Abstract:
The invention discloses a cell culture and experiment device used in the field of biological and genetic engineering experiment apparatus, comprising a central distribution compartment, a culture compartment, a treatment compartment, and pipelines for delivering liquid between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment. The central distribution compartment is equipped with a distribution chamber and a piston which can be moved forward and backward in the distribution chamber to alter the working volume of the distribution chamber. At the bottom of the distribution chamber, the central distribution compartment is equipped with a distribution valve controlling the connectivity between the distribution chamber and any of the channels. The invention provides a miniaturized apparatus integrating the central distribution compartment, the culture compartment and the treatment compartment, which can replace manual operations, save time and labor, and avoid wasting experimental raw material.

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
       [0001]    This application is a continuation application of a PCT application No. PCT/CN2014/075210, filed on Apr. 11, 2014, which claims priority to Chinese Patent Application No. 201410112722.6, filed on Mar. 25, 2014, the entire contents of which are incorporated herein by reference. 
     
    
     TECHNICAL FIELD 
       [0002]    The disclosure is applied to the field of biological and genetic engineering experiment devices, and particularly relates to a cell culture and experiment device. 
       BACKGROUND 
       [0003]    In the field of microbiology, particularly the fields of biological engineering, genetic engineering and the like, researchers execute experiment operations using cultured cells so as to verify their theories and experiments. These basic experiment operations include, but are not limited to, 
         [0004]    1, culturing cells, particularly reproducing the cells in a liquid culture medium determined by one or more components; 
         [0005]    2, measuring cell density; 
         [0006]    3, separating the cells from the liquid culture medium; 
         [0007]    4, re-suspending the cells using fresh liquid; 
         [0008]    5, operating the cells by means of a chemical way, an electric way or other physical ways, for example, introducing genetic materials such as plasmids or oligonucleotides; 
         [0009]    6, sterilizing an instrument using alcohol or other solutions; and 
         [0010]    7, cleaning the instrument using water. 
         [0011]    A current mainstream experiment flow refers to sequentially completing the above steps in small batches by means of manual operations. Commonly used traditional experiment instruments include: a test tube, a shake flask, a shaker, a culture dish, a cuvette, an injector, a pipettor, a centrifugal machine, a filter membrane and the like. When multiple turns of experiment operations or experiment operations on a plurality of samples are executed, it is necessary to consume a great amount of time and labour. 
         [0012]    Although a certain degree of automation can be obtained by combining traditional instruments into a mini plant and adding a control assembly, the solution has the defects that each traditional instrument is not designed for combination, mutual specifications do not match, the combined mini plant is too large for a traditional biochemical laboratory, and a relatively large amount of needed cell culture fluid will make raw experimental materials too expensive. 
       SUMMARY 
       [0013]    To solve the above problems, the disclosure provides a small cell culture and experiment device which takes the place of manual operation, is capable of completing various experimental projects, saves time and labour and avoids waste of raw experimental materials. 
         [0014]    To solve the technical problem, the disclosure adopts the technical solution as follows. A cell culture and experiment device may include a central distribution compartment, a culture compartment, a treatment compartment, and pipelines for delivering liquid between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment, wherein a distribution chamber and a piston capable of moving back and forth in the distribution chamber to change the working volume of the distribution chamber are provided in the central distribution compartment, and a distribution valve for controlling the distribution chamber to be communicated with any pipeline is arranged at the bottom end of the distribution chamber in the central distribution compartment. 
         [0015]    Furthermore, as an improvement of the technical solution of the disclosure, the central distribution compartment, the culture compartment and the treatment compartment may be arranged separately, the central distribution compartment may surround the distribution valve to form a plurality of mounting surfaces which can be connected to the culture compartment or the treatment compartment, a central pipeline leading from the distribution valve to each mounting surface may be arranged on the central distribution compartment, the distribution valve may include a central cylinder hole provided at the bottom end of the distribution chamber and a central valve element which is inserted into the central cylinder hole and can rotate in the central cylinder hole, a central flow channel may be provided on the central valve element, and when the central valve element rotates, the central flow channel may communicate the distribution chamber with any central pipeline. 
