Abstract:
Broadly this invention provides inbred corn line ZS01250. The methods for producing a corn plant by crossing the inbred line ZS01250 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01250 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01250 with at least one other corn line.

Description:
FIELD OF THE INVENTION 
     This invention is in the field of corn breeding, specifically relating to an inbred corn line designated ZS01250. 
     BACKGROUND OF THE INVENTION 
     The original maize plant was indigenous to the Western Hemisphere. The plants were weedlike and only through the efforts of early breeders was a cultivated crop species developed. The physical traits of maize are such that self pollination or cross pollination can occur. Each plant has a separate male and female flower, the tassel and ear, respectively. Natural pollination occurs when wind transfers pollen from tassel to the silks on the corn ears. This type of pollination contributed to the wide variation of maize varieties present in the Western Hemisphere. 
     The development of a planned breeding program for maize only occurred in the last century. Originally, maize was an open pollinated variety having heterogeneous genotypes. The maize farmer selected uniform ears from the yield of these genotypes and reserved them for planting the next season. The result was a field of maize plants that were segregating for a variety of traits. This type of maize selection lead to at most incremental increases in seed yield. 
     Large increases in seed yield were the result of the development of hybrid corn varieties in planned breeding programs. Hybrids were developed by selecting corn lines and selfing these lines for several generations to develop homozygous pure inbred lines and crossing selected inbred lines with unrelated inbred lines to produce hybrid progeny (F1). Inbred lines can be difficult to produce since the inbreeding process in corn decreases the vigor. However, when two inbred lines are crossed, the hybrid plant evidences greatly increased vigor compared to open pollinated segregating maize plants. An important factor of the homozygosity and the homogeneity of the inbred lines is that the hybrid from any cross will always be the same, and can be reproduced. 
     The ultimate objective of the commercial maize seed companies is to produce high yielding, agronomically sound plants which perform well in certain regions or areas of the Corn Belt. To produce these types of hybrids, the companies must develop inbreds which carry needed traits into the hybrid combination. Hybrids are not uniformly adapted for the Corn Belt, but are specifically adapted for regions of the Corn Belt. Northern regions of the Corn Belt require shorter season hybrids than do southern regions of the Corn Belt. Hybrids that grow well in Colorado and Nebraska soils may not flourish in rich Illinois soil. Thus, a variety of major agronomic traits are important in hybrid combination for the various Corn Belt regions, and have an impact on hybrid performance. 
     Inbred line development and hybrid testing have been emphasized in the past half century in commercial maize production as a means to increase hybrid performance. Inbred development is usually done by pedigree selection. Pedigree selection can be selection in an F 2  population produced from a planned cross of two genotypes (often elite inbred lines), or selection of progeny of synthetic varieties, open pollinated, composite, or backcross populations. This type of selection is effective for highly inheritable traits, but other traits, for example, yield requires replicated test crosses at a variety of stages for accurate selection. 
     Maize breeders select for a variety of traits in inbreds that impact hybrid performance along with selecting for acceptable parental traits. Such traits include yield potential in hybrid combination; dry down; maturity; grain moisture at harvest; greensnap; resistance to root lodging; resistance to stalk lodging; grain quality; disease and insect resistance; ear and plant height; performance in different soil types such as: low level of organic matter, clay, sand, black, high pH, low pH; performance in: wet environments, drought environments, and no tillage conditions. These traits appear to be governed by a complex genetic system that makes selection and breeding of an inbred line extremely difficult. Even if an inbred in hybrid combination has excellent yield (a desired characteristic), it may not be useful because it fails to have acceptable parental traits such as seed yield, seed size, pollen production, good silks, plant height, etc. 
     To illustrate the difficulty of breeding and developing inbred lines, the following example is given. Two inbreds compared for similarity of 29 traits differed significantly for 18 traits between the two lines. If 18 simply inherited single gene traits were polymorphic with gene frequencies of 0.5 in the parental lines, and assuming independent segregation (as would essentially be the case if each trait resided on a different chromosome arm), then the specific combination of these traits as embodied in an inbred would only be expected to become fixed at a rate of one in 262,144 possible homozygous genetic combinations. Selection of the specific inbred combination is also influenced by the specific selection environment on many of these 18 traits which makes the probability of obtaining this one inbred even more remote. Thus, the general procedure of producing a non segregating F 1  generation and self pollinating to produce a F 2  generation that segregates for traits does not easily lead to a useful inbred. Great care and breeder expertise must be used in selection of breeding material to continue to increase yield and agronomics of inbreds and resultant commercial hybrids. 
     SUMMARY OF THE INVENTION 
     The present invention relates to an inbred corn line ZS01250. Specifically, this invention relates to plants and seeds of this line. Additionally, this relates to a method of producing hybrid seed corn from this inbred. More particularly, this invention relates to the unique combination of traits that combine in corn line ZS01250. 
     Generally then, broadly the present invention includes an inbred corn seed designated ZS01250. This seed produces a corn plant. 
     The invention also includes the tissue culture of regenerable cells of ZS01250 wherein the tissue regenerates plants having the genotype of ZS01250. The tissue culture is selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks and stalks, and cells and protoplasts thereof. The corn plant regenerated from ZS01250 having ZS01250&#39;s genotype. 
     The invention extends to hybrid seed produced by planting, in pollinating proximity, seeds of corn inbred lines ZS01250 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; and harvesting seeds produced on plants of the inbred. The hybrid seed produced by hybrid combination of plants of inbred corn seed designated ZS01250 and plants of another inbred line. Hybrid plants grown from this hybrid seed. 
     The invention further includes a method of hybrid F1 production. A first generation (F1) hybrid corn plant produced by the process of planting, in pollinating proximity, seeds of corn inbred lines ZS01250 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; harvesting seeds produced on plants of the inbred; and growing a harvested seed. 
     A tissue culture of the regenerable cells of hybrid plants produced with use of ZS01250 genetic material. A tissue culture of the regenerable cells of the corn plant produced by the method described above. 
     DEFINITIONS 
     In the description and examples which follow, a number of terms are used. In order to provide a clear and consistent understanding of the specifications and claims, including the scope to be given such terms, the following definitions are provided. 
     BL MOIST 
     The moisture percentage of the grain at black layer, i.e., when 50% of the plants per plot have reached physiological maturity. 
     COLD GERM 
     Cold Germ is a measurement of seed germination under cold soil conditions. Data is reported as percent of seed germinating. 
     ECB 
     European corn borer a maize eating insect. ECBI is the first brood generation of European corn borers. ECBII is the second generation of European corn borers. 
     EMERGE 
     The number of emerged plants per plot (planted at the same seedling rate) collected when plants have two fully developed leaves. 
     GI 
     This is a selection index which provides a single quantitative measure of the worth of a hybrid based on four traits. Yield is the primary trait contributing to index values. The GI value is calculated by combining stalk lodging, root lodging, yield and dropped ears according to the attached mathematical formula: 
     
