Abstract:
The present invention pertains to the prevention or lessening of disease in cats caused by Feline Immunodeficiency Virus (FIV). Prevention or lessening of disease is understood to mean the amelioration of any symptoms, including immune system disruptions, that result from FIV infection. The invention provides recombinant proteins produced in a baculovirus expression system having at least one internal gene comprising a DNA sequence that encodes either the entire FIV env region (ENV-ABC), or a DNA sequence consisting of viral nucleotides 6252-8469 of FIV env (ENV-AB), a DNA sequence consisting of viral nucleotides 6252-6722 and 8264-9140 of FIV env (ENV-AC), or immunogenic fragments of any of the foregoing either singly or in combination. By immunogenic fragment is meant any portion of the coding sequence of FIV env polypeptides that induces a beneficial immune response in cats. In another embodiment, the invention encompasses vaccines that comprise one or more of the baculovirus-expressed recombinant FIV proteins described above, with a pharmaceutically acceptable carrier or diluent and a pharmaceutically acceptable adjuvant. In yet another aspect, the invention provides methods for preventing or lessening disease caused by FIV, which is carried out by administering to a feline in need of such treatment the vaccines described above.

Description:
FIELD OF THE INVENTION 
     The present invention pertains to the treatment and prophylaxis of disease caused by feline immunodeficiency virus (FIV), using baculovirus expressed envelope protein of FIV (env), or immunogenic fragments thereof, as vaccines. 
     BACKGROUND OF THE INVENTION 
     Feline immunodeficiency virus (FIV) infection is a significant health problem for domestic cats around the world. As in its human counterpart, infection with FIV causes a progressive disruption in immune function. In the acute phase of infection, the virus causes transient illness associated with symptoms such as lymphadenopathy, pyrexia, and neutropenia. Subsequently, an infected animal enters an asymptomatic phase of 1-2 years before clinical manifestations of immune deficiency become apparent, after which the mean survival time is usually less than one year. 
     FIV is a typical retrovirus that contains a single-stranded polyadenylated RNA genome, internal structural proteins derived from the gag gene product, and a lipid envelope containing membrane proteins derived from the env gene product (Bendinelli et al., Clin.Microbiol.Rev. 8:87, 1995). The env gene yields a primary translation product of 75-80 kDa (unglycosylated molecular weight); in infected cells, the precursor has an apparent molecular weight of 145-150 kDa due to N-linked glycosylation. The env precursor is cleaved in the Golgi apparatus into the SU and TM proteins (also designated gp95 and gp40, respectively). 
     Most vaccines against FIV have failed to induce protective immunity. Ineffective vaccines have involved inactivated whole virus, fixed infected cells, recombinant CA and SU proteins, and a synthetic peptide corresponding to the V3 region of SU. In some cases, the vaccine actually enhanced infection after challenge. In one system, vaccination with paraformaldehyde-fixed virus or infected cells resulted in protective immunity (Yamamoto et al., J. Virol. 67:601, 1993), but application of this approach by others was unsuccessful (Hosie et al., in Abstracts of the International Symposium on Feline Retrovirus Research, 1993, page 50). 
     Thus, there is a need in the art for an effective vaccine against FIV that utilizes env proteins, or fragments thereof, as immunogens. 
     SUMMARY OF THE INVENTION 
     The present invention pertains to the prevention or lessening of disease in cats caused by Feline Immunodeficiency Virus (FIV). Prevention or lessening of disease is understood to mean the amelioration of any symptoms, including immune system disruptions, that result from FIV infection. 
     The invention provides recombinant proteins produced in a baculovirus expression system having at least one internal gene comprising a DNA sequence that encodes either the entire FIV env region (ENV-ABC), or a DNA sequence consisting of viral nucleotides 6252-8469 of FIV env (ENV-AB), a DNA sequence consisting of viral nucleotides 6252-6722 and 8264-9140 of FIV env (ENV-AC), or immunogenic fragments of any of the foregoing either singly or in combination. By immunogenic fragment is meant any portion of the coding sequence of FIV env polypeptides that induces a beneficial immune response in cats. 
     In another embodiment, the invention encompasses vaccines that comprise one or more of the baculovirus-expressed recombinant FIV proteins described above, with a pharmaceutically acceptable carrier or diluent and a pharmaceutically acceptable adjuvant. 
     In yet another aspect, the invention provides methods for preventing or lessening disease caused by FIV, which is carried out by administering to a feline in need of such treatment the vaccines described above. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 is a graphic illustration of the cloning strategy for the env genes of FIV which involved individually cloning DNA sequences encoding aminoterminal, internal, and carboxyterminal segments of the FIV env gene, followed by stepwise ligation of all three segments to produce an intact env-encoding DNA sequence. 
     FIG. 2 is a diagrammatic representation of construction comprising the FIV envelope gene detailing the start and endpoints of the ENV-A, ENV-B, and ENV-C fragments in relation to the pre-envelope sequence, the envelope leader signal, the surface (SU) protein, and the transmembrane (TM) protein. 
     FIG. 3 shows the DNA sequence of the env genes cloned from FIV (NCSU -1 strain)  SEQ. I.D. NO. 7!. 
     FIG. 4 shows the amino acid sequence of the env proteins of FIV as predicted from the DNA sequence of FIG. 3  SEQ. I.D. NO. 8!. 
     FIG. 5 is a graphic illustration of the subcloning strategy for the FIV env genes into the baculovirus transfer plasmid, pVL-WENV. 
     FIG. 6 is a table illustrating the detection of viremia and CD4:CD8 ratios in vaccinated and unvaccinated cats of Experiment 1 after FIV challenge. 
     FIG. 7 is a table illustrating the preventable fraction for viremia and CD4:CD8 ratio changes in vaccinated and unvaccinated cats following FIV challenge. 
     FIG. 8 is a table illustrating the detection of viremia and CD4:CD8 ratios in vaccinated and unvaccinated cats of Experiment 2 after FIV challenge. 
     FIG. 9 is a table illustrating the preventable fraction for viremia and CD4:CD8 changes in vaccinated and unvaccinated cats of Experiment 2 after FIV challenge. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     All patents, patent applications, and references cited herein are hereby incorporated by reference in their entirety. In the case of inconsistencies, the present disclosure, including definitions, will control. 
