Abstract:
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is related to U.S. Provisional Application No. 61/756,973, filed Jan. 25,2013, the entire disclosure of which is hereby incorporated by reference in its entirety for all purposes. 
    
    
     SEQUENCE LISTING 
     The instant application contains a Sequence Listing which has been submitted via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jan. 31,2014, is named 25951US_sequencelisting.txt, and is 90,236 bytes in size. 
     BACKGROUND 
     Many existing photoautotrophic organisms (i.e., plants, algae, and photosynthetic bacteria) are poorly suited for industrial bioprocessing and have therefore not demonstrated commercial viability. Recombinant photosynthetic microorganisms have been engineered to produce hydrocarbons and alcohols in amounts that exceed the levels produced naturally by the organism. 
     SUMMARY 
     Described herein is an engineered microorganism, wherein said engineered microorganism comprises one or more recombinant genes encoding one or more enzymes having enzyme activities which catalyze the production of medium chain-length alkanes, wherein the enzyme activities comprise: an alkane deformylative monooxygenase activity, a thioesterase activity, a carboxylic acid reductase activity, and a phosphopanthetheinyl transferase activity; and/or an alkane deformylative monooxygenase activity, a thioesterase activity, a long-chain fatty acid CoA-ligase activity, and a long-chain acyl-CoA reductase activity. 
     In some aspects, the enzymes comprise an alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, and a phosphopanthetheinyl transferase. In some aspects, the alkane deformylative monooxygenase has EC number 4.1.99.5, the thioesterase has EC number 3.1.2.14, the carboxylic acid reductase has EC number 1.2.99.6, and the phosphopanthetheinyl transferase has EC number 2.7.8.7. In some aspects, the alkane deformylative monooxygenase is encoded by adm, the thioesterase is encoded by fatB or fatB2, the carboxylic acid reductase is encoded by carB, and the phosphopanthetheinyl transferase is encoded by entD. 
     In some aspects, the enzyme having alkane deformylative monooxygenase activity has EC number 4.1.99.5. In some aspects, the enzyme having thioesterase activity has EC number 3.1.2.14. In some aspects, the enzyme having carboxylic acid reductase activity has EC number 1.2.99.6. In some aspects, the enzyme having phosphopanthetheinyl transferase activity has EC number 2.7.8.7. 
     In some aspects, the enzymes comprise an alkane deformylative monooxygenase, a thioesterase, a long-chain fatty acid CoA-ligase, and a long-chain acyl-CoA reductase. In some aspects, the alkane deformylative monooxygenase has EC number 4.1.99.5, the thioesterase has EC number 3.1.2.14, the long-chain fatty acid CoA-ligase has EC number 6.2.1.3, and the long-chain acyl-CoA reductase has EC number 1.2.1.50. In some aspects, the alkane deformylative monooxygenase is encoded by adm, the thioesterase is encoded by fatB or fatB2, the long-chain fatty acid CoA-ligase is encoded by fadD, and the long-chain acyl-CoA reductase is encoded by acrM. 
     In some aspects, the enzyme having alkane deformylative monooxygenase activity has EC number 4.1.99.5. In some aspects, the enzyme having thioesterase activity has EC number 3.1.2.14. In some aspects, the enzyme having long-chain fatty acid CoA-ligase activity has EC number 6.2.1.3. In some aspects, the enzyme having long-chain acyl-CoA reductase activity has EC number 1.2.1.50. 
     In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a thioesterase that catalyzes the conversion of acyl-ACP to a fatty acid. In some aspects, the one or more recombinant genes comprises a recombinant gene encoding a phosphopanthetheinyl transferase that phosphopatetheinylates the ACP moiety of a protein encoded by a carboxylic acid reductase gene. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a carboxylic acid reductase that catalyzes the conversion of fatty acid to fatty aldehyde. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a alkane deformylative monooxygenase that catalyzes the conversion of fatty aldehyde to an alkane or alkene. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a fatty acid CoA-ligase that catalyzes the conversion of fatty acid to acyl-CoA. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding an acyl-CoA reductase that catalyzes the conversion of acyl-CoA to fatty aldehyde. 
     In some aspects, said microorganism is a bacterium. In some aspects, said microorganism is a gram-negative bacterium. In some aspects, said microorganism is  E. coli.    
     In some aspects, said microorganism is a photosynthetic microorganism. In some aspects, said microorganism is a cyanobacterium. In some aspects, said microorganism is a thermotolerant cyanobacterium. In some aspects, said microorganism is a Synechococcus species. 
     In some aspects, expression of an operon comprising the one or more recombinant genes is controlled by a recombinant promoter, and wherein the promoter is constitutive or inducible. In some aspects, said operon is integrated into the genome of said microorganism. In some aspects, said operon is extrachromosomal. 
     In some aspects, said medium chain-length alkanes are less than or equal to 11 carbon atoms in length. In some aspects, said medium chain-length alkanes are 7 to 11 carbon atoms in length. In some aspects, said medium chain-length alkanes are 7, 8, 9, 10, or 11 carbon atoms in length. 
     In some aspects, said recombinant genes are at least 90% or at least 95% identical to a sequence shown in the Tables. 
     Also described herein is a cell culture comprising a culture medium and a microorganism described herein. 
     Also described herein is a method for producing hydrocarbons, comprising: culturing an engineered microorganism described herein in a culture medium, wherein said engineered microorganism produces increased amounts of medium chain-length alkanes relative to an otherwise identical microorganism, cultured under identical conditions, but lacking said recombinant genes. In some aspects, the method further includes allowing medium chain-length alkanes to accumulate in the culture medium or in the organism. In some aspects, the method further includes isolating at least a portion of the medium chain-length alkanes. In some aspects, the method further includes processing the isolated medium chain-length alkanes to produce a processed material. 
     Also described herein is a method for producing hydrocarbons, comprising: (i) culturing an engineered microorganism described herein in a culture medium; and (ii) exposing said engineered microorganism to light and inorganic carbon, wherein said exposure results in the conversion of said inorganic carbon by said microorganism into medium chain-length alkanes, wherein said medium chain-length alkanes are produced in an amount greater than that produced by an otherwise identical microorganism, cultured under identical conditions, but lacking said recombinant genes. In some aspects, the method further includes allowing medium chain-length alkanes to accumulate in the culture medium or in the organism. In some aspects, the method further includes isolating at least a portion of the medium chain-length alkanes. In some aspects, the method further includes processing the isolated medium chain-length alkanes to produce a processed material. 
     Also described herein is a composition comprising medium chain-length alkanes, wherein said medium chain-length alkanes are produced by a method described herein. In some aspects, the composition comprises at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 99% medium chain-length alkanes. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1 . SDS-PAGE gel showing the overexpression of AcrM protein in  E. coli.    
         FIG. 2 . TIC chromatograms of assays with (A) decanoyl-CoA, (B) lauroyl-CoA. Solid line: wild type BL21(DE3); dotted line: acrM-expressing BL21(DE3). 
         FIG. 3 . GC/FID chromatogram showing the detection of C13 and C15 alkanes produced by  Synechococcus  sp. PCC 7002 strain expressing Adm, CarB, TesA and EntD proteins. Grey trace: control strain (does not express CarB protein); solid black trace: Standards of C13, C14, and C15 n-alkanes; dashed black trace:  Synechococcus  sp. PCC 7002 strain expressing Adm, CarB, TesA, and EntD proteins. 
         FIG. 4 . TIC chromatograms of samples from acid-fed (dashed lines) or control (solid lines)  Synechococcus  sp. PCC 7002 expressing Adm and CarB. A and D: octanoic acid feeding, B and E: decanoic acid feeding, C and F: dodecanoic acid feeding. 
         FIG. 5 . GC/FID chromatogram showing the detection of nonane produced by  Synechococcus  sp. PCC 7002 strain expressing Adm, CarB, FatB2 and EntD proteins at 12 h and 72 h. Solid trace: control strain (wild type); dotted trace:  Synechococcus  sp. PCC 7002 strain expressing Adm, CarB, FatB2, and EntD proteins. 
         FIG. 6 . Examples of pathways for production of medium chain-length alkanes. Note that the use of carB can be facilitated by the product of entD (phosphopatetheinyl transferase), which phosphopatetheinylates the ACP moiety of the CarB protein. For example, one can use the  Bacillus  entD, whose enzyme product has a wide substrate spectrum that includes CarB. 
     
    
    
