Abstract:
‘DaisySL’ is a mid-season maturing diploid mandarin that combines medium-large sized fruit of excellent quality and production with very low seed content even in mixed plantings. It would likely be successful in the mid-season marketing window that currently has very few low-seeded, high quality cultivars.

Description:
LATIN NAME OF THE GENUS AND SPECIES 
       [0001]    The mandarin cultivar of this invention is botanically identified as  Citrus reticulata.    
       VARIETY DENOMINATION 
       [0002]    The variety denomination is ‘DaisySL’. 
       BACKGROUND OF THE INVENTION 
       [0003]    This invention relates to a new and distinctive mandarin cultivar designated ‘DaisySL’, which was developed at Riverside, Calif., and derived from an irradiated bud of ‘Daisy’ mandarin. ‘Daisy’ mandarin was produced in Indio, Calif. in 1963 by J. R. Furr from a conventional hybridization of 2n ‘Fortune’×2n ‘Fremont’×mandarin. ‘Fortune’ mandarin was itself produced from a conventional hybridization of 2n ‘Clementine’×2n ‘Dancy’ mandarin made by J. R. Furr in Indio, Calif. in 1954 and released in 1964. ‘Fremont’ mandarin was obtained from a conventional hybridization of 2n ‘Clementine’ mandarin×2n ‘Ponkan’ mandarin made by P. C. Reece in Orlando, Fla. in 1948 and later fruited, selected and released by J. R. Furr in Indio, Calif. in 1964. 
         [0004]    Irradiation of budwood from registered ‘Daisy’ trees in Lindcove, Calif., was accomplished in June, 1997 at Riverside using 50 Gray units of gamma irradiation from a Cobalt-60 irradiation source. Buds from this irradiation were propagated onto various rootstocks in the greenhouse at Riverside where they were grown to field-plantable-sized trees. These trees were planted in June 1998 at Riverside. Fruit production and evaluation began in 2001. One selection from this irradiated population (propagated on Carrizo citrange rootstock) distinguished itself from the others in having very low seed counts in comparison to the original ‘Daisy’ cultivar, and with the excellent fruit quality and normal fruit production characteristics of the ‘Daisy’ parent. After two seasons of fruiting this selection, designated as ‘Daisy IR 1’, was selected for further trials and in January 2003 buds were taken and propagated onto Carrizo and C35 citrange rootstock. Budwood was also sent in April 2003 for evaluation of disease status and elimination of viruses and other pathogens as needed to establish trial plantings. ‘DaisySL’ was known throughout experimental evaluation as Daisy IR1-(for DAISY IRradiated selection #1). Twenty trees were planted at Riverside in June 2003. Fruit production on these 20 trees commenced in 2006. In June 2004 two trees of ‘DaisySL’, which had been produced from budwood that had tested and certified as tristeza-free, were sent Lindcove, Calif. where they were planted in the citrus breeding block. In June 2004 seventy-two trees, produced in Lindcove, Calif. were planted (twelve trees each) at six sites, Arvin, Irvine, Lindcove, Oasis, Santa Paula and Woodlake, Calif. All trials were propagated equally on Carrizo and C35 citrange rootstocks. Fruit production of these propagated trees commenced in 2006 (a few trees at each site) and 2007 (all trees at all sites). The properties of ‘DaisySL’ were found to be true to type and transmissible by asexual reproduction in comparing these plantings with the original ‘DaisySL’ selection. 
       BRIEF SUMMARY OF THE INVENTION 
       [0005]    ‘DaisySL’ is a mid-season maturing diploid mandarin that combines medium-large sized fruit of excellent quality and production with very low seed content even in mixed plantings. It would likely be successful in the mid-season marketing window that currently has very few low-seeded, high quality cultivars. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0006]    The Figures depict various characteristics of ‘DaisySL’. 
           [0007]      FIG. 1  shows an eight-year-old ‘mother’ tree of ‘DaisySL’ on Carrizo  citrange  rootstock. 
           [0008]      FIG. 2  shows a three-year-old ‘DaisySL’ tree on Carrizo citrange rootstock at Santa Paula, Calif. (first crop). 
           [0009]      FIG. 3  shows a bud-union of nine-year-old ‘DaisySL’ on Carrizo citrange rootstock. 
           [0010]      FIG. 4  shows the fruit of ‘DaisySL’ taken at Riverside in February. 
           [0011]      FIG. 5  shows fruit clusters of ‘DaisySL’ taken at Santa Paula in February. 
           [0012]      FIG. 6  shows leaves of ‘DaisySL’ 
           [0013]      FIG. 7  shows open and closed flowers of ‘DaisySL’ mandarin 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0014]    ‘DaisySL’ is a mandarin selection developed at the University of California Riverside from an irradiated bud of the diploid mandarin cultivar ‘Daisy’, a mid-season maturing variety. ‘DaisySL’ distinguishes itself by being very low seeded (2.2 seeds/fruit) in all situations of cross-pollination, differing from ‘Daisy’ which will set from 16-25 seeds/fruit in cross-pollinated situations. Evaluation of ‘DaisySL’ mandarin began on the original tree at Riverside in 2001 and has continued annually until the present. In Riverside, Calif. ‘DaisySL’ fruit matures in winter (early-December). ‘DaisySL’ holds its fruit quality characteristics through February. Fruit size is moderately large (68 mm) averaging 135 grams per fruit. Fruit are slightly obconate in shape with a very deep orange rind color and an extremely smooth rind texture. Flesh color is very deep orange and finely-textured, fruit are juicy, with a rich, sweet and very distinctive flavor when mature. Fruit are only moderately easy to peel. Tree growth habit is spreading with excellent production commencing in the third year after planting. Alternate bearing can be a problem in trees that are not culturally managed to reduce this tendency. 
         [0015]    Cultural practices ‘DaisySL’ mandarin can be grown according to accepted cultural practices for most mandarin varieties including planting densities of 150-250 trees per acre (375-625 trees/ha), normal fertilization and pest control practices, and the use of standard rootstocks for mandarins. Pruning may enhance production and health of the tree if applied after the second year of full fruit production. Other rootstocks adapted to more marginal growing conditions of salinity, high pH or very heavy soils may be useful in those conditions.
   Comparison with existing mandarins:   
 