     The vaccine of the present invention may be prepared by creating recombinant baculovirus containing a gene encoding the env proteins of Feline Immunodeficiency Virus (FIV) or immunogenic fragments thereof. Env genes useful in practicing the present invention may be obtained by methods well-known in the art. In one embodiment, viral RNA is reverse-transcribed using endogenous or exogenous reverse transcriptase and the DNA is rendered double-stranded using DNA polymerase. The env-encoding DNA segments are then recovered by restriction enzyme digestion and are amplified by cloning in E. coli. In another embodiment, FIV-infected cat cells serve as a source of FIV proviral DNA. In this embodiment, chromosomal DNA is isolated from the cells, and oligonucleotide primers are used to specifically amplify the env genes or fragments therefrom using polymerase chain reaction. This approach is broadly applicable to purifying env genes from different FIV strains or isolates, since primers can be designed from non-polymorphic regions of the FIV genome. The only requirement for FIV env genes for use in the present invention is that the DNA contain sequences that encode env polypeptides, or immunogenic fragments thereof. 
     FIV env genes isolated by the above methods are first inserted into a transfer plasmid, and the recombinant plasmid is co-transfected with a nuclear polyhedrosis virus linear DNA into appropriate host cells. The DNA from the transfer plasmid is incorporated into the polyhedrosis virus DNA by homologous recombination, and the recombinant viruses are screened for lack of polyhedra and the presence of the desired FIV coding sequence and immunological detection of the desired protein. 
     DNA encoding the FIV env proteins or fragments therefrom are inserted into a transfer plasmid downstream of a baculovirus promoter. In a preferred embodiment, the polyhedrin protein promoter of baculovirus is used. However, alternate promoter elements which function in insect cells could be used. In the preferred transfer vector, the inserted FIV gene is flanked by DNA sequences which facilitate integration of the plasmid DNA sequences into the polyhedrosis virus DNA by homologous recombination. 
     In a further embodiment of the present invention, baculovirus can be produced that express less-than-full-length segments of the FIV env proteins. The techniques used to engineer transfer plasmids encoding partial sequences of env are well-known and widely used in the art, as are the methods for production and screening of recombinant baculovirus as detailed in this specification. For example, convenient restriction enzyme recognition sites can be used to obtain fragments of the gene, as described, e.g., Example 1 below. Alternatively, introduction of oligonucleotides containing a stop codon at various points along env DNA will produce a nested set of carboxyterminal-truncated versions of that gene, which can then be incorporated into the transfer vector. Furthermore, sequences that encode different domains on each protein may be recombined, using domains derived from different FIV strains or isolates. It will be apparent to one of ordinary skill in the art that systematic screening of such recombinant baculovirus can establish whether the intact protein, subfragments thereof or multi-strain recombinants thereof, are most preferred in practicing the present invention. Furthermore, as stated above, DNA encoding different fragments of env can be used in a combination vaccine after incorporation into the same, or different, recombinant baculoviruses. 
     For vaccine preparation, the selected recombinant virus is grown on susceptible cells. Cells are infected with recombinant baculovirus at a multiplicity of infection of 0.05 to 20, preferably 1.0 in 50 ml of media. Cells are separated from supernatant, and the supernatant is then inactivated, and concentrated. Cell material is extracted and cell debris is pelleted. The vaccine is preferably made by combining equal aliquots of both supernatant and cell material. Supernatant and/or cell material or a mixture thereof can be frozen or lyophilized until use. 
     The amount of supernatant and/or cell material to be used in the vaccine of the present invention is a immunogenically effective amount, generally the amount derived from a culture having a titer of at least 10 4  TCID 50  /ml and generally no greater than 10 7  TCID 50  /ml. However, determination of the immunogenically effective amount is within the purview of one of ordinary skill, and is determined, for example by performing a matrix experiment varying the amount of supernatant/cell material and assaying for immunological reaction, as described below. 
     Supernatant and cell material from insect cell infected with multiple viruses encoding multiple env genes or fragments thereof may be mixed together for vaccination. Furthermore, the virus may be mixed with additional inactivated or attenuated viruses, bacteria, or fungi such as feline leukemia virus, feline panleukopenia virus, feline rhinotracheitis virus, feline calicivirus, feline infectious peritonitis virus, feline Chlamydia psittaci, Microsporum canis, or others. In addition, antigens from the above-cited organisms may be incorporated into combination vaccines. These antigens may be purified from natural sources or from recombinant expression systems, or may comprise individual subunits of the antigen or synthetic peptides derived therefrom. 
     An additional optional component of the present vaccine is a pharmaceutically acceptable adjuvant. Non-limiting examples of suitable adjuvants include squalane and squalene (or other oils of animal origin); block copolymers such as Pluronic® (L121) Saponin; detergents such as Tween®-80; Quil® A, mineral oils such as Drakeol® or Marcol®, vegetable oils such as peanut oil; Corynebacterium-derived adjuvants such as corynebacterium parvum; Propionibacterium-derived adjuvants such as Propionibacterium acne; Mycobacterium bovis (Bacillus Calmette and Guerinn, or BCG); interleukins such as interleukin 2 and interleukin-12; monokines such as interleukin 1; tumor necrosis factor; interferons such as gamma interferon; combinations such as saponin-aluminum hydroxide or Quil®-A aluminum hydroxide; liposomes; iscom adjuvant; mycobacterial cell wall extract; synthetic glycopeptides such as muramyl dipeptides or other derivatives; Avridine; Lipid A; dextran sulfate; DEAE-Dextran or DEAE-Dextran with aluminum phosphate; carboxypolymethylene, such as Carbopol®; EMA; acrylic copolymer emulsions such as Neocryle® A640 (e.g. U.S. Pat. No. 5,047,238); vaccinia or animal poxvirus proteins; subviral particle adjuvants such as orbivirus; cholera toxin; dimethyldiocledecylammonium bromide; or mixtures thereof. 
     In a further embodiment of the present invention, baculovirus cell lysates can be incorporated into liposomes, or encapsulated in peptide-, protein-, or polysaccharide-based microcapsules prior to administration, using means that are known in the art. 
     At the time of vaccination, the supernatant, cell material or a mixture thereof is thawed (if frozen) or reconstituted (if lyophilized) with a physiologically-acceptable carrier or diluent such as deionized water, saline, phosphate buffered saline, or the like. 
     The vaccine of the present invention is administered to cats in volumes ranging from 0.5 to 5 milliliters. The vaccine can be administered to cats by subcutaneous, intramuscular, oral, intradermal, or intranasal routes. The number of injections and their temporal spacing may be varied. One to three vaccinations administered at intervals of one to four weeks are usually effective. 
