Abstract:
A boat holding tank for anesthetizing fish is described having a pulsator, with the pulsator having an anode output and a cathode output; a holding tank capable of receiving an aquatic animal, the holding tank further comprising an anode probe and a cathode probe, with the anode cathode probe of the holding tank switchably connected to the anode and cathode output of the pulsator, such that when the pulsator is activated the aquatic animal in the holding tank is anesthetized.

Description:
RELATED APPLICATIONS 
       [0001]    This application claims the benefit of U.S. Provisional Application No. 61/168,476, filed on Apr. 10, 2009, the contents herein incorporated into this application by reference. 
     
    
     BACKGROUND 
       [0002]    The present inventive subject matter relates to holding tanks for the stunning and/or euthanization of fish using electric currents on boats. 
         [0003]    The effect of electric currents on fish are well known in the prior art and especially in the general techniques of electrofishing. Electrofishing involves the use of electric currents to attract and/or repel fish with the intent of creating aquatic barriers, sample fish barriers, and/or to increase collection yields. 
         [0004]    It has been established that relatively small potentials that are impressed across the body of a fish invoke a flight reaction. Larger potentials result in the alignment of the fish with the electric current, or electrotaxis. Still larger potentials may result in unconsciousness or complete euthanasia of the fish. 
         [0005]    Electrofishing has traditionally been used in freshwater lakes and streams and is the subject of U.S. Pat. Nos. 5,445,111; 5,327,854; 4,750,451; 4,672,967; 4,713,315; 5,111,379; 5,233,782; 5,270,912; 5,305,711; 5,311,694; 5,327,668; 5,341,764; 5,551,377; and 6,978,734 which are incorporated herein by reference. 
         [0006]    A recurring problem with the examination of laboratory fish is that they tend to be very active. The small size of the fish combined with their activity can impair the researcher from making precise scientific measurements unless the fish is caught and inspected. Thus direct examination of fishes is preferred to “in situ” measurements. 
         [0007]    An aspect of fish conservation involves the collection, tagging, and then the subsequent release of tagged fish. If the tagged fish is then captured at a later date, the difference in the fishes&#39; location, health, and size can be compared to when the fish was first caught. Alternately, a group of fish may be caught from a particular area to determine the statistical distribution of each of the fish species. Furthermore, fishes may be caught to determine if a fish is from wild stock or from fishery stock. In all of these cases, when the fish are captured they are typically placed in a holding tank on the boat, and then they are inspected for species and/or tag information, and then returned to the water. When the fish is on the boat it may be in an excited or agitated state due to having been just caught. The agitated state of the fish will typically make it difficult for fish scientists to handle the fish. An agitated fish in the holding tank may also injure other fishes in the same tank. The scientists may also have to accommodate inclement weather conditions on the boat that may include pitching and rolling of the deck. 
         [0008]    What is desired is a safe way to anesthetizing fish in the holding tank of a boat as to reduce the amount of stress on the fish while they are inspected and handled. Prior art solutions and techniques to induce anesthesia in fish involve the addition of chemicals to the tank. See U.S. Pat. Nos. 3,551, 566; 3,644,625; and 4,807,615; which are incorporated by reference. Chemicals used for anesthesia are expensive to acquire, pose a storage and maintenance problem, and are at risk of degradation. Also the rocking motion of the boat makes dispensing the chemicals into the boat holding tank a difficult process. 
         [0009]    Furthermore, there may be environmental restrictions on the discharge of the holding tank contents once a chemical anesthetic in introduced into the holding tank water. If there are restrictions on the discharge of water, this requires that the holding tank water be stored and removed upon the return to dock. The process of storing holding tank water increases the operational cost of the boat due to the increased energy costs. 
         [0010]    Therefore, what is desired is an apparatus to immobilize fish and place the fish in an anesthesia state while in a holding tank in the boat. It is also desired that the apparatus pose little or no attendant risk to the researchers or fisherman whom are close to the holding tank. It is also desired that this apparatus can operate without significant modification to the boat infrastructure. It is also desired that this apparatus operate without the use of chemical additives. 
