Abstract:
The invention provides for a universal genetic evaluation system capable of simultaneously determining multiple genetic characteristics in domestic and wild animals. In particular, the invention provides for the use of polymorphisms, such as single nucleotide polymorphisms (SNPs), insertions, deletions, inversions, and/or other mutations within gene sequences, as determinants of genetic characteristics, such as parentage, identity, sex, genotype and/or phenotype. The universal genetic evaluation system is utilized to simultaneously determine multiple genetic characteristics in horses and wild horses, dogs and wild canids, cats, goats and wild goats, sheep and wild sheep, cattle, bison, deer (cervidae), donkeys, mules, swine and wild swine, camelids and wild camelids, other domestic and certain species of wild animals (deer, elk, red deer, antelope, caribou and reindeer, moose and other exotic deer and antelope species), birds (including pet birds and commercial bird species), reptiles, amphibians, fish and rodents, concurrently for each species.

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
       [0001]    The present invention claims benefit of priority to U.S. patent application Ser. No. 60/935,298 filed on Aug. 3, 2007, the contents of which are herein incorporated by reference in their entirety. 
     
    
     FIELD OF THE INVENTION 
       [0002]    The invention relates to a universal genetic evaluation system capable of simultaneously determining multiple genetic characteristics in domestic and wild animals. In particular, the invention provides for the concurrent detection of polymorphisms, such as single nucleotide polymorphisms (SNPs), insertions and/or deletions and other mutations within gene sequences, as determinants of genetic characteristics, such as parentage, identity, sex, genotype and/or phenotype and breed determination, and providing corresponding profiles. 
       BACKGROUND OF THE INVENTION 
       [0003]    The present invention provides for a universal genetic evaluation system capable of simultaneously determining multiple genetic characteristics in domestic animal. This universal system for identification and determination of key characteristics of individual animals maximizes their individual potential performance and traits as well as health and facilitates management and care of individual animals. The invention methods allow predictive (predisposition) diagnostics, character and trait determination such that nutritional therapies and pharmaceutical therapeutics can be administered to domestic animals when and if appropriate. Traits determined by the invention can be utilized to promote selective breeding to increase the value of the animals tested. The methods of the invention provide systems to collect, record, analyze and store data associated with multiple genetic characteristics in individual animals so that the data is usable to improve future performance, desirable traits and health of animals. The methods and systems of the present invention utilize information regarding genetic diversity among domestic and wild animals, particularly single nucleotide polymorphisms (SNPs), insertions, deletions, inversions and other mutations, and then correlate the presence of SNPs, insertions, deletions and other mutations of selected nucleotide marker sequences with important characteristics such as parentage, identity, sex, genotype and phenotype of domestic and wild animals. 
         [0004]    The present invention is based, in part, on the discovery of domestic and wild animal markers containing mutations, including but not limited to, single nucleotide polymorphisms (SNP), insertions, deletions or inversions that can be utilized to identify individual animals, determine or verify parentage of a single animal from any breed, and predict or determine phenotype and/or genotype. Specifically, the present invention provides compositions, methods and systems for the identification of at least two characteristics, where the characteristics are parentage, breed, identity as well as forensic identity, sex, genotype and/or phenotype. These compositions, methods and systems aid in management of individual animals or groups of animals to maximize their individual potential performance and health, and are important with respect to livestock evaluation. Compositions, methods and systems of the present invention utilized to determine parentage and identity can be used to:
       1) assign or verify parentage in disputed cases or as a quality control check for breed registries or for breed certification. These panels are currently utilized by domestic animal breed registries for verifying parentage of a defined set of parents and progeny;   2) match and verify the identity of a lost or stolen animal or to verify the identity of unknown evidentiary samples when compared to a known animal sample. When combined with a database of genotypes and animals, the panel can be used to match unknown animals to itself, if a genotype has been previously recorded, or to parents and siblings;   3) verify the identity of a cloned animal or frozen or split and/or cloned embryo;   4) verify the identity of banked and/or frozen semen, or verify cultured cell lines; and   5) link an known animal, animal hair or animal biological samples to a crime scene evidentiary sample for forensic applications.       
 
         [0010]    DNA analysis provides a powerful tool for determining the parentage, breed, identity and/or phenotype of individual animals. Microsatellite marker panels have been developed for cattle (Sherman et al.,  Anim Genet.  35(3):220-6; Heyen et al.,  Arnim Genet.  28(1):21-27) and canine (See e.g., U.S. Pat. No. 5,874,217; Ostrander et al.,  Mammalian Genome,  6: 192-195; Franscisco et al., Mammalian Genome 7:359-362) that are highly polymorphic and amenable to standardization among laboratories performing these tests. However, microsatellite scoring requires considerable human oversight and microsatellite markers have high mutation rates. Single nucleotide polymorphisms (SNP) have also been utilized because of the ease of scoring, low cost assay development and high-throughput capability. There have been limited studies to evaluate the usefulness of SNP markers in small populations of animals (Heaton et al.,  Mamm Genome.  13(5):272-81; Werner et al.,  Anim. Genet.  35(1):44-9). In addition, the utilization of SNPs alone does not provide coverage for certain important nucleotide marker polymorphisms of interest. 
         [0011]    Parentage and identity panels are the first applied technology of using genomic analysis to begin managing domestic animals. For example, panels have been developed utilizing microsatellite marker panels (DeNise et al., 2004 . Anim. Genetics.  35(1): 14-17; Halverson et al., 1995. U.S. Pat. No. 5,874,217; Ostrander et al., 1993 . Genomics  16: 207-213, Ostrander et al., 1995 . Mammalian Genome,  6: 192-195; Franscisco et al., 1996 . Mammalian Genome  7:359-362. 
         [0012]    Compared with other types of DNA markers, single nucleotide polymorphisms (SNPs) are attractive because they are abundant, genetically stable, and amenable to high-throughput automated analysis. In animal husbandry and the management of health and performance, one challenge has been the development of a cost-efficient system to simultaneously identify parentage, breed, identity and phenotype. Another challenge has been the development of a system that can be applied to more than genera or species of animal, e.g., a universal system that can be utilized to identify parentage, breed, identity and phenotype in horse, cattle, dogs, cats, sheep, goat, bison, deer, elk, antelope, caribou, reindeer, moose, donkeys, mules, swine, camelids and other domestic and wild animals. A further challenge has been the identification of a minimal set of SNPs with sufficient power to identify parentage, identity, sex, genotype and phenotype simultaneously in one species of animal, and a minimal set of SNPs with sufficient power to identify parentage, identity, sex, genotype and phenotype in more than one species of animal. 
         [0013]    Accordingly, there remains a need in the art for compositions, methods and systems that provide for cost-efficient analysis where at least two characteristics selected from the group consisting of parentage, identity, sex, genotype and phenotype can be simultaneously identified in an animal, or more than one species of animal. In addition, there remains a need in the art for compositions, methods and systems that are capable of providing this type of analysis by utilizing various polymorphic nucleotide marker sequences, including nucleotide marker sequences have single nucleotide polymorphisms (SNPs), insertions and/or deletions or other mutations at their polymorphic sites. 
       BRIEF SUMMARY OF THE INVENTION 
       [0014]    The present invention provides a method for simultaneously identifying a plurality of polymorphisms in a nucleic acid sample isolated from an animal comprising the steps of: (a) placing said nucleic acid sample in at least two recesses of an assay plate; (b) hybridizing said nucleic acid sample to a pair of forward and reverse primers; (c) contacting said nucleic acid sample with a first oligonucleotide probe and with a second oligonucleotide probe; (c) performing PCR amplification; and (d) detecting the presence of said plurality of polymorphisms in said nucleic acid sample. 
         [0015]    In specific embodiments of the invention, the first oligonucleotide probe is capable of detecting a first allele of a nucleotide marker sequence and the second oligonucleotide probe is capable of detecting a second allele of a nucleotide marker sequence; wherein the nucleotide marker sequence is any one of the nucleotide marker sequences as set forth in Tables 1-11; and wherein said nucleotide marker sequence correlates with at least one of the characteristics of an animal selected from the group consisting of: (i) parentage; (ii) identity; (iii) sex (iv) genotype and (v) phenotype; and wherein said method is capable of simultaneously identifying at least two characteristics of said animal selected from the group consisting of: (i) parentage; (ii) identity; (iii) sex (iv) genotype and (v) phenotype. 
         [0016]    In certain embodiments of the invention, the plurality of polymorphisms correlates with all five characteristics. In other embodiments of the invention, the plurality of polymorphisms is simultaneously identified in more than one nucleic acid sample, where each of the nucleic acid samples can be isolated from more than one individual animal of the same species, or different species. 
         [0017]    In other embodiments of the invention the nucleic acid sample is isolated from an animal, where the animal is of a family selected from the group consisting of Equidae, Bovidae, Canidae, and Felidae. In further embodiments, animals of the family Bovidae are of a species selected from the group consisting of  Bos, Ovis , and  Capra . In further embodiments, animals of the family Equidae are of a species selected from the group consisting of  Equus . In further embodiments, animals of the family Canidae are of a species selected from the group consisting of  Canis . In further embodiments, animals of the family Felidae are of a species selected from the group consisting of  Felis.    
         [0018]    In other embodiments of the invention, the plurality of polymorphisms comprises between about 20 and about 10,000 polymorphisms and extending up to whole genome analysis, between about 20 and about 3000 polymorphisms, between about 20 and 200 polymorphisms. In further embodiments, the plurality of polymorphisms comprises about 60, 100, 3000, 6000 or 9000 polymorphisms, about 64, 128, 3072, 6344 or 9216 polymorphisms, or about 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 polymorphisms. 
         [0019]    In preferred embodiments, the plurality of polymorphisms comprises the polymorphisms associated with each of the nucleotide marker sequence according to Tables 2, 4, 6, 8 and/or 11. 
         [0020]    In certain other embodiments, each of the primers of the invention is about 8 to about 30 nucleotides in length. 
         [0021]    In certain embodiments of the invention, the phenotype is a trait. In further embodiments, the trait is selected from the group consisting of coat color, hair color, hair length, eye color, marbling, tenderness, quality grade, muscle content, fat thickness, feed efficiency, red meat yield, average daily weight gain, disease resistance, disease susceptibility, feed intake, protein content, bone content, maintenance energy requirement, mature size, amino acid profile, fatty acid profile, milk production, a milk quality susceptibility to the buller syndrome, stress susceptibility and response, temperament, digestive capacity, production of calpain, caplastatin and myostatin, pattern of fat deposition, ribeye area, fertility, ovulation rate, conception rate, fertility, and susceptibility to infection with and shedding of pathogens. In certain other embodiments, the trait is a coat color is selected from the group consisting of cream, silver, tobiano, sabino, agouti, chestnut, brown, dilution, melanistic mask, albinism, recessive black, points, Burmese shading, cinnamon, red, and merle. 
         [0022]    In certain embodiments of the invention, the phenotype correlates with a disease. In further embodiments, the disease is selected from the group consisting of Lethal White Overo syndrome (LWO), Glycogen Branching Enzyme deficiency (GBE1), junctional epidermolysis bullosa (JEB), Severe Combined Immune Deficiency Syndrome (SCID), and Hyperkalemic Periodic Paralysis (HYPP). In additional embodiments, the disease is selected from the group consisting of congenital myotonia, muscular dystrophy, globoid cell leukodystrophy, GM-gangliosidosis, Hemophilia B, hereditary cataracts, phosphofructokinase deficiency, thrombasthenic thrombopathia, retinal dystrophy, type-2 von Willebrand&#39;s disease, and Type III von Willebrand. In certain other embodiments, the disease is selected from the group consisting of hypertrophic cardiomyopathy, polycystic kidney disease and mucopolysaccharidosis. 
         [0023]    In certain embodiments of the invention, each of the oligonucleotide probes is detectably labeled, for example, with a fluorescent label, where the fluorescent label can be selected from the group consisting of ROX, VIC®, HEX, NED and FAMT™. 
         [0024]    In further embodiments, the assay plate comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 arrays. In certain other embodiments, the characteristics are identified using a single array, and/or the plurality of polymorphisms is simultaneously identified using one, two or three assay plates. 
         [0025]    In certain other embodiments, the method of the invention provides for a forward primer that is capable of hybridizing to a region within a nucleotide marker sequence that is about 30 to about 60 nucleotides upstream of the polymorphic site present within said nucleotide marker sequence. In further embodiments, the method of the invention provides for a reverse primer that is capable of hybridizing to a region within a nucleotide marker sequence that is about 30 to about 60 nucleotides downstream of the polymorphic site present within said nucleotide marker sequence. 
         [0026]    In certain embodiments, the simultaneous identification of said plurality of polymorphisms and determination of said characteristics is performed using a processor-based system. 
         [0027]    The invention further provides for a computer readable device having computer readable code embodied therein, said code embodying instructions for causing a processor-based system to identify a plurality of polymorphisms in a nucleic acid sample, comprising: instructions that cause a processor-based system to identifying a plurality of polymorphisms in a nucleic acid sample according to any one of claims  1 - 37  as originally presented; instructions that cause the processor-based system to hybridize said nucleic sample to said primer sequences and to said oligonucleotide probes; and instructions that cause the processor-based system to detect the presence of said plurality of polymorphisms in said nucleic acid sample. 
         [0028]    The invention also provides for an assay plate to be used in the method of the invention. Thus, the invention provides for an assay plate comprising a plurality of recesses, wherein each of said recesses contains a composition, wherein each of said compositions comprises: (a) a pair of forward and reverse primers; (b) a first oligonucleotide probe; (c) a second oligonucleotide probe; and (d) a nucleic acid sample isolated from an animal; wherein said first oligonucleotide probe is capable of detecting a first allele of a sequence said nucleotide marker sequence; wherein said second oligonucleotide probe is capable of detecting a second allele of said nucleotide marker sequence; wherein said nucleotide marker sequence is any one of the nucleotide marker sequences as set forth in Tables 1-11; wherein said nucleotide marker sequence correlates with at least one of the characteristics of an animal selected from the group consisting of: (i) parentage; (ii) identity; (iii) sex, (iv) genotype and (v) phenotype; wherein said assay plate is capable of simultaneously identifying a plurality of polymorphisms; and wherein said plurality of polymorphisms correlates with least two characteristics of said animal selected from the group consisting of: (i) parentage; (ii) identity; (iii) sex, (iv) genotype and (v) phenotype. 
         [0029]    The invention further provides for a composition comprising a plurality of nucleotide marker sequences, wherein each of said nucleotide marker sequences comprises a polymorphism, and wherein said plurality of nucleotide marker sequences correlates with at least two characteristics selected from the group consisting of: (i) parentage; (ii) identity; (iii) sex, (iv) genotype and (v) phenotype; wherein each of said nucleotide marker sequences is any one of the nucleotide marker sequences as set forth in Tables 1-11. 
         [0030]    The invention also provides for a method of identifying a plurality of nucleotide marker polymorphisms comprising (a) contacting a nucleic acid sample with the composition comprising a plurality of nucleotide marker sequences; (b) hybridizing said nucleic acid sample to a pair of forward and reverse primer sequences; (c) performing PCR amplification of said nucleic acid sample; (d) hybridizing said amplified nucleic acid sample obtained from step (c) to said plurality of nucleotide marker sequences in said composition; and (e) identifying said plurality of nucleotide marker sequences; wherein said plurality of nucleotide marker polymorphisms correlates with at least two characteristics selected from the group consisting of parentage, identity, genotype and phenotype. 
         [0031]    With regard to the methods above, the invention provides for a computer readable device having computer readable code embodied therein, said code embodying instructions for causing a processor-based system to identify at least two characteristics selected from the group consisting of parentage, identity and phenotype, comprising: instructions that cause a processor-based system to contact a nucleic acid sample with the composition comprising a plurality of nucleotide marker sequences; instructions that cause the processor-based system to hybridize said nucleic acid sample to said plurality of nucleotide marker sequences in said composition; and instructions that cause the processor-based system to detect oligonucleotide sequences within said nucleic sample that have hybridized to said plurality of nucleotide marker sequences; wherein said plurality of nucleotide marker sequences correlates with at least two characteristics selected from the group consisting of parentage, identity and phenotype. 
         [0032]    The invention also provides for a method of determining at least two characteristics of an animal selected from the group consisting of: parentage, identity and phenotype, comprising (a) contacting a nucleic acid sample with the composition comprising a plurality of nucleotide marker sequences; (b) hybridizing said nucleic acid sample to a pair of forward and reverse primer sequences; (c) performing PCR amplification of said nucleic acid sample; (d) hybridizing said amplified nucleic acid obtained from step (c) to said plurality of nucleotide marker sequences in said composition; and (e) identifying a plurality of nucleotide marker polymorphisms within said nucleic acid sample that have hybridized to said plurality of nucleotide marker sequences; wherein said plurality of nucleotide marker polymorphisms correlates with at least two characteristics selected from the group consisting of parentage, identity and phenotype. 
         [0033]    The invention further provides a computer database comprising the nucleotide marker sequences as set forth in Tables 1-11. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0034]      FIG. 1  provides an exemplary assay plate or panel upon which a plurality of samples or assays may be stored for processing in accordance with any of the methods of the present invention. The assay plate includes an array of recesses, which may be implemented as wells or through-holes. 
           [0035]      FIG. 2  provides an exemplary processor-based system which may be used to process nucleic acid samples. 
           [0036]      FIGS. 3A-J  provides a series of scatter plots depicting identity data generated by the present invention. In each plot, homozygous populations are provided in the upper left and lower right and heterozygous populations are provided in the upper right. Specifically  FIGS. 3A-J  provide examples of identity, forensic and parentage markers for various species.  FIGS. 3A-C  provide examples of identity, forensic and parentage markers for cats.  FIGS. 3  D-F provide examples of identity, forensic and parentage markers for dogs.  FIGS. 3G-I  provide examples of identity, forensic and parentage markers for horses.  FIGS. 3  J provides examples of identity, forensic and parentage markers for cattle. The chart below is an example of the assay name correlating with the genomic location in cats. 
           [0000]    
         
           
                 
               
                 
                 
                 
               
             
                 
                     
                 
                 
                   FIGS. 3 A-C 
                 
               
            
             
                 
                     
                   Cat Assay Na           
                   Cat Genomic Location 
                 
                 
                     
                     
                 
                 
                     
                   FC07 
                   B1: 156,143,186 
                 
                 
                     
                   FC22 
                   C1: 123,746,252 
                 
                 
                     
                   FC24 
                   A3: 14,410,638 
                 
                 
                     
                   FC25 
                   F1: 33,007,663 
                 
                 
                     
                   FC27 
                   E2: 35,480,527 
                 
                 
                     
                   FC44 
                   A3: 48,181,817 
                 
                 
                     
                   FC48 
                   B3: 149,673,110 
                 
                 
                     
                   FC52 
                   B2: 159,389,942 
                 
                 
                     
                   FC01 
                   Un: 51,831,052 
                 
                 
                     
                   FC09 
                   A2: 17,611,273 
                 
                 
                     
                   FC10 
                   B3: 107,303,663 
                 
                 
                     
                   FC17 
                   A1: 15,263,737 
                 
                 
                     
                     
                 
                 
                     
                               indicates data missing or illegible when filed 
                 
               
            
           
         
       
           [0037]      FIGS. 4A-D  provide a series of scatter plots depicting non-disease trait data generated by the present invention. This can include but is not limited to color, color patterns, hair length, or other physical characteristics. Data points positioned in the upper left include those homozygous for the first allele the lower right provides those homozygous for the second allele and data points in the upper right provide the heterozygous population.  FIG. 4A  includes scatter plots demonstrating the presence of polymorphisms associated with color or other physical characteristics in cats. Examples included are DILUT which is dilute coat color in cats, CHOC2 (brown) which is chocolate coat coloration in cats, BLK (black) which causes recessive black located in the agouti gene in cats and CINNAM which is cinnamon coat color in cats. Sequences are provided in Table 8 under the name of the marker for example; Cinnam is the assay name and is the CINNAMON sequence in Table 8 DILUT is MLPH DILUTION in Table 8  FIG. 4B  includes scatter plots demonstrating the presence of polymorphisms associated with color or other physical characteristics in dogs, Examples are TYRP1-MC1R-S41C which denotes one SNP responsible for brown coat color in dogs, DOG-MASK-MASK causes a dark coloration or facial mask on dogs, MC1R-Yello-Yell is responsible for red to yellow coloration in some breeds of dog, and AGOUTI_DOG-R96c is associated with black coloration and it located in the agouti gene in dogs. Sequences for these markers are in Table 6 under trait names.  FIG. 4C  includes scatter plots demonstrating the presence of polymorphisms associated with color or other physical characteristics in horses, Examples are HORSE-MC1R-RED which denotes one SNP responsible for red coat color in horses, TOBIANO-TOB causes a white pattern or painted appearance in horses, SILVERH-SILH is silver coloration in horses. E AGOUTI-10 is bay pattern in horses. Sequences are in Table 2 under a similar trait name.  FIG. 4D  includes scatter plots demonstrating the presence of polymorphisms associated with color or other physical characteristics in cattle, Examples are BLCK which is responsible for red or the lack of red (black) coat color in cattle. The sequence can be found in Table 11, as RED. ALBIN causes a lack of pigment or white animals with pink or blue eyes and pink skin. The sequence can be found in Table 11, as Albino. In  FIG. 4  scatter plots depict animals negative for the trait or disease in Red (VIC). 
           [0038]      FIG. 5  provides a series of scatter plots depicting of sex determination data generated by the present invention. Data is shown from 3 species cat, dog, and cattle. ZFXY2 is cats, ZFXY1 is cattle and zfxy1_CF-xy2 is dog. Vic (Red) color denotes females and Green color (heterozygotes) denotes male animals. In  FIG. 5  scatter plots depict animals negative for the trait or disease in Red (VIC). 
           [0039]      FIGS. 6A-C  provide a series of scatter plots depicting disease trait data generated by the present invention.  FIG. 6A  includes scatter plots demonstrating the presence of polymorphisms associated with diseases in cats, Examples include MPS 1 which is Mucopolysaccharidosis Type VI and MPSM which is Mucopolysaccharidosis Type VI Mild Form. BLDAB is B blood type in cats responsible for neonatal isoerythrolysis. Sequences are available by name in Tables 7-11,  FIG. 6A  also includes 1 scatter plot demonstrating the presence of polymorphisms associated with diseases in dogs as does  FIG. 6B . In  FIG. 6A  MDR1-MDR is Multi-drug resistance in cancer in dogs. In  FIG. 6B , SCID is severe combined immunodeficiency in dogs, VW GERM-VW1 is von Willibrand&#39;s Disease Type 2 in dogs and CYST_DOG-CYST is Cystinurea in dogs. Sequences can be found in Table 6 under disease names.  FIG. 6B  also includes 1 scatter plot demonstrating the presence of polymorphisms associated with diseases in horses as does  FIG. 6C . In  FIG. 6B , HORSE_JEB-JEB is Junctional Epidermolysis Bullosa (JEB) and is Sequence ID 62 in Table 2.  FIG. 6C , Examples include HYPP_NEW-HYP which is Hyperkalemic Periodic Paralysis in horses and is Sequence ID 64 in Table 2 and HORSE —  LWO-LWO which is Lethal White Overo in horses and is Sequence ID 60 in Table 2. In  FIG. 6  scatter plots depict animals negative for the trait or disease in Red (VIC). 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Definitions 
       [0040]    It is to be noted that the term “a” or “an” entity refers to one or more of that entity; for example. “a nucleotide marker,” is understood to represent one or more nucleotide markers. As such, the terms “a” (or “an”), “one or more,” and “at least one” can be used interchangeably herein. 
         [0041]    As used herein, “about” means within ten percent of a value. For example, “about 100” would mean a value between 90 and 110. 
         [0042]    The term “plurality” or “multiple” refers to two or more, between about 20 and about 10,000, between about 20 and about 5000, between about 20 and 200; 3000 or more, 200 or more and extending up to whole genome analysis, 100 or more preferably about 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 1000, 3000, or 9000; more preferably about 64, 128, 3072, 6344 or 9216. 
         [0043]    The term “nucleotide” or “polynucleotide” or “nucleic acid” is intended to encompass a singular nucleic acid as well as plural nucleic acids, and refers to an isolated nucleic acid molecule or construct, e.g., messenger RNA (mRNA) or plasmid DNA (pDNA). A poly nucleotide may comprise a conventional phosphodiester bond or a non-conventional bond (e.g., an amide bond, such as found in peptide nucleic acids (PNA)). The term “nucleic acid” refer to any one or more nucleic acid segments, e.g., DNA or RNA fragments, present in a polynucleotide. In other embodiments, a polynucleotide of the present invention is cDNA, genomic DNA, mitochondrial DNA (mtDNA), or RNA, for example, in the form of messenger RNA (mRNA). 
         [0044]    By “isolated” nucleic acid or nucleotide is intended a nucleic acid molecule, DNA or RNA, which has been removed from its native environment. For example, a recombinant nucleic acid corresponding to a nucleotide marker contained in a vector is considered isolated for the purposes of the present invention. Further examples of an isolated nucleic acid include recombinant polynucleotides maintained in heterologous host cells or purified (partially or substantially) polynucleotides in solution. Isolated RNA molecules include in vivo or in vitro RNA transcripts of polynucleotides of the present invention. Isolated polynucleotides or nucleic acids according to the present invention further include such molecules produced synthetically. In addition, polynucleotide or a nucleic acid may be or may include a regulatory element such as a promoter, ribosome binding site, or a transcription terminator. 
         [0045]    By “derived from” is intended an isolated nucleotide, a synthesized nucleotide (e.g. an automated synthesizer), or a nucleotide whose sequence has been obtained from a genomic database and subsequently isolated or synthesized. 
         [0046]    As used herein, a “coding region” is a portion of nucleic acid which consists of codons translated into amino acids. Although a “stop codon” (TAG, TGA, or TAA) is not translated into an amino acid, it may be considered to be part of a coding region, but any flanking sequences, for example promoters, ribosome binding sites, transcriptional terminators, introns, and the like, are not part of a coding region. Two or more coding regions can be present in a single polynucleotide construct, e.g., on a single vector, or in separate polynucleotide constructs, e.g., on separate (different) vectors. In addition, a vector, polynucleotide, or nucleic acid of the invention may encode heterologous coding regions, either fused or unfused to a nucleic acid. Heterologous coding regions include without limitation specialized elements or motifs, such as a secretory signal peptide or a heterologous functional domain. 
         [0047]    In certain embodiments, the polynucleotide or nucleic acid is DNA. In the case of DNA, a polynucleotide comprising a nucleic acid which encodes a polypeptide normally may include a promoter and/or other transcription or translation control elements operably associated with one or more coding regions. An operable association is when a coding region for a gene product, e.g., a polypeptide, is associated with one or more regulatory sequences in such a way as to place expression of the gene product under the influence or control of the regulatory sequence(s). Two DNA fragments (such as a polypeptide coding region and a promoter associated therewith) are “operably associated” if induction of promoter function results in the transcription of mRNA encoding the desired gene product and if the nature of the linkage between the two DNA fragments does not interfere with the ability of the expression regulatory sequences to direct the expression of the gene product or interfere with the ability of the DNA template to be transcribed. Thus, a promoter region would be operably associated with a nucleic acid encoding a polypeptide if the promoter was capable of effecting transcription of that nucleic acid. The promoter may be a cell-specific promoter that directs substantial transcription of the DNA only in predetermined cells. Other transcription control elements, besides a promoter, for example enhancers, operators, repressors, and transcription termination signals, can be operably associated with the polynucleotide to direct cell-specific transcription. Suitable promoters and other transcription control regions are disclosed herein. 
         [0048]    The “target oligonucleotide sequence” or “target nucleic acid” may be a portion of a gene, a regulatory sequence, genomic DNA, cDNA, and RNA (including mRNA and rRNA). Genomic DNA samples are usually amplified before being brought into contact with a nucleotide marker sequence. Genomic DNA can be obtained from any tissue source or circulating cells (other than pure red blood cells). For example, convenient sources of genomic DNA include whole blood, semen, saliva, tears, urine, fecal material, sweat, buccal cells, skin and hair. Amplification of genomic DNA containing a polymorphic site generates a single species of target oligonucleotide sequence if the individual animal from which the sample was obtained is homozygous at the polymorphic site, or two species of target molecules if the individual is heterozygous. RNA samples also are often subject to amplification. In this case, amplification is typically preceded by reverse transcription. Amplification of all expressed mRNA can be performed as described in, for example, WO 96/14839 and WO 97/01603 which are hereby incorporated by reference in their entirety. Amplification of an RNA sample from a diploid sample can generate two species of target molecules if the individual providing the sample is heterozygous at a polymorphic site occurring within the expressed RNA, or possibly more if the species of the RNA is subjected to alternative splicing. Amplification generally can be performed using the PCR methods known in the art. Nucleic acids in a target sample can be labeled in the course of amplification by inclusion of one or more labeled nucleotides in the amplification mixture. Labels also can be attached to amplification products after amplification (e.g., by end-labeling). The amplification product can be RNA or DNA, depending on the enzyme and substrates used in the amplification reaction. 
         [0049]    As used herein, the term “polymorphism” refers to an allelic variant that occurs in a population that can be a single nucleotide difference present at a locus, or can be an insertion or deletion of one, a few or many consecutive nucleotides, or can be an inversion. A single nucleotide polymorphism (SNP) is characterized by the predominance in a population of certain nucleotides at a particular locus in a genome, such as the horse, dog, cat, cattle, or human genome. Typically, less than all four nucleotides (i.e., adenosine, cytosine, guanosine or thymidine) will predominate at a particular locus. For example, a particular locus in a genome of a specific population may contain either an adenosine or guanosine at the polymorphic site and thus two of the four nucleotides predominate at this particular locus. However, polymorph one or two, three or four nucleotides. It will be recognized that, while the methods of the invention are exemplified primarily by the detection of SNPs, the disclosed methods or others known in the art similarly can be used to identify other types of poly morphisms, such as an insertion or a deletion, which typically involve more than one nucleotide. 
         [0050]    A “single nucleotide polymorphism” or “SNP” occurs at a polymorphic site occupied by a single nucleotide, which is the site of variation between allelic sequences. The site is usually preceded by and followed by highly conserved sequences of the allele (e.g., sequences that vary in less than 1/100 or 1/1000 members of the population). A single nucleotide polymorphism usually arises due to a substitution of one nucleotide for another at the polymorphic site. Single nucleotide polymorphisms can also arise from a deletion of a nucleotide or an insertion of a nucleotide relative to a reference allele. 
         [0051]    The terms “nucleotide marker” and “marker” are used herein interchangeably to refer to a nucleotide sequence having a single nucleotide polymorphism (SNP), insertion or deletion, where the SNP, insertion or deletion renders the marker suitable as a molecular identifier of particular animal(s), and where the molecular identifier correlates with parentage, identity and/or phenotype of particular animal(s). A polymorphic site within the nucleotide marker (e.g. the site of an SNP, insertion or deletion) is the locus at which divergence occurs. Preferred markers have at least two alleles (allele 1 and allele 2), each occurring at a frequency of greater than 1%, and more preferably greater than 10% or 20% of a selected population. 
         [0052]    An “oligonucleotide probe” is defined herein as a nucleic acid sequence about 10, 12, 15, 18, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 or 35 nucleotides in length that spans a region of a nucleotide marker containing a polymorphic site (e.g., an SNP, and insertion or deletion). The polymorphic site may be positioned about the center of the oligonucleotide probe, within about 5 nucleotides of the center of the oligonucleotide probe, within about 10 nucleotides of the center of the oligonucleotide probe and the like. Such an oligonucleotide probe can be used in polymerase chain reaction (PCR) for allele discrimination or identification of an allelic variation. An oligonucleotide probe can also be used for hybridization to a target oligonucleotide sequence. Hybridization may occur through the use of arrays of nucleotide probes. 
         [0053]    The term “allele discrimination” refers to the determination of whether a DNA fragment contains two of the same alleles (either two allele 1&#39;s or two allele 2&#39;s) or two different alleles (one allele 1 and one allele 2) within a given nucleotide marker sequence. To achieve allele discrimination, two oligonucleotide probes can be labeled with two spectrally distinct dyes each identifying either allele 1 or allele 2. Results can be analyzed by measuring the level of fluorescence of each dye. Results can be plotted for comparison, such as on a scatter plot. In particular, if the fluorescent value of the DNA sample is high for allele 1 and low for allele 2, then the sample is homozygote for allele 1. Similarly, if the fluorescent value of the DNA sample is high for allele 2 and low for allele, then the DNA sample is homozygote for allele 2. If the DNA sample generates intermediate values for both dyes, it is heterozygote for both alleles. 
         [0054]    A “first oligonucleotide probe” refers to an oligonucleotide probe that hybridizes to either allele 1 or allele 2. A “second oligonucleotide probe” refers to an oligonucleotide probe that hybridizes to allele 2 when the first oligonucleotide probe hybridizes to allele 1, or that hybridizes to allele 1 when the first oligonucleotide probe hybridizes to allele 2. 
         [0055]    The term “quencher” is a compound used in PCR experiments that absorbs the energy of the reporter dye in its excited state. The quencher can emit its own fluorescent signal or emit no fluorescent signal. 
         [0056]    The term “reference dye” is used in PCR experiments for normalization of the fluorescence signal of the reporter fluorophore. The reference dye fluoresces at a constant level during the reaction. Reference dyes include ROX, VIC®, HEX, NED and FAMT™. 
         [0057]    The term “reporter dye” or “reporter fluorophore” refers to the fluorescent dye used to monitor PCR product accumulation of an oligonucleotide target sequence. This can be attached to a probe (such as with TaqMan or Molecular Beacons) or free in solution. This is also known as a fluorophore. Examples of reporter dyes are ROX, VIC®, HEX, NED and FAM™. 
         [0058]    As used herein, the term “mutation” refers to a sequence variation in a gene, such as a single nucleotide difference, an insertion, a deletion, or an inversion, that is associated or believed to be associated with a phenotype. The term “gene” refers to a segment of the genome that codes for a functional product protein control region. Polymorphic nucleotide markers used in accordance with the present invention for determination of parentage, identity and/or phenotype in an animal may be located in coding or non-coding regions of the genome. 
         [0059]    As used herein, the term “correlates with” refers to having a causal, complementary, parallel, or reciprocal relationship, especially a structural, functional, or qualitative correspondence between two comparable entities. In the present invention, for example, the identification of particular polymorphic sites (e.g., those within nucleotide marker sequences of the invention) in a nucleic acid sample derived from an animal, may correspond to the substantial likelihood of a particular animal having a certain identity, phenotypic trait, parentage, or combination thereof. The correlation between the presence of particular SNPs and the substantial likelihood of a particular animal having a certain parentage, identity, and/or phenotype has been established or demonstrated. The term “correlates with” can also be used in reference to drawing a conclusion about the parentage, identity and/or phenotype of an animal using a process of analyzing individually or in combination, nucleotide occurrence(s) of one or more SNP(s), which can be part of one or more haplotypes, in a nucleic acid sample of the subject, and comparing the individual or combination of nucleotide occurrence(s) of the SNP(s) to known relationships of nucleotide occurrence(s) of the SNP(s) in other animals. As disclosed herein, the nucleotide occurrence(s) can be identified directly by examining nucleic acid molecules, or indirectly by examining a polypeptide encoded by a particular gene where the polymorphism is associated with an amino acid change in the encoded polypeptide. 
         [0060]    The term “animal,” as used herein refers to an individual animal providing a nucleic acid sample from which target oligonucleotides are obtained for the purpose of identifying parentage, identity and/or phenotype of that animal. Animals are identified according to known classes of scientific taxonomy, such as family, genus and/or species. Animals of the present invention are of families including but not limited to Equidae, Bovidae, Canidae, Felidae, Camelidae, Cervidae, and Suidae. In particular, animals of the present invention include but are not limited to the family and genera Bovidae  Bos  (cattle), Bovidae  Ovis  (sheep), Bovidae  Capra  (goat), Bovidae  Bison  (bison) Equidae  Equus  (horse, donkey, mule), Canidae  Canis  (dog), Felidae  Felis  (cat), Camelidae  Vicugna  (alpaca), Camelidae  Lama  (llama), Camelidae  Camelus  (camel), Cervidae  Cervus  (deer), Cervidae  Alces  (moose, elk), Cervidae  Axis  (deer), Cervidae  Muntiacus  (deer), Cervidae  Dama  (deer), Cervidae  rangifer  (reindeer, caribou) and Suidae  Sus  (pig). 
         [0061]    As used herein, “hybridization” refers to the binding, annealing, duplexing, or hybridizing of a first nucleic acid molecule preferentially to a particular second nucleotide molecule. The stability of a hybridization complex varies with sequence composition, length and external conditions. Hybridization methods include those that rely on the control of stringency in reaction conditions to destabilize some but not all hybridization complexes formed in a mixture. Using these methods, it is possible to distinguish complete complementarity from partial complementarity between probe and target sequences that form a hybridization complex. 
         [0062]    The term “specific hybridization” refers to the binding, duplexing, or hybridizing of a molecule only to a particular nucleotide sequence under stringent conditions when that sequence is present in a complex mixture (e.g., total cellular) DNA or RNA. Stringent conditions are conditions under which a target oligonucleotide sequence will hybridize to a nucleotide marker sequence, but to no other sequences. Stringent conditions are sequence-dependent and are different in different circumstances. Longer sequences hybridize specifically at higher temperatures. Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (T m ) for the specific sequence at a defined ionic strength and pH. The T m  is the temperature (under defined ionic strength, pH, and nucleic acid concentration) at which 50% of the nucleotide marker sequences complementary to target oligonucleotide sequences hybridize to the target sequence at equilibrium. (As the target oligonucleotide sequences are generally present in excess, at T m , 50% of the nucleotide markers are occupied at equilibrium). Typically, stringent conditions include a salt concentration of at least about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short probes (e.g., 10 to 50 nucleotides). Stringent conditions can also be achieved with the addition of destabilizing agents such as formamide or tetraalkyl ammonium salts. For example, conditions of 5×SSPE (750 mM NaCl, 50 mM Na Phosphate, 5 mM EDTA, pH 7.4) and a temperature of 25-30° C. are suitable for allele-specific nucleotide marker hybridizations. 
         [0063]    A perfectly matched nucleotide marker has a sequence perfectly complementary to a particular target oligonucleotide sequence. Such a nucleotide marker sequence is typically perfectly complementary to a portion (subsequence) of the target sequence. 
         [0064]    The term “hapolotype” refers to the genetic constitution of an individual chromosome. Haplotype may refer to only one locus or to an entire genome. In the case of diploid organisms, a genome-wide haplotype comprises one member of the pair of alleles for each locus (that is, half of a diploid genome). The term “haplotype” also refers to a set of single nucleotide polymorphisms (SNPs) on a single chromatid that are statistically associated. It is thought that these associations, and the identification of a few alleles of a haplotype block, can unambiguously identify all other polymorphic sites in its region. 
         [0065]    The term “assay plate” refers to panel upon which a plurality of samples or assays may be stored for processing in accordance with any of the techniques described below. The assay plate includes an array of recesses, which may be implemented as wells or through-holes. 
         [0066]    As used herein, universal polymorphism identification system is synonymous with universal genetic evaluation. 
       Polymorphic Nucleotide Markers 
       [0067]    The present invention is based on the utilization of known nucleotide marker sequences containing single nucleotide polymorphisms (SNPs), insertions and/or deletions and other mutations that can be used to determine parentage, breed, identity, sex, genotype and/or phenotype in an animal. Accordingly, provided herein is an assay plate comprising a plurality of compositions, wherein each composition is capable of identifying a polymorphism contained within a nucleotide marker sequence of the invention. The polymorphic nucleotide marker sequences of the invention each have an occurrence of a polymorphism, wherein the occurrence of the polymorphism correlates with parentage, identity, sex, genotype and/or phenotype, or breed determination associated with that animal. 
         [0068]    Single nucleotide polymorphisms (SNPs) are positions at which two alternative bases occur at appreciable frequency (&gt;1%) in a given population, and are the most common type of genetic variation. The site is usually preceded by and followed by highly conserved sequences of the allele (e.g., sequences that vary in less than 1/100) or 1/1000 members of the populations). A single nucleotide polymorphism usually arises due to substitution of one nucleotide for another at the polymorphic site. A transition is the replacement of one purine by another purine or one pyrimidine by another pyrimidine. A transversion is the replacement of a purine by a pyrimidine or vice versa. Single nucleotide polymorphisms can also arise from a deletion of a nucleotide or an insertion of a nucleotide relative to a reference allele. Though in most embodiments a single nucleotide polymorphism is detected, the present invention also encompasses the dection of the presence, absence or substitution of a short series of nucletides in sequential alignment. In some embodiments two nucleotides in direct sequenctial alignment are present, deleted or substituted. In other embodiments, three nucleotides in direct sequential alignment are present, deleted or substituted. In other embodiments four nucleotides in direct sequential alignment are present, deleted or substituted. In other embodiments, five nucleotides in direct sequential alignment are present, deleted or substituted. In other embodiments, six nucleotides in direct sequence alignment are present, deleted or substituted. 
         [0069]    Single nucleotide polymorphisms may be functional or non-functional. Functional polymorphisms affect gene regulation or protein sequence whereas non-functional polymorphisms do not. Depending on the site of the polymorphism and importance of the change, functional polymorphisms can also cause, or contribute to diseases. 
         [0070]    SNPs can occur at different locations of the gene and may affect its function. For instance, polymorphisms in promoter and enhancer regions can affect gene function by modulating transcription, particularly if they are situated at recognition sites for DNA binding proteins. Polymorphisms in the 5′ untranslated region of genes can affect the efficiency with which proteins are translated. Polymorphisms in the protein-coding region of genes can alter the amino acid sequence and thereby alter gene function. Polymorphisms in the 3′ untranslated region of gene can affect gene function by altering the secondary structure of RNA and efficiency of translation or by affecting motifs in the RNA that bind proteins which regulate RNA degradation. Polymorphisms within introns can affect gene function by affecting RNA splicing. 
         [0071]    A polymorphic site can also contain an insertion, or additional base pairs within a region of DNA on one allele. In addition, a polymorphic site can contain a deletion, generated by the removal of base pairs within a region of DNA on one allele. The present invention can simulataneously detect deletions, substitutions and additions. 
         [0072]    The term genotyping or genotype refers to the determination of the genetic information an individual animal carries at one or more positions in the genome. For example, genotyping may comprise the determination of which allele or alleles an individual carries for a single SNP or the determination of which allele or alleles an individual carries for a plurality of SNPs. In making this determination, the alleles can be discriminated (allele discrimination). For example, a particular nucleotide in a genome may be an A in some individuals and a C in other individuals. Those individuals who have an A at the position have the A allele and those who have a C have the C allele. In a diploid organism the individual will have two copies of the sequence containing the polymorphic position so the individual may have an A allele and a C allele or alternatively two copies of the A allele or two copies of the C allele. Each allele may be present at a different frequency in a given population, for example 30% of the chromosomes in a population may carry the A allele and 70% the C allele. The frequency of the A allele would be 30% and the frequency of the C allele would be 70% in that population. Those individuals who have two copies of the C allele are homozygous for the C allele and the genotype is CC, those individuals who have two copies of the A allele are homozygous for the A allele and the genotype is AA, and those individuals who have one copy of each allele are heterozygous and the genotype is AC. 
         [0073]    Using the teachings herein, genotyping can be accomplished by determination of polymorphic sites within a nucleic acid sample. The genotypic determination can then be correlated with the parentage, identity and/or phenotype of an individual animal. Therefore, the compositions of the present invention can be used to determine the parentage, identity and/or phenotype of an animal regardless of breed. For example, the compositions can be used to determine the parentage, sex, identity, genotype and/or phenotype of an individual animal of a particular breed of cattle including, but not limited to, Angus, Limousin, Brahman, Jersey, Chianina, Brown Swiss, Santa Gertrudis, Shorthorn, Guernsey, Maine-Anjou, Simmental, Hereford, Holstein. Gelbvieh, Charolais or Beefmaster cattle, or a particular breed of horse including, but not limited to American Saddlebred, Andalusian. Appaloosa, Arabian, Miniature Horse, Quarter Horse, Paint, Paso Fino, Thoroughbred. AkalTeke, Standardbred, Tennessee Walking Horse and Icelandic, or a particular breed of dog including, but not limited to Afghan Hound, Australian Cattle Dog, Australian Shepherd, Basenji, Basset Hound, Beagle, Belgian Tervuren, Bernese Mountain Dog, Borzoi, Chihuahua, Chinese Shar-Pei, Chinese Crested, Corgi, Labradoodle, Cocker Spaniel. Collies, Dachshund, Doberman Pinscher, German Shepherd Dog, German Shorthaired Pointer, Golden Retriever. Greyhound, Labrador Retriever, Maltese, Mastiff Miniature Schnauzer, Poodle, Pug, Rottweiler, Saluki, Samoyed, Shetland Sheepdog. Siberian Husky, St. Bernard, Whippet and Yorkshire Terrier. 
         [0074]    Since genomic DNA is double-stranded, each SNP can be defined in terms of either the plus strand or the minus strand. Thus, for every SNP, one strand will contain an immediately 5′-proximal invariant sequence and the other strand will contain an immediately 3′-distal invariant sequence. In the present invention, the invariant sequence spanning the SNP is between about 20 and about 35 nucleotides in length, and more preferably 30 nucleotides in length. 
         [0075]    For the identification of multiple genetic characteristics, the present invention provides for a panel comprising a plurality of assay compositions, wherein each assay composition is capable of identifying at least one of the nucleotide markers as set forth in Table 1 below. Table 1 lists the name of the marker (SNP ID), the chromosome from which the marker is derived (Chr), the position of the polymorphic site within the chromosome (Position), a nucleotide that occurs at the polymorphic site (genomic allele (G)), the alternate nucleotide that can occur at the same polymorphic site (alternate allele (A)), other SNPs that occur within 30 by of the genomic/alternate allele (O), percent repeat (P) (percent of sequence that is repeated bases), the discovery breed (the breed(s) in which the SNP was identified) and the discovery read (the sequencing read where the SNP was identified): 
         [0000]    
       
         
               
             
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 HORSE SNP PANEL SEQUENCES (SET #1) 
               
             
          
           
               
                 SNP ID 
                 Chr 
                 Position 
                 G 
                 A 
                 O 
                 P 
                 Discovery Breed 
                 Discovery Read 
               
               
                   
               
             
          
           
               
                 BIEC323 
                 chr1 
                 1585996 
                 C 
                 T 
                 0 
                 0 
                 Andalusian, Arabian 
                 S257P6129FJ20.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P69RP21.T0 
               
               
                 BIEC35895 
                 chr1 
                 86195760 
                 A 
                 G 
                 0 
                 0 
                 QuarterHorse, 
                 S256P6119RI2.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred, 
                 S261P6121RN11.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 AkalTeke 
                 S259P6122RG18.T0 
               
               
                 BIEC67750 
                 chr1 
                 156029252 
                 A 
                 G 
                 0 
                 0 
                 QuarterHorse, 
                 Twilight, S256P6104FI11.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P630FE3.T0 
               
               
                 BIEC372460 
                 chr2 
                 10491958 
                 G 
                 C 
                 0 
                 3 
                 QuarterHorse, 
                 S256P656FM18.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
                 S255P6118RD21.T0 
               
               
                 BIEC382016 
                 chr2 
                 27765519 
                 G 
                 A 
                 0 
                 11 
                 Andalusian, Arabian, 
                 Twilight, S257P633RL14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S255P6124FH24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P6101FL20.T0 
               
               
                 BIEC404000 
                 chr2 
                 68717792 
                 T 
                 G 
                 0 
                 0 
                 Icelandic, Arabian 
                 Twilight, S258P678FH6.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P6124FO9.T0 
               
               
                 BIEC645002 
                 chr3 
                 1175654 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, 
                 Twilight, S261P630RM15.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S256P673FD9.T0 
               
               
                 BIEC661467 
                 chr3 
                 47244981 
                 A 
                 G 
                 0 
                 0 
                 QuarterHorse, 
                 S256P633FA11.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
                 S255P6123FI16.T0 
               
               
                 BIEC717039 
                 chr4 
                 17776766 
                 A 
                 G 
                 0 
                 0 
                 Thoroughbred, 
                 S261P623FO1.T0, S260P6114RM21.T0 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
               
               
                 BIEC733312 
                 chr4 
                 63503371 
                 G 
                 A 
                 0 
                 0 
                 QuarterHorse, 
                 S256P673FA10.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian, Andalusian 
                 S255P653FC24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P61RP9.T0 
               
               
                 BIEC748249 
                 chr5 
                 2999858 
                 A 
                 G 
                 0 
                 0 
                 Icelandic, 
                 Twilight, S258P676RM14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse, 
                 S256P622RC15.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred 
                 S261P667FD1.T0 
               
               
                 BIEC754184 
                 chr5 
                 15457472 
                 A 
                 G 
                 0 
                 0 
                 QuarterHorse, 
                 S256P69FE6.T0, S255P6110RL6.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian, AkalTeke 
                 S259P623RA18.T0 
               
               
                 BIEC778319 
                 chr5 
                 69493593 
                 T 
                 C 
                 0 
                 0 
                 AkalTeke, 
                 Twilight, S259P611RI24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred 
                 S261P643FO17.T0 
               
               
                 BIEC797384 
                 chr6 
                 43616437 
                 T 
                 G 
                 0 
                 0 
                 Standardbred, 
                 Twilight, S260P692RE18.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Andalusian, Quarter 
                 S257P6104FE12.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P624FO14.T0 
               
               
                 BIEC810015 
                 chr6 
                 69737444 
                 G 
                 A 
                 0 
                 0 
                 QuarterHorse, 
                 Twilight, S256P682RD24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
                 S255P652FM2.T0 
               
               
                 BIEC823988 
                 chr7 
                 10927001 
                 C 
                 T 
                 0 
                 0 
                 Thoroughbred, 
                 Twilight, S261P6144FH15.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 AkalTeke, 
                 S259P6116RA17.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P610FI11.T0 
               
               
                 BIEC846563 
                 chr7 
                 65694972 
                 C 
                 G 
                 0 
                 21 
                 Thoroughbred, 
                 S261P635RN2.T0, S256P670RA7.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse, 
                 S255P648FJ16.T0 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
               
               
                 BIEC866619 
                 chr8 
                 10338538 
                 T 
                 C 
                 0 
                 0 
                 Icelandic, Arabian, 
                 S258P650FJ8.T0, S255P665FK13.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P666FN18.T0 
               
               
                 BIEC880212 
                 chr8 
                 35993310 
                 C 
                 T 
                 0 
                 0 
                 Arabian, Icelandic 
                 Twilight, S255P6115FI12.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P678FP17.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S258P6124FM7.T0 
               
               
                 BIEC903524 
                 chr9 
                 4109222 
                 C 
                 T 
                 0 
                 0 
                 QuarterHorse, 
                 Twilight, S256P625RM11.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Andalusian 
                 S257P640FF1.T0 
               
               
                 BIEC933800 
                 chr9 
                 62529880 
                 T 
                 A 
                 0 
                 0 
                 QuarterHorse, 
                 Twilight, S256P662RK10.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian, Andalusian 
                 S255P621FP4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P6114FB2.T0 
               
               
                 BIEC100227 
                 chr10 
                 18562230 
                 A 
                 C 
                 0 
                 0 
                 AkalTeke, 
                 Twilight, S259P616RP5.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S256P655FH20.T0 
               
               
                 BIEC119261 
                 chr10 
                 59078213 
                 C 
                 T 
                 0 
                 0 
                 QuarterHorse, 
                 Twilight, S256P669RC20.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P629RG15.T0 
               
               
                 BIEC123028 
                 chr11 
                 48708 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, 
                 Twilight, S261P633RF4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 AkalTeke 
                 S259P6129FA13.T0 
               
               
                 BIEC141078 
                 chr11 
                 37812203 
                 T 
                 C 
                 0 
                 0 
                 Arabian, 
                 Twilight, S255P6108FD2.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred 
                 S261P631RN14.T0 
               
               
                 BIEC159353 
                 chr12 
                 7954220 
                 T 
                 C 
                 0 
                 0 
                 Arabian, Andalusian, 
                 Twilight, S255P63RA17.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S257P6130RL4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P646FD3.T0 
               
               
                 BIEC167336 
                 chr12 
                 18561559 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, 
                 S261P6122RB7.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred, 
                 S260P6111RH8.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Andalusian 
                 S257P69FB1.T0 
               
               
                 BIEC170689 
                 chr13 
                 4859954 
                 A 
                 G 
                 0 
                 3 
                 QuarterHorse, 
                 Twilight, S256P630RP16.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
                 S255P64RA9.T0 
               
               
                 BIEC177534 
                 chr13 
                 13499460 
                 G 
                 A 
                 0 
                 0 
                 Icelandic, 
                 Twilight, S258P613FO9.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S256P663RG14.T0 
               
               
                 BIEC187185 
                 chr14 
                 10519408 
                 G 
                 A 
                 0 
                 19 
                 QuarterHorse, 
                 Twilight, S256P620RE10.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P671FF16.T1 
               
               
                 BIEC214463 
                 chr14 
                 84065438 
                 C 
                 G 
                 0 
                 0 
                 AkalTeke, 
                 Twilight, S259P652FE18.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse, 
                 S256P651FD8.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Icelandic 
                 S258P665FI14.T0 
               
               
                 BIEC220494 
                 chr15 
                 54151 
                 A 
                 G 
                 0 
                 0 
                 Arabian, Andalusian, 
                 Twilight, S255P694FO14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S257P655RG14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P68FJ9.T0 
               
               
                 BIEC252403 
                 chr15 
                 57437448 
                 A 
                 G 
                 0 
                 0 
                 Andalusian, Quarter 
                 Twilight, S257P638FE3.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P67RA6.T0 
               
               
                 BIEC270317 
                 chr16 
                 18502832 
                 A 
                 G 
                 0 
                 0 
                 Standardbred, 
                 Twilight, S260P671RN2.T1, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
                 S255P673FD16.T0 
               
               
                 BIEC304838 
                 chr16 
                 87373220 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, 
                 Twilight, S261P6144FF22.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
                 S255P641RD20.T0 
               
               
                 BIEC306934 
                 chr17 
                 5112116 
                 A 
                 G 
                 0 
                 0 
                 Arabian, 
                 S255P680FH18.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S256P6135RN8.T0 
               
               
                 BIEC323723 
                 chr17 
                 78516552 
                 T 
                 C 
                 0 
                 0 
                 Andalusian, 
                 Twilight, S257P651RH19.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P683RP22.T0 
               
               
                 BIEC338343 
                 chr18 
                 45058553 
                 A 
                 G 
                 0 
                 0 
                 AkalTeke, Arabian, 
                 Twilight, S259P619RP3.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Andalusian 
                 S255P682FI21.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P694RG13.T0 
               
               
                 BIEC347016 
                 chr19 
                 8846168 
                 T 
                 C 
                 0 
                 0 
                 Arabian, 
                 Twilight, S255P654RL1.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred 
                 S261P641RH13.T0 
               
               
                 BIEC450770 
                 chr20 
                 56244068 
                 T 
                 G 
                 0 
                 0 
                 QuarterHorse, 
                 Twilight, S256P6139FI18.T0 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred, 
                 S261P625RO17.T0 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Andalusian 
                 S257P682FK1.T0 
               
               
                 BIEC465101 
                 chr21 
                 27637059 
                 A 
                 T 
                 0 
                 0 
                 Thoroughbred, 
                 S261P649RK22.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian, 
                 S255P648FB16.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P629FO7.T0 
               
               
                 BIEC486760 
                 chr22 
                 16193666 
                 T 
                 C 
                 0 
                 0 
                 Arabian, 
                 Twilight, S255P665FA16.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred, 
                 S261P649RK17.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P630RD15.T0 
               
               
                 BIEC507792 
                 chr23 
                 6819375 
                 C 
                 T 
                 0 
                 0 
                 Arabian, 
                 S255P652FC10.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred, 
                 S261P612RL13.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P696FL5.T0 
               
               
                 BIEC521111 
                 chr24 
                 7335306 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, 
                 Twilight, S261P697FO18.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S256P694FL8.T0 
               
               
                 BIEC547263 
                 chr25 
                 6279709 
                 T 
                 C 
                 0 
                 15 
                 QuarterHorse, 
                 Twilight, S256P678RH13.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred 
                 S261P612RK8.T0 
               
               
                 BIEC574261 
                 chr26 
                 27538107 
                 C 
                 T 
                 0 
                 0 
                 Arabian, 
                 Twilight, S255P64RN2.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P615FP22.T0 
               
               
                 BIEC585067 
                 chr27 
                 7673552 
                 C 
                 T 
                 0 
                 0 
                 Arabian, AkalTeke, 
                 Twilight, S255P6102RK14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred 
                 S259P633RF19.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S261P63RB23.T0 
               
               
                 BIEC609174 
                 chr28 
                 7989217 
                 C 
                 T 
                 0 
                 0 
                 Icelandic, 
                 Twilight, S258P622FB13.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Andalusian, 
                 S257P6115RF14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred 
                 S260P631RE16.T0 
               
               
                 BIEC628735 
                 chr29 
                 1807945 
                 G 
                 C 
                 0 
                 0 
                 Icelandic, 
                 Twilight, S258P6108RC18.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Standardbred, 
                 S260P6127RA4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 QuarterHorse 
                 S256P631RE3.T0 
               
               
                 BIEC688595 
                 chr30 
                 4306752 
                 A 
                 G 
                 0 
                 0 
                 Arabian, AkalTeke 
                 Twilight, S255P6127FC14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S259P654FN1.T0 
               
               
                 BIEC697335 
                 chr31 
                 2733738 
                 C 
                 T 
                 0 
                 0 
                 QuarterHorse, 
                 Twilight, S256P640RP19.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Andalusian 
                 S257P67FB15.T0 
               
               
                 BIEC938831 
                 chrX 
                 4928692 
                 A 
                 G 
                 0 
                 0 
                 Thoroughbred, 
                 S261P664RM16.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Arabian 
                 S255P653FJ4.T0 
               
               
                 CREAM 
                 Chr21 
                   
                 G 
                 A 
               
               
                 SILVER 
                 Chr6 
                   
                 C 
                 T 
                   
                   
                 Icelandic, Rocky Mtn 
               
               
                 TOBIANO 
                 Chr3 
                   
                 C 
                 G 
               
               
                 SABINO 
                 Chr3 
                   
                 T 
                 A 
                   
                   
                 Tenessee Walker 
               
               
                 AGOUTI 
                 Chr22 
                   
                 + 
                 GAAAAGAAGCA 
               
               
                 MC1R 
                 Chr3 
                   
                 C 
                 T 
               
               
                 LWO 
                 Chr17 
                   
                 TC 
                 AG 
               
               
                 GBE1 
                 Chr26 
                   
                 C 
                 A 
                   
                   
                 Quarter Horse 
               
               
                 JEB 
                 Chr5 
                   
                 + 
                 C 
                   
                   
                 Belgian 
               
               
                 SCID 
                 Chr9 
                   
                 + 
                 TCTCA 
                   
                   
                 Arabian 
               
               
                 HYPP 
                 Chr11 
                   
                 C 
                 G 
                   
                   
                 Quarter Horse 
               
               
                   
               
             
          
         
       
     
         [0076]    The nucleic acid sequences of the markers as set forth above in Table 1 are provided in Table 2 below, where the position of the polymorphic site (e.g., the single nucleotide polymorphism (SNP), insertion and/or deletion) is bracketed and indicated in bold (e.g., [T/C] indicates that this position is polymorphic and that the nucleotide at this position is either a “T” or a “C”). Thus, allele 1 of this marker would contain a “T” at the position indicated and allele 2 of the marker would contain a “C” at the position indicated. The determination of a T or a C at this position is correlative of at least one characteristic, such as parentage, identity, sex or phenotype): 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                 HORSE SNP PANEL (SET #1) NUCLEOTIDE MARKER SEQUENCES 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 BIEC323 
                 GCCTTGTGACATCACAGCTGGATGTGTGTGGCCATGTTCAGAACTTGGTC 
                   
               
               
                 (SEQ ID 
                 CCAGGAACTGGTGGGCACTCGCTCACATGTGGGTCTCTGGCTCTACCTCC 
               
               
                 NO: 1) 
                 TGCCTGCTGGCCCAAACTTTGGGCCAGAGCCACACAAACTCCTTCTCTTT 
               
               
                   
                 AAACACCACTGCTTCCCTCCTCCTCGCTGATCTGTAGCTTTCCCCCGATT 
               
               
                   
                   [T/C] GGGATGTTCTTACTGCACATCCTGGGCATTTCTCGTCTACATCACCT 
               
               
                   
                 GGTTTAGCGCCGTGGCATGCTGGCTCACATGTGCCACCACAGCTGCATG 
               
               
                   
                 AGGGTTTCTCCAGGAGCAGGAGGGTTGGGCGAAGAGGCCAAGATTCCTC 
               
               
                   
                 GCTGAGCACTTGTCACATGGAGATTGCTGAGAAAATTCTGTAGATTTCA 
               
               
                   
                 AAGGAT 
               
               
                   
               
               
                 BIEC35895 
                 GGGTAGATTTAGAGATAAAGAGAGAGATGAGAGTCTAGGGTTNGATTTT 
               
               
                 (SEQ ID 
                 ATGGCCCTCGTAATATTATCCGCACTAGGAGTTGATATGAGCTCTGCTGA 
               
               
                 NO: 2) 
                 ATATGGCCTGGTCGTAAAGAGTGTGCTGGGAGGATGCTGGCACGTGTGC 
               
               
                   
                 TCAATGTCTACAGCCTTGAGGAAGCCTTGCACATCAGGCACCCCGAGTC 
               
               
                   
                 AAA [G/A] GGAGAGTGGTTCGTGGCGGGATCAACTTACGGATTGGAATCT 
               
               
                   
                 GGTGTCTTTGTAGATCGAGGCTATGAACTCTAGCTGGGCACCNCGACCA 
               
               
                   
                 CCTTCCCTCCTTGTCACAGGCAAAGGAGCCATGCCGCATCTCTGAAGAA 
               
               
                   
                 GTGCAGGGAAGATGCGACAGAAGGCGAAGGGACCCAAACACCACCAAG 
               
               
                   
                 AAGGCGCATTGA 
               
               
                   
               
               
                 BIEC67750 
                 GCTGCACATGGTTGAGTTATGAATCAAGCTTGTTCTCCCGATGCGGGAA 
               
               
                 (SEQ ID 
                 AATGGGCCGATGAATCCATTTCCATCACGACACTACCCATAAGTCATGG 
               
               
                 NO: 3) 
                 ATTGAAGGGCACTTTTCTTCCTTCTGGAATTTCCAATGACAAAGATTTCA 
               
               
                   
                 TTATCCAAAGCAATAATTTGTAACCAGAGCAATGCATGATTTCACTCTAC 
               
               
                   
                 CT [G/A] AATTAGGTCCATTGTGAGAGGGAGGCTGGGACAACCTCAGTGT 
               
               
                   
                 CTGGAGGGGCAGAAGAGGGTGAAATTGGACTCCTTCTTTGCTCTCGCCC 
               
               
                   
                 CTCCCCCATTCTTTCCCTTTTTCTCTCTGTGTGGGTGTTCACTTTCTTTGTT 
               
               
                   
                 CTCAGTCTCCTTTCTCTTGAGCCAACCCATCTCCTGGTGCTCTGCACTGG 
               
               
                   
                 CCATGA 
               
               
                   
               
               
                 BIEC372460 
                 CCAAAGTCCTAAACTCTAGCCTCCCGTTGGTTCTCACCCTCGCGTTTTAG 
               
               
                 (SEQ ID 
                 CGTTCTAATAGTGATCTTGAGANTCTTTGGCACGGAGCAAAGCTCCTCTT 
               
               
                 NO: 4) 
                 TGACCCAGTGCAGCGAGGGCCTCCACAACCTGCCCTACCCCTTTCGATTC 
               
               
                   
                 CCTTCCTCCCCTTAGCCCCACCTGGCTCAGGAAGAGTACAGAACGCATC 
               
               
                   
                 A [C/G] GGCGAGGAGGGGCTCCGAAAGTTACAAACCTGCGCTGCCCGCCA 
               
               
                   
                 CCTGCCCCTCCGAGCGCGCCGCGCGGCTTAAAGTGCCGCTGGGGATGCC 
               
               
                   
                 CCCCTTCCCCCCGTACCCCAACCCCGACCGTCACCTGGAGCCGGAAGCG 
               
               
                   
                 CTGCGCAGCCTTGTCCATGTTCTCCAGGGCCGCTTGCTCGCCGCTGTCGC 
               
               
                   
                 GCCCGGGC 
               
               
                   
               
               
                 BIEC382016 
                 GTAAAATAAGAGATGCACTATCTCTCTGATATTCTCTGCTCTGGACCTGA 
               
               
                 (SEQ ID 
                 GCTGCACCTCAGAAAGGGGCTCTTTCTAGCAGAGAGGGGAGTGAGGTTG 
               
               
                 NO: 5) 
                 CCATTTTGCTGCCACTCCCAAGAGGGCAGGCCCTGAGACTATTTCTGTCT 
               
               
                   
                 CTCCTCTCCTGATTCCCCACCCCTCCTGTCTAGACTGAGCTGAGTAACTG 
               
               
                   
                 T [A/G] TCTCCATGCTGGTGGCGGGGAACGACCGTGTGCAGACCATCATCA 
               
               
                   
                 CTCAGCTGGAGGACTCCTGTCGAGTGACAAAGGTGAGAGGAAGAATAG 
               
               
                   
                 CTCTGCCTGGGGCTTCGAAGCCTCCCAGTGTGGCCCGGTCTCTGGGGTGA 
               
               
                   
                 AGGCTCACCCATGGGATGTAACTAAAGGTTGGGGCCTGGAGCCTGGAGT 
               
               
                   
                 GTTCAGGC 
               
               
                   
               
               
                 BIEC404000 
                 ATATTAAAATTGTTGTCTACCCTCAGACCACACCAGGCACAGTATCCCA 
               
               
                 (SEQ ID 
                 AGGGCATGTGAATAGGAAGTGCTCAAGCCATAGGCATTGCCTTGATCCT 
               
               
                 NO: 6) 
                 TCCACAGAGCTGCTAATTAGCCTTGGGTCAGTCAGAAAACTGGTTACTC 
               
               
                   
                 AGCTTTGAAACTTCTAGGCAAATTTTGCTCTTAAGTATATAGCACGACAA 
               
               
                   
                 AAA [G/T] AAGTGATAAAGACCTTTTAACAACGTGTCTTCATTTTACTTAT 
               
               
                   
                 ACTCTTCCTATTGTCATCACCTCCTGAGGAAGCCTTTATAACCAAAACAC 
               
               
                   
                 ACACTTGATGAAGAGAACGTGAGCAGTCAAAAAAACACATATGTACAC 
               
               
                   
                 CGAAGACACAACTTAATTTTGGTTGGGTCCTCATGCAGCCAGGACAGAG 
               
               
                   
                 AAAACTTTTG 
               
               
                   
               
               
                 BIEC645002 
                 ATGTCTAGATTCCAATTTTATGAAACCTCAGATATGCAATATTATTTAAG 
               
               
                 (SEQ ID 
                 AGGTTAGAAGTGACTTTAATTTAAATTCAGATAAATAAGTTAATTTAGA 
               
               
                 NO: 7) 
                 GTTTTTGATATGATGAGAGTTTTTGTAACGCTGGATAAAGTTATGTTAGA 
               
               
                   
                 ACTTGACAAAACTCCTGAAAGGTTGATAACCAAAAGAAAGGTAGACTTG 
               
               
                   
                 TT [C/T] GTTGTCTTCTTGATATAGCTTCCATTTCTCTTTAGCTCCTTGACAG 
               
               
                   
                 TAAAGCAACTNTTTCTATTGTAATATCAGTACATGTTCTTCTTATTTCAAT 
               
               
                   
                 TTAGAAAAAAGCTGGTCTGTATCTGCCTGTAACATCTCTGAAGGGAGAA 
               
               
                   
                 ATCAGCTCACTTAACTTCCCTTCTCTTCCCCAAATGAATATTGTACCTTCC 
               
               
                   
                 ACT 
               
               
                   
               
               
                 BIEC661467 
                 TTGAAGTTTCGGTTGACTTATAGCTCTTTTTTGACCTTTGCTACTAATAGC 
               
               
                 (SEQ ID 
                 TGGCAGAGAATAAAACCAGACATGTCAATCACTGTCTAGATTTTATAAA 
               
               
                 NO: 8) 
                 CTATTGACTGTTTCTTGAAGGATGGTAATGCTTATTTTATTGGCCTCTTGT 
               
               
                   
                 ACCATAGAAGGGGATTTATAGAGTGCCAAAAAAATGAAAACCATGCCT 
               
               
                   
                 A [G/A] CTAGCATCATCCAGATCTGCTGGTAATAAAGTCACTGAAATTAAT 
               
               
                   
                 AACTATCAATTAAATATAAATGAGCTGAAACCACACCAAGGAGAGAATC 
               
               
                   
                 AACAGCATTTTAATTCCTAGATCTCTTTTGGCATTATCATATTTAATGCTC 
               
               
                   
                 TGTAAAGAGTTGAATTAACTAGTTTTCCTG 
               
               
                   
               
               
                 BIEC717039 
                 AAAACAGCTTCTTTGCAAATGAGAGCACAGCCGTGCTTCCCTGACTCCA 
               
               
                 (SEQ ID 
                 AATAGCCAAGTGAGAAGATGTCACCACAGGCTGCCCCTTCGTGACCTGG 
               
               
                 NO: 9) 
                 GCTAAGCCGAGTCCCCCAAATTTCCTCTGAACCTCTGGCTACAAAGAAA 
               
               
                   
                 TGTGCTTAGCTTGCTCAGGAGAGTTTGTCCAAACTCATGGAATTCATGGA 
               
               
                   
                 GGT [G/A] TTAAGTGTCAAGCTATTTTCTTCAGTTTCCCCTTTTACCCTCAC 
               
               
                   
                 ATGTCACCCCCTCTTAAAAGTTTTTTTAAAGTGAAATACAAAATTTATCC 
               
               
                   
                 AAAGAGAAATGAGATTTCCTGGATAAAGCATTTGTGTGATACTATTTCT 
               
               
                   
                 GAGTCTGTGTCTCTGAAAAAATGGCTGGGAAATCCCCTCGTCTCTACATT 
               
               
                   
                 TAAGCAT 
               
               
                   
               
               
                 BIEC733312 
                 GCTTTCCAAAGTGTTGGACAAGCTAAGTCCTATGCAATTATGCAATACTT 
               
               
                 (SEQ ID 
                 TTAAAAAAAACTTGAATTTTAAAATATGCTTGAAAAATAGATGCTGATC 
               
               
                 NO: 10) 
                 CAACAAGAACAGAGACTATCGATGAAAAGAATGTGTTCTCTGTGCACCT 
               
               
                   
