Abstract:
The invention provides a latanoprost-containing aqueous pharmaceutical composition filled in a filter-equipped container, with the adsorption of latanoprost by the filter being prevented. In the latanoprost-containing aqueous pharmaceutical composition filled in the filter-equipped container, a nonionic surfactant is contained in the composition and the filter is made from at least one material selected from the group consisting of polyether sulfone, polyvinylidene fluoride, polycarbonate, and polytetrafluoroethylene.

Description:
TECHNICAL FIELD 
       [0001]    The present invention relates to an aqueous pharmaceutical composition filled in a filter-equipped container, comprising latanoprost and a nonionic surfactant. 
       BACKGROUND ART 
       [0002]    Aqueous pharmaceutical compositions including eye drops and the like are required to have sufficient preservatives-effectiveness in order to prevent them from being contaminated by microorganisms during production or use of the compositions. The preservatives-effectiveness is usually checked by the preservatives-effectiveness tests prescribed in the general information of the Japanese Pharmacopoeia. 
         [0003]    To impart sufficient preservatives-effectiveness to the aqueous pharmaceutical composition, it has been considered essential to contain the preservatives, in particular, benzethonium chloride, benzalkonium chloride, parabens, chlorobutanol, sorbic acid or their salts, or the like. However, there is a concern that use of some kinds of preservatives may have an adverse effect on some tissues, for example, the cornea or the like. Therefore, it is desirable that aqueous pharmaceutical compositions doesn&#39;t contain any kinds of preservatives, or contain them in lower concentration if we have to add some preservatives. 
         [0004]    The absence of preservative naturally suggests that the composition will not pass the preservatives-effectiveness tests. There is proposed another method where no preservative is added, i.e., a method of using as containers for holding aqueous pharmaceutical compositions therein a special container, notably, a filter-equipped container or a disposable container (single-unit container). The filter-equipped container can prevent the entry of bacteria into the contents, i.e., constitutionally prevent the contamination of the contents (JP 2004-051170 A and JP 2004-166987 A). The disposable container is discarded once opened and used, so that the polluted container will not be used again even if bacteria are mixed into the contents of the container. The method of using such a filter-equipped container or disposable container is considered remarkably advantageous as the method where no preservative is added to the aqueous pharmaceutical compositions. 
         [0005]    However, use of the disposable container will lead to loss of much liquid medication. For convenience in dividing the medication into portions, it is necessary to pack the liquid medication in each container with excess of the single quantity to be used at one time. This is highly disadvantageous, especially for the medication containing expensive raw materials. In addition, some disposable containers are in special shapes or others have to be treated differently from general containers, for example, by discarding the first one drop of medication prior to the use. This may cause various problems in use. In contrast to this, the filter-equipped container has the advantage of being treated in the same manner as in the general containers. This means the users can treat the filter-equipped container without recognizing the filter-equipped container as a special one, which can significantly reduce unexpected troubles. 
         [0006]    As previously mentioned, it is considered extremely useful to fill a filter-equipped container with an aqueous pharmaceutical composition free of preservative that may have an adverse effect on some tissues, such as the cornea or the like. 
         [0007]    In light of the structure of the container, the aqueous pharmaceutical composition is allowed to pass through the filter installed in the container. Therefore, consideration should be given to the interaction between the ingredients contained in the aqueous pharmaceutical composition and the filter installed in the container. There are some materials that tend to be easily adsorbed by the filter due to their chemical or physical properties. In other words, the compatibility between the aqueous pharmaceutical composition and the material for constituting the filter is important when the aqueous pharmaceutical composition is filled in the filter-equipped container. 
       SUMMARY OF INVENTION 
     Technical Problem 
       [0008]    An object of the present invention is to provide a latanoprost-containing aqueous pharmaceutical composition filled in a container equipped with a filter, with adsorption of latanoprost to the filter being prevented. 
       Solution to Problem 
       [0009]    The inventors of the present invention intensively investigated the effects of additives contained in the aqueous pharmaceutical composition on the adsorption of latanoprost to the filter. As a result, it has been found that the adsorption of latanoprost to the filter can be significantly prevented when the composition contains a nonionic surfactant and is filled in a container equipped with a filter constructed from a particular material. The invention has thus accomplished. 
         [0010]    Accordingly, the invention provides an aqueous pharmaceutical composition shown below. 
         [0011]    1. A latanoprost-containing aqueous pharmaceutical composition filled in a container with a filter, characterized in that the composition further comprises a nonionic surfactant and the filter comprises at least one material selected from the group consisting of polyether sulfone, polyvinylidene fluoride, polycarbonate, and polytetrafluoroethylene. 
         [0012]    2. The aqueous pharmaceutical composition as described in the above-mentioned item 1, wherein the material for the filter is polyether sulfone. 
         [0013]    3. The aqueous pharmaceutical composition as described in the above-mentioned item 1 or 2, wherein the nonionic surfactant comprises at least one selected from the group consisting of polysorbate, polyoxyethylene hydrogenated castor oil, polyoxyl stearate, and polyoxyethylene polyoxypropylene glycol. 
         [0014]    4. The aqueous pharmaceutical composition as described in the above-mentioned item 1 or 2, wherein the nonionic surfactant comprises at least one selected from the group consisting of polysorbate 80, polyoxyethylene hydrogenated castor oil 60, polyoxyl (40) stearate, and polyoxyethylene (200) polyoxypropylene glycol (70). 
         [0015]    5. The aqueous pharmaceutical composition as described in the above-mentioned item 1 or 2, wherein the nonionic surfactant comprises polysorbate 80 and polyoxyethylene hydrogenated castor oil 60. 
       ADVANTAGEOUS EFFECTS OF INVENTION 
       [0016]    In the latanoprost-containing aqueous pharmaceutical composition filled in a filter-equipped container according to the invention, a nonionic surfactant is contained in the composition and the filter employs a specific material. This has the effect of significantly preventing latanoprost from being adsorbed by the filter. 
     
