Abstract:
A skincare stimulant having an effective dose of platelets and pharmaceutically acceptable solvents and/or excipients, wherein the effective dose refers to the presence of at least 1000 platelets in every milligram of skincare stimulant.

Description:
FIELD OF THE INVENTION 
       [0001]    The present invention relates to a skincare stimulant and usage thereof, and more particularly to a skincare stimulant using compositions of platelets for ameliorating skin aging and usage thereof. 
       BACKGROUND OF THE INVENTION 
       [0002]    The skincare industry has developed important techniques to resolve problems like wrinkles, loose skins, pock marks, enlarged pores, dark spots, and hyperpigmentation, as can be referred to in the published Taiwan patent 201018492, a skincare composition based on peptides that inhibits the reactiveness of the reactive carbonyl species (RCS) was disclosed, which is applicable to skin, mucous membrane, scalp, and/or hair treatments, and/or skincare; in the published Taiwan patent 200812633, a technique employing whey protein micelles as a grinding agent was disclosed. In the announced Taiwan patents I281405 and I260231, and the published Taiwan patents 200735893 and 200505492, formulations of skincare stimulant using small-molecule chemicals as the active ingredients were disclosed, yet a skincare stimulant using a platelet dry powder as the active ingredient has not been developed so far. In the US patents, the only patent that had disclosed a skincare stimulant using platelets-related substances as the active ingredient is the published patent 20070253940, and the patent disclosed: a skincare stimulant using the platelet-rich plasma as the active ingredient. The present invention is the first to have disclosed: a skincare stimulant utilizing a platelet dry powder as the active ingredient, which achieves actual effects such as anti-wrinkling, removing pigments, reducing skin lines, increasing skin elasticity/tightening skin, and also stimulating and renewing dermal cells to rebuild skin tissues, and allays amyloidosis and keratosis pilaris. In addition, the use of this skincare stimulant achieves stronger, more comprehensive, and longer-lasting effects than that of the traditional methods. 
       SUMMARY OF THE INVENTION 
       [0003]    The invention provides a skincare stimulant using platelets as the active ingredient. 
         [0004]    The invention provides platelets for anti-wrinkling effect. 
         [0005]    The invention provides platelets for removing pigmentation. 
         [0006]    The invention provides platelets for reducing skin lines and increasing skin elasticity/tightening skin. 
         [0007]    The invention provides platelets for stimulating the renewal of dermal cells. 
         [0008]    The invention provides platelets for rebuilding skin tissues. 
         [0009]    The invention provides platelets for ameliorating amyloidosis. 
         [0010]    The invention provides platelets for ameliorating keratosis pilaris. 
         [0011]    The skincare stimulant of the invention includes an effective dose of platelets and pharmaceutically acceptable solvents and/or excipients, wherein the effective dose refers to the presence of at least 1000 platelets in every milligram of skincare stimulant. 
         [0012]    The source of said platelets of the skincare stimulant of the present invention is a platelet dry powder. Said platelet dry powder is prepared from blood or blood preparations like platelet-rich plasma (abbreviated as PRP hereafter) by using specific methods, and comprises intact platelets. The methods for preparing the platelet dry powder may be any of the previously known methods for preparing platelet dry powders, as can be referred to in the announced Taiwan patents I300806 and I270375; the published Taiwan patents 201004659 and 200526680, and the announced U.S. Pat. Nos. 7,659,052, 7,202,020, 7,169,606, 6,060,233, 5,736,313, and 5,589,462. Moreover, the preparation of the platelet dry powder described in this invention is not limited to the methods disclosed in the aforesaid cited references. 
         [0013]    Said platelet dry powder may comprise low amounts of anticoagulants and protectants from using different methods, such as the platelet dry powder prepared according to the method disclosed in the announced Taiwan patent TW-I270375, which includes low amounts of the anticoagulant acid citrate dextrose, cryoprecipitates, and thrombin, but since said components do not actually affect the performance of the platelet dry powder, it is not necessary to eliminate them therefrom. 
         [0014]    Said skincare stimulant may be any of the previously known forms of drugs, such as solutions, suspensions, ointments, powders, and pills; is preferably to be in the form of solutions, ointments, or transdermal patches, and is more preferably to be in the form of sprays or ointments. Moreover, the powders and pills may be prepared into other forms suitable for use in actual applications. 