         GI=100+0.5(YLD)-0.9(%STALK LODGE)-0.9(%ROOT LODGE)-2.7(%DROPPED EAR) 
    
     GLS 
     Gray Leaf Spot (Cercospora Zeae) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
    
     GW 
     Goss&#39; Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
    
     HEATP10 
     The number of Growing Degree Units (GDU&#39;s) or heat units required for an inbred line or hybrid to have approximately 10 percent of the plants shedding pollen. This trait is measured from the time of planting. Growing Degree Units are calculated by the Barger Method where the GDU&#39;s for a 24 hour period are: ##EQU1## 
     The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDU&#39;s to reach various stages of plant development. 
     HEATBL 
     The number of GDU&#39;s after planting when approximately 50 percent of the inbred or hybrid plants in a plot have grain which has reached physiological maturity (black layer). 
     HEATPEEK 
     The number of GDU&#39;s after planting of an inbred when approximately 50 percent of the plants show visible tassel extension. 
     HEATP50 or HTP50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants shedding pollen, Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATP90 
     The number of GDU&#39;s accumulated from planting when the last 100 percent of plants in an inbred or hybrid are still shedding enough viable pollen for pollination to occur. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS10 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 10 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS50 or HTS50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS90 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 90 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     MDMV A   
     Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
    
     MDMV B   
     Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible and a &#34;9&#34; being very resistant.* 
    
     MOISTURE 
     The average percentage grain moisture of an inbred or hybrid at harvest time. 
     NLB 
     Northern Leaf Blight (Exserohilum turcicum) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
    
     PCT TILLER 
     The total number of tillers per plot divided by the total number of plants per plot. 
     PLANT 
     This term includes plant cells, plant protoplasts, plant cell tissue cultures from which corn plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like. 
     PLANT HEIGHT 
     The distance in centimeters from ground level to the base of the tassel peduncle. 
     RM 
     Predicted relative maturity based on the moisture percentage of the grain at harvest. This rating is based on known set of checks and utilizes standard linear regression analyses and is referred to as the Minnesota Relative Maturity Rating System. 
     SHED 
     The volume of pollen shed by the male flower rated on a 1-9 scale where a &#34;1&#34; is a very light pollen shedder, a &#34;4.5&#34; is a moderate shedder, and a &#34;9&#34; is a very heavy shedder. The Table(s) 3 have reduced the 1-9 shed scale to a 1-3 shed scale. Any shed on Table 3 can be multiplied by 3 to reach the 1-9 shed scale. 
     SLB 
     Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
    
