Abstract:
Anthracycline antibiotics of the formula: ##STR1## wherein R is --CO--CH 2  OH, --CHOH--CH 3 , --CO--CH 3  or --CH 2  --CH 3  having antitumor and antibacterial activity are prepared by culturing a new mutant strain of microorganism designated as Micromonospora peucetica sp. nova and which has been deposited with the American Type Culture Collection under number No. 31366 ATCC.

Description:
BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to anthracycline antibiotics which are 11-deoxy analogues of the known anthracycline antibiotics daunomycin and adriamycin*, their aglycones, methods of preparing same, the use thereof, a new strain of microorganism used to make said antibiotics and a method of making the new strain of microorganism. 
    
     2. The Prior Art 
     Daunomycin and adriamycin are known antitumor antibiotics of the anthracycline series. Their aglycones are, of course, also known. 
     The microorganism, Streptomyces peucetius var. caesius, is also known; see, Arcamone et al., Biotechnol. Biogen., 1969, X1, 1101-1110. 
     SUMMARY OF THE INVENTION 
     The invention provides, in one aspect thereof, a new class of anthracycline antibiotics, hereinafter designated as glycosides A, B, C and D, which are 11-deoxy derivatives of daunomycin and adriamycin, as well as their respective aglycones. The new 11-deoxy derivatives have the following formula: ##STR2## wherein R is --COCH 2  OH(I), --CHOH--CH 3  (II), --CO--CH 3  (III) or --CH 2  --CH 3  (IV). 
     Glycoside A has the formula (I) and is 11-deoxyadriamycin; 
     Glycoside B has the formula (II) and is 11-deoxy-13-dihydrodaunomycin; 
     Glycoside C has the formula (III) and is 11-deoxydaunomycin; and 
     Glycoside D has the formula (IV) and is 11-deoxy-13-deoxo-daunomycin. 
     The respective agylcones which are obtained from the glycosides by aqueous acid hydrolysis to remove the sugar moiety, i.e., daunosamine have the formula: ##STR3## wherein R is --COCH 2  OH(V), --CHOH--CH 3  (VI), --CO--CH 3  (VII) or --CH 2  --CH 3  (VIII). 
     The aglycone of glycoside A has the formula (V) and is 11-deoxy-adriamycinone; 
     The aglycone of glycoside B has the formula (VI) and is 11-deoxy-13-dihydro-daunomycinone; 
     The aglycone of glycoside C has the formula (VII) and is 11-deoxy-daunomycinone; and 
     The aglycone of glycoside D has the formula (VIII) and is 11deoxy-13-deoxo-daunomycinone. 
     In another aspect, the invention provides methods of preparing the glycosides A, B, C and D which method comprises culturing a new mutant strain of the microorganism Streptomyces peucetius var. caesius which produces the glycosides A, B, C and D, and recovering same from the culture medium. 
     In yet another aspect, the invention provides a method for producing the new mutant strain of said microorganism which comprises subjecting same to a mutagenic treatment with N-methyl-N&#39;-nitro-N-nitrosoguanidine. 
     In still another aspect, the invention provides said new mutant strain of the microorganism which has been designated number B211 F.I.* of the Farmitalia Collection of Microorganisms. This new mutant strain has been demonstrated to be a new species of the genus Micromonospora. It has also been designated as Micromonospora peucetica sp. nova. 
    