       SUMMARY 
       [0011]    The present inventive subject matter overcomes problems in the prior art by providing a boat holding tank for anesthetizing fish with a pulsator, the pulsator having an anode output and a cathode output; a holding tank capable of receiving an aquatic animal, the holding tank further comprising an anode probe and a cathode probe, the anode probe switchably connected to the anode output and the cathode probe switchably connected to the cathode output; such that when the pulsator is activated and the anode and cathode outputs from the pulsator are switchably connected to the anode and cathode probes in the holding tank, the aquatic animal is anesthetized. Also described is a boat holding tank for anesthetizing fish has a voltage waveform that is direct current. The boat holding tank for anesthetizing fish that has the voltage waveform is alternating current. Also described is a boat holding tank for anesthetizing fish that has the voltage waveform is pulsed direct current. Also described is a boat holding tank for anesthetizing fish that has the pulsator with an electrofishing anode and cathode, the electrofishing anode and cathode capable of being electrically connected to a pulsator anode and cathode; and having a control input, a control input capable of selecting the electrical path of the anode output and cathode, such that when the control input is activated, the anode and cathode probe pair are connected to the anode and cathode probes in the holding tank, and such that when the control input is not activated, the anode and cathode probe pair are connected to the electrofishing anode and cathode. Also described is a boat holding tank for anesthetizing fish as having a pulsator that creates a potential difference between the first electrode and the second electrode creates where the potential difference induces euthanasia in an aquatic animal. Also described is a boat holding tank for anesthetizing fish as where the pulsator has: a pressure mat, the pressure mat proximate to the boat holding tank, an electrical switch, and the electrical switch is activated when a person steps on the pressure mat; such that when the electrical switch is activated, the pulsator is deactivated. 
         [0012]    The present inventive subject matter includes a method for inducing electroanesthesia in aquatic animals located in a boat holding tank, the method having the steps of connecting an anode output and a cathode output from the pulsator to the anode probe and the cathode probe in the holding tank; energizing the pulsator for a time period, the energization of the pulsator creating a potential difference between the anode probe and the cathode probe; such that the time period ends when the aquatic animal is anesthetized. Also described is a method for inducing electroanesthesia so that the potential difference between the anode probe and the cathode probe is an alternating current waveform. Also described is a method for inducing electroanesthesia so that the potential difference between the anode probe and the cathode probe is a direct current waveform. Also described is a method for inducing electroanesthesia so that the potential difference between the anode probe and the cathode probe is a pulsed direct current waveform. Also described is a method for inducing electroanesthesia which includes the steps of disabling the pulsator if a person is on a pressure mat proximate to the holding tank. Also described is a method for inducing electroanesthesia which includes the steps of disabling the pulsator if a person is on a pressure mat proximate to the holding tank. Also described is a method for inducing electroanesthesia which includes the steps of selecting the anode and cathode output from the pulsator to be directed to the electrofisher anode and cathode. 
         [0013]    These and other embodiments are described in more detail in the following detailed descriptions and the figures. 
         [0014]    The foregoing is not intended to be an exhaustive list of embodiments and features of the present inventive subject matter. Persons skilled in the art are capable of appreciating other embodiments and features from the following detailed description in conjunction with the drawings. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0015]      FIG. 1  is a top view diagram of a eletrofishing boat with a holding tank, an examination table, and electrofishing equipment. 
           [0016]      FIG. 2  is a close up view of the improved holding tank incorporating an anode and cathode leads connected to the pulsator. 
           [0017]      FIG. 3  is a systems diagram of the pulsator switch between the electrofishing anode and cathode, and the electroanesthesia anode and cathode. 
           [0018]      FIG. 4  is flowchart for the switching power between the electrofishing anode and cathode and the electroanesthesia anode and cathode. 
           [0019]      FIG. 5  is a view of the holding tank with a switch mat surrounding the holding tank. 
