Abstract:
Boron compound complexing reagents, boron compound complexes, and methods of synthesizing these reagents and complexes are disclosed. These reagents and complexes include those shown in General Formula III, General Formula IV, and General Formula VI. In one embodiment, the reagents of General Formula III may be used to produce, after condensation with a bioactive species (BAS), the reagent of General Formula IV. The reagent of General Formula IV may be used to form a complex with a boron compound, such as a complex shown in General Formula VI. ##STR1##

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a continuation in part of U.S. application Ser. No. 08/188,531 which was filed on Jan. 28, 1994 and is now U.S. Pat. No. 5,594,151 and is hereby incorporated herein by reference. 
    
    
     FIELD OF THE INVENTION 
     The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. 
     This application also follows application Ser. No. 08/188,460, now abandoned, and U.S. Pat. Nos. 5,594,111 and 5,623,055. 
     BACKGROUND OF THE INVENTION 
     Bioconjugation is a descriptive term for the joining of two or more different molecular species by chemical or biological means, in which at least one of the molecular species is a biological macromolecule. This includes, but is not limited to, conjugation of proteins, peptides, polysaccharides, hormones, nucleic acids, liposomes and cells, with each other or with any other molecular species that add useful properties, including, but not limited to, drugs, radionuclides, toxins, haptens, inhibitors, chromophores, fluorophores, ligands, etc. Immobilization of biological macromolecules is also considered a special case of bioconjugation in which the macromolecule is conjugated, either reversibly or irreversibly, to an insoluble support. Bioconjugation is utilized extensively in biochemical, immunochemical and molecuar biological research. Major applications of bioconjugation include; detection of gene probes, enzyme-linked immuno solid-phase assay, monoclonal antibody drug targeting and medical imaging. 
     Bioconjugates are generally classified as either direct or indirect conjugates. Direct conjugates encompass those in which two or more components are joined by direct covalent chemical linkages. Alternatively, indirect conjugates encompass those in which two or more components are joined via an intermediary complex involving a biological macromolecule. The system described herein is the first to enable the formation of indirect conjugates without dependence upon an intermediary biological macromolecule. 
     AVIDIN-BIOTIN SYSTEM 
     Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system, in which, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium Streptomyces avidinii), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. Both Avidin and Streptavidin possess four Biotin binding sites of very high affinity (K=10 15  mol -1 )). 
     The Avidin-Biotin system has been utilized extensively for enzyme-linked immuno solid-phase assay (ELISA), in which an enzyme-Avidin conjugate (useful for detection by reaction with the enzyme&#39;s substrate to afford a colored or chemiluminescent product) is employed to detect the presence of a biotinylated antibody, after first binding the antibody to an immobilized antigen or hapten. Applications of the Avidin-Biotin system number in the hundreds, and have recently been reviewed (Wilchek, M. and Bayer, E. A., (1990) Methods in Enzymology, 184). Although utilized extensively, several limitations are known to be associated with the Avidin-Biotin system, which include nonspecific binding generally attributed to the basicity of the Avidin molecule, nonspecific binding attributed to the presence of carbohydrate residues on the Avidin molecule, and background interference associated with the presence of endogenous Biotin, which is ubiquitous in both eukaryotic and prokaryotic cells. 
     DIGOXIGENIN α-DIGOXIGENIN SYSTEM 
     An alternative indirect bioconjugation system designed to overcome some of the limitations associated with the Avidin-Biotin system has recently been developed for the detection of gene probes by ELISA (Kessler, C., Holtke, H.-J., Seibl, R., Burg, J. and Muhlegger, K., (1990) Biol. Chem. Hoppe-Seyler, 371, 917-965. This system involves the use of the steroid hapten Digoxigenin, an alkaloid occuring exclusively in Digitalis plants, and Fab fragments derived from polyclonal sheep antibodies against Digoxigenin (α-Digoxigenin). The high specificity of the various α-Digoxigenin antibodies affords low backgrounds and eliminates the non-specific binding observed in Avidin-Biotin systems. Digoxigenin-labeled DNA and RNA probes can detect single-copy sequences in human genomic Southern blots. The development of the Digoxigenin α-Digoxigenin system has recently been reviewed (Kessler, C. (1990) in Advances in Mutagenesis Research (Obe, G. ed.) pp. 105-152, Springer-Verlag, Berlin/Heidelberg). The Digoxigenin α-Digoxigenin system is the most recent representative of several hapten-antibody systems now utilized for bioconjugation. 
     IMMOBILIZED PHENYLBORONATES 
     Phenylboronic acids are known to interact with a wide range of polar molecules having certain requisite functionalities. Complexes of varying stability, involving 1,2-diols, 1,3-diols, 1,2-hydroxy acids, 1,3-hydroxy acids, 1,2- hydroxylamines, 1,3-hydroxylamines, 1,2-diketones and 1,3-diketones, are known to form with either neutral phenylboronic acid or phenylboronate anion. Consequently, immobilized phenylboronic acids have been exploited as chromatographic supports to selectively retain, from diverse biological samples, those molecular species having the requisite functionalities. Many important biological molecules including carbohydrates, catecholamines, prostaglandins, ribonucleosides, and steroids contain the requisite functionalities, and have been either analyzed or purified in this manner. The use of phenylboronic acid chromatographic media for the isolation and separation of biological molecules has been discussed in several reviews (Singhal, R. P. and DeSilva, S. S. M. (1992) Adv. Chromatog., 31, 293-335; Mazzeo, J. R. and Krull, I. S. (1989) BioChromatog., 4, 124-130; and Bergold, A. and Scouten, W. H. (1983) in Solid Phase Biochemistry (Scouten, W. H. ed.) pp. 149-187, John Wiley &amp; Sons, New York). 
     Phenylboronic acid, like boric acid, is a Lewis acid, and ionizes not by direct deprotonation, but by hydration to give the tetrahedral phenylboronate anion (pK a  =8.86). Phenylboronic acid is three times as strong an acid as boric acid. Ionization of phenylboronic acid is an important factor in complex formation, in that, upon ionization, boron changes from trigonal coordination (having average bond angles of 120° and average bond lengths of 1.37≈) to the tetrahedrally coordinated anion (having average bond angles of 109° and average bond lengths of 1.48≈). 
     Molecular species having cis or coaxial 1,2-diol and 1,3-diol functionalities, and particularly carbohydrates, are known to complex with immobilized phenylboronate anion, to form cyclic esters under alkaline aqueous conditions (Lorand, J. P. and Edwards, J. O. (1959) J. Org. Chem., 24, 769). 
     Acidification of 1,2-diol and 1,3-diol complexes to neutral pH is know to release the diol containing species, presumably due to hydrolysis of the cyclic ester. Coplanar aromatic 1,3-diols, like 1,8-dihydroxynaphthalene, are known to complex even under acidic conditions due to the hydrolytic stability of six-membered cyclic boronic acid esters (Sienkiewicz, P. A. and Roberts, D. C. (1980) J. Inorg. Nucl. Chem., 42, 1559-1571). Molecular species having pendant 1,2-hydroxylamine, 1,3-hydroxylamine, 1,2-hydroxyamide, 1,3-hydroxyamide, 1,2-hydroxy-oxime and 1,3-hydroxyoxime functionalities are also known to reversibly complex with phenylboronic acid under alkaline aqueous conditions similar to those associated with the retention of diol containing species (Tanner, D. W. and Bruice, T. C. (1967) J. Amer. Chem. Soc., 89, 6954). 
     PHENYLBORONATE BIOCONJUGATES 
     Ortho-substituted acetamidophenylboronic acids have been proposed as potential linkers for selective bioconjugation via the vicinal diol moieties of the carbohydrate residues associated with glycoproteins (Cai, S. X. and Keana, J. F. W. (1991) Bioconjugate Chem., 2, 317-322). 
     Phenylboronic acid bioconjugates derived from 3-isothiocyanatophenylboronic acid have been successfully utilized for appending radioactive technetium dioxime complexes to monoclonal antibodies for use in medical imaging (Linder, K. E., Wen, M. D., Nowotnik, D. P., Malley, M. F., Gougoutas, J. Z., Nunn, A. D. and Eckelman, W. C. (1991) Bioconjugate Chem., 2, 160-170; Linder, K. E., Wen, M. D., Nowotnik, D. P., Ramalingam, K., Sharkey, R. M., Yost, F., Narra, R. K. and Eckelman, W. C. (1991) Bioconjugate Chem., 2, 407-414). 
     3-Aminophenylboronic acid has been covalently appended to proteins by a variety of chemical methods and the resulting phenylboronic acid bioconjugates tested for their binding of D-sorbitol, D-mannose and glycated hemoglobin (GHb). The interactions proved to be reversible and of very low affinity rendering the bioconjugates of very limited practical use. Similarly, an alkaline phosphatase phenylboronic acid bioconjugate used in an attempted enzyme-linked assay for the detection of GHb failed to detect the presence of glycated protein (Frantzen, F., Grimsrud, K., Heggli, D. and Sundrehagen, E. (1995) Journal of Chromatography B, 6 70, 37-45). 
     Although immobilized phenylboronates have been utilized for chromatographic separation of biological molecules having the requisite functionalities, notwithstanding the substantial amount of research into bioconjugation, and the substantial amount of investment in this field, the selectivity of phenylboronic acid has not heretofore been successfully exploited to enable the conjugation of biological macromolecules with one another or with other molecular species that add useful properties. 
     SUMMARY OF THE INVENTION 
     The present invention relates to a novel class of boron compound complexing reagents useful for the preparation of bioconjugates, and the method of making and using such reagents. In one embodiment, the boron compound is phenylboronic acid, or derivatives thereof, which complex with the complexing reagents of the present invention. Unless otherwise noted, the phrase phenylboronic acid complexing reagent is used herein to include the broader class of boron compound complexing reagents, and the phrase phenylboronic acid is used herein to include the broader class of boron compounds which complex with the boron compound complexing reagents. In the present invention, in the place of prior art Avidin-Biotin and Digoxigenin α-Digoxigenin systems, boron compound complexing reagents are utilized in conjunction with the boron compound, such as phenylboronic acid reagents (many of which are known in the prior art) to facilitate chemical conjugation without the use of intermediary biological macromolecules. Bioconjugate preparation often involves the conjugation of several components including, but not limited to, proteins, peptides, polysaccharides, hormones, nucleic acids, liposomes and cells, with each other or with any other molecular species that add useful properties, including, but not limited to, drugs, radionuclides, toxins, haptens, inhibitors, fluorophores, ligands, solid-phase supports, and boron compound complexing reagents conjugates. These various components utilized in bioconjugate preparation will collectively and individually be termed biologically active species or bioactive species. 
     Reagents suitable for the modification of a bioactive species for the purpose of incorporating a phenylboronic acid complexing moiety for subsequent conjugation to a different (or the same) bioactive species having pendant phenylboronic acid moieties are of the general formula of General Formula III: ##STR2## Group R 1  is a reactive electrophilic or nucleophilic moiety suitable for reaction of the phenylboronic acid complexing reagent with a bioactive species. Group Z is a spacer selected from a saturated or unsaturated chain of from about 0 to 6 carbon equivalents in length, an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one of intermediate amide or disulfide moieties, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. Group R 3  is selected from one of an H, an alkyl, and a methylene or ethylene moiety with an electronegative substitutent. 
     Group R 3  is preferably selected from one of H, CH 3 , CH 2  CN, CH 2  COOH, CH 2  CONH 2 , CH 2  CH 2  OH and CH 2  OCH 3 . When group R 3  is H, group R 1  is preferably selected from one of acrylamide, amino, dithiopyridyl, hydrazide, imidate ester, maleimide, and thiol moieties. Group Z is preferably an unbranched alkyl chain of the general formula (CH 2 ) n , wherein n=1 to 6. 
     Reaction of a reagent of General Formula III with a bioactive species affords a conjugate having pendant phenylboronic acid complexing moieties (one or more) of General Formula IV, ##STR3## The symbol labeled BAS represents a biologically active species (or bioactive species) that may or may not contain a portion of a reactive moiety (which may itself have a spacer) used to attach the bioactive species to the reagent. It will be appreciated that, in many embodiments, several identical reagents of the general formula of General Formula III will react with a single BAS molecule. For example, if the BAS is a protein, many phenylboronic acid complexing reagents will react with the protein, each reacting at one of the several sites on the protein which are reactive with the R 1  group. Group Z in General Formula IV is a spacer selected from a saturated or unsaturated chain of from about 0 to 6 carbon equivalents in length, an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one of intermediate amide or disulfide moieties, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. Group R 3  is selected from one of an H, an alkyl, and a methylene or ethylene moiety with an electronegative substitutent. 
     The compositions of General Formulas I and II, and methods for their preparation are described herein and are the subject of my copending application, titled Boronic Compound Complexing Reagents and Complexes. U.S. Ser. No. 08/689,341, and filed on Aug. 5, 1996, by Mark L. Stolowitz, Robert J. Kaiser, Kevin P. Lund and Steven M. Torkelson, which copending application is hereby incorporated herein by reference. 
     Phenylboronic acid reagents, many of which are known in the prior art, as well as those described in greater detail in my copending application, titled Phenylboronic Acid Complexes For Bioconjugate Preparation, and filed on Jan. 28, 1994, Ser. No. 08/188,958, now U.S. Pat. No. 5,594,151, which is incorporated herein by reference, may be appended to a biologially active species to afford a conjugate having pendant phenylboronic acid moieties (one or more) of General Formula V: ##STR4## wherein the symbol labeled BAS* represents a second bioactive species, that may include a linker portion and that may differ from the bioactive species labeled BAS. The BAS* may also include a portion of a reactive moiety used to attach the bioactive species to the phenylboronic acid reagent. 
     A conjugate of General Formula IV, with at least one biologically active species and having pendent phenylboronic acid complexing moities (one or more), may be complexed with a conjugate of General Formula V, prepared from a second bioactive species BAS* and having pendant phenylboronic acid moities (one or more), to afford a bioconjugate of General Formula VI, ##STR5## wherein the symbols labeled BAS and BAS*, and groups Z and R 3  are as were previously defined. In this manner, biological macromolecules may be conjugated to one another or with other functionalities which impart useful properties. 
     In summary, boron compound complexing reagents, boron compound complexes, and method of synthesizing these reagents and complexes are described. These reagents and complexes include those shown in General Formulas III, IV and VI. In one embodiment, the reagents of General Formula III may be used to produce, after condensation with a bioactive species (BAS), the reagent of General Formula IV. The reagent of General Formula IV may be used to form a complex with a boron compound, such as a complex shown in General Formula VI. 
     Bioconjugates of General Formula VI may be prepared in buffered aqueous solution or organic solvents. The bioconjugate is formed within a few minutes over a range of temperatures of from about 4° C. to 70° C. The stability of the bioconjugate in aqueous solution at a given pH and temperature is significantly influenced by substituent group R 3 . Bioconjugates of General Formula VI, are stable in aqueous solutions of approximate pH greater than 3.5 and less than 10.5. The bioconjugation reaction (phenylboronic acid complexation) is insensitive to significant variations in ionic strength, the presence of organic solvents, the presence of detergents, and the presence of chaotropic agents (protein denaturants), which are incompatible with prior art indirect labeling systems wherein the structure of a biological macromolecule must be maintained to preserve requisite binding properties. In most instances, the constraints governing the formation of bioconjugates, by the system herein described, are limited to those imposed by the conditions required to maintain viability of the bioactive species. 
