Abstract:
Broadly this invention provides inbred corn line ZS01301. The methods for producing a corn plant by crossing the inbred line ZS01301 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01301 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01301 with at least one other corn line.

Description:
FIELD OF THE INVENTION 
     This invention is in the field of corn breeding, specifically relating to an inbred corn line designated ZS01301. 
     BACKGROUND OF THE INVENTION 
     The original maize plant was indigenous to the Western Hemisphere. The plants were weedlike and only through the efforts of early breeders was a cultivated crop species developed. The physical traits of maize are such that self pollination or cross pollination can occur. Each plant has a separate male and female flower, the tassel and ear, respectively. Natural pollination occurs when wind transfers pollen from tassel to the silks on the corn ears. This type of pollination contributed to the wide variation of maize varieties present in the Western Hemisphere. 
     The development of a planned breeding program for maize only occurred in the last century. Originally, maize was an open pollinated variety having heterogeneous genotypes. The maize farmer selected uniform ears from the yield of these genotypes and reserved them for planting the next season. The result was a field of maize plants that were segregating for a variety of traits. This type of maize selection lead to at most incremental increases in seed yield. 
     Large increases in seed yield were the result of the development of hybrid corn varieties in planned breeding programs. Hybrids were developed by selecting corn lines and selfing these lines for several generations to develop homozygous pure inbred lines and crossing selected inbred lines with unrelated inbred lines to produce hybrid progeny (F1). Inbred lines can be difficult to produce since the inbreeding process in corn decreases the vigor. However, when two inbred lines are crossed, the hybrid plant evidences greatly increased vigor compared to open pollinated segregating maize plants. An important factor of the homozygosity and the homogeneity of the inbred lines is that the hybrid from any cross will always be the same, and can be reproduced. 
     The ultimate objective of the commercial maize seed companies is to produce high yielding, agronomically sound plants which perform well in certain regions or areas of the Corn Belt. To produce these types of hybrids, the companies must develop inbreds which carry needed traits into the hybrid combination. Hybrids are not uniformly adapted for the Corn Belt, but are specifically adapted for regions of the Corn Belt. Northern regions of the Corn Belt require shorter season hybrids than do southern regions of the Corn Belt. Hybrids that grow well in Colorado and Nebraska soils may not flourish in rich Illinois soil. Thus, a variety of major agronomic traits are important in hybrid combination for the various Corn Belt regions, and have an impact on hybrid performance. 
     Inbred line development and hybrid testing have been emphasized in the past half century in commercial maize production as a means to increase hybrid performance. Inbred development is usually done by pedigree selection. Pedigree selection can be selection in an F 2  population produced from a planned cross of two genotypes (often elite inbred lines), or selection of progeny of synthetic varieties, open pollinated, composite, or backcross populations. This type of selection is effective for highly inheritable traits, but other traits, for example, yield requires replicated test crosses at a variety of stages for accurate selection. 
     Maize breeders select for a variety of traits in inbreds that impact hybrid performance along with selecting for acceptable parental traits. Such traits include yield potential in hybrid combination; dry down; maturity; grain moisture at harvest; greensnap; resistance to root lodging; resistance to stalk lodging; grain quality; disease and insect resistance; ear and plant height; performance in different soil types such as: low level of organic matter, clay, sand, black, high pH, low pH; performance in: wet environments, drought environments, and no tillage conditions. These traits appear to be governed by a complex genetic system that makes selection and breeding of an inbred line extremely difficult. Even if an inbred in hybrid combination has excellent yield (a desired characteristic), it may not be useful because it fails to have acceptable parental traits such as seed yield, seed size, pollen production, good silks, plant height, etc. 
