Abstract:
Methods are described for assembly of DNA aptamer-magnetic bead (“MB”) conjugate plus aptamer-quantum dot (“QD”) aptamer-fluorescent nanoparticle or other aptamer-fluorophore, aptamer-chemiluminescent reporter, aptamer-radioisotope or other aptamer-reporter conjugate sandwich assays that enable adherence to glass, polystyrene and other plastics. Adherence to glass or plastics enables detection of surface-concentrated partitioning of fluorescence versus background (bulk solution) fluorescence in one step (without a wash step) even when the external magnetic field for concentrating the assay is removed. This assay format enables rapid, one-step (homogeneous) assays for a variety of analytes without wash steps that do not sacrifice sensitivity.

Description:
[0001]    This application is based upon and claims priority from U.S. Provisional application Ser. Nos. 61/066,506 and 61/132,147, which are incorporated herein by reference. 
     
    
     BACKGROUND OF THE INVENTION 
       [0002]    1. Field of the Invention 
         [0003]    The present invention relates to the field of aptamer- and nucleic acid-based diagnostics. More particularly, it relates to methods for the production and use of self-assembling DNA aptamer-magnetic bead (“MB”) conjugate combined with aptamer-quantum dot (“QD”) or other aptamer-fluorophore conjugate sandwich assays that naturally adhere to glass and certain plastics such as polystyrene (or derivatives thereof) to enable one-step (homogeneous) tests without a wash step even after an external magnetic field is removed. Conjugation of aptamers to the MBs or QDs and other fluorophores may be accomplished by simple chemical coupling reactions through bifunctional linkers, or key functional groups such as aldehydes, carbodiimides, carboxyls, N-hydroxy-succinimide (“NHS”) esters, N-oxy-succinimide (“NOS”) esters, thiols, etc. or via biotin-avidin, histidine-Nickel, or other high affinity linkage systems. This one-step, washless assay format has numerous applications for sensitive detection of foodborne pathogens on and in meats, poultry, serous fluids, dairy products, fruits, vegetables, and other food matrices. The assay is also applicable to environmental analyses in soil or muddy water samples and clinical and veterinary diagnostics performed directly on whole blood, urine, saliva or other body fluids with or without sample dilution, but without a wash step. The typical wash step involves purification by removal of unwanted materials contributing to background fluorescence. 
         [0004]    2. Background Information 
         [0005]    The most desirable of all diagnostic assay strategies are rapid one-step “bind and detect” or “homogeneous” assays that do not require a wash step and yet do not sacrifice a significant degree of sensitivity. Examples of successful one-step assay strategies include fluorescence polarization (“FP”) and fluorescence resonance energy transfer (“FRET”)-based assays. While both of these formats are popular, they tend to sacrifice sensitivity for speed in obtaining test results. Therefore, FP and FRET assays are typically relegated to clinical diagnostics for certain analytes that exist in relatively high concentrations (micro to milliMolar ranges) in blood, urine, or other body fluids. For analytes that exist in much lower concentrations, multi-step assays such as enzyme-linked immunosorbent assays (“ELISA”), radioimmunoassays (“RIA”) and other sandwich-formatted assays such as immunomagnetic-electrochemiluminescence (“IM-ECL”) assays are required to detect nanogram, nanoMolar or lower amounts of various target analytes. Typically, these types of sandwich assays will require one or more wash steps, thereby slowing their execution speed. Wash steps will be known to one skilled in the art as a generally necessary step to remove unwanted materials (besides the detected target analyte) to prevent high background fluorescence signals. Eliminating the need for a wash step is found to be desirable in the present invention because it can enhance the speed and accuracy of many assays. 
         [0006]    DNA is well known to adhere to some glass surfaces especially if the surface is charged by rubbing. This principle is used in the electrostatic collection of genomic DNA from cell lysates which is known as “spooling” of DNA with a charged glass rod. Similarly, Allemand et al., Bensimon et al., Buck and Andrews, Dudley et al., Klein et al., Labit et al., Michalet et al., Moscoso et al., and Torres et al. teach adherence of DNA, bacteria, biofilms, and other materials to polystyrene by electrostatic and hydrophobic or other weak forces. However, Allemand et al., Bensimon et al., and Klein et al. emphasize that DNA is far more likely to bind to polystyrene and other plastics at its free 3′ or 5′ ends than in the mid-regions and that such binding is not instantaneous (requires one or more minutes of residence time for DNA to bind to plastic) and is pH-dependent with optimal pH for binding being acidic (at an approximate pH of 5.5, which is below the range of most biological assays). Bensimon et al. (1994) have even suggested that DNA may couple covalently to polystyrene by electrophilic addition of 5′ or 3′ phosphate ends (in their phosphoric acid forms) to the pi double bonds of the styrene rings or free unpolymerized alkene ends of polystyrene fibers. Such covalent bonding of DNA aptamers to polystyrene would explain the very stable and long-lasting adherence of assay materials observed and reported herein and by Bruno et al. (2008) for their  Campylobacter  assay. 
         [0007]    While some species of bacteria can bind to plastics and glass, not all species can form such adherent biofilms. In the presently described assays, attachment of the assay components (DNA aptamers, MBs and QDs) have occurred in the presence and absence of target bacteria. Conversely, immunomagnetic (“antibody-MB”) sandwich assays do not adhere to polystyrene very well at neutral or acidic pH, presumably because protein antibodies do not adhere well to plastic or glass materials at neutral or acidic pH. Proteins such as antibodies are well known to adhere to polystyrene microtiter plate wells at alkaline pH values as in the popular ELISA test formats. However, the pH for adherence of antibodies and proteins in ELISA assays is typically 8.0-9.5 and clearly not acidic as in the presently described DNA-adherent assays. Therefore, the DNA aptamer is considered to be the key component which enables adherence to polystyrene or glass or derivatives thereof and thus enables one-step washless assays. While some species of bacteria may contribute to overall adherence to the inner face of a cuvette, the DNA aptamer component appears sufficient to enable adherence of the aforementioned assays in the magnetized region because assay components (aptamer-MB conjugates) will adhere to plastic and glass even in the absence of captured bacterial cells. 
       RELATED LITERATURE 
       [0000]    
       
         Allemand J. F., et al. pH-dependent specific binding and combing of DNA. Biophys. J. 73:2064-2070, 1997. 
         Bensimon A., et al. Alignment and sensitive detection of DNA by a moving interface. Science. 265:2096-2098, 1994. 
         Bensimon D., et al. Stretching DNA with a receding meniscus: experiments and models. Phys. Rev. Lett. 74:4754-4757, 1995. 
         Bruno J. G., Phillips T., Carrillo M. P., Crowell R. Plastic-adherent DNA aptamer-magnetic bead and quantum dot sandwich assay for  Campylobacter  detection. J. Fluorescence. In Press, 2008. 
         Bruno J. G., Carrillo M. P., Phillips T., Crowell R. Initial development of competitive FRET-aptamer assays for monitoring bone metabolism. J. Clin. Ligand Assay. In Press, 2008. 
         Bruno J. G., Carrillo M. P., Crowell R. Preliminary development of DNA aptamer-Fc conjugate opsonins. J. Biomedical Materials Research-Part A, In Press, 2008. 
         Bruno J. G., Carrillo M. P., Phillips T. In vitro antibacterial effects of anti-lipopolysaccharide DNA aptamer-C1qrs complexes. Folia Microbiologica. 53:295-302, 2008. 
         Bruno J. G., Carrillo M. P., Phillips T., King B. Development of DNA aptamers for cytochemical detection of acetylcholine. In Vitro Cell. Develop. Biol.—Animal. 44:63-72, 2008. 
         Bruno J. G., Carrillo M. P., Phillips T. Development of DNA aptamers to a Foot-and-Mouth Disease peptide for competitive FRET-based detection. J. Biomolecular Techniques. 19:109-115, 2008. 
         Bruno J. G., Carrillo M. P., Phillips T. Effects of immobilization chemistry on enzyme-linked aptamer assays for  Leishmania  surface antigens. J. Clinical Ligand Assay. 30:37-43, 2007. 
         Bruno J. G., Francis K., Ikanovic, M., et al. Reovirus detection using immunomagnetic-fluorescent nanoparticle sandwich assays. J. Bionanoscience. 1:84-89, 2007. 
         Buck J. W. and Andrews J. H. Localized, positive charge mediates adhesion of Rhodosporidium torulides to barley leaves and polystyrene. Appl. Environ. Microbiol. 65:2179-2183, 1999. 
         Dudley E. G., et al. An Inch plasmid contributes to the adherence of the atypical enteroaggregative  Escherichia coli  strain C1096 to cultured cells and abiotic surfaces. Infect. Immun. 74:2102-2114, 2006. 
         Dwarakanath S., Satyanarayana S., Bruno J. G., et al. Ultra sensitive fluorescent nanoparticle-based binding assays for foodborne and waterborne pathogens of clinical interest. J. Clinical Ligand Assay. 29:136-142, 2006. 
         Ikanovic M., Rudzinski W. E., Bruno J. G., Dwarakanath S., et al. Fluorescence assay based on aptamer-quantum dot binding to  Bacillus thuringiensis  spores. J. Fluorescence. 17:193-199, 2007. 
         shi S. et al. Selection, characterization, and application of DNA aptamers for the capture and detection of  Salmonella enterica  serovars. Molec. Cell Probes. In Press, 2008. 
         Klein D. C. G., et al. Ordered stretching of single molecules of deoxyribose nucleic acid between microfabricated polystyrene lines. Appl. Phys. Lett. 78:2396-2398, 2001. 
         Labit H., et al. A simple and optimized method of producing silanized surfaces for FISH and replication mapping on combed DNA fibers. BioTechniques. 45:649-658, 2008. 
         Michalet X., et al. Dynamic molecular combing: stretching the whole human genome for high-resolution studies. Science. 277:1518-1523, 1997. 
         Moscoso M. et al. Biofilm formation by  Streptococcus pneumoniae : Role of choline extracellular DNA, and capsular polysaccharide in microbial accretion. J. Bacteriol. 188:7785-7795, 2006. 
         Quast B. A compact, handheld laboratory fluorometer. American Biotechnol Lab 18:68, 2001. 
         Torres A. G., et al. Differential binding of  Escherichia coli  O157:H7 to alfalfa, human epithelial cells, and plastic is mediated by a variety of surface structures. Appl. Environ. Microbiol. 71:8008-8015, 2005. 
       
     
       SUMMARY OF THE INVENTION 
       [0030]    Herein is described a new type of aptamer-MB-aptamer-QD sandwich assay and its derivative formats with variations in the fluorophore component that can be accomplished in one-step, obviating a wash step, by collecting the MBs with a strong external magnetic field onto a glass, polystyrene, other plastic or coated surface such as the inner face of a cuvette. Collection of the MBs and all attached assay components, including DNA aptamers, MBs, fluorophores and the captured analytes, into a small area on the plastic surface thereby focuses fluorescence intensity of the assay due to capture of the analyte in a thin planar area of adherence. Thus, when the adherent material is illuminated even in nearly opaque matrices such as foods or blood, the fluorescence can be detected with ultra sensitivity over background autofluorescence from the bulk solution due to partitioning and concentrating of the assay materials and captured analytes to the area of adherence. Fluorescence from uncaptured aptamer-QD or aptamer-fluorophore conjugates in the bulk solution contributes to background fluorescence, but its contribution to the total fluorescence signal is greatly minimized because it is not concentrated to the area of assay adherence. Any aptamer-QD or aptamer-fluorophore conjugates that do not bind the analyte and aptamer-MB conjugates will not be pulled toward the plastic surface nor adhere to the surface significantly and will not contribute significantly to the detection signal, but will contribute to the much weaker background fluorescence “noise” in the bulk solution. The combination of high aptamer affinity, the MBs ability to be concentrated in a defined area, and the long Stoke&#39;s shift of red-emitting QDs (i.e., high energy ultraviolet excitation with emission in the red region of the spectrum above 600 nm) contribute to the ultra sensitive nature of this one-step washless assay format. However, adherence of the assay materials and captured analytes to a small area on a clear plastic or glass surface even when the external magnetic field is removed is the key factor that enables one-step washless detection. 
         [0031]    The present invention provides for the assembly of DNA and RNA aptamer-MB conjugates for capture of target analytes with aptamer-QD or other aptamer-fluorophore conjugates. The target analytes are molecules that it is desirable to detect such as, pathogenic bacteria, viruses, parasites, leukocytes, cancer cells, proteins, other macromolecules, toxins, pollutants, drugs, explosives, proteins, viral capsid proteins, viral polymerases, biotoxins such as bacterial toxin, botulinum, cholera, tetanus, staphylococcal enterotoxin, shigatoxins or verotoxins, algal toxins, such as brevetoxin, ciguatoxin, cyanotoxin, or saxitoxin, snake or spider venoms, clinically relevant proteins or portions of proteins (peptides) such as bone marker (e.g., collagen breakdown peptides such as CTx, NTx, OCF, Cathepsin K or its precursor ProCathepsin K, deoxypyridinoline, pyridinoline, lysyl pyridinoline, or hydroxylysyl pyridinoline) cytokines and interleukins, markers of myocardial infarctions (troponin, myoglobin, etc.), kidney disease, antibodies, autoimmune disorders, arthritis, or other clinically relevant macromolecules such as lipopolysaccharides (LPS, endotoxins), and other small molecules (where “small molecules” are defined as being those that are less than 1,000 Daltons) such as with at least two distinct epitopes from a group including the following: pesticides, natural and synthetic amino acids and their derivatives, hydroxylysine, hydroxyproline, histidine, histamine, homocysteine, DOPA, melatonin, nitrotyrosine, short chain proteolysis products, cadaverine, putrescine, polyamines, spermine, spermidine, deoxypyridinoline, pyridinoline, lysyl pyridinoline, or hydroxylysyl pyridinoline, nitrogen bases of DNA or RNA, nucleosides, nucleotides, nucleotide cyclical isoforms, cAMP, cGMP, cellular metabolites, urea, uric acid, pharmaceuticals, therapeutic drugs, vitamins, illegal drugs, narcotics, hallucinogens, gamma-hydroxybutyrate (GHB), cellular mediators, cytokines, chemokines, immune modulators, neural modulators, neurotransmitters such as acetylcholine, inflammatory modulators, prostaglandins, prostaglandin metabolites, nitoaromatic and nitramine explosives, explosive breakdown products (e.g., DNT) or byproducts, quorum sensing molecules such as AHLs, steroids, hormones, and their derivatives. 
         [0032]    A fluorophore is a fluorescent component, or functional group, bound to a molecule. A fluorophore can be a dye, a glowing bead, a glowing liposome, a quantum dot (“QD”), a fluorescent or phosphorescent nanoparticle (“NP”), a fluorescent latex particle or microbead, a fluorescent dye molecule, such as fluorescein, carboxyfluorescein and other fluorescein derivatives, rhodamine, and their derivatives, a fluorescence resonance energy transfer (“FRET”) complex such as an intrachain or competitive FRET-aptamer, or any other glowing entity capable of forming a covalent bond with the aptamer. As used herein, “other aptamer-fluorophore conjugates” includes those aptamers having a fluorophore bonded to them, such as, in addition to those listed otherwise herein, aptamer-fluorescent dye conjugates, aptamer-fluorescent microbead conjugates, or aptamer-liposome conjugates containing fluorescent dyes. In the present invention, the fluorophore acts to “report” detection of the target analytes in one rapid and washless step. The only requirement of the target is that it contains two accessible epitopes of the same or different composition and conformation to enable a sandwich assay with capture and reporter aptamer components. 
         [0033]    The present invention utilizes a one-step assay format, which can be used for sandwich assay to detect and quantify said target analyte in said bulk solution, as well as fluorescence intensity, time-resolved fluorescence, chemiluminescence, electrical detection, electrochemical detection, electrochemiluminescence, phosphorescence, or radioisotopic detection. The one-step nature of the assay stems from the fact that the assay components capture the analyte and then stick or adhere to the inner surface of the assay substrate, generally expected to be a polystyrene plastic, glass, or other type of cuvette that is transparent or translucent enough so as to allow fluorescent light propagation, in a highly magnetized region for a brief time (5-10 minutes). 
         [0034]    More specifically, the one-step nature of the assay stems from the ability, after the application of an external magnetic field, to magnetically separate or partition the assay materials (aptamer-MBs and aptamer-QDs or other aptamer-fluorophore conjugates) from the bulk solution and allow these materials to bind or adhere to a surface such as the inner face of a polystyrene or glass cuvette via the attractive or covalent forces between DNA and some plastics or glass, thereby increasing the signal-to-noise ratio at the surface where the magnet was placed even after the magnet or magnetic field is removed to enable fluorescence detection. The sticking of brightly fluorescing analytes to the inner plane of the cuvette leads to the ability to discriminate the sample&#39;s more intense fluorescence from background or target fluorescence from bulk solution or “signal from noise” and to make one-step homogeneous assays possible. Adherence of assay materials to the cuvette constitutes a technique that even allows for detection in dense food samples (e.g. milk, chicken and beef juice, and egg yolk samples). 
         [0035]    A typical one-step aptamer-magnetic bead plus aptamer-quantum dot cuvette assay or test will consist of the following two components synthesized and added in any order: 1) One-hundred μg of 5′-amino modified aptamer DNA specific for one epitope on the target analyte plus 10 mM BS 3  [Bis(sulfosuccinimidyl) suberate] or other appropriate amine-reactive bifunctional linker such as EDC [1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride], Sulfo-EGS [Ethylene glycol bis(sulfosuccinimidylsuccinate)], Sulfo-SMCC [Sulfosuccinimidyl 4-[N-maleimidomethyl]-cyclohexane-1-carboxylate], glutaraldehyde, etc. plus 8 μM Qdot 655 ITK reagent (Invitrogen Corp.). These components are mixed in a 1 ml volume of 1× binding buffer (“1XBB”; 0.5 M NaCl, 10 mM Tris-HCl, 1 mM MgCl 2 , pH 7.5-7.6) for 30 mM at room temperature (“RT”). This aptamer-QD component is purified through Sephadex G-25 or another suitable size exclusion chromatography matrix. 2) One-hundred μg of a second 5′-amine-modified DNA aptamer with specificity to a second epitope on the target analyte plus 10 μl of tosyl-activated MBs (approximately 1×10 6  MBs, 1 to 5 microns in diameter). This aptamer-MB component is incubated at 37° C. for 2 or more hours and then collected with a strong magnet and washed 3 times in 1XBB. These two major components (aptamer-QD and aptamer-MB conjugates) are added to a polystyrene or other plastic cuvette with the addition of 1XBB up to a total volume of 2 ml. The cuvette is then lyophilized, back flushed with nitrogen gas and capped for long-term storage. 
         [0036]    The invention has been described above in a typical embodiment and amounts of the assay components for food safety testing for low numbers of pathogenic bacteria. However, broad ranges of detection are required for other types of analytes. Therefore, considering aptamer affinity ranges and ranges of detectable fluorescence, the one-step cuvette assays may be described based on the following ratios of ranges for the two major assay components:
       1) Aptamer-QD reagents: 0.5-50 nanoMoles of 5′-amono-DNA aptamer (or 10-1,000 μg of 60-100 base DNA in general) plus 10-20 miliMoles of bifunctional linker (BS 3  etc., linkers are in excess) plus 0.8-80 μMoles of QDs.   2) Aptamer-MB reagents: 0.5-50 nanoMoles of 5′-amino-DNA per 10 5 -10 7  tosyl-MBs or other appropriately derived MBs for DNA conjugation.       
 