     The efficacy of the vaccines of the present invention is assessed by the following methods. At about two weeks after the final vaccination, vaccinates and controls are each challenged, preferably through subcutaneous inoculation with 3-20 cat ID 50  units, preferably 5 cat ID 50  units of FIV, and preferably the NCSU-1 isolate. Whole blood is obtained from the animals immediately before challenge, and at intervals after challenge, for measurement of a) viremia and b) relative amounts of CD4 and CD8 lymphocytes. 
     Viremia is measured by isolating mononuclear cells from the blood, and co-culturing the cells with mononuclear cells from uninfected animals. After 7 days of culture, the culture supernatant are tested for FIV by enzyme-linked immunoassay (see Example 4 below). 
     The ratio of CD4 to CD8 lymphocytes in the circulation of vaccinates and controls is taken as a measure of immune function. Typically, FIV infection causes an inversion of the normal CD4:CD8 ratio of about 1.5-4.0 to a pathological ratio of about 0.5-1.0. The titers of CD4 and CD8 lymphocytes are measured by flow cytometry using specific antibodies (see Example 4 below). 
     Another measure of immune function is to challenge vaccinates and controls with Toxoplasma gondii at 6 months-12 months after the final vaccination. Normally, the severity of T. gondii- induced disease symptoms is considerably exacerbated in FIV-infected cats relative to uninfected cats. The severity of the T. gondii effect is determined by scoring ocular discharge, nasal discharge, dyspnea, and fever. 
     It will be understood that amelioration of any of the symptoms of FIV infection is a desirable clinical goal. This includes a lessening of the dosage of medication used to treat FIV-induced symptoms. 
     The following examples are intended to illustrate the present invention without limitation thereof. 
     EXAMPLE 1 
     Generation of Recombinant Baculovirus Expressing FIV ENV Gene 
     A. Preparation of FIV DNA 
     FIV-NCSU-1 was isolated from a naturally infected, feline leukemia virus-negative cat and has been described previously (M. B. Tompkins et al. J. Am. Vet. Med. Assoc. 199: 1311-1315, 1991). The virus was passed in a normal specific pathogen-free (SPF) cat (obtained from Liberty Laboratories, Waverly, N.Y.) and FIV-infected peripheral blood mononuclear cells (PBMC) were obtained from whole blood by separation on discontinuous percoll gradients. Briefly, anti-coagulated whole blood was layered over a two step gradient containing 43% Percoll™ (Pharmacia, Piscataway, N.J.) over 62.5% Percoll® diluted in 0.15M NaCl. Gradients were centrifuged at 400×g for 5 minutes followed by 800×g for 20 minutes at 22° C. PBMC were harvested from the gradient interface and washed in phosphate buffered saline containing 5% fetal bovine serum. 
     FIV was propagated by culture of PBMC&#39;s from the FIV-infected cat with PBMC&#39;s from normal cats in RPMI 1640 media containing 10% fetal bovine serum, 2.5×10-5 beta-mercaptoethanol, 2 mM L-glutamine, 5 μg/mL concanavalin A, and 20% conditioned media from MLA cells (ATCC TIB 201) as a source of interleukin-2 (IL-2). Cat genomic DNA containing FIV-NCSU-1 proviral sequences was isolated from these cultured cells by lysis of the cells with 0.6% sodium dodecyl sulfate (SDS) in 10 mM Tris-HCl, pH 7.5,. 10 mM EDTA buffer. Chromosomal DNA was precipitated by incubation overnight with 1 mM NaCl followed by centrifugation at 10,000 r.p.m. (Beckman J2, JA-20 rotor) for 40 minutes. The pellet was resuspended in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.1% SDS buffer and digested with ribonuclease A (20 μg/ml) and proteinase K (0.2 mg/ml) at 50° C. for 4 hours. DNA was then purified by sequential extraction with phenol, phenol:chloroform (1:1) and chloroform followed by ethanol precipitation. 
     B. Cloning of FIV Envelope Sequences 
     FIV-NCSU-1 envelope DNA sequences were cloned using Polymerase Chain Reaction methods as follows. 
     1. Envelope Fragment A (ENV-A) Coding strand (6252-V): 5&#39; TCGGATCCAACAATAATTATGGCAGAAGG 3&#39; SEQ. I.D. NO. 1! Complementary strand (6745-C) 5&#39; AATCAGGTACAAAGTCACCGTTC 3&#39; SEQ. I.D. NO. 2! 
     Primer 6252-V corresponds to nucleotides 6252-6273 of FIV strain PPR (GenBank No. M36968) and primer 6745-C (underlined region) corresponds to nucleotides 6723-6745 of FIV strain 14 (GenBank No. 25381). The start codon for envelope protein translation is included in primer 6252-V. Primer 6252-V also has a BamHI restriction enzyme site near the 5&#39; end to facilitate cloning. An AvrII site located at position 6719 also facilitates cloning. Envelope fragment A is 494 bp. 
     2. Envelope Fragment B (ENV-B) Coding strand (6637-V) 5&#39; TATAGAAGCACCCCAAGAAGAG 3&#39; SEQ, I.D. NO. 3! Complementary strand (8469-C) 5&#39; CATTCCCCCAAAGTTATATTTC 3&#39; SEQ. I.D. NO. 4! 
     Primers 6637-V and 8469-C correspond to nucleotides 6637-6659 and 8448-8469 of FIV 14 strain, respectively. An AvrII site at position 6719 and a SpeI site at position 8288 facilitated cloning. Envelope fragment B is 1833 bp. 
     3. Envelope Fragment C (ENV-C) Coding strand (8264-V) 5&#39; TTAGTTACATTAGAGCATCAAG 3&#39; SEQ. I.D. NO. 5! Complementary strand (9145-C) 5&#39; TTCTAGATCTTCAGGGTCCCAATACTC 3&#39; SEQ. I.D. NO. 6! 
     Primer 8264-V corresponds to nucleotides 8264-8285 of FIV strain 14 and primer 9145-C (underlined region) corresponds to nucleotides 9126-9145 of FIV strain PPR. Primer 9145-C has a BglII site near the 5&#39; end to facilitate cloning. A Spel site located at position 8288 also facilitated cloning. Envelope fragment C is 880 bp. 
