Abstract:
The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. In particular, the disclosure relates to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and a glutamine synthetase

Description:
CROSS REFERENCE TO RELATED APPLICATION 
       [0001]    This application claims the benefit of U.S. Provisional Application No. 61/308,974, filed Feb. 28, 2010, which is hereby incorporated by reference in its entirety. 
     
    
     STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT 
       [0002]    This invention was made with government support under Contract No. DE-AC52-06NA25396, awarded by the United States Department of Energy to Los Alamos National Security, LLC. The government has certain rights in this invention. 
     
    
     SUBMISSION OF SEQUENCE LISTING ON ASCII TEXT FILE 
       [0003]    The content of the following submission on ASCII text file is incorporated herein by reference in its entirety: a computer readable form (CRF) of the Sequence Listing (file name: 686522000500SEQLIST.txt, date recorded: Feb. 25, 2011, size: 132 KB). 
       FIELD 
       [0004]    The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. The disclosure also relates to recombinant polynucleotides for the generation of transgenic algae having increased growth characteristics. 
       BACKGROUND OF THE INVENTION 
       [0005]    The global biodiesel market demand is estimated to reach 37 billion gallons by 2016, growing at an average annual growth rate of 42%. Europe will be the major market for the next decade or so, closely followed by the US market. To meet this increased market demand, additional oil sources, especially non-edible oils, need to be explored (Li et al., 2008 Appl. Microbiol. Biotechnol. 80:749-756). Microalgae seems to be the only source of renewable biodiesel that has the potential to displace petroleum-derived transportation fuels without the controversial argument “Food or Fuel” and to help the nation reach the 2003 Biofuels Directive target of achieving greenhouse gas savings (Christi, 2007, Biotechnol. Adv. 25:294-306; Christi, 2008, Trends Biotechnol. 26:126-131; Cockerill and Martin, 2008, Biotechnol. Biofuels 1:9). 
         [0006]    The most advanced biotechnology being applied to algal growth has been the creation of the antennae mutants that have less light harvesting machinery in the cell, which allows a greater fraction of the light to pass through an individual cell. This light then strikes other cells deeper in the culture. This is viewed as advantageous because some of the light energy striking a normal cell is in excess and is lost as fluorescence. These mutants do not suffer this loss of excess energy; it is available to other deeper cells in the culture. Thus the overall culture accumulates biomass faster. These mutants then grow using their normal rates of metabolism. In addition, some are attempting to engineer herbicide resistance genes into the production strains to allow competing algae in a production bioreactor to be controlled with the herbicide. 
         [0007]    Numerous algal biofuels companies populate the landscape; it is reasonable to expect at least 20 of them will be producing algal oil at large scale within a year. Microalgal biodiesel is technically feasible (Gouveia et al. 2009 J. Ind. Microbiol. Biotechnol 36:269-274). However technoeconomic analyses show that for microalgal biofuels to be economically competitive with petrodiesel, the production, harvesting and extraction steps must be optimized and costs reduced. The production step must be increased substantially to increase the overall total biomass production. The degree to which the production rate can be improved within the constraints of the fixed costs of the production reactor, will dictate how much other costs must be reduced to achieve profitability or even the bottom line. The technology described herein can be expected to address that need. 
         [0008]    In plants, the organic compound 2-oxoglutaramate is a powerful signal metabolite which regulates the function of a large number of genes involved in the photosynthesis apparatus, carbon fixation and nitrogen metabolism. A number of transaminase and hydrolyase enzymes known to be involved in the synthesis of 2-hydroxy-5-oxoproline in animals have been identified in animal liver and kidney tissues (Cooper and Meister, 1977, CRC Critical Reviews in Biochemistry, pages 281-303; Meister, 1952, J. Biochem. 197:304). In algae, the biochemical synthesis of 2-hydroxy-5-oxoproline has not been established. Moreover, the function of 2-hydroxy-5-oxoproline in algae is unknown. 
         [0009]    Unkefer et al., U.S. Pat. No. 6,593,275, disclose a dramatic increase in the growth rate of algae when treated with 2-hydroxy-5-oxoproline. Continuously culturing the algae in the presence of this compound or mixtures of this compound with other prolines will enrich sub-strains of the algae that respond well to the prolines. 
       SUMMARY OF THE INVENTION 
       [0010]    The present disclosure relates to transgenic algae having increased growth characteristics. In one embodiment, the invention relates to transgenic algae having enhanced (faster) growth rates. Applicants have recently identified the enzyme glutamine phenylpyruvate transaminase (GPT) as a catalyst of 2-hydroxy-5-oxoproline (2-oxoglutaramate) synthesis in plants, and here disclose that transgenic algae engineered to over-express plant-derived GPT and glutamine synthetase (GS1) genes grow faster and produce higher amounts of chlorophyll compared to wild type algae. 
         [0011]    In one embodiment, the disclosure provides the generation of GPT+GS1  Chlorella . The double-transgenic  Chlorella  demonstrated substantially faster growth rates that the untransformed  Chlorella  grown under identical conditions for the same amount of time. Methods for the generation of the transgenic algae of the invention are provided. 
         [0012]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter. 
         [0013]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, and wherein the algae is a green algae. 
         [0014]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, and wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species. 
         [0015]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, and wherein the GPT transgene is a plant-derived GPT. 
         [0016]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, and wherein the GPT is an algal-derived GPT. 
         [0017]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, and wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47. 
         [0018]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, and wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48. 
         [0019]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, and wherein the GS transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 39, an amino acid sequence that is at least 75% identical to SEQ ID NO: 4, an amino acid sequence that is at least 75% identical to SEQ ID NO: 7, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 39. 
         [0020]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0021]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the algae is a green algae, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0022]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0023]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0024]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0025]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0026]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0027]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GS transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 39, an amino acid sequence that is at least 75% identical to SEQ ID NO: 4, an amino acid sequence that is at least 75% identical to SEQ ID NO: 7, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 39, and wherein the GPT and GS transgenes are incorporated into the genome of the algae. 
         [0028]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0029]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the algae is a green algae, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0030]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0031]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0032]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0033]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0034]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0035]    In one embodiment, the disclosure provides the progeny of any generation of a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GS transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 39, an amino acid sequence that is at least 75% identical to SEQ ID NO: 4, an amino acid sequence that is at least 75% identical to SEQ ID NO: 7, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 39, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene and said GS transgene. 
         [0036]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0037]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the algae is a green algae, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0038]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0039]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0040]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0041]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0042]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0043]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene and a GS transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GS transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 39, an amino acid sequence that is at least 75% identical to SEQ ID NO: 4, an amino acid sequence that is at least 75% identical to SEQ ID NO: 7, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 39, wherein the GPT and GS transgenes are incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0044]    In one embodiment, the disclosure provides a method for increasing growth characteristics of an algae relative to a wild type or progenitor algae of the same species, the method including: (a) introducing a GPT transgene into the algae; (b) introducing a GS transgene into the algae or a progeny of the algae; (c) expressing the GPT transgene and the GS transgene in the algae or the progeny of the algae; and, (d) selecting an algae having an increased growth characteristic relative to an algae of the same species that does not contain a GPT transgene or a GS transgene. 
         [0045]    In one embodiment, the disclosure provides a method for increasing growth characteristics of an algae relative to a wild type or progenitor algae of the same species, the method including: (a) introducing a GPT transgene into the algae; (b) introducing a GS transgene into the algae or a progeny of the algae; (c) expressing the GPT transgene and the GS transgene in the algae or the progeny of the algae; and, (d) selecting an algae having an increased growth characteristic relative to an algae of the same species that does not contain a GPT transgene or a GS transgene, and wherein the increased growth characteristic is selected from the group consisting of: faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions. 
         [0046]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter. 
         [0047]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the algae is a green algae. 
         [0048]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species. 
         [0049]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the GPT transgene is a plant-derived GPT. 
         [0050]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the GPT is an algal-derived GPT. 
         [0051]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, and wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47. 
         [0052]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the GPT is an algal-derived GPT, and wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48. 
         [0053]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the GPT transgene is incorporated into the genome of the algae. 
         [0054]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the algae is a green algae, and wherein the GPT transgene is incorporated into the genome of the algae. 
         [0055]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species, and wherein the GPT transgene is incorporated into the genome of the algae. 
         [0056]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, and wherein the GPT transgene is incorporated into the genome of the algae. 
         [0057]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, and wherein the GPT transgene is incorporated into the genome of the algae. 
         [0058]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47, and wherein the GPT transgene is incorporated into the genome of the algae. 
         [0059]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the GPT is an algal-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48, and wherein the GPT transgene is incorporated into the genome of the algae. 
         [0060]    In one embodiment, the disclosure provides a progeny of any generation of a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene. 
         [0061]    In one embodiment, the disclosure provides a progeny of any generation of a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the algae is a green algae, wherein the GPT transgene is incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene. 
         [0062]    In one embodiment, the disclosure provides a progeny of any generation of a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species, wherein the GPT transgene is incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene. 
         [0063]    In one embodiment, the disclosure provides a progeny of any generation of a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene is incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene. 
         [0064]    In one embodiment, the disclosure provides a progeny of any generation of a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, wherein the GPT transgene is incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene. 
         [0065]    In one embodiment, the disclosure provides a progeny of any generation of a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47, wherein the GPT transgene is incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene. 
         [0066]    In one embodiment, the disclosure provides a progeny of any generation of a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the GPT is an algal-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48, wherein the GPT transgene is incorporated into the genome of the algae, and wherein said progeny comprises said GPT transgene. 
         [0067]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0068]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the algae is a green algae, wherein the GPT transgene is incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0069]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the algae is selected from a  Chlorella  or  Chlamydomonas  species, wherein the GPT transgene is incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0070]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene is incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0071]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT is an algal-derived GPT, wherein the GPT transgene is incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0072]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, wherein the GPT transgene is a plant-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 9, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO 24, SEQ ID NO: 30, SEQ ID NO:31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 44, SEQ ID NO: 47, an amino acid sequence that is at least 75% identical to SEQ ID NO: 2, an amino acid sequence that is at least 75% identical to SEQ ID NO: 9, an amino acid sequence that is at least 75% identical to SEQ ID NO: 15, an amino acid sequence that is at least 75% identical to SEQ ID NO: 19, an amino acid sequence that is at least 75% identical to SEQ ID NO: 21, an amino acid sequence that is at least 75% identical to SEQ ID NO 24, an amino acid sequence that is at least 75% identical to SEQ ID NO: 30, an amino acid sequence that is at least 75% identical to SEQ ID NO:31, an amino acid sequence that is at least 75% identical to SEQ ID NO: 32, an amino acid sequence that is at least 75% identical to SEQ ID NO: 33, an amino acid sequence that is at least 75% identical to SEQ ID NO: 34, an amino acid sequence that is at least 75% identical to SEQ ID NO: 35, an amino acid sequence that is at least 75% identical to SEQ ID NO: 36, an amino acid sequence that is at least 75% identical to SEQ ID NO: 44, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 47, wherein the GPT transgene is incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0073]    In one embodiment, the disclosure provides a transgenic algae including a GPT transgene, wherein said GPT transgene is operably linked to a promoter, and wherein the GPT is an algal-derived GPT, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NO: 48, and an amino acid sequence that is at least 75% identical to SEQ ID NO: 48, wherein the GPT transgene is incorporated into the genome of the algae, and wherein the transgenic algae displays a faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions when compared to an analogous wild-type or untransformed algae. 
         [0074]    In one embodiment, the disclosure provides a method for increasing growth characteristics of an algae relative to an wild type or progenitor algae of the same species, the method including: (a) introducing a GPT transgene into the algae; (b) expressing the GPT transgene in the algae or the progeny of the algae; and, (c) selecting an algae having an increased growth characteristic relative to an algae of the same species that does not comprise a GPT transgene. 
         [0075]    In one embodiment, the disclosure provides a method for increasing growth characteristics of an algae relative to an wild type or progenitor algae of the same species, the method including: (a) introducing a GPT transgene into the algae; (b) expressing the GPT transgene in the algae or the progeny of the algae; and, (c) selecting an algae having an increased growth characteristic relative to an algae of the same species that does not comprise a GPT transgene, wherein the increased growth characteristic is selected from the group consisting of: faster growth rate, increased chlorophyll production, increased ribulose bisphosphate carboxylase (RUBISCO) level, increased nitrogen use efficiency, increased biomass yield, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels, and/or increased tolerance to salt or saline conditions. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0076]      FIG. 1 . Photograph of tissue culture plates, showing GPT+GS1 transformed  Chlorella vulgaris  on the right, and untransformed  Chlorella vulgaris  (control) on the left. See, Example 1, infra. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Definitions 
       [0077]    Unless otherwise defined, all terms of art, notations and other scientific terminology used herein are intended to have the meanings commonly understood by those of skill in the art to which this invention pertains. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such definitions herein should not necessarily be construed to represent a substantial difference over what is generally understood in the art. The techniques and procedures described or referenced herein are generally well understood and commonly employed using conventional methodology by those skilled in the art, such as, for example, the widely utilized molecular cloning methodologies described in Sambrook et al., Molecular Cloning: A Laboratory Manual 3rd. edition (2001) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; Current Protocols in Molecular Biology (Ausbel et al., eds., John Wiley &amp; Sons, Inc. 2001). As appropriate, procedures involving the use of commercially available kits and reagents are generally carried out in accordance with manufacturer defined protocols and/or parameters unless otherwise noted. 
         [0078]    The term “nucleic acid” refers to deoxyribonucleotides or ribonucleotides and polymers thereof (“polynucleotides”) in either single- or double-stranded form. Unless specifically limited, the term “polynucleotide” encompasses nucleic acids containing known analogues of natural nucleotides which have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g. degenerate codon substitutions) and complementary sequences and as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., 1991, Nucleic Acid Res. 19:5081; Ohtsuka et al., 1985 J. Biol. Chem. 260:2605-2608; and Cassol et al., 1992; Rossolini et al., 1994, Mol. Cell. Probes 8:91-98). The term nucleic acid is used interchangeably with gene, cDNA, and mRNA encoded by a gene. 