         [0039]    In general, affinities for antibodies and aptamers, 10-fold ranges for each assay component (i.e., 10-fold lower and higher) are anticipated by the current invention. The amounts of the assay components are intended to be varied, because the present invention envisions assays of varying sensitivity. Thus, the same basic assay can have assay component amounts modified to allow for situations wherein extreme sensitivity is required, and others situations wherein less sensitivity is acceptable for the application. 
         [0040]    Prior to use, the one-step cuvette assay is reconstituted with a bulk solution which is to be tested for the presence of the desired target analyte. The bulk solution, which is in an amount anticipated to be approximately 2 ml, can be any number of various sample fluid matrices possibly containing target analytes including, but not limited to: natural waters, buffer, or diluted or undiluted food samples (e.g., milk, yoghurt, cheeses prior to solidification, meat juices, fruit juices, eggs, rinse waters from fruit and vegetable surfaces, diluted peanut butter, etc.), diluted whole blood, serum, urine, sputum or other body fluid samples. 
         [0041]    Along with the bulk solution, an aptamer-magnetic bead conjugate (“aptamer-MB”), and an aptamer-fluorophore conjugate are added, or can be lyophilized together in situ (in a cuvette) prior to adding the target analyte. The aptamer conjugates are chosen based upon the aptamer-MB being able to bind with the target analyte at a first binding site on the target analyte, and the aptamer-fluorophore conjugate being able to bind with the target analyte at a second binding site on the target analyte. Thus, if the target analyte is present in the bulk solution, both the aptamer-MB and an aptamer-fluorophore conjugates bind with the target analyte to form an analyte-aptamer-fluorophore complex. It is also necessary that the aptamer-MBs will not bind, base pair, or hybridize with the aptamer-fluorophores in the bulk solution. If they were to attach to each other in some way, in competition with the target analyte, then the assay would produce false positives because the MB would pull the aptamer-MB-fluorophore (without a target analyte) over to the cuvette translucent surface area to be assayed. 
         [0042]    The cuvette is recapped, shaken and mixed periodically over a 15-20 minute period, allowing the aptamer-MBs to bind with target analytes at the first binding site and the aptamer-fluorophore conjugates to bind with the target analyte at the second binding site to form an analyte-aptamer-fluorophore complex. Then the cuvette is added to a rack or other device with an external magnet set at the appropriate height to cause the analyte-aptamer-fluorophore complexes to adhere to the cuvette translucent surface area by applying an external magnetic field to attract the magnetic beads. Attracted by the magnetic field, the magnetic bead pulls the remainder of the analyte-aptamer-fluorophore complex which collects any captured analytes in a band (rectangular or square) or circular pattern at the level of a fluorometer&#39;s light path. The MBs with captured assay and target analytes are collected for 5 or more minutes and then the external magnet is removed, leaving adherent fluorescent MBs, assay and target analyte components adhering on the inner surface of the plastic cuvette as shown in  FIG. 1 . It is this partitioning and concentrating of the assay components and captured analytes to a thin adherent film on the inner face of the cuvette which enables discrimination of the intense assay fluorescence from the much weaker fluorescence of the bulk solution behind the adherent material. Thus, the analyte-aptamer-fluorophore complexes are effectively partitioned away from the remaining bulk solution to enhance detectability. This partitioning provides for a one-step, homogenous assay with high signal-to-noise ratio when the adherent assay is placed in a fluorometer and quantified with the appropriate excitation and emission wavelengths. 
         [0043]    Although described above as a cuvette, the present invention is effective in any number of container or vessel geometries. Thus, the method of the present invention may be run in a tube, vial, dish, flow cell, cassette, cartridge, microfluidic chip, and any other similar type of containers. And, the container can be composed of a plethora of materials, in any shape and of any type as long as a planar area of assay material attachment in a viewing “window” is provided and nucleic acid aptamers can adhere to the material. Therefore, the assay format may also be applied to a flattened plastic or glass cassette or cartridge in which assay components might be magnetically pulled along a channel or path by an external magnet. Upon reaching a clear plastic or glass detection window the assay components would be allowed to reside in the detection window where they could adhere to the window&#39;s surface and be concentrated away from the bulk solution by the external magnet. Hence, several embodiments or geometries for the assay vessel are envisioned so long as the cuvette has a translucent surface area so as to enable a fluorescent assay. For example, the cuvette translucent surface area, on which said analyte-aptamer-fluorophore complex adheres, may be formed as a square, rectangular, round, oval, or flat container, vial, tube, cylinder, cassette, or cartridge. 
         [0044]    It is anticipated that the cuvette may be made from polystyrene, clear plastic, or glass. But in addition, the chemistry of DNA attachment to the glass or plastic is not restricted to natural glass or simple polystyrene. Rather, logical derivative plastics and coatings (e.g., silanes, etc.) that include alkenes for electrophilic addition of DNA and hydrophobic coatings that may encourage weak force (van der Waals or dipole-dipole) interactions and adherence of DNA to the coated glass or plastic are also envisioned. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0045]      FIG. 1 . is a schematic illustration of how the one-step adherent sandwich assay forms and is drawn to the inner face of a plastic or glass cuvette by an external magnet. 
           [0046]      FIG. 2 . shows line graphs plotting relative fluorescence intensity against the concentration of  Campylobacter jejuni  ( C. jejuni ) bacteria. 
           [0047]      FIG. 3 . shows a series of fluorescence emission spectra related to detection of serial ten-fold dilutions of  Campylobacter jejuni  bacteria in neat buffer (1XBB) and various diluted food matrices as indicated in the figure. Excitation was at 380 nm with a photomultiplier tube setting of 900 Volts. 
           [0048]      FIG. 4  illustrates a typical one-step assay capable of detecting 10 live  C. jejuni  bacteria in undiluted chicken “juice” (serous fluid collected from chicken legs prior to cooking). Data points represent the means and standard deviations of five independent readings (N=5). 
         DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT 
         [0049]    Referring to the figures,  FIG. 1 . provides a schematic representation of the one-step adherent sandwich assay concept. In this concept, a DNA or possibly an RNA aptamer has been conjugated to a magnetic bead and used to capture a target analyte (bacterial cell in this example). Capture is achieved by specific aptamer binding to an epitope on the bacterial surface. Likewise, another epitope is bound by an aptamer-quantum dot conjugate or other aptamer-fluorophore reporter reagent simultaneously for fluorescent detection. Because the sandwich assay contains DNA or RNA, it is subject to adhering to some forms of charged glass or charged or uncharged plastics such as polystyrene and its derivatives by electrostatic and/or other weak forces such as dipole-dipole or Van der Waals interactions and possibly covalent electrophilic addition to alkenes or the styrene rings (Bensimon et al., 1994). Adherence is promoted by the addition of an external attractive magnetic force such as a strong Cobalt, Neodynium, or other rare earth magnet. After the external magnet is disengaged, the assay materials still adhere to the inner face of the cuvette due to interaction of DNA with the polystyrene or other plastic or glass materials. This adherence partitions the assay along with captured and labeled bacteria or other analytes from the bulk solution. If the solution is illuminated from the opposite side by an excitation source and the cuvette face with adherent assay materials is placed proximal to a photodetector, rapid, sensitive, one-step detection is enabled. Once adherence of all the aptamer-MB-bacteria-aptamer-QD complexes occurs on the surface, the adherent material emits a much brighter fluorescent signal than the bulk solution which contains free aptamer-QD or aptamer-fluorophore conjugates. 
           [0050]      FIG. 2 . shows line graphs plotting relative fluorescence intensity against the concentration of  Campylobacter jejuni  ( C. jejuni ) bacteria detected in neat buffer (1× binding buffer; 1XBB) down to a level of approximately 2 bacterial cells per milliliter using the one-step adherent DNA aptamer-MB-aptamer red QD (Q-dot 655 nm) sandwich assay without a wash step. Five independent readings were taken per data point with the green (Rhodamine) channel of a Turner Biosystems, Inc. handheld fluorometer. Error bars which are not visible due to their small numerical values represent the standard deviations of the 5 readings. The preferred embodiment for the adherent one-step washless aptamer-MB/aptamer-QD or aptamer-fluorophore assays is in a plastic polystyrene cuvette using lyophilized (freeze-dried) sandwich assay materials with long shelf-life that are rehydrated as needed. Their fluorescence can be assessed after a 15-20 minute capture and 5 minute magnetic collection period via a table top spectrofluorometer, or portable fluorometers such as the Turner Biosystem&#39;s Picofluor™ or Invitrogen&#39;s Q-Bit™ or other such fluorescence reader devices. The primarily linear photoresponse to logarithmic changes in bacterial concentration seen in  FIGS. 2 and 4  is probably attributable to a photodiode detector in the fluorometer, such as is found in the Picofluor™, versus the more sensitive and exponentially responsive photomultiplier tube (PMT) used for data collection by a spectrofluorometer in  FIG. 3 . 
           [0051]      FIG. 3 . shows a series of fluorescence emission spectra from ten-fold serial dilutions of 25 million heat-killed  C. jejuni  bacteria per ml (highest peak) to 2.5 bacteria per ml and then zero bacteria per ml (lowest peak) detected by use of a Cary-Varian spectrofluorometer and the one-step plastic-adherent aptamer-MB/aptamer-red QD (Q-dot 655 nm) sandwich assay without a wash step directly in various food matrices as indicated. The arrows indicate the direction of increasing 2-fold dilutions or decreasing bacterial concentration. The assays are generally described herein as using a fluorescence intensity reporter method, which is a simple measure of fluorescence brightness, for detecting and quantifying the analyte-aptamer conjugate. Alternatively, the fluorescence intensity reporter method may be substituted by time-resolved fluorescence, chemiluminescence, electrical detection, electrochemical detection, electrochemiluminescence, phosphorescence, or radioisotopic detection instead of simple fluorescence intensity-based detection. 
           [0052]      FIG. 4 . illustrates a typical one-step assay capable of detecting 10 live  C. jejuni  bacteria in chicken juice (collected blood and fat globules from a fresh grocery store chicken product). Five independent readings were taken per data point with the green (Rhodamine) channel of a Turner Biosystems, Inc. handheld Picofluor™ fluorometer. Error bars which are barely visible due to their small numerical values represent the standard deviations of the 5 readings. 
       
