCAACACTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 567 (SH-18F) - ATCCGTCACACCTGCTCTACCCGATGCCGCCCCGGGATT 
               
               
                 GTTGTATGACCATCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 568 (SH-18R) - ATACGGGAGCCAACACCAAGATGGTCATACAACAATC 
               
               
                 CCGGGGCGGCATCGGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 569 (SH-19F) - ATACGGGAGCCAACACCACCCCATGAGTACACGTGAAC 
               
               
                 GGACACAGCCTCCGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 570 (SH-19R) - ATCCGTCACACCTGCTCTGCCGGAGGCTGTGTCCGTTC 
               
               
                 ACGTGTACTCATGGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 571 (SH-20F) - ATCCGTCACACCTGCTCTTAACCATTCATTTCTTTTGTG 
               
               
                 GTATGACCGTTCGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 572 (SH-20R) - ATACGGGAGCCAACACCAGGCGAACGGTCATACCACA 
               
               
                 AAAGAAATGAATGGTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 573 (SH-22F (58)) - ATCCGTCACACCTGCTCTGGGGCTCTTTTCGTTA 
               
               
                 ACCAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 574 (SH-22R (58)) - ATACGGGAGCCAACACCACCTGGTTAACGAAAAGA 
               
               
                 GCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 Shiga-like toxin 2; Stx-2 
               
               
                 SEQ ID NO. 575 S2-1 F - ATACGGGAGCCAACACCAGGCGACCAAGTTTGAATCACC 
               
               
                 ACAATCGTGACGGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 576 S2-1 R - ATCCGTCACACCTGCTCTCACCGTCACGATTGTGGTGATTC 
               
               
                 AAACTTGGTCGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 577 S2-2 F - ATACGGGAGCCAACACCACCATCACATCTTGGCCCGGTAC 
               
               
                 CCTGGATACTAGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 578 S2-2 R - ATCCGTCACACCTGCTCTGGCTAGTATCCAGGGTACCGGG 
               
               
                 CCAAGATGTGATGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 579 S2-3 F - ATACGGGAGCCAACACCAGCACTAGCTCGGGTAACGGGG 
               
               
                 ACATTAGAGTTTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 580 S2-3 R - ATCCGTCACACCTGCTCTGGCAAACTCTAATGTCCCCGTT 
               
               
                 ACCCGAGCTAGTGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 581 S2-4 F - ATACGGGAGCCAACACCAAAGCCCACCGCGCCCAGATCT 
               
               
                 ACAAGACTTCCAACTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 582 S2-4 R - ATCCGTCACACCTGCTCTAGTTGGAAGTCTTGTAGATCTG 
               
               
                 GGCGCGGTGGGCTTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 583 S2-5 F - ATACGGGAGCCAACACCATCTTTGTCACTCTGGATTAGGT 
               
               
                 TAATCCACTGAAACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 584 S2-5 R - ATCCGTCACACCTGCTCTGTTTCAGTGGATTAACCTAATC 
               
               
                 CAGAGTGACAAAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 585 S2-7 F - ATACGGGAGCCAACACCACGAACCCGGGATTCTAGCAATT 
               
               
                 GTCCCCCTCGAGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 586 S2-7 R - ATCCGTCACACCTGCTCTCGCTCGAGGGGGACAATTGCTA 
               
               
                 GAATCCCGGGTTCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 587 S2-8 F - ATACGGGAGCCAACACCAATGATTAATAGAACCCCCTAT 
               
               
                 GACCTGGCCGCTGGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 588 S2-8 R - ATCCGTCACACCTGCTCTCCCAGCGGCCAGGTCATAGGGG 
               
               
                 GTTCTATTAATCATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 589 S2-9 F - ATACGGGAGCCAACACCATGGTCGGATAGCATGTCCATG 
               
               
                 TTGTCGGGTTTAACAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 590 S2-9 R - ATCCGTCACACCTGCTCTTGTTAAACCCGACAACATGGAC 
               
               
                 ATGCTATCCGACCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 591 S2-10 F - ATACGGGAGCCAACACCAGGGGAATCTTGCTTGCGTAGC 
               
               
                 GACGCATAATGACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 592 S2-10 R - ATCCGTCACACCTGCTCTACGTCATTATGCGTCGCTACG 
               
               
                 CAAGCAAGATTCCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 593 S2-12 F - ATACGGGAGCCAACACCATGAAGTGGACAAATGTGCGTT 
               
               
                 CCCCTGACGTACCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 594 S2-12 R - ATCCGTCACACCTGCTCTCCGGTACGTCAGGGGAACGCA 
               
               
                 CATTTGTCCACTTCATGGTGTTGGCTCCCGTA 
               
               
                   
               
               