         [0017]    Mid to late season maturing mandarin cultivars in production include ‘Daisy’ mandarin (the original cultivar from which ‘DaisySL’ was derived), ‘W. Murcott’ (Afourer), ‘Fortune’ mandarin, Ortanique mandarin, ‘Temple’ tangor, ‘Dancy’ mandarin ‘Minneola’ tangelo, and the mid-season Clementina selections ‘Hernandina’ and ‘Nour’. All of these cultivars will be seedy if grown in the presence of a pollenizer. Some, including the Clementina selections, ‘Fortune’, ‘Ortanique’ and ‘Page’ mandarins will have few seeds if no pollenizer is present. Recently released mid to late season cultivars that are very low-seeded include ‘Tango’ mandarin, ‘Gold Nugget’ mandarin, ‘TDE2’ mandarin hybrid (Shasta Gold®) ‘TDE3’ mandarin hybrid (Tahoe Gold®), and ‘TDE4’ mandarin hybrid (Yosemite Gold®). ‘DaisySL’ differs from these cultivars in being earlier maturing, having fruit with a smoother rind texture, and a lesser ability to maintain fruit on the tree or in storage for an extended period. Trees of ‘DaisySL’ show similar alternate bearing characteristics to these cultivars. Additional differences (summarized in Table 6) distinguish it from each of these cultivars. 
         [0018]    ‘DaisySL’ mandarin exhibits low seed numbers (&lt;2.5 seeds per fruit) under all conditions of cross-pollination. Additionally, preliminary evaluations indicate that pollen from ‘DaisySL’ has low germination rates in culture (˜10-20%) and appears not likely to cause high seed numbers in other mandarins, specifically ‘Tango’, ‘W. Murcott’ and Clementines. A comparison of ‘DaisySL’ with other low-seeded mid and late-season mandarins is provided in Table 6 below. ‘DaisySL’ is distinctive and superior in having outstanding flavor, an exceptionally smooth rind, reduced alternate bearing, and larger fruit size preferred in some markets, although it retains the tendency of its parent ‘Daisy’ for fruit to split at levels approaching 20% in bad years.
   Trees, foliage, and flowers:   
 