     To illustrate the difficulty of breeding and developing inbred lines, the following example is given. Two inbreds compared for similarity of 29 traits differed significantly for 18 traits between the two lines. If 18 simply inherited single gene traits were polymorphic with gene frequencies of 0.5 in the parental lines, and assuming independent segregation (as would essentially be the case if each trait resided on a different chromosome arm), then the specific combination of these traits as embodied in an inbred would only be expected to become fixed at a rate of one in 262,144 possible homozygous genetic combinations. Selection of the specific inbred combination is also influenced by the specific selection environment on many of these 18 traits which makes the probability of obtaining this one inbred even more remote. Thus, the general procedure of producing a non segregating F 1  generation and self pollinating to produce a F 2  generation that segregates for traits does not easily lead to a useful inbred. Great care and breeder expertise must be used in selection of breeding material to continue to increase yield and agronomics of inbreds and resultant commercial hybrids. 
     SUMMARY OF THE INVENTION 
     The present invention relates to an inbred corn line ZS01301. Specifically, this invention relates to plants and seeds of this line. Additionally, this relates to a method of producing hybrid seed corn from this inbred. More particularly, this invention relates to the unique combination of traits that combine in corn line ZS01301. 
     Generally then, broadly the present invention includes an inbred corn seed designated ZS01301. This seed produces a corn plant. 
     The invention also includes the tissue culture of regenerable cells of ZS01301 wherein the tissue regenerates plants having the genotype of ZS01301. The tissue culture is selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks and stalks, and cells and protoplasts thereof. The corn plant regenerated from ZS01301 having ZS01301&#39;s genotype. 
     The invention extends to hybrid seed produced by planting, in pollinating proximity, seeds of corn inbred lines ZS01301 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; and harvesting seeds produced on plants of the inbred. The hybrid seed produced by hybrid combination of plants of inbred corn seed designated ZS01301 and plants of another inbred line. Hybrid plants grown from this hybrid seed. 
     The invention further includes a method of hybrid F1 production. A first generation (F1) hybrid corn plant produced by the process of planting, in pollinating proximity, seeds of corn inbred lines ZS01301 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; harvesting seeds produced on plants of the inbred; and growing a harvested seed. 
     A tissue culture of the regenerable cells of hybrid plants produced with use of ZS01301 genetic material. A tissue culture of the regenerable cells of the corn plant produced by the method described above. 
     DEFINITIONS 
     In the description and examples which follow, a number of terms are used. In order to provide a clear and consistent understanding of the specifications and claims, including the scope to be given such terms, the following definitions are provided. 
     BL MOIST 
     The moisture percentage of the grain at black layer, i.e., when 50% of the plants per plot have reached physiological maturity. 
     COLD GERM 
     Cold Germ is a measurement of seed germination under cold soil conditions. Data is reported as percent of seed germinating. 
     ECB 
     European corn borer a maize eating insect. ECBI is the first brood generation of European corn borers. ECBII is the second generation of European corn borers. 
     EMERGE 
     The number of emerged plants per plot (planted at the same seedling rate) collected when plants have two fully developed leaves. 
     GI 
     This is a selection index which provides a single quantitative measure of the worth of a hybrid based on four traits. Yield is the primary trait contributing to index values. The GI value is calculated by combining stalk lodging, root lodging, yield and dropped ears according to the attached mathematical formula: 
     
