Abstract:
A composition for the prevention or inhibition of free radical-induced effect on the skin and/or hair comprises at least three antioxidant agents selected from the group consisting of ginkgo extract, emblica extract, dimethylmethoxy chromanol, pine bark extract, and rosemary extract.

Description:
FIELD OF THE INVENTION 
       [0001]    This invention relates to compositions providing enhanced protection for the skin and hair against the adverse effects of free radicals, eg effects mediated by UV-radiation, or other sources of oxidative stress. In particular aspects, the invention provides topical compositions containing combinations of antioxidant agents effective in protecting the skin and/or hair against damage due to free radicals, and compositions intended for oral administration that provide systemic protection against such damage. 
       BACKGROUND OF THE INVENTION 
       [0002]    As we age, our skin undergoes changes such as becoming thinner, more easily damaged and less elastic. In addition, lifetime exposure to UV-A and UV-B radiation, together with other environmental factors that induce the formation of free radicals, such as pollution from traffic fumes, ozone, cigarette smoke etc, causes changes to the skin. These changes, including lines and wrinkling, actinic lentigines, dyspigmentation, rough skin, actinic telangiectasia and further loss of skin elastic function are due to direct UV-mediated damage to cells and indirectly mediated damage caused by the generation of free radicals in cells and tissues. This is generally termed photoageing and can account for up to 90% of the skin changes we associate with ageing. 
         [0003]    The deleterious effects of UV radiation are generally believed to be due to the creation of free radicals. These highly reactive species may react with and damage DNA molecules in the skin (or elsewhere). Similar effects can also be attributed to radiation in the visible part of the spectrum. 
         [0004]    Our hair also is damaged by exposure to UV-A and UV-B radiation, and other environmental factors that induce the formation of free radicals, such as those mentioned above. Furthermore, exposure to heat during drying and/or styling, and chemical treatment of the hair (eg perming, straightening, dyeing and/or bleaching) can also generate free radicals. Free radicals are implicated in the process of damage to hair, which may be observed as a reduction in shine, feel and/or fading of hair colour. 
         [0005]    It is known to use antioxidant compounds as free radical quenchers, thereby mitigating the effects of free radical formation. 
         [0006]    There have now been devised compositions and methods involving combinations of antioxidant agents which have been found to be particularly effective in protecting the skin and hair against free radical-induced damage. 
       BRIEF SUMMARY OF THE INVENTION 
       [0007]    The invention utilises combinations of antioxidant agents including at least three antioxidants selected from the following list (list A): 
         [0000]    ginkgo extract
 
emblica extract
 
dimethylmethoxy chromanol
 
pine bark extract
 
rosemary extract
 
         [0008]    In a first aspect, the invention provides a composition for the prevention or inhibition of free radical-induced effect on the skin and/or hair, which composition comprises at least three antioxidant agents selected from List A. 
         [0009]    In another aspect, the invention provides a method for the prevention or inhibition of free radical-induced effects on the skin and/or hair, which method comprises the administration to the hair and/or skin of a composition comprising at least three antioxidant agents selected from List A. 
         [0010]    The invention further comprises the use of at least three antioxidant agents selected from List A in the manufacture of a composition for the prevention or inhibition of free radical-induced effects on the skin and/or hair. 
         [0011]    The methods and compositions of the present invention are advantageous primarily in that they may protect the skin and/or hair more effectively from the effects of UV-induced free radical formation than known compositions. Therefore, the compositions and methods of the invention may be used to provide improved protection against damage to skin and/or hair caused by exposure to factors such as sunlight, environmental and/or atmospheric pollution. The improved protection may be due to achievement of a greater degree of protection, a greater duration of protection and/or a more rapid onset of protection. The method of the invention may have therapeutic benefits, but its primary effect may be cosmetic in that it improves or prevents degradation of the appearance of the skin and/or hair, eg due to the effects of exposure to external factors of the type that have been mentioned above, or in respect of the appearance of the skin due to ageing. 
         [0012]    Some or all of the antioxidant agents used in the present invention may already be known to be effective as free radical quenchers and to prevent oxidative damage to the skin and/or hair. However, the present invention discloses that combinations of these agents may have a greater efficacy than that expected, ie the efficacy of the combination is greater that the sum of the individual agents. The combinations of antioxidant agents described by the present invention are therefore “synergistic”. This has been demonstrated by in vitro testing. 
         [0013]    Antioxidant agents are often either highly coloured or else they are variable in colour or change colour over time. If highly coloured antioxidant agents are used in amounts necessary to be totally effective, it is likely that the agents would give the composition a cosmetically unacceptable appearance. It is similarly undesirable for cosmetic products to vary in colour or to change colour over time, which may falsely suggest to the consumer that the product is chemically deteriorating or is sub-standard in quality. Moreover, the use of antioxidant agents can be costly. For those reasons, inter alia, many conventional skincare and haircare compositions use less antioxidant agent(s) than necessary to provide total protection. Indeed, it may be the case that the base composition itself has a pro-oxidant effect, and therefore an increased concentration of antioxidant agent(s) is required to achieve the desired level of protection. With the present invention, because of the increased efficacy of the synergistic mixture of antioxidant agents, it is possible to include the antioxidant agents in sufficient amounts to provide an effective defense against the action of free radicals. Alternatively, if the same level of protection as a conventional formulation is required, then the increased efficacy of the synergistic mixture of antioxidant agents means that the composition will require lower quantities of the antioxidant agents than a conventional formulation. Hence, problems with highly coloured formulations may be reduced (cosmetic appearance), and the cost of the formulation is likely to be lower as well. 
         [0014]    The combinations of antioxidant agents according to the invention may be administered by a variety of routes. The antioxidants have been found to be particularly suitable for inclusion in compositions for topical administration to the skin and/or hair. However, the combinations of antioxidants are also suitable for systemic administration, in particular oral administration. 
         [0015]    Thus, according to a further aspect of the invention there is provided a composition for the prevention or inhibition of free radical-induced effects on the skin and/or hair, which composition is in a form suitable for oral administration and comprises at least three antioxidant agents selected from List A. 
         [0016]    In another aspect, the invention provides a method for the prevention or inhibition of free radical-induced effects on the skin and/or hair, which method comprises the oral administration of a composition comprising at least three antioxidant agents selected from List A. 
         [0017]    The invention further comprises the use of at least three antioxidant agents selected from List A, in the manufacture of a composition for the prevention or inhibition of free radical-induced effects on the skin and/or hair, the composition being in a form suitable for oral administration. 
         [0018]    It is generally preferred that the antioxidant agents selected from List A include at least one water-soluble antioxidant and at least one oil-soluble antioxidant. By water-soluble is meant that the antioxidant should be preferentially soluble in the water phase (ie aqueous phase) of a water-in-oil or an oil-in-water system. Similarly, by oil-soluble is meant that the antioxidant should preferentially be soluble in the oil phase of a water-in-oil or an oil-in-water system. By preferably soluble is meant that more than 50% by weight of the antioxidant dissolves in the respective water or oil phase. The water-soluble antioxidants may be such that more than 60%, more than 70%, more than 80%, or more than 90% by weight of the antioxidant dissolves in the water phase or the oil phase, respectively. 
         [0019]    In certain embodiments, the compositions of the invention comprise dimethylmethoxy chromanol in combination with at least two further antioxidants selected from List A. 
         [0020]    In combination, the amount of the at least three antioxidant agents selected from List A may range from 0.0001% to 10% by weight of the total composition, preferably from 0.001% to 1% by weight of the total composition and most preferably from 0.001% to 0.1% by weight of the total composition. 
         [0021]    Preferred compositions comprise ginkgo extract, emblica extract and a further antioxidant agent selected from List A, preferably dimethylmethoxy chromanol or rosemary extract, most preferably dimethylmethoxy chromanol. 
         [0022]    Preferred compositions comprise emblica extract, dimethylmethoxy chromanol and a further antioxidant agent selected from List A, most preferably ginkgo extract. 
         [0023]    Preferred compositions comprise emblica extract, rosemary extract and a further antioxidant agent selected from List A, preferably ginkgo extract or dimethylmethoxy chromanol. 
         [0024]    Preferred compositions comprise dimethylmethoxy chromanol, pine bark extract and a further antioxidant agent selected from List A, preferably emblica extract or rosemary extract, most preferably rosemary extract. 
         [0025]    Preferred compositions comprise dimethylmethoxy chromanol, rosemary extract and a further antioxidant agent selected from List A, preferably emblica extract or pine bark extract, most preferably pine bark extract. 
         [0026]    In one embodiment, compositions comprise dimethylmethoxy chromanol, pine bark extract and rosemary extract. Preferably the ratio by weight of dimethylmethoxy chromanol:pine bark extract:rosemary extract in the composition is D:P:R, where D is 15 to 25, P is 15 to 25 and R is 1. In certain embodiments, the ratio D:P:R is about 18:about 17:1. 
         [0027]    In another embodiment, compositions comprise emblica extract, dimethylmethoxy chromanol and rosemary extract. Preferably the ratio by weight of emblica extract:dimethylmethoxy chromanol:rosemary extract in the composition is E:D:R, where E is 10 to 20, D is 15 to 25 and R is 1. In certain embodiments, the ratio E:D:R is about 13:about 18:1. 
         [0028]    In another embodiment, compositions comprise emblica extract, dimethylmethoxy chromanol and pine bark extract. Preferably the ratio by weight of emblica extract:dimethylmethoxy chromanol:pine bark extract in the composition is E:D:P, where E is 10 to 20, D is 0.5 to 3 and P is 0.5 to 3. In certain embodiments, the ratio of E:D:P is about 13:about 1.8:about 1.7. 
         [0029]    In another embodiment, compositions comprise ginkgo extract, emblica extract and dimethylmethoxy chromanol. Preferably, the ratio by weight of ginkgo extract:emblica extract:dimethylmethoxy chromanol in the composition is G:E:D, where G is 1, E is 0.5 to 10, and D is 0.05 to 15. In certain embodiments G:E:D is about 18:about 13:about 1.8; about 1.8:about 13:about 18; about 20:about 15:about 20; about 5:about 15:about 20; or about 10:about 15:about 20. Most preferably the ratio is about 10:about 15:about 20. 
         [0030]    In a further embodiment, compositions comprise ginkgo extract, emblica extract and rosemary extract. Preferably the ratio by weight of ginkgo extract:emblica extract:rosemary extract in the composition is G:E:R, where G is 15 to 25, E is 10 to 20 and R is 1. In certain embodiments, the ratio is about 18:about 13:1. 
         [0031]    The antioxidants used in the invention are commercially available from numerous sources. 
         [0032]    The ginkgo extract that is preferred for use in the invention is an extract of the leaf of  Ginkgo biloba , for example Herbalia® Ginkgo obtained from Cognis Cognis Iberia S.L., Pg. Industrial San Vicente s/n, 08755 Castellbisbal, Barcelona, Spain. This product consists of 98%  Ginkgo biloba  leaf extract and 2% silica. It may be processed for use in the invention by making a solution in glycerin prior to its addition to the bulk composition at a temperature below 35° C. The amount of glycerin used to dissolve the ginkgo extract may be, for example, about 2% by weight of the total composition. 
         [0033]    The emblica extract preferred for use in the invention is a 100%  Phyllanthus emblica  fruit extract sold under the trade name Emblica™ by Merck Speciality Chemicals Merck KgaA, Dept. Pigments/Cosmetics, 64271 Darmstadt, Germany. It is understood that the major ingredients are emblicanin-A, emblicanin-B, pedunculagin, punigluconin, rutin and gallic acid; emblicanin-A, emblicanin-B and emblicanin-oligomers being the key active ingredients. About 90% of this extract is water-soluble and about 10% is oil-soluble. It may be processed for use in the invention by making a solution in water prior to addition to the bulk composition at a temperature below 35° C. The amount of water used to dissolve the emblica extract may be, for example, about 2% by weight of the total composition. 
         [0034]    Dimethylmethoxy chromanol is a synthetic analogue of gamma tocopherol (vitamin E). Other chemical names include 2H-1-benzopyran-6-ol and 3,4-dihydro-7-methoxy-2,2-dimethyl. It may be obtained from Lipotec S.A Poligon Industrial Camri Ral, C/Isaac Peral, 17 08850 Ga{acute over (v)}a, Barcelona, Spain, under the trade name Lipochroman-6. It is oil soluble, and may be processed for use in the invention by making a solution in ethanol prior to addition to the bulk composition at a temperature below 35° C. The amount of ethanol used to dissolve the dimethylmethoxy chromanol may be, for example, about 0.5% by weight of the total composition. 
         [0035]    Pine ( Pinus pinaster ) bark extract is a natural extract from the bark of the maritime pine tree, and may be obtained commercially under the trade name Oligopin from DRT, 30 Rue Gambetta, B. P. 206, 40105 DAX Cedex, France. Pine bark extract may also be obtained commercially under the trade name Pycnogenol®. It is partially oil soluble and partially water soluble, and may be processed for use in the invention by dissolving in 1:1 water:ethanol prior to addition to the bulk composition at a temperature below 35° C. 
         [0036]    Rosemary extract refers to extract of  Rosmarinus officinalis . It is commercially available, for example, as the Rosemary extract RA supplied by Vitiva, Nova vas 98, 2281 Markovci, Slovenia. It may be processed for use in the invention by dissolving in 1:1 water:ethanol prior to addition to the bulk composition at a temperature below 35° C. 
         [0037]    Preferred compositions may further comprise ascorbic acid, or a salt, ester, glucoside, glucosamine and/or other derivative thereof. The ascorbic acid component may comprise ascorbic acid itself, but more preferably comprises a derivative of ascorbic acid. Examples of such derivatives include salts, eg sodium and calcium ascorbate, or esters with inorganic and organic acids, eg ascorbyl phosphate and ascorbyl palmitate, or esters with glucose derivatives, eg ascorbyl glucoside. The ascorbic acid component of the composition may be the ascorbic acid-derived product sold as “Ester-C” by Inter-Cal Nutraceuticals of Prescott, Ariz., USA. That product is understood to include calcium ascorbate, together with one or more derivatives of aldonic acids, particularly threonic acid; that are metabolites of ascorbic acid. Most preferably, the ascorbic acid component of the composition is the ascorbyl glucoside sold as “AA2G” (Ascorbic Acid 2-Glucoside) by Hayashibara. 
         [0038]    The amount of ascorbic acid component in the composition may range from 0.0001% to 10% by weight of the total composition, preferably from 0.001% to 1% by weight of the total composition and most preferably from 0.01% to 0.1% by weight of the total composition. 
         [0039]    Compositions according to the invention may include additional active ingredients. 
         [0040]    Particularly preferred additional actives include one or more agents selected from the following list (List B): 
         [0000]    white lupin peptides
 
