Abstract:
The disclosure relates to certain novel substituted ring-fused thiazolino 2-pyridines of Formula I, to processes for preparing such compounds, to their use in treating a bacterial infection such as  Chlamydia  infection, to methods for their therapeutic use and to pharmaceutical compositions containing them.

Description:
TECHNICAL FIELD 
       [0001]    The present disclosure relates to certain novel substituted ring-fused thiazolino 2-pyridones, to processes for preparing such compounds, to their use in treating  Chlamydia  infections, to methods for their therapeutic use and to a pharmaceutical composition containing any such compound. 
       BACKGROUND 
       [0002]      Chlamydia  infection is a common sexually transmitted infection in humans caused by the bacterium  Chlamydia trachomatis . Both men and women may be infected, and symptoms include pain when urinating, heavier periods than normal in women and pain in the testicles in men. Commonly, however, no symptoms are noticeable or symptoms appear only after the infection has spread significantly. If left untreated, there is a considerable risk that the infected person not only passes the infection on to other people but also will suffer from severe complications. 
         [0003]    In women, an untreated chlamydia infection can spread to the cervix, uterus and womb and cause pelvic inflammatory disease (PID). PID is a major cause of infertility, miscarriage and ectopic pregnancy. Further, the infant of a chlamydia infected pregnant woman may develop chlamydia-related conjunctivitis and pneumonia. 
         [0004]    In men, an untreated chlamydia infection may lead to urethritis (i.e. inflammation of the urethra), orchitis (i.e. swollen testicles), reactive arthritis, and infertility. 
         [0005]    Due to the serious consequences of chlamydia infection health care authorities in many countries recommend and arrange for screening of parts of the population considered at risk for infection. When detected,  Chlamydia trachomatis  infection can mostly be effectively cured with antibiotics such as azithromycin, doxycycline, erythromycin, amoxicillin or ofloxacin. 
         [0006]    However, there is increasing concern about antibiotic resistance, i.e. the fact that some bacteria cannot be controlled or killed by antibiotics, which is considered a major threat to public health. 
         [0007]    PCT/SE2014/050584 discloses substituted ring-fused thiazolino 2-pyridones useful in the treatment of  Chlamydia  infection. 
         [0008]    “A 2-Pyridone-Amide Inhibitor Targets the Glucose Metabolism Pathway of  Chlamydia trachomatis ” (mBio vol. 6 no. 1, e02304-14 (doi:10.1128/mBio.02304-14) Patrik Engström et al 2015, discloses substituted ring-fused thiazolino 2-pyridones useful in the treatment of  Chlamydia  infection. The compound named 7-(naphthalen-1-ylmethyl)-8-cyclopropyl-5-oxo-N-phenyl-5H-[1,3]thiazolo[3,2-a]pyridine-3-carboxamide (KSK 120) is identified as a lead compound, and is said to target glucose metabolism of  Chlamydia trachomatis  by targeting the inner membrane localized hexose-phosphate transporter. 
         [0009]    There is a need for alternative treatments of bacterial infections such as  Chlamydia  infection that may be used alone or in conjunction with any existing treatment. 
         [0010]    It is an object of the present disclosure to provide compounds useful in the treatment, prevention and/or alleviation of bacterial infections such as  Chlamydia  infection. 
       SUMMARY 
       [0011]    In accordance with the present disclosure there is provided a compound of Formula I: The present disclosure provides compounds of Formula I 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or a pharmaceutically acceptable salt thereof,
 
wherein
       R 1  is selected from the group consisting of   a) C(O)NR 5 R 6 ,   b) C(O)OH,   c) C(O)SO 2 R 8 ,   and   d)       
 
         [0000]    
       
                 
         
             
             
         
       
       
         
           
             R 2  is selected from the group consisting of 
             a) OH, 
             b) NZ 1 Z 2 , 
             c) C 1 -C 4 alkoxy substituted with 0, 1, 2 or 3 substituents independently selected from the group consisting of F and Cl, 
             d) cyclopropoxy, cyclopropylmethoxy, phenyloxy, 2-pyridinyloxy or 4-pyridinyloxy each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methyl, ethyl, n-propyl, isopropyl, trifluoromethyl, methoxy, CH 3 SO 2 O and phenylSO 2 O, 
             e) C 1 -C 3 alkylthio, C 1 -C 3 alkylS(O) and C 1 -C 3 alkylS(O) 2 , phenylthio, phenylS(O) or phenylS(O) 2  each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, 
             f) 2-pyridinylS(O) 2  substituted with 0, 1 or 2 substituents in positions 3, 4 or 5 independently selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, 
             g) 4-pyridinylS(O) 2  substituted with 0, 1 or 2 substituents in positions 2, 3 or 5 independently selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, 
             h) Cl or F, 
             and 
             i) CF 3 ; 
             R 3  is selected from the group consisting of 
             a) CX 1 X 2 phenyl, CH 2 Ophenyl, CX 1 X 2 -(2)-pyridyl, CX 1 X 2 -(3)-pyridyl or CX 1 X 2 -(4)-pyridyl, each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, trifluoromethyl and methyl, 
             b) 1,3-dioxa-5-indanyl-methylene, 
             c) 1-naftyl-methylene or 1-naftyl-4-methyl-methylene, and 
             d) 7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl-methylene; 
             R 4  is selected from the group consisting of: 
             a) hydrogen, and 
             b) NY 1 Y 2 . 
           
         
       
     
         [0037]    The following applies in the above definitions.
       R 5  represents hydrogen or C 1 -C 6 alkyl, or   R 5  represents phenyl, 2-pyridinyl or 4-pyridinyl each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl.   R 6  represents hydrogen, C 1 -C 4 alkyl or C 1 -C 6 cycloalkyl.   R 7  represents phenyl or 2-pyridinyl each independently substituted with 0, 1 or 2 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl.   R 8  represents C 1 -C 3 alkyl, phenyl, 2-pyridinyl or 4-pyridinyl each independently substituted with 0, 1 or 2 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl.   X 1  and X 2  each independently represents hydrogen, OH, halogen, oxo or NH 2 .   Y 1  and Y 2  each independently represents hydrogen, methyl, CH 3 S(O) 2  or C(O)CH 3 , or Y 1  and Y 2  together form CH 2 CH 2 CH 2 CH 2  or CH 2 CH 2 CH 2 CH 2 CH 2 .   Z 1  and Z 2  each independently represents hydrogen, C 1 -C 4 alkyl, C 3 -C 5 cycloalkyl, C(O)CH 3 , C(O)OCH 3 , CH 3 S(O) 2  or phenylS(O) 2 , or Z 1  and Z 2  together form CH 2 CH 2 CH 2 , CH 2 CH 2 CH 2 CH 2  or CH 2 CH 2 CH 2 CH 2 CH 2 .       
 
         [0046]    There is also provided a compound as described herein such as a compound of Formula I, any of its stereoisomers, any mixture of its stereoisomers, or a pharmaceutically acceptable salt thereof, for use as a medicament in therapy. 
         [0047]    There is also provided a pharmaceutical composition comprising a therapeutically effective amount of a compound as described herein such as Formula I as defined herein, any of its stereoisomers, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable adjuvant, diluent or carrier. 
         [0048]    There is also provided a compound as described herein such as a compound of Formula I, any of its stereoisomers, any mixture of its stereoisomers, or a pharmaceutically acceptable salt thereof, for use in the treatment, prevention and/or alleviation of a bacterial infection. 
         [0049]    There is also provided a use of a compound as described herein such as a compound of Formula I, any of its stereoisomers, any mixture of its stereoisomers, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for use in the treatment, prevention and/or alleviation of a bacterial infection. 
         [0050]    There is also provided a method of treatment, prevention and/or alleviation of a bacterial infection comprising administering to a mammal, including a human, in need thereof an effective amount of a compound as described herein such as a compound of Formula I, any of its stereoisomers, any mixture of its stereoisomers, or pharmaceutically acceptable salt thereof. 
     
    
     DESCRIPTION 
       [0051]    The present disclosure provides compounds of Formula I: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or a pharmaceutically acceptable salt thereof,
 
wherein
       R 1  is selected from the group consisting of   a) C(O)NR 5 R 6 ,   b) C(O)OH,   c) C(O)SO 2 R 8 ,   and   d)       
 
         [0000]    
       
                 
         
             
             
         
       
       
         
           
             R 2  is selected from the group consisting of 
             a) OH, 
             b) NZ 1 Z 2 , 
             c) C 1 -C 4 alkoxy substituted with 0, 1, 2 or 3 substituents independently selected from the group consisting of F and Cl, 
             d) cyclopropoxy, cyclopropylmethoxy, phenyloxy, 2-pyridinyloxy or 4-pyridinyloxy each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methyl, ethyl, n-propyl, isopropyl, trifluoromethyl, methoxy, CH 3 SO 2 O and phenylSO 2 O, 
             e) C 1 -C 3 alkylthio, C 1 -C 3 alkylS(O) and C 1 -C 3 alkylS(O) 2 , phenylthio, phenylS(O) or phenylS(O) 2  each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, 
             f) 2-pyridinylS(O) 2  substituted with 0, 1 or 2 substituents in positions 3, 4 or 5 independently selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, 
             g) 4-pyridinylS(O) 2  substituted with 0, 1 or 2 substituents in positions 2, 3 or 5 independently selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, 
             h) Cl or F, 
             and 
             i) CF 3 , 
             R 3  is selected from the group consisting of 
             a) CX 1 X 2 phenyl, CH 2 Ophenyl, CX 1 X 2 -(2)-pyridyl, CX 1 X 2 -(3)-pyridyl or CX 1 X 2 -(4)-pyridyl, each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, trifluoromethyl and methyl, 
             b) 1,3-dioxa-5-indanyl-methylene, 
             c) 1-naftyl-methylene or 1-naftyl-4-methyl-methylene, 
             and 
             d) 7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl-methylene; 
             R 4  is selected from the group consisting of: 
             a) hydrogen, and 
             b) NY 1 Y 2 . 
           
         
       
     
         [0078]    The following definitions shall apply throughout the description and the appended claims unless specifically stated otherwise.
   R 5  represents hydrogen or C 1 -C 6 alkyl,
 
or
   R 5  represents phenyl, 2-pyridinyl or 4-pyridinyl each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl.   R 6  represents hydrogen, C 1 -C 4 alkyl or C 1 -C 6 cycloalkyl.   R 7  represents phenyl or 2-pyridinyl each independently substituted with 0, 1 or 2 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl.   R 8  represents C 1 -C 3 alkyl, phenyl, 2-pyridinyl or 4-pyridinyl each independently substituted with 0, 1 or 2 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl.   X 1  and X 2  each independently represents hydrogen, OH, halogen, oxo or NH 2 .   Y 1  and Y 2  each independently represents hydrogen, methyl, CH 3 S(O) 2  or C(O)CH 3 , or Y, and Y 2  together form CH 2 CH 2 CH 2 CH 2  or CH 2 CH 2 CH 2 CH 2 CH 2 .   Z 1  and Z 2  each independently represents hydrogen, C 1 -C 4 alkyl, C 3 -C 5 cycloalkyl, C(O)CH 3 , C(O)OCH 3 , CH 3 S(O) 2  or phenylS(O) 2 , or Z 1  and Z 2  together form CH 2 CH 2 CH 2 , CH 2 CH 2 CH 2 CH 2  or CH 2 CH 2 CH 2 CH 2 CH 2 .   
 