    
    
     BRIEF DESCRIPTION OF THE FIGURES 
     FIG. 1 illustrates the utilization of putative phenylbornic acid complexing reagents of General Formula I and phenylboronic acid complexing reagents of General Formula III, to prepare conjugates of General Formula IV which may, in turn, be utilized to prepare bioconjugates of General Formula VI. 
     FIG. 2 summarizes the preparation of alkyl 4- and 5-aminomethylsalicylates, synthetic intermediates leading to reagents of General Formula I, wherein R 2  is an alkyl group, e.g., methyl, ethyl, propyl, etc. Alkyl 4- and 5-aminomethylsalicylates are also useful synthetic intermediates leading to reagents of General Formula III. 
     FIG. 3 summarizes the preparation of alkyl 4- and 5-aminomethylsalicylates, synthetic intermediates leading to reagents of General Formula I, wherein R 2  is a methylene group bearing an electronegative moiety, e.g., carboxymethyl, cyanomethyl, methoxymethyl, etc. 
     FIG. 4 summarizes the preparation of 4- and 5-aminomethylsalicylhydroxamic acids, synthetic intermediates leading to reagents of General Formula III, wherein R 3  is one of either an alkyl group or a methylene or ethylene group bearing an electronegative moiety. 
     FIG. 5 summarizes the synthesis of reagents of General Formulas I and III, wherein R 2  is one of either an alkyl group or a methylene group bearing an electronegative moiety, and wherein R 1  is selected from either imidazolide, hydrazide and N-hydroxysuccinimidyl ester moieties. 
     FIG. 6 summarizes the synthesis of reagents of General Formulas I and III, wherein R 2  is one of either an alkyl group or a methylene group bearing an electronegative moiety, and wherein R 1  is selected from either bromo, chloro, iodo, maleimide, dithiopyridyl and imidate ester moieties. 
     FIG. 7 summarizes the synthesis of reagents of General Formulas I and III, wherein R 1  is an N-hydroxysuccinimidy ester, and wherein Z an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one of either an intermediate amide or disulfide moiety. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     A two-step process which utilizes reagents of General Formula III for the preparation of bioconjugates is summarized in FIG. 1. Initially, a reagentof General Formula III is selected that is comprised of an appropriate reactive electrophilic or nucleophic group R 1  suitable for reaction with the desired biologically active species. ##STR6## 
     Group R 1  is a reactive electrophilic or nucleophilic moiety suitable for reaction of the phenylboronic acid complexing reagent with a bioactivespecies. Group R 1  is preferably selected from, but not limited to, acrylamide, bromo, dithiopyridyl, bromoacetamide, hydrazide, N-hydroxysuccinimidyl ester, N-hydroxysulfosuccinimidyl ester, imidate ester, imadazolide, iodo, iodoacetamide, maleimide, amino and thiol moieties. 
     Group Z is a spacer selected from a saturated or unsaturated, preferably unbranched, chain of from about 0 to 6 carbon equivalents in length, an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one of intermediate amide or disulfidemoieties, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. Group Z is preferably selected from an unbranched alkyl chain of general formula (CH 2 ) n , wherein n=1 to 6. 
     Group R 3  is selected from one of an H, an alkyl, and a methylene or ethylene moiety with an electronegative substitutent. An electronegative substituent is a substituent with a negative dipole moment, e.g. CN, COOH,etc. 
     Group R 3  is preferably selected from one of H, CH 3 , CH 2  CN, CH 2  COOH, CH 2  CONH 2 , CH 2  CH 2  OH and CH 2  OCH 3 . When group R 3  is H, group R 1  is preferably selected from one of acrylamide, amino, dithiopyridyl, hydrazide, imidate ester, maleimide, and thiol moieties. 
     The reagent of General Formula III is condensed with the bioactive species to yield a conjugate of General Formula IV: ##STR7##wherein groups Z and BAS are as defined above, and R 3  is selected fromone of an H, an alkyl, and a methylene or ethylenemoiety with an electronegative substitutent. 
     The conjugate of General Formula IV is then complexed with a phenylboronic acid conjugate having the general formula of General Formula V: ##STR8##wherein the symbol labeled BAS* represents a second biologically active species, that may include a linker portion and differ from the biologically active species labeled BAS of the complexing reagent. The BAS* may also include a portion of a reactive moiety used to attach the bioactive species to the phenylboronic acid reagent. The complexation yields the stereoisomeric complex (tetrahedral boron) of General Formula VI: ##STR9## 
     An alternative three-step process which utilizes reagents of General Formula I for the preparation of bioconjugates is also summarized in FIG. 1. Initially, a reagent of General Formula I is selected that is comprisedof an appropriate reactive electrophilic or nucleophic group R 1  suitable for reaction with the desired biologically active species. ##STR10##Group R 1  is a reactive electrophilic or nucleophilic moiety suitable for reaction of the putative phenylboronic acid complexing reagent with a bioactive species. Group R 1  is preferably selected from, but not limited to, acrylamide, bromo, dithiopyridyl, bromoacetamide, hydrazide, N-hydroxysuccinimidyl ester, N-hydroxysulfosuccinimidyl ester, imidate ester, imadazolide, iodo, iodoacetamide, maleimide, amino and thiol moieties. 
     Group Z is a spacer selected from a saturated or unsaturated, preferably unbranched, chain of from about 0 to 6 carbon equivalents in length, an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one of intermediate amide or disulfidemoieties, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. Group Z is preferably selected from an unbranched alkyl chain of general formula (CH 2 ) n , wherein n=1 to 6. 
     Group R 2  is selected from an alkyl (e.g., methyl, ethyl, etc.) and a methylene bearing an electronegative substituent. An electronegative substitutent is a substituent with a negative dipole moment, e.g., CN, COOH, etc. Group R 2  is preferably selected from one of CH 3 , CH 2  CH 3 , CH 2  CN, CH 2  COOH, CH 2  CONH and CH 2  OCH 3 . 
     The next step in a three-step process in the preparation of bioconjugates is to condense the appropriate reagent with the bioactive species to yielda conjugate of General Formula II: ##STR11##In General Formula II, Z and R 2  are as defined above, and BAS represents a biologically active species which may or may not contain a portion of a reactive moiety (and any spacer) used to attach the biologically active species to the reagent. 
     Next, the conjugate is reacted with a hydroxylamine derivative of the general formula NH 2  OR 3 , wherein R 3  is selected from eitheran H, an alkyl (e.g., methyl, ethyl, etc.), or a methylene or ethylene withan electronegative moiety. Suitable hydroxylamine derivatives include, but not limited to, NH 2  OH, NH 2  OCH, NH 2  OCH 2  CN, NH 2 OCH 2  COOH, NH 2  OCH 2  CONH 2  and NH 2  OCH 2  CH 2  OH. The resulting conjugate has the general formula of General Formula IV: ##STR12##wherein groups Z and BAS are as defined above, and R 3  is selected fromone of an H, an alkyl, and a methylene moiety with an electronegative substitutent. 
     The conjugate of General Formula IV is then complexed with a phenylboronic acid having the general formula of General Formula V: ##STR13##wherein the symbol labeled BAS* represents a second biologically active species, that may include a linker portion and differ from the biologically active species labeled BAS of the complexing reagent. The BAS* may also include a portion of a reactive moiety used to attach the bioactive species to the phenylboronic acid reagent. The complexation yields the stereoisomeric complex (about tetrahedral boron) of General Formula VI: ##STR14## 
     SYNTHESIS OF PHENYLBORONIC ACID COMPLEXING REAGENTS OF GENERAL FORMULA III ##STR15## 
     Reagents of General Formula III are derived from either 4- or 5-methylsalicylic acid. In each instance, the reagent is ultimately prepared from a synthetic intermediate which is either an alkyl 4- or 5-aminomethylbenzoate. 
     FIG. 2 summarizes the preparation of alkyl 4- and 5-aminomethylbenzoates, synthetic intermediates leading to reagents of General Formula III, wherein R 2  is an alkyl group, e.g., methyl, ethyl, etc. FIG. 2 shows an example where R 2  is a methyl group. Initially, in step 1, either 4- or 5-methylsalicylic acid is esterified to afford the corresponding alkyl 4- or 5-methylsalicylate. In step 2, the ester is brominated with N-bromo-succinimide and benzoyl peroxide catalyst to afford the corresponding benzylbromide. In step 3, the benzyl bromide is alkylated with sodium azide to afford the corresponding benzyl azide. Finally, in step 4, the benzyl azide is subjected to paladium catalyzed hydrogenation in the presence of HCl to afford the corresponding benzyl amine hydrochloride. 
     FIG. 4 summarizes the preparation of 4- and 5-aminomethylsalicylhydroxamic acids, synthetic intermediates leading to reagents of General Formula III,wherein group R 3  is one of an alkyl or methylene or ethylene bearing an electronegative substituent. Initially, in step 1, an alkyl 4- or 5-aminomethylsalicylate, prepared as summarized in FIG. 2, is condensed with N-(benzyloxycarbonyl)oxy succinimide to afford the alkyl N-benzyloxycarbonyl protected 4- or 5-aminomethylsalicylate. In step 2, the phenolic hydroxyl moiety was condensed with benzyl bromide to afford the further protected benzyl ether intermediate. In step 3, the alkyl ester is selectively cleaved by reaction with LiOH to afford the corresponding carboxylic acid. In step 4, the carboxylic acid is activatedby reaction with isobutylchloroformate to form a mixed anhydride which is subsequently reacted with a hydroxylamine derivative preferably selected from, but not limited to, either NH 2  OH, NH 2  OCHO 3 , NH 2  OCH 2  CN, NH 2  OCH 2  COOH, NH 2  OCH 2  CONH 2 , NH 2  OCH 2  CH 2  OH and NH 2  OCH 2  OCH 3  to afford the corresponding protected hydroxamic acid. Finally, in step 5, both the amine and phenolic hydroxyl moieties are simultaneously deprotected by paladium catalyzed hydrogenation in the presence of HCl to afford the corresponding 4- or 5-aminomethylsalicylhydroxamic acid hydrochloride. 
     FIG. 5 summarizes the synthesis of reagents of General Formula III, whereingroup R 3  is preferably selected from one of H, CH 3 , and a methylene or ethylene moiety with an electronegative substitutent, and wherein group R 1  is selected from either imidazolide, hydrazide and N-hydroxysuccinimidyl ester (in this instance, R 3  must be other than H) moieties. These reagents are each prepared by a two-step process in which an aliphatic acid anhydride is utilized in the first step. Initially, a 4- or 5-aminomethylsalicylhydroxamic acid, prepared as summarized in FIG. 4, is condensed of an aliphatic acid anhydride preferably selected from, but not limited to, either succinic anhydride, glutaric anhydride, maleic anhydride and glycolic acid anhydride, in an aprotic organic solvent, which results in the introduction of a spacer (group Z) having a free terminal carboxylic acid moiety. In the case wherethe aliphatic acid anhydride is maleic anhydride in this condensation reaction, the resulting Z group is unsaturated as it contains an alkene group. Subsequently, the carboxylic acid moiety is further functionalized by reaction with either N,N-carbonyldiimidazole, isobutylchloroformate andtert-butyl carbazate, or N,N-dicyclohexylcarbodiimide and N-hydroxysuccinimide to afford the corresponding imidazolide, protected hydrazide and N-hydroxysuccinimidyl ester, respectively. In the instance of the protected hydrazide, the N-(tert-butoxycarbonyl) protecting group is removed by contacting the reagent with anhydrous hydrogen chloride. 
     FIG. 6 summarizes the synthesis of reagents of General Formula III, whereingroup R 3  is preferably selected from one of H, CH 3 , and a methylene or ethylene moiety with an electronegative substitutent, and wherein group R 1  is selected from either bromo, maleimide, dithiopyridyl and imidate ester moieties. Reagents of General Formula III,wherein group R 1  is selected from either bromo and chloro moieties, are prepared by condensing a 4- or 5-aminomethylsalicylhydroxamic acid, prepared as summarized in FIG. 4, with either bromoacetic acid anhydride or chloroacetic acid anhydride, respectively. The homologous iodo reagent is prepared by halogen exchange of the chloro reagent with sodium iodide. Reagents of General Formula III, wherein R1 is selected from either bromo,chloro, iodo, bromoacetamide, chloroacetamide and iodoacetamide moieties, may not be conveiniently prepared when R3 is H, due to the potential for intermolecular allylation of the unprotected hydroxamate. Reagents of General Formula III, wherein group R 1  is selected from either maleimide and dithiopyridyl moieties, are prepared by condensing a 4- or 5-aminomethylsalicylhydroxamic acid, prepared as summarized in FIG. 4, with an N-hydroxysuccinimidyl ester of an aliphatic carboxylic ester whichbears either a terminal maleimide or dithiopyridyl moiety. Reagents of General Formula III, wherein R 1  is an imidate ester moiety, are prepared by a two-step process in which a 4- or 5-aminomethyl-salicylhydroxamic acid, prepared as summarized in FIG. 4, isfirst condensed with an N-hydroxysuccinimidyl ester of an aliphatic carboxylic ester which bears a terminal nitrile moiety. Subsequently, the nitrile moiety is converted to the methyl imidate ester by reaction with anhydrous hydrogen chloride in methanol at 0° C. 
     Another reagent of the present invention, which is the acrylic acid amide of aminomethyl-salicylhydroxamic acid, can be prepared in a single step, using the end product of FIG. 4, by condensing acrylic acid anhydride or acryloyl chloride with aminomethyl-salicylhydroxamic acid, provided that R3 is not H. Reagents of General Formula III, wherein group R 1  is selected from either N-hydroxy-succinimidyl ester and dithiopyridyl moieties may be utilized as synthetic intermediates to prepare reagents ofGeneral Formula III, wherein group Z is an unbranched saturated or unsaturated chain with at least one of intermediate amide or disulfide moieties. 
     Reagents of General Formula III, wherein group R 1  is an N-hydroxysuccinimidyl ester moiety, may be condensed with compounds havingprimary aliphatic amine moieties of the general formula R 1  -Z 2  -NH 2 , wherein 4 is an unbranched saturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents in length, to afford reagents of General Formula III, wherein group Z is an unbranched saturated or unsaturated chain with at least one of intermediate amide moieties. 
     Alternatively, N-hydroxysuccinimidyl ester reagents of General Formula III,preferably derived from a dicarboxylic acid selected from either succinic acid, maleic acid, fumaric acid, acetylenedicarboxylic acid and glutaric acid, may be condensed with compounds having primary aliphatic amine moieties of the general formula HO2C-Z 2  -NH 2 , wherein Z 2  isan unbranched saturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents in length, preferably selected from, either glycine, β-alanine, amniniopropiolic acid, 4-amino-butyric acid and 6-aminocaproic acid, to afford compounds having free terminal carboxylic acid moieties which may be further functionalized in accordance with FIG. 5 to afford reagents of General Formula III, wherein Z is an unbranched saturated or unsaturated chain with at least one of intermediate amide moieties. This process is summarized for in FIG. 7 for the synthesis of a reagent of General Formula III, wherein R 1  is an N-hydroxysuccinimidyl ester, and wherein Z is an unbranched saturated or unsaturated chain with at least one of an intermediate amide moiety. 