                 AAGGGAAGCCAACACACAGGCAGCCTCATGTGGCCTGAAGCTTCAGGA 
               
               
                   
                 CTTT [A/G] GGAAGTTGCTTGCAGATGAATTTCTTTGAAATGAATAGCTCA 
               
               
                   
                 GGGCGGCATATGCCCTCCCTCTAGATTTGACTTCTGTGGTTTATGTATAA 
               
               
                   
                 GCTGGGGAAGACCTCAGAGTCTGACCTAGACTCACGTTATGTGCCTCTG 
               
               
                   
                 AAGTCTGGTGAAAGGCCAGACTTTAGGATTCCGCAGAGTTGAGAGTTGA 
               
               
                   
                 GTGAGGAACC 
               
               
                   
               
               
                 BIEC748249 
                 ATCAGTCCTTCAGGTTCTGGAAGCCCTCCATGCTGAGGCAATTTATGTTT 
               
               
                 (SEQ ID 
                 CTCTGCCTAGGGCACGGGGAACGCCTTCACCTCCACTCAGACCCTTCTGG 
               
               
                 NO: 11) 
                 ATTTCTCCCAGCCAAGGAAGTATGGAGCTCCAGAAGAATCTTACGAAAA 
               
               
                   
                 GTTTCTGAGCATAGGAGAGAGTACGTTTACCTTTAGGGCAGCGTTTCCTG 
               
               
                   
                 C [G/A] CGTCTGCTGCATCACCCCAGCCTAGCTTAACTACCTGAACACACG 
               
               
                   
                 GCTGGACTTGAGACCCTCTTAAGAATTCAAGTTTGTGGGGAATGGAAGC 
               
               
                   
                 TGGGAGGGAGTTGAGCAAGAAGGAAGGTTCCTATAGCTCTAGCAGCAC 
               
               
                   
                 GCCTAGTTCAGGGAGGAAGGACAGACGAGAGGGCATCATACTCACAAA 
               
               
                   
                 GAAAGTCTTC 
               
               
                   
               
               
                 BIEC754184 
                 CCCGCCATTGGCGGGGAGACCCGGCCTGGTGCTCGGGGCNCCCGGAGGG 
               
               
                 (SEQ ID 
                 TCCCAGAGAGAGACACGGAGGGCACGGAGGTCTNCCAGCTGCCGTTGCC 
               
               
                 NO: 12) 
                 CGCCCCNTGGGACTAGGGATTGCCGGAGATCTCGGAGAGGACCGGGGC 
               
               
                   
                 GGGGGAACTTTCAAAGGCGGGTCCGGCGACCCGGTGGGGAAGCGCCGG 
               
               
                   
                 AGCTCC [G/A] CCAGGCAGCAGACAAAACTCTCTGTCTGCCGGTAGCAGA 
               
               
                   
                 GGGGCCACGCTGAGNACTCAGGGCTCCCGGCAGAGGCCCGGANAGAAG 
               
               
                   
                 CCCAGAGGGCGGGGCGACCCCCAGCTGCCGTTGCCCGCCCCGNGGGACT 
               
               
                   
                 NGGGATTGCCGGAGATCTCGGAGAGGACCGGGGCGGGGGAACTTTCAA 
               
               
                   
                 AGGCCGGATCGGCGAC 
               
               
                   
               
               
                 BIEC778319 
                 TTCATAAATCCTGGAGTAATGGTGAAGCCTTTTTGGCTTAAATTTTACTT 
               
               
                 (SEQ ID 
                 GGGTTGTAAAATTAACCCCACTTTTATTGGGTAACTAATACCATAAACCT 
               
               
                 NO: 13) 
                 ATGGGGAGAAAGAAGTTTCTCGATGACCCAGTCTTGGATCTCGGAAACC 
               
               
                   
                 GAAGCCCTGAGACCAGAATGCCCCCCACGCCACCCCCTGCATTACAGAT 
               
               
                   
                 GA [C/T] GCTCTTCCACCCCCAGGCATTAGCAGAGCCCTGGATTTTTGAGG 
               
               
                   
                 TGTCATTATGAGAACACGTCTTCCTCCCCCACATTAAGCTCTCAAGGCCT 
               
               
                   
                 CAATTTATATCTCTTGGGAGGACAAAACCCTGGAATTACCCCAGATATA 
               
               
                   
                 AATCCCGACCTACTGGGAGTTCCCATATTTTATGTGAGGCTTAGGCTAAA 
               
               
                   
                 CTTCTTA 
               
               
                   
               
               
                 BIEC797384 
                 GTCATTTGCATCTCTAGCATTATTACAATTCTGAAAGTCATTTCAAATAA 
               
               
                 (SEQ ID 
                 GGTAAGTTTTAGAAGTGAAAGGAAACTTCTGGCATATTAGACATAAGTC 
               
               
                 NO: 14) 
                 AAGGACTCTTGTTTATGTCAAGCAATTCTACCACATATCTTTGTATGATT 
               
               
                   
                 AGGATAATTGTTTAGAATATTCTCCCAGGCACATTACTGTCAATTACTAA 
               
               
                   
                 A [G/T] ATTATATAGTCAGAGTCCCCACCTTGTATTCCGTTTGAATCACACT 
               
               
                   
                 GTTTTGCATTATTTTAAATGGCCACATTTTTATTTTTATCGAGGGGAACG 
               
               
                   
                 TAACTGCAAGGAATGTGGTTATCATGAGCTAATCTTACCCTTGGGGTATG 
               
               
                   
                 TGAGATATTTTCTAACTCTGAGATTGTGATTGCTTTCTGATTGCCATTCTG 
               
               
                   
                 CTC 
               
               
                   
               
               
                 BIEC810015 
                 CAGAGTGTTTTACTCCAAAGCGTAACTCNCATCACCCAGAGGGCTCCCT 
               
               
                 (SEQ ID 
                 GAATTCCACTTCTTCCTCTTGGAAGTCCTCCCACACGCGTCAGAAAAGAG 
               
               
                 NO: 15) 
                 CTCGTGGCTTCCTCTTCTTCACTCCCTGCCCCACCTGGGTCACCCACAGC 
               
               
                   
                 TACTCTCTTCCATGTTTATGACTCTCCATCGGCCCCAATCCCTGGAAATA 
               
               
                   
                 C [A/G] TTTGTTTTATAGCAAGAACACCTTGCTGCTTTCCTCCATCAGACGA 
               
               
                   
                 CTGCCCATCCCTCAGTGCTGGATATGTCACCCATACCAGTTTTTTGATTT 
               
               
                   
                 ATCTTTGAGAACAGTCTCTGCCAAGAATTCTTGAGTAGAATGTCATTCAA 
               
               
                   
                 CCATTGGCCATAACCATTCTCTCAGACAGCACATCTACAAAGGTCTCTCT 
               
               
                   
                 CGCA 
               
               
                   
               
               
                 BIEC823988 
                 AATAGGAATGCATTGCTTTCTGCAATCTGTCTTTGCTTGGAATCAGTAAC 
               
               
                 (SEQ ID 
                 AATATGTTCTGCAACTGTTATAAATTGAATGCATTTTCTTTATTGAGATA 
               
               
                 NO: 16) 
                 CATTNCTTTTTTTCATATAAATATTTAATTGGCCCTGAGAGAAAAGCTGT 
               
               
                   
                 TGGCATATTTCCTTCATTTGCTGTGGGGTTGGTGAATGATTCAGTCTTCA 
               
               
                   
                   [T/C] TGAATAGGGCAATTTCCTGGGGTTGACTACTGCGAGCTAAAAAGCT 
               
               
                   
                 CATCCATTTTCAGTCCTCCTTTAGAAAATGAAATAACTACAAATTTGTCC 
               
               
                   
                 TCTGTAAGCCATCAGAAAAAATGAAACAATTGACAAATCAGGTTCTAAG 
               
               
                   
                 AAGGAGAAACAGTTTATATTTTGTTTGTCTACTACTGTCATTTAAGTGTT 
               
               
                   
                 TACCT 
               
               
                   
               
               
                 BIEC846563 
                 CACTTCTCCTATGAATACTTCTCCAAACGGGATTCATGTCATCTCACTCC 
               
               
                 (SEQ ID 
                 ATTCTCATCCTGTTTTGGCCACAGTACAGTCACTGCCCAGTCCTTGAGCG 
               
               
                 NO: 17) 
                 AGCAAGACACAGCTGCTGTTTCATCAAGATGGTGTCTTGATACCTGAGTT 
               
               
                   
                 TCTCTGAGACCCTGGGTTCTGTGAGCTCTGCCCAGAGTCCAGAAGCCCTT 
               
               
                   
                   [G/C] AGTACAATCTCGCTATTAACCCTGGATCTCTCTTCACATCCTTTCCC 
               
               
                   
                 CCTTCTAGGCCACTTTTCCCTTCTCTCCATCCACTTGGATCCACAGCCTTT 
               
               
                   
                 AAGTCCGTCNCCAGCAGTcatcttcacttcaaggtctgacagcacctcaacttagtatgaccaggtggag 
               
               
                   
                 ctcttcattccactgtcaacccccaacttggt 
               
               
                   
               
               
                 BIEC866619 
                 GCGGGGAGAGAGCTAGCACACTGAGTCGGCTGCAGGCTCTGGCTGACG 
               
               
                 (SEQ ID 
                 GGCGAGGCTTACCTCTTGCCTAAGAAAGTGGCTTCCCCACATTTGAGACT 
               
               
                 NO: 18) 
                 TAAACTCATGCCTCAGAAACATCACCAGCAGCCCTTTTGCATGAATCTCA 
               
               
                   
                 GAACCTCCTTGGCAGCCGTAAACACACTTTACAAGTGGTCAACACTGGC 
               
               
                   
                 ATG [C/T] CAGAGGTCTGTGGGTTTCACACAGATTCCTTGGCGGGGCAAGC 
               
               
                   
                 TGGCTGGGGGACGGAAGCCCTCTGTGGCCTGACGCGCTGTCGTAGCCTT 
               
               
                   
                 GACCATGGCCTTTTTGTTTAAACAGACATTTCCAGGGAAGCCCTCAAAA 
               
               
                   
                 CATATTCGATTGGGAATGTCTCGTTCAGCAAAGCACATCTGATAGAGAG 
               
               
                   
                 AGATCTTGGT 
               
               
                   
               
               
                 BIEC880212 
                 TCAGAAGGAACCTCCCAGCCTCACCAACCACATATCTCCTTCAGTCACTG 
               
               
                 (SEQ ID 
                 AGCCTTCCAATTCATTTCCTTGCTGTTACTGTCTACTTTCTTCTATATATA 
               
               
                 NO: 19) 
                 GCACACATCCAAGGATGAGCATTTCTGAGATAGCCTTCTTTAGAATTGA 
               
               
                   
                 AAGACAGATCTCATTTAAAAGAGATGTAGTCTCTACTCCAAATTAGAAG 
               
               
                   
                 C [T/C] ATACAACTTCAACATGCTAGAAGTGTCTTAAAGAACTGATGCAAT 
               
               
                   
                 TTACATCAATGGCAACAACTTAGTGAACAACTTAAATCATATACAGTTT 
               
               
                   
                 ATAATTCAGATGTCAAAAAAACACTTAATATAACGTAACATCATAAAGG 
               
               
                   
                 NGATTGTGAAATATATGGACCTAAATTTTTGCTCTCTTTAAGAAACATTA 
               
               
                   
                 CAAAGTG 
               
               
                   
               
               
                 BIEC903524 
                 GTGACTGTGGCAGAACCTCATTACCAAAACTAAGGGTCCACCCTTACCT 
               
               
                 (SEQ ID 
                 TCCCCAAATGACATGTTATGCCTGAAGGTATCCAGACAATGTCANGTTG 
               
               
                 NO: 20) 
                 GTAGTAAACTTTTCTTTTNNTATGACCCCACTTCTGAAGGTAATCACTTC 
               
               
                   
                 TGATTTTTATTTTGCTCGTGACTTCACTAACCTGACTAAGACTGATTTTAT 
               
               
                   
                 G [T/C] CATCTGTTCCTGACTCTCAATATATTTTAACAAGTCAGAAACTAG 
               
               
                   
                 NGGGCTTAAGTCNGCATTTTCTGGACACAGATAAACNTTTTNCTTTTGTT 
               
               
                   
                 TTGTTTTTAANAATTCATTGAAAAGANNCAAGAAGGAAAACTTCCTCAA 
               
               
                   
                 GACAAGNGAGTTTGATTTGTTTTGTTTTTTACTAAGTTCCTCCAATATCA 
               
               
                   
                 AAGCTG 
               
               
                   
               
               
                 BIEC933800 
                 CCATAATCCTGTCCTTATTTCTCCACTTGGATGTGGCAGCACTGTAGCTG 
               
               
                 (SEQ ID 
                 CAGCATGGCCTTTTTTCTTCCAGAATCAGCTCTTCCTCCGGCAATCCCCG 
               
               
                 NO: 21) 
                 CCTCAGTGGATCATCCACATGAGCAAAAACCTGGAGTCATAATTGACTG 
               
               
                   
                 ACTCTCCTCCTTCACTCCCAATAACCACATTTCAGTTATTTTGCCTTTTAG 
               
               
                   
                   [A/T] TATTTCTCAAATCTGTTTTCTCTTTGTGCCCTGGTCCTTTTTCCCAGC 
               
               
                   
                 ACTTGTCTTTACTTATGTCCTTCCTTGTGGCTGAAATGCTTCTCTCACTTT 
               
               
                   
                 TGTTGTTATTGTTGTTAACACAGCTAGTGTGTTCTTGCTCATTCTTAAAGA 
               
               
                   
                 CTCNCTTCAGGCTGTATTGCCCTTTACTTCTATGCATGTGTCTTTCTTAA 
               
               
                   
               
               
                 BIEC100227 
                 TCACTTTTTCCATTTTGGGCTCATTGCTTGCCTAACTCAACTGCATCACTT 
               
               
                 (SEQ ID 
                 AGTATTCTTTTGGCTCAGTGGGAAAATAGTAAAATATCTAAGAACTTGA 
               
               
                 NO: 22) 
                 AGATCCAGAAATGCCTTCTTTCACTCTCGTTCTCTTTATCAAGTTACTTGG 
               
               
                   
                 AAACATTTTCTTATTTCAAATAAAGTGTAGGGTTCAAAGTGCTAGGAGA 
               
               
                   
                   [C/A] AAAAATCCATCAAGGATCCATCCCCCTAAAGCTCTTCTTGTCTCTCA 
               
               
                   
                 TGAAAACATGGCCCCACGTGGTGGGTTTAACCTGTGAGATTCAGGTCGG 
               
               
                   
                 AGTCTCCTGCTTGGGGACTTGCCCCTGCTGACNGTTTCTCCTTTGTCCCTT 
               
               
                   
                 AAAAATAATTTGGCTCCATATACAATTCTCCACAGACTCCTAATTCCTGG 
               
               
                   
                 AAA 
               
               
                   
               
               
                 BIEC119261 
                 CACAGAAAGGAAAGATACCCCCAAACATTTTCATGATGCGGCAGACTCT 
               
               
                 (SEQ ID 
                 ACAGAAAACTCGTGAATTAAGGCATTTCAGTAACAATAACTAATTCTAC 
               
               
                 NO: 23) 
                 CAACACCATTACTATAAAACCATTAACTAACTGACCAAAAAAATTAAGA 
               
               
                   
                 AAAAATGAGGAAATAGCAAAAGCCATAATTATGCTCCTGTAAGCAGACT 
               
               
                   
                 GAAA [T/C] TTTTGAAAAGTACACCATGTACGAACTACCAACATATAGAA 
               
               
                   
                 GTTTGAGCAATGGGCTGAGCACAAAGGAAAAGCTTACACTCACTCTTTG 
               
               
                   
                 AGGGTGTAGGGGTGTAGTGGGAAGGGAAGATGGTGATAAAAAAAACAG 
               
               
                   
                 TAGTCCCAATTCTGTATTGTGTTACCTACGCAATGTACCTACACAATGTC 
               
               
                   
                 ATCAATGACAA 
               
               
                   
               
               
                 BIEC123028 
                 AACATTCTAACTTGCTCCAATCAGACACAACGCCAAGGTTTCANGCAGG 
               
               
                 (SEQ ID 
                 TTAATGGAGAACCAAGAGATGGCACACAGCTCTGTGAGACGATGCCAG 
               
               
                 NO: 24) 
                 GGGACAGCCCAGCACAGAGCACAGGCCCTGGGATTCTCACTGGTCACGT 
               
               
                   
                 GGGAGTGGAGGACGCGGCATGAAGCAAGGGCATCTCCGCTCAGAAGGT 
               
               
                   
                 TCCCTG [C/T] GGAGCCCCACAGACAAGGCAGCGCGAGCAAGGCCCAAAG 
               
               
                   
                 AACAGGCTCCCCCGCCATGGGCTCCTCTCTGGCCCCAACGTGAGGACAG 
               
               
                   
                 CCATACTATGAAGACACAGCACTAAGGCAAAAAGCTCCTCATGTGGGAC 
               
               
                   
                 AGAAACCCACACCCCACCAAGATGGGTTCTGACTCCTCTATCGTTTTGGA 
               
               
                   
                 CTCCCTGAGAACC 
               
               
                   
               
               
                 BIEC141078 
                 GGGAACTGACCTACTCAGATCTGCCTCCAAGANGTCAGGAAGGAANTGC 
               
               
                 (SEQ ID 
                 AGACAGAGAAACNCTCACGCACAAACTGGAGAGTGGGGTTGGGCATGG 
               
               
                 NO: 25) 
                 CCGATCCCCGGAGCTGGTTGGCAGGTGGACACCAGCATCTAGCAGTAGC 
               
               
                   
                 CAGGTGGTCTGACTCCAGGACCAGAGCTGGGCTCTTCCGACAACATGAT 
               
               
                   
                 GCTCC [C/T] TGCATGGAAAGCCACGAGAGCTCTCCTCTCTTCTCTTCAGG 
               
               
                   
                 AAGCCAGTGGAAGAGGAGAACGGAGGATCGGAAGAGTTGTGCATGCAT 
               
               
                   
                 CTCCGGCAGTCTGGGGGTGGATGTGAGTCCAGGGGGGTAGGGCCGACTG 
               
               
                   
                 GGAAAATAGGAGCGAGGAGCCTGGTGGGGTGGCCCCCAGAATGGAGGT 
               
               
                   
                 GTCTGTGCCTGTGG 
               
               
                   
               
               
                 BIEC159353 
                 TCCAAACAGCCTGGCGGGCTTTCCCTGATATATCATCCTCACCCAGAGCC 
               
               
                 (SEQ ID 
                 GGTCTCGTGTCACTCCAGGACACCGAGGCAGGAAAAAGACTGACAGCCT 
               
               
                 NO: 26) 
                 GATGCGATATAATGTGAGTCCCCCCACCATGGGACACCCCCTGAGGTTC 
               
               
                   
                 TGTGGCCAGCCTGGCCCATGCCCAGGAGCTGTCACCCACCCAGCCTGAC 
               
               
                   
                 CTC [C/T] GGGCTCCCTCTCCCAACTGTGCCGAGGATCAAATGATAAGGAG 
               
               
                   
                 ACAAAAAGAAAACAGGGAGCTGGGGCCACACGTGAGATCGGCACCACT 
               
               
                   
                 TAGTCATCATCGCGCCCCCACCCCATGCTTACTCGTGACCAGGCCGATGC 
               
               
                   
                 CGGGGACGTGGTCTGCCAGCAGCTGGAGCAGGAAGAGGATCCTGGCCCT 
               
               
                   
                 GCAGGCGGGA 
               
               
                   
               
               
                 BIEC167336 
                 GCTCCCAGCCCGATCCCCAGCCAGCCTGGAGGACACTCTTCCCAGTGAT 
               
               
                 (SEQ ID 
                 CTCCCCCTGCTGCAGAGCTCACTATGGGCACAGTTCTGCACATGAGGAG 
               
               
                 NO: 27) 
                 GGGTCTCCACCAACATCTGCTGCCTGGATGGTGGCCNGCGACGTCCCCA 
               
               
                   
                 TCCCTGACATTTGCCCAGCACCCTGTTGGGCCAGAGCCTTTTCCACCCAT 
               
               
                   
                 GAC [C/T] TCCTTTGCTCCTTTTGTGAACTACCTTCCTGGTCTGACCCCCTC 
               
               
                   
                 ACATGCCCCCGGGCACCCTGCACGGCTCAGGACGGAGACCCGGGGTGG 
               
               
                   
                 GAAAGTCCAGGGTGCCTCGTGCTGGGCTGGGACCTGGGGTGGACTTGCC 
               
               
                   
                 CTCCCGAGGCTNGGGGCTCCATGCACANTTGCCCCACAGGCCTGTGTGC 
               
               
                   
                 CCCCAAGCTC 
               
               
                   
               
               
                 BIEC170689 
                 tagtccgtccctcACACACCCAAGATGGCATCCCTGTCTGGTCCAAAGCTGCAC 
               
               
                 (SEQ ID 
                 AATGTCCCACCCCTCTNGCTGCCCATTCCTGAGCATGAGGAGGTATTTCT 
               
               
                 NO: 28) 
                 GCTTCTCTGCCCCATTCCTGGGTTCTCTCTCTCATCTTCTGCTTGGGTTGG 
               
               
                   
                 CTGCACANACTCAGAGCTGCCTCAGGCCACTCCACAGTTTGGTCA [G/A] A 
               
               
                   
                 CATCCCATTGGAGTGAATCAGGATGCCTGGCGTGGTGCCTTCTACCTCCT 
               
               
                   
                 GCTGCCTGGACCACCATGTCCCCTTCCACCACTAGCATTTCATGAAGACA 
               
               
                   
                 CGTGTTTTCCAAGGCCTGTTCTGCTCCTTTAAAATGCAGTGTACATTTGA 
               
               
                   
                 AAGAGCGAGGGGCATTCTGGAGGCTAAGCTTGGGCATGTCTTTAGGGTA 
               
               
                   
               
               
                 BIEC177534 
                 TCAGATCTTTAACCAAGAATCGATTGATGGAGGCAGCATGGCCAGTGGG 
               
               
                 (SEQ ID 
                 ACAAAGAGGTAGTCAGGGCCACTGGTGTCACATGTGGCACTGAAATGGG 
               
               
                 NO: 29) 
                 TTCTTGGAGGTGCCAATAGCCAACCTTCCATCTGCCCAGTCTTTAAAGGG 
               
               
                   
                 AAATGAATGGGAGAATGGACTGCGTGGGGCATCAAGATCACTATTATTC 
               
               
                   
                 CCC [A/G] TTCCCTTTGCAATATTTTCAAACAGAGGATAGCATATCAAAAT 
               
               
                   
                 AAACACCAAGAACAAAGACATCTCTGATGTGCTTTTGTGCTGGCAGGAG 
               
               
                   
                 AGTGTTGTCTGCTCTCACAGATGGACTTAGCTTTGTCCAATGAAGAATTC 
               
               
                   
                 TGCAAGGGGTGTTATCACCTGAGCTTACCATAGACACCAGAATCTTGAA 
               
               
                   
                 TGAGATGGG 
               
               
                   
               
               
                 BIEC187185 
                 aagcaagaaagggaggaaggaaggaagcaaggaagggagggaggaaggaaggaagcaagcaaggaaggaagg 
               
               
                 (SEQ ID 
                 aaggaGAAACAGTAAAATAAAAAAACCAAAGGAAAAACTCAGGCAGAAG 
               
               
                 NO: 30) 
                 ATATGACAAATGGAAAAGATATGTTCTTCTTGTAGATCTGAAGTTCTCAC 
               
               
                   
                 TTGCAAGATGAGATAATACATCTTTGCTT [A/G] TTAATCTGGAATTATAA 
               
               
                   
                 TGTTTGAACCCTTGAAGTCCTCCAAGAAACTCAACCCTTAGAAAAACCC 
               
               
                   
                 ACAGCTGTCTCCTATAGGTTTTCAAATAATTGACAAGTATCTCTCAAACT 
               
               
                   
                 TGGAAGAATACCTTTAAGACTTCAGTACACACTCTCTGTCTTGACTAACT 
               
               
                   
                 GACAAAGCAGAGGAATTGAAACAGATACTTCACT 
               
               
                   
               
               
                 BIEC214463 
                 TGCATAGATTCAGAAGCCAGCTGGTGAGACAGCGTTATAAAGGAGGTAT 
               
               
                 (SEQ ID 
                 TTTAGAAAGANAAAAGTCTTGAGCAGAGGGTTTTGTTCACAAAAAGGGC 
               
               
                 NO: 31) 
                 AACAAACTACCTGTGCTAATAAGCTTATTCACNATAAAGTGACTGCTGT 
               
               
                   
                 GAGACTNTGTGAGGTCAGCTCATCACAGAAAGCTGTCACTCTACTGTAT 
               
               
                   
                 TACT [G/C] TAATAGACGTTTAAATACATGTTTCATGCCTACATCTCACTGT 
               
               
                   
                 TGTACGACTCGAACACATATAAACCTCAAATGTCAGGGCTACATTCAAT 
               
               
                   
                 TCAAGACGGTATGCTGTTAGCTCTCACAATCATAACTTGTATTCCCTGGG 
               
               
                   
                 AGGAATGTTCAGAAATGTTCCCCTTCCGATGTGAAGGCCTCTCTACCTCC 
               
               
                   
                 AGTCCAGT 
               
               
                   
               
               
                 BIEC220494 
                 GCGGGCCCTTGGCAGGAAGGACCTAGGGACTGTGCCGGGGCTTAGAGTG 
               
               
                 (SEQ ID 
                 CAGCCTTCAGTCCTGAGAGCTGATCAAGGAGAAGGGGCAGTTCCATGGC 
               
               
                 NO: 32) 
                 TCTGGAGAGGGTCTCCCCCTGCATACCCTGGCCACCTCGATCCACCGCTC 
               
               
                   
                 CAGGACCTTGGCCCTGCCCTGGGCCGTCATGTTTGGGTCCCCAAGGCAG 
               
               
                   
                 GAC [G/A] TCATGACACAATTTGCTACTCTGTTGAACTGCACCACTGTGGC 
               
               
                   
                 CCGGACGGTGGGTGCCACGTTCTCATGTCCAGGCTGGGTTCTTTTGCCCC 
               
               
                   
                 AGGTGGAGCTCAGATACTCAGAGGGCACGACGTTCTTGAACAATNCCTG 
               
               
                   
                 GTGAAGAGGGGGAGAGTCACTCAGCCCTGTCACAGCCCANCTCAGTGTC 
               
               
                   
                 CAGGCAGGA 
               
               
                   
               
               
                 BIEC252403 
                 AGAATGCCCCCTCTCTTTANGTAGAAACGGGCATGTGGGTGTTTCAGGC 
               
               
                 (SEQ ID 
                 CTCGCATTTAGATCATAGGAGATGGAAAGATCTCCCAGAGCCTGTTCCA 
               
               
                 NO: 33) 
                 CACTGCAGTTGTCCCCCAGTTCAATGACACTATTTTCTTAGGAGAAAACC 
               
               
                   
                 AAGTTATACCGCCACTTTGCCCTTTTGAAAACTGCGTCTGCTCATTTATTT 
               
               
                   
                 T [G/A] CTTGGTGCATATCTTAGACAGGCATTACCACAGTGGTTTGTAACC 
               
               
                   
                 TTCGATTTGTCCGTGTTCCCTCTTTGGCTTCAAAATGCCACACGACCCCC 
               
               
                   
                 TTTTGAGGTTGGAAAGAACCTCCTTTTCNCTATAATTTCAAGGGGAACTT 
               
               
                   
                 GCAAAGTATCACGATAATTGAAAAAAATCTGTATCATAATATCAGGATC 
               
               
                   
                 CAGGTT 
               
               
                   
               
               
                 BIEC270317 
                 TCGGCTCGAAACGTTTTCAAAGTAAACAAATGAGTTAGCAATTTACCAC 
               
               
                 (SEQ ID 
                 TTAGGATTCTCAAAGTGAGAGTTTATCCCACCAAAAGTAATTTTCCANCT 
               
               
                 NO: 34) 
                 CCTCCCCCTCAAGCCTATGCTGTCCTTTTGGCTACAGCATGGGCCAAAGG 
               
               
                   
                 TTGATAATACTTCTGTATACATTTAGCAAACCCAACCTCTACCAAACTAG 
               
               
                   
                 G [G/A] GAACGGCAAATGATACCGGTGGATAGAGACCCAGGGTGCTTTAA 
               
               
                   
                 CGTCAAATGCACAACTTGATGGCCGTCTCTCACCGTAGGACAGTGGAAC 
               
               
                   
                 AAGCAACTGCAGTGACTCAACATGAAGGGCAGGAATCTCCATAAAGTA 
               
               
                   
                 ATCTCCTGTTATCAGGAAATGTATTTATAACTATTTTGTAGATGGGTACC 
               
               
                   
                 CATGTCTCA 
               
               
                   
               
               
                 BIEC304838 
                 GAAGCAAACCGTGGGATAAGGGACCTGTCACTTTATAAGCAGCTCAGAC 
               
               
                 (SEQ ID 
                 TAACTGAAAGCGTGAAATACCTGTGGTTAGAGCTAATAACAAAATAACA 
               
               
                 NO: 35) 
                 GTTATACTCGTCACAAAAACATCTTACACAGGTGAAAACATGAGTAGTG 
               
               
                   
                 GAAAATGCAAGCTGCCATATGCAGGAGCAGAGCTGGCAACCCTGGAAG 
               
               
                   
                 ACTGT [C/T] GCTTGCCTCCCGAGGGTTCAGCAGAGGGCCTTGACGCCCCC 
               
               
                   
                 TCTCCGTAAGGAAAAGTCCAGGACACGGAGAGGGGAGGCAGTTTCTACC 
               
               
                   
                 AGAGAACCCATCTTACTCAACACCCTCCCCCCAAAGAGGATGGCAGCCC 
               
               
                   
                 CTGCGGCCTTGAAAACCCCAAAGCCTCAAAGCTCGGTGCCTCCCGCCTG 
               
               
                   
                 GCCCGAGAAAGG 
               
               
                   
               
               
                 BIEC306934 
                 TCAGGTCCTCCACATCCAGTTAAATTTATCCTGGAAGCAATAAAAATGTT 
               
               
                 (SEQ ID 
                 AAATATTACTTGGTTAGAGTTTCTCCTCCTTTATCTAGACGTAACTGTGT 
               
               
                 NO: 36) 
                 AGTGGGGGATAAATGGTTGTAATGCAGATATTCGAGAAGGTTCACTGAT 
               
               
                   
                 TCCTTCAGGCTACCTGGGGCCACTCATGACATGTTAACGAGTATTTACTG 
               
               
                   
                 T [G/A] TGTCTACTCTATGTCCCTATGCAATTTGACCCGATATTTTTAGTAT 
               
               
                   
                 TTCAGCTTGAGTTACCAAGTGATTCGGTAGGTATGTGGGAAAGTTTAAT 
               
               
                   
                 ATGTCTCCAATAACCAGTAACTTATTAAAAATGGATCTTCTCACATAGAA 
               
               
                   
                 CAGAGAGTTACTCTACCCAATCACCGAATAATTTCCAAAAATTACCCCA 
               
               
                   
                 GTTTAC 
               
               
                   
               
               
                 BIEC323723 
                 GACTTTTAATATTTGGATATGTGAAGATGTTTATAAATTGGTAGTATGGA 
               
               
                 (SEQ ID 
                 GAGTTCTGAATTNTATGCCCACGTTCTTGCCCAGGCAGAGTGACATTTCC 
               
               
                 NO: 37) 
                 CCTTCCTCCCGTCCAAACTAGGAGCAAAGGACTTTTTTAAGTAAATTATT 
               
               
                   
                 TTTAGATTTCCCAGAGATGCTTTTTAAAGAGCCGTTTGTTTTCGTAAACA 
               
               
                   
                   [C/T] GTTCGACGTGAGTTATTGTGAATTTTTGCTAATAGAGGCTGACAGTA 
               
               
                   
                 CACATAACACGAGCAGACAGCAAGGTACAGCCCCGGGCACCCGTCTTTG 
               
               
                   
                 GTGGCTGACAGGCTGAGGGATGAATGTTGAGAGCCCCGGACAGCCCCG 
               
               
                   
                 GGACCGACGTGTCAGGTCGGGCATGTGCCAGGCTCTCCCCTCTTTCTCGT 
               
               
                   
                 CTCCAG 
               
               
                   
               
               
                 BIEC338343 
                 ACAGTTGATGCTTAAATTGTCCTTGGACCCTGTAGCTGCTAGATTAGGAA 
               
               
                 (SEQ ID 
                 TTCTTCCTAATCTGTTCCCAACTTTATGTTTAGCACCAAAATATCTGTTTT 
               
               
                 NO: 38) 
                 ATTTCTTCTACTTCTCACAAATTGGAGAAATAAGAGTAATTCATAAATCT 
               
               
                   
                 TTCACCTCAATTATTCTCTTTTCTATAAATGACTAAAAAAATACATTCA [G/   
               
               
                   
                   A] GTAGGATTGTAAAAATCTATGACCAGACCCTTTGTTTTCAAGGTTATA 
               
               
                   
                 GAAACAACAAAAGCTTGAAATTTTACAAGGAAATTGTAATAATTTAGCT 
               
               
                   
                 CAGGTAAAGTGTAATTTTCTTCATGAAAAAGCAAATATTAATCTAAATA 
               
               
                   
                 GTTTTCAAGTGAGATATCAAAAAGAGACCTCATTAAGTAAATAAAATAC 
               
               
                   
                 CAATT 
               
               
                   
               
               
                 BIEC347016 
                 AACCCCCACAGCTGACTCGTCAGTCTGTCTTCAGCCACTAGTAACAAGC 
               
               
                 (SEQ ID 
                 CCAGTTCTATAGATGAGCCTCTCGAGGCCCAGAGCAGCTTGTTTATAGTC 
               
               
                 NO: 39) 
                 CTCTAGTTCGTAAGTGATGGAACCAGTCTTTACTTCTCCTACCTCCCTCA 
               
               
                   
                 AGCAGTGCACTGAGAGATAGAAACTAAGGATCAAAGAAACCACAATAC 
               
               
                   
                 TTC [C/T] TAGTGCTTCTTCTACAGATCTGAGAGTTTTTGAAAATGAGACC 
               
               
                   
                 AATTTGAGATAAAAGCCTTCAGCACTTGTTTCCAAAACTTAATTCATTCA 
               
               
                   
                 ACAAATATACCTGGCCCCCACTAGGAAATAACGGAGTCGGGGCAAGGC 
               
               
                   
                 AGTAAGGTCTCCACTCTTTTTGATGTACATGCTTGGTGGTGATGGTGGTG 
               
               
                   
                 GTGGGGTGA 
               
               
                   
               
               
                 BIEC450770 
                 ACGTATTGTGTTTTCTTTCTAAGTCTCCTACCAATTGTAAGTTCTCTCCCT 
               
               
                 (SEQ ID 
                 TGACCAAAATTGTGCAAATTTTCTTCTATATAGTCTTCTGTTTTTATTTTT 
               
               
                 NO: 40) 
                 ATATTTAGTGTTTACATTTTGTTCATATTAAAATTTCATTTACAGCATCTG 
               
               
                   
                 GATGTATCTTGCGCACAACAGCACCTAGGAGTGGATTAAACGTTTTT [G/   
               
               
                   
                   T] ATAATTTGGAAGCATTCTTTTGGTTTTCTATCACCTTCGGGCTATGATT 
               
               
                   
                 ATACAAACAGATTTTCACTGATCTGTGATTATACTTGTTAATAGAGCGCC 
               
               
                   
                 AGGCCACNGTCCTTCGAAGTGGCTGCACCCACCTGAAATCTATTCTTACA 
               
               
                   
                 CCTGCTCTAACACTGTCCTGAGTCCCCATTCTTGGGAATTCTGACCCATTA 
               
               
                   
               
               
                 BIEC465101 
                 CTGCCCCGGGAAAGCGGCTGGGGAACCAGTGTTCAGGGCCTATCCTCCC 
               
               
                 (SEQ ID 
                 ACAGATCCCACTCAGCATTCAGGCTATGCTGGTCAGTGGACTGGGGATT 
               
               
                 NO: 41) 
                 TTCCCTGGAGCCTTCTACAACACCAGGACTCCTGCTCCGTGATCTTCGGA 
               
               
                   
                 TGGAAATCCTTGTGCCCACTCTCACCCCTGCCCGCCCGGCCTGGCTTGTC 
               
               
                   
                 CC [T/A] AGAAGGATGATGTTCTATTCTTTCCCATCCCTGGAGTCCCTCTAG 
               
               
                   
                 TTGATTCAAAGAAGTGGGAATCATAGTAAAAAAGAAGAGAGACTCATCT 
               
               
                   
                 TCTGGTGGGTCTCGGTTCAGGATTTCTTACCTTCCTGATGTGTCTCCGTTT 
               
               
                   
                 GCAAGGTGGTCGTAAGGATGGATTCCTCTGGAGGGAGGGAAGGAACAG 
               
               
                   
                 GAGGAAG 
               
               
                   
               
               
                 BIEC486760 
                 CAAAGCTCCTTTTCTATGTCCAATGAATGGACAAATCCCTCAAACGATTA 
               
               
                 (SEQ ID 
                 AATAATTGCCCCAGGATCTAGGTGTAGCTTAATTCAGTTAGATTTAGAAT 
               
               
                 NO: 42) 
                 GTGAGGATTATAAGGAAGTCAAATCAAAATATGGAAAAAAACAAACAA 
               
               
                   
                 GACTTCTTTCTGAGCTAAGCTTCTCCAAAGGCTTTGGGAAAATCAACAG 
               
               
                   
                 AAC [C/T] AGTGATTTGATTTGAAAGTCCCTTCCTGTCTTAGGGTTGCACTT 
               
               
                   
                 TGAATGCCCCATACTTGTTCTTATGGACTGCTGACCAGAGTACCTCCACT 
               
               
                   
                 CCTTGATTTTCTCCTTAAGAACAAATTTCAGCATCCTAGAGAGAATGCTT 
               
               
                   
                 TCTAAATGGCAATTATCCTTAGGTTCCTGTTACCTAGGAATGTGTTTACC 
               
               
                   
                 AGTTGT 
               
               
                   
               
               
                 BIEC507792 
                 TTATACTTTGTATTATTTAAAGTACCTGATGCCATGCTACACAACTAGTG 
               
               
                 (SEQ ID 
                 TGTGTGCATCCGTTCATCTGACAGATATTTANTGACTCACCTNCCAGCTG 
               
               
                 NO: 43) 
                 ACTCCCTGGGAAGCCAGAGCAGTTTGCCAACTAATATGGTTAATTGGGA 
               
               
                   
                 TTTGATAAGTGCTATAAAGACACCAGAGATTCAGCAGTTTCTCCCAGCT 
               
               
                   
                 AT [T/C] GAACTTATTTTGGCTAATGGATATCACTCTCACTCCCATTTCAAT 
               
               
                   
                 CTTACAAAGGGATGCTAGAAGAGGATTGACCAGTCAGAAGGTGGAAAC 
               
               
                   
                 TTAATAAAAATTGNAAGTCAGAGACGGGAGGGAATGAAAGCAGCAGAG 
               
               
                   
                 GAAGAAGGAGGCAGAGACTGGACAGCCAGCAAGGCCTCAGTGCCCTGC 
               
               
                   
                 ACAGTTTAAGC 
               
               
                   
               
               
                 BIEC521111 
                 CAGTGTTTGCCACTCTTGCCAGCATTGAGAAGTCTCCCAGGCTGGTTAGG 
               
               
                 (SEQ ID 
                 GAAGCGGAGTGTGGCTGCTTGTCATGCTTTTCACCGGAGGGCAGTGTCT 
               
               
                 NO: 44) 
                 GATCCTCCTCGTGTCCCCACTCCTTCCCAGGTGACCCACATCACCACTCC 
               
               
                   
                 ACTTTATTCCTAAATCTTTGACTCGCTAAATCCTCCCTAAATCTCAGAAC 
               
               
                   
                 A [C/T] GGAACATGTTGAGGAGCTGGCATGCCCACAAACTCCTAAAAGGC 
               
               
                   
                 AACATGGGCCCCAGAAGGGCGCCNGGCAGGCAGGGAATCCTGATCTCT 
               
               
                   
                 AAGATAGGATTGTTAAACCCTGCAGACTCGGCTCCTTAGGAAATGCACT 
               
               
                   
                 GGTCTCAGAGAGAACNGAGACCCTGTCGGGAGCTCTTGGGATTTGTCTC 
               
               
                   
                 TCACTGTCCC 
               
               
                   
               
               
                 BIEC547263 
                 TTAGTTGTGGTGTATGTTAAGGCTAATTGCCTCCCCCACTAATTATTGAA 
               
               
                 (SEQ ID 
                 CAGCTGTCGATGCCATTTAATAATAGTCCACATTTTCCCAATTGATTTTA 
               
               
                 NO: 45) 
                 AATGATACCTTCATAATATATTCAATTTGACTTCTTGCTCCGATAACGTA 
               
               
                   
                 GCAGACTGAGGAATGTGGCCTCCTCCTGCTGAGCAACGTCAACCAAACT 
               
               
                   
                 T [C/T] GCAACAACAACAAAAGTAATTAATCTTGAAAGAAAGAAGGAATG 
               
               
                   
                 GGAGATGCCCAGGTGCTGGAAGATGGGAGGGAAAAACCAGAACCGGAA 
               
               
                   
                 GCTATTCCATGNCTGAGGACCCCAAAAAGGCAAGATCCTCAGTGAAGAg 
               
               
                   
                 agctcacagctgaaaatagagaccacaggctggaacgatctaccacgagggagagtcag 
               
               
                   
               
               
                 BIEC574261 
                 CAGATGAAGGAAATATACAAGCATTTGTAAAGCCCTTTTTAAAATAAAA 
               
               
                 (SEQ ID 
                 GAGAATTCAAAGACTTAAAAATATATCAACTTATTGTGACAAAACAATA 
               
               
                 NO: 46) 
                 TTCCTCTTGTCTTACCAGACTTCAAGAGAAAACTTCGAAGATGTCAGGA 
               
               
                   
                 NAACAGGTGTAGTGTTCTCTTTAAGATCGGCTCAGCCTCTGAATGTTGTA 
               
               
                   
                 AAC [T/C] TGCCAGCCTGACTTGCAAGAGTCAAGACGAGCACACAGGCGT 
               
               
                   
                 TTCTTACAGGCGGCACCAGCTCTCTGCCCAGAGGGAGCCAGCAAAATCC 
               
               
                   
                 CGGAAGCCTGTACACAGTTTTTCTCAGACCATGTATATGTTTAGAAGATA 
               
               
                   
                 GTACCNGGATGGCTCTAGGGAAAATTATTGGCTTCCATGTAAAACCCAA 
               
               
                   
                 AAGAAAGAAA 
               
               
                   
               
               
                 BIEC585067 
                 TTGCCACCAAAGGCAGACTCTGAATTTATGTTCATAATCCTGAGTCTGGG 
               
               
                 (SEQ ID 
                 TCACCAACGAATGTCTTTCTGGTGAGGCCTGAAATCTAAATGTTGGGAA 
               
               
                 NO: 47) 
                 TCCAACGGGTCTTGGCAGTGCATGGAAGGCTGTTTGCCAAATATCTGGA 
               
               
                   
                 TCTTATTTATTCCCTCCAGTCTCCCCAGAAAATGCTCTTGTTCATTTAAAT 
               
               
                   
                 A [T/C] GGACGTGACTACATTTGTTGGGGACCGTGTACTTTTTTCTTTAAAT 
               
               
                   
                 AGAAACGCCATGTGTGTGATGTTTTCTTTGAAAAGGAAAGCCCAGGAAT 
               
               
                   
                 TGTCTGCATCAGATTATAAAATGATCCCAGGGTCCATTCCTGGCTCTAAG 
               
               
                   
                 CAAGTTGAGTATACATCACCGCGTTTAATTCAGCAATATCATCAATGTCA 
               
               
                   
                 GTGCG 
               
               
                   
               
               
                 BIEC609174 
                 CTATCCTTGGGGGATTAGATGTTGAATATGTTTGGTATATAAATGTCATA 
               
               
                 (SEQ ID 
                 CTCAGATAAAATTTTTTGTTGGCAACACAAAAGAGCACAACATGCGACT 
               
               
                 NO: 48) 
                 AAAGCTAGAGAAATGTTCTGATTTCTATTAACTTTTATGTTCCTGTATAA 
               
               
                   
                 GCTAAAGCAGTTGAGACAATAGTAATTAGTCTTCCAATCTCCCATTCCTG 
               
               
                   
                 A [T/C] GTAATGGCTTGGCAGCTTTGAGGGTAGGGGAAGACTAATNAAGA 
               
               
                   
                 GTAGGTAGGTCAGTGTTCTGATGGACAGCAGCAATCGGCAGGACCATTA 
               
               
                   
                 ATACAGGACTGGAGTCCAGTTGGAAGTCTTATTATTATGGTGCTCATCTT 
               
               
                   
                 GCTTGTTCAGTTGCTGACCCACAACCCAATTTTACCCTTTACCTTTTCTTC 
               
               
                   
                 CGTGGA 
               
               
                   
               
               
                 BIEC628735 
                 TCTTCACTTCAAGAGCAGTTATCAGAACTTAGGGGTCTGAACTACAGAC 
               
               
                 (SEQ ID 
                 TTTTAGAGAACTAGATGGCAGATGATTCCCGTCACTGGGAATGNGGCAA 
               
               
                 NO: 49) 
                 TATCCCAATNACACAGCTGTTGCCACCAGCTGTGAGAACACAGACACTA 
               
               
                   
                 CACTGGATCAACTGGAAACACAAATGACCCCGGGTACAGACCACACGCT 
               
               
                   
                 AGGG [C/G] GCCACGACCATAACAAAAATACCTGCTGAGAGGAGGAGAA 
               
               
                   
                 CTGGCCAACAACGCTACCTCATGCAAAAGACACACTTCTGAGGAACACG 
               
               
                   
                 CCTGTGTCATCAAGATGATGTCCACTTGGGGCCATGCTACCTTCTGAGCC 
               
               
                   
                 ACTTCTGTTTCTCTGGCAGAATGTATCCAGCCTTCTGATTCAGGGCGTCA 
               
               
                   
                 GGATCACATCT 
               
               
                   
               
               
                 BIEC688595 
                 TTTGGACTAATTTAATGGCTTAATTTCAACATGATCACGATTAAATTGGA 
               
               
                 (SEQ ID 
                 TCTGCTGTTGAAAAAATAAAGAAAGAAAANACACTCACCCAGAACACTT 
               
               
                 NO: 50) 
                 CCAAAAGAGTGGATTTGAAAAGGAAAAACCAACCAACTCACCCCAGGG 
               
               
                   
                 CCCCAGCCCCCGCTAAGGTTCAGCCTCAGCGTTAAACACATTAACATGG 
               
               
                   
                 AGGA [G/A] AGGTGTGTGTTTCTTGCTCTGTAACAGGCACACGTTTCAAAC 
               
               
                   
                 ATAGGATTCAACATATGAGATTTGAAACATCTTTGGCATTTTGAAAAAA 
               
               
                   
                 TTTACAAACTAGAATCCCAGCTCGTTGGTTTTCTGAGTTGTTTAGGAAGT 
               
               
                   
                 CACTTAAAGGAAAATAAACATTCTCCNTGTGTTCGATGAATGCTACAGA 
               
               
                   
                 ACTTCCGTGC 
               
               
                   
               
               
                 BIEC697335 
                 CTTTACATTGCTTCTTGCTGATGATGAGTACTTTCATGATACCCTGTGCCT 
               
               
                 (SEQ ID 
                 GATCTACACATAATCAGAACCCTTCATTTCGTTCGGATGTGGTTCCATTT 
               
               
                 NO: 51) 
                 ATAAAATGTCAGGGGTGCAGACCAGCCCAGAACATCCTAAAACTTCCAG 
               
               
                   
                 AAGACAATAAAGTTTAGGCAACGGAGAAGTTGGTCGGGTGTTCCTCCCT 
               
               
                   
                 C [T/C] GTCCCTCGAGGTACAAGCTTCGGTTCTGCACGACTCAGCTTTCCA 
               
               
                   
                 AGGTGAGCTGCGTTGTCAGGTTGGAACACAGTTAGGTCTCAGACTGACT 
               
               
                   
                 TAGCGCTCACACCCACCCCTGTAGGCCTCTTACTTCTGTCCTCATGCCAC 
               
               
                   
                 CCTCCCATCCTCTTTCTTGGCAACAACGCCATGAAAAACGTGCAGATGG 
               
               
                   
                 GCTCCAT 
               
               
                   
               
               
                 BIEC938831 
                 TAGAGTGGTAGCATATTAAACAACACACAATATTCTATGGCTTGAATAA 
               
               
                 (SEQ ID 
                 TGGTGTTTATTTTCTTTAAATACGCTTAAATATGAAACAACAGACAGTAG 
               
               
                 NO: 52) 
                 TTCTGTAAACTCAAAACTAAAAGCTGGACGGTAATCAGAAAATCAGTGC 
               
               
                   
                 TGATCATACAAATCAAAATCTGCATAAGAGAATCAGAATAATTCACAAG 
               
               
                   
                 ACA [G/A] TTGCAATTAGCAAAGGACTCGGAGACTATGCGAATTGCCTCGT 
               
               
                   
                 CTGCTCTGAGTAATCAGTCACAGGGCACCTGTCATTCCATGATGATCAA 
               
               
                   
                 ACCTTTTTCCTTCACCAAAAAAAAAAAGGGTTATAGAGGTTTCCTCCTCT 
               
               
                   
                 CCATTCTTTGTTGTGCGGAGGCATCTTCAGTTAGAAGACATCTGTCAGTG 
               
               
                   
                 AACCAGGG 
               
               
                   
               
               
                 CREAM 
                 CTTTGATTGCTGACCGAAGGAAGAAGCTGACCTGGGCCATAACCATCAC 
               
               
                 (SEQ ID 
                 CATGATAGGTGTGGTTCTCTTT [G/A] ATTTTGCTGCTGACTTCATTGATGG 
               
               
                 NO: 53) 
                 GCCCATCAAAGCCTACTTATTTGATGTCTGCTCCCATCAGGACAAGGAG 
               
               
                   
                 AGGGGCCTCCACCACCACGCTCTCTTCACAGGTAGGGAATATTCCGGAA 
               
               
                   
                 AGTCTCTCCTTTAGCTCCCCAGACAGGGAGGTTCTTACACTGAAGCCATC 
               
               
                   
                 CAGTGTCTCTGCATGTCAAAGTTTTTGAATGAATGGATCAGCTGATGGA 
               
               
                   
                 ATGCTCTCATCACGCGGGCTCAGTCTCCCAGTGCATTTCTCTAAATAAAG 
               
               
                   
                 TCAACTTGTGACCAGGCTGAAGGGTTTTGCAAAGGAAGTTTACGTAAGA 
               
               
                   
                 GCTTTCTGAAGAACTATTTGTGGAGACATTTGCAAGTGAAATGAAAAGA 
               
               
                   
                 GGCATGGTGTACTTTTGGGGTGATTTTTTCTTTTTAAATCAGCAATGTGTT 
               
               
                   
                 TTTTTTAAGACAGCAATGTAGCATCGGTATTATTTAGGAGCTTGTTAAAA 
               
               
                   
                 ATGCAGTTCCATTGGGCCGGCCCAGTGGCGCAGTGGTTAAGTGCATACC 
               
               
                   
                 GTGTTGCTTCGGTGGCCCCAGGGTTACCAGCCTGGATC 
               
               
                   
               
               
                 SILVER 
                 CAGGTGAGGGCCCCACCATCCAGCGTACACCCCCTTATCCCTTATTACC 
               
               
                 (SEQ ID 
                 ACCACTCACTCTTCCTCAAGGGGAGAAGGAACCACCACTCCCTGTGAGG 
               
               
                 NO: 54) 
                 AAGCATGGTGTACAGGAAGGAGCCCAGACTTGGAAGTTAAACAGGCCT 
               
               
                   
                 GGCTTGCAGTCTTGCTGGTGAGACCTTGGAGGAAGTAGCCTAACCTTTCT 
               
               
                   
                 GAGCCTCTGAAAAGTAGGAAAATTAATACCTGCCCTGTGGGGGATGTTG 
               
               
                   
                 TCAGGATTAGAGACAATGTGAGTAAAGCTGGTTCTGAGGCAAGAGTGTA 
               
               
                   
                 ATAAAGGATCATATTGATGATTGTTATTAATAAGATAAAAAGTGGAGGA 
               
               
                   
                 GGTTGGCTGAACTGAGTTCTTCACCTGTAAGAGGGGCAGATCCCCAGGC 
               
               
                   
                 CTGGAATGCCAACGTCCTCAAAGCAGGGAAGCTTGTAGAGTGAGAGGG 
               
               
                   
                 GAATGGACAGAGGTTACCATATAAACAAGAGAAATGAACCCTGTTTGTG 
               
               
                   
                 AGGAGAAGAGGAGGCAGCTAGGATCAAGGCCAAGTAAACCTGGGATGT 
               
               
                   
                 GGGTGTGTCCTCTTCTTTGGAGAAGCACAGACAGGCTGCCCTTGTCCATT 
               
               
                   
                 GCTTACCAGTTTCCTTCTTCTTCTCCCAAATCAGG [C/T] GCAGACTTATGA 
               
               
                   
                 AGCAGGGCTCAGCTCTCCCCCTTCCCCAGCTGCCACACGGTAGAACCCA 
               
               
                   
                 CTGGCTGCGTCTGCCCTGGGTCTTCCGCTCTTCCCCCTTTGGTGAGAGCA 
               
               
                   
                 GCCCCCTCCTCAGTGGGCAGCAGGTCTGA 
               
               
                   
               
               
                 TOBIANO 
                 CAAGCGCTCATTTAACGGAACGAGAAGCCCTAATGTCTGAACTCAAAGT 
               
               
                 (SEQ ID 
                 CTTGAGTTACCTTGGTAATCACATGAATATTGTGAATCTTCTCGGAGCAT 
               
               
                 NO: 55) 
                 GCACCGTTGGAGGTAAAGCCGTGACCCGCTTGCATTTTATCACCTGTCGA 
               
               
                   
                 ATTATCAGAAGGGGGAGATTTTGATATGATTTTGACAATGCTTGATTATA 
               
               
                   
                 AGCTCCTTGCAAGATTTTTACCCAAGTTGTTGTTACCTCTTGCTAGAGTC 
               
               
                   
                 CCCCCTGCAAGAGTTATTGTTGTTAAGAGTGTATATTTTAGTTTTCTCATC 
               
               
                   
                 GTGGGGTGGGATAGTCCATGACATACCCTAGTTACTATCACGTATGTTGC 
               
               
                   
                 ATCCAAAGCATTGACCTCTTGAATATCTCGAGAATGTCTCATCTTGTGCC 
               
               
                   
                 CAAAGCTTTTCTTTTTATCTTGCAAGCTTTGTTTTGCTGGATCATATTTAG 
               
               
                   
                 TCACTAAAGGGTTAATATTCATTTGCATATCATAATTAAAAATAGGCCTT 
               
               
                   
                 TAAGCCTATCAGCTCACAAATATACACCAAATGAAGCAAACACCTCACT 
               
               
                   
                 CCTGTAAAAAATAAATTTCCAATTCTAAAGCATTAGTCAAGCCTCCCATC 
               
               
                   
                 AAGGATTTCTGTGGTGTTTCCAGAAAGCATTTTGGTCTTAAAGAAACAA 
               
               
                   
                 AGATTAGTAAAACGAGCTGTTCCTTGAGAACTGGAGAGATCCATGGTCA 
               
               
                   
                 CGTTGACAGCTTTATATAATTTCTTAAAGCAGACCCCATACCTTTTTGCC 
               
               
                   
                 TCACCACGTCATGACTCATTCGTGAGAAATTTCCGCC [C/G] GAGTTAATT 
               
               
                   
                 AGTTGCTTGTTACCTTCAGAGCTGCAGTTTTAGGCATTCACAACACCAAA 
               
               
                   
                 AAACATTTTCGCCTAGTAGTGCTCAGAACGCAGGTGACGCCACCTCATTT 
               
               
                   
                 CAGGTTGTCACCCGCTTTCCAGTCTCGTCTCAACGAGCTGGGCTGTTCCG 
               
               
                   
                 TGTGGTTGGTTTTCCTCTTGTGCCGCAGTTTGCGCTCTCATCGTTTCCGTA 
               
               
                   
                 ATAACGTTAACCGAGAGGCGTGATGCCCCACTGTAAACTGAAGGAAATC 
               
               
                   
                 AAAGGATTTATGTAGGACGGTTGATAGTAGCGTAAGTAACTCCTGTCTG 
               
               
                   
                 TAAAACCAGCAGTCACACCTGGCCCCTAATCTCTCAGAAATTCAGGTAA 
               
               
                   
                 AAAGGGGCTTGCTCATTTAGTTTTACTCCAAATAATGCCGGGTTTTGATA 
               
               
                   
                 AGCAATGTTAATAGAGAGGATTGTATTGGAACTAAGTAGGCTCATCCAT 
               
               
                   
                 TGAACCTGAATATTAATGACTCTGATCACCCTTGGGTATTTTAATGGGAG 
               
               
                   
                 GCAAGAATTGTCTATATGTCTCACCTCTTTCTACCCTTTTCCTATATGCTC 
               
               
                   
                 GTAGGGCCCACCCTGGTCATTACGGAATATTGTTGCTATGGTGATCTTCT 
               
               
                   
                 GAATTTTTTGAGAAGAAAACGTGATTCATTTATTTGCTCAAAGCAGGAA 
               
               
                   
                 GATCACGCAGAAGCAGCA 
               
               
                   
               
               
                 SABINO 
                 ACGAGCTGGCCCTAGACCTGGAAGACCTGCTCAGCTTTTCTTACCAGGT 
               
               
                 (SEQ ID 
                 GGCAAAGGGCATGGCGTTCCTTGCCTCAAAGAACGTAAGTGGGAAGAGT 
               
               
                 NO: 56) 
                 CCTTTTTTTTTTCCTTAATCGTGGAGCATTTTAGAGCCCTAGTTAGAATGC 
               
               
                   
                 AGAGTGTCATTTTGAAGTGTGGTAACCAAAAGCACAGGAAATTTAGTTT 
               
               
                   
                 CTTCATGTTCCAACTGCTGTCTCTTTGGAATTCCTGTTCTCATTTATAAGC 
               
               
                   
                 TTTAATGTGTAAGCCTGTCTAAATGAGCTTTCTATGAATATATTTTTGTAT 
               
               
                   
                 GCAATGAATTCATGTAAAACTTTTGGCTTTTAGGATATAGGAGCTGCTCT 
               
               
                   
                 GAGAAAATAGAGAAATAATTATTTTATCAGCAAAAGGAGCAGGTACCTC 
               
               
                   
                 ATGTAGTTGCAGTGCTTGGTGAAGCATATACTTGAGTCTTATTAAAGTTA 
               
               
                   
                 GACCCCAAATATTGCGTGTGGGTTTGTGTAGTGTAGGGGAAGAACCAAT 
               
               
                   
                 CAGGATAATAAACATTTGGGAAAAAGACGGGGGGGAGAGAGAATGATG 
               
               
                   
                 GAACCATAACATGGAACATGGTCCCTGGATAGGAGAGAGGAGTTCCCTA 
               
               
                   
                 GGACATGGGACTAGCAGAATAGAATAAGATTACAGATTCTGCCCTTAAG 
               
               
                   
                 TGTCGTTGGTGACATTTCCAAACAATTACCAAACTAAAAGAGGATATAG 
               
               
                   
                 GATGGCTGAAATAGCCTCTTCCCTGTGTCCTTGGGAGATGTCAAATTGAA 
               
               
                   
                 GTTGCAAAGACATTTTAGAAACTCTGTAAAAAGACAGTGAAAGAGAAG 
               
               
                   
                 CATGCAAAATGAGTCTCAGTTTAAAAAATATGATACAACTATTTATAAT 
               
               
                   
                 GTATTTTCCTGTGAATGAAAGCCGTCCTAAGAAGAAGAAAAGCATTTAT 
               
               
                   
                 TGGAGTTGGTTTTGAAAGTGATTATAGTAATTAAGGTCCTAACGATGAG 
               
               
                   
                 AAACACAAGTTTTGAACATCATTCAGAGCATAATTTAGATATTTATTTTT 
               
               
                   
                 GGTGCACTGAATAGTTTAAATGTAAAGCAAAAAGTATTGGATTGGTAGA 
               
               
                   
                 ACATAAGCAGCATTCTAGCATTAAACATAGTTTCACTCTTTAAAAGTTTA 
               
               
                   
                 AAATAAATTTAAATGGCTTTCTTTTCTCCCCC [T/A] CTCTCCTAATAGTGT 
               
               
                   
                 ATTCATAGGGACTTGGCAGCCAGAAATATCCTCCTTACTCATGGTCGAAT 
               
               
                   
                 CACAAAGATTTGTGATTTTGGTCTAGCCAGAGACATCAAGAATGATTCT 
               
               
                   
                 AATTATGTGGTCAAAGGAAATGTGAGTACTCACTCTCTGCTTGACAGTCC 
               
               
                   
                 AGTGAAGGATTTTAGTTTCACATTTTTATAATAAGTGTTTTTTATGATTTT 
               
               
                   
                 CGTAATGCAAATGCTCCCTTTGAGATAGCATGCATTTTAGCAGTCAAATT 
               
               
                   
                 AAGTGTACTTCAGCAAAATTTGTGTGGTATTGCTGAACCTTACTACAACT 
               
               
                   
                 AACAT 
               
               
                   
               
               
                 AGOUTI 
                 CCTCCCAATTCTCTGCAGTTCATGGGGTAAGGGGCGGTGGGGGAAGAGC 
               
               
                 (SEQ ID 
                 AAGGGGGAAAAGACCAGAAACATCTGGCTTTGCTCCTTTTGTCTCTCTTT 
               
               
                 NO: 57) 
                 GAAGCATTGAACAAGAAATCCAAAAAGATCAGCA [GAAAAGAAGCA/*]   
               
               
                   
                 GAAAAGAAGAAGAGATCTTCCAAGGTAGGCCTTGGACTTCTCATTGTAG 
               
               
                   
                 GGGTGGGACCAGACTTAAAAGGGGAGGACCCTGACCCTCAAGCTCTGGC 
               
               
                   
                 TAGGAACTAAATGAAGGATTTTTCAGGCCTACATGAACAAAAGAAGCTG 
               
               
                   
                 AAAGCTACCAAAAGGCTTCCTGGCCTGGAGCCCTGAACCAGACCCCACA 
               
               
                   
                 GAAGCTCAGGGAGCTGATGT 
               
               
                   
               
               
                 MC1R 
                 CACCCTCCCAGCCACCCCCTACCTCGGGCTGACCACCAACCAGACGGAG 
               
               
                 (SEQ ID 
                 CCCCCGTGCCTGGAAGTGTCCATTCCTGATGGGCTCTTCCTCAGCCTGGG 
               
               
                 NO: 58) 
                 GCTGGTGAGCCTAGTGGAAAATGTACTGGTGGTGACTGCCATCGCCAAG 
               
               
                   
                 AACCGCAACCTGCACTCACCCATGTACTACTTCATCTGCTGCCTGGCCGT 
               
               
                   
                 GT [C/T] CGACCTGCTGGTGAGCATGAGCAACGTGCTGGAGATGGCAATCT 
               
               
                   
                 TGCTGCTGCTGGAGGCCGGAGTCCTGGCCACCCAGGCCTCGGTGTTGCA 
               
               
                   
                 GCAGCTGGACAACATCATTGATGTGCTCATCTGCGGCTCCATGGTGTCCA 
               
               
                   
                 GCCTCTGCTTCCTGGGCAGCATTGCCGTAGACCGCTACATCTCCATCTTC 
               
               
                   
                 TATGCGCTGCGGTACCACAGCATCATGATGCTGCCCCGTGTGTGGCGTG 
               
               
                   
                 CCATCGTGGCCATCTGGGTGGTTAGTGTCCTCTCTAGCACCCTCTTCATC 
               
               
                   
                 GCTTACTACAACCACACGGCTGTCCTGCTCTGTCTCGTCACCTTCTTTGT 
               
               
                   
                 GGCCATGCTGGTGCTCATGGCAGTGCTGTACGTGCACATGCTCGCCAGG 
               
               
                   
                 GCGTGCCAGCACGCCCGGGGCATCGCCCGGCTCCACAAGAGGCAGCACC 
               
               
                   
                 CCATCCACCAGGGCTTTGGCCTCAAGGGTGCCGCCACCCTCACCATCCTG 
               
               
                   
                 CTGGGCGTTTTCTTCCTCTGCTGGGGCCCCTTTTTCCTGCACCTCTCACTC 
               
               
                   
                 CTTATCCTCTGCCCTCAACACCCCACCTGCGGCTGTGTCTTCAAGAACTT 
               
               
                   
                 CAAGCTCTTCCTCACCCTCATCCTGTGCAGCGCCATCGTCGACCCCCTCA 
               
               
                   
                 TCTATGCCTTCCGCAGCCAGGAACTTCGAAAGACG 
               
               
                   
               
               
                 LWO 
                 GCGACGCACCCTCCCTCCTCCCCCGTGCGAAAGAACCATCGAGATCAAG 
               
               
                 (SEQ ID 
                 GAGACTTTCAAGTACATCAACACAGTAGTGTCCTGCCTAGTGTTCGTGCT 
               
               
                 NO: 60) 
                 GGGCATCA [TC/AG] GGAAACTCCACACTGCTGAGAATCATTTACAAGAA 
               
               
                   
                 CAAGTGCATGCGGAACGGCCCTAATATCTTGATCGCCAGCCTGGCTCTG 
               
               
                   
                 GGAGACTTGCTGCACATCATCATTGACATCCCCATCAATGTCTACAA 
               
               
                   
               
               
                 GBE1 
                 ACGTGCCCGACCTGGGCCGCCTTCTGGAGGTCGACCCGTA [C/A] CTGAA 
               
               
                 (SEQ ID 
                 GCCCTACGCCCCGGACTTCCAGCGCA 
               
               
                 NO: 61) 
               
               
                   
               
               
                 JEB 
                 TGTTACTCAGGGGATGAGAACCCTGACATCCCTGAGTGTGCTGACTGCC 
               
               
                 (SEQ ID 
                 CCATTGGTTTCTACAACGATCCACAAGA [*/C] CCCCGCAGCTGCAAGCCG 
               
               
                 NO: 62) 
                 TGCCCCTGTCGCAATGGGTTCAGCTGCTCCGTGATGCCTGAGACAGAGG 
               
               
                   
                 AGGTGGTGTGCAATAACTGCCCCCAG 
               
               
                   
               
               
                 SCID 
                 TAGGAGCTCACTTTATAAGTTGGTCTTGTCATTGAGCTGTGGATATAGTC 
               
               
                 (SEQ ID 
                 ATTCTCTAATATTATTTTTAGGTAATTTATCA [TCTCA/*] AATTCCCCTTA 
               
               
                 NO: 63) 
                 AGAGACTTCTAAAAACCTGGACAAACAGATATCCGGATGCTAAAATGGA 
               
               
                   
                 CCCAATGAACATCTGGGATGACATCATCACAAATCGATGTTTCTTTCTCA 
               
               
                   
                 GCAAAATAGAAGAAAAACTGACTATTCCTCCAGATGATCATAGTATGAA 
               
               
                   
                 CACAGATGGAGATGAAGATTCCAGTGACAGAATGAAAGTGCA 
               
               
                   
               
               
                 HYPP 
                 GGGGAGTGTGTGCTCAAGATGTTCGCCCTGCGCCAAAACTACTTCACCG 
               
               
                 (SEQ ID 
                 TTGGCTGGAACATCTT [C/G] GACTTCGTGGTTGTCATCCTGTCCATTGTG 
               
               
                 NO: 64) 
               
               
                   
               
             
          
         
       
     
         [0077]    In further embodiments, the present invention provides a panel comprising a plurality of assay compositions, wherein each assay composition is capable of identifying at least one of the nucleotide markers as set forth in Table 1 above, and in the alternative, or in addition, is capable of identifying at least one of the nucleotide markers as set forth in Table 3 below. Table 3 also lists the name of the marker (SNP ID), the chromosome from which the marker is derived (Chr), the position of the polymorphic site within the chromosome (Position), a nucleotide that occurs at the polymorphic site (genomic allele (G)), the alternate nucleotide that can occur at the same polymorphic site (alternate allele (A)), other SNPs that occur within 30 by of the genomic/alternate allele (O), percent repeat (P), the discovery breed and the discovery read. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 HORSE SNP PANEL SEQUENCES (SET #2) 
               
             
          
           
               
                 SNP ID 
                 Chr 
                 Position 
                 G 
                 A 
                 O 
                 P 
                 Discovery Breed 
                 Discovery Read 
               
               
                   
               
             
          
           
               
                 BIEC20186 
                 chr1 
                 43890382 
                 T 
                 C 
                 0 
                 0 
                 Andalusian, Thorough 
                 Twilight, S257P677RC21.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P69RD10.T0 
               
               
                 BIEC41954 
                 chr1 
                 97804750 
                 T 
                 G 
                 0 
                 0 
                 Thoroughbred, Quarter 
                 Twilight, S261P666RO24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P6135FG2.T0 
               
               
                 BIEC51268 
                 chr1 
                 120359811 
                 G 
                 C 
                 0 
                 0 
                 Thoroughbred, Standard 
                 Twilight, S261P61RG20.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S260P642FI21.T0 
               
               
                 BIEC367927 
                 chr2 
                 1285181 
                 C 
                 T 
                 0 
                 10 
                 AkalTeke, Thorough 
                 Twilight, S259P6108RA4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P6125FN13.T0 
               
               
                 BIEC392749 
                 chr2 
                 46413743 
                 A 
                 G 
                 0 
                 0 
                 Arabian, Thoroughbred 
                 Twilight, S255P698FP19.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S261P614FG10.T0 
               
               
                 BIEC654812 
                 chr3 
                 30798767 
                 C 
                 G 
                 0 
                 0 
                 Standardbred, Quarter 
                 S260P65FD9.T0, S256P618FP14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse, Icelandic 
                 S258P675RK1.T0 
               