    
    
       [0053]    No wash steps are required and detection can be achieved directly in various food, environmental, or body fluid matrices as illustrated in  FIGS. 3 and 4 . 
       Example 1 
     One-Step, Washless Ultra sensitive Detection of  Campylobacter jejuni  in Various Food Matrices 
       [0054]    The invention has been used to detect as few as 2 live or dead  C. jejuni  bacterial cells (a common foodborne pathogen) in neat buffer and various food matrices as shown in  FIGS. 2-4 . In these assays, two different  C. jejuni  Sequences (designated C2 and C3 or SEQ ID NOs 2 and 3) were 5′-amine modified during solid-phase DNA synthesis and attached to either 1,000 tosyl-M280 (2.8 micron diameter) Dynal (Invitrogen, Inc.) MBs or 0.24 picoliters of Q-dot 655 ITK reagent (Invitrogen, Inc.) per test. The C2 aptamer (SEQ ID NO. 2) was used for capture on the surface of tosyl-MBs and the C3 aptamer (SEQ ID NO. 3) was used as the reporter reagent after attachment to the Q-dot 655 ITK reagent via BS 3  (bis-suberate bifunctional linker from Pierce Chemical Co.). The reagents were purified, mixed together and lyophilized in plastic cuvettes. The powdered assays were later backflushed with nitrogen and capped. Upon rehydration, the adherent one-step sandwich assays were used to detect live or dead (heat-killed)  C. jejuni  cells with the very sensitive results depicted in  FIGS. 2-4 . Other aptamers chosen for capture and reporter functions from SEQ ID NOs. 1-6 can be substituted in  Campylobacter  assays which are functional, but result in somewhat less sensitive detection. 
       Example 2 
     One-Step, Washless Ultrasensitive Detection of  Escherichia coli  O157:H7 and other toxigenic (Shiga or Verotoxin-producing)  E. coli  in Various Food Matrices 
       [0055]    The present invention has potential to be used for detection of enterohemorraghic  E. coli  O157:H7 in and on various foods via binding of aptamers to the outer saccharides of 0157 lipopolysaccharide (LPS) and the H7 flagellar antigen. Aptamer sequences from SEQ ID NOs. 7-20 could be chosen for capture (aptamer-MB conjugate) or reporter (aptamer-fluorophore conjugate) functions and used to detect  E. coli  O157:H7 in or on foods. Alternatively, outer membrane proteins (OMPs) common to many species of  E. coli  can be used for aptamer-MB-based capture (or identification) of the  E. coli  bacterial cells followed by specific identification of the  E. coli  strain or serotype using LPS-specific aptamer-QD reporter reagents to complete the sandwich assay. Aptamer SEQ ID NOs. 279-322 can be used for  E. coli  OMP recognition and capture. In yet another embodiment non-O157:H7 toxigenic  E. coli  bacteria can be sensitively identified by their secretion of Shiga or Verotoxins (types 1 and 2 or Stx-1 and Stx-2). Many other strains of  E. coli  including O126 can produce deadly disease in humans and the common thread among these lethal pathogens is the secretion of Stx. Therefore, a very useful embodiment of the invention would be detection of Stx-1 and/or Stx-2 using any of the DNA aptamer sequences identified by SEQ ID NOs. 323-352. 
       Example 3 
     One-Step, Washless Ultrasensitive Detection of  Listeria monocytogenes  in Various Food Matrices 
       [0056]    The present invention has potential to be used for detection of lethal  L. monocytogenes  in and on various foods via binding to the listerolysin (LO) surface protein. Aptamer sequences from SEQ ID NOs. 21-52 could be chosen for capture (aptamer-MB conjugate) or reporter (aptamer-fluorophore conjugate) functions and used to detect LO and  L. moncytogenes  in or on foods. 
       Example 4 
     One-Step, Washless Ultrasensitive Detection of  Salmonella typhimurium  in Various Food Matrices 
       [0057]    The present invention has potential to be used for detection of  S. typhimurium  and other  Salmonella  species ( S. typhi  etc.) in and on various foods.  S. typhimurium  has been renamed  Salmonella enterica  serovar Typhimurium, but many microbiologists and lay people still refer to the microbe as  S. typhimurium . Aptamer sequences from SEQ ID NOs. 53-68 could be chosen for capture (aptamer-MB conjugate) or reporter (aptamer-fluorophore conjugate) functions for detection of  Salmonella typhimurium  LPS bacteria in or on foods. In addition, aptamer SEQ ID NOs. 353-392 could be used for capture or identification of  S. typhimurium  OMPs. These  S. typhimurium  DNA aptamer sequences are unique and bear no resemblance to those recently reported by Joshi et al. (2008). 
       Example 5 
     Detection of Fecal Contamination of Water Supplies 
       [0058]    The present invention has the potential to detect all species of  Escherichia coli  bacteria in recreational, treated waste water, and drinking water supplies using aptamer DNA SEQ ID NOs. 69-122 directed against common core components of LPS for capture and reporter functions. The present invention has the potential to detect all species of  Enterococcus  bacteria (another common fecal indicator organism) in recreational, treated waste water, and drinking water supplies using aptamer DNA SEQ ID NOs. 123-130 directed against common teichoic acid moieties for capture and reporter functions. 
       Example 6 
     Detection of  Leishmania  Parasites in Skin Lesions or Visceral Fluids 
       [0059]    The present invention has the potential to detect  Leishmania donovani  or  L. tropica  parasites in skin lesions of body fluids and other samples using aptamer DNA Sequences chosen from SEQ ID NOs. 131-134 directed against surface proteins of common to both  Leishmania  species for capture and reporter functions. 
       Example 7 
     Detection of Encapsultaed Vegetative  Bacillus anthracis  Bacteria in Blood and Body Fluids 
       [0060]    The invention has the potential to detect encapsulated  B. anthracis  (anthrax) vegetative bacteria in blood and body fluids and other samples using aptamer DNA Sequences chosen from SEQ ID NOs. 135-138 directed against surface poly-D-glutamic acid (PDGA) capsular materials for capture and reporter functions. 
       Example 8 
     Detection of Small Molecules (&lt;1,000 Daltons) in Environmental and Clinical Samples 
       [0061]    The invention has the potential to detect small molecules of &lt;1,000 Daltons, if the target has two distinct and accessible epitopes for attachment of capture and reported aptamers to enable a sandwich assay format. Among such small molecule targets would be organophosphorus pesticides (such as diazinon and malathion) in environmental water, soil, or mud samples as well as blood and body fluids and other samples using aptamer DNA Sequences chosen from SEQ ID NOs. 139-154 directed against different ends of the pesticide molecule for capture and reporter functions. In addition, vitamins such as 25-hydroxyvitamin D 3  (calcidiol; SEQ ID NOs. 243-274), the neurotransmitter acetylcholine (ACh; SEQ ID Nos. 393-416) might be viable targets for this novel adherent assay format 
       Example 9 
     Detection of Foot-and-Mouth Disease (FMD) and Related Viruses 
       [0062]    The invention has the potential to detect FMD and related viruses in blood and body fluids and other samples using aptamer DNA Sequences chosen from SEQ ID NOs. 155-164 directed against a conserved 16-amino acid peptide from several 0 serotypes of FMD for capture and reporter functions. 
       Example 10 
     Detection of Bone Resorption or Synthesis Markers in Blood and Body Fluids 
       [0063]    The invention has the potential to detect markers of bone loss such as cathepsin K, C-terminal telopeptides (CTx) and N-terminal telopeptides (NTx) of collagen, hydroxylysine (HL), osteocalcin fragments (OCF), etc. due to the effects of low gravity during lengthy spaceflights or osteoporosis and aging in blood, urine and other body fluids and other samples using aptamer DNA Sequences chosen from SEQ ID NOs. 165-242 directed against unique epitopes on each type of bone marker. The invention also has the potential to detect and discriminate various isomers of vitamin D associated with bone formation chosen from SEQ ID NOs. 243-274 for capture and reporter functions. 
       Example 11 
     Detection of Botulinum Toxin A and Related Biotoxins 
       [0064]    The invention has the potential to detect Clostridum botulinum toxins which affect humans and animals (serotypes A-F) and related bacterial, harmful algal bloom (HAB, dinoflagellate), marine (shellfish-related), or plant toxins such as tetanus toxin, cholera and diphtheria toxins, shiga and verotoxins, staphylococcal enterotoxins, cyanotoxins, azaspiracids, brevetoxins, ciguatoxins, gonyautotoxins, domoic acid isomers, maitotoxins, palytoxins, yessotoxins, saxitoxins, ricin, gelonin, abrin, spider and snake venoms, etc. in blood and body fluids and other samples using aptamer DNA sequences in the adherent sandwich format. Aptamer sequences chosen from SEQ ID NOs. 275-278 in particular can be used to for detection of botulinum type A light chains or the holotoxin. 
       Example 12 
     Detection of Quorum Sensing Molecules in Bacterial Infections 
       [0065]    Many species of bacteria are now known to communicate chemically via secreted small molecules. Many Gram negative bacterial pathogens commonly use a family of small molecules called acylhomoserine lactones (AHLs) to communicate between bacterial cells to sense when a critical concentration of cells or “quorum” has been reached to enable effective infection of a host organism. AHLs control induction of pathogenesis and virulence factors such as expression of adherence proteins and toxins. Therefore, early sensing of AHLs could indicate an imminent Gram negative bacterial infection and prompt a physician to administer the appropriate antibiotics to prevent an infection or more severe sepsis. AHLs do commonly possess two different ends or potential epitopes and are therefore potential candidates for the one-step plastic-adherent DNA aptamer-MB-aptamer-QD or other aptamer-reporter sandwich assays described herein. Sequence ID Nos. 417-426 illustrate potential aptamer DNA sequences developed against and reactive with the family of Gram negative bacterial AHLs for diagnostics. 
         [0066]    Although the invention has been described with reference to specific embodiments, this description is not meant to be construed in a limited sense. Various modifications of the disclosed embodiments, as well as alternative embodiments of the inventions will become apparent to persons skilled in the art upon the reference to the description of the invention. It is, therefore, contemplated that the appended claims will cover such modifications that fall within the scope of the invention. Such alternative embodiments may include, but are not limited to changes in the reporter method including chemiluminescence, electrical detection, electrochemical detection, electrochemiluminescence, phosphorescence, and radioisotopic detection instead of fluorescence-based detection. 
         [0000]    
       
         
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 DNA Aptamer Sequence ID Nos. 
               
               
                   
               
             
             
               
                   Campylobacter jejuni  Surface Protein Aptamers 
               
               
                 SEQ ID NO. 1 
               
               
                 (C1) 
               
               
                 CATCCGTCACACCTGCTCTGGGGAGGGTGGCGCCCGTCTCGGTGGTGTTG 
               
               
                 GCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 2 
               
               
                 (C2) 
               
               
                 CATCCGTCACACCTGCTCTGGGATAGGGTCTCGTGCTAGATGTGGTGTTG 
               
               
                 GCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 3 
               
               
                 (C3) 
               
               
                 CATCCGTCACACCTGCTCTGGACCGGCGCTTATTCCTGCTTGTGGTGTTG 
               
               
                 GCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 4 
               
               
                 (C4) 
               
               
                 CATCCGTCACACCTGCYCTGGAGCTGATATTGGATGGTCCGGTGGTGTTG 
               
               
                 GCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 5 
               
               
                 (C5) 
               
               
                 CATCCGTCACACCTGCYCYGCCCAGAGCAGGTGTGACGGATGTGGTGTTG 
               
               
                 GCTCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 6 
               
               
                 (C6) 
               
               
                 CATCCGTCACACCTGCYCYGCCGGACCATCCAATATCAGCTGTGGTGTTG 
               
               
                 GCTCCCGTATCA 
               
               
                   
               
               
                   E. coli  O157 Lipopolysaccharide (LPS) Aptamers 
               
               
                 SEQ ID NO. 7 
               
               
                 (E-5F) 
               
               
                 ATCCGTCACACCTGCTCTGGTGGAATGGACTAAGCTAGCTAGCGTTTTAA 
               
               
                 AAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 8 
               
               
                 (E-11F) 
               
               
                 ATCCGTCACACCTGCTCTGTAAGGGGGGGGAATCGCTTTCGTCTTAAGAT 
               
               
                 GACATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO.9 
               
               
                 (E-12F) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 10 
               
               
                 (E-16F) 
               
               
                 ATCCGTCACACCTGCTCTATCCGTCACGCCTGCTCTATCCGTCACACCTG 
               
               
                 CTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 11 
               
               
                 (E-17F) 
               
               
                 ATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGACGATTTGTAC 
               
               
                 AAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 12 
               
               
                 (E-18F) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACGAT 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 13 
               
               
                 (E-19F) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACGAT 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 14 
               
               
                 (E-5R) 
               
               
                 ATACGGGAGCCAACACCACCTTTTAAAACGCTAGCTAGCTTAGTCCATTC 
               
               
                 CACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 15 
               
               
                 (E-11R) 
               
               
                 ATACGGGAGCCAACACCATGTCATCTTAAGACGAAAGCGATTCCCCCCCC 
               
               
                 TTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 16 
               
               
                 (E-12R) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCAGGT 
               
               
                 GTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 17 
               
               
                 (E-16R) 
               
               
                 ATACGGGAGCCAACACCAGAGCAGGTGTGACGGATAGAGCAGGCGTGACG 
               
               
                 GATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 18 
               
               
                 (E-17R) 
               
               
                 ATACGGGAGCCAACACCATCTTGTACAAATCGTCTTGATATCTGCACATT 
               
               
                 TGATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 19 
               
               
                 (E-18R) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCCAT 
               
               
                 CTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 20 
               
               
                 (E-19R) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCCAT 
               
               
                 CTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                   Listeriolysin  (A surface protein on  Listeria   
               
               
                 monocytogenes) Aptamers 
               
               
                 SEQ ID NO. 21 
               
               
                 (LO-10F) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 22 
               
               
                 (LO-11F) 
               
               
                 ATCCGTCACACCTGCTCTGGTGGAATGGACTAAGCTAGCTAGCGTTTTAA 
               
               
                 AAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 23 
               
               
                 (LO-13F) 
               
               
                 ATCCGTCACACCTGCTCTTAAAGTAGAGGCTGTTCTCCAGACGTCGCAGG 
               
               
                 AGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 24 
               
               
                 (LO-15F) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACGAT 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 25 
               
               
                 (LO-16F) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACGAT 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 26 
               
               
                 (LO-17F) 
               
               
                 ATACGGGAGCCAACACCA 
               
               
                 CAGCTGATATTGGATGGTCCGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 27 
               
               
                 (LO-19F) 
               
               
                 ATCCGTCACACCTGCTCTTGGGCAGGAGCGAGAGACTCTAATGGTAAGCA 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 28 
               
               
                 (LO-20F) 
               
               
                 ATCCGTCACACCTGCTCTCCAACAAGGCGACCGACCGCATGCAGATAGCC 
               
               
                 AGGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 29 
               
               
                 (LO-10R) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCAGGT 
               
               
                 GTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 30 
               
               
                 (LO-11R) 
               
               
                 ATACGGGAGCCAACACCACCTTTTAAAACGCTAGCTAGCTTAGTCCATTC 
               
               
                 CACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 31 
               
               
                 (LO-13R) 
               
               
                 ATACGGGAGCCAACACCATCCTCCTGCGACGTCTGGAGAACAGCCTCTAC 
               
               
                 TTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 32 
               
               
                 (LO-15R) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCCAT 
               
               
                 CTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 33 
               
               
                 (LO-16R) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCCAT 
               
               
                 CTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 34 
               
               
                 (LO-17R) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 35 
               
               
                 (LO-19R) 
               
               
                 ATACGGGAGCCAACACCATTCTTGCTTACCATTAGAGTCTCTCGCTCCTG 
               
               
                 CCCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 36 
               
               
                 (LO-20R) 
               
               
                 ATACGGGAGCCAACACCAACCTGGCTATCTGCATGCGGTCGGTCGCCTTG 
               
               
                 TTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                   Listeriolysin  (Alternate form of  Listeria  surface 
               
               
                 protein designated “Pest-Free”) Aptamers 
               
               
                 SEQ ID NO. 37 
               
               
                 (LP-3F) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACGAT 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 38 
               
               
                 (LP-11F) 
               
               
                 ATCCGTCACACCTGCTCTAACCAAAAGGGTAGGAGACCAAGCTAGCGATT 
               
               
                 TGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 39 
               
               
                 (LP-13F) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 40 
               
               
                 (LP-14F) 
               
               
                 ATCCGTCACACCTGCTCTGAAGCCTAACGGAGAAGATGGCCCTACTGCCG 
               
               
                 TAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 41 
               
               
                 (LP-15F) 
               