     Reagents of General Formula III, wherein group R 1  is a dithiopyridyl moiety, may be condensed with compounds having terminal thiol moieties of the general formula R 1  -Z 2  -SH, wherein Z 2  is an unbranchedsaturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents in length, to afford reagents of General Formula III, wherein group Z is an unbranched saturated or unsaturated chain with at least one of intermediate disulfide moieties. 
     Alternatively, dithiopyridyl reagents of General Formula III, preferably derived from a mercaptocarboxylic acid selected from either mercaptoaceticacid, β-mercaptopropionic acid, mercaptopropiolic acid, 4-mercaptobutyric acid and 6-mercaptocaproic acid, may be condensed with compounds having a terminal thiol moiety of the general formula HO 2  C-Z 2  -SH, wherein Z 2  is an unbranched saturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents in length, preferably selected from either mercaptoacetic acid, β-mercaptopropionic acid, mercaptopropiolic acid, 4-mercaptobutyric acid and 6-mercaptocaproic acid, to afford compounds having free terminal carboxylic acid moieties which may be further functionalized in accordancewith FIG. 5 to afford reagents of General Formula III, wherein group Z is an unbranched saturated or unsaturated chain with at least one of intermediate disulfide moieties. This process is summarized in FIG. 7 for the synthesis of a reagent of General Formula III, wherein R 1  is an N-hydroxysuccinimidyl ester, and wherein Z is an unbranched saturated or unsaturated chain with at least one of a intermediate disulfide moiety. 
     Reagents of General Formula III, wherein group Z is a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length, are prepared by condensing an allyl 4- or 5-aminomethylsalicylate, prepared as summarized in FIGS. 2 and 3, with a polyethylene glycol reagent having both an N-hydroxysuccinimidyl ester moiety and either a reactive electrophilic or nucleophilic moiety (or a precursor thereof), many of which are commercially available, to afford reagents of General Formual III, whereingroup Z is a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. 
     SYNTHESIS OF PUTATIVE PHENYLBORONIC ACID COMPLEXING REAGENTS OF GENERAL FORMULA I ##STR16## 
     Reagents of General Formula I are also derived from either 4- or 5-methylsalicylic acid. Once again, the reagent is ultimately prepared from a synthetic intermediate which is either an alkyl 4- or 5-aminomethylsalicylate. 
     FIG. 2 summarizes the preparation of alkyl 4- and 5-aminomethylsalicylates,synthetic intermediates leading to reagents of General Formula I, wherein R 2  is an alkyl group, e.g., methyl, ethyl, etc. FIG. 2 shows an example where R 2  is a methyl group. Initially, in step 1, either 4- or 5-methylsalicylic acid is esterified to afford the corresponding alkyl 4- or 5-methylsalicylate. In step 2, the ester is bromonated with N-bromo-succinimide and benzoyl peroxide catalyst to afford the corresponding benzylbromide. In step 3, the benzyl bromide is alkylated with sodium azide to afford the corresponding benzyl azide. Finally, in step 4, the benzyl azide is subjected to palladium catalyzed hydrogenationin the presence of HCl to afford the corresponding benzyl amine hydrochloride. 
     FIG. 3 summarizes the preparation of synthetic intermediates leading to reagents of General Formula I, wherein group R 2  is a methylene bearing an electronegative moiety, for example, carboxymethyl, acetamidomethyl and cyanomethyl 4- or 5-aminomethylsalicylate. It is to beappreciated that other contemplated substituents for group R 2  may be prepared by a similar synthesis. Initially, in step 1 of FIG. 3, the alkyl4- or 5-aminomethylsalicylate, prepared as summarized in FIG. 2, is reactedwith di-tert-butyl dicarbonate and triethylamine in methanol to afford the corresponding protected methyl N-(tert-butoxycarbonyl)aminomethyl-benzoate. In step 2, the methyl ester is cleaved by reaction with potassium trimethylsilanolate and worked up inaqueous acid to afford the corresponding benzoic acid. In step 3, the benzoic acid is alkylated by reaction with either an α-haloacid, α-haloacetamide or α-haloacetonitrile and triethylamine to afford the corresponding carboxymethyl, acetamido-methyl or cyanomethyl ester, respectively. Finally, in step 4, the N-(tert-butoxycarbonyl) protecting group is removed by reaction with anhydrous HCl in tetrahydrofuran to afford the corresponding benzyl amine hydrochloride. 
     FIG. 5 summarizes the synthesis of reagents of General Formula I, wherein group R 2  is one of either an alkyl or a methylene bearing an electronegative moiety, and wherein group R 1  is selected from either imidazolide, hydrazide and N-hydroxysuccinimidyl ester moeties. These reagents are each prepared by a two-step process in which an aliphatic acid anhydride is utilized in the first step. Initially, an alkyl 4- or 5-aminomethylsalicylate, prepared as summarized in FIGS. 2 and 3, is condensed of an aliphatic acid anhydride preferably selected from, but notlimited to, either succinic anhydride, glutaric anhydride, and glycolic acid anhydride, in an aprotic organic solvent, which results in the introduction of a spacer (group Z) having a free terminal carboxylic acid moiety. Subsequently, the carboxylic acid moiety is further functionalizedby reaction with either N,N-carbonyldiimidazole, isobutylchloroformate and tert-butyl carbazate, or N,N-dicyclohexylcarbodiimide and N-hydroxysuccinimide to afford the corresponding imidazolide, protected hydrazide and N-hydroxysuccinimidyl ester, respectively. In the instance of the protected hydrazide, the N-(tert-butoxycarbonyl) protecting group is removed by contacting the reagent with anhydrous hydrochloric acid. 
     FIG. 6 summarizes the synthesis of reagents of General Formula I, wherein group R 2  is one of either an alkyl or a methylene bearing an electronegative moiety, and wherein group R 1  is selected from either bromo, chloro, maleimide, dithiopyridyl and imidate ester moieties. Reagents of General Formula I, wherein group R 1  is selected from either bromo and chloro moieties, are prepared by condensing an alkyl 4- or 5-aminomethylsalicylate, prepared as summarized in either FIG. 2 or FIG. 3, with either bromoacetic acid anhydride or chloroacetic acid anhydride, respectively. The homologous iodo reagent is prepared by halogen exchange of the chloro reagent with sodium iodide. Reagents of General Formula I, wherein R 1  is selected from either bromo, chloro, iodo, bromoacetamide, chloroacetamide and iodoacetamide moieties, may not be conveiniently prepared when R 3  is H, due to the potential for intermolecular alkylation of the unprotected hydroxamate. Reagents of General Formula I, wherein group R 1  is selected from either maleimideand dithiopyridyl moieties, are prepared by condensing an alkyl 4- or 5-aminomethyl-salicylate, prepared as summarized in FIGS. 2 and 3, with anN-hydroxysuccinimidyl ester of an aliphatic carboxylic ester which bears either a terminal maleimide or dithiopyridyl moiety. A reagent of General Formula I, wherein R 1  is an imidate ester moiety, is prepared by a two-step process in which an alkyl 4- or 5-aminomethylsalicylate, preparedas summarized in FIGS. 2 and 3, is first condensed with an N-hydroxysuccinimidyl ester of an aliphatic carboxylic ester which bears aterminal nitrile moiety. Subsequently, the nitrile moiety is converted to the methyl imidate ester by reaction with anhydrous hydrochloric acid in methanol at 0° C. 
     Reagents of General Formula I, wherein group R 1  is selected from either dithiopyridyl (FIG. 6), imidazolide (FIG. 5) and N-hydroxysuccinimidyl ester (FIG. 5) moieties may be utilized as syntheticintermediates to prepare reagents of General Formula I having extended spacers, wherein group Z is an unbranched saturated or unsaturated chain with at least one of intermediate amide or disulfide moieties. 
     Reagents of General Formula 1, wherein group R 1  is selected from either imidazolide and N-hydroxysuccinimidyl ester moieties, may be condensed with compounds having primary aliphatic amine moieties of the general formula R 1  -Z&#39;-NH 2 , wherein Z&#39; is an unbranched saturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents in length, to afford reagents of General Formula I, wherein group Z is an unbranched saturated or unsaturated chain with at least one of intermediate amide moieties. Note that Z&#39; is shown as Z 2  in FIG. 7. 
     Alternatively, reagents of General Formula I, wherein group R 1  is selected from either imidazolide and N-hydroxysuccinimidyl ester moieties,may be condensed with compounds having primary aliphatic amine moieties of the general formula H 2  N-Z&#39;-COOH, wherein Z&#39; is an unbranched saturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents in length, preferably selected from, but not limited to, either glycine, β-alanine, 4-aminobutyric acid and 6-aminocaproic acid, to afford compounds having a free terminal carboxylic acid moiety which may be further functionalized in accordance with FIG. 5 to afford reagents of General Formula I, wherein Z is an unbranched saturated or unsaturated chain with at least one of intermediate amide moieties. 
     Reagents of General Formula I, wherein group R 1  is a dithiopyridyl moiety, may be condensed with compounds having terminal thiol moieties of the general formula R 1  -Z&#39;-SH, wherein Z&#39; is an unbranched saturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents inlength, to afford reagents of General Formula I, wherein group Z is an unbranched saturated or unsaturated chain with at least one of intermediate disulfide moieties. 
     Alternatively, reagents of General Formula I, wherein group R 1  is a dithiopyridyl moiety, may be condensed with compounds having terminal thiol moieties of the general formula HS-Z&#39;-COOH, wherein Z&#39; is an unbranched saturated or unsaturated chain preferably of from about 1 to 5 carbon equivalents in length, to afford compounds having a free terminal carboxylic acid moiety which may be further functionalized in accordance with FIG. 5 to afford reagents of General Formula I, wherein group Z is anunbranched saturated or unsaturated chain with at least one of intermediatedisulfide moieties. 
     Reagents of General Formula I, wherein group Z is a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length, are prepared by condensing an alkyl 4- or 5-aminomethylsalicylate, prepared as summarized in FIGS. 2 and 3, with a polyethylene glycol reagent having both an N-hydroxysuccinimidyl ester moiety and either a reactive electrophilic or nucleophilic moiety (or a precursor thereof), many of which are commercially available, to afford reagents of General Formula I, wherein group Z is a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. 
     PREPARATION OF PHENYLBORONIC ACID COMPLEXING CONJUGATES OF GENERAL FORMULA IV 
     At this point, the phenylboronic acid complexing reagents of General Formula III may be reacted with a suitable biologically active species to yield the conjugate of the general formula of General Formula IV: ##STR17## 
     Alternatively, conjugates of General Formula IV may also be prepard by the route which is decribed in FIG. I which utilizes a reagent of General Formula I: ##STR18## 
     Putative phenylboronic acid complexing reagents of General Formula I may bereacted with a suitable biologically active species to yield the conjugate of the general formula of General Formula II: ##STR19##The conjugate of General Formula II is next condensed with a hydroxylamine derivative to yield the corresponding phenylboronic acid complexing conjugate of General Formula IV: ##STR20## 
     Suitable hydroxylamine derivatives are preferably selected from, but not limited to, NH 2  OH, NH 2  OCH 3 , NH 2  OCH 2  CN, NH 2  OCH 2  COOH, NH 2  OCH 2  CONH 2 , NH 2  OCH 2 CH 2  OH, NH 2  OCH 2  OCH 3 . When group R 2  in General Formula II is an alkyl group, NH 2  OH is preferably utilized to effectthe interconversion of General Formula II to General Formula IV. 
     PREPARATION OF BIOCONJUGATES OF GENERAL FORMULA VI 
     Bioconjugates of General Formula VI may be prepared in buffered aqueous solutions or organic solvents. Preferred buffers include acetate, citrate,phosphate, carbonate and diglycine. Borate buffers should be avoided due totheir ability to complex with the phenylboronic acid complexing moiety. Tris, β-hydroxyamine and β-hydroxyacid buffers should be avoideddue to their ability to complex with the phenylboronic acid. The bioconjugate is formed within a few minutes over a range of temperatures of from about 4° C. to 70° C. The stability of the bioconjugate in aqueous solution at a given pH and temperature is influenced, to some extent, by substituent group R 3 . Bioconjugates ofGeneral Formula VI are stable in aqueous solutions of approximate pH greater than 3.5 and less than 10.5. The bioconjugation reaction (phenylboronic acid complexation) is insensitive to significant variationsin ionic strength over the range 0.01 to 1M, the presence of organic solvents including acetonitrile, methanol, ethanol, isopropanol, butanol, N,N-dimethylformamide and dimethylsulfoxide, the presence of detergents, and the presence of chaotropic agents (protein denaturants) including urea, guanidine hydrochloride, guanidine thiocyanate and formamide, which are incompatible with prior art indirect labeling systems wherein the structure of a biological macromolecule must be maintained to preserve requisite binding properties. Once formed, the bioconjugates are stable upon removal of water, and can be lyophilized for storage. 
     The stability of the bioconjugate at a given pH is determined to some extent by substituent group R 3 . Phenylboronic acid complexes of General Formula VI, wherein group R 3  is H, are stable in buffered aqueous solutions over the approximate pH range 3.5 to 10.5. Phenylboronicacid complexes of General Formula VI, wherein group R 3  is CH 3 , are stable in buffered aqueous solutions over the approximate pH range 4.5to 10.5. Phenylboronic acid complexes of General Formula VI, wherein group R 3  includes an electronegative moiety, are stable in buffered aqueoussolutions over the approximate pH range less than 3.5 to 10.5. 
     The stability of the phenylboronic acid complex toward acid catalyzed hydrolysis is related to the pK a  of the hydroxamic acid participatingin the complex. The lower the pK a  of the hydroxamic acid moiety the more stable the complex. Consequently, phenylboronic acid complexes of General Formula VI wherein group R 3  includes an electronegative moiety exhibit greater stability toward acid catalyzed hydrolysis than do those in which R 3  is either H or CH 3 . 
     The following examples present a detailed description of the synthesis of reagents of General Formula I and General Formula III. 
     EXAMPLE I 
     Preparation of Ethyl 4-Aminomethylsylicylate Hydrochloride ##STR21## 
     Ethyl 4-Methylsalicylate 
     4-Methylsalicylic acid (20.0 g, 131 mmoles) was dissolved in ethanol (300 mL) and concentrated sulfuric acid (2.0 mL) was added. The mixture was refluxed for 40 hours. The volume of the reaction mixture was reduced to 100 mL, transferred to a separatory funnel, and diluted with chloroform (250 mL) and water (200 mL). Solid sodium bicarbonate was added in small portions until the pH of the aqueous layer was about 8 (pH test paper). The mixture in the funnel was shaken well and the layers separated. The organic layer was washed first with water (150 mL) and then saturated aqueous sodium chloride (150 mL). Finally, the organic solution was dried over anhydrous magnesium sulfate, filtered, and the solvent evaporated to afford 14.0 g (59% yield) of liquid ethyl 4-methylsalicylate. 