               
                 BIEC683021 
                 chr3 
                 100303903 
                 G 
                 A 
                 0 
                 0 
                 Arabian, Andalusian 
                 S255P6100RJ24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P662FE7.T0 
               
               
                 BIEC674509 
                 chr3 
                 82645698 
                 T 
                 C 
                 0 
                 0 
                 QuarterHorse, Thorough 
                 Twilight, S256P688RE3.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P669RD15.T0 
               
               
                 BIEC724885 
                 chr4 
                 39520660 
                 T 
                 C 
                 0 
                 0 
                 AkalTeke, QuarterHorse 
                 Twilight, S259P623FE3.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P627RF19.T0 
               
               
                 BIEC744445 
                 chr4 
                 95126218 
                 G 
                 T 
                 0 
                 0 
                 AkalTeke, Standardbred 
                 Twilight, S259P661RO24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S260P653RJ11.T0 
               
               
                 BIEC745623 
                 chr4 
                 96486556 
                 G 
                 C 
                 0 
                 0 
                 Arabian, QuarterHorse 
                 Twilight, S255P6102RL20.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P674FB16.T0 
               
               
                 BIEC751023 
                 chr5 
                 7672131 
                 G 
                 A 
                 0 
                 0 
                 Standardbred, Arabian 
                 Twilight, S260P628RO8.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P6130FM9.T0 
               
               
                 BIEC758316 
                 chr5 
                 29037866 
                 C 
                 G 
                 0 
                 0 
                 Arabian, Standardbred 
                 Twilight, S255P6113RE17.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S260P648FF20.T0 
               
               
                 BIEC784849 
                 chr6 
                 7074858 
                 T 
                 A 
                 0 
                 14 
                 Andalusian, Icelandic 
                 Twilight, S257P615FD15.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S258P699RK7.T0 
               
               
                 BIEC787485 
                 chr6 
                 12786580 
                 A 
                 G 
                 0 
                 0 
                 Arabian, Standardbred 
                 Twilight, S255P698RC13.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S260P686FF24.T0 
               
               
                 BIEC818096 
                 chr6 
                 81075556 
                 A 
                 C 
                 0 
                 0 
                 Arabian, Thoroughbred 
                 Twilight, S255P6125FC8.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S261P696FA19.T0 
               
               
                 BIEC818829 
                 chr7 
                 626858 
                 G 
                 C 
                 0 
                 0 
                 Standardbred, Andalusian 
                 Twilight, S260P6127FP10.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P6107FN7.T0 
               
               
                 BIEC837622 
                 chr7 
                 32398895 
                 A 
                 G 
                 0 
                 0 
                 Thoroughbred, Icelandic 
                 Twilight, S261P63FC21.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S258P6142FA24.T0 
               
               
                 BIEC855571 
                 chr7 
                 81268410 
                 A 
                 C 
                 0 
                 0 
                 Icelandic, AkalTeke 
                 Twilight, S258P612RC12.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S259P635FJ23.T0 
               
               
                 BIEC859610 
                 chr8 
                 868737 
                 T 
                 C 
                 0 
                 0 
                 QuarterHorse, Andalusian 
                 Twilight, S256P6118RH14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P621RJ15.T0 
               
               
                 BIEC870924 
                 chr8 
                 16053999 
                 A 
                 G 
                 0 
                 11 
                 Andalusian, Quarter 
                 Twilight, S257P633FI11.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P628FA18.T0 
               
               
                 BIEC888973 
                 chr8 
                 53031026 
                 G 
                 A 
                 0 
                 0 
                 Arabian, Standardbred 
                 Twilight, S255P659FM8.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S260P625RF12.T0 
               
               
                 BIEC921711 
                 chr9 
                 38627022 
                 G 
                 A 
                 0 
                 18 
                 QuarterHorse, Thorough 
                 Twilight, S256P631RJ5.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P644RM12.T0 
               
               
                 BIEC930198 
                 chr9 
                 56464461 
                 C 
                 T 
                 0 
                 0 
                 Arabian, Standardbred 
                 Twilight, S255P645RO24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S260P631RM5.T0 
               
               
                 BIEC111585 
                 chr10 
                 44275930 
                 G 
                 A 
                 0 
                 16 
                 Standardbred, Thorough 
                 Twilight, S260P634FN16.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P65FO14.T0 
               
               
                 BIEC122736 
                 chr10 
                 77229308 
                 A 
                 G 
                 0 
                 0 
                 Standardbred, Thorough 
                 S260P669FC24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P6113RJ4.T0 
               
               
                 BIEC134739 
                 chr11 
                 25480666 
                 T 
                 C 
                 0 
                 0 
                 QuarterHorse, Standard 
                 Twilight, S256P625RN3.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S260P6135RK10.T0 
               
               
                 BIEC150644 
                 chr11 
                 54946970 
                 T 
                 C 
                 0 
                 0 
                 AkalTeke, Arabian 
                 S259P682RB14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P6131RF14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S259P681RC6.T0 
               
               
                 BIEC157008 
                 chr12 
                 5419708 
                 T 
                 C 
                 0 
                 0 
                 Andalusian, Standard 
                 Twilight, S257P633FK3.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S260P625RJ22.T0 
               
               
                 BIEC161407 
                 chr12 
                 10322290 
                 A 
                 G 
                 0 
                 0 
                 Andalusian, Quarter 
                 Twilight, S257P639RP9.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P68RP19.T0 
               
               
                 BIEC169520 
                 chr13 
                 2271955 
                 A 
                 G 
                 0 
                 0 
                 Standardbred, Andalusian 
                 Twilight, S260P618FF19.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P668RB15.T0 
               
               
                 BIEC171790 
                 chr13 
                 6672587 
                 G 
                 T 
                 0 
                 0 
                 Thoroughbred, Akal 
                 Twilight, S261P637F116.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Teke, Icelandic 
                 S259P692FD1.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S258P63FG24.T0 
               
               
                 BIEC198778 
                 chr14 
                 37386709 
                 G 
                 C 
                 0 
                 0 
                 Arabian, Andalusian 
                 Twilight, S255P659FC13.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P614RK2.T0 
               
               
                 BIEC219170 
                 chr14 
                 91058897 
                 T 
                 G 
                 0 
                 10 
                 Arabian, Standardbred 
                 Twilight, S255P674FO17.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S260P622RE1.T0 
               
               
                 BIEC238830 
                 chr15 
                 25486024 
                 G 
                 A 
                 0 
                 0 
                 Icelandic, AkalTeke, 
                 Twilight, S258P6117FA8.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Thoroughbred 
                 S259P640FM2.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S261P625RL10.T0 
               
               
                 BIEC263072 
                 chr15 
                 78561852 
                 C 
                 T 
                 0 
                 0 
                 Thoroughbred, Quarter 
                 S261P6134RL4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P651FB4.T0 
               
               
                 BIEC281414 
                 chr16 
                 39841120 
                 G 
                 A 
                 0 
                 0 
                 Arabian, Thoroughbred 
                 Twilight, S255P657FI17.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S261P612RK6.T0 
               
               
                 BIEC193770 
                 chr16 
                 61569545 
                 A 
                 C 
                 0 
                 0 
                 Arabian, QuarterHorse 
                 S255P638RI15.T0, S256P6141FM5.T0 
               
               
                 BIEC317564 
                 chr17 
                 59719397 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, Arabian 
                 S261P641RI19.T0, S255P649FG19.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P664RN14.T0 
               
               
                 BIEC324581 
                 chr18 
                 140840 
                 T 
                 C 
                 0 
                 19 
                 QuarterHorse, Icelandic 
                 Twilight, S256P620RN20.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S258P618FJ22.T0 
               
               
                 BIEC328312 
                 chr18 
                 3797273 
                 G 
                 C 
                 0 
                 0 
                 QuarterHorse, Thorough 
                 Twilight, S256P663FF5.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P67F11.T0 
               
               
                 BIEC343880 
                 chr19 
                 2139046 
                 G 
                 A 
                 0 
                 23 
                 Thoroughbred, Quarter 
                 Twilight, S261P6104RE8.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P6142FG11.T0 
               
               
                 BIEC358651 
                 chr19 
                 39747577 
                 C 
                 G 
                 0 
                 0 
                 Arabian, Thoroughbred 
                 Twilight, S255P6104FA7.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S261P672RF7.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P6104FK20.T0 
               
               
                 BIEC425746 
                 chr20 
                 7131135 
                 G 
                 T 
                 0 
                 16 
                 Arabian, Thoroughbred 
                 Twilight, S255P628RJ1.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S261P619FL19.T0 
               
               
                 BIEC441654 
                 chr20 
                 40579748 
                 G 
                 A 
                 0 
                 0 
                 AkalTeke, Thorough 
                 Twilight, S259P6109FH22.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P626RB20.T0 
               
               
                 BIEC455646 
                 chr21 
                 6675166 
                 A 
                 G 
                 0 
                 0 
                 Thoroughbred, Akal 
                 Twilight, S261P643FK9.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Teke 
                 S259P612RO15.T0 
               
               
                 BIEC476263 
                 chr21 
                 48458497 
                 A 
                 C 
                 0 
                 0 
                 AkalTeke, Thorough 
                 Twilight, S259P634RE12.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P629RG8.T0 
               
               
                 BIEC480445 
                 chr22 
                 4678114 
                 A 
                 G 
                 0 
                 0 
                 Thoroughbred, Quarter 
                 Twilight, S261P695FM4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
                 S256P671RM7.T0 
               
               
                 BIEC500415 
                 chr22 
                 40767079 
                 A 
                 G 
                 0 
                 0 
                 AkalTeke, Andalusian 
                 Twilight, S259P672FK10.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P685RC14.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P699RA5.T0 
               
               
                 BIEC514026 
                 chr23 
                 18527196 
                 A 
                 G 
                 0 
                 0 
                 QuarterHorse, Arabian 
                 Twilight, S256P635RJ23.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P6123FA8.T0 
               
               
                 BIEC526317 
                 chr24 
                 16363399 
                 C 
                 A 
                 0 
                 0 
                 QuarterHorse, Thorough 
                 S256P63FO14.T0, S261P635RL3.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred, Standardbred 
                 S260P646RA8.T1 
               
               
                 BIEC542390 
                 chr24 
                 45955554 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, Standard 
                 Twilight, S261P631RC21.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S260P6112FC19.T0 
               
               
                 BIEC544278 
                 chr25 
                 1928873 
                 A 
                 C 
                 0 
                 0 
                 Thoroughbred, Andalusian 
                 Twilight, S261P619RD12.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P6104FE8.T0 
               
               
                 BIEC555903 
                 chr25 
                 23640999 
                 A 
                 G 
                 0 
                 8 
                 Arabian, QuarterHorse 
                 S255P636FN18.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P630RC4.T0 
               
               
                 BIEC562394 
                 chr26 
                 866401 
                 A 
                 G 
                 0 
                 0 
                 Icelandic, Thorough 
                 Twilight, S258P69RH5.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P660FD13.T0 
               
               
                 BIEC580675 
                 chr26 
                 38547619 
                 T 
                 G 
                 0 
                 0 
                 Arabian, Standardbred 
                 Twilight, S255P614R16.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S260P672FF19.T0 
               
               
                 BIEC590604 
                 chr27 
                 18662204 
                 C 
                 T 
                 0 
                 0 
                 AkalTeke, QuarterHorse 
                 Twilight, S259P6119RJ11.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P662RG24.T0 
               
               
                 BIEC600682 
                 chr27 
                 33575633 
                 G 
                 T 
                 0 
                 0 
                 Icelandic, Arabian 
                 Twilight, S258P613FK19.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P626FP3.T0 
               
               
                 BIEC622581 
                 chr28 
                 36023181 
                 T 
                 G 
                 0 
                 0 
                 AkalTeke, Arabian 
                 Twilight, S259P645RI2.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P6107FP11.T0 
               
               
                 BIEC633730 
                 chr29 
                 12652411 
                 G 
                 A 
                 0 
                 8 
                 Arabian, QuarterHorse 
                 Twilight, S255P648RL24.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S256P624RP9.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S255P688FO12.T0 
               
               
                 BIEC637205 
                 chr29 
                 20034612 
                 C 
                 T 
                 0 
                 0 
                 QuarterHorse, Thorough 
                 Twilight, S256P618RB4.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                 bred 
                 S261P625RJ24.T0 
               
               
                 BIEC687178 
                 chr30 
                 2391118 
                 G 
                 A 
                 0 
                 0 
                 Arabian, AkalTeke 
                 Twilight, S255P649F023.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S259P6119RJ13.T0 
               
               
                 BIEC706272 
                 chr31 
                 18788056 
                 G 
                 T 
                 0 
                 16 
                 Thoroughbred, Andalusian 
                 Twilight, S261P642FF10.T0, 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S257P647FD8.T0 
               
               
                 BIEC942271 
                 chrX 
                 14686957 
                 T 
                 C 
                 0 
                 0 
                 Thoroughbred, Quarter 
                 S261P61FA21.T0, S256P612RL8.T0 
               
               
                   
                   
                   
                   
                   
                   
                   
                 Horse 
               
               
                   
               
             
          
         
       
     
         [0078]    The nucleic acid sequences of the nucleotide markers of Table 3 are provided in Table 4 as follows, where the position of the polymorphic site (e.g., the position of the single nucleotide polymorphism (SNP), insertion and/or deletion) is bracketed and indicated in bold: 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 4 
               
               
                   
               
               
                 HORSE SNP PANEL (SET #2) NUCLEOTIDE MARKER SEQUENCES 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 BIEC20186 
                 AGCTTGCTAAAGTACATTTTTCTTTTTTTTGCAATTTGGAAACTGCATGTG 
                   
               
               
                 (SEQ ID 
                 ACTGATTGGCTTATCCAATCTGTAGGTAGTAAAAGATCCGTATTGTATTT 
               
               
                 NO: 65) 
                 GATGCCAAGCCCACAAATCCTCGAAAGAGGGAAGAATTCAGAAGGAAT 
               
               
                   
                 TACTGGACAGCTCACAAGGAATTTCAGCCTCACAAAACTTCTAAAAGCT 
               
               
                   
                 AG [C/T] TTCTAAGTAACACATTTTTGTCTCGCATTATATCTAAACCTTGGA 
               
               
                   
                 TAGGTTTTTCTTTTATGCAATGTAATAAANATTTTCCTAAAATACATAGC 
               
               
                   
                 CTGGCTATTCTATACTCGCTGGGAGGTTGCATTTTTACNACTTAACNAGT 
               
               
                   
                 AAAGATNAGGAAACGATCTTCATAAAATGTAAGCTAGAGACATGGCAC 
               
               
                   
                 ATGCATA 
               
               
                   
               
               
                 BIEC41954 
                 CAGCCCGGCATCTCTGCTTCGCTTGCTTTCTTCCCTTTTTTCCACCCGCTC 
               
               
                 (SEQ ID 
                 TTCTTCTTTCTTTTTTTTACACTTGGCTACGGAACATTAGATTTGACGAAG 
               
               
                 NO: 66) 
                 GGAAAGCGATTTAACATCTTTTAACTGAAGGAGGTTGATTTTCATTTGAG 
               
               
                   
                 ACCAAATGGCTCATTTTTACCTTTTTAGCTCCTGGAAGAGTAAAAAGT [G/   
               
               
                   
                   T] AGAGGTCAGCCCTGCGTCACCATGGCAACCCACACAGCTCGGGCCAG 
               
               
                   
                 GAGCAGATGAACGGAGGTGATAATCGGGGGGAAGTTTCTCCCTCTTCCT 
               
               
                   
                 TACATGGCATTTTCTTCCCATTCAGGGATGTGCCTGGCTGTATGAAAACA 
               
               
                   
                 ATTANGTCTTTTTGGAAATATAAGTTTTAAAATATTACTGCTAAATTGTC 
               
               
                   
                 TGAC 
               
               
                   
               
               
                 BIEC51268 
                 CCATTCACAGGAGGAGATTAGCATCTGGTCCCGCTTGGACTGTCCCCAG 
               
               
                 (SEQ ID 
                 CACTTGATGCATTCATGGCTGGTCTTGANCCTGCCCTGCTCCTCCCCTCC 
               
               
                 NO: 67) 
                 CATCGCCACACCTTCGTGTGTGCCGTGGCCCGTGCCTGTTCTTCCCTCTTT 
               
               
                   
                 TGTCCTGTCTGTCCCTCTGAATCCTTCTTCCCAAGGTGCTGCTCGTCCCT 
               
               
                   
                   [C/G] ACCTGTTCCCAAAGTTCCTCCCACTCCTGCAGCTCCCCTGGGCGCTT 
               
               
                   
                 CCTTCACCACTCTTAAGGGCTCTGAGGCATTGGGAAGTACCGTGAAGGA 
               
               
                   
                 CCCTAGGAAATTCAACTCCCTTGTTTTTACAGCTCTAGAAACGCAGGAGC 
               
               
                   
                 AGCGAGAAGTGACCTGAAGAAGAGCAGCAACGGTACCTCCTCGACTTA 
               
               
                   
                 GCTGTT 
               
               
                   
               
               
                 BIEC367927 
                 gagccaaactatatctttctcgccccaaacccaggctctCTTTCCCCAAAGAAAGTATTTGGTCT 
               
               
                 (SEQ ID 
                 TCTCCAAACCTTGAATGTCAGCCTTTCCCTTGGAAGTGTTGCTCAGACAC 
               
               
                 NO: 68) 
                 AAAATTTTTTTCACCTCTTGCCCATGAAAGTTTATATATTACTCCTGATAT 
               
               
                   
                 CAATTTTACAGTTTTTGCCCAAATCACCAGTAGC [T/C] CGATGATAGCAA 
               
               
                   
                 TTTTCAGACCCTCTATGAATTACAGGTGCGTGAAAATGGTCCCTTAGTTA 
               
               
                   
                 TTGGAGAAGAAATGAATTCAGGGTTAATCCATCTGACCTCTTTCTTCATA 
               
               
                   
                 GAGAGCAGAGTTTATAATAGACTTTTATGTTGAACTGTTGGTACATGTTT 
               
               
                   
                 TAAGAGGAGGAATGTATCCATACCTGGGATTTTAAATA 
               
               
                   
               
               
                 BIEC392749 
                 TGAGGGTGGAGGCAAAGTCATTTCAAAGTATTCNGGTATCTACTGGGTG 
               
               
                 (SEQ ID 
                 CCCTGGATCCAATATAGAACAGTACTGGTGGAGAGAGGAAGCATAAGA 
               
               
                 NO: 69) 
                 CAGGCAATTTTAGAAAAGTTCTCATGAGGGAAGTGACTGATGCATATGT 
               
               
                   
                 GGTACATAAGGTTGCNAGCCCGAAGGTCTCACTTAGGGACCCAGTCCCC 
               
               
                   
                 ATCAG [G/A] GACTTGGGTAAAGTCTGATACAGGATGCACCAGGGGGTCC 
               
               
                   
                 CCATGACCCGGAGTGGACTGAGCTCAAGGAAGACAAATCCAGCAGTCC 
               
               
                   
                 ACATCTTGCATTGCATTAGCAATGGTTTGTGCAATGCATACCAGGGAGTT 
               
               
                   
                 GGGAGTTGTCCAGTCAGGCAATAACTGGAAANTGTAAGGAATACAGAT 
               
               
                   
                 GAATTAGCAATGTG 
               
               
                   
               
               
                 BIEC654812 
                 CCACTCCATCCTCTGTGGTCAGACTTTTGTGTCTACACGTAGTTTTATGG 
               
               
                 (SEQ ID 
                 AGACAACATTTAGTTCTTCATTAATTCCGTCTGCAGGAGACTTCTACCTA 
               
               
                 NO: 70) 
                 CCCTGCCCACACACATCTAGGACATGCCCGATTATATGTTTCATTTTAAC 
               
               
                   
                 TCTTCAAACCCAGAAAACTGTCCTTCATTCATTGATTTATTTGTTGGTAG 
               
               
                   
                   [A/G] TTCCACAAGCATCCATGGAGTCTGTTCCATGCCTGGAATTGTACTAG 
               
               
                   
                 GTACTGGATACTAATAAGATGTCTGGGTGCTATCCCTGCCCCCAAGGGA 
               
               
                   
                 CCTTGAGGACAGTGGGAAATCCAGGTACATGGGTGATGGCAGCTCAACA 
               
               
                   
                 GTCACATCCTATTGTGATAATAAAGATTTATTGAGACTTTATGCCATGTT 
               
               
                   
                 CTAAA 
               
               
                   
               
               
                 BIEC683021 
                 CTGTGCTCTGGTGCAGATCCAAGGTCTTCGCAGCAAAGAAGGGAAGACC 
               
               
                 (SEQ ID 
                 GGAAGATGGACCAGGCTGTTGTGGATTTAAAAAGGGATTTTTGGAATAT 
               
               
                 NO: 71) 
                 GTTTCCCACATACATTGTTTATTCCTGAATTATCCTTTCCCAGGAAGCAG 
               
               
                   
                 TATATCCATACCTAGATCTTCATGCAGAAGAAAGAAACTGAGGAAGTCT 
               
               
                   
                 GTA [C/T] GTTTCCTTCTTTTAGAAGGGAGAGCCAGGGACGCTAATTGTTA 
               
               
                   
                 CTTAGTGGAAACTAAACTATATTTTCCCTTCCCCTGACAAATCCTCCCAT 
               
               
                   
                 AGTTTTCTTGCTTTGTCATTCAAAAAATACTGGTGTTTCTAAGAGCCTGA 
               
               
                   
                 GTTCTTGAGACAGTCTTTCCAGATGAGTTAAGGAAGAATCTAGAGAGGA 
               
               
                   
                 AGTCCCTG 
               
               
                   
               
               
                 BIEC674509 
                 AGCACACCAACAGCAAACGTTTCTGCAGACCAACTGGAGTTTAATGGTC 
               
               
                 (SEQ ID 
                 TTTCTCAGTGAAAAAATATTTAAATATCTATTGTTTATGCCTATTTTCATC 
               
               
                 NO: 72) 
                 AAATAAATTAGTGAAATTGGCGAGTTTTCAATCATTACTCATGTTAATAA 
               
               
                   
                 CCTTTCACTTGAACTCCCACTAAGGCTTCTGGTCATTAAAAATTGAACAC 
               
               
                   
                   [C/T] GGGGCTGGTTTGTGTTCCCTCCCCTGGCTTCCTCATTCCGCTTCTCT 
               
               
                   
                 TTTTCCTTTACATGGTGTCCAGTTCTCAAGCACACGTTCCCCCACCCCCA 
               
               
                   
                 CGCCTGACCTGTCACAACCAAACCTCAGGGAATGAGTCATTCATTCTCAT 
               
               
                   
                 TAGACGGTTGGTGACCACTTAAGCATTGCTGGGTCCTTTCCTGAGGTATG 
               
               
                   
                 TGG 
               
               
                   
               
               
                 BIEC724885 
                 ATCAAGATTTCCCCAACTTGAGAGTGAAAAGAAGGAGAGGTGAGAAGA 
               
               
                 (SEQ ID 
                 AGATGAGTTTGATTTCGAATGTGCTGAATTTGAAAAATCTCAAGATAGA 
               
               
                 NO: 73) 
                 GTGTCTAGAAGGGAGATATGGGAATGAGCAACACAGAGATAATAATTA 
               
               
                   
                 GAATGGATGATACAAGGGACATATTATAAAATGAAAAAAGATTTCACCA 
               
               
                   
                 TAAAGC [T/G] AAGATGTTGGATCATCTTGCTTTAGGCATACTAAGCTATT 
               
               
                   
                 CCTCCCAGAGACTGGTCACATATTGGCAGGAGAATCTGAAGACCTCTAA 
               
               
                   
                 TTCAACAGCAATAAAGAGAACTAAACAATGGGATCATGCTCCAGAGTTT 
               
               
                   
                 GGCGTCTAGCCTTAGCAAACTCGTTTGTAATGAAGGCGCCAGCTTTGTA 
               
               
                   
                 GTTAAACATACCT 
               
               
                   
               
               
                 BIEC744445 
                 ACCCACAGTCCTTTTGGCGAATTCTTATCTCGTGGGGCAGTGCAGACAAT 
               
               
                 (SEQ ID 
                 GGGGGTTTGTGCTGACGGCTGCTGGTACTCTGCTAATAGTCCTGGAAGCT 
               
               
                 NO: 74) 
                 TCCACGTTGCTTATTTGGGCATCTTTTCACACCATCACCTGCAACCAGCA 
               
               
                   
                 GTCCCTGGTGATCCCGACTTTGTCAGCCTGGCACCCCCATCCAGATACGT 
               
               
                   
                   [C/G] CGGAGGAAAGAGCTTGGTCTGAATGGGGGCGGAGGGGAGCTGAG 
               
               
                   
                 GAGCACCGGGAGGAAGGAGTCAAATACTGTTATCTGGGTGTTTTCGCAT 
               
               
                   
                 TTTGTTTCTCCTGCACACCCTTCCCTCCCCTTCCCACCCCCGCTCCCAGTA 
               
               
                   
                 TCATTTCTCTTTGAAATGTCATGAACTTGGCGCTTTCAGACCAAATCGCC 
               
               
                   
                 GGGCTC 
               
               
                   
               
               
                 BIEC745623 
                 GGAGAGGAGNGCAGGGAAGACAAATCGCCTGTTTGCTTGAAAGGGAAA 
               
               
                 (SEQ ID 
                 GGCTACAAATCAACGCTGGGGGAGCGAGAGGAAGAGGGGGGCAAAGGT 
               
               
                 NO: 75) 
                 CAGGGGTAGGAACGAGGGGGAGACAGGGCAGCTACAGCGCAGGAGTAG 
               
               
                   
                 GACAGGAAGCATTTAAACGAAATCCCAGTTTTACACCTAAAAATATAAA 
               
               
                   
                 GCTGTCA [A/G] TTTTCATTAAGGATGAAGCAAATGAAATCTAGAGGGTGC 
               
               
                   
                 CAAATAATTAATGACTTTTAATAACCCTAATAAATTTGGATTTCATCAAA 
               
               
                   
                 ACCATGTTGCCTATCATGGACGATCACCAGCAAGAATGGGGAGCTGTCA 
               
               
                   
                 AGGGGGGCTAACACCTGTGTGAAAATGGACGCCNCTGACATCCTTCCCA 
               
               
                   
                 GGTACATCAATTA 
               
               
                   
               
               
                 BIEC751023 
                 TCCACGCAACGGTTCTTCTCTGAACAGCAACAGAGCAAACAGATAGGAG 
               
               
                 (SEQ ID 
                 GCAAAGCTCAGAAAGTGGACAGTGATTCCAGTAAACCCGAAGCGCTGA 
               
               
                 NO: 76) 
                 CTGACCCTCCTGGTGTCTGTCAGGAAAAAGGAGAAGAAAAACCACCTCC 
               
               
                   
                 TGCACCTGCCCTAGCCGCCAAACCTGTCAGAACTGGACCCATCAAGCCT 
               
               
                   
                 CAGGC [G/C] ATCAAAACTGAAGAAACAAAATCTTAAAGGCTGTGGTTTA 
               
               
                   
                 TTGCCAGGGATTGGGGGAGGGGAGAGGGGAACGAGGGAGAATGAAGTC 
               
               
                   
                 AGATAATGCCAGCAGCCAAAGGGGTAAAACGGTCTGTGACATTATCCTG 
               
               
                   
                 TCCAGAGCTTGGAGGTGCACAAGGGACATAGGAGCAATTTACACTGACA 
               
               
                   
                 CACAGCTGCTACAC 
               
               
                   
               
               
                 BIEC758316 
                 taataaaccactttccTCATTTCAGTACTGATAGGCTTATGGGAATATGCCATCTTT 
               
               
                 (SEQ ID 
                 GGAATCTAATCTTTGCATTATCTTATCTATCCTTNGTATTATCTTTACAAC 
               
               
                 NO: 77) 
                 CAATGTTATAAGGCCATAGAAACAGAGTATACCCTCTCATGGGCAATGT 
               
               
                   
                 GTGGTAAATTTTCCCCCTTTGTTTATTTCTCTTCAGGCCTTCA [A/T] TGTA 
               
               
                   
                 GCACCCTCCCAAACCATCCCTTCCCGCTCAGCCTACACACCCTTCACTCT 
               
               
                   
                 TACAGTTCAAATATAGTACTCCTAATTTTTACTGAAAAATAAGGCCAGG 
               
               
                   
                 ATTTTTTCACTCTCACTTGCTTCTCTTGCCACTCTGGCCACAACAATTTAC 
               
               
                   
                 ATGCCTctcacaaaagcctcatctcattatatcactggctcaaagt 
               
               
                   
               
               
                 BIEC784849 
                 TCNTAGGACAAGACTAGTGGGGAGGAGACCTGAACTCCAGTGAATTGTC 
               
               
                 (SEQ ID 
                 CCATCAAGTTCATTATCTCTGGAATTGTTTTTACTCATGGGAGTTCTTAC 
               
               
                 NO: 78) 
                 AGTAACTCCTATAAGGAGGTTAATGGGAGGGAGAGGTGGCCTATGAAG 
               
               
                   
                 NCAGGGAGAGGGGAATCCTGACAGCGGAAATAAACCCTCTCCACAAAA 
               
               
                   
                 GTCAG [G/A] GCTGTCTATTGACAGAAAGGCTGTGTGTGTGTGTGTTGCAC 
               
               
                   
                 ACGCATGAGTATCGATGTGTATATGTGTGCGTTTCTCATCTACTTCTCCT 
               
               
                   
                 AAACTTGCTCTCAGAAAGAACCACTTTTTCTTCTTTTTCTTTTTAACGTAT 
               
               
                   
                 TTGGTTTCCACTAAATCAGGATTAGTGGCCATATTCAGCCTCGAAAGAC 
               
               
                   
                 AGTTGGAAG 
               
               
                   
               
               
                 BIEC787485 
                 TTGTAAAAAGTTTCAACTTTAATTTTAATTGAAGTGCAAACTACAACCAT 
               
               
                 (SEQ ID 
                 GAGATATGCTTTTGTTTATCAGATTGGCAAACCTTAAACACATCCTTTAT 
               
               
                 NO: 79) 
                 CAGTGGTTCATCTACTGCTGGTAGGAAGGAAAAAATGGTACAGTATTTC 
               
               
                   
                 TGTTACTTCTGGTGTGAATATTATTGCCAAAATAAGATTTAGAAGATAAA 
               
               
                   
                 A [C/A] GGGTTTGTGGACTGTGTTTTGTGATGCACCATGTGATTGCAGACT 
               
               
                   
                 GCTCTTGTTTTTTCCAGTGATAAAAAGTTGATTGCAGAAGGCCCTGGGGA 
               
               
                   
                 GACAGTGCTGGTTGCGGAAGAAGAAGCTGCTCGCGTGGCGCTTCGGAAA 
               
               
                   
                 CTCTATGGGTTCGCTGAGAATAGACGGCCCTGGGACTATTCCAAGCCCA 
               
               
                   
                 AAGAGGG 
               
               
                   
               
               
                 BIEC818096 
                 CAGTAATCACATTCTGGCTTAGCCCTTCCGTAGAAGCCACAGGCAAAGG 
               
               
                 (SEQ ID 
                 CAATGGTCTCTGTTTTCAGTTTCTGCCATGAAGAATCACAAGTCTCTGGA 
               
               
                 NO: 80) 
                 GAGGACACATGTCTCTTAGGTTTACAGAACATTCAAGAAACAAGGCTGT 
               
               
                   
                 GAGTTTGGGTCCTAAGTCACGGTGGTCACTTGAAGACGTGGTTCTGGAA 
               
               
                   
                 GTC [C/G] CCATTTCGCATTAGCCACAGTCCAGCTTTGCCACAATCCAGAT 
               
               
                   
                 TCAAAAGAGACGTATTCCGGCAATTCTCTGAGAAACATACTGCATATGT 
               
               
                   
                 TGCTGCATTAGAAACTAAGCCAGGCCCCACAGGAGGCCCTAAAGAAATG 
               
               
                   
                 GGGCTCAGGCGCGTGCAGACACAAGGGGGTGGTGAGAGCTGTTATTCGA 
               
               
                   
                 CACGTGCACT 
               
               
                   
               
               
                 BIEC818829 
                 AGCCTGCCATGCAGGAGCAGATGCCCACTCCCGGGGACCCNCAGACACC 
               
               
                 (SEQ ID 
                 CCCACACCCCCAGCCAATCCTGCAGCTCCTCCTGTGCAGCCCCCCGGCTC 
               
               
                 NO: 81) 
                 CCCATGCCTGCCCCCACCTGCTCATAGGCCATGAACTTGATAGCCGACTC 
               
               
                   
                 AGGGGCAATCTTGAGCACGTTGATCCCGTTGCCACGCCACAGGGAACGC 
               
               
                   
                 AC [G/A] CCCCCTTCTCGGATCATGCTCCGTAGGCCCCCCAAGATATTCAG 
               
               
                   
                 CCGGTTGGTCTTGGAGGCGTGGACCTGCGCGGGGAGACGAGGTGGCCTT 
               
               
                   
                 GGGGTCCCCTCCGGGGGGCCCAGGCCCAGGAGGAGGTGGGGGTTCCTCC 
               
               
                   
                 AGGCCCCCTCACCTGCATGAAGACCTTGAGGCGGTCCAGAGGGGCGGTG 
               
               
                   
                 CCTGTCCGA 
               
               
                   
               
               
                 BIEC837622 
                 GTTCTGGACATCACTGTACTTCAGCAATAAGTGGTGTGTGTGTGCGGAGT 
               
               
                 (SEQ ID 
                 GGGGTGGCGGATGGATGGAGGCTTGGAGAGGGGATGATAGGGCTTCTA 
               
               
                 NO: 82) 
                 GACCGCCAGGAAAAATCCCCCCATGACATGTGGGGAGAGGCCTCCATTG 
               
               
                   
                 GCCAGCTCTTTGCCTCCACTTCCAGGAAGGAGATGGATGGTGTTTACCTC 
               
               
                   
                 CCG [C/A] TTTCCACGCCCTGGCCGGGACTGTACCAGAACAACTCTACAAG 
               
               
                   
                 GAACAGGATTCACCCATGCTGGCCACTATTTCCATGGCTTTAACTTGTCA 
               
               
                   
                 CCAGTGTACCAGGGAAAGCTGACACTTATTTAATCCTCACAGTGGTCTTG 
               
               
                   
                 ACTGTGTACTCACGAAAACATTGTATGCTTTTGGAAGACGTTTGTTTCCA 
               
               
                   
                 AGCAGCT 
               
               
                   
               
               
                 BIEC855571 
                 CTTGGTACTCTCATCTTAGAGCCTATCTTACATGACTGTGGATCTTAACT 
               
               
                 (SEQ ID 
                 TACTGTTAAGTAGTTACTGAATGCTGACTATGCNATGGACCCTGTAATGG 
               
               
                 NO: 83) 
                 ACACAAAGAGGAATAAGGTGCTGTCCTGCCTGAGGGACCGAGAGGCTA 
               
               
                   
                 TTCAGAGACCCGTGTGTTCAAATGTATCTATTTACATCCCACAGTCAGCT 
               
               
                   
                 GT [C/T] TCCAAAGTCAGGGGGGCTAGGGCAGCTCACAGAAGGAGGATGC 
               
               
                   
                 TATATGTGCTAGATTGAGTTAGGCCAGTCCAGAGAGGACATGGCACTTG 
               
               
                   
                 GCAGTTGAATTGCTGTCTAGGGAGAAGCCTAAGAATTCATTCACTCCAC 
               
               
                   
                 AAGTATTTATTTTTAATTTATTGTGTCTCAGAAACAAAAAGGGAAAATTA 
               
               
                   
                 ATTTAATTA 
               
               
                   
               
               
                 BIEC859610 
                 tgaggcaggacttccctgagctccgagatgcttgtgtgcactgCACTGTCCAGGGCGCTTGGCTGT 
               
               
                 (SEQ ID 
                 TTCCCAGGCTCGCGAGAGCATGGCNTCTGATTTTTACATGGCCTCCTGTT 
               
               
                 NO: 84) 
                 TAGCCCCCACACCCCCTCACACTCCCTCACACTGTTCTGGCGCTGCTGGG 
               
               
                   
                 GCAGACGTATGGAGGAATAATCATCGTGAGGGGC [G/A] TTGAACTCAGG 
               
               
                   
                 GAAGGTACCAAAGTGCATTGTGGGGTTTGGCCCCAGTGAGCTTAAAACA 
               
               
                   
                 GTCTTTTTCAGTGAGCTCTGAACCCCTTCCCGTCCCAGTGCTGTTCTTGTT 
               
               
                   
                 CAGAAGCCTGGGATGAACCCCAGCTTCTTTCCAGAGACGATTTCAGGCA 
               
               
                   
                 CACAGGGATCTTTTATCCTTGTTTCTCCTGGTACCTTGGA 
               
               
                   
               
               
                 BIEC870924 
                 TTCTGTTTGTTTAGAACNGCCTGATGAGAGAATTGGATCCTGAGCTCTCA 
               
               
                 (SEQ ID 
                 TAGGGACATCGCCATAAAATCATCTGCCCGTATCGTTGGAGAGTGGGAA 
               
               
                 NO: 85) 
                 AACCTTCCTCNAGAGAGTAAATAGTCTAAACAACATTGTTTAGATTTAG 
               
               
                   
                 NTAGTCTCGTTTATCCACAGCTCAGAGACCTAAAATAGTTCTGCAAAAG 
               
               
                   
                 GAC [A/G] CATGAGTAGGAAAACCCCTAGGCTCCTAGGATGCCGTCTGTG 
               
               
                   
                 GCCCAGGTGGCAGGTGTCCTCCCGGAACACCCTGTGACCGGGAGGANTC 
               
               
                   
                 ATGGGAAAANGAGGCTCTGCNGAAGCCACCACCNCCTCCCAGAACCTGC 
               
               
                   
                 TGTCCAGGAGCCCACTGTTTATTTCTATTTTTTCACTTCATTTGTTTTTAA 
               
               
                   
                 TACTAGGAT 
               
               
                   
               
               
                 BIEC888973 
                 gatatgtttgaggtggtggtttcagccattttggtgagttactcagcttgcccgagcaactcccatgtatacatTGACA 
               
               
                 (SEQ ID 
                 AATAGAAATGGCAAGTAATAGGATATATGGGAGTACATGAGGAAGCCA 
               
               
                 NO: 86) 
                 TTTGGTGTAAACCTAATTCGGCCTGACTTTGCTATTTTTTCCAAAAGGGC 
               
               
                   
                 CTGACCGAGGCTGTTGAGCATGC [A/G] TTGTATATCTGCTTTAGATATTC 
               
               
                   
                 CCTATGGCAAGAACAAAGGCCCTTGAGATAAAGGTGCAACTTCCCTCNC 
               
               
                   
                 CCTCCCAANGTAGACATTTCCTTAAGGATTAAGCATCTTTCCTTAGGCTA 
               
               
                   
                 GGAACTGATTGCTTTGCTTACCTGTGACCACCCAGCTGGAGACAATAGA 
               
               
                   
                 CTTGCCTCCTGCTACGCCCACAGAGATAG 
               
               
                   
               
               
                 BIEC921711 
                 CCAAACTGAACTAAGAAAACATGTGGTGGCGAGGGGGCATCTGGTGCTA 
               
               
                 (SEQ ID 
                 TAAGACANGGGTGGAGGAAACCACAGCCCCAATGCTGTAGAGTGACTC 
               
               
                 NO: 87) 
                 GATAAGCTTGTCCCTGTGGGAGCGAAAGGAGAGACACAGGGAGAAGGG 
               
               
                   
                 CATCTGAAAGGGGTCTTCTCCTGAAAGAGCTTCATTCCCCTCCCCTCTGG 
               
               
                   
                 CGGGC [T/C] GGATTCTTTGCACAGATAATTCTAAACCTGTCTTTCCCAGC 
               
               
                   
                 ACTTTCGGCTGGAGAGGTAGTCTGCAAGGTTTTCTGCCTTATCCTATTTT 
               
               
                   
                 ATTACCAACCTACCTCCACACATTCCAGTTTTGAAGAGAAAACTAATAA 
               
               
                   
                 GGAAATGGCTGAAATAGTGTCTTGATGGCAGAAGGAAGAAATAGCTCCT 
               
               
                   
                 TAAAATTTGTT 
               
               
                   
               
               
                 BIEC930198 
                 AGCATAGCTTAATAATACACTTCATTTTCACATTTATTTACTTCAAAGTG 
               
               
                 (SEQ ID 
                 TATTTTCATGTTTTATGTACTTATCTATATTTTTGCTTTTAGCACAAAGAA 
               
               
                 NO: 88) 
                 CAGAAAAGTAAGATTACCACAGCATTCCAATATGTTAACAAAATTTGGA 
               
               
                   
                 ATGAACTATGGATCAAACCTTGGGCACTGAAACTCTTCAGGGCCCTTTA 
               
               
                   
                 A [A/G] GAACAGTTTGAAAAGGTTCTGTGAAGTTTNAATAGTTAGGCTAG 
               
               
                   
                 AAGTCCCTCAAAGAAGAGGAATAGAACGCACAACCTCCCAAATAGCAG 
               
               
                   
                 AGGCAAAAGAAGAGTGAGAAACCCACAGCAATCCTAAGGAGAacaaactaca 
               
               
                   
                 tattttactgattttttttttcccatcttcctcaattagaatgtaatcgctaccaa 
               
               
                   
               
               
                 BIEC111585 
                 TTGATCTGAAGCTGTAAATTCCCCAGTAGCCAACCCTGTTGACTCACCTG 
               
               
                 (SEQ ID 
                 ATACTTCATGATCAGGCAGACTAACTTGCTTCCAGTCAAATAAACAGAA 
               
               
                 NO: 89) 
                 AGGAAATTATTTGAAAATCAGGAAAAAAATAATCTGGGATAGTCNGTGA 
               
               
                   
                 TCTGGAGTTGTCAGTAAGAAAAACTAGCATTTTAGAAATGTTTAATTTAT 
               
               
                   
                 CC [G/A] TTGACATTTTGTTCTGATTTTTCTGTGTGTGTTTGTTTGTTTTTCC 
               
               
                   
                 TATTTAGGAGAAAAGGGAAGATGTAGCCATTTTAAGAAAAAATAGACCC 
               
               
                   
                 ATTGAACAAATCTGAAAATATCAGTATGTATGCTGATGGCAGAAGAGTA 
               
               
                   
                 TAAGAGAGACTCTGTATTTGTAAACATAATTGATGGTTAAGTAAAGTAC 
               
               
                   
                 TCAATAA 
               
               
                   
               
               
                 BIEC122736 
                 TCTAGAGAGAGAAGTGTGCTTATCCTGAAAGTAAAAAAACCCAGGAGA 
               
               
                 (SEQ ID 
                 GAATAGGAAAGGACAAATATTTTTGGTAGAACCTGGTTGATCTGAACTC 
               
               
                 NO: 90) 
                 AGAATCTCCTGGAGATTCACGGGGTTCCCCGGCTTGGAAGGACAAAGGG 
               
               
                   
                 AGAAGCCAGCACCTGGAGCAGAGTGACCGTCCTTTGTGATCTGTGTGCA 
               
               
                   
                 CCACG [C/T] GTCAGAAATGTACCCAGCCAGCCACCGGATGTCACTGTTCT 
               
               
                   
                 CTCGTGCTCCTCGTCTGGGCATCCCACAACGTCTCTGCGTGAATNTTCGG 
               
               
                   
                 TGTCCTGCTTTGCAGCCTGACAGTGGCGCAGGCTCACCCGCACGGGCCT 
               
               
                   
                 CGCAATGTGGGATCCCTGACTATCCCCACATAACCATGATTTTCGTTCCA 
               
               
                   
                 GAGCAGCACA 
               
               
                   
               
               
                 BIEC134739 
                 CTATAACGGACTCCATGANCTGAGAGCTCATGTTTCAAACGGCAGGGNG 
               
               
                 (SEQ ID 
                 GGGGAGTGGGAAGTCACTAGATTGCTGGTTCTTAGACAGTCTTAGGGCC 
               
               
                 NO: 91) 
                 AGTTCTCATTCTCTCTCCTGCGCTGTAGGCTTCTGAAGACCTCCTCAAGG 
               
               
                   
                 AACACTACAACGACCTGAAGGACCGTCCGTTCTTTGCCGGGCTGGTGAA 
               
               
                   
                 ATA [C/T] ATGCACTCGGGGCCAGTGGTTGCCATGGTGAGTGTGCACGTGT 
               
               
                   
                 GGGAAGTCACTGTGAATGGCTCAGTTGGGGGTGAAGGGTGGGTGTTGTG 
               
               
                   
                 ATTCCTGCTTCTCATGCCGGGTCCTCATGGATGCGGAGCAAGCTGGGGCT 
               
               
                   
                 GGGAGGGTTAGACANTTAGGATGTGTCAGCTCGCTGACGCACCAAAGAC 
               
               
                   
                 AGGATGAAC 
               
               
                   
               
               
                 BIEC150644 
                 TAATAGGTTCAACAAAGTTGTGGCTGACTCCTTTAGTTGCTTATCCAAAA 
               
               
                 (SEQ ID 
                 TCCATTCCCTACTTCTTCCTCAATTTAACATTAATTTGATTTGGGACAGCA 
               
               
                 NO: 92) 
                 ATAGGTTTAGGTCCACAGTGATGGAATGGGACCAGCCTAAACTAATCAC 
               
               
                   
                 AGCCTCCCTTGCAGCTACAGGTGGCCAAATCAATGCATATAGCCATAGA 
               
               
                   
                 A [C/T] CTCTTCTTTCAGAGATCTCAGGCATACTTTTAGGAGAGTATGCTT 
               
               
                   
                 TGAGACTATTGGACCTAGGCTAACTGACTTACAAATTAGATGAACGCAC 
               
               
                   
                 TTCCTGACCAACCAAGCCAGAATGTATTCATCTTTTAGGCATTTCTCCTA 
               
               
                   
                 TAAAACATTTACTCTGGTTTCTCTCATTAAATGAAAACCTATGCACTCAT 
               
               
                   
                 TCATG 
               
               
                   
               
               
                 BIEC157008 
                 TCTTACTTTTATCCACAGAGCTTAAGAAGTTAGTCGGGCCAAAGACTGCC 
               
               
                 (SEQ ID 
                 CTCTACTTCTCCCTAGCTCACTATTACCCCTCAAGAAGACCTTGGGTGAA 
               
               
                 NO: 93) 
                 TGGCCCATCCAAGTTCTGCTCTGAGCAGAGAGACAAAATCCTTCCTTCAT 
               
               
                   
                 TGATATGATCTTCCTTTAAGGAACTTTCCCCAGGATTGGCTCCCCTCACC 
               
               
                   
                   [G/A] CTGACACTGAAGAAGTAAGGGACTTAGGGCCCAAGAAAAGCCTNC 
               
               
                   
                 CTCTAGCTGAGACAAAGAAAATCTCTTCATAATCCAACATCAGGAGACC 
               
               
                   
                 ACATGGAGGATGAAGCAAAAGCTTCAAAGACTGTCATAAAGATTGTGCA 
               
               
                   
                 TTCACTCAGCTTTGTCCTTTGTTGGAGACCTCTCCAGGGCATGACTAAAG 
               
               
                   
                 CAGGACA 
               
               
                   
               
               
                 BIEC161407 
                 TGTAATCAAAACTACAGGGCCTGACGAAGGAATTCAAATTCACAACCAA 
               
               
                 (SEQ ID 
                 CTTTTGTGACTTCTGGAAGGATAACCATAGTGACTTCCTGTAATAAAATC 
               
               
                 NO: 94) 
                 AGCCCCTGACCACCAAGAGAAGCCTGTGGAACCCCGAGACTTCAAGCCT 
               
               
                   
                 GTAAGGACAGCTTCAGCCAGTCCCTTATCATTTAGTAGAAAACCCCTTA 
               
               
                   
                 GGA [G/A] CTTCAGACATGTACTAGGACTTTTGGCCAATCTATACACTGAC 
               
               
                   
                 TAGAAAACTTATGCATAAGTTCAGCACAACTATTGTTCTTGATATTTAGA 
               
               
                   
                 GGGTCCTCAGGAAGGCCATGTGCTGGAAACAGAGTATGGCTAGGAAAG 
               
               
                   
                 TTCTTTGGAGGGAAGGTGGACTTTCCACCCCCTGCTCAGGGGCAGTGTTC 
               
               
                   
                 TCTGTTCCT 
               
               
                   
               
               
                 BIEC169520 
                 GACACTGGAATAACAGCAAGACCATGCGAAACATGGACGACACCGTCC 
               
               
                 (SEQ ID 
                 CTGCCCTCCTGGAGGTAACGACATGAGGAAGGGGTTATACGTACAGGCC 
               
               
                 NO: 95) 
                 ATGTGGTTACGAGTGTCCAGAGGTTGGCAAGGGGCTGCCCAGGATCCAG 
               
               
                   
                 AACCCCCAGAAGCTGGGAAGCAAAGCAGATTCTCACACACCCTCACTCT 
               
               
                   
                 CTCCC [T/G] GGCTCAGTGCAATAAGCTGTGAGTGCAGTCAGCTATTTCCT 
               
               
                   
                 TAGAAGACTGATAAATTGATAATTACACAGAGATGTAACACATATAATT 
               
               
                   
                 AAGCAATATTCTACAGGAACAGGAGTTTTGCTGCTTTTTTCCTTAATCAA 
               
               
                   
                 ATTATTCCTTTCTAAAGGTCTCTGTTCTGAGGAAGAAGAGACAGGACAC 
               
               
                   
                 CGATTCTAAGC 
               
               
                   
               
               
                 BIEC171790 
                 CAGGTAAGCCCCTTCTACTTTGTTCGGCCTCTTCTTTCCCTCCCTTATGCT 
               
               
                 (SEQ ID 
                 GTCAGTTCTCTCCCAGCTCTGCCCTCTCTTCCTGTAGACCTGGGTGAATG 
               
               
                 NO: 96) 
                 AAGGCTACTTCCCGGATGGTGTTTATTGCCGGAAGCTGGACCCGCCCGG 
               
               
                   
                 TGGACAGTTCTACAACTCCAAACGTATTGACTTTGATCTCTACACCTGAG 
               
               
                   
                   [C/G] CTGCTGAGGGCCCGGTTTGGTGGCCCCTTCTTTCCTGGACNCTGTG 
               
               
                   
                 GAGGAGGCCCCACGTGCCTCAGGCAGTGAGGATATTGGGGGCCACTTTT 
               
               
                   
                 CAGTCAATTTTCCTTTCCCAATAAAAGCCTTTAGTTGTGTATTATGGCCTT 
               
               
                   
                 GGCTGTGCTGAGGGCCAAAAGCCTTCTTCACAGCTCCNGTGGACTCACC 
               
               
                   
                 TCCAT 
               
               
                   
               
               
                 BIEC198778 
                 ttttagggaggtggtgggagctgggtggacaggagaagggaGACTTTTCTCAGTATAACTTACT 
               
               
                 (SEQ ID 
                 GCTATTTTAAAATTTTTGAACCATCCGAATGTATTACCTATTCAAAAAAA 
               
               
                 NO: 97) 
                 TTAGATGACAAAAGTCCCCACAAGGCTAGAATAAGAGCAAACTAAACA 
               
               
                   
                 GATAAATTCCATGCTGGTGCAGTAAAATGGAGACAAGT [G/T] CCAATCTC 
               
               
                   
                 AGAAGGTGACCCTGGCAACAAAGTTGTGGATGTAGGCTAGCTAGCCCTG 
               
               
                   
                 GTCACTGTAAACCAATGAAAGAAATGTGTGGATGGAGGATGGGCATAC 
               
               
                   
                 ACACATGCACACACAATGCCTTACCAGACACAAGCTGGAAAGGGTTCCC 
               
               
                   
                 ACAATTGGAGATGTCTGCATGATCAGGCAAAGGCTAAGGGAGAGAG 
               
               
                   
               
               
                 BIEC219170 
                 GAGGAAGTAGAATTCAAGGAGGAAAAAAAATCCTAAGACGTTAGTTTG 
               
               
                 (SEQ ID 
                 TGAAACGTGAACAGAAGGCAAACTTGCTGCAACCCACGCCAGGCCAGCT 
               
               
                 NO: 98) 
                 CGCCCACCGAAACCGCGGCTCCAGGANGCGNGGACTCAAACACCCCTGC 
               
               
                   
                 CTGGGCATCCTTGCCCNCGGTGGGAGAGGTGCCTGAAACTCAGAGGGGC 
               
               
                   
                 GGTGG [A/G] GTGCTATGATCTGCGCTTCTCCCTCTGGGTCTATCCAGGTG 
               
               
                   
                 TTTTGTACACGAAAAGATTTCAACACAGTGAAATAATCAATAACTTATA 
               
               
                   
                 AGGCATGTCTATACTTGCAAAGTGAAATGTGGAATTGAGATGGTTTGGA 
               
               
                   
                 GAGAAAAAGAGATTTTAAAAACAGACCCAAATAGTCCATNANAATAAA 
               
               
                   
                 AAACTTATACTTT 
               
               
                   
               
               
                 BIEC238830 
                 AGAACCCTAGAGGCCCAATGTTCTACAGNGGCTGTTTCCAAGAGGGAGG 
               
               
                 (SEQ ID 
                 AGGCTACAGCNACTGCCACACTGGATACACTCAGAGGAGAAGGAAAGG 
               
               
                 NO: 99) 
                 GGAGGACCCCGAATGCCTCTCATGTATAAAAGCGCGGGATGGCAGGAG 
               
               
                   
                 GCCTGTGTGGCCCAAGGAGCCAGAGGGAGCAAATTATGGGCAAAAGAA 
               
               
                   
                 TTGCAAC [T/C] CAAGGCTGGAAGAGAAGAGCTCTGCCCTCAGCTGTGTCC 
               
               
                   
                 CTGGCTCTCTGGCATCTGCTTCTAATGGGCAGGCGCCATTTTCNGAAGCA 
               
               
                   
                 TCAGGCACTGGAAAGAAGACCTCTGCTGCTGGGATTGAAAGGAAGTAAC 
               
               
                   
                 ANCCAGGAGGGGGAGATGCAAGGGCGAGGCTGGTCCCAGAAACAAGGC 
               
               
                   
                 CTATAATGCAGACA 
               
               
                   
               
               
                 BIEC263072 
                 TAGGCCAAGGCCCTTCATCATCAATTCGGGAATCAGCTGTTCTGCTCATT 
               
               
                 (SEQ ID 
                 CATAATCCAAGTGGATAAAAGCGATGTTAATTCCCTTTCTAGTTCTCTCA 
               
               
                 NO: 100) 
                 ACTAGAAAGTTACGGAAGGTGCGTTATTATCTAGGCAGAAACATGTCTC 
               
               
                   
                 CACTTAATATTGCGGGTTTGCTCCCTGGAAATGAAATGGTGAAGTGGGG 
               
               
                   
                 CA [A/G] TATCTACGTATGTGCCACTCTTCTCTCCTCTAAGAAGACTGNGA 
               
               
                   
                 GATCAGACTCAGGGTGAGAACCCTCACNCCAGCAAAGGCCAGGACACC 
               
               
                   
                 CACAAAACTGTCTCCGTGAATACATGTCTCAGAATCACCATTTTTGTTCC 
               
               
                   
                 ATCTTTCTCACCTCTACTTTTCTTTCTACAGTCTTCTCTTTAAGAACTAAG 
               
               
                   
                 TGTCCTG 
               
               
                   
               
               
                 BIEC281414 
                 AACTATTTCTGAGGAATAGCAATAGCAATAGTCTCATGAGTTCGGTACTT 
               
               
                 (SEQ ID 
                 ANAGGGCAGCCAATCTAAGTCAGAAGGGGATAGGATCTACCCTGGGTCT 
               
               
                 NO: 101) 
                 CCCTGGACCACGATTGGATCCCATGTCTCCTGAGTTTGTGTGGCTGTGCT 
               
               
                   
                 TCTTAAGCGCTCTGATCCTCGTGGATGCCAGCATGGTAGTCACTGTTCCT 
               
               
                   
                 T [C/A] CCAGACAAGGCCTCTCTTCTGTAGCAACCCTCTCCAAAGGCACAG 
               
               
                   
                 TTACCAACCTGCCTGCTCCTCCATTCAGGGTGGGCATATACAGGGGTGC 
               
               
                   
                 AGGTGGTTTCGCTGCAGCTGCCCTGTCCCCAACTGCCCCTCAGGTGGGTG 
               
               
                   
                 GGATGATCTATCTACCCTTGGCTTGTGTGGAAATCACATAGTAGCTGTGG 
               
               
                   
                 AAGCAT 
               
               
                   
               
               
                 BIEC293770 
                 AACATGGAATGATTTTTCTTTGGAATTTTGCATCTGTCTTCTTCTATCCCT 
               
               
                 (SEQ ID 
                 CCTACCACAATCATAACTCAAACCATAATTCATTTATGCCTGGGCAAATA 
               
               
                 NO: 102) 
                 CTATTGTCTACCAGCTGAATTCTCATCCACCTCCTTCTAAACTTTCACACT 
               
               
                   
                 GTTTCTTTGCTAAGTTTTTGTTTTCTTATTCTGTGCTTGAAAGCTTCA [C/T]   
               
               
                   
                 ACGATGTCCCATTATCAACAGCATAGAATCCAGTCTCTTTACTTAGCATC 
               
               
                   
                 AGATACCTTATAGTCCAGTCCTGACATTACTGGTTAATTTCATCTCTTCA 
               
               
                   
                 ATGTGTACATTGATATACACGCTTCTATTTATCATACTTCCAGCATGCCC 
               
               
                   
                 TATGATATTGGTTTTGCCTTACATTTGCTCTTCAGTCATGGAAATTTTTC 
               
               
                   
               
               
                 BIEC317564 
                 ggccactgctaaaaactgcccgctgttcctggccacacagcctgtcccaacatgttcacttgcttccttacaccagcaAA 
               
               
                 (SEQ ID 
                 CATTCTCTAGTGCGGTCTGCTAGGAAGATGGAGTCTTACATAGACAGAA 
               
               
                 NO: 103) 
                 CCTATCAAGAAAGTAGCATCCATCTCTTCACTATATCCTATTGGAGAGAA 
               
               
                   
                 GAAAGCCACCCAGCCATGACG [C/T] ACCACTTGATTCCACGGCTGGACG 
               
               
                   
                 GGAGGCTCACTGAGAGCGGCGGCGGAGAGGCTGAGCTGGACGCCAAGC 
               
               
                   
                 TCCGCTCGCTCGCAGCCTCCTTCCCTGCCCCAGCTCCTCTGCTTCCGGGC 
               
               
                   
                 CGCATCTGCAGGAGCACAAGCCCCGGGGCCGGTCCTGCTGCTCTTCCTG 
               
               
                   
                 AGCCTCCCCTGCTGGTGCTACCTTGGGCC 
               
               
                   
               
               
                 BIEC324581 
                 GACCCCCACCATACCAGGGAACATGAAGTGTAGGCAGCCCTGAGGCCCC 
               
               
                 (SEQ ID 
                 TGACTGNGGAGATGCGCTCATGGGTCCATTAAGCTGGGAGGTCACACTG 
               
               
                 NO: 104) 
                 CACCGCTGGCTGGCCAGGACCTGCGCACAGGGTTTCTTCTGACTACATAT 
               
               
                   
                 TTGTTTTTCAATTTGAATAGTTGTCCATGTTTAACCATCTCCAGATTTCTA 
               
               
                   
                 G [C/G] TTATCTTGAAAAATGAGCCCACACAGGATCCAGGATTTCACATGA 
               
               
                   
                 GCTGGGTCCCAACCCCTCCCAGTGGGGTGGGGTGGGCGTGCTTCAGGTG 
               
               
                   
                 CCGCCACTCCCTGCTTCTCCTGAGTGGCCCACATTACTAATTTAGGTGAT 
               
               
                   
                 TGCCTCGTCCCCGTGGGACCACGTGAGCAGCGCCTACTTCAATGTTCCTG 
               
               
                   
                 ACACCA 
               
               
                   
               
               
                 BIEC328312 
                 attagattcagagagaggcggagtaacttgctcacactagtaagcagagtttaaactgaggttaaatgaatagaaagcct 
               
               
                 (SEQ ID) 
                 gagttctttccactAAAAATAATTCTGTGTAACTCAAAATTTTGTTCTTATTTATTT 
               
               
                 NO: 105) 
                 ATTTAGCATAAAAAAGTTTCTTANGCCTGGTATTGGAACTTGGTACTCTG 
               
               
                   
                 ATAAAAGTATCAC [A/G] GCTTCTGAAAGCAGCCTAACCTTCCAAGAAAT 
               
               
                   
                 ACAGTGAATCAGAAGCCTGTTGTCTCTATCGACTCCCCAAGAAAGCTCT 
               
               
                   
                 AAATCTCACCTACATGCTTTCCAGAGTTGCTAAGTGCAGCCCTCCTTTCT 
               
               
                   
                 TAGCAAGGGATTCACCCAATCACTAGTCCATGCACTATTGAATTGCAGTT 
               
               
                   
                 TCAAATGCTGAGTGTAGAG 
               
               
                   
               
               
                 BIEC343880 
                 ATAACTAGAAAGGATGGGAAATGCCTTAGTGCTTACTATTTTTAAAAAG 
               
               
                 (SEQ ID 
                 CTACTTCCTCGATAGAAAGACTTTATTTACAAATAAATTGAAAAGGGTTT 
               
               
                 NO: 106) 
                 CTGAGGAAAACAACATATTACAAATACATTTTTGTTAACTTTNTTTNAAA 
               
               
                   
                 AAGCATCACCACAACATATGTCTACTCAAAGAGCCTTCAAAACTCCATT 
               
               
                   
                 TT [G/C] AGAAAAAGAGCAAAGAATCTTTATTTCCAAGCCAACAANCTAA 
               
               
                   
                 AGGGNCAGTTTGGTCANACGCGTAACGACAAGGAACCTTAGCTTCCTGA 
               
               
                   
                 TCCAGAGCAAACCCAGCAGTTCCTTTAAAGGTGACACAAGAAGGTAATG 
               
               
                   
                 AGGAGATCTAGAGAGTGGAAACGCAAGGCCTGCTGAAAGTCTGCGCTTG 
               
               
                   
                 CTTAAGCAGC 
               
               
                   
               
               
                 BIEC358651 
                 attcttttcatttctctgtaggtatttaccatatcccttcCTGGATTTGATCATTATAAATCTTATATC 
               
               
                 (SEQ ID 
                 ATGGTTATTTTAATTGATTCTGAGCTTTCTCCTGTCCTTACtttatttatttatttatttatt 
               
               
                 NO: 107) 
                 tattGAAGTACTTCATCCAGAAACAGCATCCTACACACGGGCTGCCATTGT 
               
               
                   
                 CTGCTACTAGAGTTGCT [T/G] TAATTTAACTCTCCCCCTTCCCTGTCAAAA 
               
               
                   
                 TTACATCCTAAGTATGCTCTGGGCTCAAACCAGAATGATCTTAATGTGGA 
               
               
                   
                 TGCAATTGTATTTGAACCAAGGGTCAGCTTAGAGGCAGCTGGCAAAATG 
               
               
                   
                 TTCAACAGACAGGACCAAAAGGAAGAATTGTAACAGAAAATGTGATTCT 
               
               
                   
                 GTCTGGAGCAAAGTGAGAAAGT 
               
               
                   
               
               
                 BIEC425746 
                 CATAGGCTGAGTGGCAGAGGACCCTTACGAATCCCCCAATTCTTTGATA 
               
               
                 (SEQ ID 
                 CCTAAGGCAACTGTGTACAAGCTGATAATATAAATCTTGGGAAATAATA 
               
               
                 NO: 108) 
                 ACCGGAGAAATTCTAGGGCGCTCAGTTCTGGAAATAACTTTTGTAGAGC 
               
               
                   
                 TCTGGATCTAAGGGCCTACCTTTCTTGGACAGCCTACACGGTCCCATGCA 
               
               
                   
                 AGC [A/G] TACCACTGCTGGGATGGTCTAGTTCAAAGGAAGAAAACACCT 
               
               
                   
                 TCCCTGCCTTTCTGCTTCAATTTGCCCTAACCATTTTCTGATTTGAGAAAA 
               
               
                   
                 GCAACATAGCAAGGGGTACTAACACTTCTGTACTAATCGGCCAGGGTGA 
               
               
                   
                 GGAGTGAACAATTAGAGTTGCTCTTTTAACTCTAATGGTTGTCAAGGCA 
               
               
                   
                 GGGAGATGA 
               
               
                   
               
               
                 BIEC441654 
                 CTGTGTGGATTTATAATATTTCACTGTACTTTCCACTGACATTAATTGAA 
               
               
                 (SEQ ID 
                 TAAAATAATCTTATAGAGAGGTTTGGCTTCAGTTTAGTCACAATCTGATG 
               
               
                 NO: 109) 
                 ATTAAGTTTGATTTAATGCTATTTATTATTCTGTTTAAAAGGAGTCTTCA 
               
               
                   
                 GAATTGTGTCATCTGGAGTCCAATGGGCTAGTTATCTGGTATGCTTATCC 
               
               
                   
                   [G/A] TTCTCCAATTTTGATTATTTTCTTCAAATCATTTTAAAATGTTATTTT 
               
               
                   
                 TCATGAACATTTCCTAACACTGCACTTGCATTTTCCATTTCACACCTCTCT 
               
               
                   
                 AATCTATGTACAGTAAGGCTGGTATGACANCCTATTTAGTNATCCACAG 
               
               
                   
                 TGTGGTNTTNATGAAGCTCATGTTACAGTTCCATTCCCTACACAGAACCT 
               
               
                   
                 AA 
               
               
                   
               
               
                 BIEC455646 
                 AACAAGACCTTGATCATGTGCCACTGCGAGAGGAACTCGACGCGCCAGC 
               
               
                 (SEQ ID 
                 TCATGCGGCCGTGGCCTGAGTTGGGAAATGGCCAAGAGGAGTGAGCTGG 
               
               
                 NO: 110) 
                 AGCTGTGCTCATGGCAGTAGTGTCCGGTGGGATTCTGGTCAAGTCAGCT 
               
               
                   
                 GGAGGTTCCGCCGGCAGGGTGGAGGCCAGAGGTATAGGGGTGTGGGTC 
               
               
                   
                 AGAGG [C/A] CCCAGGGAATGTGCTGGAGTCATGGTGAGAGTAGCACCTG 
               
               
                   
                 CCACAGGCTTTCCGCGTGGCTGCTGGGCTCTGGCGTATGCTGTGTTGCTC 
               
               
                   
                 GCCTCCCCAGGGTAGCCTTGACATGATGAGCGATGGGAGCATCCCTTGG 
               
               
                   
                 AAGACAGCCTCCCTGGGGAGCTGCGGGAGCCAGGAGGCACAAGCTGCA 
               
               
                   
                 GCCGGGAGCAGAG 
               
               
                   
               
               
                 BIEC476263 
                 TATCCTCATCTAAAGCCAATGATAAGGNTTTTCAACATGTTGCTAAGAA 
               
               
                 (SEQ ID 
                 GAAGTCTATTGCAATAGATTGTTCCTTTCTAATTCTTTGTCACCTGGTTTC 
               
               
                 NO: 111) 
                 TTTTCTCCTTGAACATGGGTGGGAAAGATGGGCCACAGGTTTTTCTGCCC 
               
               
                   
                 ACCTGGAAGGAATGACTCTACACCACCCCCTCTAGCAGGACTAGCACTG 
               
               
                   
                 A [G/A] CTCTGACAAGATAGTCTTTGTCCAGGGTTGGTGATCTTGGGACCT 
               
               
                   
                 GAGGCAATGAGATACAGGACAAGAAGGGATCATTGGAAAGGTCTAACA 
               
               
                   
                 AAGGAAAGTGGGGAGCCACTTTCTTGGTATATTTGAGTCACAAGGCTTT 
               
               
                   
                 GGATGCTCACCTTGCTATTTAATTTTTACAATTCCATTTTGTGATAATCAA 
               
               
                   
                 NATCTGT 
               
               
                   
               
               
                 BIEC480445 
                 CAGGCAAGAAGCGTCTCAAGGGTTGAAAAAAATACAATCTGCCAATGTA 
               
               
                 (SEQ ID 
                 GACTTTCAAACAGATCCCTTTTTTCTTTGATTAAAAAAAAAAATGGAGTT 
               
               
                 NO: 112) 
                 ATTCCAAGATAGAAGTTACTGCAAGATAGAAGGTTGACAGTACTAAATG 
               
               
                   
                 ACAGTCAAAAAACATATAACCTGAATAAGTAAAAAGAAATTAAAATGA 
               
               
                   
                 AGTC [G/A] TTGGTATTTTTATGAATTTATGAAGTCAGCATGGTCTGTGGG 
               
               
                   
                 TATAGATGCAGCTAAAACCTATGTACTCAAGTTTAAATTGCAGGTTGATT 
               
               
                   
                 TTTCTACCCACACATATTTAAGTCAGTTGCTTTATTCTCATTTGGAGTTTA 
               
               
                   
                 GCTCCCAACCTTGCACAAGATCTTGAACTTAATCTCATGTATTCTAGAAT 
               
               
                   
                 TCAAGAT 
               
               
                   
               
               
                 BIEC500415 
                 TGCACATCCTGATAGCAGCAAAGACGAAAGTGTGNGAGGGGAAGGGAT 
               
               
                 (SEQ ID 
                 TNATCCCGAGGCAGCCAGCTCATCATCNGCAAAACTGGGATAGGAAAA 
               
               
                 NO: 113) 
                 AAGCTCGGGTCCTTCTCCCACAACTTAAGCTCGCATCTCCTAATTTTCAT 
               
               
                   
                 AATTGAGTGATTTTCCCACTCTTTCCATCATTTTGGCTGGATCCTGCTGA 
               
               
                   
                 GAAA [G/A] ATGGCTTTTTTTCAGAGCTGGAATAAAGACTCTTCAAGTTGA 
               
               
                   
                 TATTGGGTTTAAGCCACAGATGCTAAGATGTCATCAAGTTCAAAGTCGG 
               
               
                   
                 AATCTTCTAGAATCTTTGCCTGCAGACAGAGATGCTGAGCCAGCTGGCA 
               
               
                   
                 GACGTGGTGGTGAGGACATGCAGAGCTCCCATACACTCCACTTGTCCAT 
               
               
                   
                 GGAATTGTACG 
               
               
                   
               
               