    
     DESCRIPTION OF EMBODIMENTS 
       [0017]    The concentration of latanoprost employed in the composition of the invention, which is not particularly limited as long as the effects of the invention can be obtained, may be preferably 0.0005 to 5 w/v %, and more preferably 0.001 to 1 w/v %. 
         [0018]    Any nonionic surfactant(s) can be used in the invention with no particular limitation as long as the effects of the invention can be obtained. Preferably, polysorbate, polyoxyethylene hydrogenated castor oil, polyoxyl stearate and polyoxyethylene polyoxypropylene glycol may be used, and more preferably polysorbate 80, polyoxyethylene hydrogenated castor oil 60, polyoxyl 40 stearate and polyoxyethylene (200) polyoxypropylene glycol (70) may be used, and most preferably polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 may be used. 
         [0019]    The concentration of the nonionic surfactant used in the invention is not particularly limited as long as the effects of the invention can be obtained, but may be preferably in the range of 0.0005 to 10 w/v %, and more preferably 0.001 to 5 w/v %. 
         [0020]    The material of the container used in the invention is not particularly limited as long as the effects of the invention can be obtained, but may be preferably polypropylene, polyethylene or polyethylene terephthalate, and more preferably polypropylene. 
         [0021]    The material of the filter used in the invention is not particularly limited as long as the effects of the invention can be obtained, but preferably may be polyether sulfone (PES), polyvinylidene fluoride (PVDF), polycarbonate, polytetrafluoroethylene (PTFE) or mixed cellulose ester (MSE). In particular, polyether sulfone is more preferable. The commercially available filters made from polyether sulfone include Millipore Express (registered trademark) and Millipore Express (registered trademark) PLUS made by Millipore Corporation, and the like. 
         [0022]    There is no limit to pH of the aqueous pharmaceutical composition of the invention as long as the effects of the invention can be obtained, but the pH range of 4 to 10 is preferable. In consideration of irritation to the eyes, the aqueous pharmaceutical composition of the invention may be preferably adjusted to pH 5.0 to 8.0, and more preferably 6.2 to 7.2. 
         [0023]    Any pH adjustor can be used with no particular limitation, and phosphates and borates can be used, for example. More specifically, sodium dihydrogen phosphate dihydrate, disodium phosphate dodecahydrate, boric acid, borax and the like can be used. 
         [0024]    The osmotic pressure ratio of the aqueous pharmaceutical composition of the invention is not particularly limited as long as the effects of the invention can be obtained, but preferably in the range of 0.1 to 4.0. In consideration of irritation to the eyes, the osmotic pressure ratio may be preferably adjusted to 0.5 to 2.0, and more preferably 0.8 to 1.2. 
         [0025]    To adjust the osmotic pressure ratio of the aqueous pharmaceutical composition according to the invention, a variety of isotonic agents generally used may be added. Examples of the ionic isotonic agent include potassium chloride, calcium chloride, sodium chloride, magnesium chloride and the like. Examples of the nonionic isotonic agent include dextrose, xylitol, D-sorbitol, D-mannitol, glycerol, propylene glycol, macrogol 4000 and the like. 
         [0026]    The aqueous pharmaceutical composition according to the invention may further comprise sodium edetate or the like as a stabilizer, preferably in an amount of 0.0001 to 0.1 w/v %, and more preferably 0.001 to 0.01 w/v %. 
         [0027]    The latanoprost-containing aqueous pharmaceutical composition filled in a filter-equipped container according to the invention is adaptable to any administration route by taking advantage of the characteristics of the composition. The compositions adaptable to local administration to the ears, nose or eyes are desirable, and the compositions prepared for the local administration to the eyes (i.e., eye drops) are most preferable. 
       EXAMPLES 
     Test Example 1 
       [0028]    0.02 g of benzalkonium chloride was added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper (material: polypropylene) equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition was prepared (Comparative Example 1). 
         [0029]    Either polysorbate 80 or polyoxyethylene hydrogenated castor oil 60 was added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, aqueous pharmaceutical compositions of the invention were prepared (Examples 1 and 2). 
         [0030]    The content of latanoprost in each of the aqueous pharmaceutical compositions prepared in Comparative Example and Examples mentioned above was determined by high-performance liquid chromatography (HPLC). The determination was carried out using three samples: the first drop which was allowed to pass through the filter and drop (hereinafter referred to as “first drop”) after the aqueous pharmaceutical composition was prepared and filled in the container, the subsequent drop (hereinafter referred to as “second drop”), and a solution filled in the container (hereinafter referred to as “solution in container”). The adsorption of latanoprost by the filter was evaluated by comparing the HPLC results of the first drop and second drop with solution in container. The results are shown in Table 1. 
         [0000]    
       
