Abstract:
Disclosed is a microchannel chip having an opening on the side of a plate with a lowered production cost. In the microchannel chip, a first plate ( 11 ), to which a third concavity ( 17 ) opening at one side surface ( 13 ) and a joining surface ( 14 ) is formed on the joining surface ( 14 ), is joined to a second plate ( 21 ), to which a sixth concavity ( 26 ) opening at one side surface ( 23 ) is formed on the joining surface and a groove ( 27′ ) interconnecting with the sixth concavity ( 26 ) is formed. The third concavity ( 17 ) and the sixth concavity ( 26 ) are aligned facing each other, and by the third concavity ( 17 ) and the sixth concavity ( 26 ), a glass tube introducing opening ( 33 ) is formed having a wider width than that of a duct ( 27 ). An adhesive agent is injected into the glass tube introducing opening ( 33 ) and a glass tube is inserted.

Description:
TECHNICAL FIELD 
       [0001]    The present invention relates to a micro flow path chip made of a resin substrate having a micro flow path therein and a micro analysis system. 
       BACKGROUND ART 
       [0002]    In a current scientific field or a medical field such as biochemistry and analytical chemistry, a micro analysis system being employed for rapidly testing and analyzing a small amount of protein or nucleic acid (for example, DNA) with accuracy. 
         [0003]    As such a micro analysis system, Patent literature 1 discloses, for example, an insert for a laboratory vessel, as a system for storing a plurality of samples, having a plurality of reception cavities into which laboratory vessels including samples can be inserted. Patent literature 1 also discloses reception cavities having flow paths for helping washing of samples in the inserted laboratory vessel or a rinse process, the flow paths having reduced diameters and opening toward a bottom. 
         [0004]    Patent literature 2 discloses a configuration of attaching a connection part to a flow plate of a multipurpose flow module and introducing fluid samples subjected to analysis into a flow path of the flow plate. 
       CITATION LIST 
     Patent Literature 
       [0005]    PLT 1 
         [0006]    Japanese Translation of a PCT Application Laid-Open No.2009-541038 
         [0007]    PLT 2 
         [0008]    Japanese Translation of a PCT Application Laid-Open No.2009-524508 
       SUMMARY OF INVENTION 
     Technical Problem 
       [0009]    However, a mold structure is complex and forming is more difficult in integral forming of resin products having a recessed part deeply recessed from an opening, such as the reception cavity disclosed in the above-described Patent literature 1, or resin products having both a recessed part provided with an opening having a relatively large cross-section area and a flow path corresponding to the recessed part and having a small cross-section area. The connection part attached to a flow plate disclosed in the above-described Patent literature 2 is a member having a tubular structure having a plurality of regions having different inner diameters. A mold structure is complex as with the laboratory vessel insert disclosed in Patent literature 1, in forming of a member having such a shape with resin or integral forming of the above-described connection part and the flow plate. In view of the above, a product having an opening on the side surface of a plate and forming a flow path corresponding to the opening causes a soaring cost of the product in association with a complex mold structure. 
         [0010]    It is an object of the present invention to provide a micro flow path chip which has an opening on the side surface of a plate and can reduce a product cost, and a micro analysis system. 
       Solution to Problem 
       [0011]    A micro flow path chip according to the present invention is a micro flow path chip including an assembly of a first thin plate and a second thin plate, further including: a first recessed part having an opening formed on a joint surface and a side surface of the first plate; and a second recessed part and a groove formed on a joint surface of the second plate, the second recessed part having an opening on the joint surface and a side surface of the second plate, the groove having a smaller size in a width direction and a depth direction than the second recessed part in a cross section parallel to the side surface, wherein: the first recessed part and the second recessed part have no undercut part such that each of the first and second recessed parts has a shape of a cross section parallel to the joint surface, and the shape of the cross section does not change or decrease as the cross section is away from the joint surface; and the first recessed part of the first plate faces and is joined to the second recessed part of the second plate to form a side opening region being a recess which opens on the side surface and a flow path formed by closing the groove with the joint surface of the first plate. 
         [0012]    The micro analysis system according to the present invention employs a configuration to include the above-described micro flow path chip. 
       Advantageous Effects of Invention 
       [0013]    According to the present invention, it is possible to provide a micro flow path chip which has an opening on the side surface of a plate and can reduce a product cost, and a micro analysis system. 