                 BIEC514026 
                 TTTTGTGATAAAGGATTTCTTTGCATTTTTTCCTCTAGTCAAGTAAATTGC 
               
               
                 (SEQ ID 
                 TTGTGGGTTCTTCCTAAGAAAAATAATCCCTCTGGTGCTGCTTTTAATTT 
               
               
                 NO: 114) 
                 GATCAGGTTTAAAATGTTTTCAGAAGAGTTAAGCTTCCTTTACATTGGTG 
               
               
                   
                 TCTGGTGTGGTCAGATGGAGGAATAGCTTTGGAATGAACTAGATTTTTT 
               
               
                   
                   [A/C] GTGATGCACCGTTTGACCTTCCCACAGAAGGTTCAGTACAAGGAAT 
               
               
                   
                 CAGTCAAAACAACAGCACCATTTTCACTTGACCTCGAGACATGTGGTGT 
               
               
                   
                 ATACCCTTTACCCCGACAGATAGAACTTCCTAAGCATATTTTTCTTTGAC 
               
               
                   
                 TCATGTTGTAAGAGTTTATGTTTCTTATGATATATATCCATTGTGTCCAAC 
               
               
                   
                 TGTC 
               
               
                   
               
               
                 BIEC526317 
                 AAATTCTTTTTGAATGTTTACATTACTTTTCTGGTTAATAGTTTTAAAATT 
               
               
                 (SEQ ID 
                 CTGTGAAGGAGCATCTCTGAATTTATCTGAAATTTATAGATACTTTCCTT 
               
               
                 NO: 115) 
                 ATTCAAACAAAAACAAAACCACAACACAAACGCAAGGAAAAACAAGGG 
               
               
                   
                 TCCAATAAAGTGGAAACTTCTGTTATGGTCTAACTTTTGGTCAGCAGTAT 
               
               
                   
                 G [C/T] AAGCATAATTTTGGTTCAGGACTAACGCTAACGAGAGGCAAAGC 
               
               
                   
                 TGAGGCTACGGCTACGGGATGATGGCTGAGGCTCATATTGTATTACTGG 
               
               
                   
                 AGGGGCCCAGGGGGAAGTTAAAATGAGACACTAGCTCCTGTGCATCAG 
               
               
                   
                 GACCGTCAGCTCTAGAGGTGTCAGGGGCCCCTGAGTTGGAGCAGTGAGG 
               
               
                   
                 AATCCCCTCC 
               
               
                   
               
               
                 BIEC542390 
                 CCCCCAGCCCTCTGCTGGGTTCCTACCAGGCTCCAGCATATTGACCCCCT 
               
               
                 (SEQ ID 
                 GACTTCATGCCTCTGTTCAGACCAGGGTAGATGAACTGACAGCCGCCCA 
               
               
                 NO: 116) 
                 AGGAGCTGCCCCTTCCCCCCACCACCACCTAACCATGTCCCGCAGAGGA 
               
               
                   
                 CACGCAAATAAAAGGGCCCTCTGAATGGACTTCAAATGCAAAGACAAAT 
               
               
                   
                 TCT [C/A] AAAAGGCTGTGCATACAAAATGCACACATTGGTTGCCAGAGAT 
               
               
                   
                 ACTAACGTTCATTAGTATTTATTAGAAATCGTGACACTGACACTTAGTTC 
               
               
                   
                 GAGGGTCAGTCTCCGTGAAGGCGGCTGGCCGTGGCTGGGTGTGGCCAGC 
               
               
                   
                 CAGCCCCCCTACTCCTCTCCTGGANGGAGATGGCCTGTGGGGAGCTGTG 
               
               
                   
                 CCCCCAAGC 
               
               
                   
               
               
                 BIEC544278 
                 CCCACAGGACTCGGCTTCTAGGCGGCAGGGAGTGACTCCAGGACCAGAG 
               
               
                 (SEQ ID 
                 AGCAGGCAGCAGGAACCCTGAGGGACTGCAGGAAGCCAGGCTGCCCAC 
               
               
                 NO: 117) 
                 TCACTCAGTGGTTTGCAGGCAGAGGGAGCAGCTAGAAGCCCAGGAGAC 
               
               
                   
                 CCTTGTCCACCAGCCGCCTCCTGGGCCCAACAGCCGCCCGCGGGCAGGC 
               
               
                   
                 CCGGTG [G/A] GAATGCTCATCCGACCTGCGAAGGTCTCCATACTGCCAGT 
               
               
                   
                 CTGGGCAGACTATGCGGGGCTGACAGTTGCCCCCAGATGTTTTACAGCA 
               
               
                   
                 GCCGTGAAAGGGCCTCGAACTCCACAGATGGCGAGCGACTCGCAGCCAC 
               
               
                   
                 TGGTTGTGGGTGTTCCTTGCTAACATCTGcacacacacacatgcacacgtgcacatgcatgc 
               
               
                   
               
               
                 BIEC555903 
                 AAAAGGTCACTTTCCAAACTGCTTTTGCTCCCAGGCTCTGCTCTGAATAA 
               
               
                 (SEQ ID 
                 TTCAAGTCATCCTCAGTAAGAGCAGCAGGCTTTGGGGTGATCTCCAGCC 
               
               
                 NO: 118) 
                 TGTTTGACAGGAACGGTGCTGACTTAAGCTAACAAGAGGTCATTGTCTG 
               
               
                   
                 AGCAGAAGAATGGCATCCTAGCTTCTTGATGAGCAGAACCAGGCATGGG 
               
               
                   
                 AAC [G/A] TAAAACAAGACCAACTGACTCATTGTAACTGAACCAGAGGCA 
               
               
                   
                 GCCAAGTGCCTTCCCAAATTCCCTTCTTAGCAGGACCAAGCCCTTGAGG 
               
               
                   
                 AGGAGGAGGCATTTATTGGGGATTGCTACAAATTCGGCTTTACTGCCAC 
               
               
                   
                 CGCCTTTATAACCACAATATAAAGTAACTCCCACAACACAGTGAAGATA 
               
               
                   
                 AGCTCTTTAAA 
               
               
                   
               
               
                 BIEC562394 
                 ACTGTGTGAGCAGCTGACCATCCTCTCTGGGTGGAGGTAGAGTGTAGAC 
               
               
                 (SEQ ID 
                 AGACAGATAAAAGGTTTTGTCTGGATGAAACCTTCATCATCTGTGACCT 
               
               
                 NO: 119) 
                 GCTTGAAATGTAGGAACTGCTACTCGGCAGTGAGGACACCCACCAGGCC 
               
               
                   
                 CCTGTGACCTCCANGGGTGTGTTACCAGCACAGGCAGAGCACTCACAGT 
               
               
                   
                 GATG [G/T] TCCTCTTCACAGAGGTGAGTCGGGGGAGATTCTTGGGCCACA 
               
               
                   
                 TCTCTCTACAGTCTAGNCTTTGTAGTGTGTCCTGCATGTGGTGGTGTGGG 
               
               
                   
                 GACTGATTCTGGTGGGGACCCCTTGCTGCATTAGGTCTCATTCACACCCT 
               
               
                   
                 TATTTCAGTTTGTACTGTGGCTCCCATGCCTGGTTACCTGCAGTGCAGTG 
               
               
                   
                 GTGACTCT 
               
               
                   
               
               
                 BIEC580675 
                 AATTACAATTTTAAGGAAAAAATCTATCTCCTTGTCATCACATTTCCCGA 
               
               
                 (SEQ ID 
                 CCTTCCTTTGAGAGAGATTCTAGAGTCCATTATTTCTGTGGAGATTGCTG 
               
               
                 NO: 120) 
                 AAAATATTTTTACAACTCTCTCCAATATATTTCTACTATTAAATATCTCCA 
               
               
                   
                 TCTTATTTTACTAATTCATGTTTGTCTAAATTCTGAGGTTTTACAGGCT [T/   
               
               
                   
                 C]TTTGGTATTGCAATTCTCTTATCCAGCTCTCCATCTATGGAACATTAGG 
               
               
                   
                 AAGTCTCTGAGGCCGGTCTGAATCTCATAATTTGGTAGTGAATCAGACC 
               
               
                   
                 AACGATTTAAGAGGTGGTTTTCAGAGAAAGTCCAATTGTGTTTNAAAAC 
               
               
                   
                 ATCTGCAAAGTACGTTTTTCCCCTCAGCATCTAGAAGGCACACATGAAG 
               
               
                   
                 TTGA 
               
               
                   
               
               
                 BIEC590604 
                 TCTGTGGATTTTCAGTCTATATCCTGCTTCCTTTATGGGGCTCTGGCCTAA 
               
               
                 (SEQ ID 
                 GGGTGTGGTCACATGGTCACTCATGGCACCAGGCCAGCTTGGAGGGTGT 
               
               
                 NO: 121) 
                 GCTCTGCCCTTCTCTCTTTCCGTGACATTGGCATCTCATTGAGTCCTCCGT 
               
               
                   
                 CTCCTTTCTCTGCTCTCTTTCCATTTCATTCCCTCCTCAAATCTTTGTT [T/G]   
               
               
                   
                 TTCCCATTGAGGAGAGAAAGCCTCGCTTTCCAGTGGGGCTTGAGTATTT 
               
               
                   
                 CCTACTCAGGTTGATGCGCCTCTTTTGGGAATATAAATTTTGCTTTCCTTT 
               
               
                   
                 CAGTTTTCTCCTCTGTTTTCAGTTCAAGACAATCTTGTGTACATGGCCAA 
               
               
                   
                 ATAAAACAGCGTGTTGTCAAATCCCGGGACAGGATGGTGATGGTTATTGA 
               
               
                   
               
               
                 BIEC600682 
                 CCACATGCTCTTTCCCACGCTGTCCTGAATTTATATACCATTATGTAATTT 
               
               
                 (SEQ ID 
                 TATTGAGAAAGATATAGGTATCAATACCTTGAGCAGTGAAATTAGCTTC 
               
               
                 NO: 122) 
                 TTCACTCTCTCATGCTTAAGCTAACGTTACAATGAGTCAGCATGCCCATC 
               
               
                   
                 ATCCACAGTAACTCACNGAGGACGAACCAGTCTCGACTGGCACATGAGA 
               
               
                   
                 T [G/T] ATGAATGGACTAGTGTAGGATTAAAAGTTTGCCTGATATATTAAA 
               
               
                   
                 TGATGAAATCGTGAAAAATGCATACCTGGTAGGAAAAACTTAATGGGTA 
               
               
                   
                 GGATGCATGATGAACTCAATATGAGGGAAGTAGGGGCCACTGCAGAAT 
               
               
                   
                 ATGGAACTGAGGGGTGATTCTTAGTTATGGGGAAAACCCAAAGGTGTGC 
               
               
                   
                 CTTTCCTCA 
               
               
                   
               
               
                 BIEC622581 
                 ACCACCTTTCTGAGTCCATGCCACTGGTGGAGGCCCTGCCAGCTGCCAG 
               
               
                 (SEQ ID 
                 ACCGCCGGGCAGGGTGCAGTCCAAGGGGCTCCAGCTGTGGTCTCTGCCC 
               
               
                 NO: 123) 
                 TCAGCTCAGTGGAGCTGAGGAAACATGCCATACAGCCAGACCTTACGTG 
               
               
                   
                 GGGAGCAGCCCTGGCCAAGAATGAGATAAACGGGGGGTCGGCAAGGTC 
               
               
                   
                 CCAGC [A/G] AGGGCATCTTGCACACCTGGATGGCCAGGGACCACAAGGG 
               
               
                   
                 AGCCAAGTCATTGGGCTGAAGGGCAGGTCAGAAGGCAGCCGAGCCACT 
               
               
                   
                 CACCAGCATCCTGGAAGCCACACAGGTGCTCCATNCGCTGGTCAACACA 
               
               
                   
                 GCATGGGGTGGGCACCTCCTGCAGGGNCAtgggggcagacccaggccccagtgacttcac 
               
               
                   
                 cag 
               
               
                   
               
               
                 BIEC633730 
                 TTCCATGCGACCAGGCTAAGGACACGAGCAACTCTCAGTAGCAAATATG 
               
               
                 (SEQ ID 
                 AAAATCCAAGCAAAAGAAAGAAAGACTACATTCAGCTCTTGTAAATCTC 
               
               
                 NO: 124) 
                 AGTCTCGCTCANCGCCAGGTGGACATGACAGTTCATTGNCGCGACCGTG 
               
               
                   
                 GATGGCGGGTGTGTCCGGGTGCCTATGCTCTATAAAATACAGGAGGCAC 
               
               
                   
                 CACA [T/C] GTCTGAGATTGTCGAATGTCTAGTGCTAATAAACGTCACACC 
               
               
                   
                 CTGCAGCTTCTGAGGAGAAAGAAACTGTCCCAGTCCACGGCTGGCTCTG 
               
               
                   
                 CTAATCAAGGCCAGCTGCTTGGGCCTGTGTCCAGAGAGTCAGGNAAGGG 
               
               
                   
                 TGGCGGGGAGGGAAGAGGAGAAACAGGGGCCCCATCACCGCCACGGGG 
               
               
                   
                 GGACTCCCCTGT 
               
               
                   
               
               
                 BIEC637205 
                 AAATGGAATAACAAAACAAAGAAAACAACAACTGTGTTTGTCGTTGTAG 
               
               
                 (SEQ ID 
                 CTGAGCAGGCATGGCCTTTTCATAGCGCATCTGAAAGTGGGAGAGAGTG 
               
               
                 NO: 125) 
                 TAGGATTTGTCCTGAGCATTGTTCCCGGGATTTGCCGTCACAGAACAATT 
               
               
                   
                 CTGATTTCAGTGGGAGGGTAGGGCCAAGCAAGACTGCTTTTGCTCTCAG 
               
               
                   
                 CCT [A/G] TTTAAAGAAAATGGTGAGCTTGCCTAGGAAATACCCAGTGTTC 
               
               
                   
                 TGCAGGCCCACTGTGGCTTGTTGCATTAACCACCCAAACAAAAAAATGT 
               
               
                   
                 TACTGTGCATCCTTTCTTAAAGAAATGGAACAATGAGACTGATGTTGGCT 
               
               
                   
                 TCTTGAATGGAAATCTAGGGGCATAAGCCACCCATTCTCTACAAAACAA 
               
               
                   
                 AACAACATG 
               
               
                   
               
               
                 BIEC687178 
                 AATCCTCCACACTAAAATCTAAGTCTAAAGACTGGAAGCCCTGATTTTTC 
               
               
                 (SEQ ID 
                 TGCCAGTTAGTCACTCATTTATTTATtcaataaatatctatcaagtatttactatgtgctaggtcctat 
               
               
                 NO: 126) 
                 tttaggtgttaggaaaacaaaaaTGATTAAGACACAAAATTTCTTGAGCTCTTCCCATA 
               
               
                   
                 TGTCAGACACTCTCAAAAGGAA [T/G] TCATAACCCAGTGGAGGCATAGA 
               
               
                   
                 AGGCAAATTGCCCAAATGACTGTTGTACAGTGTCCTATAATACAGGCTG 
               
               
                   
                 GAAGAGGGTTAAAGGAGAGATCAATGAGATGAGAGAGATGTCGTACAA 
               
               
                   
                 GCCCTATAAGACAGGAGTCAAAAAGGACCTCTAGAAAGAGCAGCAGCC 
               
               
                   
                 TCATTTACAACCCACTATGGTCCAGGGTGCTA 
               
               
                   
               
               
                 BIEC706272 
                 TGATGGTGCNAAGATTTCATGATTGCTTTTCATCCTTGCTGGAAGGATCA 
               
               
                 (SEQ ID 
                 ATGAGACTGGGGATATCTCTGGAATCTGATTAAAATTTCTGGCAGGATC 
               
               
                 NO: 127) 
                 GAGTTGTGGGCACAGGAGAGAAGGTCACCATCTCTTTATCAACCCACCT 
               
               
                   
                 CAGAGTCCTACCTCATGATCTAGGTCCTAAAAGGAAAGGCAGCACCACA 
               
               
                   
                 GGC [A/G] AAAAATATTCTAACAATGGAAAACTGCTTCCACAGTCAGCTGT 
               
               
                   
                 AGGAGTCAGCAGAGCCATGCTCATTTTGTGTAATCTGAAGGTCTTAGAA 
               
               
                   
                 AGAAAGACGAGTGACAGAAATGTGATCCCACACCTCTACTCTCATCTCC 
               
               
                   
                 TGCCAGGCTTTCTCCCATGACAGCTCCATTTCTCTAGGCCAAGCAATTCT 
               
               
                   
                 TTCCTTCTA 
               
               
                   
               
               
                 BIEC942271 
                 TAAGGGCAAGAACTCTCTAGATTTCCCAAAATGAGGCATAAGCAGGAAC 
               
               
                 (SEQ ID 
                 CCTTGGTGGTAAGGAAACCCCAAAGTTTGCTTTCAACCTGAGTATGCTA 
               
               
                 NO: 128) 
                 AACAAATCCTGGATAATTTGAACTTTTGCACCGGGTATCATGGCAAGAA 
               
               
                   
                 TTTAAAACCTATGACCTGTTCATGATGGGTAATCAAATGGGAAACTCCCT 
               
               
                   
                 GCA [C/T] TAAAATAGAATTGCAAACTGTGAATGTGTAATAAACTTTGCTG 
               
               
                   
                 TAGGGAAGGGAGACAGAGGGAAAATTACCCAACTCATTTTAGGCACTTG 
               
               
                   
                 GTAGAAGTTCAAAAACAAACAAACAAATCAAAAAAACAAAAGAAAAAA 
               
               
                   
                 GTAAAACCTCATCTGATAATTCTGGAAGGAAATATCAGACTCAAACAGG 
               
               
                   
                 CTCTGGTTCCA 
               
               
                   
               
             
          
         
       
     
         [0079]    In further embodiments, the present invention provides a panel comprising a plurality of assay compositions, wherein each assay composition is capable of identifying at least one of the nucleotide markers as set forth in Table 5 below: 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 DOG SNP PANEL SEQUENCES (SET #1) 
               
             
          
           
               
                 SNP ID 
                 Chr 
                 Position 
                 G 
                 A 
                 Discovery Breed 
               
               
                   
               
             
          
           
               
                 BICFG630J1290 
                 1 
                 9088016 
                 A 
                 T 
                 Alaskan Malamute, Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J5593 
                 1 
                 19444782 
                 A 
                 G 
                 Beagle, Boxer, Poodle, Rottweiler 
               
               
                 BICFG630J227421 
                 12 
                 57771412 
                 C 
                 T 
                 Bedlington Terrier, Boxer, Poodle, Rottweiler 
               
               
                 BICFG630J232150 
                 12 
                 64596878 
                 T 
                 G 
                 Beagle, Boxer, Poodle 
               
               
                 BICFG630J235932 
                 13 
                 12459211 
                 A 
                 G 
                 Alaskan Malamute, Boxer, Italian Greyhound, Poodle 
               
               
                 BICFG630J255886 
                 13 
                 43403946 
                 T 
                 G 
                 Beagle, Boxer, India Gray Wolf, Italian Greyhound, 
               
               
                   
                   
                   
                   
                   
                 Poodle, Portuguese Water Dog 
               
               
                 BICFG630J265884 
                 14 
                 12884975 
                 A 
                 G 
                 Boxer, German Shepherd, Poodle, Rottweiler 
               
               
                 BICFG630J275606 
                 14 
                 44515618 
                 C 
                 A 
                 Boxer, German Shepherd, Labrador Retriever, Poodle 
               
               
                 BICFG630J278829 
                 15 
                 16039028 
                 T 
                 G 
                 Alaska Gray Wolf, Bedlington Terrier, Boxer, 
               
               
                   
                   
                   
                   
                   
                 Labrador Retriever, Poodle 
               
               
                 BICFG630J282369 
                 15 
                 24063852 
                 T 
                 G 
                 Alaskan Malamute, Boxer, English Shepherd, 
               
               
                   
                   
                   
                   
                   
                 German Shepherd, Poodle 
               
               
                 BICFG630J304928 
                 16 
                 3886095 
                 G 
                 C 
                 Beagle, Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J319569 
                 16 
                 43009172 
                 T 
                 G 
                 Bedlington Terrier, Boxer, China Gray Wolf, 
               
               
                   
                   
                   
                   
                   
                 Italian Greyhound, Labrador Retriever, Poodle, 
               
               
                   
                   
                   
                   
                   
                 Rottweiler 
               
               
                 BICFG630J331636 
                 17 
                 14160564 
                 G 
                 A 
                 Bedlington Terrier, Boxer, English Shepherd, Poodle 
               
               
                 BICFG630J346559 
                 17 
                 48612703 
                 C 
                 T 
                 Boxer, Labrador Retriever, Poodle, Rottweiler 
               
               
                 BICFG630J356853 
                 18 
                 22596913 
                 T 
                 C 
                 Beagle, Boxer, Poodle, Rottweiler 
               
               
                 BICFG630J358084 
                 18 
                 44102666 
                 A 
                 T 
                 Bedlington Terrier, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J373954 
                 19 
                 21194807 
                 C 
                 T 
                 Beagle, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J391832 
                 19 
                 56281961 
                 T 
                 C 
                 Bedlington Terrier, Boxer, Italian Greyhound, Poodle 
               
               
                 BICFG630J402866 
                 20 
                 58705091 
                 G 
                 A 
                 Boxer, German Shepherd, Labrador Retriever, Poodle 
               
               
                 BICFG630J399661 
                 20 
                 39886765 
                 C 
                 G 
                 Alaskan Malamute, Bedlington Terrier, Boxer, 
               
               
                   
                   
                   
                   
                   
                 Italian Greyhound, Poodle 
               
               
                 BICFG630J414309 
                 21 
                 28639360 
                 A 
                 G 
                 Alaskan Malamute, Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J421119 
                 21 
                 49794475 
                 T 
                 G 
                 Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J431948 
                 22 
                 21464933 
                 C 
                 T 
                 Beagle, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J425382 
                 22 
                 6210670 
                 T 
                 C 
                 Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J457850 
                 23 
                 13946934 
                 G 
                 T 
                 Beagle, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J473226 
                 23 
                 36806100 
                 A 
                 G 
                 Beagle, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J484553 
                 24 
                 8851728 
                 A 
                 C 
                 Boxer, German Shepherd, Labrador Retriever 
               
               
                 BICFG630J497958 
                 24 
                 29602886 
                 T 
                 C 
                 Bedlington Terrier, Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J503647 
                 25 
                 3274907 
                 A 
                 G 
                 Bedlington Terrier, Boxer, German Shepherd, Poodle, 
               
               
                   
                   
                   
                   
                   
                 Rottweiler 
               
               
                 BICFG630J525153 
                 25 
                 52605143 
                 A 
                 G 
                 Beagle, Bedlington Terrier, Boxer, Poodle 
               
               
                 BICFG630J533364 
                 26 
                 21482093 
                 A 
                 G 
                 Boxer, German Shepherd, Poodle, Rottweiler 
               
               
                 BICFG630J537466 
                 26 
                 28425454 
                 G 
                 A 
                 Alaskan Malamute, Beagle, Boxer, Poodle, Rottweiler 
               
               
                 BICFG630J548189 
                 27 
                 5814598 
                 A 
                 G 
                 Boxer, German Shepherd, Poodle, Rottweiler 
               
               
                 BICFG630J553154 
                 27 
                 16146331 
                 G 
                 C 
                 Bedlington Terrier, Boxer, German Shepherd, 
               
               
                   
                   
                   
                   
                   
                 Poodle, Rottweiler 
               
               
                 BICFG630J566667 
                 28 
                 11501579 
                 T 
                 C 
                 Bedlington Terrier, Boxer, English Shepherd, 
               
               
                   
                   
                   
                   
                   
                 Poodle 
               
               
                 BICFG630J573029 
                 28 
                 23791787 
                 T 
                 C 
                 Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J585149 
                 29 
                 15036709 
                 G 
                 C 
                 Bedlington Terrier, Boxer, Poodle 
               
               
                 BICFG630J597522 
                 29 
                 41369057 
                 T 
                 C 
                 Bedlington Terrier, Boxer, Italian Greyhound, Poodle 
               
               
                 BICFG630J608671 
                 30 
                 28455073 
                 G 
                 A 
                 Alaskan Malamute, Beagle, Boxer, Poodle, Rottweiler 
               
               
                 BICFG630J613547 
                 30 
                 39085959 
                 C 
                 T 
                 Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J630348 
                 31 
                 30276777 
                 A 
                 G 
                 Beagle, Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J635046 
                 31 
                 41099916 
                 G 
                 A 
                 Boxer, German Shepherd, Labrador Retriever, Poodle 
               
               
                 BICFG630J638804 
                 32 
                 14056351 
                 G 
                 A 
                 Boxer, German Shepherd, Poodle, Rottweiler 
               
               
                 BICFG630J636447 
                 32 
                 7803442 
                 G 
                 A 
                 Beagle, Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J654194 
                 33 
                 11445001 
                 A 
                 G 
                 Beagle, Boxer, Poodle, Portuguese Water Dog 
               
               
                 BICFG630J660369 
                 33 
                 26075493 
                 C 
                 T 
                 Alaskan Malamute, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J667882 
                 34 
                 30670918 
                 G 
                 C 
                 Bedlington Terrier, Boxer, German Shepherd, Poodle, 
               
               
                   
                   
                   
                   
                   
                 Portuguese Water Dog 
               
               
                 BICFG630J676160 
                 34 
                 40730781 
                 T 
                 C 
                 Boxer, English Shepherd, Poodle 
               
               
                 BICFG630J689381 
                 35 
                 25937791 
                 A 
                 C 
                 Bedlington Terrier, Boxer, English Shepherd, Poodle 
               
               
                 BICFG630J678332 
                 35 
                 6882284 
                 A 
                 T 
                 Alaska Gray Wolf, Alaskan Malamute, Boxer, Poodle 
               
               
                 BICFG630J693521 
                 36 
                 7667844 
                 A 
                 C 
                 Beagle, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J695147 
                 36 
                 11554366 
                 T 
                 C 
                 Beagle, Boxer, Labrador Retriever, Poodle 
               
               
                 BICFG630J707814 
                 37 
                 12867303 
                 G 
                 T 
                 Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J715531 
                 37 
                 33363432 
                 T 
                 C 
                 Beagle, Bedlington Terrier, Boxer, Poodle 
               
               
                 BICFG630J719405 
                 38 
                 19640071 
                 A 
                 G 
                 Alaskan Malamute, Boxer, German Shepherd, Poodle 
               
               
                 BICFG630J724770 
                 38 
                 26352306 
                 A 
                 G 
                 Beagle, Boxer, Poodle, Rottweiler 
               
               
                 BICFG630J729876 
                 X 
                 4043645 
                 T 
                 C 
                 Boxer, German Shepherd, Labrador Retriever, Poodle, 
               
               
                   
                   
                   
                   
                   
                 Portuguese Water Dog 
               
               
                 BICFG630J749105 
                 X 
                 98054740 
                 A 
                 G 
                 Beagle, Bedlington Terrier, Boxer, Poodle 
               
               
                 BICFG630J745699 
                 X 
                 88286773 
                 A 
                 T 
                 Beagle, Boxer, Italian Greyhound, Poodle 
               
               
                   
               
               
                 G = genomic allele; A = alternative allele; O = Other SNP within 30 bp of genomic/alternate allele: P = percent repeat. 
               
             
          
         
       
     
         [0080]    The nucleic acid sequences of the markers of Table 5 are provided in Table 6 below, where the position of the polymorphic site (e.g., the single nucleotide polymorphism (SNP), insertion and/or deletion) is bracketed and indicated in bold: 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 6 
               
               
                   
               
               
                 DOG SNP PANEL (SET #1) NUCLEOTIDE MARKER SEQUENCES 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 BICFG630J1290 
                 GATTAGACCTTTAATGTTACAGCAAATATGGTTTATGATTCTTTT 
                   
               
               
                 (SEQ ID NO: 129) 
                 TTAAAATTTCAAATAAAACTTTATGTTGAGAGCTATGACTGCAG 
               
               
                   
                 TTCTTTCTCTTGTCCTCCCTTACCTAATGCCCCAAATTACTTTGGT 
               
               
                   
                 TGTCTTCTACTGAAGTTTTTATTTCTTAAAAATCCGCAACATATA 
               
               
                   
                 GGTCTAGGTGTTGTCTCAGA [A/T] GCCATGTAGGATTTAAACATC 
               
               
                   
                 CCAACAGAGTGAAATGCTATTTCAGGAAATACGGTGCACGCTTG 
               
               
                   
                 CCACCTAGTGGTGAGTGTGGAAACAAGTGAGGATTTCAAAGCA 
               
               
                   
                 ATCCCAAAGAACGTGAATTCNNAGAAANACTAAGTTCACTAGTT 
               
               
                   
                 ATNTTCAAATATAGTAAGGTATAAGTGTTATGTGAAAACTATTA 
               
               
                   
                 TTTT 
               
               
                   
               
               
                 BICFG630J5593 
                 ACTCAGCCCCAGCCCTCAATGTGCACGTTATCTCATGGGGGAAG 
               
               
                 (SEQ ID NO: 130) 
                 GAAACACATGGACAAGTGGGGGGTGTCAGAGGTACTCAATGGT 
               
               
                   
                 GGTACATAGGGACAAGATTGGCATGCAGTGAGCAGGGGCAAAT 
               
               
                   
                 CCCCCCAAGTGTGGGTAGGTGAATGTGCTGGAAGCAGGTTGGAG 
               
               
                   
                 GGGAGGAGTCAGGAAGATATGCAAGA [A/G] GAAGTATCCCAAC 
               
               
                   
                 CAGTTCCAGCTCCCAGCTTGACCCATAGAAGGGGGAAGACATAA 
               
               
                   
                 TTAAACTGCCTGGGGGCATCCAGGAAAATACTGGTGAAGACTCA 
               
               
                   
                 GCAAGGTTTCTCCATCCTTCAGTCAGTGCACTGAAGAAGTGCAG 
               
               
                   
                 CTGAGGAAAGAGCAGTAAGTTAGTGGACAATGACCACACACAC 
               
               
                   
                 CAAGGTGTGCGG 
               
               
                   
               
               
                 BICFG630J24664 
                 CCTTCCCTCAGCACAGCCCCTGGCTCTTCACGGTCACTTGGAGGC 
               
               
                 (SEQ ID NO: 131) 
                 TGCCTCATGGCTCCCTTGGAGCTGTGCTTGCCTGGGCAATGGGCT 
               
               
                   
                 AGTTCCTTCAGGGTTCAGAGGGCTGGAATGAGACCCTACTTGCT 
               
               
                   
                 GTTGGCTTAGTAGACTCTACCCTGGAGCTGACAAGGGGAGGTGG 
               
               
                   
                 CTCCACGGGCAGCCCTGCTCTC [A/G] CTGCCCGACTACCTGTGGA 
               
               
                   
                 CACGTGTGGACACCGGCGTGCGAGTGGCCCTGGGGCCCCTGGAC 
               
               
                   
                 CTAGCATTCTTCCCAGCCTCCACTTCAGAACTGGGATCTCTTAAC 
               
               
                   
                 ACCTCTCCCCACGTCTGCCTCTGGCATCTGCTCTTCGGGCCCTCC 
               
               
                   
                 CCCGGGGGAGGGGGCGGGGGGGAGTGGGGGGAATGTTGCTCTT 
               
               
                   
                 GCTA 
               
               
                   
               
               
                 BICFG630J27518 
                 TGTGAATAATCTCTTATAAAAGCAGTAAAGATCATGCCATTATA 
               
               
                 (SEQ ID NO: 132) 
                 CCTGTTGAATTTGCTGCAGTTTTAGTTCTATTTTAAACAAGGTGT 
               
               
                   
                 CATGAAAAGCACAGACTTACCTGTACGGTAGACAAAGTTGCCTT 
               
               
                   
                 CGGTTTCTGATGATGAGGGAGACACCAATTCTTCCTCAAATTCA 
               
               
                   
                 TTGGAACTAAAAGATCCCGAATG [G/A] TTTCTTTGCCTTGTCTTT 
               
               
                   
                 CCCATCATGGCAGCATTTGTGGCCATGACATGTCTCAAGGAGTC 
               
               
                   
                 GTTAAGGTAACCGAATTCAAATAAAGCTGCTCTTGTATTNGGGG 
               
               
                   
                 GGGTGAATACGTAGTCCTCACTGGCCTGTGACTCTGGCCTCACT 
               
               
                   
                 CCAGCTTTTATGACTGAGCTTTCACTTTTANTCACAGGATGATGA 
               
               
                   
                 ACTGG 
               
               
                   
               
               
                 BICFG630J34588 
                 GGATAATTGCAAGTCATAAAAGAATTAAGACATTTTCTTCCTGA 
               
               
                 (SEQ ID NO: 133) 
                 AAAGACTAATTGAAACTCTAAGAAATGTGAGTTACATAGAACAT 
               
               
                   
                 GCTGGCCACCATTTCAGCCATTTTTGTCTTTATTGAAAGGGCTGA 
               
               
                   
                 TATTTTATTTCCAAGGAATTGCAAGTGTAGTTTTTAAAATACATG 
               
               
                   
                 GTTGAAAATATGATAGACGTTA [C/G] AATGCTGAATTAGAGAAT 
               
               
                   
                 GACTGATTTGAAAAGAGGTGCCATAAAGCTGTTACATTAACCCT 
               
               
                   
                 TCGTTGAACATCATATGTTTGATGGTCAAAGTCTCCACGAAGAT 
               
               
                   
                 AGACCGCCAATCTCATAAGGCACACTAGGGCGCTAGGTGAAGCT 
               
               
                   
                 CACAGATGATCTCATGAGCTGGAGCCTGCAGGAGGAAGCGTTG 
               
               
                   
                 GTGGGCA 
               
               
                   
               
               
                 BICFG630J36601 
                 CTTCCTGCCTATAATTTCCATAGACCAAAAGTCTTCTTTCCCCTT 
               
               
                 (SEQ ID NO: 134) 
                 AAACTAGAATAATTTCTTCTTTTCTCAATTCAGTTTTCCTATTAG 
               
               
                   
                 AACAGACTANAAGGGAGGTTTTTTTTAAGATTCTGGGCTCTCAA 
               
               
                   
                 CTTTTTTTTTTAAGGCAACAGAGACATCTTTTGGCCAATTANTGC 
               
               
                   
                 AACTGCATGGTGATAGTAATG [A/C] AAAGTTAATACACTATGAG 
               
               
                   
                 CTGCATTGGTGAGCCATTTTCTATGATCTGTTCAGTGATTCCTCA 
               
               
                   
                 GTCCNGTGACGTTTCAAAGCTGATACAGCATTGGCCCACTGACC 
               
               
                   
                 ACAATAGGAAGTTTTTCTGATAAAGAAAGGCAAGAGTCAGGAT 
               
               
                   
                 CTGGATCCACTACCTGAAATGCAGTTCGATCTGAATGGATCCTT 
               
               
                   
                 GGGTG 
               
               
                   
               
               
                 BICFG630J39325 
                 CACATCACGGACACATTTCCTATGATCTCTACTCCCNCTCTTTTG 
               
               
                 (SEQ ID NO: 135) 
                 TCCTGTAAAGTAGGAAGCAGTAAAAGGCTATAATCTGGGACAC 
               
               
                   
                 ATTCTTCTATGGATGGATTTGGGGAAAATAAAACTTTTTCACTTT 
               
               
                   
                 TTCTCAGGTATAGTGCTATTACACTATGTTATAATTAAACATAAA 
               
               
                   
                 TTGCAAATATCACGAACATAAC [C/T] GTGATACCTTATTGAATTC 
               
               
                   
                 AGGAATTAGCCTTCTCGTGAATCTTCATGGTTTGTGTAATGAAA 
               
               
                   
                 GCTGGGGCAGTAGGGAACATTGTTGCTTCAGTGTGGTCTCCTTCT 
               
               
                   
                 GGCTGTATGGCTGCTGTCCCATTTCACTTCAAGCATTATTTATCA 
               
               
                   
                 GGTAGTTTCAGCCTCAAGATCTTTATGAGACCCTTTTAAAATATG 
               
               
                   
                 TT 
               
               
                   
               
               
                 BICFG630J54631 
                 ACATGCACAAAACAGGAAAACTGGTTGAAACTCACTGGTGGCA 
               
               
                 (SEQ ID NO: 136) 
                 CCTGGGCAGTCACTTTATGGGCCTACTGAATGTTTCCATGGAAG 
               
               
                   
                 TAGCCAAGGGAGACACACACTGCAGAGCNTCGTAAGTTGGCTCC 
               
               
                   
                 TGACCACAGTTTGGCAAGGTGGAAGCCATATTATGGGACATCTA 
               
               
                   
                 GGAGGAANCCCCTTGGGAGCAAGGT [A/G] GTGAGGGTCTCAAAA 
               
               
                   
                 GACACAAAGTGTTCTAGGGCTTACTTATCTTTTTTAATGGTTTGT 
               
               
                   
                 Gtggatttgagaaaatagtcaaaatgaaggataatagagggatgaaactgtcctacagagcaagagaccc 
               
               
                   
                 caccagtggaacaaaactacacacaagatattagatattaggtataagatagaacaagagtatacttccc 
               
               
                   
               
               
                 BICFG630J64739 
                 GGTAATACCAATAAAACATCTATTGGTAACCTACTTCTTCCCTAT 
               
               
                 (SEQ ID NO: 137) 
                 TCAAATGGGCGCATGAACCAGATGCAACAGGGAGATGGAAACA 
               
               
                   
                 ATTTGCCTGACTAGTTGCTTTTCAGGAGAGTAGGGTGAAAGTTC 
               
               
                   
                 TAGTTATCCTGTGGGGTTCTGGGGCTTTAACTCTACTGCCTGTAC 
               
               
                   
                 ATTTAATGTGAATGAACCTATTC [A/G] GTTGTTAGAATTTAAAAT 
               
               
                   
                 ATGTAGAAGTTGTTTATAGTTTGCTATATTTCTTTCCTAACGTTG 
               
               
                   
                 CGGGTTTTTTAAGAGAATGATTAGTAGGTAGAACTTTAAAACGT 
               
               
                   
                 TCATCTGGATCTGAACCGAATCCTATTTTATAAATCCCTTGCTTT 
               
               
                   
                 GCTGAAATAGGTGCAGGAAAGGTACGCTACACTTGATTTTAAAT 
               
               
                   
                 TAAG 
               
               
                   
               
               
                 BICFG630J74970 
                 TTGTGCCCACACAGGACCTCCAGGGCTCCCCTTAGCCTGCTCTAT 
               
               
                 (SEQ ID NO: 138) 
                 ACATCAGTCAGATGGCCTCAGGTTTTTAGCTATTTAAAAAGTAA 
               
               
                   
                 TCATCTAAAAAATAAGATTTGTACGGTGTAGTTGTTACCATCATT 
               
               
                   
                 TTGGCGACATTTATAAAGCTGCATCACTGGTAATGTAGGGCTTT 
               
               
                   
                 CCACCTACATTTATCTTTAACC [T/G] CATGCAAATTGGAATCAAG 
               
               
                   
                 AACAGCTGATGCAGCCTAGTAAACCCATGGTAGAAGTTTCCAAA 
               
               
                   
                 AGAAAGGAAATAAGTACACGGTCATAAATGCACTCTTATTTTTA 
               
               
                   
                 TCAATAACATTTAAACATTAAATGCTAATTATGTAAAAACTCCC 
               
               
                   
                 ATCAATAAAACCCCATTTATAATTTGCAAGGATCACTAGAAGTT 
               
               
                   
                 GGATT 
               
               
                   
               
               
                 BICFG630J79584 
                 AGAACTCCTCTGTTCTTTCTTGTCCTCAAATGGTGGTGACTGTTT 
               
               
                 (SEQ ID NO: 139) 
                 TCATCAACCTATGCCTCCCCGCTACAGGCTTCTCAAGTTTGCAAA 
               
               
                   
                 TACTGCTGAGTTTATAAACGGTTACACACAAGCTGTACCTACCA 
               
               
                   
                 TGGTGACAATGAGCACGAGGAGCTTCAGTAAATACTGACAGGTT 
               
               
                   
                 TTGGTGGGAGGCCCACCCTTCT [G/A] TCCTATTTCACACTCAAGG 
               
               
                   
                 AACCTGCCCCATCAATCCTGGGGCTTCCTCCCTTCCTCAGGCACC 
               
               
                   
                 TGGACCCTGCATCTTCCCTCTAGCCAAACGAGACATTCCTGCCC 
               
               
                   
                 AAGGACAGCGAGGCAACTTGTTTCTGCACTTGCAGCACTTTGCA 
               
               
                   
                 GATCACAAGGCCTTCCGAGAGTGGGAATCAGTGAACACCCAGA 
               
               
                   
                 GATCC 
               
               
                   
               
               
                 BICFG630J89999 
                 GATCTCAAAACAGGTCCACCCTGGCTCATGCAATCTAGCCGAGT 
               
               
                 (SEQ ID NO: 140) 
                 TGGGGTGACACCAGCTCTGATCACTTGAGGGGCGCAGCCAGCAG 
               
               
                   
                 TATTGAGCCTCTCTATGTACTGGGCACTGTGTGTGCGTTTACACC 
               
               
                   
                 AGTTCTCACAATTCAGTGACACACATGCAGGAGGGCGGAGGGG 
               
               
                   
                 AATAGTAATAAAAGAAGTTTCCAG [A/G] AATAGTAAGACCAACT 
               
               
                   
                 TTTAACAGCGGGTAGATAGGGAATAAAAAAACGTTTTAAAATTG 
               
               
                   
                 TCAAACCATTTCCTTTCTATTTCTCAACGTAGGGCATTGCCGGGA 
               
               
                   
                 GGGGCACGGATCNAAGAACNAAGTCCAGGCCTGCCTCGTTGGT 
               
               
                   
                 GTCGGAGNACAGCCCTAGAAAACAACGTGACTCTGGGGATGTA 
               
               
                   
                 CGTCAGGGA 
               
               
                   
               
               
                 BICFG630J98358 
                 Acaccgggctccctgcgtggagcctgcttctccctctgcttgtgtctctgcctttctctctctgagtctctcat 
               
               
                 (SEQ ID NO: 141) 
                 gaatcaataaataaaatactttaaaaaGTAAAATAAAAAAGAAACAGTGCTCTTC 
               
               
                   
                 CTACATAGGGAGACTAAATGATAGCTGCTGTTTGGGGTGAGTCT 
               
               
                   
                 CCAGACCAGAACCAGACCAGGGTTG [C/T] CCAATCTAATATAGA 
               
               
                   
                 TTGAGGAGTGGGCCAGATTTGCAGAGGCAGAGGGGAGGAGGGA 
               
               
                   
                 CAGACAGGGACTCATGGGACctgtgtgaagcctgttacgcacattatgtcattcaagcgt 
               
               
                   
                 aaacagacctgtgggtaccggtgctagaattacctccatttcacagatgtggaaagtgagactcagaCCC 
               
               
                   
                 CAAGAGCTCGTT 
               
               
                   
               
               
                 BICFG630J101630 
                 TCTTCCCAGTAGGCCAATGTCAGTGGCACCATCTCAACCATAAG 
               
               
                 (SEQ ID NO: 142) 
                 GGAAGTTAAAGGATCCCTGTCCNCTGCTCCATTTCACTCCCAGG 
               
               
                   
                 AGAGAAGAACTTTGATGAAAATCAAGAGGAGATACAGTGGTGC 
               
               
                   
                 TCCTGTCTTAGCAGGCAGAGCTAAGCTGTGAGAAACCTCTGCTG 
               
               
                   
                 GAGATGACCACNCTCATCTGGATTG [T/C] TTATTTGGCCAATCTT 
               
               
                   
                 TTATGATCTTTGCTCTCAGGTAGTATCTGGGCTGCCTTCTGCTGA 
               
               
                   
                 GGAGGGCCTCCTTCCCTGAGATTCCAAGCTGGATTGTCAGAGGG 
               
               
                   
                 ACCAGTGGAGAGACNGAGTTGAGGGGACCGCACAGACTGGGTC 
               
               
                   
                 TGTGGCCTCAGCGGAGACTAGCTTGTTTCCCTAGCTTAGTACGCC 
               
               
                   
                 TGTGCAC 
               
               
                   
               
               
                 BICFG630J111559 
                 ACTGCACTGCTTTCTCCTTACAAAAATTTCACGATTTTTGTGCTC 
               
               
                 (SEQ ID NO: 143) 
                 TCCTTGCTTACATCTTCTAAGTCTTCTGGGTCCAGTCTTTATTTCA 
               
               
                   
                 ATCATGGGCCAAAGCAGTTCATAGTGAGTGACTAGGTTCTATGT 
               
               
                   
                 CTCCTACGAAAGGCTGCCACTCCCAGGGTGAGTTAAGATGACTC 
               
               
                   
                 TGNTAAGGCCGGTTTTGAATG [T/C] TCCGTGGTGAACTCAAGACT 
               
               
                   
                 GCTGCTCCAAAGCAAGAACTTGGGCTCATAAAACAATAGTGTTA 
               
               
                   
                 TTACGATCGACGCAAAAAGGTGTCTTATTAGTTAAGTTGCTGCC 
               
               
                   
                 CTGCTGCCAAGAGCAGTGATCTAGCTTCTAATTTTCCTTTTTCAA 
               
               
                   
                 GATGATCAGGATGAGACTCATCAGATGGTTTTGCTCAGATTAAA 
               
               
                   
                 GGA 
               
               
                   
               
               
                 BICFG630J113042 
                 GCCACCTCTCTGAATCTTTTCTGGTGTTCTCACTATTTCCTTTAAC 
               
               
                 (SEQ ID NO: 144) 
                 TTTTTGTCACATTCACACATTGCTTAAAAAGTGTGGAAAAGGTA 
               
               
                   
                 CCTCTTTTGGAGAAAGAGCTTAAGAGTGAACACTCAGCTACTCT 
               
               
                   
                 GTCTCTTGCTTTACTCTGGGTTGGGAAGCATACCTGGCCCAACTT 
               
               
                   
                 GGTGCCAGTGCCCACCAAAAC [A/G] AGGACATCCCTGGCCCAGT 
               
               
                   
                 TCATGTCAGGCNTCAAGAGCAGGAAGACGTGAGGGAAAGAAAG 
               
               
                   
                 GGACATGGAGGTTAGAGCTATTAGAGCAAATCACCCTGTGCTTC 
               
               
                   
                 CTAGGGGTCTGTGCTGATCCTTTTCCACCTCCTGAGGGTCAGGGT 
               
               
                   
                 ACTTTCACTTGGGATGTGCTCTACGACAGGCAGCATAcaaacacaccca 
               
               
                   
               
               
                 BICFG630J120171 
                 CTCTTGGTGTGCATGAGGGGGGAAGAGATATTTGTGTACATGAA 
               
               
                 (SEQ ID NO: 145) 
                 GGCCACACCTGGGCCAGNTTCCATGAACTCCTGTGCTGACTCCT 
               
               
                   
                 GAGCTAAGATCTCCTGCCTCCCTGTACCTCCCTGGGCAGCTCTCT 
               
               
                   
                 TGAAAGCAGCAATTCCCTGATGGCCCCAGTTTCTTGGTCGAGGG 
               
               
                   
                 CCTAGTGGCCGCTCTTCCCTGCT [G/T] GGNCCTGATGGGTCAGAC 
               
               
                   
                 ATCGGCTCCCCCTTGCCAGAATAATGGTTCGGCCGCAGAAACCC 
               
               
                   
                 ACACAATCTCCCTGCGAGACAGGTCTTTGTTGTTCTGATGTCCGT 
               
               
                   
                 GGTCCCAGACATTCAGCAGCTTGCACACTCAGGCTCAGTGTACA 
               
               
                   
                 CACTTANACTGTCTTGTCTGGAATCGCTAACTGAGCCTGACNCCT 
               
               
                   
                 TAGG 
               
               
                   
               
               
                 BICFG630J132438 
                 TAGTAAGTTAATGAGCCCCTGACATATTTCCAGGAAATGTTCAT 
               
               
                 (SEQ ID NO: 146) 
                 CTTCACCAAAAATGAGCCATTTCTCTTCACTCAAGAGTTACTCTT 
               
               
                   
                 CATTCTACTGTTTTCATGGTTTTCAGATACTTTATACATTACTTGA 
               
               
                   
                 GNCTCGCCACGATCCTCAGAAATGCACAGGAACCCATCAGTTGG 
               
               
                   
                 CGTCCTCCGCAGACGAAGCCA [C/G] GGTTCAGAGGCGTCAAACA 
               
               
                   
                 GCACGTCTGGAGCCTCGTGGTTAATCAGCAGTGGATTTGGGTTA 
               
               
                   
                 AACCCAGGCCCTTGTTCTCCAGACCTGTTCACGCTCAGGCTGAC 
               
               
                   
                 ACGCTCAGGCTCCGGGACCACGGTCACTGTCACAATGCTTCCCT 
               
               
                   
                 CCCTTGCTCATCATTTTCAACTATTTAAAAGGAAACTCAAGGGG 
               
               
                   
                 NTGGA 
               
               
                   
               
               
                 BICFG630J137139 
                 GGCTTCCTCTGTCCTACAGCTCATCCCAGAGCAATGAATCTGCCT 
               
               
                 (SEQ ID NO: 147) 
                 GAATCTGTTGGTGACAAGCTAAGGGCATGGGCTTCTCTGGGCAA 
               
               
                   
                 TTTGTGGTAGGATAAGACTGGTGCTGGAGACAGGAGAAATTGCG 
               
               
                   
                 GGAACCCTTTCTGCCTTTGAGCCACTTCATTCCCAGTTCCNGAGG 
               
               
                   
                 AGAAGGCTAGGGGTGGGGGTAG [C/T] TTAGCCCAGGGCCTCCTC 
               
               
                   
                 CTGGGGGTGGAGTTGACCTTGAGAGGAGCAGTGGCATCTACCAT 
               
               
                   
                 CCCTTCCTCTTGGGGCTCTTCAGTGCCAATCTGAACAGTCTGGAA 
               
               
                   
                 ATGGAATCTCTGGGACCCCTCCCCATCACTATTATTATAATAAAC 
               
               
                   
                 TCCAGGATTGTATCTGCGGAGCCTCAGGCTTCAGGAATGCAGCC 
               
               
                   
                 TGTA 
               
               
                   
               
               
                 BICFG630J145174 
                 GTGTAGAAATGGAATGGAAAATTCAGTTGAAACACACACACAA 
               
               
                 (SEQ ID NO: 148) 
                 TGTCAAAATTCAGTTGGTTTTACCATAGGAGATTAAATTAGCCA 
               
               
                   
                 AACAAGGGTTCTCAGTCTCTATTTTAGGTCAATTTTGAGGTTGAT 
               
               
                   
                 TATGGTTTGTGAATATTTAGTGTACTGTCAGTTTCATAAAAATAA 
               
               
                   
                 AAGGTAAAACTTTTCTCCCTGAT [T/A] GTCTGAAGTGATGAGAAT 
               
               
                   
                 TTATATATTAGCAGGCAACCCCAGGAAGCAGTGTCTCTAGTGGT 
               
               
                   
                 ATATCAAAGGCCAACATTAAAGTATTAACTTCCCTAAACTTAGG 
               
               
                   
                 TTTATTAGGTTTTATATCTGGTATCAACAGACTCTTATGCTTCTA 
               
               
                   
                 GTCAGAAGATTTTTAAGAGGATAGACATTCTAAACAATGCCAGG 
               
               
                   
                 ATCAA 
               
               
                   
               
               
                 BICFG630J156161 
                 GTGTGAGCGCGGATGCATAAGGATGGCGCAGAGCTCTGGACTC 
               
               
                 (SEQ ID NO: 149) 
                 AAGCAGATGAAACAGGGTGGGAGTGAACACTGGACGCTGGAAG 
               
               
                   
                 GACAGGCTCAGGCAGGAGCAGTGGGGAAGCACGCCCTCCCCGG 
               
               
                   
                 TGCTGCTATCTTTCCGTGTCAGGACACAGCCGCACAGTGGCTTTT 
               
               
                   
                 GCTCATGCACGTGCAGCTATGTGTGT [T/C] GACAATTCCAGCTAG 
               
               
                   
                 AAGGGTGCAGAAACTAAGCAGAACTTGACTGAGTAGGACAGCG 
               
               
                   
                 GGCAGCAGGAGGGCCGCCCCCGTCACCGGGAGGGTCACACGTG 
               
               
                   
                 CAGCTCCAGCCAGAGGAAGCTGGGGCACGTCGGTCCGGACCTCC 
               
               
                   
                 GCAGTATGTCCGCGGTTTGTCCACGGCATCAGGGGACACCGAGG 
               
               
                   
                 CAGGGTAGCCA 
               
               
                   
               
               
                 BICFG630J156875 
                 Ctagatcctcttttacaggtgtcatccactctcttcttgatcacatccaatgagattatgatacttactcagctctc 
               
               
                 (SEQ ID NO: 150) 
                 gcaNgccaagagagctgagtcagtatcatttctttgttctattgttttagtNgctagaaagttcttAACAG 
               
               
                   
                 TAAGGGATATCTATAATTTTCACACATTTTCCAAGTTTTCACCCA 
               
               
                   
                 TCNAAAG [G/A] GGACCAAAGGTTTGAAGAAAGTTTCCAAGTGTG 
               
               
                   
                 TCTTAATTGTTTTACTCCNGGTGAAATATCCAAGATCTTTCAGCN 
               
               
                   
                 ATAGATAATAAAGTGTACAATTAGAANATTATTATCTACTTCAA 
               
               
                   
                 TCAGGGCACTTTATTTCTGCAAATGGGAACAACATTNAGCACTA 
               
               
                   
                 TTACCTTCTTTAGCAGCTCTGACCACTTGATTGT 
               
               
                   
               
               
                 BICFG630J160536 
                 AAATCAAGTTCAAGGAAATATTATTTCCTGCACGCCAGGTAGAT 
               
               
                 (SEQ ID NO: 151) 
                 GTCAGGCACTCTGCTGGTGCTCTGTGTGGGCGATCCCCTTTCCTC 
               
               
                   
                 CTCCCCATATTCTGAATGAGAGGGACTGTCCCATCCTTTCCTAGG 
               
               
                   
                 AGGAGGAGCCTTAGGTTCTGTGGGGTGAGGGACCAAGTCTACTT 
               
               
                   
                 TCACACCCATGCTCCTTCCTCT [T/C] GTTGACACCTTCTTGAAAAT 
               
               
                   
                 ACAACAATCCACATTTCGAGTGCTATCGTACCAGGTCAGACAGC 
               
               
                   
                 CCACACACCCTTAAAAATATTTCCTTCTCCCTCCAGTCTTTTCCT 
               
               
                   
                 CAGAGTAAAAGCTTTCGGGAAAGGCCCAGATGTGCTATAAGGG 
               
               
                   
                 AAACTCAGCAATTATGCAAGTGAGAagcacagcccaatggttagagcg 
               
               
                   
               
               
                 BICFG630J164406 
                 GCCGTGGGATCACGTGATGCAGTTCCTGGGAAGTGGTTGGTGAG 
               
               
                 (SEQ ID NO: 152) 
                 TCAGTTTCGTGCTCCTTGCCCAGCATTGGTAGTTTGGNTGCATGC 
               
               
                   
                 AGACTGGGAGTATCCCTGAGGTGAAGGGAGCTTGATGGTTATAT 
               
               
                   
                 GTTCATAGTGATGAATTGCAATCATGCTGGAGCCCGAGTCTGTT 
               
               
                   
                 TCCCCAAAGTGCCTCATTCCAGA [C/G] GCCCGCAGGCATCTGTCT 
               
               
                   
                 CTTGGAGCACCCTACCNCGTTTGCATAGGGCAGTGCATTCAAAG 
               
               
                   
                 ANACTTGGGTGGAGGACATCTGGCATCATGTCTTCCATATGTCA 
               
               
                   
                 AGGCCGAATCNAGCAGGTGGATAGAAACAGGACTAAANCAGCA 
               
               
                   
                 TCCNTGANCATACTTCCTNAGTGACCAGCAGTCCTGCTCTGATTT 
               
               
                   
                 ACCCAG 
               
               
                   
               
               
                 BICFG630J168764 
                 TGGGCATAACTGCCCACAGTGGGCAGCACATGGCCCCAGTCAAG 
               
               
                 (SEQ ID NO: 153) 
                 TAAAAGACTCCTTACCACCTTCCCTGAATCTTTCTCCCCAGTCCC 
               
               
                   
                 TGTATATTGGAGTAGAAGAGACACAGGAAGAGGAGGATGTATC 
               
               
                   
                 CCCAGAATGAAGGAGCAGACCACAGCCCAACTCCCCACTAAGG 
               
               
                   
                 AACCTAGAGCCATTGGAAAGAGCTA [T/C] ACTGGAGGGAANGGA 
               
               
                   
                 GGAGAAAGGCAGGGCCAAATTTACTNGAAGTTTCACTGCATCAG 
               
               
                   
                 ACTGAACCAGGCTTTGTGAACCTAAGTGGACATANGGGTCTGAG 
               
               
                   
                 ATTTGCCCACGATATGATCAAGGGGTGGGGAAAGGGGCTTTAAT 
               
               
                   
                 GGCCAGTAAGTGAGGGAAAGTATTATATGCTTATATTTTTCCTG 
               
               
                   
                 CTAAGTCAG 
               
               
                   
               
               
                 BICFG630J178333 
                 AAGAGCAGCTCTGCCTAGTGGTAAGGCAAGGGAGGTTGACATTT 
               
               
                 (SEQ ID NO: 154) 
                 GGATGGGTATCTGCCTGGTCCCTGCTGGCAGTAGCCCAAAGAGC 
               
               
                   
                 ACTACTGTTCATGGGGATGGCTTCCATGACCTGAGCCAGGGGTT 
               
               
                   
                 GGATGGCAGCCTTAGCTCCCAGGGTCTGCTGGTGACCTCTGCGT 
               
               
                   
                 CAAGGGGGTGATAACTGGGTCCCA [T/C] AGTGCCTTGTCCACCTC 
               
               
                   
                 TTTTACACATTAGGTTGGCCTTCTTGTAGAAGCAGAGTGACTTCT 
               
               
                   
                 CGGGCCATGGAATGTGGCTCTTTTCCCAGGAGGCCAGACTAGGT 
               
               
                   
                 CTGGGACAAAAGCTTTGGGCCAGGGGTAGAGCTAGCTTTGGAGT 
               
               
                   
                 GAGCACAAATATGCACGTGTGTGTGCACGATGTGTGTGTGTACC 
               
               
                   
                 TGTGTG 
               
               
                   
               
               
                 BICFG630J182918 
                 TTTAAGAGGCTGGTCTTCTGAGGAAGAAATCACAAAAATTATAA 
               
               
                 (SEQ ID NO: 155) 
                 ATCATAAGATGGCATTACTGACATGAGAGTGAATCACACTGGTA 
               
               
                   
                 CATNCACAAGTGACGGGCTGGTGCAAGAGACTTTAAAATAAAT 
               
               
                   
                 GTTTGAAATAATCAAAGACANAAATGAAGGACTAAGTGCATCCT 
               
               
                   
                 GCAAGAAGAGGGCAGTTTGAGAATG [T/G] CTGCTTTGCAAACAA 
               
               
                   
                 TGACAACAACAAAACAGATTTTAGAATCTAAATAAATAGTATTT 
               
               
                   
                 TAAAAAATGTACAATAGAAAAAAATGGATCCTGATGATAATTTT 
               
               
                   
                 GAGGTTTCTTTCTGGCAATAACTGATATAAGAAGCTCCAGAATT 
               
               
                   
                 CTGAGTACATAGTGTGTGTGTAGGTGCACACACATGCACGTGCA 
               
               
                   
                 CATGTCTGT 
               
               
                   
               
               
                 BICFG630J190167 
                 ggactcgatcccaaacttcaggatcatgacccaagccgaaggcagatgcttaactgactgagccacccag 
               
               
                 (SEQ ID NO: 156) 
                 gtgNcccTAGGCATGACTGTAAGACTTTTGATTATCCTCATGCTGTA 
               
               
                   
                 GAGGTGGGTAGTATATATATCAGTGCCATGTGATTCATTTGAGC 
               
               
                   
                 AACTTCCTACTGAGAATTTATTTACAAGGATGTGTATAT [C/G] CT 
               
               
                   
                 CCTGGTTGAGAGCTCTGAAAATGATGTAAGGCAACATGAAGATC 
               
               
                   
                 CTAGAGAATATTTTATTTACACCCCCTAGAAACTTTTAATGTCAG 
               
               
                   
                 CAGATGCTAGCTAAGGTGTACTTAGCGTCTGCTTGTGTACCCGA 
               
               
                   
                 TGTGCTAGGTAActctgtgcctctgtttcccattgacaaggtaggaatagtaagagtgtctacat 
               
               
                   
               
               
                 BICFG630J209785 
                 GTTTGGGATCTGGCTCTTGGATGTTACCAAGTATCTGGGGACAA 
               
               
                 (SEQ ID NO: 157) 
                 AACTCAGAATAACTAACTTTCTTGCTTCTCCTCGAAATGGGAAC 
               
               
                   
                 ATAGCTTCTTTGGCTTATTCAAAGGCAAAATATGGGTCGTGNGT 
               
               
                   
                 AAACAGGGCCTAGTGGAAAAACAGTAGAGATGGAGCCTTTCTA 
               
               
                   
                 AAATGGATTCCCTTCAGTTTCTCTA [T/C] GTCATGAAGGCTCACT 
               
               
                   
                 GCCACCGCCTCTCAAGCAGAGCCACAGTTTCACTGGAGTGGCCG 
               
               
                   
                 CCTGGCGTGGCCATNGGGCCAGACACCAAATGCAAGAAGCACA 
               
               
                   
                 AATGCGTGACAGAAAGAGCTTCCCCTTTCTGCTCCCTTCTGTGGA 
               
               
                   
                 TCGCAGATGCTGCATTTGAGTTTTGGGACAGCTGTTTTCCCCCGG 
               
               
                   
                 GGGGCTC 
               
               
                   
               
               
                 BICFG630J215562 
                 ACCGGCTTGTAACTGGCAGANCCCANTAGGCATTTTCTNAAGGA 
               
               
                 (SEQ ID NO: 158) 
                 TACTGGCACTGGGACATCCACCANTCTAANAGGGAAGGTATTAT 
               
               
                   
                 CTAGTGCTGCTTGATNNTATTTCNGGATATGCCTGCTTCCTTTTC 
               
               
                   
                 TGCTTACAGAGTTCCTATGTCCCTTCTTCTTGTTGTTGTACCGTGT 
               
               
                   
                 GCTATGACTGCCATCACTTCA [C/A] TCTGCTGGTTGTTTGGAGGT 
               
               
                   
                 TTGTTTTNTCTCGACAGAACTCAGAGATACAGGGTAGGAGATGG 
               
               
                   
                 GATCGGTCGTGTGCACCCTCAGCCTGACAGGCACATANGCACNG 
               
               
                   
                 GCATCGAGGAGGACTATGGGTTGACTTCTCATGAGAGTAACAGA 
               
               
                   
                 ATCCTGAGGAGAAGAGTCATACGATCCACTCTTTCATCAGATTC 
               
               
                   
                 TCTT 
               
               
                   
               
               
                 BICFG630J227421 
                 TTTATTAAATAAGATATCCCTTCAACATTGGTCTGTAATGCTTCC 
               
               
                 (SEQ ID NO: 159) 
                 ATTAGCATGTAGTTAATTCAGAGACACATCNATGCATATTCCAT 
               
               
                   
                 TACATTTTAATGCACATCAATTATTTTGTCAGTACCACACTGTCA 
               
               
                   
                 ATTGTCAAAGCTCTAATGAAGTGACATATGATGTTATTCCATTAC 
               
               
                   
                 AGTATCTCAAGATGTGAACTA [C/T] CAGTTCTGGGTCATCTTACC 
               
               
                   
                 TTTCCTTGCAACCATCCCCCNCGCCCNCCCCCCNCACACACACA 
               
               
                   
                 CTTTATTTGTGNAGGATAATTCCGAATTAAAAATAGCAAAACTC 
               
               
                   
                 TACACTGTCCTGCNAGATGTACAATTAAAANGATGAAGAAAACC 
               
               
                   
                 AAAGACCCAGTTTTGTCTTCCCCAGCTACCAGAGTGAGCCCAAA 
               
               
                   
                 AATA 
               
               
                   
               
               
                 BICFG630J232150 
                 Aaatggcatttccatgtcacattcctatgtccccaaatcaggatttgaagccggttgttctgacttacggccca 
               
               
                 (SEQ ID NO: 160) 
                 aaactcgtttcaccacaacaGACGTACAAGAAGAAAGACTAACAAATCCAT 
               
               
                   
                 TTTATGAAGTAAAGGATCTAACTACAATTTTTGTTCCAAAGCTTA 
               
               
                   
                 TTGACACCAGGACCCAGTGGGCTGGGACAC [T/G] GATATATTTT 
               
               
                   
                 ATCTTCCTGTACNTCACAGCTGTCCAAATCTTGATCTCTTCAATA 
               
               
                   
                 GTGACCCATTACACAGtctcatccagtcttctggatttaaataccatgtatattactaataattata 
               
               
                   
                 cattttatctctaacgtgacNctttNcattagataactaacatttcaatattattcatccaaaatcgaggtactga 
               
               
                   
                 ta 
               
               
                   
               
               
                 BICFG630J235932 
                 TGGTCACAATTGTTGACACTTCCAGCCCCAGTTCCTCCTAAAAG 
               
               
                 (SEQ ID NO: 161) 
                 GGATAAAAGAAAGGAAAGGATATCTAGTGGCAGGAAAGTATGA 
               
               
                   
                 AAAGGGCAAATCCTCTGACTTAGCAGAGGGACTGACACNGAGA 
               
               
                   
                 AACTTAGGTCAGGGTAGAGGTAGAAAAGGAGCACAGAGGAAGT 
               
               
                   
                 AGCAGTGTCTCTGAAGGAAGCATTGCC [A/G] TCACCAAGCCAAA 
               
               
                   
                 TTTATCCAAGGACTCACACATTTCTGTGACAGGATCCCTCAAAT 
               
               
                   
                 AAGAAGGCAAGTTTCCTGTAGAGAGACACAAATGAGAAAGGCA 
               
               
                   
                 GGGACCTTTTTTAGCAGAGCTGAGATTTTCTGGAAAACCTGGGG 
               
               
                   
                 AAGCACACACTTCTCAACAATTCAGTTAAATTCTTTACACTATCT 
               
               
                   
                 TTAGTTCAGAA 
               
               
                   
               
               
                 BICFG630J255886 
                 CTCCCACGCCGCCTCTCTTTCTATCCCAACACCTCCCATACTACC 
               
               
                 (SEQ ID NO: 162) 
                 TTGAGAAGCAGGTTCCTGCTCTGGGGATTGTCCTGGGAGCACAG 
               
               
                   
                 TTTTTCAAATGCTTGAATCTTCTTCCTGAGGAGAGAGAAAGAAG 
               
               
                   
                 GCAGGACGGATGTTGGAACACTAGGNTCGGGTTGAGGGCAGAC 
               
               
                   
                 CCTGTGATCTGAGACCTCGAAGGA [T/G] TTCAACTGCTGGATCAG 
               
               
                   
                 GGTTTCCTTGGGTTCCTCTGAGCACTCAGGTCGTTGAAGGACAC 
               
               
                   
                 GGGAGGGAGCTCTTTGGAGGGTCATCCGGTGCATCCGNTGCCCT 
               
               
                   
                 ATTAATTGAACNGCTCTGCTTTGGTCAGTTTTGGTTTCAATTGCG 
               
               
                   
                 AAGAGACTCTAGTTGCTGTCATGTTCGCATCCAAAACCTTGTAC 
               
               
                   
                 CTCAGC 
               
               
                   
               
               
                 BICFG630J265884 
                 CAACACAAATTATTTATACCATAGTGAGTTGAATATAAGAATAT 
               
               
                 (SEQ ID NO: 163) 
                 GGAGAGAAAAACAACTCAAAATGTATGTTTGACAATAGTGTATT 
               
               
                   
                 TTAATGAATTTAGTGATATACAAAGTAAGATAATTGCTTGTTACT 
               
               
                   
                 ACAAAGAGTTATTTTGATATAAAGAACCAACTACACAAAATTAT 
               
               
                   
                 AGTTTTATGTGTATTTTAGTCAA [A/G] AAATCATCTTGACAAGTT 
               
               
                   
                 TAATCTTATCAAGGGTTAAAATAGATATATTACAAATTGATATA 
               
               
                   
                 AAGACCTATATTTCATATAGANGTAATATACAgggatgcctgggtagctca 
               
               
                   
                 gcgattgagcatctgactttggctcagagcatgatcccaggtctggggattgagtcccacatcagggttcct 
               
               
                   
                 gcaagaagtctgctt 
               
               
                   
               
               
                 BICFG630J275606 
                 ATTGTATTTGCATAGCCCCTCTGACGACTTGCATTAGCTCGATTC 
               
               
                 (SEQ ID NO: 164) 
                 CATACAAACCCGTGTGCCCCAGTTTCATAAAGCCTTCTCTCCTTG 
               
               
                   
                 GCCAAATGAAATCAGCCTCTCCAAGTGACCCTCAACTTTAACAC 
               
               
                   
                 TTCAAAGTAAAGCACAGAGTTACTTTGATTATTACCACAGTACT 
               
               
                   
                 TGACCACAATCCAGAGAAAGTC [C/A] ATGAAAACCAGGACCAGA 
               
               
                   
                 TAGAGTTAATGCTTTCATAGAAACAAAATGCCGCCTGTGGATGC 
               
               
                   
                 TGAGTGCCAGCACATCATTAAGGGAAGGATAGGAATAAGGCCT 
               
               
                   
                 TCTTAAGAGCTGACATTAAAAATTGAAATCCATTCTGTAAAAGA 
               
               
                   
                 CAGGCCTTGTGTATTTTTTAAAGCCCAGAGCTATAGCAGCTGAA 
               
               
                   
                 GGGTAAT 
               
               
                   
               
               
                 BICFG630J278829 
                 attagcaagaaaattgcacggggaggtggcatgggaaggaaagaccagagccacagcctccctgcaag 
               
               
                 (SEQ ID NO: 165) 
                 ggctcttgcctCCGCTCTCATCTCCCNGGGGCAGAGATCTCGGTCCCCT 
               
               
                   
                 TCCCGGGCAGANTCTCCCTGGGGCAACCTTGCCAGAGAGAAAAT 
               
               
                   
                 GCTTCCTCCGGGTGGTGCTCAGACATGCCNTCTAGAACG [T/G] CC 
               
               
                   
