Abstract:
Daunorubicin (also known as daunomycin) derivatives of the formula ##STR1## wherein X is ##STR2## and R is --COCF 3  or H which are prepared by hydrogenating 9-desacetyl-9-keto-N-trifluoroacetyldaunorubicin, an unexpectedly stable key-intermediate which is itself a new compound, are useful in treating certain mammalian tumors.

Description:
The invention described herein was made in the course of work performed under a grant from the United States Department of Health, Education and Welfare. 
    
    
     CROSS REFERENCE TO RELATED APPLICATIONS 
     None. 
     BACKGROUND OF THE INVENTION 
     Field of the Invention 
     The invention relates to glycoside antibiotics, their preparation and use. 
     The Prior Art 
     The glycoside antibiotics daunorubicin (also known as daunomycin) and doxorubicin (also known as adriamycin) are respectively described and claimed in British Patents 1,003,383 and 1,161,278, both of which are owned by the unrecorded assignee hereof. 
     SUMMARY OF THE INVENTION 
     The present invention provides, in one aspect thereof, a new class of daunorubicin derivatives which are useful in treating certain mammalian tumors. These new derivatives are compounds of the formula I ##STR3## wherein X is ##STR4## and R is --COCF 3  or H 
     The invention also provides a process of preparing these derivatives. The process proceeds through a novel intermediate, 9-desacetyl-9-keto-N-trifluoroacetyldaunorubicin, which also forms part of the invention. This intermediate is an unexpectedly stable compound, which, when hydrogenated with an alkali metal borohydride leads to the compounds of formula I. According to the process of the invention which is schematically shown below, daunorubicin (II) is converted into 9-desacetyl-9-keto-N-trifluoroacetyldaunorubicin (V). ##STR5## 
     In more detail, as shown above, the carbonyl group at the 13-position of daunorubicin (II) is reduced to the corresponding alcohol (III) with sodium borohydride. The reaction is carried out in water at room temperature to give (III), isolated as the hydrochloride, in about 80% yield. The amino group in the sugar residue of the resulting alcohol (III) is protected with an N-trifluoroacetyl group which is subsequently removed under mild conditions that do not affect the remaining portion of the anthracycline molecule. The N-trifluoroacetylation is performed by treatment with trifluoroacetic anhydride, followed by hydrolysis of the O-trifluoroacetyl group with methanol to form a protected derivative (IV) in 74% yield. The oxidation of (IV) is carried out in t-butyl alcohol in the presence of two equivalents of sodium periodate at room temperature for two hours. The protected intermediate (IV), which is insoluble in the reaction mixture, was obtained in about 50% yield. The compound (IV), by treatment with sodium borohydride-cyanide (NaBH 3  CN) under acid conditions, is converted into an epimeric mixture of (IA) and (IB) in an approximate ratio of 8:1. The separation of these compounds by chromatography on a column of silicic acid, followed by mild alkaline treatment to remove the N-trifluoroacetyl protective group, gives 9-desacetyl-daunorubicin (IC) and 9-desacetyl-9-epi-daunorubicin (ID) both isolated as the hydrochlorides. 
     The new compounds (IC) and (ID) both display antimitotic activity and are useful therapeutic agents for the treatment of mammalian tumors. 
    
    
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     The invention will now be illustrated by the following Examples. 
     EXAMPLE 1 
     N-trifluoroacetyl-13-dihydrodaunorubicin (IV) 
     A solution of 3.0 g. of daunorubicin hydrochloride (II) in 300 ml. of water was adjusted to pH 9.5 with aqueous 0.1 N sodium hydroxide and treated with 0.3 g. of sodium borohydride at room temperature for seven minutes. The reaction mixture was poured into 750 ml. of aqueous 0.25 N hydrochloric acid with vigorous stirring to eliminate the excess reducing agent (NaBH 4 ). The solution was then adjusted to pH 8.6 and extracted with chloroform until the extracts were no longer colored. The chloroform extracts were combined, dried over anhydrous sodium sulphate, and evaporated under vacuum to 50 ml. The resulting red solution, after being adjusted to pH 3.5 (Congo Red) with anhydrous methanolic hydrogen chloride, was mixed with excess diethyl ether to give 2.8 g. of pure 13-dihydrodaunorubicin (III) as the hydrochloride. 
     A suspension of the 2.8 g. of 13-dihydrodaunorubicin hydrochloride (III) in 300 ml. of chloroform was treated with 20 ml. of trifluoroacetic anhydride at 0° for 1 hour. The reaction mixture was evaporated to a residue under vacuum. The residue was dissolved in 200 ml. of methanol and neutralized with an aqueous saturated solution of sodium bicarbonate. After 30 minutes at room temperature, the solvent was eliminated under vacuum and the aqueous solution was extracted with chloroform until the extracts were no longer colored. The chloroform extracts were combined, washed with water, dried over anhydrous sodium sulphate, evaporated to 30 ml., and then mixed with excess petroleum ether to give 2.3 g. of pure N-trifluoroacetyl-13-dihydrodaunorubicin (IV); m.p. 164°-166° C. (dec.); TLC (Thim Layer Chromatography) on Merck Kieselgel 60 F 254  using a chloroform-acetone solvent system (2:1 v/v) = Rf 0.25. 
     EXAMPLE 2 
     9-Desacetyl-9-keto-N-trifluoroacetyldaunorubicin (V) 
     A solution of 2.4 g. of N-trifluoroacetyl-13-dihydrodaunorubicin (IV) in 120 ml. of t-butyl alcohol was treated with 1.6 g. of sodium periodate dissolved in 120 ml. of water. The reaction mixture was stirred at room temperature for two hours. The resulting precipitate (V) was filtered off, washed with water and dried under vacuum to yield 1.4 g. of pure 9-desacetyl-9-keto-N-trifluoroacetyldaunorubicin (V) were obtained; m.p. 200° C. (dec.); TLC on Merck Kieselgel 60 F 254  using a chloroform-acetone solvent system (2:1 v/v) = Rf 0.57. 
     
