Abstract:
The invention discloses application of an enrofloxacin salt in preparation of an oral preparation for pigs. The enrofloxacin salt is at least one of salts formed by enrofloxacin and metal ions, and the metal ions are zinc, copper, calcium, magnesium, iron, cobalt, manganese, chromium, silver or nickel. The enrofloxacin salt formed by the enrofloxacin and the metal ions can significantly improve the palatability of the enrofloxacin to pigs; furthermore pharmacokinetics and artificial infection prove that the enrofloxacin salt formed by the enrofloxacin and the metal ions does not affect bioavailability, so enrofloxacin salt can be developed into the oral preparation administrated via the digestive tract, for pig-raising without affecting normal feed intake of the pigs.

Description:
FIELD OF THE INVENTION 
       [0001]    The invention belongs to the field of livestock and poultry breeding and specifically relates to application of an enrofloxacin salt in preparation of an oral preparation for pigs. 
       BACKGROUND OF THE INVENTION 
       [0002]    Enrofloxacin is the first special quinolone type antibiotic species for animals, has a broad-spectrum bactericidal effect and is effective to bacteria in a stationary phase and a growth phase. The enrofloxacin has a good antibacterial effect on a variety of gram-negative bacilli and cocci, and the MIC against the overwhelming majority of strains of sensitive bacteria is lower than 1 ug/ml. The enrofloxacin is widely applied in the aspect of treatment of animal bacterial infections. At present, the forms of the enrofloxacin in commercial products include enrofloxacin base, enrofloxacin sodium and enrofloxacin hydrochloride. It is found in the production practices that the enrofloxacin salt in the three forms has very poor palatability to the pigs and can not be orally administered or administered by mixing, possibly due to the special physiological basis of the pigs. Thus, the enrofloxacin has long been only limited to injections in the application in pig-raising industry. 
       SUMMARY OF THE INVENTION 
       [0003]    An object of the invention is providing application of an enrofloxacin salt in preparation of an oral preparation for pigs. 
         [0004]    In the testing process, the inventors found that salts formed by enrofloxacin and part of monovalent (Ag +  etc.) or divalent (Zn 2+  or Cu 3+  etc.) metal ions could significantly improve the palatability of the enrofloxacin, and could be developed into a preparation administered via the digestive tract, for pig-raising production without affecting the normal feed intake of the pigs. In addition, pharmacokinetics and artificial infection test prove that the salt formed by the enrofloxacin and the metal ions does not affect bioavailability. 
         [0005]    Thus, the invention provides application of an enrofloxacin salt in preparation of an oral preparation for pigs, wherein the enrofloxacin salt is at least one of salts formed by enrofloxacin and metal ions, and the metal ions are zinc, copper, calcium, magnesium, iron, cobalt, manganese, chromium, silver or nickel. 
         [0006]    The enrofloxacin salt is further preferably enrofloxacin zinc, enrofloxacin copper or enrofloxacin silver. 
         [0007]    Preferably, the enrofloxacin zinc is a complex formed by the enrofloxacin and zinc ions according to the molar ratio of 2:1, the enrofloxacin copper is a complex formed by the enrofloxacin and copper ions respectively according to the molar ratio of 2:1, and the enrofloxacin silver is a salt formed by the enrofloxacin and silver ions according to the molar ratio of 1:1. 
         [0008]    The oral preparation includes the oral preparation which is orally given or administered via feed or drinking water, and including, e.g., powder, granules, suspensions, emulsions, tablets and pills, etc. 
         [0009]    The invention also provides an oral preparation for pigs containing an enrofloxacin salt as an active ingredient, wherein the enrofloxacin salt is at least one of salts formed by enrofloxacin and metal ions, and the metal ions are zinc, copper, calcium, magnesium, iron, cobalt, manganese, chromium, silver or nickel. 
         [0010]    The enrofloxacin salt is further preferably enrofloxacin zinc, enrofloxacin copper or enrofloxacin silver. 
         [0011]    Preferably, the enrofloxacin zinc is a complex formed by the enrofloxacin and zinc ions according to the molar ratio of 2:1, the enrofloxacin copper is a complex formed by the enrofloxacin and copper ions respectively according to the molar ratio of 2:1, and the enrofloxacin silver is a salt formed by the enrofloxacin and silver ions according to the molar ratio of 1:1. 
         [0012]    The oral preparation includes the oral preparation which is orally given or administered via feed or drinking water, and including, e.g., powder, granules, suspensions, emulsions, tablets and pills, etc. 
         [0013]    The enrofloxacin salt formed by the enrofloxacin and the metal ions in the invention can significantly improve the palatability of the enrofloxacin to pigs. Furthermore, pharmacokinetics study and artificial infection test prove that the enrofloxacin salt formed by the enrofloxacin and the metal ions does not affect bioavailability. As a result, the enrofloxacin salt can be developed into an oral preparation administrated via the digestive tract, for pig-raising without affecting normal feed intake of the pigs. 
     