                 CACCGGTGTGTCTCAGCAGGTACGCGGATGGGATGGCAGTGATG 
               
               
                   
                 GGGAGCCTNGGAGATTCCCTGCAGACNATCCAGACAGGAGCTG 
               
               
                   
                 AGGCTGTGCAAGGGACACTGAGCCTGCCCTCTGACCGCCACTGA 
               
               
                   
                 CTCCTGGTTCCCATTGCATCCTCTGGGTGCTGCCTCCTGTGCACC 
               
               
                   
                 CTGTCCTATTTCCCTGAAGTAA 
               
               
                   
               
               
                 BICFG630J282369 
                 GAGGCCTTGGAGATACTCGTCTCAGAGGTATTTGGAGAATAACC 
               
               
                 (SEQ ID NO: 166) 
                 TTGCAAGAACTCACTGGCTGGTGACAGTAATTCAGTTAGTTCTA 
               
               
                   
                 CATCTTTCTAGTGGAATCTAGAGTAAAATATACAGAGCCAGGTG 
               
               
                   
                 ATAAATTTTGAAAAAGGCCCTGATATGGCAGTGCCATGCATTTT 
               
               
                   
                 AAGTGTACATCAAGCTTTCTGGAG [T/G] CAGCTTGAGATGGATGT 
               
               
                   
                 TCAGTGTATTGGTGTCACATTTTTAGACATGTTCGCAAGGTCTCA 
               
               
                   
                 TTTTTTTTCTGTCTCTTCATTCTTGCATTTCAGCAGCTAGAAATGG 
               
               
                   
                 CTTTGCTGCTTCAAAACTCTGTGATATCTCTTTATGATAAATTTA 
               
               
                   
                 GATTTTAAATGCCATTGTCACTTGCTGAATGCATTTGTAAGAAAT 
               
               
                   
                 GT 
               
               
                   
               
               
                 BICFG630J304928 
                 TGGCTACCTTGGCGGAAAGCTCTGGTCCTNGCACAGGTGGCTTC 
               
               
                 (SEQ ID NO: 167) 
                 ACCCAGGCCCTGCCACTGCCTGCGGCNCCCTAGTGAGGCAGGGT 
               
               
                   
                 CCCATCCCTGCAGGTGCGGCCCNCGATGCCAGTGGATGCTCCTT 
               
               
                   
                 CTAGAACAGCTCACCCAACACGTGCCTTTGCCTTTTCCATGTTTT 
               
               
                   
                 TTCAATGTATCTCTGCCTCTTCC [G/C] TGCAAGATTTCTCTTGTTC 
               
               
                   
                 TAGAGATGNGATTGTACACCGAGCGGAAAGGGGTGGATCTGGC 
               
               
                   
                 GGGACCNGAGGGCACCCCACCCCCGTGTGCCTCACCCTGCCTTC 
               
               
                   
                 TGNACACCCCTCTGTGAGCAGGACCAGAGCTGCGGGCGCTGGG 
               
               
                   
                 GTTCCCAAGTCTGTGCGTCCATCCCGAGACCTATTCTGCAAAAG 
               
               
                   
                 GGGGATT 
               
               
                   
               
               
                 BICFG630J319569 
                 CCACGACCCGAGCCACAGCCCAGAGTCAGAGGCTAAGTGACTG 
               
               
                 (SEQ ID NO: 168) 
                 AGCCACAGGCATCAAGGCCCGGGTTCCTGCCGTGGGCACTCCTG 
               
               
                   
                 CCCCCCACGGCCCCCGAGAGCTCGGGCTGCGGGCTGCGCTCCCA 
               
               
                   
                 CCCCAGGCCTCCCGGCGGCTCCATGCACGTCCCCTCTGTCCCAA 
               
               
                   
                 CTCAGGGTGCAAGGGCCTCGGCCGG [T/G] AAGGCGCCTCCCATC 
               
               
                   
                 TGCCCGACGAAGCCCAGCCGGACGGAGCTGCTGGAGCAGGAAT 
               
               
                   
                 TCCAACAACTGCTGCCCGCCTTGCTGCGCAGGGATGTCATCTCC 
               
               
                   
                 GTTTTCATCTTTTTAGACAACTGTCATGGATTTGCCACCACCGAC 
               
               
                   
                 GAGGTGCTGGATCTGCTGTTTACGAGAGTGAGCACCTGNGCCTC 
               
               
                   
                 CGCAGCCCA 
               
               
                   
               
               
                 BICFG630J331636 
                 ATGTAAGAATAATCAGAAGGAAGTGAAATATATAAAAAACAAC 
               
               
                 (SEQ ID NO: 169) 
                 TTACTGGTGAAAAATCTCCAGTACAGGTTTTTTTATTCATTAAAT 
               
               
                   
                 CCTCTCTTGGTAAGGGTAAACTTACCTGTGGTAAGGCAAGGAGA 
               
               
                   
                 TAAGCAAGAGCCAATGTCATGTCATTTGGTAAAGCATCACTTGC 
               
               
                   
                 CAGCTGCAAGAGAACTGAAGGCAG [G/A] GGGAAAAAAATGCAT 
               
               
                   
                 TATTATTCTTGAGGATCTATATGACATTTGTAGTTACTGAGTGCC 
               
               
                   
                 ATGTTTTTAATCTTGCATGATTACCACAGATAATTAGCTTCAGAG 
               
               
                   
                 GACTCTTTAAGACCTTTTACAAATGCCTCTTAGTACCATCCAAAT 
               
               
                   
                 ACACATCATAGGAAAAATTGTTATTAAATAGTAACCCTGTCTTA 
               
               
                   
                 ATTCAG 
               
               
                   
               
               
                 BICFG630J346559 
                 GAGAGAAGTCTTCTAAGCGCATATCTGGCCTTTCATAGGGATGT 
               
               
                 (SEQ ID NO: 170) 
                 ATAAAAGGGAAAAAAACATTGATAGCATACAAAANGACATTTA 
               
               
                   
                 ATGATTTGCTTCTCTGAGATCTTAACTGTATCNGGCTTCTTTTTTA 
               
               
                   
                 CATTCTATATCCCTGACCTCTTCACCCAAATGCCTAGAGTTCTTC 
               
               
                   
                 CAGCTTCTAAATAAACAGTATG [C/T] ACTTGTTGCCATATTAAAC 
               
               
                   
                 CTTGATTCAGATCCTGCTTAATTTCCATAATCTTTTTAGACCCCT 
               
               
                   
                 ACATCTCACTTAGATAATGACCCTTTAGCTTGAATTAAACTATGT 
               
               
                   
                 ACCATTGATCATTTACTTGTTTTCTCTTGGAGTTTAATTTAATCAT 
               
               
                   
                 TCTAGTTTAGTTCAAAAGGACAAATAGCTTTCCACACAATGTTTG 
               
               
                   
               
               
                 BICFG630J356853 
                 CATAACTCCACCCCAATCCTCACACAAATGATTCCTCCTAAAGT 
               
               
                 (SEQ ID NO: 171) 
                 CACCAATGACACCCTTGCCACAGAATCAGAGGATCCTTTTCAGC 
               
               
                   
                 CTTCCTCTGTCATAAAGTCTCAAGAGCAAATCATACCTTCACTGT 
               
               
                   
                 TNTCCTTACATAAAAGATTATCTTACCCCAGAACCTCCTGATTTT 
               
               
                   
                 CTCCTTCTTCCATCCTGATCTC [T/C] ACTTCTCAATTTCCTTATGG 
               
               
                   
                 GCTCACTCTCCTCACCACAGCTTTTAATTCCTGGGAGCTTCAGTC 
               
               
                   
                 CTTTATGTCCTCCCTTTGCACACTCCCGATAAGATATTTAATATA 
               
               
                   
                 CTACAGCACAATCCTAAGGGTCTAAGAATAATTTTTAACACATT 
               
               
                   
                 CCTTTCCCTCATATCACTCTGTATCCACTTCCATTACGTAGCTCTA 
               
               
                   
               
               
                 BICFG630J358084 
                 ATTGTAAGCTTGCTAACCGAAGTGAGCCATCCTTCTCAGGAGAG 
               
               
                 (SEQ ID NO: 172) 
                 GGAAGACAGCAAGNAGGCCTTGCCTGAGGGAAATAAACTTAGT 
               
               
                   
                 CCAGTAGGTACGTTCTGTGAGAATTTGGCAACCCTCTGATGTGG 
               
               
                   
                 ACACACAGTGCCTATGGACGAAGTACTTAGTGACAGTTGCATAA 
               
               
                   
                 GTGATTGGAAAAGGCACTCCACACC [A/T] GTCCACCCACAGAGG 
               
               
                   
                 CCCCTTGGGCTTCCAGTTTCTACTCTGCTTTGGAGAAGAGATTCC 
               
               
                   
                 ACTGTGGTGAGACATGTCCATTCAGGGGACCTATTNTCATGCAT 
               
               
                   
                 CTCTCCACTTGTGGGGAGTTGAAATGGCCATGGTCTTTAGACCT 
               
               
                   
                 GGAGATCATCCAGGGACAATTTCTCATCATCAACTGGACTCCTT 
               
               
                   
                 CTACCATT 
               
               
                   
               
               
                 BICFG630J373954 
                 cttactttcaattatgaagaaaacacattcccttttcggtttaatccagtttcacccaatcacttttaatccaaaga 
               
               
                 (SEQ ID NO: 173) 
                 aatttggctaatacaTATCATTGCCACCACCACACATTATAAATGTGTAAG 
               
               
                   
                 TATTTACAGCCACTTTTCAGGAAGAGATTTGTTTTCAAGGAAATT 
               
               
                   
                 TCTCTTGCTAGCTTTCCAAATGTTTAA [C/T] ACCTACTTTGAAAA 
               
               
                   
                 GTAAGAGACAGAAAGTGCAACCTGCTCTTGCAAATGTGCCCTCT 
               
               
                   
                 GACATGCTAAGCCTAGTCAGTCCCAGTAGAATCAGCTAATCAGA 
               
               
                   
                 ACTGTGCAAGACCTGGTCCTCAGTTGCCAGGAGGGAAGGGGTA 
               
               
                   
                 GTTTGCCTTCACAACTCACGGAAATAGGGCAGTAGAAATTGACG 
               
               
                   
                 AGGCCTTAGGT 
               
               
                   
               
               
                 BICFG630J391832 
                 TCAGGCCACCCCTGCAAGCACCTGTCTCCCCCTCTGATTTAAGCG 
               
               
                 (SEQ ID NO: 174) 
                 ACACGCTTTCAATCCCACCCCACGGAGGGCCTCCCGTACTTTCA 
               
               
                   
                 AGCGAGAGCTGGCNGGATGCCTCTCTTCTTTCCTTGGATTTCCCA 
               
               
                   
                 CCTTCCTCGCCTCCGCAGATCCCCGAGCATCTCAGGCTGCTGGCC 
               
               
                   
                 ACCACTGAGTCACCAGGAGTA [T/C] AGCACTGCAGTACCTAGCT 
               
               
                   
                 CTACCCGCCATCAACTACCGCCAGAGTCAGAGCACTGCACCCCG 
               
               
                   
                 CACTTCCCACCCCACAGGAAAGCTCGGCTGTCCTACGGGGCTGG 
               
               
                   
                 ACCAAAAGGGGGNAAAAAATGTTTTTGTACTTCTAATGGCTCCC 
               
               
                   
                 CTCTGAACCAGTGCAGCTGAAATCCCCACAGTTCTAGAGAACGA 
               
               
                   
                 GGTNC 
               
               
                   
               
               
                 BICFG630J402866 
                 TGCTGGAGCGTCAGGCAGGAGAGGCCCAGTGGTTAGAGACACA 
               
               
                 (SEQ ID NO: 175) 
                 AAGCAGGCTGTGCCCCAAGCCTCCTGCTCCCCTGTCTGCCTCCTG 
               
               
                   
                 AGCCAGGCTTTTCNTGCCCCGGCCCCCAGGCCTTGTCGTGACTCC 
               
               
                   
                 CCGTGTGCCTCCCNGCGTCACCACCAGGCAGGAGGGAGCAGAT 
               
               
                   
                 ACTGTGTGGGNGGCCCTGCCGAGC [G/A] GCTCCCTGAGCTGTGG 
               
               
                   
                 CCATGAAGCACAATGTGCTTGCTGCCCTCAGGAGGCTTCTGGCC 
               
               
                   
                 TCTAAGAGAGCAGCCAGTGGGACTGCAGGAGCAAGGAGGAGCT 
               
               
                   
                 TTGAACTGATCAGGGGTAGGGGTTATGATGGGGCAAGTGGGGT 
               
               
                   
                 GGGGAGCAGTCTTCAGCCAGGTGTGCAGGGAGGGCCTCTCTCTG 
               
               
                   
                 GGGAGGAGAC 
               
               
                   
               
               
                 BICFG630J399661 
                 TCCTCCCAACAGTTGTCCAGCAGCCCCTCGGAGCCCCGTGGCTG 
               
               
                 (SEQ ID NO: 176) 
                 CCTGTGGAGCTTCCCTGGCCCTTTCCTCTACGTGCCTCCTCGTCC 
               
               
                   
                 CCGATTACTTGGAGTTTGGTGGGAGAGACAACTCTACCCAGGGC 
               
               
                   
                 TTCTGCGGCTGCTCAACAGGCACTGGAGGGACAGCTGGGGACTC 
               
               
                   
                 GGAGGGACCNAGAGTCACGTGCA [C/G] GGTGGCNAGTGAGATG 
               
               
                   
                 GAATCTAAGAGCTCTTCGGGCTCACAGCCTTTCCGCTCTGCAGG 
               
               
                   
                 AGGGAAGGCCTGTCTCTCGGGGCAGCAACCAGCCATCCCGCAGT 
               
               
                   
                 CCCNGGCTCTCCCTCCCTTACAACGCCCAGGAGGCTCCAGTGGG 
               
               
                   
                 TGCTTGGGCTCTGAAACGCTGTTCTTCCCCCCTCTTTACACCCCC 
               
               
                   
                 CCTCCCC 
               
               
                   
               
               
                 BICFG630J414309 
                 TGTTCCATTACCTTTCTTTTTGATCCCTGTGGCTCAGCCAAGTTA 
               
               
                 (SEQ ID NO: 177) 
                 AAAGAGTGAGAAACATGGACCTTTTTTGTCTCTGTTCCGGCTGTC 
               
               
                   
                 CAAGGACAAGGCCTGTTCCTGGGATGCGAAAGCTCTGAGGCAGT 
               
               
                   
                 CAAGGCTGACTTCTCCTTCCTCCTTTATCTTGCCAGAGGCTGGCC 
               
               
                   
                 CAACTGTGTCTCTCCCACCTT [A/G] TGAGCTCATGTACCCCTTGG 
               
               
                   
                 GCTCTCGCTACTCCCAACCCAGTATTCAAATTTGTTTTTTTGGGG 
               
               
                   
                 TTTGCTTATTTTGCTTTGTTTTAATATGGAAGGNGGTGCTCTCCC 
               
               
                   
                 TACTATGCCAGAGTTGTCCTTGNNGATGGGGGCGAGACTACACT 
               
               
                   
                 TGACCTCTTGACCTACTGTGANCACTTTGCAGAGTCTCTGGTCCTT 
               
               
                   
               
               
                 BICFG630J421119 
                 CAGACTAAGTAGATACATAAAGAAATAGAGTTCTATTCTTTTAC 
               
               
                 (SEQ ID NO: 178) 
                 TTACTATGACACATGGCCTGCTAGGGAAATACGAATTTAACTTA 
               
               
                   
                 AACCCGAGTGACAAGAGGAATATAGCAAAATATGGGCAGTTGA 
               
               
                   
                 ATAATGATATACTTTAGGAAATCTGTAAAACATTAAGAACTGTC 
               
               
                   
                 TTATTTATGGAAATCCTATACAGTT [T/G] TCCAGAGTCTTGAATG 
               
               
                   
                 TAGATCTGTTTTAAAAGGAACTCGTGGACCATTTCAACGTCGTTT 
               
               
                   
                 CTAGTGGCCTGACTTGTCACAAGTCATTTCATTCCTGGGGAACCC 
               
               
                   
                 CTGGTGGCAATTAGAAGTGAGGGAATTGGATAAGGAAAAATTA 
               
               
                   
                 AGCCTAATTAAAATCCTTGTACAAGAAGTCAGGAAAGAATAAA 
               
               
                   
                 CAAACTTG 
               
               
                   
               
               
                 BICFG630J431948 
                 TGTTGCTTGAAGATATCTGGAAGTGTAACAAGTTTAAATAAAAT 
               
               
                 (SEQ ID NO: 179) 
                 GAGTTCTGTTGGAATTAGGAAAAAGAATAAAATGTCTNTGTTGA 
               
               
                   
                 GTAAAACATTTTTAAGAGGGGCTACTTTCCTCTTTCTGTGTGCAT 
               
               
                   
                 TGTTATTAATTGCTCAGGAAAATGGTTCCATGTAAAAATCTCAT 
               
               
                   
                 GTGTAACTATTNCTTATCTATAT [C/T] TCACAATCTAATACACTTT 
               
               
                   
                 TCTAGAAAGTCTTAAGTTAATTTTTTGTTTCGATGCCTAAATTAA 
               
               
                   
                 AATAAAAGATTAAAACCTTGGATGCAATTAGCAAACATTTTTGT 
               
               
                   
                 AGTTGTGCAGAGTAAATTAAAGGACATTTGTGTGCTTATTTTCA 
               
               
                   
                 ANTCTAATGGAGAGCAAATTACATTNTtttttgtttttttNaaattta 
               
               
                   
               
               
                 BICFG630J425382 
                 AGTGCCGGGAAGGAAATCAAAAAACAAATGTGGCTGGCTTGGG 
               
               
                 (SEQ ID NO: 180) 
                 ACACTTAGATTTCTGAAAGCTATAAATGTGAAGTCTCATGTTTTC 
               
               
                   
                 AGCCTTAATAGCCAGAGGACTTTGCCTTGTGTTCAAACAATGTT 
               
               
                   
                 CTAGCTAATAGAATCACAGTCGTGGAAAAAGACTTCAAGAAGTT 
               
               
                   
                 CTGATAGTTCTTTTTCTGCCTCGT [T/C] CAAGAACAGATCGCAAT 
               
               
                   
                 TCAGTCTAATGGGAAGGTCATGCTCATTTAGACAGACTAATTTTT 
               
               
                   
                 AAAGGCCACTATGAAATTATTTTCTTTATGATCATTAAACAAAA 
               
               
                   
                 ATATTCGAAAATCAAAGAAAAAGCTGAATGATTCTGGTCTCCTG 
               
               
                   
                 CAGGACTGTGGGCTGTGGAACTGTGGTCAAAGATCACTACAGTG 
               
               
                   
                 ACCTTT 
               
               
                   
               
               
                 BICFG630J457850 
                 ccatagggagcctgcttctccctctgcctgtgtctctgcccctctgtgtgtgtctctcatgaataaataaaaagt 
               
               
                 (SEQ ID NO: 181) 
                 aaatcttaaaaaaaaaAAAAAAAAAAAGCCCACAGTGGTAAATACATACA 
               
               
                   
                 CAAGAATGATCTGGCCNCTCAGGCCACCAGGATAGAAGGTGCC 
               
               
                   
                 CTGTGCTCTGGACAGTTTCACGAAGCCATTGT [G/T] TAGACTGCT 
               
               
                   
                 CTATAGAACACATAGACCTGTGCAGCCCCCTTCTCTCCTCACAA 
               
               
                   
                 AAAGCCAAAACAAAACAAACCCTCtcaaataaggtcaggaaacttttgcctaagca 
               
               
                   
                 aaatttaaaaagattatttcaaagcacaaaactcaagaggctttaatatgccaatctgcactgtgaatttctaag 
               
               
                   
                 aagCAGTAGACTGTTG 
               
               
                   
               
               
                 BICFG630J473226 
                 AATCCCCCAGAGCAGCAGTTCCAATCGATGAGGAGCTGGCAAC 
               
               
                 (SEQ ID NO: 182) 
                 ATCCGGGCTGGGCATGGAAAGGTGAACAAACATTGTCCATTACC 
               
               
                   
                 CTGCCAATCGCCAGTCCCCTAATTCTGTTATTTTTTTTCCTTGGGT 
               
               
                   
                 AAATTTGGTTTCCTGAAATTAATTATTCAACAGGAGGTGACAGC 
               
               
                   
                 CGGTGTGTAGCAGCACTGTTGGA [A/G] CAGAGAATAAAGAGGCA 
               
               
                   
                 CATTGGACACAGCAGCTGCACCTCCCAGACCCTGAAATTTAAGA 
               
               
                   
                 TCTTTATAAATGATCTGTTAAAACTATAGTGACGATAAGCTTATG 
               
               
                   
                 AATCATGATCTATATTAATCAGGGCTGCTGATATGGAAAGATTA 
               
               
                   
                 ATTGAAACGTGCAGTTCTACACAAATGATAAAGTGGTAACAATT 
               
               
                   
                 TAATAT 
               
               
                   
               
               
                 BICFG630J484553 
                 AGGAAGCCGTGAGATTAGAACATAAGCTTCTGCATCCAGGGGA 
               
               
                 (SEQ ID NO: 183) 
                 AATTTCCACAGAGGGAAATTGTGGCCCTGGTGCTCACTTATACC 
               
               
                   
                 TGATTCTTGCCTCTCTTTCACACGGGAATCATGGGTTGGGTTTGA 
               
               
                   
                 AAAAACTGCAGAACTGTATAAAACCTCTCCTTCCTTTCTTTTTCA 
               
               
                   
                 AGCTAGGAGAACACAGTGTTCAC [A/C] ATATAGCCTCCCACTCA 
               
               
                   
                 CTTCACAGAATTGACAAGGGAAAACAGTGTGCTTGTGGGCCTGA 
               
               
                   
                 GCACGACTTAAGCAGGGTGAAGTCTGGGACAAGACTGCACCAG 
               
               
                   
                 GATCCTTCCTCCCCTCCCTTTAGGTTCTTTGCCTATAGGATTCTA 
               
               
                   
                 AAGGCTCAAGGCCATGGGGGCAGTGACACTTGCTTAGGGAGAC 
               
               
                   
                 CCAGCCAC 
               
               
                   
               
               
                 BICFG630J497958 
                 GGTTGATAAAATCAGGGCTTCATTGTCTTTTGCCAGCCTCAGTTT 
               
               
                 (SEQ ID NO: 184) 
                 GGCCACTTGAGAAATGACAACATTGGACCAAATAATGAATTTCT 
               
               
                   
                 GATGCTTCTAGAGTCTGTGATTTCCACATGCTGTGACTGTAAGA 
               
               
                   
                 GCAGAGTCATCAAGGCTTGGTTTTCTGACAAACAATTCCAGGGA 
               
               
                   
                 AATAGAGCTGGTGGGGGAGGGGA [T/C] CCCAGCGCTCACCCCAC 
               
               
                   
                 CGCAGCCCCCACAGAGGGCTTCCCGAGCTGCCACCCAGCTGGTT 
               
               
                   
                 GACCCCCAAAGGAGCAATTTGCACTTTCTGCTTTCCTGGCCTAA 
               
               
                   
                 GATAAAAATACCCCTGTCACATTGGATTAGCATCTCCCCTTTCNC 
               
               
                   
                 TGAGAATCTTCTCACGGATGCAGCCCCCTTGCTTTGTCAATATTT 
               
               
                   
                 TCAGA 
               
               
                   
               
               
                 BICFG630J503647 
                 AAACTAAATGCTCACAANGGCAAAAAGCAATGTGAGNNGACCT 
               
               
                 (SEQ ID NO: 185) 
                 TGCAGGGGCAGGGCGAGTCATGGAGCGATTGAAAAAGAAAGAA 
               
               
                   
                 AAAAAAGCAACAATTTTTAATAGAATTCNGAAAGTCTGCTGCCT 
               
               
                   
                 CGTCTGGTTTACAAATAGGCATTGTTNGAGGAGACAGAATAAAT 
               
               
                   
                 AAGAGCTAACTACAGCATGNATTACC [A/C] AACACTAANCCCAT 
               
               
                   
                 CAACGAGTCCCGGTGGCAGCACAGATCACTCAGGCACGCCTTGG 
               
               
                   
                 TCACTCTCCNCATATTTATTTATTAAGAAGACAGTGGAGTCTGGC 
               
               
                   
                 TAATGCGATACAAAATTAATATCANCTGTAAAGAAACATAACCC 
               
               
                   
                 ATACATTCAAAGCGATAACTCTACCGACACCCTCCCCCCCAACT 
               
               
                   
                 CAATCAAGT 
               
               
                   
               
               
                 BICFG630J525153 
                 CTAGAGCCAGGAATGTTCCGATGTCACCGGCAACTCACGGTACC 
               
               
                 (SEQ ID NO: 186) 
                 ACCACGTCCAAGGCTGCTTCCTATTCCACGTGCGGCAGAGGCTG 
               
               
                   
                 CCCGCCTGCCTCCCCCCTCCCAGGGGCTGCCTCCCCACCTCAGGT 
               
               
                   
                 GGCACCGTCCTCAGAACTGGGGCACAGAGGATGCAAGCCAAGC 
               
               
                   
                 TCATCAAATCCTCTCCCCGAGACC [A/G] CCGTCTGCTGTAGAACA 
               
               
                   
                 CNGCCGCCCAGAGACATGTACNAGAACCCCTAACCGGCTCGTGT 
               
               
                   
                 CGGCCCGTGTGTCTATGGAGGCGTCATGGATGAGCTCTTACACA 
               
               
                   
                 CTCGCCCGTGACTCCACCATCACAAAGTAGAAACAAACCAAGA 
               
               
                   
                 ACGCTGTAACGATGGAAAATCTACTGACCCTGACCCCCTACCCC 
               
               
                   
                 TCCCCGCT 
               
               
                   
               
               
                 BICFG630J533364 
                 GCNCAGGGACTGCCACCGAAGAGCCCTGAGCATCTTCTGACCAG 
               
               
                 (SEQ ID NO: 187) 
                 TCCAGGCTGATGTGGCTTCACTCCTGTTGCTGCACATCTCACCAT 
               
               
                   
                 TCTCTCTTGCTGTTGGCCAGTCTTCTGCCCTCACTTTGTGCTTTCC 
               
               
                   
                 CATGTCAACATGGAATTGGAGCTGCTCAGACTTGAGCTCAGGGT 
               
               
                   
                 ATCGATGGTCCTAGAAATGGA [A/G] TGTCAGGAACANGTGAGTG 
               
               
                   
                 GGTTTATGTGTTTTCCCCAGGAAATCAAGAACTGATGGTAAACA 
               
               
                   
                 GAAGCANGAAACACCATTTGAGTTACTGGCGTGTTAGTGGAAAA 
               
               
                   
                 CCAGTACATCACCCCTGCCAGTGCAGGTAGGTAGCTGACCCACA 
               
               
                   
                 TAGTTCCGTATCACCTTCTTGTTGAAATTAGTATCTTGCATCTAT 
               
               
                   
                 TCTT 
               
               
                   
               
               
                 BICFG630J537466 
                 ACAGAACATGGCTCCTCACATGGGANCAGCCTCACTNACCCACA 
               
               
                 (SEQ ID NO: 188) 
                 ACATTTCAGATAAGGAGGAAGTGAGACAAAAACCCCTGGGTCC 
               
               
                   
                 TTTTATCTGGTCCTCTTCATTGAGAAGCTTCTGTGGAGCTTACAG 
               
               
                   
                 TCAGGATCAGATCTGGTGTTGACCAGCAGGCTCTTACCATGGAG 
               
               
                   
                 AAGGTCCACAGGGAGTTACCAGTG [G/A] TGGCAGGGTCACTTGT 
               
               
                   
                 TGCCTATCCTGGGACCTGCAGCTGTCAAGTTCCGGAATNATTCTT 
               
               
                   
                 TTTCNTTCCTGGTTCCTGCCCTGAGACCCTCATGAGAGGCTCTGA 
               
               
                   
                 GTTGTGTGTTTCACACAGAATAAGAGGTGGCTTTGACGTCCAGT 
               
               
                   
                 CCCCTAGATCTGTCAGCCATGAGGTTTGCACATGCACATTTACA 
               
               
                   
                 CGTGTC 
               
               
                   
               
               
                 BICFG630J548189 
                 GTACGCACACACGTATATACGTACACACGCATACGTGTATATAA 
               
               
                 (SEQ ID NO: 189) 
                 CACGCAACAGACNTATGAAGACCCGCACAGAGATTAGAACGCG 
               
               
                   
                 ATTAGAGATCAGAGAGCGAACCTCAAGGGGCCTGGCCCGGAAA 
               
               
                   
                 CTAATANATGTGGAAAGTCACTGAGGGGCTGACGGAGGCTGGA 
               
               
                   
                 CNTTCAGACGAAAGCCAGACTAAAGGG [A/G] CGAGAACTGGTCT 
               
               
                   
                 GTAAGCAGCCTCTATGTAACGGTGCCCGGACCAGCCCTGCCGAT 
               
               
                   
                 GACTGGACACCCCAACTTCCGCCGAAGGCCAGACAGTCACGCCG 
               
               
                   
                 ACAGGAGTGGAGAGGGTTTTAAATCCCCCAGAGAAAAAAGAGC 
               
               
                   
                 TGAAAGCCCCAGGTAGGGCAAGNGGGAGAGAACGAGGCCACGG 
               
               
                   
                 GGGCAGCCACACA 
               
               
                   
               
               
                 BICFG630J553154 
                 TCCAGAGGGCAGCGGCAACACANGGTGAGTCTCCAGGGGTGTG 
               
               
                 (SEQ ID NO: 190) 
                 GCACGGGAGACCCCACAGACCAAGACCACGGCGGGCTCCGCCT 
               
               
                   
                 TANNGCATCAATCCNCNGAGAGCGGTCCCGNGCCACGTTGCCTC 
               
               
                   
                 TCTGGAGCATGATGCAAAGGCANACGNCTGCTGCCNGAGACTCT 
               
               
                   
                 CAGGGCTGACAAGTCTCCAGCCAAGG [G/C] CTCACATGTCCTTG 
               
               
                   
                 GCNTGTCAACCGTGATCGCGAGCAGCAGCNTGGCCCGATGCCCG 
               
               
                   
                 TCNCTTCNTTGTGGNAGCCAGACTGGCTTCGCAACTCNACTCAC 
               
               
                   
                 CTGCTACGCGCCCAGGACTGNTGAAGCCGGGGCCCCCTGCGGTC 
               
               
                   
                 CTGCCCACNCTGCNAGCTTCCAGGGTCGTGGCAANCGGACTCCC 
               
               
                   
                 GCCAACACCT 
               
               
                   
               
               
                 BICFG630J566667 
                 AGCCTTCAGAGGGGGCTCCGGCCATCAGGTGGGCCGCAAATCTC 
               
               
                 (SEQ ID NO: 191) 
                 CGCACAATGGGATGGTAATGTCTCTGAAGGACAAACAGGACAA 
               
               
                   
                 CCACATTTTGCACGTCAGTAATTTATAAAAGACCAACCACAGCA 
               
               
                   
                 AAGATATTCTTTAATAAACACTATTTCTTAAAATCACAGATACGT 
               
               
                   
                 ATGTAATTCACAGTAATCACATAC [T/C] GAAAGAAAAAATACTC 
               
               
                   
                 TTTGCTCACATGATTACATTAATCTGATGACCAAGTTACCAGTGA 
               
               
                   
                 CATAGCTTAGTCTAGCTTTTACAGTATGAAAAGAGTAATCTAAA 
               
               
                   
                 AGCAATTTCTCTTTTAGAGTGGAAAAAGTTAGCTTACAACAGGT 
               
               
                   
                 TTCCTGAGACATATCTGCTATAAGTCTCCCTCTATGTCCACACTC 
               
               
                   
                 AAGGAT 
               
               
                   
               
               
                 BICFG630J573029 
                 TTTGGTAAATCTCANATTTTGACATTTTATANTGCCTAAATTCCA 
               
               
                 (SEQ ID NO: 192) 
                 AGCTGTCTGTGtttttNttttttNtttttttttCTGGTCCTAAAATACGTAATTCT 
               
               
                   
                 CCAACTCAGTTTTTCAACCTCCAGAAATATTTTAGCTCTCCTCCT 
               
               
                   
                 CCTCTTCATCCTTCTCTTCCACATCTCTAATCCTCATGTCTGTGTT 
               
               
                   
                 GCTCTTGA [T/C] CTTGACTTTGGAGCCAACCAATTCACTCTTTAG 
               
               
                   
                 CCAACACTGGGCTGGNCCAGAGAAGGGAGCGTGGATAAAAAAC 
               
               
                   
                 TAATGAATATTGTCAGTTATCCACATTCTGAAATTAATGGAATTA 
               
               
                   
                 TTGCCAACAACTTGGAAGACCTGCCAGAGAGGGAAAGTGACAG 
               
               
                   
                 ACTCCTGGCAAAGACAATGATAGGATGAGCAGTCTT 
               
               
                   
               
               
                 BICFG630J585149 
                 GTGACCAAGCACTGTGGTGTGCCCCTCACTCCTTTATCGTCCGTT 
               
               
                 (SEQ ID NO: 193) 
                 GACTCTAAGCATCAGGACATCATGGAAAAGACGTGTGAGATTCC 
               
               
                   
                 TCTGCCCTTTCCCCTCTGCTATTCTCTGATAATTTTTCCTCCTCCC 
               
               
                   
                 AAACTGCCTGGGAGCCCTGCTTCGCTCTGCTACACATCCTGGCC 
               
               
                   
                 ACAAAGGAAAAGCAAGACTTG [G/C] AGAGTGTGGTTATCTGGGG 
               
               
                   
                 TCCTTTCCCCCTGGCTTTTTCTCCCTGGCCTGTCAACGATGCAGG 
               
               
                   
                 CCTGGGGATTTTCAGCCTGGGCTATGCCATGGACTCNGAGCTAA 
               
               
                   
                 AATGCTTTCCATGGCTGAGGCTCAGAAAGCAGGTAAGAAGTCCT 
               
               
                   
                 GGTTTAGAGGCAAAATCTTCTTTTCTCATCCACAGAAAGCCCCCT 
               
               
                   
                 TGT 
               
               
                   
               
               
                 BICFG630J597522 
                 tttAACTAAAGCTCCAGTCCCCCATCCCCNTCCCCCATCCCTGCCC 
               
               
                 (SEQ ID NO: 194) 
                 CGGAGCCTCCAGCATCTAACCGGCATTTACGAAGAAGAGGTGG 
               
               
                   
                 ACGGTCGCTCCTCCCCTCGGATAGTGTGGGTTTAGGGCTTCGGG 
               
               
                   
                 GTCCAGTACANCACGGCCTGCACGCAGTCTGGCTCTCTCAGGGC 
               
               
                   
                 CTTGCGACCTGATCTGGGCCTGT [T/C] GTCATCACTGCACATCCC 
               
               
                   
                 CCGGGCGCCCACCTGGCAGGTGGCAGGCTCCCCCNGCAGGTGGC 
               
               
                   
                 AGGCTCACCTGGCAGGTGACAGGTGACGTGCTGCCCACCTGGGC 
               
               
                   
                 GCGGCAGGTGGAGGAGCACCCAGCACCATCCCCGTAAGTGGGC 
               
               
                   
                 GCAGTCGGCCCTGGGGTTTCGCGGGGCCAGTGACTCAGCCAGTG 
               
               
                   
                 GCCACTC 
               
               
                   
               
               
                 BICFG630J608671 
                 GAAATGGTAGTAAAAGGGTGCACGCCTTATAATTTAGGCCAGGC 
               
               
                 (SEQ ID NO: 195) 
                 CTGCATGACCTCAAAGCACCCAAGCAACTCATTGAACAGAAGA 
               
               
                   
                 ATCAATCAAGTTTGATACCGGTTGACAAACGAATAATAGGACAA 
               
               
                   
                 GATCATTTGCTTGCTTTGCCAGTGCCCCCCAGGTGTGCTCATGGG 
               
               
                   
                 GCATGAGCTTTAGTGCAAGTGCCA [G/A] CGAAAGAACCTGTATT 
               
               
                   
                 TCCTGTTAGCCTGGTGGTCTCTTCAGAGGGCAAACTTCAATAAT 
               
               
                   
                 ACTGATGGTGTGGATTACTCGAATTTGCCATTTGCTACTTGACAC 
               
               
                   
                 AGTACCCTTAAAATAGCCCGTCAGCCAAGCAGCCGTGATTGTGT 
               
               
                   
                 TTCTCATGCCACCGTCCTTTGCAAAGTAGGTTTGTGGATGGTATT 
               
               
                   
                 TCGGGG 
               
               
                   
               
               
                 BICFG630J613547 
                 TTTTCTTTCCTCAAATTCCTACAAAGGCCAGACTTATTTTACCAG 
               
               
                 (SEQ ID NO: 196) 
                 GATGCCTTCCAATTGAACTGGCTATAGGCCCAGCCTTTCAAGAA 
               
               
                   
                 ACCAACAGCAAATGCTGGCCTCTGAAGAGGTCAGTATTGAAAG 
               
               
                   
                 GTAAATCTTATATTCACCTAGGACTTCTAGGGTGTTGTCCTCTCA 
               
               
                   
                 GAGGCCAGAATTACCTCTCAGGG [C/T] CATGAGAATGGCCTTTTG 
               
               
                   
                 GGGACCAGGATTCTGATGGCAAGAGCCTGGGCTCCAATGAGCTT 
               
               
                   
                 CAAACTGATCTTCTTTTCTATCCATTAACCACTGGTATTTCTGAA 
               
               
                   
                 AGTCAGCCCTGAATTTCTAATCTCTACTTGGGAGTTAATTACCAC 
               
               
                   
                 CTATAAAGACAGTGGCTGCAAAAAAAAAAAAATCTCTATTTTCC 
               
               
                   
                 ACCC 
               
               
                   
               
               
                 BICFG630J630348 
                 GGAGGGGATAAGGCAACCCCCTTGGGCCCTACTTTGGAGAAGA 
               
               
                 (SEQ ID NO: 197) 
                 CTTTGTAGAGATGAACAACTGTCCGCAGGCCTAGGATGCAAGTG 
               
               
                   
                 TCAAGGCCGGACCTTCCCTGGGTTCCTCAGCCAGGTCCACTGTG 
               
               
                   
                 GAGTCTCCCACGCATGGCCTTAAATGGCCACGCCCGGGCCTGAC 
               
               
                   
                 AGGGCAGTCACGATCCCGGAACTAC [A/G] GTCACTCGGCTTTAA 
               
               
                   
                 GAAGCCCATTGTAAGCCTGACCACCAGGGAAGAGTTGGCCAAA 
               
               
                   
                 CTCCATCCCAAGACTGGACGGTAGCCCGGGAGATTAAATCCTAA 
               
               
                   
                 ATAAATACTCCAACTAAATGCCTTGACTAAGAAGCCATGCTGGT 
               
               
                   
                 CTCTAGTTGGAAATAAGGCAAGAAAGAGCNGTGATAACATCAA 
               
               
                   
                 CCACACAAGGG 
               
               
                   
               
               
                 BICFG630J635046 
                 TGGTGACCCNGTGGNGGGGAGGCGGGCGGGGAGGCAGGTGGGG 
               
               
                 (SEQ ID NO: 198) 
                 GTCCATGTGGGCACTTCCCGGCTTGGGCCTGTCCTTCAGCGGGA 
               
               
                   
                 GCAGAGACCAGAGCCGNGCCGGGGGCCACGCGGAGCCTCACCG 
               
               
                   
                 GGGTGGGCGTCCGGGATCCCGCCTAGGAGGGGGTTGGCGGGCA 
               
               
                   
                 GGGCCCATCCCNGCCCTGCCCCGTGCC [G/A] CTCGCCCGGGTTCT 
               
               
                   
                 GCAGGGCCTGGCGCTCATTTCTGCGCCTCTGCGGCAGCGGAGCT 
               
               
                   
                 CCCCGAGCCCCAACCGTGGTGTCTCCGGAGCCCCCNCGGGAGAC 
               
               
                   
                 ACGACCACGTTCTCCCGGGAGCACCTACAGNGGCCCCCNNAGG 
               
               
                   
                 AGCAGCCCTTCCAGCTTGGTGTCTGGGCTCCGTGCCCTTGCACCA 
               
               
                   
                 GAAGTTTCCA 
               
               
                   
               
               
                 BICFG630J638804 
                 TCTTGAAACATGGACAAGGCAAAATCAAGAACAAAATCATCCTT 
               
               
                 (SEQ ID NO: 199) 
                 TTGACAGACAACACAGTAATTGAGAAGCACCTAGAGAAGTATG 
               
               
                   
                 GTGTTATGTGCTGGAAGACCTCATTCATGAAATTGCCTTTCTGGG 
               
               
                   
                 GAAGAATTTCCAGGCAATTTCTGAGTTCTTAATGCCTTTCCATCT 
               
               
                   
                 CTCATGCTACCAAGAAGAGAGTG [G/A] GCCTTCTCAAAGAGGTG 
               
               
                   
                 GGCTTACTTGGCTATGGAGATGAATGCACCAATCAACTCATTTG 
               
               
                   
                 GCTGCTGAACTAAACTGAGAACTCTGAAAGCACAGTGCAGTGG 
               
               
                   
                 AGGCATGTGTTTTGTTTTTTGGAATTGTTATCCAGTATCTTCAGA 
               
               
                   
                 AAAGATTATTTTCTGCTATATCTTCAACAACTAGATAGAAGGGT 
               
               
                   
                 CAGGAAA 
               
               
                   
               
               
                 BICFG630J636447 
                 AGGAAACTGACACCACTGATACCCCCTGGGGTTGGCATGCACCT 
               
               
                 (SEQ ID NO: 200) 
                 CAAAACTTatagataaatacataaataagtaaataaataaataaCaattaaaataataaaataaaaaa 
               
               
                   
                 GAAATTTCTAGCAAAGATAGTTTAGAAGTAATATGTGTCTTTCA 
               
               
                   
                 TATTACTAATTCTCCCCACAAAGGAGAGCTATTTCTTTTAACTG 
               
               
                   
                   [G/A] TCAATACAACCTCAGTTATTTCACATCTTNACCTCACATAAA 
               
               
                   
                 TTTTTTTTAATGTCATATTGTATTATTTTGAATCCTGTTTNCATGT 
               
               
                   
                 AAGTTTTGTGTGTNTTCCCAAGTAAACTGTAAATTTTCTTCTCTA 
               
               
                   
                 TATTGTGGGTGCCAACCCTGAAACCTGACNGGAGTGGTCCTTCT 
               
               
                   
                 TTGTGNGCCTAGGCCTTCTAGCT 
               
               
                   
               
               
                 BICFG630J654194 
                 gatcgtaggatagttttatttttagttttttgaggaacctccctgctcttctccagagtggctgctctagtttgcatt 
               
               
                 (SEQ ID NO: 201) 
                 cccatcaacggttcaagagggtatgcctttatccgAGGATGTTAATTTCTTGCTCTCA 
               
               
                   
                 ATTTTGTTATCATTTGGAAACCATTTCTTTGAGTAACTTAATGGT 
               
               
                   
                 TAGGGGTGCAGAGGGAAGA [A/G] CAATTACTTTGCTGACTGGAT 
               
               
                   
                 GAAGTGTTTTGAGGCAGCGTGTACATACCTATGATGGGCAGCGT 
               
               
                   
                 GACAACGGACACCTCAGGGATGCTGATTTTTCCAGTTGGTGAGT 
               
               
                   
                 GAGTNTGTCTTCATGTTTGAATGAACTGGGCTGGCATGCAGAGT 
               
               
                   
                 ATATAGCANTATGACTAGTCTCAGTTTATTGGTAATGTGAATGT 
               
               
                   
                 AAG 
               
               
                   
               
               
                 BICFG630J660369 
                 TGCTGGCCTCCCTTGCTGGTGTCCCTTGTCTCCTAGCTCACCCTC 
               
               
                 (SEQ ID NO: 202) 
                 ACCTCCTATGTGGTTTGTCATACTGGCCCCCTCTCCAGCCCCCGC 
               
               
                   
                 CCTGCCCTTGTGGCCTAATTCCTTGGCAGAGCTCCTGGCTGACCA 
               
               
                   
                 GACACTTGCATGGCTGTTGATTCCTTTGGCTGGATTCCCTTAATC 
               
               
                   
                 CCTGCAAGCTCTCTTCCTCG [C/T] TTCAGACCCTCAGCAGAACCC 
               
               
                   
                 GGACTCAACTTCTGGAAACAACCAAATGGTAGCTCTCAGCTCCA 
               
               
                   
                 TGGCGGTACATTCTTGACAAAGGCCACATTTTTCTATGATTGGG 
               
               
                   
                 AGCTCCAGCCTTAGGGCTGTGCAGTCCCCTAAAGTCATGATTTCT 
               
               
                   
                 GGGCCCTGCCAGTCAGCCATCTGCAGGGAGGTAATTtatcagttac 
               
               
                   
               
               
                 BICFG630J667882 
                 GTGTCTGCGTGTGTGTCCGTGTCTCTGTTTCTCGGTCAGTGTGCG 
               
               
                 (SEQ ID NO: 203) 
                 TGGACACACCACATGATTTCAGTTCAGTCGGGGTCAGGCTGGAG 
               
               
                   
                 AGTCAGGGCCTCCTGTCTACGTTCAGTTCTNCGACTGANGGCAG 
               
               
                   
                 GTTTCCTAGGGCCCAGGTCAGGGCGACCCACTGGGGGCACCGCC 
               
               
                   
                 GTNCACAGCTCACCCAGGGGAAA [G/C] GAGGGAAGGGCCCGGA 
               
               
                   
                 ACGCACGACAGGCAGGGGTTCNGGATGAGGACGGCCTCACCCC 
               
               
                   
                 TTCTAGGCCAAGACCCCGACACCTTCTGTAGGGTTTGCCTTGGAT 
               
               
                   
                 TCAGGAGCCTAGGATCGTGGGGAGCTATTGCCCATCCCTGCCCN 
               
               
                   
                 GGGGGGACTGTCATCTTCTGGGCCTCCAGAATGAGGGTACCGGT 
               
               
                   
                 GCCCNtgt 
               
               
                   
               
               
                 BICFG630J676160 
                 ATTTAAATAAGTTGCTCCCAATATTATAATAGACACAAAGTGAA 
               
               
                 (SEQ ID NO: 204) 
                 GCTTATTGGTAATTACTCATTATGAGACAAAATGATTAAGAACC 
               
               
                   
                 CCANGGAAAAGAAGAGAACAGATTTGAATCATTTTTTATGTATA 
               
               
                   
                 GGTAGGACATACTCCCAGGTTTTATGAGCAATGTGGCTTGAGGT 
               
               
                   
                 TACTAATACCTTAAGAAAATTAAG [T/C] GCTAAGTGTGTCTTAAC 
               
               
                   
                 TGCATCAATAATTTAAAGTGTCCTAAACACTATGGTAAAAATGC 
               
               
                   
                 TCCCAAGATTCCTACTCAAACATTTTGAGACCTTAAGCTAATAG 
               
               
                   
                 GTATTACTGTCCTCCCCCACTCCCTGCATAGAACACGGGCTACC 
               
               
                   
                 AGGCAGATGTCGAATCTGAAGAAAAGAGGATATTAGGGCCCAA 
               
               
                   
                 TAATCAGA 
               
               
                   
               
               
                 BICFG630J689381 
                 GCTAATTAATCCATCCCACTGGGAGGCACACTTATCATTAAAAG 
               
               
                 (SEQ ID NO: 205) 
                 GAGGCAGCTGATTTCAAAGCTTCATACCCTCCCTTCCCAGCTCA 
               
               
                   
                 GCTTTCTATTTGGTTTCCAGAGTAATACGGGTTGCCTGAGAGCTG 
               
               
                   
                 GGAAAAAAGAAACCTCATGTTCNTTTTCTGTAGTGGGTTTTGAT 
               
               
                   
                 CTTGTAGCTGCTTATTTCTTAAG [A/C] ATTAGGAAAAGGGTACTT 
               
               
                   
                 TACAGCTGGAATGGGAGATTGTAGACTGGAATGTGTAAAAAGG 
               
               
                   
                 TGATATGAATCTTCAGGCTGCATTAGCTCTAGGAAGACCTCTCA 
               
               
                   
                 GTTTAAAGAATGATGTTCATCTTCAAGAGAGAGATTAGAAAGCC 
               
               
                   
                 NGTAGCTGTATTTGCTTGAGGATGCAAGTGAGATTCAGTGATCT 
               
               
                   
                 GGAAATG 
               
               
                   
               
               
                 BICFG630J678332 
                 GCAGCTGTCTCAAGCACAGCATGTGCATCCTTGGGCTGCAGGTG 
               
               
                 (SEQ ID NO: 206) 
                 ATTGTCACTGAAAGAAAAATCTTTAGCTTCCCTAAGTTAATAAA 
               
               
                   
                 CTTTTAACTAGGTTAAAAGGTAAACGAAGTGTGATGAGCACAGC 
               
               
                   
                 CAGGAGTCTGATCCCGTCAGAGACTTGTCAGGGCCTCCTGCCTG 
               
               
                   
                 GACAAGTGACAAGTCTGTGATTGG [A/T] TGGGACAGTCTGCCTG 
               
               
                   
                 CCCAGAAACCTAGCTGTAGGAAGTTAGTGCAAAATGGAAACCA 
               
               
                   
                 GTANAGGTATTGTATAATGACTCCATTGTGATTTAGAAATTCCN 
               
               
                   
                 GATCATTTCATATGACATTTCTTTTTAATCACTTAATGTGAATAT 
               
               
                   
                 ATAAAGAGTTTGACATTTGTTTAGATATTTTTCTCCTTTTGATGC 
               
               
                   
                 TATCTCT 
               
               
                   
               
               
                 BICFG630J693521 
                 ATTTACTCGAGCTTTAGTTCATTTCTTTTGTGTGTCATACAATTAT 
               
               
                 (SEQ ID NO: 207) 
                 TCTAGTTTGTCAGTTGGTAATTGACATTGAACAAGCTAGAGAGA 
               
               
                   
                 TTTTGGTTTTGGGGAGAAGTAAGGGAAACTGAGTATTTGTAAAG 
               
               
                   
                 AAATGCTTCAGTAGCTCGGGGCTCCCCAGCCTGTCCTGTCAAGA 
               
               
                   
                 ACTCTGTTATCTTTGCATCATC [A/C] TATCAGATAATACAGTCAT 
               
               
                   
                 CATTTTAATGCCAAATGTCACTTTTGTCTCTTTAAAGAAACTAAC 
               
               
                   
                 ATGTTGTTATCACTACTGATGTCAGATCAGCTGGTATTTATCTTC 
               
               
                   
                 AACTTGATAAAAATGTGCAGTGGTTCCCTGTTCTCACATTAAGA 
               
               
                   
                 CCCAGAAAGATTTAATGAAAAGTATTGTGTGGCAGCCTTACTTG 
               
               
                   
                 GTC 
               
               
                   
               
               
                 BICFG630J695147 
                 gtaagctctctctccctcatgaaaataaataaattaattaattaaattaaataaataaaaTAAAAATCTT 
               
               
                 (SEQ ID NO: 208) 
                 AAGAAAAAGATGTAAGGAATAAGTAGGAGAAAGATAAGCAAA 
               
               
                   
                 GAAGATAGTTCTAAGGTCCATAGTCTGGATTAGGACCCCATCTC 
               
               
                   
                 TATTTCTGTCTAGGACAGGATGCTAGCTACTGGGAGGCAATGGC 
               
               
                   
                   [T/C] TATTCTTTAGGTTACATAAGTGTATTTGTAAGCCCTTGAGGG 
               
               
                   
                 AGCACCAAAGACTAAGAACATTTTCTTGAGTACTGAAGTATAGC 
               
               
                   
                 CTAAGAGTCTTGGGTAAGCTTGTGCTACTCATGGAGAAATTAAA 
               
               
                   
                 AGGATACCACTTTCCACTCTCAACCTCCATGAAACCCCAAGAAA 
               
               
                   
                 CTAACATGAATATCCAAATCTGCTTC 
               
               
                   
               
               
                 BICFG630J707814 
                 AATAGCCCAGCAACTCCTGAGTGGATTAGGATGACTTGTGTGGA 
               
               
                 (SEQ ID NO: 209) 
                 TCAGTTTACTGACATGGTAAGAGTAGTGGAGGGATGTCCCAGCT 
               
               
                   
                 ATAACCACCGCTTAATGGATTTTCATGTTCTTAGTATGGATTTGG 
               
               
                   
                 TAATCATGACAAAATCACAGGGCAGGTTCATCTTTTCCAATGAA 
               
               
                   
                 AATTCTCACTCTGCCTNTATACT [G/T] CAAGCAACTGCAAACCTC 
               
               
                   
                 CTCAAATGATGGCTTGTTTCAGACTGCAAAGAAAAGCAGACTCA 
               
               
                   
                 GTTGAGCCANTGCTAGGAGCGAATCAACATGGTAGCTAACTTCT 
               
               
                   
                 TGAAACATTCTGTCAAAATGTAGTTGATGTGGTATTTTAATCACC 
               
               
                   
                 TTAATAACCAAATTAGATTAAATAGATATCTGATCTGGTCAATA 
               
               
                   
                 ATTCA 
               
               
                   
               
               
                 BICFG630J715531 
                 GAAGGTGACTGCCCCATGGGAGATGCTTGAGCTTCTCTCCCACG 
               
               
                 (SEQ ID NO: 210) 
                 TTACTAGGTCCCCCGGTGACAACAGCTATGGGTGCGGGGCATCT 
               
               
                   
                 CCCTTCACGGTGCTGCCACATGGGTGCCTCACTGGTCCCGTTGCC 
               
               
                   
                 TTGGGACAGAATCACTTTCTCTTGTTGTGGCAGCGGGGTTTGAG 
               
               
                   
                 GCAAAACCGAAGTAACAAGATGA [T/C] AAGGAGATGACCCGGG 
               
               
                   
                 CTCTTGTACCCTGACGGGGAGGGGCATGGCGGGTGGGTCTGCTC 
               
               
                   
                 TTGTCACCATGCAGGAGGAGCGAAGCACCACGCAGTTTCAAGA 
               
               
                   
                 GGCAGAACTCGCCTGTGCAAGAAGATGAGCTCTGTACAGTAGCC 
               
               
                   
                 CCGTCTCGGGATTAGACCAGATGACCCCGAAAGCCCCATCGTTA 
               
               
                   
                 GGGTTGAAG 
               
               
                   
               
               
                 BICFG630J719405 
                 ACAATAGTAACTACCTGCCCTGGTTGAGATAGAACATCTCTCAG 
               
               
                 (SEQ ID NO: 211) 
                 GGTGATGATTTTTTTTTTTTCAACACAATACAATAATTGAGGTAG 
               
               
                   
                 GAGCCTGAGTTTCAGATGGGTTCCTGTGTTGTGGGACCTGTGAT 
               
               
                   
                 AAAGCATATACCTTCCAATGATACTAGCATTTTCTAAAACGTGA 
               
               
                   
                 CTGGCCTCTTGAACCCTGTCTGA [A/G] GACAGGGACAGACCAAG 
               
               
                   
                 GACACGACACTGTCAATACGGACTCCTAATCCTGCCTGTTGCTC 
               
               
                   
                 CTACATACAGTGGCTTTATCTTCTCTTACACACAAGAGCCACCCC 
               
               
                   
                 TTTANTCTTCTGTTATCTTGAAAAGATACCTGAAAAGAGCCCTGC 
               
               
                   
                 CTCAGGATCTTGGACTTAAATCTCAGTTGTTCTTCCAACCATTTA 
               
               
                   
                 TGGG 
               
               
                   
               
               
                 BICFG630J724770 
                 AGTTCCCTCTTAGTCCCCTGAATGGACCATTCCTTGTTGTTGAAT 
               
               
                 (SEQ ID NO: 212) 
                 TAACTACTATGTGCCCTNGACTTTTCTAGCAGTCACAAAGGCAG 
               
               
                   
                 ACTGGGACATTTATTGGATCTTAGGTCTCTTATTAGCCAAGGTCC 
               
               
                   
                 TTTTCAGCCTAAGCTGTTGAACAAATCAGGTTACTACCTCAGCC 
               
               
                   
                 AGACAGAAATGGTGAGAGCTTA [A/G] TGCAGGCAGAAATGTTAG 
               
               
                   
                 TAGAAGTGCATACATTTCTGCCCTTAGCAGGATAGCACCAGCCT 
               
               
                   
                 TCTCTCTGGGATGTGAAGTNTAGGAGTAGACAGAGGAGGTGAG 
               
               
                   
                 AGCTGCTTCCTCCACCCCTGCAGGGAGGGTTGGAGAGCAATGAC 
               
               
                   
                 TCTCTGGTACTTACTCTCCTTGCAACGGCCTTGGGCTTCCTGGCT 
               
               
                   
                 TCCTTC 
               
               
                   
               
               
                 BICFG630J729876 
                 TCCCATGCTAACACTCACTGTGGTCATGTCAAATGCACGTACCC 
               
               
                 (SEQ ID NO: 213) 
                 CTTCCAGATTAAGTGTTGTAAGTCCCAACGCGTGGTTCCGCCTCA 
               
               
                   
                 AACTGTTGTTGAGGGCTTATGTGAGAAAATGAAGATATTAAAAT 
               
               
                   
                 CCATGTTGACCTTAGCATGAGCAACGAAATGTCTAGAAGCCCAA 
               
               
                   
                 GACCAAAAGTAGATTCCCTTTCA [T/C] GTGGGCCCTTCACCATTG 
               
               
                   
                 AATTGATGAACCTGTTGAGCAACGCGCTCAGTAACAATGACCCT 
               
               
                   
                 CCACTCAATCTTCCAGATTACAGGATGCAACTTCATCTTCCAGAT 
               
               
                   
                 GCCAGGATTACAACTTCAACACCGCTAGATCTGAGGTGTCACTC 
               
               
                   
                 TCCACAAATGAGCTTATCCTGACTATAAAGAGTAACCATATCAC 
               
               
                   
                 CACAG 
               
               
                   
               
               
                 BICFG630J749105 
                 TGTTGCATTAATGGCTTATTTTGCATATCTGTTTGATTGTACCAT 
               
               
                 (SEQ 1D NO: 214) 
                 CTTAAAGATTCATTTAAACCTGGGGAGGACAGCATGACTCCTAC 
               
               
                   
                 CTGCTTCTCATTAATAACATGCTTTTCAGTGAGTGGATAATGAAT 
               
               
                   
                 GACCTGGATAGAAATTAGCAGAAAATGCAGATTGCCATTAGGTG 
               
               
                   
                 TAGAAGTGGGGAGGGAGTCCAC [A/G] TTTTCCACCATACCGACA 
               
               
                   
                 AACAATTTCAAGTCAGAAGATATTGAAAACAAGCCTCAAAGAG 
               
               
                   
                 CCATAACTGTCTAGGAGGATTTTTAATTAAATATCCTGTTGTCTT 
               
               
                   
                 TACATGTAGAACTGTGAAGGAAAATGCATCCTAATAAAAATCAA 
               
               
                   
                 AATTTGCAAGTGACTTAAAAAATCTTGGGTTAATAGAAACAAGC 
               
               
                   
                 TATCTA 
               
               
                   
               
               
                 BICFG630J745699 
                 ACTCAAAGGAATGTACTGAGGTTTCTGAGGCATGAGAGCAAAA 
               
               
                 (SEQ ID NO: 215) 
                 GGGTCTAGGTGACAGACAACACTCAAAGTCTGATATGGGTTGTC 
               
               
                   
                 ATCCTGGTTCTCAGTTATTAGTCTTATAATAAGAACTCTGACCAT 
               
               
                   
                 ATCTGGAAATTCCATAACCCAGAACTCAACTTCCTGAGAAGGAA 
               
               
                   
                 CTTGTTAGATCTAGGCAGACAGAC [A/T] AGATAGTCTTCATTTGC 
               
               
                   
                 ACCAAGAAACTGAGGCAGAAGTTCAATCATCTAGACTGAATCAC 
               
               
                   
                 CATGGGTTAAGGGACAGAAAGGCCACAGGGACATAAGTCCAGG 
               
               
                   
                 GGTCACTCCAGGCTCACTGGACACCTTGCATGGGGAAGAATAAC 
               
               
                   
                 TAAGACCAAACCTATTAATTGGAAGAGATAAAGCTCCTAATACA 
               
               
                   
                 CTCCCAGC 
               
               
                   
               
               
                 Agouti A82S 
                 CGAGACAGACGTGAGGACAGGTGGGGTGGACGTGGCCGGCTTG 
               
               
                 (SEQ ID NO: 216) 
                 GGCAGCCCTGGCGTTTCCCTGCAGAAAAAGGCTTCGATGAAGAA 
               
               
                   
                 CGTG [G/T] CTCNTCCCCGGCCCCCGCCACCCACCCCCTGCGTGGC 
               
               
                   
                 CACTCGCAACAGCTGCAAGTCCCCGGCGCCCGCCTGCTGTGACC 
               
               
                   
                 CCTGCGCCTCCTGCCAGTGCCGCTTCTTCCGCAGCGCCTGCACCT 
               
               
                   
                 GCCGCGTTCTCAGTCCCAGATGCTGAGCGCGCCCAGCGGCCTCC 
               
               
                   
                 AGGGGGTTGGCTGAT 
               
               
                   
               
               
                 AGOUTI-R83H 
                 CGAGACAGACGTGAGGACAGGTGGGGTGGACGTGGCCGGCTTG 
               
               
                 (SEQ ID NO: 217) 
                 GGCAGCCCTGGCGTTTCCCTGCAGAAAAAGGCTTCGATGAAGAA 
               
               
                   
                 CGTGNCTC [G/A] TCCCCGGCCCCCGCCACCCACCCCCTGCGTGGC 
               
               
                   
                 CACTCGCAACAGCTGCAAGTCCCCGGCGCCCGCCTGCTGTGACC 
               
               
                   
                 CCTGCGCCTCCTGCCAGTGCCGCTTCTTCCGCAGCGCCTGCACCT 
               
               
                   
                 GCCGCGTTCTCAGTCCCAGATGCTGAGCGCGCCCAGCGGCCTCC 
               
               
                   
                 AGGGGGTTGGCTGATTATCTAAGAA 
               
               
                   
               
               
                 AGOUTI-R96C 
                 CGAGACAGACGTGAGGACAGGTGGGGTGGACGTGGCCGGCTTG 
               
               
                 (SEQ ID NO: 218) 
                 GGCAGCCCTGGCGTTTCCCTGCAGAAAAAGGCTTCGATGAAGAA 
               
               
                   
                 CGTGNCTCNTCCCCGGCCCCCGCCACCCACCCCCTGCGTGGCCA 
               
               
                   
                 CT [C/T] GCAACAGCTGCAAGTCCCCGGCGCCCGCCTGCTGTGACC 
               
               
                   
                 CCTGCGCCTCCTGCCAGTGCCGCTTCTTCCGCAGCGCCTGCACCT 
               
               
                   
                 GCCGCGTTCTCAGTCCCAGATGCTGAGCGCGCCCAGCGGCCTCC 
               
               
                   
                 AGGGGGTTGGCTGATTATCTAAGAA 
               
               
                   
               
               
                 MLPN-DILUTE 
                 GGAGGTAGATGAGCCTCTGGGGACGCCCCCCTCCTGCTGCCCAG 
               
               
                 COLOR 
                 GGCCGAGGGGCCCCCGGTCCTCTCTGTGAGGCTGACTCTGACTC 
               
               
                 (SEQ ID NO: 219) 
                 TCCTCCTCTTGCCCCTGCCTGCACCTGTGAAGAAAAAGC [G/A] CC 
               
               
                   
                 TCTCCTTCCACGACTTGGACTTTGAGGCAGACTCTGACGACTCCA 
               
               
                   
                 CTTGGTCTGGAAGTCACCCCCCCCACTCGTCCCCAGTCTCAGTGG 
               
               
                   
                 CCACAGACAGCCTGCAGGTCAGTGGGCTCATTTCTGGCCCCCCA 
               
               
                   
                 GCCTTCCCGGGATAACCTGAGCGACAGGTACGTGGGCCCCAGGT 
               
               
                   
                 GGGGGACGGGGCGCTCTGGGAAGGAGTCCGATGGCCATATCAA 
               
               
                   
                 GCTTCGGGG 
               
               
                   
               
               
                 MASK 
                 ATGGTCTGGCAGGGCCCCCAGAGAAGGCTGCTGGGCTCTCTCAA 
               
               
                 (SEQ ID NO: 220) 
                 TGGCACCTCCCCAGCCACCCCTCACTTCGAGCTGGCTGCCAACC 
               
               
                   
                 AGACCGGGCCCCGGTGCCTGGAGGTGTCCATTCCCAACGGGCTG 
               
               
                   
                 TTCCTCAGCCTGGGGCTGGTGAGCGTTGTGGAAAATGTGCTGGT 
               
               
                   
                 GGTGGCCGCCATTGCCAAGAACCGCAACCTGCACTCGCCCATGT 
               
               
                   
                 ATTACTTCATCGGTTGCCTGGCTGTGTCCGACCTGCTGGTGAGCG 
               
               
                   
                 TGACGAATGTGCTGGAGACGGCCGTCATGCTGCTGGTGGAGGCA 
               
               
                   
                 GGCGCCTTGGCTGCGCAGGCTGCTGTGGTGCAGCAGCTGGACGA 
               
               
                   
                 CATCATTGACGTGCTCATCTGTGGTTCCATGGTATCCAGCCTCTG 
               
               
                   
                 CTTCCTGGGCGCCATCGCCGTGGACCGCTACCTCTCCATCTTCTA 
               
               
                   
                 CGCGCTGCGATACCACAGCATCGTCACACTCCCGCGGGCGTGGC 
               
               
                   
                 GGGCCATCTCCGCTATCTGGGTGGCTAGCGTCCTCTCCAGCACG 
               
               
                   
                 CTCTTCATTGCCTACTACAATCACACGGCCGTCCTGCTTTGTCTT 
               
               
                   
                 GTCAGCTTCTTTGTAGCCATGCTGGTGCTCATGGCAGTGCTGTAC 
               
               
                   
                 GTCCACATGCTTGCCCGCGCCCGCCAGCACGCCCGAGGTATTGC 
               
               
                   
                 CCGGCTCCGTAAGCGGCAGCACTCCGTCCACCAGGGCTTTGGCC 
               
               
                   
                 TCAAGGGCGCTGCCACACTCACTATCCTGCTGGGCATTTTCTTTC 
               
               
                   
                 TCTGCTGGGGCCCCTTCTTCTTGCACCTCTCACTC [A/G] TGGTCCT 
               
               
                   
                 CTGCCCTCAACACCCCATCTGTGGCTGCGTCTTTCAGAACTTCAA 
               
               
                   
                 CCTCTTCCTCACCCTCATCATCTGCAACTCCATCATTGACCCCTT 
               
               
                   
                 CATCTACGCCTTCCGCAGCCAGGAGCTCCGAAAGACTCTCCAAG 
               
               
                   
                 AGGTAGTGCTATGTTCCTGGTGA 
               
               
                   
               
               
                 MC1R_YELLOW 
                 CATTTTCTTTCTCTGCTGGGGCCCCTTCTTCTTGCACCTCTCACTC 
               
               
                 (SEQ ID NO: 221) 
                 ATGGTCCTCTGCCCTCAACACCCCATCTGTGGCTGCGTCTTTCAG 
               
               
                   
                 AACTTCAACCTCTTCCTCACCCTCATCATCTGCAACTCCATCATT 
               
               
                   
                 GACCCCTTCATCTACGCCTTCCGCAGCCAGGAGCTC [C/T] GAAAG 
               
               
                   
                 ACTCTCCAAGAGNTAGTGCTATGTTCCTGGTGAGGCTGCAGGCT 
               
               
                   
                 TGAGGCCAGGGTGCTGGCCAGAGGGGGGTGGTGATTGATACCC 
               
               
                   
                 ATGTGACTGGGGCAGTCACTTGCAGAAAAGGACAGATGAGCTG 
               
               
                   
                 ATCTGTGGTGTGGTGGATGCATGGACCCTCTGGGGCCAGAGAAA 
               
               
                   
                 GGAATAAACAAAAATCTCCAGGAGTTGCTGTGGAGAATGGAGC 
               
               
                   
                 AGGCTGAGGAGATGGTGGGGCCACA 
               
               
                   
               
               
                 TYRP1_345P 
                 TACATATCCCATCCTTTTCCCAGGTACTGAGGGTGGGCCAATTA 
               
               
                 (SEQ ID NO: 222) 
                 GGAGAAATCCAGCTGGAAATGTGGCTAGACCAATGGTGCAACG 
               
               
                   
                 TCTTCCTGAACCANAGGATGTCGCTCAGTGNTTGGAAGTTGGTT 
               
               
                   
                 TATTTGACACA [CCT/*] CCTTTTTACTCCAATTCTACTAACAGTTT 
               
               
                   
                 CCGAAACACAGTGGAAGGTAAGTAAAAGAAATCAGTGCTTTGA 
               
               
                   
                 ATTCACAGTTAACTGAACTATTCACATTCAGATCTCTTTGAAAAA 
               
               
                   
                 TCTTTGAAAAACCATATAGATCCTGTGAATTTACATGAATGCTG 
               
               
                   
                 CCTCCAGTTATGATGTAGTCACAATTCTCTGCTCGAGAAAGAAC 
               
               
                   
                 TTCTTAAAGAAAAGTGTCAGACCGTGAAACTCTTTTTAATTATCA 
               
               
                   
                 TAGAGGAGAAGTGCTTAGAAATTAT 
               
               
                   
               
               
                 TYRP1-MC1R 
                 ATGAAAGCTCATAAACTCCTCTCTCTGGGAAGCATCTTCTTGTTC 
               
               
                 (SEQ ID NO: 223) 
                 CTGCTTTTTTTCCATCAGACCTGGGCTCAATTCCCAAGAGAGTGT 
               
               
                   
                 GCCACTGTTGAGGCCTTGAGAAATGGTGTG [T/C] GTTGCCCAGAC 
               
               
                   
                 CTGTCCCCAGTGTCTGGGCCTGGGACTGACCCCTGTGGCTNCTC 
               
               
                   
                 ATCAGGGCGGGGGAGGTGTGAGGCAGTGATAGCAGACTCCAGA 
               
               
                   
                 CCCCACAGCCACCATTACCCNCATGATGGCAGAGATGATCGGGA 
               
               
                   