         [0087]    The term “C 1 -C 3 alkyl” denotes a straight or branched, saturated or unsaturated, alkyl group of one to three carbon atoms. Examples of “C 1 -C 3 alkyl” include, but are not limited to, methyl, ethyl, vinyl, allyl, n-propyl and isopropyl. 
         [0088]    The term “C 1 -C 4 alkyl” denotes a straight or branched, saturated or unsaturated, alkyl group of one to four carbon atoms. Examples of “C 1 -C 4 alkyl” include, but are not limited to, methyl, ethyl, vinyl, allyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl and tert-butyl. 
         [0089]    The term “C 1 -C 6 alkyl” denotes a straight or branched, saturated or unsaturated, alkyl group of one to six carbon atoms. Examples of “C 1 -C 6 alkyl” include, but are not limited to, methyl, ethyl, vinyl, allyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, 1,1-dimethylpropyl, 2,2-dimethylpropyl, 3-methylbutyl, 1-methylbutyl and 1-ethylpropyl, n-hexyl, 2-methylpentyl, 3-methylpentyl, 2,3-dimethylbutyl and 2,2-dimethylbutyl. 
         [0090]    The term “C 3 -C 5 cycloalkyl” denotes a saturated or unsaturated non-aromatic monocyclic ring composed of three, four or five carbons. Examples of “C 3 -C 5 cycloalkyl” include, but are not limited to, cyclopropyl, cyclobutyl and cyclopentyl. 
         [0091]    The term “C 1 -C 6  cycloalkyl” denotes a saturated or unsaturated non-aromatic monocyclic ring composed of three, four, five or six carbons. Examples of “C 3 -C 5 cycloalkyl” include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl. 
         [0092]    The term “halogen” denotes fluoro, chloro, bromo and iodo. 
         [0093]    The term “C 1 -C 3 alkoxy” denotes a C 1 -C 3 alkyl group as described herein which is linked to an oxygen. Examples of “C 1 -C 3 alkoxy” include, but are not limited to, methoxy, ethoxy, n-propoxy and iso-propoxy. 
         [0094]    The term “C 1 -C 4 alkoxy” denotes a C 1 -C 4 alkyl group as described herein which is linked to an oxygen. Examples of “C 1 -C 4 alkoxy” include, but are not limited to, methoxy, ethoxy, n-propoxy, iso-propoxy, and butoxy. 
         [0095]    Further, the present disclosure provides compounds of Formula I 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or a pharmaceutically acceptable salt thereof
 
wherein
       R 1  is selected from the group consisting of   a) C(O)NR 5 R 6 ,   b) C(O)OH,   c) C(O)SO 2 R 8 ,   and   d)       
 
         [0000]    
       
                 
         
             
             
         
       
       
         
           
             R 2  is selected from the group consisting of 
             a) OH, 
             b) NZ 1 Z 2 , 
             c) C 1 -C 3 alkoxy substituted with 0, 1, 2 or 3 substituents independently selected from the group consisting of F and Cl, 
             d) cyclopropoxy, phenyloxy, 2-pyridinyloxy or 4-pyridinyloxy each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methyl, ethyl, n-propyl, isopropyl, trifluoromethyl, methoxy, CH 3 SO 2 O and phenylSO 2 O, 
             e) C 1 -C 3 alkylthio, C 1 -C 3 alkylS(O) and C 1 -C 3 alkylS(O) 2 , phenylthio, phenylS(O) or phenylS(O) 2  each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, 
             f) 2-pyridinylS(O) 2  substituted with 0, 1 or 2 substituents in positions 3, 4 or 5 independently selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl, and 
             g) 4-pyridinylS(O) 2  substituted with 0, 1 or 2 substituents in positions 2, 3 or 5 independently selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl; 
             R 3  is selected from the group consisting of 
             a) CX 1 X 2 phenyl, CH 2 Ophenyl, CX 1 X 2 -(2)-pyridyl, CX 1 X 2 -(3)-pyridyl or CX 1 X 2 -(4)-pyridyl, each independently substituted with 0, 1, 2 or 3 substituents selected from the group consisting of F, Cl, methoxy, trifluoromethyl and methyl, 
             b) 1,3-dioxa-5-indanyl-methylene, 
             c) 1-naftyl-methylene, 
             and 
             d) 7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl-methylene; 
             R 4  is selected from the group consisting of: 
             a) hydrogen, 
             and 
             b) NY 1 Y 2 . 
           
         
       
     
         [0120]    The compound of Formula I may exist as stereoisomers such as a compound of Formula Ia or a compound of Formula Ib. 
         [0000]    
       
                 
         
             
             
         
       
     
         [0121]    It will be appreciated that the following compounds are excluded from the scope of the compound of Formula I or Formula Ia: (3R)-7-methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid, (3R)-6-[(1,3-dioxa-5-indanyl)methyl]-7-methoxy-4-oxo-1-thia-3a-aza-3-indancarboxylic acid and (3R)-7-methoxy-4-oxo-6-{(7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl)methyl}-1-thia-3a-aza-3-indancarboxylic acid. 
         [0122]    Specific values of R 1 , R 2 , R 3  and R 4  of the compound of Formula I, any of its stereoisomers or any mixture of its stereoisomers, or a pharmaceutically acceptable salt thereof, will now follow. It will be understood that these values may be used with any of the definitions, claims or embodiments described herein. 
         [0123]    The present disclosure relates to compounds of Formula I, wherein: 
         [0124]    R 1  may be C(O)NR 5 R 6 . R 5  may be hydrogen, and R 6  may be phenyl or 5-chloro-2-pyridyl. 
         [0125]    R 1  may be C(O)OH. 
         [0126]    Further, R 1  may be C(O)SO 2 R 8 . 
         [0127]    Further, R 1  may be 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    in which R 7  is phenyl or 2-pyridinyl each independently substituted with 0, 1 or 2 substituents independently selected from the group consisting of F, Cl, methoxy, methyl and trifluoromethyl. In an embodiment, R 7  may be phenyl. 
         [0128]    For instance, R 1  may be selected from the group consisting of 3-phenyl-1,2,4-oxadiazol-5-y,4-Phenyl-1H-1,2,3-triazol-1-yl, 5-phenyl-1,2,4-oxadiazol-3-yl, 5-phenyl-1,3,4-oxadiazol-2-yl,anilinoformaldehyde and 5-chloro-2-pyridylamino-formaldehyde. 
         [0129]    R 2  may be C 1 -C 3 alkoxy independently substituted with 0, 1, 2 or 3 substituents from the group consisting of F or Cl. For instance, R 2  may be methoxy, ethoxy or trifluoromethoxy. 
         [0130]    Further, R 2  may be NZ 1 Z 2 . For instance, R 2  may be acetylamino, dimethylamino, methylsulfonyl, methylsulfonylamino and phenylsulfonylamino. 
         [0131]    Further, R 2  may be selected from the group consisting of methoxy, ethoxy, cyclopropoxy, trifluoromethoxy, 2,2,2-trifluoroethoxy, phenoxy methylsulfonyloxy, phenylsulfonyloxy, acetylamino, dimethylamino, methylsulfonyl, methylsulfonylamino and phenylsulfonylamino. 
         [0132]    R 3  may be selected from the group consisting of 1-naphthyl-methylene, 1-naphthyloxymethylene, 1,3-dioxa-5-indanyl-methylene, 2,3-xylylmethylene, 2,3-xylyloxy-methylene, 7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl-methyl and o-tolyl-methylene. 
         [0133]    R 4  may be selected from the group consisting of hydrogen, amino, acetylamino and methylsulfonylamino. 
         [0134]    There is also provided a compound of Formula I such as a compound of Formula Ia, or a pharmaceutically acceptable salt thereof, wherein
   R 1  is selected from the selected from the group consisting of 3-phenyl-1,2,4-oxadiazol-5-y,4-phenyl-1H-1,2,3-triazol-1-yl, 5-phenyl-1,2,4-oxadiazol-3-yl, 5-phenyl-1,3,4-oxadiazol-2-yl,anilinoformaldehyde and 5-chloro-2-pyridylamino-formaldehyde,   R 2  is selected from the group consisting of methoxy, cyclopropylmethoxy, trifluoromethoxy, 2,2,2-trifluoroethoxy, phenoxy methylsulfonyloxy, phenylsulfonyloxy, acetylamino, dimethylamino, methylsulfonyl, methylsulfonylamino and phenylsulfonylamino   R 3  is selected from the group consisting of 1-naphthyl-methylene, 1-naphthyloxymethylene, 1,3-dioxa-5-indanyl-methylene, 2,3-xylylmethylene, 2,3-xylyloxy-methylene, 7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl-methyl and o-tolyl-methylene, and   R 4  is selected from the group consisting of hydrogen, amino, acetylamino and methylsulfonylamino.   
 
         [0139]    There is also provided a compound of Formula I such as a compound of Formula Ia, or a pharmaceutically acceptable salt thereof, wherein
   R 1  is selected from the group consisting of (3-fluoro-5-toluidino)formaldehyde, (p-toluidino)formaldehyde, anilinoformaldehyde, 3-phenyl-1,2,4-oxadiazol-5-yl, (5-chloro-2-pyridylamino)formaldehyde, 5-phenyl-1,3,4-oxadiazol-2-yl, 5-phenyl-2H-1,2,4-triazol-3-yl and carboxylic acid;   R 2  is selected from the group consisting of methoxy, chloro, isopropylamino, dimethylamino, methanesulfonylamino, methoxycarbonylamino, hydroxy, acetylamino, isobutoxy, cyclopropylmethoxy, ethoxy, allyloxy, m-(trifluoromethyl)phenylsulfonylamino, trifluoromethyl, benzoylamino, methysulfonyl, phenylsulfonylamino and amino   R 3  is selected from the group consisting of 2,3-xylyl)methyl or (1-naphthyl)methyl, p-chlorophenyl)methyl, (2,3-xylyloxy)methyl or (4-methyl-1-naphthyl)methyl; and   R 4  is selected from the group consisting of hydrogen and amino.   
 
         [0144]    There is also provided a compound of Formula I such as a compound of Formula Ia, or a pharmaceutically acceptable salt thereof, wherein
   R 1  is selected from the group consisting of (3-fluoro-5-toluidino)formaldehyde, (p-toluidino)formaldehyde, anilinoformaldehyde, 3-phenyl-1,2,4-oxadiazol-5-yl, (5-chloro-2-pyridylamino)formaldehyde, and 5-phenyl-1,3,4-oxadiazol-2-yl,   R 2  is selected from the group consisting of methoxy, chloro, isopropylamino, dimethylamino, methanesulfonylamino, methoxycarbonylamino, hydroxy, acetylamino, isobutoxy, cyclopropylmethoxy, ethoxy, and allyloxy;   R 3  is selected from the group consisting of (2,3-xylyl)methyl or (1-naphthyl)methyl, p-chlorophenyl)methyl, (2,3-xylyloxy)methyl and (4-methyl-1-naphthyl)methyl; and   R 4  is selected from hydrogen or amino.   
 
         [0149]    There is also provided a compound of Formula I such as a compound of Formula Ia, or a pharmaceutically acceptable salt thereof, wherein
   R 1  is selected from the group consisting of (3-fluoro-5-toluidino)formaldehyde and (p-toluidino)formaldehyde,   R 2  is selected from the group consisting of methoxy, chloro, isopropylamino, dimethylamino, methanesulfonylamino, and methoxycarbonylamino;   R 3  is selected from the group consisting of (2,3-xylyl)methyl, (1-naphthyl)methyl, and p-chlorophenyl)methyl; and   R 4  is selected from the group consisting of hydrogen and amino.   
 