         [0020]    Tree size, growth and fruit production characteristics and fruit quality characteristics have been compared in these evaluations to ‘Daisy’ mandarin from the same field block. Six-year-old ‘DaisySL’ trees in trials at Riverside, and four-year-old trees at the other six sites have been evaluated for from two to four years of fruiting (see Tables 1 and 2). Tree size and growth characteristics of ‘DaisySL’ have been consistent with ‘Daisy’ throughout the evaluations. Growth of both the ‘Daisy’ and the ‘DaisySL’ selection has been quite spreading (characterized as ‘leggy’) in the first several years of growth followed by a tendency to grow into a more spherical, slightly drooping shape in ensuing years. The nine-year-old ‘DaisySL’ tree at Riverside on Carrizo citrange rootstock is 3.3 m high and 3.7 m wide with a normal upright growth habit yielding a canopy volume of 23.65 m 3 . In comparison nine-year-old ‘Daisy’ control trees have averaged 3.2 m tall and 3.6 m wide yielding a canopy volume of 21.7 m 3  on Carrizo citrange rootstock. Scion circumference for ‘DaisySL’ on Carrizo rootstock was 38.5 cm with the rootstock circumference 54.7 cm. Scion circumference for the nine-year old ‘Daisy’ trees averaged 36.9 cm on Carrizo rootstock and 38.2 cm on C35 rootstock. In the younger multi-location trials five-year-old ‘DaisySL’ trees on Carrizo rootstock have averaged 2.8 m in height and 3.0 m in diameter with canopy volumes of 8.48 m 3  and trees on C35 rootstock averaged 2.9 m in height and 2.7 m in diameter with canopy volumes of 7.48 m 3 . Leaves of ‘DaisySL’ are moderately large for a mandarin (86.1 mm in length×47.9 mm in width), ovate in shape and concave in cross-section, with an acute apex with weak emargination and a convex base and are dark-green in color (adaxial—RHS Green 139A, abaxial—RHS Green 137C). Petioles are medium in length (9.5 mm) and normally lack wings. The selection lacks thorns. Flowers of ‘DaisySL’ are hermaphroditic with greenish-white petals and yellowish anthers and are borne in clusters. Pollen viability for ‘DaisySL’ is moderately low (10-20% germination vs. ˜75% germination for ‘Daisy’), and pollen production is reduced (30-40% that of ‘Daisy’) in comparison to ‘Daisy’. 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 Tree, leaf, flower and seed characteristics (for nine-year-old tree) of 
               
               
                 ‘DaisySL’ mandarin 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 1. 
                 Tree height 
                 3.7 m 
               
               
                 2. 
                 Crown diameter 
                 3.8 m 
               
               
                 3. 
                 Crown shape 
                 Spreading, open 
               
               
                 4. 
                 Trunk circumference 
                 38.5 cm 
               
               
                   
                 (on Carrizo rootstock) 
                   
               
               
                 5. 
                 Bud-union characteristics 
                 Slightly benched 
               
               
                   
                 (citranges) 
                   
               
               
                 6. 
                 Rootstock-scion compatibility 
                 Excellent (with citranges) 
               
               
                 7. 
                 Tree vigor 
                 Moderately vigorous 
               
               
                 8. 
                 Bark color 
                 RHS Grey-Brown 199B 
               
               
                 9. 
                 Leaf shape 
                 Ovate 
               
               
                 10. 
                 Leaf cross-section 
                 Concave 
               
               
                 11. 
                 Leaf blade length 
                 86.1 mm 
               
               
                 12. 
                 Leaf blade width 
                 47.9 mm 
               
               
                 13. 
                 Leaf apex 
                 Acute with weak emargination 
               
               
                 14. 
                 Leaf base 
                 Convex 
               
               
                 15. 
                 Leaf margins 
                 Slightly crenate 
               
               
                 16. 
                 Leaf abaxial color 
                 RHS Green 137C 
               
               
                 17. 
                 Leaf adaxial color 
                 RHS Green 139A 
               
               
                 18. 
                 Petiole length 
                 9.5 mm ± 1.1 
               
               
                 19. 
                 Petiole width 
                 1.5 mm 
               
               
                 20. 
                 Petiole wings 
                 Absent 
               
               
                 21. 
                 Petiole color 
                 RHS Green 137C 
               
               
                 22. 
                 Thorniness 
                 Not present 
               
               
                 23. 
                 Inflorescence type 
                 Clustered 
               
               
                 24. 
                 Flowering habit 
                 Flowers once per year 
               
               
                 25. 
                 Flower size 
                 13.0 mm (medium) 
               
               
                 26. 
                 Flower structure 
                 Complete 
               
               
                 27. 
                 Petal color 
                 RHS White 155C 
               
               
                 28. 
                 Anther color 
                 RHS Yellow 13B 
               
               
                 29. 
                 Pollen viability* 
                 Low (10-20%) 
               
               
                   
               
               
                 (*measured as in-vitro germination) 
               
             
          
         
       
       
         Fruiting, fruit and production characteristics: 
       