     
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         [0014]      FIG. 1  is a diagram showing the shape of the first plate configuring a micro flow path chip according to Embodiment 1 of the present invention; 
           [0015]      FIG. 2  is a diagram showing the shape of the second plate configuring the micro flow path chip according to Embodiment 1 of the present invention; 
           [0016]      FIG. 3  is a diagram showing the shape of the micro flow path chip according to Embodiment 1 of the present invention; 
           [0017]      FIG. 4  is a diagram showing the shape of the first plate configuring a micro flow path chip according to Embodiment 2 of the present invention; 
           [0018]      FIG. 5  is a diagram showing the shape of the second plate configuring the micro flow path chip according to Embodiment 2 of the present invention; 
           [0019]      FIG. 6  is a cross-section view taken by line A-A in  FIG. 4B  in a state in which first plate  41  and second plate  51  are joined; 
           [0020]      FIG. 7  is a diagram showing the shape of the first plate configuring a micro flow path chip having projecting parts according to another embodiment of the present invention; 
           [0021]      FIG. 8  is a diagram showing the shape of the second plate configuring the micro flow path chip having the projecting part according to the other embodiment of the present invention; and 
           [0022]      FIG. 9  is a diagram showing the shape of the micro flow path chip having the projecting part according to the other Embodiments of the present invention. 
       
    
    
     DESCRIPTION OF EMBODIMENTS 
       [0023]    Embodiments of the present invention will now be described in detail with reference to the drawings. 
       Embodiment 1 
       [0024]    In Embodiment 1 of the present invention, a micro flow path chip formed by joining two plates will be described. 
         [0025]      FIG. 1  is a diagram showing the shape of first plate  11  configuring a micro flow path chip according to Embodiment 1 of the present invention.  FIG. 1A  is a plane view showing the shape of first plate  11 .  FIG. 1B  is a side view showing the positions of first recessed parts  15  and second recessed parts  16  in first plate  11 .  FIG. 1C  is a side view showing the positions of third recessed parts  17  in first plate  11 .  FIG. 1D  is an enlarged view showing a part framed by dotted circle C 1  in  FIG. 1A .  FIG. 1E  is a cross-section view taken by line A-A in  FIG. 1D .  FIG. 1F  is an enlarged view showing a part framed by dotted circle C 2  in  FIG. 1A .  FIG. 1G  is a cross-section view taken by line B-B in  FIG. 1F . 
         [0026]    In order to avoid explanations in overlapping components, reference numerals are assigned to representative components and the components will be explained in drawings and the following explanations. 
         [0027]    First plate  11  is formed with a planar plate of a resin material and has pluralities of first recessed parts  15  and second recessed parts  16  which open on one side surface  12  (an upper side surface in the drawing) and joint surface  14 . 
         [0028]    First plate  11  has a plurality of third recessed parts  17  which open on the other side surface  13  (the lower side surface in the drawing) and joint surface  14  and are arranged at the positions opposite to the plurality of first recessed parts  15 . 
         [0029]    First to third recessed parts  15  to  17  have quadrangular cross-sections parallel to side surfaces  12  and  13 . Considering that first to third recessed parts  15  to  17  are recessed from joint surface  14  as a reference surface, each of these recessed parts has a bottom, an opening edge on a side surface, an edge opposite to the opening edge on the side surface, and inner walls extending from the bottom toward joint surface  14 . Each of third recessed parts  17  has a region having a larger depth (the distance from joint surface  14  to a bottom) and width (the distance between facing inner walls) compared to bottom  17   a  and inner walls  17   c  adjacent to the opening edge on the side surface, in the opposite to the opening edge on the side surface, and has bottom  17   b  and inner walls  17   c.  These recessed parts  15  to  17  have no part widening from the opening of joint surface  14  to a bottom, and the shapes of the cross-sections in the recessed parts parallel to joint surface  14  are the substantially same at any position according to the present embodiment. 