                 SEQ ID NO. 595 S2-13 F - ATACGGGAGCCAACACCACCATTTAGTGTTAGACTAAGT 
               
               
                 GATATCGAGTCGAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 596 S2-13 R - ATCCGTCACACCTGCTCTCCTCGACTCGATATCACTTAGT 
               
               
                 CTAACACTAAATGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 597 S2-14 F - ATACGGGAGCCAACACCACTTCCACTTTTTCGCCTAATT 
               
               
                 GCCTGTTGCATGGTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 598 S2-14 R - ATCCGTCACACCTGCTCTTACCATGCAACAGGCAATTAG 
               
               
                 GCGAAAAAGTGGAAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 599 S2-14.1 F - ATACGGGAGCCAACACCAGGCGATGTCCTAAAGTCTTT 
               
               
                 AAGGCGAATATAGTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 600 S2-14.1 R - ATCCGTCACACCTGCTCTCAACTATATTCGCCTTAAAGA 
               
               
                 CTTTAGGACATCGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 601 S2-15 F - ATACGGGAGCCAACACCACCCCCCCCTCCGTGGGCCGCT 
               
               
                 CCCCTCGGCCGGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 602 S2-15 R - ATCCGTCACACCTGCTCTGGCCCGGCCGAGGGGAGCGGC 
               
               
                 CCACGGAGGGGGGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 603 S2-16 F - ATACGGGAGCCAACACCATCCCGTGAAGCAACGACAATA 
               
               
                 CAAGACGAGCGAAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 604 S2-16 R - ATCCGTCACACCTGCTCTCCTTCGCTCGTCTTGTATTGTC 
               
               
                 GTTGCTTCACGGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 605 S2-17 F - ATACGGGAGCCAACACCACGCGACTTCTTCAACAGATAC 
               
               
                 AGAGCGCTTGGGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 606 S2-17 R - ATCCGTCACACCTGCTCTGGCCCCAAGCGCTCTGTATCT 
               
               
                 GTTGAAGAAGTCGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 607 S2-18 F - ATACGGGAGCCAACACCAGGAAATGGTACCTAAGAAAT 
               
               
                 GAGAACTTTGACGCACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 607 S2-18 R - ATCCGTCACACCTGCTCTGTGCGTCAAAGTTCTCATTTCT 
               
               
                 TAGGTACCATTTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 609 S2-19 F - ATACGGGAGCCAACACCATTAAAGTTAATCTTACACGTT 
               
               
                 TCCGACTTCCATTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 610 S2-19 R - ATCCGTCACACCTGCTCTCAAATGGAAGTCGGAAACGTG 
               
               
                 TAAGATTAACTTTAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 611 S2-20 F - ATACGGGAGCCAACACCAAGGAGTCCGTCTACGTTTTAC 
               
               
                 GAGCTAAGGCCTTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 612 S2-20 R - ATCCGTCACACCTGCTCTCAAAGGCCTTAGCTCGTAAAA 
               
               
                 CGTAGACGGACTCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                   Cryptosporidium parvum  oocysts (CP) 
               
               
                 SEQ ID NO. 613 CP 12F - ATACGGGAGCCAACACCATAATGAAGCGATGTAGCGAGTT 
               
               
                 TTTGAAAGGGACACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 614 CP12R - ATCCGTCACACCTGCTCTGTGTCCCTTTCAAAAACTCGCTA 
               
               
                 CATCGCTTCATTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 615 CP 13F - ATACGGGAGCCAACACCATTTAGTCCATAGCTTCAGCGCT 
               
               
                 TCCACCTCCTTAACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 616 CP 13R - ATCCGTCACACCTGCTCTGTTAAGGAGGTGGAAGCGCTGA 
               