       1  H NMR (300 MHz, CHCl 3  -d) δ1.40 (triplet, J=7 Hz, 3H, CH 2  CH 3 ), 2.33 (singlet, 3H, ArCH 3 ), 4.38 (quartet, J=7 Hz,2H, CH 2  CH 3 ), 6.67 (doublet, J=8 Hz, 1H, ArH), 6.78 (singlet, 1H,ArH), 7.72 (doublet, J=8 Hz, 1H, ArH), 10.81 (singlet, 1H, OH).  13  C NMR (75 MHz, CHCl 3  -d) δ14.0, 21.7, 61.1, 110.1, 117.7, 120.4, 129.7, 146.9, 161.7, 170.3. 
     Ethyl 4-Bromomethylsalicylic Acid 
     Ethyl 4-methylsalicylate (13.1 g, 72.5 mmoles) was dissolved in carbon tetrachloride (150 mL) and N-bromosuccinimide (13.1 g, 73.2 mmoles) and benzoyl peroxide (0.2 g, 0.8 mmoles) were added. The mixture was refluxed for 3.5 hours and then allowed to cool to room temperature. The solids were removed by filtration and the filtrate was evaporated to dryness. Thecrude solid product was crystallized from hexane (100 mL) to afford 5.0 g (27% yield) of ethyl 4-bromomethylsalicylic acid (m.p. 64°-66° C.). 
       1  H NMR (300 MHz, CHCl 3  -d) δ1.41 (triplet, J=7 Hz, 3H, CH 2  CH 3 ), 4.40 (quartet, J=7 Hz, 2H, CH 2  CH 3 ), 4.40 (singlet, 2H, CH 2  Br), 6.90 (doublet, J =8 Hz, 1H, ArH), 6.99 (singlet, 1H, ArH), 7.82 (doublet, J=8 Hz, 1H, ArH), 10.88 (singlet, 1H, OH).  13  C NMR (75 MHz, CHCl 3  -d) δ14.0, 31.9, 61.5, 112.4,117.9, 119.8, 130.5, 145.5, 161.8, 169.9. 
     Ethyl 4-Aminomethylsalicylate Hydrochloride 
     Ethyl 4-bromomethylsalicylate (4.8 g 18.6 mmoles) was dissolved in dry N,N-di-methylformamide (50 mL) and sodium azide (1.2 g 18.9 mmoles) was added. The suspension was stirred at room temperature for 2 hours. The reaction mixture was then diluted with dichloromethane (150 mL) and extracted with 1N aqueous hydrochloric acid (100 mL), water (100 mL), and saturated aqueous sodium chloride (50mL). Finally, the solution was then dried over anhydrous magnesium sulfate, filtered, and evaporated to dryness to give ethyl 4-azidomethylsalicyalte as an oil. 
     Palladium on carbon (0.5 g 10%  w/w!) was added to a 1L hydrogenation flaskunder a nitrogen atmosphere. The crude ethyl 4-azidomethylsalicylate was dissolved in ethanol (200 mL) and transferred to the hydrogenation flask. Concentrated aqueous hydrochloric acid (2 mL) was then added, and the flask was affixed to the Parr hydrogenator. The reaction mixture was shaken under 35 psi of hydrogen for 4 hours at room temperature. The mixture was then filtered through Celite to remove the catalyst, and the filtrate was evaporated to dryness to afford an off-white solid. Finally, this solid was crystallized from EtOH to afford 3.1 g (71% yield) of ethyl4-aminomethylsalicylate hydrochloride (m.p. 240°-241° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.30 (triplet, J=7 Hz, 3H, CH 2  CH 3 ), 3.99 (singlet, 2H, CH 2  NH 3 ), 4.33 (quartet,J=7 Hz, 2H, CH 2  CH 3 ), 7.06 (doublet, J=8 Hz, 1H, ArH), 7.15 (singlet, 1H, ArH), 7.77 (doublet, J=8 Hz, 1H, ArH), 8.71 (broad singlet, 3H, NH 3 ), 10.62 (broad singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ14.0, 38.7, 41.6, 61.5, 112.9, 117.7, 119.8, 130.3, 142.2, 160.2, 168.9. 
     EXAMPLE II 
     Preparation of a Reagent of General Formula I Ethyl N-Iodoacetyl-4-aminomethylsalicylate ##STR22## 
     Ethyl N-Chloroacetyl-4-aminomethylsalicylate 
     Ethyl 4-aminomethylsalicylate hydrochloride (0.50 g. 2.17 mmoles) was suspended in dry N,N-dimethylformamide (25 mL) and N,N-diisopropylethylamine (0.38 mL, 2.18 mmoles) was added. Once the aminesalt dissolved, chloroacetic anhydride (0.39 g, 2.25 mmoles) was added and the reaction mixture was stirred at room temperature for 4.5 hours. The reaction mixture was then diluted with ethyl acetate (100 mL), and this solution was extracted with 1N aqueous hydrochloric acid (100 mL), water (50 mL), and saturated aqueous sodium chloride (50 mL). The ethyl acetate solution was dried over anhydrous magnesium sulfate, filtered and evaporated to dryness to afford a white solid. Finally, this solid was crystallized from ethyl acetate:hexanes (8:2, 10 mL) to affored 0.13 g (22% yield) of ethyl N-chloroacetyl-4-aminomethylsalicylate (m.p. 120°-121° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.31, (triplet, J=7 Hz, 3H, CH 2  CH 3 ), 4.14 (singlet, 2H, ClCH 2 ), 4.29 (doublet, J =6 Hz, 2H, NHCH 2 ), 4.34 (quartet, J =7 Hz, 2H, CH 2  CH 3 ), 6.82 (doublet, J=8 Hz, 1H, ArH), 6.84 (singlet, 1H, ArH), 7.73 (doublet, J=8 Hz, 1H, ArH), 8.78 (triplet,J=6 Hz, 1H, NH), 10.58 (singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ13.9, 42.1, 42.6, 61.3, 111.6,115.6, 118.4, 130.1, 147.6, 160.6, 166.5, 169.1. 
     Ethyl N-Iodoacetyl-4-aminomethylsalicylate 
     Ethyl N-chloroacetyl-4-aminomethylsalicylate (0.11 g, 0.40 mmoles) was dissolved in acetone (5 mL) and sodium iodide (0.06 g, 0.40 mmoles) was added. The solution was refluxed for 2.5 hours, then cooled to room temperature and filtered. The filtrate was evaporated to dryness to afforda white solid of ethyl N-iodoacetyl-4-aminomethylsalicylate (0.19 g, 100% yield) (m.p. 105°-108° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.31, (triplet, J=7 Hz, 3H, CH 2  CH 3 ), 3.68 (singlet, 2H, ICH 2 ), 4.26 (doublet, J=6 Hz, 2H, NHCH 2 ), 4.33 (quartet, J=7 Hz, 2H, CH 2  CH 3 ), 6.82 (doublet, J=8 Hz, 1H, ArH), 6.84 (singlet, 1H, ArH), 7.72 (doublet, J=8 Hz, 1H, ArH), 8.79 (triplet, J=6 Hz, 1H, NH), 10.58 (singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ0.41, 13.9, 42.0, 61.3, 111.4,115.5, 118.3, 130.1, 147.9, 160.6, 168.3, 169.1. 
     EXAMPLE III 
     Preparation of a Reagent of General Formula I Ethyl (6-Aminohexanoyl)aminomethylsalicylate Trifluoroacetate ##STR23## 
     Ethyl (N-tert-Butoxycarbonyl-6-aminohexanoyl)aminomethylsalicylate 
     Ethyl 4-aminomethylsalicylate hydrochloride (0.52 g 1.28 mmoles) was suspended in anhydrous N,N-dimethylformamide (25 mL), and N,N-diisopropylethylamine (0.79 mL, 4.53 mmoles) was added, followed by N-tert-butoxycarbonyl-6-aminohexanoic acid succinimidyl ester (0.74 g 2.26mmoles). The mixture was stirred under dry nitrogen for 18 hours, during which time all solids dissolved. The reaction mixture was diluted with ethyl acetate (100 mL) and extracted with 1N aqueous hydrochloric acid (100 mL). The layers were separated, and the ethyl acetate solution was washed with water (100 mL) and saturated aqueous sodium chloride (500 mL).The ethyl acetate solution was dried over anhydrous magnesium sulfate, filtered, and evaporated to afford an amorphous off-white solid. Finally, the solid was crystallized from ethyl acetate, filtered, and dried in vacuo to afford 0.67 g (73% yield) of ethyl (N-tert-butoxycarbonyl-6-aminohexanoyl)aminomethylsalicylate (m.p. 120°-121° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.19 (multiplet, 2H, NHCH 2 CH 2  CH 2  CH 2  CH 2  CO), 1.34 (multiplet, 5H, CH 2  CH 2  CO and CH 2  CH 3 ), 1.34 (singlet, 9 H, C(CH 3 ) 3 ), 1.49 (multiplet, 2 H, NHCH 2  CH 2 ), 2.12 (triplet, J 7 Hz, 2 H, CH 2  CH 2  CO), 2.87 (quartet, J =6 Hz, 2 H,NHCH 2  CH 2 ), 4.23 (doublet, J=6 Hz, 2 H, CH 2  Ar), 4.32 (quartet, J=7 Hz, CH 2  CH 3 ), 6.74 (triplet, J=6 Hz, 1 H, CONHCH 2  CH 2 ), 6.80 (doublet, J=8 Hz, 1 H, ArH), 6.81 singlet, 1 H, ArH), 7.71 (doublet,J=8 Hz, 1 H, ArH), 8.34 (triplet, J=6 Hz, 1 H, CONHCH 2  Ar), 10.58 (singlet, 1 H, OH). 13C NMR (75 MHz, DMSO-d 6 ) δ14.0, 25.1, 26.3, 28.3, 29.7, 36.3, 40.2, 42.9, 61.4, 79.0, 111.6, 116.0, 118.3, 130.4, 146.9, 156.2, 161.9, 170.1, 173.0. 
     Ethyl (6-Aminohexanoyl)aminomethylsalicylate Trifluoroacetate 
     Ethyl (N-tert-butoxycarbonyl-6-aminohexanoyl)aminomethylsalicylate (0.58 g,1.41 mmoles) was dissolved in dichloromethane (5 mL) and the solution was cooled in an ice/water bath. Trifluoroacetic acid (5 mL) was added, and the reaction was allowed to warm to room temperature. After 2 hours, the reaction mixture was evaporated to dryness to give the product as an oil, which was dried in vacuo over potassium hydroxide pellets to afford 0.59 g(99% yield) of ethyl (6-aminohexanoyl)aminomethylsalicylate trifluoroacetate. 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.28 (multiplet, 5H, H 3  CH 2  CH 2  CH 2  CH 2  CH 2  CO and CH 2  CH 3 ), 1.53 (multiplet, 4H, NH 3  CH 2  CH 2  CH 2  CH 2  CH 2 CO), 2.15 (triplet, J=8 Hz, 2 H, CH 2  CH 2  CO), 2.71 (multiplet, 2 H, NH 3  CH 2  CH 2 ), 4.21 (doublet, J=6 Hz, 2 H, CH 2  Ar), 4.30 (quartet, J=8 Hz, 2H, CH 2  CH 3 ), 6.79 (doublet,J=8 Hz, 1 H, ArH), 6.81 (singlet, 1 H, ArH), 7.68 (doublet, J=8 Hz, 1 H, ArH), 8.18 (broad singlet, 3 H, NH 3 ), 8.60 (triplet, J=6 Hz, 1 H, CONHCH 2  Ar), 10.58 (broad singlet, 1 H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ14.0, 24.8, 25.6, 26.7, 35.1, 38.6, 41.7, 61.3, 111.5, 115.5, 118.3, 130.1, 148.6, 160.6, 169.2, 172.6. 
     EXAMPLE IV 
     Preparation of a Reagent of General Formula I Methyl 4-Succinylaminomethylsalicylate Succinimidyl Ester ##STR24## 
     Methyl 4-Methylsalicylate 
     4-Methylsalicylic acid (100 g, 658 mmoles) was dissolved in anhydrous methanol (500 mL) and concentrated sulfuric acid (25 mL) was added carefully. The solution was refluxed for 18 hours, then cooled to room temperature. The reaction mixture was concentrated to about 150 mL, and ethyl acetate (250 mL) was added. The ethyl acetate solution was washed twice with saturated aqueous sodium bicarbonate (250 mL portions) and thenwith saturated aqueous sodium chloride (100 mL). The ethyl acetate solutionwas dried over anhydrous sodium sulfate, filtered, and evaporated to a clear, reddish-brown liquid. This crude product was vacuum distilled (oil pump) to afford a clear, viscous liquid that solidified on standing to afford 98.1 g (90% yield) of methyl 4-methylsalicylate. 
       1  H NMR (300 MHz, CHCl 3  -d) δ2.32 (singlet, 3H, ArCH 3 ), 3.91 (singlet, 3H, OCH 3 ), 6.67 (doublet,J=8 Hz, 1H, ArH), 6.78 (singlet, 1H, ArH), 7.69 (doublet, J=8 Hz, 1H, ArH), 10.71 (singlet, 1H, OH).  13  C NMR (75 MHz, CHCl 3  d) δ21.8, 52.1,110.0, 117.9, 120.6, 129.9, 147.3, 161.9, 170.9. 
     Methyl 4-Bromomethylsalicylate 
     Methyl 4-methylsalicylate (98.1 g 590 mmoles) was dissolved in carbon tetrachloride (600 ml), and N-bromosuccinimide (105.0 g, 590 mmoles) and benzoyl peroxide (0.7 g, 3 mmoles) were added. The mixture was refluxed under nitrogen. After 2 hours, an additional portion (0.7 g) of N-bromosuccinimide was added. Reflux was continued for 16 hours. The reaction mixture was cooled to room temperature and the solid removed by filtration. The yellow filtrate was evaporated to dryness to afford a thick yellow syrup that solidified on standing. Hexanes (500 mL) was addedto the solid, and the mixture was boiled until almost all solids dissolved.The hot hexanes solution was filtered and concentrated until a solid just began to precipitate. The mixture was heated to dissolve the solid, and the solution was allowed to cool slowly to room temperature. Pale yellow crystals formed slowly. The mixture was then chilled in ice for 2 hours tocomplete crystallization. Finally, the solid was filtered, washed with coldhexanes (100 mL), and dried in vacuo to afford 83.5 g (58% yield) of methyl4-bromomethyl-salicylate (m.p. 73°-75° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, CHCl 3  d) δ3.95 (singlet, 3H, OCH 3 ), 4.40 (singlet, 2H, CH 2 ), 6.90 (doublet, J=8 Hz, 1H, ArH), 7.00 (singlet, 1H, ArH), 7.80 (doublet, J=8 Hz, 1H, ArH), 10.78 (singlet, 1H, OH).  13  C NMR (75 MHz, CHCl 3  d) δ32.1, 52.5, 112.4, 118.2,120.1, 130.7, 145.8, 162.0, 170.5. 
