Abstract:
The present invention is to present an analyzer for analyzing a concentration of a predetermined component contained in tissue fluid of a subject, the analyzer being capable of mitigating the pain of the subject. The analyzer comprises: an extraction medium retainer for retaining an extraction medium for holding tissue fluid extracted through a skin of a subject; a component amount information obtainer for obtaining component amount information regarding amount of a predetermined component contained in the tissue fluid held in the extraction medium; an electrical information obtainer for obtaining electrical information related to amount of the extracted tissue fluid by supplying electrical power to the extraction medium which is holding the extracted tissue fluid; and a component concentration obtainer for obtaining a concentration of the predetermined component contained in body fluid of the subject based on the component amount information and the electrical information.

Description:
RELATED APPLICATIONS 
       [0001]    This application claims priority under 35 U.S.C. §119 to Japanese Patent Application No. JP2006-092708 filed Mar. 30, 2006, the entire content of which is hereby incorporated by reference. 
       FIELD OF THE INVENTION 
       [0002]    The present invention relates to a device and a method for measuring a concentration of a predetermined component contained in body fluid of a subject. 
       BACKGROUND OF THE INVENTION 
       [0003]    A method for measuring blood collected from a finger via a lancet mechanism using blood glucose test paper is disclosed as a blood glucose measuring method in U.S. Pat. No. 6,607,543. Furthermore, a device for executing this method is also commercially available. This device, however, is painful to the subject because blood is collected by piercing the finger of the subject. 
         [0004]    A glucose extraction method using reverse iontophoresis for transdermally extracting glucose by applying electrical energy to the skin is disclosed as a method for mitigating the pain of the subject in WO 96/00110. 
         [0005]    In order to confirm the correlation between amount of the extracted glucose and blood glucose level, however, blood must actually be collected to measure blood glucose level in the method disclosed in WO 96/00110. Therefore, the subject inevitably experiences pain due to blood collection. 
       SUMMARY OF THE INVENTION 
       [0006]    The first aspect of the present invention is an analyzer, comprising: an extraction medium retainer for retaining an extraction medium for holding tissue fluid extracted through a skin of a subject; a component amount information obtainer for obtaining component amount information regarding amount of a predetermined component contained in the tissue fluid held in the extraction medium; an electrical information obtainer for obtaining electrical information related to amount of the extracted tissue fluid by supplying electrical power to the extraction medium which is holding the extracted tissue fluid; and a component concentration obtainer for obtaining a concentration of the predetermined component contained in body fluid of the subject based on the component amount information and the electrical information. 
         [0007]    The second aspect of the present invention is an analyzer, comprising: a first electrode; a second electrode; an extraction medium retainer for retaining an extraction medium for holding tissue fluid extracted through the skin of a subject, the extraction medium contacting with the first electrode and the second electrode; an electrical power supplier for applying an electrical current between the first electrode and the second electrode; a first detector for detecting amount of a predetermined component contained in the tissue fluid held in the extraction medium; a second detector for detecting electrical information related to supply of electricity from the electrical power supplier; and a component concentration obtainer for obtaining a concentration of the predetermined component contained in body fluid of the subject based on the amount of the predetermined component detected by the first detector and the electrical information detected by the second detector. 
         [0008]    The third aspect of the present invention is an analyzing method, comprising steps of (a) extracting tissue fluid through the skin of a subject into an extraction medium for holding the extracted tissue fluid; (b) obtaining component amount information regarding amount of a predetermined component contained in the tissue fluid held in the extraction medium; (c) obtaining electrical information related to amount of the extracted tissue fluid by supplying electrical power to the extraction medium which is holding the extracted tissue fluid; and (d) obtaining a concentration of the predetermined component contained in body fluid of the subject based on the component amount information and the electrical information. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0009]      FIG. 1  is a perspective view of a blood glucose level measuring device  1  mounted on the wrist of a subject; 
           [0010]      FIG. 2  is a perspective view of a band  19  provided with a fixture  20 ; 
           [0011]      FIG. 3  shows the internal structure of the blood glucose level measuring device  1  installed with an extraction cartridge  2 ; 
           [0012]      FIG. 4  is a structural diagram showing the structure of the blood glucose level measuring device  1  of  FIG. 3 ; 
           [0013]      FIG. 5  is a top view showing the structure of the extraction cartridge  2 ; 
           [0014]      FIG. 6  is a cross section view along the VI-VI line of the extraction cartridge  2  shown in  FIG. 5 ; 
           [0015]      FIG. 7  is a perspective view of the extraction cartridge set  200 ; 
           [0016]      FIG. 8  is a cross section view along the VIII-VIII line of the extraction cartridge set  200  shown in  FIG. 7 ; 
           [0017]      FIG. 9  is a perspective view of the extraction cartridge set  200 ; 
           [0018]      FIG. 10  is a perspective view showing the micro needle array  51  used in a preparatory step; 
           [0019]      FIG. 11  shows the structure of the detector  30  for detecting glucose by illuminating a glucose sensor  70 ; 
           [0020]      FIG. 12  is a flow chart showing preparation sequence of the subject for measurement by the blood glucose level measuring device  1 ; 
           [0021]      FIG. 13  is a flow chart showing the measurement operation sequence of the controller-analyzer  11  of the blood glucose level measuring device  1 ; 
           [0022]      FIG. 14 to 16  and  FIG. 18  are schematic views illustrating the glucose extraction principle with the use of the blood glucose level measuring device  1 ; 
           [0023]      FIG. 17  shows the electrical circuit when an electric field is applied to the skin of the subject; 
           [0024]      FIG. 19  shows the electrical circuit when measuring the electrical conductance of the extraction medium; and 
           [0025]      FIG. 20  is a characteristics chart showing the relationship between the electrical conductance of the extraction medium and the glucose extraction speed. 