     
         [0022]    Fruit of ‘DaisySL’ are slightly obconate in shape with no neck. The fruit has a slightly convex basal end (moderately depressed) with a truncate (slightly depressed) distal end, and a distinctive areola and non-persistent style. The fruit is large-sized for a mandarin (classed as Jumbo by State of California standards and size 21 for industry packing standards) averaging 68.0 mm in diameter and 60.1 mm in height with a very smooth, deep orange rind color (RHS Red-Orange N30C) and slightly conspicuous, slightly raised oil glands. The rind is moderately adherent at maturity and relatively thin averaging 3.0 mm in thickness. This rind thinness is implicated in the tendency of ‘DaisySL’ and its parent ‘Daisy’ to experience a moderately high level of splitting of fruit, sometimes as high as 20% of the total crop. The fruit interior has a fine flesh texture with 10-11 segments and a semi-solid axis of medium size at maturity. The fruit are juicy, averaging approximately 47% juice and 135 g in weight. Fruit from trees on Carrizo and C35 citrange rootstocks average 11.9-12.8% soluble solids and 1.03-1.28% acid in early December at six trial locations in California increasing to 14.2-15.8% soluble solids and 0.78-0.92% acid in early February. The fruit average 2.2 seeds per fruit in the presence of cross-pollination at all locations. Seeds, when present, are polyembryonic, yellow-white in color (Yellow-White 158B) with greyed-yellow (Greyed-Yellow 160C) colyledons and a greyed-yellow (Greyed-Yellow 163B) inner seed coat. 
         [0023]    Full fruit production of ‘DaisySL’ begins in the third year after planting similar to ‘Daisy’. A few fruit will set in the second year after planting but not at commercially acceptable levels. Fruit production on four-year-old trees averaged 27-48 kg at four fruiting trial sites. The original tree at Riverside was similar in fruit production in the fourth year and in years 7, 8 and 9 yielded 77, 32 and 72 kg of fruit respectively indicating that in the earlier years of production the variety has somewhat of a tendency to alternate bear, similar to ‘Daisy’. 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                 Fruit characteristics of ‘DaisySL’ mandarin at maturity 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 1. 
                 Fruit shape 
                 Slightly obconate 
               
               
                 2. 
                 Fruit diameter 
                 68.0 mm ± 4.2 
               
               
                 3. 
                 Fruit height 
                 60.1 mm ± 2.9 
               
               
                 4. 
                 Fruit: shape of basal end 
                 Slightly convex 
               
               
                 5. 
                 Fruit: shape of distal end 
                 Truncate (slightly depressed) 
               
               
                 6. 
                 Fruit: distal end areola 
                 Present (18 mm ± 1.2 mm in diameter) 
               
               
                 7. 
                 Fruit neck 
                 Not present 
               
               
                 8. 
                 Style 
                 Not persistent 
               
               
                 9. 
                 Rind texture 
                 Smooth 
               
               
                 10. 
                 Oil glands 
                 Slightly conspicuous, slightly raised 
               
               
                 11. 
                 Rind Color 
                 RHS Orange-Red N30C 
               
               
                 12. 
                 Rind thickness 
                 3.0 mm 
               
               
                 13. 
                 Albedo thickness 
                 1.5 mm 
               
               
                 14. 
                 Albedo color 
                 RHS Yellow Orange 23C 
               
               
                 15. 
                 Rind adherence 
                 Moderately strong 
               
               
                 16. 
                 Rind separation 
                 Very slight 
               
               
                 17. 
                 Flesh (pulp) color 
                 RHS Orange-Red N30D 
               
               
                 18. 
                 Flesh (pulp) texture 
                 Moderately fine 
               
               
                 19. 
                 Number of segments 
                 10-11 
               
               
                 20. 
                 Axis: structure 
                 Semi-solid 
               
               
                 21. 
                 Axis: size 
                 Medium 
               
               
                 22. 
                 Navel presence 
                 Not present 
               
               
                 23. 
                 # Seeds/fruit (mean) 
                 2.2 (cross-pollinated conditions) 
               
               
                 24. 
                 Seed embryony 
                 Polyembryonic 
               
               
                 25. 
                 Seed coat color 
                 RHS Yellow-White 158B 
               
               
                 26. 
                 Seed cotyledon color 
                 RHS Greyed-Yellow 160C 
               
               
                 27. 
                 Seed inner coat color 
                 RHS Greyed-Yellow 163B 
               
               
                 28. 
                 Fruit weight 
                 135.4 g 
               
               
                 29. 
                 % Juice a   
                 46.8% 
               
               
                 30. 
                 % Soluble solids (at maturity) 
                 14.6% 
               
               
                 31. 
                 % Acid (at maturity) 
                 0.98% 
               
               
                 32. 
                 Season of maturity 
                 Mid-season (early Dec.-January) 
               
               
                 33. 
                 Fruit holding ability 
                 1-2 months 
               
               
                   
                 on tree past maturity 
                   
               
               
                 34. 
                 Fruit quality after storage 
                 Good 
               
               
                   
                 (5.6° C., 30 days) 
               
               
                   
               
               
                   a weight of juice extracted with a reamer as a percentage of fruit weight 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Crop yields for ‘DaisySL’ and ‘Daisy’ 
               
               
                 (control trees) at three trial sites over 
               
               
                 two years, 2006/2007 and 2007/2008. 
               