     PCR was performed for 35 cycles of 1 min 30 sec at 94° C., 2 min at 56° C., and 2 min at 72° C., followed by one cycle of 8 min at 72° C. Each envelope fragment was isolated by gel electrophoresis and cloned into plasmid pSL1190 using standard methods (Maniatis et al., Molecular Cloning: A Laboratory Manual, 1982, Cold Spring Harbor Press). Envelope A fragment was cut with BamHI and AvrII, envelope B with AvrII and SpeI, and envelope C with SpeI and BglII. Each fragment was then inserted into pSL1190 (FIG. 1). Splicing of the three envelope fragments was done to generate a full length envelope gene. The 1.5 kbp AvrII/SpeI envelope B fragment was-ligated into AvrII/SpeI cut pSL-EnvA to create pSL-EnvAB (FIG. 1). The envAB fragment codes for the entire surface membrane protein (SU) and the first 63 amino acids from the amino-terminus of the transmembrane protein (TM) of FIV-NCSU-1. However, envAB does not contain the membrane spanning domain of TM. 
     Next, the 0.9 kbp SpeI/SmaI envelope C fragment from pSL-EnvC was ligated into SpeI/BbrPI cut pSL-EnvAB to make pSL-EnvABC or pSL-WEnv (FIG. 1). The WEnv fragment codes for the entire env open reading frame (SU and TM proteins) of FIV NCSU-1 (FIG. 2). 
     A plasmid containing the &#34;A&#34; region plus the &#34;C&#34; region of the FIV envelope was also created. pSL-ENV-AC was made by ligating the 0.8 kb SPEI/NdeI Env C fragment of pSLENVC into AVRII/NdeI cut pSL-ENV-A (FIG. 1). 
     The subcloned genetic elements of FIV-NCSU-1 were sequenced using Sequenase Version 2.0 (United States Biochemical, Cleveland, Ohio)) as described for double-stranded DNA, and the reactions were analyzed using the ABI automated sequencer (Applied Biosystems, Foster City, Calif.). Both DNA strands were sequenced to confirm the results. The DNA sequences were analyzed using the MacVector DNA Analysis software (International Biotechnologies, Inc., New Haven, Conn.). The env DNA sequences were analyzed for open reading frames and compared to the previously published DNA sequences of other FIV isolates. The DNA and predicted amino acid sequences of env and envAB open reading frames of FIV-NCSU-1 are shown in FIGS. 3 and 4. 
     EXAMPLE 2 
     Preparation of Baculovirus Expressing FIV-Envelope 
     The full-length FIV-NCSU-1 gene from the pSL-WENV plasmid was cleaved using BamH1 and BglII and subcloned into the BamH1/BglII cleaved baculovirus transfer plasmid pVL1393 (InVitrogen, San Diego, Calif.) downstream of the polyhedrin promoter (plasmid pVL3-WENV, see FIG. 4). Spodoptera frugiperda 9 (Sf9) insect cells were co-transfected with pVL3-WENV plasmid and Autographa californica nuclear polyhedrosis virus (AcMNPV) linear DNA according to manufacturer&#39;s instructions (InVitrogen, San Diego, Calif.). Recombinant AcMNPV viruses were identified by lack of polyhedra and plaque-purified on Sf9 cells. Virus clone 7.1.4a was selected for preparation of a master seed (BAC-ENV) based on the presence of FIV envelope gene sequences as detected by polymerase chain reaction (using baculovirus primers  forward -44 and reverse +778! and procedure from InVitrogen, San Diego, Calif.) and expression of envelope protein by immunoblot using hyperimmune goat antisera raised against FIV. 
     A. Preparation of Baculovirus Expressing the AB portion of FIV-Envelope 
     The AB fragment of the FIV-NCSU-1 envelope gene from the pSL-EnvAB plasmid was cleaved using BamH1 and SpeI and subcloned into the SmaI/BamHI cleaved baculovirus transfer plasmid pVL1393 (InVitrogen, San Diego, Calif.) downstream of the polyhedrin promotor. Transfection and selection of recombinant virus was performed as for BAC-ENV. Virus clone 1.1a was selected for preparation of a master seed (BAC-AB) based on the presence of FIV envelope gene sequences as detected by polymerase chain reaction (using baculovirus primers and procedure from InVitrogen, San Diego, Calif.) and expression of envelope protein by immunoblot using hyperimmune goat antisera raised against FIV. 
     B. Preparation of Baculovirus Expressing the AC portion of FIV-Envelope 
     The AC fragment of the FIV-NCSU-1 envelope gene from the pSL-ENV-AC plasmid was cleaved using BamHI and PstI and subcloned into the BamHI/PstI cut baculovirus transfer plasmid pVL1393. Transfection and selection for recombinant virus was performed as for BAC-ENV. 
     EXAMPLE 3 
     Production of Vaccines 
     BAC-ENV virus was grown on Spodoptera frugiperda 21 (SF21) cells (InVitrogen, San Diego, Calif.) as follows: Ten 850 cm 2  roller bottles were seeded with approximately 2×10 7  SF21 cells in 100 mL of ExCell 401 media (JRH Biosciences, Lenexa, Kans.) and incubated for 18 hours at 27° C. BAC-ENV seed (at 2 passages beyond the master seed virus) was added to the cells at a multiplicity of infection (MOI) of approximately 1.0 in 50 mL of ExCell 401 media and incubated for 1 hour. After this absorption, each roller bottle was adjusted to 200 mL with ExCell 401. After seven days incubation at 27° C., cells and supernatant were harvested and inactivated with 2 mM binary ethylenimine for 3 days at 37° C. Harvested material was centrifuged at 5000×g for 30 minutes in order to remove cell material. The supernatant was concentrated 20-fold through a 10,000 NMW hollow fiber concentrator (Amicon, Beverly, Mass.). The cell fraction was extracted with 100 mL of 0.5% (V/V) Tween-20 (polyoxyethenesorbitan monolaurate), 0.5% (V/V) Triton X100 (polyoxyethylene ether) in phosphate buffered saline for one hour at 22° C. using gentle tumbling, followed by centrifugation at 5000×g for 30 minutes to pellet cell debris. Equal volumes of cell extract and concentrated supernatant were used to blend vaccine. 
     BAC-AB virus fluids were prepared as described for BAC-ENV. 
     BAC-AC virus fluids were prepared as described for BAC-ENV. 