     TWT 
     The measure of the weight of grain in pounds for a one bushel volume adjusted for percent grain moisture. 
     VIGOR 
     Visual rating of 1 to 9 made 2-3 weeks post-emergence where a &#34;1&#34; indicates very poor early plant development, and a &#34;9&#34; indicates superior plant development. 
     WARM GERM 
     A measurement of seed germination under ideal (warm, moist) conditions. Data is reported as percent of seeds germinating. 
     YIELD (YLD) 
     Actual yield of grain at harvest adjusted to 15.5% moisture. Measurements are reported in bushels per acre. 
     % DROPPED EARS (DE) 
     The number of plants per plot which dropped their primary ear divided by the total number of plants per plot. 
     % LRG FLAT 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen and a 14/64 inch slot screen, but does not pass through a screen with 20.5/64 inch round openings. 
     % LRG ROUND 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen, but does not pass through a 14/64 inch slot screen or a screen with 20.5/64 inch round openings. 
     % MED FLAT 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen and a 13/64 inch slotted screen, but does not pass through a screen with 17/64 inch round openings. 
     % MEDROUND 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen, but does not pass through a 13/64 inch slot screen or a screen with 17/64 inch round openings. 
     % SML FLAT 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen and a 12/64 inch slotted screen, but does not pass through a screen with 15/64 inch round openings. 
     % SML ROUND 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen, but does not pass through a 12/64 inch slotted screen or a screen with 15/64 inch round openings. 
     % ROOT LODGE (RL) 
     Percentage of plants per plot leaning more that 30 degrees from vertical divided by total plants per plot. 
     % STALK LODGE (SL) 
     Percentage of plants per plot with the stalk broken below the primary ear node divided by the total plants per plot. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     ZS01250 can be used as a male line due to its pollen shed and seed production abilities. This ZS01250 line evidences good general combining ability and specific combining ability with certain types of females. 
     The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. Most of the data in the Variety Description information was collected at Slater, Iowa. 
     The inbred has been self-pollinated for a sufficient number of generations to give inbred uniformity. During plant selection in each generation, the uniformity of plant type was selected to ensure homozygosity and phenotypic stability. The line has been increased in isolated farmland environments with data on uniformity and agronomic traits being observed to assure uniformity and stability. No variant traits have been observed or are expected in ZS01250. 
     The best method of producing the invention, ZS01250 which is substantially homozygous, is by planting the seed of ZS01250 which is substantially homozygous and self-pollinating or sib pollinating the resultant plant in an isolated environment, and harvesting the resultant seed or the resultant pollen. 
     