         GI=100+0.5 (YLD)-0.9(% STALK LODGE)-0.9(% ROOT LODGE)-2.7(% DROPPED EAR) 
    
     GLS 
     Gray Leaf Spot (Cercospora Zeae) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     GW 
     Goss&#39; Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     HEATP10 
     The number of Growing Degree Units (GDU&#39;s) or heat units required for an inbred line or hybrid to have approximately 10 percent of the plants shedding pollen. This trait is measured from the time of planting. Growing Degree Units are calculated by the Barger Method where the GDU&#39;s for a 24 hour period are: ##EQU1## 
     The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDU&#39;s to reach various stages of plant development. 
     HEATBL 
     The number of GDU&#39;s after planting when approximately 50 percent of the inbred or hybrid plants in a plot have grain which has reached physiological maturity (black layer). 
     HEATPEEK 
     The number of GDU&#39;s after planting of an inbred when approximately 50 percent of the plants show visible tassel extension. 
     HEATP50 or HTP50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants shedding pollen. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATP90 
     The number of GDU&#39;s accumulated from planting when the last 100 percent of plants in an inbred or hybrid are still shedding enough viable pollen for pollination to occur. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS10 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 10 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS50 or HTS50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS90 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 90 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     MDMV A   
     Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     MDMV B   
     Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible and a &#34;9&#34; being very resistant.* 
     MOISTURE 
     The average percentage grain moisture of an inbred or hybrid at harvest time. 
     NLB 
     Northern Leaf Blight (Exserohilum turcicum) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     PCT TILLER 
     The total number of tillers per plot divided by the total number of plants per plot. 
     PLANT 
     This term includes plant cells, plant protoplasts, plant cell tissue cultures from which corn plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like. 
     PLANT HEIGHT 
     The distance in centimeters from ground level to the base of the tassel peduncle. 
     RM 
     Predicted relative maturity based on the moisture percentage of the grain at harvest. This rating is based on known set of checks and utilizes standard linear regression analyses and is referred to as the Minnesota Relative Maturity Rating System. 
     SHED 
     The volume of pollen shed by the male flower rated on a 1-9 scale where a &#34;1&#34; is a very light pollen shedder, a &#34;4.5&#34; is a moderate shedder, and a &#34;9&#34; is a very heavy shedder. If the Table(s) 3 have reduced the 1-9 shed scale to a 1-3 shed scale then any shed on Table 3 can be multiplied by 3 to reach the 1-9 shed scale. 
     SLB 
     Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     TWT 
     The measure of the weight of grain in pounds for a one bushel volume adjusted for percent grain moisture. 
     VIGOR 
     Visual rating of 1 to 9 made 2-3 weeks post-emergence where a &#34;1&#34; indicates very poor early plant development, and a &#34;9&#34; indicates superior plant development. 
     WARM GERM 
     A measurement of seed germination under ideal (warm, moist) conditions. Data is reported as percent of seeds germinating. 
     YIELD (YLD) 
     Actual yield of grain at harvest adjusted to 15.5% moisture. Measurements are reported in bushels per acre. 
     % DROPPED EARS (DE) 
     The number of plants per plot which dropped their primary ear divided by the total number of plants per plot. 
     % LRG FLAT 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen and a 14/64 inch slot screen, but does not pass through a screen with 20.5/64 inch round openings. 
     % LRG ROUND 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen, but does not pass through a 14/64 inch slot screen or a screen with 20.5/64 inch round openings. 
     % MED FLAT 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen and a 13/64 inch slotted screen, but does not pass through a screen with 17/64 inch round openings. 
     % MED ROUND 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen, but does not pass through a 13/64 inch slot screen or a screen with 17/64 inch round openings. 
     % SML FLAT 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen and a 12/64 inch slotted screen, but does not pass through a screen with 15/64 inch round openings. 
     % SML ROUND 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen, but does not pass through a 12/64 inch slotted screen or a screen with 15/64 inch round openings. 
     % ROOT LODGE (RL) 
     Percentage of plants per plot leaning more that 30 degrees from vertical divided by total plants per plot. 
     % STALK LODGE (SL) 
     Percentage of plants per plot with the stalk broken below the primary ear node divided by the total plants per plot. 
     * Resistant--on a scale of 1-9 with 9 evidencing the trait most strongly: 1-2.9 ratings are susceptible, 3-5.9 ratings are intermediate, and 6-9 ratings are resistant. 
     DETAILED DESCRIPTION OF THE INVENTION 
     ZS01301 can be used as a male line due to its pollen shed. This ZS01301 line evidences good general combining ability and specific combining ability with stiff stalk females. 
     The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. Most of the data in the Variety Description information was collected at Slater, Iowa. 
     The inbred has been self-pollinated for a sufficient number of generations to give inbred uniformity. During plant selection in each generation, the uniformity of plant type was selected to ensure homozygosity and phenotypic stability. The line has been increased in isolated farmland environments with data on uniformity and agronomic traits being observed to assure uniformity and stability. No variant traits have been observed or are expected in ZS01301. 
     The best method of producing the invention, ZS01301 which is substantially homozygous, is by planting the seed of ZS01301 which is substantially homozygous and self-pollinating or sib pollinating the resultant plant in an isolated environment, and harvesting the resultant seed or the resultant pollen. 
     