         [0030]      FIG. 2  is a diagram showing the shape of second plate  21  configuring a micro flow path chip according to Embodiment 1 of the present invention.  FIG. 2A  is a plane view showing the shape of second plate  21 .  FIG. 2B  is a side view showing the positions of fourth recessed parts  24  and fifth recessed parts  25  in second plate  21 .  FIG. 2C  is a side view showing the positions of sixth recessed parts  26  in second plate  21 .  FIG. 2D  is an enlarged view showing a part framed by dotted circle C 3  in  FIG. 2A .  FIG. 2E  is a cross-section view taken by line C-C in  FIG. 2D .  FIG. 2F  is an enlarged view showing a part framed by dotted circle C 4  in  FIG. 2A .  FIG. 2G  is a cross-section view taken by line D-D in  FIG. 2F . 
         [0031]    In order to avoid explanations in overlapping components, reference numerals are assigned to representative components and the components will be explained in drawings and the following explanation. 
         [0032]    Second plate  21  is made of a planar resin material and has pluralities of fourth recessed parts  24  and fifth recessed parts  25  which open on one side surface  22  (an upper side surface in the drawing) and joint surface  14 . 
         [0033]    Second plate  21  has a plurality of sixth recessed parts  26  which open on the other side surface  23  (the lower side surface in the drawing) and joint surface  14  and are arranged at the positions opposite to the plurality of fourth recessed parts  24 . 
         [0034]    Second plate  21  has grooves  27 ′ communicating fourth recessed parts  24  with respective sixth recessed parts  26  and grooves  28 ′ communicating fifth recessed parts  25  with respective grooves  27 ′. The widths of grooves  27 ′ and  28 ′ are narrower than those of fourth to sixth recessed parts  24  to  26 . 
         [0035]    Fourth to sixth recessed parts  24  to  26  have quadrangular cross-sections parallel to side surfaces  22  and  23 . When fourth to sixth recessed parts  24  to  26  are recessed from joint surface  14  as a reference surface, each of these recessed parts has a bottom, an opening edge on a side surface, an edge opposite to the opening edge on the side surface, and inner walls extending from the bottom toward joint surface  14 . Each of sixth recessed parts  26  has a region having a larger depth (the distance from joint surface  14  to a bottom) and width (the distance between facing inner walls) compared to bottom  26   a  and inner walls  26   c  adjacent to the opening edge on the side surface, in the opposite to the opening edge on the side surface, and has bottom  26   b  and inner walls  26   c.  These recessed parts  24  to  26  have no part widening from the opening of joint surface  14  to a bottom, and the shapes of cross-sections in the recessed parts parallel to joint surface  14  are the substantially same at any position according to the present embodiment. 
         [0036]      FIG. 3  is a diagram showing the shape of micro flow path chip  30  according to Embodiment 1 of the present invention.  FIG. 3A  is a plane view showing the shape of micro flow path chip  30 .  FIG. 3B  is a side view showing positions of fluid introducing ports  31  as side opening regions in micro flow path chip  30 .  FIG. 3C  is a side view showing positions of glass tube introducing ports  33  as side opening regions in micro flow path chip  30 .  FIG. 3D  is an enlarged view showing a part framed by dotted circle C 5  in  FIG. 3A .  FIG. 3E  is a cross-section view taken by line E-E in  FIG. 3D .  FIG. 3F  is an enlarged view showing a part framed by dotted circle C 6  in  FIG. 3A .  FIG. 3G  is a cross-section view taken by line F-F in  FIG. 3F . 
         [0037]    Micro flow path chip  30  is formed by joining first plate  11  shown in  FIG. 1  to second plate  21  shown in  FIG. 2  on joint surface  14 . First recessed parts  15 , second recessed parts  16 , and third recessed parts  17  in first plate  11  respectively face fourth recessed parts  24 , fifth recessed parts  25 , and sixth recessed parts  26  in second plate  21 . First recessed parts  15  and fourth recessed parts  24  form respective fluid introducing ports  31  as side opening regions. Third recessed parts  17  and sixth recessed parts  26  form glass tube introducing ports  33  as side opening regions and connection parts  34  as wider regions connecting glass tube introducing ports  33  to flow paths  27 . 
         [0038]    Openings of groove  27 ′ and groove  28 ′ are closed by joint surface  14  of first plate  11  to form flow paths  27  and  28 . 
         [0039]    First plate  11  and second plate  21  are joined, for example, through adhesion with an organic adhesive, and thermal compression bond. 
         [0040]    First plate  11  and second plate  21  are formed of resin material having a high light-permeability such as acryl, polycarbonate, and polyolefin, and are desirably made of the same materials. 