     In still another aspect, the invention provides pharmaceutical compositions comprising said glycosides A, B, C or D in combination with an inert carrier therefor. 
     Finally, the invention provides methods of treating certain mammalian tumors (L 1210 and P 388 leukemia) and bacterial infections using the new glycosides. 
     By way of summary then, the present invention includes the new anthracycline glycoside antibiotics designated A, B, C and D, both in dilute form as crude concentrates and in pure crystalline form, and the corresponding aglycones. The invention also includes processes for the production of these compounds by fermentation of a mutant strain of Streptomyces peucetius var. caesius, and their recovery, concentration, purification and salt preparation. 
     The new anthracycline glycosides display antitumor and antibacterial activity. More particularly, glycosides A and C are useful as antitumor agents in experimental animals. 
     The new microorganism used in the invention is obtained by a mutagenic treatment with N-methyl-N&#39;-nitro-N-nitrosoguanidine of Streptomyces peucetius var. caesius. As in the case of many antibiotic-producing cultures, fermentation of strain B211 F.I. results in the production of a mixture or a complex of components. Four bioactive anthracycline components the glycosides A, B, C and D, have been separated from that complex. 
     The present anthracycline glycosides all contain daunosamine (3-amino-2,3,6-trideoxy-L-lyxo-hexose), which is the amino sugar component of daunomycin and adriamycin (F. Arcamone, G. Cassinelli, P. Orezzi, G. Franceschi and R. Mondelli, J.Am.Chem.Soc., 86, 5335, 1964 and U.S. Pat. No. 3,590,028). The structures of the new glycosides were determined by analysis of their infrared, ultraviolet, visible, mass and magnetic resonance spectra and are all in agreement with the chemical and physical data provided hereinbelow. 
     The new anthracycline glycoside complex and its individual components form salts with both acids and bases, and pharmaceutically acceptable salts of the complex and its components are also included within the scope of the invention. Examples of such pharmaceutically acceptable salts include salts with acids such as hydrochloric, sulphuric, nitric, and phosphoric, and with metallic cations, for example, alkali metal or alkaline earth metal cations such as sodium, potassium, calcium and magnesium, and also with other cations such as trivalent iron cations. 
     DETAILED DESCRIPTION OF THE INVENTION 
     The Microorganism 
     Microscopic properties of strain B 211 F.I. 
     The spores of B 211 F.I. are of the following size: 0.9-1.1×1.1-1.6μ. They are borne singly or very frequently in pairs, and only rarely as clusters, terminally on short sporophors arising monopodially as branches on long, randomly produced hyphae 0.5-0.9μ in thickness. Rarely are sessile spores observed. No polymorphic forms are produced and aerial mycelium is absent. 
     Macroscopic properties of strain B 211 F.I. 
     The cultural characteristics of strain B 211 F.I. are given below in Table 1. Growth of the microorganism is generally good on organic media, not quite as good on synthetic media; on the former, it is generally raised and ridged, rather moist but non-slimy in appearance. 
     The sporulation layer turns the orange-terra cotta colored vegetative mycelium brown to almost black ageing. No soluble pigment is generally produced. No growth is observed at temperatures above 40° C. The physiological and biochemical properties of strain B 211 F.I. are given below in Table 2. 
     Identification and classification of strain B 211 F.I. 
     The strain B 211 F.I. is clearly referrable to the genus Micromonospora Orskov (Orskov, J. &#34;Investigations into the Morphology of the Ray Fungi&#34;, Levin and Maunksgaard, Copenhagen, 1923, page 171) of the order Actinomycetales, on the basis of its morphological and cultural characteristics. However, careful comparative examination of the characteristic described and reported by Waksman, S. A. (&#34;The Actinomycetes&#34; Vol. 2-1961, Williams and Wilkins Co. Baltimore and &#34;The Actinomycetes; A Summary of Current Knowledge&#34;, 1967, Ronald Press Co.), Luedemann and Brodsky (1963) and Luedemann, G. (&#34;Micromonospora Taxonomy&#34; Advances in Applied Microbiology, 1970, 11, 101-133) for known species belonging to this genus, with those shown by strain B 211 F.I. did not enable us to identify the present strain with any of the named species listed for the above-mentioned genus. Because of its peculiar carbohydrate utilization pattern, and because it produces new anthracyclines, it may be concluded that strain B 211 F.I. differs from all previously described species of this genus, and is a new species. This new species has been given the binomial designation of Micromonospora peucetica sp. nova. 
     