palmitoyl oligopeptide and palmitoyl tetrapeptides
 
retinyl palmitate.
 
         [0041]    Preferred compositions according to the invention comprise the following combination of agents from List A and List B: 
         [0042]    Dimethylmethoxy chromanol, pine bark extract, rosemary extract, white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides, and retinyl palmitate. 
         [0043]    Emblica extract, dimethylmethoxy chromanol, rosemary extract, white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides, and retinyl palmitate. 
         [0044]    Emblica extract, dimethylmethoxy chromanol, pine bark extract, white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides, and retinyl palmitate. 
         [0045]    Ginkgo extract, emblica extract, dimethylmethoxy chromanol, white lupin 20, peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides, and retinyl palmitate. 
         [0046]    Ginkgo extract, emblica extract, rosemary extract, white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides, and retinyl palmitate. 
         [0047]    Most preferably, the above combinations additionally comprise ascorbic acid, or a salt, ester, glucoside, glucosamine and/or other derivative thereof. 
         [0048]    The additional actives in List B are commercially available from numerous sources. 
         [0049]    White lupin peptides refers to a hydrolysed peptide extract of sweet white lupin ( lupinus albus ) commercially available, for example, from Silab, BP 213-19108 Brive Cedex, France. 
         [0050]    Palmitoyl oligopeptide and palmitoyl tetrapeptides may be obtained commercially as a single ingredient from Sederma, 29 Rue Du Chemin Vert BP 33, Le Perray En Yvelines, 78610 France, under the trade name Matrixyl™ 3000. 
         [0051]    Retinyl palmitate, or Vitamin A palmitate, may be obtained commercially, for example, from DSM Nutritional Products, Postfach, CH-4070 Basel, Switzerland. 
         [0052]    Additional active ingredients may include one or more further antioxidant ingredients. Preferred examples include: 
         [0000]    ginseng extract ( Panax ginseng );
 
mulberry concentrate ( Morus alba );
 
rose hip extract ( Rosa canine );
 
Japanese pagoda tree extract ( Sophora japonica );
 
lutein;
 
Edelweiss extract ( Leontopodium alpinum ) available commercially for example under the trade name Alpaflor Edelweiss GC (900-01) from Alpaflor;
 
rice bran oil ( Oryza sativa );
 
cranberry seed extract/oil ( Vaccinium macrocarpon ) commercially available for example under the trade name Cranberol™ from Chesham Chemicals Ltd;
 
alpha lipoic acid;
 
colourless carotenoids commercially available for example under the trade name IBR-CIC from Israeli Biotechnology Research;
 
Biodynes® O 3  (water and  saccaromyces  ferment filtrate lysate) commercially available from Arch Personal Care Products;
 
Einkorn extract ( Triticum monococcum ) available commercially for example under the trade name Phytoquintescine® from Vincience;
 
rooibus tea extract ( Aspalathus linearis ) available commercially for example as under the trade name Rooibus Herbasol from Cosmetochem Int Ltd;
 
Sweet Gale oil ( Myrica gale );
 
Castaline®, in particular Castaline® LS9763 commercially available from Cognis;
 
astaxanthin commercially available for example as Aastapure® Natural Astaxanthin from Alga Technologies.
 
         [0053]    In certain embodiments, the compositions according to the invention comprise the antioxidant agents selected from List A, optionally in combination with ascorbic acid or a derivative thereof, as described above, but are free or substantially free of other antioxidant agents. By “substantially free” in this context is meant that the composition contains less than 5%, or less than 2%, or less than 1%, or less than 0.1% w/w of other antioxidant agents. 
         [0054]    Compositions prepared in accordance with the invention may be formulated in any one of numerous different forms. Suitable types of composition, and methods by which they may be prepared, will generally be evident to those skilled in the art. 
       Topical Administration to the Skin 
       [0055]    The present invention can be delivered to the skin via any of the conventional formulations known to those skilled in the art. The formulations may be “skincare” or “personal care” compositions, for example compositions marketed as moisturisers, cleansers, toners, masks or scrubs etc, for the face or body. 
         [0056]    Equally, the compositions may be “cosmetic” compositions, which provide skincare benefits in addition to their principal cosmetic effect. Cosmetic formulations may be marketed, for example, as foundations, powders, lipsticks, eyeliners, eyeshadows, blushers, concealers, mascaras etc. 
         [0057]    Typical formulation types are creams, lotions, gels, serums and powders. 
         [0058]    One preferred group of compositions for application to the skin are formulated as emulsions. The emulsions may be o/w, w/o, o/w/o or w/o/w emulsions, which may be described inter alia as creams or lotions. Preferred emulsion compositions are o/w emulsions. 
         [0059]    One preferred group of compositions for application to the skin are formulated as gels using a suitable thickener or gelling agent such as acrylates/C10-30 alkyl acrylate crosspolymer. Gel compositions may contain alcohol. 
         [0060]    The composition will generally comprise other ingredients or excipients which constitute or form part of the dermatologically acceptable diluent or carrier and will be well known to those skilled in the art. These include, for example:
   a) Humectants, eg glycerin, propylene glycol, butylene glycol, hexylene glycol, dipropylene glycol, polyethylene glycol, sorbitol, urea, xylitol, lactitol, lactic acid and salts, fructose, glucose, mannose, xylose, honey, pyrrolidone, and carboxylic acid and salts.   b) Emollients, eg PPG-15 stearyl ether, ethylhexyl stearate, cetyl dimethicone, octyldodecanol, PPG-20 methyl glucose ether, isopropyl myristate isopropyl palmitate, isopropyl laurate isodecyl laurate, isodecyl neopentanoate, isohexadecane, pentaerythrityl tetraisostearate, caprylic/capric triglyceride, canola oil, sunflower oil ( Helianthus annuus ), olive oil ( Olea europea ), cottonseed oil ( Gossypium herbaceum ), jojoba oil ( Simmondsia chinensis ), shea butter ( Butyrospermum parkii ), cocoa butter ( Theobroma cacao ), cupuacu butter ( Theobroma grandiflorum ), avocado oil ( Persea gratissima ), liquid paraffin, dimethicone, phenyl trimethicone, cyclopentasiloxane, dimethiconol and petrolatum.
 
c) Surfactants—anionic surfactants, eg sodium lauryl sulphate, sodium laureth sulphate, ammonium laureth sulphate, disodium laureth sulfosuccinate and sodium C12-15 pareth sulphate; amphoteric/zwitterionic surfactants, eg cocamidopropyl betaine, sodium cocoamphoacetate and cocamidopropyl hydroxysultaine; nonionic surfactants, eg laureth-3, oleth-5, cocamide DEA, cocamide MEA, PEG-5 cocamide, polysorbate 20, PEG-40 hydrogenated castor oil; and cationic surfactants, eg cetrimonium chloride, behentrimonium chloride and benzalkonium chloride.
 
d) Emulsifiers, eg steareth-2, steareth-21, steareth-10, ceteareth-5, ceteareth-12, ceteareth-20, cetearyl glucoside, oleth-10, glyceryl stearate, polyglyceryl-3 oleate, polyglyceryl-3 methylglucose distearate, sodium cetearyl sulphate, sodium stearate, PEG-12 Oleate, PEG-2 stearate, PEG-12 stearate, PEG-80 sorbitan, sorbitan oleate, sorbitan palmitate and cetyl PEG/PPG-10/1 dimethicone.
 
e) Chelating agents or sequestering agents (sequestrants)—ingredients that have the ability to complex with and inactivate metallic ions in order to prevent their adverse effects on the stability or appearance of the composition. Examples of chelating agents are ethylenediamine tetraacetic acid and its salts, notably the dipotassium and especially the disodium or tetrasodium salt.
 
f) Sunscreening agents—inorganic sunscreening agents, eg microfine titanium dioxide, microfine zinc oxide, iron oxides, talcs and boron nitride; and/or organic sunscreening agents, eg p-aminobenzoic acids, esters and derivatives thereof, for example, 2-ethylhexyl p-dimethyl-aminobenzoate and the octyl ester of p-aminobenzoic acid; methoxycinnamate esters such as 2-ethylhexyl p-methoxycinnamate, 2-ethoxyethyl p-methoxycinnamate or α,β-di-(p-methoxycinnamoyl)-α′-(2-ethylhexanoyl)-glycerin; benzophenones such as oxybenzone; 2-phenylbenzimidazole-5-sulfonic acid and disodium phenyl dibenzimidazole tetrasulfonate and terphthalylidene dicamphor sulfonic acid; alkyl-β,β-diphenylacrylates for example alkyl α-cyano-β,β-diphenylacrylates such as octocrylene; triazines such as 2,4,6-trianilino-(p-carbo-2-ethylhexyl-1′-oxy)-1,3,5 triazine and bis-ethylhexyloxyphenol methoxyphenyl triazine; camphor derivatives such as methylbenzylidene camphor; organic pigment sunscreening agents such as methylene bis-benzotriazole tetramethyl butylphenol; silicone derivatives such as drometrizole trisiloxane, benzylidene malonate polysiloxane and dimethicodiethyl benzal malonate, salicylates such as octyl salicylate.
 
g) Preservatives—ingredients which prevent or retard microbial growth and thus protect the composition from spoilage. Examples of preservatives include DMDM hydantoin, propylparaben, methylparaben, phenoxyethanol, sodium benzoate, bronopol, sodium dehydroacetate, polyhexamethylenebiguanide hydrochloride, isothiazolone and diazolidinylurea.
 
h) Perfumes and colourings.
   