    
     DETAILED DESCRIPTION OF THE EMBODIMENTS 
       [0014]    The following embodiments are used for further describing the invention rather than limiting the invention. 
       Embodiment 1 
     Preparation of Enrofloxacin Salt 
     1. Preparation of Enrofloxacin Zinc 
       [0015]    The general formula for the preparation of the enrofloxacin zinc is as follows. 100.0 g of enrofloxacin sodium was dissolved in water and the volume was set to 1000 mL to obtain an enrofloxacin sodium solution. 40.0 g of zinc sulfate heptahydrate was dissolved in water and the volume was set to 1000 ml to obtain a zinc sulfate solution. The zinc sulfate solution was slowly added dropwise into the enrofloxacin sodium solution at room-temperature under stirring for reaction, and the stirring for reaction was continuously performed for 1 h after the end of adding to complete reaction. A suction filtration was performed on the reaction solution, a suction filtration and washing were performed on the filter cake with 3000 ml of water, and the filtration residues were dried at 80° C. till constant weight so as to obtain the enrofloxacin zinc. 99.30 g of sample was produced, and the reaction yield was 96.9%. 
         [0000]      2C 19 H 21 FN 3 O 3 Na+ZnSO 4 .7H 2 O═(C 19 H 21 FN 3 O 3 ) 2 Zn+Na 2 SO 4 +7H 2 O
 
       2. Preparation of Enrofloxacin Copper 
       [0016]    The general formula for the preparation of the enrofloxacin copper is as follows. 100.0 g of enrofloxacin sodium was dissolved in water and the volume was set to 1000 mL to obtain an enrofloxacin sodium solution. 40.0 g of copper sulfate pentahydrate was dissolved in water and the volume was set to 1000 ml to obtain a copper sulfate solution. The copper sulfate solution was slowly added dropwise into the enrofloxacin sodium solution at room-temperature under stirring for reaction, and the stirring for reaction was continuously performed for 1 h after the end of adding to complete reaction. A suction filtration was performed on the reaction solution, a suction filtration and washing were performed on the filter cake with 3000 ml of water, and the filtration residues were dried at 80° C. till constant weight so as to obtain the enrofloxacin copper. 98.2 g of sample was produced, and the reaction yield was 95.93%. 
         [0000]      2C 19 H 21 FN 3 O 3 Na+CuSO 4 .5H 2 O═(C 19 H 21 FN 3 O 3 ) 2 Cu+Na 2 SO 4 +5H 2 O
 
       3. Preparation of Enrofloxacin Silver 
       [0017]    The general formula for the preparation of the enrofloxacin silver is as follows. 100.0 g of enrofloxacin sodium was dissolved in water and the volume was set to 1000 mL to obtain an enrofloxacin sodium solution. 50.0 g of silver nitrate was dissolved in water and the volume was set to 1000 ml to obtain a silver nitrate solution. The silver nitrate solution was slowly added dropwise into the enrofloxacin sodium solution at room-temperature under stirring for reaction, and the stirring for reaction was continuously performed for 1 h after the end of adding to complete reaction. A suction filtration was performed on the reaction solution, a suction filtration and washing were performed on the filter cake with 3000 ml of water, and filtration residues were dried at 80° C. till constant weight so as to obtain the enrofloxacin silver. 126.6 g of sample was produced, and the reaction yield was 96.89%. 
         [0000]      C 19 H 21 FN 3 O 3 Na+AgNO 3 ═C 19 H 21 FN 3 O 3 Ag+NaNO 3  
 