                       TABLE 1______________________________________ZS01250VARIETY DESCRIPTION INFORMATION#1     Type: Dent#2     Region Best Adapted: North and Canada, 85 GRM.INBRED SE:ZS0125______________________________________#3 MATURITYDAYS  HEATUNITS71    1362       FROM PLANTING TO 50% OF PLANTS IN            SILK70    1295       FROM PLANTING TO 50% OF PLANTS IN            POLLEN 5               FROM 10% TO 90% POLLEN SHED.#4 PLANTDATA2            ANTHOCYANIN OF BRACE ROOTS:        1 = ABSENT 2 = FAINT 3 = MODERATE        4 = DARK#5 LEAFCOLOR/DATA2/MEDIUM GREEN        LEAF COLOR **MUNSELL CODE-5GY 4/44            LEAF SHEATH PUBESCENCE (1 = NONE        TO 9 = PEACH FUZZ)5            MARGINAL WAVES (1 = NONE TO 9 =        MANY)4            LONGITUDINAL CREASES (1 = NONE TO        9 = MANY)#6 TASSELCOLOR/DATA4            POLLEN SHED (0 = STERILE TO 9 =        HEAVY SHEDDER)1/LIGHT GREEN        ANTHER COLOR **MUNSELL        CODE-2.5GY 8/81/LIGHT GREEN        GLUME COLOR **MUNSELL        CODE-5GY 7/82            BAR GLUME: 1 = ABSENT 2 = PRESENT#7A EAR (UNHUSKED DATA)COLOR/DATA1/LIGHT GREEN        SILK COLOR (3 DAYS AFTER EMERGE)        **MUNSELL CODE-2.5GY 8/61/LIGHT GREEN        FRESH HUSK (25 DAYS AFTER 50% SILK)        **MUNSELL CODE-5GY 7/822/TAN       DRY HUSK COLOR (65 DAYS AFTER 50%        SILK **MUNSELL CODE-5Y 8/63            POSITION OF EAR AT DRY HUSK: 1 =        UPRIGHT 2 = HORIZONTAL 3 = PENDENT3            HUSK TIGHTNESS (1 = VERY LOOSE TO        9 = VERY TIGHT)2            HUSK EXTENSION AT HARVEST: 1 =        EXPOSED EAR 2 = 8 CM 3 = 8-10 CM        4 =&gt; 10 CM#7B EAR (HUSKED DATA)DATA2            KERNEL ROWS: 1 = INDISTINCT 2 =        DISTINCT1            ROW ALIGNMENT: 1 = STRAIT 2 =        SLIGHT CURVE 3 = SPIRAL2            EAR TAPPER: 1 = STRAIT 2 =        AVERAGE 3 = EXTREME#8 KERNEL (DRY)COLOR/DATA1            ALEURONE COLOR PATTERN: 1 = HOMO        2 = SEG8/YELLOW-ORNGE        ALEURONE COLOR **MUNSELL        CODE-7.5YR 7/88/YELLOW-ORNGE        HARD ENDOSPERM COLOR        **MUNSELL CODE-7.5YR 6/83            ENDOSPERM TYPE7/YELLOW     CROWN COLOR **MUNSELL CODE-2.5Y        8/10#9 COBCOLOR19/WHITE     COB COLOR **MUNSELL CODE-2.5Y 8/4______________________________________1995 PROJECT SE PVP TRAITSINBRED ZS01250                       T-#10      N     MEAN    STD. STAT  PROB  95% CI______________________________________EAR HEIGHT    15    89.41   7.75 44.67 0.0000                                   (85.48, 93.33)(CM)LENGTH OF    15    72.15   5.23 49.71 0.0000                                   (69.31, 75.00)PRIMARYEAR LEAF(CM)WIDTH OF 15    8.43    1.22 25.80 0.0000                                   (7.79, 9.07)PRIMARYEAR LEAF(CM)TOP EAR  15    6.47    0.52 48.50 0.0000                                   (6.21, 6.73)INTERNODE(CM)DEGREE OF    15    51.00   15.83                       12.47 0.0000                                   (42.99, 59.01)LEAF ANGLE# OF EARS    14    1.13    0.35 12.47 0.0000                                   (0.96, 1.31)PER PLANT# OF LEAVES    13    4.40    0.83 20.58 0.0000                                   (3.98, 4.82)ABOVE TOPEAR# OF     15    6.00    1.07 21.74 0.0000                                   (5.46, 6.54)PRIMARYLATERALTASSELBRANCHESPLANT    15    205.4   9.55 83.34 0.0000                                   (200.6, 210.2)HEIGHT (CM)TASSEL   15    32.87   3.36 37.92 0.0000                                   (31.17, 34.57)LENGTH(CM)TASSEL   15    45.00   0.00             (45.00, 45.00)BRANCHANGLE# OF TILLER    15    0.00    0.00             (0.00, 0.00)PER PLANTSWEIGHT PER    15    25.31   3.26 30.06 0.0000                                   (23.66, 26.96)100 KERNELS(GM)EAR LENGTH    15    16.43   1.61 39.45 0.0000                                   (15.62, 17.25)(CM)EAR WEIGHT    15    86.20   15.51                       21.52 0.0000                                   (78.35, 94.05)(GM)# OF KERNEL    15    10.80   1.01 41.24 0.0000                                   (10.29, 11.31)ROWSCOB DIAM-    15    87.00   3.16 106.6 0.0000                                   (85.40, 88.60)ETER ATMID-POINT(MM)EAR DIAM-    15    122.7   3.72 127.8 0.0000                                   (120.8, 124.5)ETER ATMID-POINT(MM)KERNEL   15    10.20   0.70 56.27 0.0000                                    (9.84, 10.56)LENGTH(MM)KERNEL   15    4.43    0.46 37.51 0.0000                                   (4.20, 4.66)THICKNESS(MM)KERNEL   15    8.43    0.62 52.43 0.0000                                   (8.12, 8.75)WIDTH (MM)% ROUND  15    25.00   0.00             (25.00, 25.00)KERNELS(SHAPEGRADE)SHANK    15    15.43   2.10 28.42 0.0000                                   (14.37, 16.50)LENGTH______________________________________#11 DISEASE RESISTANCE          Northern leaf blight = 1          NLSr2* = 2.5          Gray leaf spot = 2.5          Eye = 1.5          GW = 1          MDMVB = 2#12 Other comparable inbreds are LH160, LH162, and LH164.______________________________________ *Northern Leaf Spot Race Two The Munsell code is a reference book of color which is known and used in the industry and by persons with ordinary skill in the art of plant breeding. The purity and homozygosity of inbred ZS01250 is constantly being tracked using isozyme genotypes as shown in Table 2. 
    