     Methyl 4-Azidomethylsalicylate 
     Methyl 4-bromomethyl salicylate (83.5 g, 341 mmoles) was dissolved in dry N,N-dimethylformamide (150 mL) and sodium azide 25.0 g, 380 mmoles) was added. The yellow suspension was stirred at room temperature, and the solids rapidly dissolved. The solution turned brown, and a precipitate of sodium bromide formed. The reaction mixture was stirred 16 hours, then filtered. The filtrate was evaporated to a brown oil, which was dissolved in a mixture of hexanes and ethyl acetate (1:1  v/v!. 100 mL). Silica gel (25 g, flash chromatography grade) was added to the brown solution, and the mixture was swirled well. The silica was removed by filtration on a glass frit, and washed three times with hexanes:ethyl acetate (1:1  v/v!, 50 mL portions). The silica gel was dried on the frit, and the combined filtrates were evaporated to dryness to afford a dark yellow liquid. The crude product (which was utilized for the following reaction) was found tocontain some residual N,N-dimethylformamide. 
       1  H NMR (300 MHz, CHCl 3  d) δ3.94 (singlet, 3H, OCH 3 ), 4.32 (singlet, 2H, CH 2 ), 6.82 (doublet,J=8 Hz, 1H, ArH), 6.93 (singlet, 1H, ArH), 7.83 (doublet, J=8 Hz, 1H, ArH), 10.80 (singlet, 1H, OH).  13  C NMR (75 MHz, CHCl3d) δ52.5, 54.3, 112.3, 117.0, 118.7, 130.8, 143.9, 162.1, 170.5. 
     Methyl 4-Aminomethylsalicylate Hydrochloride 
     Crude methyl 4-azidomethylsalicylate was dissolved in methanol (750 mL) in a 2L Parr hydrogenation flask. Palladium on carbon catalyst (10%  w/w!, 3.8 g) in water (25 mL) was added, followed by concentrated hydrochloric acid (35 mL). The flask was affixed to a Parr hydrogenator, and the mixture was shaken at room temperature under 40 psi of hydrogen for 16 hours. The reaction mixture was then filtered through a 0.45 mm nylon filter. The retained solid was then washed with methanol (150 mL), water (100 mL), and methanol again (150 mL). The combined filtrates were evaporated to dryness to afford a tan solid. This solid was dissolved in hot denatured ethanol (150 mL) and the solution was allowed to cool to room temperature. White crystals formed quickly. Finally, the mixture was then chilled for 16-18 hours at 4° C. to complete crystallization. The solid was filtered, washed with a little cold ethanol (50 mL) and thendiethyl ether (150 mL), and dried in vacuo over potassium hydroxide pelletsto afford 51.5 g (65% yield based on methyl 4-bromomethylsalicylate) of methyl 4-aminomethylsalicylate hydrochloride (m.p. 225°-227°C., open capillary, uncorrected). 
       1  H (300 MHz, DMSO-d 6 ) δ3.87 (singlet, 3H, OCH 3 ), 4.00(singlet, 2H, CH 2 ), 7.06 (doublet, J=8 Hz, 1H, ArH), 7.13 (singlet, 1H, ArH), 7.77 (doublet, J=8 Hz, 1H, ArH), 8.59 (broad singlet, 3H, NH 3  Cl), 10.55 (singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ41.6, 52.6, 113.0, 117.7, 119.8, 130.4, 142.1, 160.0,169.1 
     Methyl 4-Succinylaminomethylsalicylate 
     Methyl 4-aminomethylsalicylate hydrochloride (3.00 g, 13.8 mmoles) was suspended in dry pyridine (25 mL), and N,N-diisopropylethylamine (2.7 mL, 15.5 mmoles) was added. The suspension was stirred in an ice/water bath for 15 minutes, and then succinic anhydride (1.36 g, 13.6 mmoles) was added. The mixture was allowed to warm to room temperature, during which time the starting solids dissolved. After stirring for 2 hours, the mixture was evaporated to dryness, and the resulting solid was partitionedbetween ethyl acetate (300 mL) and 1M aqueous hydrochloric acid (100 mL). The layers were separated, and the ethyl acetate solution was washed with 1M hydrochloric acid (100 mL) and saturated aqueous sodium chloride (100 mL). The solution was dried over anhydrous sodium sulfate, filtered, and evaporated to dryness to yield a white solid. Finally, the solid was crystallized from ethyl acetate/hexanes, filtered, and dried in vacuo to afford 3.02 g (87% yield) of methyl 4-succinylaminomethylsalicylate (m.p. 161°-163° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ2.43 (triplet, J=6 Hz, 2H, CH 2  CONH), 2.49 (triplet, J=6 Hz, 2H, HO 2  CCH 2 ), 3.87 (singlet, 3H, OCH 3 ), 4.27 (doublet, J 6 Hz, 2H, ArCH 2  NH), 6.83 (doublet,J=8 Hz, 1H, ArH), 6.86 (singlet, 1H, ArH), 7.71 (doublet, J=8 Hz,1H, ArH), 8.43 (triplet,J=6 Hz, 1H, NH), 10.54 (singlet, 1H, OH), 12.12 (singlet, 1H, CO 2  H).  13  C NMR (75 MHz, DMSO-d 6 ) δ29.1, 30.1, 41.9, 52.5, 111.3, 115.6, 118.4, 130.1, 148.7, 160.7, 169.7, 171.7, 174.2. 
     Methyl 4-Succinylaminomethylsalicylate Succinimidyl Ester 
     Methyl 4-succinylaminomethylsalicylate (2.60 g, 10.2 mmoles) was dissolved in dry tetrahydrofuran (100 mL), and N-hydroxysuccinimide (1.29 g 11.2 mmoles) and 1,3-di-cyclohexylcarbodiimide (2.10 g, 10.2 mmoles) were added. The mixture was stirred at room temperature under dry nitrogen, andthe solids rapidly dissolved. After about 20 minutes, a white precipitate formed. The reaction mixture was stirred 16-18 hours, then chilled severalhours at -20° C. The mixture was filtered cold, and the solid washedwith a little tetrahydrofuran (25 mL). The combined filtrates were evaporated to dryness, and the residue was crystallized from ethyl acetate/hexanes, filtered, and dried in vacuo to afford 2.39 g (62% yield)of methyl 4-succinyl-aminomethylsalicylate succinimidyl ester (m.p. 133°-135° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ2.56 (triplet, J=7 Hz, 2H, CH 2  CONH), 2.80 (singlet, 4H, COCH 2  CH 2  CO), 2.91 (triplet,J=7 Hz, 2H, NO 2  CCH 2 ), 3.87 (singlet, 3H, OCH 3 ), 4.27 (doublet, J=6 Hz, 2H, ArCH 2  NH), 6.83 (doublet,J=9 Hz, 1H, ArH), 6.84(singlet, 1H, ArH), 7.71 (doublet, J=9 Hz, 1H, ArH), 8.51 (triplet, J=6 Hz,1H, NH), 10.50 (singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ25.4, 25.9, 29.2, 41.8, 52.4, 111.4, 115.6, 118.4, 130.2, 148.2, 160.4, 168.9, 169.4, 170.2, 170.4. 
     EXAMPLE V 
     Preparation of a Reagent of General Formula I 
     Methyl (6-Aminohexanoyl)aminomethylsalicylate Hydrochloride ##STR25## 
     Methyl (N-tert-Butoxycarbonyl-6-aminohexanoyl)aminomethylsalicylate 
     Methyl 4-aminomethylsalicylate hydrochloride (2.25 g, 10.3 mmoles) was suspended in anhydrous N,N-dimethylformamide (30 mL), and N,N-diisopropylethylamine (3.6 mL, 20.7 mmoles) was added, followed by N-tert-butoxycarbonyl-6-aminohexanoic acid succinimidyl ester (3.38 g, 10.3 mmoles). The mixture was stirred under dry nitrogen for 18 hours, during which time all solids dissolved. The solvent was then evaporated toleave a light brown syrup, which was partitioned between ethyl acetate (100mL) and 1M aqueous hydrochloric acid (100 mL). The layers were separated, and the ethyl acetate solution was washed with saturated aqueous sodium bicarbonate (100 mL) and saturated aqueous sodium chloride (100 mL). The ethyl acetate solution was dried over anhydrous sodium sulfate, filtered, and evaporated to an amorphous off-white solid. The solid was crystallizedform ethyl acetate/hexanes, filtered, and dried in vacuo to afford 3.25 g (80% yield) of methyl (N-tert-butoxycarbonyl-6-aminohexanoyl)-aminomethylsalicylate (m.p. 120°-121° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.22 (multiplet, 2H, NHCH 2 CH 2  CH 2  CH 2  CH 2  CO), 1.36 (multiplet, 2H, CH 2  CH 2  CO), 1.36 (singlet, 9 H, C(CH 3 ) 3 ), 1.51 (multiplet, 2 H, NHCH 2  CH 2 ), 2.13 (triplet, J=7 Hz, 2 H, CH 2  CH 2  CO), 2.87 (quartet, J=6 Hz, 2 H, NHCH 2  CH 2 ), 3.87 (singlet, 3 H,OCH 3 ), 4.24 (doublet, J=6 Hz, 2 H, CH 2  Ar), 6.75 (triplet, J=6 Hz, 1 H, CONHCH 2  CH 2 ), 6.80 (doublet, J=8 Hz, 1 H, ArH), 6.82 (singlet, 1 H, ArH), 7.72 (doublet, J=8 Hz, 1 H, ArH), 8.35 (triplet, J=6 Hz, 1 H, CONHCH 2  Ar), 10.53 (singlet, 1 H, OH).  13  C NMR (75 MHz, CHCl 3  -d) δ25.0, 26.0, 28.2, 29.3, 35.3, 41.7, 52.4, 77.4,111.2, 115.5, 118.3, 130.1, 148.7, 155.8, 160.5, 169.5, 172.6. 
     Methyl (6-Aminohexanoyl)aminomethylsalicylate Hydrochloride 
     Methyl (N-tert-butoxycarbonyl-6-aminohexanoyl)aminomethylsalicylate (3.00 g, 7.60 mmoles) was dissolved in ethyl acetate (100 mL), and dry hydrogen chloride was bubbled slowly through the solution. The reaction mixture warmed as the gas dissolved. After 5 minutes, the gas was shut off, and the solution was stirred at room temperature. A white precipitate formed in the solution. After 30 minutes, the reaction mixture was chilled in icefor 2 hours, then the solid was filtered, washed with diethyl ether (50 mL)and dried in vacuo over potassium hydroxide pellets to afford 2.50 g (99% yield) of methyl (6-aminohexanoyl)aminomethylsalicylate hydrochloride (decomposes with effervescence at 158°-160° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.28 (multiplet, 2H, H 3  CH 2  CH 2  CH 2  CH 2  CH 2  CO), 1.54 (multiplet, 4H, NH 3  CH 2  CH 2  CH 2  CH 2  CH 2  CO), 2.16 (triplet, J=8 Hz, 2 H, CH 2  CH 2  CO), 2.71 (multiplet, 2 H, NH 3  CH 2  CH 2 ), 3.85 (singlet, 3 H, OCH 3 ), 4.22 (doublet, J=6 Hz,2 H, CH 2  Ar), 6.80 (doublet, J=8 Hz, 1 H, ArH), 6.83 (singlet, 1 H, ArH), 7.70 (doublet, J=8 Hz, 1 H, ArH), 8.10 (broad singlet, 3 H, NH3), 8.54 (triplet, J=6 Hz, 1 H, CONHCH 2  Ar), 10.35 (broad singlet, 1 H, OH).  13  C NMR (75 MHz, CHCl 3  -d) δ24.8, 25.6, 26.7, 35.1, 41.7, 111.5, 115.6, 118.4, 130.2, 148.6, 160.4, 169.4. 
     EXAMPLE VI 
     Preparation of a Reagent of General Formula I Cyanomethyl 4-Glutarylaminomethylsalicylate Succinimidyl Ester ##STR26## 
     Methyl N-tert-Butoxycarbonylaminomethylsalicylate 
     Methyl 4-aminomethylsalicylate hydrochloride (10.9 g, 50 mmoles) was suspended in anhydrous methanol (200 mL) and di-tert-butyldicarbonate (10.9 g, 50 mmoles) and triethylamine (7.0 mL, 50 mmoles) were added. The solid rapidly dissolved with the slow evolution of gas. The reaction mixture was stirred at room temperature for 18 hours under dry nitrogen, then evaporated to dryness to afford a white amorphous mass. This mass waspartitioned between ethyl acetate (200 mL) and water (100 mL). The layers were separated, and the ethyl acetate solution was dried over anhydrous sodium sulfate. The solution was filtered and evaporated to a white solid.This solid was crystallized from ethyl acetate/hexanes, filtered, and driedin vacuo to afford 13.7 g (97% yield) of methyl N-tert-butoxycarbonylaminomethyl-salicylate (m.p. 95°-96° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, CHCl 3  -d) δ1.42 (singlet, 9H, C(CH 3 ) 3 ), 3.90 (singlet, 3H, OCH 3 ), 4.26 (doublet, J=6 Hz, 2 H, CH 2  Ar), 4.99 (triplet, J=6 H, 1H, NH), 6.75 (doublet, J=8 Hz, IH, ArH), 6.84 (singlet, 1 H, ArH), 7.73 (doublet, J=8 Hz, 1 H, ArH), 10.72 (singlet, 1 H, OH).  13  C NMR (75 MHz, CHCl 3  -d) δ28.5, 44.4, 52.4, 80.0, 111.5, 115.9, 118.2, 130.5, 150.0, 156.3, 162.1, 170.8. 
     N-tert-Butoxycarbonylaminomethylsalicylic Acid 
     Methyl N-tert-butoxycarbonylaminomethylsalicylate (8.7 g, 30.9 mmoles) was dissolved in dry tetrahydrofuran (100 mL), and potassium trimethylsilanolate (4.4 g, 30.9 mmoles, 90% pure) was added. The yellow solution was refluxed for 24 hours, during which time a tan precipitate formed and the solvent turned light brown. The mixture was evaporated to dryness, and the solid was dissolved in cold water (100 mL). The brown solution was chilled in an ice bath, and saturated aqueous potassium hydrogen sulfate solution was used to titrate the stirred solution to pH 2-3. An off-white solid precipitated during the titration. The solid was filtered, washed with cold water, and dried in vacuo over potassium hydroxide to afford 6.7 g. (81% yield) of crude N-tert-butoxycarbonylaminomethylsalicylic acid (m.p. 141°-144° C., decomposes with effervescence on melting, opencapillary, uncorrected). 
       1  H NMR (300 MHz, CHCl 3  -d) δ1.47 (singlet, 9H, C(CH 3 ) 3 ), 4.33 (doublet, J=6 Hz, 2 H, CH 2  Ar), 5.07 (triplet, J=6 H, 1H, NH), 6.80 (doublet, J=8 Hz, 1 H, ArH), 6.88 (singlet,1 H, ArH), 7.81 (doublet,J=8 Hz, 1 H, ArH), 10.72 (broad singlet, 2 H, OH and CO 2  H).  13  C NMR (75 MHz, CHCl 3  -d) δ28.5, 44.4, 80.5, 111.1, 115.9, 118.3, 131.5, 148.5, 156.6, 162.6, 174.1. 