     DETAILED DESCRIPTION 
     Unless otherwise defined herein, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include the plural and plural terms shall include the singular. Generally, nomenclatures used in connection with, and techniques of, biochemistry, enzymology, molecular and cellular biology, microbiology, genetics and protein and nucleic acid chemistry and hybridization described herein are those well known and commonly used in the art. 
     The methods and techniques of the present invention are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification unless otherwise indicated. See, e.g., Sambrook et al.,  Molecular Cloning: A Laboratory Manual,  2d ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989); Ausubel et al.,  Current Protocols in Molecular Biology , Greene Publishing Associates (1992, and Supplements to 2002); Harlow and Lane,  Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1990); Taylor and Drickamer,  Introduction to Glycobiology , Oxford Univ. Press (2003);  Worthington Enzyme Manual , Worthington Biochemical Corp., Freehold, N.J.;  Handbook of Biochemistry: Section A Proteins , Vol I, CRC Press (1976); Handbook of Biochemistry: Section A Proteins, Vol II, CRC Press (1976);  Essentials of Glycobiology , Cold Spring Harbor Laboratory Press (1999). 
     All publications, patents and other references mentioned herein are hereby incorporated by reference in their entireties. 
     The following terms, unless otherwise indicated, shall be understood to have the following meanings: 
     The term “polynucleotide” or “nucleic acid molecule” refers to a polymeric form of nucleotides of at least 10 bases in length. The term includes DNA molecules (e.g., cDNA or genomic or synthetic DNA) and RNA molecules (e.g., mRNA or synthetic RNA), as well as analogs of DNA or RNA containing non-natural nucleotide analogs, non-native internucleoside bonds, or both. The nucleic acid can be in any topological conformation. For instance, the nucleic acid can be single-stranded, double-stranded, triple-stranded, quadruplexed, partially double-stranded, branched, hairpinned, circular, or in a padlocked conformation. 
     Unless otherwise indicated, and as an example for all sequences described herein under the general format “SEQ ID NO:”, “nucleic acid comprising SEQ ID NO:1” refers to a nucleic acid, at least a portion of which has either (i) the sequence of SEQ ID NO:1, or (ii) a sequence complementary to SEQ ID NO:1. The choice between the two is dictated by the context. For instance, if the nucleic acid is used as a probe, the choice between the two is dictated by the requirement that the probe be complementary to the desired target. 
     An “isolated” RNA, DNA or a mixed polymer is one which is substantially separated from other cellular components that naturally accompany the native polynucleotide in its natural host cell, e.g., ribosomes, polymerases and genomic sequences with which it is naturally associated. 
     As used herein, an “isolated” organic molecule (e.g., an alkane) is one which is substantially separated from the cellular components (membrane lipids, chromosomes, proteins) of the host cell from which it originated, or from the medium in which the host cell was cultured. The term does not require that the biomolecule has been separated from all other chemicals, although certain isolated biomolecules may be purified to near homogeneity. 
     The term “recombinant” refers to a biomolecule, e.g., a gene or protein, that (1) has been removed from its naturally occurring environment, (2) is not associated with all or a portion of a polynucleotide in which the gene is found in nature, (3) is operatively linked to a polynucleotide which it is not linked to in nature, or (4) does not occur in nature. The term “recombinant” can be used in reference to cloned DNA isolates, chemically synthesized polynucleotide analogs, or polynucleotide analogs that are biologically synthesized by heterologous systems, as well as proteins and/or mRNAs encoded by such nucleic acids. 
     As used herein, an endogenous nucleic acid sequence in the genome of an organism (or the encoded protein product of that sequence) is deemed “recombinant” herein if a heterologous sequence is placed adjacent to the endogenous nucleic acid sequence, such that the expression of this endogenous nucleic acid sequence is altered. In this context, a heterologous sequence is a sequence that is not naturally adjacent to the endogenous nucleic acid sequence, whether or not the heterologous sequence is itself endogenous (originating from the same host cell or progeny thereof) or exogenous (originating from a different host cell or progeny thereof). By way of example, a promoter sequence can be substituted (e.g., by homologous recombination) for the native promoter of a gene in the genome of a host cell, such that this gene has an altered expression pattern. This gene would now become “recombinant” because it is separated from at least some of the sequences that naturally flank it. 
     A nucleic acid is also considered “recombinant” if it contains any modifications that do not naturally occur to the corresponding nucleic acid in a genome. For instance, an endogenous coding sequence is considered “recombinant” if it contains an insertion, deletion or a point mutation introduced artificially, e.g., by human intervention. A “recombinant nucleic acid” also includes a nucleic acid integrated into a host cell chromosome at a heterologous site and a nucleic acid construct present as an episome. 
     As used herein, the phrase “degenerate variant” of a reference nucleic acid sequence encompasses nucleic acid sequences that can be translated, according to the standard genetic code, to provide an amino acid sequence identical to that translated from the reference nucleic acid sequence. The term “degenerate oligonucleotide” or “degenerate primer” is used to signify an oligonucleotide capable of hybridizing with target nucleic acid sequences that are not necessarily identical in sequence but that are homologous to one another within one or more particular segments. 
     The term “percent sequence identity” or “identical” in the context of nucleic acid sequences refers to the residues in the two sequences which are the same when aligned for maximum correspondence. The length of sequence identity comparison may be over a stretch of at least about nine nucleotides, usually at least about 20 nucleotides, more usually at least about 24 nucleotides, typically at least about 28 nucleotides, more typically at least about 32 nucleotides, and preferably at least about 36 or more nucleotides. There are a number of different algorithms known in the art which can be used to measure nucleotide sequence identity. For instance, polynucleotide sequences can be compared using FASTA, Gap or Bestfit, which are programs in Wisconsin Package Version 10.0, Genetics Computer Group (GCG), Madison, Wis. FASTA provides alignments and percent sequence identity of the regions of the best overlap between the query and search sequences. Pearson, Methods Enzymol. 183:63-98 (1990) (hereby incorporated by reference in its entirety). For instance, percent sequence identity between nucleic acid sequences can be determined using FASTA with its default parameters (a word size of 6 and the NOPAM factor for the scoring matrix) or using Gap with its default parameters as provided in GCG Version 6.1, herein incorporated by reference. Alternatively, sequences can be compared using the computer program, BLAST (Altschul et al.,  J. Mol. Biol.  215:403-410 (1990); Gish and States,  Nature Genet.  3:266-272 (1993); Madden et al.,  Meth. Enzymol.  266:131-141 (1996); Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997); Zhang and Madden,  Genome Res.  7:649-656 (1997)), especially blastp or tblastn (Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997)). 
     The term “substantial homology” or “substantial similarity,” when referring to a nucleic acid or fragment thereof, indicates that, when optimally aligned with appropriate nucleotide insertions or deletions with another nucleic acid (or its complementary strand), there is nucleotide sequence identity in at least about 76%, 80%, 85%, preferably at least about 90%, and more preferably at least about 95%, 96%, 97%, 98% or 99% of the nucleotide bases, as measured by any well-known algorithm of sequence identity, such as FASTA, BLAST or Gap, as discussed above. 
     Alternatively, substantial homology or similarity exists when a nucleic acid or fragment thereof hybridizes to another nucleic acid, to a strand of another nucleic acid, or to the complementary strand thereof, under stringent hybridization conditions. “Stringent hybridization conditions” and “stringent wash conditions” in the context of nucleic acid hybridization experiments depend upon a number of different physical parameters. Nucleic acid hybridization will be affected by such conditions as salt concentration, temperature, solvents, the base composition of the hybridizing species, length of the complementary regions, and the number of nucleotide base mismatches between the hybridizing nucleic acids, as will be readily appreciated by those skilled in the art. One having ordinary skill in the art knows how to vary these parameters to achieve a particular stringency of hybridization. 
     In general, “stringent hybridization” is performed at about 25° C. below the thermal melting point (T m ) for the specific DNA hybrid under a particular set of conditions. “Stringent washing” is performed at temperatures about 5° C. lower than the T m  for the specific DNA hybrid under a particular set of conditions. The T m  is the temperature at which 50% of the target sequence hybridizes to a perfectly matched probe. See Sambrook et al., Molecular Cloning: A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989), page 9.51, hereby incorporated by reference. For purposes herein, “stringent conditions” are defined for solution phase hybridization as aqueous hybridization (i.e., free of formamide) in 6×SSC (where 20×SSC contains 3.0 M NaCl and 0.3 M sodium citrate), 1% SDS at 65° C. for 8-12 hours, followed by two washes in 0.2×SSC, 0.1% SDS at 65° C. for 20 minutes. It will be appreciated by the skilled worker that hybridization at 65° C. will occur at different rates depending on a number of factors including the length and percent identity of the sequences which are hybridizing. 
     The nucleic acids (also referred to as polynucleotides) of this present invention may include both sense and antisense strands of RNA, cDNA, genomic DNA, and synthetic forms and mixed polymers of the above. They may be modified chemically or biochemically or may contain non-natural or derivatized nucleotide bases, as will be readily appreciated by those of skill in the art. Such modifications include, for example, labels, methylation, substitution of one or more of the naturally occurring nucleotides with an analog, internucleotide modifications such as uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates, etc.), charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), pendent moieties (e.g., polypeptides), intercalators (e.g., acridine, psoralen, etc.), chelators, alkylators, and modified linkages (e.g., alpha anomeric nucleic acids, etc.) Also included are synthetic molecules that mimic polynucleotides in their ability to bind to a designated sequence via hydrogen bonding and other chemical interactions. Such molecules are known in the art and include, for example, those in which peptide linkages substitute for phosphate linkages in the backbone of the molecule. Other modifications can include, for example, analogs in which the ribose ring contains a bridging moiety or other structure such as the modifications found in “locked” nucleic acids. 
     The term “mutated” when applied to nucleic acid sequences means that nucleotides in a nucleic acid sequence may be inserted, deleted or changed compared to a reference nucleic acid sequence. A single alteration may be made at a locus (a point mutation) or multiple nucleotides may be inserted, deleted or changed at a single locus. In addition, one or more alterations may be made at any number of loci within a nucleic acid sequence. A nucleic acid sequence may be mutated by any method known in the art including but not limited to mutagenesis techniques such as “error-prone PCR” (a process for performing PCR under conditions where the copying fidelity of the DNA polymerase is low, such that a high rate of point mutations is obtained along the entire length of the PCR product; see, e.g., Leung et al., Technique, 1:11-15 (1989) and Caldwell and Joyce, PCR Methods Applic. 2:28-33 (1992)); and “oligonucleotide-directed mutagenesis” (a process which enables the generation of site-specific mutations in any cloned DNA segment of interest; see, e.g., Reidhaar-Olson and Sauer, Science 241:53-57 (1988)). 
     The term “attenuate” as used herein generally refers to a functional deletion, including a mutation, partial or complete deletion, insertion, or other variation made to a gene sequence or a sequence controlling the transcription of a gene sequence, which reduces or inhibits production of the gene product, or renders the gene product non-functional. In some instances a functional deletion is described as a knockout mutation. Attenuation also includes amino acid sequence changes by altering the nucleic acid sequence, placing the gene under the control of a less active promoter, down-regulation, expressing interfering RNA, ribozymes or antisense sequences that target the gene of interest, or through any other technique known in the art. In one example, the sensitivity of a particular enzyme to feedback inhibition or inhibition caused by a composition that is not a product or a reactant (non-pathway specific feedback) is lessened such that the enzyme activity is not impacted by the presence of a compound. In other instances, an enzyme that has been altered to be less active can be referred to as attenuated. 
     Deletion: The removal of one or more nucleotides from a nucleic acid molecule or one or more amino acids from a protein, the regions on either side being joined together. 
     Knock-out: A gene whose level of expression or activity has been reduced to zero. In some examples, a gene is knocked-out via deletion of some or all of its coding sequence. In other examples, a gene is knocked-out via introduction of one or more nucleotides into its open reading frame, which results in translation of a non-sense or otherwise non-functional protein product. 
     The term “vector” as used herein is intended to refer to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a “plasmid,” which generally refers to a circular double stranded DNA loop into which additional DNA segments may be ligated, but also includes linear double-stranded molecules such as those resulting from amplification by the polymerase chain reaction (PCR) or from treatment of a circular plasmid with a restriction enzyme. Other vectors include cosmids, bacterial artificial chromosomes (BAC) and yeast artificial chromosomes (YAC). Another type of vector is a viral vector, wherein additional DNA segments may be ligated into the viral genome (discussed in more detail below). Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., vectors having an origin of replication which functions in the host cell). Other vectors can be integrated into the genome of a host cell upon introduction into the host cell, and are thereby replicated along with the host genome. Moreover, certain preferred vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “recombinant expression vectors” (or simply “expression vectors”). 
     “Operatively linked” or “operably linked” expression control sequences refers to a linkage in which the expression control sequence is contiguous with the gene of interest to control the gene of interest, as well as expression control sequences that act in trans or at a distance to control the gene of interest. 
     The term “expression control sequence” as used herein refers to polynucleotide sequences which are necessary to affect the expression of coding sequences to which they are operatively linked. Expression control sequences are sequences which control the transcription, post-transcriptional events and translation of nucleic acid sequences. Expression control sequences include appropriate transcription initiation, termination, promoter and enhancer sequences; efficient RNA processing signals such as splicing and polyadenylation signals; sequences that stabilize cytoplasmic mRNA; sequences that enhance translation efficiency (e.g., ribosome binding sites); sequences that enhance protein stability; and when desired, sequences that enhance protein secretion. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include promoter, ribosomal binding site, and transcription termination sequence. The term “control sequences” is intended to include, at a minimum, all components whose presence is essential for expression, and can also include additional components whose presence is advantageous, for example, leader sequences and fusion partner sequences. 
     The term “recombinant host cell” (or simply “host cell”), as used herein, is intended to refer to a cell into which a recombinant vector has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell but to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein. A recombinant host cell may be an isolated cell or cell line grown in culture or may be a cell which resides in a living tissue or organism. 
     The term “peptide” as used herein refers to a short polypeptide, e.g., one that is typically less than about 50 amino acids long and more typically less than about 30 amino acids long. The term as used herein encompasses analogs and mimetics that mimic structural and thus biological function. 
     The term “polypeptide” encompasses both naturally-occurring and non-naturally-occurring proteins, and fragments, mutants, derivatives and analogs thereof. A polypeptide may be monomeric or polymeric. Further, a polypeptide may comprise a number of different domains each of which has one or more distinct activities. 
     The term “isolated protein” or “isolated polypeptide” is a protein or polypeptide that by virtue of its origin or source of derivation (1) is not associated with naturally associated components that accompany it in its native state, (2) exists in a purity not found in nature, where purity can be adjudged with respect to the presence of other cellular material (e.g., is free of other proteins from the same species) (3) is expressed by a cell from a different species, or (4) does not occur in nature (e.g., it is a fragment of a polypeptide found in nature or it includes amino acid analogs or derivatives not found in nature or linkages other than standard peptide bonds). Thus, a polypeptide that is chemically synthesized or synthesized in a cellular system different from the cell from which it naturally originates will be “isolated” from its naturally associated components. A polypeptide or protein may also be rendered substantially free of naturally associated components by isolation, using protein purification techniques well known in the art. As thus defined, “isolated” does not necessarily require that the protein, polypeptide, peptide or oligopeptide so described has been physically removed from its native environment. 
     The term “polypeptide fragment” as used herein refers to a polypeptide that has a deletion, e.g., an amino-terminal and/or carboxy-terminal deletion compared to a full-length polypeptide. In a preferred embodiment, the polypeptide fragment is a contiguous sequence in which the amino acid sequence of the fragment is identical to the corresponding positions in the naturally-occurring sequence. Fragments typically are at least 5, 6, 7, 8, 9 or 10 amino acids long, preferably at least 12, 14, 16 or 18 amino acids long, more preferably at least 20 amino acids long, more preferably at least 25, 30, 35, 40 or 45, amino acids, even more preferably at least 50 or 60 amino acids long, and even more preferably at least 70 amino acids long. 
     A “modified derivative” refers to polypeptides or fragments thereof that are substantially homologous in primary structural sequence but which include, e.g., in vivo or in vitro chemical and biochemical modifications or which incorporate amino acids that are not found in the native polypeptide. Such modifications include, for example, acetylation, carboxylation, phosphorylation, glycosylation, ubiquitination, labeling, e.g., with radionuclides, and various enzymatic modifications, as will be readily appreciated by those skilled in the art. A variety of methods for labeling polypeptides and of substituents or labels useful for such purposes are well known in the art, and include radioactive isotopes such as  125 I,  32 P,  35 S, and  3 H, ligands which bind to labeled antiligands (e.g., antibodies), fluorophores, chemiluminescent agents, enzymes, and antiligands which can serve as specific binding pair members for a labeled ligand. The choice of label depends on the sensitivity required, ease of conjugation with the primer, stability requirements, and available instrumentation. Methods for labeling polypeptides are well known in the art. See, e.g., Ausubel et al.,  Current Protocols in Molecular Biology , Greene Publishing Associates (1992, and Supplements to 2002) (hereby incorporated by reference). 
     The term “fusion protein” refers to a polypeptide comprising a polypeptide or fragment coupled to heterologous amino acid sequences. Fusion proteins are useful because they can be constructed to contain two or more desired functional elements from two or more different proteins. A fusion protein comprises at least 10 contiguous amino acids from a polypeptide of interest, more preferably at least 20 or 30 amino acids, even more preferably at least 40, 50 or 60 amino acids, yet more preferably at least 75, 100 or 125 amino acids. Fusions that include the entirety of the proteins of the present invention have particular utility. The heterologous polypeptide included within the fusion protein of the present invention is at least 6 amino acids in length, often at least 8 amino acids in length, and usefully at least 15, 20, and 25 amino acids in length. Fusions that include larger polypeptides, such as an IgG Fc region, and even entire proteins, such as the green fluorescent protein (“GFP”) chromophore-containing proteins, have particular utility. Fusion proteins can be produced recombinantly by constructing a nucleic acid sequence which encodes the polypeptide or a fragment thereof in frame with a nucleic acid sequence encoding a different protein or peptide and then expressing the fusion protein. Alternatively, a fusion protein can be produced chemically by crosslinking the polypeptide or a fragment thereof to another protein. 
     The term “non-peptide analog” refers to a compound with properties that are analogous to those of a reference polypeptide. A non-peptide compound may also be termed a “peptide mimetic” or a “peptidomimetic.” See, e.g., Jones, Amino Acid and Peptide Synthesis, Oxford University Press (1992); Jung,  Combinatorial Peptide and Nonpeptide Libraries: A Handbook , John Wiley (1997); Bodanszky et al.,  Peptide Chemistry—A Practical Textbook , Springer Verlag (1993);  Synthetic Peptides: A Users Guide , (Grant, ed., W. H. Freeman and Co., 1992); Evans et al.,  J. Med. Chem.  30:1229 (1987); Fauchere, J.  Adv. Drug Res.  15:29 (1986); Veber and Freidinger,  Trends Neurosci.,  8:392-396 (1985); and references sited in each of the above, which are incorporated herein by reference. Such compounds are often developed with the aid of computerized molecular modeling. Peptide mimetics that are structurally similar to useful peptides of the present invention may be used to produce an equivalent effect and are therefore envisioned to be part of the present invention. 
     A “polypeptide mutant” or “mutein” refers to a polypeptide whose sequence contains an insertion, duplication, deletion, rearrangement or substitution of one or more amino acids compared to the amino acid sequence of a native or wild-type protein. A mutein may have one or more amino acid point substitutions, in which a single amino acid at a position has been changed to another amino acid, one or more insertions and/or deletions, in which one or more amino acids are inserted or deleted, respectively, in the sequence of the naturally-occurring protein, and/or truncations of the amino acid sequence at either or both the amino or carboxy termini. A mutein may have the same but preferably has a different biological activity compared to the naturally-occurring protein. 