         [0154]    Examples of compounds of Formula Ia include the following compounds, or a pharmaceutically acceptable salt thereof. The compounds below may exist as the corresponding 3S stereoisomer or as a mixture of the 3R and 3S stereoisomers.
   (3R)-7-Methoxy-3-(5-phenyl-1,3,4-oxadiazol-2-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone   (3R)-3-[4-Phenyl-1H-1,2,3-triazol-1-yl]-5-amino-7-methoxy-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone   (3R)-3-[4-Phenyl-1H-1,2,3-triazol-1-yl]-6-[(1,3-dioxa-5-indanyl)methyl]-7-methoxy-1-thia-3a-aza-4-indanone   (3R)-3-[4-Phenyl-1H-1,2,3-triazol-1-yl]-7-methoxy-6-{(7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl)methyl}-1-thia-3a-aza-4-indanone   (3R)-5-Amino-7-methoxy-3-(5-phenyl-1,2,4-oxadiazol-3-yl)-6-[(o-tolyl)methyl]-1-thia-3a-aza-4-indanone   (3R)-7-Methoxy-3-(5-phenyl-1,2,4-oxadiazol-3-yl)-6-[(o-tolyl)methyl]-1-thia-3a-aza-4-indanone   (3R)-3-(5-Phenyl-1,2,4-oxadiazol-3-yl)-6-[(o-tolyl)methyl]-7-trifluoromethoxy-1-thia-3a-aza-4-indanone   (3R)-5-Amino-7-methoxy-3-(3-phenyl-1,2,4-oxadiazol-5-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone   (3R)-3-[4-Phenyl-1H-1,2,3-triazol-1-yl]-5-amino-7-methoxy-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-4-indanone   (3R)-3-[4-Phenyl-1H-1,2,3-triazol-1-yl]-5-amino-7-methoxy-6-[(1-naphthyloxy)methyl]-1-thia-3a-aza-4-indanone   (3R)-3-[4-Phenyl-1H-1,2,3-triazol-1-yl]-7-methoxy-6-[(1-naphthyloxy)methyl]-1-thia-3a-aza-4-indanone   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde   {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   [(3R)-5-Amino-7-cyclopropoxy-4-oxo-6-{(7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl)methyl}-1-thia-3a-aza-3-indanyl]anilinoformaldehyde   [(3R)-7-Methoxy-4-oxo-6-{(7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl)methyl}-1-thia-3a-aza-3-indanyl]anilinoformaldehyde   {(3R)-6-[(1,3-Dioxa-5-indanyl)methyl]-7-methoxy-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   {(3R)-6-[(1,3-Dioxa-5-indanyl)methyl]-7-methoxy-4-oxo-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde   [(3R)-7-Methoxy-4-oxo-6-{(7-thiabicyclo[4.3.0]nona-1,3,5,8-tetraen-9-yl)methyl}-1-thia-3a-aza-3-indanyl](5-chloro-2-pyridylamino)formaldehyde   {(3R)-6-[(1,3-Dioxa-5-indanyl)methyl]-7-methoxy-4-oxo-1-thia-3a-aza-3-indanyl}(5-50 chloro-2-pyridylamino)formaldehyde   {(3R)-7-(Dimethylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde   {(3R)-5-Amino-7-(methylsulfonyl)-6-[(1-naphthyloxy)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   {(3R)-6-[(1,3-Dioxa-5-indanyl)methyl]-7-(methylsulfonyloxy)-4-oxo-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde   {(3R)-6-[(1,3-Dioxa-5-indanyl)methyl]-4-oxo-7-(phenylsulfonyloxy)-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   {(3R)-5-Amino-6-[(1,3-dioxa-5-indanyl)methyl]-4-oxo-7-(phenylsulfonylamino)-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   1-{(3R)-3-Anilinocarbonyl-6-[(1,3-dioxa-5-indanyl)methyl]-7-(methylsulfonylamino)-4-oxo-1-thia-3a-aza-5-indanylamino}-1-ethanone   1-{(3R)-3-Anilinocarbonyl-6-[(1,3-dioxa-5-indanyl)methyl]-5-(methylsulfonylamino)-4-oxo-1-thia-3a-aza-7-indanylamino}-1-ethanone   {(3R)-5-Amino-6-[(1,3-dioxa-5-indanyl)methyl]-4-oxo-7-phenoxy-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   {(3R)-7-Cyclopropyl-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   {(3R)-4-Oxo-7-trifluoromethoxy-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}(4-methyl-2-pyridylamino)formaldehyde   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}(3-fluorotoluidino)formaldehyde and   (3R)-7-(Methylsulfonyloxy)-3-(5-phenyl-2H-1,2,4-triazol-3-yl)-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-4-indanone.   
 
         [0186]    Further, the following compounds are examples of compounds of Formula I or a pharmaceutically acceptable salt thereof. The compounds below may also exist as the corresponding 3S stereoisomer or as a mixture of the 3R and 3S stereoisomers.
   {(3R)-7-Cyclopropyl-4-oxo-6-[(5-quinolyl)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde   {(3R)-7-Cyclopropyl-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde   {(3R)-6-[(p-Chlorophenyl)methyl]-7-cyclopropyl-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde and   (3R)-7-Cyclopropyl-6-[(1-naphthyl)methyl]-3-(5-phenyl-2H-1,2,4-triazol-3-yl)-1-thia-3a-aza-4-indanone.   
 
         [0191]    Further, the following compounds are examples of compounds of Formula I or a pharmaceutically acceptable salt thereof. The compounds below may also exist as the corresponding 3S stereoisomer or as a mixture of the 3R and 3S stereoisomers.
   {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(3-fluoro-5-toluidino)formaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-5-Amino-7-methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(3-fluoro-5-toluidino)formaldehyde;   {(3R)-5-Amino-7-methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-7-Hydroxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-7-Chloro-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-6-[(1-Naphthyl)methyl]-4-oxo-7-(trifluoromethyl)-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   (3R)-5-Amino-7-methoxy-3-(3-phenyl-1,2,4-oxadiazol-5-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone;   (3R)-7-Methoxy-3-(5-phenyl-1,3,4-oxadiazol-2-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone;   (3R)-7-Methoxy-3-(3-phenyl-1,2,4-oxadiazol-5-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde;   Anilino{7-(isopropylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}formaldehyde;   {(3R)-7-(Dimethylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   Anilino{7-(methylsulfonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}formaldehyde;   1-{3-Anilinocarbonyl-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-7-indanylamino}-1-ethanone;   {(3R)-7-(Methoxycarbonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   (3R)-7-(Cyclopropylmethoxy)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-6-[(1-Naphthyl)methyl]-4-oxo-7-(trifluoromethyl)-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-7-Isobutoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-7-(Dimethylamino)-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-7-(Dimethylamino)-6-[(4-methyl-1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid; and   (3R)-7-(Isopropylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid.   
 
         [0221]    Further, the following compounds are examples of compounds of Formula I or a pharmaceutically acceptable salt thereof. The compounds below may also exist as the corresponding 3S stereoisomer or as a mixture of the 3R and 3S stereoisomers.
   {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(3-fluoro-5-toluidino)formaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-7-Chloro-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde; and   {(3R)-6-[(1-Naphthyl)methyl]-4-oxo-7-(trifluoromethyl)-1-thia-3a-aza-3-indanyl}anilinoformaldehyde.   
 
         [0229]    Further, the following compounds are examples of compounds of Formula I or a pharmaceutically acceptable salt thereof. The compounds below may also exist as the corresponding 3S stereoisomer or as a mixture of the 3R and 3S stereoisomers.
   {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde;   {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde.   
 
         [0233]    Further, the following compounds are examples of compounds of Formula I or a pharmaceutically acceptable salt thereof. The compounds below may also exist as the corresponding 3S stereoisomer or as a mixture of the 3R and 3S stereoisomers.
   {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(3-fluoro-5-toluidino)formaldehyde;   {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde; and   {(3R)-7-(Dimethylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde.   
 
         [0238]    The present disclosure also provides the following compounds, or a pharmaceutically acceptable salt thereof:
   Anilino{7-cyclopropyl-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}formaldehyde;   {(3R)-7-Cyclopropyl-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde;   {(3R)-6-[(p-Chlorophenyl)methyl]-7-cyclopropyl-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde; and   (3R)-7-Cyclopropyl-6-[(1-naphthyl)methyl]-3-(5-phenyl-2H-1,2,4-triazol-3-yl)-1-thia-3a-aza-4-indanone.   
 