               
                 ATCCGTCACACCTGCTCTACTAAACAAGGGCAAACTGTAAACACAGTAGG 
               
               
                 GGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 42 
               
               
                 (LP-17F) 
               
               
                 ATCCGTCACACCTGCTCTGGTGTTGGCTCCCGTATAGCTTGGCTCCCGTA 
               
               
                 TGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 43 
               
               
                 (LP-18F) 
               
               
                 ATCCGTCACACCTGCTCTGTCGCGATGATGAGCAGCAGCGCAGGAGGGAG 
               
               
                 GGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 44 
               
               
                 (LP-20F) 
               
               
                 ATCCGTCACACCTGCTCTGATCAGGGAAGACGCCAACACTGGTGTTGGCT 
               
               
                 CCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 45 
               
               
                 (LP-3R) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCCAT 
               
               
                 CTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 46 
               
               
                 (LP-11R) 
               
               
                 ATACGGGAGCCAACACCATCCAAATCGCTAGCTTGGTCTCCTACCCTTTT 
               
               
                 GGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 47 
               
               
                 (LP-13R) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCAGGT 
               
               
                 GTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 48 
               
               
                 (LP-14R) 
               
               
                 ATACGGGAGCCAACACCACCTACGGCAGTAGGGCCATCTTCTCCGTTAGG 
               
               
                 CTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 49 
               
               
                 (LP-15R) 
               
               
                 ATACGGGAGCCAACACCACGCCCCTACTGTGTTTACAGTTTGCCCTTGTT 
               
               
                 TAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 50 
               
               
                 (LP-17R) 
               
               
                 ATACGGGAGCCAACACCATACGGGAGCCAAGCTATACGGGAGCCAACACC 
               
               
                 AGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 51 
               
               
                 (LP-18R) 
               
               
                 ATACGGGAGCCAACACCACCCCCTCCCTCCTGCGCTGCTGCTCATCATCG 
               
               
                 CGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 52 
               
               
                 (LP-20R) 
               
               
                 ATACGGGAGCCAACACCAGTGTTGGCGTCTTCCCTGATCAGAGCAGGTGT 
               
               
                 GACGGAT 
               
               
                   
               
               
                   Salmonella typhimurium  lipopolysaccharide(LPS) 
               
               
                 Aptamers 
               
               
                 SEQ ID NO. 53 
               
               
                 (St-7F) 
               
               
                 ATCCGTCACACCTGCTCTGTCCAAAGGCTACGCGTTAACGTGGTGTTGGC 
               
               
                 TCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 54 
               
               
                 (St-10F) 
               
               
                 ATCCGTCACACCTGCTCTGGAGCAATATGGTGGAGAAACGTGGTGTTGGC 
               
               
                 TCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 55 
               
               
                 (St-11F) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 56 
               
               
                 (St-15F) 
               
               
                 ATCCGTCACACCTGCTCTGAACAGGATAGGGATTAGCGAGTCAACTAAGC 
               
               
                 AGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 57 
               
               
                 (St-16F) 
               
               
                 ATCCGTCACACCTGCTCTGGCGGACAGGAAATAAGAATGAACGCAAAATT 
               
               
                 TATCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 58 
               
               
                 (St-18F) 
               
               
                 ATCCGTCACACCTGCTCTACGCAACGCGACAGGAACATTCATTATAGAAT 
               
               
                 GTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 59 
               
               
                 (St-19F) 
               
               
                 ATCCGTCACACCTGCTCTCGGCTGCAATGCGGGAGAGTAGGGGGGAACCA 
               
               
                 AACCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 60 
               
               
                 (St-20F) 
               
               
                 ATCCGTCACACCTGCTCTATGACTGGAACACGGGTATCGATGATTAGATG 
               
               
                 TCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 61 
               
               
                 (St-7R) 
               
               
                 ATACGGGAGCCAACACCACGTTAACGCGTAGCCTTTGGACAGAGCAGGTG 
               
               
                 TGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 62 
               
               
                 (St-10R) 
               
               
                 ATACGGGAGCCAACACCACGTTTCTCCACCATATTGCTCCAGAGCAGGTG 
               
               
                 TGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 63 
               
               
                 (St-11R) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCAGGT 
               
               
                 GTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 64 
               
               
                 (St-15R) 
               
               
                 ATACGGGAGCCAACACCATGCTGCTTAGTTGACTCGCTAATCCCTATCCT 
               
               
                 GTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 65 
               
               
                 (St-16R) 
               
               
                 ATACGGGAGCCAACACCAGATAAATTTTGCGTTCATTCTTATTTCCTGTC 
               
               
                 CGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 66 
               
               
                 (St-18R) 
               
               
                 ATACGGGAGCCAACACCAACACATTCTATAATGAATGTTCCTGTCGCGTT 
               
               
                 GCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 67 
               
               
                 (St-19R) 
               
               
                 ATACGGGAGCCAACACCAGGTTTGGTTCCCCCCTACTCTCCCGCATTGCA 
               
               
                 GCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 68 
               
               
                 (St-20R) 
               
               
                 ATACGGGAGCCAACACCAAGGACATCTAATCATCGATACCCGTGTTCCAG 
               
               
                 TCATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 Fecal Contamination Indicator (Core LPS Antigens) 
               
               
                 Aptamers 
               
               
                 SEQ ID NO. 69 
               
               
                 (Glucosamine(G)1F) 
               
               
                 ATCCGTCACACCTGCTCTAATTAGGATACGGGGCAACAGAACGAGAG 
               
               
                 GGGGGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 70 
               
               
                 (G2F) 
               
               
                 ATCCGTCACACCTGCTCTCGGACCAGGTCAGACAAGCACATCGGATAT 
               
               
                 CCGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 71 
               
               
                 (G5F) 
               
               
                 ATCCGTCACACCTGCTCTTGAGTCAAAGAGTTTAGGGAGGAGCTAACA 
               
               
                 TAACAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 72 
               
               
                 (G7F) 
               
               
                 ATCCGTCACACCTGCTCTAACAACAATGCATCAGCGGGCTGGGAACGC 
               
               
                 ATGCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 73 
               
               
                 (G8F) 
               
               
                 ATCCGTCACACCTGCTCTGAACAGGTTATAAGCAGGAGTGATAGTTTC 
               
               
                 AGGATCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 74 
               
               
                 (G9F) 
               
               
                 ATCCGTCACACCTGCTCTCGGCGGCTCGCAAACCGAGTGGTCAGCACC 
               
               
                 CGGGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 75 
               
               
                 (G10F) 
               
               
                 ATCCGTCACACCTGCTCTGCGCAAGACGTAATCCACAAGACCGTGAAA 
               
               
                 ACATAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 76 
               
               
                 (G1R) 
               
               
                 ATACGGGAGCCAACACCATTCCCCCCTCTCGTTCTGTTGCCCCGTATCC 
               
               
                 TAATTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 77 
               
               
                 (G2R) 
               
               
                 ATACGGGAGCCAACACCAGCCGGATATCCGATGTGCTTGTCTGACCTG 
               
               
                 GTCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 78 
               
               
                 (G5R) 
               
               
                 ATACGGGAGCCAACACCACTGTTATGTTAGCTCCTCCCTAAACTCTTTG 
               
               
                 ACTCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 79 
               
               
                 (G7R) 
               
               
                 ATACGGGAGCCAACACCACCGCATGCGTTCCCAGCCCGCTGATGCATT 
               
               
                 GTTGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 80 
               
               
                 (G8R) 
               
               
                 ATACGGGAGCCAACACCAGATCCTGAAACTATCACTCCTGCTTATAAC 
               
               
                 CTGTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 81 
               
               
                 (G9R) 
               
               
                 ATACGGGAGCCAACACCAACCCGGGTGCTGACCACTCGGTTTGCGAG 
               
               
                 CCGCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 82 
               
               
                 (G10R) 
               
               
                 ATACGGGAGCCAACACCACTATGTTTTCACGGTCTTGTGGATTACGTC 
               
               
                 TTGCGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 83 
               
               
                 (KDO (K) Antigen 2F) 
               
               
                 ATCCGTCACACCTGCTCTAGGCGTAGTGACTAAGTCGCGCGAAAATCA 
               
               
                 CAGCATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 84 
               
               
                 (K5F) 
               
               
                 ATCCGTCACACCTGCTCTCAGCGGCAGCTATACAGTGAGAACGGACTA 
               
               
                 GTGCGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 85 
               
               
                 (K7F) 
               
               
                 ATCCGTCACACCTGCTCTGGCAAATAATACTAGCGATGATGGATCTGG 
               
               
                 ATAGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 86 
               
               
                 (K8F) 
               
               
                 ATCCGTCACACCTGCTCTGGGGGTGCGACTTAGGGTAAGTGGGAAAGA 
               
               
                 CGATGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 87 
               
               
                 (K9F) 
               
               
                 ATCCGTCACACCTGCTCTCAAGAGGAGATGAACCAATCTTAGTCCGAC 
               
               
                 AGGCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 88 
               
               
                 (K10F) 
               
               
                 ATCCGTCACACCTGCTCTGGCCCGGAATTGTCATGACGTCACCTACAC 
               
               
                 CTCCTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 89 
               
               
                 (K2R) 
               
               
                 ATACGGGAGCCAACACCAATGCTGTGATTTTCGCGCGACTTAGTCACT 
               
               
                 ACGCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 90 
               
               
                 (K5R) 
               
               
                 ATACGGGAGCCAACACCAACGCACTAGTCCGTTCTCACTGTATAGCTG 
               
               
                 CCGCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 91 
               
               
                 (K7R) 
               
               
                 ATACGGGAGCCAACACCAGTCTATCCAGATCCATCATCGCTAGTATTA 
               
               
                 TTTGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 92 
               
               
                 (K8R) 
               
               
                 ATACGGGAGCCAACACCAGCATCGTCTTTCCCACTTACCCTAAGTCGC 
               
               
                 ACCCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 93 
               
               
                 (K9R) 
               
               
                 ATACGGGAGCCAACACCACCGCCTGTCGGACTAAGATTGGTTCATCTC 
               
               
                 CTCTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 94 
               
               
                 (K10R) 
               
               
                 ATACGGGAGCCAACACCACAGGAGGTGTAGGTGACGTCATGACAATT 
               
               
                 CCGGGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 95 
               
               
                 (Whole LPS from  E. coli  O111: B4(L)1F) 
               
               
                 ATCCGTCACCCCTGCTCTCGTCGCTATGAAGTAACAAAGATAGGAGCA 
               
               
                 ATCGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 96 
               
               
                 (L3F) 
               
               
                 ATCCGTCACACCTGCTCTAACGAAGACTGAAACCAAAGCAGTGACAG 
               
               
                 TGCTGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 97 
               
               
                 (L4F) 
               
               
                 ATCCGTCACACCTGCTCTCGGTGACAATAGCTCGATCAGCCCAAAGTC 
               
               
                 GTCAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 98 
               
               
                 (L6F) 
               
               
                 ATCCGTCACACCTGCTCTAACGAAATAGACCACAAATCGATACTTTAT 
               
               
                 GTTATTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 99 
               
               
                 (L7F) 
               
               
                 ATCCGTCACACCTGCTCTGTCGAATGCTCTGCCTGGAAGAGTTGTTAG 
               
               
                 CAGGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 100 
               
               
                 (L8F) 
               
               
                 ATCCGTCACACCTGCTCTTAAGCCGAGGGGTAAATCTAGGACAGGGGT 
               
               
                 CCATGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 101 
               
               
                 (L9F) 
               
               
                 ATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAAGAAGGAA 
               
               
                 GTTATGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 102 
               
               
                 (L10F) 
               
               
                 ATCCGTCACACCTGCTCTGGTACGAATCACAGGGGATGCTGGAAGCTT 
               
               
                 GGCTCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 103 
               
               
                 (L1R) 
               
               
                 ATACGGGAGCCAACACCACCCGATTGCTCCTATCTTTGTTACTTCATAG 
               
               
                 CGACGAGAGCAGGGGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 104 
               
               
                 (L3R) 
               
               
                 ATACGGGAGCCAACACCATTCAGCACTGTCACTGCTTTGGTTTCAGTC 
               
               
                 TTCGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 105 
               
               
                 (L4R) 
               
               
                 ATACGGGAGCCAACACCATCTGACGACTTTGGGCTGATCGAGCTATTG 
               
               
                 TCACCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 106 
               
               
                 (L6R) 
               
               
                 ATACGGGAGCCAACACCAATAACATAAAGTATCGATTTGTGGTCTATT 
               
               
                 TCGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 107 
               
               
                 (L7R) 
               
               
                 ATACGGGAGCCAACACCATCCCTGCTAACAACTCTTCCAGGCAGAGCA 
               
               
                 TTCGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 108 
               
               
                 (L8R) 
               
               
                 ATACGGGAGCCAACACCATCATGGACCCCTGTCCTAGATTTACCCCTC 
               
               
                 GGCTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 109 
               
               
                 (L9R) 
               
               
                 ATACGGGAGCCAACACCACATAACTTCCTTCTTAGTCATGCTGAGCCG 
               
               
                 GCCAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 110 
               
               
                 (L10R) 
               
               
                 ATACGGGAGCCAACACCAAGAGCCAAGCTTCCAGCATCCCCTGTGATT 
               
               
                 CGTACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 111 
               
               
                 (Rough (Ra or R) Core LPS Antigens R1F) 
               
               
                 ATCCGTCACACCTGCTCTCCGCACGTAGGACCACTTTGGTACACGCTC 
               
               
                 CCGTAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 112 
               
               
                 (R5F) 
               
               
                 ATCCGTCACACCTGCTCTACGGATGAACGAAGATTTTAAAGTCAAGCT 
               
               
                 AATGCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 113 
               
               
                 (R6F) 
               
               
                 ATCCGTCACACCTGCTCTGTAGTGAAGAGTCCGCAGTCCACGCTGTTC 
               
               
                 AACTCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 114 
               
               
                 (R7F) 
               
               
                 ATCCGTCACACCTGCTCTACCGGCTGGCACGGTTATGTGTGACGGGCG 
               
               
                 AAGATATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 115 
               
               
                 (R9F) 
               
               
                 ATCCGTCACACCTGCTCTGCGTGTGGAGCGCCTAGGTGAGTGGTGTTG 
               
               
                 GCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 116 
               
               
                 (R10F) 
               
               
                 ATCCGTCACACCTGCTCTGATGTCCCTTTGAAGAGTTCCATGACGCTGG 
               
               
                 CTCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 117 
               
               
                 (R1R) 
               
               
                 ATACGGGAGCCAACACCACTACGGGAGCGTGTACCAAAGTGGTCCTA 
               
               
                 CGTGCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 118 
               
               
                 (R5R) 
               
               
                 ATACGGGAGCCAACACCATGCATTAGCTTGACTTTAAAATCTTCGTTC 
               
               
                 ATCCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 119 
               
               
                 (R6R) 
               
               
                 ATACGGGAGCCAACACCATGAGTTGAACAGCGTGGACTGCGGACTCTT 
               
               
                 CACTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 120 
               
               
                 (R7R) 
               
               
                 ATACGGGAGCCAACACCATATCTTCGCCCGTCACACATAACCGTGCCA 
               
               
                 GCCGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 121 
               
               
                 (R9R) 
               
               
                 ATACGGGAGCCAACACCACTCACCTAGGCGCTCCACACGCAGAGCAG 
               
               
                 GTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 122 
               
               
                 (R10R) 
               
               
                 ATACGGGAGCCAACACCAAGGAGCCAGCGTCATGGAACTCTTCAAAG 
               
               
                 GGACATCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                   Enterococcus faecalis  Teichoic Acid (TA) Aptamers 
               