             
          
           
               
                   
                   
                   
                   
                   
                 2006/ 
                 2006/ 
                 2007/ 
                 2007/ 
               
               
                   
                   
                   
                   
                   
                 7 
                 7 
                 8 
                 8 
               
               
                   
                   
                   
                 Tree 
                   
                 Mean 
                 Yield 
                 Mean 
                 Yield 
               
               
                   
                   
                   
                 Age 
                   
                 Yield 
                 Range 
                 Yield 
                 Range 
               
               
                   
                   
                 # 
                 2008 
                 Root- 
                 (kg/ 
                 (kg/ 
                 (kg/ 
                 (kg/ 
               
               
                 Site 
                 Selection 
                 Trees 
                 (yrs) 
                 stock 
                 tree) 
                 tree) 
                 tree) 
                 tree) 
               
               
                   
               
               
                 River- 
                 ‘DaisySL’ 
                 10 
                 6.0 
                 Car- 
                 32.8 
                 24.3- 
                 53.8 
                 43.3- 
               
               
                 side 
                   
                   
                   
                 rizo 
                   
                 44.9 
                   
                 65.4 
               
               
                 River- 
                 ‘DaisySL’ 
                 10 
                 6.0 
                 C35 
                 37.6 
                 27.8- 
                 54.9 
                 47.0- 
               
               
                 side 
                   
                   
                   
                   
                   
                 48.9 
                   
                 61.4 
               
               
                 River- 
                 ‘DaisySL’ 
                  1 
                 9.0 
                 Car- 
                 77.1 
                 77.1 
                 32.1 
                 32.1 
               
               
                 side 
                 (mother) 
                   
                   
                 rizo 
                   
                   
                   
                   
               
               
                 River- 
                 ‘Daisy’ 
                  2 
                 9.0 
                 Car- 
                 70.6 
                 67.5- 
                 39.4 
                 35.2- 
               
               
                 side 
                 control 
                   
                   
                 rizo 
                   
                 73.6 
                   
                 43.6 
               
               
                 Santa 
                 ‘DaisySL’ 
                  5 
                 4.0 
                 Car- 
                 26.4 
                 19.8- 
                 42.6 
                 34.6- 
               
               
                 Paula 
                   
                   
                   
                 rizo 
                   
                 38.4 
                   
                 55.7 
               
               
                 Santa 
                 ‘DaisySL’ 
                  5 
                 4.0 
                 C35 
                 28.9 
                 22.6- 
                 44.0 
                 37.1- 
               
               
                 Paula 
                   
                   
                   
                   
                   
                 34.9 
                   
                 56.4 
               
               
                 Santa 
                 ‘Daisy’ 
                  2 
                 4.0 
                 Car- 
                 26.4 
                 24.9- 
                 47.9 
                 45.1- 
               
               
                 Paula 
                 control 
                   
                   
                 rizo 
                   
                 27.9 
                   
                 50.7 
               
               
                 Irvine 
                 ‘DaisySL’ 
                  9 
                 4.0 
                 Car- 
                 31.4 
                 24.5- 
                 45.9 
                 38.9- 
               
               
                   
                   
                   
                   
                 rizo 
                   
                 39.7 
                   
                 54.8 
               
               
                 Irvine 
                 ‘DaisySL’ 
                 10 
                 4.0 
                 C35 
                 33.6 
                 27.4- 
                 47.6 
                 33.1- 
               
               
                   
                   
                   
                   
                   
                   
                 40.0 
                   
                 57.6 
               
               
                 Irvine 
                 ‘Daisy’ 
                  2 
                 4.0 
                 Car- 
                 28.9 
                 27.0- 
                 50.1 
                 44.0- 
               
               
                   
                 control 
                   
                   
                 rizo 
                   
                 30.8 
                   
                 56.2 
               
               
                   
               
             
          
         
       
     
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                 TABLE 4 
               
             
             
               
                   
               
               
                 Seed counts (average number of seeds per fruit) for 
               
               
                 ‘DaisySL’ and ‘Daisy’ (control trees) at four trial 
               
               
                 sites over two years, 2006/2007 and 2007/2008. 
               