     A mutein has at least 85% overall sequence homology to its wild-type counterpart. Even more preferred are muteins having at least 90% overall sequence homology to the wild-type protein. 
     In an even more preferred embodiment, a mutein exhibits at least 95% sequence identity, even more preferably 98%, even more preferably 99% and even more preferably 99.9% overall sequence identity. 
     Sequence homology may be measured by any common sequence analysis algorithm, such as Gap or Bestfit. 
     Amino acid substitutions can include those which: (1) reduce susceptibility to proteolysis, (2) reduce susceptibility to oxidation, (3) alter binding affinity for forming protein complexes, (4) alter binding affinity or enzymatic activity, and (5) confer or modify other physicochemical or functional properties of such analogs. 
     As used herein, the twenty conventional amino acids and their abbreviations follow conventional usage. See  Immunology—A Synthesis  (Golub and Gren eds., Sinauer Associates, Sunderland, Mass., 2 nd  ed. 1991), which is incorporated herein by reference. Stereoisomers (e.g., D-amino acids) of the twenty conventional amino acids, unnatural amino acids such as α-, α-disubstituted amino acids, N-alkyl amino acids, and other unconventional amino acids may also be suitable components for polypeptides of the present invention. Examples of unconventional amino acids include: 4-hydroxyproline, γ-carboxyglutamate, ε-N,N,N-trimethyllysine, ε-N-acetyllysine, O-phosphoserine, N-acetylserine, N-formylmethionine, 3-methylhistidine, 5-hydroxylysine, N-methylarginine, and other similar amino acids and imino acids (e.g., 4-hydroxyproline). In the polypeptide notation used herein, the left-hand end corresponds to the amino terminal end and the right-hand end corresponds to the carboxy-terminal end, in accordance with standard usage and convention. 
     A protein has “homology” or is “homologous” to a second protein if the nucleic acid sequence that encodes the protein has a similar sequence to the nucleic acid sequence that encodes the second protein. Alternatively, a protein has homology to a second protein if the two proteins have “similar” amino acid sequences. (Thus, the term “homologous proteins” is defined to mean that the two proteins have similar amino acid sequences.) As used herein, homology between two regions of amino acid sequence (especially with respect to predicted structural similarities) is interpreted as implying similarity in function. 
     When “homologous” is used in reference to proteins or peptides, it is recognized that residue positions that are not identical often differ by conservative amino acid substitutions. A “conservative amino acid substitution” is one in which an amino acid residue is substituted by another amino acid residue having a side chain (R group) with similar chemical properties (e.g., charge or hydrophobicity). In general, a conservative amino acid substitution will not substantially change the functional properties of a protein. In cases where two or more amino acid sequences differ from each other by conservative substitutions, the percent sequence identity or degree of homology may be adjusted upwards to correct for the conservative nature of the substitution. Means for making this adjustment are well known to those of skill in the art. See, e.g., Pearson, 1994 , Methods Mol. Biol.  24:307-31 and 25:365-89 (herein incorporated by reference). 
     The following six groups each contain amino acids that are conservative substitutions for one another: 1) Serine (S), Threonine (T); 2) Aspartic Acid (D), Glutamic Acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Alanine (A), Valine (V), and 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W). 
     Sequence homology for polypeptides, which is also referred to as percent sequence identity, is typically measured using sequence analysis software. See, e.g., the Sequence Analysis Software Package of the Genetics Computer Group (GCG), University of Wisconsin Biotechnology Center, 910 University Avenue, Madison, Wis. 53705. Protein analysis software matches similar sequences using a measure of homology assigned to various substitutions, deletions and other modifications, including conservative amino acid substitutions. For instance, GCG contains programs such as “Gap” and “Bestfit” which can be used with default parameters to determine sequence homology or sequence identity between closely related polypeptides, such as homologous polypeptides from different species of organisms or between a wild-type protein and a mutein thereof. See, e.g., GCG Version 6.1. 
     A preferred algorithm when comparing a particular polypeptide sequence to a database containing a large number of sequences from different organisms is the computer program BLAST (Altschul et al.,  J. Mol. Biol.  215:403-410 (1990); Gish and States,  Nature Genet.  3:266-272 (1993); Madden et al.,  Meth. Enzymol.  266:131-141 (1996); Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997); Zhang and Madden,  Genome Res.  7:649-656 (1997)), especially blastp or tblastn (Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997)). 
     Preferred parameters for BLASTp are: Expectation value: 10 (default); Filter: seg (default); Cost to open a gap: 11 (default); Cost to extend a gap: 1 (default); Max. alignments: 100 (default); Word size: 11 (default); No. of descriptions: 100 (default); Penalty Matrix: BLOWSUM62. 
     The length of polypeptide sequences compared for homology will generally be at least about 16 amino acid residues, usually at least about 20 residues, more usually at least about 24 residues, typically at least about 28 residues, and preferably more than about 35 residues. When searching a database containing sequences from a large number of different organisms, it is preferable to compare amino acid sequences. Database searching using amino acid sequences can be measured by algorithms other than blastp known in the art. For instance, polypeptide sequences can be compared using FASTA, a program in GCG Version 6.1. FASTA provides alignments and percent sequence identity of the regions of the best overlap between the query and search sequences. Pearson,  Methods Enzymol.  183:63-98 (1990) (incorporated by reference herein). For example, percent sequence identity between amino acid sequences can be determined using FASTA with its default parameters (a word size of 2 and the PAM250 scoring matrix), as provided in GCG Version 6.1, herein incorporated by reference. 
     “Specific binding” refers to the ability of two molecules to bind to each other in preference to binding to other molecules in the environment. Typically, “specific binding” discriminates over adventitious binding in a reaction by at least two-fold, more typically by at least 10-fold, often at least 100-fold. Typically, the affinity or avidity of a specific binding reaction, as quantified by a dissociation constant, is about 10 −7  M or stronger (e.g., about 10 −8  M, 10 −9  M or even stronger). 
     The term “region” as used herein refers to a physically contiguous portion of the primary structure of a biomolecule. In the case of proteins, a region is defined by a contiguous portion of the amino acid sequence of that protein. 
     The term “domain” as used herein refers to a structure of a biomolecule that contributes to a known or suspected function of the biomolecule. Domains may be co-extensive with regions or portions thereof; domains may also include distinct, non-contiguous regions of a biomolecule. Examples of protein domains include, but are not limited to, an Ig domain, an extracellular domain, a transmembrane domain, and a cytoplasmic domain. 
     As used herein, the term “molecule” means any compound, including, but not limited to, a small molecule, peptide, protein, sugar, nucleotide, nucleic acid, lipid, etc., and such a compound can be natural or synthetic. 
     “Carbon-based Products of Interest” include alcohols such as ethanol, propanol, isopropanol, butanol, fatty alcohols, fatty acid esters, wax esters; hydrocarbons and alkanes such as propane, octane, diesel, Jet Propellant 8 (JP8); polymers such as terephthalate, 1,3-propanediol, 1,4-butanediol, polyols, Polyhydroxyalkanoates (PHA), poly-beta-hydroxybutyrate (PHB), acrylate, adipic acid, ε-caprolactone, isoprene, caprolactam, rubber; commodity chemicals such as lactate, Docosahexaenoic acid (DHA), 3-hydroxypropionate, γ-valerolactone, lysine, serine, aspartate, aspartic acid, sorbitol, ascorbate, ascorbic acid, isopentenol, lanosterol, omega-3 DHA, lycopene, itaconate, 1,3-butadiene, ethylene, propylene, succinate, citrate, citric acid, glutamate, malate, 3-hydroxypropionic acid (HPA), lactic acid, THF, gamma butyrolactone, pyrrolidones, hydroxybutyrate, glutamic acid, levulinic acid, acrylic acid, malonic acid; specialty chemicals such as carotenoids, isoprenoids, itaconic acid; pharmaceuticals and pharmaceutical intermediates such as 7-aminodeacetoxycephalosporanic acid (7-ADCA)/cephalosporin, erythromycin, polyketides, statins, paclitaxel, docetaxel, terpenes, peptides, steroids, omega fatty acids and other such suitable products of interest. Such products are useful in the context of biofuels, industrial and specialty chemicals, as intermediates used to make additional products, such as nutritional supplements, neutraceuticals, polymers, paraffin replacements, personal care products and pharmaceuticals. 
     Biofuel: A biofuel refers to any fuel that derives from a biological source. Biofuel can refer to one or more hydrocarbons, one or more alcohols (such as ethanol), one or more fatty esters, or a mixture thereof. 
     Hydrocarbon: The term generally refers to a chemical compound that consists of the elements carbon (C), hydrogen (H) and optionally oxygen (O). There are essentially three types of hydrocarbons, e.g., aromatic hydrocarbons, saturated hydrocarbons and unsaturated hydrocarbons such as alkenes, alkynes, and dienes. The term also includes fuels, biofuels, plastics, waxes, solvents and oils. Hydrocarbons encompass biofuels, as well as plastics, waxes, solvents and oils. 
     Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this present invention pertains. Exemplary methods and materials are described below, although methods and materials similar or equivalent to those described herein can also be used in the practice of the present invention and will be apparent to those of skill in the art. All publications and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. The materials, methods, and examples are illustrative only and not intended to be limiting. 
     Throughout this specification and claims, the word “comprise” or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers. 
     Nucleic Acid Sequences 
     The present invention provides isolated nucleic acid molecules for genes encoding enzymes, and variants thereof. Exemplary full-length nucleic acid sequences for genes encoding enzymes and the corresponding amino acid sequences are presented in Tables 1 and 2. 
     In one embodiment, the present invention provides an isolated nucleic acid molecule having a nucleic acid sequence comprising or consisting of a gene coding for an alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, a phosphopanthetheinyl transferase, a long-chain fatty acid CoA-ligase, and/or a long-chain acyl-CoA reductase and homologs, variants and derivatives thereof expressed in a host cell of interest. The present invention also provides a nucleic acid molecule comprising or consisting of a sequence which is a codon-optimized version of the alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, a phosphopanthetheinyl transferase, a long-chain fatty acid CoA-ligase, and/or a long-chain acyl-CoA reductase genes described herein. In a further embodiment, the present invention provides a nucleic acid molecule and homologs, variants and derivatives of the molecule comprising or consisting of a sequence which is a variant of the alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, a phosphopanthetheinyl transferase, a long-chain fatty acid CoA-ligase, and/or a long-chain acyl-CoA reductase gene having at least 80% identity to the wild-type gene. The nucleic acid sequence can be preferably greater than 80%, 85%, 90%, 95%, 98%, 99%, 99.9% or even higher identity to the wild-type gene. 
     In another embodiment, the nucleic acid molecule of the present invention encodes a polypeptide having an amino acid sequence disclosed in Tables 1 and 2. Preferably, the nucleic acid molecule of the present invention encodes a polypeptide sequence of at least 50%, 60, 70%, 80%, 85%, 90% or 95% identity to the amino acid sequences shown in Tables 1 and 2 and the identity can even more preferably be 96%, 97%, 98%, 99%, 99.9% or even higher. 
     The present invention also provides nucleic acid molecules that hybridize under stringent conditions to the above-described nucleic acid molecules. As defined above, and as is well known in the art, stringent hybridizations are performed at about 25° C. below the thermal melting point (T m ) for the specific DNA hybrid under a particular set of conditions, where the T m  is the temperature at which 50% of the target sequence hybridizes to a perfectly matched probe. Stringent washing is performed at temperatures about 5° C. lower than the T m  for the specific DNA hybrid under a particular set of conditions. 
     Nucleic acid molecules comprising a fragment of any one of the above-described nucleic acid sequences are also provided. These fragments preferably contain at least 20 contiguous nucleotides. More preferably the fragments of the nucleic acid sequences contain at least 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or even more contiguous nucleotides. 
     The nucleic acid sequence fragments of the present invention display utility in a variety of systems and methods. For example, the fragments may be used as probes in various hybridization techniques. Depending on the method, the target nucleic acid sequences may be either DNA or RNA. The target nucleic acid sequences may be fractionated (e.g., by gel electrophoresis) prior to the hybridization, or the hybridization may be performed on samples in situ. One of skill in the art will appreciate that nucleic acid probes of known sequence find utility in determining chromosomal structure (e.g., by Southern blotting) and in measuring gene expression (e.g., by Northern blotting). In such experiments, the sequence fragments are preferably detectably labeled, so that their specific hydridization to target sequences can be detected and optionally quantified. One of skill in the art will appreciate that the nucleic acid fragments of the present invention may be used in a wide variety of blotting techniques not specifically described herein. 
     It should also be appreciated that the nucleic acid sequence fragments disclosed herein also find utility as probes when immobilized on microarrays. Methods for creating microarrays by deposition and fixation of nucleic acids onto support substrates are well known in the art. Reviewed in  DNA Microarrays: A Practical Approach  (Practical Approach Series), Schena (ed.), Oxford University Press (1999) (ISBN: 0199637768); Nature Genet. 21(1)(suppl):1-60 (1999);  Microarray Biochip Tools and Technology , Schena (ed.), Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN: 1881299376), the disclosures of which are incorporated herein by reference in their entireties. Analysis of, for example, gene expression using microarrays comprising nucleic acid sequence fragments, such as the nucleic acid sequence fragments disclosed herein, is a well-established utility for sequence fragments in the field of cell and molecular biology. Other uses for sequence fragments immobilized on microarrays are described in Gerhold et al.,  Trends Biochem. Sci.  24:168-173 (1999) and Zweiger,  Trends Biotechnol.  17:429-436 (1999);  DNA Microarrays: A Practical Approach  (Practical Approach Series), Schena (ed.), Oxford University Press (1999) (ISBN: 0199637768); Nature Genet. 21(1)(suppl):1-60 (1999);  Microarray Biochip: Tools and Technology , Schena (ed.), Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN: 1881299376), the disclosure of each of which is incorporated herein by reference in its entirety. 
     As is well known in the art, enzyme activities can be measured in various ways. For example, the pyrophosphorolysis of OMP may be followed spectroscopically (Grubmeyer et al., (1993)  J. Biol. Chem.  268:20299-20304). Alternatively, the activity of the enzyme can be followed using chromatographic techniques, such as by high performance liquid chromatography (Chung and Sloan, (1986)  J. Chromatogr.  371:71-81). As another alternative the activity can be indirectly measured by determining the levels of product made from the enzyme activity. These levels can be measured with techniques including aqueous chloroform/methanol extraction as known and described in the art (Cf. M. Kates (1986)  Techniques of Lipidology; Isolation, analysis and identification of Lipids . Elsevier Science Publishers, New York (ISBN: 0444807322)). More modern techniques include using gas chromatography linked to mass spectrometry (Niessen, W. M. A. (2001).  Current practice of gas chromatography—mass spectrometry . New York, N.Y.: Marcel Dekker. (ISBN: 0824704738)). Additional modern techniques for identification of recombinant protein activity and products including liquid chromatography-mass spectrometry (LCMS), high performance liquid chromatography (HPLC), capillary electrophoresis, Matrix-Assisted Laser Desorption Ionization time of flight-mass spectrometry (MALDI-TOF MS), nuclear magnetic resonance (NMR), near-infrared (NIR) spectroscopy, viscometry (Knothe, G (1997)  Am. Chem. Soc. Symp. Series,  666: 172-208), titration for determining free fatty acids (Komers (1997)  Fett/Lipid,  99(2): 52-54), enzymatic methods (Bailer (1991)  Fresenius J. Anal. Chem.  340(3): 186), physical property-based methods, wet chemical methods, etc. can be used to analyze the levels and the identity of the product produced by the organisms of the present invention. Other methods and techniques may also be suitable for the measurement of enzyme activity, as would be known by one of skill in the art. 
     Vectors 
     Also provided are vectors, including expression vectors, which comprise the above nucleic acid molecules of the present invention, as described further herein. In a first embodiment, the vectors include the isolated nucleic acid molecules described above. In an alternative embodiment, the vectors of the present invention include the above-described nucleic acid molecules operably linked to one or more expression control sequences. The vectors of the instant invention may thus be used to express a polypeptide contributing to alkane producing activity by a host cell. 
     Vectors useful for expression of nucleic acids in prokaryotes are well known in the art. 
     Isolated Polypeptides 
     According to another aspect of the present invention, isolated polypeptides (including muteins, allelic variants, fragments, derivatives, and analogs) encoded by the nucleic acid molecules of the present invention are provided. In one embodiment, the isolated polypeptide comprises the polypeptide sequence corresponding to a polypeptide sequence shown in Table 1 or 2. In an alternative embodiment of the present invention, the isolated polypeptide comprises a polypeptide sequence at least 85% identical to a polypeptide sequence shown in Table 1 or 2. Preferably the isolated polypeptide of the present invention has at least 50%, 60, 70%, 80%, 85%, 90%, 95%, 98%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9% or even higher identity to a polypeptide sequence shown in Table 1 or 2. 
     According to other embodiments of the present invention, isolated polypeptides comprising a fragment of the above-described polypeptide sequences are provided. These fragments preferably include at least 20 contiguous amino acids, more preferably at least 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or even more contiguous amino acids. 
     The polypeptides of the present invention also include fusions between the above-described polypeptide sequences and heterologous polypeptides. The heterologous sequences can, for example, include sequences designed to facilitate purification, e.g. histidine tags, and/or visualization of recombinantly-expressed proteins. Other non-limiting examples of protein fusions include those that permit display of the encoded protein on the surface of a phage or a cell, fusions to intrinsically fluorescent proteins, such as green fluorescent protein (GFP), and fusions to the IgG Fc region. 
     Host Cell Transformants 
     In another aspect of the present invention, host cells transformed with the nucleic acid molecules or vectors of the present invention, and descendants thereof, are provided. In some embodiments of the present invention, these cells carry the nucleic acid sequences of the present invention on vectors, which may but need not be freely replicating vectors. In other embodiments of the present invention, the nucleic acids have been integrated into the genome of the host cells. 
     In an alternative embodiment, the host cells of the present invention can be mutated by recombination with a disruption, deletion or mutation of the isolated nucleic acid of the present invention so that the activity of one or more enzyme(s) in the host cell is reduced or eliminated compared to a host cell lacking the mutation. 
     Selected or Engineered Microorganisms for the Production of Carbon-Based Products of Interest 
     Microorganism: Includes prokaryotic and eukaryotic microbial species from the Domains Archaea, Bacteria and Eucarya, the latter including yeast and filamentous fungi, protozoa, algae, or higher Protista. The terms “microbial cells” and “microbes” are used interchangeably with the term microorganism. 
     A variety of host organisms can be transformed to produce a product of interest. Photoautotrophic organisms include eukaryotic plants and algae, as well as prokaryotic cyanobacteria, green-sulfur bacteria, green non-sulfur bacteria, purple sulfur bacteria, and purple non-sulfur bacteria. 
     Extremophiles are also contemplated as suitable organisms. Such organisms withstand various environmental parameters such as temperature, radiation, pressure, gravity, vacuum, desiccation, salinity, pH, oxygen tension, and chemicals. They include hyperthermophiles, which grow at or above 80° C. such as  Pyrolobus fumarii ; thermophiles, which grow between 60-80° C. such as  Synechococcus lividis ; mesophiles, which grow between 15-60° C. and psychrophiles, which grow at or below 15° C. such as  Psychrobacter  and some insects. Radiation tolerant organisms include  Deinococcus radiodurans . Pressure-tolerant organisms include piezophiles, which tolerate pressure of 130 MPa. Weight-tolerant organisms include barophiles. Hypergravity (e.g., &gt;1 g) hypogravity (e.g., &lt;1 g) tolerant organisms are also contemplated. Vacuum tolerant organisms include tardigrades, insects, microbes and seeds. Dessicant tolerant and anhydrobiotic organisms include xerophiles such as  Artemia salina ; nematodes, microbes, fungi and lichens. Salt-tolerant organisms include halophiles (e.g., 2-5 M NaCl)  Halobacteriacea  and  Dunaliella salina . pH-tolerant organisms include alkaliphiles such as  Natronobacterium, Bacillus firmus  OF4,  Spirulina  spp. (e.g., pH&gt;9) and acidophiles such as  Cyanidium caldarium, Ferroplasma  sp. (e.g., low pH). Anaerobes, which cannot tolerate O 2  such as  Methanococcus jannaschii ; microaerophils, which tolerate some O 2  such as  Clostridium  and aerobes, which require O 2  are also contemplated. Gas-tolerant organisms, which tolerate pure CO 2  include  Cyanidium caldarium  and metal tolerant organisms include metalotolerants such as  Ferroplasma acidarmanus  (e.g., Cu, As, Cd, Zn),  Ralstonia  sp. CH34 (e.g., Zn, Co, Cd, Hg, Pb). Gross, Michael.  Life on the Edge: Amazing Creatures Thriving in Extreme Environments . New York: Plenum (1998) and Seckbach, J. “Search for Life in the Universe with Terrestrial Microbes Which Thrive Under Extreme Conditions.” In Cristiano Batalli Cosmovici, Stuart Bowyer, and Dan Wertheimer, eds.,  Astronomical and Biochemical Origins and the Search for Life in the Universe , p. 511. Milan: Editrice Compositori (1997). 
     Plants include but are not limited to the following genera:  Arabidopsis, Beta, Glycine, Jatropha, Miscanthus, Panicum, Phalaris, Populus, Saccharum, Salix, Simmondsia  and  Zea.    