               
                 SEQ ID NO. 123 
               
               
                 (TA5F) 
               
               
                 CATTCACCACACCTCTGCTGGCTTGGCTAGCCTTGATGCTAAACGACCCA 
               
               
                 TAGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 124 
               
               
                 (TA5R) 
               
               
                 GATACGGGACGACACCACACTATGGGTCGTTTAGCATCAAGGCTAGCCAA 
               
               
                 GCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                 SEQ ID NO. 125 
               
               
                 (TA6F) 
               
               
                 CATTCACCACACCTCTGCTGGAGGAGGAAGTGGTCTGGAGTTACTTGACA 
               
               
                 TAGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 126 
               
               
                 (TA6R) 
               
               
                 GATACGGGACGACACCACACTATGTCAAGTAACTCCAGACCACTTCCTCC 
               
               
                 TCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                 SEQ ID NO. 127 
               
               
                 (TA7F) 
               
               
                 CATTCACCACACCTCTGCTGGACGGAAACAATCCCCGGGTACGAGAATCA 
               
               
                 GGGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 128 
               
               
                 (TA7R) 
               
               
                 GATACGGGACGACACCACACCCTGATTCTCGTACCCGGGGATTGTTTCCG 
               
               
                 TCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                 SEQ ID NO. 129 
               
               
                 (TA9F) 
               
               
                 CATTCACCACACCTCTGCTGGAAACCTACCATTAATGAGACATGATGCGG 
               
               
                 TGGTGTGGTGTCGTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 130 
               
               
                 (TA9R) 
               
               
                 GATACGGGACGACACCACACCACCGCATCATGTCTCATTAATGGTAGGTT 
               
               
                 TCCAGCAGAGGTGTGGTGAATG 
               
               
                   
               
               
                   Leishmania donovani  and  Leishmania tropica  Surface 
               
               
                 Protein Aptamers 
               
               
                 SEQ ID NO. 131 
               
               
                 (L-3F) 
               
               
                 GATACGGGAGCCAACACCACCCGTATCGTTCCCAATGCACTCAGAGCAGG 
               
               
                 TGTGACGGATG 
               
               
                   
               
               
                 SEQ ID NO. 132 
               
               
                 (L-3R) 
               
               
                 CATCCGTCACACCTGCTCTGAGTGCATTGGGAACGATACGGGTGGTGTTG 
               
               
                 GCTCCCGTATG 
               
               
                   
               
               
                 SEQ ID NO. 133 
               
               
                 (L-5F) 
               
               
                 GATACGGGAGCCAACACCACGTTCCCATACAAGTTACTGACAGAGCAGGT 
               
               
                 GTGACGGATG 
               
               
                   
               
               
                 SEQ ID NO. 134 
               
               
                 (L-5R) 
               
               
                 CATCCGTCACACCTGCTCTGTCAGTAACTTGTATGGGAACGTGGTGTTGG 
               
               
                 CTCCCGTATC 
               
               
                   
               
               
                   Bacillus anthraces  Poly-D-Glutamic Acid Capsule 
               
               
                 Aptamers 
               
               
                 SEQ ID NO. 135 
               
               
                 (PDGA 2F) 
               
               
                 CATCCGTCACACCTGCTCTGGTTCGCCCCGGTCAAGGAGAGTGGTGTTGG 
               
               
                 CTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 136 
               
               
                 (PDGA 2R) 
               
               
                 GATACGGGAGCCAACACCACTCTCCTTGACCGGGGCGAACCAGAGCAGGT 
               
               
                 GTGACGGATG 
               
               
                   
               
               
                 SEQ ID NO. 137 
               
               
                 (PDGA 5F) 
               
               
                 CATCCGTCACACCTGCTCTGGATAAGATCAGCAACAAGTTAGTGGTGTTG 
               
               
                 GCTCCCGTATC 
               
               
                   
               
               
                 SEQ ID NO. 138 
               
               
                 (PDGA 5R) 
               
               
                 GATACGGGAGCCAACACCACTAACTTGTTGCTGATCTTATCAGAGCAGGT 
               
               
                 GTGACGGATG 
               
               
                   
               
               
                   Organophosphorus  Pesticide Aptamers 
               
               
                 SEQ ID NO. 139 
               
               
                 (Diazinon(D)12F) 
               
               
                 ATACGGGAGCCAACACCATTAAATCAATTGTGCCGTGTTGGTCTTGTCTC 
               
               
                 ATCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 140 
               
               
                 (D12R) 
               
               
                 ATCCGTCACACCTGCTCTCGATGAGACAAGACCAACACGGCACAATTGAT 
               
               
                 TTAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 141 
               
               
                 (D17F) 
               
               
                 ATACGGGAGCCAACACCATTTTTATTATCGGTATGATCCTACGAGTTCCT 
               
               
                 CCCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 142 
               
               
                 (D17R) 
               
               
                 ATCCGTCACACCTGCTCTTGGGAGGAACTCGTAGGATCATACCGATAATA 
               
               
                 AAAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 143 
               
               
                 (D18F) 
               
               
                 ATACGGGAGCCAACACCACGTATATCTTATTATGCACAGCATCACGAA 
               
               
                 AGTGC-AGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 144 
               
               
                 (D18R) 
               
               
                 ATCCGTCACACCTGCTCTTTTTTATTATCGGTATGATCCTACGAGTTCCT 
               
               
                 CCCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 145 
               
               
                 (D19F) 
               
               
                 ATACGGGAGCCAACACCATTAACGTTAAGCGGCCTCACTTCTTTTAATCC 
               
               
                 TTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 146 
               
               
                 (D19R) 
               
               
                 ATCCGTCACACCTGCTCTGAAAGGATTAAAAGAAGTGAGGCCGCTTAACG 
               
               
                 TTAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 147 
               
               
                 (D20F) 
               
               
                 ATCCGTCACACCTGCTCTAATATAGAGGTATTGCTCTTGGACAAGGTACA 
               
               
                 GGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 148 
               
               
                 (D20R) 
               
               
                 ATACGGGAGCCAACACCATCCCTGTACCTTGTCCAAGAGCAATACCTCTA 
               
               
                 TATTACCACAACCGAGGGCATA 
               
               
                   
               
               
                 SEQ ID NO. 149 
               
               
                 (Malathion(M)17F) 
               
               
                 ATACGGGAGCCAACACCACAGTCAAGAAGTTAAGAGAAAAACAATTGTGT 
               
               
                 ATAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 150 
               
               
                 (M17R) 
               
               
                 ATCCGTCACACCTGCTCTTATACACAATTGTTTTTCTCTTAACTTCTTGA 
               
               
                 CTGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 151 
               
               
                 (M21F) 
               
               
                 ATCCGTCACACCTGCTCTGCGCCACAAGATTGCGGAAAGACACCCGGGGG 
               
               
                 GCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 152 
               
               
                 (M21R) 
               
               
                 ATACGGGAGCCAACACCAGCCCCCCGGGTGTCTTTCCGCAATCTTGTGGC 
               
               
                 GCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 153 
               
               
                 (M25F) 
               
               
                 ATCCGTCACACCTGCTCTGGCCTTATGTAAAGCGTTGGGTGGTGTTGGCT 
               
               
                 CCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 154 
               
               
                 (M25R) 
               
               
                 ATACGGGAGCCAACACCACCCAACGCTTTACATAAGGCCAGAGCAGGTGT 
               
               
                 GACGGAT 
               
               
                   
               
               
                 Foot-and-Mouth Disease (FMD) O-Serotype Viral 
               
               
                 Capsid Aptamers 
               
               
                 SEQ ID NO. 155 
               
               
                 (FMD 1F) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCITGCCAT 
               
               
                 CTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 156 
               
               
                 (FMD 1R) 
               
               
                 ATCCGTCACTCCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACGAT 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 157 
               
               
                 (FMD 10F) 
               
               
                 ATACGGGAGCCAACACCATGAATATCTCTTCTACCTCCTCTCCTCCCTT 
               
               
                 TACTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 158 
               
               
                 (FMD 10R) 
               
               
                 ATCCGTCACTCCTGCTCTAAGTAAAGGGAGGAGAGGAGGTAGAAGAGATA 
               
               
                 TTCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 159 
               
               
                 (FMD 11F) 
               
               
                 ATACGGGAGCCAACACCACGCCGCCCCAGTTCATGGCCTCTATGTCCGGC 
               
               
                 AACGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 160 
               
               
                 (FMD 11R) 
               
               
                 ATCCGTCACTCCTGCTCTCGTTGCCGGACATAGAGGCCATGAACTGGGGC 
               
               
                 GGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 161 
               
               
                 (FMD 12F) 
               
               
                 ATACGGGAGCCAACACCATCTAGATCTGAAGAATAAAACAAAGACAAAGA 
               
               
                 TGCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 162 
               
               
                 (FMD 12R) 
               
               
                 ATCCGTCACTCCTGCTCTAGCATCTTTGTCTTTGTTTTATTCAGATCTAG 
               
               
                 ATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 163 
               
               
                 (FMD 13F) 
               
               
                 ATACGGGAGCCAACACCACCTTTTAAAACGCTAGCTAGCTTAGTCCATTC 
               
               
                 CACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 164 
               
               
                 (FMD 13R) 
               
               
                 ATCCGTCACTCCTGCTCTGGTGGAATGGACTAAGCTAGCTAGCGTTTTAA 
               
               
                 AAGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 Aptamer Sequences Against Markers of Bone 
               
               
                 Resorption or Formation 
               
               
                 SEQ ID NO. 165 
               
               
                 (Hydroxylysine 5F) 
               
               
                 ATACGGGAGCCAACACCACGCTTAGATATTATCCTTGTCCAGAGCAGG 
               
               
                 TGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 166 
               
               
                 (Hydroxylysine 5R) 
               
               
                 ATCCGTCACACCTGCTCTGGACAAGGATAATATCTAAGCGTGGTGTTG 
               
               
                 GCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 167 
               
               
                 (Hydroxylysine 6F) 
               
               
                 ACACGGGAGCCAACACCATCCATAGCTCATCTATACCCTCTTCCGAGT 
               
               
                 CCCACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 168 
               
               
                 (Hydroxylysine 6R) 
               
               
                 ATCCGTCACACCTGCTCTGGTGGGACTCGGAAGAGGGTATAGATGAGC 
               
               
                 TATGGATGGTGTTGGCTCCCGTGT 
               
               
                   
               
               
                 SEQ ID NO. 169 
               
               
                 (Hydroxylysine 7F) 
               
               
                 ATACGGGAGCCAACACCACCCTACACCAGCGCCCTACACTTTTGTAGC 
               
               
                 ACTTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 170 
               
               
                 (Hydroxylysine 7R) 
               
               
                 ATCCGTCACACCTGCTCTCGAAGTGCTACAAAAGTGTAGGGCGCTGGT 
               
               
                 GTAGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 171 
               
               
                 (Hydroxylysine 8F) 
               
               
                 ATACGGGAGCCAACACCATAGTGTTGGGCCAATACGGTAACGTGTCCT 
               
               
                 TGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 172 
               
               
                 (Hydroxylysine 8R) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGCCCAACA 
               
               
                 CTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 173 
               
               
                 (Hydroxylysine 9F) 
               
               
                 ATACGGGAGCCAACACCACCCGTTTTTGATCTAATGAGGATACAATAT 
               
               
                 TCGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 174 
               
               
                 (Hydroxylysine 9R) 
               
               
                 ATCCGTCACACCTGCTCTAGACGAATATTGTATCCTCATTAGATCAAA 
               
               
                 AACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 175 
               
               
                 (Hydroxylysine 10F) 
               
               
                 ATACGGGAGCCAACACCAGCTTTTCCTAGAATGATTTTCTTTAGCTACC 
               
               
                 TGAGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 176 
               
               
                 (Hydroxylysine 10R) 
               
               
                 ATCCGTCACACCTGCTCTTCTCAGGTAGCTAAAGAAAATCATTCTAGG 
               
               
                 AAAAGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 177 
               
               
                 (Osteocalcin 2F) 
               
               
                 ATACGGGAGCCAACACCACGATTAGCAATGAATTATCTACAGAGCAG 
               
               
                 GTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 178 
               
               
                 (Osteocalcin 2R) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATAATTCATTGCTAATCGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 179 
               
               
                 (Osteocalcin 4F) 
               
               
                 ATACGGGAGCCAACACCAATTCTAACACAGGTTTCTCCGTTTCGTTAG 
               
               
                 CTGCTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 180 
               
               
                 (Osteocalcin 4R) 
               
               
                 ATCCGTCACACCTGCTCTTAGCAGCTAACGAAACGGAGAAACCTGTGT 
               
               
                 TAGAATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 181 
               
               
                 (Osteocalcin 7F) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCC 
               
               
                 ATCTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 182 
               
               
                 (Osteocalcin 7R) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACG 
               
               
                 ATAGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 183 
               
               
                 (Osteocalcin 8F) 
               
               
                 ATACGGGAGCCAACACCAACTGACTCAGTCTGCTGGTGGGCTATATTT 
               
               
                 TTGCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 184 
               
               
                 (Osteocalcin 8R) 
               
               
                 ATCCGTCACACCTGCTCTCCGCAAAAATATAGCCCACCAGCAGACTGA 
               
               
                 GTCAGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 185 
               
               
                 (ProCathepsin K 1F) 
               
               
                 ATACGGGAGCCAACACCATATAGCCGCGCCTGTGAGTTTTGTGGGAGC 
               
               
                 AAGAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 186 
               
               
                 (ProCathepsin K 1R) 
               
               
                 ATCCGTCACACCTGCTCTACTCTTGCTCCCACAAAACTCACAGGCGCG 
               
               
                 GCTATATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 187 
               
               
                 (ProCathepsin K 2F) 
               
               
                 ATACGGGAGCCAACACCAGCTACAGTGTCAGACGGTTCCACCTTAACC 
               
               
                 TCGTCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 188 
               
               
                 (ProCathepsin K 2R) 
               
               
                 ATCCGTCACACCTGCTCTTGACGAGGTTAAGGTGGAACCGTCTGACAC 
               
               
                 TGTAGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 189 
               
               
                 (ProCathepsin K 3F) 
               
               
                 ATACGGGAGCCAACACCATTGACTAAGCGATTAGTCCCACAGGTGAC 
               
               
                 CGGGGAGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 190 
               
               
                 (ProCathepsin K 3R) 
               
               
                 ATCCGTCACACCTGCTCTCTTCCCGGNCTCCTGTGTGATTAATCTGTTA 
               
               
                 TTCTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 191 
               
               
                 (ProCathepsin K 4F) 
               
               
                 ATACGGGAGCCAACACCAATTCTAACACAGGTTTCTCCGTTTCGTTAG 
               
               
                 CTGCTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 192 
               
               
                 (ProCathepsin K 4R) 
               
               
                 ATCCGTCACACCTGCTCTTAGCAGCTAACGAAACGGAGAAACCTGTGT 
               
               
                 TAGAATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 193 
               
               
                 (ProCathepsin K 6F) 
               
               
                 ATACGGGAGCCAACACCACTCATCCCGTTGGAACACTTTAATATGGCC 
               
               
                 CACTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 194 
               
               
                 (ProCathepsin K 6R) 
               
               
                 ATCCGTCACACCTGCTCTAGAGTGGGCCATATTAAAGTGTTCCAACGG 
               
               
                 GATGAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 195 
               
               
                 (C-Terminal Telopeptide of Human Collagen (CTx) 
               