         [0079]    The term “promoter” refers to a nucleic acid control sequence or sequences that direct transcription of an operably linked nucleic acid. Promoters include necessary nucleic acid sequences near the start site of transcription, such as, in the case of a polymerase II type promoter, a TATA element. A promoter also optionally includes distal enhancer or repressor elements, which can be located as much as several thousand base pairs from the start site of transcription. A “constitutive” promoter is a promoter that is active under most environmental and developmental conditions. An “inducible” promoter is a promoter that is active under environmental or developmental regulation. The term “operably linked” refers to a functional linkage between a nucleic acid expression control sequence (such as a promoter, or array of transcription factor binding sites) and a second nucleic acid sequence, wherein the expression control sequence directs transcription of the nucleic acid corresponding to the second sequence. 
         [0080]    The terms “polypeptide,” “peptide” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers and non-naturally occurring amino acid polymers. 
         [0081]    The term “amino acid” refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids. Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, γ-carboxyglutamate, and O-phosphoserine. Amino acid analogs refers to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an a carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, e.g., homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid. Amino acid mimetics refers to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions in a manner similar to a naturally occurring amino acid. 
         [0082]    Amino acids may be referred to herein by either their commonly known three letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Nucleotides, likewise, may be referred to by their commonly accepted single-letter codes. 
         [0083]    The term “algae” refers to photosynthetic organisms of multiple phylogenetic groups, and includes numerous unicellular and multicellular species. The term “algae” as used herein includes organisms of the following phylogenetic groups:  Chlorophyta  (green algae; includes mostly fresh water species);  Phaeophyta  (brown algae; includes mostly marine species);  Rhodophyta  (red algae; includes mostly marine species);  Chrysophyta; Xanthophyta; Bacillariophyta; Euglenophyta; Cryptophyta; Pyrrophyta; Raphidophyta; Haptophyta; Eustigmatophyta; Prasinophyta; Glaucophyta  and  Cyanobacteria  (prokaryotic, blue-green algae). The class of algae which can be used in the methods of the invention is generally as broad as the class of algae amenable to transformation techniques. 
         [0084]    Algae of the present disclosure include but are not limited to organisms of the following genera:  Acanthoceras, Acanthococcus, Acaryochloris, Achnanthes, Achnanthidium, Actinastrum, Actinochloris, Actinocyclus, Actinotaenium, Amphichrysis, Amphidinium, Amphikrikos, Amphipleura, Amphiprora, Amphithrix, Amphora, Anabaena, Anabaenopsis, Aneumastus, Ankistrodesmus, Ankyra, Anomoeoneis, Apatococcus, Aphanizomenon, Aphanocapsa, Aphanochaete, Aphanothece, Apiocystis, Apistonema, Arthrodesmus, Artherospira, Ascochloris, Asterionella, Asterococcus, Audouinella, Aulacoseira, Bacillaria, Balbiania, Bambusina, Bangia, Basichlamys, Batrachospermum, Binuclearia, Bitrichia, Blidingia, Botrdiopsis, Botrydium, Botryococcus, Botryosphaerella, Brachiomonas, Brachysira, Brachytrichia, Brebissonia, Bulbochaete, Bumilleria, Bumilleriopsis, Caloneis, Calothrix, Campylodiscus, Capsosiphon, Carteria, Catena, Cavinula, Centritractus, Centronella, Ceratium, Chaetoceros, Chaetochloris, Chaetomorpha, Chaetonella, Chaetonema, Chaetopeltis, Chaetophora, Chaetosphaeridium, Chamaesiphon, Chara, Characiochloris, Characiopsis, Characium, Charales, Chilomonas, Chlainomonas, Chlamydoblepharis, Chlamydocapsa, Chlamydomonas, Chlamydomonopsis, Chlamydomyxa, Chlamydonephris, Chlorangiella, Chlorangiopsis, Chlorella, Chlorobotrys, Chlorobrachis, Chlorochytrium, Chlorococcum, Chlorogloea, Chlorogloeopsis, Chlorogonium, Chlorolobion, Chloromonas, Chlorophysema, Chlorophyta, Chlorosaccus, Chlorosarcina, Choricystis, Chromophyton, Chromulina, Chroococcidiopsis, Chroococcus, Chroodactylon, Chroomonas, Chroothece, Chrysamoeba, Chrysapsis, Chrysidiastrum, Chrysocapsa, Chrysocapsella, Chrysochaete, Chrysochromulina, Chrysococcus, Chrysocrinus, Chrysolepidomonas, Chrysolykos, Chrysonebula, Chrysophyta, Chrysopyxis, Chrysosaccus, Chrysophaerella, Chrysostephanosphaera, Clodophora, Clastidium, Closteriopsis, Closterium, Coccomyxa, Cocconeis, Coelastrella, Coelastrum, Coelosphaerium, Coenochloris, Coenococcus, Coenocystis, Colacium, Coleochaete, Collodictyon, Compsogonopsis, Compsopogon, Conjugatophyta, Conochaete, Coronastrum, Cosmarium, Cosmioneis, Cosmocladium, Crateriportula, Craticula, Crinalium, Crucigenia, Crucigeniella, Cryptoaulax, Cryptomonas, Cryptophyta, Ctenophora, Cyanidioschyzon, Cyanodictyon, Cyanonephron, Cyanophora, Cyanophyta, Cyanothece, Cyanothomonas, Cyclonexis, Cyclostephanos, Cyclotella, Cylindrocapsa, Cylindrocystis, Cylindrospermum, Cylindrotheca, Cymatopleura, Cymbella, Cymbellonitzschia, Cystodinium Dactylococcopsis, Debarya, Denticula, Dermatochrysis, Dermocarpa, Dermocarpella, Desmatractum, Desmidium, Desmococcus, Desmonema, Desmosiphon, Diacanthos, Diacronema, Diadesmis, Diatoma, Diatomella, Dicellula, Dichothrix, Dichotomococcus, Dicranochaete, Dictyochloris, Dictyococcus, Dictyosphaerium, Didymocystis, Didymogenes, Didymosphenia, Dilabifilum, Dimorphococcus, Dinobryon, Dinococcus, Diplochloris, Diploneis, Diplostauron, Distrionella, Docidium, Draparnaldia, Dunaliella, Dysmorphococcus, Ecballocystis, Elakatothrix, Ellerbeckia, Encyonema, Enteromorpha, Entocladia, Entomoneis, Entophysalis, Epichrysis, Epipyxis, Epithemia, Eremosphaera, Euastropsis, Euastrum, Eucapsis, Eucocconeis, Eudorina, Euglena, Euglenophyta, Eunotia, Eustigmatophyta, Eutreptia, Fallacia, Fischerella, Fragilaria, Fragilariforma, Franceia, Frustulia, Curcilla, Geminella, Genicularia, Glaucocystis, Glaucophyta, Glenodiniopsis, Glenodinium, Gloeocapsa, Gloeochaete, Gloeochrysis, Gloeococcus, Gloeocystis, Gloeodendron, Gloeomonas, Gloeoplax, Gloeothece, Gloeotila, Gloeotrichia, Gloiodictyon, Golenkinia, Golenkiniopsis, Gomontia, Gomphocymbella, Gomphonema, Gomphosphaeria, Gonatozygon, Gongrosia, Gongrosira, Goniochloris, Gonium, Gonyostomum, Granulochloris, Granulocystopsis, Groenbladia, Gymnodinium, Gymnozyga, Gyrosigma, Haematococcus, Hafniomonas, Hallassia, Hammatoidea, Hannaea, Hantzschia, Hapalosiphon, Haplotaenium, Haptophyta, Haslea, Hemidinium, Hemitoma, Heribaudiella, Heteromastix, Heterothrix, Hibberdia, Hildenbrandia, Hillea, Holopedium, Homoeothrix, Hormanthonema, Hormotila, Hyalobrachion, Hyalocardium, Hyalodiscus, Hyalogonium, Hyalotheca, Hydrianum, Hydrococcus, Hydrocoleum, Hydrocoryne, Hydrodictyon, Hydrosera, Hydrurus, Hyella, Hymenomonas, Isthmochloron, Johannesbaptistia, Juranyiella, Kappaphycus Karayevia, Kathablepharis, Katodinium, Kephyrion, Keratococcus, Kirchneriella, Klebsormidium, Kolbesia, Koliella, Komarekia, Korshikoviella, Kraskella, Lagerheimia, Lagynion, Laminaria, Lamprothamnium, Lemanea, Lepocinclis, Leptosira, Lobococcus, Lobocystis, Lobomonas, Luticola, Lyngbya, Malleochloris, Mallomonas, Mantoniella, Marssoniella, Martyana, Mastigocoleus, Gastogloia, Melosira, Merismopedia, Mesostigma, Mesotaenium, Micractinium, Micrasterias, Microchaete, Microcoleus, Microcystis, Microglena, Micromonas, Microspora, Microthamnion, Mischococcus, Monochrysis, Monodus, Monomastix, Monoraphidium, Monostroma, Mougeotia, Mougeotiopsis, Myochloris, Myromecia, Myxosarcina, Naegeliella, Nannochloris, Nautococcus, Navicula, Neglectella, Neidium, Nephroclamys, Nephrocytium, Nephrodiella, Nephroselmis, Netrium, Nitella, Nitellopsis, Nitzschia, Nodularia, Nostoc, Ochromonas, Oedogonium, Oligochaetophora, Onychonema, Oocardium, Oocystis, Opephora, Ophiocytium, Orthoseira, Oscillatoria, Ostreococcus, Oxyneis, Pachycladella, Palmella, Palmodictyon, Pnadorina, Pannus, Paralia, Pascherina, Paulschulzia, Pediastrum, Pedinella, Pedinomonas, Pedinopera, Pelagodictyon, Penium, Peranema, Peridiniopsis, Peridinium, Peronia, Petroneis, Phacotus, Phacus, Phaeaster, Phaeodactylum Phaeodermatium, Phaeophyta, Phaeosphaera, Phaeothamnion, Phormidium, Phycopeltis, Phyllariochloris, Phyllocardium, Phyllomitas, Pinnularia, Pitophora, Placoneis, Planctonema, Planktosphaeria, Planothidium, Plectonema, Pleodorina, Pleurastrum, Pleurocapsa, Pleurocladia, Pleurodiscus, Pleurosigma, Pleurosira, Pleurotaenium, Pocillomonas, Podohedra, Polyblepharides, Polychaetophora, Polyedriella, Polyedriopsis, Polygoniochloris, Polyepidomonas, Polytaenia, Polytoma, Polytomella, Porphyra, Porphyridium, Posteriochromonas, Prasinochloris, Prasinocladus, Prasinophyta, Prasiola, Prochlorphyta, Prochlorothrix, Protoderma, Protosiphon, Provasoliella, Prymnesium, Psammodictyon, Psammothidium, Pseudanabaena, Pseudenoclonium, Psuedocarteria, Pseudochate, Pseudocharacium, Pseudococcomyxa, Pseudodictyosphaerium, Pseudokephyrion, Pseudoncobyrsa, Pseudoquadrigula, Pseudosphaerocystis, Pseudostaurastrum, Pseudostaurosira, Pseudotetrastrum, Pteromonas, Punctastruata, Pyramichlamys, Pyramimonas, Pyrrophyta, Quadrichloris, Quadricoccus, Quadrigula, Radiococcus, Radiofilum, Raphidiopsis, Raphidocelis, Raphidonema, Raphidophyta, Peimeria, Rhabdoderma, Rhabdomonas, Rhizoclonium, Rhodomonas, Rhodophyta, Rhoicosphenia, Rhopalodia, Rivularia, Rosenvingiella, Rossithidium, Roya, Scenedesmus, Scherffelia, Schizochlamydella, Schizochlamys, Schizomeris, Schizothrix, Schroederia, Scolioneis, Scotiella, Scotiellopsis, Scourfieldia, Scytonema, Selenastrum, Selenochloris, Sellaphora, Semiorbis, Siderocelis, Diderocystopsis, Dimonsenia, Siphononema, Sirocladium, Sirogonium, Skeletonema, Sorastrum, Spermatozopsis, Sphaerellocystis, Sphaerellopsis, Sphaerodinium, Sphaeroplea, Sphaerozosma, Spiniferomonas, Spirogyra, Spirotaenia, Spirulina, Spondylomorum, Spondylosium, Sporotetras, Spumella, Staurastrum, Stauerodesmus, Stauroneis, Staurosira, Staurosirella, Stenopterobia, Stephanocostis, Stephanodiscus, Stephanoporos, Stephanosphaera, Stichococcus, Stichogloea, Stigeoclonium, Stigonema, Stipitococcus, Stokesiella, Strombomonas, Stylochrysalis, Stylodinium, Styloyxis, Stylosphaeridium, Surirella, Sykidion, Symbiodinium, Symploca, Synechococcus, Synechocystis, Synedra, Synochromonas, Synura, Tabellaria, Tabularia, Teilingia, Temnogametum, Tetmemorus, Tetrachlorella, Tetracyclus, Tetradesmus, Tetraedriella, Tetraedron, Tetraselmis, Tetraspora, Tetrastrum, Thalassiosira, Thamniochaete, Thorakochloris, Thorea, Tolypella, Tolypothrix, Trachelomonas, Trachydiscus, Trebouxia, Trentepholia, Treubaria, Tribonema, Trichodesmium, Trichodiscus, Trochiscia, Tryblionella, Ulothrix, Uroglena, Uronema, Urosolenia, Urospora, Uva, Vacuolaria, Vaucheria, Volvox, Volvulina, Westella, Woloszynskia, Xanthidium, Xanthophyta, Xenococcus, Zygnema, Zygnemopsis , and  Zygonium.    
         [0085]    The terms “GPT polynucleotide” and “GPT nucleic acid” are used interchangeably herein, and refer to a full length or partial length polynucleotide sequence of a gene which encodes a polypeptide involved in catalyzing the synthesis of 2-oxoglutaramate, and includes polynucleotides containing both translated (coding) and un-translated sequences, as well as the complements thereof. The term “GPT coding sequence” refers to the part of the gene which is transcribed and encodes a GPT protein. The term “targeting sequence” refers to the amino terminal part of a protein which directs the protein into a subcellular compartment of a cell, such as a chloroplast. GPT polynucleotides are further defined by their ability to hybridize under defined conditions to the GPT polynucleotides specifically disclosed herein, or to PCR products derived therefrom. 
         [0086]    A “GPT transgene” is a nucleic acid molecule including a GPT polynucleotide which is exogenous to a transgenic algae harboring the nucleic acid molecule, or which is exogenous to an ancestor algae, of a transgenic algae harboring the GPT polynucleotide. More particularly, the exogenous GPT transgene will be heterogeneous with any GPT polynucleotide sequence present in wild-type algae into which the GPT transgene is inserted. To this extent, the scope of the heterogeneity required need only be a single nucleotide difference. However, preferably the heterogeneity will be in the order of an identity between sequences selected from the following identities: 0.01%, 0.05%, 0.1%, 0.5%, 1%, 5%, 10%, 15%, and 20%. 
         [0087]    The terms “GS polynucleotide” and “GS nucleic acid” are used interchangeably herein, and refer to a full length or partial length polynucleotide sequence of a gene which encodes a glutamine synthetase protein, and includes polynucleotides containing both translated (coding) and un-translated sequences, as well as the complements thereof. The term “GS coding sequence” refers to the part of the gene which is transcribed and encodes a GS protein. The terms “GS1 polynucleotide” and “GS1 nucleic acid” are used interchangeably herein, and refer to a full length or partial length polynucleotide sequence of a gene which encodes a glutamine synthetase isoform 1 protein, and includes polynucleotides containing both translated (coding) and un-translated sequences, as well as the complements thereof. The term “GS1 coding sequence” refers to the part of the gene which is transcribed and encodes a GS1 protein. 
         [0088]    A “GS transgene” is a nucleic acid molecule including a GS polynucleotide which is exogenous to a transgenic algae, or which is exogenous to an ancestor algae of a transgenic algae harboring the GS polynucleotide. A “GS1 transgene” is a nucleic acid molecule including a GS1 polynucleotide which is exogenous to a transgenic algae harboring the nucleic acid molecule, or which is exogenous to an ancestor algae of a transgenic algae harboring the GS1 polynucleotide. More particularly, the exogenous GS or GS1 transgene will be heterogeneous with any GS or GS1 polynucleotide sequence present in wild-type algae into which the GS or GS1 transgene is inserted. To this extent the scope of the heterogeneity required need only be a single nucleotide difference. However, preferably the heterogeneity will be in the order of an identity between sequences selected from the following identities: 0.01%, 0.05%, 0.1%, 0.5%, 1%, 5%, 10%, 15%, and 20%. 
         [0089]    Exemplary GPT from algae, higher plants and a fish are presented herein, and include GPT sequences derived from  Chlorella, Arabidopsis , Rice, Barley, Bamboo o , Soybean, Grape, and Zebra Fish. GS and GS1 proteins are known; exemplary GS1 sequences provided herein include  Arabidopsis  and  Hordeum.    
         [0090]    Partial length GPT polynucleotides include polynucleotide sequences encoding N- or C-terminal truncations of GPT, mature GPT (without targeting sequence) as well as sequences encoding domains of GPT. Exemplary GPT polynucleotides encoding N-terminal truncations of GPT include  Arabidopsis − 30, −45 and −56 constructs, in which coding sequences for the first 30, 45, and 56, respectively, amino acids of the full length GPT structure of SEQ ID NO: 2 are eliminated. 
         [0091]    In employing the GPT polynucleotides of the invention in the generation of transformed algal cells and transgenic algae, one of skill will recognize that the inserted polynucleotide sequence need not be identical, but may be only “substantially identical” to a sequence of the gene from which it was derived, as further defined below. The term “GPT polynucleotide” specifically encompasses such substantially identical variants. Similarly, one of skill will recognize that because of codon degeneracy, a number of polynucleotide sequences will encode the same polypeptide, and all such polynucleotide sequences are meant to be included in the term GPT polynucleotide. In addition, the term specifically includes those sequences substantially identical (determined as described below) with a GPT polynucleotide sequence disclosed herein and that encode polypeptides that are either mutants of wild type GPT polypeptides or retain the function of the GPT polypeptide (e.g., resulting from conservative substitutions of amino acids in a GPT polypeptide). The term “GPT polynucleotide” therefore also includes such substantially identical variants. 
         [0092]    The term “conservatively modified variants” applies to both amino acid and nucleic acid sequences. With respect to particular nucleic acid sequences, conservatively modified variants refers to those nucleic acids which encode identical or essentially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. Because of the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For instance, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without altering the encoded polypeptide. Such nucleic acid variations are “silent variations,” which are one species of conservatively modified variations. Every nucleic acid sequence herein which encodes a polypeptide also describes every possible silent variation of the nucleic acid. One of skill will recognize that each codon in a nucleic acid (except AUG, which is ordinarily the only codon for methionine, and TGG, which is ordinarily the only codon for tryptophan) can be modified to yield a functionally identical molecule. Accordingly, each silent variation of a nucleic acid which encodes a polypeptide is implicit in each described sequence. 
         [0093]    As to amino acid sequences, one of skill will recognize that individual substitutions, deletions or additions to a nucleic acid, peptide, polypeptide, or protein sequence which alters, adds or deletes a single amino acid or a small percentage of amino acids in the encoded sequence is a “conservatively modified variant” where the alteration results in the substitution of an amino acid with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are well known in the art. Such conservatively modified variants are in addition to and do not exclude polymorphic variants, interspecies homologs, and alleles of the invention. 
         [0094]    The following eight groups each contain amino acids that are conservative substitutions for one another: 1) Alanine (A), Glycine (G); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (1), Leucine (L), Methionine (M), Valine (V); 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 7) Serine (S), Threonine (T); and 8) Cysteine (C), Methionine (M) (see, e.g., Creighton, Proteins (1984)). 
         [0095]    Macromolecular structures such as polypeptide structures can be described in terms of various levels of organization. For a general discussion of this organization, see, e.g., Alberts et al., Molecular Biology of the Cell (3 rd  ed., 1994) and Cantor and Schimmel,  Biophysical Chemistry Part  1:  The Conformation of Biological Macromolecules  (1980). “Primary structure” refers to the amino acid sequence of a particular peptide. “Secondary structure” refers to locally ordered, three dimensional structures within a polypeptide. These structures are commonly known as domains. Domains are portions of a polypeptide that form a compact unit of the polypeptide and are typically 25 to approximately 500 amino acids long. Typical domains are made up of sections of lesser organization such as stretches of β-sheet and α-helices. “Tertiary structure” refers to the complete three dimensional structure of a polypeptide monomer. “Quaternary structure” refers to the three dimensional structure formed by the noncovalent association of independent tertiary units. Anisotropic terms are also known as energy terms. 
         [0096]    The term “isolated” refers to material which is substantially or essentially free from components which normally accompany the material as it is found in its native or natural state. However, the term “isolated” is not intended refer to the components present in an electrophoretic gel or other separation medium. An isolated component is free from such separation media and in a form ready for use in another application or already in use in the new application/milieu. An “isolated” antibody is one that has been identified and separated and/or recovered from a component of its natural environment. Contaminant components of its natural environment are materials that would interfere with diagnostic or therapeutic uses for the antibody, and may include enzymes, hormones, and other proteinaceous or non-proteinaceous solutes. In preferred embodiments, the antibody will be purified (1) to greater than 95% by weight of antibody as determined by the Lowry method, and most preferably more than 99% by weight, (2) to a degree sufficient to obtain at least 15 residues of N-terminal or internal amino acid sequence by use of a spinning cup sequenator, or (3) to homogeneity by SDS-PAGE under reducing or nonreducing conditions using Coomassie blue or, preferably, silver stain. Isolated antibody includes the antibody in situ within recombinant cells since at least one component of the antibody&#39;s natural environment will not be present. Ordinarily, however, isolated antibody will be prepared by at least one purification step. 