                                           TABLE 1__________________________________________________________________________Cultural characteristics of Strain B 211 F.I. on various media accordingto Waksman, 1961,supra, where not specified otherwise.Medium                Response__________________________________________________________________________Bennett&#39;s agar        Growth good, raised and ridged, color orange to                 terra cotta                 or coral red, turning to black on ageing.Emerson&#39;s agar        Growth fair, raised and ridged. Colorless to light                 terra                 cotta throughout turning brown on ageing.Glucose-asparagine agar                 Growth good, flat, color light terra cotta                 throughout.1% NZ-amine type A, 1% glycose + agar                 Growth good, raised and ridged. Color from orange                 terra(LUEDEMANN G.M. et al. Antimicrobial                 cotta to bright red, turning brown to black on                 ageing.Agents, 1963, pp. 116-124)Glucose-yeast extract agar                 Growth poor, raised and ridged. Color light terra                 cotta                 throughout.Starch-casein agar    Growth good, flat. Color terra cotta throughout.Gelatin agar          Growth poor, raised and ridged. Color terra cotta                 to                 bright red.Tyrosine agar (GORDON, R.D. and SMITH,                 Growth good, raised and ridged. Bright terra cottaM.L., J. Bacteriol., 69, 1955, pp.                 color at first, deep reddish turning copper brown                 to147-150)              almost black, on ageing. A deep brown soluble                 pigment                 is produced.Yeast extract-L-tyrosine agar                 Growth fair flat. Color brown, a deep reddish to                 coffee                 brown soluble pigment is produced.Pepton-iron agar (TRESNER, H.D. and                 Growth good, raised and ridged. Color light terra                 cottaDANGA, F., J. Bacteriol. 76, 1958,                 throughout.pp. 239-244)Potato-glucose agar   Growth moderate, raised and ridged. Color from                 terra                 cotta to brown turning almost black on ageing.Czapeck&#39;s agar        Growth poor, flat. Color light terra cotta turning                 black on ageing.Glycerol-glycine agar Growth moderate, flat. At first colorless, turning                 olive                 green to deep green on ageing.Inorganic-salts-starch agar                 Growth fair, flat. Color from rose to terra cotta(PRIDHAM, T.C. et al, Antibiotics                 turning orange brown on ageing.Annual 1956/1957, pp. 947-953)Glycerol-asparagine agar                 Growth poor, flat. Color light terra cotta turning                 almost black on ageing.SA agar (see for composition main-                 Growth good, raised and ridged. Color at first                 creamytenance medium in Example 1)                 to honey-like turning later terra cotta and                 becoming                 slightly brownish on ageing. Sometimes a yellow                 soluble                 pigment turning slightly brown on ageing is                 observed.__________________________________________________________________________ 
    
     
                       TABLE 2______________________________________ Physiological and biochemical properties of Strain B 211______________________________________F.I.*Utilization of:      glucose        +      sucrose        +      D-xylose       +      M-mannitol     +      M-inositol     +      L-arabinose    +      D-fructose     +      Adonitol       +      lactose        +      d(+) mannose   +      maltose        +      raffinose      +      L-rhamnose     +      alpha-alpha-trehalose                     +      esculin        +      glycerol       +      Na-citrate     +      NH.sub.4 -succinate                     +      Na-acetate     +      NH.sub.4 -tartrate                     +      glycogen       +      paraffin       -Negative control      -Liquefaction of gelatin                 +Tyrosine decomposition                 +Malanin formation     +Hydrolysis of starch  +H.sub.2 S formation   -Nitrate reduction     +Milk (pepton- and coag.)Antibiotics produced: new anthracyclines______________________________________ + = positive reaction - = negative reaction *The medium for the carbohydrate utilization test is as described by R.D. Gordon and M.L. Smith, supra. *The media used for the other physiological reactions are those reported by S.A. Waksman, 1961, supra. 
    