         [0016]    Furthermore, as an improvement of the technical solution of the disclosure, the culture compartment may include a culture chamber formed by a cylindrical outer wall and a plug arranged at the front end of the outer wall, and a multi-way valve arranged at the rear end of the outer wall. An air hole may be provided on the plug. The multi-way valve may include a first standard shape block which can be connected to the mounting surfaces and is provided with a cylinder hole and a pipeline, and a first valve element which is inserted into the cylinder hole and can rotate in the cylinder hole. A first connector may be arranged on the first standard shape block. A first flow channel may be provided on the first valve element. When the first valve element rotates, the first flow channel may communicate the first connector with the culture chamber by means of the pipeline or communicate the first connector with the distribution valve or communicate the culture chamber with the distribution valve. 
         [0017]    Furthermore, as an improvement of the technical solution of the disclosure, the outer side of the outer wall may be sheathed by a sleeve, a cavity may be formed between the sleeve and the outer wall, an outlet and an inlet communicated with the cavity may be formed at the front end and rear end of the sleeve, and a spiral partition wall may be arranged in the cavity so as to form a channel which surrounds the outer wall and is connected to the outlet and the inlet. 
         [0018]    Furthermore, as an improvement of the technical solution of the disclosure, a spiral guide pipe may surround the outer wall, and the inner diameter of the spiral guide pipe may be smaller than the outer diameter of the culture chamber. 
         [0019]    Furthermore, as an improvement of the technical solution of the disclosure, the treatment compartment may include an electric treatment compartment, the electric treatment compartment may include a second standard shape block which is provided with a pipeline and can be connected to the mounting surfaces, two electrodes may face two sides of the pipeline in the middle of the second standard shape block, electric connectors which can be connected to external power supplies or measurers may be arranged at the outer ends of the two electrodes, an insulating partition sheet may be arranged between the two electrodes in the pipeline, and the insulating partition sheet may form a protrusion controlling liquid to flow through the pipeline. 
         [0020]    Furthermore, as an improvement of the technical solution of the disclosure, the treatment compartment may include a first filter compartment, the first filter compartment may include a third standard shape block which is provided with a pipeline and can be connected to the mounting surfaces, a filter device which divides the pipeline into a front section and a rear section may be arranged in the third standard shape block, the filter device may include a filter membrane and a porous member arranged at the rear side of the filter membrane, the third standard shape block may include a front half part and a rear half part which can be assembled into a whole, an inner chamber for accommodating the filter device may be formed between the front half part and the rear half part, a first spiral guide groove may be formed in the end surface, tightly attached to the filter membrane, of the front half part, and a first port for injecting external liquid may be formed for the first guide groove on the side surface of the front half part. 
         [0021]    Furthermore, as an improvement of the technical solution of the disclosure, the treatment compartment may include a second filter compartment, the second filter compartment may include a fourth standard shape block which is provided with a pipeline and can be connected to the mounting surfaces, an inner filter chamber communicated with a pipeline may be formed inside the fourth standard shape block, an end cap which is hermetically connected to the fourth standard shape block and is internally provided with a pipeline may be arranged at the tail end of the inner filter chamber, a fibre filter membrane extending into the inner filter chamber may be arranged at the inner end of the end cap, a second port communicated with the inner filter chamber may be provided on the side wall of the fourth standard shape block, and the second port may lead into the inner filter chamber along a tangential direction. 
         [0022]    Furthermore, as an improvement of the technical solution of the disclosure, a second spiral guide groove may be provided on the inner wall of the inner filter chamber, and the second guide groove and the second port may be connected and may surround the fibre filter membrane. 
         [0023]    Furthermore, as an improvement of the technical solution of the disclosure, the treatment compartment may include a cell density measurement compartment, the cell density measurement compartment may include a fifth standard shape block which is provided with a pipeline and can be connected to the mounting surfaces, an optical channel transversely penetrating through the pipeline may be provided on the fifth standard shape block, a light source and a light sensor may be arranged at two ends of the optical channel respectively, and transparent waveguide elements may be arranged on two sides of the pipeline between the light source and the light sensor. 