         ______________________________________Elementalanalysis:    calcd. % for C.sub.27 H.sub.24 F.sub.3 NO.sub.10 :                       H 4.18; C 55.9    found %:           H 4.26; C 55.6______________________________________ 
    
     EXAMPLE 3 
     9-Desacetyl-daunorubicin (IC) and 9-desacetyl-9-epi-daunorubicin (ID) 
     A solution of 1.7 g. of 9-desacetyl-9-keto-N-trifluoroacetyldaunorubicin (V) in 250 ml. of dioxane and 50 ml. of water was adjusted to pH 3 with aqueous 1 N hydrochloric acid and treated with 1 g. of sodium borohydride cyanide (NaBH 3  CN) at room temperature for 24 hours, the acid condition being maintained by the addition of 1 N hydrochloric acid. The reaction mixture was mixed with water (300 ml.) and extracted with chloroform (5 × 200 ml.). The organic phase (combined CHCl 3  extracts) was washed with water, dried over anhydrous sodium sulphate, and evaporated to a residue under vacuum. The residue (1 g.), containing N-trifluoroacetyl-9-desacetyldaunorubicin (IA) and N-trifluoroacetyl-9-desacetyl-9-epi-daunorubicin (IB) was chromatographed on a column of silica gel using chloroform with increasing amounts of acetone as the eluent to give 0.85 g. of pure N-trifluoroacetyl-9-desacetyldaunorubicin (IA); m.p. 204°-206° C. (dec.); TLC on Merck Kieselgel 60 F 254  using a chloroform-acetone solvent system (2:1 v/v) = Rf : 0.44 and 0.1 g. of pure N-trifluoroacetyl-9-desacetyl-9-epi-daunorubicin (IB); m.p. 108°-182° C. (dec.); Rf : 0.3 using the same solvent system. In order to hydrolyze off the N-trifluoroacetyl groups, the products were treated as follows: 50 ml. of 0.1 N sodium hydroxide were added, after 30 minutes at 0° C.; the pH was adjusted to 8.4 with 0.1 N hydrochloric acid and the solution was repeatedly extracted with chloroform. The combined chloroform extracts were dried over anhydrous sodium sulphate and evaporated under vacuum to 20 ml. volume. The concentrated solution, upon addition of the stoichiometric amount of methanolic hydrogen chloride and diethyl ether, gave a red precipitate which was collected, washed with diethyl ether and dried under vacuum. 9-Desacetyldaunorubicin (IC) had a m.p. of 166°-167° C. (dec.); [α] D   25  = + 282° (c = 0.15 in methanol); TLC on Kieselgel plates F 254  (Merck) with a chloroform:methanol:water solvent system (13:6:1 v/v) = Rf 0.55. 
     
         ______________________________________              Elemental Analysis:calcd. % for C.sub.25 H.sub.27 NO.sub.9 HCl:              H 5.42; C 57.52; N 2.68found:             H 5.45; C 57.16; N 2.42______________________________________ 
    
     9-desacetyl-9-epi-daunorubicin (ID) had a m.p. of 176° C. (dec.); Rf = 0.4 using the same solvent system. 
     BIOLOGICAL ACTIVITY 
     Table 1 
     The activity of doxorubicin (also known as adriamycin), 9-desacetyldaunorubicin (IC) and 9-desacetyl-9-epi-daunorubicin (ID) were tested against P 388 lymphocytic leukemia in C.D.F. male mice (tumor inoculum 10 6  cells; i.p.). Treatment with drug: i.p. on days 1 to 9. 
     
         ______________________________________Compound          Dose mg/kg   T/C.sup.a______________________________________Doxorubicin       4            83             2            180             1            171             0.5          142             0.25         1529-desacetyldaunorubicin             25           73(IC)              12.5         228             6.25         180             3.13         174             1.56         1559-desacetyl-9-epi-             25           66daunorubicin      12.5         66(ID)              6.25         171             3.13         157             1.56         142daunorubicin      4            88             2            126             1            171             0.5          150             0.2          155______________________________________ .sup.a Median survival time expressed as percent of untreated controls. 
    
     Table 2 
     The activity of doxorubicin and 9-desacetyldaunorubicin (IC) were tested against P 388 lymphocytic leukemia in CDF 1  male mice (tumor inoculum 10 6  cells; i.p.). Treatment with drug:i.p. on days 5, 9 and 13. 
     
         ______________________________________Compound          Dose mg/kg   T/C.sup.a______________________________________Doxorubicin       16           120             8            163             4            136             2            125             1            125Daunorubicin      16           127             8            111             4            103             2            107             1            989-desacetyldaunorubicin             113          116(IC)              75           181             37.5         136             18.8         145             9.4          133             4.7          109______________________________________ .sup.a Median survival time expressed as percent of untreated controls. 
    
     The data in Tables 1 and 2 show that the compounds of the invention are considerably more effective and less toxic than doxorubicin. 
     Modifications and variations can, of course, be made without departing from the spirit and scope of the invention.