       
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     [General Structure of Blood Glucose Level Measuring Device  1 ] 
       [0026]    The embodiments of the present invention are described hereinafter based on the drawings. 
         [0027]    The general structure of the blood glucose level measuring device  1  including an installed extraction cartridge  2  is first described using  FIGS. 1 through 4 . The embodiment of the blood glucose level measuring device  1  of the present invention is a device for extracting tissue fluid containing glucose as a biochemical component from a body, and calculating blood glucose level by analyzing the glucose contained in the extracted tissue fluid. The tissue fluid is the intercellular fluid component of the tissue of animals. 
         [0028]    The subjects are mainly homo sapiens. 
         [0029]    As shown in  FIG. 1 , the blood glucose level measuring device  1  is configured so as to be mountable on the wrist of a subject using the band  19 . The blood glucose level measuring device  1  is provided with a hinge  7  that joins the analyzing unit  6  and fixture  20 , and the analyzing unit  6  is disposed so as to contact with the surface of the fixture  20  via the opening and closing operation of the hinge  7 .  FIG. 2  shows the band  19  when the analyzing unit  6  is removed from the blood glucose level measuring device  1 . As shown in  FIG. 2 , the fixture  20  of the band  19  is provided with an opening  20   a  at a predetermined position, and the opening  20   a  is configured such that the skin of the subject is exposed through the fixture  20 . Furthermore, an electrode  3  (not shown in the drawing) is attached to the back surface of the band  19 . A gold electrode is used as the electrode  3 . 
         [0030]    The blood glucose level measuring device  1  is provided with the analyzing unit  6 , extraction cartridge  2  removably fixed on the analyzing unit  6 , and the band  19 , as shown in  FIG. 4 . When the analyzing unit  6  with the fixed extraction cartridge  2  is installed on the fixture  20  of the band  19 , the extraction cartridge  2  fixed on the analyzing unit  6  contacts with the skin of the subject through the opening  20   a  of the fixture  20 . 
         [0031]    As shown in  FIG. 4 , the analyzing unit  6  is provided with a controller-analyzer  11 , switching circuit  12 , input part  14 , display (LCD)  15 , direct current type constant current power supplier  13 , voltmeter  16  for measuring the voltage supplied from the constant current power supplier  13  and outputting the voltage level to the controller-analyzer  11 , and a detector  30 . The analyzing unit  6  is installed on the fixture  20  of the band  19  and used to measure a blood glucose level. 
         [0032]    The controller-analyzer  11  is provided to control the output of the constant current power supplier  13  and the operation of the switching circuit  12 , and receive the output of the detector  30  and output of the voltmeter  16 , and calculate the blood glucose level. The controller-analyzer  11  is configured by a micro computer that includes a CPU, ROM, RAM and the like. 
         [0033]    The display (LCD)  15  is provided to display the calculation result (for example, blood glucose level) of the controller-analyzer  11 . 
         [0034]    The input part  14  is provided for inputting a measurement start command and necessary measuring conditions to the controller-analyzer  11 . 
         [0035]    The constant current power supplier  13  is provided for supplying a voltage between the electrode  3  provided on the band  19 , the electrode  4  and electrode  5  provided on the extraction cartridge  2 . The constant current power supplier  13  is configured by combining a transistor constant current circuit and a transistor constant voltage circuit with an internal battery. 
         [0036]    The switching circuit  12  is configured by an assembly of switching elements, and is provided for switching the output destination of the constant current power supplier  13 . Two terminals  12   b  and  12   c  (refer to  FIGS. 17 and 19 ) are connected to the switching circuit  12 , and the terminals  12   b  and  12   c  are provided on the surface of the analyzing unit  6  so as to contact with the electrodes  4  and  5  of the extraction cartridge  2  when the extraction cartridge  2  is fixed on the analyzing unit  6 . Furthermore, the switching circuit  12  is electrically connected to the electrode  3  and the constant current voltage  13  (refer to  FIG. 17 ). An inverter  12   a  is also connected to the switching circuit  12  to convert the direct current to an alternating current. 