             
          
           
               
                   
                   
                   
                   
                 2006/7 
                 2007/8 
                 % 
               
               
                   
                   
                 Tree 
                   
                 Mean 
                 Mean 
                 Fruit 
               
               
                   
                   
                 Age 
                   
                 Seeds/ 
                 Seeds/ 
                 with 
               
               
                   
                   
                 (yrs) 
                 Root- 
                 Fruit 
                 Fruit 
                 0-2 
               
               
                 Site 
                 Selection 
                 2008 
                 stock 
                 (range) 
                 (range) 
                 seeds 
               
               
                   
               
               
                 Riverside 
                 ‘DaisySL’ 
                  6.0 
                 Carrizo 
                  2.41 
                  1.41 
                 81.3 
               
               
                   
                   
                   
                   
                 (1.88-3.30) 
                 (0.88-2.30) 
                   
               
               
                 Riverside 
                 ‘DaisySL’ 
                  6.0 
                 C35 
                  2.19 
                  1.39 
                 83.8 
               
               
                   
                   
                   
                   
                 (1.50-2.88) 
                 (1.02-2.09) 
                   
               
               
                 Riverside 
                 ‘DaisySL’ 
                  9.0 
                 Carrizo 
                  2.26 
                  1.86 
                 78.9 
               
               
                   
                 (mother) 
                   
                   
                   
                   
                   
               
               
                 Riverside 
                 ‘Daisy’ 
                  9.0 
                 Carrizo 
                 18.9  
                 17.2  
                  3.2 
               
               
                   
                 control 
                   
                   
                   
                   
                   
               
               
                 Santa Paula 
                 ‘DaisySL’ 
                  4.0 
                 Carrizo 
                  1.21 
                  1.09 
                 89.7 
               
               
                   
                   
                   
                   
                 (0.78-2.31) 
                 (0.69-1.97) 
                   
               
               
                 Santa Paula 
                 ‘DaisySL’ 
                  4.0 
                 C35 
                  1.49 
                  1.26 
                 86.5 
               
               
                   
                   
                   
                   
                 (1.06-2.72) 
                 (0.90-2.22) 
                   
               
               
                 Santa Paula 
                 ‘Daisy’ 
                  4.0 
                 Carrizo 
                 15.3  
                 14.8  
                  5.1 
               
               
                   
                 control 
                   
                   
                   
                   
                   
               
               
                 Irvine 
                 ‘DaisySL’ 
                  4.0 
                 Carrizo 
                  2.16 
                  1.61 
                 79.9 
               
               
                   
                   
                   
                   
                 (1.88-2.76) 
                 (1.15-2.32) 
                   
               
               
                 Irvine 
                 ‘DaisySL’ 
                  4.0 
                 C35 
                  2.13 
                  1.43 
                 81.4 
               
               
                   
                   
                   
                   
                 (1.51-2.87) 
                 (0.96-2.19) 
                   
               
               
                 Irvine 
                 ‘Daisy’ 
                  4.0 
                 Carrizo 
                 17.4  
                 14.9  
                  6.1 
               
               
                   
                 control 
                   
                   
                   
                   
                   
               
               
                 Lindcove 
                 ‘DaisySL’ 
                  4.0 
                 Carrizo 
                  2.22 
                  1.44 
                 86.5 
               
               
                   
                   
                   
                   
                 (1.35-3.02) 
                 (0.77-2.11) 
                   
               
               
                 Lindcove 
                 ‘DaisySL’ 
                  4.0 
                 C35 
                  2.33 
                  1.36 
                 88.4 
               
               
                   
                   
                   
                   
                 (1.45-2.95) 
                 (0.88-1.97) 
                   
               
               
                 Lindcove 
                 ‘Daisy’ 
                 15   
                 Carrizo 
                 17.1  
                 16.3  
                  3.1 
               
               
                   
                 control 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
               
                   
               
             
             
               
                 Mean and standard deviation (S.D.) of 
               
               
                 soluble solids, acid and solids/acid ratio for 
               
               
                 DaisySL at four trial sites, 2007/8 crop year. 
               
             
          
           
               
                   
                   
                 Soluble 
                   
                 Soluble 
                   
                   
                   
               
               
                   
                   
                 Solids 
                   
                 Solids 
                   
                 % 
                   
               
               
                   
                   
                 % 
                   
                 % 
                   
                 Acid 
                   
               
               
                 Site 
                 Date 
                 Carrizo 
                 S.D. 
                 C35 
                 S.D. 
                 Carrizo 
                 S.D. 
               