         [0024]    Furthermore, as an improvement of the technical solution of the disclosure, an optical channel transversely penetrating through the distribution chamber may be provided on the central distribution compartment, a light source and a light sensor may be arranged at two ends of the optical channel respectively, and transparent waveguide elements may be arranged on two sides of the distribution chamber between the light source and the light sensor. 
         [0025]    The disclosure has the beneficial effects as follows. The cell culture and experiment device includes at least one culture compartment, a central distribution compartment including a piston and a distribution valve, at least one treatment compartment, and a series of pipelines for delivering liquid between the compartments. 
         [0026]    When in use, cells grow and reproduce in the culture compartment firstly. A cell suspension can be delivered to the treatment compartment from the culture compartment by selecting a distribution valve passage and moving the piston in the distribution chamber. Treatment operations including optical density measurement, cell and culture solution separation, conductivity measurement, electric transformation, temperature rise, temperature reduction and electromagnetic radiation are further completed in the treatment compartment. 
         [0027]    By means of the above design, the disclosure provides a small device integrating a central distribution compartment, a culture compartment and a treatment compartment, thereby taking the place of manual operation while completing cell culture and various experimental projects, saving time and labour, avoiding waste of raw experimental materials, and reducing an opportunity of exposing experimenters under harmful substances. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0028]    The disclosure is further illustrated below in conjunction with the drawings. 
           [0029]      FIG. 1  is a basic structure diagram of an embodiment of the disclosure; 
           [0030]      FIG. 2  is a structural diagram of a distribution valve in an embodiment of the disclosure; 
           [0031]      FIG. 3  is a structural diagram illustrating that a valve element rotates to a position I in an embodiment of the disclosure; 
           [0032]      FIG. 4  is a structural diagram illustrating that a valve element rotates to a position II in an embodiment of the disclosure; 
           [0033]      FIG. 5  is a structural diagram illustrating that a valve element rotates to a position III in an embodiment of the disclosure; 
           [0034]      FIG. 6  is a structural diagram illustrating that a valve element rotates to a position III′ in an embodiment of the disclosure; 
           [0035]      FIG. 7  is a structural diagram illustrating that a valve element rotates to a position IV in an embodiment of the disclosure; 
           [0036]      FIG. 8  is a section view of a part A-A in  FIG. 1 ; 
           [0037]      FIG. 9  is a section view of a part B-B in  FIG. 8 ; 
           [0038]      FIG. 10  is a structural diagram of an outer wall surrounded by a spiral guide pipe in an embodiment of the disclosure; 
           [0039]      FIG. 11  is a structural diagram of an electric treatment compartment in an embodiment of the disclosure; 
           [0040]      FIG. 12  is a section view of a part C-C in  FIG. 11 ; 
           [0041]      FIG. 13  is a structural diagram of a first filter compartment in an embodiment of the disclosure; 
           [0042]      FIG. 14  is a section view of a part D-D in  FIG. 13 ; 
           [0043]      FIG. 15  is an explosive structural diagram of a filter compartment in an embodiment of the disclosure; 
           [0044]      FIG. 16  is a structural diagram of a first embodiment for a second filter compartment in the disclosure; 
           [0045]      FIG. 17  is a section view of a part E-E in  FIG. 16 ; 
           [0046]      FIG. 18  is a structural diagram of a second embodiment for a second filter compartment in the disclosure; 
           [0047]      FIG. 19  is a structural diagram of a first embodiment for a cell density measurement compartment in the disclosure; 
           [0048]      FIG. 20  is a structural diagram of a second embodiment for a cell density measurement compartment in the disclosure; 
           [0049]      FIG. 21  is a diagram of an idea of adopting a general shape block as a standard shape block in the disclosure; and 
           [0050]      FIG. 22  is a structural diagram of a composite treatment compartment in the disclosure. 
       
    
    
     DETAILED DESCRIPTION OF THE EMBODIMENTS 
       [0051]    With reference to  FIG. 1  to  FIG. 22 , the disclosure provides a cell culture and experiment device, which includes a central distribution compartment  1 , a culture compartment  2 , a treatment compartment  3 , and pipelines  4  for delivering liquid between the central distribution compartment  1  and the culture compartment  2  and between the central distribution compartment  1  and the treatment compartment  3 , wherein a distribution chamber  11  and a piston  12  capable of moving back and forth in the distribution chamber  11  to change the working volume of the distribution chamber  11  are provided in the central distribution compartment  1 , and a distribution valve  13  for controlling the distribution chamber  11  to be communicated with any pipeline  4  is arranged at the bottom end of the distribution chamber  11  in the central distribution compartment  1 . 