         [0037]    The detector  30  is provided to detect the amount of extracted glucose. As shown in  FIG. 11 , the detector  30  is configured by a monochrome light source  31 , lens  32 , lens  33 , and photoreceptor  34 . The monochrome light source  31  functions to emit light for analysis to a glucose sensor  70  via the lens  32 . The light, which is emitted from the monochrome light source  31  through the lens  32  to the glucose sensor  70 , passes through a predetermined path of the glucose sensor  70 , and impinges the photoreceptor  34  through the lens  33 . The photoreceptor  34  functions to output a signal based on the amount of received light. 
         [0038]    As shown in  FIG. 5 , the extraction cartridge  2  is provided with a cartridge body  22  formed of acrylic rubber, electrode  4 , electrode  5 , masking tape  27 , mesh sheet  23 , and glucose sensor  70 . As shown in  FIG. 3 , the extraction cartridge  2  is removably fixed to the analyzing unit  6  by engaging each of the connector hooks  17  of the analyzing unit  6  with the two mounting holes  21  formed in the extraction cartridge  2 . The extraction cartridge  2  is configured so as to be capable of replaced and used for each glucose measurement. 
         [0039]    A square-shaped concave part  22   a  is provided on the extraction cartridge body  22 , as shown in  FIG. 5 . As shown in  FIGS. 5 and 6 , a through hole  22   b  that reaches to the bottom surface of the cartridge body  22  is formed in the center of the concave part  22   a . Furthermore, a circular concavities  22   c  are respectively provided at opposed positions across the through holes  22   b  of the concave part  22   a . The terminals  24   c  and  25   c  of the electrodes  4  and  5  are inserted in the two concavities  22   c . The terminals  24   c  and  25   c  contact with the two terminals  12   b  and  12   c  on the analyzing unit  6  side connected to the switching circuit  12  when the extraction cartridge  2  is fixed on the analyzing unit  6 , such that the electrodes  4  and  5  are electrically connected to the constant current power supplier  13 . 
         [0040]    Silver/silver chloride electrodes are used for the electrode  4  and electrode  5 . 
         [0041]    The masking tape  27  is provided to prevent direct contact of the skin and the electrodes since the current is known to be painful to the skin. Therefore, the masking tape  27  is an insulating member. As shown in  FIG. 6 , the masking tape  27  contacts with the bottom surfaces of the electrode  4  and electrode  5 . Furthermore, an opening  27   a  is provided in the masking tape  27 , such that tissue fluid can be extracted from the extraction site of the skin to the extraction medium held by the mesh sheet  23  via the opening  27   a.    
         [0042]    The mesh sheet  23  is provided to hold the extraction medium for extracting tissue fluid. The mesh sheet  23  used in the present embodiment is formed of nylon, approximately 10 mm in length, approximately 4 mm in width, and approximately 50 μm in thickness. Purified water is used as the extraction medium, and the mesh sheet  23  is supplied with approximately 1.5 μm of purified water before measurement begins in the blood glucose level measuring device  1 . The purified water used in the present embodiment has an electrical resistivity of 18.3 MΩ, and is essentially an insulator (electrically non-conductive substance). 
         [0043]    The mesh sheet  23  essentially lacks elasticity, and the thickness is substantially unchanged. Furthermore, the mesh sheet  23  is formed of braided nylon fiber approximately 30 μm in thickness, and is a square woven form, 33 μm by 33 μm. As shown in  FIG. 6 , the mesh sheet  23  contacts with the top surface of electrode  4  and electrode  5 , and contacts with the bottom surface of the glucose sensor  70 . 
         [0044]    The glucose sensor  70  is provided to detect the glucose in the tissue fluid extracted to the extraction medium held by the mesh sheet  23 . As shown in  FIG. 11 , the bottom surface of the glucose sensor  70  has a measuring surface  70   a . On the measuring surface  70   a  is applied a color-producing agent to produces a color in reaction with active oxygen, an enzyme (peroxidase) as a catalyst for hydrogen peroxide, and an enzyme (glucose oxidase) as a catalyst for glucose. Furthermore, the glucose sensor  70  is configured by a glass substrate  71 , first optical waveguide layer  72  mounted below the substrate  71 , second optical waveguide layer  73  mounted below the first optical waveguide layer  72 , protective layer  74  formed below the first optical waveguide layer  72  so as to sandwich the second optical waveguide layer  73  between, and light shield layer  75  covering the exterior side of the protective layer  74 . The first optical waveguide layer  72  has a higher refractive index than the substrate  71 . The second optical waveguide layer  73  has a trapezoidal shape inclined to the side, and has a higher refractive index than the first optical waveguide layer  72 . The measurement layer  70   a  of the glucose sensor  70  is an exposed area from the protective layer  74  of the second optical waveguide layer  73 , and contacts with the surface of the mesh sheet  23 . 