 
       Topical Administration to the Hair 
       [0063]    The present invention can be delivered to hair via any of the conventional formulations known to those skilled in the art, such as shampoos, conditioners (both emulsion and non-emulsion types), lotions (including developing lotions), sprays, gels, waxes, serums, mousses, tonics etc. Of these types of formulation, the range of ingredients can be broad. Such ingredients are surfactants, conditioning agents, waxes, thickeners, preservatives, and resins, sequestering agents, slip aids, vitamins, gelling agents, pearlising agents, pH adjusting agents and sunscreening agents and colours. 
         [0064]    The composition may include a surfactant such as cosmetically acceptable salts of alkyl ether sulphates (such as ammonium laureth sulphate or sodium laureth sulphate), alkyl and alkylamidoalkyl betaines (such as cocamidopropyl betaine), ethoxylated alcohols, polyethyleneglycol carboxylates, accepted salts of alkyl sulphates (such as ammonium lauryl sulphate or sodium lauryl sulphate), sulphosuccinates (such as disodium laureth sulphosuccinate), amphoacetates and amphodiacetates (such as disodium cocoamphodiacetates), alkylglucosides and alcohol sulphonates, incorporated in an amount of from about 1% to 99% by weight of the composition. 
         [0065]    The composition may also include a thickener or viscosity controlling agent such as an amine oxide, block polymers of ethylene oxide and propylene oxide (for examples, those available from BASF Wyandotte under the trade name “Pluronic”®), ethoxylated fatty alcohols, cellulosic derivatives (such as hydroxypropylmethyl cellulose), salt (NaCl), phthalic acid amide, polyvinylalcohols and fatty alcohols, suitably in an amount from about 0.5% to about 10% by weight of the composition. 
         [0066]    Sequestering agents may be added to the composition, such as ethylenediamine tetraacetic acid (EDTA) and salts thereof, suitably in an amount of from about 0.005% to about 0.5% by weight of the composition. 
         [0067]    Also included in the composition may be oils and waxes such as cocoa butter, suitably in an amount of from about 0.01% to about 1.0% by weight of the composition. 
         [0068]    The composition may also include gelling agents such as poly(methyl vinyl ether-co-maleic anhydride) (PVM/MA) or a decadiene crosspolymer (available under the trade name Stabileze® 06), suitably in an amount from about 0.1% to 2.0% by weight of the composition. 
         [0069]    Pearlising agents may be included eg stearic monoethanolamine, suitably in an amount from about 0.01% to about 10% by weight of the composition. The pH of the composition is generally required to be in the range of 8 to 12, preferably in the range of 9-10.5, for the desired performance as a permanent hair colour. To achieve this, the composition may need to be buffered using means well known in the art, such as a system comprising succinic acid, citric acid, lactic acid and acceptable salts thereof, phosphoric acid, mono- or disodium phosphate and sodium carbonate. The pH may be adjusted with an agent such as sodium hydroxide, aminomethyl propanol, triethanolamine and caustic potash, suitably in an amount from about 0.01% to about 10% by weight of the composition. 
         [0070]    If the composition is in the form of an emulsion, the emulsifiers used may be any emulsifiers known in the art for use in water-in-oil or oil-in-water emulsions, examples of which follow:
   a) sesquioleates such as sorbitan sesquioleate, available commercially for example under the trade name Arlacel 83 (ICI), or polyglyceryl-2-sesquioleate;   b) ethoxylated esters of derivatives of natural oils such as the polyethoxylated ester of hydrogenated castor oil available commercially for example under the trade name Arlacel 989 (ICI);   c) silicone emulsifiers such as silicone polyols available commercially for example under the trade name Dow Corning 5225C (Dow Corning) and Abil WE09 (Goldschmidt);   d) anionic emulsifiers such as fatty acid soaps eg potassium stearate and fatty acid sulphates eg sodium cetostearyl sulphate available commercially under the trade name Dehydag (Henkel);   e) ethoxylated fatty alcohols, for example the emulsifiers available commercially under the trade name Brij (ICI);   f) sorbitan esters, for example the emulsifiers available commercially under the trade name Span (ICI);   g) ethoxylated sorbitan esters, for example the emulsifiers available commercially under the trade name Tween (ICI);   h) ethoxylated fatty acid esters such as ethoxylated stearates, for example the emulsifiers available commercially under the trade name Myrj (ICI);   i) ethoxylated mono-, di-, and tri-glycerides, for example the emulsifiers available commercially under the trade name Labrafil (Alfa Chem.);   j) non-ionic self-emulsifying waxes, for example the wax available commercially under the trade name Polawax (Croda);   k) ethoxylated fatty acids, for example the emulsifiers available commercially under the trade name Tefose (Alfa Chem.); or   l) mixtures thereof.   
 
         [0083]    The amount of emulsifier present in the water-in-oil compositions of the present invention is preferably in the range 0.1 to 10%. 
       Oral Administration 
       [0084]    For oral administration, the active ingredient may be incorporated in a variety of dosage forms. Preferably, the active ingredient will be formulated and administered as a solid dosage form, most commonly as a tablet or the like. Other solid dosage forms include capsules, lozenges, powders and granules, and formulations such as a syrup (solution or suspension) may also be possible, as may other dosage forms. 
         [0085]    For formulation in the presently preferred form for oral administration, ie as a tablet, the active ingredient will generally be combined with various excipients in a manner which is known per se. In particular, the tablet will generally comprise one or more diluents or bulking agents. A lubricant may also be included to facilitate release of the formed tablets from the tableting dies of a tablet forming machine. 
         [0086]    Preferred materials for the diluent or bulking agents include calcium carbonate, polysaccharides and derivatives thereof, and saccharides. 
         [0087]    Polysaccharides which may be used include starch, eg maize starch, cellulose, eg powdered cellulose and microcrystalline cellulose, water-insoluble modified starches, eg sodium carboxymethyl starch, water-insoluble cellulose derivatives, eg croscarmellose sodium (cross-linked sodium carboxymethyl cellulose), cross-linked polyvinylpyrrolidone and alginic acid. 
         [0088]    Another preferred form of diluent is a saccharide. Suitable saccharides include, for example, sucrose, lactose, dextrose, sorbitol and xylitol. 
         [0089]    Particularly preferred diluents are microcrystalline cellulose, eg the products sold as Avicel PH-101 and Avicel PH-102 (Avicel is a Trade Mark) by the FMC Corporation of Philadelphia, Pa., USA, and lactose. 
         [0090]    The lubricant may be, for example, stearic acid, a metallic stearate, a polyethylene glycol of molecular weight of 4,000 or more, or purified talc. The preferred lubricant is a metallic stearate, particularly magnesium stearate. 
         [0091]    Other additional excipients may be included. Typical examples include flow aids, eg fused silica compounds, and disintegrants, including sodium starch glycolate, starch and crosscarmellose sodium. 
         [0092]    The tablet formulation may be prepared by methods that are familiar to those skilled in the art, eg processes involving wet or dry granulation or direct compression into a tablet without an intermediate, eg a wet or dry granulation, stage. 
       Test Methods 
       [0093]    The effectiveness of the antioxidant combinations of the present invention may be demonstrated by any suitable in vitro and in vivo test methods. Examples of currently available methods include the ABEL® antioxidant assay, and assays based on skin models, such as the EpiStem Human Skin Equivalent Model, and the Schrader assay. 
       ABEL® Antioxidant Assay 
       [0094]    The ABEL antioxidant assay is an in vitro assay for testing the antioxidant capacity of a material. It utilises a photoprotein, which emits light when challenged with various free radical species, eg peroxynitrite, hydroxyl radicals or halogenated oxidants. 
         [0095]    If a material is challenged with one or more of these free radical species, then any antioxidant in the sample will compete with the photoprotein. The result of this competition is a reduction in the amount of light emitted and sometimes, in addition, a delay in the time at which the maximum light emitted occurs. A light response curve can be produced for each sample tested, and EC 50  and ABEL-RAC values calculated. The EC 50  (effective concentration) is the concentration (normalised to g/L or mg/mL) of a material that reduces the light emitted by 50%. This reduction in light is a measure of the antioxidant capacity of the test material. The reciprocal of the EC 50  values multiplied by 100 is referred to as the ABEL-RAC score. 
         [0096]    The ABEL® antioxidant assay with peroxynitrite is the assay of choice to assess total antioxidant capacity because peroxynitrite attacks almost all compounds, including hydrophobic and hydrophilic antioxidants. Pro-oxidants are also identified with the peroxynitrite assay. Other ABEL® antioxidant assays use superoxide (produced as a single bolus in high concentration as well as enzymatically), hydroxyl radical and hyperchlorous acid (the halogenated oxidant assay). Pro-oxidants are not observed with the hydroxyl radical and halogenated oxidant assays. The hydroxyl radical assay is recommended when samples are water-soluble and the halogenated assay when the samples are soluble in organic solvent. This is because solvents such as ethanol, methanol, acetone and DMSO are good quenchers of hydroxyl radicals, and therefore the organic solvents contribute to the antioxidant effect shown by the sample. Organic solvents have little effect, however, on the halogenated oxidant assay. 
       EpiStem Human Skin Equivalent Model 
       [0097]    The EpiStem Human Skin Equivalent Model (DERMOQUANT®) is a highly differentiated in vitro skin equivalent model that is used to assess the efficacy and mode of action of novel agents. The model is generated from primary human keratinocytes on a collagen gel substrate containing human dermal fibroblasts. It is grown at the air-liquid interface which allows full epidermal stratification and epidermal interactions to occur. 
       Schrader Assay 
       [0098]    This is a commercial assay in which the test product is applied to the forearms of volunteers over a 2 week period and tape strips are taken. The antioxidant activity of the tape-strips is measured using a chemiluminescence method. 
     
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         [0099]      FIGS. 1 to 10  show the results of ABEL® peroxynitrite antioxidant assays for combinations of three antioxidant agents referred to as combinations 1 to 10 respectively. 
           [0100]      FIGS. 11 to 15  show the results of further ABEL® peroxynitrite antioxidant assays for combinations 1, 3, 4, 8 and 9 respectively. 
           [0101]      FIGS. 16 to 18  show the results of ABEL® peroxynitrite antioxidant assays for a cream containing combinations of three antioxidant agents, viz combinations 1, 8 and 8 mod respectively. 
           [0102]      FIG. 19  shows the results of the ABEL® peroxynitrite assay for dimethylmethoxy cromanol diluted with Industrial Methylated Spirits (IMS) and dimethylmethoxy cromanol diluted with ethanol. 
           [0103]      FIG. 20  shows the results of the ABEL® peroxynitrite assay for a cream containing a further combination of three antioxidant agents, referred to as combination 12. 
       
    
    
     DETAILED DESCRIPTION 
       [0104]    The invention will now be described in greater detail, by way of illustration only, with reference to the following Examples. Examples 1-28 provide example formulations, and Examples 29-34 describe the results of in vitro testing using the ABEL® peroxynitrite antioxidant assay. 
       Example 1 
     Skin Cream 
       [0105]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Paraffinum liquidum 
                 10 
               
               
                   
                 Cetearyl alcohol 
                 2 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Glyceryl stearate 
                 2 
               
               
                   
                 Cetyl alcohol 
                 2 
               
               
                   
                 PEG-100 stearate 
                 2 
               
               
                   
                 Dimethicone 
                 1.5 
               
               
                   
                 PEG-20 stearate 
                 0.5 
               
               
                   
                 Phenoxyethanol 
                 0.4 
               
               
                   
                 Carbomer 
                 0.2 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Propylparaben 
                 0.15 
               
               
                   
                 Potassium hydroxide 
                 0.06 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                   Ginkgo  extract 
                 0.005 
               
               
                   
                   Emblica  extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve tetrasodium EDTA. 
         2. Using homogenisation sprinkle in carbomer and continue to homogenise for 5 minutes or until hydrated. 
         3. Add methylparaben and heat up to 70-75° C. 
         4. In a separate vessel weigh out oil phase and heat to 70-75° C. (Paraffin liquidum, cetearyl alcohol, glyceryl stearate, cetyl alcohol, PEG-100 stearate, dimethicone, PEG-20 stearate, phenoxyethanol, retinyl palmitate and propylparaben.) 
         5. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. 
         6. Add potassium hydroxide and homogenise for 2 minutes. 
         7. Cool to room temperature. 
         8. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         9. Dissolve ginkgo extract in glycerin and stir into bulk. 
         10. Dissolve emblica extract in 2% water and stir into bulk. 
         11. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         12. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides 
         13. Make to weight with water and stir smooth. 
       
     
       Example 2 
     Skin Cream 
       [0119]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Paraffinum liquidum 
                 10 
               
               
                   
                 Cetearyl alcohol 
                 2 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Steareth-21 
                 2.94 
               
               
                   
                 Steareth-2 
                 2 
               
               
                   
                 Cetyl alcohol 
                 2 
               
               
                   
                 Dimethicone 
                 1.5 
               
               
                   
                 Phenoxyethanol 
                 0.4 
               
               
                   
                 Carbomer 
                 0.2 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Potassium hydroxide 
                 0.06 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Sodium ascorbyl phosphate 
                 0.05 
               
               
                   
                   Ginkgo  extract 
                 0.005 
               
               
                   
                   Emblica  extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve tetrasodium EDTA. 
         2. Using homogenisation sprinkle in carbomer and continue to homogenise for 5 minutes or until hydrated. 
         3. Add methylparaben and heat up to 70-75° C. 
         4. In a separate vessel weigh out oil phase and heat to 70-75° C. (Paraffin liquidum, cetearyl alcohol, steareth-21, steareth-2, cetyl alcohol, dimethicone, phenoxyethanol and retinyl palmitate) 
         5. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. 
         6. Add potassium hydroxide and homogenise for 2 minutes. 
         14. Cool to room temperature. 
         15. Dissolve sodium ascorbyl phosphate in 2% water and stir into bulk. 
         16. Dissolve ginkgo extract in glycerin and stir into bulk. 
         17. Dissolve emblica extract in 2% water and stir into bulk. 
         18. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         19. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides 
         20. Make to weight with water and stir smooth. 
       