         [0052]    With reference to  FIG. 1 , the central distribution compartment  1 , the culture compartment  2  and the treatment compartment  3  are arranged separately, the central distribution compartment  1  surrounds the distribution valve  13  to form a plurality of mounting surfaces which can be connected to the culture compartment  2  or the treatment compartment  3 , three central pipelines  14  leading from the distribution valve  13  to three mounting surfaces are arranged on the central distribution compartment  1 , the distribution valve  13  includes a central cylinder hole  131  provided at the bottom end of the distribution chamber  11  and a central valve element  132  which is inserted into the central cylinder hole  131  and can rotate in the central cylinder hole  131 , a central flow channel  133  is provided on the central valve element  132 , and when the central valve element  132  rotates, the central flow channel  133  can communicate the distribution chamber  11  with any central pipeline  14 . 
         [0053]    With reference to  FIG. 2 , a protrusion  134  is arranged at one end of the central valve element  132  of the distribution valve  13 , and a fixed element  135  (for example, a Seeger clamping ring or a coil spring) is arranged at the other end of the central valve element  132  and configured to fix the central valve element  132  to a specific axial direction of the central cylinder hole  131 . Obviously, the fixed element  135  may take the place of the protrusion  134  for fixing the axial direction. 
         [0054]    The central flow channel  133  on the central valve element  132  may be of different shapes. For example, the central flow channel  133  may pass through the central valve element  132  so as to be connected to the central pipelines  14 , two ends facing the central pipelines  14 . The central flow channel  133  is located on the periphery of the central valve element  132  and is configured to be connected to the adjacent central pipelines  14 . In the present embodiment, an angle between the adjacent central pipelines  14  is 90 degrees. By adjusting the length of the central flow channel  133 , the central flow channel  133  may adapt to an included angle of smaller than or greater than 90 degrees. 
         [0055]    Two first elastic sealing elements  136  are arranged on two sides of the central flow channel  133  on the central valve element  132  and are configured to prevent liquid from leaking along the long axis of the central valve element  132 . 
         [0056]      FIGS. 3-7  illustrate four working positions of the distribution valve  13 . When the central valve element  132  rotates to a position I, the culture compartment  2  is connected to the distribution chamber  11 . The treatment compartment  3  is communicated with the lower central pipeline  14 , and liquid can be delivered by means of an external pump. 
         [0057]    When the central valve element  132  rotates to a position II, the distribution chamber  11  is connected to the treatment compartment  3 , and the upper and lower central pipelines  14  are closed. 
         [0058]    When the central valve element  132  rotates to a position III, the culture compartment  2  is connected to the treatment compartment  3 , and the distribution chamber  11  is connected to the lower central pipeline  14 . It is important to note that when the central valve element  132  is at the position III, if it is not desired that the culture compartment  2  is connected to the treatment compartment  3 , the position III may be switched to a position III′. The distribution chamber  11  is different from the central pipelines  14  in diameter, the distribution chamber  11  may be connected to the lower central pipeline  14 , and meanwhile, the culture compartment  2  and the treatment compartment  3  keep closed. 
         [0059]    When the central valve element  132  rotates to a position IV, the central pipelines  14  and the distribution chamber  11  are completely separated from each other, which may be applied to a standby mode of the device. 
         [0060]    Obviously, other valves with different characteristics may also be obtained by means of other combinations of the central pipelines  14 . 
         [0061]    In the disclosure, the piston  12  is inserted into the distribution chamber  11  in the central distribution compartment  1  and can move back and forth, and the piston  12  is connected to a linear drive device (omitted in the drawings) by means of a rigid part  121  and is connected with an elastic part  122 . The elastic part  122  is attached to the front end of the rigid part  121  and can move along an axial direction of the distribution chamber  11 . The design is commonly used for an injector and an injection pump. When the design is used for the device of the disclosure, three advantages are provided as follows. 