         [0045]    The structure of the extraction cartridge set  200  that houses the unused extraction cartridge  2  installed in the blood glucose level measuring device  1  is described below. The extraction cartridge set  200  is configured so as to house the unused extraction cartridge  2  installed in the blood glucose level measuring device  1  in a dry condition, and be capable of including a predetermined amount of purified water (approximately 1.5 μl in the present embodiment) for the extraction cartridge  2  when installed in the blood glucose level measuring device  1 . The extraction cartridge set  200  is provided with a support member  40 , the previously mentioned extraction cartridge  2 , desiccant  50 , liquid supplying member  60 , and separating member  80 , as shown in  FIG. 8 . 
         [0046]    The support member  40  is sheet-like and flexible. The support member  40  is also curved in a U-shape, as shown in  FIG. 7 . As shown in  FIG. 9 , the support member  40  is configured by a cartridge support  41 , liquid supplying member support  42  disposed opposite the cartridge support  41 , and curved part  43  that links to the cartridge support  41  and the liquid supplying member support  42 . Since the support member  40  is essentially shaped as a wallet, it is capable of holding (housing) the extraction cartridge  2  and the liquid supplying member  60  within the region circumscribed by the cartridge support  41 , the liquid supplying member support  42  and the curved part  43 . 
         [0047]    The extraction cartridge  2  is installed so as to be easily removable in the interior side of the cartridge support  41  of the support member  40 . 
         [0048]    The desiccant  50  is provided to inhibit the mesh sheet  23  of the extraction cartridge  2  from absorbing atmospheric moisture and becoming wet. 
         [0049]    The liquid supplying member  60  is a non-woven cloth of absorbent cotton (cut cotton) measuring approximately 15 mm in length, approximately 15 mm in width, and approximately 50 μm in thickness. The liquid supplying member  60  absorbs and holds a predetermined amount (150 μl in the present embodiment) of purified water. As shown in  FIG. 8 , the liquid supplying member  60  is fixedly attached top the interior surface of the liquid supplying member support  42  of the support member  40  so as to confront the mesh sheet  23  of the extraction cartridge  2  mounted on the cartridge support member  41  of the support member  40 . In the present embodiment, approximately 1% of the purified water (approximately 1.5 μl) from the purified water (approximately 150 μl) absorbed by the liquid supplying member  60  is supplied from the liquid supplying member  60  to the mesh sheet  23  of the extraction cartridge  2 . 
         [0050]    The separating member  80  is sheet-like and flexible, similar to the support member  40  described above. The separating member  80  is housed in a region circumscribed by the cartridge support  41  of the U-shaped support member  40 , liquid supplying member support  42 , and curved part  43 , as shown in  FIG. 8 . The separating member  80  is provided with a grip portion  83 , which is provided to be gripped by a subject when the separating member  80  is removed from the support member  40 . The cartridge housing part  81  and liquid supplying member housing part  82  can be gradually peeled from the cartridge support  41  and liquid supplying member support  42  of the support member  40  by gripping and pulling the grip portion  83  in the arrow C direction. 
         [0051]    The subject preparation sequence for measurement by the blood glucose level measuring device  1  is described below using  FIG. 12 . 
       [Preparation Sequence] 
       [0052]    The subject first mounts the band  19  on the wrist (step S 1 ). At this time the band  19  is installed so that the extraction site A on the skin of the subject (refer to  FIG. 6 ) is positioned within the opening  20   a  of the fixture  20  of the band  19  (refer to  FIG. 2 ). Since the electrode  3  is provided on the back surface of the band  19 , the electrode  3  contacts with the surface of the skin B (refer to  FIG. 6 ) when the band is installed on the wrist. 
         [0053]    The subject then performs preprocessing of the extraction site A (refer to  FIG. 6 ) on the wrist of the subject (step S 2 ). Specifically, the subject pierces the extraction site A using the micro needle array  51  shown in  FIG. 10 . Forty-nine micro needles  52  having a length of 0.4 mm and thickness of 0.24 mm protrude at equal spacing within a 10×10 mm surface area on the leading end surface of the micro needle array  51 . By means of the preprocessing, micro pores are formed in the epidermis at the extraction site A, such that tissue fluid can be readily extracted transdermally at the extraction site A. In the present embodiment, the extraction site A on the wrist of the subject is pierced through the opening  20   a  of the fixture  20  of the band  19  so as to accurately match the measuring location and the piercing location. A plurality of extraction holes  121  formed at the extraction site A in this step extend through the corneum layer and granulosum layer and reach near the middle of the corium, but do not reach the subcutaneous tissue. The extraction holes  121  are formed such that their diameter is greater at the skin surface and the diameter is smaller near the subcutaneous tissue. When the extraction holes  121  are formed, tissue fluid filling the corium spreads into the extraction holes  121 , as indicated by the arrow S. Glucose is present in this tissue fluid. 