     Algae and cyanobacteria include but are not limited to the following genera:  Acanthoceras, Acanthococcus, Acaryochloris, Achnanthes, Achnanthidium, Actinastrum, Actinochloris, Actinocyclus, Actinotaenium, Amphichrysis, Amphidinium, Amphikrikos, Amphipleura, Amphiprora, Amphithrix, Amphora, Anabaena, Anabaenopsis, Aneumastus, Ankistrodesmus, Ankyra, Anomoeoneis, Apatococcus, Aphanizomenon, Aphanocapsa, Aphanochaete, Aphanothece, Apiocystis, Apistonema, Arthrodesmus, Artherospira, Ascochloris, Asterionella, Asterococcus, Audouinella, Aulacoseira, Bacillaria, Balbiania, Bambusina, Bangia, Basichlamys, Batrachospermum, Binuclearia, Bitrichia, Blidingia, Botrdiopsis, Botrydium, Botryococcus, Botryosphaerella, Brachiomonas, Brachysira, Brachytrichia, Brebissonia, Bulbochaete, Bumilleria, Bumilleriopsis, Caloneis, Calothrix, Campylodiscus, Capsosiphon, Carteria, Catena, Cavinula, Centritractus, Centronella, Ceratium, Chaetoceros, Chaetochloris, Chaetomorpha, Chaetonella, Chaetonema, Chaetopeltis, Chaetophora, Chaetosphaeridium, Chamaesiphon, Chara, Characiochloris, Characiopsis, Characium, Charales, Chilomonas, Chlainomonas, Chlamydoblepharis, Chlamydocapsa, Chlamydomonas, Chlamydomonopsis, Chlamydomyxa, Chlamydonephris, Chlorangiella, Chlorangiopsis, Chlorella, Chlorobotrys, Chlorobrachis, Chlorochytrium, Chlorococcum, Chlorogloea, Chlorogloeopsis, Chlorogonium, Chlorolobion, Chloromonas, Chlorophysema, Chlorophyta, Chlorosaccus, Chlorosarcina, Choricystis, Chromophyton, Chromulina, Chroococcidiopsis, Chroococcus, Chroodactylon, Chroomonas, Chroothece, Chrysamoeba, Chrysapsis, Chrysidiastrum, Chrysocapsa, Chrysocapsella, Chrysochaete, Chrysochromulina, Chrysococcus, Chrysocrinus, Chrysolepidomonas, Chrysolykos, Chrysonebula, Chrysophyta, Chrysopyxis, Chrysosaccus, Chrysophaerella, Chrysostephanosphaera, Clodophora, Clastidium, Closteriopsis, Closterium, Coccomyxa, Cocconeis, Coelastrella, Coelastrum, Coelosphaerium, Coenochloris, Coenococcus, Coenocystis, Colacium, Coleochaete, Collodictyon, Compsogonopsis, Compsopogon, Conjugatophyta, Conochaete, Coronastrum, Cosmarium, Cosmioneis, Cosmocladium, Crateriportula, Craticula, Crinalium, Crucigenia, Crucigeniella, Cryptoaulax, Cryptomonas, Cryptophyta, Ctenophora, Cyanodictyon, Cyanonephron, Cyanophora, Cyanophyta, Cyanothece, Cyanothomonas, Cyclonexis, Cyclostephanos, Cyclotella, Cylindrocapsa, Cylindrocystis, Cylindrospermum, Cylindrotheca, Cymatopleura, Cymbella, Cymbellonitzschia, Cystodinium Dactylococcopsis, Debarya, Denticula, Dermatochrysis, Dermocarpa, Dermocarpella, Desmatractum, Desmidium, Desmococcus, Desmonema, Desmosiphon, Diacanthos, Diacronema, Diadesmis, Diatoma, Diatomella, Dicellula, Dichothrix, Dichotomococcus, Dicranochaete, Dictyochloris, Dictyococcus, Dictyosphaerium, Didymocystis, Didymogenes, Didymosphenia, Dilabifilum, Dimorphococcus, Dinobryon, Dinococcus, Diplochloris, Diploneis, Diplostauron, Distrionella, Docidium, Draparnaldia, Dunaliella, Dysmorphococcus, Ecballocystis, Elakatothrix, Ellerbeckia, Encyonema, Enteromorpha, Entocladia, Entomoneis, Entophysalis, Epichrysis, Epipyxis, Epithemia, Eremosphaera, Euastropsis, Euastrum, Eucapsis, Eucocconeis, Eudorina, Euglena, Euglenophyta, Eunotia, Eustigmatophyta, Eutreptia, Fallacia, Fischerella, Fragilaria, Fragilariforma, Franceia, Frustulia, Curcilla, Geminella, Genicularia, Glaucocystis, Glaucophyta, Glenodiniopsis, Glenodinium, Gloeocapsa, Gloeochaete, Gloeochrysis, Gloeococcus, Gloeocystis, Gloeodendron, Gloeomonas, Gloeoplax, Gloeothece, Gloeotila, Gloeotrichia, Gloiodictyon, Golenkinia, Golenkiniopsis, Gomontia, Gomphocymbella, Gomphonema, Gomphosphaeria, Gonatozygon, Gongrosia, Gongrosira, Goniochloris, Gonium, Gonyostomum, Granulochloris, Granulocystopsis, Groenbladia, Gymnodinium, Gymnozyga, Gyrosigma, Haematococcus, Hafniomonas, Hallassia, Hammatoidea, Hannaea, Hantzschia, Hapalosiphon, Haplotaenium, Haptophyta, Haslea, Hemidinium, Hemitoma, Heribaudiella, Heteromastix, Heterothrix, Hibberdia, Hildenbrandia, Hillea, Holopedium, Homoeothrix, Hormanthonema, Hormotila, Hyalobrachion, Hyalocardium, Hyalodiscus, Hyalogonium, Hyalotheca, Hydrianum, Hydrococcus, Hydrocoleum, Hydrocoryne, Hydrodictyon, Hydrosera, Hydrurus, Hyella, Hymenomonas, Isthmochloron, Johannesbaptistia, Juranyiella, Karayevia, Kathablepharis, Katodinium, Kephyrion, Keratococcus, Kirchneriella, Klebsormidium, Kolbesia, Koliella, Komarekia, Korshikoviella, Kraskella, Lagerheimia, Lagynion, Lamprothamnium, Lemanea, Lepocinclis, Leptosira, Lobococcus, Lobocystis, Lobomonas, Luticola, Lyngbya, Malleochloris, Mallomonas, Mantoniella, Marssoniella, Martyana, Mastigocoleus, Gastogloia, Melosira, Merismopedia, Mesostigma, Mesotaenium, Micractinium, Micrasterias, Microchaete, Microcoleus, Microcystis, Microglena, Micromonas, Microspora, Microthamnion, Mischococcus, Monochrysis, Monodus, Monomastix, Monoraphidium, Monostroma, Mougeotia, Mougeotiopsis, Myochloris, Myromecia, Myxosarcina, Naegeliella, Nannochloris, Nautococcus, Navicula, Neglectella, Neidium, Nephroclamys, Nephrocytium, Nephrodiella, Nephroselmis, Netrium, Nitella, Nitellopsis, Nitzschia, Nodularia, Nostoc, Ochromonas, Oedogonium, Oligochaetophora, Onychonema, Oocardium, Oocystis, Opephora, Ophiocytium, Orthoseira, Oscillatoria, Oxyneis, Pachycladella, Palmella, Palmodictyon, Pnadorina, Pannus, Paralia, Pascherina, Paulschulzia, Pediastrum, Pedinella, Pedinomonas, Pedinopera, Pelagodictyon, Penium, Peranema, Peridiniopsis, Peridinium, Peronia, Petroneis, Phacotus, Phacus, Phaeaster, Phaeodermatium, Phaeophyta, Phaeosphaera, Phaeothamnion, Phormidium, Phycopeltis, Phyllariochloris, Phyllocardium, Phyllomitas, Pinnularia, Pitophora, Placoneis, Planctonema, Planktosphaeria, Planothidium, Plectonema, Pleodorina, Pleurastrum, Pleurocapsa, Pleurocladia, Pleurodiscus, Pleurosigma, Pleurosira, Pleurotaenium, Pocillomonas, Podohedra, Polyblepharides, Polychaetophora, Polyedriella, Polyedriopsis, Polygoniochloris, Polyepidomonas, Polytaenia, Polytoma, Polytomella, Porphyridium, Posteriochromonas, Prasinochloris, Prasinocladus, Prasinophyta, Prasiola, Prochlorphyta, Prochlorothrix, Protoderma, Protosiphon, Provasoliella, Prymnesium, Psammodictyon, Psammothidium, Pseudanabaena, Pseudenoclonium, Psuedocarteria, Pseudochate, Pseudocharacium, Pseudococcomyxa, Pseudodictyosphaerium, Pseudokephyrion, Pseudoncobyrsa, Pseudoquadrigula, Pseudosphaerocystis, Pseudostaurastrum, Pseudostaurosira, Pseudotetrastrum, Pteromonas, Punctastruata, Pyramichlamys, Pyramimonas, Pyrrophyta, Quadrichloris, Quadricoccus, Quadrigula, Radiococcus, Radiofilum, Raphidiopsis, Raphidocelis, Raphidonema, Raphidophyta, Peimeria, Rhabdoderma, Rhabdomonas, Rhizoclonium, Rhodomonas, Rhodophyta, Rhoicosphenia, Rhopalodia, Rivularia, Rosenvingiella, Rossithidium, Roya, Scenedesmus, Scherffelia, Schizochlamydella, Schizochlamys, Schizomeris, Schizothrix, Schroederia, Scolioneis, Scotiella, Scotiellopsis, Scourfieldia, Scytonema, Selenastrum, Selenochloris, Sellaphora, Semiorbis, Siderocelis, Diderocystopsis, Dimonsenia, Siphononema, Sirocladium, Sirogonium, Skeletonema, Sorastrum, Spermatozopsis, Sphaerellocystis, Sphaerellopsis, Sphaerodinium, Sphaeroplea, Sphaerozosma, Spiniferomonas, Spirogyra, Spirotaenia, Spirulina, Spondylomorum, Spondylosium, Sporotetras, Spumella, Staurastrum, Stauerodesmus, Stauroneis, Staurosira, Staurosirella, Stenopterobia, Stephanocostis, Stephanodiscus, Stephanoporos, Stephanosphaera, Stichococcus, Stichogloea, Stigeoclonium, Stigonema, Stipitococcus, Stokesiella, Strombomonas, Stylochrysalis, Stylodinium, Styloyxis, Stylosphaeridium, Surirella, Sykidion, Symploca, Synechococcus, Synechocystis, Synedra, Synochromonas, Synura, Tabellaria, Tabularia, Teilingia, Temnogametum, Tetmemorus, Tetrachlorella, Tetracyclus, Tetradesmus, Tetraedriella, Tetraedron, Tetraselmis, Tetraspora, Tetrastrum, Thalassiosira, Thamniochaete, Thorakochloris, Thorea, Tolypella, Tolypothrix, Trachelomonas, Trachydiscus, Trebouxia, Trentepholia, Treubaria, Tribonema, Trichodesmium, Trichodiscus, Trochiscia, Tryblionella, Ulothrix, Uroglena, Uronema, Urosolenia, Urospora, Uva, Vacuolaria, Vaucheria, Volvox, Volvulina, Westella, Woloszynskia, Xanthidium, Xanthophyta, Xenococcus, Zygnema, Zygnemopsis , and  Zygonium . Cyanobacteria include members of the genus  Chamaesiphon, Chroococcus, Cyanobacterium, Cyanobium, Cyanothece, Dactylococcopsis, Gloeobacter, Gloeocapsa, Gloeothece, Microcystis, Prochlorococcus, Prochloron, Synechococcus, Synechocystis, Cyanocystis, Dermocarpella, Stanieria, Xenococcus, Chroococcidiopsis, Myxosarcina, Arthrospira, Borzia, Crinalium, Geitlerinemia, Leptolyngbya, Limnothrix, Lyngbya, Microcoleus, Oscillatoria, Planktothrix, Prochlorothrix, Pseudanabaena, Spirulina, Starria, Symploca, Trichodesmium, Tychonema, Anabaena, Anabaenopsis, Aphanizomenon, Cyanospira, Cylindrospermopsis, Cylindrospermum, Nodularia, Nostoc, Scylonema, Calothrix, Rivularia, Tolypothrix, Chlorogloeopsis, Fischerella, Geitieria, Iyengariella, Nostochopsis, Stigonema  and  Thermosynechococcus.    
     Green non-sulfur bacteria include but are not limited to the following genera:  Chloroflexus, Chloronema, Oscillochloris, Heliothrix, Herpetosiphon, Roseiflexus , and  Thermomicrobium.    
     Green sulfur bacteria include but are not limited to the following genera:  Chlorobium, Clathrochloris , and  Prosthecochloris.    
     Purple sulfur bacteria include but are not limited to the following genera:  Allochromatium, Chromatium, Halochromatium, Isochromatium, Marichromatium, Rhodovulum, Thermochromatium, Thiocapsa, Thiorhodococcus , and  Thiocystis,    
     Purple non-sulfur bacteria include but are not limited to the following genera:  Phaeospirillum, Rhodobaca, Rhodobacter, Rhodomicrobium, Rhodopila, Rhodopseudomonas, Rhodothalassium, Rhodospirillum, Rodovibrio , and  Roseospira.    
     Aerobic chemolithotrophic bacteria include but are not limited to nitrifying bacteria such as  Nitrobacteraceae  sp.,  Nitrobacter  sp.,  Nitrospina  sp.,  Nitrococcus  sp.,  Nitrospira  sp.,  Nitrosomonas  sp.,  Nitrosococcus  sp.,  Nitrosospira  sp.,  Nitrosolobus  sp.,  Nitrosovibrio  sp.; colorless sulfur bacteria such as,  Thiovulum  sp.,  Thiobacillus  sp.,  Thiomicrospira  sp.,  Thiosphaera  sp.,  Thermothrix  sp.; obligately chemolithotrophic hydrogen bacteria such as  Hydrogenobacter  sp., iron and manganese-oxidizing and/or depositing bacteria such as  Siderococcus  sp., and magnetotactic bacteria such as  Aquaspirillum  sp. 
     Archaeobacteria include but are not limited to methanogenic archaeobacteria such as  Methanobacterium  sp.,  Methanobrevibacter  sp.,  Methanothermus  sp.,  Methanococcus  sp.,  Methanomicrobium  sp.,  Methanospirillum  sp.,  Methanogenium  sp.,  Methanosarcina  sp.,  Methanolobus  sp.,  Methanothrix  sp.,  Methanococcoides  sp.,  Methanoplanus  sp.; extremely thermophilic S-Metabolizers such as  Thermoproteus  sp.,  Pyrodictium  sp.,  Sulfolobus  sp.,  Acidianus  sp. and other microorganisms such as,  Bacillus subtilis, Saccharomyces cerevisiae, Streptomyces  sp.,  Ralstonia  sp.,  Rhodococcus  sp.,  Corynebacteria  sp.,  Brevibacteria  sp.,  Mycobacteria  sp., and oleaginous yeast. 
     Preferred organisms for the manufacture of alkanes according to the methods disclosed herein include:  Arabidopsis thaliana, Panicum virgatum, Miscanthus giganteus , and  Zea mays  (plants);  Botryococcus braunii, Chlamydomonas reinhardtii  and  Dunaliela salina  (algae);  Synechococcus  sp PCC 7002,  Synechococcus  sp. PCC 7942,  Synechocystis  sp. PCC 6803 , Thermosynechococcus elongatus  BP-1 (cyanobacteria);  Chlorobium tepidum  (green sulfur bacteria),  Chloroflexus auranticus  (green non-sulfur bacteria);  Chromatium tepidum  and  Chromatium vinosum  (purple sulfur bacteria);  Rhodospirillum rubrum, Rhodobacter capsulatus , and  Rhodopseudomonas palusris  (purple non-sulfur bacteria). 
     Yet other suitable organisms include synthetic cells or cells produced by synthetic genomes as described in Venter et al. US Pat. Pub. No. 2007/0264688, and cell-like systems or synthetic cells as described in Glass et al. US Pat. Pub. No. 2007/0269862. 
     Still, other suitable organisms include microorganisms that can be engineered to fix carbon dioxide bacteria such as  Escherichia coli, Acetobacter aced, Bacillus subtilis , yeast and fungi such as  Clostridium ljungdahlii, Clostridium thermocellum, Penicillium chrysogenum, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pseudomonas fluorescens , or  Zymomonas mobilis.    
     A suitable organism for selecting or engineering is capable of autotrophic fixation of CO 2  to products. This would cover photosynthesis and methanogenesis. Acetogenesis, encompassing the three types of CO 2  fixation; Calvin cycle, acetyl-CoA pathway and reductive TCA pathway is also covered. The capability to use carbon dioxide as the sole source of cell carbon (autotrophy) is found in almost all major groups of prokaryotes. The CO 2  fixation pathways differ between groups, and there is no clear distribution pattern of the four presently-known autotrophic pathways. See, e.g., Fuchs, G. 1989 . Alternative pathways of autotrophic CO   2    fixation , p. 365-382. In H. G. Schlegel, and B. Bowien (ed.),  Autotrophic bacteria . Springer-Verlag, Berlin, Germany. The reductive pentose phosphate cycle (Calvin-Bassham-Benson cycle) represents the CO 2  fixation pathway in almost all aerobic autotrophic bacteria, for example, the cyanobacteria. 
     Alkane production via engineered cyanobacteria, e.g., a  Synechococcus  or  Thermosynechococcus  species, is preferred. Other preferred organisms include  Synechocystis, Klebsiella oxytoca, Escherichia coli  or  Saccharomyces cerevisiae . Other prokaryotic, archaea and eukaryotic host cells are also encompassed within the scope of the present invention. 
     In some aspects, alkane production via a photosynthetic organism can be carried out using the compositions, materials, and methods described in: PCT/US2009/035937 (filed Mar. 3, 2009); and PCT/US2009/055949 (filed Sep. 3, 2009); each of which is herein incorporated by reference in its entirety, for all purposes. 
     Carbon-Based Products of Interest: Hydrocarbons &amp; Alcohols 
     In various embodiments of the invention, desired hydrocarbons and/or alcohols of certain chain length or a mixture thereof can be produced. In certain aspects, the host cell produces at least one of the following carbon-based products of interest: medium chain-length alkanes such as heptane, nonane, and/or undecane. In other aspects, the carbon chain length ranges from C 2  to C 20 . Accordingly, the invention provides production of various chain lengths of alkanes suitable for use as fuels &amp; chemicals. 
     In preferred aspects, the methods provide culturing host cells for direct product secretion for easy recovery without the need to extract biomass. These carbon-based products of interest are secreted directly into the medium. Since the invention enables production of various defined chain length of hydrocarbons and alcohols, the secreted products are easily recovered or separated. The products of the invention, therefore, can be used directly or used with minimal processing. 
     Fuel Compositions 
     In various embodiments, compositions produced by the methods of the invention are used as fuels. Such fuels comply with ASTM standards, for instance, standard specifications for diesel fuel oils D 975-09b, and Jet A, Jet A-1 and Jet B as specified in ASTM Specification D. 1655-68. Fuel compositions may require blending of several products to produce a uniform product. The blending process is relatively straightforward, but the determination of the amount of each component to include in a blend is much more difficult. Fuel compositions may, therefore, include aromatic and/or branched hydrocarbons, for instance, 75% saturated and 25% aromatic, wherein some of the saturated hydrocarbons are branched and some are cyclic. Preferably, the methods of the invention produce an array of hydrocarbons, such as C 2 -C 17  or C 10 -C 15  to alter cloud point. Furthermore, the compositions may comprise fuel additives, which are used to enhance the performance of a fuel or engine. For example, fuel additives can be used to alter the freezing/gelling point, cloud point, lubricity, viscosity, oxidative stability, ignition quality, octane level, and flash point. Fuels compositions may also comprise, among others, antioxidants, static dissipater, corrosion inhibitor, icing inhibitor, biocide, metal deactivator and thermal stability improver. 
     In addition to many environmental advantages of the invention such as CO 2  conversion and renewable source, other advantages of the fuel compositions disclosed herein include low sulfur content, low emissions, being free or substantially free of alcohol and having high cetane number. 
     Example 1 
     Crude Extract of  E. coli  Cells Overexpressing acrM Convert Lauroyl-CoA to Dodecanal and Decanoyl-CoA to Decanal 
       Acinetobacter  sp. M-1 acyl coenzyme A reductase, acrM, was codon-optimized for  E. coli  expression and synthesized by DNA2.0 (Menlo Park, CA; SEQ ID NO. 1) with a NdeI site on the 5′ end and an EcoRI site on the 3′ end. The obtained gene was subcloned into a pET28a vector (Novagen) by digestion with NdeI and EcoRI and subsequent ligation. The resulting plasmid, pET28a-acrM (SEQ ID NO. 2), containing an N-terminal His 6 -tagged acrM (“His 6 ” disclosed as SEQ ID NO: 18), was transformed into a BL 21 (DE 3 )  E. coli  strain, which was subsequently grown with shaking in Luria-Bertani medium supplemented with 100 μg/mL of kanomycin in a volume of 1 L to OD 600  =0.8 before induction with 0.25 mM IPTG for 5 hours in a 2-L shaker flask at 37° C. An SDS-PAGE gel demonstrating the overexpression of AcrM protein in pET28a-acrM containing BL21(DE3)  E. coli  cells is shown in  FIG. 1 . 
     The  E. coli  cells containing overexpressed AcrM were collected by centrifugation, resuspended in HEPES buffer (100 mM HEPES, 10% glycerol, pH 7.5) at a 1:3 (w/v) ratio and lysed by sonication. 200 μL of buffer solution containing 100 μL total lysate, 1 mM acyl-CoA, 3 mM NADH (Sigma-Aldrich), 100 mM HEPES, 10% glycerol at pH 7.5 was incubated at 37° C. for 30 min, extracted with 100 μL ethyl acetate and analyzed by GC/MS equipped with a HP-5 ms column (Agilent, Santa Clara, Calif.). Total ion chromatography (TIC) indicated the detection of aldehydes produced from corresponding acyl-CoA substrates by the AcrM-containing cell extract in the presence of supplemented NADH, as shown in  FIG. 2 . 
     Example 2 
     Feeding Fatty Acid to  Svnechococcus  Sp. PCC 7002 Strain Expressing adm-carB-entD Results in Detection of Corresponding Aldehyde and Alkane 
     The carboxylic acid reductase (carB) gene (SEQ ID NO. 3) was PCR-amplified from  Mycobacterium smegmatis  and verified by sequencing with multiple primers by Genewiz (South Plainfield, N.J.). Cyanothece adm,  E. coli  leaderless tesA and  E. coli  entD genes were codon-optimized for  E. coli  overexpression and synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NO. 4 and 5) with an individual ribosome binding site in front of each gene. All four genes were subcloned into a pUC 19 vector containing an ammonia-repressible P(nir07) promoter, upstream/downstream homology regions, and a spectinomycin marker. The resulting plasmid, pAQ3::P(nir07)-adm-carB-tesA-entD-SpecR (SEQ ID NO. 6), was transformed into wild-type  Synechococcus  sp. PCC 7002 and segregated in the presence of spectinomycin. 
     The expression and activity of the Adm, CarB, TesA, and EntD proteins were demonstrated by detection of tridecane and pentadecane in the transformed  Synechococcus  sp. PCC 7002 strain by GC/FID ( FIG. 3 ). 
     The  Synechococcus  sp. PCC 7002 cultures were grown to OD 730 ˜5 before 1 mM fatty acid (100 mM stock in ethanol) was added and were then shaken at 150 rpm, 37° C. for ˜3 hours in the absence (lauric acid feeding) or presence (octanoic acid and decanoic acid feeding) of a pentadecane overlay (6 mL culture with 1 mL overlay). The pentadecane overlay from the octanoic acid-fed culture ( FIGS. 4A and 4D ), or decanoic acid culture ( FIGS. 4B and 4E ) was analyzed by GC/MS equipped with an HP-5 ms column. For the lauric acid feeding assay, 1 mL culture was extracted with 400 μL hexane by vortexing for 1 min before being analyzed by GC/MS ( FIG. 4C ,  4 F). Note that the pAQ3::P(nir07)-adm-carB-tesA-entD-SpecR expressing  Synechococcus  sp. PCC 7002 strain can produce a detectable level of undecane even without feeding dodecanoic acid. 
     Example 3 
       Synechococcus  Sp. PCC 7002 Strain Expressing adm-carB-fatB2-entD Results in Increased Detection of Nonane in Pentadecane Overlay 
     The  E. coli  leaderless tesA of pAQ3::P(nir07)-adm-carB-tesA-entD-SpecR, was replaced by  Cuphea hookeriana  leaderless fatB2 (a medium-chain acyl-ACP thioesterase), which was codon-optimized for  E. coli  overexpression and synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NO. 7), with an individual ribosome binding site in front of the gene, a 5′ Kpn I restriction site and a 3′ Hind III restriction site. The resulting plasmid, pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR (SEQ ID NO. 8), was transformed into wild-type  Synechococcus  sp. PCC 7002 and segregated in the presence of spectinomycin. 
     The wild type  Synechococcus  sp. PCC 7002 and pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR expressing  Synechococcus  sp. PCC 7002 cultures (35 mL) were grown to OD 730 ˜3 (in the presence of 2 mM urea) before a 10 mL pentadecane overlay was added. The cultures were shaken at 150 rpm, 37° C. for 3 more days continuously. 100 μL pentadecane overlay samples from each flask were taken 12 hours ( FIG. 5A ) or 72 hours ( FIG. 5B ) after pentadecane addition, respectively, and analyzed directly by GC/FID equipped with a 20 meter hp-5 ms column. An increase of nonane production was detected in the pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR expressing  Synechococcus  sp. PCC 7002 cultures but not in the wild type control ones. A relative increase in octane and heptanes production was also detected in the pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR expressing  Synechococcus  sp. PCC 7002 cultures (data not shown). 
     Example 4 
     Medium Chain-Length Alkane Production 
     One or more recombinant genes encoding one or more enzymes having enzyme activities which catalyze the production of medium chain-length alkanes are identified and selected. The enzyme activities include: an alkane deformylative monooxygenase activity, a thioesterase activity, a carboxylic acid reductase activity, and a phosphopanthetheinyl transferase activity, a long-chain fatty acid CoA-ligase activity, and/or a long-chain acyl-CoA reductase activity. Such genes and enzymes can be those described in Tables 1 and 2. 
     The selected genes are cloned into an expression vector. For example, adm-carB-entD-fatB or adm-acrM-fadD-fatB (or combinations of homologs thereof) are cloned into one or more vectors. See  FIG. 6 . The genes can be under inducible control (such as the urea-repressible nir07 promoter or the cumate-inducible cum02 promoter). The genes may or may not be expressed operonically; and one or more of the genes can be placed under constitutive control such that when the other gene(s) are induced, the genes under constitutive control are already expressed. For example, one might express adm, carB, and entD constitutively while placing fatty-acid-generating fatB under inducible control; thus when fatty acids are made by fatB after induction, the remainder of the pathway is already present. 
     One or more vectors are selected and transformed into a microorganism (e.g., cyanobacteria). The cells are grown to a suitable optical density. In some instances cells are grown to a suitable optical density in an uninduced state, and then an induction signal is applied to commence alkane production. 
     Alkanes are produced by the transformed cells. The alkanes generally have 7, 8, 9, 10, or 11 carbon atoms. In some instances, alkanes are detected. In some instances, alkanes are quantified. In some instances, alkanes are collected. 
     In some aspects, a thioesterase such as fatB can be used. To test downstream of fatB, fatty acids of various chain lengths are fed along with inorganic carbon (e.g., CO 2 ) to cells, and alkane production is monitored. After fatB addition, cells are provided with inorganic carbon (e.g., CO 2 ) and alkane production is monitored. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 SEQ 
                   