                 GGTTTGGCCCACACGGTTCTTCAACAGGACCTGCCACTGCAATG 
               
               
                   
                 GCAATTTCTCAGGACACAACTGTGGGACTTGCCGTCCAGGATGG 
               
               
                   
                 AGAGGAGCTGCCTGTGATCAGAAGGTTCTCACAGTCAGGAGAA 
               
               
                   
                 ACCTCCTGGCCTTGAATACAGAAGAGAAGAACCACTTTGTCCAG 
               
               
                   
                 GCCTTGGATATGGCAAAGCGCACAATTCACCCTCAGTTTGTC 
               
               
                   
               
               
                 TYRP1_EX5 
                 TCTTGCTATGTGTAAAAATTAAAGGGCAAAGATCAGATCTCTAA 
               
               
                 (SEQ ID NO: 224) 
                 GTATCCTATAAATATTTACATATCCCATCCTTTTCCCAGGTACTG 
               
               
                   
                 AGGGTGGGCCAATTAGGAGAAATCCAGCTGGAAATGTGGCTAG 
               
               
                   
                 ACCAATGGTGCAACGTCTTCCTGAACCA [C/T] AGGATGTCGCTCA 
               
               
                   
                 GTGNTTGGAAGTTGGTTTATTTGACACANNNCCTTTTTACTCCAA 
               
               
                   
                 TTCTACTAACAGTTTCCGAAACACAGTGGAAGGTAAGTAAAAGA 
               
               
                   
                 AATCAGTGCTTTGAATTCACAGTTAACTGAACTATTCACATTCAG 
               
               
                   
                 ATCTCTTTGAAAAATCTTTGAAAAACCATATAGATCCTGTGAATT 
               
               
                   
                 TACATGA 
               
               
                   
               
               
                 Myotonia 
                 CCATTCCTCTCTGCCTCCCTTCCCTGCCCCTCCCATCTCTCTGTCT 
               
               
                 congenital 
                 CTCTCTCCCCTAGTAGCAGCCATACTATTACACTGACATGCTGA 
               
               
                 (SEQ ID NO: 225) 
                   [C/T] GGTGGGCTGTGCTGTAGGAGTTGGCTGTTGTTTTGGGACGC 
               
               
                   
                 CACTTGGAGGCAAGTGATTTACCCCTCCTACATCAGTCCGCTGCT 
               
               
                   
                 TGGGCTTGCTCCCCAGCCAGGTTTTGTCAGCATCCCCAAGTGTG 
               
               
                   
                 ACATTACCAGTTACAACAA 
               
               
                   
               
               
                 CLAD_1 
                 GCCGCGTGGGGTCGGCCCGCGTCAGGCCACCTCTCACGGAGCTG 
               
               
                 (SEQ ID NO: 226) 
                 CCTCCTCCTGCCGCCAGCGTCCTGCCAGGAGTGCACCAAGTACA 
               
               
                   
                 AAGTGAGCACGTGCCGGGACT [G/C] TGTGGAGTCGGGGCCCGGC 
               
               
                   
                 TGCGCCTGGTGCCAGAAGCTGGTAAGAGCCCCCCCCCAGGGACC 
               
               
                   
                 TCGCGCCCGTCCTGCCCGTCCCGCGTTCCCGTCCCCGTTCCTGTC 
               
               
                   
                 CCCACGCCCTCCCTCTGCCTCT 
               
               
                   
               
               
                 CLN2-TPP1 
                 GGAAAATACCTGACCCTAGAGGATGTGGCTGAACTGGTCCGGCC 
               
               
                 (SEQ ID NO: 227) 
                 ATCACCACTGACCTTCCGCACAGTCCAAAAATGGCTCTCAGCAG 
               
               
                   
                 CTGGAGCC [C/*] GGAACTGCCACTCGGTGACCACACAAGACTTTC 
               
               
                   
                 TGACTTGCTGGCTGAGTGTCCGA 
               
               
                   
               
               
                 CLN5_B_C 
                 CTAGGAAACACATTTAACCAAATGGCAAAGTGGGTAAAGCGGG 
               
               
                 (SEQ ID NO: 228) 
                 ACAATGAAACAGGAATTTATTACGAGACGTGGACTGTT [C/T] AA 
               
               
                   
                 GCCAGCCCAACAAAGGGGGCTGAGACATGGTTTGAATCCTATGA 
               
               
                   
                 TTGTTCTAAATTCGTGTTAAGGACATACAAGAAGTTGGCTGAAC 
               
               
                   
                 TTGGAGCAGAGTTCAAGAAGATAGAAACCAACTATACAAGAAT 
               
               
                   
                 ATTTCTTTACAGTGGAGAACCTACC 
               
               
                   
               
               
                 CLN8_DOG 
                 GAGAACGTAGCAGTTCACCTGTCCAATGTGCTCTTCCGGACATT 
               
               
                 (SEQ ID NO: 229) 
                 TGACTTGTTTTTGGCCATCCACCATCTCTTCGCCTTTCTGGGATTT 
               
               
                   
                 CTTGGCTCCGTGGTCAACCTCGGAGCCGGCCACTATCTGGCTAT 
               
               
                   
                 GAGCACGC [T/C] CGCTTCTGGAGGCGAGCACTCCCTTCACCTGCA 
               
               
                   
                 TTTCCTGGATGCTCCTAAAGG 
               
               
                   
               
               
                 TFT_CH 
                 ACGCCCACCCTCCAACCCGCACGGTGCTCGGTGCTGCTCCCCGG 
               
               
                 (SEQ ID NO: 230) 
                 CTCCTGCCCCCACAGTCCGCTCGAGAGCTTAGCGGTTGCTTCTGA 
               
               
                   
                 GTGACCCACTTTATGTCCCCCCTTGTGCCATAGAGACCTCAGCA 
               
               
                   
                 AAAGGGGGCTCCCTTCCCCGTCGCAGCTTCTGTCTTTTTCCAAAC 
               
               
                   
                 TCCCCGAGCCGACCAGCCGAGCCGTCTCCCGAGCGGCGGAGATC 
               
               
                   
                 ATGGAAGCCTCCGTGCAGGCCGTGAGGACACGGGTCTATGGGA 
               
               
                   
                 AGCTGGGG [C/T] GATCTTGGCCTCTCACCGGTAAGCGCCCCGAG 
               
               
                   
                 GGGCAGGCCCCAGGGCCTCGACCCAGGACAGCATGGCCAAGGG 
               
               
                   
                 AAGGTATCTGGGTTCCCCTGGGAGGTCCTGCAGCCCCCTCCGGA 
               
               
                   
                 CGTGGGGAAACGGCTCAAGCCCCCAGGCAGCCCCGTCCTGGCAT 
               
               
                   
                 CAGAGAGTGTGGGGGTGTTGGCGCGGCCCGGACGGAAGGTGGG 
               
               
                   
               
               
                 CYST_NEWFOUN 
                 AGGTATTCAAGAGAAACTGGACTACATCACAACTTTAAATATAA 
               
               
                 (SEQ ID NO: 231) 
                 AAACCATTTGGATTACTTCATTTTACAAATCATCCCTTAAAGATT 
               
               
                   
                 TCCGATATGGTATCGAAGACTTC [C/T] GAGACATTGATCCTATTT 
               
               
                   
                 TTGGAACAATGAAAGATTTTGAGAATCTGCTTGCAGCCATACAC 
               
               
                   
                 GATAAAGGT 
               
               
                   
               
               
                 Dach_narco 
                 GTCCGGCACCAAACTGGAGGACTCCCCCCCTTGTCGCAACTGGT 
               
               
                 (SEQ ID NO: 232) 
                 CATCTGCTCCGGAGCTGAATGAAACTCAAGAGCCCTTTTTAAAC 
               
               
                   
                 CCCACCGACTATGACGACGAGGAATTCCTGCGGTACCTGTGGAG 
               
               
                   
                 GGAATACCTACACCCGAAAGAATAT [G/A] AGTGGGTCCTGATCG 
               
               
                   
                 CTGGCTACATCATCGTGTTCGTGGTGGCTCTCGTGGGCAACGTCC 
               
               
                   
                 TGGGTGAGTCTGGCCCCGGGCAGCCCTCCCGAGGGCTGTCACGG 
               
               
                   
                 CCCCTCTGCGCGGGCGGGGCTGCCGGGGCTCTGAAGAC 
               
               
                   
               
               
                 DYSTROPHIN 
                 ATAAAGAGTAACACTCTTAAGGAATGATGGGCATGGGTTGTCAA 
               
               
                 (SEQ ID NO: 233) 
                 TTAAAAATCAGAAATGAAGTGAATCTTGTGAAATATTGTAAATT 
               
               
                   
                 GATTTATATTTATTTTTATGTGTGTGTGTTTCAG [GCCAG/*] ACCT 
               
               
                   
                 GTTTGATTGGAATAGTGTGGTTTGCCAGCAGTCAGCCACACAAC 
               
               
                   
                 GCCTGGAACATGCATTCAACATTGCCAAATATCAATTAGGCATA 
               
               
                   
                 GAGAAACTGCTTGATCCTGAAGGTCGGTACATTTCTGGACTACC 
               
               
                   
                 ATAGTTTTTAGTATAGTTTAATATTTATAATCTCAGA 
               
               
                   
               
               
                 globoid cell 
                 GGGTTGCCATGGTCATTTCCTGGATGGATAGGAAAAGGTTTCAA 
               
               
                 leucodystrophy 
                 CTGGCCTTACGTGAATCTTCAGCTGACTGCCTACT [A/C] TATCAT 
               
               
                 (SEQ ID NO: 234) 
                 GACCTGGATTGTGGGTGCCAAGCATTATCATGATTTGGACATTG 
               
               
                   
                 ATTATATCGGG 
               
               
                   
               
               
                 GM-gangliosidosis 
                 ATCGACTCTATCTCCTGTGGCTCTTGCTTGTCACCATTGCCTATA 
               
               
                 (SEQ ID NO: 235) 
                 ACTGGAACTGCTGGCTTATACCACTACGCCTCGTCTTTCCATATC 
               
               
                   
                 AAACACCAGACAACACACACTACTGGTTTATTACAGACATCACA 
               
               
                   
                 TGTGATATCATCTACCTTTGT [G/A] ATATGCTATTAATCCAGCCC 
               
               
                   
                 AGACTCCAGTTTATAAAAGGAGGAGACATAAT 
               
               
                   
               
               
                 GM-gangliosidosis 
                 ATGCTTCCCAGAGGACATTCACAATTGACTACAGCCACAACCGC 
               
               
                 (SEQ ID NO: 236) 
                 TTCCTGAAGGACGGCCAGCCCTTCCGCTACATTTCGGGAAGCAT 
               
               
                   
                 TCACTATTCCCC [G/A] TGCCCCGCTTCTACTGGAAGGACCGGCTG 
               
               
                   
                 CTGAAGATGAAGATGGCTGGGCTGAATGCCATCCAGACGTAAGT 
               
               
                   
                 AAGAGGGCGCTGGGCTCTCACCTGGGCCTAGACACCCATACCTG 
               
               
                   
                 GAGAGAGAGAGCAGCTGGATC 
               
               
                   
               
               
                 Hemophilia B 
                 GGTGCCTAAGGTGGCTGGCACTGACTTGCCGTACCCTCCCCATG 
               
               
                 (SEQ ID NO: 237) 
                 TCTCCTTGTGTCTGCAGTGGGTGAATGGGGTCCATGTGGCAGAG 
               
               
                   
                 CACGAGGGGGGTCACCTCCCCTTCGAAGCTGACATCAGCAAGTT 
               
               
                   
                 GGTCCAGAGCGGGCCCCTGTCCTCCTGCC [G/A] TATTACCCTTGC 
               
               
                   
                 CATCAACAACACGCTCACCCCCCACACTCTGCCGCCAGGGACCA 
               
               
                   
                 TCGTCTACAAGACAGACGCTTCCAAGTGAGCAGCACTCTGCTCC 
               
               
                   
                 CCTGCCCCCCCTGCCCCCCACCCACTGGGCTTCCGACT 
               
               
                   
               
               
                 hereditary cataracts 
                 CCGAGCCACGTGCCTTCGGTCCACGAAGTTCACCATTTATAACA 
               
               
                 (SEQ ID NO: 238) 
                 ACATGTTCTGTGCTGGCTTCCATGAGGGAGGTAAAGATTCATGC 
               
               
                   
                 CAGGGCGATAGTGGGGGACCCCATGTCACCGAAGTAGAAGGCA 
               
               
                   
                 TAAGTTTCTTAACTG [G/A] GATTATTAGCTGGGGTGAAGAGTGTG 
               
               
                   
                 CGATGAAAGGGAAGTATGGAATATATACCAAGGTGTCCCGGTAT 
               
               
                   
                 GTCAACTGGATTAAAGAAAAGACGAAGCTCACCTAAAGAATAA 
               
               
                   
                 TGTATTTCCAAGGTTGACACGTTTAGGGTAGAAAATGGACAAGG 
               
               
                   
                 TCCTTTACTAACTAATCACTTTTTTTATCTCTTTAGATTTGACTAT 
               
               
                   
                 ATACATTCTC 
               
               
                   
               
               
                 hereditary cataracts 
                 GTGCAGGGAGAAGGGCCTGGCACTGCTCAAAGAAGAGCCGGCC 
               
               
                 (SEQ ID NO: 239) 
                 AGCCCAGGGGGGGAAGGCGAGGCCGGGCTGGCCCTGGCCCCAA 
               
               
                   
                 ACGAGTGTGATTTTTGCGTGACAGCCCCCCCCCC [*/C] ACTGTCC 
               
               
                   
                 GTGGCTGTGGTGCAGGCCATCCTGGAAGGGAAGGGGAACTTCA 
               
               
                   
                 GCCCCGAGGGGCCCAGGAATGCCCAACAGCCTGAACCAAGGGG 
               
               
                   
                 TCCCAGGGAGGTACCTGACAGGTGAGC 
               
               
                   
               
               
                 PRA 
                 AGGAGTTTTCCCGTTTCCACGAAGAGATCCTGCCCATGTTCGAC 
               
               
                 (SEQ ID NO: 240) 
                 GACTGCAGAACAACAGGAAGGAAT [G/A] GAAGGCCTTGGCTGA 
               
               
                   
                 TGAGTACGAGGCCAAGCTGAAGGCCCTGGAGGAGGAGAAGCAG 
               
               
                   
                 CAACAAGAGGACAGGACGACAGCCAAGAAAG 
               
               
                   
               
               
                 PHOSPHOFRUCTOKINASE 
                 GTGTTCTGGGGATGCGTAAGAGGGCTCTGGTCTTTCAACCAGTG 
               
               
                 DEFICIENCY 
                 ACTGAGCTGAAGGACCAGACAGATTTTGAGTGAGTACATCTGCT 
               
               
                 (SEQ ID NO: 241) 
                 TCCCTGGTAGTTTCAGGGTCTGCTCTTCCCAGCCTGTGTGCTGCC 
               
               
                   
                 TTCAATCCTCTCATCCTAGGACTAACACCGTCATCACACCTATTT 
               
               
                   
                 CAGATCTTAACCCCGTGCCCTAAAATCCGGCCTCTTCTACTCAAC 
               
               
                   
                 TTCTTTCCATAAGCTTTGGATAGAAGTCAGTTGGGTTGCTAAAA 
               
               
                   
                 GCTGAAATCATCATCTCTCTCATTTCTCTGTAGTCACCGCATCCC 
               
               
                   
                 CAAGGAACAGT [G/A] GTGGCTGAAGCTGAGGCCCATCCTCAAAA 
               
               
                   
                 TCCTAGCCAAGTACGAGATTGACTTGGACACCACAGAGCACGCC 
               
               
                   
                 CACCTGGAGCACATCAGTCGGAAGCGATCTGGAGAAACTTCTAT 
               
               
                   
                 CTAACCCTCTTTGGAGTGAGGGTCATGGATTGTCTGATCATGGTC 
               
               
                   
                 AGCTCACCCCCTGATAGATCCAAGTCCATGTATCCCCAAGTATTT 
               
               
                   
                 TAGCTCATTTTTCTTTAGGTTTCCTTTTATTCTGCAACTGTAGCCA 
               
               
                   
                 TGACCAGCTCTGGCCAGGGAGCTGGGGCAGCGGGCAGTGAGTA 
               
               
                   
                 GAGGCTCCTTTTAGGTGGAATTTATCAACTTCTACCCCAGCTTCA 
               
               
                   
                 TCTGTCACACAAGACTGGGCTCCTCTAGTGCTACTGCTAGATTTC 
               
               
                   
                 AGCTACTCGGTTAGAATTTTCCTGAAAATAAGCTTTATTTATTTC 
               
               
                   
                 TTTGTGATAACAAAGTCTTGGTTCCTCTATTACTTTTACTGCAGT 
               
               
                   
                 GACAAACAATAGCTACACTAATAAATGCCAACTGGTCACTGTGC 
               
               
                   
                 TTTTGGTTCTCCTGTTGTCACTTTCACAAGTGAATGTCATCCTGT 
               
               
                   
                 CAACC 
               
               
                   
               
               
                 PRA 
                 CAGAGCCTGAAGTCGTCCTGCCGGAGCCCTGGGTGGCCAAGCTC 
               
               
                 (SEQ ID NO: 242) 
                 AGGCCTCAGCAGCACTCTTNGGACTGAGCCGCCCACGGGGCAGC 
               
               
                   
                 CGCCAGGACCGCAGCCATGAACGGGA [C/G] GGAGGGCCCGAAC 
               
               
                   
                 TTCTACGTGCCCTTCTCCAACAAGACGGGTGTGGTGCGCAGCCC 
               
               
                   
                 CTTCGAGTACCCACAGTACTACCTGGCTGAGCCATGGCAGTTCT 
               
               
                   
                 CCATGCTGGCTGCCTACATGTTTCTGCTGATCGTGCTCGGCTTCC 
               
               
                   
                 CCATCAACTTCCTCACGCTCTAC 
               
               
                   
               
               
                 Thrombasthenic 
                 GCGGCACGACTTGCTGGTGGGCGCGCCACTGTTCATGGAGAGCC 
               
               
                 thrombopathia 
                 GCGCGGACCGCAAGCTGGCCGAGGTGGGGCGCGTGTACTTGTTC 
               
               
                 (SEQ ID NO: 243) 
                 CTGCAGCCTCGAGGTCACCAGGCGCTGGGCGCCCCCAGCCTCCT 
               
               
                   
                 GCTGACTGGCACACAGCTCTATGGGCGATTCGGCTCGGCCATCG 
               
               
                   
                 CATCTCTGGGCGACCTC [G/C] ACCGGGACGGCTACAACGGTAAG 
               
               
                   
                 GGGCAGAGAGGAGCACCGCTTGCTTCAGACTGGTTAACAGCCA 
               
               
                   
                 GAACCAAGACCGCCGATTTGACCAGAGGGCAGCCAGAGCGGGG 
               
               
                   
                 AAGGGCTTTTCTCTGGAAGAGTTGAATGGGACCAGTTTGTTTGC 
               
               
                   
                 ATTGGTCCAGGC 
               
               
                   
               
               
                 SCID 
                 GATCTTTGGAAGATATTTGATTACCTAACCTTGGTAATTGTTTTA 
               
               
                 (SEQ ID NO: 244) 
                 TAGGATTAAAACTAAGTTGGATCTAGGAGGAGTGATTCAAGATT 
               
               
                   
                 TTATTAGTGCCCTAGAACAGCTCTCTAATCCTGAAATGCTCTTTA 
               
               
                   
                 AGGTAATGTAATAGCTTCTAACTCATAAAACATAGAATTTGGAT 
               
               
                   
                 TGAACTTACTTGCAGTCAACTTGGTTTTTCCCTCTCTCTCTCTTTT 
               
               
                   
                 TTTTTTTTTTTTTTGCACAGGATTGGACTGATGATATGAAAGCCG 
               
               
                   
                 AACTGGCAAAAAACCCTGTTAATAAAAAAAACATTGAAAAGAT 
               
               
                   
                 GTAT [G/T] AAAGAATGTATGCAGCTTTGGGAGATCTAAGGGCTC 
               
               
                   
                 CAGGGCTTGGGGCTTTCAGAAGGAGGTTTATTCAGGTAGGGATA 
               
               
                   
                 GGTGGCAGCCTGCCTATATAATAATGGAATCATTGTAACAATCA 
               
               
                   
                 GTAGTTATATTTTCTGGCTTGTTAATAATCCTGG 
               
               
                   
               
               
                 WELSH_SCID 
                 CCTTCAGGATCCTAACTTGTTCAGGCCAGGGGAATGACCACACA 
               
               
                 (SEQ ID NO: 245) 
                 CACACACATATCTCCAGTGATCCCCTGGGCTCCGGAGAACCTAA 
               
               
                   
                 CCCTTCACAACCTGAGCGAATCCCAGCTAGAACTGAGCTGGAGC 
               
               
                   
                 AACAGACACTTGGACCACTGTTTGGAGCATGTTGTGCAGTACCG 
               
               
                   
                 GAGTGACTGGGACC 
               
               
                   
                   [*/C] GCAGCTGGACTGTGAGTGACTTGGGTCATGAAGGTGGCAG 
               
               
                   
                 CAAAGGCCAAGCAAATAGGGATAAAGGATTCAATCAGC 
               
               
                   
               
               
                 SCID_X 
                 GTTTCTAAGGTTCTTTCCACCGGAAACTATGACAGAAGGAAATG 
               
               
                 (SEQ ID NO: 246) 
                 TGTGGGTGGGGAGGGGTAATGGGTGAGGGGCCCAGGTTCCTGA 
               
               
                   
                 CAGTCTACACCCAGGGAACGAAGAGCAAGCGCCATGTTGAAGC 
               
               
                   
                 CACCATTGCCACTCAGATC [CCTC/*] TTATTCCTGCAGCTGTCTCT 
               
               
                   
                 GCTGGGGGTGGGGCTGAACTCCACGGTCCCCATGCCCAATGGGA 
               
               
                   
                 ATGAAGACATCACACCTGGTGGGAAACATGGGACTGGAAGGGG 
               
               
                   
                 TTGGTGAGAGGGGAGCCTGTGGGAAGGGGTCGCATAGAAATCT 
               
               
                   
                 TGAACCTGCCATGGGGCATTAGAAGGATGTGGGCAGAGTTTAAG 
               
               
                   
                 AGTGCTGTGGAGA 
               
               
                   
               
               
                 SCN_DOG 
                 GGAAGGCTAAGTGGAGCAAATAAATGTTTGTTCTGAAACATTAA 
               
               
                 (SEQ ID NO: 247) 
                 GAATTACTTCATTGACTTTTTAACAGAATATGCAATAAATTAAAT 
               
               
                   
                 ATTTCTTATCTATAGGAGAAAGAAAAAAAAA [*/A] CAAAGGAAG 
               
               
                   
                 ATAGAAATCTTACCAAAGATGTTTCACTTCTAGACCTGGATGAT 
               
               
                   
                 TGTAAGTGTTGAAATTTAAATTTTTTCTTCTCTTTTTAGTAGTAG 
               
               
                   
               
               
                 retinal dystrophy 
                 TTAGCCCTTTTCTTTCACAGCTTGAAGGTTACTGGACTGAAAAAC 
               
               
                 (SEQ ID NO: 248) 
                 TCCGTTTGCTTCTGTAGGTTTTTTTCTTACTTCCGAGGAGTGGAG 
               
               
                   
                 GTCACTGACAATGCCCTTGTTAACGTCTACCCAGTAGGGGAAGA 
               
               
                   
                 TTACTANGCCTGCACGGAGACCAACTTCATTACA [AAGA/*] TTAA 
               
               
                   
                 TCCTGAGACCCTGGAGACAATTAAGCAGGTAGGACGAAATGCTC 
               
               
                   
                 AGGCGACGTTGCTCAAGAATTTAGAATTTGCAGTTTAGATTTAA 
               
               
                   
                 CTGCAATTTTGGGGAAAGCTCATGAGGGCCAAATAGATTGTCTC 
               
               
                   
                 GCTGCCTTGCTTTGTCATCAACTACTAGCCATGTGACACGAGGC 
               
               
                   
                 ACTCTTTA 
               
               
                   
               
               
                 type-2 von 
                 GGGATATCCGATACCGGGGTGGCAACAGGACCAACACTGGACT 
               
               
                 Willerbrand&#39;s 
                 GGCCCTGCAATACCTGTCCGAACACAGCTTCTCGGTCAGCCAGG 
               
               
                 (SEQ ID NO: 249) 
                 GGGACCGGGAGCAGGTACCTAACCTGGTCTACATGGTCACAGG 
               
               
                   
                 AAACCCCGCTTCTGATGAGATCAAGCGGATGCCTGGAGACATCC 
               
               
                   
                 AGGTGGTGCCCATCGGGGTGGGTCCACATGCCAATGTGCAGGAG 
               
               
                   
                 CTGGAGAAGATTGGCTGGCCCA [A/G] TGCCCCCATCCTCATCCAT 
               
               
                   
                 GACTTTGAGATGCTCCCTCGAGAGGCTCCTGATCTGGTGCTACA 
               
               
                   
                 GAGGTGCTGCTCTGGAGAGGGGCTGCAGATCCCCACCCTCTCCC 
               
               
                   
                 CCACCCCAG 
               
               
                   
               
               
                 Type III von 
                 TGTCGCTCCCTCTCTTACCCGGAGGAGGACTGCAATGAGGTCTG 
               
               
                 Willebrand 
                 CTTGGAAGGCTGCTTCTGCCCCCCAGGGCTGTACCTGGATGAGA 
               
               
                 (SEQ ID NO: 250) 
                 GGGGAGATTGTGTGCCCAAGGCTCAGTGTCCCTGTTACTATGAT 
               
               
                   
                 GGTGAGATCTTTCAGCCCGAAGACATCTTCTCAGACCATCACAC 
               
               
                   
                 CATGTG [G/A] TAAGTGCGAGCAGCATGACCAGGGACCTCAGGAA 
               
               
                   
                 TGGCGGAGCTTGTAAGGAAAATGGTCTTCTGGGTCCTTCATTTC 
               
               
                   
                 ACGGTTGGGAAACTGAGGCCCAGGAAGGGAAGTGACTTGCCCT 
               
               
                   
                 GAGTTGCACAGCTCGAATGATTTCCTTACATCGCTGGAAACTAG 
               
               
                   
                 AGCAGACTGCCA 
               
               
                   
               
               
                 Type III von 
                 GAAGGGAAAAATGAGTGAGTAAATTATATTTTGGGGAAGATTTT 
               
               
                 Willebrand 
                 TTTGTTGTTGTTCATTTGTTACGTCCTTGGGGAGAGTTCTCCATG 
               
               
                 (SEQ ID NO: 252) 
                 AGATGGGATTAATGATGTACATCAGATGATTAGAGGTAAATATC 
               
               
                   
                 CCGGCTTTTTTGGTAATAATCATAGTTACTGACTCTTTTCTCTTTC 
               
               
                   
                 AGGGGGTTTCCAAAATGGCAAAAGAGTGAGCCTCTC [C/*] GTGT 
               
               
                   
                 ATCTCGGAGAATTTTTCGACATTCATTTGTTTGTCAATGGTACCA 
               
               
                   
                 TGCTGCAGGGGACCCAAAGGTAAGTC 
               
               
                   
               
             
          
         
       
     
         [0081]    The present invention is not limited to species such as horses and dogs, but can be used in a variety of species. For example, the following tables demonstrate sequences that may be used determined genetic characteristics, such as parentage, identity, sex, genotype and/or phenotype and breed determination in cats. Thus, in further embodiments, the present invention provides a panel comprising a plurality of assay compositions, wherein each assay composition is capable of identifying at least one of the nucleotide markers as set forth in Tables 7 and 8 provided below: 
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 CAT SNP PANEL SEQUENCES 
               
             
          
           
               
                   
                 Cat Genomic 
                   
                   
                   
               
               
                 SEQ ID NO 
                 Location 
                 SNP CONTIG 
                 SNP 
                 Description 
               
               
                   
               
               
                 253 
                 Un: 51,831,052 
                 c200902194.Contig1 41887716 
                 A/G 
                 Many 
               
               
                 254 
                 c2: 703,930 
                 c201102843.Contig1 52683485 
                 A/G 
                 Many 
               
               
                   
                   
                 42085143 
               
               
                 255 
                 c2: 703,930 
                 c201102843.Contig1 52683485 
                 A/G 
                 Many 
               
               
                   
                   
                 42085143 
               
               
                 256 
                 E2: 64,720,639 
                 c209402154.Contig1 40390026 
                 A/G 
                 Many 
               
               
                 257 
                 D4: 812,589 
                 c210302384.Contig1 51478757 
                 C/T 
                 Many 
               
               
                   
                   
                 46990850 
               
               
                 258 
                 B4: 147,961,464 
                 c214001733.Contig1b00 
                 A/G 
                 Many 
               
               
                   
                   
                 40834831 41883837 
               
               
                 259 
                 B1: 156,143,186 
                 c216702119.Contig1 50170968 
                 A/G 
                 Many 
               
               
                 260 
                 F2: 77,518,182 
                 c217102268.Contig1 51882103 
                 T/G 
                 Many 
               
               
                 261 
                 A2: 17,611,273 
                 c218902205.Contig1 43673924 
                 C/T 
                 Many 
               
               
                 262 
                 B3: 107,303,663 
                 c220002309.Contig1 41798812 
                 A/C 
                 Many 
               
               
                 263 
                 D2: 74,626,676 
                 c221302563.Contig1 39163914 
                 C/T 
                 Many 
               
               
                 264 
                 A3: 88,919,777 
                 c221802646.Contig1 38897465 
                 C/T 
                 Many 
               
               
                 265 
                 A1: 151,473,414 
                 c222902793.Contig1 42602082 
                 C/G 
                 Many 
               
               
                 266 
                 B1: 178,757,633 
                 c223102384.Contig1 51610716 
                 C/T 
                 Many 
               
               
                 267 
                 B4: 19,612,127 
                 c225702363.Contig1 44291991 
                 A/C 
                 Many 
               
               
                 268 
                 E2: 11,112,283 
                 c226102304.Contig1 45346791 
                 C/T 
                 Many 
               
               
                 269 
                 A1: 15,263,737 
                 c228202754.Contig1 41061200 
                 C/T 
                 Many 
               
               
                 270 
                 A3: 40,227,427 
                 c229902453.Contig1 51587423 
                 A/G 
                 Many 
               
               
                 271 
                 C2: 150,072,397 
                 c230302478.Contig1 47807293 
                 A/G 
                 Many 
               
               
                 272 
                 B2: 43,290,061 
                 c231602346.Contig1 42950909 
                 A/G 
                 Many 
               
               
                 273 
                 D1: 124,939,879 
                 c232702561.Contig1 50699305 
                 A/G 
                 Many 
               
               
                   
                   
                 43049735 
               
               
                 274 
                 C1: 123,746,252 
                 c233302605.Contig1 45945358 
                 C/T 
                 Many 
               
               
                 275 
                 F2: 75,210,562 
                 c237202594.Contig1 39922895 
                 C/T 
                 Many 
               
               
                 276 
                 A3: 14,410,638 
                 c238102323.Contig1 51345702 
                 A/G 
                 Many 
               
               
                 277 
                 F1: 33,007,663 
                 c238602943.Contig1 43762371 
                 A/G 
                 Many 
               
               
                   
                   
                 42805370 45085530 
               
               
                 278 
                 A1: 208,380,043 
                 c239502892.Contig1 39703120 
                 A/G 
                 Many 
               
               
                   
                   
                 39628806 
               
               
                 279 
                 E2: 35,480,527 
                 c379002760.Contig1 38992791 
                 C/G 
                 Many 
               
               
                 280 
                 A3: 118,999,155 
                 c246003822.Contig1 42533812 
                 C/G 
                 Many 
               
               
                 281 
                 A2: 10,913,767 
                 c248603449.Contig1 43067711 
                 A/G 
                 Many 
               
               
                 282 
                 B2: 47,659,161 
                 c248803703.Contig1 41077986 
                 A/C 
                 Many 
               
               
                 283 
                 D2: 89,706,040 
                 c249103480.Contig1 51530567 
                 C/T 
                 Many 
               
               
                 284 
                 Un12: 7,317,515 
                 c252004127.Contig1 39641583 
                 C/T 
                 Many 
               
               
                 285 
                 Un2: 523,114 
                 c253404131.Contig1 37960459 
                 A/G 
                 Many 
               
               
                 286 
                 B2: 156,308,475 
                 c256404084.Contig1 54345379 
                 A/G 
                 Many 
               
               
                   
                   
                 43778944 
               
               
                 287 
                 A2: 25,685,296 
                 c259703305.Contig1 41812011 
                 C/T 
                 Many 
               
               
                 288 
                 A2: 2,129,037 
                 c261103489.Contig1 51387364 
                 C/T 
                 Many 
               
               
                 289 
                 A2: 161,801,210 
                 c263503219.Contig1 39442596 
                 A/C 
                 Many 
               
               
                 290 
                 D3: 7,290,581 
                 c265103456.Contig1 43475101 
                 A/G 
                 Many 
               
               
                 291 
                 F1: 19,516,618 
                 c267903188.Contig1 39678411 
                 A/G 
                 Many 
               
               
                 292 
                 D2: 82,189,281 
                 c278503306.Contig1 42981906 
                 C/T 
                 Many 
               
               
                   
                   
                 51016912 
               
               
                 293 
                 D1: 36,295,835 
                 c281903151.Contig1 52096151 
                 A/G 
                 Many 
               
               
                 294 
                 D3: 33,258,191 
                 c288803295.Contig1 44646770 
                 A/G 
                 Many 
               
               
                 295 
                 C2: 63,676,887 
                 c293703365.Contig1 40266370 
                 C/T 
                 Many 
               
               
                 296 
                 A3: 48,181,817 
                 c372702909.Contig1 52632612 
                 C/T 
                 Many 
               
               
                 297 
                 A3: 11,904,341 
                 c297603245.Contig1 44279919 
                 A/G 
                 Many 
               
               
                 298 
                 E3: 63,458,569 
                 c298202957.Contig1 40800988 
                 A/C 
                 Many 
               
               
                 299 
                 B2: 112,716,268 
                 c302302970.Contig1 40606850 
                 A/T 
                 Many 
               
               
                 300 
                 B3: 149,673,110 
                 c307303163.Contig1 40903035 
                 C/T 
                 Many 
               
               
                 301 
                 C1: 125,311,520 
                 c314603195.Contig1 42747048 
                 A/G 
                 Many 
               
               
                 302 
                 B1: 19,312,704 
                 c315703075.Contig1 38246147 
                 C/T 
                 Many 
               
               
                 303 
                 B2: 120,276,458 
                 c315703352.Contig1 53559241 
                 C/G 
                 Many 
               
               
                 304 
                 B2: 159,389,942 
                 c332003111.Contig1 52844210 
                 A/G 
                 Many 
               
               
                 305 
                 D4: 39,362,745 
                 c337003053.Contig1 41695419 
                 C/T 
                 Many 
               
               
                 306 
                 B1: 172,534,764 
                 c354102993.Contig1 39140016 
                 A/G 
                 Many 
               
               
                 307 
                 POINTED1 
                   
                 
                   G/A 
                 
                 Many 
               
               
                 308 
                 POINTED2 
                   
                 
                   G/T 
                 
                 Many 
               
               
                 309 
                 ALBINO 
                   
                 
                   C/* 
                 
                 Many 
               
               
                 310 
                 CHOCOLATE 
                   
                 
                   G/A 
                 
                 Many 
               
               
                 311 
                 CINNAMON 
                   
                 
                   C/T 
                 
                 Many 
               
               
                 312 
                 Mucopolysaccharidosis 
                   
                 
                   T/C 
                 
                 Many 
               
               
                   
                 Type VI 
               
               
                 313 
                 Mucopolysaccharidosis 
                   
                 
                   G/A 
                 
                 Many 
               
               
                   
                 Type VI MILD 
               
               
                 314 
                 Polycystic Kidney 
                   
                 
                   C/A 
                 
                 MANY 
               
               
                   
                 Disease 
               
               
                 315 
                 Hypertrophic 
                   
                 
                   G/C 
                 
                 MAIN COON 
               
               
                   
                 cardiomyopathy 
               
               
                   
                 MC 
               
               
                 316 
                 Hypertrophic 
                   
                 
                   C/T 
                 
                 RAGDOLL 
               
               
                   
                 cardiomyopathy 
               
               
                   
                 RG 
               
               
                   
               
             
          
         
       
     
         [0082]    The nucleic acid sequences of the markers of Table 7 are provided in Table 8 below, where some polymorphic sites (e.g., the single nucleotide polymorphism (SNP), insertion and/or deletion) are bracketed and indicated in bold; however, those skilled in the art can readily identify other polymorphic sites by researching the particular sequence in corresponding cat registries or databases. Many sites may be identified. 
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 CAT SNP PANEL NUCLEOTIDE MARKER SEQUENCES 
               
             
          
           
               
                   
                 Cat Genomic Location 
                   
                   
               
               
                 SEQ ID NO 
                 and/or Description 
                 Sequence 
               
               
                   
               
               
                 253 
                 Un: 51,831,052 
                 TAGTCAGTCTTGGATACATTCGGCCACAGAGTCCTTC 
                   
               
               
                   
                   
                 AAAAATTGCCTTTCAGTCCTATGTTGACAAAGGTAAGT 
               
               
                   
                   
                 CCAGGGCATTTCAAGGTGCCCAACARGAGTGCTAAT 
               
               
                   
                   
                 GTGTAGTCAGGGTCAGAGATATTGGGAGGGAGCTAT 
               
               
                   
                   
                 CCTCACTTATGGGACAAGAGGAACATGGAGTTACACA 
               
               
                   
                   
                 CATAGGATAAATGAAAA 
               
               
                   
               
               
                 254 
                 c2: 703,930 
                 GGATGTGGAGAGATCGGAAGCCGTCCGCCCCGGTG 
               
               
                   
                   
                 GTGGGATTGCCAAACGGTATAGCTGTTCTAGAACACA 
               
               
                   
                   
                 GCCCGCGGTCCCCGGAAAAGTTACTATARGACTGTTA 
               
               
                   
                   
                 CGTGTCCCCGCAAGCCCACCTCCGACGCCCGACAGG 
               
               
                   
                   
                 ACTGACAGCAGGGTCCCGGAGAGAGGCACCTACGTC 
               
               
                   
                   
                 CGCAAAGGTAAGTGCGGGAG 
               
               
                   
               
               
                 255 
                 c2: 703,930 
                 GGATGTGGAGAGATCGGAAGCCGTCCGCCCCGGTG 
               
               
                   
                   
                 GTGGGATTGCCAAACGGTATAGCTGTTCTAGAACACA 
               
               
                   
                   
                 GCCCGCGGTCCCCGGAAAAGTTACTATARGACTGTTA 
               
               
                   
                   
                 CGTGTCCCCGCAAGCCCACCTCCGACGCCCGACAGG 
               
               
                   
                   
                 ACTGACAGCAGGGTCCCGGAGAGAGGCACCTACGTC 
               
               
                   
                   
                 CGCAAAGGTAAGTGCGGGAG 
               
               
                   
               
               
                 256 
                 E2: 64,720,639 
                 TAACACCTCTGAGCTGCATTTCCCTTCATTTGGGGCT 
               
               
                   
                   
                 GAATGACGAGAGGTGCAGAATGTTCTTTCCAAGGTTT 
               
               
                   
                   
                 TGGAGAGAATTCAGTGAGACAGTGGCRAACGGTGCC 
               
               
                   
                   
                 CGATACAGTAAGTGCTCAATAAAATACTAAAGCGGAA 
               
               
                   
                   
                 TCTAGTGGAAACTGCTCAACACCACCAGCGGTTTGGG 
               
               
                   
                   
                 GAGCTAAGAAGGCAACA 
               
               
                   
               
               
                 257 
                 D4: 812,589 
                 AAGTTCCCAGGATAGCTGCACACCAGGTACAGCGAG 
               
               
                   
                   
                 AAGACTGGGTCAGATCAAGAGGCTCTGGGGAGACAG 
               
               
                   
                   
                 TCTTCAGGGGCAGACAAGGATATACTGTCYGATGCAT 
               
               
                   
                   
                 CTGAACCAATCAGACATGGTGACAGGCTTCTTCACCT 
               
               
                   
                   
                 GATAAGAAGATTCAACTGGCAAGAAGCACACAGACAA 
               
               
                   
                   
                 CCAAGTTAGCAAAGCAGA 
               
               
                   
               
               
                 258 
                 B4: 147,961,464 
                 AGGGAGGAAATAAAGATGTTTGATTTATTACTGATAAC 
               
               
                   
                   
                 CCCGAGGTTTGAGTGTGCACCCAAAGGGATGTGCTG 
               
               
                   
                   
                 TGAATCTCCGCTTCTGAATGAGACACRCTCAACAGCC 
               
               
                   
                   
                 AGGACACTGGTACAGCTGGCAAACCACAAGCTACCC 
               
               
                   
                   
                 CTGTAGGAACAGGCGCCTTGCTGCATGGCGGAAAGC 
               
               
                   
                   
                 TAACCGGAAACCCCCACT 
               
               
                   
               
               
                 259 
                 B1: 156,143,186 
                 GTGTAGAGTGAGCTTGAGTACTTTGGCTTTTGTGTTTT 
               
               
                   
                   
                 GTACTCAAACCCACGGTCCTCTGGTTTCAAATCTGTG 
               
               
                   
                   
                 GTAGAGAACTACTCTTCCTTAGGTCRTTCTTGGATTCA 
               
               
                   
                   
                 CGCCAACCCTTCTCTCTAGTCCTTACCCATTGTCTTGT 
               
               
                   
                   
                 TCTCCCTTGATGATATCAGGAATCTTCTCCTATCTCAG 
               
               
                   
                   
                 GGTCTGAGTGTT 
               
               
                   
               
               
                 260 
                 F2: 77,518,182 
                 AAGCCTATGATAGAAACGAAGAGCCCATTCCAACCTA 
               
               
                   
                   
                 ACAGGTACCCTCAAATCACCCTGGTGCAAGGGCAAAA 
               
               
                   
                   
                 ACTCGGGAAGCAGGTCAGTCGTGGTTKGAAACCCATT 
               
               
                   
                   
                 TCTGTTGCTTTCTAGCAATTGTTTTTTGAAACTTTCTG 
               
               
                   
                   
                 CACCTCGGTTGCCTTACAGCGCTGAAGTGAGGGTCA 
               
               
                   
                   
                 CACGATTGAGGGTCTA 
               
               
                   
               
               
                 261 
                 A2: 17,611,273 
                 GTGAGCAAAAGTGGGTCAGGGTACAGACAGGCAGGG 
               
               
                   
                   
                 GGGTTCCAAGCAAAGCAAAACCCACATGAAGGCTCA 
               
               
                   
                   
                 GGGCTGTGCCTACCCCATCTGCACAATGYTAAAAATC 
               
               
                   
                   
                 TCACCTAATGTATTTAAACATCCCTTTTGTCTAGACCA 
               
               
                   
                   
                 TTCTCATATAGGTGTCAGGACGACCCCTAAAACAATC 
               
               
                   
                   
                 AGAATGTATCACTATAT 
               
               
                   
               
               
                 262 
                 B3: 107,303,663 
                 CTTGGAAAAATAGAATTTTACAGTTGGAAGGACTGTA 
               
               
                   
                   
                 CAGTCTTCCAAATCACTCACTTTCATTACTTGTTCCAC 
               
               
                   
                   
                 TGACAGCTTTCACTAAAAGATTACTMTGGAAAAACAG 
               
               
                   
                   
                 CTTCCCTTCTCATCCCTGAAGTGAGTCAAATTGCCTTT 
               
               
                   
                   
                 TCATGTATCTGTTCCAGAGGGCCATCTGCTCTGCCCC 
               
               
                   
                   
                 CAGGAGAAGCTTCT 
               
               
                   
               
               
                 263 
                 D2: 74,626,676 
                 GAATTTCTGCCCTTGTGTTCTGTGGCATCCCTCTCCTT 
               
               
                   
                   
                 GAATTTCTGACTTTTCACTTCTTTTATATCATTTAATTC 
               
               
                   
                   
                 TCATAACAGTCCTGGGAGGTAGGYGAGCAGAGATTAT 
               
               
                   
                   
                 TAACGTTTTTGAAGATGAGCACACTGAGGCCACATTA 
               
               
                   
                   
                 TTTAGCTTCTCTAAACGTTTCTCATCTGTTAAACGAGG 
               
               
                   
                   
                 AGCAGGACAAGA 
               
               
                   
               
               
                 264 
                 A3: 88,919,777 
                 GGTCCCAGTCTTACTTACTGTAAACCAGGAATAGCAC 
               
               
                   
                   
                 TACCTGCACCTTTGCATTGCATTGCAAACAACAATGA 
               
               
                   
                   
                 CTGACATTAAGAAAATCCTCAGTAAAYGTTGGCATTTT 
               
               
                   
                   
                 TTGTTAAATTCTTGACCCTATCATTTACTAGCTAAGGG 
               
               
                   
                   
                 AAGTCCGTGTAAGAGACTTCATTCTCTTCACACCACA 
               
               
                   
                   
                 GTTCTCCTCTATGC 
               
               
                   
               
               
                 265 
                 A1: 151,473,414 
                 AAAAGTGGAAATGTGTATTACAGAGGCAGTCCCAGGC 
               
               
                   
                   
                 ATGGCAGGCTCTGACAGGGTGTTGGAGATGATGGGT 
               
               
                   
                   
                 GGGCTTGAATACCCTGCCTGTGGGGGASGGGGGTGC 
               
               
                   
                   
                 TGGGAGAAGCACAAGGACCCAGGGGAGATGGCACC 
               
               
                   
                   
                 CTGCATGTCTGGGCCTGGGTGGGGGATTTAAGTGGT 
               
               
                   
                   
                 CCACGTCCCTATCTAGGAAGC 
               
               
                   
               
               
                 266 
                 B1: 178,757,633 
                 TTATCACTGGCCTCTTACTGTGGCCAGCCCCCACGAG 
               
               
                   
                   
                 AACTCCAGTGAGACAGCAGGCAATAGCTCATGAAGTG 
               
               
                   
                   
                 AAATCATTCAATGCCAAAAGGACTTCYGGGCCCCCGC 
               
               
                   
                   
                 CATGTGCAGGGAGCCACTTACACCCCAGCCACACGG 
               
               
                   
                   
                 AGGGAAGCAGGAGTGCTACACCGGTGGCAGAGAAGA 
               
               
                   
                   
                 GCCACCCCCCCAGGCCGT 
               
               
                   
               
               
                 267 
                 B4: 19,612,127 
                 TCCCTTGAGGGCACCCGCCTTAGATCACACCTTCTCT 
               
               
                   
                   
                 CAAGGTGCACGTGACAGGGCAAATCTTTTGCTTCCAG 
               
               
                   
                   
                 CCCAAGCTTGGTAGCTTAAGTGAATGMATTTAGTTTTA 
               
               
                   
                   
                 TGTAGATTCTGGTCTCCTGACCAGAAATCACTAGGAA 
               
               
                   
                   
                 GGAACAGGTTTGTCTAACATAGCTTGTAAGTGCCGGG 
               
               
                   
                   
                 TCCCTGCTGGCCATT 
               
               
                   
               
               
                 268 
                 E2: 11,112,283 
                 GGTCACAGACCCCAGGCCACCACCCACCTGGATGCC 
               
               
                   
                   
                 GGAAGGCTGGGCACTCCTGGAATGGCCGGGTCCAG 
               
               
                   
                   
                 CCTGGTTATTCCCTCGCCCGCAGCCAAGTYCATCCCT 
               
               
                   
                   
                 CCCGGTGGGGTCCACACCATCTTTCTTCCAAACCCCA 
               
               
                   
                   
                 CAGGTGCAAAGGGCCTCTTAGCAGCAACTACTTCCG 
               
               
                   
                   
                 GGGAGGGAGCAGGTGACAGC 
               
               
                   
               
               
                 269 
                 A1: 15,263,737 
                 TGATTCTTCAACAAACTCACAATCCACTTTAGTAATGG 
               
               
                   
                   
                 AAGCAGCTCTACCTTTGGCAAACAAAAGCAGAAAAAG 
               
               
                   
                   
                 TACAACCATGGCTGTGTAGAAGTCCYATATATCACTG 
               
               
                   
                   
                 CATGCTTAAACTTTACTCAGCAAAACTTTTAATTCTTTG 
               
               
                   
                   
                 GGGAAGGCAAGAGAGAAATAGTACCTGAAAACCAAG 
               
               
                   
                   
                 TATTAGTATTCTCA 
               
               
                   
               
               
                 270 
                 A3: 40,227,427 
                 TGGACTCTAGTTCTACCAATGGTTACTCTGAGATAATG 
               
               
                   
                   
                 CTTATCCTATCTTATGCTGGGAGGAAGCTGGTCAAGT 
               
               
                   
                   
                 AGGTATGAGCCTGTACAACTGCTGCRGGATCAGAGC 
               
               
                   
                   
                 TGCCTCAGGCCTCTGGTTTTAGAGGCCTCGTGATTTC 
               
               
                   
                   
                 CAAGGGAGGAAAAGGCCAGCATTGCTTGTCCTGTCA 
               
               
                   
                   
                 GCCTCCCTCCTGGTTTC 
               
               
                   
               
               
                 271 
                 C2: 150,072,397 
                 CATCCAGAAGTATTTAAAAACAATCTTTTTGCATGTCA 
               
               
                   
                   
                 TGTTTATTTATTTTTGAGAGAGACAGAGCACAAGCAG 
               
               
                   
                   
                 GGGAGGTGCAGAGAGAGACGGGGAGRCACAGGATT 
               
               
                   
                   
                 CGAAGCAGGCTCCAGGCTCCGAGCTGTCAGCACAGA 
               
               
                   
                   
                 GCCTGATGTGGGGCTGGAACTCAGACTGTGAGATCA 
               
               
                   
                   
                 TGACCTGAGCCAAAGTCGA 
               
               
                   
               
               
                 272 
                 B2: 43,290,061 
                 CCCAGGCATGAGGAGGGCAAGAGGGTAGGGCTGTA 
               
               
                   
                   
                 GTTGTCAGTGGGGCGAGCCCTGTCCCCCTGAGCCCT 
               
               
                   
                   
                 TGGTGGGGCTCTGACTCCCTAAAACTTTARAGGGAAG 
               
               
                   
                   
                 ATAACCTACCCTCAAATAGTGAGTGTTTTTCGCCCTTC 
               
               
                   
                   
                 CTCCTCAACTCTGAAACATTTGCAGTCAAGGGTAGTA 
               
               
                   
                   
                 GGGGACCCTAACCACAGG 
               
               
                   
               
               
                 273 
                 D1: 124,939,879 
                 ATCGGGCTCCACGCTGACAACGTGGAGCCTGCTTGG 
               
               
                   
                   
                 GATTCTCTGTCTCCCTCTCTCTGCCTCTCTTTCTCTCT 
               
               
                   
                   
                 CAAAAATAAATAAATAAACATTAAAGRAAGAAAAGAAA 
               
               
                   
                   
                 AAGAAATGGATTTGAGGAAGTATATCAAGCAAACAAA 
               
               
                   
                   
                 AACACGGGTTGGCGCAGGAGTAACAAAGTGGCAAAG 
               
               
                   
                   
                 TGTCGCCTAAATAGCA 
               
               
                   
               
               
                 274 
                 C1: 123,746,252 
                 GCTAAACATTCTACAACGTACACAACAAGGAGTAGTC 
               
               
                   
                   
                 ATTCCTGGTCCAAAATATTGATTGTGTTGAGCCTGAG 
               
               
                   
                   
                 AAACTCCTATTTAAATAACTGAGTTCYCTTTTCATTTAG 
               
               
                   
                   
                 TACAAGTATTTCTCACACTATTGTACAATTCCTACAATT 
               
               
                   
                   
                 AAAACTATACAATATTCCTGATCACCCTGCTAACTTCA 
               
               
                   
                   
                 CCCATCTTTCT 
               
               
                   
               
               
                 275 
                 F2: 75,210,562 
                 ACGCAGACAGAGTTACTGGGCCCCAAAGCCACAACC 
               
               
                   
                   
                 CGGCTTCCCTTCCTTCTCCCTTTGCCTTCTGTCAAGTT 
               
               
                   
                   
                 TTAATTCAATTAAATTAGGGAGAAACYGGGGCACCTG 
               
               
                   
                   
                 GCCTAGCTCAGTCGGTCAAGGGTCCCGCTTCGGCTC 
               
               
                   
                   
                 CGGTCACGATGTTACGGCGACATACACAGGGCTGGC 
               
               
                   
                   
                 AAAGGTGAGCCTTCCCGC 
               
               
                   
               
               
                 276 
                 A3: 14,410,638 
                 AATCATTAAAAATGATGAAATTGGAGAATACTTCATGA 
               
               
                   
                   
                 CACAGGAAAATGCAAAATCATCTTAATTGAAAAAGCAT 
               
               
                   
                   
                 TTCTTCAATATATATGTGTAGTTCRTAATCAGGGGAAA 
               
               
                   
                   
                 AAACCAGTCCCATAAAATTCTCCTATTCCAAAAGAAAC 
               
               
                   
                   
                 ATCACATCATAGAACATAGGGCCCTTCCCTCTTTTCCT 
               
               
                   
                   
                 GAGAGCTTCAA 
               
               
                   
               
               
                 277 
                 F1: 33,007,663 
                 CCATTCATTTCCTCTTCAGTGCAGGGAACCACCCAAT 
               
               
                   
                   
                 CAGACAGTTGCACAATGAAAAGAAGGTCTGAGAATGC 
               
               
                   
                   
                 CAAGGTGTGTCACTGGTTGTGGTCCCRCAGCCAGAA 
               
               
                   
                   
                 AGGGCAAAGGTGGATTGCCAAACCAGGGCTACGTAA 
               
               
                   
                   
                 CTTCAGCTGAGAAACCCTGCTGCCTTCAGGCCTGCAA 
               
               
                   
                   
                 TTTCTCTAGACCTCAGTT 
               
               
                   
               
               
                 278 
                 A1: 208,380,043 
                 TTTCAGCAAGCATTTACTAAGTGTCTGTGATGTAAGG 
               
               
                   
                   
                 CTGGTTAGGTTCTGAGTGTAGGAAATAAACTGGACGT 
               
               
                   
                   
                 GGCATCCTTAAGGAACTCATGATCCARGGTATCATTA 
               
               
                   
                   
                 ATCCCCAAAGCAGTAAGAAAGAGGCCATTGCAGAATA 
               
               
                   
                   
                 GGGGGTGGGGGTGGGCAACACCTGGAAGTTGAAGA 
               
               
                   
                   
                 CCAGGTCAGGAAAGGTCA 
               
               
                   
               
               
                 279 
                 E2: 35,480,527 
                 TTCCGACTCACATCATCCTCCACTGATGTCCTAATAGA 
               
               
                   
                   
                 GGGCACTGTGCTTTGGTCATACATGAGTTTTGATCAA 
               
               
                   
                   
                 GAGTTATATTTTCTAGTTAAAATGASAAACTGTAAAAC 
               
               
                   
                   
                 TGGATATGAGGCCTTCAGCTGTATTTACTAATTAATCA 
               
               
                   
                   
                 TACTGAGTTTTGATCCAGATGTGGGAGGAACTGAAAA 
               
               
                   
                   
                 TTCCCCTGTGTAA 
               
               
                   
               
               
                 280 
                 A3: 118,999,155 
                 ATAGTGGTAATAATATTATTAATCTTGTAGAATGCTTAA 
               
               
                   
                   
                 GTAGATTGGTTTTTTTGTTTTCTTGTTTTTGTTTTTGTT 
               
               
                   
                   
                 TTTGTTTTACATACATAAGCCTSTTAGCATAGTACCTG 
               
               
                   
                   
                 GTATACATTTCTGTATTCAAGGAATCATGTCAATTGTC 
               
               
                   
                   
                 TATTTATGTATAGTAACATAGACTCTGGGCCCATCCCT 
               
               
                   
                   
                 TCTCTTTCA 
               
               
                   
               
               
                 281 
                 A2: 10,913,767 
                 CTCTGCCACAGCACGGATGAGTCTTGAGGACGCGGT 
               
               
                   
                   
                 GCCGAGTGCAGGAAGCCTCGGAAAGGTGCTTGCCAG 
               
               
                   
                   
                 GGGCTGGAGGCGGGGGAGGTGGGGAGTGRCTAATC 
               
               
                   
                   
                 AGCATCCCTCAAGTTTCGGCCAAGCAAGATGAATGAG 
               
               
                   
                   
                 CTCTAGAGACGCGCTATATACAGCACTGTGCCTGGAG 
               
               
                   
                   
                 TCGACGGTAATGCTTTGTGC 
               
               
                   
               
               
                 282 
                 B2: 47,659,161 
                 ATTCCAGTGGCTGGTGTCATTATAGGAGGCCACGCG 
               
               
                   
                   
                 AAGACACAGAGACAGAGAAGACCACCATGTGACCAC 
               
               
                   
                   
                 GGAGGCAGCAGTTGGAGCTCCCTGCAACMAGTCAAG 
               
               
                   
                   
                 GAACGTCAGAAACCACCAGAAGCTGGAAGAGACAGG 
               
               
                   
                   
                 AAGGATTCTTAGAGCCTCCGGAGGGAGTGTGGCCCT 
               
               
                   
                   
                 GCCAACATCTTGATGTCAGAA 
               
               
                   
               
               
                 283 
                 D2: 89,706,040 
                 ATCCGTGTCACGTTCAGTCCTCATGACCGCTTTGGTC 
               
               
                   
                   
                 TGCCCTCAGCCTCGCTCCCACCTTTGGGCTCTGAACA 
               
               
                   
                   
                 CCCATCAGGAGGCTCTGCTCGGATGGYGTGAGTGTT 
               
               
                   
                   
                 CCGAGATTTGAGGCATCATCAAAACGGTCAATTACAC 
               
               
                   
                   
                 AAGTCTGGTAAGAACGCAGCTGTTTGCTTTACTTTCAA 
               
               
                   
                   
                 AAGTCTTTATTAGGGG 
               
               
                   
               
               
                 284 
                 Un12: 7,317,515 
                 TAGCCTTGACTCCTGGTTATTTTCACATAGGCTGCCT 
               
               
                   
                   
                 GCATTTGATATTATCCTCAGAAAGTCTCGCTTTTACAT 
               
               
                   
                   
                 TTTCGCACATAGATATCATCCCTTCYCATTAAAGTGCT 
               
               
                   
                   
                 CTGATGACACTTCTGTGTTTGTTTATTAGAGCTCAGCA 
               
               
                   
                   
                 GGAATTATGAGAGAAGTCGTTTTAAGAAAAGAAAAAA 
               
               
                   
                   
                 AAAACAACCTTTT 
               
               
                   
               
               
                 285 
                 Un2: 523,114 
                 CCGGAATGAGGCAAGCCTGGTACTGAGAGCAGATCC 
               
               
                   
                   
                 CTGAAGCCTGGATGGGCAGAGCTTGGTGTAAACAAAT 
               
               
                   
                   
                 TAAGTAGTGAAAGTCTGTGGAGCACTGRTTCTTATAG 
               
               
                   
                   
                 GTGGATGGACAAATGTTTATGCTGGGAGGCTGGGGA 
               
               
                   
                   
                 GGAAAATGCCACCTGACAGCTCCTTTGTTCCTGGAGG 
               
               
                   
                   
                 GGTCTCCCAGTTATCTCT 
               
               
                   
               
               
                 286 
                 B2: 156,308,475 
                 AGCGGCTGAATAATAGGTGTTTTTTTATGGATCATTGA 
               
               
                   
                   
                 AGGTAGGGGCTGCTGATGCCAGGGTAGACCGAGGTT 
               
               
                   
                   
                 TGTTTGAAGCCAATGTTCTGGAACATRTTTGGGATCT 
               
               
                   
                   
                 GACTCTTCTGAGATGTGATCAGGTGTTTGGGAGCCTA 
               
               
                   
                   
                 GGACAAAAACTCAGAGAATGATGAACTTTCTTGCTTC 
               
               
                   
                   
                 CCTCTTAACAGTGGGA 
               
               
                   
               
               
                 287 
                 A2: 25,685,296 
                 TGTTCTCTCTTCTGTCCACATATCGATCCAGGACTATG 
               
               
                   
                   
                 GTAGGAGTCGACTCATTGCCTTCTCAAACAGGGGTGT 
               
               
                   
                   
                 CACGGTCAGGATTTGGAATGACAGTYCAAGGGGCCT 
               
               
                   
                   
                 CCAGCTTTGCCATTGCTGCAGGTTAGGTCGGGAAGC 
               
               
                   
                   
                 TGCGGACTCTGTGACTGAGATTACCATTCAGGACATT 
               
               
                   
                   
                 TAATAGGGGGTGATTTG 
               
               
                   
               
               
                 288 
                 A2: 2,129,037 
                 TATCCGGGGTGTCTGGGAGAGGCGTCTTTGAAGAAG 
               
               
                   
                   
                 TTTACATTGGAGATGAGATCTGGAGGATGCGAGGGT 
               
               
                   
                   
                 GGGGAGAGGAACAGAGATCCTGAGGATAYGGATTTG 
               
               
                   
                   
                 AGTGTGTGTGTGTGTGTGTTGGAGTATTCAATGGCTC 
               
               
                   
                   
                 CCTATTGTCCTCACAATAACACCCAAACTCCTCCCTCT 
               
               
                   
                   
                 TTCCAGGGACAGAGGGAC 
               
               
                   
               
               
                 289 
                 A2: 161,801,210 
                 TTAATAAATTGAGTCAAATAATTCTCCCTCTCTTGTCT 
               
               
                   
                   
                 GAGCCAGTGCTTTTCTGCTTGAGGAATGAGTAGCTTA 
               
               
                   
                   
                 GATGATTGATAACAGAATCCATAACMTTTCCCCTCCA 
               
               
                   
                   
                 AGTCACCAGCTTGAACCCAACCTAGTTGAGCAATGAG 
               
               
                   
                   
                 AGACATTTGTTTCCCCAGGCAGCTTATGAGAGGTTTG 
               
               
                   
                   
                 CATGAAATGAATGGG 
               
               
                   
               
               
                 290 
                 D3: 7,290,581 
                 CCAACCACGATATAATTGCACTCATCCAAAAACAGAA 
               
               
                   
                   
                 GTGACCGGGGCCACAGTAAATGTGCCTCTTTTGCAAC 
               
               
                   
                   
                 TCTCATTCCTCTTAAATCTCAAATAARGATTAAAAATGT 
               
               
                   
                   
                 GCATTTGAGGAGGGCCACCGTGGTGGCTCAGTCGGT 
               
               
                   
                   
                 TAAGTATCTGACTCTTGATATCGACTCAGGTCATAATC 
               
               
                   
                   
                 TCATGGTTTGTGAG 
               
               
                   
               
               
                 291 
                 F1: 19,516,618 
                 AGCACAGCTGGGGATCTTCCATCCCGGTGCTGTTTCC 
               
               
                   
                   
                 AGCAGCTCGGTGCGGAAGCACCACCTTTCTGGCTTG 
               
               
                   
                   
                 TAAACTGAGAATACTGATCCAGCCCCCRTGAAGAGAC 
               
               
                   
                   
                 ATTACCTAAGAAACCTCCCTTATGAAGCCTTCAAGGT 
               
               
                   
                   
                 GAGAGTATTTTACAGGGAAATCCACAGGCTAAAAATA 
               
               
                   
                   
                 AAAACACAACTATACCT 
               
               
                   
               
               
                 292 
                 D2: 82,189,281 
                 AGGAAAAGGAGAACATTAACATGATTCTTGGAGGTTC 
               
               
                   
                   
                 AACGGTGTTAAGTCCAACCCCTCACAGGACTCCACGC 
               
               
                   
                   
                 ATCCCTTAGCAGGAGTTAAGGGAGAAYGGTAACCCTC 
               
               
                   
                   
                 ACAGTAGGACAATCCCCCTGCAGACCTCCTCCATCAT 
               
               
                   
                   
                 CAAGGTCAAGTGGGAACTCCAGAACTCTGAGTGTTTT 
               
               
                   
                   
                 GGCAAGATGCTCCCTC 
               
               
                   
               
               
                 293 
                 C1: 36,295,835 
                 GAGATGAATGACTGCCCTGAGTGCCAACAACAGAAA 
               
               
                   
                   
                 GTGCATAGGATAAAGAGAGAGTTGACACAGGAACTCA 
               
               
                   
                   
                 TACCCCTGAGGTAACATACCATACACCRAAAGTTAGC 
               
               
                   
                   
                 AGTGGACAATAAGTAGCTTAGAGCCCTGAACTTGTCC 
               
               
                   
                   
                 TGTCATATATCCAATGTCAGCACCACTCTGGAAACAC 
               
               
                   
                   
                 AATCCCACATAATCCCC 
               
               
                   
               
               
                 294 
                 D3: 33,258,191 
                 ACACCAAAGGACAAAGGCCAAGGGCAGGCTTACTGG 
               
               
                   
                   
                 CTTGGATGAGTGATGGAGGGCTGCTCTTAGGGAACA 
               
               
                   
                   
                 CGGGGCTGGGGCGAGGGCCGGTGACACARTGTGTG 
               
               
                   
                   
                 ATCAGGCGGGGCTTTCCAGCAGCCTCAATGCTGAGG 
               
               
                   
                   
                 GGGGCAGGAGGCCAAAGGCAGCGCCTTAAGAAGCA 
               
               
                   
                   
                 GGCCAAACAGGGGCATCTGGGTG 
               
               
                   
               
               
                 295 
                 C2: 63,676,887 
                 ACTAAATCATGTGCTTCTTCTATGAGGACTCCAAATGA 
               
               
                   
                   
                 GTTCATAGCTTACTCATCACTAACATGAGCACCACACT 
               
               
                   
                   
                 AGGTAGTTCGTATACTGTTTCATTYGACTCTAATGGCC 
               
               
                   
                   
                 TTATAAAGTAGATGTAATCATTATCCCACTTTGCAGAG 
               
               
                   
                   
                 GGAAAAAACAGAAACTTAGCTTAAGCAATTTGCCTGTT 
               
               
                   
                   
                 TACATTACTAG 
               
               
                   
               
               
                 296 
                 A3: 48,181,817 
                 CCCTCCCCCACTCTCGCTATACGTGTGTGTCTCTCTC 
               
               
                   
                   
                 TCAAAAATAAACACTAAAAAAATGTTTTAAAAACACAA 
               
               
                   
                   
                 CGTGGTTAATTCATGTGGAATTCCTYATGTTACAAGTT 
               
               
                   
                   
                 ATTTGCAAAATTTTTTTCTTCTTCTTCTCATTCAGTTTT 
               
               
                   
                   
                 ACCTGGGACTGGACGGAGCCAGACATTTGTGATCCA 
               
               
                   
                   
                 AGCTTCTACTACA 
               
               
                   
               
               
                 297 
                 A3: 11,904,341 
                 TTTCCCATGAGACTCATGCTCTAAAAGGAGATGCAGA 
               
               
                   
                   
                 CCCAAGTGTGAGGAATGGAATGACGAAGACTGAAGC 
               
               
                   
                   
                 ACCCTTTTTTTGAGAGAGAGAAAGGGCRGGGGGGGG 
               
               
                   
                   
                 GTGTACTTTAAGCAGTTTCCATGCCCAGCACAGACCC 
               
               
                   
                   
                 CAACGCGGGGTTCAATCCCCTGACCTTGAGATCATGA 
               
               
                   
                   
                 CCTGAGACAAAACCAAGA 
               
               
                   
               
               
                 298 
                 E3: 63,458,569 
                 GTTCTTTGCTGAGCATAAAGGATTGGCTATGGGGAAT 
               
               
                   
                   
                 TTTCTTTTATCTTTGAAAATCTGTTGCGTATCTTTTAGA 
               
               
                   
                   
                 AATAGTTTTTACCTGGTTTTCTCTMTTTTTGTTTAATTT 
               
               
                   
                   
                 TTTTTTCTTTTGGGGGGAATTATATGGGGCGGAAGTT 
               
               
                   
                   
                 TTATACCAAGAGGCACACAGCATATTCTATGTTAGCAT 
               
               
                   
                   
                 TGACTCCTCTT 
               
               
                   
               
               
                 299 
                 B2: 112,716,268 
                 TTGTTTCTATCTAATTTTTTATCTTCTTCCCTGATACTT 
               
               
                   
                   
                 AATTTTTATTTGTCTTTTATCTCCAACTGTCTGTAATAG 
               
               
                   
                   
                 TTACTTCCAAAAAAAAAATCAAWCATGTTTAAAACAGA 
               
               
                   
                   
                 ATTCACAGCCCACTAACCCCAAATAACCCCAAAACAT 
               
               
                   
                   
                 AGCCCTCAACTGTTTCATTAAGAAGATGTTCTCTGGA 
               
               
                   
                   
                 GTCAAAAAGAA 
               
               
                   
               
               
                 300 
                 B3: 149,673,110 
                 ACAGCGGGCTCTGGGGCTCGAGCTGGCTCCCTTGGT 
               
               
                   
                   
                 CACACGCAGCTTTCCATGATGCTTCCAGTTCTCCAGA 
               
               
                   
                   
                 ACTCTCCGATTGATCCTGCCCTCCCCAYGGGGCAGA 
               
               
                   
                   
                 GCGTCCACTCCTGGTGACTCCTGGTCCCCTGTATCTG 
               
               
                   
                   
                 TGCCAACGGGGGCCGGTGGCGGGGGGGGAAGCGG 
               
               
                   
                   
                 CGTGGCTTGGCTGGAGGGGTA 
               
               
                   
               
               
                 301 
                 C1: 125,311,520 
                 GGGTTTTGGGCTTGTCATGGGTAAACAAGGGAGGCA 
               
               
                   
                   
                 TCTAAGGTGGTTCTGTGCAGTAAACCATTTCCAGGAA 
               
               
                   
                   
                 CACAAAAGGGCGGGGGGAGTTTTTTAGRAAAAATAAT 
               
               
                   
                   
                 TAATGTTTATTTTTCAGAGAGAGAGACAGAGAGACAG 
               
               
                   
                   
                 AGGGTGAGTGGGGGAGGGGCAGAGAGAGAAGGAGA 
               
               
                   
                   
                 TACAGATCTGAAGCAGGCT 
               
               
                   
               
               
                 302 
                 B1: 19,312,704 
                 GACCCTGTGTGAGGTTTTAAGCCTGGTTTCTCTACTC 
               
               
                   