         [0054]    The subject supplies purified water to the mesh sheet  23  in conjunction with the removal of the unused extraction cartridge  2  from the extraction cartridge set  200  (step S 3 ). Specifically, the subject draws the grip portion  83  of the separating member  80  of the extraction cartridge set  200  in the arrow C direction as shown in  FIG. 7 . Thus, the separating member  80  is pulled from between the dry extraction cartridge  2  and the liquid supplying member  60  that contains purified water, and the cartridge support  41  and liquid supplying member support  42  of the support  40  are pushed from the arrow A direction and arrow B direction by the finger of the subject. In this way the liquid supplying member  60  which contains purified water comes into contact with the dry extraction cartridge  2 , and approximately 1.5 μl of purified water held by the liquid supplying member  60  is supplied to the mesh sheet  23 . 
         [0055]    Next, the subject fixes the extraction cartridge  2  that contains the mesh sheet  23  that is saturated with a predetermined amount (approximately 1.5 μl) of purified water to the analyzing unit  6  by engaging the two connector hooks  17  of the analyzing unit  6  to the pair of mounting holes  21  of the extraction cartridge  2  (step S 4 ). 
         [0056]    Thereafter, the subject installs the analyzing unit  6  with the fixed extraction cartridge  2  on the fixture  20  of the band  19  (step S 5 ). In this way the blood glucose level measuring device  1  is mounted on the wrist of the subject with the purified water-saturated mesh sheet  23  in a state of contact with the skin extraction site A. Thus, the purified water flows from the mesh sheet  23  that is in contact with the extraction site A into the extraction holes  121 , as shown in  FIG. 15 . When the purified water flows into the extraction holes  121 , the tissue fluid that seeped into the extraction holes  121  due to the formation of the extraction holes  121  in step S 1  migrates in the direction of the mesh sheet  23  (the T direction in  FIG. 16 ) as shown in  FIG. 16 . Then, tissue fluid again flows from the corium to the extraction holes  121  because the osmotic pressure within the extraction hole  121  is lower than the osmotic pressure of the corium of the skin. 
         [0057]    After this preparation, the subject measures the blood glucose level via the blood glucose level measuring device  1 . Specifically, the subject operates the input part  14  to input a measurement start command to the controller-analyzer  11  of the blood glucose level measuring device  1 , and the controller-analyzer  11  starts the measuring process.  FIG. 13  is a flow chart showing the sequence of the measuring process performed by the controller-analyzer  11  of the blood glucose level measuring device  1 . Each step of this process is described using the flow chart of  FIG. 13 . 
       Measuring Sequence of the Blood glucose Level Measuring Device  1   
       [0058]    When the measuring start command from the input part  14  is input to the controller-analyzer  11 , the controller-analyzer  11  starts the extraction process. Specifically, the controller-analyzer  11  controls the switching circuit  12  so as to electrically connect the electrode  4  and electrode  5  positioned in the vicinity of the extraction site A to the negative pole of the constant current power supplier  13 , and connect the electrode  3  in contact with the skin surface B to the positive pole of the constant current power supplier  13 , respectively.  FIG. 17  shows the electrical circuit that results from the control performed in this step, and in this condition an electric field is applied to the skin of the subject. 
         [0059]    Then the controller-analyzer  11  controls the constant current power supplier  13  such that a direct current of 150 μA is applied for a predetermined time T (60 seconds in the present embodiment) between the electrode  3 , electrode  4 , and electrode  5 . 
         [0060]    Since the tissue fluid that has seeped into the extraction holes  121  carries an electrical charge, the migration of the tissue fluid is accelerated toward the mesh sheet  23  (T direction in  FIG. 18 ) by imparting an electrical field using the constant current power supplier  13 , as shown in  FIG. 18 . Although the glucose contained in the tissue fluid does not carry an electrical charge, the glucose migrates in conjunction with the migration of other components that do carry an electrical charge. The glucose that has moved to the extraction medium within the mesh sheet  23  is diffused (so-called passive diffusion) within the extraction medium, and arrives at the glucose sensor  70 . The glucose that reaches the measuring surface  70   a  reacts with the glucose oxidase catalyst, and the hydrogen peroxide that is generated as a result then reacts with the peroxidase catalyst. As a result, active oxygen is generated. The color-producing agent painted on the measuring surface  70   a  reacts with the active oxygen and a color is produced. Therefore, the color-producing agent produces a color at an intensity that corresponds to the amount of glucose extracted from the body. 