         [0097]    The term “heterologous” when used with reference to portions of a nucleic acid indicates that the nucleic acid comprises two or more subsequences that are not found in the same relationship to each other in nature. For instance, a nucleic acid is typically recombinantly produced, having two or more sequences from unrelated genes arranged to make a new functional nucleic acid, e.g., a nucleic acid encoding a protein from one source and a nucleic acid encoding a peptide sequence from another source. Similarly, a heterologous protein indicates that the protein comprises two or more subsequences that are not found in the same relationship to each other in nature (e.g., a fusion protein). 
         [0098]    The terms “identical” or percent “identity,” in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same (i.e., about 70% identity, preferably 75%, 80%, 85%, 90%, or 95% identity over a specified region, when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using a sequence comparison algorithms, or by manual alignment and visual inspection. This definition also refers to the complement of a test sequence, which has substantial sequence or subsequence complementarity when the test sequence has substantial identity to a reference sequence. This definition also refers to the complement of a test sequence, which has substantial sequence or subsequence complementarity when the test sequence has substantial identity to a reference sequence. 
         [0099]    When percentage of sequence identity is used in reference to polypeptides, it is recognized that residue positions that are not identical often differ by conservative amino acid substitutions, where amino acids residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the polypeptide. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. 
         [0100]    For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters. 
         [0101]    A “comparison window”, as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. 
         [0102]    Methods of alignment of sequences for comparison are well-known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith &amp; Waterman, 1981, Adv. Appl. Math. 2:482, by the homology alignment algorithm of Needleman &amp; Wunsch, 1970, J. Mol. Biol. 48:443, by the search for similarity method of Pearson &amp; Lipman, 1988, Proc. Narl. Acad. Sci. USA 85:2444, by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by manual alignment and visual inspection (see, e.g., Current Protocols in Molecular Biology (Ausubel et al., eds. 1995 supplement)). 
         [0103]    A preferred example of algorithm that is suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al., 1977, Nue. Acids Res. 25:3389-3402 and Altschul et al., 1990, J. Mol. Biol. 215:403-410, respectively. BLAST and BLAST 2.0 are used, typically with the default parameters described herein, to determine percent sequence identity for the nucleic acids and proteins of the invention. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always &gt;0) and N (penalty score for mismatching residues; always &lt;0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a word length (W) of 11, an expectation (E) of 10, M=5, N=−4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a word length of 3, and expectation (E) of 10, and the BLOSUM62 scoring matrix (see  Henikoff  &amp;  Henikoff, Proc. Natl. Acad. Sci. USA  89:10915 (1989)) alignments (B) of 50, expectation (E) of 10, M=5, N=−4, and a comparison of both strands. 
         [0104]    The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin &amp; Altschul, 1993, Proc. Nat&#39;l. Acad. Sci. USA 90:5873-5787). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most preferably less than about 0.001. 
         [0105]    The phrase “stringent hybridization conditions” refers to conditions under which a probe will hybridize to its target subsequence, typically in a complex mixture of nucleic acid, but to no other sequences. Stringent conditions are sequence-dependent and will be different in different circumstances. Longer sequences hybridize specifically at higher temperatures. An extensive guide to the hybridization of nucleic acids is found in Tijssen, Techniques in Biochemistry and Molecular Biology—Hybridization with Nucleic Probes, “Overview of principles of hybridization and the strategy of nucleic acid assays” (1993). Generally, highly stringent conditions are selected to be about 5-10° C. lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength pH. Low stringency conditions are generally selected to be about 15-30° C. below the Tm. Tm is the temperature (under defined ionic strength, pH, and nucleic concentration) at which 50% of the probes complementary to the target hybridize to the target sequence at equilibrium (as the target sequences are present in excess, at Tm, 50% of the probes are occupied at equilibrium). Stringent conditions will be those in which the salt concentration is less than about 1.0M sodium ion, typically about 0.01 to 1.0M sodium ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short probes (e.g., 10 to 50 nucleotides) and at least about 60° C. for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide For selective or specific hybridization, a positive signal is at least two times background, preferably 10 times background hybridization. 
         [0106]    Nucleic acids that do not hybridize to each other under stringent conditions are still substantially identical if the polypeptides which they encode are substantially identical. This occurs, for example, when a copy of a nucleic acid is created using the maximum codon degeneracy permitted by the genetic code. In such cased, the nucleic acids typically hybridize under moderately stringent hybridization conditions. 
         [0107]    Genomic DNA or cDNA including GPT polynucleotides may be identified in standard Southern blots under stringent conditions using the GPT polynucleotide sequences disclosed here. For this purpose, suitable stringent conditions for such hybridizations are those which include a hybridization in a buffer of 40% formamide, 1M NaCl, 1% SDS at 37° C., and at least one wash in 0.2×SSC at a temperature of at least about 50° C., usually about 55° C. to about 60° C., for 20 minutes, or equivalent conditions. A positive hybridization is at least twice background. Those of ordinary skill will readily recognize that alternative hybridization and wash conditions may be utilized to provide conditions of similar stringency. 
         [0108]    A further indication that two polynucleotides are substantially identical is if the reference sequence, amplified by a pair of oligonucleotide primers, can then be used as a probe under stringent hybridization conditions to isolate the test sequence from a cDNA or genomic library, or to identify the test sequence in, e.g., a northern or Southern blot. 
       Transgenic Algae: 
       [0109]    The invention provides novel transgenic algae exhibiting faster growth and increased chlorophyll production. The transgenic algae of the invention are generated by introducing into algae cells one or more expressible genetic constructs capable of driving the expression of one or more polynucleotides encoding glutamine phenylpyruvate transaminase (GPT), and in some embodiments, one or more polynucleotides encoding glutamine synthetase (GS) and GPT. 
         [0110]    The transgenic algae of the invention may be of any species capable of transformation, including those from the subphyla  Chlorophyta, Chrysophyta, Phaeophyta, Rhodophyta , as well as the  Cyanobacteria . In the last few years, successful genetic transformation of ˜25 algal species has been demonstrated, mostly via nuclear transformation (Hallmann, 2007, Transgenic Plant Journal 1:81-98). Among these, at least ten green algae species have been successfully transformed (mostly all unicellular species). Several species of red algae, brown algae and diatom species has also been reported (Hallmann, 2007, supra). 
         [0111]    Improving the production step of algal biofuels production is being approached in more or less the standard ways of improving biological production, i.e., by optimizing nutrients, growth conditions and light/energy supply. Mineral nutrient supplies are being optimized through detailed studies of the need for each nutrient, and at what stoichiometry relative to other nutrients. The availability of carbon dioxide is being maximized typically with various delivery systems, each designed to maximize the amount of carbon dioxide dissolved in the water. As well, pH is being maintained at or near the optimum for growth. 
         [0112]    Some attempts have been made to grow algal faster and more economically by establishing heterotrophic growth conditions. This has been achieved by providing fixed carbon, such as sugars, for the algae to use as carbon and energy sources. Algae also require an optimal amount of light to provide the energy for growth. The light impinging upon an algal cell is converted to chemical energy and used to drive the algal metabolism and cell growth. The amount of light striking algal cells in growing cultures is impacted by culture density, the distance the light must penetrate into the culture (i.e., the cells at the surface receive more light than cells further from the surface), and the amount of light receptors within an algal cell. Algal bioreactor designs are being tested that range from deep or shallow horizontal ponds to vertical glass/plastic reactors. 
         [0113]    Alga cells over-expressing GPT and GS transgenes can be expected to take much better advantage of the optimized nutrients and the high availability of carbon dioxide, because they can be expected to increase their carbon dioxide fixing machinery, the ribulose bisphosphate carboxylase (RUBISCO) enzyme protein and activity state. If algae respond in the same way GPT+GS1 transgenic plants do (see co-owned, co-pending U.S. patent application Ser. No. 12/551,271), the over-expression of the GPT and GS transgenes in algae may be accompanied by increased expression of genes encoding RUBISCO subunits and the RUBISCO activase enzyme which controls the activity state of RUBISCO. The transgenic algae of the invention may also increase their capacity for and rate of uptake of nitrogen-based nutrients such as nitrate and ammonia. Such increased carbon fixation will result in increased flux through central metabolism and the incumbent increase in the concentration of organic acids that are known to induce production of the nitrogen uptake transporters. 
         [0114]    In stable transformation embodiments of the invention, one or more copies of the expressible genetic construct become integrated into the host algae genome, thereby providing increased GS and/or GPT enzyme capacity into the algae, which may serve to mediate increased synthesis of 2-oxoglutaramate therein, which in turn signals metabolic gene expression, resulting in increased algal growth. 2-oxoglutaramate is a metabolite which is an extremely potent effector of gene expression, metabolism and plant growth (U.S. Pat. No. 6,555,500), and which may play a pivotal role in the coordination of the carbon and nitrogen metabolism systems in plants (Lancien et al., 2000, Enzyme Redundancy and the Importance of 2-Oxoglutarate in  Higher Plants Ammonium Assimilation , Plant Physiol. 123:817-824). 
         [0115]    The invention also provides methods of generating transgenic algae having faster growth rates. In one embodiment, a method of generating a transgenic algae having a faster growth rate, introducing into an algal cell an expression cassette including a nucleic acid molecule encoding a GPT transgene, under the control of a suitable promoter capable of driving the expression of the transgene, so as to yield a transformed algal cell, and obtaining a transgenic algae which expresses the encoded GPT. In another embodiment, a method of generating a transgenic algae having a faster growth rate comprises introducing into an algal cell one or more nucleic acid constructs or expression cassettes including nucleic acid molecules encoding a GPT transgene and an GS transgene, under the control of one or more suitable promoters (and, optionally, other regulatory elements) capable of driving the expression of the transgenes, so as to yield an algal cell transformed thereby, and obtaining a transgenic algae which expresses the GPT and GS transgenes. 
         [0116]    GPT and GS transgenes suitable for use in generating the transgenic algae of the invention are described in co-owned, co-pending U.S. patent application Ser. No. 12/551,271. Other GPT polynucleotides suitable for use as GPT transgenes in the practice of the invention may be obtained by various means, as will be appreciated by one skilled in the art, tested for the ability to direct the expression of a GPT with GPT activity in a recombinant expression, or in a transient in planta expression system (U.S. Ser. No. 12/551,271, supra), or preferably in a transgenic algae. 
       Transgene Constructs/Algal Expression Vectors 
       [0117]    In order to generate the transgenic algae of the invention, the gene coding sequence for the desired transgene(s) must be incorporated into a nucleic acid construct (also interchangeably referred to herein as a/an (transgene) expression vector, expression cassette, expression construct or expressible genetic construct), which can direct the expression of the transgene sequence in transformed algal cells. Such nucleic acid constructs carrying the transgene(s) of interest may be introduced into an algal cell or cells using a number of methods known in the art, including but not limited to electroporation, DNA bombardment or biolistic approaches, microinjection, and via the use of various DNA-based vectors. Once introduced into the transformed algal cell, the nucleic acid construct may direct the expression of the incorporated transgene(s) (i.e., GPT), either in a transient or stable fashion. Stable expression is preferred, and is achieved by utilizing transformation vectors which are able to direct the chromosomal integration of the transgene construct. 
         [0118]    The basic elements of a nucleic acid construct for use in generating the transgenic algae of the invention are: a suitable promoter capable of directing the functional expression of the transgene(s) in a transformed algae cell, the transgene(s) (i.e., GPT coding sequence) operably linked to the promoter, preferably a suitable transcription termination sequence (i.e., nopaline synthetic enzyme gene terminator) operably linked to the transgene, and sometimes other elements useful for controlling the expression of the transgene, as well as one or more selectable marker genes suitable for selecting the desired transgenic product (i.e., antibiotic resistance genes). 
         [0119]    Various plant, algae and animal GPT protein sequences and encoding DNA and GPT transgene expression constructs are presented in the Table of Sequences, infra. Similarly, various GS1 protein sequences and encoding DNA and GS1 transgene expression constructs are provided. These sequences are provided as examples and should not be considered limiting. 
         [0120]    Typically, algae is transformed by causing the temporal permeabilization of the cell membrane, there by enabling vector DNA to enter the algal cell. DNA integration occurs naturally, by recombination events, resulting in ectopic integration into the algal genome, resulting in stable events. Biolistic approaches, such as particle bombardment, are typically used to transform algal cells. Polyethylene glycol mixtures using DNA coated particle has also been successfully utilized. Additionally, certain wall-reduced algae strains have been employed, in order to achieve protoplast transformation in the presence of polyethylene glycol and the transgene construct. Electroporation and even  Agrobacterium -mediated transformation of algae has been reported. For details, and references to published reports of various transformation protocols and vector systems, see Hallmann, 2007, supra for review. 
         [0121]    Any promoter capable of being functional in algae may be used to direct the expression of the GPT or GPT+GS transgene constructs. In preferred embodiments, the promoter may be an endogenous algal promoter. Various vectors used to transform algae are known, including plasmid vectors which become integrated into the nucleus of algal cells and there direct the cytoplasmic expression of the transgene products (i.e., plasmid pSSCR7, Davies et al., 1994, Plant Cell 6:53-63). Other nuclear-directed vectors direct transgene expression products to the periplasm. One such vector is a derivative of pSSCR7, modified to incorporate a 5′ aryl sulfatase periplasmic targeting transit sequence (Davies et al., 1994, supra). Still other vectors direct transgene integration to the chloroplast plastome by homologous recombination, whereby transgene expression is localized to the chloroplast (Hutchinson, et al., 1996, In: Molecular Genetics of Photosynthesis, Frontiers in Molecular Biology. Anderson B., Salter A H, and Barber J. eds., Oxford University Press, pp. 180-196). 
       EXAMPLES 
       [0122]    Various aspects of the invention are further described and illustrated by way of the example which follows, which is not intended to limit the scope of the invention. 
       Example 1 
     Generation of Transgenic  Chlorella    
     Materials and Methods: 
       [0123]      Chlorella vutagaris  strain #259 was purchased from Culture Collection of Algae at the University of Texas (UTEX, Austin, Tex.), and maintained as recommended on Bristol medium. 
         [0124]    Two transgene expression vectors were assembled and used to transform the algae with electroporation. The first vector was the Cambia 1201 vector containing Tomato Rubisco small subunit promoter and  Arabidopsis  GS (SEQ ID NO: 45). The second vector was the Cambia 1305.1 vector containing 35S CMV promoter  Zea mays  full length GPT gene sequence [SEQ ID NO: 46] 
         [0125]    Electrotransformation was used to insert these vectors simultaneously into the  Chlorella  culture; this was carried out according to the method of K. C. Chow and W. L. Tung. (Electrotransformation of  Chlorella vulgaris . Plant Cell Reports 1999 18:778-780). The antibiotic selection was Hygromycin B at a concentration of 20 micrograms/ml. Bristol peptone digest media (UTEX web site) was used to culture the cells on plates. 
       Results: 
       [0126]    Transformed and control algae were plated out identically. Twelve days later, 15 dark green colonies were observed growing on the transformed plate and none on the control plate.  FIG. 1  shows a photograph of the transformed (right) and untransformed (control, left)  Chlorella , taken 23 days post-plating. Vigorous green cultures were seen only on the transformed algae plate. In addition, the transformed algae colonies showed far greener and darker coloration compared the control algae. The dark green is chlorophyll, and the increase in chlorophyll per cell has been observed as a characteristic of the faster growing plants. Finally, the colony count at day 15 post-plating, and the much greater colony numbers at 23 days post-plating, are consistent with faster growth. 
         [0127]    All publications, patents, and patent applications cited in this specification are herein incorporated by reference as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. 
         [0128]    The present invention is not to be limited in scope by the embodiments disclosed herein, which are intended as single illustrations of individual aspects of the invention, and any which are functionally equivalent are within the scope of the invention. Various modifications to the models and methods of the invention, in addition to those described herein, will become apparent to those skilled in the art from the foregoing description and teachings, and are similarly intended to fall within the scope of the invention. Such modifications or other embodiments can be practiced without departing from the true scope and spirit of the invention. 
         [0000]    
       