       Embodiment 2 
     Influence of Enrofloxacin Base, Sodium Salt and Hydrochloride on Palatability of Pig Feed 
       [0018]    100 105-day-old healthy duroc-landrace-yorkshire hybrid pigs with similar body weights were divided into 10 test groups as shown in Table 1, and each group comprised 10 pigs. Enrofloxacin base, enrofloxacin sodium and enrofloxacin hydrochloride were respectively added in feed in each group. All the test pigs were synchronously fasted for 12 h before testing. The test pigs in each group were respectively subjected to free feed intake of feed containing enrofloxacin in different forms after the 12 h fast. Observation was performed for 30-60 minutes till the test pigs did not take feed any more. Feed consumption of different test groups was subjected to statistics and the influence of different forms of the enrofloxacin added in the feed on the feed intake of the pigs was evaluated. The pure feed which was not added with the enrofloxacin or the salt thereof was taken as control. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Test grouping of influence of enrofloxacin base, enrofloxacin 
               
               
                 sodium or enrofloxacin hydrochloride on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose* (ppm) 
                 Number of test pigs 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin base 
                 5 
                 10 
               
               
                 2 
                 enrofloxacin base 
                 25 
                 10 
               
               
                 3 
                 enrofloxacin base 
                 100 
                 10 
               
               
                 4 
                 enrofloxacin sodium 
                 5 
                 10 
               
               
                 5 
                 enrofloxacin sodium 
                 25 
                 10 
               
               
                 6 
                 enrofloxacin sodium 
                 100 
                 10 
               
               
                 7 
                 enrofloxacin 
                 5 
                 10 
               
               
                   
                 hydrochloride 
               
               
                 8 
                 enrofloxacin 
                 25 
                 10 
               
               
                   
                 hydrochloride 
               
               
                 9 
                 enrofloxacin 
                 100 
                 10 
               
               
                   
                 hydrochloride 
               
               
                 10 
                 control group (—) 
                   
                 10 
               
               
                   
               
               
                 *All the test doses were calculated by enrofloxacin. 
               
             
          
         
       
     
         [0019]    As the enrofloxacin base, the enrofloxacin sodium or the enrofloxacin hydrochloride was added in the feed, the feed intake of each test pig was significantly reduced. Comparing with a blank control group, the feed intake in each of the test groups added with 5 ppm of enrofloxacin base, enrofloxacin sodium or enrofloxacin hydrochloride respectively was reduced by 47.2%, 50.8% and 49.5% respectively, and the test pigs in the group added with 100 ppm of the enrofloxacin in different forms showed apastia (See table 2 for the results). 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Test results of influence of enrofloxacin 
               
               
                 and salts thereof on palatability of pig feed 
               
             
          
           
               
                   
                 Using 
                   
                   
                   
                 Decline 
               
               
                   
                 amount 
                 Dose 
                 Total feed 
                 Average feed 
                 in feed 
               
               
                 Group 
                 (ppm) 
                 (ppm) 
                 intake (kg) 
                 intake (kg) 
                 intake (%) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin 
                 5 
                 6.81 
                 0.681 
                 47.2 
               
               
                   
                 base 
               
               
                 2 
                 enrofloxacin 
                 25 
                 3.32 
                 0.332 
                 74.2 
               
               
                   
                 base 
               
               
                 3 
                 enrofloxacin 
                 100 
                 0.86 
                 0.086 
                 93.3 
               
               
                   
                 base 
               
               
                 4 
                 enrofloxacin 
                 5 
                 6.34 
                 0.634 
                 50.8 
               
               
                   
                 sodium 
               
               
                 5 
                 enrofloxacin 
                 25 
                 3.47 
                 0.347 
                 73.1 
               
               
                   
                 sodium 
               
               
                 6 
                 enrofloxacin 
                 100 
                 0.76 
                 0.076 
                 94.1 
               
               
                   
                 sodium 
               
               
                 7 
                 enrofloxacin 
                 5 
                 6.51 
                 0.651 
                 49.5 
               
               
                   
                 hydrochloride 
               
               
                 8 
                 enrofloxacin 
                 25 
                 3.41 
                 0.341 
                 73.4 
               
               
                   
                 hydrochloride 
               
               
                 9 
                 enrofloxacin 
                 100 
                 0.81 
                 0.081 
                 93.7 
               
               
                   
                 hydrochloride 
               
               
                 10 
                 control group 
                 — 
                   
                 1.289 
                 — 
               
               
                   
                 (—) 
               
               
                   
               
             
          
         
       
     