         [0061]    Light, which is totally reflected within the second optical waveguide layer  73  of the glucose sensor  70  in contact with the mesh sheet  23  saturated with purified water, is absorbed by the color-producing agent in accordance with the amount of glucose extracted from the body, and thereafter arrives at the photoreceptor  34 . As a result, the light, with intensity that is commensurate with the amount of glucose which has arrived at the measuring surface  70   a , impinges the photoreceptor  34 , and a signal corresponding to the intensity of the impinging light is output from the photoreceptor  34 . 
         [0062]    After a predetermined time has elapsed from the application of a current to the skin (60 seconds in the present embodiment), the controller-analyzer  11  obtains an extracted glucose amount (Q) based on the signals output from the photoreceptor  34  (step S 63 ). 
         [0063]    Subsequent to obtaining the extracted glucose amount (Q), the controller-analyzer  11  obtains the electrical conductance of the extraction medium. Specifically, the controller-analyzer  11  controls the switching circuit  12  so as to connect one of the electrodes  4  and  5  to the positive pole of the constant current power supplier  13 , and connect the other electrode to the negative pole of the constant current power supplier  13  (step S 64 ). In the present embodiment, an inverter  12   a  is provided in the switching circuit  12 , so as to have a 50 Hz alternating current flow between the electrode  4  and electrode  5 .  FIG. 19  shows the electrical circuit that results from the control performed in step  64 , and in this condition the electrical conductance of the extraction medium is measured. 
         [0064]    Then, the controller-analyzer  11  controls the constant current power supplier  13  so that a constant current of 50 μA is applied for 5 seconds between the electrode  4  and electrode  5 , and controls the voltmeter  16  so as to measure the voltage level (X) at this time. 
         [0065]    Thereafter, the controller-analyzer  11  calculates the electrical resistance value (R) of the extraction medium as R=X/I by dividing the voltage level (X) by the current value (I) (50 μA in the present embodiment), and obtains the electrical conductance (K) of the extraction medium as K=1/R=I/X (step S 66 ). The electrical conductance of the extraction medium is calculated as the reciprocal of the electrical resistance value (R). 
         [0066]    Then, the controller-analyzer  11  calculates the glucose extraction speed (J) indicating the amount of glucose extracted per unit time (1 second in the present embodiment) (step S 67 ). The glucose extraction speed (J) is calculated as J=Q/T by dividing the extracted glucose amount (Q) obtained in step S 63  by the predetermined time T during which the constant current regulation power supplier  13  applied a current (60 seconds in the present embodiment). 
         [0067]    Then the controller-analyzer  11  calculates the blood glucose level using the equation (1) below based on the electrical conductance (K) of the extraction medium obtained in step S 66 , and the glucose extraction speed (J) obtained in step S 67  (step S 68 ). Although the value calculated using equation (1) below is the glucose concentration in the tissue fluid of the body, the calculation result of equation (1) is used as the blood glucose level in the present embodiment since the glucose concentration of the tissue fluid and the glucose concentration of the blood (that is, the blood glucose level) are virtually equal. 
         [0000]    
       
      
       BG=J/P  
      
     
         [0000]      = J /( ak+b )  (1) 
         [0068]    In equation (1), “BG” represents the calculated blood glucose level, “J” represents the glucose extraction speed which is the amount of glucose extracted per unit time, “P” represents the glucose permeability (ease of glucose pass-through) at the extraction site A, and “K” represents the electrical conductance calculated in step S 63 . Furthermore, “a” and “b” represent preset constants. Equation (1) is stored in the controller-analyzer  11  beforehand and is read therefrom to calculate the blood glucose level each time the blood glucose level is measured. The principle for the blood glucose level calculation used in the present embodiment is described below. 
         [0069]    The controller-analyzer  11  performs control so as to display the blood glucose level calculated in step S 68  on the display  15  (step S 69 ). By displaying the blood glucose level on the display  15 , the subject can thus know his own blood glucose level without the need to collect blood. 
         [0070]    [Principle of the Calculation of the Blood Glucose Level] 
         [0071]    The principle for the blood glucose level calculation used in the present embodiment is described below. 
         [0072]    When a blood glucose level is calculated from the amount of extracted glucose as in the present embodiment, correcting the extracted glucose amount by the glucose permeability (P) of the skin provides a more precise blood glucose level calculation because skin conditions differ by subject and the extracted glucose amount changes depending on the condition of the skin. For example, when the skin is pierced using the same micro needle array  51 , the amount of extracted glucose may increase if the subject has a thin and soft corneum layer because the micro pores are easily formed. On the other hand, the amount of extracted glucose may decrease when the subject has a hard and thick corneum layer because the micro pores are more difficult to be formed. Therefore, in the present embodiment, the glucose permeability (P) at the extraction site A is estimated, and the blood glucose level is calculated by the equation (BG=J/P). 