     FERMENTATION PROCESS 
     The production of the new glycosides is carried out by conventional, well known methods, and comprises culturing the microorganism in a previously sterilized liquid culture medium under aerobic conditions at a temperature of from 25° C. to 37° C. (preferably at 28° C.) for from 5 to 30 days (preferably 15 days) and at a pH which initially is from 6.5 to 7.0 and which, at the end of the fermentation period is from 6.5 to 8.0. 
     The culture medium consists of a carbon and a nitrogen source as well as mineral salts. The carbon source may, for example, be starch, dextrin, glucose, glycerin, mannite, maltose, corn steep liquor, distillers solubles, soybean oil or soybean meal. The nitrogen source, besides those of the above-mentioned complex substances which contain nitrogen, may be for example, dry yeast, meat pepton or casein. Good results are even obtained by using ammonium salts such as ammonium nitrate, ammonium sulphates and diammonium phosphates. The mineral salts vary according to the medium employed. In a medium containing complex substances such as various meals and fermentation residues, the addition of calcium carbonate and sodium or potassium phosphates has proved useful. In media containing glucose, or ammonium salts, much higher levels of mineral salts such as potassium, sodium or calcium salts, and additions of trace elements such as iron, zinc, copper, magnesium and manganese are necessary. The fermentation may be carried out in Erlenmeyer flasks or in laboratory or industrial fermenters of various capacities. 
     ANALYTICAL METHODS 
     Samples of the fermentation broth and crude preparations are subjected to paper chromatography using Whatman No. 1 paper, buffered with M/15 phosphate buffer at pH 5.4, employing as the eluant a mixture of n-propanol:ethyl acetate:water (7:1:2). The paper strips are bioautographed against Bacillus subtilis, and four antibiotic components were found to occur. These are designated glycoside A (Rf 0.30), B (Rf 0.50), C (Rf 0.55) and D (Rf 0.65). 
     Crude preparations are subjected to thin layer chromatography (TLC) using pre-coated TLC plates of silica gel 60-F-254 (Merck) and as the eluant, a mixture of chloroform:methanol:acetic acid:water (80:20:14:6). Four yellow compounds occur, and they correspond to glycoside A (Rf 0.50), B (Rf 0.55), C (Rf 0.65) and D (Rf 0.70) respectively. A quantitative estimation of the total yellow constituents present in the fermentation broth can be made by the following method. To a sample of broth, adjusted to pH 8.6, two volumes of chloroform:methanol (9:1) are added. The resulting mixture is sonicated for 1 minute at room temperature. On a sample of the organic phase, diluted with acidic methanol, the total content of the yellow anthracyclines and of their aglycones can be spectrophotometrically determined at 418 nm. On a sample of the organic phase, concentrated under reduced pressure, quantitative determination of the single glycosides can be obtained by preparative TLC using the above reported system. The different yellow zones are scraped off and eluted with methanol. Each constituent is spectrophotometrically determinated at 418 nm. Glycoside A is usually the major constituent in the fermentation broths. 
     ISOLATION PROCEDURE 
     After the fermentation, the active compounds are contained in the mycelia and in the fermentation liquor. The anthracycline antibiotic complex can be extracted at pH 8.5-9.0  in the form of the free bases from the culture broth &#34;in toto&#34; with a water immiscible organic solvent such as butanol, methyl isobutyl ketone, chloroform, methylene dichloride or ethyl acetate. Preferably, the mycelia and the fermentation liquor are separated by filtration at pH 4 with the aid of diatomaceous earth, and then extracted separately. 
     The filtration cake is extracted with a mixture of a water-soluble solvent, such as acetone, methanol or other lower alcohol, and an 0.1 N aqueous solution of an inorganic or organic acid, such as hydrochloric acid, sulphuric acid or acetic acid. Generally, a mixture of acetone: 0.1 N hydrochloric acid in a ratio 4:1 by volume is employed. The mycelia extracts are collected, adjusted to pH 4, and then concentrated under reduced pressure. The aqueous concentrate is combined with the filtered broth, adjusted to pH 8.5-9.0, then extracted with a water immiscible organic solvent, preferably chloroform or n-butanol. The extracts are concentrated under reduced pressure and the anthracycline complex is precipitated by addition of five volumes of n-hexane. The constituents of the crude complex are then fractionated and purified by column chromatography. 
     PURIFICATION PROCEDURE 
     Further purification of the antibiotic complex and its separation into its four components may be effected by silica gel column chromatography. The crude orange brown powder is dissolved in chloroform and the solution, mixed with an equivalent of methanolic hydrogen chloride, is chromatographed on silica gel with chloroform:methanol:water mixtures. The components D and C are eluted first, with a 94.8:5.0:0.2 mixture. The glycosides B and A follow with an 89.5:10.0:0.5 mixture. The components are usually separated as shown by paper and thin layer chromatography and the four components are obtained as their hydrochlorides in crystalline form. 
     CHEMICAL AND PHYSICAL PROPERTIES 
     The novel antibiotics of the invention have some common properties, but they can be distinguished on the basis of their chemical and physical characteristics. All the new anthracyclines have comparable solubility; as free bases they are soluble in chloroform, methylene dichloride, acetone, methanol, ethanol, aqueous alcohols, acidic water, dioxane and pyridine, but sparingly soluble or insoluble in diethyl ether, n-hexane, cyclohexane and petroleum ether. As hydrochlorides, they are soluble in water, methanol, ethanol and aqueous alcohols, but insoluble in acetone, benzene, chloroform, diethyl ether and pertroleum ether. They can be used as indicators, being orange-yellow in neutral and acidic solutions, in which they also show orange-red fluorescence under U.V. light. Their alkaline solutions are red-brown, and when treated with alcoholic magnesium acetate they form red solutions. All these properties and the absorption spectra in the ultraviolet and visible regions, indicate that these new compounds are anthracycline antibiotics. The chemical and physical properties of glycosides A, B, C and D, isolated as the hydrochlorides are given in Table 3. 
     