     Isozyme Genotypes for ZS01250 
     Isozyme data were generated for inbred corn line ZS01250 according to procedures known and published in the art. The data in Table 2 gives the electrophoresis data on ZS01250. 
     
                                           TABLE 2__________________________________________________________________________ELECTROPHORESIS RESULTS FOR ZS01250INBREDACP1    ACP4        ADH MDH1                MDH2                    PGD1                        PGD2                            PH1 PGM IDH2__________________________________________________________________________ZS0125033  00  22  22  22  11  11  22  22  11__________________________________________________________________________ 
    
     Inbred and Hybrid Performance of ZS01250 
     The traits and characteristics of inbred corn line ZS01250 are listed to compare with other inbreds and/or in hybrid combination. ZS01250 data shows the characteristics and traits of importance, giving a snapshot of ZS01250. 
     Table 3A shows a comparison between ZS01250 and LH160. ZS01250 is significantly shorter in plant and ear height and has lower seedling emergence and vigor than LH160, ZS01250 sheds pollen and silks at fewer GDU&#39;s than LH160 and form a black layer earlier. Warm and cold germination of ZS01250 is lower than LH160, with seed size being generally smaller. ZS01250 produces significantly less percent large round, large flat and medium round kernel sizes. 
     
                                           TABLE 3A__________________________________________________________________________PAIRED INBRED COMPARISON DATA                     PCT   PLANT EAR          EAR   PCTYEAR  INBRED       VIGOR  EMERGE TILLER                           HEIGHT                                 HEIGHT                                       SHED   QUALITY                                                    BARREN__________________________________________________________________________OVERALL ZS01250       5.0    61.6         134.7 56.7  5.2 LH160 6.0    74.5         153.9 69.1  5.3 # EXPTS       12     12           12    12    10 DIFF  1.0    12.9         19.2  12.3  0.1 PROB  0.026**              0.020**      0.000*                                 0.000*                                       0.885__________________________________________________________________________YEAR  INBRED       HEATP10              HEATP50                     HEATP90                           HEATS10                                 HEATS50                                       HEATS90__________________________________________________________________________OVERALL ZS01250       1209   1267   1392  1242  1292  1338 LH160 1238   1291   1417  1319  1357  1403 # EXPTS       10     10     10    10    10    10 DIFF  30     24     25    76    65    65 PROB  0.032**              0.059***                     0.200 0.000*                                 0.001*                                       0.001*__________________________________________________________________________                     BL    % ROOT                                 % STALK                                       % DROPPEDYEAR  INBRED       HEATPEEK              HEATBL MOIST LODGE LODGE EARS   MOISTURE                                                    YIELD__________________________________________________________________________OVERALL ZS01250       1127   2267   29.0                     10.8  34.3 LH160 1169   2310   29.0                     11.0  56.2 # EXPTS       10     2      1                        12    12 DIFF  43     43     0.0                      0.2   21.9 PROB  0.105  0.077***                        0.239__________________________________________________________________________       WARM   COLD   % LRG % LRG % MED % MED  % SML % SMLYEAR  INBRED       GERM   GERM   ROUND FLAT  ROUND FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01250       83.5   71.1   3.3   3.6   37.8  32.2   11.5  7.7 LH160 91.5   82.0   7.0   6.1   49.9  26.7   6.4   1.9 # EXPTS       11     11     12    12    12    12     12    12 DIFF  8.0    10.8   3.7   2.5   12.1  5.5    5.1   5.8 PROB  0.006* 0.107  0.017**                           0.127 0.000*__________________________________________________________________________ 
    
     Table 3B compares ZS01250 with LH162. ZS01250 is significantly shorter in plant and ear height and has lower seedling emergence and vigor than LH162. ZS01250 sheds less pollen and sheds pollen earlier than LH162, as evidenced by the significantly SHED, HEATP50 and HEATP90 ratings. Warm germination of ZS01250 is lower than LH162. ZS01250 produces fewer large flat kernels and significantly more medium round kernels. 
     