         [0073]    The electrolyte concentration in the tissue fluid is known to be substantially the same among a plurality of subjects with different blood glucose level. Therefore, it is possible to estimate the degree of the tissue fluid permeation through the skin (that is, the glucose permeability (P)) by measuring the electrolyte content contained in the tissue fluid extracted transdermally. In the present embodiment, purified water that does not contain electrolyte is used as an extraction medium for holding the extracted tissue fluid, and the amount of electrolyte contained in the extracted tissue fluid is estimated by measuring the electrical conductance (K) of the extraction medium when electric power is supplied to the extraction medium containing the extracted tissue fluid. That is, the glucose permeability (P) is estimated from the electrical conductance (K) of the extraction medium containing the extracted tissue fluid. 
         [0074]    Equation (1) is determined by the following experiment. First, a plurality of subjects with a blood glucose level (t) (a constant) (blood glucose level: 80 in the present embodiment) are repeatedly subjected to identical operations of steps S 1  to S 5 , and Step S 6  to S 66 . In this way a plurality of glucose extraction speeds (J) and electrical conductances (K) of the extraction medium are obtained. 
         [0075]    Then, a plurality of coordinate data (J,K), which are derived from the obtained glucose extraction speed (J) and electrical conductance (K) of the extraction medium, are plotted with the glucose extraction speed (J) on the vertical axis and the electrical conductance (K) of the extraction medium on the horizontal axis. The characteristics chart shown in  FIG. 20  is thus obtained. It can be understood from the characteristics chart that the plotted glucose extraction speed (J) and electrical conductance (K) of the extraction medium have a proportional relationship. Thereafter, a linear regression L which represents the relationship between the plotted glucose extraction speed (J) and electrical conductance (K) of the extraction medium, is drawn on the characteristics chart, and the equation (J=αK+β) which represents the line L is determined. 
         [0076]    The relation (J=αK+β) which represents the line L is thus established between glucose extraction speed (J) and electrical conductance (K) of the extraction medium obtained from subjects who have a blood glucose level (t) (constant) (blood glucose level: 80 in the present embodiment). The expression “t=(αK+β)/P” is obtained by substituting “αK+P” for “J” and “t” for “BG” in the expression (BG=J/P) which is a premise of equation (1). 
         [0077]    The glucose permeability (P) can thus be determined as P=(αK+β)/t from the above expression. 
         [0078]    Then, BG=tJ/(αK+β)=J/(αK+b) (where a=a/t, and b=p/t) of equation (1) is obtained by substituting “(αK+β)/t” for “P” in the expression (BG=J/P) which is a premise of equation (1). 
         [0079]    Therefore, discomfort of the subject can be mitigated since the present invention provides an analyzer (measuring device) and analyzing method (measuring method) for obtaining the concentration of predetermined components contained in the tissue fluid in the body without collecting blood. 
         [0080]    Although the tissue fluid is extracted by a method in which a current flows to the extraction site in the present embodiment, tissue fluid may also be extracted without a current flow (so-called passive diffusion extraction). 
         [0081]    Although the tissue fluid is extracted after preprocessing using the micro needle array  51  in the present embodiment, the blood glucose level measuring device  1  may performs the measurements without preprocessing. 
         [0082]    Although the glucose extraction speed is used to determine the blood glucose level in the present embodiment, the glucose concentration in the extraction medium held by the mesh sheet  23  may also be used. 
         [0083]    Although the glucose concentration in the tissue fluid of the body of the subject is considered as the blood glucose level in the present embodiment, a correction may also be performed to convert the glucose concentration in the tissue fluid of the body of the subject to a blood glucose level. 
         [0084]    Although the mesh sheet  23  is dry before the measurement in the present embodiment, a predetermined amount of purified water may be held by the mesh sheet  23  beforehand. In this case, if the purified water held in the mesh sheet  23  has evaporated, a predetermined amount of purified water can be held by the mesh sheet  23  by supplying purified water to the mesh sheet  23  when measurement starts. 
         [0085]    Although the extraction cartridge  2  is installed in the analyzing unit  6  after purified water has been supplied to the mesh sheet  23  provided in the extraction cartridge  2  in the present embodiment, purified water may also be supplied to the mesh sheet  23  after the extraction cartridge  2  is installed in the analyzing unit  6 . This configuration can reduce a deterioration on the glucose sensor  70  caused by contact with the purified water and the extraction cartridge  2 . 
         [0086]    In the present embodiment, the distance between the skin and the glucose sensor during measurement can be uniformly maintained with ease by using a mesh sheet  23  formed of nylon, because the thickness of the mesh sheet  23  doesn&#39;t change when the extraction cartridge  2  is pressed against the skin of the subject. Therefore, measurement errors are reduced. 
         [0087]    Although the extraction cartridge  2  is provided with a mesh sheet  23  in the extraction medium holder formed in a space between the masking tape  27  and the glucose sensor  70  in the present embodiment, the extraction medium holder may also be provided with an absorbent member such as a piece of paper for absorbing and retaining a fluid as a member for holding the extraction medium fluid. Moreover, the extraction medium holder need not be provided with a member such as the mesh sheet  23  and the like, if a space is provided to hold the extraction medium. An electrically non-conductive gel may also be used as the extraction medium held in the space. 