       Embodiment 3 
     Influence of Enrofloxacin Salts on Palatability of Pig Feed 
       [0020]    150 110-day-old healthy duroc-landrace-yorkshire hybrid pigs with similar body weights were divided into 5 test groups as shown in Table 3, and each group performed three repeats. The feed in each group was added with different doses of the enrofloxacin or the salt thereof. All the test pigs were synchronously fasted for 12 h before testing. The test pigs in each group were respectively subjected to free feed intake of feed containing enrofloxacin or the salt thereof after the 12 h fast. Observation was performed for 30-60 minutes till the test pigs did not take feed any more. Feed consumption of different test groups was subjected to statistics and the influence of different forms of the enrofloxacin on the palatability of pig feed was evaluated. The pure feed which was not added with the enrofloxacin or the salt thereof was taken as control. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Test grouping of influence of enrofloxacin 
               
               
                 and salts thereof on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose* (ppm) 
                 Number of test pigs 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin 
                 100 
                 10 × 3 
               
               
                   
                 sodium 
               
               
                 2 
                 enrofloxacin zinc 
                 100 
                 10 × 3 
               
               
                 3 
                 enrofloxacin 
                 100 
                 10 × 3 
               
               
                   
                 copper 
               
               
                 4 
                 enrofloxacin 
                 100 
                 10 × 3 
               
               
                   
                 silver 
               
               
                 5 
                 control group (—) 
                 — 
                 10 × 3 
               
               
                   
               
               
                 *All the test doses were calculated by enrofloxacin. 
               
             
          
         
       
     
         [0021]    As the enrofloxacin or the salt was added into the feed, enrofloxacin sodium basically completely inhibited the feed intake behavior of pigs, while salts formed by enrofloxacin and zinc, copper and silver had no significant influence on the feed intake of the test pigs (See table 4 for the results). 
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Test results of influence of enrofloxacin 
               
               
                 and salts thereof on palatability of pig feed 
               
             
          
           
               
                   
                   
                   
                 Total 
                   
               
               
                   
                   
                 Number of 
                 feed intake 
                 Average feed 
               
               
                 Group 
                 Test sample (ppm) 
                 animals 
                 (kg) 
                 intake (kg) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin sodium 
                 10 × 3 
                 2.18 
                 0.073 ± 0.015 B   
               
               
                   
                 (100 ppm) 
               
               
                 2 
                 enrofloxacin zinc 
                 10 × 3 
                 39.55 
                 1.318 ± 0.182 A   
               
               
                   
                 (100 ppm) 
               
               
                 3 
                 enrofloxacin copper 
                 10 × 3 
                 38.90 
                 1.297 ± 0.203 A   
               
               
                   
                 (100 ppm) 
               
               
                 4 
                 enrofloxacin silver 
                 10 × 3 
                 38.72 
                 1.291 ± 0.180 A   
               
               
                   
                 (100 ppm) 
               
               
                 5 
                 control group (—) 
                 10 × 3 
                 39.01 
                 1.300 ± 0.166 A   
               
               
                   
               
               
                 Note: 
               
               
                 Data in the same column with different capital letters as superscripts showed significant difference (P &lt; 0.05). 
               
             
          
         
       
     
       Embodiment 4 
     Dose Research of Influence of Enrofloxacin Zinc on Palatability of Pig Feed 
       [0022]    120 110-day-old healthy duroc-landrace-yorkshire hybrid pigs with similar body weights were divided into 4 test groups as shown in Table 5, each group performed three repeats, and each repeat was performed on 10 pigs. The feed in each group was added with different doses of the enrofloxacin zinc respectively. All the test pigs were synchronously fasted for 12 h before testing. The test pigs in each group were respectively subjected to free feed intake of feed containing enrofloxacin zinc in different forms after the 12 h fast. Observation was performed for 30-60 minutes till the test pigs did not take feed any more. Feed consumption of different test groups was subjected to statistics and the influence of different doses of the enrofloxacin zinc added into the feed on palatability of pig feed was evaluated. The pure feed which was not added with the enrofloxacin or the salt thereof was taken as control. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Test grouping of dose research of influence 
               
               
                 of enrofloxacin zinc on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose* (ppm) 
                 Number of test pigs 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin zinc 
                 500 
                 10 × 3 
               
               
                 2 
                 enrofloxacin zinc 
                 250 
                 10 × 3 
               
               
                 3 
                 enrofloxacin zinc 
                 100 
                 10 × 3 
               
               
                 4 
                 control group (—) 
                 — 
                 10 × 3 
               
               
                   
               
               
                 *All the test doses were calculated by enrofloxacin. 
               