                   
               
               
                 ID 
                   
                   
               
               
                 NO 
                 DESCRIPTION 
                 SEQUENCE 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 1 
                 acrM (from 
                 ATGAATGCAAAACTGAAGAAATTGTTCCAGCAGAAAGTAGACGGCAAGACCATCAT 
               
               
                   
                 
                   Acinetobacter 
                 
                 CGTGACCGGTGCAAGCAGCGGTATTGGCTTGACCGTGAGCAAATACCTGGCTCAGG 
               
               
                   
                 sp. M-1), 
                 CGGGTGCACACGTGCTGCTGCTGGCGCGTACGAAAGAGAAACTGGATGAGGTCAAG 
               
               
                   
                 codon- 
                 GCGGAGATTGAAGCGGAAGGCGGTAAGGCTACTGTTTTCCCGTGCGATTTGAATGA 
               
               
                   
                 optimized for 
                 CATGGAATCCATTGACGCAGTCAGCAAAGAGATCCTGGCAGCCGTTGATCATATCG 
               
               
                   
                   E .  coli   
                 ACATTCTGGTGAATAACGCGGGTCGCAGCATCCGTCGCGCGGTCCACGAAAGCGTG 
               
               
                   
                   
                 GATCGCTTCCATGACTTTGAGCGTACCATGCAACTGAATTACTTCGGTGCCGTTCG 
               
               
                   
                   
                 TCTGGTCCTGAATGTTCTGCCGCACATGATGCAGCGCAAAGATGGCCAAATCATTA 
               
               
                   
                   
                 ACATTAGCAGCATTGGCGTTTTGGCGAACGCGACGCGTTTCAGCGCGTATGTGGCG 
               
               
                   
                   
                 AGCAAGGCTGCACTGGATGCCTTCTCCCGTTGTCTGAGCGCCGAGGTCCATTCGCA 
               
               
                   
                   
                 CAAGATTGCGATTACCTCTATCTATATGCCGCTGGTTCGTACCCCGATGATTGCGC 
               
               
                   
                   
                 CGACGAAGATCTACAAGTATGTCCCAACGTTGTCCCCGGAAGAGGCGGCTGACCTG 
               
               
                   
                   
                 ATTGCTTATGCGATCGTTAAACGTCCGAAAAAGATCGCCACCAATCTGGGTCGCCT 
               
               
                   
                   
                 GGCAAGCATCACCTACGCGATTGCCCCGGACATCAACAACATCCTGATGAGCATCG 
               
               
                   
                   
                 GCTTTAACCTGTTTCCGTCTAGCACGGCGAGCGTGGGTGAGCAAGAAAAGCTGAAC 
               
               
                   
                   
                 CTGATTCAACGTGCCTACGCACGTCTGTTTCCTGGTGAACACTGGTAA 
               
               
                   
               
               
                 2 
                 Plasmid 
                 TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTA 
               
               
                   
                 pET28a-acrM 
                 CGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTC 
               
               
                   
                   
                 TTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGG 
               
               
                   
                   
                 GCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTG 
               
               
                   
                   
                 ATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCT 
               
               
                   
                   
                 TTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAAC 
               
               
                   
                   
                 ACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGG 
               
               
                   
                   
                 CCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAA 
               
               
                   
                   
                 ATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCC 
               
               
                   
                   
                 TATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCT 
               
               
                   
                   
                 TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATC 
               
               
                   
                   
                 AATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGC 
               
               
                   
                   
                 AGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACA 
               
               
                   
                   
                 TCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATC 
               
               
                   
                   
                 ACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCC 
               
               
                   
                   
                 AGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACC 
               
               
                   
                   
                 AAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTT 
               
               
                   
                   
                 AAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCG 
               
               
                   
                   
                 CATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTT 
               
               
                   
                   
                 TTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATG 
               
               
                   
                   
                 CTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCAT 
               
               
                   
                   
                 CTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCA 
               
               
                   
                   
                 TCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCG 
               
               
                   
                   
                 AGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAG 
               
               
                   
                   
                 AGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG 
               
               
                   
                   
                 TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCC 
               
               
                   
                   
                 ACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTT 
               
               
                   
                   
                 CTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTG 
               
               
                   
                   
                 TTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAG 
               
               
                   
                   
                 CGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAG 
               
               
                   
                   
                 AACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGC 
               
               
                   
                   
                 TGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGG 
               
               
                   
                   
                 ATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG 
               
               
                   
                   
                 CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCAC 
               
               
                   
                   
                 GCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG 
               
               
                   
                   
                 GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTC 
               
               
                   
                   
                 GGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCG 
               
               
                   
                   
                 GAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCT 
               
               
                   
                   
                 GGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGT 
               
               
                   
                   
                 ATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAG 
               
               
                   
                   
                 CGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGC 
               
               
                   
                   
                 ATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTG 
               
               
                   
                   
                 ATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGG 
               
               
                   
                   
                 CTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTC 
               
               
                   
                   
                 CCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAG 
               
               
                   
                   
                 GTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGT 
               
               
                   
                   
                 GGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGT 
               
               
                   
                   
                 TTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGT 
               
               
                   
                   
                 TTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGG 
               
               
                   
                   
                 TAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA 
               
               
                   
                   
                 CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCG 
               
               
                   
                   
                 GGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAG 
               
               
                   
                   
                 GTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTG 
               
               
                   
                   
                 CAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCAT 
               
               
                   
                   
                 TCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCT 
               
               
                   
                   
                 CGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGG 
               
               
                   
                   
                 GTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGAT 
               
               
                   
                   
                 AATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAG 
               
               
                   
                   
                 CGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTC 
               
               
                   
                   
                 CAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTAC 
               
               
                   
                   
                 GAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCG 
               
               
                   
                   
                 CCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCC 
               
               
                   
                   
                 GGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTT 
               
               
                   
                   
                 CCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGA 
               
               
                   
                   
                 GAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGG 
               
               
                   
                   
                 CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA 
               
               
                   
                   
                 CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATA 
               
               
                   
                   
                 TAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAAC 
               
               
                   
                   
                 GCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGG 
               
               
                   
                   
                 CAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGA 
               
               
                   
                   
                 AAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATT 
               
               
                   
                   
                 GCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTA 
               
               
                   
                   
                 ATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACG 
               
               
                   
                   
                 CCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTC 
               
               
                   
                   
                 AGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG 
               
               
                   
                   
                 CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGA 
               
               
                   
                   
                 AGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACAC 
               
               
                   
                   
                 CACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCG 
               
               
                   
                   
                 ACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTG 
               
               
                   
                   
                 CCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGC 
               
               
                   
                   
                 TTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG 
               
               
                   
                   
                 AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGT 
               
               
                   
                   
                 TTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCG 
               
               
                   
                   
                 AAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGAC 
               
               
                   
                   
                 TCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGC 
               
               
                   
                   
                 AAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTG 
               
               
                   
                   
                 CCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCT 
               
               
                   
                   
                 TCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGT 
               
               
                   
                   
                 GATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATT 
               
               
                   
                   
                 AATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAA 
               
               
                   
                   
                 TTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCA 
               
               
                   
                   
                 TCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCAT ATGAATGCAAAACTGAAGAAAT   
               
               
                   
                   
                 
                   TGTTCCAGCAGAAAGTAGACGGCAAGACCATCATCGTGACCGGTGCAAGCAGCGGT 
                 
               
               
                   
                   
                 
                   ATTGGCTTGACCGTGAGCAAATACCTGGCTCAGGCGGGTGCACACGTGCTGCTGCT 
                 
               
               
                   
                   
                 
                   GGCGCGTACGAAAGAGAAACTGGATGAGGTCAAGGCGGAGATTGAAGCGGAAGGCG 
                 
               
               
                   
                   
                 
                   GTAAGGCTACTGTTTTCCCGTGCGATTTGAATGACATGGAATCCATTGACGCAGTC 
                 
               
               
                   
                   
                 
                   AGCAAAGAGATCCTGGCAGCCGTTGATCATATCGACATTCTGGTGAATAACGCGGG 
                 
               
               
                   
                   
                 
                   TCGCAGCATCCGTCGCGCGGTCCACGAAAGCGTGGATCGCTTCCATGACTTTGAGC 
                 
               
               
                   
                   
                 
                   GTACCATGCAACTGAATTACTTCGGTGCCGTTCGTCTGGTCCTGAATGTTCTGCCG 
                 
               
               
                   
                   
                 
                   CACATGATGCAGCGCAAAGATGGCCAAATCATTAACATTAGCAGCATTGGCGTTTT 
                 
               
               
                   
                   
                 
                   GGCGAACGCGACGCGTTTCAGCGCGTATGTGGCGAGCAAGGCTGCACTGGATGCCT 
                 
               
               
                   
                   
                 
                   TCTCCCGTTGTCTGAGCGCCGAGGTCCATTCGCACAAGATTGCGATTACCTCTATC 
                 
               
               
                   
                   
                 
                   TATATGCCGCTGGTTCGTACCCCGATGATTGCGCCGACGAAGATCTACAAGTATGT 
                 
               
               
                   
                   
                 
                   CCCAACGTTGTCCCCGGAAGAGGCGGCTGACCTGATTGCTTATGCGATCGTTAAAC 
                 
               
               
                   
                   
                 
                   GTCCGAAAAAGATCGCCACCAATCTGGGTCGCCTGGCAAGCATCACCTACGCGATT 
                 
               
               
                   
                   
                 
                   GCCCCGGACATCAACAACATCCTGATGAGCATCGGCTTTAACCTGTTTCCGTCTAG 
                 
               
               
                   
                   
                 
                   CACGGCGAGCGTGGGTGAGCAAGAAAAGCTGAACCTGATTCAACGTGCCTACGCAC 
                 
               
               
                   
                   
                   GTCTGTTTCCTGGTGAACACTGGTAA GAATTCGAGCTCCGTCGACAAGCTTGCGGC 
               
               
                   
                   
                 CGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAA 
               
               
                   
                   
                 AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGG 
               
               
                   
                   
                 GCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT 
               
               
                   
               
               
                 3 
                 carboxylic 
                   GAGCTCGAGGAGGTTTTTACA ATGACCAGCGATGTTCACGACGCCACAGACGGCGT 
               
               
                   
                 acid 
                 CACCGAAACCGCACTCGACGACGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACG 
               
               
                   
                 reductase 
                 CCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGACGCGGCG 
               
               
                   
                 amplified 
                 CACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACGG 
               
               
                   
                 from 
                 TGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGC 
               
               
                   
                 
                   Mycobacterium 
                 
                 GCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGG 
               
               
                   
                   smegmatis . 
                 TCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTA 
               
               
                   
                   
                 CCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTGACGCTGG 
               
               
                   
                   
                 ATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACCG 
               
               
                   
                   
                 GTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAG 
               
               
                   
                   
                 CGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGC 
               
               
                   
                   
                 AGCTCGTGGTGTTCGACCATCACCCCGAGGTCGACGACCACCGCGACGCACTGGCC 
               
               
                   
                   
                 CGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGGACGCGAT 
               
               
                   
                   
                 CGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGATC 
               
               
                   
                   
                 AGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCG 
               
               
                   
                   
                 ATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGA 
               
               
                   
                   
                 CCCCACGCCGGTCATCAACGTCAACTTCATGCCGCTCAACCACCTGGGCGGGCGCA 
               
               
                   
                   
                 TCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACCGGAATCC 
               
               
                   
                   
                 GACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCCT 
               
               
                   
                   
                 GGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCC 
               
               
                   
                   
                 TGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTG 
               
               
                   
                   
                 CGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGGATTCGTCAGCACCGCACCGCT 
               
               
                   
                   
                 GGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATCGTCGACG 
               
               
                   
                   
                 GCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCCA 
               
               
                   
                   
                 CCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGA 
               
               
                   
                   
                 CAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGT 
               
               
                   
                   
                 ACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTACTACCAC 
               
               
                   
                   
                 ACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGGACCGTCG 
               
               
                   
                   
                 CAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAGG 
               
               
                   
                   
                 CGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAG 
               
               
                   
                   
                 CGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGA 
               
               
                   
                   
                 TCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCACGCGACG 
               
               
                   
                   
                 CCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGAGCCGTTC 
               
               
                   
                   
                 AGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCAA 
               
               
                   
                   
                 AGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGG 
               
               
                   
                   
                 CCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACC 
               
               
                   
                   
                 CTCACCCAGGCCGCTGCCACGATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGC 
               
               
                   
                   
                 CCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCGAACCTGC 
               
               
                   
                   
                 TGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCACC 
               
               
                   
                   
                 AACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAG 
               
               
                   
                   
                 GCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGC 
               
               
                   
                   
                 TGACCCTGGACAAGTTCATCGACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCC 
               
               
                   
                   
                 AAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCTGGCTGGG 
               
               
                   
                   
                 CCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTCA 
               
               
                   
                   
                 TCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCC 
               
               
                   
                   
                 TACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCT 
               
               
                   
                   
                 GCGGGTGGTCGCCGGTGACATCGGCGACCCGAATCTGGGCCTCACACCCGAGATCT 
               
               
                   
                   
                 GGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCTGGTCAAC 
               
               
                   
                   
                 CACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGGT 
               
               
                   
                   
                 GATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGT 
               
               
                   
                   
                 CGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGC 
               
               
                   
                   
                 CCGGTGCGCCCGCTCGACGGCGGATACGCCAACGGCTACGGCAACAGCAAGTGGGC 
               
               
                   
                   
                 CGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTGGCGACGT 
               
               
                   
                   
                 TCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCCA 
               
               
                   
                   
                 GACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTC 
               
               
                   
                   
                 GTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCG 
               
               
                   
                   
                 ATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTG 
               
               
                   
                   
                 TCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGTTCGTGGA 
               
               
                   
                   
                 CTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGGG 
               
               
                   
                   
                 TGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACC 
               
               
                   
                   
                 GTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACC 
               
               
                   
                   
                 CGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAG 
               
               
                   
                   
                 ACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCTGCGTGAG 
               
               
                   
                   
                 TTCGGTCTGATCTGA GGTACC   
               
               
                   
               
               
                 4 
                 codon- 
                   CAT ATGCAAGAACTGGCCCTGAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAA 
               
               
                   
                 optimized 
                 AGATGCGTATAGCCGTATTAACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACC 
               
               
                   
                 
                   Cyanothece 
                 
                 AAAACTACCTGGACATGGCGCAACTGCTGCCGGAGGACGAGGCTGAGCTGATTCGT 
               
               
                   
                 adm. 
                 TTGAGCAAGATGGAGAACCGTCACAAAAAGGGTTTTCAAGCGTGCGGCAAGAACCT 
               
               
                   
                   
                 CAATGTGACTCCGGATATGGATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCA 
               
               
                   
                   
                 ATTTTCAGAAGGCTAAAGCCGAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGC 
               
               
                   
                   
                 CTGATCATCGAGGCGTTTGCGATTGCAGCCTACAACATTTACATTCCAGTGGCTGA 
               
               
                   
                   
                 TCCGTTTGCACGTAAAATCACCGAGGGTGTCGTCAAGGATGAGTATACCCACCTGA 
               
               
                   
                   
                 ATTTCGGCGAAGTTTGGTTGAAGGAACATTTTGAAGCAAGCAAGGCGGAGTTGGAG 
               
               
                   
                   
                 GACGCCAACAAAGAGAACTTACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAA 
               
               
                   
                   
                 GGATGCCGAAGTGCTGGGTATGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTA 
               
               
                   
                   
                 GCTATGGTGAGGCACTGAGCAACATCGGCTTTTCTACGAGAGAAATCATGAAGATG 
               
               
                   
                   
                 AGCGCGTACGGTCTGCGTGCAGCATAA GAGCTC   
               
               
                   
               
               
                 5 
                 codon- 
                   GAGCTCGAGGAGGTTTTTACA ATGACCAGCGATGTTCACGACGCCACAGACGGCGT 
               
               
                   
                 optimized  E . 
                 CACCGAAACCGCACTCGACGACGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACG 
               
               
                   
                   coli  tesA and 
                 CCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGACGCGGCG 
               
               
                   
                   E .  coli  entD 
                 CACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACGG 
               
               
                   
                 genes. 
                 TGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGC 
               
               
                   
                   
                 GCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGG 
               
               
                   
                   
                 TCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTA 
               
               
                   
                   
                 CCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTGACGCTGG 
               
               
                   
                   
                 ATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACCG 
               
               
                   
                   
                 GTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAG 
               
               
                   
                   
                 CGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGC 
               
               
                   
                   
                 AGCTCGTGGTGTTCGACCATCACCCCGAGGTCGACGACCACCGCGACGCACTGGCC 
               
               
                   
                   
                 CGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGGACGCGAT 
               
               
                   
                   
                 CGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGATC 
               
               
                   
                   
                 AGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCG 
               
               
                   
                   
                 ATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGA 
               
               
                   