                   
                 ACTAGTTGTGTGACCTTGAGAAACCAACTTAATCTCAA 
               
               
                   
                   
                 CCGCATATGGTTGTAGAGAGAATTAYGTAAGATAATA 
               
               
                   
                   
                 AAAAATTGAGAGCACTGCCGGGCATGTAAAAGCTCAA 
               
               
                   
                   
                 TAATATTAAATGTTGTCATTGCTATTGTCATTAATACTG 
               
               
                   
                   
                 GCCAGGATCCAGC 
               
               
                   
               
               
                 303 
                 B2: 120,276,458 
                 GGGAATCAAGGAAAACCCAAAGTACTAAATGGATATT 
               
               
                   
                   
                 ATAAATATAGGAGATGAATTTTTTTTAGTAGATATATAT 
               
               
                   
                   
                 AGAGAGCTGATAAATGGCAAGGGTSTAAAAGAACCAA 
               
               
                   
                   
                 TGAGTATAATAAAAAATATTTCTTAGCAAATAAAGTATT 
               
               
                   
                   
                 TAGATGTTTGAACAGTGCTTTTCAATTTTTTATTGATTA 
               
               
                   
                   
                 ATTTGACAAT 
               
               
                   
               
               
                 304 
                 B2: 159,389,942 
                 AATGACCCCTTTCTATTTGACAGAATTCACATCAAAAG 
               
               
                   
                   
                 CCAATGAGATGAGGCCAGGAGTTTGCTTTCCCTGTTG 
               
               
                   
                   
                 TGAAGGTGGGAGGGGAACCAGCAGCRGTGATAAGTG 
               
               
                   
                   
                 GCTGTGATCCCTCCAACCTTTGCCAAGGTGAACCTCC 
               
               
                   
                   
                 ACCCACCCCCCTCACCGTGTCTCAGATAGAAATGCTT 
               
               
                   
                   
                 GTTTCTGATGTTTTTC 
               
               
                   
               
               
                 305 
                 D4: 39,362,745 
                 ACAAATTTCATTGCATTTGAGAAAAGCGCTGTGCTGG 
               
               
                   
                   
                 GGAAGACCTTTTTGTTTTTGGAACACACTACTAGCATG 
               
               
                   
                   
                 GTGAGCCTCACGGAGTCTTTGCTTAYGAACGTATAAA 
               
               
                   
                   
                 TATGCTTGTAGGTCAATGGCATCATACCAGAATACAC 
               
               
                   
                   
                 TGCAATAGAAGCACATCTTTCCTCGTATTAAAAGGATA 
               
               
                   
                   
                 GGTATCTGTGCATA 
               
               
                   
               
               
                 306 
                 B1: 172,534,764 
                 AAAATAATCAAAATCTGGGGCGCCTGGGTGGCGCAG 
               
               
                   
                   
                 TCGGTTAAGCGTCCGACTTCAGCCAGGTCAGAATCTT 
               
               
                   
                   
                 GAGGTCCGTGAGTTCGAGCCCCGCGTCRGGCTCTGG 
               
               
                   
                   
                 GCTGATGGCTCAGAGCCTGGAGCCTGTTTCCGATTCT 
               
               
                   
                   
                 GTGTCTCCCTCTCTCTCTGCCCCTCCCCCGTTCATGC 
               
               
                   
                   
                 TCTGTCTCTCTCTGTCCC 
               
               
                   
               
               
                 307 
                 POINTED1 
                 TTAGCCGATTGGAGGAGTACAATAGCCGTCAGGCTTT 
               
               
                   
                   
                 ATGTGATGGAACTCCAGAGGGACCATTACTGCGCAAT 
               
               
                   
                   
                 CCC [G/A] GAAACCATGACAAAGCCAGGACCCCAAGGC 
               
               
                   
                   
                 TCCCCTCCTCTGCTGATGTGGAATTTTGCCTAAGTCT 
               
               
                   
                   
                 GACACAATATGAATCGGGTTCCATGGATAAAGCTGCA 
               
               
                   
               
               
                 308 
                 POINTED2 
                 ACACTGCTTGGAGGGTCTGAAATCTGGAAAGACATTG 
               
               
                   
                   
                 ATTTTGCTCATGAAGCCCCTGGTTTCCTGCCTTGGCA 
               
               
                   
                   
                 CAGACTCTTCTTGTTGCTGTGGGAACAAGAAATCCAG 
               
               
                   
                   
                 AAGCTGACC [G/T] GGGATGAGAACTTCACTATTCCATA 
               
               
                   
                   
                 TTGGGATTGGCGAGATGCTAAAAGCTGTGACA 
               
               
                   
               
               
                 309 
                 ALBINO 
                 TTAGCCGATTGGAGGAGTACAATAGCCGTCAGGCTTT 
               
               
                   
                   
                 ATGTGATGGAACTCCAGAGGGACCATTACTGCGCAAT 
               
               
                   
                   
                 CCCGGAAACCATGACAAAGCCAGGACCCCAAGGCTC 
               
               
                   
                   
                 CC [C/*] TCCTCTGCTGATGTGGAATTTTGCCTAAGTCT 
               
               
                   
                   
                 GACACAATATGAATCGGGTTCCATGGATAAAGCTGCA 
               
               
                   
               
               
                 310 
                 CHOCOLATE 
                 TGACCCTGCTATTCGAAGCCTTCACAATTTGGCTCAT 
               
               
                   
                   
                 CTATTCCTGAATGGAACAGGGGGACAAACCCATTTAT 
               
               
                   
                   
                 CTCCAAACGATCCTATTTTTGTCCTCCTGCACACTTTC 
               
               
                   
                   
                 ACTGACGCAGTCTTTGATGAATGGCTGAGGAGATATA 
               
               
                   
                   
                 ATGCTGGTGA [G/A] ACATTTCCTATGTTAACAAGATGT 
               
               
                   
                   
                 CTTTGGCATATTTTAGATGTATCCACATTTCCATTGGA 
               
               
                   
                   
                 AAATGCCCCTATTGGACATAATAGGCAATACAATATG 
               
               
                   
                   
                 GTGCCATTCTGGCCTCCAGTTACCAACATAGAAATGT 
               
               
                   
                   
                 TTGTTACTGCTCCAGACAAACTGGGATATACTTATGAA 
               
               
                   
                   
                 GTTCAATGGCCAAGTGAGTATTGAAAATGTATCTTTTC 
               
               
                   
                   
                 TGTGGAAATTACCAAAACTACATTTGCTACCTTTTAAG 
               
               
                   
                   
                 GTAATGACAG 
               
               
                   
               
               
                 311 
                 CINNAMON 
                 CAGGTGTGAGGCAGTGACTGCAGACTCACGACCCCA 
               
               
                   
                   
                 CAGCCTCCATTACCCGCATGATGGCAGAGATGATCG 
               
               
                   
                   
                 GGAGGCTTGGCCCACGAGGTTCTTCAACAGGACATG 
               
               
                   
                   
                 C [C/T] GATGCAATGGCAATTTCTCAGGACACAACTGTG 
               
               
                   
                   
                 GGACTTGCCGTCCTGGATGGAAAGGAGCTGCTTGTG 
               
               
                   
                   
                 ACCAGAGAGTTCTCATAGGTAAGTGGGGATCTGCATG 
               
               
                   
                   
                 TACATACAGTTCTTCATGAGACTCTATGCATTTAATAG 
               
               
                   
                   
                 GAACCTAAATCATTTGAACTGGAAGCACATCTGAAAAT 
               
               
                   
                   
                 CATACAAC 
               
               
                   
               
               
                 312 
                 Mucopolysaccharidosis 
                 GCTGTGGCTGTTGGTTTCCTCCGCCGTCTCCATACAA 
               
               
                   
                 Type VI 
                 CGATTCTGCGATACCCTCATCAGACCCACCGACCAAG 
               
               
                   
                   
                 ACCCTCTGGC [T/C] CTTTGATATTGATCAGGACCCAGA 
               
               
                   
                   
                 AGAAAGACATGACCTGTCAAGAGACTATCCCCATATT 
               
               
                   
                   
                 GTCGAGCAGCTCCTTTCCCGCCTCCAGTTCTACCACA 
               
               
                   
                   
                 AACATTCAGTGCCTGTGCATTTCCCGGCACAGGACCC 
               
               
                   
                   
                 CCGCTGTGACCCCAAG 
               
               
                   
               
               
                 313 
                 Mucopolysaccharidosis 
                 CATGACCTGTCAAGAGACTATCCCCATATTGTCGAGC 
               
               
                   
                 Type VI MILD 
                 AGCTCCTTTCCCGCCTCCAGTTCTACCACAAACATTC 
               
               
                   
                   
                 AGTGCCTGTGCATTTCCCGGCACAG [G/A] ACCCCCGC 
               
               
                   
                   
                 TGTGACCCCAAGGGCACTGGGGCCTGGGGCCCTTG 
               
               
                   
                   
                 GGTATAG 
               
               
                   
               
               
                 314 
                 Polycystic Kidney 
                 TTCTTCCTGGTCAACGACTGGCTGTCGGTGGAGACTG 
               
               
                   
                 Disease 
                 AGGCCAATGGCGGCCTCGTGGAGAAGGAGGTGCTG 
               
               
                   
                   
                 GCAGCAAGTAAGGGCCTGGGCCCGTCCCTGCCCGG 
               
               
                   
                   
                 GCTGGCCGAGGGGTGGCCTGTGCCACTGGCCTCCT 
               
               
                   
                   
                 GAAGCCAGCTGTGCCCTTTCTGCAGGCGACGCGGCT 
               
               
                   
                   
                 GTGCGGCGGTTCCGGCGCCTCCTGGTGGCCGAGCT 
               
               
                   
                   
                 GCAGCGTGGCTTTTTTGACAAGCATCTCTGGCTCTCC 
               
               
                   
                   
                 CTCTGGGACCGGCCTCCTCGGAGCCGCTTCACCCGC 
               
               
                   
                   
                 GTCCAGCGGGCCACCTGTTG [C/A] GTCCTCCTCGTCT 
               
               
                   
                   
                 GCCTCTTCCTGGGCGCCAATGCTGTGTGGTACGGGG 
               
               
                   
                   
                 TCGTGGGAGACGCCGCCTACAGGTGGGTGCCCGAG 
               
               
                   
                   
                 GGGGGCCCGATGATCTCCTCCTGCCCGACCCCTCCT 
               
               
                   
                   
                 ACCCCCCACAGCCTCTCCCAGCCCGGGTCTCTCTCC 
               
               
                   
                   
                 TCTCCTGCCACACAGCGCGGGGCCCGTGTCCGGTCT 
               
               
                   
                   
                 GATCCCGCTGAGTGCCGACACAGTTGCCGTCGGCCT 
               
               
                   
                   
                 GGTGTCCAGTGTGGTCGTCTATCCCGTCTACCTG 
               
               
                   
               
               
                 315 
                 Hypertrophic 
                 CTCAGCCTTCAGCAAGAAGCCAAGGTCAGTGGAAGT 
               
               
                   
                 cardiomyopathy MC 
                 GGCAGCCAGCAGCTCTGCTGTGTTCGAG [G/C] CCGA 
               
               
                   
                   
                 GACAGAGCGGTCAGGAGTAAAGGTGCGCTGGCAGC 
               
               
                   
                   
                 GGGGGGGCAGTGACATCAGCGCCAGTGACAAGTATG 
               
               
                   
                   
                 GCCTAGCAGCCGAGGGCACGAGGCACACTCTGACAG 
               
               
                   
                   
                 TGCGGGACGTGGGCCCCGCCGACCAGGGACCCTAC 
               
               
                   
                   
                 GCAGTCATCGCTGGCTCCTCCAAGGTCAAGTTTGACC 
               
               
                   
                   
                 TCAAGGTCATAGAAG 
               
               
                   
               
               
                 316 
                 Hypertrophic 
                 GGCTACATCCTGGAGCGCAAGAAGAAGAAGAGCTTC 
               
               
                   
                 cardiomyopathy RG 
                 CGGTGGATG [C/T] GGCTGAACTTTGACCTGCTGCAGG 
               
               
                   
                   
                 AGCTGAGCCACGAGGCACGGCGCATGATTGAGGGC 
               
               
                   
                   
                 GTGGTGTATGAGATGCGAGTCTACGCGGTCAATGCC 
               
               
                   
                   
                 ATCGGCATGTCCAGGCCCAGCCCTGCCTCCCAGCCC 
               
               
                   
                   
                 TTC 
               
               
                   
               
               
                 382 
                 MLPH DILUTION 
                 atggggaaaaaactggatctttccaagctcacggacgacgaggccaagcaatctggg 
               
               
                   
                   
                 aagtggttcagcgggactttgatctgagaaggaaagaagaggaaaggctggggggat 
               
               
                   
                   
                 tgaaggacaggattaagaaagagagctcccagagggagctgctctcggatgcggccc 
               
               
                   
                   
                 acctgaatgagacccactgcgcccgctgcctgcagccctaccggctcctcgtggccccc 
               
               
                   
                   
                 aagaggcaatgcctggactgtcacctcttcacctgccaagactgtagccacgcccaccc 
               
               
                   
                   
                 ggaggaggagggctggctctgtgacccctgccacctggccagggttgtgaagatgggc 
               
               
                   
                   
                 tcactggagtggtactacgggcacctgagagcccgcttcaagcggtttgggagcgcca 
               
               
                   
                   
                 aagtgatccggtccctgtgcgggcggctgcagggtggaggtgggcctgagccaagcc 
               
               
                   
                   
                 ctggagagggaagtggagacagtgagcagacagaagaggatggagaactggaca 
               
               
                   
                   
                 cagtggcccaggcccaaccccttgggagcaaaaaaaagcgcctctccattcacggctt 
               
               
                   
                   
                 ggactttgatgcagactctgatggctcgactcagtccggcggtcaccccccatatctgtcc 
               
               
                   
                   
                 ccggtccccatggccacagacagcctgcaggccctcacaggtgaatcccgtgccaag 
               
               
                   
                   
                 gacacctcccaggaggccgtggtcctggaagaggctgatgtcggtgcccctggactcc 
               
               
                   
                   
                 accctcatccagaagagcagacagacagcctctcagctgccagacaggacaccctca 
               
               
                   
                   
                 ctgagccccgcttccccagacagtcctgcacaacagccctggggttggctgtcacaccc 
               
               
                   
                   
                 ggtccaggcgtcatcagcagtagtgagcggctctcctcccggtacccggctgacgaag 
               
               
                   
                   
                 gcacctccgatgacgaggacaccggggctgacggtgtggcctcccagagcctcacgt 
               
               
                   
                   
                 ggagggactgcgccccggctgagagccagcatctcaccggccaccagcccacagac 
               
               
                   
                   
                 gccgacagagaagaagagaccctaaagaggaagctggaggagatgaccagccac 
               
               
                   
                   
                 atcagtgaccagggggcctcgtccgaggaggaggggagcaaggaggaagaggca 
               
               
                   
                   
                 ggactgaacaggaaaacctccatcgaggacctccccggggcagccccagaggtgct 
               
               
                   
                   
                 cgtggcttcgggccaaacgtccagacaggaaacaagtccccggggtcctcaggaact 
               
               
                   
                   
                 catgcagcccggcagaaccacggaccaggagctgctggagctggaagacagagtg 
               
               
                   
                   
                 gccgtgacggcctctgaggttcagcaggtggagagtgaggtttctaacatcaagtccaa 
               
               
                   
                   
                 gattgccgccttgcaggctgccgggctctcggtgagaccctcgggaaagccccagcgg 
               
               
                   
                   
                 aggtccaacctcccgatatttcttccccgactcgttgggagattgggccagacccctaag 
               
               
                   
                   
                 gatccaaacgcagagccttcggatgaggtcaaggtgatgactgcaccctaccttctgag 
               
               
                   
                   
                 aaggaagttcagtaatcccccaaaaagtcaagataaggctggcgactcctttgaccgg 
               
               
                   
                   
                 cagtcagcgtaccgcggatccctgacgcagagaaaccccaacagcaggaagggagt 
               
               
                   
                   
                 ggccaaccacagctttgcaaaacccgtgatgacccagcggccctga 
               
               
                   
               
             
          
         
       
     
         [0083]    In further embodiments, the present invention may be used to identify characteristics associated with cattle, multi-breed and the like. For example, the following tables demonstrate sequences that may be used determined genetic characteristics, such as parentage, identity, sex, genotype and/or phenotype and breed determination in cattle and the like. Thus, in further embodiments, the present invention provides a panel comprising a plurality of assay compositions, wherein each assay composition is capable of identifying at least one of the nucleotide markers as set forth in Tables 9-11 provided below. Further information for sequences provided herein may be identified by searching appropriate genetic databases. Table 9 provides allele variations between allele 1 and allele 2 to assist those skilled in the present art and the approximate location in centiMorgans of the centromere as used by those skilled in the present art. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 CATTLE AND MULTI-BREED SNP PANEL SEQUENCES 
               
               
                 T 
               
             
          
           
               
                   
                   
                   
                 Approx. 
                   
                   
                   
               
               
                 SEQ ID 
                 SNP ID* 
                 Chr 
                 location %   
                 Allele 1 
                 Allele 2 
                 Description 
               
               
                   
               
             
          
           
               
                 317 
                 MBS042-1 
                 2 
                 28.6 
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 318 
                 MBS029-1 
                 2 
                 107.3 
                 A 
                 G 
                 SNP Marker in many breeds 
               
               
                 319 
                 MBS048-1 
                 4 
                 80.7 
                 G 
                 C 
                 SNP Marker in many breeds 
               
               
                 320 
                 MBS007-1 
                 5 
                 41 
                 C 
                 A 
                 SNP Marker in many breeds 
               
               
                 321 
                 MBS030-1 
                 5 
                 108.3 
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 322 
                 MSB043-1 
                 5 
                 129.2 
                 G 
                 T 
                 SNP Marker in many breeds 
               
               
                 323 
                 AH2-5 
                 6 
                 83.5 
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 324 
                 MBS044-1 
                 7 
                 28.2 
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                 325 
                 MBS014-1 
                 8 
                 73.6 
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 326 
                 MBS031-1 
                 10 
                 33.5 
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                 327 
                 AH8-4 
                 11 
                 56.6 
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 328 
                 MBS015-1 
                 11 
                 130.5 
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 329 
                 AH25-1 
                 13 
                 50.7 
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 330 
                 MBS046-1 
                 13 
                 94.6 
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 331 
                 MBS047-1 
                 16 
                 78.9 
                 G 
                 T 
                 SNP Marker in many breeds 
               
               
                 332 
                 MBS018-1 
                 17 
                 78 
                 G 
                 T 
                 SNP Marker in many breeds 
               
               
                 333 
                 MBS020-1 
                 17 
                 106.4 
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 334 
                 MBS033-1 
                 18 
                 55 
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                 335 
                 MBS021-1 
                 18 
                 62 
                 T 
                 G 
                 SNP Marker in many breeds 
               
               
                 336 
                 AH13-4 
                 19 
                 34 
                 A 
                 G 
                 SNP Marker in many breeds 
               
               
                 337 
                 MBS034-1 
                 19 
                 45.3 
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 338 
                 MBS054-1 
                 19 
                 67.8 
                 C 
                 A 
                 SNP Marker in many breeds 
               
               
                 339 
                 MBS049-1 
                 21 
                 38.7 
                 C 
                 G 
                 SNP Marker in many breeds 
               
               
                 340 
                 MBS025-1 
                 23 
                 8 
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                 341 
                 MBS039-1 
                 23 
                 11.5 
                 A 
                 T 
                 SNP Marker in many breeds 
               
               
                 342 
                 MBS035-1 
                 23 
                 37.5 
                 A 
                 G 
                 SNP Marker in many breeds 
               
               
                 343 
                 MBS028-1 
                 24 
                 69.8 
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 344 
                 MBS040-1 
                 25 
                 16.1 
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 345 
                 MBS051-1 
                 25 
                 56.8 
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 346 
                 MBS041-1 
                 29 
                 56 
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                 347 
                 421_10 
                 1 
                   
                 C 
                 G 
                 SNP Marker in many breeds 
               
               
                 348 
                 423_24 
                 10 
                   
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 349 
                 425_2 
                 9 
                   
                 A 
                 G 
                 SNP Marker in many breeds 
               
               
                 350 
                 431_a2 
                 5 
                   
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 351 
                 487_67 
                 14 
                   
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 352 
                 448_67 
                 2 
                   
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 353 
                 16_2 
                 7 
                   
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 354 
                 417_16 
                 4 
                   
                 G 
                 A 
                 SNP Marker in many breeds 
               
               
                 355 
                 486_67 
                 3 
                   
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                 356 
                 436_C10 
                 4 
                   
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                 357 
                 454_g11 
                 17 
                   
                 C 
                 G 
                 SNP Marker in many breeds 
               
               
                 358 
                 013.SP3 
                 6 
                   
                 T 
                 C 
                 SNP Marker in many breeds 
               
               
                 359 
                 018.SP6 
                 3 
                   
                 C 
                 T 
                 SNP Marker in many breeds 
               
               
                   
               
               
                 *As designated by from Heaton et al (2002) Selection and use of SNP markers for animal identification and Paternity analysis in U.S. beef cattle 
               
               
                   % Location in centiMorgans from centromere 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 CATTLE DISEASES AND TRAITS 
               
             
          
           
               
                 SEQ ID 
                 SNP 
                 DISEASE/TRAIT 
                 Allele 1 
                 Allele 2 
                 BREED 
               
               
                   
               
               
                 360 
                 BLAD 
                 (BLAD) Bovein Lymphocyte 
                 A 
                 G 
                 Holstein 
               
               
                   
                   
                 Adhesion Deficiency 
               
               
                 361 
                 DUMPS 
                 (DUMPS)—Def. of Uridine 
                 C 
                 T 
                 Holstein 
               
               
                   
                   
                 Monophosphate Synthetase 
               
               
                 362 
                 CHONDR 
                 Dwarfism/chondrodysplasia 
                 * 
                 GGCA 
                 Dexter 
               
               
                 363 
                 PROTO 
                 Protoporphyria 
                 G 
                 T 
                 Limousin 
               
               
                 364 
                 HYPOTR 
                 Hypotrichosis 
                 C 
                 T 
                 Red Angus/Charolais 
               
               
                 365 
                 SYNDAC 
                 Syndactyly 
                 CG 
                 AT 
                 Holstein Angus 
               
               
                 366 
                 CITRUL 
                 Citrullinemia 
                 C 
                 T 
                 Holstein-Freisian 
               
               
                 367 
                 CVM 
                 Complex Vertebral 
                 G 
                 T 
                 Holstein 
               
               
                   
                   
                 Malformation 
               
               
                   
                   
                 COLOR 
               
               
                 368 
                 E+ 
                 Black coat color 
                 T 
                 C 
                 many 
               
               
                 369 
                 RED 
                 Red coat color 
                 G 
                 * 
                 many 
               
               
                 370 
                 DUN 
                 Dun coat color 
                 C 
                 T 
                 Dexter 
               
               
                 371 
                 ALBINO 
                 Albinism 
                 * 
                 C 
                 Braunvich, Brown 
               
               
                   
                   
                   
                   
                   
                 Swiss 
               
               
                 372 
                 ROAN 
                 Roan coat color 
                 C 
                 A 
                 Shorthair, Belgian 
               
               
                   
                   
                   
                   
                   
                 Blue 
               
               
                   
                   
                 QTL 
               
               
                 373 
                 Ucalp 
                 u-calpain SNP 
                 G 
                 C 
                 many 
               
               
                 374 
                 CALPA 
                 calpastatin SNP 
                 G 
                 C 
                 many 
               
               
                 375 
                 MYOS 
                 myostatin increased muscling 
                 C 
                 A 
                 many 
               
               
                   
                   
                 mass 
               
               
                 376 
                 ABCG2 
                 ABCG2 
                 A 
                 C 
                 Holstein 
               
               
                 377 
                 Kcasein 
                 kappa-casein 
                 A 
                 C 
                 Holstein 
               
               
                 378 
                 zfxy1 
                 Zinc Fingers X + Y 
                 T 
                 C 
                 Many 
               
               
                 379 
                 zfxy2 
                 Zinc Fingers X + Y 2 
                 T 
                 A 
                 Many 
               
               
                 380 
                 GHR 
                 GHR gene 
                 A 
                 G 
                 Angus, Charolais 
               
               
                 381 
                 Bcasein 
                 Beta casein 
                 C 
                 A 
                 Many 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 11 
               
             
             
               
                   
               
               
                 CATTLE SNP PANEL NUCLEOTIDE MARKER SEQUENCES 
               
             
          
           
               
                 SEQ ID 
                 SNP ID 
                 SEQUENCE 
               
               
                   
               
             
          
           
               
                 317 
                 MBS042-1 
                 Ctggagtgcgtttcaaaatggaacagataaaaaactagtaagtacataagtacatatctactgg 
                   
               
               
                   
                   
                 cctttgatctgactagttccccagtctcaggtct [g/a] tttgctgttaatcaccagtgagagaaggtc 
               
               
                   
                   
                 ctaccctatct 
               
               
                   
               
               
                 318 
                 MBS029-1 
                 Tctgggagaggtacacggggtgggggaggggcgagtggctgcctcgggaggcacgggaga 
               
               
                   
                   
                 ggtgaaaagcagctgagggatcacggatgctttgaacNgggctgcaa [a/g] tagttgatacga 
               
               
                   
                   
                 agtcaccgtgattgctttcgaccggtatgatttgtacaaaccagctcaacccttg 
               
               
                   
               
               
                 319 
                 MBS048-1 
                 aaccgtgacggcatcatctgcaagtcggaccttagagagacctactcccagctcggtgagggc 
               
               
                   
                   
                 acccgtctcctgcctggcccagcccctctcacaccag [g/c] gacccggctcagagctgctgcct 
               
               
                   
                   
                 gNcccNgcctgtactcctgtcctggctcacaccacccacccacccctagaacacattccttccN 
               
               
                   
                   
                 cttcacttcccccacccagtga 
               
               
                   
               
               
                 320 
                 MBS007-1 
                 ggaagaggggcaagggagagctaaaggcctNgacgggatcagtgagagaccagccagct 
               
               
                   
                   
                 gagtgacttag [c/a] aggggaggatggagccacctccaggagagttggctNgaaaggatttct 
               
               
                   
                   
                 tctcNgcccatcgatttcctgcctcactcct 
               
               
                   
               
               
                 321 
                 MBS030-1 
                 Tcttgaaaggttgctctgccacctgctgcttaaccttctcagcccctgtggtgtttccaaagggctgg 
               
               
                   
                   
                 tcac [g/a] gtcctcaggcttgggtgtggcctgggtcttggagagggatggtgctgcgggcaagtc 
               
               
                   
                   
                 tgtgtccatg 
               
               
                   
               
               
                 322 
                 MSB043-1 
                 gagaggggaggggaggggagggttgccctcctacacctggccccacctgtacctccttcNgt 
               
               
                   
                   
                   [g/t] agcctttgttctagctagaagggcccctgaattctccaggtaacccctgagagggaaggaa 
               
               
                   
                   
                 atgcct 
               
               
                   
               
               
                 323 
                 AH2-5 
                 tgtcagataaaatacaggatgtccagttatatttgaatattaagtaaaacaatgaatattttttcagtg 
               
               
                   
                   
                 t [t/c] gcatgtacattgttccatgtaggacatgcttatattttagaaattattcattgtatactgtaaattc 
               
               
                   
                   
                 caatcga 
               
               
                   
               
               
                 324 
                 MBS044-1 
                 Cggtccatgccatgttactgtctgtaaccctgtgggcactgctagaacctcacttctgaccataact 
               
               
                   
                   
                 gaagcccaggg [C/T] gatgagaggtgatggagctctgactattaggccgcccagctctggtct 
               
               
                   
                   
                 gggttcttaaccacttctcaaga 
               
               
                   
               
               
                 325 
                 MBS014-1 
                 gcagactcagcagccactaacaaggggctgcgagccatcaaaggggtccgtggaaaagact 
               
               
                   
                   
                 gtagagagca [t/c] gggaaaggagccactatgcaggaagtcacaggaagctttgcagaaaa 
               
               
                   
                   
                 actaacatttgagctggctccag 
               
               
                   
               
               
                 326 
                 MBS031-1 
                 gtgccactccaaggtggtgaagaacaatctgaaccccagctgggagccgttccgtctgtccctg 
               
               
                   
                   
                 cactccctgtg [c/t] agctgtgaNgtccaccggcctctcaaggtgaggccccacgagtaaggg 
               
               
                   
                   
                 cagcctggtagagcagccagcctctg 
               
               
                   
               
               
                 327 
                 AH8-4 
                 gacttcagaatggaaaccctctctccctaaagaaagccatacccagggagtccacNtgggctg 
               
               
                   
                   
                 aataacccc [g/a] aggactggcagaagggaagggaagaatgtagctgcagcctgaacttca 
               
               
                   
                   
                 ctgttgtctNatccatgcccNactgcctt 
               
               
                   
               
               
                 328 
                 MBS015-1 
                 gtcagagcccaggctggtccgaggccgcacccgctggcctccctgccccgtgagagggggag 
               
               
                   
                   
                 gcaggaacatcccatc [t/c] ggaagtagccgctcttccaagtctggaatcaggaggagctcagt 
               
               
                   
                   
                 aaatgctggttgaatgaatgaatgaat 
               
               
                   
               
               
                 329 
                 AH25-1 
                 tgacctggtcactttctatgtggcttcctgtattccctttgttgtctaatgtcagaaactataactatcta 
               
               
                   
                   
                   [g/a] ttcacactaggttctctataaattatttgctgaacaaaatatttcttcttttgaaaataagagaaa 
               
               
                   
                   
                 catagagtttac 
               
               
                   
               
               
                 330 
                 MBS046-1 
                 ctgggagtggagagtggattgggaagcctggggaactgtggacctgtgggcaatcccttagc [t/   
               
               
                   
                   
                   c] tttctgagcctcagtttctccatctgtacaaaaggggcaatcataccNatttcacagtcaggtga 
               
               
                   
                   
                 actgtgcag 
               
               
                   
               
               
                 331 
                 MBS047-1 
                 gccgtctacacatgcatatctgaaaggaatgaccctcctaggcagaggaggagaaagaatcat 
               
               
                   
                   
                 ttaaatgtctttacagtaatgctc [g/t] aaaatttttggctggccagcatggctgctttattacatctctc 
               
               
                   
                   
                 caaagagcacaNtacctgccatcaagtgcagatacgcat 
               
               
                   
               
               
                 332 
                 MBS018-1 
                 atgcttgttctcgcttgtgcagaaaacattgttccagattcaatcgactgggttcatgtcccctcacat 
               
               
                   
                   
                 agtttttaaggttatttatttaaa [g/t] gtctaatgtattttattgtaacagacattgttttgccaacattgc 
               
               
                   
                   
                 ctatttca 
               
               
                   
               
               
                 333 
                 MBS020-1 
                 gcaggccttcgagtccatgctgcgctaNatctactatggcgaggtcaacatgccgcccgaggac 
               
               
                   
                   
                 tccctgcatcctagcctcagggcttcc [g/a] gccacgcccggcagccttggaccccgccagcc 
               
               
                   
                   
                 aggggctgggcNgtttcccaaggattcNtggtggccccgtctcccctgagctctct 
               
               
                   
               
               
                 334 
                 MBS033-1 
                 gagcgcctgatggaagagggtctgggggcctgggttcttgggtccagaggatgaagtggcagg 
               
               
                   
                   
                 ggacgccgattctt [c/t] ggtcccaagagaggaagggcctgagtcttgctgaaggaggagact 
               
               
                   
                   
                 gggacctcaatttctgggtcccaaaagagaaatgttg 
               
               
                   
               
               
                 335 
                 MBS021-1 
                 tgtcttccactttctactgcgattNgtcacttcttcatccatgggagggagaggagagctcttctcag 
               
               
                   
                   
                 attgcctgatttcc [t/g] attcctttcatcctcagccggctcttcccagacaaggagagcatgcttga 
               
               
                   
                   
                 tgcggctttcaccttggcagctgagatttccagcaag 
               
               
                   
               
               
                 336 
                 AH13-4 
                 ctttctgacttgaaaccatttNgaggagacaggggggatctttaagaggtaacttcagtcttcgag 
               
               
                   
                   
                 gttagggtccccactttgtagaggggatgagaa [a/g] ttggttttgcagctgatgggtccagtgt 
               
               
                   
               
               
                 337 
                 MBS034-1 
                 cctactcccagtccaagcagtttttactggactcaggtgagacccagagctgagccctcagctcc 
               
               
                   
                   
                 cagctagtctggaccagcttcgagctgattgccacc [t/c] cctgctccacctcccccaggctactt 
               
               
                   
                   
                 ctctgacaacatcc 
               
               
                   
               
               
                 338 
                 MBS054-1 
                 tccgtgcctgccctcagcctgcccagcggggaagctctggtgggtgtggaatggtggtggcaga 
               
               
                   
                   
                 aagggt [c/a] ccgcgggtctcccattNgtcttcccctgagtccctgcccagccgggactgcctgg 
               
               
                   
                   
                 atctgagaggtgggacaaggaggtggcttNgccgcaggtcaccg 
               
               
                   
               
               
                 339 
                 MBS049-1 
                 ttctcatacaaaaagactttgttctgacagctgctcactgcagaggaaggtgaggggcagtagcc 
               
               
                   
                   
                 agcctaccctacctcaggcct [c/g] gacagggacccgtcctctccccaggagctgagcccagg 
               
               
                   
                   
                 ggtacccctccttcaccccaggatcctggaaatcaggaccacaagagctcatcag 
               
               
                   
               
               
                 340 
                 MBS025-1 
                 aagcagaggaggtccagagaagcctctgccccacccctcaggcctctgttggctccctggccc 
               
               
                   
                   
                 cactctcaaaatctgagacttgta [c/t] tcaacccttttctttcccaggaggttctagatctcctcaga 
               
               
                   
                   
                 ttccttcaacagcctcttcctgtaggaatgacccctcctgc 
               
               
                   
               
               
                 341 
                 MBS039-1 
                 ccccagcacagccccttgccaaggtatacatctatggatatctttggggtaatgaggaggaaaa 
               
               
                   
                   
                 ggtcttaaatggggtgg [a/t] tccagagtaaaaggcacctgtcttttctccacagaaaaaggggg 
               
               
                   
                   
                 taaagcggaaagcagatactaccacccctacacc 
               
               
                   
               
               
                 342 
                 MBS035-1 
                 ttgaacacttactatatacttggcattgttctaggttctgtgaatacagttNtgaacaaaacagagca 
               
               
                   
                   
                 aaaatccttgccttcatggg [a/g] tagggaatggggagacagacaatatacataataaataact 
               
               
                   
                   
                 aaatgtattttttgtgttaagaggtaataaatacagtggaa 
               
               
                   
               
               
                 343 
                 MBS028-1 
                 ttctacccacctaaatgagacattccttttatgtctagccctcactcccagtgtaaacctgttgtgtttcc 
               
               
                   
                   
                 ctgtattttcctgca [g/a] tgttgtcacagaggaagtggggtagtgtagttctgccttgatcacgctN 
               
               
                   
                   
                 tctctrtcttagctgttrtttaaaattttattgt 
               
               
                   
               
               
                 344 
                 MBS040-1 
                 aatcagttgacccttaaagacacttgtatgtatttcagaagttttctcatgccaagctaagagcagttt 
               
               
                   
                   
                 cacgtagtata [t/c] ttcagggtcaggaaggcNgcccataaagggcattgtttgtgatcctttgag 
               
               
                   
                   
                 gacgttgaggtgctgt 
               
               
                   
               
               
                 345 
                 MBS051-1 
                 tggagcagcaccccagctgccggtacgatgctctggagatctttgccgggtctggaacctcggg 
               
               
                   
                   
                 ccagcaactcggacgcttctg [t/c] gggaccttccgacccgcgcctgtagtcgcctccggcaac 
               
               
                   
                   
                 caggtgaccctgaggatgagatcggacgaggg 
               
               
                   
               
               
                 346 
                 MBS041-1 
                 gccttgtcagtgggtggggtgNggttgttgatggcagcggtggtgaagtgataacaaacagcca 
               
               
                   
                   
                 tctactgcctttttctcctggtaac [c/t] gttagcatttcctcttgtcctcactagacttccctgg 
               
               
                   
               
               
                 347 
                 421_10 
                 cttcttttaatttccaggtctcagtctgggagcccNgggaactgtgcccctgtttctgact [C/G] ttgt 
               
               
                   
                   
                 tgccttgagggttttggaggttgacatggtcagcaacccgaagaagcacctcagatggtctgtga 
               
               
                   
                   
                 gtttcagacacagtgataatcacagctggcccacagccagaggaggaggcatcctactaacaa 
               
               
                   
                   
                 gagcagtggatgctttggttatgcaaatgta 
               
               
                   
               
               
                 348 
                 423_24 
                 tgaagtgggagtagggtgccggcaaggcaaggcatagagcatgggaaaggaggcagggttt 
               
               
                   
                   
                 ggaaagcggaggcaggctggacagagacaagggttagtctctggaatgttgacatatgtggag 
               
               
                   
                   
                 cccaggggaggagggcctcatgtgccatgctaggc [G/A] atacaagattctcttcaagtctgg 
               
               
                   
                   
                 aggaactgctcaatgcagggggcttagagggccctacctgggtccttttctcatctctgccctgca 
               
               
                   
                   
                 agctNcccatgtgttctttaccccaaggtgtcggccatcacccatcccatggctatcaatctttcttcc 
               
               
                   
                   
                 tggctttgag 
               
               
                   
               
               
                 349 
                 425_2 
                 gagagcaccctctcatccctccttcagcttgagcagaccatcatcttccagtctctccagggcaag 
               
               
                   
                   
                 acaatctgggaattaccctttacaagagaaaagcttaaaatattatgaaacttaaaataattagtg 
               
               
                   
                   
                 acactttctaaaaatgtaataaatctgcaaagactattttaactctagagagattctttaatttacaatt 
               
               
                   
                   
                 tgttgagcttcataatcctattagaagtctgcccacaaaatactcaaaaatcccattcaaagcaga 
               
               
                   
                   
                 acagagaactgttcaacatctttccaacaggacagaagtcaacctgggaacagctgttctctctg 
               
               
                   
                   
                 cggctcaggtagtga [A/G] atgcacaggacctgtcctgggtactgtccctgtgagtattcaagct 
               
               
                   
                   
                 ggactcattagctgcagttactttgctgccagtcatgattccatacatacacacacacacatgcac 
               
               
                   
                   
                 gcacatgcaca 
               
               
                   
               
               
                 350 
                 431_a2 
                 TatctgtgcactcatcatgttcataNaaggattatttataatagacaaaattgtagaagaaaccca 
               
               
                   
                   
                 aaatgtccattgacgtttggaataaaaaatgcatgattggattcacaggtgaatgaataaatgaa 
               
               
                   
                   
                 aagtagtttatacatatgatggagtattattagccttaaaaaaaatctgacacgtgtgacaacatgg 
               
               
                   
                   
                 atgaatcttgaggacattatattaagtgaa [G/A] taagccaatcgtaaaagaaaaatgctatatg 
               
               
                   
                   
                 gtttcacttatatgaggtatccagaacagtcaaactcatagaaacagaaagtataatggcagttg 
               
               
                   
                   
                 ccaggggctggtggggaggtaaagtgaggagttaatgggactaaaatttcagtttttcaagataa 
               
               
                   
                   
                 aaaagttctgaagattagatgcacaaaatccgagtgcacttaacactgctgaatattcacttaaac 
               
               
                   
                   
                 ggtaataaagtttatgccatgtatcctttgcg 
               
               
                   
               
               
                 351 
                 487_67 
                 aaaattgtgagtaaacataaaaggcaaaaagttatgacactgaaagatgagccacccaagtc 
               
               
                   
                   
                 agaaggtgtccagtatgctgctggggaagagtggaagacaactctaatgtggctgggccaaag 
               
               
                   
                   
                 cagaaataacattcagttgtggatgt [G/A] tctggtggtgaaagtaaaatctgatgctataagca 
               
               
                   
                   
                 aaatgaactgagaactacctctactttagaactacctctagaaacatctacctgtttcattgacaact 
               
               
                   
                   
                 ctaaagactttgacaatgtggatcatgccaagctgtgaaaaactcttaaagagatgggaatacc 
               
               
                   
                   
                 agNccatcttacctgtctcctgagaaacctgtatgcaagacaagttgcaacagttgtatagaaca 
               
               
                   
                   
                 actgtatagaacaactgactggttcagaattgag 
               
               
                   
               
               
                 352 
                 448_67 
                 ttctatagacatagacttagaagagaggacatttgtttccctcctaggttcttaaaggaaaatagctt 
               
               
                   
                   
                 tcaaaa [T/C] ttaatttttattatgtttttgcaaacttgctaaacctagagaaaagcaatgttgttagg 
               
               
                   
                   
                 gggaggggaagtaagtttacataatacttataaatatttccttgatatctataaatatttgctca 
               
               
                   
               
               
                 353 
                 16_2 
                 caagctttaagagccactctctgccccctcataatctggtctcccccaccacccagacctgtctcc 
               
               
                   
                   
                 gccgggccgctcagttcccctcctcctacagactcactgaac [G/A] tgctgcctgactccctggt 
               
               
                   
                   
                 gtttccccccaccccccacagtactgtgcagccctggactccctgatcagcatctccaactgcagt 
               
               
                   
                   
                 gtcatccaaaggaccaagaggatgctgaatgcactctgtcctcacaagccctcagctaaggtaa 
               
               
                   
                   
                 ggcccccctgtcctttgacctggcacccacttctgccagccctgggctcaccctggga 
               
               
                   
               
               
                 354 
                 417_16 
                 actttggggtNaaacttccagaataaatatttgcaggggaagggtcactcagcccagcccctcc 
               
               
                   
                   
                 actgcctgggcatctgcattcttggaaggaaggaggtgatgtagggaggagggagagtgagga 
               
               
                   
                   
                 tgccacgtggagccNagctgcatttcac [G/A] tcacagttgatgtcaccacttggcagatgtccc 
               
               
                   
                   
                 agggcatggggggaatggtagactgtcaaagcaggtgtgaaacactgaactaaaacagacat 
               
               
                   
                   
                 ttagcaaatcaaacaaggagaaagtggttaagaNccagctgaagacactggcaaggcaagtt 
               
               
                   
                   
                 ctgatgctggc 
               
               
                   
               
               
                 355 
                 486_67 
                 ttactattatgcatttctgatcaccgacctaaactggggtttcaactcttattagcatttttagtatgatctt 
               
               
                   
                   
                 acttgtttctgttgNgacctcattctcctacttactgtaaaactaNNtctatcttctctattg [C/T] tca 
               
               
                   
                   
                 gagtcttcattgaactaaactggtaaacaaaccacacagatgtgaaaataaatttctaatctatta 
               
               
                   
                   
                 agttaaattttgctatataaaaacacttgttaaaatttaaaaaatagaactaccattt 
               
               
                   
               
               
                 356 
                 436_C10 
                 aactgtagaatctatatgcttagcttgtttagtggttcacagggtaatcaatagtaaaagtgggttaa 
               
               
                   
                   
                 attcaatgtaatccagacaactgcaaggtatgtatatatattatacata [C/T] ataatttaaatgttg 
               
               
                   
                   
                 atcataaaactcacaaagaaatttatattaaaaaatagaatgctatcatgcattattttatttagacct 
               
               
                   
                   
                 tttagtcaccaagaggaggtaatttttccttagtctgtaaaattcaggaaattgaccatttaaag 
               
               
                   
               
               
                 357 
                 454_g11 
                 cagaNagttataaatgctaatttaagagtataatgccatccaa [C/G] tgcctaattgagaacaat 
               
               
                   
                   
                 gtaagaataaatttgaggaaaataaatacttgcaaactgtgctaattatatatttgatcagaaaatt 
               
               
                   
                   
                 cacatagaaaatattttaaattggtgtttttccacagtggatg 
               
               
                   
               
               
                 358 
                 013.SP3 
                 gattggagtgttgcatgtataagccagggaacaccagcaattgtcagcaacaccagaagctaa 
               
               
                   
                   
                 gagaaagacatggaacagattct [T/C] tcctggacccttcagggaaagcatgatcttgttaaca 
               
               
                   
                   
                 ttttgaactaatagcNtccagaactgtgagaaactgggagtttctattgtttaaagtgaaagtgaag 
               
               
                   
                   
                 ttgctcagtcgtatccaactcttt 
               
               
                   
               
               
                 359 
                 018.SP6 
                 accacgctcaaagctcaggtcctgagaatatgaccctccccaccaggccccagattctgcagc 
               
               
                   
                   
                 caatgtgaccttgattcttctgggaactactcaaaggcccaaggctc [C/T] tctacccaagggtt 
               
               
                   
                   
                 gctgaaaatccatcaagagcccagcaagagggagaggcagggtgtgggtctgagctccaac 
               
               
                   
                   
                 ccacaggcaacaagtcttttNgggggagagaggg 
               
               
                   
               
               
                 360 
                 BLAD 
                 Ctatgtcagaacgtgtgcttgcctgaaatggaatctgaataggcatcctgcatcatatccaccagc 
               
               
                   
                   
                 ataagagaatggggagagtcctgaggttctgaggcctgacaagatgccataagtgcccatgaa 
               
               
                   
                   
                 ccccccccacccccagaccagatagtacaccctgactatctcccaaatcctggcaggtcaggc 
               
               
                   
                   
                 agttgcgttcaacgtgaccttccggagggccaagggctaccccatcg [a/g] cctgtactacctga 
               
               
                   
                   
                 tggacctctcctactccatggtggatgacctcgtcaacgtcaagaagctggggggtgacctgctc 
               
               
                   
                   
                 cgggccctcaatggcatcaccgagtcgggccgcattggtga 
               
               
                   
               
               
                 361 
                 DUMPS 
                 GTGCAAATGGCTGAAGAACATTCTGAATTTGTGATTGGTTTTATTT 
               
               
                   
                   
                 CTGGCTCC [C/t] GAGTAAGCATGAAACCAGAATTTCTTCACTTGAC 
               
               
                   
                   
                 TCCAGGAGTTCAGTTAGAAGCAGGAGGT 
               
               
                   
               
               
                 362 
                 CHONDR 
                 CTTATTAATTAAGGACAAACGTCATTTCACTGGACTGGGTAGGGG 
               
               
                   
                   
                 TTGGAAGTCCAAGGACTAGCAAGATTTGTGCACAAGCCTGGCCC 
               
               
                   
                   
                 ACCTCATGGGCCCCTGGGTCCCCTGAAGAAGGGCCAGCCTGGG 
               
               
                   
                   
                 GTGGGTCACTGGTACAGGAGCCCCCAGCCCTCACCANACATGTC 
               
               
                   
                   
                 CTCTTTAGGTGTTTCAGCGGCGCCCTCTCCAGAAGAAGAGGAGG 
               
               
                   
                   
                 GTAGTGCACCCACAGCAGGCCCTGACGTGGAGGAGTGGATGGT 
               
               
                   
                   
                 GACACAAGTGGGGCCTGGCGTGGCTGCTGTCCCCATCGGGGAG 
               
               
                   
                   
                 GAGACGACTGCAATCCCAGGCTTCACCGTTGAGCCAGAAAACAA 
               
               
                   
                   
                 GACGGAATGGGAACTTGCCTACACCCCAGCGGGCACTTTGCCAC 
               
               
                   
                   
                 TAC [*/GGCA] CAGGTCCGTCCGGGCTCTCCTGCATGTCCTGCTGC 
               
               
                   
                   
                 CTCCCTGGGCCAGGGTGTGGCCTGGAAGGGGGGAGGAGGAAGT 
               
               
                   
                   
                 GTTCTCTCCCTGGGACCCGTGATCTGTTCCCCAGCCCTGACCCC 
               
               
                   
                   
                 CAGCCCTGATTTTATTTAAGTGTGCTGCCATGGGTAACTTCAGCC 
               
               
                   
                   
                 ACCTCAGCAGGCATCCAGGACCAATCCTA 
               
               
                   
               
               
                 363 
                 PROTO 
                 cggtgtctgcgcttctgaccgtctgtccttcccgtctgtcctgcaggccctggccgacctggtccact 
               
               
                   
                   
                 cacacctccagtccaaggagcgctgctccacacggctgactctgagctgtccgctctgcgtgaa 
               
               
                   
                   
                 ccccacctgcagggagaccaaatccttcttcaccagccagcagctgt [g/t] accctggcggcac 
               
               
                   
                   
                 gccgctgggaggtgcgcgtgcccgcctcccgacacctccgaggaggaggagggcgcatccg 
               
               
                   
                   
                 gccgttagggaggaggttacatccg 
               
               
                   
               
               
                 364 
                 HYPOTR 
                 Tccctgcagctctgagtcctggaaaataaggctcagttgatgcttggcaaaaggctcagactga 
               
               
                   
                   
                 gcctggcttggctcatacagggagcaaaagctcagtgccattggctgcctagatgaagaggaa 
               
               
                   
                   
                 agcaagtagacagagcatgccctctgactggcctgtcctattttgcaggtgctgctgataaagctg 
               
               
                   
                   
                 gaact [c/t] gagaaaaccaggttggctgggaattgctgtcttgctgggaaggagggaaggcca 
               
               
                   
                   
                 caggcctgagccacccttgagtttgctccctgctaagttttctgaggctttcttttgtgaggagaccct 
               
               
                   
                   
                 ggaggttc 
               
               
                   
               
               
                 365 
                 SYNDAC 
                 Ctgttaataaccaagacgatcagagccaccggagagccacgttcacatctgaagctcttagtcc 
               
               
                   
                   
                 ttttcggccccctgttgttatatccctccttcctgccccactgtccctgggagtcagggagccctgaat 
               
               
                   
                   
                 ctcctccatttggagaagcgtcaaactgggagacttgattctgccccaggccagatgttcatttgctt 
               
               
                   
                   
                 tgctcatagggaccaacgcttgtggcgtgaacaa [cg/at] gcggatgcactcacctctgctttgc 
               
               
                   
                   
                 cagaacctcggactttgtgtgtgcctgtcctgatgagcccgacggccggccctgctccctcggtga 
               
               
                   
                   
                 gttggactgacgggcccccctgcaacagcgga 
               
               
                   
               
               
                 366 
                 CITRUL 
                 TGGTCACCCGTCACGATGTCCGGCAAAGGCTCCGTGGTTCTGGC 
               
               
                   
                   
                 CTACAGTGGGGGCCTGGACACCTCCTGCATCCTCGTGTGGCTGA 
               
               
                   
                   
                 AGGAGCAAGGCTATGACGTCATTGCCTACCTGGCCAACATCGGC 
               
               
                   
                   
                 CAGAAAGAAGACTTTGAGGAAGCCAGGAAGAAGGCGCTGAAGCT 
               
               
                   
                   
                 TGGGGCCAAAAAGGTGTTCATTGAGGACATCAGCAAGGAGTTTG 
               
               
                   
                   
                 TGGAGGAGTTCATCTGGCCGGCCATCCAGTCCAGCGCACTGTAC 
               
               
                   
                   
                 GAGGAC [C/t] GATACCTCCTGGGCACCTCTCTCGCCAGGCCCTGC 
               
               
                   
                   
                 ATCGCCCGCAAGCAGGTGGAGATCGCCCAGCGAGAAGGAGCCA 
               
               
                   
                   
                 AGTATGTGTCT 
               
               
                   
               
               
                 367 
                 CVM 
                 tttttaaaattatagattgtaaaggcaatatcactatgggaaaaaaaaatgattctaaggttttttcaa 
               
               
                   
                   
                 aagctctcctctgtaatccccaggaatggaaatggttgcatttttaccttaaggtctaagagtgggct 
               
               
                   
                   
                 ctaaacatgtattttgtaaaatattataggaattaaacttgtgttgtttctttttgttcagtggccctcagat 
               
               
                   
                   
                 tctcaagagcttaattctaaggaactttcagctggctcacaatttgtaggtctcatggca [g/t] ttctc 
               
               
                   
                   
                 acagcatgtttttccagtggctttgctggggtttactttgagaaaatcttaaaagaaaccaaacaatc 
               
               
                   
                   
                 agtgtggataagaaacattcaacttggtaagttttaaatgttttctaacattacttttaaagtgattatat 
               
               
                   
                   
                 tgttatatttaaagatttctatgtatctttaattaaataaaccttataaaaactgcttgttgttg 
               
               
                   
               
               
                 368 
                 E+ 
                 GGGGAGCCATGAGTTGAGCAGGACCCTGAGAGCAAGCACCCCT 
               
               
                   
                   
                 TCCTGCTCCCTGCGGGACGATGCCTGCACTTGGCTCCCAGAGGC 
               
               
                   
                   
                 GGCTGCTGGGTTCCCTTAACTGCACGCCCCCAGCCACCCTCCCC 
               
               
                   
                   
                 TTCACCCTGGCCCCCAACCGGACGGGGCCCCAGTGCCTGGAGG 
               
               
                   
                   
                 TGTCCATCCCTGANGGGCTCTTTCTCAGCCTGGGGCTGGTGAGT 
               
               
                   
                   
                 CTCGTGGAGAACGTGCTGGTAGTGGCTGCCATTGCCAAGAACCG 
               
               
                   
                   
                 CAACCTGCACTCCCCCATGTACTACTTTATCTGCTGCCTGGCTGT 
               
               
                   
                   
                 GTCTGACTTGCTGGTGAGCGTCAGCAACGTGCTGGAGANGGCAG 
               
               
                   
                   
                 TCATGC [T/C] GCTGCTGGAGGCCNGTGTCCTGGCCACCCAGGCG 
               
               
                   
                   
                 GCCGTGGNGCAGCAGCTGGACAATGTCATCGACGTGCTCATCTG 
               
               
                   
                   
                 CGGATCCATGGTGTCCAGCCTCTGCTTCCTGGGTGCCATTGNTG 
               
               
                   
                   
                 TGGACCGCTACATCTCCATCTTCTACGCCCTGCGGTACCACNGT 
               
               
                   
                   
                 GTTGTGACACTGCCCCGAGCGTGGAGGATCATTGCGGCCATCTG 
               
               
                   
                   
                 GGTGGCCAGCATCCTCACCAGCCTGCTCTTCATCACCTACTACAA 
               
               
                   
                   
                 CCACAAGGTCATCCTGCTGTGCCTCGTTGGCCTCTTCATAG 
               
               
                   
               
               
                 369 
                 RED 
                 GGGGAGCCATGAGTTGAGCAGGACCCTGAGAGCAAGCACCCCT 
               
               
                   
                   
                 TCCTGCTCCCTGCGGGACGATGCCTGCACTTGGCTCCCAGAGGC 
               
               
                   
                   
                 GGCTGCTGGGTTCCCTTAACTGCACGCCCCCAGCCACCCTCCCC 
               
               
                   
                   
                 TTCACCCTGGCCCCCAACCGGACGGGGCCCCAGTGCCTGGAGG 
               
               
                   
                   
                 TGTCCATCCCTGANGGGCTCTTTCTCAGCCTGGGGCTGGTGAGT 
               
               
                   
                   
                 CTCGTGGAGAACGTGCTGGTAGTGGCTGCCATTGCCAAGAACCG 
               
               
                   
                   
                 CAACCTGCACTCCCCCATGTACTACTTTATCTGCTGCCTGGCTGT 
               
               
                   
                   
                 GTCTGACTTGCTGGTGAGCGTCAGCAACGTGCTGGAGANGGCAG 
               
               
                   
                   
                 TCATGCNGCTGCTGGAGGCC [G/*] GTGTCCTGGCCACCCAGGCG 
               
               
                   
                   
                 GCCGTGGNGCAGCAGCTGGACAATGTCATCGACGTGCTCATCTG 
               
               
                   
                   
                 CGGATCCATGGTGTCCAGCCTCTGCTTCCTGGGTGCCATTGNTG 
               
               
                   
                   
                 TGGACCGCTACATCTCCATCTTCTACGCCCTGCGGTACCACNGT 
               
               
                   
                   
                 GTTGTGACACTGCCCCGAGCGTGGAGGATCATTGCGGCCATCTG 
               
               
                   
                   
                 GGTGGCCAGCATCCTCACCAGCCTGCTCTTCATCACCTACTACAA 
               
               
                   
                   
                 CCACAAGGTCATCCTGCTGTGCCTCGTTGGCCTCTTCATAG 
               
               
                   
               
               
                 370 
                 DUN 
                 TATAAAATATGAAAGAACTTTTATTGTTACCCTTAAACATTTTAAGT 
               
               
                   
                   
                 CACCTTCAGAACATAATAATATATTAATACAAACTGATTATGTCTAT 
               
               
                   
                   
                 TAACAAGGTGTCTTTGACATATTTTAGATATATCCACATATCCACT 
               
               
                   
                   
                 GGAAAATGCCCCTATTGGA [C/T] ATAACAGACAATACAATATGGTA 
               
               
                   
                   
                 CCATTTTGGCCTCCAGTTACCAACATAGAAATGTTTGTTACTGCTC 
               
               
                   
                   
                 CAGACAACCTGGGCTATACTTANGAAGTTCAATGGCCAAGTGAGT 
               
               
                   
                   
                 ACTGAAAATGTATTTTTACTGTGGAAATTTCCAAAATCAAACTTGT 
               
               
                   
                   
                 TACCTTTAAAGTAATCTCAGTTTTCTGAGATAAAGTAACC 
               
               
                   
               
               
                 371 
                 ALBINO 
                 gcagatcgtctgcagcagactggaggagtacaacagtcgccaggctttatgcaacgggacgtc 
               
               
                   
                   
                 tgagagaccattactgcgcaatcctggaaaccacgacaaagccaggacccc [*/c] gaggctc 
               
               
                   
                   
                 ccctcctcggctgatgtggagttttgcctgagtttgacccagtatgaatcaggttccatggataaag 
               
               
                   
                   
                 ctgccaatttcagctttaga 
               
               
                   
               
               
                 372 
                 ROAN 
                 gaaggcctcaaattccattgaagattccagcctacaatgggcag [c/a] cgtagcattgccagca 
               
               
                   
                   
                 ttcttttctcttgtgatcgggtttgcttttggggccttttactggaag 
               
               
                   
               
               
                 373 
                 Ucalp 
                 agcatcctcggggcgtctgagctggccctcataagataacccctgggactggggtctctggactt 
               
               
                   
                   
                 gcccttgtggaggcctcctgacctgggccagggaaggacaggccccagggatagaggctggg 
               
               
                   
                   
                 caggtcagtggccgccagcccctggcagtgccgttttcctacagctcctcggagtggaacg [g/c]   
               
               
                   
                   
                 cgtggacccttacatgcgggagcagctccgggtcaagatggaggatggggagttctggtgagc 
               
               
                   
                   
                 agccccctcctcagtctgagtgggcaccccagctcccaaccccacccccctgaaaaccagctg 
               
               
                   
                   
                 tgccatgtctcttgatgcctcgactgggcatcctggttcactctc 
               
               
                   
               
               
                 374 
                 CALPA 
                 attttgaactctcatctttcaacacttaagtcctacctagaatggcagttatttgtttttctgttaaaacgg 
               
               
                   
                   
                 cacctctgtgtggcatcagcaggtattgcaatttgcttgtgtgattcttgctgaatttggaaggaagg 
               
               
                   
                   
                 aattgcattgtttcaaatttt [g/c] tacccaaagtgaaatttgtcacatgtaaatcatactaatttaaat 
               
               
                   
                   
                 tctcacaattgactacataaaacacaagtgttatgaattgctttctactcctcagagaaaagtagca 
               
               
                   
                   
                 atatgtgtcatattattaaccccatg 
               
               
                   
               
               
                 375 
                 MYOS 
                 ggaaaatgtggaaaaagaggggctgtgtaatgcatgtttgtggagggaaaacactacatcctca 
               
               
                   
                   
                 agactagaagccataaaaatccaaatcctcagtaaacttcgcctggaaacagctcctaacatca 
               
               
                   
                   
                 gcaaagatgctatcagacaacttttgcccaaggctcctccactcctggaactgattgatcagtt [c/   
               
               
                   
                   
                   a] gatgtccagagagatgccagcagtgacggctccttggaagacgatgactaccacgccagg 
               
               
                   
                   
                 acggaaacggtcattaccatgcccacggagtgtgagtagtcctgctggtgcagagcaacgactc 
               
               
                   
                   
                 tgctgactgctgttctagtgttcatgagaaaccgatctatttt 
               
               
                   
               
               
                 376 
                 ABCG2 
                 agtattcacgagactgtcagggacttaaagaggctatttgctagacggcaccagatctgattcttg 
               
               
                   
                   
                 gtatttgttttttgtagatattttcagggctgttggtaaatctcaaaaccgtcgtgccttggttgtcatggc 
               
               
                   
                   
                 ttcaatacttgagcattcctcgatacggct [a/c] tgcggtatgttctccttatctgtcaccgtgctggtt 
               
               
                   
                   
                 cattgtccccatgctggaaacagccagaataaagcctctcatatccttggccatgagctgtgcaa 
               
               
                   
                   
                 gttttaggacaatgaaggagagtttcctattaagccttgggtcaagttgataatcacctgggatttct 
               
               
                   
                   
                 ctagtcaccttgttgtctgagg 
               
               
                   
               
               
                 377 
                 Kcasein 
                 GCCCAAATTCTTCAATGGCAAGTTTTGTCAAATACTGTGCCTGCC 
               
               
                   
                   
                 AAGTCCTGCCAAGCCCAGCCAACTACCATGGCACGTCACCCACA 
               
               
                   
                   
                 CCCACATTTATCATTTATGGCCATTCCACCAAAGAAAAATCAGGAT 
               
               
                   
                   
                 AAAACAGAAATCCCTACCATCAATACCATTGCTAGTGGTGAGCCT 
               
               
                   
                   
                 ACAAGTACACCTACCA [c/T] CGAAGCAGTAGAGAGCACTGTAGCT 
               
               
                   
                   
                 ACTCTAGAAG [a/C] TTCTCCAGAAGTTATTGAGAGCCCACCTGAGA 
               
               
                   
                   
                 TCAACACAGTCCAAGTTACTTCAACTGCGGTCTAAATACTCTAAG 
               
               
                   
                   
                 GAGACATCAAAGAAGACAACGCAGGTAAATAAGCAAAATGAATAA 
               
               
                   
                   
                 CAGC 
               
               
                   
               
               
                 378 
                 zfxy1 
                 AGTAGAGGCAGAAATCGTCACTGATCCTCTGACAGCCGA [t/c] GTA 
               
               
                   
                   
                 GTGTCAGAAGAAGTATTGGTAGCAGATTGTGCCTCAGAAGCAGT 
               
               
                   
                   
                 CATAGATGCCAACG 
               
               
                   
               
               
                 379 
                 zfxy2 
                 atgtggctgcccacaagggtaaaaaaatgcaccagtgtagacattgtgactttaagattgcagat 
               
               
                   
                   
                 cc [t/a] tttgttctaagtcgccatattctctcagttca 
               
               
                   
               
               
                 380 
                 GHR 
                 Caccaagtgccgttcacctgaactggagactttctcatgtcactggacagatggggctaatcaca 
               
               
                   
                   
                 gtttacagagcccaggatctgtacagatgttctatatcagaaggtatgggcttcatgcttttctgatttc 
               
               
                   
                   
                 t [c/g] tccatgaattttctgatgaaaatccattgagtgtcatgcagt [a/g] gtgggaatggaaata 
               
               
                   
                   
                 atcttctttggtgatctaaatgcattcacccattcattcatttaaatatattagttaagcccttactatatgt 
               
               
                   
                   
                 tggg 
               
               
                   
               
               
                 381 
                 Bcasein 
                 GATGAACTCCAGGATAAAATCCACCCCTTTGCCCAGACACAGTCT 
               
               
                   
                   
                 CTAGTCTATCCCTTCCCTGGGCCCATCC [C/A] TAACAGCCTCCCA 
               
               
                   
                   
                 CAAAACATCCCTCCTCTTACTCAAACCCCTGTGGTGGTGCCGCCT 
               
               
                   
                   
                 TTCCTTCAGCCTGAAGTAATGGGAGTCTCCAAAGTGAAGGAGGC 
               
               
                   
                   
                 TATGG 
               
               
                   
               
             
          
         
       
     