                                           TABLE 1__________________________________________________________________________ZS01301VARIETY DESCRIPTION INFORMATION#1 Type: Dent#2 Region Best Adapted: Southern zones of the central and western cornbeltregions. GRM = 115__________________________________________________________________________INBRED MO:ZS01301#3  MATURITYDAYS    HEATUNITS66  1609    FROM PLANTING TO 50% OF PLANTS IN SILK64  1560    FROM PLANTING TO 50% OF PLANTS IN POLLEN12          FROM 10% TO 90% POLLEN SHED#4 PLANTDATA1           ANTHOCYANIN OF BRACE ROOTS: 1 = ABSENT 2 = FAINT 3 = MODERATE       4 = DARK#5 LEAFCOLOR/DATA3/DARK GREEN       LEAF COLOR **MUNSELL CODE-5GY 4/42           LEAF SHEATH PUBESCENCE (1 = NONE TO 9 = PEACH FUZZ)5           MARGINAL WAVES (1 = NONE TO 9 = MANY)4           LONGITUDINAL CREASES (1 = NONE TO 9 = MANY)#6 TASSELCOLOR/DATA7           POLLEN SHED (0 = STERILE TO 9 = HEAVY SHEDDER)7/YELLOW    ANTHER COLOR **MUNSELL CODE-5Y 8/121/LIGHT GREEN       GLUME COLOR **MUNSELL CODE-2.5GY 8/41           BAR GLUME: 1 = ABSENT 2 = PRESENT#7A EAR (UNHUSKED DATA)COLOR/DATA12/LIGHT RED       SILK COLOR (3 DAYS AFTER EMERGE) **MUNSELL CODE-2.5R 7/42/MEDIUM GREEN       FRESH HUSK (25 DAYS AFTER 50% SILK) **MUNSELL CODE-5GY 6/622/TAN      DRY HUSK COLOR (65 DAYS AFTER 50% SILK **MUNSELL CODE-2.5Y       7/82           POSITION OF EAR AT DRY HUSK: 1 = UPRIGHT 2 = HORIZONTAL 3 =       PENDENT2           HUSK TIGHTNESS (1 = VERY LOOSE TO 9 = VERY TIGHT)2           HUSK EXTENSION AT HARVEST: 1 = EXPOSED EAR 2 = 8CM 3 = 8-10CM       4 = &gt; 10CM#7B EAR (HUSKED DATA)DATA1           KERNEL ROWS: 1 = INDISTINCT 2 = DISTINCT1           ROW ALIGNMENT: 1 = STRAIT 2 = SLIGHT CURVE 3 = SPIRAL2           EAR TAPPER 1 = STRAIT 2 = AVERAGE 3 = EXTREME#8 KERNEL (DRY)COLOR/DATA1           ALEURONE COLOR PATTERN: 1 = HOMO 2 = SEG7/YELLOW    ALEURONE COLOR **MUNSELL CODE-2.5Y 8/108/YELLOW-ORANGE       HARD ENDOSPERM COLOR **MUNSELL CODE-7.5YR 6/83           ENDOSPERM TYPE7/YELLOW    CROWN COLOR **MUNSELL CODE-2.5Y 8/10#9 COBCOLOR19/WHITE    COB COLOR **MUNSELL CODE-2.5Y 8/2__________________________________________________________________________1995 PROJECT MO PVP TRAITSINBRED ZS01301#10                     N MEAN STD.                              T-STAT                                   PROB                                       95% CI__________________________________________________________________________EAR HEIGHT(CM)          15                     61.07                          7.28                              32.47                                   0.0000                                       (57.38, 64.75)LENGTH OF PRIMARY EAR LEAF(CM)                   15                     70.13                          2.61                              103.9                                   0.0000                                       (68.81, 71.46)WIDTH OF PRIMARY EAR LEAF(CM)                   15                     9.21 0.71                              50.18                                   0.0000                                       (8.85, 9.57)TOP EAR INTERNODE(CM)   15                     12.95                          1.89                              26.47                                   0.0000                                       (11.99, 13.91)DEGREE OF LEAF ANGLE    15                     44.27                          8.67                              19.77                                   0.0000                                       (39.88, 48.66)# OF EARS PER PLANT     15                     1.13 0.35                              12.47                                   0.0000                                       (0.96, 1.31)# OF LEAVES ABOVE TOP EAR                   15                     5.27 0.46                              44.56                                   0.0000                                       (5.04, 5.50)# OF PRIMARY LATERAL TASSEL BRANCHES                   15                     5.33 0.90                              22.96                                   0.0000                                       (4.88, 5.79)PLANT HEIGHT(CM)        15                     155.3                          7.57                              79.41                                   0.0000                                       (151.4, 159.1)TASSEL LENGTH(CM)       15                     48.47                          1.96                              95.81                                   0.0000                                       (47.48, 49.46)TASSEL BRANCH ANGLE     15                     45.20                          9.62                              18.19                                   0.0000                                       (40.33, 50.07)# OF TILLER PER PLANTS  15                     0.00 0.00         (0.00, 0.00)WEIGHT PER 1 GG KERNELS(GM)                   15                     30.75                          3.57                              33.35                                   0.0000                                       (28.94, 32.55)EAR LENGTH(CM)          15                     19.73                          1.62                              47.05                                   0.0000                                       (18.91, 20.56)EAR WEIGHT(GM)          15                     107.0                          12.45                              33.29                                   0.0000                                       (100.7, 113.3)# OF KERNEL ROWS        15                     10.27                          0.70                              56.50                                   0.0000                                       (9.91, 10.62)COB DIAMETER AT MID-POINT(MM)                   15                     21.20                          1.37                              59.79                                   0.0000                                       (20.51, 21.89)EAR DIAMETER AT MID-POINT(MM)                   15                     37.53                          1.81                              80.43                                   0.0000                                       (36.62, 38.45)KERNEL LENGTH(MM)       15                     9.93 0.80                              48.16                                   0.0000                                       (9.53, 10.34)KERNEL THICKNESS(MM)    15                     5.20 0.86                              23.37                                   0.0000                                       (4.76, 5.64)KERNEL WIDTH(MM)        15                     9.47 0.64                              57.29                                   0.0000                                       (9.14, 9.79)% ROUND KERNELS(SHAPE GRADE)                   15                     57.20                          10.00                              22.15                                   0.0000                                       (52.14, 62.26)SHANK LENGTH            15                     10.13                          2.59                              15.17                                   0.0000                                       (8.82, 11.44)__________________________________________________________________________#11 DISEASE RESISTANCE -         Northern leaf blight = 5         NLSr2* = 8         Gray leaf spot = 7         Eye = 4.5         GW = 6         MDMVB = 1*Northern Leaf Spot Race Two#12 Other comparable inbreds are ZS0551, PVP#8200062, and__________________________________________________________________________PVP#9000050. 
    