       
    
    
     LIST OF REFERENCE CHARACTERS 
       [0000]    
       
           100 —Prior Art Electrofishing Boat 
           110 ,  110 ′,  110 ″—Fish 
           120 —Net 
           130 —Holding Tank 
           140 —Pulsator 
           142 —Electrofishing Cathode 
           144 —Electrofishing Anode 
           150 —Inspection Table 
           210 —Boat Deck 
           242 —Electroanethesia (EA) Cathode Electrode 
           244 —Electroanesthesia (EA) Anode Electrode 
           300 —Pulsator Switch System Diagram 
           310 —DPDT Switch 
           320 —DPDT Switch Control 
           330 —Control Input 
           400 —Pulsator Output Flowchart 
           410 —Start 
           420 —Check if electroanesthesia switch is on. 
           430 —Select electroanesthia power setting 
           440 —Select Electrofishing Power Setting 
           510 —Pressure Mat 
           520 —Pressure Mat Output 
           530 —Hand 
       
     
       DETAILED DESCRIPTION 
       [0043]    Representative embodiments according to the inventive subject matter are shown in  FIGS. 1-5  wherein similar features share common reference numerals. 
         [0044]    The term “fish” refers to fish as typically caught by research vessels in saltwater and freshwater environments, but, is not limited to a particular species. The term “fish” should also include the class of mammals that live or frequent waters, for example, seals, dolphins, manatees, and/or pinnipeds. 
         [0045]    The term “holding tank” is generally a 30-200 gallon tank that is used by researchers and is customarily bolted to or set on the deck of a boat. Holding tanks can also be larger or smaller (e.g. aquarium size tanks). Holding tanks will generally be found on the deck of the boat, but may also be below the deck of the boat. 
         [0046]    The term “pulsator” refers to an electrical apparatus, generally an electrical generator used for electrofishing and/or electroanesthesia. Such devices include products manufactured by Smith-Root, Inc. (Vancouver, Wash., USA), with representative models being the Type VI-A Electrofisher (the users manual incorporated herein by reference and attached as Appendix A) and the Portable Electoanesthesia System (PES). 
         [0047]    Now referring to  FIG. 1 , which depicts a prior art electrofishing boat  100  that is located in the water proximate to fish  110 . The operator on the electrofishing boat  100  uses a net  120  to capture fish  110  in the water. The fish  110  are then transported into the holding tank  130  for further examination on a table  150 , typically for research purposes. To aid in attracting fish to an area proximate to the net  120 , the operator of the electrofishing boat  100  will energize a pulsator  140 , which creates a potential between the cathode  142  and the anode  144 . The potential difference between the cathode  142  and the anode  144  creates a current flow between the cathode  142  and the anode  144 . The current flow creates a potential difference across the body of a fish located within the field between the cathode  142  and the anode  144 . At a certain electrical potential, the fish  110  are attracted to the electrode. The system  100  has a water tank  110 , two electrodes  120 ′,  120 ″ immersed in the water tank  110 , two wires  130 ′,  130 ″ connected to the pulsator  140 . The water in the water tank  110  is filled to the water level  180 . The electrodes  120 ′,  120 ″ are typically immersed below the water level  180 . When voltage is applied across the two electrodes  120 ′,  120 ″ a voltage gradient is impressed across the fish  160 . Depending on the size of the voltage gradient induced in the fish will determine the effect on the fish with the ideal effect being electrotaxis. The effect of electrotaxis on the fish results in the fish being attracted to the cathode, where they are netted. 
         [0048]    The pulsator  140  can be modified to generate a potential difference (e.g. a voltage) which has a steady direct current output, an alternating current output, or a pulsed direct current output. The pulsed direct current output will typically have a duty cycle variation from 0.1% to 50%, but typically a value of 1% is utilized. 
         [0049]    Furthermore, a stream of water can be injected into the tank to orient the fish along an axis that optimizes the potential difference across the body of the fish. 