     
         ______________________________________   BAC-ENV   Vaccine BAC-AB Vaccine                        BAC-AC Vaccine______________________________________20X Super 10.5 mL   9.5 mL       10.5 mL20X Cell Ex.     10.5 mL   9.5 mL       10.5 mLAdjuvant A      1.5 mL   1.0 mL        1.5 mLDEAE Dextran      7.5 mL   --            7.5 mLTotal Volume       30 mL    20 mL         30 mL______________________________________ 
    
     Adjuvant A is an adjuvant comprising a block copolymer, such as a polyoxypropylene-polyoxyethylene (POP-POE) block copolymer, preferably Pluronic® L121 (e.g. U.S. Pat. No. 4,772,466), and an organic component, such as a metabolizable oil, e.g. an unsaturated turpin hydrocarbon, preferably squalane (2,6,10,15,19,23-hexamethyltetracosane) or squalene. The composition may also include a non-ionic detergent or surfactant, preferably a polyoxyethylene sorbitan monooleate such as a Tween® detergent, most preferably Tween®-80, i.e. polyoxyethylene (20) sorbitan monooleate. 
     In this adjuvant mixture, the block copolymer, organic oil, and surfactant may be present in amounts ranging from about 10 to about 40 ml/L, about 20 to about 80 ml/L, and about 1.5 to about 6.5 ml/L, respectively. In a preferred embodiment of the stock adjuvant, the organic component is squalane present in an amount of about 40 mL/L, the surfactant is polyoxyethylenesorbitan monooleate (Tween®-80) present in an amount of about 3.2 ml/L, and the POP-POE block copolymer is Pluronic® L121 present in an amount of about 20 ml/L. Pluronic® L121 is a liquid copolymer at 15-40° C., where the polyoxypropylene (POP) component has a molecular weight of 3250 to 4000 and the polyoxyethylene (POE) component comprises about 10-20%, preferably 10%, of the total molecule. 
     EXAMPLE 4 
     Vaccine Testing 
     A. Animals. 
     Specific-pathogen free (SPF) domestic shorthair cats (Liberty Laboratories, Waverly, N.Y.), both male and female that were greater than 6 months of age were used. 
     B. Vaccination. 
     Cats were vaccinated with a 1 mL dose of vaccine administered subcutaneously in the nape of the neck. A second vaccination was administered 21 days later using the same route and dosage. Five cats were vaccinated with each vaccine formulation. Ten cats remained as unvaccinated controls. 
     C. FIV Challenge. 
     Fourteen days after the second vaccination cats were challenged with 5 cat ID 50  of NCSU-1 virus by subcutaneous inoculation. Whole blood was obtained from the cats prior to challenge and periodically after challenge in order to assess virus infection parameters as follows: 
     1. Detection of Viremia 
     a. PCR Detection of FIV proviral DNA 
     Mononuclear cells were isolated from whole blood using Percoll™ (Pharmacia Biotech, Piscataway N.J.) gradients. 5×10 5  cells were lysed and 1/10th of the lysate used in a polymerase chain reaction assay with oligonucleotide primers specific to the gag gene of FIV (T L Wasmoen et al. Vet. Immun. Immunopath. 35: 83-93, 1992). FIV amplified DNA was detected by agarose gel electrophoresis and ethidium bromide staining. 
     b. Tissue Culture Isolation of FIV 
     Culture isolate of FIV was performed as described previously (Wasmoen et al., Vet. Immuno. Immunopath. 35:83-93, 1992). Mononuclear cells were isolated from whole blood using Percoll™ (Pharmacia Biotech, Piscataway N.J.) gradients. 5×10 5  cells from FIV-challenged cats were cultured with 1×10 6  mononuclear cells isolated from uninfected cats. Cultures were fed with RPMI media every 7 days and supernatant tested for the presence of FIV by an enzyme-linked immunosorbent assay (ELISA) that detects FIV p25 antigen (Petcheck ELISA, IDEXX, Portland ME). 
     2. Lymphocyte Subsets 
     Leukocytes were isolated from whole blood using Histopaque™ (Sigma Chemical Company, St. Louis Mo.) and lymphocyte subsets quantitated by staining the cells with antibodies specific to CD4 (monoclonal antibody CAT30A), CD8 (monoclonal antibody FLSM 3.357), pan T lymphocytes (monoclonal antibody FLSM 1.572) or B lymphocytes (anti-cat IgG) followed by FACS analysis. These monoclonal antibodies are described elsewhere (M. B. Tompkins et al. Vet. Immunol. Immunopathol. 26:305-317, 1990) and the flow cytometry procedure is the same as previously described (R. V. English et al. J. Infect. Dis. 170:543-552, 1994). CD4:CD8 ratios were calculated. 
     D. Results. 
     1. Experiment 1 
     Two vaccine formulations were tested in this experiment. Vaccine BAC-AC contains baculovirus-expressed envelope sequences from a portion of the N-terminus (outer envelope) and the C-terminus (transmembrane) of the NCSU-1 envelope protein, but lacks a major part of the outer envelope (SU) protein. Vaccine BAC-ENV contains the entire FIV envelope protein encoding sequence as a subunit expressed in baculovirus. Both vaccines were formulated using the same adjuvant. Cats were given two doses of vaccine and challenged with the NCSU-1 strain of FIV. Prior to challenge, all cats had normal CD4:CD8 lymphocyte ratios (i.e., values greater than 1.0) and where negative for FIV virus as detected by tissue culture isolations of virus or PCR detection of proviral DNA (Table 2, FIG. 6). Three months after FIV inoculation, 80% of the control cats were found to be infected with virus and the same cats had CD4:CD8 ratios that were lower than 1.0 (i.e., inverted). Cats vaccinated with BAC-AC showed a similar pattern with 80% infected with FIV virus and 60% showing CD4:CD8 inversions. By comparison, cats vaccinated with BAC-ENV were negative for viremia, however, one cat showed an abnormal CD4:CD8 lymphocyte ratio. Therefore, vaccine ENV-AC showed no prevention of viremia and only 25% prevention of CD4:CD8 inversions. On the other hands, BAC-ENV induced 100% prevention of viremia and 75% prevention of CD4:CD8 inversion (Table 3, FIG. 7). Because of the apparent lack of efficacy for the BAC-AC formulation, the cats in this group were euthanized and not evaluated at later time points in the study. 