         [0243]    There is also provided a pharmaceutical composition comprising a therapeutically effective amount of a compound of the present disclosure such as Formula I as defined herein, any of its stereoisomers, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable adjuvant, diluent or carrier. 
         [0244]    A compound of the present disclosure such as Formula I as described herein, any of its stereoisomers and any mixture of its stereoisomers, or any pharmaceutically acceptable salt thereof, may be used as a medicament in therapy. 
         [0245]    It has been found that compounds of the present disclosure such as of Formula I as described herein are useful in the treatment, prevention and/or alleviation of a bacterial infection such as a  Chlamydia  infection. As used herein, a  Chlamydia  infection is understood to be an infection involving a  Chlamydia  species such as  Chlamydia trachomatis . While not wishing to be bound by any specific theory, it is believed that the compounds of Formula I as described herein block the generation of  Chlamydia trachomatis  progeny by targeting the glucose metabolism of the  Chlamydia trachomatis.    
         [0246]    Accordingly, there is provided a compound as described herein such as a compound of Formula I, any of its stereoisomers, any mixture of its stereoisomers, or a pharmaceutically acceptable salt thereof, for use in the treatment, prevention and/or alleviation of a bacterial infection. The bacterial infection may involve gram-negative and/or gram-positive bacteria. The bacterial infection may be a  Chlamydia  infection. 
         [0247]    There is also provided a use of a compound as described herein such as a compound of Formula I, any of its stereoisomers, any mixture of its stereoisomers, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for use in the treatment, prevention and/or alleviation of a bacterial infection. The bacterial infection may involve gram-negative and/or gram-positive bacteria. The bacterial infection may be a  Chlamydia  infection. 
         [0248]    Further, there is provided a method of treatment, prevention and/or alleviation of a bacterial infection comprising administering to a mammal, including a human, in need thereof an effective amount of a compound as described herein such as a compound of Formula I, any of its stereoisomers, any mixture of its stereoisomers, or pharmaceutically acceptable salt thereof. The bacterial infection may involve gram-negative and/or gram-positive bacterial. The bacterial infection may be a  Chlamydia  infection. 
         [0249]    A suitable pharmaceutically acceptable salt of a compound of the present disclosure may be, for example, an acid-addition salt of a compound of the present disclosure which is sufficiently basic, for example, an acid-addition salt with, for example, an inorganic or organic acid, for example hydrochloric, hydrobromic, nitric, methansulphonic, sulphuric, phosphoric, trifluoroacetic, para-toluene sulphonic, 2-mesitylen sulphonic, citric, acetic, tartaric, fumaric, lactic, succinic, malic, malonic, maleic, 1,2-ethanedisulphonic, adipic, aspartic, benzenesulphonic, benzoic, ethanesulphonic or nicotinic acid. In addition a suitable pharmaceutically acceptable salt of a compound of the present disclosure, is, for example, a base-addition salt of a compound of the present disclosure which is sufficiently acidic, for example, a metal salt, for example, sodium, potassium, calcium, magnesium, zinc or aluminum, an ammonium salt, a salt with an organic base which affords a physiologically acceptable cation, which includes quartenery ammonium hydroxides, for example methylamine, ethylamine, diethylamine, trimethylamine, tert-butylamine, triethylamine, dibenzylamine, N,N-dibenzylethylamine, cyclohexylethylamine, tris-(2-hydroxyethyl)amine, hydroxyethyl diethylamine, (IR, 2S)-2-hydroxyinden-1-amine, morpholine, N-methylpiperidine, N-ethylpiperidine, imidazole, piperazine, methylpiperazine, adamantylamine, choline hydroxide, tetrabutylammonium hydroxide, tris-(hydroxymethyl)methylamine hydroxide, L-arginine, N-methyl D-glucamine, lysine or arginine. 
         [0250]    Certain compounds of the present disclosure may exist as solvates or hydrates. It is to be understood that the present disclosure encompasses all such solvates or hydrates. 
         [0251]    Compounds of the present disclosure may also contain unnatural proportions of atomic isotopes at one or more of the atoms that constitute such compounds. For example, the compounds may be radiolabeled with radioactive isotopes, such as for example tritium ( 3 H), iodine-125 ( 125 I) or carbon-14 ( 14 C). All isotopic variations of the compounds of the present disclosure, whether radioactive or not, are intended to be encompassed within the scope of the present disclosure. 
         [0252]    Compounds of the present disclosure may be administered in the form of a pro-drug. Further, the compounds of the present disclosure may be administered in combination with another therapeutic agent, for instance in the context of the treatment, prevention and/or alleviation of a bacterial infection such as a  Chlamydia  infection. Such a combination may consist or comprise of a compound of the present disclosure and an antibiotic. 
       Methods of Preparation 
       [0253]    Compounds of the present disclosure may be prepared as described in Schemes 1-10. The compounds may also be prepared as described for structurally related compounds. The reactions may be carried out as in standard procedures or as described in the experimental section. 
         [0254]    The 2-pyridone scaffold may be built up by a condensation reaction between the methyl-1,3-thiazoline-4-carboxylate and a Meldrum acid derivative under acidic catalysis at elevated temperature (Scheme 1). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0255]    De-methylation in R 2  using BBr 3  in DCM occurs with partial hydrolysis of the methyl ester. O-alkylation followed by hydrolysis of the mixture of esters gives alkyl ethers on R 2  as their acid derivatives (Scheme 2). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0256]    Nitration in the presence of oxygen directly followed by reduction of the nitro compound gives access to amino substituent in R 4  (Scheme 3). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0257]    Suzuki coupling with boronic acids using Pd catalysis gives access to functionalization in position R 3  (Scheme 4). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0258]    Cleavage of the Cbz protection with HBr/HOAc followed by N-alkylation with alkyl halide or by reductive amination gave the alkyl amines while N-acylation with acyl halides or sufonyl chlorides gave access to carbamates, carboxamides and sulphonamides with different Z 1  and/or Z 2  substituents (Scheme 5). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0259]    Chlorination with NCS in MeCN directly followed by de-bromination using PEPPSI-IPr™ gave chloro in position R 2  (Scheme 6). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0260]    Hydrolysis of the methyl ester with LiOH in THF or LiBr, Et 3 N and wet MeCN gives the carboxylic acids. Amide coupling using T3P, pyridine in MeCN/EtOAc or HATU, DIPEA in DCM gives the corresponding amides (Scheme 7). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0261]    Oxadiazole and triazole were prepared from the carboxylic acids according to Scheme 8. 
         [0000]    
       
                 
         
             
             
         
       
     
         [0262]    Further, the compounds of the present disclosure may be prepared as in Scheme 9 or Scheme 10. 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    
       
                 
         
             
             
         
       
     
         [0263]    Certain intermediates may be used in the synthesis of compounds of the present disclosure such as compounds of Formula I. The present disclosure is directed to such intermediates and/or use thereof in the manufacture of compounds of the present disclosure such as compounds of Formula I. Examples of such intermediates include the following compounds:
   (3R)-7-Hydroxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-7-(Methoxycarbonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-7-Acetylamino-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid;   (3R)-7-(Methylsulfonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid; and   (3R)-7-(Dimethylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid.   
 
         [0271]    Further examples of such intermediates include the following compounds:
   Methyl (3R)-7-methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylate;   Methyl (3R)-6-(chloromethyl)-7-methoxy-4-oxo-1-thia-3a-aza-3-indancarboxylate;   Methyl (3R)-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylate;   Methyl (3R)-7-hydroxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylate; and   Methyl (3R)-6-[(1-naphthyl)methyl]-4-oxo-7-(trifluoromethyl)-1-thia-3a-aza-3-indancarboxylate.   
 
         [0277]    In this document, unless otherwise stated, the naming and the drawing of the chemical compounds and radicals have been made using the program Chem Doodle version 7.0.1 or version 7.0.2. If the name and drawing are inconsistent, the chemical structure shall be considered to be correct. 
         [0278]    The previously mentioned compound 7-(naphthalen-1-ylmethyl)-8-cyclopropyl-5-oxo-N-phenyl-5H-[1,3]thiazolo[3,2-a]pyridine-3-carboxamide (KSK 120) corresponds to the Chem Doodle name 9-Anilinocarbonyl-5-cyclopropyl-4-[(1-naphthyl)methyl]-7-thia-1-azabicyclo[4.3.0]nona-3,5,8-trien-2-one. 
         [0279]    The disclosure is further illustrated by the following non-limitative Examples. 
       EXAMPLES 
     Abbreviations 
       [0000]    
       
         ATCC American Type Culture Collection 
         Bn bensyl 
         Cbz Carboxybenzyl 
         DAPI 4′,6-diamidino-2-phenylindole 
         DCC dicyclocarboimide 
         DCE 1,2-dichloroethane 
         DCM dichloromethane 
         DIPEA diisopropylethylamine 
         DMAP N, N-dimethylaminopyridine 
         DMF dimethylformamide 
         DPPA diphenylphosphoryl azide 
         DMSO dimethyl sulphoxide 
         EBs  Chlamydia trachomatis  elementary bodies 
         ESI-TOFI Electro Spray Ionization—Time of Flight Isochronous 
         FITC Fluorescein isothiocyanate 
         HATU 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5b]pyridinium 3-oxid hexafluorophosphate 
         HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffering agent 
         HPLC High Pressure Liquid Chromatography 
         HRMS High Resolution Mass Spectroscopy 
         IFU infection forming units 
         IUPAC International Union of Pure and Applied Chemistry 
         MOI multiplicity of infection 
         MW Micro Wave 
         NMR Nuclear Magnetic Resonance 
         PEPPSI-IPr [1,3-Bis(2,6-Diisopropylphenyl)imidazol-2-ylidene](3-chloropyridyl)palladium(II) dichloride cas nr 905459-27-0 
         RPMI Roswell Park Memorial Institute medium 
         SPG Succinic acid, Sodium phosphate monobasic monohydrate, Glycine buffer 
         TBTU N,N,N,N′-Tetramethyl-O-(benzotriazol-1-yl)uronium tetrafluoroborate 
         THF tetrahydrofurane 
         TFA trifluoroacetic acid 
         T3P 2,4,6-Tripropyl-1,3,5,2,4,6-trioxatriphosphorinane-2,4,6-trioxide solution 
       
     
       Chemistry 
       [0311]    All reagents and solvents were used as received from commercial suppliers, unless indicated otherwise. Triethylamine, N,N-diisopropylethylamine and pyridine were passed through activated alumina oxide and dried over 3 Å molecular sieves prior to use. CH 2 Cl 2 , THF and DMF were dried in a solvent drying system (CH 2 Cl 2  and THF drying agent: neutral alumina; DMF drying agent: activated molecular sieves equipped with an isocyanate scrubber) and collected fresh prior to every reaction. NaH was prewashed with pentane and dried under vacuum prior to use. Microwave reactions were performed using a Biotage Initiator microwave synthesizer in sealed vessels with temperature monitoring by an internal IR probe. TLC was performed on aluminum backed silica gel plates (median pore size 60 Å) and detected with UV light at 254 nm. Column chromatography was performed using silica gel with average particle diameter 50 μM (range 40-65 μM, pore diameter 53 Å) and eluents are given in brackets. Preparatory HPLC purifications were performed on a system equipped with a 250×21.5 mm Nucleodur® C18 HTEC (particle size 5 μM) column using a flow rate of 20 mL/min and detection at 220 nm. Optical rotation was measured with a polarimeter at 25° C. at 589 nm.  1 H and  13 C NMR spectra were recorded on a 400 or 600 MHz spectrometer at 298 K and calibrated by using the residual peak of the solvent as the internal standard (CDCl 3 : δ H =7.26 ppm; δ C =77.16 ppm; DMSO-d 6 : δ H =2.50 ppm; δ C =39.50 ppm). HRMS was performed by using a mass spectrometer with ESI-TOF (ESI+); sodium formate was used as the calibration chemical. Compounds are named according to IUPAC nomenclature by ChemDoodle version 7.0.1 or version 7.0.2. 
         [0312]    The following Examples were prepared in accordance or in analogy with any one of Schemes disclosed in this document unless otherwise stated. 
       Example 1 
     {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0313]    
       
                 
         
             
             
         
       
     
         [0314]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.66 (dd, J=7.9, 11.2 Hz, 1H), 3.76 (s, 3H), 4.19-4.39 (m, 3H), 5.76 (d, J=7.9 Hz, 1H), 5.79 (s, 1H), 7.05-7.10 (m, 1H), 7.25-7.30 (m, 2H), 7.33 (d, J=7.0 Hz, 1H), 7.41-7.54 (m, 5H), 7.78-7.84 (m, 2H), 7.85-7.90 (m, 1H), 10.33 (s, 1H). 
       Example 2 
     {(3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde 
       [0315]    
       
                 
         
             
             
         
       
     
         [0316]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.28 (s, 3H), 3.62 (dd, J=7.9, 11.2 Hz, 1H), 3.76 (s, 3H), 4.17-4.40 (m, 3H), 5.73 (d, J=7.9 Hz, 1H), 5.78 (s, 1H), 7.03-7.08 (m, 2H), 7.31-7.52 (m, 6H), 7.77-7.91 (m, 3H), 10.23 (s, 1H). 
       Example 3 
     {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0317]    
       
                 
         
             
             
         
       
     
         [0318]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.10 (s, 3H), 2.29 (s, 3H), 3.66 (dd, J=7.9, 11.2 Hz, 1H), 3.72 (s, 3H), 3.86 (dd, J=17.4, 35.7 Hz, 2H), 4.26 (d, J=11.2 Hz, 1H), 5.76-5.81 (m, 2H), 6.93-6.98 (m, 1H), 7.03-7.12 (m, 3H), 7.26-7.32 (m, 2H), 7.52-7.57 (m, 2H), 10.40 (br s, 1H). 
       Example 4 
     {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(3-fluoro-5-toluidino)formaldehyde 
       [0319]    
       
                 
         
             
             
         
       
     
         [0320]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.10 (s, 3H), 2.27 (s, 3H), 2.29 (s, 3H), 3.65 (dd, J=7.9, 11.3 Hz, 1H), 3.72 (s, 3H), 3.86 (dd, J=17.3, 35.8 Hz, 2H), 4.20 (d, J=11.3 Hz, 1H), 5.76 (dd, J=7.9 Hz, 1H), 5.79-5.77 (m, 1H), 6.57-6.61 (m, 1H), 6.94-6.97 (m, 2H), 7.04-7.10 (m, 2H), 7.31-7.36 (m, 1H), 10.48 (bs, 1H). 
       Example 5 
     {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde 
       [0321]    
       
                 
         
             
             
         
       