         
               
             
               
             
           
               
                   
               
               
                 TABLE OF SEQUENCES: 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 SEQ ID NO: 1  Arabidopsis glutamine  phenylpyruvate transaminase DNA coding sequence: 
               
               
                 ATGTACCTGGACATAAATGGTGTGATGATCAAACAGTTTAGCTTCAAAGCCTCTCTT 
               
               
                 CTCCCATTCTCTTCTAATTTCCGACAAAGCTCCGCCAAAATCCATCGTCCTATCGGAG 
               
               
                 CCACCATGACCACAGTTTCGACTCAGAACGAGTCTACTCAAAAACCCGTCCAGGTG 
               
               
                 GCGAAGAGATTAGAGAAGTTCAAGACTACTATTTTCACTCAAATGAGCATATTGGCA 
               
               
                 GTTAAACATGGAGCGATCAATTTAGGCCAAGGCTTTCCCAATTTCGACGGTCCTGAT 
               
               
                 TTTGTTAAAGAAGCTGCGATCCAAGCTATTAAAGATGGTAAAAACCAGTATGCTCGT 
               
               
                 GGATACGGCATTCCTCAGCTCAACTCTGCTATAGCTGCGCGGTTTCGTGAAGATACG 
               
               
                 GGTCTTGTTGTTGATCCTGAGAAAGAAGTTACTGTTACATCTGGTTGCACAGAAGCC 
               
               
                 ATAGCTGCAGCTATGTTGGGTTTAATAAACCCTGGTGATGAAGTCATTCTCTTTGCA 
               
               
                 CCGTTTTATGATTCCTATGAAGCAACACTCTCTATGGCTGGTGCTAAAGTAAAAGGA 
               
               
                 ATCACTTTACGTCCACCGGACTTCTCCATCCCTTTGGAAGAGCTTAAAGCTGCGGTA 
               
               
                 ACTAACAAGACTCGAGCCATCCTTATGAACACTCCGCACAACCCGACCGGGAAGAT 
               
               
                 GTTCACTAGGGAGGAGCTTGAAACCATTGCATCTCTCTGCATTGAAAACGATGTGCT 
               
               
                 TGTGTTCTCGGATGAAGTATACGATAAGCTTGCGTTTGAAATGGATCACATTTCTAT 
               
               
                 AGCTTCTCTTCCCGGTATGTATGAAAGAACTGTGACCATGAATTCCCTGGGAAAGAC 
               
               
                 TTTCTCTTTAACCGGATGGAAGATCGGCTGGGCGATTGCGCCGCCTCATCTGACTTG 
               
               
                 GGGAGTTCGACAAGCACACTCTTACCTCACATTCGCCACATCAACACCAGCACAATG 
               
               
                 GGCAGCCGTTGCAGCTCTCAAGGCACCAGAGTCTTACTTCAAAGAGCTGAAAAGAG 
               
               
                 ATTACAATGTGAAAAAGGAGACTCTGGTTAAGGGTTTGAAGGAAGTCGGATTTACA 
               
               
                 GTGTTCCCATCGAGCGGGACTTACTTTGTGGTTGCTGATCACACTCCATTTGGAATG 
               
               
                 GAGAACGATGTTGCTTTCTGTGAGTATCTTATTGAAGAAGTTGGGGTCGTTGCGATC 
               
               
                 CCAACGAGCGTCTTTTATCTGAATCCAGAAGAAGGGAAGAATTTGGTTAGGTTTGCG 
               
               
                 TTCTGTAAAGACGAAGAGACGTTGCGTGGTGCAATTGAGAGGATGAAGCAGAAGCT 
               
               
                 TAAGAGAAAAGTCTGA 
               
               
                   
               
               
                 SEQ ID NO: 2  Arabidopsis  GPT amino acid sequence 
               
               
                 MYLDINGVMIKQFSFKASLLPFSSNFRQSSAKIHRPIGATMTTVSTQNESTQKPVQVAKR 
               
               
                 LEKFKTTIFTQMSILAVKHGAINLGQGFPNFDGPDFVKEAAIQAIKDGKNQYARGYGIPQ 
               
               
                 LNSAIAARFREDTGLVVDPEKEVTVTSGCTEAIAAAMLGLINPGDEVILFAPFYDSYEAT 
               
               
                 LSMAGAKVKGITLRPPDFSIPLEELKAAVTNKTRAILMNTPHNPTGKMFTREELETIASL 
               
               
                 CIENDVLVFSDEVYDKLAFEMDHISIASLPGMYERTVTMNSLGKTFSLTGWKIGWAIAPP 
               
               
                 HLTWGVRQAHSYLTFATSTPAQWAAVAALKAPESYFKELKRDYNVKKETLVKGLKEV 
               
               
                 GFTVFPSSGTYFVVADHTPFGMENDVAFCEYLIEEVGVVAIPTSVFYLNPEEGKNLVRFA 
               
               
                 FCKDEETLRGAIERMKQKLKRKV 
               
               
                   
               
               
                 SEQ ID NO: 3 Alfalfa GS1 DNA coding sequence (upper case) with 5′ and 3′ untranslated 
               
               
                 sequences (indicated in lower case). 
               
               
                 Atttccgttttcgttttcatttgattcattgaatcaaatcgaatcgaatctttaggattcaatacagattccttagattttactaagtttgaaaccaaaa 
               
               
                 ccaaaacATGTCTCTCCTTTCAGATCTTATCAACCTTGACCTCTCCGAAACCACCGAGAA 
               
               
                 AATCATCGCCGAATACATATGGATTGGTGGATCTGGTTTGGACTTGAGGAGCAAAGC 
               
               
                 AAGGACTCTACCAGGACCAGTTACTGACCCTTCACAGCTTCCCAAGTGGAACTATGA 
               
               
                 TGGTTCCAGCACAGGTCAAGCTCCTGGAGAAGATAGTGAAGTTATTATCTACCCACA 
               
               
                 AGCCATTTTCAAGGACCCATTTAGAAGGGGTAACAATATCTTGGTTATGTGTGATGC 
               
               
                 ATACACTCCAGCTGGAGAGCCCATTCCCACCAACAAGAGACATGCAGCTGCCAAGA 
               
               
                 TTTTCAGCCATCCTGATGTTGTTGCTGAAGTACCATGGTATGGTATTGAGCAAGAAT 
               
               
                 ACACCTTGTTGCAGAAAGACATCAATTGGCCTCTTGGTTGGCCAGTTGGTGGTTTTC 
               
               
                 CTGGACCTCAGGGACCATACTATTGTGGAGCTGGTGCTGACAAGGCATTTGGCCGTG 
               
               
                 ACATTGTTGACTCACATTACAAAGCCTGTCTTTATGCCGGCATCAACATCAGTGGAA 
               
               
                 TCAATGGTGAAGTGATGCCTGGTCAATGGGAATTCCAAGTTGGTCCCTCAGTTGGTA 
               
               
                 TCTCTGCTGGTGATGAGATATGGGTTGCTCGTTACATTTTGGAGAGGATCACTGAGG 
               
               
                 TTGCTGGTGTGGTGCTTTCCTTTGACCCAAAACCAATTAAGGGTGATTGGAATGGTG 
               
               
                 CTGGTGCTCACACAAATTACAGCACCAAGTCTATGAGAGAAGATGGTGGCTATGAA 
               
               
                 GTCATCTTGAAAGCAATTGAGAAGCTTGGGAAGAAGCACAAGGAGCACATTGCTGC 
               
               
                 TTATGGAGAAGGCAACGAGCGTAGATTGACAGGGCGACATGAGACAGCTGACATTA 
               
               
                 ACACCTTCTTATGGGGTGTTGCAAACCGTGGTGCGTCGATTAGAGTTGGAAGGGACA 
               
               
                 CAGAGAAAGCAGGGAAAGGTTATTTCGAGGATAGGAGGCCATCATCTAACATGGAT 
               
               
                 CCATATGTTGTTACTTCCATGATTGCAGACACCACCATTCTCTGGAAACCATAAgccac 
               
               
                 cacacacacatgcattgaagtatttgaaagtcattgttgattccgcattagaatttggtcattgttttttctaggatttggatttgtgttattgttatggtt 
               