               
                 AGCTATGGACTAAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 617 CP 15F - ATACGGGAGCCAACACCACCCGTTTTTGATCTAATGAGGA 
               
               
                 TACAATATTCGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 618 CP 15R - ATCCGTCACACCTGCTCTAGACGAATATTGTATCCTCATTA 
               
               
                 GATCAAAAACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 619 CP 16F - ATACGGGAGCCAACACCACCGGGTCCCCGTGATCTAGGAC 
               
               
                 AACACGGCGGTTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 620 CP 16R - ATCCGTCACACCTGCTCTCCAACCGCCGTGTTGTCCTAGAT 
               
               
                 CACGGGGACCCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 621 CP 17F - ATACGGGAGCCAACACCAGTTCAGGCATACATGATGTGGG 
               
               
                 TTCTTATTCCGTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 622 CP 17R - ATCCGTCACACCTGCTCTGCACGGAATAAGAACCCACATC 
               
               
                 ATGTATGCCTGAACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 623 CP18F - ATACGGGAGCCAACACCAGGCAGCCCGGTCCCGGACTAAC 
               
               
                 AACCGCGGTACCCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 624 CP18R - ATCCGTCACACCTGCTCTTGGGTACCGCGGTTGTTAGTCCG 
               
               
                 GGACCGGGCTGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 625 CP20F - ATACGGGAGCCAACACCATTCAGGGCTTTTGTGTATGCAC 
               
               
                 TCCAGCTATCAGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 626 CP20R - ATCCGTCACACCTGCTCTGTCTGATAGCTGGAGTGCATACA 
               
               
                 CAAAAGCCCTGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 627 CP 21F - ATACGGGAGCCAACACCAAGGGACGGCAGGTTCGCAGCT 
               
               
                 GCGTCATCTTTCTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 628 CP 21R - ATCCGTCACACCTGCTCTGAAGAAAGATGACGCAGCTGCG 
               
               
                 AACCTGCCGTCCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 629 CP 22F (71) - ATACGGGAGCCAACACCACGAGGACTTAGACTTGTCC 
               
               
                 GACATCACAGTGTGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 630 CP 22R (71) - ATCCGTCACACCTGCTCTCGCACACTGTGATGTCGG 
               
               
                 ACAAGTCTAAGTCCTCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 631 CP 23F - ATACGGGAGCCAACACCACTTCCCTGTCCTTCCCTCAGTG 
               
               
                 AGGCCTGTCTCCTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 632 CP 23R - ATCCGTCACACCTGCTCTGAGGAGACAGGCCTCACTGAGG 
               
               
                 GAAGGACAGGGAAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 633 CP 24F - ATACGGGAGCCAACACCAGGAGATGTTCGTGTAATAGGGG 
               
               
                 GTTACACCCGGTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 634 CP 24R - ATCCGTCACACCTGCTCTCGACCGGGTGTAACCCCCTATTA 
               
               
                 CACGAACATCTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 635 CP 25F - ATACGGGAGCCAACACCATCGCTCAAGTTCTTCATTACTCC 
               
               
                 TATCGCTTCCGCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 636 CP 25R - ATCCGTCACACCTGCTCTAGCGGAAGCGATAGGAGTAATG 
               
               
                 AAGAACTTGAGCGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                   Giardia  (UDP-N-acetylgalactosamine Surface Antigen; Gi) 
               
               
                 SEQ ID NO. 637 Gi 22F - ATACGGGAGCCAACACCATTCTACTCCCAGGTATGTCTCTG 
               
               
                 GGCCCCCCCGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 638 Gi 22R - ATCCGTCACACCTGCTCTGGCCGGGGGGGCCCAGAGACAT 
               
               
                 ACCTGGGAGTAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 639 Gi 25F - ATACGGGAGCCAACACCAACAACATAGCCCTGGCACGAC 
               
               
                 AGTGGCATACCAGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 640 Gi 25R - ATCCGTCACACCTGCTCTGCCTGGTATGCCACTGTCGTGCC 
               
               
                 AGGGCTATGTTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 641 Gi 30F - ATACGGGAGCCAACACCACGTAATGATGTGCACCTCTCTC 
               
               
                 CGACTGTTTCTCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 642 Gi 30R - ATCCGTCACACCTGCTCTACGAGAAACAGTCGGAGAGAGG 
               
               
                 TGCACATCATTACGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 643 Gi-33F - ATACGGGAGCCAACACCACATCTTATTCGTCCCCAGTCCT 
               
               
                 TTGGTCTCCTGCTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 644 Gi-33R - TCCGTCACACCTGCTCTGAGCAGGAGACCAAAGGACTG 
               
               
                 GGGACGAATAAGATGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 645 Gi-37F - ATACGGGAGCCAACACCACCTGCTGATTTCCTATAATCC 
               
               
                 GGCCCATACCTTAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 646 Gi-37R - ATCCGTCACACCTGCTCTCCTAAGGTATGGGCCGGATTA 
               
               
                 TAGGAAATCAGCAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 647 Gi-38F - ATACGGGAGCCAACACCATAAGAGTCCTCTAAGGTCGCT 
               
               
                 TATTTTTAACCCCTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 648 Gi-38R - ATCCGTCACACCTGCTCTTAGGGGTTAAAAATAAGCGAC 
               
               
                 CTTAGAGGACTCTTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 649 Gi-40F - ATACGGGAGCCAACACCATCCCCACACCCTCGTTCCGACC 
               
               
                 GCTAGAATCCCCGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 650 Gi-40R - ATCCGTCACACCTGCTCTTCGGGGATTCTAGCGGTCGGAA 
               
               
                 CGAGGGTGTGGGGATGGTGTTGGCTCCCGTAT