     
         [0322]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.10 (s, 3H), 2.29 (s, 3H), 2.30 (s, 3H), 3.64 (dd, J=7.9, 11.1 Hz, 1H), 3.72 (s, 3H), 3.86 (dd, J=17.3, 33.9 Hz, 2H), 4.21 (d, J=11.1 Hz, 1H), 5.72-5.80 (m, 2H), 6.94-6.98 (m, 1H), 7.03-7.11 (m, 4H), 7.39-7.45 (m, 2H), 10.28 (bs, 1H). 
       Example 6 
     {(3R)-5-Amino-7-methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0323]    
       
                 
         
             
             
         
       
     
         [0324]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.59 (s, 3H), 2.67 (dd, J=7.7, 11.2 Hz, 1H), 3.99 (bs, 2H), 4.20 (d, J=11.2 Hz, 1H), 4.34 (dd, J=16.5, 29.0 Hz, 2H), 5.85 (d, J=7.7 Hz, 1H), 7.07-7.16 (m, 2H), 7.28-7.39 (m, 3H), 7.53-7.63 (m, 4H), 7.77 (d, J=8.3 Hz, 1H), 7.88-7.92 (m, 1H), 8.17 (d, J=8.3 Hz, 1H), 10.4 (bs, 1H). 
       Example 7 
     {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde 
       [0325]    
       
                 
         
             
             
         
       
     
         [0326]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.29 (s, 3H), 2.30 (s, 3H), 2.32 (s, 3H), 3.57 (s, 3H), 3.66 (dd, J=7.7, 11.2 Hz, 1H), 3.75-4.05 (m, 4H), 4.20 (d, J=11.2 Hz, 1H), 5.84 (d, J=7.7 Hz, 1H), 6.79 (d, J=7.4 Hz, 1H), 6.99 (t, J=7.4 Hz, 1H), 7.06 (d, J=7.4 Hz, 1H), 7.11 (d, J=8.2 Hz, 2H), 7.46 (d, J=8.2 Hz, 2H), 10.29 (br s, 1H). 
       Example 8 
       [0327]    {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(3-fluoro-5-toluidino)formaldehyde 
         [0000]    
       
                 
         
             
             
         
       
     
         [0328]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.29 (s, 3H), 2.30 (s, 3H), 2.32 (s, 3H), 3.57 (s, 3H), 3.66 (dd, J=7.7, 11.3 Hz, 1H), 3.78-3.99 (m, 4H), 4.17 (d, J=11.3 Hz, 1H), 5.82 (d, J=7.7 Hz, 1H), 6.61 (d, J=9.3 Hz, 1H), 6.77-6.81 (m, 1H), 6.95-7.08 (m, 3H), 7.30-7.36 (m, 1H), 10.49 (br s, 1H). 
       Example 9 
     {(3R)-5-Amino-7-methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde 
       [0329]    
       
                 
         
             
             
         
       
     
         [0330]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.31 (s, 3H), 3.58 (s, 3H), 3.67 (dd, J=7.7, 11.2 Hz, 1H), 3.98 (br s, 2H), 4.20 (d, J=11.2 Hz, 1H), 4.34 (dd, J=16.5, 29.1 Hz, 2H), 5.84 (d, J=7.7 Hz, 1H), 7.09.7.16 (m, 3H), 7.33-7.39 (m, 1H), 7.45-7.50 (m, 2H), 7.52-7.62 (m, 2H), 7.77 (d, J=8.2 Hz, 1H), 7.90 (d, J=7.9 Hz, 1H), 8.17 (d, J=8.3 Hz, 1H), 10.29 (br s, 1H). 
       Example 10 
     {(3R)-7-Hydroxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0331]    
       
                 
         
             
             
         
       
     
         [0332]      1 H-NMR, 400 MHz, (DMSO) δ 3.57 (dd, J 1.9, 12.0 Hz, 1H), 3.9 (dd, J=8.9, 12.0 Hz, 1H), 4.27 (dd, J=16.8, 34.3 Hz, 2H), 5.27 (s, 1H), 5.48 (dd, J=1.9, 8.9 Hz, 1H), 7.03-7.08 (m, 1H), 7.27-7.33 (m, 2H), 7.42 (d, J=6.9 Hz, 1H), 7.48-7.57 (m, 5H), 7.85-7.99 (m, 3H), 8.60 (s br, 1H), 10.39 (s, 1H). 
       Example 11 
     {(3R)-7-Chloro-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0333]    
       
                 
         
             
             
         
       
     
         [0334]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.70 (dd, J=8.0, 11.3 Hz, 1H), 4.20-4.39 (m, 3H), 5.75 (s, 1H), 5.81 (d, J=8.0 Hz, 1H), 7.04-7.10 (m, 1H), 7.23-7.33 (m, 3H), 7.42-7.53 (m, 5H), 7.67-7.75 (m, 1H), 7.80-7.92 (m, 2H), 10.01 (s, 1H). 
       Example 12 
     {(3R)-6-[(1-Naphthyl)methyl]-4-oxo-7-(trifluoromethyl)-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0335]    
       
                 
         
             
             
         
       
     
         [0336]      1 H-NMR, 400 MHz, (DMSO-d 6 ) δ 3.66 (dd, J=1.4, 12.0 Hz, 1H), 3.99 (dd, J=9.4, 12.0 Hz, 1H), 4.40 (s, 2H), 5.40 (s, 1H), 5.59 (d, J=9.4 Hz, 1H), 7.05-7.10 (m, 1H), 7.28-7.34 (m, 2H), 7.40-7.43 (m, 1H), 7.50-7.59 (m, 5H), 7.76-7.81 (m, 1H), 7.89-7.94 (m, 1H), 7.96-8.01 (m, 1H), 10.51 (bs, 1H). 
       Example 13 
     {(3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0337]    
       
                 
         
             
             
         
       
     
         [0338]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.29 (s, 3H), 2.33 (s, 3H), 3.57 (s, 3H), 3.66 (dd, J=7.7, 11.2 Hz, 1H), 3.78-4.01 (m, 4H), 4.19 (d, J=11.2 Hz, 1H), 5.85 (d, J=7.7 Hz, 1H), 6.80 (d, J=7.5 Hz, 1H), 6.96-7.13 (m, 3H), 7.27-7.34 (m, 2H), 7.55-7.61 (m, 2H), 10.41 (bs, 1H). 
       Example 14 
     (3R)-5-Amino-7-methoxy-3-(3-phenyl-1,2,4-oxadiazol-5-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone 
       [0339]    
       
                 
         
             
             
         
       
     
         [0340]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.30 (s, 3H), 2.33 (s, 3H), 3.59 (s, 3H), 3.73 (dd, J=1.8, 11.8 Hz, 1H), 3.81-4.03 (m, 5H), 6.47 (dd, J=1.8, 7.5 Hz, 1H), 6.86 (d, J=7.5, 1H), 6.98-7.09 (m, 2H), 7.43-7.53 (m, 3H), 8.04-8.09 (m, 2H). 
       Example 15 
     (3R)-7-Methoxy-3-(5-phenyl-1,3,4-oxadiazol-2-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone 
       [0341]    
       
                 
         
             
             
         
       
     
         [0342]      1 H-NMR, 400 MHz, (DMSO-d 6 ) δ 2.09 (s, 3H), 2.26 (s, 3H), 3.70 (s, 3H), 3.84-3.89 (m, 3H), 4.09 (dd, J=8.2, 11.9 Hz, 1H), 5.39-5.40 (m, 1H), 6.42 (dd, J=1.9, 8.2 Hz, 1H), 6.99-7.12 (m, 3H), 7.58-7.68 (m, 3H), 7.93-7.99 (m, 2H). 
       Example 16 
     (3R)-7-Methoxy-3-(3-phenyl-1,2,4-oxadiazol-5-yl)-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-4-indanone 
       [0343]    
       
                 
         
             
             
         
       
     
         [0344]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.13 (s, 3H), 2.30 (s, 3H), 3.72-3.76 (m, 4H), 3.89 (dd, J=17.2, 20.3 Hz, 2H), 3.97 (dd, J=7.8, 11.8 Hz, 1H), 5.75 (t, J=1.1 Hz, 1H), 6.40 (dd, J=1.7, 7.8 Hz, 1H), 6.97-7.11 (m, 3H), 7.43-7.53 (m, 3H), 8.02-8.06 (m, 2H). 
       Example 17 
     {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde 
       [0345]    
       
                 
         
             
             
         
       
     
         [0346]      1 H NMR (400 MHz, CDCl 3 ) δ=2.22 (s, 3H), 2.29 (s, 3H), 3.71 (dd, J=7.6, 11.2 Hz, 1H), 3.76 (s, 3H), 4.20 (d, J=11.2 Hz, 1H), 4.91-5.00 (m, 2H), 5.88 (d, J=7.6 Hz, 1H), 6.68 (s, 1H), 6.71 (d, J=8.0 Hz, 1H), 6.82 (d, J=7.6 Hz, 1H), 7.04 (dd, J=7.6, 8.0 Hz, 1H), 7.63 (dd, J=2.4, 8.8 Hz, 1H), 8.10 (d, J=8.8 Hz, 1H), 8.25 (d, J=2.4 Hz, 1H), 10.82 (s, 1H). 
       Example 18 
     {(3R)-7-Methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indanyl}(5-chloro-2-pyridylamino)formaldehyde 
       [0347]    
       
                 
         
             
             
         
       
     
         [0348]      1 H NMR (400 MHz, CDCl 3 ) δ=2.23 (s, 3H), 2.30 (s, 3H), 3.68 (dd, J=7.6, 11.2 Hz, 1H), 3.76 (s, 3H), 4.29 (d, J=11.2 Hz, 1H), 4.92-5.01 (m, 2H), 5.85 (d, J=7.6 Hz, 1H), 6.62 (s, 1H), 6.72 (d, J=8.0 Hz, 1H), 6.83 (d, J=7.6 Hz, 1H), 7.05 (dd, J=7.6, 8.0 Hz, 1H), 7.10 (d, J=7.6 Hz, 1H), 7.30 (dd, J=7.6, 8.4 Hz, 2H), 7.57 (d, J=8.4 Hz, 2H), 10.37 (s, 1H). 
       Example 19 
     Anilino{7-cyclopropyl-4-oxo-6-[(2,3-xylylyloxy)methyl]-1-thia-3a-aza-3-indanyl}formaldehyde 
       [0349]    
       
                 
         
             
             
         
       
     
         [0350]      1 H NMR (400 MHz, CDCl 3 ) δ=0.55-0.63 (m, 1H), 0.68-0.78 (m, 1H), 0.85-1.03 (m, 2H), 1.60-1.68 (m, 1H), 2.24 (s, 3H), 2.30 (s, 3H), 3.59 (dd, J=8.0, 11.2 Hz, 1H), 4.20 (d, J=11.2 Hz, 1H), 5.06-5.09 (m, 2H), 5.86 (d, J=7.9 Hz, 1H), 6.66-6.70 (m, 2H), 6.83 (d, J=7.6 Hz, 1H), 7.02-7.10 (m, 2H), 7.25-7.32 (m, 2H), 7.53-7.58 (m, 2H), 10.31 (br s, 1H). 
       Example 20 
     {(3R)-7-Cyclopropyl-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde 
       [0351]    
       
                 
         
             
             
         
       