     At eight months after FIV challenge, 80% of the controls, remained viremic and showed CD4:CD8 inversions (Table 2, FIG. 6). By comparison, only two of the BAC-ENV cats were viremic and only one showed a CD4:CD8 inversion (Table 2, FIG. 6). Therefore, the BAC-ENV vaccine showed 50% prevention of viremia, but still showed 70% prevention of CD4:CD8 inversions (Table 3, FIG. 7). At 18 months after FIV challenge, 80% of the controls remained FIV viremic and three of the animals with valid test data still showed CD4:CD8 inversions (Table 2, FIG. 6). By contrast, FIV viremia was detected in three of the BAC-ENV vaccinated cats and four of these cats still had CD4:CD8 ratios in the normal range (Table 2, FIG. 6). Therefore, the vaccine was less effective in preventing viremia at this later time point, however, it was at least 75% effective in preventing the CD4:CD8 ratio inversion associated with FIV infection. 
     2. Experiment 2 
     Five cats were vaccinated with a subunit vaccine containing all the outer envelope protein of FIV and a portion of the transmembrane region that was expressed in baculovirus. The response to FIV infection in these vaccinated cats were compared to the response in unvaccinated control cats. Prior to challenge, all cats were aviremic and possessed normal CD4:CD8 lymphocyte ratios (i.e., values greater than 1.0). By 1.5 months after challenge, 40% of control cats were viremic and showed CD4:CD8 lymphocyte ratio inversions (Table 4, FIG. 8). By contrast only one vaccinate was found to be viremic and none showed CD4:CD8 inversions (Table 4, FIG. 8). Therefore, at this early time point, the vaccine was 50% effective in preventing viremia and 100% effective in preventing lymphocyte subclass alterations (Table 5, FIG. 9). 
     At 2.5 to 3.5 months after FIV challenge, 60% of controls were infected with FIV and 80% had inverted CD4:CD8 ratios (Table 4, FIG. 8). Vaccinates, on the other hand, showed a 20% infection rate and a 40% lymphocyte inversion rate (Table 4, FIG. 8). Therefore, the vaccine was 67% effective in preventing viremia and 50% effective in preventing lymphocyte subset inversions (Table 5, FIG. 9). Cat EY1 in the vaccinated group died at 10 months after challenge of causes apparently unrelated to infection with FIV, because the cat had never shown signs of infection. However, this could not be confirmed by necropsy because of the poor state of the carcass when discovered the day after death. At 22 months after FIV inoculation, 80% of the controls were viremic and 60% had inverted CD4:CD8 lymphocyte ratios (Table 4, FIG. 8). Protection against infection had decreased in the vaccinates as 75% were now viremic and 50% showed 4:CD8 lymphocyte inversions. 
     In both experiments, the NCSU-1 strain of FIV was used to evaluate vaccine efficacy because this virus has been shown to be virulent in that it causes a persistent infection that leads to an immune deficiency disease in cats (English et al. J. Inf. Dis. 170:543-52, 1994). In the studies presented, persistent viremia and evidence of adverse changes to the immune system (based on inversions of CD4:CD8 lymphocyte ratios below 1.0) were evident in a consistent percentage of control cats. Vaccination with a baculovirus subunit vaccine containing the entire FIV envelope (BAC-ENV) was effective in preventing viremia and CD4:CD8 inversions in a significant portion of cats. Protection against infection was most evident within eight months of FIV challenge and appeared to deteriorate by 19 months. This may indicate that yearly booster vaccination will be necessary to maintain a high level of protective immunity. However, strong protection against CD4:CD8 lymphocyte inversions below 1.0 was evident even at the later time point. Therefore, even in vaccinated cats that were infected with virus, the alterations to the immune system were not as great as found in control cats. The minimum effective dose of antigen was 7 mL of baculovirus-infected whole culture (supernatant and extracted cells). 
     The vaccine containing the outer envelope protein but lacking the entire transmembrane coding region (BAC-AB) also showed improvement over controls for prevention of viremia and CD4:CD8 lymphocyte inversion at early time points after vaccination. The minimum effective dose of antigen was 9.5 mL of baculovirus-infected whole culture (supernatant and extracted cells) where the antigen was derived from a culture having a titer of at least 10 4  TCID 50  /ml. On the other hand, the BAC-AC vaccine which lacks the majority of the outer envelope coding region, showed essentially no efficacy in this model. 
     