               
                 cacactttgtttgtttgaatttgaggccttgttataggtttcatatttctttctcttgttctaagtaaatgtcagaataataatgtaat 
               
               
                   
               
               
                 SEQ ID NO: 4 Alfalfa GS1 amino acid sequence 
               
               
                 MSLLSDLINLDLSETTEKIIAEYIWIGGSGLDLRSKARTLPGPVTDPSQLPKWNYDGSSTG 
               
               
                 QAPGEDSEVIIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFSHPDVVAE 
               
               
                 VPWYGIEQEYTLLQKDINWPLGWPVGGFPGPQGPYYCGAGADKAFGRDIVDSHYKACL 
               
               
                 YAGINISGINGEVMPGQWEFQVGPSVGISAGDEIWVARYILERITEVAGVVLSFDPKPIKG 
               
               
                 DWNGAGAHTNYSTKSMREDGGYEVILKAIEKLGKKHKEHIAAYGEGNERRLTGRHETA 
               
               
                 DINTFLWGVANRGASIRVGRDTEKAGKGYFEDRRPSSNMDPYVVTSMIADTTILWKP 
               
               
                   
               
               
                 SEQ ID NO: 5 Alfalfa GS1 DNA coding sequence (upper case) with 5′ and 3′ untranslated 
               
               
                 sequences (indicated in lower case) and vector sequences from Cla1 to Sma1/Ssp1 and Ssp1/Sma1 
               
               
                 to Sal1/Xho1 (lower case, underlined). 
               
               
                   atcgatgaattcgagctcggtaccc atttccgttttcgttttcatttgattcattgaatcaaatcgaatcgaatctttaggattcaatacagattcctta 
               
               
                 gattttactaagtttgaaaccaaaaccaaaacATGTCTCTCCTTTCAGATCTTATCAACCTTGACCTCTCC 
               
               
                 GAAACCACCGAGAAAATCATCGCCGAATACATATGGATTGGTGGATCTGGTTTGGA 
               
               
                 CTTGAGGAGCAAAGCAAGGACTCTACCAGGACCAGTTACTGACCCTTCACAGCTTCC 
               
               
                 CAAGTGGAACTATGATGGTTCCAGCACAGGTCAAGCTCCTGGAGAAGATAGTGAAG 
               
               
                 TTATTATCTACCCACAAGCCATTTTCAAGGACCCATTTAGAAGGGGTAACAATATCT 
               
               
                 TGGTTATGTGTGATGCATACACTCCAGCTGGAGAGCCCATTCCCACCAACAAGAGAC 
               
               
                 ATGCAGCTGCCAAGATTTTCAGCCATCCTGATGTTGTTGCTGAAGTACCATGGTATG 
               
               
                 GTATTGAGCAAGAATACACCTTGTTGCAGAAAGACATCAATTGGCCTCTTGGTTGGC 
               
               
                 CAGTTGGTGGTTTTCCTGGACCTCAGGGACCATACTATTGTGGAGCTGGTGCTGACA 
               
               
                 AGGCATTTGGCCGTGACATTGTTGACTCACATTACAAAGCCTGTCTTTATGCCGGCA 
               
               
                 TCAACATCAGTGGAATCAATGGTGAAGTGATGCCTGGTCAATGGGAATTCCAAGTTG 
               
               
                 GTCCCTCAGTTGGTATCTCTGCTGGTGATGAGATATGGGTTGCTCGTTACATTTTGGA 
               
               
                 GAGGATCACTGAGGTTGCTGGTGTGGTGCTTTCCTTTGACCCAAAACCAATTAAGGG 
               
               
                 TGATTGGAATGGTGCTGGTGCTCACACAAATTACAGCACCAAGTCTATGAGAGAAG 
               
               
                 ATGGTGGCTATGAAGTCATCTTGAAAGCAATTGAGAAGCTTGGGAAGAAGCACAAG 
               
               
                 GAGCACATTGCTGCTTATGGAGAAGGCAACGAGCGTAGATTGACAGGGCGACATGA 
               
               
                 GACAGCTGACATTAACACCTTCTTATGGGGTGTTGCAAACCGTGGTGCGTCGATTAG 
               
               
                 AGTTGGAAGGGACACAGAGAAAGCAGGGAAAGGTTATTTCGAGGATAGGAGGCCA 
               
               
                 TCATCTAACATGGATCCATATGTTGTTACTTCCATGATTGCAGACACCACCATTCTCT 
               
               
                 GGAAACCATAAgccaccacacacacatgcattgaagtatttgaaagtcattgttgattccgcattagaatttggtcattgttttttctag 
               
               
                 gatttggatttgtgttattgttatggttcacactttgtttgtttgaatttgaggccttgttataggtttcatatttctttctcttgttctaagtaaatgtcaga 
               
               
                 ataataatgtaatggggatcctctagagtcgag 
               
               
                   
               
               
                 SEQ ID NO: 6  Arabidopsis  GS1 coding sequence 
               
               
                 Cambia 1201 vector + rbcS3C +  arabidopsis  GS1Bold  ATG  is the start site, 
               
               
                 AAAAAAGAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGAGATAAGGA 
               
               
                 CGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGAACCACAAA 
               
               
                 ATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTCCGTTAGAT 
               
               
                 AGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTAACCAATTA 
               
               
                 TTTCAGC          TCTCTGCTCTCAGATCTCGTTAACCTCAACCTCACCGATGCCACC 
               
               
                 GGGAAAATCATCGCCGAATACATATGGATCGGTGGATCTGGAATGGATATCAGAAG 
               
               
                 CAAAGCCAGGACACTACCAGGACCAGTGACTGATCCATCAAAGCTTCCCAAGTGGA 
               
               
                 ACTACGACGGATCCAGCACCGGTCAGGCTGCTGGAGAAGACAGTGAAGTCATTCTA 
               
               
                 TACCCTCAGGCAATATTCAAGGATCCCTTCAGGAAAGGCAACAACATCCTGGTGATG 
               
               
                 TGTGATGCTTACACACCAGCTGGTGATCCTATTCCAACCAACAAGAGGCACAACGCT 
               
               
                 GCTAAGATCTTCAGCCACCCCGACGTTGCCAAGGAGGAGCCTTGGTATGGGATTGA 
               
               
                 GCAAGAATACACTTTGATGCAAAAGGATGTGAACTGGCCAATTGGTTGGCCTGTTGG 
               
               
                 TGGCTACCCTGGCCCTCAGGGACCTTACTACTGTGGTGTGGGAGCTGACAAAGCCAT 
               
               
                 TGGTCGTGACATTGTGGATGCTCACTACAAGGCCTGTCTTTACGCCGGTATTGGTATT 
               
               
                 TCTGGTATCAATGGAGAAGTCATGCCAGGCCAGTGGGAGTTCCAAGTCGGCCCTGTT 
               
               
                 GAGGGTATTAGTTCTGGTGATCAAGTCTGGGTTGCTCGATACCTTCTCGAGAGGATC 
               
               
                 ACTGAGATCTCTGGTGTAATTGTCAGCTTCGACCCGAAACCAGTCCCGGGTGACTGG 
               
               
                 AATGGAGCTGGAGCTCACTGCAACTACAGCACTAAGACAATGAGAAACGATGGAGG 
               
               
                 ATTAGAAGTGATCAAGAAAGCGATAGGGAAGCTTCAGCTGAAACACAAAGAACAC 
               
               
                 ATTGCTGCTTACGGTGAAGGAAACGAGCGTCGTCTCACTGGAAAGCACGAAACCGC 
               
               
                 AGACATCAACACATTCTCTTGGGGAGTCGCGAACCGTGGAGCGTCAGTGAGAGTGG 
               
               
                 GACGTGACACAGAGAAGGAAGGTAAAGGGTACTTCGAAGACAGAAGGCCAGCTTCT 
               
               
                 AACATGGATCCTTACGTTGTCACCTCCATGATCGCTGAGACGACCATACTCGGTTGA 
               
               
                   
               
               
                 SEQ ID NO: 7  Arabidopsis  GS1 amino acid sequence 
               
               
                 Vector sequences at N-terminus in italics 
               
               
                   MVDLRNRRTS MSLLSDLVNLNLTDATGKIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLP 
               
               
                 KWNYDGSSTGQAAGEDSEVILYPQAIFKDPFRKGNNILVMCDAYTPAGDPIPTNKRHNA 
               
               
                 AKIFSHPDVAKEEPWYGIEQEYTLMQKDVNWPIGWPVGGYPGPQGPYYCGVGADKAIG 
               
               
                 RDIVDAHYKACLYAGIGISGINGEVMPGQWEFQVGPVEGISSGDQVWVARYLLERITEIS 
               
               
                 GVIVSFDPKPVPGDWNGAGAHCNYSTKTMRNDGGLEVIKKAIGKLQLKHKEHIAAYGE 
               
               
                 GNERRLTGKHETADINTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNMDPYVV 
               
               
                 TSMIAETTILG 
               
               
                   
               
               
                 SEQ ID NO: 8 Grape GPT coding DNA sequence 
               
               
                 Showing Cambia 1305.1 with (3′ end of) rbcS3C +  Vitis vinifera  GPT (Grape). 
               
               
                 Bold  ATG  is the start site, parentheses are the cat1 intron and the underlined 
               
               
                 actagt is the spe1 cloning site used to splice in the GPT gene. 
               
               
                 AAAAAAGAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGAGATAAGGA 
               
               
                 CGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGAACCACAAA 
               
               
                 ATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTCCGTTAGAT 
               
               
                 AGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTAACCAATTA 
               
               
                 TTTCAGCA          TAGATCTGAGG(GTAAATTTCTAGTTTTTCTCCTTCATTTTCTTG 
               
               
                 GTTAGGACCCTTTTCTCTTTTTATTTTTTTGAGCTTTGATCTTTCTTTAAACTGATCTA 
               
               
                 TTTTTTAATTGATTGGTTATGGTGTAAATATTACATAGCTTTAACTGATAATCTGATT 
               
               
                 ACTTTATTTCGTGTGTCTATGATGATGATGATAGTTACAG)AACCGACGA          AT 
               
               
                 GCAGCTCTCTCAATGTACCTGGACATTCCCAGAGTTGCTTAAAAGACCAGCCTTTTT 
               
               
                 AAGGAGGAGTATTGATAGTATTTCGAGTAGAAGTAGGTCCAGCTCCAAGTATCCATC 
               
               
                 TTTCATGGCGTCCGCATCAACGGTCTCCGCTCCAAATACGGAGGCTGAGCAGACCCA 
               
               
                 TAACCCCCCTCAACCTCTACAGGTTGCAAAGCGCTTGGAGAAATTCAAAACAACAA 
               
               
                 TCTTTACTCAAATGAGCATGCTTGCCATCAAACATGGAGCAATAAACCTTGGCCAAG 
               
               
                 GGTTTCCCAACTTTGATGGTCCTGAGTTTGTCAAAGAAGCAGCAATTCAAGCCATTA 
               
               
                 AGGATGGGAAAAACCAATATGCTCGTGGATATGGAGTTCCTGATCTCAACTCTGCTG 
               
               
                 TTGCTGATAGATTCAAGAAGGATACAGGACTCGTGGTGGACCCCGAGAAGGAAGTT 
               
               
                 ACTGTTACTTCTGGATGTACAGAAGCAATTGCTGCTACTATGCTAGGCTTGATAAAT 
               
               
                 CCTGGTGATGAGGTGATCCTCTTTGCTCCATTTTATGATTCCTATGAAGCCACTCTAT 
               
               
                 CCATGGCTGGTGCCCAAATAAAATCCATCACTTTACGTCCTCCGGATTTTGCTGTGCC 
               
               
                 CATGGATGAGCTCAAGTCTGCAATCTCAAAGAATACCCGTGCAATCCTTATAAACAC 
               
               
                 TCCCCATAACCCCACAGGAAAGATGTTCACAAGGGAGGAACTGAATGTGATTGCAT 
               
               
                 CCCTCTGCATTGAGAATGATGTGTTGGTGTTTACTGATGAAGTTTACGACAAGTTGG 
               
               
                 CTTTCGAAATGGATCACATTTCCATGGCTTCTCTTCCTGGGATGTACGAGAGGACCG 
               
               
                 TGACTATGAATTCCTTAGGGAAAACTTTCTCCCTGACTGGATGGAAGATTGGTTGGA 
               
               
                 CAGTAGCTCCCCCACACCTGACATGGGGAGTGAGGCAAGCCCACTCATTCCTCACGT 
               
               
                 TTGCTACCTGCACCCCAATGCAATGGGCAGCTGCAACAGCCCTCCGGGCCCCAGACT 
               
               
                 CTTACTATGAAGAGCTAAAGAGAGATTACAGTGCAAAGAAGGCAATCCTGGTGGAG 
               
               
                 GGATTGAAGGCTGTCGGTTTCAGGGTATACCCATCAAGTGGGACCTATTTTGTGGTG 
               
               
                 GTGGATCACACCCCATTTGGGTTGAAAGACGATATTGCGTTTTGTGAGTATCTGATC 
               
               
                 AAGGAAGTTGGGGTGGTAGCAATTCCGACAAGCGTTTTCTACTTACACCCAGAAGAT 
               
               
                 GGAAAGAACCTTGTGAGGTTTACCTTCTGTAAAGACGAGGGAACTCTGAGAGCTGC 
               
               
                 AGTTGAAAGGATGAAGGAGAAACTGAAGCCTAAACAATAGGGGCACGTGA 
               
               
                   
               
               