         [0041]    A glass tube is inserted into the glass tube introducing port and then the appropriate amount of an adhesive is injected into a gap between the glass tube and the inner wall of the glass tube introducing port. The injected adhesive is introduced into the back of glass tube introducing port  33  by capillary attraction. The adhesive flows in the gap between the glass tube and the inner wall of glass tube introducing port  33 , is introduced into the back of glass tube introducing port  33 , and reaches the entry of connection part  34 . At this time, the adhesive flowing into connection part  34  can be blocked by capillary repulsion since the gap between the glass tube and the inner wall of connection part  34  is drastically expanded. Accordingly, the inserted glass tube can be fixed without a flow of the adhesive into a flow path. 
         [0042]    According to Embodiment 1, a micro flow path chip is formed by dividing the micro flow path chip into two plates in the thickness direction of a plate and joining the joint surfaces of the two plates. The micro flow path chip has side opening regions which have openings on the side surface of the plate and flow paths in communication with the side opening regions. This can reduce a manufacturing cost of the micro flow path chip. Accordingly, forming a recessed part with two divided plates can reduce the depth from the joint surface in the recessed part, compared to a case of forming a side opening region in one plate as one recessed part. This can reduce the height of protrusion as well in the surface forming a cavity of a mold. Accordingly, it is possible to make manufacturing of mold pieces and forming of a plate easier. When a shallow recessed part obtained by dividing a side opening region into two regions and a groove forming a flow path are formed on the same joint surface, mold pieces for the joint surface can be integrated in a complex shape. The part corresponding to the recessed part and the groove can be formed through the same process such as electrocasting. A joint surface of a micro flow path chip which is formed using an integrated piece can obtain a higher positional accuracy than a joint surface formed by combining a plurality of pieces. 
         [0043]    When the side opening region is used as a fluid introducing port as shown in Embodiment 1, the side opening region formed by second recessed part  16  of first plate  11  and fifth recessed part  25  of second plate  21  can be used as an outlet for discharging air. In other words, it is possible to discharge air in a flow path which is excluded by introduction of fluid injected from a fluid introducing port, to the exterior from the outlet for discharging air. 
         [0044]    Although it is easy to join two recessed parts obtained by dividing each of side opening regions which have large widths, it is difficult to determine a position when dividing each of flow paths having small widths into two and joining them, in a micro flow path chip having pluralities of side opening regions and flow paths. However, it is possible to easily determine a position in the joint of two plates by forming a side opening region which has a larger size and width in the direction of plate thickness than those of a flow path, with two divided plates, forming recessed parts on a joint surface, and forming a groove forming the flow path in only one of the plates, according to Embodiment 1 of the present invention. 
         [0045]    According to Embodiment 1, a micro flow path chip has a side opening region having an opening on a side surface of a plate, a wider region having a larger cross-section area parallel to the side surface than an area of the opening, and a flow path in communication with the side opening region through the wider region, and is formed by dividing the micro flow path chip into two plates in the thickness direction of a plate and joining joint surfaces of the two plates. This can reduce a manufacturing cost of the micro flow path chip. Accordingly, forming a recessed part by dividing each of side opening region and the wider region into two on the two plates can reduce the depth of the recessed part from a joint surface compared to a case of forming each of the side opening region and the wider region in one plate as one recessed part. This can reduce the height of protrusion as well in a surface forming cavity of a mold, thereby making the manufacturing of mold pieces and forming of a plate easier. When a shallow recessed part obtained by dividing each of the side opening region and the wider region into two and a groove forming a flow path are formed on the same joint surface, mold pieces for the joint surface can be integrated in a complex shape. The part corresponding to the recessed part and the groove can be formed through the same process such as electrocasting. A joint surface of a micro flow path chip which is formed using an integrated piece can obtain a higher positional accuracy than a joint surface formed by combining a plurality of pieces. 
         [0046]    When the side opening region is used as a glass tube introducing port as shown in Embodiment 1, it is possible to prevent the flow of the adhesive injected in a gap between an inner wall of the side opening region and a glass tube in the wider region. Consequently, entry of the adhesive into the flow path can be prevented. 
         [0047]    It is difficult to integrally form a micro flow path chip having a wider region having a large cross-section area parallel to a side surface between a side opening region and a flow path. However, according to the present invention, such a micro flow path chip can be easily formed by joining two plates. 