         __________________________________________________________________________SEQUENCE LISTING(1) GENERAL INFORMATION:(iii) NUMBER OF SEQUENCES: 8(2) INFORMATION FOR SEQ ID NO:1:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 29 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(vi) ORIGINAL SOURCE:(A) ORGANISM: Feline immunodeficiency virus(B) STRAIN: PPR(viii) POSITION IN GENOME:(B) MAP POSITION: 6252-6273(C) UNITS: bp(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:TCGGATCCAACAATAATTATGGCAGAAGG29(2) INFORMATION FOR SEQ ID NO:2:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 23 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(iv) ANTI-SENSE: YES(vi) ORIGINAL SOURCE:(A) ORGANISM: feline immunodeficiency virus(B) STRAIN: 14(viii) POSITION IN GENOME:(B) MAP POSITION: 6723-6745(C) UNITS: bp(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:AATCAGGTACAAAGTCACCGTTC23(2) INFORMATION FOR SEQ ID NO:3:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 22 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(vi) ORIGINAL SOURCE:(A) ORGANISM: feline immunodeficiency virus(B) STRAIN: 14(viii) POSITION IN GENOME:(B) MAP POSITION: 6637-6659(C) UNITS: bp(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:TATAGAAGCACCCCAAGAAGAG22(2) INFORMATION FOR SEQ ID NO:4:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 22 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(iv) ANTI-SENSE: YES(vi) ORIGINAL SOURCE:(A) ORGANISM: feline immunodeficiency virus(B) STRAIN: 14(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:CATTCCCCCAAAGTTATATTTC22(2) INFORMATION FOR SEQ ID NO:5:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 22 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(vi) ORIGINAL SOURCE:(A) ORGANISM: feline immunodeficiency virus(B) STRAIN: 14(viii) POSITION IN GENOME:(B) MAP POSITION: 8264-8285(C) UNITS: bp(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:TTAGTTACATTAGAGCATCAAG22(2) INFORMATION FOR SEQ ID NO:6:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 27 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(iv) ANTI-SENSE: YES(vi) ORIGINAL SOURCE:(A) ORGANISM: feline immunodeficiency virus(B) STRAIN: PPR(viii) POSITION IN GENOME:(B) MAP POSITION: 9126-9145(C) UNITS: bp(xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:TTCTAGATCTTCAGGGTCCCAATACTC27(2) INFORMATION FOR SEQ ID NO:7:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 3225 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(vi) ORIGINAL SOURCE:(A) ORGANISM: feline immunodeficiency virus(C) INDIVIDUAL ISOLATE: NCSU-1(viii) POSITION IN GENOME:(B) MAP POSITION: 1-3225(C) UNITS: bp(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:GGATCCAACAATAATTATGGCAGAAGGATTTGCAGCCAATAGACAATGGATAGGACCAGA60AGAAGCTGAAGAGTTATTAGATTTTGATATAGCAACACAAATGAATGAAGAAGGGCCACT120AAATCCAGGGATGAACCCATTTAGGGTACCTGGAATAACAGATAAAGAAAAGCAAGACTA180TTGTAACATATTACAACCTAAGTTACAAGATTTACGGAATGAACTTCAAGAGGTAAAACT240AGAAGAAGGAAATGCAGGTAAGTTTAGAAGAACAAGATTTTTAAGGTATTCTGATGAACA300AGTATTGTCCCCGGTTCATGCGTTCATAGGATATTGTATTTATTTAGGTAATCGAAATAA360GTTAGGATCTTTAAGACATGACATTGATATTGAAGCACCCCCCGAAGAGTGTTATGATAA420TAGAGAGAAGGGTACAACTGACAATATAAAATATGGTAGACGATGTTGCCTAGGAACGGT480GACTTTGTACCTGATTTTATTTATAGGATTAATAATATATTCACAGACAGCCGACGCTCA540GGTAGTATGGAGACTTCCACCATTAGTAGTCCCAGTAGAAGAATCAGAAATAATTTTTTG600GGATTGTTGGGCACCAGAAGAACCCGCCTGTCAGGACTTTCTTGGGGCAATGATACATCT660AAAAGCTAAGACAAATATAAGTATACGAGAGGGACCTACCTTGGGGAATTGGGCTAGAGA720AATATGGGCAACATTATTCAAAAAGGCTACTAGACAATGTAGAAGAGGCAGAATATGGAA780AAGATGGGATGAGACTATAACAGGACCATCAGGATGTGCTAATAACACATGTTATAATGT840TTCAGCAATAGTACCTGATTATCAGCGTTATTTAGATAGAGTAGATACTTGGTTACAAGG900GAAAATAAATATATCATTATGTCTAACAGGAGGAAAAATGTTGTACAATAAAGTTACAAA960ACAATTAAGCTATTGTACAGACCCATTACAAATCCCACTGATCAATTATACATTTGGACC1020TAATCAAACATGTATGTGGAATACTTCACAAATTCAGGACCCTGAAATACCACAATGTGG1080ATGGTGGAATCACATGGCCTATTATAACAGTTGTAAATGGGAAGAGGCAAAGGTAAAGTT1140TCATTGTCAAAGAACACAGAGTCAGCCTGGGTCATGGCGTAGAGCAATCTCGTCATGGAA1200ACAAAGAAATAGATGGGAGTGGAGACCAGATTTTGAGAGTGAAAAGGTGAAAATATCTCT1260ACAGTGCAATAGCACGAAAAACCTAACCTTTGCAATGAGAAGTTCAGGAGATTATGGAGA1320AGTAACGGGAGCTTGGATAGAGTTTGGATGTCATAGAAATAAATCAAACCTTCATACTGA1380AGCAAGGTTTAGAATTAGATGTAGATGGAATGTAGGGAGTGATACCTCGCTCATTGATAC1440ATGTGGAAACACTCCAAATGTTTCAGGTGCGAATCCTGTAGATTGTACCATGTATTCAAA1500TAAAATGTACAAGTTTTCTTTACCAAACGGGTTTACAATGAAGGTAGATGACCTTATTAT1560GCATTTCAATATGCCAAAAGCTGTAGAAATGAATAATATTGCTGGAAATTGGTCTTGTAC1620ATCTGACTTGCCATCGTCATGGGGGTATATGAATTGTAATTGCCCAAATAGTAGTAGTAG1680TTATAGTGGTACTAAAATGGCATGTCCTAGCAATCGAGGCATCTTAAGGAATTGGTATAA1740CCCAGTAGCAGGATTACGACAATCCTTAGAACAGTATCAAGTTGTAAAACAACCAGATTA1800CTTACTGGTCCCAGAGGAAGTCATGGAATATAAACCTAGAAGGAAAAGGGCAGCTATTCA1860TGTTATGTTGGCTCTTGCAACAGTATTATCTATTGCCGGTGCAGGGACGGGGGCTACTGC1920TATAGGGATGGTAACACAATACCACCAAGTTCTGGCAACCCATCAAGAATCTATGGAAAA1980GGTGACTGAAGCCTTAGAGATAAACAACTTAAGGTTAGTTACATTAGAGCATCAAGTACT2040AGTAATAGGATTAAAAGTAGAAGCTATGGAAAAATTTTTATATACAGCTTTCGCTATGCA2100AGAATTAGGATGTAATCCAAATCAATTTTTCTCCAAAATCCCTCTTGAGTTGTGGACAAG2160GTATAATATGACTATAAATCAAACAATATGGAATCATGGAAATATAACTTTGGGGGAATG2220GTATAACCACACCAAAGATTTACAACCAAAGTTTTATGAAATAATAATGGACATAGAACC2280AAATAATGTACAAGGGAAAACAGGGATACAACAATTACCCAAGTGGGAAGATTGGGTAAG2340ATGGATAGGAAATATTCCACAATATTTAAAGGGACTATTGGGAGGTATCTTGGGAATAGG2400ATTAGGAGTGTTATTATTGATTTTATGTTTACCTACATTGGTTGATTGTATAAGAAATTG2460TATCCACAAGATACTAGGATACACAGTAATTGCAATGCCTGAAGTAGAAGGAGAAGAAAT2520ACAACCACAAATGGAATTGAGGAGAAATGGTAGCCAATTTGGCATGTCTGAAAAAGAGGA2580GGAATGATGAAGTATCTCAGACTTATTTTATAAGGGAGATACTGTGCTAAGTTCTTCCCT2640TTGAGGAAGGTATGTCATATGAATCCATTTCGAACCAAATCAAACTAATAAAGTATGTAT2700TGTAAGGTAAAAGGAAAAGACAAAGAAGAAGAAGAAAGAAGAAAGCTTTCAAGAGGATGA2760TGACAGAGTTAGAAGATCGCTTCAGGAAGCTATTTGGCACGACTTCTACAACGGGAGACA2820GCACAGTAGATTCTGAAGATGAACCTCCTAAAAAAGAAAAAAGGGTGGACTGGGATGAGT2880ATTGGAACCCTGAAGAAATAGAAAGAATGCTTATGGACTAGGGACTGTTTACGAACAAAT2940GATAAAAGGAAATAGCTAAGCATGACTCATAGTTAAAGCGCTAGCAGCTGCTTAACCGCA3000AAACCACATCCTATGTAAAGCTTGCTAATGACGTATAAGTTGTTCCATTGTAAGAGTATA3060TAACCAGTGCTTTGTGAAACTTCGAGGAGTCTCTCCGTTGAGGACTTTCGAGTTCTCCCT3120TGAGGCTCCCACAGATACAATAAATATTTGAGATTGAACCCTGTCAAGTATCTGTGTAAT3180CTTTTTTACCTGTGAGGTCTCGGAATCCGGGCCGAGAACTTCGCA3225(2) INFORMATION FOR SEQ ID NO:8:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 856 amino acids(B) TYPE: amino acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(vi) ORIGINAL SOURCE:(A) ORGANISM: feline immunodeficiency virus(C) INDIVIDUAL ISOLATE: NCSU-1(xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:MetAlaGluGlyPheAlaAlaAsnArgGlnTrpIleGlyProGluGlu151015AlaGluGluLeuLeuAspPheAspIleAlaThrGlnMetAsnGluGlu202530GlyProLeuAsnProGlyMetAsnProPheArgValProGlyIleThr354045AspLysGluLysGlnAspTyrCysAsnIleLeuGlnProLysLeuGln505560AspLeuArgAsnGluLeuGlnGluValLysLeuGluGluGlyAsnAla65707580GlyLysPheArgArgThrArgPheLeuArgTyrSerAspGluGlnVal859095LeuSerProValHisAlaPheIleGlyTyrCysIleTyrLeuGlyAsn100105110ArgAsnLysLeuGlySerLeuArgHisAspIleAspIleGluAlaPro115120125ProGluGluCysTyrAspAsnArgGluLysGlyThrThrAspAsnIle130135140LysTyrGlyArgArgCysCysLeuGlyThrValThrLeuTyrLeuIle145150155160LeuPheIleGlyLeuIleIleTyrSerGlnThrAlaAspAlaGlnVal165170175ValTrpArgLeuProProLeuValValProValGluGluSerGluIle180185190IlePheTrpAspCysTrpAlaProGluGluProAlaCysGlnAspPhe195200205LeuGlyAlaMetIleHisLeuLysAlaLysThrAsnIleSerIleArg210215220GluGlyProThrLeuGlyAsnTrpAlaArgGluIleTrpAlaThrLeu225230235240PheLysLysAlaThrArgGlnCysArgArgGlyArgIleTrpLysArg245250255TrpAspGluThrIleThrGlyProSerGlyCysAlaAsnAsnThrCys260265270TyrAsnValSerAlaIleValProAspTyrGlnArgTyrLeuAspArg275280285ValAspThrTrpLeuGlnGlyLysIleAsnIleSerLeuCysLeuThr290295300GlyGlyLysMetLeuTyrAsnLysValThrLysGlnLeuSerTyrCys305310315320ThrAspProLeuGlnIleProLeuIleAsnTyrThrPheGlyProAsn325330335GlnThrCysMetTrpAsnThrSerGlnIleGlnAspProGluIlePro340345350GlnCysGlyTrpTrpAsnHisMetAlaTyrTyrAsnSerCysLysTrp355360365GluGluAlaLysValLysPheHisCysGlnArgThrGlnSerGlnPro370375380GlySerTrpArgArgAlaIleSerSerTrpLysGlnArgAsnArgTrp385390395400GluTrpArgProAspPheGluSerGluLysValLysIleSerLeuGln405410415CysAsnSerThrLysAsnLeuThrPheAlaMetArgSerSerGlyAsp420425430TyrGlyGluValThrGlyAlaTrpIleGluPheGlyCysHisArgAsn435440445LysSerAsnLeuHisThrGluAlaArgPheArgIleArgCysArgTrp450455460AsnValGlySerAspThrSerLeuIleAspThrCysGlyAsnThrPro465470475480AsnValSerGlyAlaAsnProValAspCysThrMetTyrSerAsnLys485490495MetTyrLysPheSerLeuProAsnGlyPheThrMetLysValAspAsp500505510LeuIleMetHisPheAsnMetProLysAlaValGluMetAsnAsnIle515520525AlaGlyAsnTrpSerCysThrSerAspLeuProSerSerTrpGlyTyr530535540MetAsnCysAsnCysProAsnSerSerSerSerTyrSerGlyThrLys545550555560MetAlaCysProSerAsnArgGlyIleLeuArgAsnTrpTyrAsnPro565570575ValAlaGlyLeuArgGlnSerLeuGluGlnTyrGlnValValLysGln580585590ProAspTyrLeuLeuValProGluGluValMetGluTyrLysProArg595600605ArgLysArgAlaAlaIleHisValMetLeuAlaLeuAlaThrValLeu610615620SerIleAlaGlyAlaGlyThrGlyAlaThrAlaIleGlyMetValThr625630635640GlnTyrHisGlnValLeuAlaThrHisGlnGluSerMetGluLysVal645650655ThrGluAlaLeuGluIleAsnAsnLeuArgLeuValThrLeuGluHis660665670GlnValLeuValIleGlyLeuLysValGluAlaMetGluLysPheLeu675680685TyrThrAlaPheAlaMetGlnGluLeuGlyCysAsnProAsnGlnPhe690695700PheSerLysIleProLeuGluLeuTrpThrArgTyrAsnMetThrIle705710715720AsnGlnThrIleTrpAsnHisGlyAsnIleThrLeuGlyGluTrpTyr725730735AsnHisThrLysAspLeuGlnProLysPheTyrGluIleIleMetAsp740745750IleGluProAsnAsnValGlnGlyLysThrGlyIleGlnGlnLeuPro755760765LysTrpGluAspTrpValArgTrpIleGlyAsnIleProGlnTyrLeu770775780LysGlyLeuLeuGlyGlyIleLeuGlyIleGlyLeuGlyValLeuLeu785790795800LeuIleLeuCysLeuProThrLeuValAspCysIleArgAsnCysIle805810815HisLysIleLeuGlyTyrThrValIleAlaMetProGluValGluGly820825830GluGluIleGlnProGlnMetGluLeuArgArgAsnGlySerGlnPhe835840845GlyMetSerGluLysGluGluGlu850855__________________________________________________________________________