                 SEQ ID NO: 9 Grape GPT amino acid sequence 
               
               
                 MVDLRNRRTSMQLSQCTWTFPELLKRPAFLRRSIDSISSRSRSSSKYPSFMASASTVSAPN 
               
               
                 TEAEQTHNPPQPLQVAKRLEKFKTTIFTQMSMLAIKHGAINLGQGFPNFDGPEFVKEAAI 
               
               
                 QAIKDGKNQYARGYGVPDLNSAVADRFKKDTGLVVDPEKEVTVTSGCTEAIAATMLGL 
               
               
                 INPGDEVILFAPFYDSYEATLSMAGAQIKSITLRPPDFAVPMDELKSAISKNTRAILINTPH 
               
               
                 NPTGKMFTREELNVIASLCIENDVLVFTDEVYDKLAFEMDHISMASLPGMYERTVTMNS 
               
               
                 LGKTFSLTGWKIGWTVAPPHLTWGVRQAHSFLTFATCTPMQWAAATALRAPDSYYEEL 
               
               
                 KRDYSAKKAILVEGLKAVGFRVYPSSGTYFVVVDHTPFGLKDDIAFCEYLIKEVGVVAIP 
               
               
                 TSVFYLHPEDGKNLVRFTFCKDEGTLRAAVERMKEKLKPKQ 
               
               
                   
               
               
                 SEQ ID NO: 10 Rice GPT DNA coding sequence 
               
               
                 Rice GPT codon optimized for  E. coli  expression; untranslated sequences shown in lower case 
               
               
                 atgtggATGAACCTGGCAGGCTTTCTGGCAACCCCGGCAACCGCAACCGCAACCCGTCA 
               
               
                 TGAAATGCCGCTGAACCCGAGCAGCAGCGCGAGCTTTCTGCTGAGCAGCCTGCGTC 
               
               
                 GTAGCCTGGTGGCGAGCCTGCGTAAAGCGAGCCCGGCAGCAGCAGCAGCACTGAGC 
               
               
                 CCGATGGCAAGCGCAAGCACCGTGGCAGCAGAAAACGGTGCAGCAAAAGCAGCAG 
               
               
                 CAGAAAAACAGCAGCAGCAGCCGGTGCAGGTGGCGAAACGTCTGGAAAAATTTAA 
               
               
                 AACCACCATTTTTACCCAGATGAGCATGCTGGCGATTAAACATGGCGCGATTAACCT 
               
               
                 GGGCCAGGGCTTTCCGAACTTTGATGGCCCGGATTTTGTGAAAGAAGCGGCGATTCA 
               
               
                 GGCGATTAACGCGGGCAAAAACCAGTATGCGCGTGGCTATGGCGTGCCGGAACTGA 
               
               
                 ACAGCGCGATTGCGGAACGTTTTCTGAAAGATAGCGGCCTGCAGGTGGATCCGGAA 
               
               
                 AAAGAAGTGACCGTGACCAGCGGCTGCACCGAAGCGATTGCGGCGACCATTCTGGG 
               
               
                 CCTGATTAACCCGGGCGATGAAGTGATTCTGTTTGCGCCGTTTTATGATAGCTATGA 
               
               
                 AGCGACCCTGAGCATGGCGGGCGCGAACGTGAAAGCGATTACCCTGCGTCCGCCGG 
               
               
                 ATTTTAGCGTGCCGCTGGAAGAACTGAAAGCGGCCGTGAGCAAAAACACCCGTGCG 
               
               
                 ATTATGATTAACACCCCGCATAACCCGACCGGCAAAATGTTTACCCGTGAAGAACTG 
               
               
                 GAATTTATTGCGACCCTGTGCAAAGAAAACGATGTGCTGCTGTTTGCGGATGAAGTG 
               
               
                 TATGATAAACTGGCGTTTGAAGCGGATCATATTAGCATGGCGAGCATTCCGGGCATG 
               
               
                 TATGAACGTACCGTGACCATGAACAGCCTGGGCAAAACCTTTAGCCTGACCGGCTG 
               
               
                 GAAAATTGGCTGGGCGATTGCGCCGCCGCATCTGACCTGGGGCGTGCGTCAGGCAC 
               
               
                 ATAGCTTTCTGACCTTTGCAACCTGCACCCCGATGCAGGCAGCCGCCGCAGCAGCAC 
               
               
                 TGCGTGCACCGGATAGCTATTATGAAGAACTGCGTCGTGATTATGGCGCGAAAAAA 
               
               
                 GCGCTGCTGGTGAACGGCCTGAAAGATGCGGGCTTTATTGTGTATCCGAGCAGCGGC 
               
               
                 ACCTATTTTGTGATGGTGGATCATACCCCGTTTGGCTTTGATAACGATATTGAATTTT 
               
               
                 GCGAATATCTGATTCGTGAAGTGGGCGTGGTGGCGATTCCGCCGAGCGTGTTTTATC 
               
               
                 TGAACCCGGAAGATGGCAAAAACCTGGTGCGTTTTACCTTTTGCAAAGATGATGAA 
               
               
                 ACCCTGCGTGCGGCGGTGGAACGTATGAAAACCAAACTGCGTAAAAAAAAGCTTgcg 
               
               
                 gccgcactcgagcaccaccaccaccaccactga 
               
               
                   
               
               
                 SEQ ID NO: 11 Rice GPT amino acid sequence 
               
               
                 Includes amino terminal amino acids MWfor cloning and His tag sequences from pet28 vector in 
               
               
                 italics. 
               
               
                   MW MNLAGFLATPATATATRHEMPLNPSSSASFLLSSLRRSLVASLRKASPAAAAALSPM 
               
               
                 ASASTVAAENGAAKAAAEKQQQQPVQVAKRLEKFKTTIFTQMSMLAIKHGAINLGQGF 
               
               
                 PNFDGPDFVKEAAIQAINAGKNQYARGYGVPELNSAIAERFLKDSGLQVDPEKEVTVTS 
               
               
                 GCTEAIAATILGLINPGDEVILFAPFYDSYEATLSMAGANVKAITLRPPDFSVPLEELKAA 
               
               
                 VSKNTRAIMINTPHNPTGKMFTREELEFIATLCKENDVLLFADEVYDKLAFEADHISMAS 
               
               
                 IPGMYERTVTMNSLGKTFSLTGWKIGWAIAPPHLTWGVRQAHSFLTFATCTPMQAAAA 
               
               
                 AALRAPDSYYEELRRDYGAKKALLVNGLKDAGFIVYPSSGTYFVMVDHTPFGFDNDIEF 
               
               
                 CEYLIREVGVVAIPPSVFYLNPEDGKNLVRFTFCKDDETLRAAVERMKTKLRKK KLAAA   
               
               
                 
                   LEHHHHHH 
                 
               
               
                   
               
               
                 SEQ ID NO: 12 Soybean GPT DNA coding sequence 
               
               
                 TOPO 151D WITH SOYBEAN for  E. coli  expression 
               
               
                 From starting codon. Vector sequences are italicized 
               
               
                   ATG CATCATCACCATCACCATGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGAT 
               
               
                 TCTACGGAAAACCTGTATTTTCAGGGAATTGATCCCTTCACCGCGAAACGTCTGGAA 
               
               
                 AAATTTCAGACCACCATTTTTACCCAGATGAGCCTGCTGGCGATTAAACATGGCGCG 
               
               
                 ATTAACCTGGGCCAGGGCTTTCCGAACTTTGATGGCCCGGAATTTGTGAAAGAAGCG 
               
               
                 GCGATTCAGGCGATTCGTGATGGCAAAAACCAGTATGCGCGTGGCTATGGCGTGCC 
               
               
                 GGATCTGAACATTGCGATTGCGGAACGTTTTAAAAAAGATACCGGCCTGGTGGTGG 
               
               
                 ATCCGGAAAAAGAAATTACCGTGACCAGCGGCTGCACCGAAGCGATTGCGGCGACC 
               
               
                 ATGATTGGCCTGATTAACCCGGGCGATGAAGTGATTATGTTTGCGCCGTTTTATGAT 
               
               
                 AGCTATGAAGCGACCCTGAGCATGGCGGGCGCGAAAGTGAAAGGCATTACCCTGCG 
               
               
                 TCCGCCGGATTTTGCGGTGCCGCTGGAAGAACTGAAAAGCACCATTAGCAAAAACA 
               
               
                 CCCGTGCGATTCTGATTAACACCCCGCATAACCCGACCGGCAAAATGTTTACCCGTG 
               
               
                 AAGAACTGAACTGCATTGCGAGCCTGTGCATTGAAAACGATGTGCTGGTGTTTACCG 
               
               
                 ATGAAGTGTATGATAAACTGGCGTTTGATATGGAACATATTAGCATGGCGAGCCTGC 
               
               
                 CGGGCATGTTTGAACGTACCGTGACCCTGAACAGCCTGGGCAAAACCTTTAGCCTGA 
               
               
                 CCGGCTGGAAAATTGGCTGGGCGATTGCGCCGCCGCATCTGAGCTGGGGCGTGCGT 
               
               
                 CAGGCGCATGCGTTTCTGACCTTTGCAACCGCACATCCGTTTCAGTGCGCAGCAGCA 
               
               
                 GCAGCACTGCGTGCACCGGATAGCTATTATGTGGAACTGAAACGTGATTATATGGCG 
               
               
                 AAACGTGCGATTCTGATTGAAGGCCTGAAAGCGGTGGGCTTTAAAGTGTTTCCGAGC 
               
               
                 AGCGGCACCTATTTTGTGGTGGTGGATCATACCCCGTTTGGCCTGGAAAACGATGTG 
               
               
                 GCGTTTTGCGAATATCTGGTGAAAGAAGTGGGCGTGGTGGCGATTCCGACCAGCGT 
               
               
                 GTTTTATCTGAACCCGGAAGAAGGCAAAAACCTGGTGCGTTTTACCTTTTGCAAAGA 
               
               
                 TGAAGAAACCATTCGTAGCGCGGTGGAACGTATGAAAGCGAAACTGCGTAAAGTCG 
               
               
                 ACTAA 
               
               
                   
               
               
                 SEQ ID NO: 13 Soybean GPT amino acid sequence 
               
               
                 Translated protein product, vector sequences italicized 
               
               
                   MHHHHHHGKPIPNPLLGLDSTENLYFQGIDPFT AKRLEKFQTTIFTQMSLLAIKHGAINLG 
               
               
                 QGFPNFDGPEFVKEAAIQAIRDGKNQYARGYGVPDLNIAIAERFKKDTGLVVDPEKEITV 
               
               
                 TSGCTEAIAATMIGLINPGDEVIMFAPFYDSYEATLSMAGAKVKGITLRPPDFAVPLEEL 
               
               
                 KSTISKNTRAILINTPHNPTGKMFTREELNCIASLCIENDVLVFTDEVYDKLAFDMEHISM 
               
               
                 ASLPGMFERTVTLNSLGKTFSLTGWKIGWAIAPPHLSWGVRQAHAFLTFATAHPFQCAA 
               
               
                 AAALRAPDSYYVELKRDYMAKRAILIEGLKAVGFKVFPSSGTYFVVVDHTPFGLENDV 
               
               
                 AFCEYLVKEVGVVAIPTSVFYLNPEEGKNLVRFTFCKDEETIRSAVERMKAKLRKVD 
               
               
                   
               
               
                 SEQ ID NO: 14 Barley GPT DNA coding sequence 
               
               
                 Coding sequence from start with intron removed 
               
               
                     ATG     G TAGATCTGAGGAACCGACGA          TATGGCATCCGCCCCCGCCTCCGCCTCC 
               
               
                 GCGGCCCTCTCCACCGCCGCCCCCGCCGACAACGGGGCCGCCAAGCCCACGGAGCA 
               
               
                 GCGGCCGGTACAGGTGGCTAAGCGATTGGAGAAGTTCAAAACAACAATTTTCACAC 
               
               
                 AGATGAGCATGCTCGCAGTGAAGCATGGAGCAATAAACCTTGGACAGGGGTTTCCC 
               
               
                 AATTTTGATGGCCCTGACTTTGTCAAAGATGCTGCTATTGAGGCTATCAAAGCTGGA 
               
               
                 AAGAATCAGTATGCAAGAGGATATGGTGTGCCTGAATTGAACTCAGCTGTTGCTGA 
               
               
                 GAGATTTCTCAAGGACAGTGGATTGCACATCGATCCTGATAAGGAAGTTACTGTTAC 
               
               
                 ATCTGGGTGCACAGAAGCAATAGCTGCAACGATATTGGGTCTGATCAACCCTGGGG 
               
               
                 ATGAAGTCATACTGTTTGCTCCATTCTATGATTCTTATGAGGCTACACTGTCCATGGC 
               
               
                 TGGTGCGAATGTCAAAGCCATTACACTCCGCCCTCCGGACTTTGCAGTCCCTCTTGA 
               
               
                 AGAGCTAAAGGCTGCAGTCTCGAAGAATACCAGAGCAATAATGATTAATACACCTC 
               
               
                 ACAACCCTACCGGGAAAATGTTCACAAGGGAGGAACTTGAGTTCATTGCTGATCTCT 
               
               
                 GCAAGGAAAATGACGTGTTGCTCTTTGCCGATGAGGTCTACGACAAGCTGGCGTTTG 
               
               
                 AGGCGGATCACATATCAATGGCTTCTATTCCTGGCATGTATGAGAGGACCGTCACTA 
               
               
                 TGAACTCCCTGGGGAAGACGTTCTCCTTGACCGGATGGAAGATCGGCTGGGCGATA 
               
               
                 GCACCACCGCACCTGACATGGGGCGTAAGGCAGGCACACTCCTTCCTCACATTCGCC 
               
               
                 ACCTCCACGCCGATGCAATCAGCAGCGGCGGCGGCCCTGAGAGCACCGGACAGCTA 
               
               
                 CTTTGAGGAGCTGAAGAGGGACTACGGCGCAAAGAAAGCGCTGCTGGTGGACGGGC 
               
               
                 TCAAGGCGGCGGGCTTCATCGTCTACCCTTCGAGCGGAACCTACTTCATCATGGTCG 
               
               
                 ACCACACCCCGTTCGGGTTCGACAACGACGTCGAGTTCTGCGAGTACTTGATCCGCG 
               
               
                 AGGTCGGCGTCGTGGCCATCCCGCCAAGCGTGTTCTACCTGAACCCGGAGGACGGG 
               
               
                 AAGAACCTGGTGAGGTTCACCTTCTGCAAGGACGACGACACGCTAAGGGCGGCGGT 
               
               
                 GGACAGGATGAAGGCCAAGCTCAGGAAGAAATGA 
               
               
                   
               
               
                 SEQ ID NO: 15 Barley GPT amino acid sequence 
               
               
                 Translated sequence from start site (intron removed) 
               
               
                 MVDLRNRRTSMASAPASASAALSTAAPADNGAAKPTEQRPVQVAKRLEKFKTTIFTQM 
               
               
                 SMLAVKHGAINLGQGFPNFDGPDFVKDAAIEAIKAGKNQYARGYGVPELNSAVAERFL 
               
               
                 KDSGLHIDPDKEVTVTSGCTEAIAATILGLINPGDEVILFAPFYDSYEATLSMAGANVKAI 
               
               
                 TLRPPDFAVPLEELKAAVSKNTRAIMINTPHNPTGKMFTREELEFIADLCKENDVLLFAD 
               
               
                 EVYDKLAFEADHISMASIPGMYERTVTMNSLGKTFSLTGWKIGWAIAPPHLTWGVRQA 
               
               
                 HSFLTFATSTPMQSAAAAALRAPDSYFEELKRDYGAKKALLVDGLKAAGFIVYPSSGTY 
               
               
                 FIMVDHTPFGFDNDVEFCEYLIREVGVVAIPPSVFYLNPEDGKNLVRFTFCKDDDTLRAA 
               
               
                 VDRMKAKLRKK 
               
               
                   
               
               
                 SEQ ID NO: 16 Zebra fish GPT DNA coding sequence 
               
               
                   Danio renio  sequence designed for expression in  E coli.  Bold, italicized 
               
               
                 nucleotides added for cloning or from pET28b vector. 
               