     
       Example 3 
     Skin Cream with SPF 
       [0133]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 C12-15 alkyl benzoate 
                 5 
               
               
                   
                 Butyl methoxydibenzoylmethane 
                 3 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Cetearyl alcohol 
                 2 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Glyceryl stearate 
                 2 
               
               
                   
                 Cetyl alcohol 
                 2 
               
               
                   
                 PEG-100 stearate 
                 2 
               
               
                   
                 Dimethicone 
                 1.5 
               
               
                   
                 PEG-20 stearate 
                 0.5 
               
               
                   
                 Phenoxyethanol 
                 0.4 
               
               
                   
                 Carbomer 
                 0.2 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Propylparaben 
                 0.15 
               
               
                   
                 Potassium hydroxide 
                 0.06 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                   Ginkgo  extract 
                 0.005 
               
               
                   
                   Emblica  extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve tetrasodium EDTA. 
         2. Using homogenisation sprinkle in carbomer and continue to homogenise for 5 minutes or until hydrated. 
         3. Add methylparaben and heat up to 70-75° C. 
         4. In a separate vessel weigh out oil phase and heat to 70-75° C. (C12-15 alkyl benzoate, butyl methoxydibenzoylmethane, ethylhexyl methoxydibenzoylmethane, cetearyl alcohol, glyceryl stearate, cetyl alcohol, PEG-100 stearate, dimethicone, PEG-20 stearate, phenoxyethanol, retinyl palmitate and propylparaben) 
         5. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. 
         6. Add potassium hydroxide and homogenise for 2 minutes. 
         7. Cool to room temperature. 
         8. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         9. Dissolve ginkgo extract in glycerin and stir into bulk. 
         10. Dissolve emblica extract in 2% water and stir into bulk. 
         11. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         12. Stir into bulk White lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides 
         13. Make to weight with water and stir smooth. 
       
     
       Example 4 
     Gel 
       [0147]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Glycerin 
                 3 
               
               
                   
                 Acrylates/C10-30 alkyl acrylate 
                 1 
               
               
                   
                 crosspolymer 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Phenoxyethanol 
                 0.4 
               
               
                   
                 Potassium hydroxide 
                 0.29 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Methylparaben 
                 0.08 
               
               
                   
                 Ethylparaben 
                 0.05 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                   Ginkgo  extract 
                 0.005 
               
               
                   
                   Emblica  extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve tetrasodium EDTA. 
         2. Using homogenisation sprinkle in acrylates/C10-30 alkyl acrylate crosspolymer and continue to homogenise for 5 minutes or until hydrated. 
         3. Stir in potassium hydroxide to form gel. 
         4. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         5. Dissolve ginkgo extract in glycerin and stir into bulk. 
         6. Dissolve emblica extract in 2% water and stir into bulk. 
         7. Dissolve dimethylmethoxy chromanol and retinyl palmitate in alcohol denat. and stir into bulk. 
         8. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides 
         9. Make to weight with water and stir smooth. 
         10. Mix together phenoxyethanol, methylparaben and ethylparaben. Heat until a clear solution is formed. Stir into main vessel. 
         11. Make to weight with water and stir smooth. 
       
     
       Example 5 
     Gel 
       [0159]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Glycerin 
                 3 
               
               
                   
                 Acrylates/C10-30 alkyl acrylate 
                 1 
               
               
                   
                 crosspolymer 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Phenoxyethanol 
                 0.4 
               
               
                   
                 Potassium hydroxide 
                 0.29 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Methylparaben 
                 0.08 
               
               
                   
                 Ethylparaben 
                 0.05 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                   Ginkgo  extract 
                 0.018 
               
               
                   
                   Emblica  extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve tetrasodium EDTA. 
         2. Using homogenisation sprinkle in acrylates/C10-30 alkyl acrylate crosspolymer and continue to homogenise for 5 minutes or until hydrated. 
         3. Stir in potassium hydroxide to form gel. 
         4. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         5. Dissolve ginkgo extract in glycerin and stir into bulk. 
         6. Dissolve emblica extract in 2% water and stir into bulk. 
         7. Dissolve dimethylmethoxy chromanol and retinyl palmitate in Alcohol denat. and stir into bulk. 
         8. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides 
         9. Make to weight with water and stir smooth. 
         10. Mix together phenoxyethanol, methylparaben and ethylparaben. Heat until a clear solution is formed. Stir into main vessel. 
         11. Make to weight with water and stir smooth. 
       
     
       Example 6 
     Water-in-Oil Emulsion 
       [0171]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Paraffinum liquidum 
                 8 
               
               
                   
                 Ethylhexyl stearate 
                 7 
               
               
                   
                 Isopropyl myristate 
                 5 
               
               
                   
                 Glycerin 
                 3 
               
               
                   
                 Polyglyceryl-3 oleate 
                 1.7 
               
               
                   
                 Butylene glycol 
                 1.4 
               
               
                   
                 Cetyl PEG/PPG-10/1 dimethicone 
                 1.3 
               
               
                   
                 Sodium chloride 
                 0.75 
               
               
                   
                 Magnesium sulfate 
                 0.75 
               
               
                   
                 Phenoxyethanol 
                 0.7 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                   Ginkgo  extract 
                 0.005 
               
               
                   
                   Emblica  extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 White lupin peptides 
                 2 
               
               
                   
                 Palmitoyl oligopeptide and 
                 3 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add paraffin liquidum, ethylhexyl stearate, isopropyl myristate, polyglyceryl-3 oleate, cetyl PEG/PPG-10/1 dimethicone and retinyl palmitate. 
         2. Separately weigh out water, sodium chloride, magnesium sulfate, butylene glycol. 
         3. Separately mix together phenoxyethanol, methylparaben and ethylparaben. Heat until dissolved and add to the water phase. 
         4. Add the water phase to the oil phase slowly with constant stirring at high speed. Continue stirring for 5 minutes. 
         5. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         6. Dissolve ginkgo extract in glycerin and stir into bulk. 
         7. Dissolve emblica extract in 2% water and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         12. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides. 
         13. Homogenise the product for 5 minutes at 3500 rpm. 
       
     
       Example 7 
     Water-in-Oil Emulsion 
       [0182]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Paraffinum liquidum 
                 8 
               
               
                   
                 Ethylhexyl stearate 
                 7 
               
               
                   
                 Isopropyl myristate 
                 5 
               
               
                   
                 Glycerin 
                 3 
               
               
                   
                 Polyglyceryl-3 oleate 
                 1.7 
               
               
                   
                 Butylene glycol 
                 1.4 
               
               
                   
                 Cetyl PEG/PPG-10/1 dimethicone 
                 1.3 
               
               
                   
                 Sodium chloride 
                 0.75 
               
               
                   
                 Magnesium sulfate 
                 0.75 
               
               
                   
                 Phenoxyethanol 
                 0.7 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                   Ginkgo  extract 
                 0.018 
               
               
                   
                   Emblica  extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.001 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add paraffin liquidum, ethylhexyl stearate, isopropyl myristate, polyglyceryl-3 oleate, cetyl PEG/PPG-10/1 dimethicone and retinyl palmitate. 
         2. Separately weigh out water, sodium chloride, magnesium sulfate, butylene glycol. 
         3. Separately mix together phenoxyethanol, methylparaben and ethylparaben. Heat until dissolved and add to the water phase. 
         4. Add the water phase to the oil phase slowly with constant stirring at high speed. Continue stirring for 5 minutes. 
         5. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         6. Dissolve ginkgo extract in glycerin and stir into bulk. 
         7. Dissolve emblica extract in 2% water and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         9. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides. 
         10. Homogenise the product for 5 minutes at 3500 rpm. 
       
     
       Example 8 
     Hair Conditioner 
       [0193]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Cetyl alcohol 
                 4 
               
               
                   
                 Cetrimonium chloride 
                 3 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                   Ginkgo  extract 
                 0.018 
               
               
                   
                   Emblica  extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add water and cetrimonium chloride. Heat to 70-75° C. 
         2. In a separate vessel heat cetyl alcohol to 70-75° C. 
         3. Add the oil phase to the water phase and homogenise for 1 minute. 
         4. Cool to room temperature. 
         5. Stir in phenoxyethanol. 
         6. Dissolve ginkgo extract in glycerin and stir into bulk. 
         7. Dissolve emblica extract in 2% water and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         9. Make to weight with water and stir smooth. 
       
     
       Example 9 
     Hair Conditioner 
       [0203]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Cetyl alcohol 
                 4 
               
               
                   
                 Cetrimonium chloride 
                 3 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                   Ginkgo  extract 
                 0.01 
               
               
                   
                   Emblica  extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add water and cetrimonium chloride. Heat to 70-75° C. 
         2. In a separate vessel heat cetyl alcohol to 70-75° C. 
         3. Add the oil phase to the water phase and homogenise for 1 minute. 
         4. Cool to room temperature. 
         5. Stir in phenoxyethanol. 
         6. Dissolve ginkgo extract in glycerin and stir into bulk. 
         7. Dissolve emblica extract in 2% water and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         9. Make to weight with water and stir smooth. 
       
     
       Example 10 
     Body Wash 
       [0213]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Sodium laureth sulphate 
                 36 
               
               
                   
                 Cocamidopropyl betaine 
                 4 
               
               
                   
                 Cocamide DEA 
                 1.5 
               
               
                   
                 Sodium chloride 
                 1.5 
               
               
                   
                 Sodium benzoate 
                 0.3 
               
               
                   
                 Sodium methylparaben 
                 0.2 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Citric acid 
                 0.05 
               
               
                   
                 Sodium hydroxide 
                 0.05 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Ginkgo extract 
                 0.018 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Rosemary extract 
                 0.001 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve citric acid, sodium hydroxide, sodium chloride, sodium benzoate, sodium methylparaben and tetrasodium EDTA. 
         2. Stir in sodium laureth sulphate. 
         3. Stir in cocamide DEA. 
         4. Stir in cocamidopropyl betaine. 
         5. Dissolve ginkgo extract in glycerin and stir into bulk. 
         6. Dissolve emblica extract in 2% water and stir into bulk. 
         7. Dissolve rosemary extract in alcohol denat. and stir into bulk. 
         8. Make to weight with water and stir until uniform. 
       
     
       Example 11 
     Body Wash 
       [0222]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Sodium laureth sulphate 
                 36 
               
               
                   
                 Cocamidopropyl betaine 
                 4 
               
               
                   
                 Cocamide DEA 
                 1.5 
               
               
                   
                 Sodium chloride 
                 1.5 
               
               
                   
                 Sodium benzoate 
                 0.3 
               
               
                   
                 Sodium methylparaben 
                 0.2 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Citric acid 
                 0.05 
               
               
                   
                 Sodium hydroxide 
                 0.05 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Potassium hydroxide 
                 0.015. 
               
               
                   
                 Ginkgo extract 
                 0.018 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve citric acid, sodium hydroxide, sodium chloride, sodium benzoate, sodium methylparaben and tetrasodium EDTA. 
         2. Stir in sodium laureth sulphate. 
         3. Stir in cocamide DEA. 
         4. Stir in cocamidopropyl betaine. 
         5. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         6. Dissolve ginkgo extract in glycerin and stir into bulk. 
         7. Dissolve emblica extract in 2% water and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         9. Make to weight with water and stir until uniform. 
       
     
       Example 12 
     Styling Spray 
       [0232]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Alcohol denat. 
                 70 
               
               
                   
                 VP/VA copolymer 
                 5 
               
               
                   
                 Polyquaternium 11 
                 0.3 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 Rosemary extract 
                 0.0010 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add VPNA copolymer and polyquatemium 11. Stir until uniform (10 minutes) 
         2. In a separate vessel add alcohol denat., dimethylmethoxy chromanol, pine bark extract and rosemary extract. Stir until dissolved. Add this to the water phase and stir for 10 minutes until uniform. 
       
     
       Example 13 
     Styling Spray 
       [0235]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Alcohol denat. 
                 70 
               
               
                   
                 VP/VA copolymer 
                 5 
               
               
                   
                 Polyquaternium 11 
                 0.3 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add emblica extract, VPNA copolymer and polyquaternium 11. Stir until uniform (10 minutes). 
         2. In a separate vessel add alcohol denat., dimethylmethoxy chromanol and pine bark extract. Stir until dissolved. Add this to the water phase and stir for 10′ minutes until uniform. 
       