     
         [0352]      1 H NMR (400 MHz, CDCl 3 ) δ=0.57-0.66 (m, 1H), 0.71-0.80 (m, 1H), 0.85-0.95 (m, 1H), 0.97-1.01 (m, 1H), 1.58-1.68 (m, 1H), 2.07 (s, 3H), 2.28 (s, 3H), 2.30 (s, 3H), 3.55 (dd, J=8.0, 11.2 Hz, 1H), 3.92-4.04 (m, 2H), 4.17 (d, J=11.2 Hz, 1H), 5.76-5.80 (m, 2H), 6.89-6.92 (m, 1H), 7.03-7.11 (m, 4H), 7.39-7.44 (m, 2H), 10.23 (s, 1H). 
       Example 21 
     {(3R)-6-[(p-Chlorophenyl)methyl]-7-cyclopropyl-4-oxo-1-thia-3a-aza-3-indanyl}(p-toluidino)formaldehyde 
       [0353]    
       
                 
         
             
             
         
       
     
         [0354]      1 H NMR (400 MHz, CDCl 3 ) δ=0.52-0.61 (m, 1H), 0.65-0.74 (m, 1H), 0.79-0.89 (m, 1H), 0.91-1.01 (m, 1H), 1.36-1.45 (m, 1H), 2.29 (s, 3H), 3.55 (dd, J=8.0, 11.2 Hz, 1H), 3.90 (d, J=16.0 Hz, 1H), 4.00 (d, J=16.0 Hz, 1H), 4.16 (d, J=11.2 Hz, 1H), 5.81 (d, 7.9, 1H), 6.05 (s, 1H), 7.06-7.12 (m, 4H), 7.25-7.30 (m, 2H), 7.40-7.45 (m, 2H), 10.20 (s, 1H). 
       Example 22 
     (3R)-7-Cyclopropyl-6-[(1-naphthyl)methyl]-3-(5-phenyl-2H-1,2,4-triazol-3-yl)-1-thia-3a-aza-4-indanone 
       [0355]    
       
                 
         
             
             
         
       
     
         [0356]      1 H NMR (400 MHz, DMSO-d 6 ) δ=0.62-0.71 (m, 1H), 0.74-0.82 (m, 1H), 0-84-0.99 (m, 2H), 1-70-1.79 (m, 1H), 3.50 (dd, J=0.9, 11.6 Hz, 1H), 3.96 (dd, J=8.1, 11.6 Hz, 1H), 4.39-4.55 (m, 2H), 5.30 (s, 1H), 6.14 (dd, J=0.9, 8.0 Hz, 1H), 7.41 (d, J=6.5 Hz, 1H), 7.44-7.59 (m, 6H), 7.85-8.00 (m, 5H), 14.21 (br s, 1H). 
       Example 23 
     Anilino{7-(isopropylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}formaldehyde 
       [0357]    
       
                 
         
             
             
         
       
     
         [0358]      1 H NMR (400 MHz, DMSO-d 6 ) δ 10.36 (s, 1H), 8.03-7.92 (m, 1H), 7.90-7.76 (m, 2H), 7.63-7.47 (m, 5H), 7.43 (dd, J=7.1, 1.3 Hz, 1H), 7.30 (dd, J=8.5, 7.3 Hz, 2H), 7.15-6.99 (m, 1H), 5.48 (dd, J=9.0, 2.1 Hz, 1H), 5.16 (s, 1H), 4.42 (d, J=17.1 Hz, 1H), 4.29 (d, J=17.1 Hz, 1H), 3.87 (dd, J=11.9, 9.0 Hz, 1H), 3.65-3-60 (m, 1H), 3.50 (dd, J=11.9, 2.1 Hz, 1H), 1.22-1.02 (m, 6H). 
       Example 24 
     {(3R)-7-(Dimethylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0359]    
       
                 
         
             
             
         
       
     
         [0360]      1 H NMR (400 MHz, DMSO-d 6 ) δ 10.39 (s, 1H), 7.98-7.94 (m, 1H), 7.93-7.89 (m, 1H), 7.86 (d, J=8.2 Hz, 1H), 7.60-7.46 (m, 5H), 7.40 (d, J=7.1 Hz, 1H), 7.37-7.23 (m, 2H), 7.09-7.02 (m, 1H), 5.45 (dd, J=9.2, 2.4 Hz, 1H), 5.30 (s, 1H), 4.35 (d, J=16.7 Hz, 1H), 4.30 (d, J=16.8 Hz, 1H), 3.92 (dd, J=11.9, 9.2 Hz, 1H), 3.55 (dd, J=12.0, 2.4 Hz, 1H), 2.71 (s, 6H). 
       Example 25 
     Anilino{7-(methylsulfonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}formaldehyde 
       [0361]    
       
                 
         
             
             
         
       
     
         [0362]      1 H NMR (400 MHz, DMSO-d 6 ) δ 10.41 (s, 1H), 9.29 (s, br, 1H), 8.01-7.94 (m, 1H), 7.93-7.84 (m, 2H), 7.56-7.47 (m, 5H), 7.43 (d, J=7.1 Hz, 1H), 7.35-7.24 (m, 2H), 7.14-6.85 (m, 1H), 5.50 (dd, J=9.1, 2.1 Hz, 1H), 5.03 (s, 1H), 4.47 (d, J=17.7 Hz, 1H), 4.31 (d, J=17.6 Hz, 1H), 4.02 (dd, J=11.9, 9.2 Hz, 1H), 3.60 (dd, J=11.9, 2.1 Hz, 1H), 3.21 (s, 3H). 
       Example 26 
     1-{3-Anilinocarbonyl-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-7-indanylamino}-1-ethanone 
       [0363]    
       
                 
         
             
             
         
       
     
         [0364]      1 H NMR (400 MHz, DMSO-d 6 ) δ 10.18 (s, 1H), 9.18 (s, 1H), 8.00-7.71 (m, 3H), 7.58-7.45 (m, 5H), 7.38 (d, J=7.0 Hz, 1H), 7.35-7.23 (m, 2H), 7.12-6.96 (m, 1H), 5.54 (dd, J=9.1, 2.8 Hz, 1H), 5.25 (s, 1H), 4.16 (d, J=17.2 Hz, 1H), 4.11 (d, J=17.2 Hz, 1H), 3.90 (dd, J=11.9, 9.0 Hz, 1H), 3.56 (dd, J=11.8, 2.8 Hz, 1H), 2.01 (s, 3H). 
       Example 27 
     {(3R)-7-(Methoxycarbonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indanyl}anilinoformaldehyde 
       [0365]    
       
                 
         
             
             
         
       
     
         [0366]      1 H NMR (400 MHz, DMSO-d 6 ) δ 10.41 (s, 1H), 8.90 (s, 1H), 8.02-7.95 (m, 1H), 8.19-7.77 (m, 1H), 7.86 (d, J=7.7 Hz, 1H), 7.53 (dt, J=10.4, 7.8 Hz, 5H), 7.42 (d, J=7.2 Hz, 1H), 7.31 (t, J=7.8 Hz, 2H), 7.07 (t, J=7.4 Hz, 1H), 5.51 (dd, J=9.2, 2.3 Hz, 1H), 5.15 (s, 1H), 4.18 (d, J=17.2 Hz, 1H), 4.12 (d, J=17.0 Hz, 1H), 3.96 (dd, J=11.8, 9.2 Hz, 1H), 3.65 (s, 3H), 3.57 (dd, J=12.0, 2.4 Hz, 1H). 
       Example 28 
     (3R)-7-(Cyclopropylmethoxy)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0367]    
       
                 
         
             
             
         
       
     
         [0368]      1 H-NMR, 400 MHz, (DMSO) δ 0.24-0.30 (m, 2H), 0.51-0.57 (m, 2H), 1.11-1.24 (m, 1H), 3.57 (dd, J=1.7, 11.9 Hz, 1H), 3.71 (dABq, J=7.2, 14.5 Hz, 2H), 3.87 (dd, J=8.9, 11.9 Hz, 1H), 4.31 (dd, J=16.6, 28.4 Hz, 2H), 5.31-5.35 (m, 2H), 7.41-7.45 (m, 1H), 7.47-7.57 (m, 3H), 7.85-7.99 (m, 3H). 
       Example 29 
     (3R)-6-[(1-Naphthyl)methyl]-4-oxo-7-(trifluoromethyl)-1-thia-3a-aza-3-indancarboxylic acid 
       [0369]    
       
                 
         
             
             
         
       
     
         [0370]      1 H-NMR, 400 MHz, (DMSO) δ 3.62 (dd, J=1.2, 11.9 Hz, 1H), 3.88 (dd, J=9.2, 11.9 Hz, 1H), 4.38 (s, 2H), 5.40 (s, 1H), 5.46 (d, J=9.2 Hz, 1H), 7.39-7.42 (m, 1H), 7.49-7.58 (m, 3H), 7.74-7.80 (m, 1H), 7.89-7.94 (m, 1H), 7.96-8.01 (m, 1H). 
       Example 30 
     (3R)-7-Isobutoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0371]    
       
                 
         
             
             
         
       
     
         [0372]      1 H-NMR, 400 MHz, (DMSO) δ 0.92-0.96 (m, 6H), 1.88-1.99 (m, 1H), 3.52-3.61 (m, 2H), 3.68 (dd, J=6.4, 8.6 Hz, 1H), 3.87 (dd, J=8.9, 11.9 Hz, 1H), 4.29 (dd, J=16.9, 23.5 Hz, 2H), 5.33 (dd, J=1.5, 8.9 Hz, 1H), 5.36 (s, 1H), 7.41-7.44 (m, 1H), 7.48-7.57 (m, 3H), 7.86-7.98 (m, 3H). 
       Example 31 
     (3R)-7-(Dimethylamino)-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylic acid 
       [0373]    
       
                 
         
             
             
         
       
     
         [0374]      1 H-NMR, 400 MHz, (DMSO) δ 2.06 (s, 3H), 2.26 (s, 3H), 2.65 (s, 6H), 3.51 (dd, J=1.9, 11.9 Hz, 1H), 3.79-3.89 (m, 3H), 5.28 (s, 1H), 5.33 (dd, J=1.9, 9.1 Hz, 1H), 6.95-7.00 (m, 1H), 7.02-7.10 (m, 2H). 
       Example 32 
     (3R)-7-(Dimethylamino)-6-[(4-methyl-1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0375]    
       
                 
         
             
             
         
       
     
         [0376]      1 H-NMR, 400 MHz, (DMSO) δ 2.65 (s, 3H), 2.71 (s, 6H), 3.52 (dd, J=1.9, 11.9 Hz, 1H), 3.84 (dd, J=9.2, 11.9 Hz, 1H), 4.27 (dd, J=16.9, 24.5 Hz, 2H), 5.26 (s, 1H), 5.31 (dd, J=1.9, 9.2 Hz, 1H), 7.26-7.29 (m, 1H), 7.33-7.36 (m, 1H), 7.51-7.59 (m, 2H), 7.84-7.90 (m, 1H), 8.03-8.08 (m, 1H). 
       Example 33 
     (3R)-7-(Isopropylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0377]    
       
                 
         
             
             
         
       
     
         [0378]      1 H NMR (400 MHz, Methanol-d 4 ) δ 7.97-7.91 (m, 1H), 7.88 (d, J=8.3 Hz, 1H), 7.86-7.78 (m, 1H), 7.57-7.47 (m, 3H), 7.42 (d, J=7.0 Hz, 1H), 5.66 (dd, J=8.6, 1.5 Hz, 1H), 5.64 (s, 1H), 4.50 (d, J=17.3 Hz, 1H), 4.41 (d, J=17.3 Hz, 1H), 3.98 (dd, J=11.9, 8.6 Hz, 1H), 3.77 (dd, J=11.9, 1.6 Hz, 1H), 3.69 (p, J=6.5 Hz, 1H), 1.34 (t, J=6.1 Hz, 6H). 
       Intermediates 
       [0379]    The following intermediates were prepared as described in this document unless stated otherwise, and used in the synthesis of the compounds of the present disclosure. 
       Intermediate 1 
     (3R)-7-Methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0380]    
       