               
                 1F) 
               
               
                 ATACGGGAGCCAACACCACTAACTTGTTGCTGATCTTATCCAGAGCAG 
               
               
                 GTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 196 
               
               
                 (CTx 1R) 
               
               
                 ATCCGTCACACCTGCTCTGGATAAGATCAGCAACAAGTTAGTGGTGTT 
               
               
                 GGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 197 
               
               
                 (CTx 2F) 
               
               
                 ATACGGGAGCCAACACCACCCGTTTTTGATCTAATGAGGATACAATAT 
               
               
                 TCGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 198 
               
               
                 (CTx 2R) 
               
               
                 ATCCGTCACACCTGCTCTAGACGAATATTGTATCCTCATTAGATCAAA 
               
               
                 AACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 199 
               
               
                 (CTx 3F) 
               
               
                 ATACGGGAGCCAACACCAATCGATGGTTAGACTATTACACTAGATGGA 
               
               
                 ATTCATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 200 
               
               
                 (CTx 3R) 
               
               
                 ATCCGTCACACCTGCTCTATGAATTCCATCTAGTGTAATAGTCTAACCA 
               
               
                 TCGATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 201 
               
               
                 (CTx 6F) 
               
               
                 ATACGGGAGCCAACACCAATCTGCCGACTAGGCCAAGTAATTATATTC 
               
               
                 AGCTGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 202 
               
               
                 (CTx 6R) 
               
               
                 ATCCGTCACACCTGCTCTCCAGCTGAATATAATTACTTGGCCTAGTCGG 
               
               
                 CAGATTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 203 
               
               
                 (CTx 7F) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCA 
               
               
                 GGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 204 
               
               
                 (CTx 7R) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTT 
               
               
                 GGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 205 
               
               
                 (CTx 8F) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCA 
               
               
                 GGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 206 
               
               
                 (CTx 8R) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTT 
               
               
                 GGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 207 
               
               
                 (CTx 11F) 
               
               
                 ATACGGGAGCCAACACCACATTACAATAGATGTATTGACATATCCGGA 
               
               
                 CAGTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 208 
               
               
                 (CTx 11R) 
               
               
                 ATCCGTCACACCTGCTCTCGACTGTCCGGATATGTCAATACATCTATTG 
               
               
                 TAATGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 209 
               
               
                 (CTx 13F) 
               
               
                 ATACGGGAGCCAACACCACCCGTTTTTGATCTAATGAGGATACAATAT 
               
               
                 TCGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 210 
               
               
                 (CTx 13R) 
               
               
                 ATCCGTCACACCTGCTCTAGACGAATATTGTATCCTCATTAGATCAAA 
               
               
                 AACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 211 
               
               
                 (CTx 14F) 
               
               
                 ATACGGGAGCCAACACCACTCGTGTAGTGCTGTCTTTGTGGAATCCTT 
               
               
                 GCATCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 212 
               
               
                 (CTx 14R) 
               
               
                 ATCCGTCACACCTGCTCTCGATGCAAGGATTCCACAAAGACAGCACTA 
               
               
                 CACGAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 213 
               
               
                 (CTx 15F) 
               
               
                 ATACGGGAGCCAACACCACCACGTGACCCATACGATACAACAAATAA 
               
               
                 TTGCTCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 214 
               
               
                 (CTx 15R) 
               
               
                 ATCCGTCACACCTGCTCTTGAGCAATTATTTGTTGTATCGTATGGGTCA 
               
               
                 CGTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 215 
               
               
                 (CTx 16F) 
               
               
                 ATACGGGAGCCAACACCATCCATAGCTCATCTATACCCTCTTCCGAGT 
               
               
                 CCCACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 216 
               
               
                 (CTx 16R) 
               
               
                 ATCCGTCACACCTGCTCTGGTGGGACTCGGAAGAGGGTATAGATGAGC 
               
               
                 TATGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 217 
               
               
                 (CTx 17F) 
               
               
                 ATACGGGAGCCAACACCAGACGCGGAACGACTCATCGCAAAATGTCG 
               
               
                 TGATGCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 218 
               
               
                 (CTx 17R) 
               
               
                 ATCCGTCACACCTGCTCTTGCATCACGACATTTTGCGATGAGTCGTTCC 
               
               
                 GCGTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 219 
               
               
                 (CTx 18F) 
               
               
                 ATACGGGAGCCAACACCATGGTTAGGCTGCTCCATATATTCCCGCCCC 
               
               
                 GCACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 220 
               
               
                 (CTx 18R) 
               
               
                 ATCCGTCACACCTGCTCTACGTGCGGGGCGGGAATATATGGAGCAGCC 
               
               
                 TAACCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 221 
               
               
                 (CTx 19F) 
               
               
                 ATACGGGAGCCAACACCACCCGTTTTTGATCTAATGAGGATACAATAT 
               
               
                 TCGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 222 
               
               
                 (CTx 19R) 
               
               
                 ATCCGTCACACCTGCTCTAGACGAATATTGTATCCTCATTAGATCAAA 
               
               
                 AACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 223 
               
               
                 (CTx 20F) 
               
               
                 ATACGGGAGCCAACACCACCCGTTTTTGATCTTATGAGGATACAATAT 
               
               
                 TCGTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 224 
               
               
                 (CTx 20R) 
               
               
                 ATCCGTCACACCTGCTCTAGACGAATATTGTATCCTCATAAGATCAAA 
               
               
                 AACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 225 
               
               
                 (N-Terminal Telopeptide of Human Collagen(NTx) 
               
               
                 2F) 
               
               
                 ATCCGTCACACCTGCTCTCCGACCAATGTGTGGATCATTACTAATCGACT 
               
               
                 ATTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 226 
               
               
                 (NTx 2R) 
               
               
                 ATACGGGAGCCAACACCACAATAGTCGATTAGTAATGATCCACACATTGG 
               
               
                 TCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 227 
               
               
                 (NTx 4F) 
               
               
                 ATACGGGAGCCAACACCATAGTTTTGGGCCAATACGGTAACGTGTCCTTG 
               
               
                 GAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 228 
               
               
                 (NTx 4R) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGCCCAAAACT 
               
               
                 ATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 229 
               
               
                 (NTx 8F) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGTCCAGCACT 
               
               
                 ATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 230 
               
               
                 (NTx 8R) 
               
               
                 ATACGGGAGCCAACACCATAGTGCTGGACCAATACGGTAACGTGTCCTTG 
               
               
                 GAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 231 
               
               
                 (NTx 10F) 
               
               
                 ATCCGTCACACCTGCTCTAACGTGTGGGTTGAAGTGTCGCCAACAAATTG 
               
               
                 ATAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 232 
               
               
                 (NTx 10R) 
               
               
                 ATACGGGAGCCAACACCACTATCAATTTGTTGGCGACACTTCAACCCACA 
               
               
                 CGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 233 
               
               
                 (NTx 11F) 
               
               
                 ATCCGTCACACCTGCTCTCGACCAAATATTTCCCCCAGCTCTAACCCATG 
               
               
                 CTGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 234 
               
               
                 (NTx 11R) 
               
               
                 ATACGGGAGCCAACACCATCAGCATGGGTTAGAGCTGGGGGAAATATTTG 
               
               
                 GTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 235 
               
               
                 (NTx 12F) 
               
               
                 ATACGGGAGCCAACACCATAGTGTTGGGCCAATACGGTAACGTGTCCTTG 
               
               
                 GAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 236 
               
               
                 (NTx 12R) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGCCCAACACT 
               
               
                 ATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 237 
               
               
                 (NTx 13F) 
               
               
                 ATCCGTCACACCTGCTCTGGGGTCCGCTTGGGAACGATATTCCTGTTGTT 
               
               
                 TTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 238 
               
               
                 (NTx 13R) 
               
               
                 ATACGGGAGCCAACACCAACAAAACAACAGGAATATCGTTCCCAAGCGGA 
               
               
                 CCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 239 
               
               
                 (NTx 14F) 
               
               
                 ATCCGTCACACCTGCTCTGATGGCAACATGGGTTAAATCTAACAACACTT 
               
               
                 TGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 240 
               
               
                 (NTx 14R) 
               
               
                 ATACGGGAGCCAACACCATACAAAGTGTTGTTAGATTTAACCCATGTTGC 
               
               
                 CATCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 241 
               
               
                 (NTx 15F) 
               
               
                 ATACGGGAGCCAACACCAAGGGTGTTCACACTGGCAGGCGACGCCCTCGT 
               
               
                 GTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 242 
               
               
                 (NTx 15R) 
               
               
                 ATCCGTCACACCTGCTCTCAACACGAGGGCGTCGCCTGCCAGTGTGAACA 
               
               
                 CCCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 243 
               
               
                 (25-Hydroxy-Vitamin D3 (Calcidiol) VD3 1F) 
               
               
                 ATACGGGAGCCAACACCATAGACAATGGCGTACTTTTCGTAATTCCAC 
               
               
                 AAGAATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 244 
               
               
                 (VD3 1R) 
               
               
                 ATCCGTCACACCTGCTCTATTCTTGTGGAATTACGAAAAGTACGCCATT 
               
               
                 GTCTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 245 
               
               
                 (VD3 2F) 
               
               
                 ATACGGGAGCCAACACCACCACAAAAGCATTCGCCCTTACAGAGCAG 
               
               
                 GTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 246 
               
               
                 (VD3 2R) 
               
               
                 ATCCGTCACACCTGCTCTGTAAGGGCGAATGCTTTTGTGGTGGTGTTG 
               
               
                 GCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 247 
               
               
                 (VD3 3F) 
               
               
                 ATACGGGAGCCAACACCAGCGTGTAGCTAGTTTCAGGATTGTAGTATG 
               
               
                 TAATATAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 248 
               
               
                 (VD3 3R) 
               
               
                 ATCCGTCACACCTGCTCTATATTACATACTACAATCCTGAAACTAGCTA 
               
               
                 CACGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 249 
               
               
                 (VD3 5F) 
               
               
                 ATACGGGAGCCAACACCACGCACATACTAGCTATCTCATCAGAGCAG 
               
               
                 GTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 250 
               
               
                 (VD3 5R) 
               
               
                 ATCCGTCACACCTGCTCTGATGAGATAGCTAGTATGTGCGTGGTGTTG 
               
               
                 GCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 251 
               
               
                 (VD3 6F) 
               
               
                 ATACGGGAGCCAACACCATCAGAGATCATCTAACGAAAATCATGGGT 
               
               
                 CTCGCCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 252 
               
               
                 (VD3 6R) 
               
               
                 ATCCGTCACACCTGCTCTGGGCGAGACCCATGATTTTCGTTAGATGAT 
               
               
                 CTCTGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 253 
               
               
                 (VD3 7F) 
               
               
                 ATACGGGAGCCAACACCAGCAAAGAATAGTGAGCCCTATGATCATCT 
               
               
                 GTTCGTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 254 
               
               
                 (VD3 7R) 
               
               
                 ATCCGTCACACCTGCTCTGACGAACAGATGATCATAGGGCTCACTATT 
               
               
                 CTTTGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 255 
               
               
                 (VD3 8F) 
               
               
                 ATACGGGAGCCAACACCAGACATCATGTCGCATATCTGGATCTAGAGG 
               
               
                 CTATTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 256 
               
               
                 (VD3 8R) 
               
               
                 ATCCGTCACACCTGCTCTGAATAGCCTCTAGATCCAGATATGCGACAT 
               
               
                 GATGTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 257 
               
               
                 (VD3 10F) 
               
               
                 ATACGGGAGCCAACACCAGTACGGCGGTGTCCGAACTCACTATACCC 
               
               
                 AGTTGAAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 258 
               
               
                 (VD3 10R) 
               
               
                 ATCCGTCACACCTGCTCTTTCAACTGGGTATAGTGAGTTCGGACACCG 
               
               
                 CCGTACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 259 
               
               
                 (VD3 13F) 
               
               
                 ATACGGGAGCCAACACCAGACCTGACAACGAAAACCCCAGTTGTCGC 
               
               
                 CATAGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 260 
               
               
                 (VD3 13R) 
               
               
                 ATCCGTCACACCTGCTCTGGCTATGGCGACAACTGGGGTTTTCGTTGTC 
               
               
                 AGGTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 261 
               
               
                 (VD3 14F) 
               
               
                 ATACGGGAGCCAACACCATAGTGTTGGGCCAATACGGTAACGTGTCCT 
               
               
                 TGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 262 
               
               
                 (VD3 14R) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGCCCAACA 
               
               
                 CTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 263 
               
               
                 (VD3 15F) 
               
               
                 ATACGGGAGCCAACACCATAAGCGCAACACAGTCCATCCCTGAGTGA 
               
               
                 GATAGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 264 
               
               
                 (VD3 15R) 
               
               
                 ATCCGTCACACCTGCTCTCGCTATCTCACTCAGGGATGGACTGTGTTGC 
               
               
                 GCTTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 265 
               
               
                 (VD3 16F) 
               
               
                 ATACGGGAGCCAACACCACGCACATACTAGCTATCTCATCAGAGCAG 
               
               
                 GTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 266 
               
               
                 (VD3 16R) 
               
               
                 ATCCGTCACACCTGCTCTGATGAGATAGCTAGTATGTGCGTGGTGTTG 
               
               
                 GCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 267 
               
               
                 (VD3 17F) 
               
               
                 ATACGGGAGCCAACACCACTAACTTGTTGCTGATCTTACCAGAGCAGG 
               
               
                 TGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 268 
               
               
                 (VD3 17R) 
               
               
                 ATCCGTCACACCTGCTCTGGTAAGATCAGCAACAAGTTAGTGGTGTTG 
               
               
                 GCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 269 
               
               
                 (VD3 18F) 
               
               
                 ATACGGGAGCCAACACCACCCGTTTTTGATCTAATGAGGATACAATAT 
               
               
                 TCGTCNAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 270 
               
               
                 (VD3 18R) 
               
               
                 ATCCGTCACACCTGCTCTNGACGAATATTGTATCCTCATTAGATCAAA 
               
               
                 AACGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 271 
               
               
                 (VD3 19F) 
               
               
                 ATACGGGAGCCAACACCAGTTGTGGGAACATCAGGCTAAGTATGAGA 
               
               
                 CGGAACGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 272 
               
               
                 (VD3 19R) 
               
               
                 ATCCGTCACACCTGCTCTCGTTCCGTCTCATACTTAGCCTGATGTTCCC 
               
               
                 ACAACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 273 
               
               
                 (VD3 20F) 
               
               
                 ATACGGGAGCCAACACCATAGTGTTGGGCCAATACGGTAACGTGTCCT 
               
               
                 TGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 274 
               
               
                 (VD3 20R) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGCCCAACA 
               
               
                 CTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 Botulinum Toxin Type A Aptamer Sequences 
               
               
                 SEQ ID NO. 275 
               
               
                 (Botulinum Toxin Type A-Light Chain (BoNT A-LC1)) 
               
               
                 CATCCGTCACACCTGCTCTGGGGATGTGTGGTGTTGGCTCCCGTATCAAG 
               
               
                 GGCGAATTCT 
               
               
                   
               
               
                 SEQ ID NO. 276 
               
               
                 (BoNT A-LC2) 
               
               
                 GTAGGCAGTGTGGACGAGACCCCTACACACCACAACCGAGGGCATAGTTC 
               
               
                 CCGCTTAAGA 
               
               
                   
               
               
                 SEQ ID NO.277 
               
               
                 (Botulinum Toxin Type A-Holotoxin (BoNT A-HT1)) 
               