                                           TABLE 3__________________________________________________________________________Chemical and physical properties of Glycosides A, B, C and DPROPERTY   GLYCOSIDE A. HCl               GLYCOSIDE B. HCl                           GLYCOSIDE C. HCl                                       GLYCOSIDE D. HClMelting Point   171°-173° (dec.)               163°-164° (dec.)                           175°-176° (dec.)                                       140°-150°__________________________________________________________________________                                       (dec.)[α] 23°(c = 0.2 inmethanol)   +111°               +107°                           +139°                                       +122°U.V. andVisible SpectraλMeOH max   228,260,418 nm               228,260,418 nm                           228,260,418 nm                                       228,260,418 nm 1% 1 cm   645,420,193 640,410,179 713,450,199 610,395,171λpH 7 buffer max   235,262,426 nm               235,262,246 nm                           235,262,426 nm                                       235,262,426 nm 1%E 1 cm   600,406,161 605,400,160 617,424,166 580,400,160λ0.01 N NaOH max   510 nm      510 nm      510 nm      510 nm 1%E 1 cm   120         118         128         110I.R. Spectrum   3,700-2,400            1,200               3,700-2,400                        1,195                           3,700-2,300                                    1,195                                       3,700-2,400                                                1,19(KBr):cm.sup.-1   1,725    1,180               1,665    1,180                           1,710                1,18   1,670    1,115               1,625    1,115                           1,670    1,115                                       1,665    1,11   1,625    1,085               1,585       1,625       1,625    1,04   1,585    1,055               1,485    1,050                           1,585    1,085                                       1,585    1,01   1,490    1,015               1,470    1,010                           1,490    1,010                                       1,485    98   1,470    985               1,445    985                           1,470    985                                       1,465    83   1,450    940               1,420    940                           1,445    835                                       1,445    75   1,420    875               1,385    840                           1,420    815                                       1,420    43   1,385    840               1,325    820                           1,385    795                                       1,385   1,330    820               1,290    750                           1,325    750                                       1,325   1,300    795               1,235    435                           1,295    435                                       1,295   1,290    755            1,235       1,255   1,240    440                        1,235Empirical   C.sub.27 H.sub.29 NO.sub.10 . HCl               C.sub.27 H.sub.31 NO.sub.9 . HCl                           C.sub.27 H.sub.29 NO.sub.9 .                                       C.sub.27 H.sub.31 NO.sub.8 .                                       HClFormulaMolecularWeight  564         550         548         534__________________________________________________________________________ 
    
     The new anthracycline antibiotics also show characteristic proton magnetic resonance (PMR) spectra. The spectrum of glycoside A, as the hydrochloride in DMSO-d 6 , showed characteristic signals at 1.14δ (d, CH 3  --C--5&#39;), 3.39δ (s, CH 3  O), 4.60δ (broad s, C--14--H 2 ) 4.89δ (broad s, C--7--H), 5.27δ (broad s, C--1&#39;--H), 7.23δ (s, C--11--H), 7.4-7.9δ (m, C--1--H, C--2--N and C--3--H) and 13.61δ (s, C--6--OH) glycoside C, as the hydrochloride in DMSO-d 6 , showed signals at 1.15δ (d, CH 3  --C--5&#39;), 2.26δ (s, CH 3  CO), 3.92δ (s, CH 3  O), 4.90δ (broad s, C--7--H), 5.26δ (broad s, C--1&#39;--H) 7.31δ (s, C-- 11--H), 7.4-7.9δ (m, C--1--H, C--2H, and C--3--H) and 13.65δ (s, C--6--OH). 
     Field desorption mass spectroscopy of the free bases of glycosides A, B, C and D confirmed the assigned molecular formulae as shown in Table 4. 
     