     The Munsell code is a reference book of color which is known and used in the industry and by persons with ordinary skill in the art of plant breeding. 
     The purity and homozygosity of inbred ZS01301 is constantly being tracked using isozyme genotypes as shown in Table 2. 
     Isozyme Genotypes for ZS01301 
     Isozyme data were generated for inbred corn line ZS01301 according to procedures known and published in the art. The data in Table 2 gives the electrophoresis data on ZS01301. 
     
                                           TABLE 2__________________________________________________________________________ELECTROPHORESIS RESULTS FOR ZS01301INBREDACP1    ACP4        ADH MDH1                MDH2                    PGD1                        PGD2                            PH1                               PGM IDH2__________________________________________________________________________ZS0130111  55  22  11  22  11  11  22 22  22__________________________________________________________________________ 
    
     Inbred and Hybrid Performance of ZS01301 
     The traits and characteristics of inbred corn line ZS01301 are listed to compare with other inbreds and/or in hybrid combination. ZS01301 data shows the characteristics and traits of importance, giving a snapshot of ZS01301. 
     Table 3A shows a comparison between ZS01301 and ZS0551. ZS01301 has significantly lower plant and ear height than ZS0551. Its pollen flowering is slightly later than ZS0551 as shown by the HEATP10 and HEATP90 values. ZS01301 produces significantly less large round and flat seed sizes and more medium round seed. ZS01301 exhibits lower ratings for warm and cold germination, seedling vigor and emergence than ZS0551. 
     
                                           TABLE 3A__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                 PCT  PLANT                           EAR         EAR   PCTYEAR  INBRED      VIGOR EMERGE                 TILLER                      HEIGHT                           HEIGHT                                SHED   QUALITY                                             BARREN__________________________________________________________________________OVERALL ZS01301      5.4   66.5      179.3                           84.8 6.0 ZS0551      6.2   80.0      185.7                           88.9 6.4 # EXPTS      11    11        11   11   9 DIFF 0.9   13.5      6.4  4.1  0.4 PROB 0.000*            0.008*    0.066***                           0.099***                                0.035**__________________________________________________________________________YEAR  INBRED      HEATP10            HEATP50                 HEATP90                      HEATS10                           HEATS50                                HEATS90__________________________________________________________________________OVERALL ZS01301      1515  1573 1722 1538 1577 1630 ZS0551      1496  1555 1682 1520 1568 1613 # EXPTS      9     9    9    9    9    9 DIFF 19    18   40   18   10   18 PROB 0.066***            0.306                 0.004*                      0.222                           0.537                                0.264__________________________________________________________________________                 BL   % ROOT                           % STALK                                % DROPPEDYEAR  INBRED      HEATPEEK            HEATBL                 MOIST                      LODGE                           LODGE                                EARS   MOISTURE                                             YIELD__________________________________________________________________________OVERALL ZS01301      1409  2632 32.0                  15.2  89.4 ZS0551      1435  2626 31.5                  14.5  92.1 # EXPTS      9     2    1                     11    11 DIFF .25   7    0.5                   0.7   2.7 PROB 0.180 0.184                      0.572__________________________________________________________________________      WARM  COLD % LRG                      % LRG                           % MED                                % MED  % SML % SMLYEAR  INBRED      GERM  GERM ROUND                      FLAT ROUND                                FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01301      82.6  50.5 33.1 47.0 11.6 6.9    0.5   0.1 ZS0551      87.7  67.6 42.3 51.8 3.5  1.2    0.2   0.0 # EXPTS      8     8    10   10   10   10     10    9 DIFF 5.2   17.1 9.2  4.8  8.1  5.7    0.3   0.1 PROB 0.038**            0.025**                 0.031**                      0.074***                           0.000*__________________________________________________________________________ 
    
     Table 3B compares ZS01301 with PVP #8200062. Compared to PVP #8200062, ZS01301 is significantly taller and has greater seedling vigor but less emergence. ZS01301 flowers earlier for both pollen and silking as well as tassel emergence. ZS01301 has a significantly earlier black layer formation although the grain moisture at harvest is significantly greater. ZS01301 produces a significantly larger percent of layer flat kernels and less large and medium round kernels than PVP #8200062. 
     