         
               
               
               
               
             
               
               
               
               
             
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                   
                 Comparative 
                   
                   
               
               
                 Ingredients (w/v (%)) 
                 Example 1 
                 Example 1 
                 Example 2 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Latanoprost 
                 0.005 
                 0.005 
                 0.005 
               
               
                 Sodium dihydrogen phosphate dihydrate 
                 0.585 
                 0.585 
                 0.585 
               
               
                 Disodium phosphate dodecahydrate 
                 1.2 
                 1.2 
                 1.2 
               
               
                 Benzalkonium chloride 
                 0.02 
                 — 
                 — 
               
               
                 Polysorbate 80 
                 — 
                 0.5 
                 — 
               
               
                 Polyoxyethylene hydrogenated castor oil 
                 — 
                 — 
                 0.5 
               
               
                 60 
               
               
                 pH of composition 
                 6.7 
                 6.7 
                 6.7 
               
               
                 Osmotic pressure ratio of composition 
                 approx. 1 
                 approx. 1 
                 approx. 1 
               
             
          
           
               
                 Material for filter: polyether sulfone 
                   
               
             
          
           
               
                 Content of 
                 Solution in container 
                 96.7% 
                 103.6% 
                 104.5%  
               
               
                 Latanoprost 
                 First drop 
                 47.4% 
                  93.1% 
                 93.7% 
               
               
                 (ratio (%) 
                 (vs. solution in container) 
                 (decrease 
                 (decrease 
                 (decrease 
               
               
                 based on the 
                   
                 of 50.9%) 
                 of 10.1%) 
                 of 10.3%) 
               
               
                 concentration of 
                 Second drop 
                 28.7% 
                 105.6% 
                 97.7% 
               