     
       Example 14 
     Lipstick 
       [0238]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Ricinus communis oil 
                 20 
               
               
                   
                 Phenyl trimethicone 
                 15 
               
               
                   
                 Polyethylene 
                 10 
               
               
                   
                 C10-30 Cholesterol/lanesterol 
                 10 
               
               
                   
                 esters 
               
               
                   
                 Octyldodecanol 
                 10 
               
               
                   
                 Hydrogenated polyisobutene 
                 8 
               
               
                   
                 Cera microcrystallina 
                 7 
               
               
                   
                 Mica 
                 5 
               
               
                   
                 Pigment 
                 5 
               
               
                   
                 Pearl 
                 10 
               
               
                   
                 Glycerin 
                 1 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Ginkgo extract 
                 0.018 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture: 
       [0000]    
       
         1. Disperse mica and pigment in  Ricinus ommunis  oil using an homogeniser until uniform. 
         2. Add phenyl trimethicone, polyethylene, C10-30 cholesterol/lanesterol esters, octyldodecanol, hydrogenated polyisobutene, Cera microcrystallina. 
         3. Heat to 80-85° C. 
         4. Stir in pearl. 
         5. Cool to 65° C. 
         6. Dissolve ginkgo extract in glycerin and stir into bulk. 
         7. Dissolve emblica extract in 1% water and stir into bulk 
         8. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         9. Mould to desired shape and cool. 
       
     
       Example 15 
     Mousse 
       [0248]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Cyclopentasiloxane 
                 72.9955 
               
               
                   
                 Mica 
                 11 
               
               
                   
                 Dimethicone crosspolymer 
                 7.5 
               
               
                   
                 Dimethicone copolyol 
                 2.5 
               
               
                   
                 Pigment 
                 4 
               
               
                   
                 Alcohol denat. 
                 1 
               
               
                   
                 Aqua 
                 1 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 Rosemary extract 
                 0.0010 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. Mix together cyclopentasiloxane and dimethicone copolyol. 
         2. Stir in mica and pigment until mixed and uniform (10 minutes). 
         3. Dissolve dimethylmethoxy chromanol, pine bark extract and rosemary extract in alcohol denat. Add aqua and stir into bulk. 
         4. Stir in dimethicone crosspolymer. 
       
     
       Example 16 
     Water-in-silicone Foundation Emulsion with SPF 
       [0253]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Cyclopentasiloxane 
                 20 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Talc 
                 4 
               
               
                   
                 Ethylhexyl stearate 
                 3 
               
               
                   
                 Mica 
                 3 
               
               
                   
                 Cyclohexasiloxane 
                 3 
               
               
                   
                 Dimethicone crosspolymer 
                 3 
               
               
                   
                 Dimethicone 
                 2.5 
               
               
                   
                 Silica 
                 2 
               
               
                   
                 Titanium dioxide 
                 2 
               
               
                   
                 Butylene glycol 
                 2 
               
               
                   
                 Disteardimonium hectorite 
                 1.5 
               
               
                   
                 Dimethicone copolyol 
                 1.2 
               
               
                   
                 Magnesium sulfate 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Cetyl PEG/PPG-10/1 dimethicone 
                 0.5 
               
               
                   
                 Hexyl Laurate 
                 0.5 
               
               
                   
                 Polyglyceryl-4 isostearte 
                 0.5 
               
               
                   
                 Propylene carbonate 
                 0.3 
               
               
                   
                 Stearic acid 
                 0.15 
               
               
                   
                 Triethoxycaprylylsilane 
                 0.13 
               
               
                   
                 Methyl paraben 
                 0.13 
               
               
                   
                 Butyl paraben 
                 0.03 
               
               
                   
                 Ethyl paraben 
                 0.03 
               
               
                   
                 Tetrasodium EDTA 
                 0.02 
               
               
                   
                 Propyl paraben 
                 0.02 
               
               
                   
                 Pigment 
                 10 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 1 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 Rosemary extract 
                 0.0010 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add cyclopentasiloxane, ethylhexyl methoxycinnamate, ethylhexyl stearate, cyclohexasiloxane, dimethicone, dimethicone copolyol, phenoxyethanol, cetyl PEG/PPG-10/1 dimethicone, hexyl laurate, polyglyceryl-4 isostearate, retinyl palmitate, methylparaben, butylparaben, ethylparaben, propylparaben. Heat to 40° C. 
         2. Disperse talc, mica, silica, titanium dioxide, stearic acid, triethoxycaprylsilane and pigment in the oil phase using an homogeniser until uniform (15 minutes). 
         3. Silverson in disteardimonium hectorite and propylene carbonate until uniform (5 minutes). 
         4. In a separate vessel weigh out water, butylene glycol, magnesium sulfate and tetrasodium EDTA. Stir until solids dissolve and heat to 40° C. 
         5. With both phases at 40° C. add the water phase slowly to the oil phase with stirring at high speed. 
         6. Cool below 35° C. 
         7. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol, pine bark extract, rosemary extract in alcohol denat. Stir in 1% water and stir into bulk. 
         9. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptide. 
         10. Homogenise for 5 minutes at 3500 rpm. 
         11. Stir in dimethicone crosspolymer and homogenise until smooth (2 minutes) 
       
     
       Example 17 
     Water-in-silicone Foundation Emulsion with SPF 
       [0265]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Cyclopentasiloxane 
                 20 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Talc 
                 4 
               
               
                   
                 Ethylhexyl stearate 
                 3 
               
               
                   
                 Mica 
                 3 
               
               
                   
                 Cyclohexasiloxane 
                 3 
               
               
                   
                 Dimethicone crosspolymer 
                 3 
               
               
                   
                 Dimethicone 
                 2.5 
               
               
                   
                 Silica 
                 2 
               
               
                   
                 Titanium dioxide 
                 2 
               
               
                   
                 Butylene glycol 
                 2 
               
               
                   
                 Disteardimonium hectorite 
                 1.5 
               
               
                   
                 Dimethicone copolyol 
                 1.2 
               
               
                   
                 Magnesium sulfate 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Cetyl PEG/PPG-10/1 dimethicone 
                 0.5 
               
               
                   
                 Hexyl Laurate 
                 0.5 
               
               
                   
                 Polyglyceryl-4 isostearte 
                 0.5 
               
               
                   
                 Propylene carbonate 
                 0.3 
               
               
                   
                 Stearic acid 
                 0.15 
               
               
                   
                 Triethoxycaprylylsilane 
                 0.13 
               
               
                   
                 Methyl paraben 
                 0.13 
               
               
                   
                 Butyl paraben 
                 0.03 
               
               
                   
                 Ethyl paraben 
                 0.03 
               
               
                   
                 Tetrasodium EDTA 
                 0.02 
               
               
                   
                 Propyl paraben 
                 0.02 
               
               
                   
                 Pigment 
                 10 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 1 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Rosemary extract 
                 0.001 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add cyclopentasiloxane, ethylhexyl methoxycinnamate, ethylhexyl stearate, cyclohexasiloxane, dimethicone, dimethicone copolyol, phenoxyethanol, cetyl PEG/PPG-10/1 dimethicone, hexyl laurate, polyglyceryl-4 isostearate, retinyl palmitate, methylparaben, butylparaben, ethylparaben, propylparaben. Heat to 40° C. 
         2. Disperse talc, mica, silica, titanium dioxide, stearic acid, triethoxycaprylsilane and pigment in the oil phase using an homogeniser until uniform (15 minutes). 
         3. Silverson in disteardimonium hectorite and propylene carbonate until uniform (5 minutes). 
         4. In a separate vessel weigh out water, butylene glycol, magnesium sulfate and tetrasodium EDTA. Stir until solids dissolve and heat to 40° C. 
         5. With both phases at 40° C. add the water phase slowly to the oil phase with stirring at high speed. 
         6. Cool below 35° C. 
         7. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol, and rosemary extract in alcohol denat. Stir in 1% water and stir into bulk. 
         9. Dissolve emblica extract in 2% water and stir into bulk. 
         10. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptide. 
         11. Homogenise for 5 minutes at 3500 rpm. 
         12. Stir in dimethicone crosspolymer and homogenise until smooth (2 minutes) 
       
     
       Example 18 
     Water-in-Silicone Foundation Emulsion with SPF 
       [0278]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Cyclopentasiloxane 
                 20 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Talc 
                 4 
               
               
                   
                 Ethylhexyl stearate 
                 3 
               
               
                   
                 Mica 
                 3 
               
               
                   
                 Cyclohexasiloxane 
                 3 
               
               
                   
                 Dimethicone crosspolymer 
                 3 
               
               
                   
                 Dimethicone 
                 2.5 
               
               
                   
                 Silica 
                 2 
               
               
                   
                 Titanium dioxide 
                 2 
               
               
                   
                 Butylene glycol 
                 2 
               
               
                   
                 Disteardimonium hectorite 
                 1.5 
               
               
                   
                 Dimethicone copolyol 
                 1.2 
               
               
                   
                 Magnesium sulfate 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Cetyl PEG/PPG-10/1 dimethicone 
                 0.5 
               
               
                   
                 Hexyl Laurate 
                 0.5 
               
               
                   
                 Polyglyceryl-4 isostearte 
                 0.5 
               
               
                   
                 Propylene carbonate 
                 0.3 
               
               
                   
                 Stearic acid 
                 0.15 
               
               
                   
                 Triethoxycaprylylsilane 
                 0.13 
               
               
                   
                 Methyl paraben 
                 0.13 
               
               
                   
                 Butyl paraben 
                 0.03 
               
               
                   
                 Ethyl paraben 
                 0.03 
               
               
                   
                 Tetrasodium EDTA 
                 0.02 
               
               
                   
                 Propyl paraben 
                 0.02 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Pigment 
                 10 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Ginkgo extract 
                 0.01 
               
               
                   
                 Emblica extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add cyclopentasiloxane, ethylhexyl methoxycinnamate, ethylhexyl stearate, cyclohexasiloxane, dimethicone, dimethicone copolyol, phenoxyethanol, cetyl PEG/PPG-10/1 dimethicone, hexyl laurate, polyglyceryl-4 isostearate, retinyl palmitate, methylparaben, butylparaben, ethylparaben, propylparaben. Heat to 40° C. 
         2. Disperse talc, mica, silica, titanium dioxide, stearic acid, triethoxycaprylsilane and pigment in the oil phase using an homogeniser until uniform (15 minutes). 
         3. Silverson in disteardimonium hectorite and propylene carbonate until uniform (5 minutes). 
         4. In a separate vessel weigh out water, butylene glycol, magnesium sulfate and tetrasodium EDTA. Stir until solids dissolve and heat to 40° C. 
         5. With both phases at 40° C. add the water phase slowly to the oil phase with stirring at high speed. 
         6. Cool below 35° C. 
         7. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         8. Dissolve ginkgo extract in glycerin and stir into bulk. 
         9. Dissolve dimethylmethoxy chromanol in alcohol denat. 
         10. Dissolve emblica extract in 2% water and stir into bulk. 
         11. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptide. 
         12. Homogenise for 5 minutes at 3500 rpm. 
         13. Stir in dimethicone crosspolymer and homogenise until smooth (2 minutes). 
       
     
       Example 19 
     Oil-in-Water Foundation Emulsion with SPF 
       [0292]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Butylene dlycol 
                 6 
               
               
                   
                 C12-15 alkyl benzoate 
                 5 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Cyclopentasiloxane 
                 3.6 
               
               
                   
                 Dimethicone 
                 3.5 
               
               
                   
                 Steareth-21 
                 3 
               
               
                   
                 Cetyl Alcohol 
                 2 
               
               
                   
                 Cyclohexasiloxane 
                 2 
               
               
                   
                 Titanium dioxide 
                 2 
               
               
                   
                 Steareth-2 
                 1.5 
               
               
                   
                 Mica 
                 1.2 
               
               
                   
                 Kaolin 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.8 
               
               
                   
                 Sodium polyacrylate 
                 0.3 
               
               
                   
                 Xanthan gum 
                 0.25 
               
               
                   
                 Methyl paraben 
                 0.25 
               
               
                   
                 Propylparaben 
                 0.15 
               
               
                   
                 Pigment 
                 14 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat 
                 1 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add water, butylene glycol, sodium polyacrylate. 
         2. Homogenise in titanium dioxide, mica, kaolin and pigment until uniform. 
         3. Homogenise in xanthan gum until uniform. Heat to 70-75° C. 
         4. In a separate vessel add C12-15 alkyl benzoate, ethylhexyl methoxycinnamate, cyclopentasiloxane, dimethicone, steareth-21, cetyl alcohol, cyclohexasiloxane, steareth-2, retinyl palmitate, phenoxyethanol, methylparaben and propylparaben. Heat to 70-75° C. 
         5. Add the oil phase to the water phase and homogenise for 5 minutes. 
         6. Cool below 35° C. 
         7. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         8. Dissolve emblica extract in 2% water and stir into bulk. 
         9. Dissolve dimethylmethoxy chromanol and pine bark extract in alcohol denat. Stir in 1% aqua and stir into bulk. 
         10. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides. 
         11. Make to weight and stir until smooth. 
       