         [0050]    Now referring to a subportion of the preferred embodiment of the improved holding tank  130 . The improved holding tank  130  has a electroanesthesia (EA) cathode electrode  242  and an electroanesthesia (EA) anode electrode  244  place at opposite ends of the holding tank so that fish  110 ′ are in between the two electrodes. The EA cathode probe  242  and the EA anode probe  244  are connected to the pulsator  140 . The pulsator  140  creates and electrical fields between the EA cathode probe  242  and the EA anode probe  244 . 
         [0051]    To induce electrotaxis a voltage gradient of approximately 1.5 V/cm should be induced across the body of the fish. To anesthetize the fish  160 , (electronarcosis), a voltage gradient of 150-250 V/m (1.5 to 2.5 V/cm) should be induced across the body of the fish  160 . To induce paralysis in the fish (tetany) a greater voltage gradient is needed than electronarcosis. To euthanize the fish  160 , a voltage gradient of 5.0 V/cm or greater should be induced across the body of the fish  160 . The voltage gradients needed for electronarcosis, tetany, and euthanasia vary from fish species to fish species, and of course, differ based on the individual physiology of each fish. Furthermore, the effect on the fish is dependent on the amount of time the fish is exposed to the potential difference. 
         [0052]    Now referring to  FIG. 3  which depicts an implementation of the preferred embodiment using a single pulsator  140 . A single pulsator  140  is configured with a control input  330 , a dpdt (double pole, double throw) switch  310 . The switch  310  is controlled  320  by the pulsator  320  in response to the control input  330 . In one setting the switch  310  it connects the cathode output of the pulsator  140  to the EA cathode electrode  242  and the anode output of the pulsator  140  to the EA anode electrode  244 . In the other setting of the switch  310  it connects the cathode output of the pulsator  140  to the electrofishing cathode  142  and the anode output of the pulsator  140  to the electrofishing anode  144 . 
         [0053]    When the pulsator  140  is connected to the anode and cathode placed in the holding tank  130  and energized, the fish  110 ′ in the holding tank  130  are anesthisized. The process of anesthetization effectively renders the fish motionless without killing the fish. In this state, they may be transported to the inspection table  150 , examined, and returned to the holding tank. The use of an electrical current for anesthesia on the fish  110 ′ is functionally equivalent to the use of chemical anesthesetic agents without the attendant drawbacks of the use of the chemical method. 
         [0054]    Now referring to the flowchart  400  of the operation of the pulsator  140  in response to the control switch as shown in  FIG. 4 . The pulsator checks to see if the electroanesthesia control input  330  is set  440  to direct the pulsator power to the electroanesthesia anode and cathode pair. If the electroanesthesia control input  330  is not set, then the pulsator power will be directed to the electrofishing anode and cathode pair. 
         [0055]    Now referring to  FIG. 5  which shows an alternate embodiment with the holding tank  130 , the pulsator  140 , and a pressure mat  520  surrounding the holding tank  130 . When current is flowing between the cathode  242  and the anode  244 , there exists an opportunity for a person to place their hand  530  or other appendage into the holding tank  130  and thus exposing the hand  530  to the electric field between the cathode  242  and the anode  244 . To prevent this exposure, the pressure mat  510  contains an electrical switch  520  that is closed when a person steps on the map. When the switch  520  is closed an electrical signal is input into the pulsator  140  which deactivates the pulsator. The objective being that the pulsator  140  is disabled when a hand  530  or other appendage is in the vicinity of the holding tank  130 . 
         [0056]    The holding tank  130  can be made of an insulating material, such as plastic, fiberglass, composite resins, and/or other polymers. Also a typical metallic holding tank, such as aluminum can be coated with a plastic to insulate the tank. The tank can also have various attachments, such as, water recirculators and air bubblers to adjust and improve the environment for the aquatic species being anesthetisized. 
         [0057]    Persons skilled in the art will recognize that many modifications and variations are possible in the details, materials, and arrangements of the parts and actions which have been described and illustrated in order to explain the nature of this inventive concept and that such modifications and variations do not depart from the spirit and scope of the teachings and claims contained therein. 
         [0058]    All patent and non-patent literature cited herein is hereby incorporated by references in its entirety for all purposes.