     Cyanomethyl N-tert-Butoxycarbonylaminomethylsalicylate 
     N-tert-Butoxycarbonylamino-methylsalicylic acid (8.2 g, 30.6 mmoles) was suspended in chloroacetonitrile (25 mL), and triethylamine (4.3 mL, 30.6 mmoles) was added. The mixture was stirred under dry nitrogen at 50° C., and the solids dissolved. The solution was stirred 18 hours, and then cooled to room temperature. The solvent was evaporated, and the residue was partitioned between ethyl acetate (250 mL) and water (250 mL). The layers were separated, and the ethyl acetate layer was washed with saturated aqueous sodium bicarbonate (100 mL) and saturated aqueous sodium chloride (100 mL). The solution was dried over anhydrous sodium sulfate, filtered, and evaporated to dryness. The residual pale tansolid was dissolved in ethyl acetate (100 mL), and silica gel (10 g, flash chromatography grade) was added. The mixture was swirled well and allowed to sit for five minutes at room temperature. The silica was removed by filtration on a grlass frit, and washed with ethyl acetate (100 mL). The filtrate was evaporated to dryness. The solid residue was crystallized from ethyl acetate/hexanes to afford 7.9 g (88% yield) of cyanomethyl N-tert-butoxycarbonylaminomethylsalicylate (m.p. 144°-146° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, CHCl 3  -d) δ1.45 (singlet, 9H, C(CH 3 ) 3 ), 4.30 (doublet, J=6 Hz, 2 H, CH 2  Ar), 5.00 (singlet, 2H, OCH 2  CN), 5.05 (triplet, J=6 H, 1H, NH), 6.83 (doublet,J=8 Hz, 1 H, ArH), 6.91 (singet, 1 H, ArH), 7.77 (doublet,J=8 Hz, 1 H, ArH), 10.12 (singlet, 1 H, OH).  13  C NMR (75 MHz, CHCl 3  -d) δ28.4, 44.3, 49.0, 80.1, 109.6, 114.2, 116.1, 118.7, 130.5, 149.7, 156.2, 162.5, 168.5. 
     Cyanomethyl Aminomethylsalicylate Hydrochloride 
     Cyanomethyl N-tert-butoxycarbonylaminomethylsalicylate (7.7 g, 26.2 mmoles)was dissolved in tetrahydrofuran (150 mL), and dry hydrogen chloride was bubbled slowly through the solution. The reaction mixture warmed as the gas dissolved. After 5 minutes, the gas was shut off, and the solution wasstirred at room temperature. A thick, creamy white precipitate formed in the solution. After 30 minutes, the reaction mixture was chilled in ice for 2 hours, then the solid was filtered, washed with diethyl ether (100 mL) and dried in vacuo over potassium hydroxide pellets to afford 5.8 g (91% yield) of cyanomethyl aminomethylsalicylate hydrochloride (m.p. darkens at 210° C., decomposes at 228° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d6) δ4.00 (singlet, 2 H, CH 2  Ar), 5.20 (singlet, 2H, OCH 2  CN), 7.05 (doublet, J=8 Hz, 1 H, ArH), 7.15 (singlet, 1 H, ArH), 7.75 (doublet, J=8 Hz, 1 H, ArH), 8.62 (broad singlet, 3H, NH 3 ), 10.38 (singlet, 1 H, OH).  13  C NMR (75 MHz, DMSO-d6) δ41.6, 49.7, 113.0, 116.1, 118.0, 119.7, 131.1, 142.3, 159.5, 166.1. 
     Cyanomethyl 4-Glutarylaminomethylsalicylate 
     Cyanomethyl 4-aminomethylsalicylate hydrochloride (1.22 g, 5.0 mmoles) was suspended in dry dichloromethane (100 mL), and the suspension was stirred in an ice/water bath. A solution of glutaric anhydride (0.57 g, 5.0 mmoles) and triethylamine (0.7 mL, 5.0 mmoles) in dry dichloromethane (25 mL) was then added dropwise over 15 minutes. The mixture was allowed to warm to room temperature, and the reaction was stirred for 18 hours. The mixture was evaporated to dryness, and the resulting solid was triturated under cold 0.1M aqueous hydrochloric acid (50 mL). The solid was collectedby filtration, washed with cold water, and dried in vacuo over potassium hydroxide pellets to afford 1.43 g (89% yield) of cyanomethyl 4-glutarylaminomethylsalicylate (m.p. 125°-126° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.75 (quintet,J=7 Hz, 2H, CH 2  CH 2  CH 2 ), 2.19 (triplet, J=7 Hz, 2H, CH 2  CONH), 2.22 (triplet, J=7 Hz, 2H, HO 2  CCH 2 ), 4.24 (doublet, J=6 Hz, 2H,ArCH 2  NH), 5.17 (singlet, 2H, OCH 2  CN), 6.81 (doublet, J=8 Hz, 1H, ArH), 6.85 (singlet, 11H, ArH), 7.70 (doublet, J=8 Hz, 11H, ArH), 8.39(triplet, J=6 Hz, 1H, NH), 10.5 (very broad singlet, 1H, OH), 11.7 (very broad singlet, 1H, CO 2  H).  13  C NMR (75 MHz, DMSO-d 6 ) δ20.7, 33.1, 34.5, 41.8, 49.7, 111.2, 115.9, 116.2, 118.5, 130.9, 149.1, 160.2, 166.6, 172.3, 174.5. 
     Cyanomethyl 4-Glutarylaminomethy salicylate Succinimidyl Ester 
     Cyanomethyl 4-glutarylaminomethylsalicylate (1.00 g, 3.1 mmoles) was dissolved in dry tetrahydrofuran (50 mL), and N-hydroxysuccinimide (0.36 g, 3.1 mmoles) and 1,3-dicyclohexylcarbodiimide (0.64 g, 3.1 mmoles) were added. The mixture was stirred at room temperature under dry nitrogen, andthe solids rapidly dissolved. After about 60 minutes, a white precipitate formed. The reaction mixture was stirred 24 hours, then chilled several hours at -20° C. The mixture was filtered cold, and the solid washed with a little tetrahydrofuran (10 mL). The combined filtrates were evaporated to dryness, and the residue was crystallized from ethyl acetate/hexanes, filtered, and dried in vacuo to afford 1.04 g (80% yield)of cyanomethyl 4-glutarylaminomethylsalicylate succinimidyl ester (m.p. 116°-119° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.88 (quintet, J=7 Hz, 2H, CH 2  CH 2  CH 2 ), 2.30 (triplet, J=7 Hz, 2H, CH 2  CONH), 2.71 (triplet, J=7 Hz, 2H, NO 2  CCH 2 ), 2.80 (singlet, 4H, COCH 2  CH 2  CO), 4.26 (doublet, J=6 Hz, 2H, ArCH 2  NH), 5.18 (singlet, 2H, OCH 2  CN), 6.82 (doublet, J=8 Hz, 1H, ArH), 6.86 (singlet, 1H, ArH), 7.71 (doublet, J=8 Hz, 1H, ArH), 8.44 (triplet,J=6 Hz,1H, NH), 10.18 (broad singlet, 1H, OH). 13C NMR (75 MHz, DMSO-d 6 ) δ20.4, 25.5, 29.7, 33.6, 41.8, 49.6, 111.2, 115.9, 116.2, 118.4, 130.9, 148.9, 160.1, 166.5, 169.0, 170.5, 171.7. 
     EXAMPLE VII 
     Preparation of a Reagent of General Formula I Cyanomethyl 4-(6-maleimidohexanoyl)aminomethylsalicylate ##STR27## 
     Cyanomethyl 4-(6-maleimidohexanoyl)aminomethylsalicylate 
     Cyanomethyl 4-aminomethylsalicylate hydrochloride (79 mg, 0.32 mmoles) and 6-maleimidocaproic acid N-hydroxysuccinimde ester (100 mg, 0.32 mmoles) were suspended in dry N,N-dimethylformamide (5.0 mL), and the suspension was stirred at room temperature. N,N-diisopropylethylamine (87 μL, 0.50mmoles) was added. The solids rapidly dissolved to afford a clear, pale yellow solution. After 30 minutes, the mixture was evaporated to dryness, and the residue was partitioned between ethyl acetate (25 mL) and cold 0.1M aqueous hydrochloric acid (25 mL). The layers were separated, and theehtyl acetate solution washed with saturated aqueous sodium bicarbonate (25mL) and saturated aqueous sodium chloride (25 mL). The ethyl acetate solution was dried over anhydrous sodium sulfate, filtered and evaporated to a white solid. The solid was crystallized from ethyl acetate/hexanes toafford 108 mg (84% yield) of cyanomethyl 4-(6-maleimidohexanoyl)aminomethylsalicylate (m.p. 141°-144° C.). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.20 (multiplet, 2H, CH 2  CH 2  CH 2  CH 2  CH 2 ), 1.53 (multiplet, 4H, CH 2  CH 2  CH 2  CH 2  CH 2 ), 2.13 (triplet, J=7 Hz, 2H, NCH 2 CH 2 ), 3.38 (triplet, J=7 Hz, 2H, CH 2  CH 2  CO), 4.26 (doublet,J=6 Hz, 2H, ArCH 2  NH), 5.02 (singlet, 2H, OCH 2  CN), 6.68 (singlet, 2H, CH═CH), 6.76 (doublet, J=8 Hz, 1H, ArH), 6.80 (singlet, 1H, ArH), 7.69 (doublet, J=8 Hz, 1H, ArH), 7.99 (triplet, J=6 Hz, 1H, NH),10.04 (singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ24.5, 25.7, 27.6, 35.2, 36.9, 41.9, 48.8, 109.1, 114.3, 115.6, 118.2, 129.8, 133.8, 149.2, 161.2, 167.6, 170.4, 172.7. 
     EXAMPLE VIII 
     Preparation of a Reagent of General Formula I 
     Cyanomethyl 4-(3-(2-Pyridyldithio)propionyl)aminomethylsalicylate ##STR28## 
     Cyanomethyl 4-(3-(2-pyridyldithio)propionyl)aminomethylsalicylate 
     Cyanomethyl 4-aminomethylsalicylate hydrochloride (79 mg, 0.32 mmoles) and 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (100 mg, 0.32mmoles) were suspended in dry N,N-dimethylformamide (5.0 mL), and the suspension was stirred at room temperature. N,N-Diisopropylethylamine (87 μL, 0.50 mmoles) was added. The solids rapidly dissolved to give a clear, pale tan solution. After 30 minutes, the mixture was evaporated to dryness, and the residue was partitioned between ethyl acetate (25 mL) andcold 0.1M aqueous hydrochloric acid (25 mL). The layers were separated and the ethyl acetate solution washed with saturated aqueous sodium bicarbonate (25 mL) and saturated aqueous sodium chloride (25 mL). The ethyl acetate solution was dried over anhydrous sodium sulfate, filtered and evaporated to a clear oil. Trituration under cold hexanes afforded 64 mg (48% yield) of a gum of cyanomethyl 4-(3-(2-pyridyldithio)propionyl)aminomethylsalicylate. 
       1  H NMR (300 MHz, DMSO-d 6 ) δ2.60 (triplet, J=7 Hz, 2H, SCH 2  CH 2 ), 3.05 (triplet, J=7 Hz, 2H, CH 2  CH 2  CO), 4.27 (doublet, J=6 Hz, 2H, ArCH 2  NH), 5.19 (singlet, 2H, OCH 2  CN), 6.84 (doublet, J=8 Hz, 1H, ArH), 6.88 (singlet, 1H, ArH), 7.23 (triplet, J=6 Hz, 1H, ArH), 7.71 (doublet, J=8 Hz, 1H, ArH), 7.74-7.82 (multiplet, 2H, ArH), 8.44 (doublet, J=6 Hz, 1H, ArH), 8.56 (triplet, J=6 Hz, 1H, NH), 10.13 (broad singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ34.0, 34.5, 41.8, 49.6, 54.9, 111.2, 115.9, 116.0, 118.4, 119.3, 121.3, 130.8, 137.9, 148.6, 149.7, 159.4, 160.0, 166.4, 170.3. 
     EXAMPLE IX 
     Preparation of a Reagent of General Formula III 4-Glutarylaminomethylsalicylhydroxamic Acid Hydrazide ##STR29## 
     Methyl 4-Glutarylaminomethylsalicylate N-tert-Butyloxycarbonylhydrazide 
     Methyl 4-glutarylaminomethylsalicylate succinimidyl ester (2.57 g, 6.8 mmoles)  prepared as for the succinyl derivative above, substituting glutaric anhydride for succinic anhydride! was dissolved in dry tetrahydrofuran (100 mL), and tert-butylcarbazate (0.90 g, 6.8 mmoles) wasadded. The reaction was stirred at room temperature for 60 hours. The solution was then evaporated to dryness, and the residue dissolved in ethyl acetate (100 mL). The ethyl acetate solution was washed with saturated aqueous potassium bicarbonate (100 mL) and saturated aqueous sodium chloride (100 mL). It was then dried over anhydrous sodium sulfate,filtered, and evaporated to dryness. The residue was crystallized by dissolving it in ethyl acetate (50 mL) with gentle warming, adding hexanes(50 L) to the warm solution, and chilling at -20° C. Once crystallization began, another portion of hexanes (50 mL) was added, and the mixture was chilled for 18 hours at -20° C. Finally, the solid was filtered, washed with hexanes:ethyl acetate (2:1  v/v!, 90 mL), and dried in vacuo to afford 2.51 g (90% yield) of methyl 4-glutarylaminomethyl-salicylate N-tert-butyloxycarbonylhydrazide (m.p. 68°-72° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.37 (singlet, 9H, (CH 3 ) 3  C), 1.74 (quintet, J=7 Hz, 2H, CH 2  CH 2  CH 2 ), 2.07 (triplet, J=7 Hz, 2H, CH 2  CONHCH 2 ), 2.17 (triplet, J=7 Hz, 2H, HNHNOCCH 2 ), 3.86 (singlet, 3H, OCH 3 ), 4.23(doublet, J=6 Hz, 2H, ArCH 2  NH), 6.80 (doublet, J=8 Hz, 1H, ArH), 6.82(singlet, 1H, ArH), 7.71 (doublet, J=8 Hz, 1H, ArH), 8.37 (triplet, J=6 Hz,1H, NH), 8.65 (singlet, 1H, NHNHCOCH 2 ), 9.48 (singslet, 1H, OCONHNH), 10.50 (singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ21.2,28.0, 32.6, 34.6, 41.7, 52.4, 79.1, 111.4, 115.5, 118.3, 130.2, 148.5, 155.5, 160.4, 169.4, 171.7, 172.1. 