               
                 composition) 
                 (vs. solution in container) 
                 (decrease of 
                 (decrease of 
                 (decrease of 
               
               
                   
                   
                 70.3%) 
                 1.9%) 
                 6.5%) 
               
               
                   
               
             
          
         
       
     
         [0031]    When Examples 1 and 2 are compared with Comparative Example 1, it is found that the adsorption of latanoprost to the filter can be remarkably reduced by adding the nonionic surfactant of the invention to the composition. The decrease of content of latanoprost in the first drop is about 10% in each of Examples 1 and 2. The decrease of the content of latanoprost in the second drop is −1.9% in Example 1. The decrease of the content of latanoprost in the second drop is 6.5% in Example 2. Both in first drop and in second drop, the decreases of the content of latanoprost in Example 1 and Example 2 are lower than that in Comparative Example 1. Those results demonstrate that each nonionic surfactant, polysorbate 80 or polyoxyethylene hydrogenated castor oil 60, can have the effect of the invention. 
       Test Example 21 
       [0032]    0.02 g of benzalkonium chloride was added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition was prepared (Comparative Example 2). 
         [0033]    Polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition of the invention was prepared (Example 3). 
         [0034]    Polysorbate 80, polyoxyethylene hydrogenated castor oil 60 and polyoxyl 40 stearate were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition of the invention was prepared (Example 4). 
         [0035]    Polysorbate 80, polyoxyethylene hydrogenated castor oil 60 and propylene glycol were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition of the invention was prepared (Example 5). 
         [0036]    Polysorbate 80, polyoxyethylene hydrogenated castor oil 60, polyoxyl 40 stearate and polyoxyethylene (200) polyoxypropylene glycol (70) were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition of the invention was prepared (Example 6). 
         [0037]    The content of latanoprost in each of the aqueous pharmaceutical compositions prepared in Comparative Example and Examples mentioned above was determined by HPLC. The determination was carried out using three samples: the first drop which was allowed to pass through the filter and drop (hereinafter referred to as “first drop”) after the aqueous pharmaceutical composition was prepared and filled in the container, the subsequent drop (hereinafter referred to as “second drop”), and a solution packed in the container (hereinafter referred to as “solution in container”). The adsorption of latanoprost by the filter was evaluated by comparing the HPLC results of the first drop and second drop with solution in container. The results are shown in Table 2. 
         [0000]    
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 2 
               
               
                   
                   
               
               
                   
                 Comparative 
                 Example 
                 Example 
                 Example 
                 Example 
               
               
                   
                 Example 2 
                 3 
                 4 
                 5 
                 6 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 Ingredients (w/v(%)) 
                   
                   
                   
                   
                   
               
               
                 Latanoprost 
                 0.005 
                 0.005 
                 0.005 
                 0.005 
                 0.005 
               
               
                 Sodium dihydrogen 
                 0.585 
                 0.585 
                 0.585 
                 0.293 
                 0.585 
               
               
                 phosphate dihydrate 
               
               
                 Disodium phosphate 
                 1.2 
                 1.2 
                 1.2 
                 0.6 
                 1.2 
               
               
                 dodecahydrate 
               
               
                 Benzalkonium chloride 
                 0.02 
                 — 
                 — 
                 — 
                 — 
               
               
                 Polysorbate 80 
                 — 
                 0.5 
                 0.5 
                 0.5 
                 0.5 
               
               
                 Polyoxyethylene 
                 — 
                 0.5 
                 0.5 
                 0.5 
                 0.5 
               
               
                 hydrogenated castor oil 60 
               
               
                 Polyoxyl 40 stearate 
                 — 
                 — 
                 0.08 
                 — 
                 0.08 
               
               
                 Polyoxyethylene (200) 
                 — 
                 — 
                 — 
                 — 
                 5 
               
               
                 polyoxypropylene glycol (70) 
               
               
                 Propylene glycol 
                 — 
                 — 
                 — 
                 1.4 
                 — 
               
               
                 pH of composition 
                 6.7 
                 6.7 
                 6.7 
                 6.7 
                 6.7 
               