               
                 CATCCGTCACACCTGCTCTGCTATCACATGCCTGCTGAAGTGGTGTTGGC 
               
               
                 TCCCGTATCA 
               
               
                   
               
               
                 SEQ ID NO. 278 
               
               
                 (BoNT A-HT2) 
               
               
                 GTAGGCAGTGTGGACGAGACGATAGTGTACGGACGACTTCACCACAACCG 
               
               
                 AGGGCATAGT 
               
               
                   
               
               
                   E. coli  Outer Membrane Proteins (OMPs) 
               
               
                 SEQ ID NO. 279 
               
               
                 (EcO-1F) 
               
               
                 ATCCGTCACACCTGCTCTCGATGTCTGGGCCCTAATATTGGTTTGCTTGT 
               
               
                 ACCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 280 
               
               
                 (EcO-1R) 
               
               
                 ATACGGGAGCCAACACCATGGTACAAGCAAACCAATATTAGGGCCCAGAC 
               
               
                 ATCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 281 
               
               
                 (EcO-2F) 
               
               
                 ATACGGGAGCCAACACCATGATACCCTAAGGTAGGGGAGGCCTAAGCGCC 
               
               
                 ACGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 282 
               
               
                 (EcO-2R) 
               
               
                 ATCCGTCACACCTGCTCTACGTGGCGCTTAGGCCTCCCCTACCTTAGGGT 
               
               
                 ATCATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 283 
               
               
                 (EcO-3F) 
               
               
                 ATACGGGAGCCAACACCACGCATCCCCCGCCGGGCCCGCGCCCCGCTCGC 
               
               
                 AGACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 284 
               
               
                 (EcO-3R) 
               
               
                 ATCCGTCACACCTGCTCTGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGA 
               
               
                 TGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 285 
               
               
                 (EcO-4F (73)) 
               
               
                 ATCCGTCACACCTGCTCTACGGCGCTCCCAACAGGCCTCTCCTTACGGCA 
               
               
                 TATTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 286 
               
               
                 (EcO-4R (73)) 
               
               
                 ATACGGGAGCCAACACCATAATATGCCGTAAGGAGAGGCCTGTTGGGAGC 
               
               
                 GCCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 287 
               
               
                 (EcO-5F) 
               
               
                 ATACGGGAGCCAACACCAGGAAAAAAAGAGCCTGTGAAGATTGTAATATC 
               
               
                 AGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 288 
               
               
                 (EcO-5R) 
               
               
                 ATCCGTCACACCTGCTCTAACTGATATTACAATCTTCACAGGCTCTTTTT 
               
               
                 TTCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 289 
               
               
                 (EcO-7Fa) 
               
               
                 ATCCGTCACACCTGCTCTCGGAGGTAGACTAGGATTGCGGCGGGGGGTCA 
               
               
                 GGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 290 
               
               
                 (EcO-7Fb) 
               
               
                 ATACGGGAGCCAACACCACAAAAGCCTTACCTAACTGCCAACAATGAATA 
               
               
                 GCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 291 
               
               
                 (EcO-7Ra) 
               
               
                 ATCCGTCACACCTGCTCTTGCTATTCATTGTTGGCAGTTAGGTAAGGCTT 
               
               
                 TTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 292 
               
               
                 (EcO-7Rb) 
               
               
                 ATACGGGAGCCAACACCATACCTGACCCCCCGCCGCAATCCTAGTCTACC 
               
               
                 TCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 293 
               
               
                 (EcO-8F) 
               
               
                 ATACGGGAGCCAACACCACGACTAACACGACCGTTGGGGGGGGCTCGCGC 
               
               
                 GGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 294 
               
               
                 (EcO-8R) 
               
               
                 ATCCGTCACACCTGCTCTGCCCGCGCGAGCCCCCCCCAACGGTCGTGTTA 
               
               
                 GTCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 295 
               
               
                 (EcO-9F) 
               
               
                 ATACGGGAGCCAACACCAGTCCCCGCCCAGCCGTGAGCCGTACCCCCGCA 
               
               
                 CACCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 296 
               
               
                 (EcO-9R) 
               
               
                 ATCCGTCACACCTGCTCTGGTGTGCGGGGGTACGGCTCACGGCTGGGCGG 
               
               
                 GGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 297 
               
               
                 (EcO-10F) 
               
               
                 ATCCGTCACACCTGCTCTCAAGGTTGGGCCTGCAAGAGCAAAAACGGGGC 
               
               
                 GGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 298 
               
               
                 (EcO-10R) 
               
               
                 ATACGGGAGCCAACACCATCCCGCCCCGTTTTTGCTCTTGCAGGCCCAAC 
               
               
                 CTTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 299 
               
               
                 (EcO-11F) 
               
               
                 ATCCGTCACACCTGCTCTACTTGGCTTGCGACTATTATTCACAGGGCCAA 
               
               
                 AGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 300 
               
               
                 (EcO-11 R) 
               
               
                 ATACGGGAGCCAACACCAGTCTTTGGCCCTGTGAATAATAGTCGCAAGCC 
               
               
                 AAGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 301 
               
               
                 (EcO-12F (69)) 
               
               
                 ATACGGGAGCCAACACCATAGTGTTGGACCAATACGGTAACGTGTCCTTG 
               
               
                 GAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 302 
               
               
                 (EcO-12R (69)) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGTCCAACACT 
               
               
                 ATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 303 
               
               
                 (EcO-17F) 
               
               
                 ATCCGTCACACCTGCTCTTGGAATGTCGGTGTTTTTCCAATTCCTTGGGT 
               
               
                 CGTGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 304 
               
               
                 (EcO-17R) 
               
               
                 ATACGGGAGCCAACACCA CACGACCCAAGGAATTGGAAAAACACCGACA 
               
               
                 TTCCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 305 
               
               
                 (EcO-18F) 
               
               
                 ATCCGTCACACCTGCTCTGCGACGGCGACGCGGTCCGGGCGGGGGTGGAG 
               
               
                 GACGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 306 
               
               
                 (EcO-18R) 
               
               
                 ATACGGGAGCCAACACCACGTCCTCCACCCCCGCCCGGACCGCGTCGCCG 
               
               
                 TCGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 307 
               
               
                 (EcO-19Fa) 
               
               
                 ATACGGGAGCCAACACCAGAGGGTTCTAGGGTCACTTCCATGAGAATGGC 
               
               
                 TCACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 308 
               
               
                 (EcO-19Fb) 
               
               
                 ATCCGTCACACCTGCTCTGGCCTGGGGACGCGAGGGAGGCGGGGGGAGTC 
               
               
                 GTGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 309 
               
               
                 (EcO-19Ra) 
               
               
                 ATACGGGAGCCAACACCACCACGACTCCCCCCGCCTCCCTCGCGTCCCCA 
               
               
                 GGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 310 
               
               
                 (EcO-19Rb) 
               
               
                 ATCCGTCACACCTGCTCT GTGAGCCATTCTCATGGAAGTGACCCTAGAA 
               
               
                 CCCTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 311 
               
               
                 (EcO-20F) 
               
               
                 ATCCGTCACACCTGCTCTCACAGGGCCTCTTACTATACAGTTCTCCAGCG 
               
               
                 CTGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 312 
               
               
                 (EcO-20R) 
               
               
                 ATACGGGAGCCAACACCAGCAGCGCTGGAGAACTGTATAGTAAGAGGCCC 
               
               
                 TGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 313 
               
               
                 (EcO-21F) 
               
               
                 ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGA 
               
               
                 GAGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 314 
               
               
                 (EcO-21R) 
               
               
                 ATACGGGAGCCAACACCATCTCTCTTAGGATACAAAGCCAAACTGAGCCC 
               
               
                 GTGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 315 
               
               
                 (EcO-22F) 
               
               
                 ATACGGGAGCCAACACCAGGGGTGGCGAACATGGTATAACTTGATAAGTG 
               
               
                 TGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 316 
               
               
                 (EcO-22R) 
               
               
                 ATCCGTCACACCTGCTCTTCACACTTATCAAGTTATACCATGTTCGCCAC 
               
               
                 CCCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 317 
               
               
                 (EcO-23F) 
               
               
                 ATACGGGAGCCAACACCACTCCGACACCGGCCGCCGGCACCACCCACTCC 
               
               
                 CCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 318 
               
               
                 (EcO-23R) 
               
               
                 ATCCGTCACACCTGCTCTAGGGGGAGTGGGTGGTGCCGGCGGCCGGTGTC 
               
               
                 GGAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 319 
               
               
                 (EcO-24F) 
               
               
                 ATACGGGAGCCAACACCATCCGGCGCGCCCTCCTCCCCCACTGCTCCCCG 
               
               
                 CCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 320 
               
               
                 (EcO-24R) 
               
               
                 ATCCGTCACACCTGCTCTCGGGCGGGGAGCAGTGGGGGAGGAGGGCGCGC 
               
               
                 CGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 321 
               
               
                 (EcO-25F) 
               
               
                 ATACGGGAGCCAACACCATACGCAGAGGTCCCCTACCCAGGCCAGCCGGA 
               
               
                 TGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 322 
               
               
                 (EcO-25R) 
               
               
                 ATCCGTCACACCTGCTCTGGCATCCGGCTGGCCTGGGTAGGGGACCTCTG 
               
               
                 CGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 Shiga toxins (Shiga-like Toxin type 1; Stx-1) 
               
               
                 SEQ ID NO. 323 
               
               
                 (SH-2F) 
               
               
                 ATCCGTCACACCTGCTCTGGAGACATTAAAAACCGGAGTTTATTTATACC 
               
               
                 TTTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 324 
               
               
                 (SH-2R) 
               
               
                 ATACGGGAGCCAACACCAGAAAGGTATAAATAAACTCCGGTTTTTAATGT 
               
               
                 CTCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 325 
               
               
                 (SH-3F (59)) 
               
               
                 ATACGGGAGCCAACACCACTAACTTGTTGCTGATCTTATCCAGAGCAGGT 
               
               
                 GTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 326 
               
               
                 (SH-3R (59)) 
               
               
                 ATCCGTCACACCTGCTCTGGATAAGATCAGCAACAAGTTAGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 327 
               
               
                 (SH-4F (58)) 
               
               
                 ATCCGTCACACCTGCTCTGCATGGAGAGTTTTTTGGTCAGTGGTGTTGGC 
               
               
                 TCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 328 
               
               
                 (SH-4R (58)) 
               
               
                 ATACGGGAGCCAACACCACTGACCAAAAAACTCTCCATGCAGAGCAGGTG 
               
               
                 TGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 329 
               
               
                 (SH-6F (58)) 
               
               
                 ATACGGGAGCCAACACCACGTTAACGCGTAGCCTTTGGACAGAGCAGGTG 
               
               
                 TGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 330 
               
               
                 (SH-6R (58)) 
               
               
                 ATCCGTCACACCTGCTCTGTCCAAAGGCTACGCGTTAACGTGGTGTTGGC 
               
               
                 TCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 331 
               
               
                 (SH-8/21/23/24/25F (59)) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTTGG 
               
               
                 CTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 332 
               
               
                 (SH-8/21/23/24/25 Rev (59)) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCAGGT 
               
               
                 GTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 333 
               
               
                 (SH-9F) 
               
               
                 ATCCGTCACACCTGCTCTCGTCCGTCATTAAGTTCGGAGGCTGGCGGGTT 
               
               
                 GCGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 334 
               
               
                 (SH-9R) 
               
               
                 ATACGGGAGCCAACACCAACGCAACCCGCCAGCCTCCGAACTTAATGACG 
               
               
                 GACGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 335 
               
               
                 (SH-10F) 
               
               
                 ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCCAT 
               
               
                 CTACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 336 
               
               
                 (SH-10R) 
               
               
                 ATCCGTCACACCTGCTCTGTAGATGGCAAGGCATAAGCGTCCGGAACGAT 
               
               
                 AGAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 337 
               
               
                 (SH-11F) 
               
               
                 TCCGTCACACCTGCTCTAACTCTTACTACTTTGTTGCTATCACATTCAAC 
               
               
                 TGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 338 
               
               
                 (SH-11R) 
               
               
                 ATACGGGAGCCAACACCAACAGTTGAATGTGATAGCAACAAAGTAGTAAG 
               
               
                 AGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 339 
               
               
                 (SH-12 F(58)) 
               
               
                 ATCCGTCACACCTGCTCTGGCCTTTCACCAAGCGTCCTTGTGGTGTTGGC 
               
               
                 TCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 340 
               
               
                 (SH-12R (58)) 
               
               
                 ATACGGGAGCCAACACCACAAGGACGCTTGGTGAAAGGCCAGAGCAGGT 
               
               
                 GTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 341 
               
               
                 (SH-16F (58)) 
               
               
                 ATCCGTCACACCTGCTCTGGCACCGAGCACGGGAACCCAGTGGTGTTGGC 
               
               
                 TCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 342 
               
               
                 (SH-16R (58)) 
               
               
                 ATACGGGAGCCAACACCACTGGGTTCCCGTGCTCGGTGCCAGAGCAGGTG 
               
               
                 TGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 343 
               
               
                 (SH-17F (69)) 
               
               
                 ATACGGGAGCCAACACCATAGTGTTGGGCCAATACGGTAACGTGTCCTT 
               
               
                 GGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 344 
               
               
                 (SH-17R (69)) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGCCCAACACT 
               
               
                 ATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 345 
               
               
                 (SH-18F) 
               
               
                 ATCCGTCACACCTGCTCTACCCGATGCCGCCCCGGGATTGTTGTATGACC 
               
               
                 ATCTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 346 
               
               
                 (SH-18R) 
               
               
                 ATACGGGAGCCAACACCAAGATGGTCATACAACAATCCCGGGGCGGCATC 
               
               
                 GGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 347 
               
               
                 (SH-19F) 
               
               
                 ATACGGGAGCCAACACCACCCCATGAGTACACGTGAACGGACACAGCCTC 
               
               
                 CGGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 348 
               
               
                 (SH-19R) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGAGGCTGTGTCCGTTCACGTGTACTCAT 
               
               
                 GGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 349 
               
               
                 (SH-20F) 
               
               
                 ATCCGTCACACCTGCTCTTAACCATTCATTTCTTTTGTGGTATGACCGTT 
               
               
                 CGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 350 
               
               
                 (SH-20R) 
               
               
                 ATACGGGAGCCAACACCAGGCGAACGGTCATACCACAAAAGAAATGAATG 
               
               
                 GTTAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 351 
               
               
                 (SH-22F (58)) 
               
               
                 ATCCGTCACACCTGCTCTGGGGCTCTTTTCGTTAACCAGGTGGTGTTGGC 
               
               
                 TCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 352 
               
               
                 (SH-22R (58)) 
               
               
                 ATACGGGAGCCAACACCACCTGGTTAACGAAAAGAGCCCCAGAGCAGGTG 
               
               
                 TGACGGAT 
               
               
                   
               
               
                   S. typhimurium  ( S. enterica serovar Typhimurium   
               
               
                 type 13311) OMPs 
               
               
                 SEQ ID NO. 353 
               
               
                 (StO-2F) 
               
               
                 ATACGGGAGCCAACACCAGATAAATTTTGCGTTCATTCTTATTTCCTGT 
               
               
                 CCGCCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 354 
               
               
                 (StO-2R) 
               
               
                 ATCCGTCACACCTGCTCTGGCGGACAGGAAATAAGAATGAACGCAAA 
               
               
                 ATTTATCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 355 
               
               
                 (StO-4F) 
               