     4-Glutarylaminomethylsalicylhydroxamic Acid N-tert-Butyloxycarbonylhydrazide 
     Methyl 4-glutarylaminomethylsalicylate N-tert-butyloxycarbonylhydrazide (2.00 g, 4.9 mmoles) was added to a cooled (ice/water bath) solution of hydroxylamine sulfate (0.82 g, 5.0 mmoles), sodium hydroxide (1.00 g, 25.0mmoles) and sodium sulfite (0.20 g, mmoles) in water (25 mL). The suspension was stirred for 18 hours in the dark, allowing it to warm to room temperature. The solution was then filtered to remove some insoluble material, and the pale yellow solution was chilled in an ice/water bath. The cold reaction mixture was slowly titrated to pH 3-4 with cold sulfuricacid (25%  v/v! aqueous), during which time a gummy material precipitated. The mixture was then chilled several hours in ice, and the liquid was decanted. The remaining amorphous solid was dissolved in methanol (25 mL),filtered, and evaporated to a white foam. The foam was dried in vacuo to 1.86 g (94% yield) of crude 4-glutarylaminomethyl-salicylhydroxamic acid N-tert-butyloxycarbonylhydrazide. 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.37 (singlet, 9H, (CH 3 ) 3  C), 1.74 (quintet, J=7 Hz, 2H, CH 2  CH 2  CH 2 ), 2.10 (triplet, J=7 Hz, 2H, CH 2  CONHCH 2 ), 2.16 (triplet, J=7 Hz, 2H, HNHNOCCH 2 ), 4.20 (doublet, J=6 Hz, 2H, ArCH 2  NH), 6.71 (doublet, J=8 Hz, 1H, ArH), 6.74 (singslet, 1H, ArH),7.60 (doublet, J=8 Hz, 1H, ArH), 8.32 (triplet, J=6 Hz, 1H, NH), 8.65 (singlet, 1H, NHNHCOCH 2 ), 9.29 (broad singlet, 1H, NHOH), 9.48 (singlet, 1H, OCONHNH), 11.37 (broad singlet, 1H, ArOH), 12.32 (broad singlet, 1H, NHOH).  13  C NMR (75 MHz, DMSO-d 6 ) δ21.2, 28.0, 32.6, 34.6, 41.7, 79.1, 112.4, 115.6, 117.6, 127.1, 145.9, 155.6, 159.8, 166.5, 171.7, 172.1. 
     4-Glutarylaminomethylsalicylhydroxamic Acid Hydrazide Hydrochloride 
     4-Glutarylaminomethylsalicylhydroxamic acid N-tert-butyloxycarbonylhydrazide (1.86 g, 4.6 mmoles) was dissolved in drytetrahydrofuran (100 mL), and dry hydrogen chloride was bubbled slowly through the solution. The reaction mixture warmed as the gas dissolved. After 5 minutes, the gas was shut off, and the solution was stirred at room temperature. A white precipitate formed in the solution. After 30 minutes, the reaction mixture was chilled in ice for 2 hours, then the solid was filtered, washed with diethyl ether (50 mL) and dried in vacuo over potassium hydroxide pellets to afford 1.51 g (95% yield) of 4-glutarylaminomethyl-salicylhydroxamic acid hydrazide hydrochloride (m.p.shrinks at 65° C., decomposes with effervescence at 100° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.72 (quintet,J=7 Hz, 2H, CH 2  CH 2  CH 2 ), 2.17 (triplet, J=7 Hz, 2H, CH 2  CONHCH 2 ), 2.23 (triplet, J=7 Hz, 2H, H 3  NHNOCCH 2 ), 4.17 (doublet, J=6 Hz, 2H, ArCH 2  NH), 6.69 (doublet, J=8 Hz, 1H, ArH), 6.74 (singlet, 1H, ArH), 7.66 (doublet, J=8 Hz, 1H, ArH), 8.49 (triplet, J=6 Hz, 1H, NH), 10.49 (broad singlet, 4H, NH 3  NHCOCH 2 ), 11.08 (broad singlet, 1H, ArOH), 11.43 (broad singlet, 2H, NHOH).  13  C NMR (75 MHz, DMSO-d 6 ) δ21.0, 25.2, 32.3, 34.4, 41.8, 67.2, 112.6, 115.7, 117.7, 127.4, 145.9, 159.8, 166.5, 171.7, 172.1. 
     EXAMPLE X 
     Preparation Of A Reagent Of General Formula III 4-Glutarylaminomethylsalicyl(O-methyl)hydroxamic Acid Succinimidyl Ester ##STR30## 
     Methyl N-(Benzyloxycarbonyl)-4-aminomethylsalicylat 
     Methyl 4-aminomethylsalicylate hydrochloride (5.04 g, 23.2 mmoles) was suspended in chloroform (80 mL) and N,N-diisopropylethylamine (4.10 mL, 23.5 mmoles) and N-(benzyl-oxycarbonyloxy)succinimide (6.48 g, 26.0 mmoles) were added. The reaction mixture was stirred at room temperature for 4 hours, during which time all solids dissolved. The reaction mixture was then extracted with 1N aqueous hydrochloric acid (100 mL), water (75 mL), and saturated aqueous sodium chloride (50 mL). The chloroform solution was dried over anhydrous magnesium sulfate, filtered and evaporated to dryness to give a white solid. The product was crystallized from ethyl acetate:hexanes to afford 6.17 g (84% yield) of methyl N-(benzyloxy-carbonyl)-4-aminomethylsalicylate (m.p. 91°-92°C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ3.86 (singlet, 3H, OCH 3 ), 4.21 (doublet, J=6 Hz, 2H, NHCH 2 ), 5.05 (singlet, 2H, CH 2  O), 6.82 (doublet, J=8 Hz, 1H, ArH), 6.86 (singlet, 1H, ArH), 7.27-7.36 (multiplet, 5H, ArH), 7.72 (doublet, J=8 Hz, 1H, ArH), 7.90 (triplet, J=6 Hz, 1H, NH), 10.53 (singlet, 1H, OH).  13  C NMR (75 MHz, DMSO-d 6 ) δ43.5, 52.4, 65.6, 111.5, 115.4, 118.2, 127.9, 128.0, 128.5, 130.2, 137.3, 148.6, 156.7, 160.5, 169.5. 
     Methyl N-(Benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicylate 
     Methyl N-(benzyloxycarbonyl)-4-aminomethylsalicylate (6.06 g, 19.2 mmoles) was dissolved in acetone (150 mL), and benzyl bromide (2.60 mL, 21.9 mmoles) and anhydrous potassium carbonate (13.28 g, 96.1 mmoles) were added. The mixture was stirred and heated at reflux for 22 hours. The mixture was concentrated to remove most of the acetone, and ethyl acetate (100 mL) was added. Aqueous hydrochloric acid (1N, 200 mL) was added slowly, swirling frequently to dissolve the solid carbonate. The layers were separated, and the aqueous layer was extracted with ethyl acetate (50mL). The ethyl acetate solutions were combined and washed with water (100 mL) and saturated aqueous sodium chloride (50 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated to about 50 mL. This solution was heated to boiling, and hexanes (150 mL) were added. The solution was cooled in ice, and crystals slowly formed. The crystals were collected by filtration, washed with hexanes, and dried in vacuo to afford6.97 g (89% yield) of methyl N-(benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicylate (m.p. 106°-107° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ3.78 (singlet, 3H, OCH 3 ), 4.27 (doublet, J 6 Hz, 2H, NHCH 2 ), 5.07 (singlet, 2H, CH 2  OCO), 5.15 (singlet, 2H, CH 2  O), 6.94 (doublet, J=8 Hz, 1H, ArH), 7.16 (singlet, 1H, ArH), 7.26-7.42 (multiplet, 8H, ArH), 7.49-7.51 (multiplet, 2H, ArH), 7.68 (doublet, J=8 Hz, 1H, ArH), 7.90 (triplet, J=6 Hz, 1H, NH).  13  C NMR (75 MHz, DMSO-d 6 ) δ43.8, 51.8, 65.6, 69.7, 112.5,112.6, 118.9, 119.0, 127.2, 127.9, 128.0, 128.5 (2 carbons), 131.3, 137.0, 137.3, 146.2, 156.7, 157.8, 166.1. 
     N-(Benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicylic acid 
     Methyl N-(benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicylate (6.81 g 16.8 mmoles) was dissolved in tetrahydrofuran (50 mL). A solution of lithium hydroxide monohydrate (0.78 g 18.5 mmoles) in water (25 mL) was added, and the reaction mixture was stirred and heated at 75° C. for 24 hours. The solution was cooled, and 1N aqueous hydrochloric acid (50 mL) was added. The reaction mixture was extracted twice with ethyl acetate (150 mL then 50 mL). The combined ethyl acetate extracts were washed with water (75 mL) and saturated aqueous sodium chloride (50 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated to 150 mL. The ethyl acetate solution was heated to boiling, and hexanes (150 mL)was added. The solution was cooled in ice, and crystals formed. The crystals were collected by filtration, washed with hexanes, and dried in vacuo to afford 5.92 g (90% yield) of N-(benzyloxy-carbonyl)-4-aminomethyl-2-O-benzylsalicylic acid (m.p. 139°-140° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ4.23 (doublet, J=6 Hz, 2H, NHCH 2 ), 5.06 (singlet, 2H, CH 2  OCO), 5.13 (singlet, 2H, CH 2 O), 6.90 (doublet, J=8 Hz, 1H, ArH), 7.11 (singlet, 1H, ArH), 7.28-7.40 (multiplet, 8H, ArH), 7.48-7.51 (multiplet, 2H, ArH), 7.64 (doublet, J=8 Hz, 1H, ArH), 7.88 (triplet, J=6 Hz, 1H, NH), 12.57 (singet, 1H, COOH).  13  C NMR (75 MHz, DMSO-d 6 ) δ43.8, 65.6, 69.8, 112.5(2 carbons), 118.9, 120.4, 127.4, 127.9, 128.0, 128.5 (2 carbons), 131.2, 137.1, 137.4, 145.6, 156.7, 157.5, 167.4. 
     N-(Benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicyl(O-methyl)hydroxamic acid 
     N-(Benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicylic acid (3.07 g, 7.84 mmoles) was dissolved in anhydrous N,N-dimethylformamide (75 mL) under drynitrogen. After cooling the solution to in an ice/water bath, triethylamine(2.20 mL, 15.8 mmoles) was added followed by isobutyl chloroformate (1.10 mL, 8.48 mmoles). The reaction was stirred in ice for 1.5 hours. Methoxylamine hydrochloride (0.67 g, 8.02 mmoles) was added and the reaction mixture was allowed to warm to room temperature. After 3 hours, the reaction was diluted with ethyl acetate (150 mL) and extracted with 1Naqueous hydrochloric acid (100 mL), water (100 mL), and saturated aqueous sodium chloride (50 mL). The ethyl acetate solution was dried over anhydrous magnesium sulfate, filtered and concentrated to about 50 mL. This solution was boiled and hexanes (100 mL) were added. Upon cooling in an ice bath, crystals formed quickly. They were collected by filtration and washed with hexane to afford 2.87 g (87% yield) of N-(benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicyl(O-methyl)hydroxamic acid (m.p. 116°-117° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ3.63 (singlet, 3H, NHOCH 3 ), 4.20 (doublet, J=6 Hz, 2H, NHCH 2 ), 5.04 (singlet, 2H, CH 2  OCO), 5.13 (singlet, 2H, CH 2  O), 6.90 (doublet, J=8 Hz, 1H, ArH), 7.08 (singlet, 1H, ArH), 7.29-7.49 (multiplet, 1 1H, ArH), 7.88 (triplet, J=6 Hz, 1H, NH), 11.13 (singlet, 1H, NHOCH 3 ).  13  C NMR(75 MHz, DMSO-d 6 ) δ43.7, 63.2, 65.5, 69.8, 111.8, 119.2, 121.7, 127.6, 127.9, 128.0, 128.1, 128.5 (2 carbons), 129.8, 136.8, 137.3, 144.3,155.9, 156.6, 163.3. 
     4-Aminomethylsalicyl(O-methyl)hydroxamic Acid Hydrochloride 
     N-(Benzyloxycarbonyl)-4-aminomethyl-2-O-benzylsalicyl(O-methyl)hydroxamic acid (1.42 g, 3.38 mmoles) and palladium on carbon (0.10 g, 10%  w/w!) were placed in a 1L hydrogenation flask under dry nitrogen. Ethanol (150 mL) was added, followed by concentrated aqueous hydrochloric acid (0.30 mL). The flask was affixed to the Parr hydrogenator and shaken under 35 psi of hydrogen for 7 hours at room temperature. The reaction mixture was then filtered through Celite to remove the catalyst, and the filtrate was concentrated until a precipitate began to form. The mixture was cooled in ice, the solid collected by filtration, washed with hexanes, and dried in vacuo to afford 0.65 g (83% yield) of 4-aminomethyl-salicyl(O-methyl)-hydroxamic acid hydrochloride (m.p. &gt;250° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ3.71 (singlet, 3H, NHOCH 3 ), 3.95 (singlet, 2H, NH 3  CH 2 ), 7.00 (doublet, J=8 Hz, 1H, ArH), 7.05 (singlet, 1H, ArH), 7.72 (doublet, J=8 Hz, H, ArH), 8.53 (broad singlet, 3H, NH3), 12.01 (broad singlet, 2H, NHOCH 3  and OH).  13  C NMR (75 MHz, DMSO-d6) δ41.7, 63.6, 114.2, 117.6, 119.3, 128.2, 140.0, 159.4, 166.1. 
     4-Glutarylaminomethylsalicyl(O-methyl)hydroxamic Acid Succinimidyl Ester 
     4-Aminomethylsalicyl(O-methyl)hydroxamic acid hydrochloride (0.57 g 1,82 mmoles) was dissolved in anhydrous N,N-dimethylformamide (10 mL), and N,N-diisopropylethyl amine (0.35 mL, 2.01 mmoles) and glutaric anhydride (0.23 g, 2.02 mmoles) were added. The mixture was stirred at room temperature for 26 hours, and then N-hydroxysuccinimide (0.23 g, 2.00 mmoles) and 1,3-dicyclohexylcarbodiimide (0.42 g, 2.01 mmoles) were added.The reaction mixture was stirred for an additional 24 hours at room temperature. The mixture was then filtered and diluted with ethyl acetate (100 mL). The ethyl acetate solution was washed with 1N aqueous hydrochloric acid (100 mL), water (100 mL), and saturated aqueous sodium chloride (50 mL). The solution was then dried over anhydrous magnesium sulfate, filtered, and evaporated to dryness. The crude gummy product was triturated under a mixture of ethyl acetate and diethyl ether to produce asolid which was filtered and dried in vacuo to afford 0.15 g (20% yield) of4-glutarylaminomethylsalicyl(O-methyl)hydroxamic acid succinimidyl ester (m.p. 121°-124° C., open capillary, uncorrected). 
       1  H NMR (300 MHz, DMSO-d 6 ) δ1.85 (quintet, J=7 Hz, 2H, CH 2  CH 2  CH 2 ), 2.27 (triplet, J=7 Hz, 2H, CH 2  CONHCH 2 ), 2.69 (triplet, J=7 Hz, 2H, NOCCH 2 ), 2.80 (singlet, 4H,COCH 2  CH 2  CO), 3.70 (singlet, 3H, NHOCH 3 ), 4.21 (doublet, J=6 Hz, 2H, NHCH 2 ), 6.75 (doublet, J=8 Hz, 1H, ArH), 6.77 (singlet, 1H, ArH), 7.57 (doublet, J=8 Hz, H, ArH), 8.39 (triplet, J=6 Hz, 1H, NHCH 2 ), 11.75 (singlet, 1H, OH), 11.78 (singlet, 1H, NHOCH 3 ).  13  C NMR (75 MHz, DMSO-d6) δ20.4, 25.4, 29.7, 33.4, 33.6, 41.7,112.8, 115.6, 117.8, 127.8, 146.2, 159.3, 166.3, 169.0, 170.5, 171.5. 