         [0062]    (i) While the piston  12  moves along an inner wall of the distribution chamber  11 , the inner wall can be cleaned. The self-cleaning characteristic eliminates additional cleaning steps, such that the same central distribution compartment  1  can be used during treatment of various kinds of liquid. 
         [0063]    (ii) Liquid, suspensions containing cells, gas and the like can be absorbed or pushed. 
         [0064]    (iii) The linear movement of the piston  12  can be easily converted into volume, flow and the like by utilizing the cross section area of the distribution chamber  11 . 
         [0065]    In addition, the distribution valve  13  is directly connected to the distribution chamber  11 , and when different steps are carried out, the quantity of residual liquid between the distribution chamber  11  and the distribution valve  13  is minimized. 
         [0066]    With reference to  FIG. 8  and  FIG. 9 , the culture compartment  2  includes a culture chamber  23  formed by a cylindrical outer wall  21  and a plug  22  arranged at the front end of the outer wall  21 , and a multi-way valve  24  arranged at the rear end of the outer wall  21 . An air hole  221  is provided on the plug  22 . The plug  22  seals the outer wall  21  using a second elastic sealing element  222 . The multi-way valve  24  is similar to the distribution valve  13  in design, and includes a first standard shape block  241  which can be connected to the mounting surfaces and is provided with a cylinder hole and a pipeline  4 , and a first valve element  242  which is inserted into the cylinder hole and can rotate in the cylinder hole. A first connector  243  is arranged on the first standard shape block  241 . A first flow channel  244  is provided on the first valve element  242 . When the first valve element  242  rotates, the first flow channel  244  can isolate the culture chamber  23 , or communicate the culture chamber  23  with the distribution valve  13  or connect the culture chamber  23  to the first connector  243  by means of the pipeline  4 . If air is charged into the culture chamber  23  from the first connector  243 , generated bubbles will supply oxygen to cells in the culture chamber  23 , and meanwhile, a culture solution is stirred and mixed uniformly. 
         [0067]    In order to control the metabolism and growth rate of cells, the outer side of the outer wall  21  is sheathed by a sleeve  25 , a cavity  26  is formed between the sleeve  25  and the outer wall  21 , and an outlet  251  and an inlet  252  communicated with the cavity  26  are formed at the front end and rear end of the sleeve  25 . Cooled or heated liquid can be charged into the formed cavity  26 , and the liquid enters or exits from the cavity  26  through the outlet  251  and the inlet  252 . In order to improve heat conduction, a spiral partition wall can be arranged in the cavity  26  so as to form a channel which surrounds the outer wall  21  and is connected to the outlet  251  and the inlet  252  so as to guide the liquid to flow (omitted in the drawings). 
         [0068]    Another solution of heating or cooling the culture chamber  23  is shown in  FIG. 10 . A spiral guide pipe  27  surrounds the outer wall  21 , and the heated or cooled liquid flows inside the spiral guide pipe  27 . The inner diameter of the spiral guide pipe  27  is slightly smaller than the outer diameter of the culture chamber  23 , so the spiral guide pipe  27  will be tightly attached to the outer wall  21 . When the spiral guide pipe  27  needs to be removed, the spiral guide pipe  27  needs to be slightly loosened so as to increase the inner diameter thereof. 
         [0069]    The treatment compartment  3  provided in the disclosure includes an electric treatment compartment, a filter compartment and a cell density measurement compartment, wherein with reference to  FIG. 11  and  FIG. 12 , the electric treatment compartment includes a second standard shape block  311  which is provided with a pipeline  4  and can be connected to the mounting surfaces, two electrodes  312  face two sides of the pipeline  4  in the middle of the second standard shape block  311 , and electric connectors  313  which can be connected to external power supplies are arranged at the outer ends of the two electrodes  312 . Specifically, the second standard shape block  311  is made from an electric insulating material, and a cell suspension can be treated under an AC, a DC or a transient current or voltage. This may be used for measuring electric properties of the cell suspension or used for transitorily changing the characteristics of cells. For example, a transient high-voltage pulse may deliver macromolecules such as plasmids or oligonucleotides into the cells (electric transformation). When in use, the power supplies are connected to the electrodes  312  by means of the electric connectors  313 , the cell suspension with a certain small volume will be located in an electric field between the two electrodes  312 , and is continuously and electrically shocked. An electric shock frequency matches a flow speed of the cell suspension. In order to avoid accidental contact between the two electrodes  312 , an insulating partition sheet  314  is arranged between the two electrodes  312  in the pipeline  4 , and the insulating partition sheet  314  forms a protrusion controlling the liquid to flow through the pipeline  4 . 