                   
                 CCCCACGCCGGTCATCAACGTCAACTTCATGCCGCTCAACCACCTGGGCGGGCGCA 
               
               
                   
                   
                 TCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACCGGAATCC 
               
               
                   
                   
                 GACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCCT 
               
               
                   
                   
                 GGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCC 
               
               
                   
                   
                 TGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTG 
               
               
                   
                   
                 CGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGGATTCGTCAGCACCGCACCGCT 
               
               
                   
                   
                 GGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATCGTCGACG 
               
               
                   
                   
                 GCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCCA 
               
               
                   
                   
                 CCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGA 
               
               
                   
                   
                 CAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGT 
               
               
                   
                   
                 ACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTACTACCAC 
               
               
                   
                   
                 ACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGGACCGTCG 
               
               
                   
                   
                 CAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAGG 
               
               
                   
                   
                 CGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAG 
               
               
                   
                   
                 CGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGA 
               
               
                   
                   
                 TCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCACGCGACG 
               
               
                   
                   
                 CCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGAGCCGTTC 
               
               
                   
                   
                 AGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCAA 
               
               
                   
                   
                 AGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGG 
               
               
                   
                   
                 CCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACC 
               
               
                   
                   
                 CTCACCCAGGCCGCTGCCACGATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGC 
               
               
                   
                   
                 CCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCGAACCTGC 
               
               
                   
                   
                 TGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCACC 
               
               
                   
                   
                 AACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAG 
               
               
                   
                   
                 GCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGC 
               
               
                   
                   
                 TGACCCTGGACAAGTTCATCGACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCC 
               
               
                   
                   
                 AAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCTGGCTGGG 
               
               
                   
                   
                 CCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTCA 
               
               
                   
                   
                 TCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCC 
               
               
                   
                   
                 TACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCT 
               
               
                   
                   
                 GCGGGTGGTCGCCGGTGACATCGGCGACCCGAATCTGGGCCTCACACCCGAGATCT 
               
               
                   
                   
                 GGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCTGGTCAAC 
               
               
                   
                   
                 CACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGGT 
               
               
                   
                   
                 GATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGT 
               
               
                   
                   
                 CGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGC 
               
               
                   
                   
                 CCGGTGCGCCCGCTCGACGGCGGATACGCCAACGGCTACGGCAACAGCAAGTGGGC 
               
               
                   
                   
                 CGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTGGCGACGT 
               
               
                   
                   
                 TCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCCA 
               
               
                   
                   
                 GACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTC 
               
               
                   
                   
                 GTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCG 
               
               
                   
                   
                 ATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTG 
               
               
                   
                   
                 TCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGTTCGTGGA 
               
               
                   
                   
                 CTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGGG 
               
               
                   
                   
                 TGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACC 
               
               
                   
                   
                 GTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACC 
               
               
                   
                   
                 CGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAG 
               
               
                   
                   
                 ACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCTGCGTGAG 
               
               
                   
                   
                 TTCGGTCTGATCTGA GGTACCAGGAGGTTTTTACA ATGGCTGATACTTTGTTGATT 
               
               
                   
                   
                 TTGGGTGATTCTCTCTCTGCAGGCTACCGTATGTCCGCGAGCGCGGCATGGCCGGC 
               
               
                   
                   
                 TCTGCTGAACGATAAGTGGCAGAGCAAGACCAGCGTGGTCAATGCGAGCATCAGCG 
               
               
                   
                   
                 GCGATACCAGCCAGCAGGGTCTGGCACGTCTGCCAGCGCTGCTGAAGCAACACCAG 
               
               
                   
                   
                 CCGCGTTGGGTGCTGGTTGAACTGGGCGGCAATGACGGTCTGCGTGGTTTTCAGCC 
               
               
                   
                   
                 GCAGCAGACCGAACAAACGTTGCGTCAGATTCTGCAGGACGTCAAGGCGGCTAACG 
               
               
                   
                   
                 CGGAACCGCTGCTGATGCAAATTCGCCTGCCGGCGAATTATGGTCGTCGTTACAAC 
               
               
                   
                   
                 GAGGCTTTCAGCGCCATTTATCCTAAACTGGCTAAAGAGTTTGACGTGCCGCTGCT 
               
               
                   
                   
                 GCCGTTCTTCATGGAAGAGGTCTACCTGAAACCGCAATGGATGCAAGACGACGGTA 
               
               
                   
                   
                 TTCATCCGAATCGTGATGCACAACCTTTCATCGCGGATTGGATGGCGAAGCAATTG 
               
               
                   
                   
                 CAACCGCTGGTGAACCATGACTCGTAAAAGCTTGTTGCTGCATGCAGGAGGTTTTT 
               
               
                   
                   
                 ACAATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGT 
               
               
                   
                   
                 CGAATTTGATCCGGCGAACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATG 
               
               
                   
                   
                 CCCAGCTGCAGCACGCAGGCCGTAAGCGTAAAACTGAACATCTGGCCGGTCGCATT 
               
               
                   
                   
                 GCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCGGCCATTGGTGA 
               
               
                   
                   
                 ACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTA 
               
               
                   
                   
                 CTACCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATA 
               
               
                   
                   
                 TTCTCTGTCCAGACGGCACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCA 
               
               
                   
                   
                 CGAGCGTCTGGCGGACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCCTGGCATTCA 
               
               
                   
                   
                 GCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATGCGGGCTTCCTG 
               
               
                   
                   
                 GATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGA 
               
               
                   
                   
                 GATGTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGC 
               
               
                   
                   
                 ACGACTGA GAATTC   
               
               
                   
               
               
                 6 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCC 
               
               
                   
                 pAQ3::Pnir07_ 
                 CTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGA 
               
               
                   
                 adm_carB_tesA_ 
                 TCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACA 
               
               
                   
                 entD_SpecR. 
                 AAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT 
               
               
                   
                   
                 TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTC 
               
               
                   
                   
                 GCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAC 
               
               
                   
                   
                 CGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGG 
               
               
                   
                   
                 GGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAC 
               
               
                   
                   
                 CTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGG 
               
               
                   
                   
                 GAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGT 
               
               
                   
                   
                 CGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGC 
               
               
                   
                   
                 GGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTG 
               
               
                   
                   
                 CGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGG 
               
               
                   
                   
                 CGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCC 
               
               
                   
                   
                 TTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCT 
               
               
                   
                   
                 CGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAA 
               
               
                   
                   
                 AACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATA 
               
               
                   
                   
                 AATGAGAAAAAAGCAACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAA 
               
               
                   
                   
                 CACCTATAATTAAACTATTCATCTATTATTTATGATTTTTTGTATATACAATATTT 
               
               
                   
                   
                 CTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATAATAAAGGGAAAATC 
               
               
                   
                   
                 AGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCG 
               
               
                   
                   
                 CTGAACCTGCAGGCGAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAA 
               
               
                   
                   
                 CCCGTCGCCATTGACCCAGAACCGCGCAAAGAACGGGAAAAAATTGATCTCGATCT 
               
               
                   
                   
                 GGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAAAGTGAAGTTAGCCG 
               
               
                   
                   
                 ATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGA 
               
               
                   
                   
                 CCTCTTCAAGGATGAAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTA 
               
               
                   
                   
                 AATCGTTCCTGACCGGACTCGCGGAAAAAGGCTACGGTGACACCCAACTGAAGGCG 
               
               
                   
                   
                 ATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGATGTCCTGACTTGGGT 
               
               
                   
                   
                 TGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGAC 
               
               
                   
                   
                 CAATACATTCGAGAAGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCAT 
               
               
                   
                   
                 CGAAATCAAATACCAAACCGTTAATGAAGGTTTAGTGATCTTGGGTCAGGATATCG 
               
               
                   
                   
                 GTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAGATGTGGCATAAAAA 
               
               
                   
                   
                 AGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCA 
               
               
                   
                   
                 AAAAGTATCAATGATTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGC 
               
               
                   
                   
                 ATGATTTACAAAAAGTTGTAGTTTCTGTTACCAATTGCGAATCGAGAACTGCCTAA 
               
               
                   
                   
                 TCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATGCAAGAACTGGCCCT 
               
               
                   
                   
                 GAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAAAGATGCGTATAGCCGTATTA 
               
               
                   
                   
                 ACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACCAAAACTACCTGGACATGGCG 
               
               
                   
                   
                 CAACTGCTGCCGGAGGACGAGGCTGAGCTGATTCGTTTGAGCAAGATGGAGAACCG 
               
               
                   
                   
                 TCACAAAAAGGGTTTTCAAGCGTGCGGCAAGAACCTCAATGTGACTCCGGATATGG 
               
               
                   
                   
                 ATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCAATTTTCAGAAGGCTAAAGCC 
               
               
                   
                   
                 GAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGCCTGATCATCGAGGCGTTTGC 
               
               
                   
                   
                 GATTGCAGCCTACAACATTTACATTCCAGTGGCTGATCCGTTTGCACGTAAAATCA 
               
               
                   
                   
                 CCGAGGGTGTCGTCAAGGATGAGTATACCCACCTGAATTTCGGCGAAGTTTGGTTG 
               
               
                   
                   
                 AAGGAACATTTTGAAGCAAGCAAGGCGGAGTTGGAGGACGCCAACAAAGAGAACTT 
               
               
                   
                   
                 ACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAAGGATGCCGAAGTGCTGGGTA 
               
               
                   
                   
                 TGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTAGCTATGGTGAGGCACTGAGC 
               
               
                   
                   
                 AACATCGGCTTTTCTACGAGAGAAATCATGAAGATGAGCGCGTACGGTCTGCGTGC 
               
               
                   
                   
                 AGCATAAGAGCTCGAGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAG 
               
               
                   
                   
                 ACGGCGTCACCGAAACCGCACTCGACGACGAGCAGTCGACCCGCCGCATCGCCGAG 
               
               
                   
                   
                 CTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGA 
               
               
                   
                   
                 CGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCG 
               
               
                   
                   
                 GCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAG 
               
               
                   
                   
                 GGCGGGCGCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCA 
               
               
                   
                   
                 GGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGC 
               
               
                   
                   
                 CGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTG 
               
               
                   
                   
                 ACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAA 
               
               
                   
                   
                 CGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCA 
               
               
                   
                   
                 CCGTGAGCGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCG 
               
               
                   
                   
                 GTGTCGCAGCTCGTGGTGTTCGACCATCACCCCGAGGTCGACGACCACCGCGACGC 
               
               
                   
                   
                 ACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGG 
               
               
                   
                   
                 ACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGAC 
               
               
                   
                   
                 CATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAA 
               
               
                   
                   
                 GGGTGCGATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCA 
               
               
                   
                   
                 CGGGTGACCCCACGCCGGTCATCAACGTCAACTTCATGCCGCTCAACCACCTGGGC 
               
               
                   
                   
                 GGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACC 
               
               
                   
                   
                 GGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAAC 
               
               
                   
                   
                 TCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTC 
               
               
                   
                   
                 GACCGCCTGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGC 
               
               
                   
                   
                 CGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGGATTCGTCAGCACCG 
               
               
                   
                   
                 CACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATC 
               
               
                   
                   
                 GTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGT 
               
               
                   
                   
                 GCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCA 
               
               
                   
                   
                 GCACCGACAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACT 
               
               
                   
                   
                 CCCGGGTACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTA 
               
               
                   
                   
                 CTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGG 
               
               
                   
                   
                 ACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAAC 
               
               
                   
                   
                 CTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAA 
               
               
                   
                   
                 CAGCGAGCGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGC 
               
               
                   
                   
                 AGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCA 
               
               
                   
                   
                 CGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGA 
               
               
                   
                   
                 GCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCA 
               
               
                   
                   
                 ACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCC 
               
               
                   
                   
                 ACGCAGGCCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGAT 
               
               
                   
                   
                 CGACACCCTCACCCAGGCCGCTGCCACGATCCTCGGCACCGGGAGCGAGGTGGCAT 
               
               
                   
                   
                 CCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCG 
               
               
                   
                   
                 AACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCC 
               
               
                   
                   
                 GGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTG 
               
               
                   
                   
                 ACCGCAGGCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCG 
               
               
                   
                   
                 AGTGAGCTGACCCTGGACAAGTTCATCGACGCCGAAACGCTCCGGGCCGCACCGGG 
               
               
                   
                   
                 TCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCT 
               
               
                   
                   
                 GGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGC 
               
               
                   
                   
                 ACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGAC 
               
               
                   
                   
                 CCAGGCCTACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACC 
               
               
                   
                   
                 GCCACCTGCGGGTGGTCGCCGGTGACATCGGCGACCCGAATCTGGGCCTCACACCC 
               
               
                   
                   
                 GAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCT 
               
               
                   
                   
                 GGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGG 
               
               
                   
                   
                 CCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCC 
               
               
                   
                   
                 ACCGTGTCGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGAC 
               
               
                   
                   
                 CGTGAGCCCGGTGCGCCCGCTCGACGGCGGATACGCCAACGGCTACGGCAACAGCA 
               
               
                   
                   
                 AGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTG 
               
               
                   
                   
                 GCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAA 
               
               
                   
                   
                 CGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGC 
               
               
                   
                   
                 CGCGGTCGTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTG 
               
               
                   
                   
                 ACGGTCGATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGG 
               
               
                   
                   
                 ATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGT 
               
               
                   
                   
                 TCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGAC 
               
               
                   
                   
                 GACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGC 
               
               
                   
                   
                 ACAGACCGTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCG 
               
               
                   
                   
                 GCGCACCCGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGC 
               
               
                   
                   
                 CCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCT 
               
               
                   
                   
                 GCGTGAGTTCGGTCTGATCTGAGGTACCAGGAGGTTTTTACAATGGCTGATACTTT 
               
               
                   
                   
                 GTTGATTTTGGGTGATTCTCTCTCTGCAGGCTACCGTATGTCCGCGAGCGCGGCAT 
               
               
                   
                   
                 GGCCGGCTCTGCTGAACGATAAGTGGCAGAGCAAGACCAGCGTGGTCAATGCGAGC 
               
               
                   
                   
                 ATCAGCGGCGATACCAGCCAGCAGGGTCTGGCACGTCTGCCAGCGCTGCTGAAGCA 
               
               
                   
                   
                 ACACCAGCCGCGTTGGGTGCTGGTTGAACTGGGCGGCAATGACGGTCTGCGTGGTT 
               
               
                   
                   
                 TTCAGCCGCAGCAGACCGAACAAACGTTGCGTCAGATTCTGCAGGACGTCAAGGCG 
               
               
                   
                   
                 GCTAACGCGGAACCGCTGCTGATGCAAATTCGCCTGCCGGCGAATTATGGTCGTCG 
               
               
                   
                   
                 TTACAACGAGGCTTTCAGCGCCATTTATCCTAAACTGGCTAAAGAGTTTGACGTGC 
               
               
                   
                   
                 CGCTGCTGCCGTTCTTCATGGAAGAGGTCTACCTGAAACCGCAATGGATGCAAGAC 
               
               
                   
                   
                 GACGGTATTCATCCGAATCGTGATGCACAACCTTTCATCGCGGATTGGATGGCGAA 
               
               
                   
                   
                 GCAATTGCAACCGCTGGTGAACCATGACTCGTAAAAGCTTGTTGCTGCATGCAGGA 
               
               
                   
                   
                 GGTTTTTACAATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTAC 
               
               
                   
                   
                 ATTTCGTCGAATTTGATCCGGCGAACTTTTGTGAACAAGACCTGTTGTGGCTGCCG 
               
               
                   
                   
                 CATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAAAACTGAACATCTGGCCGG 
               
               
                   
                   
                 TCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCGGCCA 
               
               
                   
                   
                 TTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCAC 
               
               
                   
                   
                 TGCGGTACTACCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGA 
               
               
                   
                   
                 AGAGATATTCTCTGTCCAGACGGCACGCGAGCTGACGGACAACATCATTACCCCGG 
               
               
                   
                   
                 CAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCCTG 
               
               
                   
                   
                 GCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATGCGGG 
               
               
                   
                   
                 CTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTG 
               
               
                   
                   
                 AGAATGAGATGTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTG 
               
               
                   
                   
                 TGCCAGCACGACTGAGAATTCGGTTTTCCGTCCTGTCTTGATTTTCAAGCAAACAA 
               
               
                   
                   
                 TGCCTCCGATTTCTAATCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAAAAT 
               
               
                   
                   
                 ATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCATTT 
               
               
                   
                   
                 ACTAGTTTTTAATTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGT 
               
               
                   
                   
                 GGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGG 
               
               
                   
                   
                 CATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGC 
               
               
                   
                   
                 AACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGA 
               
               
                   
                   
                 AGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGC 
               
               
                   
                   
                 GCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGC 
               
               
                   
                   
                 GGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGA 
               
               
                   
                   
                 TGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTG 
               
               
                   
                   
                 GAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATC 
               
               
                   
                   
                 ATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAA 
               
               
                   
                   
                 TGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCT 
               
               
                   
                   
                 TGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAA 
               
               
                   
                   
                 CTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAAC 
               
               
                   
                   
                 GCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGT 
               
               
                   
                   
                 TGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCT 
               
               
                   
                   
                 GCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGC 
               
               
                   
                   
                 TAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGT 
               
               
                   
                   
                 TGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAA 
               
               
                   
                   
                 TGTCTAACAATTCGTTCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAAGCGT 
               
               
                   
                   
                 TAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTT 
               
               
                   
                   
                 TATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAG 
               
               
                   
                   
                 GCGCGCCACGAGAAAGAGTTATGACAAATTAAAATTCTGACTCTTAGATTATTTCC 
               
               
                   
                   
                 AGAGAGGCTGATTTTCCCAATCTTTGGGAAAGCCTAAGTTTTTAGATTCTATTTCT 
               
               
                   
                   
                 GGATACATCTCAAAAGTTCTTTTTAAATGCTGTGCAAAATTATGCTCTGGTTTAAT 
               
               
                   
                   
                 TCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAATTTTACGGCTGTTTC 
               
               
                   
                   
                 TTTGATTAATATCCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCA 
               
               
                   
                   
                 GGCAATTTAGGTGATTCTCCTAGCTGTATATTCCAGAGCCTTGAATGATGAGCGCA 
               
               
                   
                   
                 AATATTTCTAATATGCGACAAAGACCGTAACCAAGATATAAAAAACTTGTTAGGTA 
               
               
                   
                   
                 ATTGGAAATGAGTATGTATTTTTTGTCGTGTCTTAGATGGTAATAAATTTGTGTAC 
               
               
                   
                   
                 ATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATATATGACGGCGGTAG 
               
               
                   
                   
                 TAGAGGATTTGTGTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAG 
               
               
                   
                   
                 ATTGGCAATATTGAGTAATCGAATCGATTAATTCTTGATGCTTCCCAGTGTCATAA 
               
               
                   
                   
                 AATAAACTTTTATTCAGATACCAATGAGGATCATAATCATGGGAGTAGTGATAAAT 
               
               
                   