                                           TABLE 3B__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                   PCT  PLANT                             EAR         EAR   PCTYEAR  INBRED VIGOR EMERGE                   TILLER                        HETGHT                             HEIGHT                                  SHED   QUALITY                                               BARREN__________________________________________________________________________OVERALL ZS01301        5.4   66.5      179.3                             84.8 6.1 PVP#8200062        4.8   82.7      166.0                             86.5 6.1 # EXPTS        1     11        11   11   8 DIFF   0.5   16.3      13.3 1.7  0.0 PROB   0.052***              0.005*    0.003*                             0.597                                  1.000__________________________________________________________________________YEAR  INBRED HEATP10              HEATP50                   HEATP90                        HEATS10                             HEATS50                                  HEATS90__________________________________________________________________________OVERALL ZS01301        1515  1573 1722 1538 1577 1630 PVP#8200062        1574  1633 1742 1606 1662 1696 # EXPTS        9     9    9    9    9    9 DIFF   59    60   20   68   85   66 PROB   0.006*              0.004*                   0.088***                        0.007*                             0.003*                                  0.012**__________________________________________________________________________                   BL   % ROOT                             % STALK                                  % DROPPEDYEAR  INBRED HEATPEEK              HEATBL                   MOIST                        LODGE                             LODGE                                  EARS   MOISTURE                                               YIELD__________________________________________________________________________OVERALL ZS01301        1409  2632 32.0                  15.2  89.4 PVP#8200062        1480  2658 29.5                  13.1  57.3 # EXPTS        9     2    1                     11    11 DIFF   71    25   2.5                   2.1   32.1 PROB   0.003*              0.099***                   0.031**__________________________________________________________________________        WARM  COLD % LRG                        % LRG                             % MED                                  % MED  % SML % SMLYEAR  INBRED GERM  GERM ROUND                        FLAT ROUND                                  FLAT   ROUND FLAT__________________________________________________________________________OVERALL 2501301        83.8  52.1 33.1 47.0 11.6 6.9    0.5   0.1 PVP#8200062        87.2  51.3 47.6 28.2 18.6 4.4    0.5   0.1 # EXPTS        9     9    .10  10   10   10     10    9 DIFF   3.4   0.8  14.5 18.7 6.9  2.5    0.0   0.0 PROB   0.278 0.921                   0.002*                        0.002*                             0.019**__________________________________________________________________________ 
    
     Table 3C compares ZS01301 with PVP #9000050. Compared to LH210, ZS01301 has significantly lower plant and ear height but reduced seedling vigor and emergence. ZS01301 sheds pollen and silks significantly earlier than PVP #9000050. Yield of ZS01301 is improved over PVP #9000050, and ZS01301 produced a significantly larger percent of large flat kernels and less medium round kernels. 
     
                                           TABLE 3C__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                   PCT  PLANT                             EAR         EAR   PCTYEAR  INBRED VIGOR EMERGE                   TILLER                        HEIGHT                             HEIGHT                                  SHED   QUALITY                                               BARREN__________________________________________________________________________OVERALL ZS01301        5.3   63.3      180.1                             85.4 6.0 PVP#9000050        6.6   87.2      212.1                             106  5.3 # EXPTS        10    10        10   10   9 DIFF   1.4   23.9      32.0 20.9 0.7 PROB   0.000*              0.000*    0.000*                             0.000*                                  0.316__________________________________________________________________________YEAR  INBRED HEATP10              HEATP50                   HEATP90                        HEATS10                             HEATS50                                  HEATS90__________________________________________________________________________OVERALL ZS01301        1515  1573 1722 1538 1577 1630 PVP#9000050        1649  1715 1802 1685 1728 1776 # EXPTS        9     9    9    9    9    9 DIFF   134   142  80   147  150  145 PROB   0.000*              0.000*                   0.000*                        0.000*                             0.000*                                  0.000*__________________________________________________________________________                   BL   % ROOT                             % STALK                                  % DROPPEDYEAR  INBRED HEATPEEK              HEATBL                   MOIST                        LODGE                             LODGE                                  EARS   MOISTURE                                               YIELD__________________________________________________________________________OVERALL ZS01301        1409  2632 32.0                  14.2  94.5 PVP#9000050        1551  2701 33.5                  14.3  40.1 # EXPTS        9     2    1                     10    10 DIFF   142   69   1.5                   0.1   54.4 PROB   0.000*              0.228                      0.911__________________________________________________________________________        WARM  COLD % LRG                        % LRG                             % MED                                  % MED  % SML % SMLYEAR  INBRED GERM  GERM ROUND                        FLAT ROUND                                  FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01301        83.8  52.1 33.1 47.0 11.6 6.9    0.5   0.1 PVP#9000050        93.8  74.7 38.8 22.0 28.3 9.2    0.6   0.6 # EXPTS        9     9    10   10   10   10     10    9 DIFF   10.0  22.6 5.7  25.0 16.7 2.3    0.1   0.5 PROB   0.005*              0.008*                   0.351                        0.000*                             0.000*__________________________________________________________________________ 
    