               
                   
               
               
                 River- 
                 Dec. 6, 
                 12.8 
                 0.29 
                 12.6 
                 0.48 
                 1.28 
                 0.18 
               
               
                 side 
                 2007 
                   
                   
                   
                   
                   
                   
               
               
                 River- 
                 Jan. 9, 
                 14.2 
                 0.38 
                 14.0 
                 0.39 
                 1.00 
                 0.12 
               
               
                 side 
                 2008 
                   
                   
                   
                   
                   
                   
               
               
                 River- 
                 Feb. 6, 
                 15.8 
                 0.43 
                 15.6 
                 0.34 
                 0.88 
                 0.10 
               
               
                 side 
                 2005 
                   
                   
                   
                   
                   
                   
               
               
                 Santa 
                 Dec. 5, 
                 11.9 
                 0.39 
                 12.0 
                 0.26 
                 0.97 
                 0.19 
               
               
                 Paula 
                 2007 
                   
                   
                   
                   
                   
                   
               
               
                 Santa 
                 Jan. 11, 
                 13.1 
                 0.19 
                 13.7 
                 0.39 
                 0.90 
                 0.09 
               
               
                 Paula 
                 2008 
                   
                   
                   
                   
                   
                   
               
               
                 Santa 
                 Feb. 8, 
                 14.7 
                 0.33 
                 14.2 
                 0.44 
                 0.80 
                 0.16 
               
               
                 Paula 
                 2008 
                   
                   
                   
                   
                   
                   
               
               
                 Irvine 
                 Dec. 7, 
                 12.1 
                 0.66 
                 11.9 
                 0.49 
                 1.03 
                 0.14 
               
               
                   
                 2007 
                   
                   
                   
                   
                   
                   
               
               
                 Irvine 
                 Jan. 7, 
                 13.9 
                 0.38 
                 12.8 
                 0.55 
                 0.89 
                 0.13 
               
               
                   
                 2008 
                   
                   
                   
                   
                   
                   
               
               
                 Irvine 
                 Feb. 6, 
                 15.3 
                 0.44 
                 13.5 
                 0.26 
                 0.78 
                 0.08 
               
               
                   
                 2008 
                   
                   
                   
                   
                   
                   
               
               
                 Lind- 
                 Dec. 12, 
                 12.1 
                 0.22 
                 12.0 
                 NA 
                 1.33 
                 0.24 
               
               
                 cove 
                 2007 
                   
                   
                   
                   
                   
                   
               
               
                 Lind- 
                 Jan. 15, 
                 13.0 
                 0.26 
                 13.3 
                 0.52 
                 1.00 
                 0.11 
               
               
                 cove 
                 2008 
                   
                   
                   
                   
                   
                   
               
               
                 Lind- 
                 Feb. 12, 
                 15.8 
                 0.33 
                 15.5 
                 0.41 
                 0.90 
                 0.07 
               
               
                 cove 
                 2008 
               
               
                   
               
             
          
           
               
                   
                 % 
                   
                 S/A 
                 S/A 
               
               
                   
                 Acid 
                   
                 Ratio 
                 Ratio 
               
             
          
           
               
                   
                 Site 
                 Date 
                 C35 
                 S.D. 
                 Carrizo 
                 C35 
               
               
                   
               
               
                   
                 River- 
                 Dec. 6, 
                 1.22 
                 0.11 
                 10.0 
                  9.8 
               
               
                   
                 side 
                 2007 
                   
                   
                   
                   
               
               
                   
                 River- 
                 Jan. 9, 
                 0.95 
                 0.10 
                 14.2 
                 14.7 
               
               
                   
                 side 
                 2008 
                   
                   
                   
                   
               
               
                   
                 River- 
                 Feb. 6, 
                 0.92 
                 0.06 
                 17.9 
                 17.0 
               
               
                   
                 side 
                 2005 
                   
                   
                   
                   
               
               
                   
                 Santa 
                 Dec. 5, 
                 0.89 
                 0.07 
                 13.5 
                 14.9 
               
               
                   
                 Paula 
                 2007 
                   
                   
                   
                   
               
               
                   
                 Santa 
                 Jan. 11, 
                 0.84 
                 0.07 
                 14.7 
                 16.3 
               
               
                   
                 Paula 
                 2008 
                   
                   
                   
                   
               
               
                   
                 Santa 
                 Feb. 8, 
                 0.81 
                 0.06 
                 17.3 
                 17.5 
               
               
                   
                 Paula 
                 2008 
                   
                   
                   
                   
               
               
                   
                 Irvine 
                 Dec. 7, 
                 1.10 
                 0.12 
                 11.7 
                 10.8 
               