                       TABLE 4______________________________________              Molecular WeightCompound   Molecular Formula                    Calculated                              Found: m/e______________________________________GLYCOSIDE A      C.sub.27 H.sub.29 NO.sub.10                    527       527 (M.sup.+)GLYCOSIDE B      C.sub.27 H.sub.31 NO.sub.9                    513       514 (MH.sup.+)GLYCOSIDE C      C.sub.27 H.sub.29 NO.sub.9                    511       512 (MH.sup.+)GLYCOSIDE D      C.sub.27 H.sub.31 NO.sub.8                    497       498 (MH.sup.+)______________________________________ 
    
     Aqueous acid hydrolysis of glycosides A, B, C and D gives four different water insoluble yellow aglycones of the formulae V-VIII, above. The aqueous soluble fractions all contained the same reducing amino-sugar which was identified as daunosamine (3-amino-2,3,6-trideoxy-L-lyxohexose), the amino sugar component of daunomycin and adriamycin. 
     The structures of the aglycones were determined by analysis of their infrared, ultraviolet, visible, mass and magnetic resonance spectra. Their chemical and physical properties are reported in Table 5. 
     
                                           TABLE 5__________________________________________________________________________Chemical and physical properties of the aglycones of glycosides A, B, Cand DPROPERTY  AGLYCONE OF A                AGLYCONE OF B                           AGLYCONE OF C                                      AGLYCONE OF D__________________________________________________________________________Melting point °C.     220        175-180 with                           213-215    175-180 with                decomposition         decomposition[α].sub.D.sup.23° (c = 0.1)     +161°          +144°                                      +164°     (dioxan)              (methanol) (methanol)U.V. e VIS SpectraγMeOH max     227,259,418 nm                227,258,418 nm                           227,258,418 nm                                      227,258,418 nm 1%E 1 cm    895,640,268           930,660,317                                      990,640,280Empirical formula     C.sub.21 H.sub.18 O.sub.8                C.sub.21 H.sub.20 O.sub.7                           C.sub.21 H.sub.18 O.sub.7                                      C.sub.21 H.sub.20 O.sub.6Molecular weightcalculated     398        384        382        368Found m/e 398 (M.sup.+)                384 (M.sup.+)                           382 (M.sup.+)                                      368 (M.sup.+)__________________________________________________________________________ 
    
     BIOLOGICAL ACTIVITY DATA 
     (a) Antibacterial activity 
     The &#34;in vitro&#34; minimum inhibitory concentrations (MIC) of glycosides A, B, C and D were determined for certain microorganisms using the standard tube dilution procedure and are reported in Table 6. 
     
                       TABLE 6______________________________________     MIC in μg/ml     GlycosidesTest Organism       A (I)    B (II)   C (III)                                D (IV)______________________________________Staphylococcus       125      1000     62     250aureus 209 PStaphylococcus       500      1000     250    1000aureus 153Sarcina lutea       100      100        12.5  25ATCC 9341Bacillus subtilis       100      100      50     100ATCC 6633Escherichia coli B        50      100      25      50______________________________________ 
    
     (b) Antitumor activity 
     The new glycosides were tested against HeLa cells in vitro (time of exposure to the drugs: 24 hours), and on L 1210 and P 388 leukemia in mice in comparison with daunomycin. The results of the in vitro tests are given in Table 7. It can be seen therefrom that all the compounds inhibited the cell viability of HeLa cells in vitro, the ID 50  being 0.05 μg/ml for glycoside C, 0.1 μg/ml for glycoside A, 0.22 μg/ml for glycoside D and 0.44 μg/ml for glycoside B. 
     The in vivo data obtained in mice are reported in Table 8. Glycoside A was active against P 388 leukemia at a dose of 66 mg/kg, and toxic at a dose of 100 mg/kg. At the tolerated dose, the antitumor activity was greater than that of daunomycin. 
     