                 
         
             
             
         
       
     
         [0381]      1 H-NMR, 400 MHz, (DMSO) δ 3.60 (dd, J=1.7, 11.9 Hz, 1H), 3.70 (s, 3H), 3.91 (dd, J=8.9, 11.9 Hz, 1H), 4.30 (dd, J=16.8, 22.3 Hz, 2H), 5.35 (dd, J=1.7, 8.9 Hz, 1H), 5.37-5.38 (m, 1H), 7.41-7.45 (m, 1H), 7.48-7.57 (m, 3H), 7.85-7.99 (m, 3H), 13.43 (br s, 1H). 
       Intermediate 2 
     (3R)-7-Hydroxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0382]    
       
                 
         
             
             
         
       
     
         [0383]      1 H-NMR, 400 MHz, (DMSO) δ 3.53 (dd, J=1.6, 11.9 Hz, 1H), 3.83 (dd, J=8.9, 11.9 Hz, 1H), 4.26 (dd, J=16.9, 34.6 Hz, 2H), 5.27 (s, 1H), 5.33 (dd, J=1.6, 8.9 Hz, 1H), 7.41 (d, J=6.7 Hz, 1H), 7.46-7.57 (m, 3H), 7.84-7.99 (m, 3H), 8.56 (s, 1H), 13.32 (br s, 1H). 
       Intermediate 3 
     (3R)-7-Methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylic acid 
       [0384]    
       
                 
         
             
             
         
       
     
         [0385]      1 H-NMR, 400 MHz, (DMSO) δ 2.07 (s, 3H), 2.26 (s, 3H), 3.60 (dd, J=1.7, 11.9 Hz, 1H), 3.66 (s, 3H), 3.84 (s, 2H), 3.90 (dd, J=8.9, 11.9 Hz, 1H), 5.32-5.34 (m, 1H), 5.37 (dd, J=1.7, 8.9 Hz, 1H), 6.98-7.12 (m, 3H), 13.45 (br s, 1H). 
       Intermediate 4 
     (3R)-5-Amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylic acid 
       [0386]    
       
                 
         
             
             
         
       
     
         [0387]      1 H-NMR, 400 MHz, (DMSO) δ 2.25 (s, 3H), 2.27 (s, 3H), 3.38 (s, 3H), 3.55 (dd, J=1.6, 11.9 Hz, 1H), 3.75 (dd, J=16.3, 21.3 Hz, 2H), 3.85 (dd, J=8.5, 11.9 Hz, 1H), 5.48 (dd, J=1.6, 8.5 Hz, 1H), 6.63 (dd, J=7.4 Hz, 1H), 6.90-7.02 (m, 2H). 
       Intermediate 5 
     7-Cyclopropyl-4-oxo-6-[(2,3-xylyloxy)methyl]-1,3a-diaza-3-indancarboxylic acid 
       [0388]    
       
                 
         
             
             
         
       
     
         [0389]      1 H NMR (400 MHz, DMSO-d 6 ) δ=0.52-0.59 (m, 1H), 0.63-0.70 (m, 1H), 0.81-0.92 (m, 2H), 1.64-1.73 (m, 1H), 3.02 (s, 3H), 3.04 (s, 3H), 3.53 (dd, J=1.6, 11.9 Hz, 1H), 3.81 (dd, J=9.2, 11.9 Hz, 1H), 5.14 (s, 2H), 5.42 (dd, J=1.6, 9.1 Hz), 6.20 (s, 1H), 6.78-6.84 (m, 2H), 7.02-7.09 (m, 1H), 13.41 (br s, 1H). 
       Intermediate 6 
     (3R)-7-(Methoxycarbonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0390]    
       
                 
         
             
             
         
       
     
         [0391]      1 H NMR (400 MHz, Methanol-d 4 ) δ 7.96-7.86 (m, 2H), 7.82 (d, J=8.2 Hz, 1H), 7.52-7.43 (m, 3H), 7.39 (d, J=6.9 Hz, 1H), 5.49 (s, 1H), 5.38 (dd, J=8.6, 1.6 Hz, 1H), 4.23 (s, 2H), 3.83 (dd, J=11.4, 8.6 Hz, 1H), 3.74 (s, br, 3H), 3.63 (dd, J=11.4, 1.6 Hz, 1H). 
       Intermediate 7 
     (3R)-7-Acetylamino-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0392]    
       
                 
         
             
             
         
       
     
         [0393]      1 H NMR (400 MHz, Methanol-d 4 ) δ 7.95-7.86 (m, 2H), 7.82 (d, J=8.2 Hz, 1H), 7.52-7.43 (m, 3H), 7.37 (d, J=6.5 Hz, 1H), 5.38 (dd, J=8.6, 1.6 Hz, 1H), 4.20 (s, 2H), 3.83 (dd, J=11.3, 8.6 Hz, 1H), 3.63 (dd, J=11.3, 1.7 Hz, 1H), 2.08 (s, 3H). 
       Intermediate 8 
     (3R)-7-(Methylsulfonylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0394]    
       
                 
         
             
             
         
       
     
         [0395]    H NMR (400 MHz, Methanol-d 4 ) δ 7.99-7.71 (m, 3H), 7.56-7.29 (m, 4H), 5.56 (d, J=8.7 Hz, 1H), 5.39 (s, 1H), 4.56 (d, J=17.8 Hz, 1H), 4.45 (d, J=17.9 Hz, 1H), 3.96 (dd, J=11.9, 8.8 Hz, 1H), 3.69 (dd, J=11.8, 1.7 Hz, 1H), 3.24 (s, 3H). 
       Intermediate 9 
     (3R)-7-(Dimethylamino)-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0396]    
       
                 
         
             
             
         
       
     
         [0397]      1 H NMR (400 MHz, Methanol-d 4 ) δ 7.96-7.87 (m, 2H), 7.84 (d, J=8.2 Hz, 1H), 7.57-7.46 (m, 3H), 7.40 (d, J=6.9 Hz, 1H), 5.69 (s, 1H), 5.60 (d, J=8.7 Hz, 1H), 4.45 (s, 2H), 3.92 (dd, J=12.0, 8.8 Hz, 1H), 3.69 (d, J=12.0 Hz, 1H), 2.70 (s, 6H). 
       Intermediate 10 
     6-[(p-Chlorophenyl)methyl]-7-cyclopropyl-4-oxo-1,3a-diaza-3-indancarboxylic acid 
       [0398]    
       
                 
         
             
             
         
       
     
         [0399]    Intermediate 17 was prepared according to WO2014185853. 
       Intermediate 11 
     7-Cyclopropyl-4-oxo-6-[(2,3-xylyl)methyl]-1,3a-diaza-3-indancarboxylic acid 
       [0400]    
       
                 
         
             
             
         
       
     
         [0401]    Intermediate 18 was prepared according to WO2014185853. 
       Intermediate 12 
     Methyl (3R)-7-methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indancarboxylate 
       [0402]    
       
                 
         
             
             
         
       
     
       Intermediate 13 
     (3R)-7-Methoxy-4-oxo-6-[(2,3-xylyloxy)methyl]-1-thia-3a-aza-3-indancarboxylic acid 
       [0403]    
       
                 
         
             
             
         
       
     
       Intermediate 14 
     Methyl (3R)-7-chloro-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylate 
       [0404]    
       
                 
         
             
             
         
       
     
         [0405]      1 H NMR (400 MHz, Chloroform-d) δ 7.91-7.86 (m, 1H), 7.81 (d, J=8.2 Hz, 1H), 7.78-7.72 (m, 1H), 7.59-7.37 (m, 3H), 7.31 (d, J=6.7 Hz, 1H), 5.69 (s, 1H), 5.60 (dd, J=8.5, 2.1 Hz, 1H), 4.36 (d, J=17.5 Hz, 1H), 4.24 (d, J=17.6 Hz, 1H), 3.80 (s, 3H), 3.79 (dd, J=11.9, 8.6 Hz, 1H), 3.59 (dd, J=11.8, 2.2 Hz, 1H). 
       Intermediate 15 
     (3R)-7-Chloro-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylic acid 
       [0406]    
       
                 
         
             
             
         
       
     
         [0407]      1 H NMR (400 MHz, DMSO-d 6 ) δ 8.01-7.95 (m, 1H), 7.94-7.87 (m, 2H), 7.60-7.47 (m, 3H), 7.35 (d, J=7.1 Hz, 1H), 5.51 (s, 1H), 5.47 (dd, J=9.1, 1.7 Hz, 1H), 4.36 (d, J=17.2 Hz, 1H), 4.30 (d, J=17.1 Hz, 1H), 3.97 (dd, J=12.0, 9.1 Hz, 1H), 3.64 (dd, J=11.9, 1.7 Hz, 1H). 
       Intermediate 16 
     Methyl (3R)-7-methoxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylate 
       [0408]    
       
                 
         
             
             
         
       
     
         [0409]      1 H-NMR (400 MHz, CDCl 3 ) δ 3.57 (dd, J=2.4, 12.0 Hz, 1H), 3.73 (dd, J=8.8, 11.6 Hz, 1H), 3.74 (s, 3H), 3.78 (s, 1H), 4.28 (q, J=17.2 Hz, 2H), 5.53 (dd, J=2.4 Hz, 8.4 Hz, 1H), 5.72 (s, 1H), 7.33 (d, J=6.8 Hz, 1H), 7.40-7.49 (m, 3H), 7.78 (d, J=8.4 Hz, 1H), 7.82-7.87 (m, 2H). 
       Intermediate 17 
     Methyl (3R)-6-(chloromethyl)-7-methoxy-4-oxo-1-thia-3a-aza-3-indancarboxylate 
       [0410]    
       
                 
         
             
             
         
       
     
         [0411]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.61 (dd, J=2.3, 11.7 Hz, 1H), 3.73-3.80 (m, 4H), 3.82 (s, 3H), 4.37-4.45 (m, 2H), 5.59 (dd, J=2.3, 8.4 Hz, 1H), 6.36-6.37 (m, 1H). 
       Intermediate 18 
     Methyl (3R)-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylate 
       [0412]    
       
                 
         
             
             
         
       
     
         [0413]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.10 (s, 3H), 2.28 (s, 3H), 3.58 (dd, J=2.3, 11.7 Hz, 1H), 3.71 (s, 3H), 3.75 (dd, J=8.4, 11.7 Hz, 1H), 3.79-3.91 (m, 5H), 5.56 (dd, J=2.3, 8.4 Hz, 1H), 5.71 (t, J=1.1 Hz, 1H), 6.94-7.09 (m, 3H). 
       Intermediate 19 
     Methyl (3R)-7-hydroxy-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylate 
       [0414]    
       
                 
         
             
             
         
       
     
         [0415]      1 H-NMR, 400 MHz, (DMSO) δ 3.56 (dd, J=2.0, 12.0 Hz, 1H), 3.68 (s, 3H), 3.86 (dd, J=8.9, 12.0 Hz, 1H), 4.27 (dd, J=17.0, 36.5 Hz, 2H), 5.29 (s, 1H), 5.44 (dd, J=2.0, 8.9 Hz, 1H), 7.39-7.56 (m, 4H), 7.85-7.99 (m, 3H), 8.61 (s, 1H). 
       Intermediate 20 
     Methyl (3R)-6-[(1-naphthyl)methyl]-4-oxo-7-(trifluoromethyl)-1-thia-3a-aza-3-indancarboxylate 
       [0416]    
       