               
                            GTGGCGAAACGTCTGGAAAAATTTAAAACCACCATTTTTACCCAGATGAGC 
               
               
                 ATGCTGGCGATTAAACATGGCGCGATTAACCTGGGCCAGGGCTTTCCGAACTTTGAT 
               
               
                 GGCCCGGATTTTGTGAAAGAAGCGGCGATTCAGGCGATTCGTGATGGCAACAACCA 
               
               
                 GTATGCGCGTGGCTATGGCGTGCCGGATCTGAACATTGCGATTAGCGAACGTTATAA 
               
               
                 AAAAGATACCGGCCTGGCGGTGGATCCGGAAAAAGAAATTACCGTGACCAGCGGCT 
               
               
                 GCACCGAAGCGATTGCGGCGACCGTGCTGGGCCTGATTAACCCGGGCGATGAAGTG 
               
               
                 ATTGTGTTTGCGCCGTTTTATGATAGCTATGAAGCGACCCTGAGCATGGCGGGCGCG 
               
               
                 AAAGTGAAAGGCATTACCCTGCGTCCGCCGGATTTTGCGCTGCCGATTGAAGAACTG 
               
               
                 AAAAGCACCATTAGCAAAAACACCCGTGCGATTCTGCTGAACACCCCGCATAACCC 
               
               
                 GACCGGCAAAATGTTTACCCCGGAAGAACTGAACACCATTGCGAGCCTGTGCATTG 
               
               
                 AAAACGATGTGCTGGTGTTTAGCGATGAAGTGTATGATAAACTGGCGTTTGATATGG 
               
               
                 AACATATTAGCATTGCGAGCCTGCCGGGCATGTTTGAACGTACCGTGACCATGAACA 
               
               
                 GCCTGGGCAAAACCTTTAGCCTGACCGGCTGGAAAATTGGCTGGGCGATTGCGCCG 
               
               
                 CCGCATCTGACCTGGGGCGTGCGTCAGGCGCATGCGTTTCTGACCTTTGCAACCAGC 
               
               
                 AACCCGATGCAGTGGGCAGCAGCAGTGGCACTGCGTGCACCGGATAGCTATTATAC 
               
               
                 CGAACTGAAACGTGATTATATGGCGAAACGTAGCATTCTGGTGGAAGGCCTGAAAG 
               
               
                 CGGTGGGCTTTAAAGTGTTTCCGAGCAGCGGCACCTATTTTGTGGTGGTGGATCATA 
               
               
                 CCCCGTTTGGCCATGAAAACGATATTGCGTTTTGCGAATATCTGGTGAAAGAAGTGG 
               
               
                 GCGTGGTGGCGATTCCGACCAGCGTGTTTTATCTGAACCCGGAAGAAGGCAAAAAC 
               
               
                 CTGGTGCGTTTTACCTTTTGCAAAGATGAAGGCACCCTGCGTGCGGCGGTGGATCGT 
               
               
                 ATGAAAGAAAAACTGCGTAAAGTCGACAA                     
               
               
                 
                           
                 
               
               
                   
               
               
                 SEQ ID NO: 17 Zebra fish GPT amino acid sequence 
               
               
                 Amino acid sequence of  Danio renio  cloned and expressed in  E. coli  (bold, 
               
               
                 italicized amino acids are added from vector/cloning and His tag on C-terminus) 
               
               
                            VAKRLEKFKTTIFTQMSMLAIKHGAINLGQGFPNFDGPDFVKEAAIQAIRDGNNQYA 
               
               
                 RGYGVPDLNIAISERYKKDTGLAVDPEKEITVTSGCTEAIAATVLGLINPGDEVIVFAPFY 
               
               
                 DSYEATLSMAGAKVKGITLRPPDFALPIEELKSTISKNTRAILLNTPHNPTGKMFTPEELN 
               
               
                 TIASLCIENDVLVFSDEVYDKLAFDMEHISIASLPGMFERTVTMNSLGKTFSLTGWKIGW 
               
               
                 AIAPPHLTWGVRQAHAFLTFATSNPMQWAAAVALRAPDSYYTELKRDYMAKRSILVEG 
               
               
                 LKAVGFKVFPSSGTYFVVVDHTPFGHENDIAFCEYLVKEVGVVAIPTSVFYLNPEEGKN 
               
               
                 LVRFTFCKDEGTLRAAVDRMKEKLRK           
               
               
                   
               
               
                 SEQ ID NO: 18  Arabidopsis  truncated GPT −30 construct DNA sequence 
               
               
                   Arabidopsis  GPT coding sequence with 30 amino acids removed from the targeting sequence. 
               
               
                 ATGGCCAAAATCCATCGTCCTATCGGAGCCACCATGACCACAGTTTCGACTCAGAAC 
               
               
                 GAGTCTACTCAAAAACCCGTCCAGGTGGCGAAGAGATTAGAGAAGTTCAAGACTAC 
               
               
                 TATTTTCACTCAAATGAGCATATTGGCAGTTAAACATGGAGCGATCAATTTAGGCCA 
               
               
                 AGGCTTTCCCAATTTCGACGGTCCTGATTTTGTTAAAGAAGCTGCGATCCAAGCTAT 
               
               
                 TAAAGATGGTAAAAACCAGTATGCTCGTGGATACGGCATTCCTCAGCTCAACTCTGC 
               
               
                 TATAGCTGCGCGGTTTCGTGAAGATACGGGTCTTGTTGTTGATCCTGAGAAAGAAGT 
               
               
                 TACTGTTACATCTGGTTGCACAGAAGCCATAGCTGCAGCTATGTTGGGTTTAATAAA 
               
               
                 CCCTGGTGATGAAGTCATTCTCTTTGCACCGTTTTATGATTCCTATGAAGCAACACTC 
               
               
                 TCTATGGCTGGTGCTAAAGTAAAAGGAATCACTTTACGTCCACCGGACTTCTCCATC 
               
               
                 CCTTTGGAAGAGCTTAAAGCTGCGGTAACTAACAAGACTCGAGCCATCCTTATGAAC 
               
               
                 ACTCCGCACAACCCGACCGGGAAGATGTTCACTAGGGAGGAGCTTGAAACCATTGC 
               
               
                 ATCTCTCTGCATTGAAAACGATGTGCTTGTGTTCTCGGATGAAGTATACGATAAGCT 
               
               
                 TGCGTTTGAAATGGATCACATTTCTATAGCTTCTCTTCCCGGTATGTATGAAAGAACT 
               
               
                 GTGACCATGAATTCCCTGGGAAAGACTTTCTCTTTAACCGGATGGAAGATCGGCTGG 
               
               
                 GCGATTGCGCCGCCTCATCTGACTTGGGGAGTTCGACAAGCACACTCTTACCTCACA 
               
               
                 TTCGCCACATCAACACCAGCACAATGGGCAGCCGTTGCAGCTCTCAAGGCACCAGA 
               
               
                 GTCTTACTTCAAAGAGCTGAAAAGAGATTACAATGTGAAAAAGGAGACTCTGGTTA 
               
               
                 AGGGTTTGAAGGAAGTCGGATTTACAGTGTTCCCATCGAGCGGGACTTACTTTGTGG 
               
               
                 TTGCTGATCACACTCCATTTGGAATGGAGAACGATGTTGCTTTCTGTGAGTATCTTAT 
               
               
                 TGAAGAAGTTGGGGTCGTTGCGATCCCAACGAGCGTCTTTTATCTGAATCCAGAAGA 
               
               
                 AGGGAAGAATTTGGTTAGGTTTGCGTTCTGTAAAGACGAAGAGACGTTGCGTGGTG 
               
               
                 CAATTGAGAGGATGAAGCAGAAGCTTAAGAGAAAAGTCTGA 
               
               
                   
               
               
                 SEQ ID NO: 19  Arabidopsis  truncated GPT −30 construct amino acid sequence 
               
               
                 MAKIHRPIGATMTTVSTQNESTQKPVQVAKRLEKFKTTIFTQMSILAVKHGAINLGQGFP 
               
               
                 NFDGPDFVKEAAIQAIKDGKNQYARGYGIPQLNSAIAARFREDTGLVVDPEKEVTVTSG 
               
               
                 CTEAIAAAMLGLINPGDEVILFAPFYDSYEATLSMAGAKVKGITLRPPDFSIPLEELKAAV 
               
               
                 TNKTRAILMNTPHNPTGKMFTREELETIASLCIENDVLVFSDEVYDKLAFEMDHISIASLP 
               
               
                 GMYERTVTMNSLGKTFSLTGWKIGWAIAPPHLTWGVRQAHSYLTFATSTPAQWAAVA 
               
               
                 ALKAPESYFKELKRDYNVKKETLVKGLKEVGFTVFPSSGTYFVVADHTPFGMENDVAF 
               
               
                 CEYLIEEVGVVAIPTSVFYLNPEEGKNLVRFAFCKDEETLRGAIERMKQKLKRKV 
               
               
                   
               
               
                 SEQ ID NO: 20:  Arabidopsis  truncated GPT −45 construct DNA sequence 
               
               
                   Arabidopsis  GPT coding sequence with 45 residues in the targeting sequence removed 
               
               
                 ATGGCGACTCAGAACGAGTCTACTCAAAAACCCGTCCAGGTGGCGAAGAGATTAGA 
               
               
                 GAAGTTCAAGACTACTATTTTCACTCAAATGAGCATATTGGCAGTTAAACATGGAGC 
               
               
                 GATCAATTTAGGCCAAGGCTTTCCCAATTTCGACGGTCCTGATTTTGTTAAAGAAGC 
               
               
                 TGCGATCCAAGCTATTAAAGATGGTAAAAACCAGTATGCTCGTGGATACGGCATTCC 
               
               
                 TCAGCTCAACTCTGCTATAGCTGCGCGGTTTCGTGAAGATACGGGTCTTGTTGTTGAT 
               
               
                 CCTGAGAAAGAAGTTACTGTTACATCTGGTTGCACAGAAGCCATAGCTGCAGCTATG 
               
               
                 TTGGGTTTAATAAACCCTGGTGATGAAGTCATTCTCTTTGCACCGTTTTATGATTCCT 
               
               
                 ATGAAGCAACACTCTCTATGGCTGGTGCTAAAGTAAAAGGAATCACTTTACGTCCAC 
               
               
                 CGGACTTCTCCATCCCTTTGGAAGAGCTTAAAGCTGCGGTAACTAACAAGACTCGAG 
               
               
                 CCATCCTTATGAACACTCCGCACAACCCGACCGGGAAGATGTTCACTAGGGAGGAG 
               
               
                 CTTGAAACCATTGCATCTCTCTGCATTGAAAACGATGTGCTTGTGTTCTCGGATGAA 
               
               
                 GTATACGATAAGCTTGCGTTTGAAATGGATCACATTTCTATAGCTTCTCTTCCCGGTA 
               
               
                 TGTATGAAAGAACTGTGACCATGAATTCCCTGGGAAAGACTTTCTCTTTAACCGGAT 
               
               
                 GGAAGATCGGCTGGGCGATTGCGCCGCCTCATCTGACTTGGGGAGTTCGACAAGCA 
               
               
                 CACTCTTACCTCACATTCGCCACATCAACACCAGCACAATGGGCAGCCGTTGCAGCT 
               
               
                 CTCAAGGCACCAGAGTCTTACTTCAAAGAGCTGAAAAGAGATTACAATGTGAAAAA 
               
               
                 GGAGACTCTGGTTAAGGGTTTGAAGGAAGTCGGATTTACAGTGTTCCCATCGAGCGG 
               
               
                 GACTTACTTTGTGGTTGCTGATCACACTCCATTTGGAATGGAGAACGATGTTGCTTTC 
               
               
                 TGTGAGTATCTTATTGAAGAAGTTGGGGTCGTTGCGATCCCAACGAGCGTCTTTTAT 
               
               
                 CTGAATCCAGAAGAAGGGAAGAATTTGGTTAGGTTTGCGTTCTGTAAAGACGAAGA 
               
               
                 GACGTTGCGTGGTGCAATTGAGAGGATGAAGCAGAAGCTTAAGAGAAAAGTCTGA 
               
               
                   
               
               
                 SEQ ID NO: 21:  Arabidopsis  truncated GPT −45 construct amino acid sequence 
               
               
                 MATQNESTQKPVQVAKRLEKFKTTIFTQMSILAVKHGAINLGQGFPNFDGPDFVKEAAI 
               
               
                 QAIKDGKNQYARGYGIPQLNSAIAARFREDTGLVVDPEKEVTVTSGCTEAIAAAMLGLI 
               
               
                 NPGDEVILFAPFYDSYEATLSMAGAKVKGITLRPPDFSIPLEELKAAVTNKTRAILMNTP 
               
               
                 HNPTGKMFTREELETIASLCIENDVLVFSDEVYDKLAFEMDHISIASLPGMYERTVTMNS 
               
               
                 LGKTFSLTGWKIGWAIAPPHLTWGVRQAHSYLTFATSTPAQWAAVAALKAPESYFKEL 
               
               
                 KRDYNVKKETLVKGLKEVGFTVFPSSGTYFVVADHTPFGMENDVAFCEYLIEEVGVVAI 
               
               
                 PTSVFYLNPEEGKNLVRFAFCKDEETLRGAIERMKQKLKRKV 
               
               
                   
               
               