                       TABLE 7______________________________________Effect on HeLa cells viability in vitro     Dose(a)   No. of colonies(b)                             ID.sub.50Compound  (ng/ml)   %             (ng/ml)______________________________________Daunomycin     12.6      35-15          6-7     6.25      48-66     3.12      71-80Glycoside A     200       1(I)       100       63             105     50        73     25        74Glycoside C     200       3(III)     100       44             54     50        54Glycoside D     400       0(IV)      200       53             220     100       109Glycoside B     1600      0(II)      800       20             440     400       66______________________________________ (a)HeLa cells were exposed to the drugs for 24 hours, then plated. Number of colonies was evaluated 5 days later. (b)% over untreated controls. 
    
     
                       TABLE 8______________________________________Effect against P 388 and L 1210 leukemia in mice   Dose (a)           T/C%           Toxic (b)Compound  (mg/kg)   L 1210    P 388  deaths______________________________________Daunomycin     2.9       133-133   190     4.4       140-133   200     6.6       111-133   223    14/26Glycoside A     2.9       111(I)       6.6       128     10        122     66                  245     100                 254     1/10Glycoside C     2.9       100(III)     6.6       117     15        122     100                 181    3/8     150                  45     3/19Glycoside D     2.9       100(IV)      6.6       100     15        111     125                  18    5/5     250        0         9     5/5Glycoside B     2.9       100(II)      6.6       122     15        122     500                  9     2/2______________________________________ (a)Mice were treated intraperitoneally on day 1 after tumor cell inoculation. (b)Evaluated on the basis of the macroscopic autopsy findings. 
    
    
    