             
          
         
       
     
         [0023]    As feed was added with different doses of enrofloxacin zinc, the feed intake of the test pigs had no significant difference from that of a control group (the pure feed which was not added with enrofloxacin or the salt thereof) (See table 6 for the results). 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Test results of influence of enrofloxacin 
               
               
                 and salts thereof on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose (ppm) 
                 Average feed intake (kg) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin zinc 
                 500 
                 1.338 ± 0.259 A   
               
               
                 2 
                 enrofloxacin zinc 
                 250 
                 1.319 ± 0.297 A   
               
               
                 3 
                 enrofloxacin zinc 
                 100 
                 1.335 ± 0.342 A   
               
               
                 4 
                 control group (—) 
                 — 
                 1.324 ± 0.315 A   
               
               
                   
               
               
                 Note: 
               
               
                 *Data in the same column with different capital letters as superscripts showed significant difference (P &lt; 0.05). 
               
             
          
         
       
     
       Embodiment 5 
     Dose Research of Influence of Enrofloxacin Copper on Palatability of Pig Feed 
       [0024]    120 115-day-old healthy duroc-landrace-yorkshire hybrid pigs with similar body weights were divided into 4 test groups as shown in Table 7, each group performed three repeats, and each repeat was performed on 10 pigs. The feed in each group was added with different doses of the enrofloxacin copper respectively. All the test pigs were synchronously fasted for 12 h before testing. The test pigs in each group were respectively subjected to free feed intake of feed containing enrofloxacin copper in different forms after the 12 h fast. Observation was performed for 30-60 minutes till the test pigs did not take feed any more. Feed consumption of different test groups was subjected to statistics and the influence of different doses of the enrofloxacin copper added into the feed on palatability of pig feed was evaluated. The pure feed which was not added with the enrofloxacin or the salt thereof was taken as control. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 Test grouping of dose research of influence of 
               
               
                 enrofloxacin copper on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose* (ppm) 
                 Number of test pigs 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin copper 
                 500 
                 10 × 3 
               
               
                 2 
                 enrofloxacin copper 
                 250 
                 10 × 3 
               
               
                 3 
                 enrofloxacin copper 
                 100 
                 10 × 3 
               
               
                 4 
                 control group (—) 
                 — 
                 10 × 3 
               
               
                   
               
               
                 *All the test doses were calculated by enrofloxacin. 
               
             
          
         
       
     
         [0025]    As feed was added with different doses of enrofloxacin copper, the feed intake of the test pigs had no significant difference from that of a control group (See table 8 for the results). 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 Test results of influence of enrofloxacin copper on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose (ppm) 
                 Average feed intake (kg) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin copper 
                 500 
                 1.352 ± 0.351 A   
               
               
                 2 
                 enrofloxacin copper 
                 250 
                 1.369 ± 0.349 A   
               
               
                 3 
                 enrofloxacin copper 
                 100 
                 1.355 ± 0.397 A   
               
               
                 4 
                 control group (—) 
                 — 
                 1.358 ± 0.381 A   
               
               
                   
               
               
                 Note: 
               
               
                 *Data in the same column with different capital letters as superscripts showed significant difference (P &lt; 0.05). 
               
             
          
         
       
     
       Embodiment 6 
     Dose Research of Influence of Enrofloxacin Silver on Palatability of Pig Feed 
       [0026]    120 120-day-old healthy duroc-landrace-yorkshire hybrid pigs with similar body weights were divided into 4 test groups as shown in Table 9, each group performed three repeats, and each repeat was performed on 10 pigs. The feed in each group was added with different doses of the enrofloxacin silver respectively. All the test pigs were synchronously fasted for 12 h before testing. The test pigs in each group were respectively subjected to free feed intake of feed containing enrofloxacin silver in different forms after the 12 h fast, observation was performed for 30-60 minutes till the test pigs did not take feed any more. Feed consumption of different test groups was subjected to statistics and the influence of different doses of the enrofloxacin silver added into the feed on palatability of pig feed was evaluated. The pure feed which was not added with the enrofloxacin or the salt thereof was taken as control. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 Test grouping of dose research of influence of 
               
               
                 enrofloxacin silver on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose* (ppm) 
                 Number of test pigs 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin silver 
                 500 
                 10 × 3 
               
               
                 2 
                 enrofloxacin silver 
                 250 
                 10 × 3 
               
               
                 3 
                 enrofloxacin silver 
                 100 
                 10 × 3 
               
               
                 4 
                 control group (—) 
                 — 
                 10 × 3 
               
               
                   
               
               
                 *All the test doses were calculated by enrofloxacin. 
               