         [0015]    Said pharmaceutically acceptable solvents refer to any solvents that can be ingested or applied externally by humans or animals, such as alcohol-water co-solvents, water, and saline, and is preferably water or saline. It is also necessary to ensure the amount of solvents added could maintain the effective dose of platelets. 
         [0016]    The pharmaceutically acceptable excipients are the previously known excipients, and applications thereof are determined according to the forms of drugs. 
         [0017]    Said effective dose of the platelets refers to the presence of at least 1000 platelets in every milligram of the skincare stimulant; is preferably to be the presence of at least 3000 platelets therein, and is more preferably to be the presence of at least 5000 platelets therein. Generally, the more the platelets, the more significant the resulted effects. For instance, a skincare stimulant having 20000 platelets in every milligram thereof has been shown to achieve more significant effects than a skincare stimulant having 5000 platelets in every milligram thereof. 
         [0018]    Said skincare stimulant may be added with any effective ingredients that convey positive effects thereto (such as anti-inflammatory ingredients, analgesic ingredients, nutritious ingredients, and/or ingredients promoting absorption), or any ingredients that do not have negative effects or side effects (such as spices). 
         [0019]    Said platelet dry powder may be heterologous, homologous, or autologous platelet dry powders. Considering the users&#39; possible concerns, the platelet dry powder is preferably homologous or autologous platelet dry powders, and is more preferably autologous platelet dry powders. 
         [0020]    Generally speaking, the platelet-derivatives growth factors (PDGF) of platelets in blood are approximately 40-200 μg/mL, as can be referred to in Vogt., et al.,  Determination of endogenous growth factors in human wound healing. Wound Repair Regeneration,  2004, 12(4): p. 485-492. The effectiveness of the skincare stimulant of the invention may be related to the long-term effectiveness of PDGF of the platelet dry powder, but the relationship thereof still requires further investigation. 
         [0021]    A method for stimulating skincare according to the invention, comprising: 
         [0022]    a cleaning step used to make a body area awaiting stimulation of skincare suitable for further treatments; and 
         [0023]    a spraying or spreading step, which sprays or spreads a skincare stimulant having an effective dose of platelets over said cleaned body area, wherein the effective dose refers to the presence of at least 1000 platelets in every milligram of the skincare stimulant. 
         [0024]    Said cleaning step refers to steps like rinsing and disinfecting to induce the body area awaiting the stimulation of skincare into a condition suitable for further treatments. Generally, the need of rinsing body area awaiting treatments depends on the cleanness of the body area, and the body area are preferably rinsed in advance or immediately before treatments. Though disinfecting is not absolutely required, it is preferable to have the body area disinfected thoroughly. 
         [0025]    Said spraying or spreading step refers to spraying or spreading the skincare stimulant having an effective dose of platelet dry powder over said cleaned body area according to the form of the skincare stimulant. For example, if the skincare stimulant is a solution, it can be evenly sprayed on the body area awaiting the stimulation of skincare, and if the skincare stimulant is an ointment, it can be evenly spread on the body area awaiting the stimulation of skincare. 
         [0026]    The details of said skincare stimulant are as described above. 
         [0027]    Said method for stimulating skincare is suitable to be used on full body skin, especially on the exposed skin such as facial and neck skin. 
         [0028]    To enhance the effects of skincare stimulation, a further absorption-enhancing step may be carried out once or multiple times, and said step may be added before, during, or after the spraying or spreading step. Said absorption-enhancing step may be applied in any ways that facilitates the absorption of a skincare stimulant, such as the methods of iontophoresis, laser treament, electromagnetic wave guiding, micro-needle guiding, physical stimulation, and tap massaging that are previously known in the skincare industry. Before the spraying or spreading step, said absorption-enhancing step is preferably carried out by using laser treatment, micro-needle guiding, and physical stimulation (so as to create micro wounds in the stratum corneum to facilitate absorption). The spraying or spreading step may also be carried out by using iontophoresis or electromagnetic wave guiding to facilitate absorption. After the spraying or spreading step, the absorption-enhancing step is preferably carried out by using electromagnetic wave guiding or tap massaging (so as to induce blood circulation to local skin and facilitate absorption). 
     