     Table 4 shows the GCA (general combining ability) estimates of ZS01301 compared with the GCA estimates of the other inbreds. The estimates show the general combining ability is weighted by the number of experiment/location combinations in which the specific hybrid combination occurs. The interpretation of the data for all traits is that a positive comparison is a practical advantage. A negative comparison is a practical disadvantage. The general combining ability of an inbred is clearly evidenced by the results of the general combining ability estimates. This data compares the inbred parent in a number of hybrid combinations to a group of &#34;checks&#34;. The check data is from other companies&#39; hybrids, particularly the leader in the industry and ICI Seeds&#39; commercial products and pre-commercial hybrids which were grown in the same sets and locations. 
     Table 4A shows ZS01301 crossed to 754 different inbreds. In hybrid combination, ZS01301 shows a positive GI index of 0.8 and a substantial yield advantage of 4.8. Moisture shows a disadvantage of -2.0, indicative of the late maturity of the inbred ZS01301. 
     
                                           TABLE 4A__________________________________________________________________________GCA &amp; SCA ESTIMATESADVANTAGES OVER COMMERICALS &amp; N&#39;S                  ENT                                              ZS01301N93    N94     N95        N  FI V M                 GI  I VLD MST                              % SL                                  % RL                                      % DE                                          TWT POP                                                 RM__________________________________________________________________________XR =    574  6  170        754           -3.9              -0.4                 0.8 4.5                       4.8 -2.0                              -1.8                                  0.1 -0.0                                          -0.7                                              -22                                                 124XH =         79 -5.1              -0.5                 -0.1                     5.4                       3.7 -2.2                              -1.9                                  -0.1                                      -0.1                                          -1.0                                              -43                                                 124XT =         16 -0.7              -0.2                 3.1 6.1                       9.0 -1.7                              -1.3                                  -0.2                                      -0.0                                          -0.6                                              -27                                                 124__________________________________________________________________________ XR = GCA ESTIMATE: WEIGHTED BY EXPT XM = GCA ESTIMATED: WEIGHTED BY PARENT2 XT = SAME AS XH, BUT USING ONLY THOSE PARENT2 WITH TWO YEARS OF DATA 
    
     Table 4B compares ZS01301 in random hybrid combinations with ZS0551, PVP #8200062 and PVP in random combinations. ZS01301 shows a comparable GI index, a greater yield advantage and greater moisture disadvantage. Hybrids with ZS01301 as a parent show slightly more tendency to stalk lodging but slightly less tendency to root lodging than do hybrids with either ZS0551, PVP #8200062 or PVP #9000050. 
     
                                           TABLE 4B__________________________________________________________________________       N   FI YM GI I  YLD                          MST                             % SL                                 % RL                                     % DE                                         TWT POP RM__________________________________________________________________________ZS0551  XR =       6531           -0.1              0.0                 0.5                    1.2                       3.4                          -0.3                             -0.6                                 -0.7                                     -0.0                                         -0.1                                             -92 123PVP#8200062   XR =       13352           -2.3              -0.3                 -0.4                    -0.6                       -0.2                          -0.8                             -0.1                                 -0.2                                     0.0 0.3 -34 123PVP#9000050   XR =       4421           0.4              0.0                 1.0                    0.7                       1.3                          -0.3                             0.4 -0.0                                     0.0 -0.7                                             -64 125__________________________________________________________________________ 
    
     Table 5A shows in positive numbers the advantage of ZS01301 hybrid has compared to two commercially available ICI Seeds&#39; hybrids. Compared to 8344, the ZS01301 hybrid is significantly higher yielding but later in maturity. Other agronomic performance traits of the ZS01301 hybrid and 8344 are essentially comparable. Compared to 8326IT, the ZS01301 hybrid is later in maturity, but has essentially comparable yield and agronomic performance traits. 
     