       Embodiment 2 
       [0048]    A case has been described where the side opening region formed on the side surface of the plate is used as a glass tube introducing port in Embodiment 1. A case will be described where the side opening region is used as an optical fiber introducing port, assuming that fluorescence in adjacent points in multi-point detection are simultaneously measured in Embodiment 2. 
         [0049]      FIG. 4  is a diagram showing the shape of first plate  41  configuring a micro flow path chip according to Embodiment 2 of the present invention.  FIG. 4A  is a plane view showing the shape of first plate  41 .  FIG. 4B  is an enlarged view showing a part framed by dotted circle C 7  in  FIG. 4A .  FIG. 4C  is a cross-section view taken by line A-A in  FIG. 4B . 
         [0050]    In order to avoid explanations in overlapping components, reference numerals are assigned to representative components and the components will be explained in drawings and the following explanation. 
         [0051]    First plate  41  has a plurality of first recessed parts  44  which open on one side surface  42  (the side surface in the left side in the drawing) and joint surface  43 . Each of first recessed parts  44  is a triangle of which the width gradually decreases from one side surface  42  to the center, and communicates with rectangular groove  44   a,  around the apex in the part of triangle of which the width decreases. 
         [0052]    First plate  41  has grooves  45 ′, each groove  45 ′ adjoining to the apex of first recessed part  44 , and having through holes  46 ′ and  47 ′ respectively corresponding to ports  46  and  47  for injecting samples and migration solution in both ends of groove  45 ′. 
         [0053]      FIG. 5  is a diagram showing the shape of second plate  51  configuring a micro flow path chip according to Embodiment  2  of the present invention.  FIG. 5A  is a plane view showing the shape of second plate  51 .  FIG. 5B  is an enlarged view showing a part framed by dotted circle C 8  in  FIG. 5A .  FIG. 5C  is a cross-section view taken by line B-B in  FIG. 5B . 
         [0054]    In order to avoid explanations in overlapping components, reference numerals are assigned to representative components and the components will be explained in drawings and the following explanation. 
         [0055]    Second plate  51  has a plurality of second recessed parts  53  which open on one side surface  52  (the side surface in the left side in the drawing) and joint surface  43 . Each of second recessed parts  53  is a triangle of which the width gradually decreases from the one side surface to the center, and communicates with rectangular groove  53   a , around the apex in the part of triangle of which the width decreases. 
         [0056]      FIG. 6  is a cross-section view taken by line A-A in  FIG. 4B  in a state in which first plate  41  and second plate  51  are joined. 
         [0057]    A micro flow path chip is formed by joining first plate  41  shown in  FIG. 4  to second plate  51  shown in  FIG. 5 . First recessed parts  44  of first plate  41  face respective second recessed parts  53  of second plate  51 . First recessed parts  44  and second recessed parts  53  form respective optical fiber introducing ports  61  as side opening regions. Grooves  45 ′ and through holes  46 ′ and  47 ′ are closed by the joint surface of second plate  51  to form respective flow paths  45  and ports  46  and  47  respectively. 
         [0058]    According to Embodiment 2, a micro flow path chip is formed by dividing the micro flow path chip into two plates in the thickness direction of a plate and joining the joint surfaces of two plates. The micro flow path chip has side opening regions which have openings on the side surface of the plate and flow paths located close to the side opening regions. This can reduce a manufacturing cost of the micro flow path chip. Accordingly, forming a recessed part with two divided plates can reduce the depth from a joint surface in the recessed part, compared to a case of forming the side opening region in one plate as one recessed part. This can reduce the height of protrusion as well in the surface forming a cavity of a mold, thereby making manufacturing of mold pieces and forming of a plate easier. When a shallow recessed part obtained by dividing a side opening region into two regions and a groove forming a flow path are formed on the same joint surface, mold pieces for the joint surface can be integrated in a complex shape. The part corresponding to the recessed part and the groove can be formed through the same process such as electrocasting. A joint surface of a micro flow path chip which is formed using an integrated piece can obtain a higher positional accuracy than a joint surface formed by combining a plurality of pieces. 
         [0059]    When the side opening region is used as an optical fiber insert as shown in Embodiment 2, positions of a detecting portion in a flow path and an end of an optical fiber can be determined with high accuracy. 