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         [0029]      FIGS. 1   a  and  1   b  are photographs showing the patient in Embodiment 1 before and after the treatments. 
           [0030]      FIGS. 2   a  and  2   b  are photographs showing the patient in Embodiment 2 before and after the treatments. 
           [0031]      FIGS. 3   a  and  3   b  are photographs showing the patient in Embodiment 3 before and after the treatments. 
           [0032]      FIGS. 4   a  and  4   b  are photographs showing the patient in Embodiment 4 before and after the treatments. 
           [0033]      FIGS. 5   a  and  5   b  are photographs showing the patient in Embodiment 5 before and after the treatments. 
           [0034]      FIGS. 6   a  and  6   b  are photographs showing the patient in Embodiment 6 before and after the treatments. 
           [0035]      FIGS. 7   a  and  7   b  are photographs showing the patient in Embodiment 7 before and after the treatments. 
           [0036]      FIGS. 8   a  and  8   b  are photographs showing the patient in Embodiment 8 before and after the treatments. 
           [0037]      FIGS. 9   a  and  9   b  are photographs showing the patient in Embodiment 9 before and after the treatments. 
           [0038]      FIGS. 10   a  and  10   b  are photographs showing the patient in Embodiment 10 before and after the treatments. 
           [0039]      FIGS. 11   a  and  11   b  are photographs showing the patient in Embodiment 11 before and after the treatments. 
           [0040]      FIGS. 12   a  and  12   b  are photographs showing the patient in Embodiment 12 before and after the treatments. 
       
    
    
     DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS 
       [0041]    The preferred embodiments are described hereafter in order to further elucidate the techniques of the invention: 
       TESTING EXAMPLE  
       [0042]    The method disclosed in the announced Taiwan patent TW-I270375 was used to prepare a platelet dry powder. 1.0 g of platelet dry powder was obtained and added to saline to make up 5.0 mL of solution, then the PDGF titer of the solution was analyzed by using a spectrophotometer (U.S. Bio-Tek Instruments, Inc., Model μ-Quant) immediately after preparation (0 hour), after 1 week (168 hours), after 2 weeks (336 hours), after 3 weeks (504 hours), and after 4 weeks (672 hours). Results from the analyses are shown in Table 1. 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Changes of PDGF titer in the solution prepared from the platelet dry 
               
               
                 powder. 
               
             
          
           
               
                   
                 Weeks 
               
             
          
           
               
                   
                 0 
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
               
               
                   
                   
               
             
          
           
               
                 PDGF 
                 2930 
                 2795 
                 3012 
                 2854 
                 2822 
                 2776 
                 2785 
               
               
                 (pg/mL) 
               
               
                   
               
             
          
         
       
     