             
          
         
       
     
         [0027]    As feed was added with different doses of enrofloxacin silver, the feed intake of the test pigs had no significant difference from that of a control group (See table 10 for results). 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 Test results of dose research of influence of 
               
               
                 enrofloxacin silver on palatability of pig feed 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose (ppm) 
                 Average feed intake (kg) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin silver 
                 500 
                 1.379 ± 0.388 A   
               
               
                 2 
                 enrofloxacin silver 
                 250 
                 1.389 ± 0.447 A   
               
               
                 3 
                 enrofloxacin silver 
                 100 
                 1.380 ± 0.416 A   
               
               
                 4 
                 control group (—) 
                 — 
                 1.385 ± 0.406 A   
               
               
                   
               
               
                 Note: 
               
               
                 *Data in the same column with different capital letters as superscripts showed significant difference (P &lt; 0.05). 
               
             
          
         
       
     
       Embodiment 7 
     Influence of Long-Term Application of Enrofloxacin Zinc, Enrofloxacin Copper and Enrofloxacin Silver on Feed Intake of Pigs 
       [0028]    120 120-day-old healthy duroc-landrace-yorkshire hybrid pigs with similar body weights were divided into 4 test groups as shown in Table 11, and each group performed three repeats. Feed in each group was respectively added with 100 ppm of enrofloxacin zinc, enrofloxacin copper or enrofloxacin silver. The feed intake of the test pigs for 7 continuous days was subjected to statistics, the average daily feed intake was calculated, and influence of the normal application of the enrofloxacin zinc and the like on the feed intake of the pigs was evaluated. The pure feed which was not added with the enrofloxacin or the salt thereof was taken as control. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 11 
               
             
             
               
                   
               
               
                 Test grouping of influence of enrofloxacin 
               
               
                 zinc and the like on feed intake of test pigs 
               
             
          
           
               
                 Group 
                 Test sample 
                 Dose* (ppm) 
                 Number of test pigs 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin zinc 
                 100 
                 10 × 3 
               
               
                 2 
                 enrofloxacin 
                 100 
                 10 × 3 
               
               
                   
                 copper 
               
               
                 3 
                 enrofloxacin 
                 100 
                 10 × 3 
               
               
                   
                 silver 
               
               
                 4 
                 control group (—) 
                 — 
                 10 × 3 
               
               
                   
               
               
                 *All the test doses were calculated by enrofloxacin. 
               
             
          
         
       
     
         [0029]    Statistical results of the continuous 7 days showed that as feed was added with enrofloxacin zinc and the like, comparing with a control group which was not added with a medicament, the feed intake behavior and the feed intake of the test pigs were normal, and the feed intake had no significant difference statistically (See table 12 for the results). 
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 12 
               
             
             
               
                   
               
               
                 Test results of influence of enrofloxacin 
               
               
                 zinc and the like on feed intake of test pigs 
               
             
          
           
               
                   
                   
                   
                 Total 
                   
               
               
                   
                   
                 Number of 
                 feed intake 
                 Average feed 
               
               
                 Group 
                 Test sample 
                 animals 
                 (kg) 
                 intake (kg) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin zinc 
                 10 × 3 
                 468.3 
                 2.230 ± 0.321 A   
               
               
                 2 
                 enrofloxacin copper 
                 10 × 3 
                 463.5 
                 2.207 ± 0.333 A   
               
               
                 3 
                 enrofloxacin silver 
                 10 × 3 
                 460.1 
                 2.191 ± 0.280 A   
               
               
                 4 
                 control group (—) 
                 10 × 3 
                 458.9 
                 2.185 ± 0.316 A   
               
               
                   
               
               
                 Note 
               
               
                 Data in the same column with different capital letters as superscripts showed significant difference (P &lt; 0.05). 
               