     EXAMPLE XI 
     Preparation Of A Reagent Of General Formula III 4-(3-(2-Pyridyldithio)propionyl)aminomethylsalicyl(O-methyl)hydroxamic Acid ##STR31## 
     4-(3-(2-Pyridyldithio)propionyl)aminomethylsalicyl(O-methyl)hydroxamic Acid 
     3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (100 mg, 0.32 mmole) was dissolved in dry N,N-dimethylformamide (5.0 mL) and NN-diisopropylethylamine (65 μL, 0.36 mmole) was added followed by 4-aminomethylsalicyl(O-methyl)hydroxamic acid hydrochloride (82 mg, 0.35 mmole). The reaction was stirred for 6 hours at room temperature. The solvent was evaporated in vacuo, and the residue was chromatographed on silica gel eluting with dichloromethane/methanol/acetic acid (95:5:1  v/v/v!). Fractions containing the desired product were pooled and evaporated to an oil to afford 44 n (35% yield) of 4-(3-(2-pyridyldithio)-propionyl)aminomethylsalicyl(O-methyl)hydroxamic acid. 
       1  H NMR (300 MHz,DMSO-d 6 ) δ2.58 (triplet, J=7 Hz, 2H, CH 2  CH 2  S), 3.04 (triplet, J=7 Hz, 2H, COCH 2  CH 2 ), 3.70 (singlet, 3H, OCH 3 ), 4.23 (doublet, J=6 Hz, 2H, ArCH 2  NH), 6.76 (doublet,J=8 Hz, 1H, ArH), 6.79 (singlet, 1H, ArH), 7.23 (triplet, J=6 Hz, 1H, ArH), 7.57 (doublet, J=8 Hz, 1 H, ArH), 7.73-7.82 (multiplet, 2H, ArH), 8.44 (doublet, J=6 Hz, 1H, ArH), 8.48 (triplet, J=6 Hz, 1H, NH),11.80 (broad singlet, 2H, OH and NHO).  13  C NMR (75 MHz, DMSO-d 6 )67 34.1, 34.5, 41.8, 63.5, 112.7, 115.7, 117.8, 119.3, 121.3,127.7, 138.0, 145.9, 149.8, 159.4, 166.3, 170.2, 172.8. 
     EXAMPLE XII 
     Preparation of Conjugates of General Formula IV 
     Synthesis of 5&#39;-PBA-labeled Oligodeoxyribonucleotide Conjugates 
     Oligodeoxyribonucleotide 7172 (sequence 5&#39;-GATTACGCCAGTTGTACGGAC-3&#39;) was synthesized on a 1 μmole scale using standard automated phosphoramiditechemistry (Beckman Instruments Oligo 1000 and associated reagents). A protected amine-containing phosphor-amidite (Aminolink 2, Applied Biosystems or UniLink Amino Modifier, Clontech) was employed on the same instrument to introduce one to four, reactive primary amines onto the 5&#39;-end of the oligodeoxyribonucleotide using standard chemistry. The completed oligodeoxyribonucleotide was then cleaved from the support and the nucleobases deprotected using an UltraFast Deprotection kit (Beckman Instruments) and the protocol supplied by the manufacturer. 
     The amino-oligonucleotides were purified by ethanol precipitation, dissolved in 0.8 mL of 0.1M NaHCO 3 , and condensed with phenylboronic acid reagent (PBA-Z-NHS) having a reactive N-hydroxysuccinimidyl ester moiety (5 mgs per mmole of primary amino groups on the amino-oligonucleotide in 0.2 mL of anhydrous N,N-dimethylformamide) for 2-18 hours at room temperature. 
     The crude PBA-modified oligonucleotide was isolated from the reaction mixture by gel filtration on a KwikSep Dextran column (Pierce Chemical) in0.1M aqueous triethylammonium acetate, pH 6.5. The PBA-modified oligonucleotide was then concentrated in a vacuum centrifuge to 1 mL, and purified by reverse phase HPLC on a 4.6 mm×250 mm C18 column, with atriethylammonium acetate-acetonitrile gradient. The desired product peak was collected and evaporated to a small pellet in a vacuum centrifuge, dissolved in 0.5 mL of water, and stored frozen. 
     Preparation of Salicylhydroxamic Acid Magnetic Beads 
     Ten milliliters of unmodified M280 or M450 magnetic beads (Dynal) were gradually dehydrated into acetonitrile, and converted to aldehyde modifiedbeads using oxalyl chloride activated N,N-dimethylsulfoxide and triethylamine in dichloromethane at -78° C. The resulting aldehyde bearing beads were gradually rehydrated and suspended in 5 mL of 0.1M sodium acetate pH 5.5. The aldehyde groups were coupled with 4-glutarylaminomethylsalicylhydroxamic acid hydrazide (SHA-Z-NHNH 2 ) by adding 15-25 mgs dissolved in 200 uL N,N-dimethylsulfoxide, and rotating coupling reaction over night at room temperature. The beads were then washed extensively with water and stored in 5 mL of 10% ethanol. 
     Preparation of Salicylhydroxamic Acid (SHA) Sepharose 4B 
     SHA-Sepharose 4B was prepared by mixing 130 mg of (6-aminohexanoyl)-4-amino-methylsalicylhydroxamic acid (SHA-Z-NH 2 ), dissolved in 30 mL 0.2M NaHCO 3 , with. 6.5 g HCl washed CNBr activatedSepharose 4B (Pharmacia) overnight at room temperature. After the coupling reaction, 2 mL 0.5M Tris, pH 8.5 were added and the gel slurry mixed at room temperature for 1 hour, and washed with water, 0.5M NaCl, and water again. The resulting SHA-Sepharose 4B was suspended in 30 mL of 20% ethanol, and stored at 4° C. 
     Preparation of a Phenylboronic Acid-α-Biotin Antibody Conjugate 
     One milliliter of α-Biotin monoclonal IgG 1  antibody (6.5 mg in 0.1 M NaHCO 3 ) was conjugated with 440 nmoles of PBA-Z-NHS (7.4 ul of 60 mM PBA-Z-NHS dissolved in N,N-dimethylsulfoxide) for 1 hour at room temperature. Unconjugated PBA-Z-NHS and its hydrolysis products were removed by dialysis. The ultra-violet absorbance spectrum of the resultingconjugate (PBA-α-Biotin) exhibited an increase in A 260  relative to A 280  consistent with phenylboronic acid modification. 
     Preparation of a Phenylboronic Acid-Alkaline Phosphatase Conjugate 
     One milliliter of alkaline phosphatase (Sigma, 6 mg/mL) was dialyzed against one liter of 0.1M NaHCO 3 , and conjugated with 700 nmoles of PBA-Z-NHS (10 uL of 70 mM stock in N,N-dimethylformamide) for two hours onice. Unconjugated PBA-Z-NHS and its hydrolysis products were removed by dialysis in 0.1M NaHCO 3 . The ultra-violet absorbance spectrum of the resulting conjugate (PBA-AP) exhibited an increase in A 260  relative to A 280  consistent with phenylboronic acid modification. The conjugate was stored at 4° C. 
     Preparation of Salicylhydroxamic Acid-Goat α-Mouse Antibody Conjugate 
     Two milliliters of goat α-mouse antibody (Rockland, 8.8 mg/mL in 0.1MNaHCO 3 ) were conjugated with 2.35 umoles of methyl 4-glutarylaminomethylsalicylate succinimidyl ester  SA(OMe)-Z-NHS! for 1 hour at room temperature. The methyl ester of the conjugate was converted to a hydroxamic acid by adding two milliliters of 2M NH 2  OH, pH 10, adjusting the pH to 10 with 1N NaOH, and incubating the reaction at room temperature for three days. NH 2  OH and unconjugated SA(OMe)-X-NHS andits hydrolysis products were removed by gel filtration on a G-25 Sephadex column (Pharmacia) in 0.1M NaHCO 3 , and the conjugate (SHA-goat α-mouse) was stored at 4° C. 
     Polymerase Chain Reaction (PCR) Protocol 
     A region of Lambda DNA (801 bp) was amplified by the polymerase chain reaction. The PCR reaction contained 200 uM dATP, dCTP, dGTP, and dTTP in addition to Biotin- and PBA- modified oligonucelotide primers at 1 uM in 1X PCR Buffer II (Perkin Elmer), Lambda DNA (1 ng/uL), and 1U of Thermus aquaticus DNA polymerase. The reaction mixture was denatured at 92°C. for one minute and amplified by 35 cycles of PCR at 95° C. for 10seconds, 62° C. for 20 seconds, and 72° C. for 30 seconds, with a final extension at 72° C. for 5 minutes. The reaction produced 50-100 ng of amplified product (801 bp), which exhibited retardedmobility relative to unmodified PCR product during electrophoresis on 1% agarose gels in 50 mM Tris, 100 mM borate, 2 mM EDTA, pH 8.3. 
     EXAMPLE XIII 
     Preparation of Bioconjugates of General Formula VI Detection of PBA-LabeledPCR Product 
     Detection of PBA-Labeled PCR Product on SHA-Magnetic Beads 
     PBA-labeled PCR product (0.02 μL-5 μL) was diluted into 25-100 μL of 1.5M NaCl, 150 mM sodium citrate, pH 7 (10X SSC), and added to a polypropylene microtiter plate well containing SHA-magnetic particles (10-50 ul). The particles and PCR product were mixed occasionally for 30-60 minutes at room temperature. The magnetic particles were captured inthe bottom of the wells with a magnetic plate and washed five times in 150 mM NaCl, 20 mM Tris-HCl, 0.02% Tween 20, pH 8 (ELISA Wash Buffer). One hundred microliters of streptavidin alkaline-phosphatase (Boehringher Mannheim, 0.2 U/mL in 1 mg/mL BSA, NaCl, Tris-HCl, pH 8) were added and mixed with the magnetic particles for 30 minutes at room temperature. The magnetic particles were captured in the bottom of the wells with a magnetic plate and washed 5 times with ELISA Wash. Alkaline phosphatase substrate was added (1 mg/ml p-nitrophenyl phosphate in 1M diethanolamine buffer, 1 mM MgCl 2 , 0.1 mM ZnCl 2 , pH 10.4), and the color developed at 37° C. for 10-60 minutes. The lower limit of detectionwas 50 pg of PCR product. 
     EXAMPLE XIV 
     Preparation of Bioconjugates of General Formula VI Detection of a PBA-Labeled Oligonucleotide Hybrid 
     Detection of a PBA-Labeled Oligonucleotide Hybridized to a Biotin-Labeled Oligonucleotide 
     A 42-mer oligonucleotide was hybridized with two 21-mer oligonucleotides bearing 5&#39;-PBA and Biotin labels in 1.5M NaCl, 150 mM sodium citrate, pH 7, at 45 C for ten minutes. Twenty-five microliters of the hybridization mixture was mixed with 1-50 uL of SHA-magnetic particles (Dynal, M450) in a polypropylene microtiter plate well. After 30 minutes, the magnetic particles were captured in the bottom of the well with a magnetic plate, and washed five times with 150 mM NaCl, 20 mM Tris-HCl, 0.02% Tween 20, pH 
     One hundred microliters of SHA-AP (1 ug/mL in 1 mg/mL BSA, 140 mM NaCl, 10 mM Tris-HCl, pH 8) were added to the magnetic particles and mixed well. After 30 minutes, the magnetic particles were captured in the bottom of the well with a magnetic plate, and washed six times with 150 mM NaCl, 20 mM Tris-HCl, 0.02% Tween 20, pH 8. The particles were mixed with alkaline phosphatse substrate (1 mg/mL p-nitrophenyl phosphate in 1M diethanolaminebuffer, 1 mM MgCl 2 , 0.1 mM ZnCl 2 , pH 10.4) and incubated at 37° C. for 90 minutes. The A 405  was measured with a ELISA plate reader (Molecular Devices). As little as 45 pg of oligonucleotide 42-mer was detected. Experimental controls lacking either the 42-mer, or the PBA or Biotin labeled oligonucleotides did not produce a significant A 405 . 
     EXAMPLE XV 
     Preparation of Bioconjugates of General Formula VI 
     Immobilization of a PBA-α-Biotin Conjugate on SHA-Sepharose 4B 
     One mg of PBA-α-Biotin, diluted to 1 mL with Tris buffered saline, was applied to small column of SHA-Sepharose 4B (1.0×2.0 cm), and washed extensively with Tris buffered saline. The size of the A 280  peak of the material not binding to the column indicated that almost all of the PBA-conjugate was immobilized on the column. 
     Biotin binding activity of the column was assayed by applying to the column5 mL of 1 ug/mL biotinylated alkaline phosphatase in Tris buffered saline containing 5 mg/mL bovine serum albumin (BSA). A sample of the peak of thematerial flowing through the column was collected for comparison of the enzymatic activity with a sample of the alkaline phosphatase dilution applied to column. After applying the sample, the column was washed with 20 mL of buffer. After washing, a very small sample of column material (25uL liquid containing about 1 uL gel) was collected to measure the enzymaticactivity bound to the gel as a result of capture by the immobilized α-biotin antibody. 
     The alkaline phosphatase activity was measured by incubating 25 uL of the enzyme samples in 250 uL of 1 mg/mL p-nitrophenylphosphate in 1M diethanolamine buffer, 1 mM MgCl 2 , and 0.1 mM ZnCl 2 , pH 10.4, for 20 minutes and then adding 650 uL of 0.1M NaHCO 3 , 10 mM EDTA. Relative to a buffer blank, the A 405  of the sample applied to the column was 1.57, while the A 405  of the peak of the material not retained by the column was only 0.042, indicating that virtually all the enzyme conjugate was captured by the column. The small amount of gel assayed produced an A 405  of 1.30, demonstrating that the enzyme was in fact captured by the column. 
     EXAMPLE XVI 
     Preparation of Bioconjugates of General Formula VI 
     Immobilization of a PBA-Alkaline Phosphatase Conjugate on SHA-Magnetic Beads 
     PBA-conjugated alkaline phosphatase was diluted to 5 ug/mL in Tris bufferedsaline containing 5 mg/mL bovine serum albumin. Two hundred microliters of diluted PBA-conjugated enzyme were mixed with 5, 10, or 20 uL of SHA-magnetic beads (Dynal, M280). The enzyme was also mixed with 40 uL of unmodified beads as a control. The beads were mixed gently for 10 minutes on ice, after which the beads were captured with a rare earth magnet and washed 4 times with Tris buffered saline. The beads were then suspended in250 uL of 1 mg/mL p-nitrophenylphosphate in 1 M diethanolamine buffer, 1 mMMgCl 2 , and 0.1 mM ZnCl 2 , pH 10.4, and mixed occasionally at 37° C. for 10 minutes. The reactions were terminated with 750 uL ofTris buffered saline, 5 mM EDTA. The A 405  relative to a buffer blank was measured to determine the alkaline phosphatase activity bound to the magnetic beads. The control beads produced an A 405  of only 0.15, while the SHA-magnetic beads produced an A 405  of 0.62, 0.97, and 1.33for 5, 10, and 20 uL of beads, respectively, indicating the immobilization of significant amounts of PBA-AP conjugate on the surface of the beads.