     
       Example 20 
     Oil-in-Water Foundation Emulsion with SPF 
       [0304]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Butylene dlycol 
                 6 
               
               
                   
                 C12-15 alkyl benzoate 
                 5 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Cyclopentasiloxane 
                 3.6 
               
               
                   
                 Dimethicone 
                 3.5 
               
               
                   
                 Steareth-21 
                 3 
               
               
                   
                 Cetyl Alcohol 
                 2 
               
               
                   
                 Cyclohexasiloxane 
                 2 
               
               
                   
                 Titanium dioxide 
                 2 
               
               
                   
                 Steareth-2 
                 1.5 
               
               
                   
                 Mica 
                 1.2 
               
               
                   
                 Kaolin 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.8 
               
               
                   
                 Sodium polyacrylate 
                 0.3 
               
               
                   
                 Xanthan gum 
                 0.25 
               
               
                   
                 Methyl paraben 
                 0.25 
               
               
                   
                 Propylparaben 
                 0.15 
               
               
                   
                 Pigment 
                 14 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Ginkgo extract 
                 0.005 
               
               
                   
                 Emblica extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add 48% water, butylene glycol, sodium polyacrylate. 
         2. Homogenise in titanium dioxide, mica, kaolin and pigment until uniform. 
         3. Homogenise in xanthan gum until uniform. Heat to 70-75° C. 
         4. In a separate vessel add C12-15 alkyl benzoate, ethylhexyl methoxycinnamate, cyclopentasiloxane, dimethicone, steareth-21, cetyl alcohol, cyclohexasiloxane, steareth-2, retinyl palmitate, phenoxyethanol, methylparaben and propylparaben. Heat to 70-75° C. 
         5. Add the oil phase to the water phase and homogenise for 5 minutes. 
         6. Cool below 35° C. 
         7. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         8. Dissolve emblica extract in 2% water and stir into bulk. 
         9. Dissolve dimethylmethoxy chromanol in alcohol denat. stir into bulk. 
         10. Dissolve ginkgo extract in glycerin and stir into bulk. 
         11. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides. 
         12. Make to weight and stir until smooth. 
       
     
       Example 21 
     Eye Cream 
       [0317]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Caprylic/capric triglyceride 
                 5 
               
               
                   
                 Glycerin 
                 3 
               
               
                   
                 Petrolatum 
                 3 
               
               
                   
                 Helianthus annuus seed oil 
                 3 
               
               
                   
                 Cetearyl alcohol 
                 2.4 
               
               
                   
                 Paraffin 
                 2 
               
               
                   
                 Dimethicone 
                 1 
               
               
                   
                 Glyceryl stearate 
                 1 
               
               
                   
                 Cetyl alcohol 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 PEG-20 stearate 
                 0.6 
               
               
                   
                 Methylparaben 
                 0.25 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Carbomer 
                 0.2 
               
               
                   
                 Potassium hydroxide 
                 0.07 
               
               
                   
                 Tetrasodium EDTA 
                 0.02 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Ginkgo extract 
                 0.0018 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.018 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add water, add and dissolve tetrasodium EDTA. 
         2. With homogenisation sprinkle in carbomer. Continue to homogensie for 5 minutes. Add methylparaben and ethylparaben and heat to 70-75° C. 
         3. In a separate vessel add caprylic/capric triglyceride, petrolatum,  helianthus annuus  seed oil, cetearyl alcohol, paraffin, dimethicone, glyceryl stearate, cetyl alcohol, retinyl palmitate, phenoxyethanol and PEG-20 stearate. Heat to 70-75° C. 
         4. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. 
         5. Add potassium hydroxide and homogenise for 2 minutes. 
         6. Cool below 35° C. 
         7. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         8. Dissolve ginkgo extract in glycerin and stir into bulk. 
         9. Dissolve emblica in 2% water and stir into bulk. 
         10. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         11. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeprides. 
         12. Make to weight and stir smooth. 
       
     
       Example 22 
     Eve Cream 
       [0330]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Caprylic/capric triglyceride 
                 5 
               
               
                   
                 Glycerin 
                 3 
               
               
                   
                 Petrolatum 
                 3 
               
               
                   
                 Helianthus annuus seed oil 
                 3 
               
               
                   
                 Cetearyl alcohol 
                 2.4 
               
               
                   
                 Paraffin 
                 2 
               
               
                   
                 Dimethicone 
                 1 
               
               
                   
                 Glyceryl stearate 
                 1 
               
               
                   
                 Cetyl alcohol 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 PEG-20 stearate 
                 0.6 
               
               
                   
                 Methylparaben 
                 0.25 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Carbomer 
                 0.2 
               
               
                   
                 Potassium hydroxide 
                 0.07 
               
               
                   
                 Tetrasodium EDTA 
                 0.02 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Ginkgo extract 
                 0.005 
               
               
                   
                 Emblica extract 
                 0.015 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add water, add and dissolve tetrasodium EDTA. 
         2. With homogenisation sprinkle in carbomer. Continue to homogensie for 5 minutes. Add methylparaben and ethylparaben and heat to 70-75° C. 
         3. In a separate vessel add caprylic/capric triglyceride, petrolatum,  helianthus annuus  seed oil, cetearyl alcohol, paraffin, dimethicone, glyceryl stearate, cetyl alcohol, retinyl palmitate, phenoxyethanol and PEG-20 stearate. Heat to 70-75° C. 
         4. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. 
         5. Add potassium hydroxide and homogenise for 2 minutes. 
         6. Cool below 35° C. 
         7. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         8. Dissolve ginkgo extract in glycerin and stir into bulk. 
         9. Dissolve emblica in 2% water and stir into bulk. 
         10. Dissolve dimethylmethoxy chromanol in alcohol denat. and stir into bulk. 
         11. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeprides. 
         12. Make to weight and stir smooth. 
       
     
       Example 23 
     Body Lotion 
       [0343]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Paraffin liquidum 
                 20 
               
               
                   
                 Hydrogenated vegetable 
                 1 
               
               
                   
                 glycerides citrate 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Carbomer 
                 0.32 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.09 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 1 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 Rosemary extract 
                 0.0010 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add water, add and dissolve tetrasodium EDTA. 
         2. With homogenisation sprinkle in carbomer and homogenise for 2 minutes. Add methylparaben and ethylparaben and heat to 70-75° C. 
         3. In a separate vessel add paraffin liquidum, hydrogenated vegetable glycerides citrate, retinyl palmitate and phenoxyethanol. Heat to 70-75° C. 
         4. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. Add potassium hydroxide and homogenise for 2 minutes. 
         5. Cool below 35° C. 
         6. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         7. Dissolve dimethylmethoxy chromanol, pine bark extract and rosemary extract in alcohol denat. Stir in 1% water and stir into bulk. 
         8. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides. 
         9. Make to weight and stir smooth. 
       
     
       Example 24 
     Body Lotion 
       [0353]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Paraffin liquidum 
                 20 
               
               
                   
                 Hydrogenated vegetable 
                 1 
               
               
                   
                 glycerides citrate 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Carbomer 
                 0.32 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.09 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 1 
               
               
                   
                 Emblica extract 
                 0.013 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.0018 
               
               
                   
                 Rosemary extract 
                 0.001 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add water, add and dissolve tetrasodium EDTA. 
         2. With homogenisation sprinkle in carbomer and homogenise for 2 minutes. Add methylparaben and ethylparaben and heat to 70-75° C. 
         3. In a separate vessel add paraffin liquidum, hydrogenated vegetable glycerides citrate, retinyl palmitate and phenoxyethanol. Heat to 70-75° C. 
         4. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. Add potassium hydroxide and homogenise for 2 minutes. 
         5. Cool below 35° C. 
         6. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         7. Dissolve emblica in 2% water and stir into bulk. 
         8. Dissolve dimethylmethoxy chromanol and rosemary extract in alcohol denat. Stir in 1% water and stir into bulk. 
         9. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides. 
         10. Make to weight and stir smooth. 
       
     
       Example 25 
     Skin Cream 
       [0364]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Paraffinum liquidum 
                 10 
               
               
                   
                 Cetearyl alcohol 
                 2 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Glyceryl stearate 
                 2 
               
               
                   
                 Cetyl alcohol 
                 2 
               
               
                   
                 PEG-100 stearate 
                 2 
               
               
                   
                 Dimethicone 
                 1.5 
               
               
                   
                 PEG-20 stearate 
                 0.5 
               
               
                   
                 Phenoxyethanol 
                 0.4 
               
               
                   
                 Carbomer 
                 0.2 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Propylparaben 
                 0.15 
               
               
                   
                 Potassium hydroxide 
                 0.06 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Ginkgo extract 
                 0.005 
               
               
                   
                 Emblica extract 
                 0.015 
               
               
                   
                 Pine Bark Extract 
                 0.0017 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve tetrasodium EDTA. 
         2. Using homogenisation sprinkle in carbomer and continue to homogenise for 5 minutes or until hydrated. 
         3. Add methylparaben and heat up to 70-75° C. 
         4. In a separate vessel weigh out oil phase and heat to 70-75° C. (Paraffin liquidum, cetearyl alcohol, glyceryl stearate, cetyl alcohol, PEG-100 stearate, dimethicone, PEG-20 stearate, phenoxyethanol, retinyl palmitate and propylparaben.) 
         5. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. 
         6. Add potassium hydroxide and homogenise for 2 minutes. 
         21. Cool to room temperature. 
         22. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         23. Dissolve ginkgo extract in glycerin and stir into bulk. 
         24. Dissolve emblica extract in 2% water and stir into bulk. 
         25. Dissolve Pine bark extract in alcohol denat. and stir into bulk. 
         26. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides 
         27. Make to weight with water and stir smooth. 
       
     
       Example 26 
     Water-in-Silicone Foundation Emulsion with SPF 
       [0378]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
                 Aqua 
                 To 100 
               
               
                   
                 Cyclopentasiloxane 
                 20 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Talc 
                 4 
               
               
                   
                 Ethylhexyl stearate 
                 3 
               
               
                   
                 Mica 
                 3 
               
               
                   
                 Cyclohexasiloxane 
                 3 
               
               
                   
                 Dimethicone crosspolymer 
                 3 
               
               
                   
                 Dimethicone 
                 2.5 
               
               
                   
                 Silica 
                 2 
               
               
                   
                 Titanium dioxide 
                 2 
               
               
                   
                 Butylene glycol 
                 2 
               
               
                   
                 Disteardimonium hectorite 
                 1.5 
               
               
                   
                 Dimethicone copolyol 
                 1.2 
               
               
                   
                 Magnesium sulfate 
                 1 
               
               
                   
                 Phenoxyethanol 
                 0.6 
               
               
                   
                 Cetyl PEG/PPG-10/1 dimethicone 
                 0.5 
               
               
                   
                 Hexyl Laurate 
                 0.5 
               
               
                   
                 Polyglyceryl-4 isostearte 
                 0.5 
               
               
                   
                 Propylene carbonate 
                 0.3 
               
               
                   
                 Stearic acid 
                 0.15 
               
               
                   
                 Triethoxycaprylylsilane 
                 0.13 
               
               
                   
                 Methyl paraben 
                 0.13 
               
               
                   
                 Butyl paraben 
                 0.03 
               
               
                   
                 Ethyl paraben 
                 0.03 
               
               
                   
                 Tetrasodium EDTA 
                 0.02 
               
               
                   
                 Propyl paraben 
                 0.02 
               
               
                   
                 Pigment 
                 10 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 1 
               
               
                   
                 Emblica extract 
                 0.015 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 Rosemary extract 
                 0.0010 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add cyclopentasiloxane, ethylhexyl methoxycinnamate, ethylhexyl stearate, cyclohexasiloxane, dimethicone, dimethicone copolyol, phenoxyethanol, cetyl PEG/PPG-10/1 dimethicone, hexyl laurate, polyglyceryl-4 isostearate, retinyl palmitate, methylparaben, butylparaben, ethylparaben, propylparaben. Heat to 40° C. 
         2. Disperse talc, mica, silica, titanium dioxide, stearic acid, triethoxycaprylsilane and pigment in the oil phase using an homogeniser until uniform (15 minutes). 
         3. Silverson in disteardimonium hectorite and propylene carbonate until uniform (5 minutes). 
         4. In a separate vessel weigh out water, butylene glycol, magnesium sulfate and tetrasodium EDTA. Stir until solids dissolve and heat to 40° C. 
         5. With both phases at 40° C. add the water phase slowly to the oil phase with stirring at high speed. 
         12. Cool below 35° C. 
         13. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         14. Dissolve pine bark extract, rosemary extract in alcohol denat. Stir in 1% water and stir into bulk. 
         15. Dissolve emblica extract in 2% water and stir into bulk. 
         16. Stir in white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptide. 
         17. Homogenise for 5 minutes at 3500 rpm. 
         18. Stir in dimethicone crosspolymer and homogenise until smooth (2 minutes) 
       
     
       Example 27 
     Skin Cream with SPF 
       [0391]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 C12-15 alkyl benzoate 
                 5 
               