               
                   
                   
                 2007 
                   
                   
                   
                   
               
               
                   
                 Irvine 
                 Jan. 7, 
                 0.90 
                 0.14 
                 14.5 
                 14.2 
               
               
                   
                   
                 2008 
                   
                   
                   
                   
               
               
                   
                 Irvine 
                 Feb. 6, 
                 0.77 
                 0.11 
                 17.8 
                 17.5 
               
               
                   
                   
                 2008 
                   
                   
                   
                   
               
               
                   
                 Lind- 
                 Dec. 12, 
                 1.28 
                 0.19 
                  9.1 
                  9.4 
               
               
                   
                 cove 
                 2007 
                   
                   
                   
                   
               
               
                   
                 Lind- 
                 Jan. 15, 
                 1.02 
                 0.06 
                 13.0 
                 13.0 
               
               
                   
                 cove 
                 2008 
                   
                   
                   
                   
               
               
                   
                 Lind- 
                 Feb. 12, 
                 0.88 
                 0.08 
                 17.6 
                 17.6 
               
               
                   
                 cove 
                 2008 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Comparison of ‘DaisySL’ with other 
               
               
                 late season, low-seeded mandarins. 
               
               
                 Data for Riverside, California. 
               
             
          
           
               
                   
                   
                   
                   
                   
                 Gold 
                   
               
               
                 Trait 
                 ‘DaisySL’ 
                 TDE2 
                 TDE3 
                 TDE4 
                 Nugget 
                 Tango 
               
               
                   
               
               
                 Matu- 
                 Early 
                 Febru- 
                 January- 
                 Febru- 
                 Febru- 
                 Febru- 
               
               
                 rity 
                 Decem- 
                 ary 
                 February 
                 ary 
                 ary- 
                 ary- 
               
               
                   
                 ber- 
                   
                   
                   
                 March 
                 March 
               
               
                   
                 early 
                   
                   
                   
                   
                   
               
               
                   
                 January 
                   
                   
                   
                   
                   
               
               
                 Seeds 
                  2.2 
                  0.02 
                  0.29 
                  0.32 
                 &lt;0.1 
                  0.22 
               
               
                 per 
                   
                   
                   
                   
                   
                   
               
               
                 fruit 
                   
                   
                   
                   
                   
                   
               
               
                 RHS 
                 Orange- 
                 Orange- 
                 Orange- 
                 Orange- 
                 Orange 
                 Orange 
               
               
                 rind 
                 Red 
                 Red 
                 Red 
                 Red 
                 25A 
                 N25A 
               
               
                 color 
                 N30C 
                 N3OD 
                 N30C 
                 N30C 
                   
                   
               
               
                 Rind 
                 very 
                 slightly 
                 papillate 
                 smooth 
                 bumpy 
                 smooth 
               
               
                 texture 
                 smooth 
                 pitted 
                   
                   
                   
                   
               
               
                 Fruit 
                 135   
                 185   
                 134   
                 175   
                 108   
                 90   
               
               
                 weight 
                   
                   
                   
                   
                   
                   
               
               
                 (g) 
                   
                   
                   
                   
                   
                   
               
               
                 Fruit 
                  0.88 
                  0.78 
                  0.85 
                  0.78 
                  0.88 
                  0.81 
               
               
                 height/ 
                   
                   
                   
                   
                   
                   
               
               
                 width 
                   
                   
                   
                   
                   
                   
               
               
                 Alter- 
                 medium 
                 medium 
                 medium- 
                 medium- 
                 high 
                 medium 
               
               
                 nate 
                   
                   
                 high 
                 high 
                   
                   
               
               
                 bear- 
                   
                   
                   
                   
                   
                   
               
               
                 ing 
               
               
                   
               
             
          
         
       
     
         [0024]    Fruit storage trials included storage of washed but not waxed fruit at 5.6° C. for up to 60 days with fruit samples taken every 14 days for analysis. Data indicate that the storage characteristics of ‘DaisySL’ are fair with some rind deterioration (rind drying) and some significant indication of fungal disease problems in 23% of the fruit. There was no significant deterioration in juice quality or taste over a 30 day storage period in those fruit without fungal pathogens, but with more significant rind deterioration if kept to 60 days. Overall Daisy can only be considered to be fair in storage ability due primarily to the somewhat susceptible nature of the rind to pathogen organisms. Fungicide treatment and waxing might decrease decay and rind deterioration during storage. 
         [0025]    No susceptibilities to plant or fruit diseases or to pests beyond those normally associated with citrus species have been observed.