                                           TABLE 3B__________________________________________________________________________PAIRED INBRED COMPARISON DATA                     PCT   PLANT EAR          EAR   PCTYEAR  INBRED       VIGOR  EMERGE TILLER                           HEIGHT                                 HEIGHT                                       SHED   QUALITY                                                    BARREN__________________________________________________________________________OVERALL ZS01250       5.0    62.7         137.5 56.8  5.2 LH160 5.8    88.1         145.5 64.6  7.4 # EXPTS       11     11           11    11    9 DIFF  0.8    25.4         8.0   7.8   2.2 PROB  0.031**              0.000*       0.075***                                 0.037**                                       0.002*__________________________________________________________________________YEAR  INBRED       HEATP10              HEATP50                     HEATP90                           HEATS10                                 HEATS50                                       HEATS90__________________________________________________________________________OVERALL ZS01250       1215   1274   1392  1244  1294  1332 LH160 1244   1305   1434  1262  1292  1335 # EXPTS       9      9      9     9     9     9 DIFF  29     32     41    17    2     3 PROB  0.138  0.024**                     0.015**                           0.325 0.873 0.782__________________________________________________________________________                     BL    % ROOT                                 % STALK                                       % DROPPEDYEAR  INBRED       HEATPEEK              HEATBL MOIST LODGE LODGE EARS   MOISTURE                                                    YIELD__________________________________________________________________________OVERALL ZS01250       1132   2267   29.0                     10.8  35.8 LH160 1143   2255   28.0                     11.4  62.1 # EXPTS       9      2      1                        11    11 DIFF  11     11     1.0                      0.6   26.3 PROB  0.592  0.173                           0.110__________________________________________________________________________       WARM   COLD   % LRG % LRG % MED % MED  % SML % SMLYEAR  INBRED       GERM   GERM   ROUND FLAT  ROUND FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01250       83.8   70.5   3.4   3.7   37.4  32.5   11.3  7.7 LH160 95.9   72.2   4.4   6.2   23.6  35.9   10.2  12.3 # EXPTS       10     10     11    11    11    11     11    11 DIFF  12.0   1.8    1.0   2.6   13.8  3.4    1.1   4.6 PROB  0.000* 0.822  0.555 0.007*                                 0.000*__________________________________________________________________________ 
    
     Table 3C is an inbred comparison between ZS01250 and LH164. ZS01250 plant hybrid is significantly less, but ear height significantly more. Seedling emergence, vigor and amount of pollen shed is significantly less than LH164. ZS01250 is an earlier flowering line as evidenced by the lower GDU&#39;s at HEATPEEK, all HEATP and all HEATS ratings. Grain moisture at harvest is greater. Warm and cold germination of ZS01250 is significantly lower than LH164. Seed size of ZS01250 is smaller with significantly fewer large round and large flat kernels produced than LH164. 
     
                                           TABLE 3C__________________________________________________________________________PAIRED INBRED COMPARISON DATA                     PCT   PLANT EAR          EAR   PCTYEAR  INBRED       VIGOR  EMERGE TILLER                           HEIGHT                                 HEIGHT                                       SHED   QUALITY                                                    BARREN__________________________________________________________________________OVERALL ZS01250       5.0    60.7         132.8 55.2  4.8 LH160 5.4    80.1         145.3 48.3  8.2 # EXPTS       11     11           11    11    9 DIFF  0.5    19.4         12.4  6.9   3.4 PROB  0.219  0.020**      0.008*                                 0.028**                                       0.000*__________________________________________________________________________YEAR  INBRED       HEATP10              HEATP50                     HEATP90                           HEATS10                                 HEATS50                                       HEATS90__________________________________________________________________________OVERALL ZS01250       1210   1270   1400  1247  1298  1346 LH160 1315   1361   1494  1366  1403  1441 # EXPTS       9      9      9     9     9     9 DIFF  105    90     93    118   106   95 PROB  0.001* 0.001* 0.000*                           0.000*                                 0.000*                                       0.000*__________________________________________________________________________                     BL    % ROOT                                 % STALK                                       % DROPPEDYEAR  INBRED       HEATPEEK              HEATBL MOIST LODGE LODGE EARS   MOISTURE                                                    YIELD__________________________________________________________________________OVERALL ZS01250       1124   2267   29.0                     10.7  35.1 LH160 1198   2401   29.5                     9.7   56.6 # EXPTS       9      2      1                        11    11 DIFF  74     134    0.5                      1.0   21.5 PROB  0.012**              0.183                           0.043**__________________________________________________________________________       WARM   COLD   % LRG % LRG % MED % MED  % SML % SMLYEAR  INBRED       GERM   GERM   ROUND FLAT  ROUND FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01250       84.2   71.4   3.2   3.7   39.3  30.7   12.1  7.3 LH160 90.8   82.0   27.7  12.6  39.2  13.7   4.5   1.2 # EXPTS       10     10     9     9     9     9      9     9 DIFF  6.6    10.6   24.5  8.9   0.0   17.1   7.5   6.1 PROB  0.002* 0.053***                     0.000*                           0.000*                                 0.997__________________________________________________________________________ 
    