               
                 ATACGGGAGCCAACACCAGATAAATTTTGGTTCATTCTTATTTCCTGTC 
               
               
                 CGCCAGAGCAGGTGTGACGGAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 356 
               
               
                 (StO-4R) 
               
               
                 ATCCGTCACACCTGCTCTGGCGGACAGGAAATAAGAATGAACCAAAA 
               
               
                 TTTATCTGGTGTTGGCTCCCGTAT (71) 
               
               
                   
               
               
                 SEQ ID NO. 357 
               
               
                 (StO-5F) 
               
               
                 ATACGGGAGCCAACACCACGGGGCTACCAGCACCGTCACCCCTCATTC 
               
               
                 TGCCACAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 358 
               
               
                 (StO-5R) 
               
               
                 ATCCGTCACACCTGCTCTGTGGCAGAATGAGGGGTGACGGTGCTGGTA 
               
               
                 GCCCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 359 
               
               
                 (StO-6F) 
               
               
                 ATACGGGAGCCAACACCAAAAGATGGAAAACACTGGAAGGAAAATGC 
               
               
                 GGTCAGAGCAGGTGTGACGGAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 360 
               
               
                 (StO-6R) 
               
               
                 ATCCGTCACACCTGCTCTGACCGCATTTTCCTTCCAGTGTTTTCCATCTT 
               
               
                 TTGGTGTTGGCTCCCGTAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 361 
               
               
                 (StO-7F) 
               
               
                 ATACGGGAGCCAACACCACCGGGCCGATGGGCACCAGGAACTCTCGG 
               
               
                 ACGAGTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 362 
               
               
                 (StO-7R) 
               
               
                 ATCCGTCACACCTGCTCTCACTCGTCCGAGAGTTCCTGGTGCCCATCG 
               
               
                 GCCCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 363 
               
               
                 (StO-8F) 
               
               
                 ATACGGGAGCCAACACCACAGCTGATATTGGATGGTCCGGCAGAGCA 
               
               
                 GGTGTGACGGAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 364 
               
               
                 (StO-8R) 
               
               
                 ATCCGTCACACCTGCTCTGCCGGACCATCCAATATCAGCTGTGGTGTT 
               
               
                 GGCTCCCGTAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 365 
               
               
                 (StO-9F) 
               
               
                 ATACGGGAGCCAACACCAGTCGAAAGGCGGCCGTCCAGTCGAGTGAT 
               
               
                 TTGACCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 366 
               
               
                 (StO-9R) 
               
               
                 ATCCGTCACACCTGCTCTAGGTCAAATCACTCGACTGGACGGCCGCCT 
               
               
                 TTCGACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 367 
               
               
                 (StO-10F) 
               
               
                 ATACGGGAGCCAACACCACGGGGCGTGCCGTCAAAAGACCGAGATGT 
               
               
                 GGCTGCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 368 
               
               
                 (StO-10R) 
               
               
                 ATCCGTCACACCTGCTCTCGCAGCCACATCTCGGTCTTTTGACGGCAC 
               
               
                 GCCCCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 369 
               
               
                 (StO-11/13F) 
               
               
                 ATACGGGAGCCAACACCACTAACTTGTTGCTGATCTTATCCAGAGCAG 
               
               
                 GTGTGACGGAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 370 
               
               
                 (StO-11/13R) 
               
               
                 ATCCGTCACACCTGCTCTGGATAAGATCAGCAACAAGTTAGTGGTGTT 
               
               
                 GGCTCCCGTAT (59) 
               
               
                   
               
               
                 SEQ ID NO. 371 
               
               
                 (StO-12F) 
               
               
                 ATACGGGAGCCAACACCATTTAGCGTAGGGCTCGCTTATCATTTCTCA 
               
               
                 TTCCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 372 
               
               
                 (StO-12R) 
               
               
                 ATCCGTCACACCTGCTCTAGGGAATGAGAAATGATAAGCGAGCCCTAC 
               
               
                 GCTAAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 373 
               
               
                 (StO-14F) 
               
               
                 ATACGGGAGCCAACACCACCGCAACCCAAATCTCTACACGGATTATCG 
               
               
                 TCGAGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 374 
               
               
                 (StO-14R) 
               
               
                 ATCCGTCACACCTGCTCTGCTCGACGATAATCCGTGTAGAGATTTGGG 
               
               
                 TTGCGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 375 
               
               
                 (StO-16F) 
               
               
                 ATACGGGAGCCAACACCAACACATTCTATAATGAATGTTCCTGTCGCG 
               
               
                 TTGCGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 376 
               
               
                 (StO-16R) 
               
               
                 ATCCGTCACACCTGCTCTACGCAACGCGACAGGAACATTCATTATAGA 
               
               
                 ATGTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 377 
               
               
                 (StO-17F) 
               
               
                 ATACGGGAGCCAACACCAGCCTACCCCCCCTGTACGAGGGCCGCAAC 
               
               
                 CACGTAGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 378 
               
               
                 (StO-17R) 
               
               
                 ATCCGTCACACCTGCTCTCTACGTGGTTGCGGCCCTCGTACAGGGGGG 
               
               
                 GTAGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 379 
               
               
                 (StO-18F) 
               
               
                 ATACGGGAGCCAACACCACATCTAGCACGAGACCCTATCCCAGAGCA 
               
               
                 GGTGTGACGGAT(59) 
               
               
                   
               
               
                 SEQ ID NO. 380 
               
               
                 (StO-18R) 
               
               
                 ATCCGTCACACCTGCTCTGGGATAGGGTCTCGTGCTAGATGTGGTGTT 
               
               
                 GGCTCCCGTAT(59) 
               
               
                   
               
               
                 SEQ ID NO. 381 
               
               
                 (StO-19F) 
               
               
                 ATACGGGAGCCAACACCAACAGCGACTCGAGTCTGACGACTCGCGGG 
               
               
                 GCAAATGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 382 
               
               
                 (StO-19R) 
               
               
                 ATCCGTCACACCTGCTCTCATTTGCCCCGCGAGTCGTCAGACTCGAGT 
               
               
                 CGCTGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 383 
               
               
                 (StO-20/24F) 
               
               
                 ATACGGGAGCCAACACCATAGTGTTGGGCCAATACGGTAACGTGTCCT 
               
               
                 TGGAGAGCAGGTGTGACGGAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 384 
               
               
                 (StO-20/24R) 
               
               
                 ATCCGTCACACCTGCTCTCCAAGGACACGTTACCGTATTGGCCCAACA 
               
               
                 CTATGGTGTTGGCTCCCGTAT (69) 
               
               
                   
               
               
                 SEQ ID NO. 385 
               
               
                 (StO-21F) 
               
               
                 ATACGGGAGCCAACACCACTAAGGAGAGGTCGCGACAGACTCTTCTG 
               
               
                 GTCAAGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 386 
               
               
                 (StO-21R) 
               
               
                 ATCCGTCACACCTGCTCTCCTTGACCAGAAGAGTCTGTCGCGACCTCT 
               
               
                 CCTTAGTGGTGTTGGCTCCCGTATG 
               
               
                   
               
               
                 SEQ ID NO. 387 
               
               
                 (StO-22F) 
               
               
                 ATACGGGAGCCAACACCAACTTCGACTCAAAGAAGTCCACGTGAGAC 
               
               
                 TGGTGGAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 388 
               
               
                 (StO-22R) 
               
               
                 ATCCGTCACACCTGCTCTTCCACCAGTCTCACGTGGACTTCTTTGAGTC 
               
               
                 GAAGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 389 
               
               
                 (StO-23F) 
               
               
                 ATACGGGAGCCAACACCACCCGGGGAGACCCGCACGGGCGCACAATC 
               
               
                 CTTGTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 390 
               
               
                 (StO-23R) 
               
               
                 ATCCGTCACACCTGCTCTCGACAAGGATTGTGCGCCCGTGCGGGTCTC 
               
               
                 CCCGGGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 391 
               
               
                 (StO-25F) 
               
               
                 ATACGGGAGCCAACACCAGCTGGACCAAACTACGCCCATTGTGGGGG 
               
               
                 TCCCCGGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 392 
               
               
                 (StO-25R) 
               
               
                 ATCCGTCACACCTGCTCTCCGGGGACCCCCACAATGGGCGTAGTTTGGTC 
               
               
                 CAGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 Acetylcholine (ACh) 
               
               
                 SEQ ID NO. 393 
               
               
                 (ACh1aF) 
               
               
                 ATACGGGAGCCAACACCACGATACCCGCTTATGAATTTTAAATTAATTGT 
               
               
                 GATCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 394 
               
               
                 (ACh 1aR) 
               
               
                 ATCCGTCACACCTGCTCTGATCACAATTAATTTAAAATTCATAAGCGGGT 
               
               
                 ATCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 395 
               
               
                 (ACh 1bF) 
               
               
                 ATACGGGAGCCAACACCAACTTTCACACATACTTGTTATACCACACGATC 
               
               
                 TTTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 396 
               
               
                 (ACh 1bR) 
               
               
                 ATCCGTCACACCTGCTCTAAAAGATCGTGTGGTATAACAAGTATGTGTGA 
               
               
                 AAGTTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 397 
               
               
                 (ACh 2F) 
               
               
                 ATACGGGAGCCAACACCACTTTGTAACTCATTTGTAGTTTGGGTTGCTCC 
               
               
                 CCCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 398 
               
               
                 (ACh 2R) 
               
               
                 ATCCGTCACACCTGCTCTAGGGGGAGCAACCCAAACTACAAATGAGTTAC 
               
               
                 AAAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 399 
               
               
                 (ACh 3F) 
               
               
                 ATACGGGAGCCAACACCATTTCCCGCTTATCTTCATCCACTGCTTAGCAT 
               
               
                 ATGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 400 
               
               
                 (ACh 3R) 
               
               
                 ATCCGTCACACCTGCTCTACATATGCTAAGCAGTGGATGAAGATAAGCGG 
               
               
                 GAAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 401 
               
               
                 (ACh 5F) 
               
               
                 ATACGGGAGCCAACACCAGGCACTGTATCACACCGTCAAGAATGTGATCC 
               
               
                 CCTGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 402 
               
               
                 (ACh 5R) 
               
               
                 ATCCGTCACACCTGCTCTCAGGGGATCACATTCTTGACGGTGTGATACAG 
               
               
                 TGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 403 
               
               
                 (ACh 6F) 
               
               
                 ATACGGGAGCCAACACCATGTCATTTACCTTCATCATGACAGTGTTAGTA 
               
               
                 TACGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 404 
               
               
                 (ACh 6R) 
               
               
                 ATCCGTCACACCTGCTCTAGGGGATCAAAGCTATGCGACCATGCGAGTGG 
               
               
                 ATACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 405 
               
               
                 (ACh 7F) 
               
               
                 ATACGGGAGCCAACACCAGTTGCCGCCTACCTTGATTATTCTACATTACC 
               
               
                 CGTTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 406 
               
               
                 (ACh 7R) 
               
               
                 ATCCGTCACACCTGCTCTAACGGGTAATGTAGAATAATCAAGGTAGGCGG 
               
               
                 CAACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 407 
               
               
                 (ACh 8F) 
               
               
                 ATACGGGAGCCAACACCAGTATACATACGAAGAGTTGAAACCAATGCTTC 
               
               
                 GTTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 408 
               
               
                 (ACh 8R) 
               
               
                 ATCCGTCACACCTGCTCTGAACGAAGCATTGGTTTCAACTCTTCGTATGT 
               
               
                 ATACTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 409 
               
               
                 (ACh 9F) 
               
               
                 ATACGGGAGCCAACACCATACCCCGAATGGCTGTTTTCAGTACCAATATG 
               
               
                 ACTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 410 
               
               
                 (ACh 9R) 
               
               
                 ATCCGTCACACCTGCTCTGAGTCATATTGGTACTGAAAACAGCCATTCGG 
               
               
                 GGTATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 411 
               
               
                 (ACh 10F) 
               
               
                 ATACGGGAGCCAACACCACTGTCACGATCGTCGTTCCTTTTAATCCTTGT 
               
               
                 GTCTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 412 
               
               
                 (ACh 10R) 
               
               
                 ATCCGTCACACCTGCTCTAGACACAAGGATTAAAAGGAACGACGATCGTG 
               
               
                 ACAGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 413 
               
               
                 (ACh 11F) 
               
               
                 ATACGGGAGCCAACACCACTGGACACTGACCCTCGCACTAGCTTTCTGAC 
               
               
                 GGGTAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 414 
               
               
                 (ACh 11 R) 
               
               
                 ATCCGTCACACCTGCTCTACCCGGCCGAAGAATAGTGCTCGGTACTTAGT 
               
               
                 CGCGTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 415 
               
               
                 (ACh 12F) 
               
               
                 ATACGGGAGCCAACACCATTTGGACTTTAAATAGTGGACTCCTTCTTTGT 
               
               
                 CTCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 416 
               
               
                 (ACh 12R) 
               
               
                 ATCCGTCACACCTGCTCTCGAGACAAAGAAGGAGTCCACTATTTAAAGTC 
               
               
                 CAAATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 Gram Negative Quorum Sensing Molecules (N- 
               
               
                 Acylhomoserine Lactones; AHLs) 
               
               
                 SEQ ID NO. 417 
               
               
                 (Dec AHL 1F) 
               
               
                 ATACGGGAGCCAACACCATCCTAACTGGTCTAATTTTTGCTGTTACCGAT 
               
               
                 CCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 418 
               
               
                 (Dec AHL 1R) 
               
               
                 ATCCGTCACTCCTGCTCTCGGGATCGGTAACAGCAAAAATTAGACCAGTT 
               
               
                 AGGATGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 419 
               
               
                 (Dec AHL 13F) 
               
               
                 ATACGGGAGCCAACACCAGCCTGACGAAAAAATTTTATCACTAAGTGATA 
               
               
                 CGCAAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 420 
               
               
                 (Dec AHL 13R) 
               
               
                 ATCCGTCACACCTGCTCTTGCGTATCACTTAGTGATAAAATTTTTTCGTC 
               
               
                 AGGCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 421 
               
               
                 (Dec AHL 14F) 
               
               
                 ATACGGGAGCCAACACCAGACCTACTTCAGAAACGGAAATGTTCTTAGCC 
               
               
                 GTCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 422 
               
               
                 (Dec AHL 14R) 
               
               
                 ATCCGTCACACCTGCTCTGACGGCTAAGAACATTTCCGTTTCTGAAGTAG 
               
               
                 GTCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 423 
               
               
                 (Dec AHL 15F) 
               
               
                 ATACGGGAGCCAACACCAGGCCAACGAAACTCCTACTACATATAATGCTT 
               
               
                 ATGCAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 424 
               
               
                 (Dec AHL 15R) 
               
               
                 ATCCGTCACACCTGCTCTGCATAAGCATTATATGTAGTAGGAGTTTCGTT 
               
               
                 GGCCTGGTGTTGGCTCCCGTAT 
               
               
                   
               
               
                 SEQ ID NO. 425 
               
               
                 (Dec AHL 17F) 
               
               
                 ATACGGGAGCCAACACCATCCTAACTGGTCTAATTTTTGCTGTTACCGAT 
               
               
                 CCCGAGAGCAGGTGTGACGGAT 
               
               
                   
               
               
                 SEQ ID NO. 426 
               
               
                 (Dec AHL 17R) 
               
               
                 ATCCGTCACACCTGCTCTCGGGATCGGTAACAGCAAAAATTAGACCAGTT 
               
               
                 AGGATGGTGTTGGCTCCCGTAT