       Another Embodiment 
       [0060]    The present invention is applicable besides the glass tube introducing port and the optical fiber introducing port. The side opening region provided in a plate may be formed on the side surface of the plate as a projecting part and used as a tube connecter as shown  FIGS. 7 to 9 . 
         [0061]      FIG. 7  is a diagram showing the shape of first plate  71  configuring a micro flow path chip having a projecting part according to another embodiment of the present invention.  FIG. 7A  is a plane view showing the shape of first plate  71 .  FIG. 7B  is an enlarged view showing a part framed by dotted circle C 9  in  FIG. 7A .  FIG. 7C  is a cross-section view taken by line A-A in  FIG. 7B . 
         [0062]      FIG. 8  is a diagram showing the shape of second plate  81  configuring a micro flow path chip having the projecting part according to the other embodiment of the present invention.  FIG. 8A  is a plane view showing the shape of second plate  81 .  FIG. 8B  is an enlarged view showing a part framed by dotted circle C 10  in  FIG. 8A .  FIG. 8C  is a cross-section view taken by line B-B in  FIG. 8B . 
         [0063]      FIG. 9  is a diagram showing the shape of micro flow path chip  90  having the projecting part according to the other embodiment of the present invention.  FIG. 9A  is a plane view showing the shape of micro flow path chip  90 .  FIG. 9B  is an enlarged view showing a part framed by dotted circle C 11  in  FIG. 9A .  FIG. 9C  is a cross-section view taken by line C-C in  FIG. 9B . 
         [0064]    In  FIGS. 7 to 9 , the projecting part of micro flow path chip  90  is tube connecter  91 . Tube connecter  91  has an opening at its end. A cross-section area parallel to a side surface of the opening of tube connecter  91  is larger than that of a flow path in communication with tube connecter  91 . Tube connecter  91  has a protruding part on its circumferential surface. The protruding part has a tapered surface so as to prevent a tube from being released when the tube connecter is inserted into the tube. 
         [0065]    According to the present embodiment, a tube connecter is formed as a side opening region by joining joint surfaces of divided first plate and second plate. The joint surface between the first plate and the second plate is recognized in the cross-section shown in  FIG. 9C . 
         [0066]    Since such a tube connecter is manufactured by two divided plates, a tube connecter having a complex irregular shape in its interior and circumference can be easily manufactured, which can reduce a manufacturing cost. 
         [0067]    For ease of understanding, the flow path in the micro flow path chip and the side opening region are illustrated by a solid line in plane views ( FIGS. 3A and 9A ) showing a micro flow path chip according to each embodiment of the present invention. 
         [0068]    In the micro flow path chips according to all embodiments of the present invention, a case has been described where a recessed part formed in each of two plates has the substantially same shape of a cross-section parallel to joint surface  72  at any position. The present invention is not limited thereto, but any shape may be employed as long as there is no irregular shape that serves as an undercut part in the direction from joint surface  72  to a bottom of the recessed part. 
         [0069]    According to all embodiments of the present invention, there has been described a micro flow path chip having pluralities of side opening regions and flow paths corresponding to the side opening regions. The present invention is not limited thereto, but the micro flow path chip may have one or more side opening regions and flow paths corresponding to the side opening regions. 
         [0070]    The disclosure of Japanese Patent Application No.2010-167227, filed on Jul. 26, 2010, including the specification, drawings and abstract, is incorporated herein by reference in its entirety. 
       INDUSTRIAL APPLICABILITY 
       [0071]    A micro flow path chip and a micro analysis system according to the present invention can be employed for an apparatus which accurately tests and analyzes a small amount of substances in a scientific field or medical field such as biochemistry and analytical chemistry. 
       REFERENCE SIGNS LIST 
       [0072]      11 ,  41  First plate 
         [0073]      15 ,  44  First recessed part 
         [0074]      16 ,  53  Second recessed part 
         [0075]      17  Third recessed part 
         [0076]      21  Second plate 
         [0077]      24  Fourth recessed part 
         [0078]      25  Fifth recessed part 
         [0079]      26  Sixth recessed part 
         [0080]      27 ′,  28 ′,  45 ′ Groove 
         [0081]      27 ,  28 ,  45  Flow path 
         [0082]      31  Fluid introducing port 
         [0083]      33  Glass tube introducing port 
         [0084]      34  Connection part 
         [0085]      91  Tube connecter