         [0043]    The experimental figures show: The PDGF titer of the platelet dry powder is very stable. 
       Embodiment 1  
       [0044]    The method disclosed in the announced patent TW-I270375 was used to prepare an autologous platelet dry powder from autologous blood in advance. The platelet dry powder was added to water obtained by reverse osmosis (RO) to prepare a solution having 5000 platelets/μL therein, then the solution was held in a spray bottle. The number of platelets in the solution was measured by using a Hematology Analyzer (Manufacturer: Sysmex, Model: KX-21). 
         [0045]    A patient was allowed to undergo a treatment for skincare stimulation once a week, which involved firstly disinfecting a body area awaiting the skincare stimulation, and then carrying out laser treatment on the disinfected body area, followed by evenly spraying the skincare stimulant (solution) on the disinfected body area. In addition, between every two treatments, the body area awaiting the skincare stimulation was evenly sprayed with the skincare stimulant after washing face everyday. 
         [0046]    The  FIGS. 1   a  and  1   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 4 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation showed significantly increased skin elasticity and firmness; the pores became smaller and the skin became whiter and clearer. 
       Embodiment 2 
       [0047]    Similar to Embodiment 1, the  FIGS. 2   a  and  2   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 7 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results indicated: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, showed significantly increased skin elasticity and firmness; the pores became smaller and the skin became whiter and clearer. 
       Embodiment 3  
       [0048]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 10000. The  FIGS. 3   a  and  3   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 2 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness, and the pores became smaller while the skin became whiter and clearer. 
       Embodiment 4  
       [0049]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 5000. The  FIGS. 4   a  and  4   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 4 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness, and the pores became smaller and the skin became whiter and clearer. 
       Embodiment 5  
       [0050]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 5000. The  FIGS. 5   a  and  5   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 4 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent; scars thereon became lighter (please refer to the picture between  FIGS. 5   a  and  5   b ); showed significantly increased skin elasticity and firmness; the pores became smaller and the skin became whiter and clearer. 
       Embodiment 6  
       [0051]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 5000. The  FIGS. 6   a  and  6   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 7 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness, and the pores became smaller and the skin became whiter and clearer. 
       Embodiment 7  
       [0052]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 5000. The  FIGS. 7   a  and  7   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 7 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness, and the pores became smaller and the skin became whiter and clearer. 
       Embodiment 8  
       [0053]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 3000. The  FIGS. 8   a  and  8   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 24 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness, and the pores became smaller while the skin became whiter and clearer. 
       Embodiment 9 
       [0054]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 3000. The  FIGS. 9   a  and  9   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 21 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness, and the pores became smaller while the skin became whiter and clearer. 
       Embodiment 10  
       [0055]    Similar to Embodiment 1, but the platelet dry powder was a heterologous platelet dry powder prepared from bovine blood, and the concentration of the skincare stimulant (platelet number/μL of solution) was 5000. The  FIGS. 10   a  and  10   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 3 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness, and the pores became smaller and the skin became whiter and clearer. 
       Embodiment 11 
       [0056]    Similar to Embodiment 1, but the platelet dry powder was a heterologous platelet dry powder prepared from bovine blood, and the concentration of the skincare stimulant (platelet number/μL of solution) was 3000. The  FIGS. 11   a  and  11   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 8 weeks after the treatments, wherein the photographs on the right are the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness; the pores became smaller and the skin became whiter and clearer. 
       Embodiment 12  
       [0057]    Similar to Embodiment 1, but the platelet dry powder was a homologous platelet dry powder prepared from the blood in a blood bank, and the concentration of the skincare stimulant (platelet number/μL of solution) was 1000. The  FIGS. 12   a  and  12   b  show the conditions of the body area awaiting the skincare stimulation before the treatments and 19 weeks after the treatments, wherein the photographs on the right re the partially magnified pictures of the photographs on the left. The results showed: the body area awaiting the skincare stimulation had wrinkles that either vanished or became less apparent, and spots thereon became lighter in color; showed significantly increased skin elasticity and firmness; the pores became smaller, and the skin became whiter and clearer. 
         [0058]    The results from the embodiments indicate that: with respect to the effects of the treatments, the heterologous PLT appeared to outperform the homologous PLT and the autologous PLT. However, considering the patients&#39; possible concerns, it is preferable to use homologous PLT or autologous PLT, and is more preferable to use autologous PLT.