               
                 Osmotic pressure ratio of 
                 approx. 1 
                 approx. 1 
                 approx. 1 
                 approx. 1 
                 approx. 1 
               
               
                 composition 
               
               
                 Material for filter: 
               
               
                 polyether sulfone 
               
             
          
           
               
                 Content of 
                 Solution in 
                 96.7% 
                 99.4% 
                 96.5% 
                 97.5% 
                 103.3% 
               
               
                 Latanoprost 
                 container 
               
               
                 (ratio (%) 
                 First drop 
                 47.4% 
                 93.0% 
                 85.4% 
                 84.1% 
                 99.0% 
               
               
                 based on the 
                 (vs. solution 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
               
               
                 concentration 
                 in container) 
                 of 50.9%) 
                 of 6.4%) 
                 of 11.5%) 
                 of 13.7%) 
                 of 4.2%) 
               
               
                 of composition 
                 Second drop 
                 28.7% 
                 96.7% 
                 91.6% 
                 96.6% 
                 101.9% 
               
               
                   
                 (vs. solution 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
               
               
                   
                 in container) 
                 of 70.3%) 
                 of 2.7%) 
                 of 5.0%) 
                 of 0.9%) 
                 of 1.4%) 
               
               
                   
               
             
          
         
       
     
         [0038]    When Examples 3 to 6 are compared with Comparative Example 2, it is found that the adsorption of latanoprost by the filter can be remarkably reduced by adding the nonionic surfactant of the invention to the composition. The decrease of the content of latanoprost in the first drop is 4.2 to 13.7%, and in the second drop is 0.9 to 5.0% in Examples 3 to 6. The rate of decrease in the latanoprost content in any of Examples 3 to 6 is obviously lower than that in Comparative Example 2. 
       Test Example 3 
       [0039]    Polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, Sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper (material: polypropylene) equipped with a filter made from mixed cellulose ester (MF-Millipore (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition was prepared (Comparative Example 3). 
         [0040]    Polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper (material: polypropylene) equipped with a filter made from polyethylene (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition was prepared (Comparative Example 4). 
         [0041]    Polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition of the invention was prepared (Example 7). 
         [0042]    The content of latanoprost in each of the aqueous pharmaceutical compositions prepared in Comparative Examples and Example mentioned above was determined by HPLC. The determination was carried out using three samples: the first drop which was allowed to pass through the filter and drop (hereinafter referred to as “first drop”) after the aqueous pharmaceutical composition was prepared and filled in the container, the subsequent drop (hereinafter referred to as “second drop”), and a solution filled in the container (hereinafter referred to as “solution in container”). The adsorption of latanoprost by the filter was evaluated by comparing the HPLC results of the first drop and second drop with “solution in container. The results are shown in Table 3. 
         [0000]    
       
         
               
               
               
               
             
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 3 
               
               
                   
               
               
                   
                 Comparative 
                 Comparative 
                   
               
               
                 Ingredients (w/v (%)) 
                 Example 3 
                 Example 4 
                 Example 7 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Latanoprost 
                 0.005 
                 0.005 
                 0.005 
               
               
                 Sodium dihydrogen phosphate 
                 0.585 
                 0.585 
                 0.585 
               
               
                 dihydrate 
               
               
                 Disodium phosphate 
                 1.2 
                 1.2 
                 1.2 
               
               
                 dodecahydrate 
               
               
                 Polysorbate 80 
                 0.5 
                 0.5 
                 0.5 
               
               
                 Polyoxyethylene hydrogenated 
                 0.5 
                 0.5 
                 0.5 
               
               
                 castor oil 60 
               
               
                 pH of composition 
                 6.7 
                 6.7 
                 6.7 
               
               
                 Osmotic pressure ratio of 
                 approx. 1 
                 approx. 1 
                 approx. 1 
               
               
                 composition 
               
               
                 Material for filter 
                 Mixed cellulose 
                 Polyethylene 
                 Polyether sulfone 
               
               
                   
                 ester 
               
             
          
           
               
                 Content of 
                 Solution in 
                 95.1% 
                 97.4% 
                 99.4% 
               