             
          
         
       
     
       Embodiment 8 
     Pharmacokinetic Research of Enrofloxacin Zinc and the Like in Pigs 
       [0030]    20 40-day-old piglets were divided into four groups with five pigs in each group as shown in Table 13, and the pigs were fed with enrofloxacin in different forms by gastric tubes in an amount of 10 mg/kg body weight respectively. Jugular venous blood collection was performed at 30 min, 1 h, 2 h, 4 h, 8 h, 12 h and 24 h after administration. Plasma was separated, the concentration of the enrofloxacin in the plasma in the different test pigs after administration was determined by high-performance liquid chromatography respectively, and whether the difference in the concentration of the enrofloxacin in the plasma in each test group after administration was significant or not and whether the elimination rule of the enrofloxacin in blood was consistent or not was subjected to statistical analysis. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 13 
               
             
             
               
                   
               
               
                 Test grouping of pharmacokinetic research 
               
               
                 of enrofloxacin zinc and the like in pigs 
               
             
          
           
               
                   
                   
                   
                 Number 
                   
               
               
                   
                   
                 Dose* (mg/kg of 
                 of test 
                 Administration 
               
               
                 Group 
                 Test sample 
                 body weight) 
                 pigs 
                 way 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin 
                 10 
                 5 
                 Administered by 
               
               
                   
                 zinc 
                   
                   
                 gastric tube 
               
               
                 2 
                 enrofloxacin 
                 10 
                 5 
                 Administered by 
               
               
                   
                 copper 
                   
                   
                 gastric tube 
               
               
                 3 
                 enrofloxacin 
                 10 
                 5 
                 Administered by 
               
               
                   
                 silver 
                   
                   
                 gastric tube 
               
               
                 4 
                 enrofloxacin 
                 10 
                 5 
                 Administered by 
               
               
                   
                 sodium 
                   
                   
                 gastric tube 
               
               
                   
               
             
          
         
       
     
         [0031]    The concentration and the elimination rule of the enrofloxacin and ciprofloxacin in the plasma of the test pigs in each test group after administration are as shown in Table 14. The results showed that the peak values of the plasma concentrations of all the test groups were similar, and the elimination law was consistent, indicating that the salts formed by the enrofloxacin and metal ions, such as zinc, copper, silver and the like did not affect the absorption and metabolism processes of the enrofloxacin in vivo. 
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                 TABLE 14 
               
             
             
               
                   
               
               
                 Changing law of plasma concentration of enrofloxacin zinc and the like in pigs (unit: 
               
               
                 ug/ml) 
               
             
          
           
               
                 Group 
                 Test sample 
                 30 min 
                 1 h 
                 2 h 
                 4 h 
                 8 h 
                 12 h 
                 24 h 
               
               
                   
               
               
                 1 
                 enrofloxacin zinc 
                 1.432 ± 0.186 A   
                 2.189 ± 0.204 B   
                 5.321 ± 0.541 C   
                 5.358 ± 0.404 D   
                 2.998 ± 0.216 E   
                 1.762 ± 0.205 F   
                 0.311 ± 0.045 G   
               
               
                 2 
                 enrofloxacin copper 
                 1.417 ± 0.169 A   
                 2.121 ± 0.198 B   
                 5.187 ± 0.558 C   
                 5.465 ± 0.529 D   
                 2.986 ± 0.234 E   
                 1.785 ± 0.215 F   
                 0.294 ± 0.044 G   
               
               
                 3 
                 enrofloxacin silver 
                 1.422 ± 0.189 A   
                 2.250 ± 0.256 B   
                 5.425 ± 0.596 C   
                 5.518 ± 0.498 D   
                 2.977 ± 0.231 E   
                 1.779 ± 0.194 F   
                 0.308 ± 0.048 G   
               
               
                 4 
                 enrofloxacin sodium 
                 1.426 ± 0.191 A   
                 2.230 ± 0.251 B   
                 5.338 ± 0.555 C   
                 5.299 ± 0.484 D   
                 2.987 ± 0.266 E   
                 1.781 ± 0.167 F   
                 0.298 ± 0.042 G   
               
               
                   
               
               
                 Note: 
               
               
                 Data in the same column with different capital letters as superscripts showed significant difference (P &lt; 0.05); and the numerical value of the plasma concentration in the table was the total amount of the enrofloxacin and the ciprofloxacin. 
               