                 
         
             
             
         
       
     
         [0417]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.52 (dd, J=2.0, 11.9 Hz, 1H), 3.67 (dd, J=8.7, 11.9 Hz, 1H), 3.79 (m, 3H), 4.36 (dd, J=18.0, 39.5 Hz, 1H), 5.55-5.60 (m, 1H), 5.64 (s, 1H), 7.31-7.36 (m, 1H), 7.41-7.52 (m, 3H), 7.66-7.72 (m, 1H), 7.79-7.90 (m, 2H). 
       Intermediate 21 
     Methyl (3R)-5-amino-7-methoxy-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylate 
       [0418]    
       
                 
         
             
             
         
       
     
         [0419]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.28 (s, 3H), 2.31 (s, 3H), 3.55 (s, 3H), 3.57 (dd, J=2.3, 11.7 Hz, 1H), 3.74 (dd, J=8.0, 11.7 Hz, 1H), 3.78-3.90 (m, 5H), 5.63 (dd, J=2.3, 8.0 Hz, 1H), 6.78-6.83 (m, 1H), 6.95-7.06 (m, 2H). 
       Intermediate 22 
     Methyl (3R)-7-(benzyloxycarbonylamino)-4-oxo-6-[(2,3-xylyl)methyl]-1-thia-3a-aza-3-indancarboxylate 
       [0420]    
       
                 
         
             
             
         
       
     
         [0421]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.06 (s, 3H), 2.27 (s, 3H), 3.50-3.85 (m, 7H), 5.19 (s, 2H), 5.59 (dd, J=2.3, 8.5 Hz, 1H), 5.72-5.82 (m, 2H), 6.80-7.10 (m, 3H), 7.28-7.43 (m, 5H). 
       Intermediate 23 
     Methyl (3R)-7-(benzyloxycarbonylamino)-6-[(4-methyl-1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylate 
       [0422]    
       
                 
         
             
             
         
       
     
         [0423]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 2.68 (s, 3H), 3.54 (dd, J=2.1, 11.7 Hz, 1H), 3.66-3.83 (m, 4H), 4.15 (dd, J=17.2, 41.0 Hz, 2H), 5.17 (s, 2H), 5.57 (dd, J=2.1, 8.5 Hz, 1H), 5.67-5.90 (m, 2H), 7.05-7.25 (m, 2H), 7.28-7.58 (m, 7H), 7.76-7.90 (m, 1H), 8.02 (d, J=8.2 Hz, 1H). 
       Intermediate 24 
     Methyl (3R)-7-amino-6-[(1-naphthyl)methyl]-4-oxo-1-thia-3a-aza-3-indancarboxylate 
       [0424]    
       
                 
         
             
             
         
       
     
         [0425]      1 H NMR (400 MHz, Methanol-d 4 ) δ 7.96-7.93 (m, 1H), 7.91 (d, J=8.3 Hz, 1H), 7.87-7.77 (m, 1H), 7.61-7.46 (m, 3H), 7.42 (d, J=7.0 Hz, 1H), 5.78 (dd, J=8.7, 1.7 Hz, 1H), 5.62 (s, 1H), 4.43 (s, 2H), 4.08 (dd, J=12.1, 8.7 Hz, 1H), 3.90 (dd, J=12.1, 1.7 Hz, 1H), 3.79 (s, 3H). 
       Intermediate 25 
     Methyl 7-cyclopropyl-4-oxo-6-[(2,3-xylyloxy)methyl]-1,3a-diaza-3-indancarboxylate 
       [0426]    
       
                 
         
             
             
         
       
     
         [0427]      1 H NMR (400 MHz, CDCl 3 ) δ=0.62-0.71 (m, 2H), 0.86-0.99 (m, 2H), 1.58-1.67 (m, 1H), 2.23 (s, 3H), 2.29 (s, 3H), 3.52 (dd, J=2.3, 11.7 Hz, 1H), 3.67 (dd, J=8.6, 11.7 Hz, 1H), 3.80 (s, 3H), 5.02-5.11 (m, 2H), 5.61 (dd, J=2.3, 8.5 Hz), 6.58-6.59 (m, 1H), 6.68-6.71 (m, 1H), 6.79-6.83 (m, 1H), 7.02-7.07 (m, 1H). 
       Intermediate 26 
     Methyl (4R)-2-(methoxymethyl)Δ 2 -1,3-thiazoline-4-carboxylate 
       [0428]    
       
                 
         
             
             
         
       
     
         [0429]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.43 (s, 3H), 3.55 (dABq, J=9.3, 15.5 Hz, 2H), 3.81 (s, 3H), 4.28-4.37 (m, 2H), 5.13 (tt, J=9.3, Hz, 1H). 
       Intermediate 27 
     Methyl (4R)-2-(2,2,2-trifluoroethyl)Δ 2 -1,3-thiazoline-4-carboxylate 
       [0430]    
       
                 
         
             
             
         
       
     
         [0431]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.32-3.50 (m, 2H), 3.59-3.75 (m, 2H), 3.83 (s, 3H), 5.10-5.18 (m, 1H). 
       Intermediate 28 
     Methyl (4R)-2-[(methylsulfonyl)methyl]Δ 2 -1,3-thiazoline-4-carboxylate 
       [0432]    
       
                 
         
             
             
         
       
     
         [0433]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 3.10 (s, 3H), 3.65-3.76 (m, 2H), 3.82 (s, 3H), 4.2 (s, 2H), 5.23 (t, J=9.0 Hz, 1H). 
       Intermediate 29 
     Methyl (4R)-2-[(benzyloxycarbonylamino)methyl]Δ 2 -1,3-thiazoline-4-carboxylate 
       [0434]    
       
                 
         
             
             
         
       
     
         [0435]      1 H NMR (400 MHz, Chloroform-d) δ 7.41-7.28 (m, 5H), 5.48 (s, 1H), 5.14 (s, 2H), 5.14-5.04 (m, 1H), 4.25 (d, J=5.6 Hz, 2H), 3.81 (s, 3H), 3.70-3.41 (m, 2H). 
       Intermediate 30 
     5-[1-Hydroxy-2-(2,3-xylyl)ethylidene]-2,2-dimethyl-1,3-dioxane-4,6-dione 
       [0436]    
       
                 
         
             
             
         
       
     
         [0437]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 1.76 (s, 6H), 2.20 (s, 3H), 2.31 (s, 3H), 4.53 (s, 2H), 7.02-7.14 (m, 3H). 
       Intermediate 31 
     5-(2-Chloro-1-hydroxyethylidene)-2,2-dimethyl-1,3-dioxane-4,6-dione 
       [0438]    
       
                 
         
             
             
         
       
     
         [0439]      1 H-NMR, 400 MHz, (CDCl 3 ) δ 1.76 (s, 6H), 4.88 (s, 2H). 
       Intermediate 32 
     5-[1-Hydroxy-2-(1-naphthyl)ethylidene]-2,2-dimethyl-1,3-dioxane-4,6-dione 
       [0440]    
       
                 
         
             
             
         
       
     
         [0441]    Intermediate 40 was prepared in accordance with Yamamoto, Y.; Watanabe, Y.; Ohnishi, S.  Chem. Pharm. Bull.  1987, 35, 1860-1870. 
       Materials and Methods 
       [0442]    Cell Culture,  C. trachomatis  Infections, and Determination of  C. trachomatis  Infectivity. 
         [0443]    HeLa cells were infected with  C. trachomatis  serovar LGV-L2 at an MOI of 0.3. At 1 hpi, RPMI media containing the different dilutions of the tested compounds in DMSO were added. The different compounds were dissolved to 20 mM in DMSO and were then diluted with RPMI media to be added at 2.5 to 100 μM. After 44-48 h incubation, the cells were osmotically lysed by the addition of cold sterile distilled water to release infectious EB progeny. 5×SPG was added to equalize the osmotic pressure to a 1×SPG isotonic condition. An equal amount of HBSS (Hanks balanced salt solution) (Gibco/Invitrogen) was added to the lysate (yielding a 1:1 dilution) and 10-fold serial dilutions of the resulting mixture was used to infect fresh HeLa cells. At 1 hpi, the inoculum was replaced with RPMI media and the infection allowed to progress for 44-48 hours before fixation and staining. Fixation was performed by adding methanol for 5 minutes and the cells were subsequently washed with phosphate buffered saline (PBS). The chlamydial inclusions were stained by a primary rabbit anti- Chlamydia  antibody (generated in-house) and a secondary donkey anti-rabbit FITC-labelled antibody (Jackson ImmunoResearch). The DNA of the cells and  Chlamydia  was stained by the addition of DAPI. The stained cells were analyzed by an ArrayScan automated scanner (ArrayScan VTI HCS, Thermo Scientific). Data were presented as the relative numbers of IFUs in treated infections compared to the numbers of IFUs in DMSO-treated control infections. 
       Biological Data 
       [0444]    The compounds of Examples 1-33 were tested for inhibition of infectious  Chlamydia  progenies at concentrations of 2.5 μM, 10 μM, 50 μM and 100 μM. In this document μM stands for micromolar. The compounds of the present disclosure were considered to have antibacterial activity if they provided 50% or more inhibition of infectious  Chlamydia  progenies using at least one of the tested concentrations in Table 1. 
         [0000]    
       
         
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                   
                 % Inhibition of infectious Chlamydia 
                   
               
               
                 Example 
                 progenies 
               
             
          
           
               
                 number 
                 100 uM 
                 50 uM 
                 10 uM 
                 2.5 uM 
               
               
                   
               
             
          
           
               
                 1 
                   
                   
                   
                 100 
               
               
                 2 
                   
                   
                 100 
                 96 
               
               
                 3 
                   
                   
                 100 
                 95 
               
               
                 4 
                   
                   
                 100 
                 99 
               
               
                 5 
                   
                   
                 100 
                 100 
               
               
                 6 
                   
                   
                 100 
                 63 
               
               
                 7 
                   
                   
                 100 
                 90 
               
               
                 8 
                   
                   
                 100 
                 87 
               
               
                 9 
                   
                   
                 100 
                 89 
               
               
                 10 
                   
                   
                 98 
                 88 
               
               
                 11 
                   
                   
                 100 
                 97 
               
               
                 12 
                   
                   
                 100 
                 98 
               
               
                 13 
                   
                   
                 100 
                 75 
               
               
                 14 
                   
                   
                 100 
                 76 
               
               
                 15 
                   
                   
                 100 
                 84 
               
               
                 16 
                   
                   
                 100 
                 81 
               
               
                 17 
                   
                   
                 100 
                 79 
               
               
                 18 
                   
                   
                 12 
                 46 
               
               
                 19 
                   
                   
                   
                 100 
               
               
                 20 
                   
                   
                   
                 99 
               
               
                 21 
                   
                   
                   
                 99 
               
               
                 22 
                   
                   
                 100 
                 98 
               
               
                 23 
                   
                 98 
                 98 
               
               
                 24 
                   
                 99 
                 98 
               
               
                 25 
                   
                 97 
                 97 
               
               
                 26 
                   
                 87 
                 54 
               
               
                 27 
                   
                 98 
                 96 
               
               
                 28 
                 56 
               
               
                 29 
                 56 
               
               
                 30 
                 90 
                 67 
               
               
                 31 
                 86 
                 29 
               
               
                 32 
                 99 
                 91 
               
               
                 33 
                 50