               
                 Latanoprost 
                 container 
               
               
                 (ratio (%) 
                 First drop 
                 61.0% 
                  0.0% 
                 93.0% 
               
               
                 based on the 
                 (vs. solution in 
                 (decrease of 
                 (decrease of 
                 (decrease of 
               
               
                 concentration 
                 container) 
                 35.8%) 
                 100%) 
                 6.4%) 
               
               
                 of 
                 Second drop 
                 81.1% 
                 94.6% 
                 96.7% 
               
               
                 composition) 
                 (vs. solution in 
                 (decrease of 
                 (decrease of 2.9%) 
                 (decrease of 
               
               
                   
                 container) 
                 14.7%) 
                   
                 2.7%) 
               
               
                   
               
             
          
         
       
     
         [0043]    When Example 7 is compared with Comparative Examples 3 and 4, it is found that the adsorption of latanoprost by the filter can be remarkably reduced by using the specific material of the filter of the invention. The rate of decrease in the latanoprost content in the first and second drops in Example 7 is obviously lower than that in Comparative Examples 3 and 4. Those results demonstrate that use of the filter especially made from polyether sulfone can have the significant effects of the invention. 
       Test Example 4 
       [0044]    0.02 g of benzalkonium chloride was added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition was prepared (Comparative Example 5). 
         [0045]    Polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition of the invention was prepared (Example 8). 
         [0046]    Polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, boric acid and borax were separately added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, an aqueous pharmaceutical composition of the invention was prepared (Example 9). 
         [0047]    Polysorbate 80 and polyoxyethylene hydrogenated castor oil 60 were added to 50 mL of sterile purified water and dissolved therein. To this solution 0.005 g of latanoprost was added. Then, the combination of sodium dihydrogen phosphate dihydrate and disodium phosphate dodecahydrate, or the combination of boric acid and borax was individually added, and sodium edetate was further added. After the whole solution was confirmed to be clear, sterile purified water was added until the total volume reached 100 mL. The solution thus obtained was transferred to an eye dropper equipped with a filter made from polyether sulfone (Millipore Express (registered trademark) made by Millipore Corporation) and the eye dropper was tightly sealed. Thus, aqueous pharmaceutical compositions of the invention were prepared (Examples 10 and 11). 
         [0048]    The content of medication in each of the aqueous pharmaceutical compositions prepared in Comparative Example and Examples mentioned above was determined by HPLC. The determination was carried out using three samples: the first drop which was allowed to pass through the filter and drop (hereinafter referred to as “first drop”) after the aqueous pharmaceutical composition was prepared and filled in the container, the subsequent drop (hereinafter referred to as “second drop”), and a solution filled in the container (hereinafter referred to as “solution in container”). The adsorption of latanoprost by the filter was evaluated by comparing the HPLC results of the first drop and second drop with solution in container. The results are shown in Table 4. 
         [0000]    
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 4 
               
               
                   
                   
               
               
                   
                 Comparative 
                 Example 
                 Example 
                 Example 
                 Example 
               
               
                   
                 Example 5 
                 8 
                 9 
                 10 
                 11 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 Ingredients 
                   
                   
                   
                   
                   
               
               
                 Latanoprost 
                 0.005 
                 0.005 
                 0.005 
                 0.005 
                 0.005 
               
               
                 Sodium dihydrogen 
                 0.585 
                 0.585 
                 — 
                 0.585 
                 — 
               
               
                 phosphate dihydrate 
               
               
                 Disodium phosphate 
                 1.2 
                 1.2 
                 — 
                 1.2 
                 — 
               
               
                 dodecahydrate 
               
               
                 Boric acid 
                 — 
                 — 
                 1.2 
                 — 
                 1.2 
               
               
                 Borax 
                 — 
                 — 
                 0.06 
                 — 
                 0.06 
               
               
                 Benzalkonium chloride 
                 0.02 
                 — 
                 — 
                 — 
                 — 
               
               
                 Polysorbate 80 
                 — 
                 0.5 
                 0.5 
                 0.5 
                 0.5 
               
               
                 Polyoxyethylene 
                 — 
                 0.5 
                 0.5 
                 0.5 
                 0.5 
               