                   
                 CATTTGAGTTCTGACTGCTACTTCTATCGACTCCGTAGCATTAAAAATAAGCATTC 
               
               
                   
                   
                 TCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCAAAAGGGCGACACCC 
               
               
                   
                   
                 CATAATTAGCCCGGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTG 
               
               
                   
                   
                 ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTA 
               
               
                   
                   
                 CGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCC 
               
               
                   
                   
                 GCCAGGCAAACAAGGGGTGTTATGAGCCATATTCAGGTATAAATGGGCTCGCGATA 
               
               
                   
                   
                 ATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATTTGTTTATTTTTCTA 
               
               
                   
                   
                 AATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAAT 
               
               
                   
                   
                 AATATTGAAAAAGGAAGAATATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC 
               
               
                   
                   
                 TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGT 
               
               
                   
                   
                 AAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCA 
               
               
                   
                   
                 ACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC 
               
               
                   
                   
                 ACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGA 
               
               
                   
                   
                 GCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAG 
               
               
                   
                   
                 TCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC 
               
               
                   
                   
                 ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACC 
               
               
                   
                   
                 GAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATC 
               
               
                   
                   
                 GTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG 
               
               
                   
                   
                 CCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCT 
               
               
                   
                   
                 AGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCAC 
               
               
                   
                   
                 TTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGT 
               
               
                   
                   
                 GAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCG 
               
               
                   
                   
                 TATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGAC 
               
               
                   
                   
                 AGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT 
               
               
                   
               
               
                 7 
                 codon- 
                   GGTACCAGGAGGTTTTTAC ATGGACCGTAAAAGCAAGCGTCCGGACATGCTGGTTG 
               
               
                   
                 optimized 
                 ATTCCTTTGGTCTGGAAAGCACCGTGCAGGACGGTCTGGTTTTCCGTCAGTCTTTC 
               
               
                   
                 
                   Cuphea 
                 
                 TCCATTCGTAGCTATGAGATTGGTACTGATCGTACCGCCTCTATCGAAACCCTGAT 
               
               
                   
                 
                   hookeriana 
                 
                 GAATCACCTGCAAGAAACCTCTCTGAACCATTGTAAGTCTACTGGCATCCTGCTGG 
               
               
                   
                 leaderless 
                 ACGGTTTCGGTCGTACCCTGGAGATGTGCAAACGCGACCTGATTTGGGTAGTGATC 
               
               
                   
                 fatB2 gene. 
                 AAAATGCAGATCAAAGTTAACCGTTATCCGGCATGGGGTGATACCGTTGAAATCAA 
               
               
                   
                   
                 CACCCGCTTTTCTCGTCTGGGCAAAATCGGTATGGGCCGTGACTGGCTGATCTCTG 
               
               
                   
                   
                 ACTGTAACACTGGTGAAATTCTGGTTCGTGCTACTAGCGCATACGCGATGATGAAC 
               
               
                   
                   
                 CAGAAAACCCGTCGCCTGAGCAAGCTGCCGTACGAGGTCCACCAGGAGATTGTTCC 
               
               
                   
                   
                 GCTGTTTGTAGACAGCCCAGTGATTGAGGATTCTGACCTGAAAGTGCATAAATTCA 
               
               
                   
                   
                 AAGTGAAGACCGGTGACAGCATCCAAAAAGGCCTGACCCCAGGTTGGAACGATCTG 
               
               
                   
                   
                 GACGTTAACCAGCACGTTTCCAACGTGAAGTATATCGGTTGGATTCTGGAGAGCAT 
               
               
                   
                   
                 GCCGACCGAGGTCCTGGAAACCCAGGAGCTGTGTTCCCTGGCGCTGGAGTACCGCC 
               
               
                   
                   
                 GTGAGTGCGGCCGTGACAGCGTGCTGGAGTCTGTGACCGCTATGGACCCAAGCAAA 
               
               
                   
                   
                 GTTGGTGTTCGTAGCCAGTACCAGCACCTGCTGCGTCTGGAAGACGGTACTGCTAT 
               
               
                   
                   
                 CGTGAACGGTGCAACTGAATGGCGTCCTAAAAACGCGGGTGCAAACGGTGCTATCA 
               
               
                   
                   
                 GCACCGGTAAAACCTCTAACGGTAACTCCGTGAGCTAA AAGCTT   
               
               
                   
               
               
                 8 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCC 
               
               
                   
                 pAQ3::P(nir07)_ 
                 CTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGA 
               
               
                   
                 adm_carB_fatB2_ 
                 TCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACA 
               
               
                   
                 entD_SpecR. 
                 AAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT 
               
               
                   
                   
                 TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTC 
               
               
                   
                   
                 GCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAC 
               
               
                   
                   
                 CGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGG 
               
               
                   
                   
                 GGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAC 
               
               
                   
                   
                 CTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGG 
               
               
                   
                   
                 GAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGT 
               
               
                   
                   
                 CGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGC 
               
               
                   
                   
                 GGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTG 
               
               
                   
                   
                 CGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGG 
               
               
                   
                   
                 CGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCC 
               
               
                   
                   
                 TTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCT 
               
               
                   
                   
                 CGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAA 
               
               
                   
                   
                 AACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATA 
               
               
                   
                   
                 AATGAGAAAAAAGCAACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAA 
               
               
                   
                   
                 CACCTATAATTAAACTATTCATCTATTATTTATGATTTTTTGTATATACAATATTT 
               
               
                   
                   
                 CTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATAATAAAGGGAAAATC 
               
               
                   
                   
                 AGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCG 
               
               
                   
                   
                 CTGAACCTGCAGGCGAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAA 
               
               
                   
                   
                 CCCGTCGCCATTGACCCAGAACCGCGCAAAGAACGGGAAAAAATTGATCTCGATCT 
               
               
                   
                   
                 GGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAAAGTGAAGTTAGCCG 
               
               
                   
                   
                 ATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGA 
               
               
                   
                   
                 CCTCTTCAAGGATGAAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTA 
               
               
                   
                   
                 AATCGTTCCTGACCGGACTCGCGGAAAAAGGCTACGGTGACACCCAACTGAAGGCG 
               
               
                   
                   
                 ATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGATGTCCTGACTTGGGT 
               
               
                   
                   
                 TGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGAC 
               
               
                   
                   
                 CAATACATTCGAGAAGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCAT 
               
               
                   
                   
                 CGAAATCAAATACCAAACCGTTAATGAAGGTTTAGTGATCTTGGGTCAGGATATCG 
               
               
                   
                   
                 GTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAGATGTGGCATAAAAA 
               
               
                   
                   
                 AGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCA 
               
               
                   
                   
                 AAAAGTATCAATGATTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGC 
               
               
                   
                   
                 ATGATTTACAAAAAGTTGTAGTTTCTGTTACCAATTGCGAATCGAGAACTGCCTAA 
               
               
                   
                   
                 TCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATGCAAGAACTGGCCCT 
               
               
                   
                   
                 GAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAAAGATGCGTATAGCCGTATTA 
               
               
                   
                   
                 ACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACCAAAACTACCTGGACATGGCG 
               
               
                   
                   
                 CAACTGCTGCCGGAGGACGAGGCTGAGCTGATTCGTTTGAGCAAGATGGAGAACCG 
               
               
                   
                   
                 TCACAAAAAGGGTTTTCAAGCGTGCGGCAAGAACCTCAATGTGACTCCGGATATGG 
               
               
                   
                   
                 ATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCAATTTTCAGAAGGCTAAAGCC 
               
               
                   
                   
                 GAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGCCTGATCATCGAGGCGTTTGC 
               
               
                   
                   
                 GATTGCAGCCTACAACATTTACATTCCAGTGGCTGATCCGTTTGCACGTAAAATCA 
               
               
                   
                   
                 CCGAGGGTGTCGTCAAGGATGAGTATACCCACCTGAATTTCGGCGAAGTTTGGTTG 
               
               
                   
                   
                 AAGGAACATTTTGAAGCAAGCAAGGCGGAGTTGGAGGACGCCAACAAAGAGAACTT 
               
               
                   
                   
                 ACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAAGGATGCCGAAGTGCTGGGTA 
               
               
                   
                   
                 TGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTAGCTATGGTGAGGCACTGAGC 
               
               
                   
                   
                 AACATCGGCTTTTCTACGAGAGAAATCATGAAGATGAGCGCGTACGGTCTGCGTGC 
               
               
                   
                   
                 AGCATAAGAGCTCGAGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAG 
               
               
                   
                   
                 ACGGCGTCACCGAAACCGCACTCGACGACGAGCAGTCGACCCGCCGCATCGCCGAG 
               
               
                   
                   
                 CTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGA 
               
               
                   
                   
                 CGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCG 
               
               
                   
                   
                 GCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAG 
               
               
                   
                   
                 GGCGGGCGCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCA 
               
               
                   
                   
                 GGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGC 
               
               
                   
                   
                 CGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTG 
               
               
                   
                   
                 ACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAA 
               
               
                   
                   
                 CGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCA 
               
               
                   
                   
                 CCGTGAGCGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCG 
               
               
                   
                   
                 GTGTCGCAGCTCGTGGTGTTCGACCATCACCCCGAGGTCGACGACCACCGCGACGC 
               
               
                   
                   
                 ACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGG 
               
               
                   
                   
                 ACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGAC 
               
               
                   
                   
                 CATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAA 
               
               
                   
                   
                 GGGTGCGATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCA 
               
               
                   
                   
                 CGGGTGACCCCACGCCGGTCATCAACGTCAACTTCATGCCGCTCAACCACCTGGGC 
               
               
                   
                   
                 GGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACC 
               
               
                   
                   
                 GGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAAC 
               
               
                   
                   
                 TCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTC 
               
               
                   
                   
                 GACCGCCTGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGC 
               
               
                   
                   
                 CGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGGATTCGTCAGCACCG 
               
               
                   
                   
                 CACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATC 
               
               
                   
                   
                 GTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGT 
               
               
                   
                   
                 GCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCA 
               
               
                   
                   
                 GCACCGACAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACT 
               
               
                   
                   
                 CCCGGGTACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTA 
               
               
                   
                   
                 CTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGG 
               
               
                   
                   
                 ACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAAC 
               
               
                   
                   
                 CTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAA 
               
               
                   
                   
                 CAGCGAGCGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGC 
               
               
                   
                   
                 AGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCA 
               
               
                   
                   
                 CGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGA 
               
               
                   
                   
                 GCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCA 
               
               
                   
                   
                 ACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCC 
               
               
                   
                   
                 ACGCAGGCCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGAT 
               
               
                   
                   
                 CGACACCCTCACCCAGGCCGCTGCCACGATCCTCGGCACCGGGAGCGAGGTGGCAT 
               
               
                   
                   
                 CCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCG 
               
               
                   
                   
                 AACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCC 
               
               
                   
                   
                 GGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTG 
               
               
                   
                   
                 ACCGCAGGCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCG 
               
               
                   
                   
                 AGTGAGCTGACCCTGGACAAGTTCATCGACGCCGAAACGCTCCGGGCCGCACCGGG 
               
               
                   
                   
                 TCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCT 
               
               
                   
                   
                 GGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGC 
               
               
                   
                   
                 ACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGAC 
               
               
                   
                   
                 CCAGGCCTACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACC 
               
               
                   
                   
                 GCCACCTGCGGGTGGTCGCCGGTGACATCGGCGACCCGAATCTGGGCCTCACACCC 
               
               
                   
                   
                 GAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCT 
               
               
                   
                   
                 GGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGG 
               
               
                   
                   
                 CCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCC 
               
               
                   
                   
                 ACCGTGTCGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGAC 
               
               
                   
                   
                 CGTGAGCCCGGTGCGCCCGCTCGACGGCGGATACGCCAACGGCTACGGCAACAGCA 
               
               
                   
                   
                 AGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTG 
               
               
                   
                   
                 GCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAA 
               
               
                   
                   
                 CGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGC 
               
               
                   
                   
                 CGCGGTCGTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTG 
               
               
                   
                   
                 ACGGTCGATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGG 
               
               
                   
                   
                 ATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGT 
               
               
                   
                   
                 TCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGAC 
               
               
                   
                   
                 GACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGC 
               
               
                   
                   
                 ACAGACCGTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCG 
               
               
                   
                   
                 GCGCACCCGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGC 
               
               
                   
                   
                 CCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCT 
               
               
                   
                   
                 GCGTGAGTTCGGTCTGATCTGAGGTACCAGGAGGTTTTTACATGGACCGTAAAAGC 
               
               
                   
                   
                 AAGCGTCCGGACATGCTGGTTGATTCCTTTGGTCTGGAAAGCACCGTGCAGGACGG 
               
               
                   
                   
                 TCTGGTTTTCCGTCAGTCTTTCTCCATTCGTAGCTATGAGATTGGTACTGATCGTA 
               
               
                   
                   
                 CCGCCTCTATCGAAACCCTGATGAATCACCTGCAAGAAACCTCTCTGAACCATTGT 
               
               
                   
                   
                 AAGTCTACTGGCATCCTGCTGGACGGTTTCGGTCGTACCCTGGAGATGTGCAAACG 
               
               
                   
                   
                 CGACCTGATTTGGGTAGTGATCAAAATGCAGATCAAAGTTAACCGTTATCCGGCAT 
               
               
                   
                   
                 GGGGTGATACCGTTGAAATCAACACCCGCTTTTCTCGTCTGGGCAAAATCGGTATG 
               
               
                   
                   
                 GGCCGTGACTGGCTGATCTCTGACTGTAACACTGGTGAAATTCTGGTTCGTGCTAC 
               
               
                   
                   
                 TAGCGCATACGCGATGATGAACCAGAAAACCCGTCGCCTGAGCAAGCTGCCGTACG 
               
               
                   
                   
                 AGGTCCACCAGGAGATTGTTCCGCTGTTTGTAGACAGCCCAGTGATTGAGGATTCT 
               
               
                   
                   
                 GACCTGAAAGTGCATAAATTCAAAGTGAAGACCGGTGACAGCATCCAAAAAGGCCT 
               
               
                   
                   
                 GACCCCAGGTTGGAACGATCTGGACGTTAACCAGCACGTTTCCAACGTGAAGTATA 
               
               
                   
                   
                 TCGGTTGGATTCTGGAGAGCATGCCGACCGAGGTCCTGGAAACCCAGGAGCTGTGT 
               
               
                   
                   
                 TCCCTGGCGCTGGAGTACCGCCGTGAGTGCGGCCGTGACAGCGTGCTGGAGTCTGT 
               
               
                   
                   
                 GACCGCTATGGACCCAAGCAAAGTTGGTGTTCGTAGCCAGTACCAGCACCTGCTGC 
               
               
                   
                   
                 GTCTGGAAGACGGTACTGCTATCGTGAACGGTGCAACTGAATGGCGTCCTAAAAAC 
               
               
                   
                   
                 GCGGGTGCAAACGGTGCTATCAGCACCGGTAAAACCTCTAACGGTAACTCCGTGAG 
               
               
                   
                   
                 CTAAAAGCTTGTTGCTGCATGCAGGAGGTTTTTACAATGAAAACGACCCACACCAG 
               
               
                   
                   
                 CTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGAACTTTT 
               
               
                   
                   
                 GTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGT 
               
               
                   
                   
                 AAGCGTAAAACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGA 
               
               
                   
                   
                 GTACGGCTACAAATGCGTGCCGGCCATTGGTGAACTGCGTCAACCGGTTTGGCCGG 
               
               
                   
                   
                 CAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTACCGCGTTGGCGGTTGTGTCT 
               
               
                   
                   
                 CGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGCACGCGA 
               
               
                   
                   
                 GCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTC 
               
               
                   
                   
                 TGGCGTTCAGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAG 
               
               
                   
                   
                 GCTTCCGAGATCCAAACCGATGCGGGCTTCCTGGATTATCAAATCATCAGCTGGAA 
               
               
                   
                   
                 CAAGCAACAGGTTATCATTCACCGTGAGAATGAGATGTTTGCCGTCCATTGGCAGA 
               
               
                   
                   
                 TTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGAGAATTCGGTTTTCCG 
               
               
                   
                   
                 TCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGT 
               
               
                   
                   
                 TTTTGTTTATTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGC 
               
               
                   
                   
                 CTACCGTCATAAATAATTTGCCATTTACTAGTTTTTAATTAACCAGAACCTTGACC 
               
               
                   
                   
                 GAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTT 
               
               
                   
                   
                 TTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGG 
               
               
                   
                   
                 GTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCT 
               
               
                   
                   
                 AAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACT 
               
               
                   
                   
                 ATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTAC 
               
               
                   
                   
                 ATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTG 
               
               
                   
                   
                 CTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGA 
               
               
                   
                   
                 CCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAG 
               
               
                   
                   
                 TCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAA 
               
               
                   
                   
                 CTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGC 
               
               
                   
                   
                 CACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTG 
               
               
                   
                   
                 CCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTA 
               
               
                   
                   
                 TTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGG 
               
               
                   
                   
                 CGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCG 
               
               
                   
                   
                 GCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCC 
               
               
                   
                   
                 CAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGA 
               
               
                   
                   
                 TCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCG 
               
               
                   
                   
                 AGATCACCAAGGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCCGC 
               
               
                   
                   
                 TTCGCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAG 
               
               
                   
                   
                 CCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCT 
               
               
                   
                   
                 ACACAAATTGGGAGATATATCATGAGGCGCGCCACGAGAAAGAGTTATGACAAATT 
               
               
                   
                   
                 AAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTTTCCCAATCTTTGGGAA 
               
               
                   
                   
                 AGCCTAAGTTTTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAATGC 
               
               
                   
                   
                 TGTGCAAAATTATGCTCTGGTTTAATTCTGTCTAAGAGATACTGAATACAACATAA 
               
               
                   
                   
                 GCCAGTGAAAATTTTACGGCTGTTTCTTTGATTAATATCCTCCAATACTTCTCTAG 
               
               
                   
                   
                 AGAGCCATTTTCCTTTTAACCTATCAGGCAATTTAGGTGATTCTCCTAGCTGTATA 
               
               
                   
                   
                 TTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATATGCGACAAAGACCGTAA 
               
               
                   
                   
                 CCAAGATATAAAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCGTG 
               
               
                   
                   
                 TCTTAGATGGTAATAAATTTGTGTACATTCTAGATAACTGCCCAAAGGCGATTATC 
               
               
                   
                   
                 TCCAAAGCCATATATGACGGCGGTAGTAGAGGATTTGTGTACTTGTTTCGATAATG 
               
               
                   
                   
                 CCCGATAAATTCTTCTACTTTTTTAGATTGGCAATATTGAGTAATCGAATCGATTA 
               
               
                   
                   
                 ATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATTCAGATACCAATGAGGA 
               
               
                   