                                           TABLE 5A__________________________________________________________________________HYBRID SUMMARYZS01301/INBREDCORN PAIRED COMPARISONS__________________________________________________________________________HYBRID   YEAR        TESTS            GI ADV FI ADV YLD                             ADV MOIST                                      ADV SL__________________________________________________________________________ZS01301 RE 94  25  188               7   134                      5   183.9                             12.0*                                 23.5 -1.1*                                          2.28344  RE 94  25  181    129    171.9  22.4     1.9 RE 91-95        25  188               7   134                      5   183.9                             12.0*                                 23.5 -1.1*                                          2.2 RE 91-95        25  181    129    171.9  22.4     1.9ZS01301 RE 94  22  186               2   130                      -3  180.8                             -1.9                                 24.2 -2.0*                                          1.9832361T RE 94  22  184    133    182.7  22.2     3.5 RE 95  76  163               -1  110                      -3  137.9                             -1.8                                 23.0 -0.9*                                          6.7 RE 95  76  164    113    139.7  22.1     4.9 RE 91-95        98  168               -1  114                      -4  147.6                             -1.8                                 23.2 -1.1*                                          5.6 RE 91-95        98  169    118    149.4  22.1     4.6__________________________________________________________________________HYBRID   YEAR        TESTS            ADV RL ADV DE ADV TWT ADV POP ADV__________________________________________________________________________ZS01301 RE 94  25  -0.3                2.1                   1.6 0.2                          -0.1                              50.2                                  -0.9                                      25896                                          7658344  RE 94  25      3.7    0.1    51.1    25130 RE 91-95        25  -0.3                2.1                   1.6 0.2                          -0.1                              50.2                                  -0.9                                      25896                                          765 RE 91-95        25      3.7    0.1    51.1    25130ZS01301 RE 94  22  1.6 2.4                   1.5 0.2                          -0.1                              50.5                                  -0.2                                      24912                                          -283261T RE 94  22      3.9    0.1    50.6    24914 RE 95  76  -1.8                0.2                   0.7 0.1                          0.1 51.8                                  0.9 23643                                          -52 RE 95  76      0.9    0.2    50.9    23694 RE 91-95        98  -1.0                0.7                   0.9 0.1                          0.1 51.5                                  0.7 23928                                          -40 RE 91-95        98      1.6    0.2    50.8    23968__________________________________________________________________________ 
    
     Table 6A shows the entomology strengths and weaknesses of the inbred. 
     
                       TABLE 6A______________________________________                       EAR     PLANT  ECB1      ECB2       DAMAGE  INTEGRITYINBRED RATING    RATING     RATING  RATING______________________________________ZS01301  4.5       2.2        1       6.3______________________________________ 
    
     The inbred ZS01301 can be best employed as the male plant in a hybrid production field. This inbred is a medium tall line with moderate ear height. ZS01301 has a white cob color and produces large flat kernels which enhances its value as a parent of food grade hybrids. 
    
    
     The foregoing is set forth by way of example and is not intended to limit the scope of the invention. 
     This invention also is directed to methods for producing a corn plant by crossing a first parent corn plant with a second parent corn plant wherein the first or second parent corn plant is an inbred corn plant from the line ZS01301. Further, both first and second parent corn plants can come from the inbred corn line ZS01301. A variety of breeding methods can be selected depending on the mode of reproduction, the trait, the condition of the germplasm. Thus, any such methods using the inbred corn line ZS01301 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, haploid and anther culturing and the like. 
     Various culturing techniques known to those skilled in the art, such as haploid, transformation, and a host of other conventional and unconventional methods are within the scope of the invention. All plants and plant cells produced using inbred corn line ZS01301 are within the scope of this invention. The invention encompasses the inbred corn line used in crosses with other, different, corn inbreds to produce (F1) corn hybrid seeds and plants with the characteristics that make good hybrids. This invention includes cells which upon growth and differentiation produce corn plants having the physiological and morphological characteristics of the inbred line ZS01301. 
     Duncan, from at least 1985-1988 produced literature on plant regeneration from callus. Both inbred and hybrid callus have resulted in regenerated plants at excellent efficiency rates. Somatic embryogenesis has been performed on various maize tissue such as glume which before the 1980&#39;s was considered unusable for this purpose. The prior art clearly teaches the regeneration of plants from various maize tissues. 
     European Patent Application, publication 160,390, describes tissue culture of corn which can be used by those skilled in the art. Corn tissue culture procedures are also described in the literature as early as 1982. 
     A deposit of at least 2500 seeds of the inbred seed of this invention is maintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Access to this deposit will be available during the pendency of this application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. The Applicant made a deposit on Jun. 11, 1997, of at least 2500 seeds of Inbred Corn Line ZS01301 with the American Type Culture Collection (ATCC), located at 12301 Parklawn Drive, Rockville, Md. 20852. The ATCC accession number is 209110. Additionally the Applicant has satisfied all of the requirements of C.F.R. §1.801-1.809, including providing an indication of the viability of the sample. The ATCC deposit will be maintained in that depository, which is a public depository, for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. 
     Inbreds designated MBS are available from Mike Brayton Seed in Iowa. Inbreds designated SGI are available from Seed Genetic Inc. in New Jersey. Information on some ZS designations may be available from the PVP office. 
     Accordingly, the present invention has been described with some degree of particularity directed to the preferred embodiment of the present invention. It should be appreciated, though, that the present invention is defined by the following claims construed in light of the prior art so that modifications or changes may be made to the preferred embodiment of the present invention without departing from the inventive concepts contained herein.