     The following Examples are further illustrative of the invention. 
     EXAMPLE 1 
     A culture of Micromonospora peucetica, strain B 211 F.I., was grown for 25 days at 28° C. on agar slants of maintenance medium SA. Medium SA has the following composition: glucose 3%; brewers dry yeast 1.2%; sodium chloride 0.1%; potassium dihydrogen orthophosphate 0.05%; calcium carbonate 0.1% magnesium sulphate 0.005%; ferrous sulphate heptahydrate 0.0005%; zinc sulphate heptahydrate 0.0005%; cupric sulphate pentahydrate 0.0005%; agar 2%; tap water up to 100%. Sterilization was carried out by heating in an autoclave at 120° C. for 20 minutes. 
     The mycelial mat of the culture so obtained was scraped off and suspended in 3 ml of sterile distilled water. The suspension was inoculated in 300 ml Erlenmeyer flasks containing 60 ml of the following liquid growth medium: brewers dry yeast 0.3%; peptone 0.5%; calcium nitrate tetrahydrate 0.05%; tap water up to 100%. Sterilization was effected by heating in an autoclave at 120° C. for 20 minutes. The pH of the medium after sterilization was between 6.8 and 7.0. 
     The inoculated flasks were shaken for 8 days at a temperature of 28° C. on a rotary shaker running at 250 rpm and describing a circle of 7 cm in diameter. 5 ml of the culture grown as described above were inoculated in a 300 ml Erlenmeyer flask containing 50 ml. of the following production medium: glucose 6%; brewers dry yeast 3%; sodium chloride 0.2%; potassium dihydrogen orthophosphate 0.1%; calcium carbonate 0.2% magnesium sulphate 0.01%; ferrous sulphate heptahydrate 0.001%; zinc sulphate heptahydrate 0.001%; cupric sulphate pentahydrate 0.001%; tap water up to 100%; sterilization by heating in autoclave at 115° C. for 20 minutes. 
     The flasks were incubated at 28° C. for 25 days under conditions identical to those described for the seed phase. The maximum concentration of the active compounds was reached between the 18th and 22nd days of fermentation with a production of 90 mcg/ml. 
     EXAMPLE 2 
     A culture of strain B 211 F.I. was grown for 14 days as described in Example 1, and the mycelial mat, collected as described in Example 1, was inoculated in 300 ml Erlenmeyer flasks containing 50 ml of the following liquid growth medium: soluble starch, 4%; soy bean flour 1.5%; brewer&#39;s dry yeast 0.5%, corn steep liquor 0.8%; calcium carbonate 0.3%; potassium monohydrogen orthophosphate 0.05%; magnesium sulphate 0.025%; potassium chloride 0.025%; tap water up to 100%. The pH, being 5.7 was brought to 7.5 with sodium hydroxide before sterilization. Sterilization was effected at 120° C. for 20 minutes. 
     After 4 days of incubation under the same conditions as described in Example 1, 5 ml of the thus grown culture were inoculated in 300 ml Erlenmeyer flasks containing 50 ml of the medium described above. 
     The flasks were then incubated under the same conditions as described above for 10 days. The maximum concentrations of the active compounds was reached between the 8th and 9th days of fermentation with a production of 15 mcg/ml. 
     EXAMPLE 3 
     The whole beer (20 liters) from a fermentation broth obtained according to Example 1, was adjusted to a pH of about 4 with hydrochloric acid and filtered, using 3% diatomaceous earth as a filter aid, to yield a cake and a filtrate which were extracted separately. The wet filter cake was treated with about 4 liters of a mixture of acetone and 0.1 N aqueous hydrochloric acid (80:20). After filtration, a second treatment with an additional 3 liters of acidified aqueous acetone assured complete extraction of the active compounds. The combined aqueous acetone extracts were adjusted with ammonium hydroxide to a pH of about 4, concentrated under reduced pressure to about 1 liter, and combined with the filtered broth. The obtained mixture was adjusted to a pH of about 8.5 to 9.0 and then extracted twice with one-half volume of a chloroform:methanol (9:1) mixture. The combined organic extracts were washed with water, dried on anhydrous sodium sulphate, and concentrated under reduced pressure to a volume of about 200 ml. By addition of 1 liter of n-hexane, the crude complex precipitated as a yellow brown powder (7 g). 
     EXAMPLE 4 
     A chloroform solution (6 g in 200 ml) of the crude complex (free base) after treatment with 10 ml of 1 N methanolic hydrogen chloride, was placed on a column of silicic acid (prepared in chloroform). The column was washed with chloroform, followed by elution with a chloroform:methanol:water mixture (94.8:5:0.2). The first yellow fraction contained some aglycones. The next two yellow bands contained, respectively, glycosides D and C. Elution was continued with a chloroform:methanol:water mixture (89.5:10:0.5) until two other yellow bands, corresponding to glycosides B and A, were eluted. The fractions containing these four bands were separately concentrated under reduced pressure to give substantially pure hydrochlorides of glycoside D (0.2 g), glycoside C (0.4 g), glycoside B (0.2 g) and glycoside A (0.6 g) as microcrystalline powders. Recrystallization of the glycosides A and C from methanol:n-butanol gave the corresponding pure hydrochlorides as yellow-orange crystals, m. p. 171°-173° C. with decomposition for glycoside A, m.p. 163°-164° C. with decomposition for glycoside B, m.p. 175°-176° C. with decomposition for glycoside C and m.p. 140°-150° C. with decomposition for glycoside D. 
     EXAMPLE 5 
     A 250 mg sample of glycoside A was dissolved in 10 ml of 0.2 N aqueous hydrochloric acid and the solution was heated for 1 hour at 95° C. A crystalline yellow-orange precipitate was collected by filtration after cooling, washed with water and dried over phosphorus pentoxide overnight under vacuum. 170 mg of the aglycone of glycoside A were obtained in pure form, m.p. 220° C., m/e: 398 (M + ). After precipitation of the aglycone, the almost colorless aqueous acidic solution was adjusted to pH 5 with an anion exchange resin and then freeze-dried. The residue (60 mg), crystallized from methanol:acetone gave a crystalline compound, m.p. 166° C. with decomposition, identified as daunosamine hydrochloride by direct comparison with an authentic sample. Similar treatment of glycosides B, C and D yield the respective agylcones thereof. 
     Variations and modifications can, of course, be made without departing from the spirit and scope of the invention.