                   
                 TCATAATCATGGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATCGA 
               
               
                   
                   
                 CTCCGTAGCATTAAAAATAAGCATTCTCAAGGATTTATCAAACTTGTATAGATTTG 
               
               
                   
                   
                 GCCGGCCCGTCAAAAGGGCGACACCCCATAATTAGCCCGGGCGAAAGGCCCAGTCT 
               
               
                   
                   
                 TTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGG 
               
               
                   
                   
                 GAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG 
               
               
                   
                   
                 TCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCCAT 
               
               
                   
                   
                 ATTCAGGTATAAATGGGCTCGCGATAATGTTCAGAATTGGTTAATTGGTTGTAACA 
               
               
                   
                   
                 CTGACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAG 
               
               
                   
                   
                 ACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAATATGAGTATTC 
               
               
                   
                   
                 AACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT 
               
               
                   
                   
                 GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACG 
               
               
                   
                   
                 AGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCC 
               
               
                   
                   
                 CCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTA 
               
               
                   
                   
                 TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCA 
               
               
                   
                   
                 GAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGA 
               
               
                   
                   
                 CAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC 
               
               
                   
                   
                 TTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACAT 
               
               
                   
                   
                 GGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC 
               
               
                   
                   
                 CAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAA 
               
               
                   
                   
                 CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGAT 
               
               
                   
                   
                 GGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGT 
               
               
                   
                   
                 TTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCG 
               
               
                   
                   
                 CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCA 
               
               
                   
                   
                 GGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA 
               
               
                   
                   
                 AGCATTGGT 
               
               
                   
               
               
                 9 
                 carB 
                 MTSDVHDATDGVTETALDDEQSTRRIAELYATDPEFAAAAPLPAVVDAAHKPGLRL 
               
               
                   
                 
                   Mycobacterium 
                 
                 AEILQTLFTGYGDRPALGYRARELATDEGGRTVTRLLPRFDTLTYAQVWSRVQAVA 
               
               
                   
                 
                   smegmatis 
                 
                 AALRHNFAQPIYPGDAVATIGFASPDYLTLDLVCAYLGLVSVPLQHNAPVSRLAPI 
               
               
                   
                   
                 LAEVEPRILTVSAEYLDLAVESVRDVNSVSQLVVFDHHPEVDDHRDALARAREQLA 
               
               
                   
                   
                 GKGIAVTTLDAIADEGAGLPAEPIYTADHDQRLAMILYTSGSTGAPKGAMYTEAMV 
               
               
                   
                   
                 ARLWTMSFITGDPTPVINVNFMPLNHLGGRIPISTAVQNGGTSYFVPESDMSTLFE 
               
               
                   
                   
                 DLALVRPTELGLVPRVADMLYQHHLATVDRLVTQGADELTAEKQAGAELREQVLGG 
               
               
                   
                   
                 RVITGFVSTAPLAAEMRAFLDITLGAHIVDGYGLTETGAVTRDGVIVRPPVIDYKL 
               
               
                   
                   
                 IDVPELGYFSTDKPYPRGELLVRSQTLTPGYYKRPEVTASVFDRDGYYHTGDVMAE 
               
               
                   
                   
                 TAPDHLVYVDRRNNVLKLAQGEFVAVANLEAVFSGAALVRQIFVYGNSERSFLLAV 
               
               
                   
                   
                 VVPTPEALEQYDPAALKAALADSLQRTARDAELQSYEVPADFIVETEPFSAANGLL 
               
               
                   
                   
                 SGVGKLLRPNLKDRYGQRLEQMYADIAATQANQLRELRRAAATQPVIDTLTQAAAT 
               
               
                   
                   
                 ILGTGSEVASDAHFTDLGGDSLSALTLSNLLSDFFGFEVPVGTIVNPATNLAQLAQ 
               
               
                   
                   
                 HIEAQRTAGDRRPSFTTVHGADATEIRASELTLDKFIDAETLRAAPGLPKVTTEPR 
               
               
                   
                   
                 TVLLSGANGWLGRFLTLQWLERLAPVGGTLITIVRGRDDAAARARLTQAYDTDPEL 
               
               
                   
                   
                 SRRFAELADRHLRVVAGDIGDPNLGLTPEIWHRLAAEVDLVVHPAALVNHVLPYRQ 
               
               
                   
                   
                 LFGPNVVGTAEVIKLALTERIKPVTYLSTVSVAMGIPDFEEDGDIRTVSPVRPLDG 
               
               
                   
                   
                 GYANGYGNSKWAGEVLLREAHDLCGLPVATFRSDMILAHPRYRGQVNVPDMFTRLL 
               
               
                   
                   
                 LSLLITGVAPRSFYIGDGERPRAHYPGLTVDFVAEAVTTLGAQQREGYVSYDVMNP 
               
               
                   
                   
                 HDDGISLDVFVDWLIRAGHPIDRVDDYDDWVRRFETALTALPEKRRAQTVLPLLHA 
               
               
                   
                   
                 FRAPQAPLRGAPEPTEVFHAAVRTAKVGPGDIPHLDEALIDKYIRDLREFGLI 
               
               
                   
               
               
                 10 
                 entD  E . coli   
                 MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIA 
               
               
                   
                   
                 AVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEEIF 
               
               
                   
                   
                 SVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEIQTDAGFLD 
               
               
                   
                   
                 YQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD 
               
               
                   
               
               
                 11 
                 acrM 
                 MNAKLKKLFQQKVDGKTIIVTGASSGIGLTVSKYLAQAGAHVLLLARTKEKLDEVK 
               
               
                   
                 
                   Acinetobacter 
                 
                 AEIEAEGGKATVFPCDLNDMESIDAVSKEILAAVDHIDILVNNAGRSIRRAVHESV 
               
               
                   
                 sp. M-1 
                 DRFHDFERTMQLNYFGAVRLVLNVLPHMMQRKDGQIINISSIGVLANATRFSAYVA 
               
               
                   
                   
                 SKAALDAFSRCLSAEVHSHKIAITSIYMPLVRTPMIAPTKIYKYVPTLSPEEAADL 
               
               
                   
                   
                 IAYAIVKRPKKIATNLGRLASITYAIAPDINNILMSIGFNLFPSSTASVGEQEKLN 
               
               
                   
                   
                 LIQRAYARLFPGEHW 
               
               
                   
               
               
                 12 
                 fadD  E . coli   
                 MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVNMGEVMTFRKLEE 
               
               
                   
                   
                 RSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRE 
               
               
                   
                   
                 LEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAKGTVVNFVV 
               
               
                   
                   
                 KYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTTGVAKGA 
               
               
                   
                   
                 MLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELGGQNL 
               
               
                   
                   
                 LITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMP 
               
               
                   
                   
                 VQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV 
               
               
                   
                   
                 DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFL 
               
               
                   
                   
                 RIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKK 
               
               
                   
                   
                 DPSLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNK 
               
               
                   
                   
                 A 
               
               
                   
               
               
                 13 
                 fatB(C12 
                 MATTSLASAFCSMKAVMLARDGRGMKPRSSDLQLRAGNAPTSLKMINGTKFSYTES 
               
               
                   
                 fatty acid) 
                 LKRLPDWSMLFAVITTIFSAAEKQWTNLEWKPKPKLPQLLDDHFGLHGLVFRRTFA 
               
               
                   
                 
                   Umbellularia 
                 
                 IRSYEVGPDRSTSILAVMNHMQEATLNHAKSVGILGDGFGTTLEMSKRDLMWVVRR 
               
               
                   
                 
                   californica 
                 
                 THVAVERYPTWGDTVEVECWIGASGNNGMRRDFLVRDCKTGEILTRCTSLSVLMNT 
               
               
                   
                   
                 RTRRLSTIPDEVRGEIGPAFIDNVAVKDDEIKKLQKLNDSTADYIQGGLTPRWNDL 
               
               
                   
                   
                 DVNQHVNNLKYVAWVFETVPDSIFESHHISSFTLEYRRECTRDSVLRSLTTVSGGS 
               
               
                   
                   
                 SEAGLVCDHLLQLEGGSEVLRARTEWRPKLTDSFRGISVIPAEPRV 
               
               
                   
               
               
                 14 
                 fatBmat(fatB 
                 MEWKPKPKLPQLLDDHFGLHGLVFRRTFAIRSYEVGPDRSTSILAVMNHMQEATLN 
               
               
                   
                 without 
                 HAKSVGILGDGFGTTLEMSKRDLMWVVRRTHVAVERYPTWGDTVEVECWIGASGNN 
               
               
                   
                 leader 
                 GMRRDFLVRDCKTGEILTRCTSLSVLMNTRTRRLSTIPDEVRGEIGPAFIDNVAVK 
               
               
                   
                 sequence) 
                 DDEIKKLQKLNDSTADYIQGGLTPRWNDLDVNQHVNNLKYVAWVFETVPDSIFESH 
               
               
                   
                 
                   Umbellularia 
                 
                 HISSFTLEYRRECTRDSVLRSLTTVSGGSSEAGLVCDHLLQLEGGSEVLRARTEWR 
               
               
                   
                 
                   californica 
                 
                 PKLTDSFRGISVIPAEPRV 
               
               
                   
               
               
                 15 
                 fatB2(C8 C10 
                 MVAAAASSAFFPVPAPGASPKPGKFGNWPSSLSPSFKPKSIPNGGFQVKANDSAHP 
               
               
                   
                 fatty acid) 
                 KANGSAVSLKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMH 
               
               
                   
                 
                   Cuphea 
                 
                 DRKSKRPDMLVDSFGLESTVQDGLVFRQSFSIRSYEIGTDRTASIETLMNHLQETS 
               
               
                   
                 
                   hookeriana 
                 
                 LNHCKSTGILLDGFGRTLEMCKRDLIWVVIKMQIKVNRYPAWGDTVEINTRFSRLG 
               
               
                   
                   
                 KIGMGRDWLISDCNTGEILVRATSAYAMMNQKTRRLSKLPYEVHQEIVPLFVDSPV 
               
               
                   
                   
                 IEDSDLKVHKFKVKTGDSIQKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLET 
               
               
                   
                   
                 QELCSLALEYRRECGRDSVLESVTAMDPSKVGVRSQYQHLLRLEDGTAIVNGATEW 
               
               
                   
                   
                 RPKNAGANGAISTGKTSNGNSVS 
               
               
                   
               
               
                 16 
                 fatB2mat(fatB 
                 MDRKSKRPDMLVDSFGLESTVQDGLVFRQSFSIRSYEIGTDRTASIETLMNHLQET 
               
               
                   
                 2 without 
                 SLNHCKSTGILLDGFGRTLEMCKRDLIWVVIKMQIKVNRYPAWGDTVEINTRFSRL 
               
               
                   
                 leader 
                 GKIGMGRDWLISDCNTGEILVRATSAYAMMNQKTRRLSKLPYEVHQEIVPLFVDSP 
               
               
                   
                 sequence) 
                 VIEDSDLKVHKFKVKTGDSIQKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLE 
               
               
                   
                 
                   Cuphea 
                 
                 TQELCSLALEYRRECGRDSVLESVTAMDPSKVGVRSQYQHLLRLEDGTAIVNGATE 
               
               
                   
                 
                   hookeriana 
                 
                 WRPKNAGANGAISTGKTSNGNSVS 
               
               
                   
               
               
                 17 
                 kivd 
                 MYTVGDYLLDRLHELGIEEIFGVPGDYNLQFLDQIISRKDMKWVGNANELNASYMA 
               
               
                   
                 
                   Lactococcus 
                 
                 DGYARTKKAAAFLTTFGVGELSAVNGLAGSYAENLPVVEIVGSPTSKVQNEGKFVH 
               
               
                   
                 
                   lactis 
                 
                 HTLADGDFKHFMKMHEPVTAARTLLTAENATVEIDRVLSALLKERKPVYINLPVDV 
               
               
                   
                   
                 AAAKAEKPSLPLKKENPTSNTSDQEILNKIQESLKNAKKPIVITGHEIISFGLENT 
               
               
                   
                   
                 VTQFISKTKLPITTLNFGKSSVDETLPSFLGIYNGKLSEPNLKEFVESADFILMLG 
               
               
                   
                   
                 VKLTDSSTGAFTHHLNENKMISLNIDEGKIFNESIQNFDFESLISSLLDLSGIEYK 
               
               
                   
                   
                 GKYIDKKQEDFVPSNALLSQDRLWQAVENLTQSNETIVAEQGTSFFGASSIFLKPK 
               
               
                   
                   
                 SHFIGQPLWGSIGYTFPAALGSQIADKESRHLLFIGDGSLQLTVQELGLAIREKIN 
               
               
                   
                   
                 PICFIINNDGYTVEREIHGPNQSYNDIPMWNYSKLPESFGATEERVVSKIVRTENE 
               
               
                   
                   
                 FVSVMKEAQADPNRMYWIELVLAKEDAPKVLKKMGKLFAEQNKS 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 2 
               
               
                   
                   
               
               
                   
                 General 
                 Enzyme 
                   
                   
                   
                   
                 Accession 
               
               
                   
                 Enzyme Activity 
                 Activity 
                 Enzyme 
                 EC # 
                 Gene Name 
                 Organism 
                 Number 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 1 
                 Alkane 
                 An aldehyde + 
                 alkane 
                 4.1.99.5 
                 adm 
                   Cyanothece  sp. 
                 YP_001802195 
               
               
                   
                 deformylative 
                 O2 + 2 NADPH + 
                 deformylative 
                   
                   
                 ATCC 51142 
               
               
                   
                 monooxygenase 
                 2 H+ = an (n- 
                 monooxygenase 
                   
                 adm 
                 
                   Nostoc 
                 
                 YP_001865325 
               
               
                   
                 activity 
                 1) alkane + 
                   
                   
                   
                 
                   punctiforme 
                 
               
               
                   
                   
                 formate + H2O + 
                   
                   
                 adm 
                 
                   Prochlorococcus 
                 
                 YP_397029 
               
               
                   
                   
                 2 NADP+ 
                   
                   
                   
                   marinus  MIT 
               
               
                   
                   
                   
                   
                   
                   
                 9312 
               
               
                   
                   
                   
                   
                   
                 adm 
                 
                   Thermosynechococcus 
                 
                 NP_682103 
               
               
                   
                   
                   
                   
                   
                   
                   elongatus  BP-1 
               
               
                 2 
                 Carboxylic acid 
                 An aldehyde + 
                 carboxylic acid 
                 1.2.99.6 
                 carB 
                 
                   Mycobacterium 
                 
                 YP_889972 
               
               
                   
                 reductase activity 
                 acceptor + H2O = 
                 reductase 
                   
                   
                   smegmatis  str. 
               
               
                   
                   
                 a carboxylate + 
                   
                   
                   
                 MC2 155 
               
               
                   
                   
                 reduced 
                   
                   
                 car 
                 
                   Nocardia 
                 
                 AAR91681 
               
               
                   
                   
                 acceptor 
                   
                   
                   
                 
                   iowensis 
                 
               
               
                   
                   
                   
                   
                   
                 fadD9 
                 
                   Mycobacterium 
                 
                 YP_001850422 
               
               
                   
                   
                   
                   
                   
                   
                   marinum  M 
               
               
                 3 
                 Phosphopanthetheinyl 
                 CoA-[4′- 
                 phosphopanthetheinyl 
                 2.7.8.7 
                 entD 
                 
                   Escherichia coli 
                 
                 NP_415115 
               
               
                   
                 transferase 
                 phosphopantetheine] + 
                 transferase 
                   
                 sfp 
                 
                   Bacillus subtilis 
                 
                 ZP_12673024 
               
               
                   
                 activity 
                 apo- 
                   
                   
                   
                 subsp.  subtilis  str. SC-8 
               
               
                   
                   
                 [acyl-carrier 
               
               
                   
                   
                 protein] = 
               
               
                   
                   
                 adenosine 3′,5′- 
               
               
                   
                   
                 bisphosphate + 
               
               
                   
                   
                 holo-[acyl- 
               
               
                   
                   
                 carrier protein] 
               
               
                 4 
                 Thioesterase 
                 A fatty acyl- 
                 thioesterase 
                 3.1.2.14 
                 fatB2 
                 
                   Cuphea 
                 
                 AAC49269 
               
               
                   
                 activity 
                 [acyl-carrier 
                   
                   
                   
                 
                   hookeriana 
                 
               
               
                   
                   
                 protein] + H2O = 
                   
                   
                 tesA 
                 
                   Escherichia coli 
                 
                 NP_415027 
               
               
                   
                   
                 [acyl-carrier 
                   
                   
                 FatB3 
                 
                   Cocos nucifera 
                 
                 AEM72521 
               
               
                   
                   
                 protein] + a 
                   
                   
                 Ua- 
                 
                   Ulmus americana 
                 
                 AAB71731 
               
               
                   
                   
                 fatty acid 
                   
                   
                 FatB1 
               
               
                 5 
                 Long-chain acyl- 
                 An aldehyde + 
                 long-chain acyl- 
                 1.2.1.50 
                 acrM 
                   Acinetobacter  sp. 
                 BAB85476 
               
               
                   
                 CoA reductase 
                 CoA + NADP+ = 
                 CoA reductase 
                   
                   
                 M-1 
               
               
                   
                 activity 
                 an acyl-CoA + 
                   
                   
                 ucpA 
                 
                   Escherichia coli 
                 
                 NP_416921 
               
               
                   
                   
                 NADPH + H+ 
                   
                   
                 ybbO 
                 
                   Escherichia coli 
                 
                 NP_415026 
               
               
                   
                   
                   
                   
                   
                 luxC 
                 
                   Photorhabdus 
                 
                 NP_929340 
               
               
                   
                   
                   
                   
                   
                   
                 
                   luminescens 
                 
               
               
                   
                   
                   
                   
                   
                   
                 subsp.  laumondii   
               
               
                   
                   
                   
                   
                   
                   
                 TTO1 
               
               
                   
                   
                   
                   
                   
                 acr1 
                   Acinetobacter  sp. 
                 YP_047869 
               
               
                   
                   
                   
                   
                   
                   
                 ADP-1 
               
               
                 6 
                 Long-chain fatty 
                 ATP + a long- 
                 long-chain fatty 
                 6.2.1.3 
                 fadD 
                 
                   Escherichia coli 
                 
                 NP_416319 
               
               
                   
                 acid CoA-ligase 
                 chain fatty acid + 
                 acid CoA-ligase 
                   
                 fadD 
                 
                   Synechococcus 
                 
                 YP_001733936 
               
               
                   
                 activity 
                 CoA = AMP + 
                   
                   
                   
                 
                   elongatus 
                 
               
               
                   
                   
                 diphosphate + 
                   
                   
                 TTC0079 
                 
                   Thermus 
                 
                 YP_004054 
               
               
                   
                   
                 an acyl-CoA 
                   
                   
                   
                 
                   thermophilus 
                 
               
               
                   
                   
                   
                   
                   
                   
                 HB27