         [0070]    The filter compartment provided in the disclosure is designed to include a first filter compartment and a second filter compartment. Specifically, with reference to  FIG. 13 ,  FIG. 14  and  FIG. 15 , the first filter compartment includes a third standard shape block  321  which is provided with a pipeline  4  and can be connected to the mounting surfaces, a filter device which divides the pipeline  4  into a front section and a rear section is arranged in the third standard shape block  321 , the filter device includes a filter membrane  322  and a porous member  323  arranged at the rear side of the filter membrane  322 , the third standard shape block  321  includes a front half part  324  and a rear half part  325  which can be assembled into a whole, an inner chamber for accommodating the filter device is formed between the front half part  324  and the rear half part  325 , a first spiral guide groove  326  is formed in the end surface, tightly attached to the filter membrane  322 , of the front half part  324 , and a first port  327  for injecting external liquid is formed for the first guide groove  326  on the side surface of the front half part  324 . When in use, the cell suspension flows through the filter membrane  322  and the porous member  323  by means of the pipeline  4 , so as to deposit the cells on a residual liquid surface of the filter membrane  322 . When the filter membrane  322  is replaced, the filter membrane  322  can be replaced by splitting the front half part  324  and the rear half part  325 . After filtration, re-suspension of the cells may be implemented by means of two methods as follows. 
         [0071]    (i) Fresh liquid may reversely press the filter membrane  322  from the reverse side of the filter membrane  322 . 
         [0072]    (ii) Fresh liquid may be injected from the first port  327 , and the first spiral guide groove  326  will guide the fresh liquid to flow on the residual liquid surface of the filter membrane  322 . It is important to note that the first port  327  needs to be connected to a valve and keeps closed in a filtration process. In this case, liquid in the cell suspension will penetrate through the filter membrane  322 , and the cells will stay on the residual liquid surface of the filter membrane  322 . 
         [0073]    With reference to  FIG. 16  and  FIG. 17 , the second filter compartment includes a fourth standard shape block  331  which is provided with a pipeline  4  and can be connected to the mounting surfaces, an inner filter chamber  332  communicated with a pipeline  4  is formed inside the fourth standard shape block  331 , an end cap  333  which is hermetically connected to the fourth standard shape block  331  and is internally provided with a pipeline  4  is arranged at the tail end of the inner filter chamber  332 , a fibre filter membrane  334  extending into the inner filter chamber  332  is arranged at the inner end of the end cap  333 , a second port  335  communicated with the inner filter chamber  332  is provided on the side wall of the fourth standard shape block  331 , and the second port  335  leads into the inner filter chamber  332  along a tangential direction. The inner end of the end cap  333  forms a cylindrical protrusion. The cylindrical protrusion is sheathed by the fibre filter membrane  334 , and the fibre filter membrane  334  is fixed and sealed by resin. The end cap  333  integrally seals the tail end of the inner filter chamber  332  by means of an elastic sealing element. The cell suspension enters the inner filter chamber  332 , and flows along the outer side of the fibre filter membrane  334 . By designing the outer diameter of the inner filter chamber  332  to be slightly larger than that of the fibre filter membrane  334 , spacing between the inner filter chamber  332  and the fibre filter membrane  334  can be controlled to be very small. While the cells are deposited on the outer side of the fibre filter membrane  334 , the liquid will penetrate through the fibre filter membrane  334  and flow out from the pipeline  4  of the end cap  333 . For the second filter compartment, there are two methods for re-suspending the cells on the outer side of the fibre filter membrane  334 . (1) Fresh liquid is injected from the end cap  333  and reversely presses the fibre filter membrane  334 . (2) Fresh liquid enters the inner filter chamber  332  from the second port  335  along the tangential direction so as to generate circulation facilitating cell re-suspension. 