             
          
         
       
     
       Embodiment 9 
     Test Results of Enrofloxacin Zinc and the Like Against  Escherichia Coli  Artificial Infection 
       [0032]    60 30-day-old weaned piglets were divided into six groups with 10 pigs in each group as shown in Table 15, feed was respectively added with enrofloxacin in different forms, and free feed intake was performed. The 33-day-old pigs were intramuscularly injected with pathogenic  escherichia coli , the diarrhea and deaths of the pigs were observed, continuous observation was performed for one week, the onset and deaths were subjected to statistics, and the protection effects of the enrofloxacin in different forms against the pathogenic  escherichia coli  in the artificial infection were compared. Pure feed which was not added with the enrofloxacin in any form was taken as a control group, wherein one portion of pigs in the control group were intramuscularly injected with the pathogenic  escherichia coli  and was taken as the control group without administration but with challenge (the fifth group), and the remainder were not intramuscularly injected with the pathogenic  escherichia coli  and was taken as the control group without administration and without challenge (the sixth group). 
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                 TABLE 15 
               
             
             
               
                   
               
               
                 Test grouping of protection effects of enrofloxacin in 
               
               
                 different forms against  escherichia coli  challenge infection 
               
             
          
           
               
                   
                   
                 Dose* 
                 Number of test 
                   
                 Challenge dose 
               
               
                 Group 
                 Test sample 
                 (ppm) 
                 pigs 
                 Challenge strain 
                 (×107 CFU/pig) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin 
                 100 
                 10 
                 
                   escherichia coli 
                 
                 2.0 
               
               
                   
                 zinc 
                   
                   
                 SGD strain 
               
               
                 2 
                 enrofloxacin 
                 100 
                 10 
                 
                   escherichia coli 
                 
                 2.0 
               
               
                   
                 copper 
                   
                   
                 SGD strain 
               
               
                 3 
                 enrofloxacin 
                 100 
                 10 
                 
                   escherichia coli 
                 
                 2.0 
               
               
                   
                 silver 
                   
                   
                 SGD strain 
               
               
                 4 
                 enrofloxacin 
                 100 
                 10 
                 
                   escherichia coli 
                 
                 2.0 
               
               
                   
                 base# 
                   
                   
                 SGD strain 
               
               
                 5 
                 — 
                 — 
                 10 
                 
                   escherichia coli 
                 
                 2.0 
               
               
                   
                   
                   
                   
                 SGD strain 
               
               
                 6 
                 — 
                 — 
                 10 
                 — 
                 — 
               
               
                   
               
               
                 Note 
               
               
                 *All the test doses were calculated by the enrofloxacin; 
               
               
                 #the administration way of the test group of the enrofloxacin base was that feeding was performed twice through a gastric tube in the morning and the evening daily according to the feed intake of each test pig. 
               
             
          
         
       
     
         [0033]    After artificial infection with an  escherichia coli  SGD strain, all the test pigs in the control group without administration but with challenge (the fifth group) showed diarrhea, wherein 6 pigs died in the test period, while all the test pigs in the control group without the administration and without challenge (the sixth group) grew normally without diarrhea and death. All the medication administration groups showed complete protection effects against  escherichia coli  artificial infection (See table 16 for the results). 
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                 TABLE 16 
               
             
             
               
                   
               
               
                 Protection effects of enrofloxacin in different 
               
               
                 forms against  escherichia coli  challenge infection 
               
             
          
           
               
                   
                   
                 Number 
                   
                 Incidence rate 
                   
               
               
                   
                   
                 of test 
                 Challenge 
                 (%, calculated 
                 Death rate 
               
               
                 Group 
                 Test sample 
                 pigs 
                 (Yes/No) 
                 by diarrhea) 
                 (%) 
               
               
                   
               
             
          
           
               
                 1 
                 enrofloxacin 
                 10 
                 Yes 
                 0 
                 0 
               
               
                   
                 zinc 
               
               
                 2 
                 enrofloxacin 
                 10 
                 Yes 
                 0 
                 0 
               
               
                   
                 copper 
               
               
                 3 
                 enrofloxacin 
                 10 
                 Yes 
                 0 
                 0 
               
               
                   
                 silver 
               
               
                 4 
                 enrofloxacin 
                 10 
                 Yes 
                 0 
                 0 
               
               
                   
                 base #   
               
               
                 5 
                 — 
                 10 
                 Yes 
                 100 
                 60 
               
               
                 6 
                 — 
                 10 
                 No 
                 0 
                 0