               
                 hydrogenated castor oil 60 
               
               
                 Disodium edetate 
                 — 
                 — 
                 — 
                 0.005 
                 0.005 
               
               
                 pH of composition 
                 6.7 
                 6.7 
                 6.7 
                 6.7 
                 6.7 
               
               
                 Osmotic pressure ratio of 
                 approx. 1 
                 approx. 1 
                 approx. 1 
                 approx. 1 
                 approx. 1 
               
               
                 composition 
               
               
                 Material for filter: 
               
               
                 polyether sulfone 
               
             
          
           
               
                 Content of 
                 Solution in 
                 96.7% 
                 99.4% 
                 97.7% 
                 97.1% 
                 98.2% 
               
               
                 Latanoprost 
                 container 
               
               
                 (ratio (%) 
                 First drop 
                 47.4% 
                 93.0% 
                 86.8% 
                 81.0% 
                 81.5% 
               
               
                 based on the 
                 (vs. solution 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
               
               
                 concentration 
                 in container) 
                 of 50.9%) 
                 of 6.4%) 
                 of 11.1%) 
                 of 16.5%) 
                 of 17.0%) 
               
               
                 of composition 
                 Second drop 
                 28.7% 
                 96.7% 
                 93.2% 
                 91.6% 
                 91.1% 
               
               
                   
                 (vs. solution 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
                 (decrease 
               
               
                   
                 in container) 
                 of 70.3%) 
                 of 2.7%) 
                 of 4.6%) 
                 of 5,6%) 
                 of 7.2%) 
               
               
                   
               
             
          
         
       
     
         [0049]    When Examples 8 and 9 are compared with Comparative Example 5, it is found that the adsorption of latanoprost by the filter can be remarkably reduced by adding the nonionic surfactant to the composition. The decrease of content of latanoprost is 6.4 to 11.1% in the first drop; and less than 5% in the second drop, in Examples 8 and 9, showing that in any case the rate of decrease in the latanoprost content is lower than that in Comparative Example 5. When the results of Example 8 are compared with those of Example 9, there is virtually no difference in the adsorption of latanoprost to the filter. It is found that the effects of the invention can be obtained regardless of the kinds of buffer agents. 
         [0050]    When Examples 10 and 11 are compared with Comparative Example 5, it is found that the adsorption of latanoprost to the filter can be remarkably reduced by adding the nonionic surfactants to the composition. Under such circumstances, the effects of the invention can be maintained even though the composition further comprises the stabilizer such as sodium edetate, which is accepted as a pharmaceutical additive. 
       FORMULATION EXAMPLES 
       [0051]    The formulation examples in addition to the above-mentioned examples are shown below. 
       Formulation Example 1 
       [0052]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Latanoprost 
                 0.005 (w/v %)  
               
               
                   
                 Sodium dihydrogen phosphate dihydrate 
                 0.3 (w/v %) 
               
               
                   
                 Disodium phosphate dodecahydrate 
                 0.6 (w/v %) 
               
               
                   
                 Polysorbate 80 
                 0.5 (w/v %) 
               
               
                   
                 Polyoxyethylene hydrogenated castor oil 60 
                 0.5 (w/v %) 
               
               
                   
                 Propylene glycol 
                 1.4 (w/v %) 
               
               
                   
                 Sodium edetate 
                 0.005 (w/v %)  
               
               
                   
                   
               
             
          
         
       
     
       Formulation Example 2 
       [0053]      
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Latanoprost 
                 0.005 (w/v %)  
               
               
                   
                 Boric acid 
                 1.2 (w/v %) 
               
               
                   
                 Borax 
                 0.06 (w/v %)  
               
               
                   
                 Polysorbate 80 
                 0.5 (w/v %) 
               
               
                   
                 Polyoxyethylene hydrogenated castor oil 60 
                 0.5 (w/v %) 
               
               
                   
                 Propylene glycol 
                 0.5 (w/v %) 
               
               
                   
                   
               
             
          
         
       
     
         [0054]    The formulation example 1 and the formulation example 2 are each available as the composition of the eye drop by filled in the filter-equipped container.