     Table 4 shows the GCA (general combining ability) estimates of ZS01250 compared with the GCA estimates of the other inbreds. The estimates show the general combining ability is weighted by the number of experiment/location combinations in which the specific hybrid combination occurs. The interpretation of the data for all traits is that a positive comparison is a practical advantage. A negative comparison is a practical disadvantage. The general combining ability of an inbred is clearly evidenced by the results of the general combining ability estimates. This data compares the inbred parent in a number of hybrid combinations to a group of &#34;checks&#34;. The check data is from other companies&#39; hybrids, particularly the leader in the industry and ICI Seeds&#39; commercial products and pre-commercial hybrids which were grown in the same sets and locations. 
     Table 4A shows ZS01250 crossed in 84 hybrid comparisons. ZS01250 shows average Y/M, a positive moisture and stalk lodge advantage and a negative root lodge advantage. 
     
                                           TABLE 4A__________________________________________________________________________ADVANTAGES OVER COMMERCIALS &amp; N&#39;S   N93 N94    N95       N  FI Y M                GI I  YLD                         MST                            % SL                               % RL                                  % DE                                     TWT                                        POP                                           RM__________________________________________________________________________XR =   28 4  53 87 0.8             0.0                -0.6                   0.6                      -0.8                         0.5                            3.1                               -3.3                                  -0.1                                     -0.3                                        -366                                           87XH =        12 0.2             -0.0                0.5                   -1.1                      -1.7                         0.0                            4.7                               -1.8                                  -0.1                                     0.1                                        -332                                           87XT =        2  0.7             -0.2                -0.0                   2.8                      -1.9                         0.3                            2.6                               -2.3                                  0.0                                     -0.2                                        -376                                           91__________________________________________________________________________ XR = GCA ESTIMATE: WEIGHTED BY EXPT XH = GCA ESTIMATE: WEIGHTED BY PARENT2 XT = SAME AS XH, BUT USING ONLY THOSE PARENT2 WITH TWO YEARS OF DATA 
    
     Table 4B compares ZS01250 in random hybrid combinations with LH160, LH162, and LH164 in random combinations. In hybrid combination, ZS01250 has a greater positive advantage for stalk lodging and a larger negative advantage for root lodging compared to all three inbreds. ZS01250 generally has better advantage for Y/M, GI and yield compared to LH160 and LH162. LH162 has the advantage for moisture. Compared to LH164, ZS01250 has less advantage except for the strong stalk lodging advantage. 
     
                       TABLE 4B______________________________________              N    FI   YM   GI   I    YLD   MSTLH160  XR    =     545  -0.7 -0.2 -1.4 -1.5 -3.6  0.3LH162  XR    =     961  0.5  0.1  -3.3 -2.2 -5.8  1.6LH164  XR    =     471  2.5  0.3  0.6  0.4  -0.5  0.8               % SL % RL  % DE  TWT  POP   RMLH160  XR    =      0.8  -0.3  0.0   1.3  -263  93LH162  XR    =      -0.1 -0.2  -0.0  0.3  -133  84LH164  XR    =      0.6  0.4   -0.0  -0.3 -170  89______________________________________ 
    
     Table 5A shows the advantage the ZS01250 hybrid has over a commercially available ICI Seeds&#39; hybrid. The ZS01250 hybrid has only a slight moisture disadvantage compared to 8984, but a significant yield advantage and is 5.6 percent better for stalk lodging. Root lodging for the ZS01250 is a negative advantage, but here is a tendency to fewer dropped ears and better test weight. 
     