       Methods of Simultaneously Identifying a Plurality of Polymorphisms for the Determination of at Least Two Characteristics in an Animal 
       [0084]    The present invention provides for methods of simultaneously and efficiently identifying a plurality of nucleotide polymorphisms that correlate with at least two characteristics, wherein the characteristics include parentage, identity, sex, genotype and/or phenotype. Thus, profiles for individual animals or groups of animals may be formed for future use or to research animal history. 
         [0085]    In one method, the presence of a plurality of nucleotide polymorphisms are detected by performing PCR assays using an assay plate or panel, wherein each assay plate contains over 3.000 assays. e.g., 3072. An example of such a plate or panel is OpenArray™. In certain embodiments, four plates each containing over 3,000 assays each for a total of over 12,000 assays can be performed simultaneously. In other embodiments, multiple machines, each having four assay plates, can simultaneously perform between about 24,000 assays to several hundreds of thousands of assays. Each assay on the plate or panel is capable of detecting the presence of a polymorphism contained within a nucleotide marker sequence as provided in Tables 1-11. In particular, each assay is capable of discriminating alleles of a polymorphic sequence by detection of either allele 1, allele 2, or allele 1 and allele 2 at the polymorphic site in a nucleic acid sample. 
         [0086]    Each individual assay, according to the method above, contains a nucleic acid sample, sequence-specific forward and reverse primers to amplify the polymorphic sequence of interest, two modified oligonucleotide probes (e.g., TaqMan® probes) and a DNA polymerase. One oligonucleotide probe matches the Allele 1 sequence; the other oligonucleotide matches the Allele 2 sequence. Each modified oligonucleotide probe contains a reporter dye at the 5′ end of the probe (e.g., a VIC® dye, or a FAM™ dye). A nonfluorescent quencher is attached at the 3′ end of the probe. Oligonucleotide probes of the present invention are 25 to 35 nucleotides in length, but more preferably 30 nucleotides in length and perfectly complementary to a region within the nucleotide marker sequence referred to as the invariant region. The invariant region contains no further polymorphisms, other than the polymorphism utilized to discriminate allele 1 from allele 2. 
         [0087]    In the present invention, according to the method above, the forward and reverse primers hybridize to a sequence of DNA within the nucleic acid sample that is either upstream or downstream of a sequence corresponding to the invariant region within the nucleotide marker. The sequence is then amplified by PCR. During the PCR reaction, each oligonucleotide probe anneals specifically to a region spanning the invariant sequence of the nucleotide marker. The DNA polymerase contained within the assay mix can cleave the oligonucleotide probe only if it specifically hybridizes to a PCR-amplified sequence present within the sample. Cleavage separates the reporter dye from the quencher dye, increasing fluorescence by the reporter. Thus, the fluorescence signal(s) generated by PCR amplification indicates the presence of a specific polymorphic allele within the nucleic acid sample. 
         [0088]    Oligonucleotide probes used in allele discrimination are linear fluorescently-labeled probes used to monitor PCR product formation either during or after the amplification process. As the DNA polymerase extends the upstream primers and encounters the downstream probe, the 5′ to 3′ nuclease activity of the polymerase cleaves the probe. Following cleavage, the reporter fluorophore is released into the reaction solution and fluorescence is detected. 
         [0089]    More specifically, an oligonucleotide probe, containing a fluorescent dye at the 5′ end, that matches the Allele 1 sequence will generate a fluorescence signal at the wavelength of that fluorescent reporter dye only if the Allele 1 sequence is present in the nucleic acid sample. Similarly, a second oligonucleotide probe, containing a fluorescent dye at the 5′ end, that matches the Allele 2 sequence will generate a fluorescence signal at the wavelength of that fluorescent reporter dye only if the Allele 2 sequence is present in the nucleic acid sample. In this way the presence of either Allele 1, Allele 2, or both Allele 1 and Allele 2 of a nucleotide marker sequence of the present invention can be identified from an isolated nucleic acid sample in the assay described above using two different fluorescent dyes for each probe. Fluorescent dyes can include VIC®, FAM™, and other dyes known those of ordinary skill in the art. 
         [0090]    In certain embodiments, a polymorphism of the present invention can be identified in part, by its position within a 30 nucleotide invariant region using the polymerase chain reaction in combination with oligonucleotide probes. This position can be, for example, the position within brackets and in bold, as shown in Tables 2, 4 and 6 above. 
         [0091]    The present invention provides for a method as described above, wherein a single plate comprises 64 assays for identification of the polymorphic sites within the nucleotide markers according to Table 2 and/or 64 assays for identification of the polymorphic sites within the nucleotide markers according to Table 4 and/or 128 assays for the identification of the polymorphic sites within the nucleotide markers according to Table 6. In other embodiments, nucleotide markers according to Table 7 or 9 and 10 are used to detect polymorphic sites within the nucleotide markers according to Tables 8 and 11 respectively. A single plate may be any available or offered to those in genetic screening arts and is thus nonlimiting. 
         [0092]    PCR reactions are performed using assay plates according to the method above by simultaneously thermal cycling using a commercial flat-block thermal cycler. The fluorescence output is subsequently read using a computer-based imaging system. Each plate is capable of performing over 3000 assays simultaneously. One, two or three plates performing over 3000 assays can be performed simultaneously. 
         [0093]    In this way, high-throughput cost-efficient analysis of over 3000, 6000 or 12,000 (e.g., 3072, 6344, 9216 or 12,288) polymorphic sites can be assayed simultaneously. The present invention therefore provides a rapid and powerful method to simultaneously determine at least two characteristics, such as parentage, identity and/or phenotype in a single animal, in more than one animal and/or in more than one species of animal at a much lower cost than previous systems. 
         [0094]    A nucleic acid sample useful for practicing a method of the invention can be any isolated biological sample obtained from an animal, such as an equine, canine, feline, or human, that contains nucleic acid molecules, including portions of the gene sequences to be examined, or corresponding encoded polypeptides, depending on the particular method. As such, the sample can be a cell, tissue or organ sample, or can be a sample of a biological material such as blood, milk, semen, saliva, hair, tissue, and the like. A nucleic acid sample useful for practicing a method of the invention can be deoxyribonucleic (DNA) acid or ribonucleic acids (RNA). The nucleic acid sample generally is a deoxyribonucleic acid sample, particularly genomic DNA or an amplification product thereof. However, where heteronuclear ribonucleic acid, which includes unspliced mRNA precursor RNA molecules and non-coding regulatory molecules such as RNA, is available, a cDNA or amplification product thereof can be used. 
         [0095]    In another aspect of the invention, the identification of a plurality of polymorphisms can be performed where the oligonucleotide markers are attached to the assay plate itself, and polymorphisms are detected by hybridization of an isolated nucleic sample to the oligonucleotide marker itself. In such a method, a plurality of nucleotide marker sequences is utilized, wherein each of said nucleotide marker sequences comprises a polymorphism, and wherein said plurality of nucleotide marker sequences correlates with at least two characteristics selected from the group consisting of: (i) parentage; (ii) identity; (iii) genotype (iv) phenotype; and wherein each of said nucleotide marker sequences is complementary to a nucleotide sequence derived from one or more animals. 
         [0096]    In such a method, at least two characteristics of an animal are determined by: (a) contacting a nucleic acid sample with the composition comprising oligonucleotide markers; (b) hybridizing said nucleic sample to said plurality of nucleotide marker sequences in said composition; and (c) detecting oligonucleotide sequences within said nucleic sample that have hybridized to said plurality of nucleotide marker sequences, wherein each of said nucleotide marker sequences is complementary to an oligonucleotide sequence derived from one or more animals. 
         [0097]    In certain embodiments, the nucleic sample is detectable labeled, and the hybridization of the nucleic acid sample with the nucleotide marker sequence results in fluorescence. 
         [0098]    In certain other embodiments, the nucleotide marker sequences are attached to a substrate where the substrate can be, for example, a chip, wafer, slide, membrane, particle, bead, or any surface which would be compatible with the assay considered. 
         [0099]    As used herein, the terms “bead,” “microsphere,” “microparticle,” and “particle” are used interchangeably. Bead composition may include, but is not limited to, plastics, ceramics, glass, polystyrene, methylstyrene, acrylic polymers, paramagnetic materials, carbon graphite, titanium dioxide, latex or cross-linked dextrans such as sepharose, cellulose, nylon, cross-linked micelles and polytetrafluoroethylene. 
         [0100]    Beads may be associated with a physically or chemically distinguishable characteristic. For example, beads may be stained with sets of optically distinguishable tags, such as those containing one or more fluorophore or chromophore dyes distinguishable by excitation wavelength, emission wavelength, excited-state lifetime or emission intensity. Optically distinguishable dyes combined in certain molar ratios may be used to stain beads in accordance with methods known in the art. Combinatorial color codes for exterior and interior surfaces are disclosed in International Application No. PCT/US98/10719, incorporated herein by reference. Beads capable of being identified on the basis of a physically or chemically distinguishable characteristic are said to be “encoded.” 
         [0101]    The detection of the chemically or physically distinguishable characteristic of each set of beads and the identification of optical signatures on such beads generated in the course of a genetic or other test (such as diagnostic or prognostic test) using such beads may be performed by respectively recording a decoding image and an assay image of a set or array of such beads and comparing the two images. For example, in certain embodiments, a system with an imaging detector and computerized image capture and analysis apparatus may be used. The decoding image is obtained to determine the chemical and/or physical distinguishable characteristic that uniquely identifies the probe displayed on the bead surface. In this way, the identity of the probe on each particle in the array is provided by the distinguishable characteristic. The assay image of the array is obtained to detect an optical signature produced in the assay as elaborated herein below. 
         [0102]    In addition to being encoded, beads having specific oligonucleotide probes or primers may be spatially separated in a manner such that the bead location provides information about bead and hence about probe or primer identity. In one example, spatial encoding may be provided by placing beads in two or more spatially separate subarrays. 
         [0103]    In a preferred embodiment, beads can be arranged in a planar array on a substrate before decoding and analysis. Bead arrays may be prepared by the methods disclosed in PCT/US01/20179, incorporated herein by reference in its entirety. Bead arrays also may be formed using the methods described in U.S. Pat. No. 6,251,691, incorporated herein by reference in its entirety. For example, light-controlled electrokinetic forces may be used to assemble an array of beads in a process known as “LEAPS”, as described in U.S. Pat. No. 6,251,691. Alternatively, if paramagnetic beads are used, arrays may be formed on a substrate surface by applying a magnetic field perpendicular to the surface. Bead arrays also may be formed by mechanically depositing the beads into an array of restraining structures (e.g., recesses) at the surface of the substrate. In certain embodiments, the bead arrays may be immobilized after they are formed by using physical means, such as, for example, by embedding the beads in a gel to form a gel-particle film. 
         [0104]    A target that forms a hybridization complex with immobilized probes can be visualized by using detection methods previously described herein. For example, probes annealed to target strands can be elongated with labeled dNTPs, such that extension occurs when the probe perfectly matches the number of repeats in the target. Several other configurations for generating positive assay signals may be readily constructed. 
         [0105]    As described for sequence-specific probes in general, parallel interrogation repeated sequences may be immobilized on solid supports via a linker moiety, use of which is well known in the art. As a general rule, probes should be sufficiently long to avoid annealing to unrelated DNA target sequences. The length of the probe may be about 10 to 50 bases, more preferably about 15 to 25 bases, and even more preferably 18 to 20 bases. In a multiplexed assay, one or more solution-borne targets are then allowed to contact a multiplicity of immobilized probes under conditions permitting annealing and elongation reactions. 
         [0106]    The present invention offers advantages over the existing methods of analyzing polymorphisms in animals because of the combination of nucleotide marker sequences that can be simultaneously detected, and because of the efficient and cost-efficient method by which a large number of nucleotide markers can be assayed simultaneously. The present invention further offers advantages that at least two characteristics including parentage, identity and phenotype can be simultaneously determined in at least one, two, three or four and up to forty-eight different animals on one assay plate. 
         [0107]    The present system also offers the advantage of simultaneously detecting polymorphisms of the marker sequences as set forth in Tables 1-11. In this way, the present invention can simultaneously detect different kinds of polymorphisms including, but not limited to single nucleotide polymorphisms (SNPs), insertions and/or deletions and other mutations. 
         [0108]    In another aspect of the invention, a polymorphism within a nucleotide marker sequence can be detected based on the lack of incorporation of a specific nucleotide, for example a fluorescently-labeled or radiolabeled nucleotide. 
         [0109]    Additional methods known in the art can be utilized for determining the presence of a plurality of polymorphisms in a sample. 
         [0110]    For example, the identification can use microarray technology, which can be performed with PCR, for example using Affymetrix technologies and GenFlex Tag arrays (See e.g., Fan et al (2000) Genome Res. 10:853-860), or using a gene chip containing proprietary SNP oligonucleotides (See e.g., Chee et al (1996), Science 274:610-614; and Kennedy et al. (2003) Nature Biotech 21:1233-1237) or without PCR, or sequencing methods such as mass spectrometry, scanning electron microscopy, or methods in which a polynucleotide flows past a sorting device that can detect the sequence of the polynucleotide. The presence of a polymorphism can be identified using electrochemical detection devices such as the eSensor™ DNA detection system (Motorola, Inc., Yu, C. J. (2001) J. Am. Chem. Soc. 123:11155-11161). Other formats include melting curve analysis using fluorescently labeled hybridization probes, or intercalating dyes (Lohmann, S. (2000) Biochemica 4, 23-28, Herrmann, M. (2000) Clinical Chemistry 46: 425). 
         [0111]    An oligonucleotide ligation assay (Grossman, P. D. et al. (1994) Nucleic Acids Research 22:4527-4534) also can be used to identify a polymorphic site within a nucleotide marker sequence, wherein a pair of probes that selectively hybridize upstream and adjacent to and downstream and adjacent to the site of the polymorphism, and wherein one of the probes includes a terminal nucleotide complementary to the polymorphism. Where the terminal nucleotide of the probe is complementary to the SNP, selective hybridization includes the terminal nucleotide such that, in the presence of a ligase, the upstream and downstream oligonucleotides are ligated. As such, the presence or absence of a ligation product is indicative of the presence of the polymorphism. An example of this type of assay is the SNPlex System (Applied Biosystems, Foster City, Calif.). 
         [0112]    An oligonucleotide also can be useful as a primer, for example, for a primer extension reaction, wherein the product (or absence of a product) of the extension reaction is indicative of the polymorphism. In addition, a primer pair useful for amplifying a portion of the target polynucleotide including the polymorphic site can be useful, wherein the amplification product is examined to discriminate the alleles at a polymorphic site. Particularly useful methods include those that are readily adaptable to a high throughput format, to a multiplex format, or to both. The primer extension or amplification product can be detected directly or indirectly and/or can be sequenced using various methods known in the art. Amplification products which span a polymorphic site can be sequenced using traditional sequence methodologies (e.g., the “dideoxy-mediated chain termination method,” also known as the “Sanger Method” (Sanger, F., et al., J. Molec. Biol. 94:441 (1975); Prober et al. Science 238:336-340 (1987)) and the “chemical degradation method,” “also known as the “Maxam-Gilbert method” (Maxam, A. M. et al., Proc. Natl. Acad. Sci. (U.S.A.) 74:560 (1977)), both references herein incorporated by reference) to discriminate the alleles at the polymorphic site. 
         [0113]    Other techniques including fluorescence spectroscopy, capillary electrophoresis (CE), and high performance liquid chromatography (HPLC) can be used for detection. The presence of a nucleotide marker polymorphisms can also be determined using microchip electrophoresis such as described in Schmalzing et al.,  Nucl. Acid. Res.  28:e43 (2000). In addition, the presence of a nucleotide marker polymorphism can be determined using denaturing HPLC such as described in Nairz K et al (2002) Proc. Natl. Acad. Sci. (U.S.A.) 99:10575-80, and the Transgenomic WAVE™ System (Transgenomic, Inc. Omaha, Nebr.). 
         [0114]    Oliphant et al. report a method that utilizes BeadArray™ Technology that can be used in the methods of the present invention to determine the nucleotide occurrence of a SNP (supplement to Biotechniques, June 2002). Additionally, nucleotide occurrences for SNPs can be determined using a DNAMassARRAY system (SEQUENOM, San Diego, Calif.). This system combines proprietary SpectroChips™, microfluidics, nanodispensing, biochemistry, and MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). 
         [0115]    As another example, the presence of a nucleotide marker polymorphism in a sample can be determined using the SNP-ITT™ method (Beckman Coulter, Fullerton, Calif.). In general, SNP-ITT™ is a 3-step primer extension reaction. In the first step a target polynucleotide is isolated from a sample by hybridization to a capture primer, which provides a first level of specificity. In a second step the capture primer is extended from a terminating nucleotide triphosphate at the target polymorphic site, which provides a second level of specificity. In a third step, the extended nucleotide trisphosphate can be detected using a variety of known formats, including: direct fluorescence, indirect fluorescence, an indirect colorimetric assay, mass spectrometry, fluorescence polarization, etc. Reactions can be processed in 384 well format in an automated format using a SNPstream™ instrument (Beckman Coulter, Fullerton, Calif.). Reactions can also be analyzed by binding to Luminex biospheres (Luminex Corporation, Austin, Tex. Cai. H. (2000) Genomics 66(2):135-43). 
         [0116]    Other formats for nucleotide marker polymorphism detection include TaqMan™ (Applied Biosystems, Foster City, Calif.). Rolling circle (Hatch et al (1999) Genet. Anal. 15: 35-40, Qi et al (2001) Nucleic Acids Research Vol. 29 e116), fluorescence polarization (Chen, X., et al. (1999) Genome Research 9:492-498), SNaPShot (Applied Biosystems, Foster City, Calif.) (Makridakis, N. M. et al. (2001) Biotechniques 31:1374-80), oligo-ligation assay (Grossman, P. D., et al. (1994) Nucleic Acids Research 22:4527-4534), locked nucleic acids (LNATM, Link, Technologies LTD, Lanarkshire, Scotland, EP patent 1013661, U.S. Pat. No. 6,268,490), Invader Assay (Aclara Biosciences, Wilkinson, D. (1999) The Scientist 13:16), padlock probes (Nilsson et al.  Science  (1994), 265: 2085), Sequence-tagged molecular inversion probes (similar to padlock probes) from ParAllele Bioscience (South San Francisco, Calif.; Hardenbol, P. et al. (2003) Nature Biotechnology 21:673-678), Molecular Beacons (Marras, S. A. et al. (1999 Genet Anal. 14:151-156), the READIT™ SNP Genotyping System from Promega (Madison, Wis.) (Rhodes R. B. et al. (2001) Mol. Diagn. 6:55-61), Dynamic Allele-Specific Hybridization (DASH) (Prince, J. A. et al. (2001) Genome Research 11: 152-162), the Qbead™, system (quantum dot encoded microspheres conjugated to allele-specific oligonucleotides) (Xu H. et al. (2003) Nucleic Acids Research 31:e43), Scorpion primers (similar to molecular beacons except unimolecular) (Thelwell, N. et al. (2000) Nucleic Acids Research 28:3752-3761), and Magiprobe (a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator) (Yamane A. (2002) Nucleic Acids Research 30:e97). 
         [0117]    In addition, Rao, K. V. N. et al. ((2003) Nucleic Acids Research. 31:e66), recently reported a microsphere-based genotyping assay that detects SNPs directly from human genomic DNA. The assay involves a structure-specific cleavage reaction, which generates fluorescent signal on the surface of microspheres, followed by flow cytometry of the microspheres. With a slightly different twist on the Sequenom technology (MALDI), Sauer et al. ((2003) Nucleic Acids Research 31:e63) generate charge-tagged DNA (post PCR and primer extension), using a photocleavable linker. 
         [0118]    A method for identifying a nucleotide marker polymorphism also can be performed using a specific binding pair member. As used herein, the term “specific binding pair member” refers to a molecule that specifically binds or selectively hybridizes to another member of a specific binding pair. Specific binding pair members include, for example, probes, primers, polynucleotides, antibodies, etc. For example, a specific binding pair member includes a primer or a probe that selectively hybridizes to a target polynucleotide that includes a polymorphic site or that hybridizes to an amplification product generated using the target polynucleotide as a template. 
         [0119]    As used herein, the term “specific interaction,” or “specifically binds” or the like means that two molecules form a complex that is relatively stable under physiologic conditions. The term is used herein in reference to various interactions, including, for example, the interaction of an antibody that binds a polynucleotide that includes a polymorphic site or the interaction of an antibody that binds a polypeptide that includes an amino acid that is encoded by a codon that includes a polymorphic site. According to methods of the invention, an antibody can selectively bind to a polypeptide that includes a particular amino acid encoded by a codon that includes a polymorphic site. Alternatively, an antibody may preferentially bind a particular modified nucleotide that is incorporated into a polymorphic site for particular allelic differences at the polymorphic site, for example, using a primer extension assay. 
         [0120]    A specific interaction can be characterized by a dissociation constant of at least about 1×10-6 M, generally at least about 1×10-7 M, usually at least about 1×10-8 M, and particularly at least about 1×10-9 M or 1×10-10 M or less. A specific interaction generally is stable under physiological conditions, including, for example, conditions that occur in a living individual such as a human or other vertebrate or invertebrate, as well as conditions that occur in a cell culture such as used for maintaining mammalian cells or cells from another vertebrate organism or an invertebrate organism. Methods for determining whether two molecules interact specifically are well known and include, for example, equilibrium dialysis, surface plasmon resonance, and the like. 
         [0121]    The system can be a microfluidic device. Numerous microfluidic devices are known that include solid supports with microchannels (See e.g., U.S. Pat. Nos. 5,304,487, 5,110,745, 5,681,484, and 5,593,838). 
         [0122]    To facilitate detection, hybridization complexes can be modified to contain one or more labels. These labels can be incorporated by any of a number of means well known to those skilled in the art. Detectable labels suitable for use in the present invention include any composition detectable by spectroscopic, photochemical, biochemical, immunochemical, electrical, optical, or chemical means. Useful labels in the present invention include high affinity binding labels such as biotin for staining with labeled streptavidin or its conjugate, magnetic beads, fluorescent dyes (for example, fluorescein, Texas red, rhodamine, green fluorescent protein, and the like), radiolabels (for example 3H, 125I, 35S, 14C, or 32P), enzymes (for example horseradish peroxidase, alkaline phosphatase and others commonly used in an ELISA), epitope labels, and calorimetric labels such as colloidal gold, colored glass or plastic beads (for example polystyrene, polypropylene, latex, and the like). Means of detecting such labels are well known to those of skill in the art. Thus, for example, radiolabels can be detected using photographic film or scintillation counters, and fluorescent markers can be detected using a photodetector to detect emitted light. Enzymatic labels are typically detected by providing the enzyme with a substrate and detecting the reaction product produced by the action of the enzyme on the substrate, and calorimetric labels are detected by simply visualizing the colored label. One method uses colloidal gold as a label that can be detected by measuring light scattered from the gold. The label can be added to the amplification products prior to or after the hybridization. 
         [0123]    “Direct labels” are detectable labels that are directly attached to, or incorporated into, the nucleic acids prior to hybridization. In contrast, “indirect labels” are affixed to, or incorporated into the hybridization complex following hybridization. Often, the indirect label is attached to a binding moiety that has been attached to the amplified nucleic acid prior to hybridization. Thus, for example, the amplified nucleic acid can be biotinylated before hybridization. After hybridization, an avidin or streptavidin conjugated fluorophore will bind the biotin-bearing hybrid duplexes, providing a label that is easily detected. 
         [0124]    Means for detecting labeled nucleic acids hybridized to probes in an array are known to those skilled in the art. For example, when a colorimetric label is used, simple visualization of the label is sufficient. When radiolabeled probes are used, detection of the radiation (for example, with photographic film or a solid state detector) is sufficient. Detection of fluorescently labeled target nucleic acids can be accomplished by means of fluorescence microscopy. An array of hybridization complexes can be excited with a light source at the excitation wavelength of the particular fluorescent label of choice and the resulting fluorescence at the emission wavelength detected. The excitation light source can be, for example, a laser appropriate for the excitation of the fluorescent label. 
         [0125]    In a preferred embodiment, the hybridized nucleic acids are detected by detecting one or more labels attached to the sample nucleic acids. The labels may be incorporated by any of a number of means well known to those of skill in the art. However, in a preferred embodiment, the label is simultaneously incorporated during the amplification step in the preparation of the sample nucleic acids. Thus, for example, polymerase chain reaction (PCR) with labeled primers or labeled nucleotides will provide a labeled amplification product. In a preferred embodiment, transcription amplification, as described above, using a labeled nucleotide (e.g. fluorescein-labeled UTP and/or CTP) incorporates a label into the transcribed nucleic acids. 
         [0126]    Alternatively, a label may be added directly to the original nucleic acid sample (e.g., mRNA, polyA mRNA, cDNA, etc.) or to the amplification product after the amplification is completed. Means of attaching labels to nucleic acids are well known to those of skill in the art and include, for example nick translation or end-labeling (e.g. with a labeled RNA) by kinasing of the nucleic acid and subsequent attachment (ligation) of a nucleic acid linker joining the sample nucleic acid to a label (e.g., a fluorophore). 
         [0127]    Detectable labels suitable for use in the present invention include any composition detectable by spectroscopic, photochemical, biochemical, immunochemical, electrical, optical or chemical means. Useful labels in the present invention include biotin for staining with labeled streptavidin conjugate, magnetic beads (e.g., Dynabeads™), fluorescent dyes (e.g., fluorescein, texas red, rhodamine, green fluorescent protein, and the like), radiolabels (e.g., 3H, 125I, 35S, 14C, or 32P), enzymes (e.g., horse radish peroxidase, alkaline phosphatase and others commonly used in an ELISA), and coloimetric labels such as colloidal gold or colored glass or plastic (e.g., polystyrene, polypropylene, latex, etc.) beads. Patents teaching the use of such labels include U.S. Pat. Nos. 3,817,837; 3,850,752; 3,939,350; 3,996,345; 4,277,437; 4,275,149; and 4,366,241. 
         [0128]    An oligonucleotide probe array complementary to the reference sequence or subsequence thereof is immobilized on a solid support using one of the display strategies described below. For the purposes of clarity, much of the following description of the invention will use probe arrays where the reference sequence or subsequene thereof is selected from any one of the oligonucleotide marker sequences of Tables 2, 4 and/or 6 derived from horse or dog; however it should be recognized, as described previously, that probe arrays derived from other animal genomes may also be used, depending on the phenotypic trait being monitored, the availability of suitable primers and the like. 
         [0129]    The methods of this invention employ oligonucleotide arrays which comprise probes exhibiting complementarity to one or more selected reference sequences whose sequence is known. Typically, these arrays are immobilized in a high density array (“DNA on chip”) on a solid surface as described in U.S. Pat. No. 5,143,854 and PCT patent publication Nos. WO 90/15070, WO 92/10092 and WO 95/11995, each of which is incorporated herein by reference. 
         [0130]    In another embodiment, the present invention provides an isolated vector that includes a polynucleotide or oligonucleotide disclosed herein. The term “vector” refers to a plasmid, virus or other vehicle known in the art that has been manipulated by insertion or incorporation of a nucleic acid sequence. 
         [0131]    Methods that are well known in the art can be used to construct vectors, including in vitro recombinant DNA techniques, synthetic techniques, and in vivo recombination/genetic techniques (See, for example, the techniques described in Maniatis et al. 1989 Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, N.Y., incorporated herein in its entirety by reference). 
       Systems for Determining Multiple Characteristics in Animals Using the Simultaneous Identification of Polymorphisms in Biological Samples 
       [0132]    The present invention provides for systems to order and display the fluorescence and/or hybridization pattern, for example, of the assay plate utilized to detect a plurality of oligonucleotide marker polymorphisms. 
         [0133]      FIG. 1  is an exemplary reaction plate or panel  1000  upon which a plurality of samples or assays may be stored for processing in accordance with any of the techniques described above. In  FIG. 1 , panel  1000  includes an array of recesses  1002 , which may be implemented as wells or through-holes. A well is defined as a recess that extends partially through panel  1000 . For instance, a well does not form a hole through panel  1000 . A through-hole, on the other hand, is defined as a recess that extends entirely through panel  1000  from one opposing surface to another, thereby forming a hole through panel  1000 . 
         [0134]    In the embodiment of  FIG. 1 , recesses  1002  are grouped into a plurality of subarrays  1004 . Each subarray  1004  is shown to include a matrix of recesses  1002  having four rows and four columns for illustrative purposes. However, persons skilled in the art will recognize that subarrays  1004  can have any number of rows and columns or some other configuration. In fact, recesses  1002  need not be grouped into subarrays at all. 
         [0135]    Referring to  FIG. 1 , samples are placed in respective recesses  1002  of panel  1000 . Each sample may include a primer sequence pair, an oligonucleotide probe, a nucleic acid sample and/or a nucleotide marker sequence, to provide some examples. According to a first embodiment, each sample includes a respective primer sequence pair and a respective probe. Each of the primer sequences is capable of hybridizing to a sequence that is about 30 to 60 nucleotides upstream or downstream of a polymorphism present within a nucleotide marker sequence. In this embodiment, each of the primer sequence pairs flanks a polymorphism present within a nucleotide marker sequence. Moreover, each of the oligonucleotide probes is capable of hybridizing to a region that spans the polymorphism present within the nucleotide marker sequence. The plurality of primer sequence pairs and the plurality of probes is capable of detecting polymorphisms present within a plurality of nucleotide marker sequences. In this embodiment, the polymorphisms present within the plurality of nucleotide marker sequences correlate with at least two characteristics of an animal, such as parentage, identity, breed, sex, genotype and/or phenotype. 
         [0136]    According to a second embodiment, each sample includes a respective nucleotide marker sequence. Each of the nucleotide marker sequences includes a polymorphism and correlates with at least two characteristics, such as parentage, identity, breed, sex, genotype and/or phenotype. In this embodiment, each of the nucleotide marker sequences is complementary to a nucleotide sequence derived from one or more animals. 
         [0137]      FIG. 2  illustrates an exemplary processor-based system  1100 , which may be used to process samples according to an embodiment of the present invention. One or more aspects of the present invention may be implemented as programmable code. The programmable code may be provided in any of a variety of formats, including but not limited to C, C++, Java, and Visual Basic. Various embodiments of the invention are described in terms of exemplary processor-based system  1100 . After reading this description, it will become apparent to a person skilled in the art(s) how to implement the invention using other processor-based systems and/or computer architectures. 
         [0138]      FIG. 2  will be described with continued reference to reaction plate  1000  shown in  FIG. 1  for illustrative purposes. However, the scope of the present invention is not limited to the use of reaction plate  1000 . Any object capable of storing samples may be used in lieu of reaction plate  1000 . 
         [0139]    Referring now to  FIG. 2 , reaction plate  1000  is provided to plate receiving module  1116 , which secures reaction plate  1000  using a securing element. Samples may be provided to reaction plate  1000  before providing reaction plate  1000  to plate receiving module  1116 . Alternatively, plate receiving module  1116  may be used to manually or automatically provide the samples to reaction plate  1000 . 
         [0140]    Once the samples are loaded in plate receiving module, the samples may be processed in accordance with any of the techniques described above. For example, processor-based system  1100  may process the samples to identify characteristics, such as parentage, breed, identity, and/or phenotype, associated therewith. In another example, processor-based system  1100  may process the samples to identify SNPs therein. 
         [0141]    Processor-based system  1100  includes one or more processors, such as processor  1104 , to facilitate processing the samples. Processor  1104  may be any type of processor, including but not limited to a special purpose or a general purpose digital signal processor. Processor  1104  is connected to a communication infrastructure  1106  (for example, a bus or a network). 
         [0142]    Processor-based system  1100  also includes a main memory  1108 , preferably random access memory (RAM), and may also include a secondary memory  1110 . Secondary memory  1110  may include, for example, a hard disk drive  1112  and/or a removable storage drive  1114 , representing a floppy disk drive, a magnetic tape drive, an optical disk drive, etc. 
         [0143]    Removable storage drive  1114  reads from and/or writes to a removable storage unit  1118  in a well known manner. Removable storage unit  1118  represents a floppy disk, magnetic tape, optical disk, etc. As will be appreciated, removable storage unit  1118  includes a computer usable storage medium having stored therein computer software and/or data. 
         [0144]    In alternative implementations, secondary memory  1110  may include other similar means for allowing computer programs or other instructions to be loaded into processor-based system  1100 . Such means may include, for example, a removable storage unit  1122  and an interface  1120 . Examples of such means may include a program cartridge and cartridge interface (such as that found in video game devices), a removable memory chip (such as an EPROM or a PROM) and associated socket, and other removable storage units  1122  and interfaces  1120  which allow software and data to be transferred from removable storage unit  1122  to processor-based system  1100 . 
         [0145]    In  FIG. 2 , an optional communication interface  1124  allows software and data to be transferred between processor-based system  1100  and external devices. Examples of communication interface  1124  include but are not limited to a modem, a network interface (such as an Ethernet card), a communication port, a Personal Computer Memory Card International Association (PCMCIA) slot and card, etc. Software and data transferred via communication interface  1124  are in the form of signals  1128  which may be electronic, electromagnetic, optical, or other signals capable of being received by communication interface  1124 . These signals  1128  are provided to communication interface  1124  via a communication path  1126 . Communication path  1126  carries signals  1128  and may be implemented using wire or cable, fiber optics, a phone line, a cellular phone link, a radio frequency link, or any other suitable communication channel. For instance, communication path  1126  may be implemented using a combination of channels. 
         [0146]    In the embodiment of  FIG. 2 , processor-based system  1100  further includes a display interface  1102  that forwards graphics, text, and/or other information from communication infrastructure  1106  (or from a frame buffer not shown) for display on display unit  1130 . For instance, display unit  1130  may provide a graphical or textual representation of the results of processing the samples. Display unit may be a printer or a computer monitor, to provide some examples. 
         [0147]    In this document, the terms “computer program medium” and “computer usable medium” are used generally to refer to media such as removable storage unit  1118 , a hard disk installed in hard disk drive  1112 , and signals  1128 . These computer program products are means for providing software to processor-based system  1100 . 
         [0148]    Computer programs (also called computer control logic) are stored in main memory  1108  and/or secondary memory  1110 . Computer programs may also be received via communication interface  1124 . Such computer programs, when executed, enable processor-based system  1100  to implement the present invention as discussed herein. Accordingly, such computer programs represent controllers of processor-based system  1100 . Where the invention is implemented using software, the software may be stored in a computer program product and loaded into processor-based system  1100  using removable storage drive  1114 , hard disk drive  1112 , or communication interface  1124 , to provide some examples. 
         [0149]    In alternative embodiments, the invention can be implemented as control logic in hardware, firmware, or software or any combination thereof. 
         [0150]    The Examples provided herein illustrates the use of genotyping analysis to identify SNPs that can be used to determine parentage, identity, and/or phenotype of an animal (see Examples, infra). Information related to allele frequencies are utilized to correlate the presence of SNPS with a particular characteristic. The identification of particular SNPs in a target nucleic acid sequence. In some embodiments, forward oligonucleotide primers and reverse oligonucleotide primers were used to amplify specific target sequences prior to extension. 
         [0151]    The identification of a plurality of nucleotide marker polymorphisms, for example, can establish a “record” for individual animals, such that the unique set of nucleotide marker polymorphisms detected in an individual nucleic acid sample isolated from an animal can be used to link a genetic profile to that individual animal&#39;s identity. This information can be obtained by on-chip genetic testing and can be linked to a concurrently recorded biochemical ID marker which in turn can be cross-referenced with existing veterinary records to ensure authenticity. 
         [0152]    Many software programs for the analysis of nucleotide marker polymorphisms have been developed. Software programs to be used in the present invention include: The present disclosure incorporates the use of all of the software disclosed above used to classify animals into populations based on DNA polymorphisms as well as other software known in the art. 
         [0153]    The genetic profiling of animals plays an increasingly important role, not only in basic and applied clinical research, but also in the diagnosis of disease and in the assessment of predisposition to disease. A safe, reliable genetic testing protocol preferably will incorporate all relevant information relating to patient identification within individual tests. The present invention provides methods and compositions for linking the genetic profile obtained from the analysis of a patient&#39;s sample to a patient&#39;s identity. This correlation between a patient&#39;s genetic profile and identity is established concurrently with the genetic test or any diagnostic or prognostic test, on the basis of recording a genetic fingerprint or molecular identifier (ID). 
       Methods of Determining Diagnosis and Diseases 
       [0154]    The invention further provides a diagnostic method useful during diagnosis of a disease, e.g., which involves detecting the presence of a nucleotide marker polymorphisms in tissue or other cells or body fluid from an individual animal and comparing the measured presence with a standard nucleotide marker containing a polymorphism in normal tissue or body fluid, whereby the presence of a nucleotide containing a polymorphism compared to the standard is indicative of a disorder. 
         [0155]    By “assaying the presence of single nucleotide polymorphisms (SNPs) or polymorphism” is intended qualitatively or quantitatively measuring or estimating the present of SNPs, insertions, deletions, inversions and/or other mutations in a first biological sample either directly (e.g., by determining or estimating absolute presence of nucleotide containing a SNP) or relatively (e.g., by comparing to the disease associated with the presence of a nucleotide containing a SNP in a second biological sample). Preferably, the presence of a nucleotide containing a SNP in the first biological sample is measured or estimated and compared to a standard nucleotide marker containing a SNP, the standard being taken from a second biological sample obtained from an individual animal not having the disorder or being determined by averaging levels from a population of animals not having the disorder. As will be appreciated in the art, once the “standard” nucleotide marker containing a SNP is known, it can be used repeatedly as a standard for comparison. 
         [0156]    The method, compositions and systems according to the present invention provide for detection and diagnosis of diseases as further described below. 
         [0157]    Hyperkalemic periodic paralysis (HYPP) is an inherited disease of the muscle, which is caused by a genetic defect. In the muscle of affected horses, a point mutation exists in the sodium channel gene and is passed on to offspring. Sodium channels are “pores” in the muscle cell membrane which control contraction of the muscle fibers. When the defective sodium channel gene is present, the channel becomes “leaky” and makes the muscle overly excitable and contract involuntarily. The channel becomes “leaky” when potassium levels fluctuate in the blood. This may occur with fasting followed by consumption of a high potassium feed such as alfalfa. Hyperkalemia, which is an excessive amount of potassium in the blood, causes the muscles in the horse to contract more readily than normal. This makes the horse susceptible to sporadic episodes of muscle tremors or paralysis. 
         [0158]    This genetic defect has been identified in descendents of the American Quarter Horse sire, Impressive. The original genetic defect causing HYPP was a natural mutation that occurred as part of the evolutionary process. The majority of such mutations, which are constantly occurring, are not compatible with survival. However, the genetic mutation causing HYPP produced a functional, yet altered, sodium ion channel. This gene mutation is not a product of inbreeding. The gene mutation causing HYPP inadvertently became widespread when breeders sought to produce horses with heavy musculature. To date, confirmed cases of HYPP have been restricted to descendants of this horse. 
         [0159]    Severe Combined Immunodeficiency Disease (SCID) is an inherited disease specifically seen in pure and part-bred Arab horses. Foals afflicted with this condition have an enhanced susceptibility to infection and first show signs of disease at between two days and eight weeks of age. Clinical diagnosis of the disease is not straightforward as the symptoms, such as raised temperature, respiratory complications and diaharrea, are typical of new-born foals with a range of infections. SCID affected foals always die within the first six months of life, regardless of the level of veterinary care administered. SCID is therefore a distressing condition both for the animals involved and the owners and carers of the horses, and results in financial loss due to dead foals and veterinary expenses. 
         [0160]    Junctional epidermolysis bullosa (JEB) is an inherited disease that causes moderate to severe blistering of the skin and mouth epithelia, and sloughing of hooves in newborn foals. This condition is also known as red foot disease. Affected foals are typically born alive, but soon develop skin lesions at pressure points. The condition worsens with time and the foal eventually succumbs from severe infection or has to be euthanized. 
         [0161]    JEB in Belgian Draft horses has been shown to be the result of a specific mutation in a gene that affects the production of normal and healthy skin (F. Spirito et. al., J Invest Dermatol 119:684-691, 2002). To date, this mutation has been found only in Belgian Draft horses and derivatives of that breed. JEB is inherited as a recessive trait. Animals that carry two copies of the mutated gene (homozygous recessive) will develop the disease. Animals that carry one copy of the mutated gene and one copy of the normal gene (heterozygous) are carriers of JEB. Carriers do not develop the disease and have normal epithelium, but they have a 50% chance of passing on the mutation to their offspring. If N is used to represent the normal gene and J the mutated gene, an affected animal is designated J/J, a carrier animal is N/J and a normal animal is N/N. 
         [0162]    Comparative biochemical and histopathological evidence suggests that a deficiency in the glycogen branching enzyme, encoded by the GBE 1  gene, is responsible for a recently identified recessive fatal fetal and neonatal glycogen storage disease (GSD) in American Quarter Horses termed GSD IV. In the GBE1 cDNA sequences for control horses and affected foals, a C to A substitution at base 102 has been identified that results in a tyrosine (Y) to stop (X) mutation in codon 34 of exon 1. All 11 affected foals were homozygous for the X34 allele, their 11 available dams and sires were heterozygous, and all 16 control horses were homozygous for the Y34 allele. The previous findings of poorly branched glycogen, abnormal polysaccharide accumulation, lack of measurable GBE1 enzyme activity and immunodetectable GBE1 protein, coupled with the present observation of abundant GBE1 mRNA in affected foals, are all consistent with the nonsense mutation in the 699 amino acid GBE1 protein. The affected foal pedigrees have a common ancestor and contain prolific stallions that are likely carriers of the recessive X34 allele. Defining the molecular basis of equine GSD IV will allow for accurate DNA testing and the ability to prevent occurrence of this devastating disease affecting American Quarter Horses and related breeds. See e.g., Ward et al.,  Mammalian Genome  15(7): 570-577 (2004). 
         [0163]    Lethal White Overo (LWO) syndrome occurs when a horse is homozygous (OO) for the frame overo gene. This genetic disorder causes the intestinal system not to develop properly (involving aganglionosis of the bowel). The foal will die within the first 72 hours after birth when its first meals cannot be digested properly. The lethal white foal will be born almost pure white. This genetic abnormality is caused by a dinucleotide TC--&gt;AG mutation, which changes isoleucine to lysine of the EDNRB protein. 
         [0164]    Horses that do not have LWO syndrome can still be carriers of the LWO gene. When they are carriers of this gene, they are said to be heterozygous (nO) for the LWO gene and may pass it on to offspring. The heterozygous LWO gene in a horse occurs when the diploid (one copy from mother and one from father) of the LWO gene contains one frame overo copy and one non-frame overo copy and is often referred to as positive for frame overo. Since frame overo is a desirable quality and requires one frame overo copy, proper mating must be done to avoid possible loss due to lethal white overo while still achieving a high probability for the frame overo pattern. The way to avoid this problem is to avoid breeding frame overo to frame overo. 
         [0165]    In additional embodiments, the disease is selected from the group consisting of congenital myotonia, muscular dystrophy, globoid cell leucodystrophy, GM-gangliosidosis, Hemophilia B, hereditary cataracts, phosphofructokinase deficiency, thrombasthenic thrombopathia, retinal dystrophy, type-2 von Willerbrand&#39;s disease, and Type III von Willebrand. In certain other embodiments, the disease is selected from the group consisting of hypertrophic cardiomyopathy, polycystic kidney disease and mucopolysaccharidosis. 
         [0166]    Further information regarding disease may be identified by searching genetic databases or consulting periodicals or texts used in the vertinary industries and genetic testing industries. Thus, the diseases and sequences provided herein are intended to be nonlimiting with respect to scope. 
       Kits and Uses 
       [0167]    The invention also relates to kits, which can be used, for example, to perform a method of the invention. Thus, in one embodiment, the invention provides a kit for identifying a plurality of polymorphisms. Such a kit can contain, for example, an oligonucleotide probe(s), primer, or primer pair, or combinations thereof for identifying the nucleotide polymorphisms according to the present invention, following hybridization, primer extension, cleavage of the probe and fluorescence detection. Such oligonucleotides being useful, for example, to identify a polymorphism as disclosed herein; or can contain one or more nucleotide marker sequences corresponding to a characteristic selected from the group consisting of identity, parentage, breed, sex, genotype and phenotype. 
         [0168]    In addition, a kit of the invention can contain, for example, reagents for performing a method of the invention, including, for example, one or more detectable labels, which can be used to label a probe or primer or can be incorporated into a product generated using the probe or primer (e.g., an amplification product); one or more polymerases, which can be useful for a method that includes a primer extension or amplification procedure, or other enzyme or enzymes (e.g., a ligase or an endonuclease). The primers or probes can be included in a kit in a labeled form, for example with a label such as biotin or an antibody. In one embodiment, a kit of the invention provides a plurality of oligonucleotides of the invention, including one or more oligonucleotide probes or one or more primers, including forward and/or reverse primers, or a combination of such probes and primers or primer pairs. Such a kit also can contain probes and/or primers that conveniently allow a method of the invention to be performed using an assay plate or another substrate according to the invention. 
         [0169]    The kit can also include instructions for using the probes or primers to determine a plurality of nucleotide marker polymorphisms. 
         [0170]    The methods of the present invention are useful in the prevention of mishandling, mislabeling and switching of samples in the course of genetic testing. This invention prevents or corrects identification errors associated with mishandling, mislabeling and switching of samples by incorporating a genetic fingerprint or molecular identifier into the record of the genetic or other test, obtained, for example in the form of an image. In this way, an unambiguous link between that record and the animal&#39;s identity is established. The molecular identifier may serve to track and to confirm the identity of the sample, thereby providing a means for authentication. The methods of the present invention provide compositions and methods to create a genetic ID, also referred to herein as an ID, concurrently with the completion of a polymorphic genetic analysis. 
         [0171]    It will be understood by one of ordinary skill in the art that the compositions, methods and systems of the present invention can be utilized for cost-efficient and rapid analysis of a plurality of polymorphisms in other species of animals, including but not limited to humans, birds, reptiles, and amphibians. One of ordinary skill in the art can also utilize the present invention to detect other polymorphisms, such as SNPs, deletions, insertions and other mutations that are linked to diseases and/or phenotypes associated with the animals according to the invention. 
         [0172]    The practice of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, transgenic biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example. Molecular Cloning A Laboratory Manual, 2nd Ed., Sambrook et al., ed., Cold Spring Harbor Laboratory Press: (1989);  Molecular Cloning: A Laboratory Manual , Sambrook et al., ed., Cold Springs Harbor Laboratory, New York (1992),  DNA Cloning, D. N. Glover ed., Volumes I and II  (1985);  Oligonucleotide Synthesis , M. J. Gait ed. (1984); Mullis et al. U.S. Pat. No. 4,683,195 ; Nucleic Acid Hybridization , B. D. Hames &amp; S. J. Higgins eds. (1984);  Transcription And Translation , B. D. Hames &amp; S. J. Higgins eds. (1984);  Culture Of Animal Cells , R. I. Freshney, Alan R. Liss, Inc., (1987);  Immobilized Cells And Enzymes , IRL Press, (1986); B. Perbal,  A Practical Guide To Molecular Cloning  (1984); the treatise,  Methods In Enzymology , Academic Press, Inc. N.Y.;  Gene Transfer Vectors For Mammalian Cells , J. H. Miller and M. P. Calos eds., Cold Spring Harbor Laboratory (1987);  Methods In Enzymology , Vols. 154 and 155 (Wu et al. eds.);  Immunochemical Methods In Cell And Molecular Biology , Mayer and Walker, eds., Academic Press, London (1987);  Handbook Of Experimental Immunology , Volumes I-IV, D. M. Weir and C. C. Blackwell, eds., (1986);  Manipulating the Mouse Embryo , Cold Spring Harbor Laboratory Press, Cold Spring Harbor. N.Y., (1986); and in Ausubel et al.,  Current Protocols in Molecular Biology , John Wiley and Sons, Baltimore, Md. (1989). 
         [0173]    All of the references cited above, as well as all references cited herein, are incorporated herein by reference in their entireties. 
       EXAMPLES 
     Example 1 
     Simultaneous Identification of Multiple Characteristics Using 64 Horse Nucleotide Marker Sequences 
       [0174]    A nucleic acid sample isolated from an individual horse was analyzed to determine the presence of a plurality of nucleotide marker polymorphisms using an assay plate according to methods of the invention. On a single plate, 64 separate assays were simultaneously performed to determine the presence of a plurality of nucleotide marker polymorphisms, where the nucleotide marker polymorphisms comprise those as set forth in Table 2. 
         [0175]    In each assay, sequence-specific forward and reverse primers were hybridized to the nucleic sample according to the methods of the present invention. In addition, two modified oligonucleotide probes, a first oligonucleotide probe matching Allele 1 of the nucleotide marker sequence and a second oligonucleotide probe matching Allele 2 of the nucleotide marker sequence was combined with the nucleic acid sample. Each modified oligonucleotide probe contains a reporter dye at the 5′ end of the probe (e.g., a VIC® dye, or a FAM™ dye). A nonfluorescent quencher was attached at the 3′ end of the probe. Each of the first and second oligonucleotide probes were perfectly complementary to the invariant region of Allele 1 and Allele 2 of a nucleotide marker sequence according to Table 2. Finally, a DNA polymerase was added to the reaction in order that the oligonucleotide probe would be cleaved and its fluorescent reporter dye released upon matching with Allele 1 or Allele 2. The DNA polymerase contained within the assay mix can cleaved the oligonucleotide probe when it specifically hybridized to a PCR-amplified sequence present within the sample. 
         [0176]    The forward and reverse primers were hybridized to the nucleic acid sample. The nucleic acid sample was then amplified by PCR. Cleavage separates the reporter dye from the quencher dye, increasing fluorescence by the reporter. Thus, the fluorescence signal(s) generated by PCR amplification indicates the presence of a specific polymorphic allele within the nucleic acid sample. 
         [0177]    PCR reactions were performed using assay plates by thermal cycling using a commercial flat-block thermal cycler. Examples of the concentrations and amounts of reagents for the PCR reaction include but are not limited to those listed in Table 12. In this example, the concentration of DNA in the 5 μl sample was 30.3 ng/μl giving 1 ng of DNA in each well of the 64 well loading plate. The starting DNA stock solution can be modified based on the amount of DNA added to the sample. For example, if 1 μl of DNA is added to the sample, a 150 ng/μl stock solution would be required to obtain a final DNA concentration of 30 ng/μl. If 2 μl of DNA is added to the sample, a 75 ng/μl stock solution would be required to obtain a final DNA concentration of 30 ng/μl. Additional concentrations and amounts of reagents and DNA can be used in the methods of the present invention. 
         [0000]    
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
             
           
               
                 TABLE 12 
               
               
                   
               
               
                   
                 Stock 
                   
                 Final 
                 Volume 
                 Master 
               
               
                 Reagent 
                 conc. 
                 Units 
                 conc 
                 (ul) 
                 Mix Tube 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 ABI Taqman 
                 2 
                 x 
                 1 
                 2.5 
                 1584.00 
               
               
                 Master Mix 
               
               
                 BSA 
                 10 
                 mg/ml 
                 0.05 
                 0.025 
                 15.84 
               
               
                 Pluronic 
                 20 
                 % 
                 1 
                 0.25 
                 158.40 
               
               
                 F38 
               
               
                 Glycerol 
                 15 
                 % 
                 0.5 
                 0.16666667 
                 105.60 
               
               
                 H20 
                 — 
                 — 
                 — 
                 0.06 
                 36.96 
               
             
          
           
               
                 Master Mix total volume in each well of Black 
                 3 
                 1900.80 
               
               
                 MatriCal loading plate 
               
               
                 Add 2 uL DNA at 75 ng/uL 
                 2 
               
               
                 Total volume in Black MatriCal loading plate: 
                 5.00 
               
               
                   
               
             
          
         
       
     
         [0178]    The fluorescence output was subsequently read using a computer-based imaging system. The fluorescent output measurements were utilized to determine which particular alleles were present at the polymorphic position of each nucleotide marker sequence. Results of the assays listing the determination of both alleles for each nucleotide marker sequences are provided below in Table 13, where the assays were performed using individual samples isolated from 10 different animals. 
         [0000]    
       
         
               
               
               
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 13 
               
               
                   
               
               
                 Sample. 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 SampleID 
                 16317 
                 13306 
                 11986 
                 13218 
                 11987 
                 13219 
                 16317 
                 13306 
                 11986 
                 13218 
               
               
                   
               
             
             
               
                 ECA1_1- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS* 
                   
               
               
                 001.Genotype 
               
               
                   
               
               
                 ECA1_2- 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 DS 
                 A G 
                 A G 
                 DS 
               
               
                 002.Genotype 
               
               
                   
               
               
                 ECA1_3- 
                 A G 
                 A G 
                 A A 
                 A A 
                 A A 
                 A A 
                 A G 
                 A G 
                 A A 
                 DS 
               
               
                 003.Genotype 
               
               
                   
               
               
                 ECA2_1- 
                 DS 
                 DS 
                 DS 
                 DS 
                 C C 
                 C C 
                 DS 
                 C C 
                 DS 
                 DS 
               
               
                 004.Genotype 
               
               
                   
               
               
                 ECA2_2- 
                 G A 
                 G A 
                 A A 
                 G G 
                 G A 
                 G A 
                 G A 
                 G A 
                 A A 
                 DS 
               
               
                 005.Genotype 
               
               
                   
               
               
                 ECA2_3- 
                 T T 
                 T T 
                 T G 
                 T T 
                 T T 
                 T G 
                 T T 
                 T T 
                 T G 
                 DS 
               
               
                 006.Genotype 
               
               
                   
               
               
                 ECA3_1- 
                 C C 
                 T C 
                 T T 
                 T T 
                 T T 
                 T C 
                 C C 
                 T C 
                 T T 
                 DS 
               
               
                 007.Genotype 
               
               
                   
               
               
                 ECA3_2- 
                 A A 
                 DS 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 008.Genotype 
               
               
                   
               
               
                 ECA4_1- 
                 A G 
                 A A 
                 A A 
                 A G 
                 A A 
                 A G 
                 A G 
                 A A 
                 A A 
                 DS 
               
               
                 009.Genotype 
               
               
                   
               
               
                 ECA4_2- 
                 G G 
                 DS 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 DS 
               
               
                 010.Genotype 
               
               
                   
               
               
                 ECA5_1- 
                 A G 
                 A A 
                 A G 
                 A A 
                 A A 
                 A A 
                 A G 
                 A A 
                 A G 
                 DS 
               
               
                 011.Genotype 
               
               
                   
               
               
                 ECA5_2- 
                 A G 
                 DS 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
               
               
                 012.Genotype 
               
               
                   
               
               
                 ECA5_3- 
                 T C 
                 C C 
                 T C 
                 T C 
                 T C 
                 T C 
                 T C 
                 C C 
                 T C 
                 DS 
               
               
                 013.Genotype 
               
               
                   
               
               
                 ECA6_1- 
                 T G 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T G 
                 T T 
                 T T 
                 DS 
               
               
                 014.Genotype 
               
               
                   
               
               
                 ECA6_2- 
                 G G 
                 A A 
                 G A 
                 G G 
                 G G 
                 G A 
                 G G 
                 A A 
                 G A 
                 DS 
               
               
                 015.Genotype 
               
               
                   
               
               
                 ECA7_1- 
                 C C 
                 C T 
                 C T 
                 C C 
                 T T 
                 T T 
                 C C 
                 C T 
                 C T 
                 DS 
               
               
                 016.Genotype 
               
               
                   
               
               
                 ECA7_2- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 017.Genotype 
               
               
                   
               
               
                 ECA8_1- 
                 T T 
                 DS 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 C C 
                 T T 
                 DS 
               
               
                 018.Genotype 
               
               
                   
               
               
                 ECA8_2- 
                 C T 
                 C T 
                 C C 
                 C T 
                 C C 
                 C C 
                 C T 
                 C T 
                 C C 
                 DS 
               
               
                 019.Genotype 
               
               
                   
               
               
                 ECA9_1- 
                 T T 
                 C T 
                 C C 
                 C C 
                 C C 
                 C C 
                 T T 
                 C T 
                 C C 
                 DS 
               
               
                 020.Genotype 
               
               
                   
               
               
                 ECA9_2- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 021.Genotype 
               
               
                   
               
               
                 ECA10_1- 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A C 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 022.Genotype 
               
               
                   
               
               
                 ECA10_2- 
                 T T 
                 T T 
                 C C 
                 C T 
                 C C 
                 C C 
                 T T 
                 T T 
                 C C 
                 DS 
               
               
                 023.Genotype 
               
               
                   
               
               
                 ECA11_1- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
               
               
                 024.Genotype 
               
               
                   
               
               
                 ECA11_2- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 025.Genotype 
               
               
                   
               
               
                 ECA12_1- 
                 T C 
                 T T 
                 T C 
                 T C 
                 DS 
                 DS 
                 T C 
                 DS 
                 DS 
                 C C 
               
               
                 026.Genotype 
               
               
                   
               
               
                 ECA12_2- 
                 T T 
                 T T 
                 T C 
                 T C 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 T C 
               
               
                 027.Genotype 
               
               
                   
               
               
                 ECA13_1- 
                 A A 
                 DS 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A G 
                 A A 
                 DS 
               
               
                 028.Genotype 
               
               
                   
               
               
                 ECA13_2- 
                 G G 
                 DS 
                 A A 
                 A A 
                 G G 
                 A A 
                 G G 
                 A A 
                 A A 
                 G A 
               
               
                 029.Genotype 
               
               
                   
               
               
                 ECA14_1- 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 DS 
               
               
                 030.Genotype 
               
               
                   
               
               
                 ECA14_2- 
                 G G 
                 C C 
                 G G 
                 C G 
                 C G 
                 G G 
                 G G 
                 C C 
                 G G 
                 DS 
               
               
                 031.Genotype 
               
               
                   
               
               
                 ECA15_1- 
                 A G 
                 A A 
                 G G 
                 A A 
                 G G 
                 A A 
                 A G 
                 A A 
                 G G 
                 DS 
               
               
                 032.Genotype 
               
               
                   
               
               
                 ECA15_2- 
                 G G 
                 G G 
                 G G 
                 A G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 DS 
               
               
                 033.Genotype 
               
               
                   
               
               
                 ECA16_1- 
                 A A 
                 DS 
                 A G 
                 A A 
                 A G 
                 A G 
                 A A 
                 G G 
                 A G 
                 DS 
               
               
                 034.Genotype 
               
               
                   
               
               
                 ECA16_2- 
                 T T 
                 T T 
                 T C 
                 T C 
                 T C 
                 T C 
                 T T 
                 T T 
                 T C 
                 DS 
               
               
                 035.Genotype 
               
               
                   
               
               
                 ECA17_1- 
                 A A 
                 A A 
                 A A 
                 A A 
                 A G 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 036.Genotype 
               
               
                   
               
               
                 ECA17_2- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 037.Genotype 
               
               
                   
               
               
                 ECA18_1- 
                 A G 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A G 
                 A A 
                 A A 
                 DS 
               
               
                 038.Genotype 
               
               
                   
               
               
                 ECA19_1- 
                 T T 
                 T C 
                 C C 
                 C C 
                 C C 
                 C C 
                 T T 
                 T C 
                 C C 
                 DS 
               
               
                 039.Genotype 
               
               
                   
               
               
                 ECA20_1- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 040.Genotype 
               
               
                   
               
               
                 ECA21_1- 
                 DS 
                 A T 
                 A T 
                 A T 
                 A T 
                 A T 
                 A T 
                 A T 
                 A T 
                 DS 
               
               
                 041.Genotype 
               
               
                   
               
               
                 ECA22_1- 
                 T T 
                 DS 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 T T 
                 DS 
               
               
                 042.Genotype 
               
               
                   
               
               
                 ECA23_1- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 043.Genotype 
               
               
                   
               
               
                 ECA24_1- 
                 T C 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 T T 
                 T T 
                 DS 
               
               
                 044.Genotype 
               
               
                   
               
               
                 ECA25_1- 
                 T T 
                 T T 
                 T C 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 DS 
               
               
                 045.Genotype 
               
               
                   
               
               
                 ECA26_1- 
                 C C 
                 C C 
                 C T 
                 C T 
                 C T 
                 C T 
                 C C 
                 C C 
                 C T 
                 DS 
               
               
                 046.Genotype 
               
               
                   
               
               
                 ECA27_1- 
                 C T 
                 T T 
                 T T 
                 C T 
                 C T 
                 T T 
                 C T 
                 T T 
                 T T 
                 DS 
               
               
                 047.Genotype 
               
               
                   
               
               
                 ECA28_1- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 048.Genotype 
               
               
                   
               
               
                 ECA29_REPL- 
                 C C 
                 C T 
                 T T 
                 C T 
                 T T 
                 T T 
                 C C 
                 C T 
                 T T 
                 DS 
               
               
                 049.Genotype 
               
               
                   
               
               
                 ECA30_1- 
                 A G 
                 DS 
                 A A 
                 A A 
                 A A 
                 A A 
                 A G 
                 A A 
                 A A 
                 DS 
               
               
                 050.Genotype 
               
               
                   
               
               
                 ECA31_1- 
                 C T 
                 T T 
                 C T 
                 T T 
                 C T 
                 T T 
                 C T 
                 T T 
                 C T 
                 C C 
               
               
                 051.Genotype 
               
               
                   
               
               
                 ECA31_2- 
                 A A 
                 G G 
                 G A 
                 A A 
                 G A 
                 G G 
                 A A 
                 G G 
                 G A 
                 DS 
               
               
                 052.Genotype 
               
               
                   
               
               
                 ECAX_1- 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 053.Genotype 
               
               
                   
               
               
                 ECA1_4- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 065.Genotype 
               
               
                   
               
               
                 E_AGOUTI_10. 
                 GAAA 
                 * * 
                 GAAA 
                 GAAA 
                 GAAA 
                 * * 
                 GAAA 
                 * * 
                 GAAA 
                 DS 
               
               
                 Genotype 
                 AGAA 
                   
                 AGAA 
                 AGAA 
                 AGAA 
                   
                 AGAA 
                   
                 AGAA 
               
               
                   
                 GCA* 
                   
                 GCA* 
                 GCA* 
                 GCA* 
                   
                 GCA* 
                   
                 GCA* 
               
               
                   
               
               
                 CREAM- 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
               
               
                 CRE.Genotype 
               
               
                   
               
               
                 HORSE_RED- 
                 C C 
                 T T 
                 C C 
                 C C 
                 C C 
                 C T 
                 C C 
                 T T 
                 C C 
                 C C 
               
               
                 MC1R.Genotype 
               
               
                   
               
               
                 SABINO- 
                 DS 
                 DS 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T A 
               
               
                 SABI.Genotype 
               
               
                   
               
               
                 SILVERH- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 SILH.Genotype 
               
               
                   
               
               
                 TOBIANO- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 TOB.Genotype 
               
               
                   
               
               
                 HYPP_NEW- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 HYP.Genotype 
               
               
                   
               
               
                 HORSE_LWO- 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 DS 
               
               
                 LWO.Genotype 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
                 TC 
               
               
                   
               
               
                 HORSE_JEB- 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 DS 
               
               
                 JEB.Genotype 
               
               
                   
               
               
                 HORSE_GBE1- 
                 C C 
                 C C 
                 DS 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C A 
               
               
                 GBE1.Genotype 
               
               
                   
               
               
                 *DS: Polymorphic alleles were read under different stringency conditions with reliable results. 
               
             
          
         
       
     
         [0179]    Each sample was tested against the 64 markers listed in Table 2. The two oligonucleotide probe contained VIC® and FAM™, respectively, at the 5′ end of the probes. The control was no template. 
         [0180]    Assays as described above were performed using additional samples isolated from 10 other animals. Results of the additional assays listing the determination of both alleles for each nucleotide marker sequences are provided below in Table 14: 
         [0000]    
       
         
               
               
               
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 14 
               
               
                   
               
               
                 Sample. 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 SampleID 
                 11987 
                 13219 
                 10740 
                 15849 
                 15051 
                 15850 
                 16297 
                 16298 
                 10740 
                 NTC 
               
               
                   
               
             
             
               
                 ECA1_1- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C T 
                 C C 
                 DS 
                   
               
               
                 001.Genotype 
               
               
                   
               
               
                 ECA1_2- 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
                 DS 
               
               
                 002.Genotype 
               
               
                   
               
               
                 ECA1_3- 
                 A A 
                 A A 
                 A A 
                 A G 
                 A G 
                 A G 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 003.Genotype 
               
               
                   
               
               
                 ECA2_1- 
                 DS 
                 C C 
                 C C 
                 G C 
                 DS 
                 DS 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 004.Genotype 
               
               
                   
               
               
                 ECA2_2- 
                 G A 
                 G A 
                 A A 
                 G G 
                 G A 
                 G G 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 005.Genotype 
               
               
                   
               
               
                 ECA2_3- 
                 T T 
                 T G 
                 T T 
                 T T 
                 T T 
                 T T 
                 T G 
                 T T 
                 T T 
                 DS 
               
               
                 006.Genotype 
               
               
                   
               
               
                 ECA3_1- 
                 T T 
                 T C 
                 T C 
                 T T 
                 T C 
                 T T 
                 T T 
                 T C 
                 T C 
                 DS 
               
               
                 007.Genotype 
               
               
                   
               
               
                 ECA3_2- 
                 A A 
                 A A 
                 A A 
                 A G 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 008.Genotype 
               
               
                   
               
               
                 ECA4_1- 
                 A A 
                 A G 
                 G G 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 G G 
                 DS 
               
               
                 009.Genotype 
               
               
                   
               
               
                 ECA4_2- 
                 G G 
                 G G 
                 G G 
                 G G 
                 G A 
                 DS 
                 G G 
                 G A 
                 G G 
                 DS 
               
               
                 010.Genotype 
               
               
                   
               
               
                 ECA5_1- 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 011.Genotype 
               
               
                   
               
               
                 ECA5_2- 
                 A G 
                 A G 
                 A G 
                 A G 
                 DS 
                 A G 
                 A G 
                 A G 
                 A G 
                 A G 
               
               
                 012.Genotype 
               
               
                   
               
               
                 ECA5_3- 
                 T C 
                 T C 
                 T C 
                 C C 
                 T T 
                 T C 
                 T C 
                 T C 
                 T C 
                 DS 
               
               
                 013.Genotype 
               
               
                   
               
               
                 ECA6_1- 
                 T T 
                 T T 
                 G G 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 G G 
                 DS 
               
               
                 014.Genotype 
               
               
                   
               
               
                 ECA6_2- 
                 G G 
                 G A 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 DS 
               
               
                 015.Genotype 
               
               
                   
               
               
                 ECA7_1- 
                 T T 
                 T T 
                 C C 
                 T T 
                 C T 
                 C T 
                 C T 
                 T T 
                 C C 
                 DS 
               
               
                 016.Genotype 
               
               
                   
               
               
                 ECA7_2- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 017.Genotype 
               
               
                   
               
               
                 ECA8_1- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 018.Genotype 
               
               
                   
               
               
                 ECA8_2- 
                 C C 
                 C C 
                 C C 
                 C T 
                 C C 
                 C T 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 019.Genotype 
               
               
                   
               
               
                 ECA9_1- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C T 
                 C T 
                 C C 
                 DS 
               
               
                 020.Genotype 
               
               
                   
               
               
                 ECA9_2- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 021.Genotype 
               
               
                   
               
               
                 ECA10_1- 
                 A A 
                 A C 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 022.Genotype 
               
               
                   
               
               
                 ECA10_2- 
                 C C 
                 C C 
                 T T 
                 C T 
                 C C 
                 C C 
                 C T 
                 C T 
                 T T 
                 DS 
               
               
                 023.Genotype 
               
               
                   
               
               
                 ECA11_1- 
                 T T 
                 T T 
                 T C 
                 T C 
                 T C 
                 T C 
                 T C 
                 T C 
                 T C 
                 DS 
               
               
                 024.Genotype 
               
               
                   
               
               
                 ECA11-2- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 025.Genotype 
               
               
                   
               
               
                 ECA12_1- 
                 T C 
                 DS 
                 DS 
                 T C 
                 T C 
                 T C 
                 T C 
                 DS 
                 T C 
                 C C 
               
               
                 026.Genotype 
               
               
                   
               
               
                 ECA12_2- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 T T 
                 T C 
                 T T 
                 T T 
                 DS 
               
               
                 027.Genotype 
               
               
                   
               
               
                 ECA13_1- 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 028.Genotype 
               
               
                   
               
               
                 ECA13_2- 
                 G G 
                 G A 
                 A A 
                 G A 
                 G G 
                 A A 
                 A A 
                 A A 
                 A A 
                 G A 
               
               
                 029.Genotype 
               
               
                   
               
               
                 ECA14_1- 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 DS 
               
               
                 030.Genotype 
               
               
                   
               
               
                 ECA14_2- 
                 C G 
                 G G 
                 G G 
                 G G 
                 C G 
                 G G 
                 G G 
                 C C 
                 G G 
                 DS 
               
               
                 031.Genotype 
               
               
                   
               
               
                 ECA15_1- 
                 G G 
                 A A 
                 A G 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A G 
                 DS 
               
               
                 032.Genotype 
               
               
                   
               
               
                 ECA15_2- 
                 G G 
                 G G 
                 G G 
                 G G 
                 A G 
                 G G 
                 G G 
                 G G 
                 G G 
                 DS 
               
               
                 033.Genotype 
               
               
                   
               
               
                 ECA16_1- 
                 A G 
                 A G 
                 G G 
                 A A 
                 G G 
                 G G 
                 A G 
                 A G 
                 G G 
                 DS 
               
               
                 034.Genotype 
               
               
                   
               
               
                 ECA16_2- 
                 T C 
                 T C 
                 T T 
                 T C 
                 T T 
                 T T 
                 T C 
                 T C 
                 T T 
                 DS 
               
               
                 035.Genotype 
               
               
                   
               
               
                 ECA17_1- 
                 A G 
                 A A 
                 G G 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 G G 
                 DS 
               
               
                 036.Genotype 
               
               
                   
               
               
                 ECA17_2- 
                 T T 
                 T T 
                 T C 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 DS 
               
               
                 037.Genotype 
               
               
                   
               
               
                 ECA18_1- 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 038.Genotype 
               
               
                   
               
               
                 ECA19_1- 
                 C C 
                 C C 
                 T T 
                 C C 
                 C C 
                 T C 
                 C C 
                 C C 
                 T T 
                 DS 
               
               
                 039.Genotype 
               
               
                   
               
               
                 ECA20_1- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 040.Genotype 
               
               
                   
               
               
                 ECA21_1- 
                 A T 
                 A T 
                 A A 
                 A T 
                 A T 
                 A T 
                 A T 
                 A T 
                 A T 
                 DS 
               
               
                 041.Genotype 
               
               
                   
               
               
                 ECA22_1- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 042.Genotype 
               
               
                   
               
               
                 ECA23_1- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 043.Genotype 
               
               
                   
               
               
                 ECA24_1- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 044.Genotype 
               
               
                   
               
               
                 ECA25_1- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T C 
                 T C 
                 C C 
                 T C 
                 T T 
                 DS 
               
               
                 045.Genotype 
               
               
                   
               
               
                 ECA26_1- 
                 C T 
                 C T 
                 C C 
                 C T 
                 DS 
                 C T 
                 C T 
                 C T 
                 C C 
                 DS 
               
               
                 046.Genotype 
               
               
                   
               
               
                 ECA27_1- 
                 C T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
               
               
                 047.Genotype 
               
               
                   
               
               
                 ECA28_1- 
                 C C 
                 C C 
                 C C 
                 C T 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 048.Genotype 
               
               
                   
               
               
                 ECA29_REPL- 
                 T T 
                 T T 
                 C T 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 C T 
                 DS 
               
               
                 049.Genotype 
               
               
                   
               
               
                 ECA30_1- 
                 A A 
                 A A 
                 A G 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A G 
                 DS 
               
               
                 050.Genotype 
               
               
                   
               
               
                 ECA31_1- 
                 C T 
                 T T 
                 C T 
                 C T 
                 C T 
                 C T 
                 T T 
                 C T 
                 C T 
                 DS 
               
               
                 051.Genotype 
               
               
                   
               
               
                 ECA31_2- 
                 G A 
                 G G 
                 G A 
                 G G 
                 G A 
                 G A 
                 G G 
                 G G 
                 G A 
                 DS 
               
               
                 052.Genotype 
               
               
                   
               
               
                 ECAX_1- 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 A A 
                 DS 
               
               
                 053.Genotype 
               
               
                   
               
               
                 ECA1_4- 
                 T C 
                 T T 
                 T T 
                 T T 
                 DS 
                 T T 
                 T T 
                 T C 
                 T T 
                 DS 
               
               
                 065.Genotype 
               
               
                   
               
               
                 E_AGOUTI_10. 
                 GAAA 
                 * * 
                 GAAA 
                 GAAA 
                 GAAA 
                 * * 
                 GAAA 
                 * * 
                 GAAA 
                 DS 
               
               
                 Genotype 
                 AGAA 
                   
                 AGAA 
                 AGAA 
                 AGAA 
                   
                 AGAA 
                   
                 AGAA 
               
               
                   
                 GCA* 
                   
                 GCA* 
                 GCA* 
                 GCA* 
                   
                 GCA* 
                   
                 GCA* 
               
               
                   
               
               
                 CREAM- 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 G G 
                 DS 
               
               
                 CRE.Genotype 
               
               
                   
               
               
                 HORSE_RED- 
                 C C 
                 C T 
                 C C 
                 C T 
                 C C 
                 T T 
                 C T 
                 T T 
                 C C 
                 DS 
               
               
                 MC1R.Genotype 
               
               
                   
               
               
                 SABINO- 
                 T T 
                 T T 
                 T T 
                 T T 
                 T T 
                 DS 
                 T T 
                 T T 
                 T T 
                 T A 
               
               
                 SABI.Genotype 
               
               
                   
               
               
                 SILVERH- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C T 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 SILH.Genotype 
               
               
                   
               
               
                 TOBIANO- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 TOB.Genotype 
               
               
                   
               
               
                 HYPP_NEW- 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 HYP.Genotype 
               
               
                   
               
               
                 HORSE_LWO- 
                 TC 
                 TC 
                 TC 
                 TC 
                 DS 
                 TC 
                 TC 
                 TC 
                 TC 
                 DS 
               
               
                 LWO.Genotype 
                 TC 
                 TC 
                 TC 
                 TC 
                   
                 TC 
                 TC 
                 TC 
                 TC 
               
               
                   
               
               
                 HORSE_JEB- 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 * C 
                 DS 
               
               
                 JEB.Genotype 
               
               
                   
               
               
                 HORSE_GBE1- 
                 DS 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 C C 
                 DS 
               
               
                 GBE1.Genotype 
               
               
                   
               
               
                 *** 
               
             
          
         
       
     
         [0181]    Again, each sample was tested against the 64 markers listed in Table 2. The two oligonucleotide probe contained VIC® and FAM™, respectively, at the 5′ end of the probes. The control was no template. 
         [0182]    The presence of particular alleles as disclosed in Tables 8 and 9 were utilized to determine the presence of at least two characteristics selected from the group consisting of parentage, identity and/or phenotype using information available to one of ordinary skill in the art. 
       Example 2 
     Simultaneous Identification of Multiple Characteristics Using 128 Horse Nucleotide Marker Sequences 
       [0183]    A nucleic acid sample isolated from an individual horse is analyzed to determine the presence of a plurality of nucleotide marker polymorphisms using an assay plate according to methods of the invention. On a single plate, 128 separate assays are simultaneously performed to determine the presence of a plurality of nucleotide marker polymorphisms, where the nucleotide marker polymorphisms comprise those as set forth in Tables 2 and 4. 
         [0184]    The assay is performed according to the methods described in Example 1 above. Results of the assays as measured by fluorescent output are tabulated. 
       Example 3 
     Simultaneous Identification of Multiple Characteristics Using Horse and Dog Nucleotide Marker Sequences 
       [0185]    A nucleic acid sample isolated from individual horses, cattle, cats and dogs are analyzed to determine the presence of a plurality of nucleotide marker polymorphisms using an assay plate according to methods of the invention for each individual animal. On a single plate, up to 3000 separate assays are simultaneously performed to determine the presence of a plurality of nucleotide marker polymorphisms, where the nucleotide marker polymorphisms comprise those as set forth in Tables 2, 4, 6 and 8. 
         [0186]    The assay is performed according to the methods described in Example 1 above. Results of the assays as measured by fluorescent output are tabulated. 
       Example 4 
     Raw Data Plots Showing Examples of Markers for Parentage, Identity, Sex, Phenotype and/or Genotype and Breed Determination 
       [0187]      FIGS. 3A-6C  provide examples of raw data plots generated by a processor based system from individual markers depicting the presence of nucleotide marker polymorphism using an assay plate according to methods of the invention for groups of 47 and 23 animals respectively comprising cat, dog, horse, and cattle species. The plots give examples of identity and parentage, genotype and/or phenotype including disease diagnostics and traits like color, sex determination where females are homozygous and males are heterozygous, and breed determination. Each individual marker was simultaneously analyzed along with 63 or 127 other markers comprising all 5 of the (i) parentage; (ii) identity; (iii) sex, (iv) genotype and (v) phenotype