         [0074]    With reference to  FIG. 18 , as a further improvement, in the second filter compartment, a second spiral guide groove  336  is provided on the inner wall of the inner filter chamber  332 , and the second guide groove  336  and the second port  335  are connected and surround the fibre filter membrane  334 . When the cells are re-suspended, fresh liquid will flow along the second spiral guide groove  336  and re-suspend the cells. Similarly, by controlling the inner diameter of the second spiral guide groove  336 , the whole second filter compartment may be kept in small volume. 
         [0075]    With reference to  FIG. 19 , the cell density measurement compartment includes a fifth standard shape block  341  which is provided with a pipeline  4  and can be connected to the mounting surfaces, an optical channel transversely penetrating through the pipeline  4  is provided on the fifth standard shape block  341 , a light source  342  and a light sensor  343  are arranged at two ends of the optical channel respectively, and transparent waveguide elements  344  are arranged on two sides of the pipeline  4  between the light source  342  and the light sensor  343 . Specifically, light emitted by the light source  342  (which may be a light emitting diode) passes through one transparent waveguide element  344 , interacts with the cell suspension, is received by the other transparent waveguide element  344 , and then reaches the light sensor  343  (phototransistor). The cell density measurement of the cell suspension is further completed. 
         [0076]    As shown in  FIG. 20 , the cell density measurement compartment and the central distribution compartment  1  are arranged integrally, that is, an optical channel transversely penetrating through the distribution chamber  11  is provided on the central distribution compartment  1 , such that a light source  342  and a light sensor  343  are located at positions, on two sides of the distribution chamber  11 , in the optical channel, and transparent waveguide elements are arranged on two sides of the distribution chamber  11  between the light source  342  and the light sensor  343 . The design reduces liquid amount needed during cell density measurement, and reduces needed mechanical motions. Once connection is established between the culture compartment  2  and the central distribution compartment  1 , after the cells reach the distribution chamber  11 , cell density can be directly measured. Besides, because the distribution chamber  11  has a larger diameter, the quantity of the measured cells is larger, and a measurement result is more accurate. 
         [0077]    To enable an experimenter to freely select a compartment to mount the device according to an experiment flow, a standard shape block having a general shape  5  is adopted in the disclosure as a basis to be applied to a culture compartment  2 , a central distribution compartment  1  and all treatment compartments  3 . Imprints of the culture compartment  2 , the central distribution compartment  1  and all the treatment compartments  3  can be regarded as multiples of the general shape  5 , and therefore the culture compartment  2 , the central distribution compartment  1  and all the treatment compartments  3  are easily combined into different configurations.  FIG. 21  shows a design idea, the general shape  5  is represented by a square. The device shown in  FIG. 21  includes two culture compartments  2 , a central distribution compartment  1 , a treatment compartment  3  and three valves. Imprints of the central distribution compartment  1  can be regarded as three general shapes  5 . Besides, the imprints of all compartments are equal to a general shape. 
         [0078]      FIG. 22  shows a composite treatment compartment. An electric treatment compartment and a first filter compartment described above are combined in a standard shape block. The combination has the advantages that the suspension volume of a pipeline  4  is reduced to the greatest extent, and the combination matches the design of the general shape  5 . 
         [0079]    With reference to  FIG. 1 , in order to connect two adjacent compartments, external outlets of pipelines  4  of different compartments will be aligned along with self-border alignment of the compartments. Grooves  41  are provided around the external outlets of the pipelines  4  of different compartments, and sealing elements are arranged in the grooves  41  and are configured to seal the pipelines  4  between the compartments so as to prevent liquid from leaking at a joint. 
         [0080]    In order to guarantee that two adjacent compartments are completely aligned, a matching hole  43  is provided on each compartment, and can realize perfect connection by means of a simple connector element  44 , wherein the connector element  44  may adopt a simple cylindrical pin or flat key. 
         [0081]    Certainly, the creation of the disclosure is not limited to the above implementation, those skilled in the art can also make equivalent deformations or replacements without departing from the spirit of the disclosure, and these equivalent deformations or replacements fall within the scope limited by the claims of the present application.