                                           TABLE 5A__________________________________________________________________________HYBRID SUMMARYZS01250/INBREDHYBRID  TESTS       GI ADV             FI ADV                   YLD                      ADV                         MOIST                             ADV                                SL__________________________________________________________________________ZS01250RE 4   129          14 78 8  81.2                      8.9                         22.1                             -2.2                                13.18984 RE 4   115   70    72.3  19.9   23.2RE 17  167          7  120                7  145.6                      8.9*                         20.2                             -0.1                                2.0RE 17  160   113   136.7 20.1   6.5RE 12  149          -4 107                -5 124.2                      -2.8                         18.4                             -0.7                                5.1RE 12  153   112   127.0 17.7   10.5RE 33  156          4  110                3  130.0                      4.6*                         19.8                             -0.6                                4.5RE 33  152   107   125.4 19.2   10.1__________________________________________________________________________HYBRID  ADV RL ADV             DE ADV                   TWT                      ADV                         POP ADV__________________________________________________________________________ZS01250RE 10.1       0.0          0.0             0.0                0.0      25101                             -1E38984 RE     0.0   0.0         26136RE 4.5 3.5          -1.4             0.0                0.2                   49.2                      -0.6                         27443                             -313RE     2.2   0.3   49.8  27756RE 5.5 9.0          -7.7             0.2                -0.1                   51.4                      1.5                         25918                             109RE     1.3   0.0   49.9  25809RE 5.6 5.1          -3.6             0.1                0.1                   50.1                      0.2                         26578                             -245RE     1.6   0.1   49.9  26823__________________________________________________________________________ 
    
     Table 7A shows the entomology strengths and weaknesses of the ZS01250 inbred. 
     
                       TABLE 7A______________________________________                       EAR     PLANT   ECB1      ECB2      DAMAGE  INTEGRITYINBRED  RATING    RATING    RATING  RATING______________________________________ZS01250 4.3       5.5       1       8.9______________________________________ 
    
     The inbred ZS01250 can be employed as the male plant in a hybrid production field. This inbred is a medium short line with low ear height. In hybrid combination, ZS01250 makes medium height hybrids with moderately low ear height. ZS01250 hybrids have good yield for maturity and perform well in the northern corn belt and Canada. Stalk strength is excellent for an early line, but there is a tendency to root lodge. 
     The foregoing is set forth by way of example and is not intended to limit the scope of the invention. 
     This invention also is directed to methods for producing a corn plant by crossing a first parent corn plant with a second parent corn plant wherein the first or second parent corn plant is an inbred corn plant from the line ZS01250. Further, both first and second parent corn plants can come from the inbred corn line ZS01250. A variety of breeding methods can be selected depending on the mode of reproduction, the trait, the condition of the germplasm. Thus, any such methods using the inbred corn line ZS01250 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, haploid and anther culturing and the like. 
     Various culturing techniques known to those skilled in the art, such as haploid, transformation, and a host of other conventional and unconventional methods are within the scope of the invention. All plants and plant cells produced using inbred corn line ZS01250 are within the scope of this invention. The invention encompasses the inbred corn line used in crosses with other, different, corn inbreds to produce (F1) corn hybrid seeds and plants with the characteristics that make good hybrids. This invention includes cells which upon growth and differentiation produce corn plants having the physiological and morphological characteristics of the inbred line ZS01250. 
     Duncan, from at least 1985-1988 produced literature on plant regeneration from callus. Both inbred and hybrid callus have resulted in regenerated plants at excellent efficiency rates. Somatic embryogenesis has been performed on various maize tissue such as glume which before the 1980&#39;s was considered unusable for this purpose. The prior art clearly teaches the regeneration of plants from various maize tissues. 
     European Patent Application, publication 160,390, describes tissue culture of corn which can be used by those skilled in the art. Corn tissue culture procedures are also described in the literature as early as 1982. 
     A deposit of at least 2500 seeds of the inbred seed of this invention is maintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Access to this deposit will be available during the pendency of this application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. All restrictions on availability to the public of such material will be removed upon allowance of any claims of this application by depositing at least 2500 seeds of this invention at the American Type Culture Collection, Rockville, Md. The Applicant made a deposit on Jun. 11, 1997, of at least 2500 seeds of Inbred Corn Line ZS01250 with the American Type Culture Collection (ATCC), located at 12301 Parklawn Drive, Rockville, Md. 20852. The ATCC accession number is 209108. Additionally the Applicant has satisfied all of the requirements of C.F.R.§1.801-1.809, including providing an indication of the viability of the sample. The ATCC deposit will be maintained in that depository, which is a public depository, for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. 
     Inbreds designated LH are available from Holden&#39;s Foundation Seed, Inc. in Iowa. Inbreds designated MBS are available from Mike Brayton Seed in Iowa. Inbreds designated SGI are available from Seed Genetic Inc. in New Jersey. Information on some ZS designations may be available from the PVP office. 
     Accordingly, the present invention has been described with some degree of particularity directed to the preferred embodiment of the present invention. It should be appreciated, though, that the present invention is defined by the following claims construed in light of the prior art so that modifications or changes may be made to the preferred embodiment of the present invention without departing from the inventive concepts contained herein.