         [0088]    Although purified water is used as the extraction medium supplied to the mesh sheet  23  in the present embodiment, an electrically non-conductive fluid other than purified water may be supplied to the mesh sheet  23 . 
         [0089]    Although the glucose permeability is estimated by extracting tissue fluid into purified water that does not contain electrolyte and by measuring the electrical conductance of the extraction medium containing the extracted tissue fluid in the present embodiment, glucose permeability may also be estimated by extracting tissue fluid to an electrolytic solution such as physiological saline or the like. Note that use of an electrically non-conductive extraction medium is desirable to estimate the glucose permeability with greater precision. 
         [0090]    Although a direct current flow to the skin is provided using a DC constant current power supplier  13  in the present embodiment, a direct current flow to the skin may also be provided using an AC constant current power supplier by providing in the circuit a converter for converting the alternating current to a direct current. 
         [0091]    Although glucose is transdermally extracted by applying a constant current from a constant current power supplier to the skin of a subject and the amount of extracted glucose is corrected using the electrical conductance of the extraction medium in the present embodiment, glucose may also be extracted transdermally by applying a constant voltage from a constant voltage power supplier. Note that, when extracting glucose using an application of a constant voltage, the magnitude of the current flowing to the skin changes according to the electrical resistance of the skin. Since the amount of extracted glucose is dependent on the magnitude of the current flowing to the skin, increasing the current value will increase the amount of extracted glucose. Therefore, when glucose is extracted using a constant voltage, a current measuring section may be provided to monitor the change in the current value during glucose extraction and the amount of extracted glucose may be corrected based on the current value monitored by the current measuring section, in addition to the correction of the extracted glucose amount using the electrical conductance of the extraction medium. For example, the amount of extracted glucose may also be corrected by monitoring the change in the current value during glucose extraction, calculating an average current value, and determining a ratio between the average current value and a standard current value, in addition to the correction using the electrical conductance of the extraction medium. 
         [0092]    Although the electrical conductance of the extraction medium is measured when electric power is supplied to the extraction medium holding tissue fluid extracted transdermally in order to estimate glucose permeability in the present embodiment, the electrical resistance value of the extraction medium when electric power is supplied to the extraction medium, the voltage level applied to the extraction medium, and the current value applied to the extraction medium may be measured. It is also possible to estimate glucose permeability based on the magnitude of the electrical resistance, voltage, and current. 
         [0093]    Although electrical information such as the electrical conductance of the extraction medium is obtained to estimate glucose permeability in the present embodiment, information may also be obtained that reflects the degree of glucose permeation through the skin by measuring the amount of ions present in the tissue fluid extracted transdermally using ion electrodes, insofar as the information obtained reflects the skin permeability of glucose. 
         [0094]    Although silver/silver chloride electrodes are used as the electrode  4  and electrode  5  in the present embodiment, activated carbon electrodes may also be used. When activated carbon electrodes are used, the correlation between the glucose extraction speed and the electrical conductance of the extraction medium can be obtained with greater precision. 
         [0095]    In the present embodiment, the electrodes  4  and  5  are used both when a current flows to the extraction medium and when an electric field is applied to the skin of the subject. This configuration reduces the number of electrodes used in the blood glucose level measuring device  1  and renders the device more compact. In the present embodiment, a switching circuit  12  is provided to switch the output destination of the constant current power supplier  13 . Thus, the number of power suppliers can be reduced, and the device can be rendered even more compact. 
         [0096]    Although the electrodes  4  and  5  are used both when a current flows to the extraction medium and when an electric field is applied to the skin of the subject in the current embodiment, an electrode other than the electrodes  3 ,  4 , and  5  may also be provided independently such that the electrodes  4  and  5  are used when a current flows to the extraction medium, and a current flows between the electrode  3  and the independently provided electrode when an electric field is applied to the skin of the subject. 
         [0097]    Although the blood glucose level of the subject is displayed on the display  15  in the present embodiment, electrical information obtained by supplying electrical power to the extraction medium, such as the electrical conductance and electrical resistance value of the extraction medium, also may be displayed on the display  15 . Accordingly, the subject easily obtains information reflecting the glucose permeability of the skin. 
         [0098]    Although a blood glucose level measuring device  1  for measuring a blood glucose level has been described as an embodiment of the analyzer in the present embodiment, the embodiment is also applicable to analyzers that obtain the concentrations of components in the tissue fluid in the body of the subject based on analysis values of the components contained in the tissue fluid extracted from the body of the subject. The analyzer of the present invention may also measure the concentration of analyzable components such as protein as a biochemical component, the concentrations of drugs administered to the subject and the like. These components may be extracted into the extraction medium in the same manner as in the present embodiment.