               
                   
                 Butyl methoxydibenzoylmethane 
                 3 
               
               
                   
                 Ethylhexyl methoxycinnamate 
                 5 
               
               
                   
                 Cetearyl alcohol 
                 2 
               
               
                   
                 Glycerin 
                 2 
               
               
                   
                 Glyceryl stearate 
                 2 
               
               
                   
                 Cetyl alcohol 
                 2 
               
               
                   
                 PEG-100 stearate 
                 2 
               
               
                   
                 Dimethicone 
                 1.5 
               
               
                   
                 PEG-20 stearate 
                 0.5 
               
               
                   
                 Phenoxyethanol 
                 0.4 
               
               
                   
                 Carbomer 
                 0.2 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Propylparaben 
                 0.15 
               
               
                   
                 Potassium hydroxide 
                 0.06 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 Tetrasodium EDTA 
                 0.05 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Ginkgo extract 
                 0.005 
               
               
                   
                 Pine bark extract 
                 0.0017 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 White lupin peptides 
                 1 
               
               
                   
                 Palmitoyl oligopeptide and 
                 1.5 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. To water add and dissolve tetrasodium EDTA. 
         2. Using homogenisation sprinkle in carbomer and continue to homogenise for 5 minutes or until hydrated. 
         3. Add methylparaben and heat up to 70-75° C. 
         4. In a separate vessel weigh out oil phase and heat to 70-75° C. (C12-15 alkyl benzoate, butyl methoxydibenzoylmethane, ethylhexyl methoxydibenzoylmethane, cetearyl alcohol, glyceryl stearate, cetyl alcohol, PEG-100 stearate, dimethicone, PEG-20 stearate, phenoxyethanol, retinyl palmitate and propylparaben) 
         5. With both phases at 70-75° C. add the oil phase to the water phase and homogenise for 2 minutes. 
         6. Add potassium hydroxide and homogenise for 2 minutes. 
         14. Cool to room temperature. 
         15. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         16. Dissolve gingko extract in glycerin and stir into bulk. 
         17. Dissolve dimethylmethoxy chromanol and pine bark extract in alcohol denat. Stir in 1% water and stir into bulk. 
         18. Stir into bulk White lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides 
         19. Make to weight with water and stir smooth. 
       
     
       Example 28 
     Water-in-Oil Emulsion 
       [0404]      
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Material 
                 % w/w 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Paraffinum liquidum 
                 8 
               
               
                   
                 Ethylhexyl stearate 
                 7 
               
               
                   
                 Isopropyl myristate 
                 5 
               
               
                   
                 Glycerin 
                 3 
               
               
                   
                 Polyglyceryl-3 oleate 
                 1.7 
               
               
                   
                 Butylene glycol 
                 1.4 
               
               
                   
                 Cetyl PEG/PPG-10/1 dimethicone 
                 1.3 
               
               
                   
                 Sodium chloride 
                 0.75 
               
               
                   
                 Magnesium sulfate 
                 0.75 
               
               
                   
                 Phenoxyethanol 
                 0.7 
               
               
                   
                 Methylparaben 
                 0.2 
               
               
                   
                 Ethylparaben 
                 0.1 
               
               
                   
                 Ascorbyl glucoside 
                 0.05 
               
               
                   
                 Potassium hydroxide 
                 0.015 
               
               
                   
                 Ginkgo extract 
                 0.005 
               
               
                   
                 Rosemary extract 
                 0.001 
               
               
                   
                 Dimethylmethoxy chromanol 
                 0.02 
               
               
                   
                 Alcohol denat. 
                 0.5 
               
               
                   
                 White lupin peptides 
                 2 
               
               
                   
                 Palmitoyl oligopeptide and 
                 3 
               
               
                   
                 Palmitoyl tetrapeptides 
               
               
                   
                 Retinyl palmitate 
                 0.07 
               
               
                   
                 Aqua 
                 To 100 
               
               
                   
                   
               
             
          
         
       
     
       Method of Manufacture 
       [0000]    
       
         1. In the main vessel add paraffin liquidum, ethylhexyl stearate, isopropyl myristate, polyglyceryl-3 oleate, cetyl PEG/PPG-10/1 dimethicone and retinyl palmitate. 
         2. Separately weigh out water, sodium chloride, magnesium sulfate, butylene glycol. 
         3. Separately mix together phenoxyethanol, methylparaben and ethylparaben. Heat until dissolved and add to the water phase. 
         9. Add the water phase to the oil phase slowly with constant stirring at high speed. Continue stirring for 5 minutes. 
         10. Dissolve ascorbyl glucoside in 2% water, neutralise with potassium hydroxide and stir into bulk. 
         11. Dissolve gingko extract in glycerin and stir into bulk. 
         12. Dissolve dimethylmethoxy chromanol and rosemary extract in alcohol denat. Add 1% water and stir into bulk. 
         13. Stir into bulk white lupin peptides, palmitoyl oligopeptide and palmitoyl tetrapeptides. 
         14. Homogenise the product for 5 minutes at 3500 rpm. 
       
     
       Example 29 
     Antioxidant Testing for Combinations of Three Antioxidant Agents Selected from List A 
     Method 
       [0000]    
       
         1. The antioxidant agents (referred to as raw materials (RM) hereafter) were first dissolved in an appropriate solvent as detailed in Table 1. 
         2. Combinations of three raw materials were prepared for testing by combining one part each of three raw material dilutions, at the concentrations specified in Table 2. 
         3. In Table 2 each combination is given a combination number and each combination number is further described as A, B or C, where the concentrations of the raw material dilutions used in B are ten times greater that A, and the concentrations used in C are ten times greater than B (one hundred times greater than A). 
         4. The concentrations of raw materials provided in Table 2 are the final concentrations of those materials in the ABEL® antioxidant assay. 
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Raw materials and solvents used 
               
             
          
           
               
                   
                   
                 RM 
                   
               
               
                   
                 RM name 
                 number 
                 Solvent 
               
               
                   
                   
               
             
          
           
               
                   
                 ginkgo extract 
                 2 
                 1:1 IMS:water 
               
               
                   
                 emblica extract 
                 9 
                 water 
               
               
                   
                 dimethylmethoxy chromanol 
                 10 
                 IMS 
               
               
                   
                 pine bark extract 
                 15 
                 1:1 IMS:water 
               
               
                   
                 rosemary extract 
                 19 
                 1:1 IMS:water 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Concentrations of raw materials used in each combination tested 
               
             
          
           
               
                   
                 All concentrations are mg/mL and are the final 
               
               
                 Combi- 
                 concentrations in the ABEL ® assay for peroxynitrite 
               
             
          
           
               
                 nation 
                 RM 
                 RM conc 
                 RM 
                 RM conc 
                 RM 
                 RM conc 
               
               
                 Number 
                 number 
                 (%) 
                 number 
                 (%) 
                 number 
                 (%) 
               
               
                   
               
             
          
           
               
                 1A 
                 10 
                 0.00018 
                 15 
                 0.00017 
                 19 
                 0.0001 
               
               
                 1B 
                 10 
                 0.0018 
                 15 
                 0.0017 
                 19 
                 0.001 
               
               
                 1C 
                 10 
                 0.018 
                 15 
                 0.017 
                 19 
                 0.01 
               
               
                 2A 
                 9 
                 0.0013 
                 15 
                 0.00017 
                 19 
                 0.0001 
               
               
                 2B 
                 9 
                 0.013 
                 15 
                 0.0017 
                 19 
                 0.001 
               
               
                 2C 
                 9 
                 0.13 
                 15 
                 0.017 
                 19 
                 0.01 
               
               
                 3A 
                 9 
                 0.0013 
                 10 
                 0.00018 
                 19 
                 0.0001 
               
               
                 3B 
                 9 
                 0.013 
                 10 
                 0.0018 
                 19 
                 0.001 
               
               
                 3C 
                 9 
                 0.13 
                 10 
                 0.018 
                 19 
                 0.01 
               
               
                 4A 
                 9 
                 0.0013 
                 10 
                 0.00018 
                 15 
                 0.00017 
               
               
                 4B 
                 9 
                 0.013 
                 10 
                 0.0018 
                 15 
                 0.0017 
               
               
                 4C 
                 9 
                 0.13 
                 10 
                 0.018 
                 15 
                 0.017 
               
               
                 5A 
                 2 
                 0.0018 
                 15 
                 0.00017 
                 19 
                 0.0001 
               
               
                 5B 
                 2 
                 0.018 
                 15 
                 0.0017 
                 19 
                 0.001 
               
               
                 5C 
                 2 
                 0.18 
                 15 
                 0.017 
                 19 
                 0.01 
               
               
                 6A 
                 2 
                 0.0018 
                 10 
                 0.00018 
                 15 
                 0.00017 
               
               
                 6B 
                 2 
                 0.018 
                 10 
                 0.0018 
                 15 
                 0.0017 
               
               
                 6C 
                 2 
                 0.18 
                 10 
                 0.018 
                 15 
                 0.017 
               
               
                 7A 
                 2 
                 0.0018 
                 9 
                 0.0013 
                 15 
                 0.00017 
               
               
                 7B 
                 2 
                 0.018 
                 9 
                 0.013 
                 15 
                 0.0017 
               
               
                 7C 
                 2 
                 0.18 
                 9 
                 0.13 
                 15 
                 0.017 
               
               
                 8A 
                 2 
                 0.0018 
                 9 
                 0.0013 
                 10 
                 0.00018 
               
               
                 8B 
                 2 
                 0.018 
                 9 
                 0.013 
                 10 
                 0.0018 
               
               
                 8C 
                 2 
                 0.18 
                 9 
                 0.13 
                 10 
                 0.018 
               
               
                 9A 
                 2 
                 0.0018 
                 9 
                 0.0013 
                 19 
                 0.0001 
               
               
                 9B 
                 2 
                 0.018 
                 9 
                 0.013 
                 19 
                 0.001 
               
               
                 9C 
                 2 
                 0.18 
                 9 
                 0.13 
                 19 
                 0.01 
               
               
                 10A  
                 2 
                 0.0018 
                 10 
                 0.00018 
                 19 
                 0.0001 
               
               
                 10B  
                 2 
                 0.018 
                 10 
                 0.0018 
                 19 
                 0.001 
               
               
                 10C  
                 2 
                 0.18 
                 10 
                 0.018 
                 19 
                 0.01 
               
               
                   
               
             
          
         
       
     
         [0418]      FIGS. 1-10  show the results of the ABEL® antioxidant assay for peroxynitrite, for each of combinations 1-10. 
       SUMMARY 
       [0419]    Raw material combinations 1, 3, 4, 8 and 9 gave the best synergies or displayed a delay in the time to peak, and were therefore the combinations that were analysed further. 
       Example 30 
     Further Antioxidant Testing on Selected Combinations of Three Antioxidant Agents from List A 
       [0420]    Raw material combinations 1, 3, 4, 8 and 9 were assayed at three additional concentrations which are dilutions of concentration A (see Table 2). The details are contained in Table 3. 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Concentrations of raw materials used in each combination tested 
               
             
          
           
               
                   
                 All concentrations are mg/mL and are the final 
               
               
                 Combi- 
                 concentrations used in the ABEL ® assay for peroxynitrite 
               
             
          
           
               
                 nation 
                 RM 
                 RM conc 
                 RM 
                 RM conc 
                 RM 
                 RM conc 
               
               
                 number 
                 number 
                 (%) 
                 number 
                 (%) 
                 number 
                 (%) 
               
               
                   
               
             
          
           
               
                 1 
                 10 
                 0.00018 
                 15 
                 0.000170 
                 19 
                 0.00010 
               
               
                 1 
                 10 
                 0.00009 
                 15 
                 0.000085 
                 19 
                 0.00005 
               
               
                 1 
                 10 
                 0.000036 
                 15 
                 0.000034 
                 19 
                 0.00002 
               
               
                 1 
                 10 
                 0.000018 
                 15 
                 0.000017 
                 19 
                 0.00001 
               
               
                 3 
                 9 
                 0.0013 
                 10 
                 0.000180 
                 19 
                 0.00010 
               
               
                 3 
                 9 
                 0.00065 
                 10 
                 0.000090 
                 19 
                 0.00005 
               
               
                 3 
                 9 
                 0.00026 
                 10 
                 0.000036 
                 19 
                 0.00002 
               
               
                 3 
                 9 
                 0.00013 
                 10 
                 0.000018 
                 19 
                 0.00001 
               
               
                 4 
                 9 
                 0.0013 
                 10 
                 0.000180 
                 15 
                 0.00017 
               
               
                 4 
                 9 
                 0.00065 
                 10 
                 0.000090 
                 15 
                 0.00009 
               
               
                 4 
                 9 
                 0.00026 
                 10 
                 0.000036 
                 15 
                 0.00003 
               
               
                 4 
                 9 
                 0.00013 
                 10 
                 0.000018 
                 15 
                 0.00002 
               
               
                 8 
                 2 
                 0.0018 
                 9 
                 0.001300 
                 10 
                 0.00018 
               
               
                 8 
                 2 
                 0.0009 
                 9 
                 0.000650 
                 10 
                 0.00180 
               
               
                 8 
                 2 
                 0.00036 
                 9 
                 0.000260 
                 10 
                 0.01800 
               
               
                 8 
                 2 
                 0.00018 
                 9 
                 0.000130 
                 10 
                 0.00002 
               
               
                 9 
                 2 
                 0.0018 
                 9 
                 0.001300 
                 19 
                 0.00010 
               
               
                 9 
                 2 
                 0.0009 
                 9 
                 0.000650 
                 19 
                 0.00005 
               
               
                 9 
                 2 
                 0.00036 
                 9 
                 0.000260 
                 19 
                 0.00002 
               
               
                 9 
                 2 
                 0.00018 
                 9 
                 0.000130 
                 19 
                 0.00001 
               
               
                   
               
             
          
         
       
     
         [0421]      FIGS. 11-15  show the results of the ABEL® antioxidant assay for peroxynitrite. 
       Example 31 
     Tables Showing ABEL-RAC Scores for Combinations of Three Raw Materials Compared to the Sum of the Abel-Rac Scores for Each Individual Raw Material, and Calculated Synergy Values 
       [0422]      
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Combination 1 
               
             
          
           
               
                   
                 EC50 
                   
                   
                   
               
               
                   
                 mg/mL of 
                 ABEL-RAC 
                 Percentage of 
                 ABEL-RAC 
               
               
                   
                 pure 
                 peroxynitrite of 
                 ingredient in 
                 contribution in 
               
               
                 Ingredient 
                 ingredient 
                 pure ingredient 
                 combination 
                 combination 
               
               
                   
               
             
          
           
               
                 RM10 
                 0.0018 
                 88053 
                 40 
                 35221 
               
               
                 RM15 
                 0.0017 
                 106837 
                 38 
                 40361 
               
               
                 RM19 
                 0.0010 
                 799816 
                 22 
                 177737 
               
             
          
           
               
                 SUM 
                 253319 
               
               
                 ABEL-RAC PEROXYNITRITE-COMBINATION 1 
                 396562 
               
               
                 SYNERGY 
                 1.57 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Combination 3 
               
             
          
           
               
                   
                 EC50 
                   
                   
                   
               
               
                   
                 mg/mL of 
                 ABEL-RAC 
                 Percentage of 
                 ABEL-RAC 
               
               
                   
                 pure 
                 peroxynitrite of 
                 ingredient in 
                 contribution in 
               
               
                 Ingredient 
                 ingredient 
                 pure ingredient 
                 combination 
                 combination 
               
               
                   
               
             
          
           
               
                 RM9 
                 0.0130 
                 78317 
                 82 
                 64438 
               
               
                 RM10 
                 0.0018 
                 88053 
                 11 
                 10031 
               
               
                 RM19 
                 0.0010 
                 759825 
                 6 
                 45590 
               
             
          
           
               
                 SUM 
                 120059 
               
               
                 ABEL-RAC PEROXYNITRITE-COMBINATION 3 
                 119470 
               
               
                 SYNERGY 
                 1.00 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Combination 4 
               
             
          
           
               
                   
                 EC50 
                   
                   
                   
               
               
                   
                 mg/mL of 
                 ABEL-RAC 
                 Percentage of 
                 ABEL-RAC 
               
               
                   
                 pure 
                 peroxynitrite of 
                 ingredient in 
                 contribution in 
               
               
                 Ingredient 
                 ingredient 
                 pure ingredient 
                 combination 
                 combination 
               
               
                   
               
             
          
           
               
                 RM9 
                 0.0130 
                 78317 
                 79 
                 61704 
               
               
                 RM10 
                 0.0018 
                 88053 
                 11 
                 9606 
               
               
                 RM15 
                 0.0017 
                 101495 
                 10 
                 10457 
               
             
          
           
               
                 SUM 
                 81767 
               
               
                 ABEL-RAC PEROXYNITRITE-COMBINATION 4 
                 111499 
               
               
                 SYNERGY 
                 1.36 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 Combination 8 
               
             
          
           
               
                   
                 EC50 
                   
                   
                   
               
               
                   
                 mg/mL of 
                 ABEL-RAC 
                 Percentage of 
                 ABEL-RAC 
               
               
                   
                 pure 
                 peroxynitrite of 
                 ingredient in 
                 contribution in 
               
               
                 Ingredient 
                 ingredient 
                 pure ingredient 
                 combination 
                 combination 
               
               
                   
               
             
          
           
               
                 RM2 
                 0.0180 
                 72807 
                 55 
                 39955 
               
               
                 RM9 
                 0.0130 
                 78317 
                 40 
                 31327 
               
               
                 RM10 
                 0.0018 
                 88053 
                 5 
                 4832 
               
             
          
           
               
                 SUM 
                 76114 
               
               
                 ABEL-RAC PEROXYNITRITE-COMBINATION 8 
                 111499 
               
               
                 SYNERGY 
                 1.46 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 Combination 9 
               
             
          
           
               
                   
                 EC50 
                   
                   
                   
               
               
                   
                 mg/mL of 
                 ABEL-RAC 
                 Percentage of 
                 ABEL-RAC 
               
               
                   
                 pure 
                 peroxynitrite of 
                 ingredient in 
                 contribution in 
               
               
                 Ingredient 
                 ingredient 
                 pure ingredient 
                 combination 
                 combination 
               
               
                   
               
             
          
           
               
                 RM2 
                 0.0180 
                 72807 
                 56 
                 40954 
               
               
                 RM9 
                 0.0130 
                 78317 
                 41 
                 31816 
               
               
                 RM19 
                 0.0010 
                 759825 
                 3 
                 23745 
               
             
          
           
               
                 SUM 
                 96515 
               
               
                 ABEL-RAC PEROXYNITRITE-COMBINATION 9 
                 125816 
               
               
                 SYNERGY 
                 1.30 
               
               
                   
               
             
          
         
       
     
       Example 32 
     Antioxidant Testing for Combinations of Three Antioxidant Agents Selected from List A in a Cream Base 
       [0000]    
       
         1. The raw materials were diluted with solvent as detailed in Table 1, to concentrations one hundred times stronger that the target concentration in the cream. Table 9 shows the concentrations of the raw materials that were used for each of the three combinations tested. 
         2. 100μL of each raw material (ie 300 μL in total) was added to 9.7 g of the base cream. (It was assumed that the density of the cream was equivalent to the density of water). The cream was stirred gently with a flat plastics stick ensuring homogeneity before making further dilutions. For the cream control, 300 μL solvent (without raw materials) was added to 9.7 g of the cream base. 
         3. The cream combinations and the cream control were diluted with assay buffer (PBS) in the same ratio to produce the following cream concentrations: 4 mg/mL, 10 mg/mL, 20 mg/mL and 100 mg/mL. Thus, the working concentration used was based on the cream concentration, and the ratio between base cream and raw material is kept constant. 
         4. In the antioxidant assay with peroxynitrite, 100 μL of diluted cream was added to a well of a microplate, the final volume of the assay being 200 μL. 
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 Concentrations of raw materials used in each cream combination 
               
             
          
           
               
                   
                   
                   
                 Stock 
               
               
                 Cream 
                   
                 Target 
                 concentration of 
               
               
                 combination 
                   
                 concentration in 
                 RM in solvent 
               
               
                 number 
                 RM number 
                 cream (%) 
                 mg/ml 
               
               
                   
               
             
          
           
               
                 1 
                 10 
                 0.0018 
                 18 
               
               
                   
                 15 
                 0.0017 
                 17 
               
               
                   
                 19 
                 0.0010 
                 10 
               
               
                 8 
                 2 
                 0.018 
                 180 
               
               
                   
                 9 
                 0.013 
                 130 
               
               
                   
                 10 
                 0.0018 
                 18 
               
               
                 8 modified 
                 2 
                 0.0018 
                 18 
               
               
                   
                 9 
                 0.013 
                 130 
               
               
                   
                 10 
                 0.018 
                 180 
               
               
                   
               
             
          
         
       
     
         [0427]      FIGS. 16-18  show the results of the ABEL® antioxidant assay for peroxynitrite, for the base cream containing raw material combinations 1, 8 and 8 modified. 
         [0428]    The results of the ABEL® antioxidant assay for peroxynitrite for the cream containing raw material combinations 1, 8 and 8 mod where adjusted to take account of the assay result for the cream control (ie base cream with solvent, but no raw material). The ABEL-RAC scores were then calculated, and are shown in Table 10. The ABEL-RAC scores thus provide measure of the antioxidant effect of the combination of antioxidant agents in the cream—excluding any antioxidant effects shown by the base cream itself. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 ABEL-RAC scores for three cream combinations 
               
             
          
           
               
                   
                 Cream combination 
                   
                   
               
               
                   
                 number 
                 EC 50  (mg) 
                 ABEL-RAC 
               
               
                   
                   
               
             
          
           
               
                   
                 1 
                 5.0575 
                 20 
               
               
                   
                 8 
                 3.1703 
                 32 
               
               
                   
                 8 modified 
                 2.2898 
                 44 
               
               
                   
                   
               
             
          
         
       
     
         [0429]    The base cream (without solvent or raw material combinations) was also tested, and the results were compared to the results of the cream control (base cream with solvent, no raw material). The result showed that the solvents (water and IMS) only have a minor effect on the peroxynitrite assay. 
       Example 33 
     Comparison Between the Use of Industrial Methylated Spirits (IMS) and Ethanol as Solvent 
       [0430]    IMS was the alcohol used as solvent in the initial ABEL® testing. However, ethanol would be used to dilute the relevant raw materials before addition into cosmetic formulations, rather than IMS. The ABEL® peroxynitrite assay for peroxynitrite was run for dimethylmethoxy chromanol diluted with IMS and dimethylmethoxy chromanol diluted with ethanol. The results, shown in  FIG. 19 , demonstrate that the use of ethanol rather than IMS does not make an appreciable difference. Hence, the results of assays in which ethanol was used as a solvent are considered to be comparable to the results of assays in which IMS was used as a solvent. 
       Example 34 
     Antioxidant Testing for a Further Combination of Three Antioxidant Agents from List A 
       [0431]    Raw material combination 12 comprises raw materials 2, 9 and 10 in the ratio 2:15:2. 
         [0432]    The testing method was as described in Example 15, except the relevant raw materials were diluted with ethanol rather than IMS as indicated in Table 11. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 11 
               
             
             
               
                   
               
               
                 Raw materials and solvents used 
               
             
          
           
               
                   
                   
                 RM 
                   
               
               
                   
                 RM name 
                 number 
                 Solvent 
               
               
                   
                   
               
             
          
           
               
                   
                 ginkgo extract 
                 2 
                 1:1 ethanol:water 
               
               
                   
                 emblica extract 
                 9 
                 water 
               
               
                   
                 dimethylmethoxy chromanol 
                 10 
                 ethanol 
               
               
                   
                   
               
             
          
         
       
     
         [0433]    The concentrations of each raw material in cream combination 12 are indicated in Table 12 below. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 12 
               
             
             
               
                   
               
               
                 Concentration of raw materials used in cream combination 12 
               
             
          
           
               
                   
                   
                   
                 Stock 
               
               
                 Cream 
                   
                 Target 
                 concentration of 
               
               
                 combination 
                   
                 concentration in 
                 RM in solvent 
               
               
                 number 
                 RM number 
                 cream (%) 
                 mg/mL 
               
               
                   
               
             
          
           
               
                 12 
                 2 
                 0.02 
                 20 
               
               
                   
                 9 
                 0.015 
                 15 
               
               
                   
                 10 
                 0.02 
                 20 
               
               
                   
               
             
          
         
       
     
         [0434]    The cream concentrations that were tested were 1 mg/mL, 5 mg/mL, 10 mg/mL, 25 mg/mL and 50 mg/mL. 
         [0435]    The results of the peroxynitrite assay for cream combination 12 are shown in  FIG. 20 . 
         [0436]    The results were adjusted to take account of the assay result for the cream control (ie base cream with solvent, but no raw materials), and the ABEL-RAC score calculated. The score is shown in Table 13, and it indicates the antioxidant effect of the combination of antioxidant agents in the cream (excluding any antioxidant effects shown by the base cream itself). 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 13 
               
             
             
               
                   
               
               
                 ABEL-RAC score for cream combination 12 
               
             
          
           
               
                 Cream combination 
                   
                 ABEL- 
               
               
                 number 
                 EC 50  (mg) 
                 RAC 
               
               
                   
               
               
                 12 
                 2.4268 
                 41