                 SEQ ID NO: 22: Tomato Rubisco promoter 
               
               
                 TOMATO RuBisCo rbcS3C promoter sequence from Kpn1 to Nco1 
               
               
                   GGTACC GTTTGAATCCTCCTTAAAGTTTTTCTCTGGAGAAACTGTAGTAATTTTACTT 
               
               
                 TGTTGTGTTCCCTTCATCTTTTGAATTAATGGCATTTGTTTTAATACTAATCTGCTTCT 
               
               
                 GAAACTTGTAATGTATGTATATCAGTTTCTTATAATTTATCCAAGTAATATCTTCCAT 
               
               
                 TCTCTATGCAATTGCCTGCATAAGCTCGACAAAAGAGTACATCAACCCCTCCTCCTC 
               
               
                 TGGACTACTCTAGCTAAACTTGAATTTCCCCTTAAGATTATGAAATTGATATATCCTT 
               
               
                 AACAAACGACTCCTTCTGTTGGAAAATGTAGTACTTGTCTTTCTTCTTTTGGGTATAT 
               
               
                 ATAGTTTATATACACCATACTATGTACAACATCCAAGTAGAGTGAAATGGATACATG 
               
               
                 TACAAGACTTATTTGATTGATTGATGACTTGAGTTGCCTTAGGAGTAACAAATTCTT 
               
               
                 AGGTCAATAAATCGTTGATTTGAAATTAATCTCTCTGTCTTAGACAGATAGGAATTA 
               
               
                 TGACTTCCAATGGTCCAGAAAGCAAAGTTCGCACTGAGGGTATACTTGGAATTGAG 
               
               
                 ACTTGCACAGGTCCAGAAACCAAAGTTCCCATCGAGCTCTAAAATCACATCTTTGGA 
               
               
                 ATGAAATTCAATTAGAGATAAGTTGCTTCATAGCATAGGTAAAATGGAAGATGTGA 
               
               
                 AGTAACCTGCAATAATCAGTGAAATGACATTAATACACTAAATACTTCATATGTAAT 
               
               
                 TATCCTTTCCAGGTTAACAATACTCTATAAAGTAAGAATTATCAGAAATGGGCTCAT 
               
               
                 CAAACTTTTGTACTATGTATTTCATATAAGGAAGTATAACTATACATAAGTGTATAC 
               
               
                 ACAACTTTATTCCTATTTTGTAAAGGTGGAGAGACTGTTTTCGATGGATCTAAAGCA 
               
               
                 ATATGTCTATAAAATGCATTGATATAATAATTATCTGAGAAAATCCAGAATTGGCGT 
               
               
                 TGGATTATTTCAGCCAAATAGAAGTTTGTACCATACTTGTTGATTCCTTCTAAGTTAA 
               
               
                 GGTGAAGTATCATTCATAAACAGTTTTCCCCAAAGTACTACTCACCAAGTTTCCCTTT 
               
               
                 GTAGAATTAACAGTTCAAATATATGGCGCAGAAATTACTCTATGCCCAAAACCAAA 
               
               
                 CGAGAAAGAAACAAAATACAGGGGTTGCAGACTTTATTTTCGTGTTAGGGTGTGTTT 
               
               
                 TTTCATGTAATTAATCAAAAAATATTATGACAAAAACATTTATACATATTTTTACTCA 
               
               
                 ACACTCTGGGTATCAGGGTGGGTTGTGTTCGACAATCAATATGGAAAGGAAGTATTT 
               
               
                 TCCTTATTTTTTTAGTTAATATTTTCAGTTATACCAAACATACCTTGTGATATTATTTT 
               
               
                 TAAAAATGAAAAACTCGTCAGAAAGAAAAAGCAAAAGCAACAAAAAAATTGCAAG 
               
               
                 TATTTTTTAAAAAAGAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGAG 
               
               
                 ATAAGGACGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGAA 
               
               
                 CCACAAAATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTCC 
               
               
                 GTTAGATAGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTAA 
               
               
                 CCAATTATTTCAGCA CC     ATG     G   
               
               
                   
               
               
                 SEQ ID NO: 23: Bamboo GPT DNA coding sequence 
               
               
                 ATGGCCTCCGCGGCCGTCTCCACCGTCGCCACCGCCGCCGACGGCGTCGCGAAGCC 
               
               
                 GACGGAGAAGCAGCCGGTACAGGTCGCAAAGCGTTTGGAAAAGTTTAAGACAACAA 
               
               
                 TTTTCACACAGATGAGCATGCTTGCCATCAAGCATGGAGCAATAAACCTCGGCCAGG 
               
               
                 GCTTTCCGAATTTTGATGGCCCTGACTTTGTGAAAGAAGCTGCTATTCAAGCTATCA 
               
               
                 ATGCTGGGAAGAATCAGTATGCAAGAGGATATGGTGTGCCTGAACTGAACTCGGCT 
               
               
                 GTTGCTGAAAGGTTCCTGAAGGACAGTGGCTTGCAAGTCGATCCCGAGAAGGAAGT 
               
               
                 TACTGTCACATCTGGGTGCACGGAAGCGATAGCTGCAACGATATTGGGTCTTATCAA 
               
               
                 CCCTGGCGATGAAGTGATCTTGTTTGCTCCATTCTATGATTCATACGAGGCTACGCTG 
               
               
                 TCGATGGCTGGTGCCAATGTAAAAGCCATTACTCTCCGTCCTCCAGATTTTGCAGTC 
               
               
                 CCTCTTGAGGAGCTAAAGGCCACAGTCTCTAAGAACACCAGAGCGATAATGATAAA 
               
               
                 CACACCACACAATCCTACTGGGAAAATGTTTTCTAGGGAAGAACTTGAATTCATTGC 
               
               
                 TACTCTCTGCAAGAAAAATGATGTGTTGCTTTTTGCTGATGAGGTCTATGACAAGTT 
               
               
                 GGCATTTGAGGCAGATCATATATCAATGGCTTCTATTCCTGGCATGTATGAGAGGAC 
               
               
                 TGTGACTATGAACTCTCTGGGGAAGACATTCTCTCTAACAGGATGGAAGATCGGTTG 
               
               
                 GGCAATAGCACCACCACACCTGACATGGGGTGTAAGGCAGGCACACTCATTCCTCA 
               
               
                 CATTTGCCACCTGCACACCAATGCAATCGGCGGCGGCGGCGGCTCTTAGAGCACCA 
               
               
                 GATAGCTACTATGGGGAGCTGAAGAGGGATTACGGTGCAAAGAAAGCGATACTAGT 
               
               
                 CGACGGACTCAAGGCTGCAGGTTTTATTGTTTACCCTTCAAGTGGAACATACTTTGT 
               
               
                 CATGGTCGATCACACCCCGTTTGGTTTCGACAATGATATTGAGTTCTGCGAGTATTTG 
               
               
                 ATCCGCGAAGTCGGTGTTGTCGCCATACCACCAAGCGTATTTTATCTCAACCCTGAG 
               
               
                 GATGGGAAGAACTTGGTGAGGTTCACCTTCTGCAAGGATGATGATACGCTGAGAGC 
               
               
                 CGCAGTTGAGAGGATGAAGACAAAGCTCAGGAAAAAATGA 
               
               
                   
               
               
                 SEQ ID NO: 24: Bamboo GPT amino acid sequence 
               
               
                 MASAAVSTVATAADGVAKPTEKQPVQVAKRLEKFKTTIFTQMSMLAIKHGAINLGQGF 
               
               
                 PNFDGPDFVKEAAIQAINAGKNQYARGYGVPELNSAVAERFLKDSGLQVDPEKEVTVTS 
               
               
                 GCTEAIAATILGLINPGDEVILFAPFYDSYEATLSMAGANVKAITLRPPDFAVPLEELKAT 
               
               
                 VSKNTRAIMINTPHNPTGKMFSREELEFIATLCKKNDVLLFADEVYDKLAFEADHISMAS 
               
               
                 IPGMYERTVTMNSLGKTFSLTGWKIGWAIAPPHLTWGVRQAHSFLTFATCTPMQSAAA 
               
               
                 AALRAPDSYYGELKRDYGAKKAILVDGLKAAGFIVYPSSGTYFVMVDHTPFGFDNDIEF 
               
               
                 CEYLIREVGVVAIPPSVFYLNPEDGKNLVRFTFCKDDDTLRAAVERMKTKLRKK 
               
               
                   
               
               
                 SEQ ID NO: 25: 1305.1 + rbcS3C promoter + cat1 intron with rice GPT gene. 
               
               
                 Cambia 1305.1 with (3′ end of) rbcS3C + rice GPT coding sequence. Underlined ATG is start 
               
               
                 site, parentheses are the cat1 intron and the underlined actagt is the spe1 cloning site used to splice 
               
               
                 in the rice gene. 
               
               
                 AAAAAAGAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGAGATAAGGA 
               
               
                 CGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGAACCACAAA 
               
               
                 ATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTCCGTTAGAT 
               
               
                 AGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTAACCAATTA 
               
               
                 TTTCAGCA          TAGATCTGAGG(GTAAATTTCTAGTTTTTCTCCTTCATTTTCTTG 
               
               
                 GTTAGGACCCTTTTCTCTTTTTATTTTTTTGAGCTTTGATCTTTCTTTAAACTGATCTA 
               
               
                 TTTTTTAATTGATTGGTTATGGTGTAAATATTACATAGCTTTAACTGATAATCTGATT 
               
               
                 ACTTTATTTCGTGTGTCTATGATGATGATGATAGTTACAG)AACCGACGAACTAGTAT 
               
               
                 GAATCTGGCCGGCTTTCTCGCCACGCCCGCGACCGCGACCGCGACGCGGCATGAGA 
               
               
                 TGCCGTTAAATCCCTCCTCCTCCGCCTCCTTCCTCCTCTCCTCGCTCCGCCGCTCGCTC 
               
               
                 GTCGCGTCGCTCCGGAAGGCCTCGCCGGCGGCGGCCGCGGCGCTCTCCCCCATGGCC 
               
               
                 TCCGCGTCCACCGTCGCCGCCGAGAACGGCGCCGCCAAGGCGGCGGCGGAGAAGCA 
               
               
                 GCAGCAGCAGCCTGTGCAGGTTGCAAAGCGGTTGGAAAAGTTTAAGACGACCATTT 
               
               
                 TCACACAGATGAGTATGCTTGCCATCAAGCATGGAGCAATAAACCTTGGCCAGGGTT 
               
               
                 TTCCGAATTTCGATGGCCCTGACTTTGTAAAAGAGGCTGCTATTCAAGCTATCAATG 
               
               
                 CTGGGAAGAATCAGTACGCAAGAGGATATGGTGTGCCTGAACTGAACTCAGCTATT 
               
               
                 GCTGAAAGATTCCTGAAGGACAGCGGACTGCAAGTCGATCCGGAGAAGGAAGTTAC 
               
               
                 TGTCACATCTGGATGCACAGAAGCTATAGCTGCAACAATTTTAGGTCTAATTAATCC 
               
               
                 AGGCGATGAAGTGATATTGTTTGCTCCATTCTATGATTCATATGAGGCTACCCTGTC 
               
               
                 AATGGCTGGTGCCAACGTAAAAGCCATTACTCTCCGTCCTCCAGATTTTTCAGTCCCT 
               
               
                 CTTGAAGAGCTAAAGGCTGCAGTCTCGAAGAACACCAGAGCTATTATGATAAACAC 
               
               
                 CCCGCACAATCCTACTGGGAAAATGTTTACAAGGGAAGAACTTGAGTTTATTGCCAC 
               
               
                 TCTCTGCAAGGAAAATGATGTGCTGCTTTTTGCTGATGAGGTCTACGACAAGTTAGC 
               
               
                 TTTTGAGGCAGATCATATATCAATGGCTTCTATTCCTGGCATGTATGAGAGGACCGT 
               
               
                 GACCATGAACTCTCTTGGGAAGACATTCTCTCTTACAGGATGGAAGATCGGTTGGGC 
               
               
                 AATCGCACCGCCACACCTGACATGGGGTGTAAGGCAGGCACACTCATTCCTCACGTT 
               
               
                 TGCGACCTGCACACCAATGCAAGCAGCTGCAGCTGCAGCTCTGAGAGCACCAGATA 
               
               
                 GCTACTATGAGGAACTGAGGAGGGATTATGGAGCTAAGAAGGCATTGCTAGTCAAC 
               
               
                 GGACTCAAGGATGCAGGTTTCATTGTCTATCCTTCAAGTGGAACATACTTCGTCATG 
               
               
                 GTCGACCACACCCCATTTGGTTTCGACAATGATATTGAGTTCTGCGAGTATTTGATTC 
               
               
                 GCGAAGTCGGTGTTGTCGCCATACCACCTAGTGTATTTTATCTCAACCCTGAGGATG 
               
               
                 GGAAGAACTTGGTGAGGTTCACCTTTTGCAAGGATGATGAGACGCTGAGAGCCGCG 
               
               
                 GTTGAGAGGATGAAGACAAAGCTCAGGAAAAAATGA 
               
               
                   
               
               
                 SEQ D NO: 26: HORDEUM GPT SEQUENCE IN VECTOR 
               
               
                 Cambia1305.1 with (3′ end of) rbcS3C + hordeum (IDI4) coding sequence. Underlined  ATG  is 
               
               
                 start site, parentheses are the cat1 intron and the underlined actagt is the spe1 cloning site used to 
               
               
                 splice in the hordeum gene. 
               
               
                 AAAAAAGAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGAGATAAGGA 
               
               
                 CGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGAACCACAAA 
               
               
                 ATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTCCGTTAGAT 
               
               
                 AGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTAACCAATTA 
               
               
                 TTTCAGCA          TAGATCTGAGG(GTAAATTTCTAGTTTTTCTCCTTCATTTTCTTG 
               
               
                 GTTAGGACCCTTTTCTCTTTTTATTTTTTTGAGCTTTGATCTTTCTTTAAACTGATCTA 
               
               
                 TTTTTTAATTGATTGGTTATGGTGTAAATATTACATAGCTTTAACTGATAATCTGATT 
               
               
                 ACTTTATTTCGTGTGTCTATGATGATGATGATAGTTACAG)AACCGACGA          AT 
               
               
                 GGCATCCGCCCCCGCCTCCGCCTCCGCGGCCCTCTCCACCGCCGCCCCCGCCGACAA 
               
               
                 CGGGGCCGCCAAGCCCACGGAGCAGCGGCCGGTACAGGTGGCTAAGCGATTGGAG 
               
               
                 AAGTTCAAAACAACAATTTTCACACAGATGAGCATGCTCGCAGTGAAGCATGGAGC 
               
               
                 AATAAACCTTGGACAGGGGTTTCCCAATTTTGATGGCCCTGACTTTGTCAAAGATGC 
               
               
                 TGCTATTGAGGCTATCAAAGCTGGAAAGAATCAGTATGCAAGAGGATATGGTGTGC 
               
               
                 CTGAATTGAACTCAGCTGTTGCTGAGAGATTTCTCAAGGACAGTGGATTGCACATCG 
               
               
                 ATCCTGATAAGGAAGTTACTGTTACATCTGGGTGCACAGAAGCAATAGCTGCAACG 
               
               
                 ATATTGGGTCTGATCAACCCTGGGGATGAAGTCATACTGTTTGCTCCATTCTATGATT 
               
               
                 CTTATGAGGCTACACTGTCCATGGCTGGTGCGAATGTCAAAGCCATTACACTCCGCC 
               
               
                 CTCCGGACTTTGCAGTCCCTCTTGAAGAGCTAAAGGCTGCAGTCTCGAAGAATACCA 
               
               
                 GAGCAATAATGATTAATACACCTCACAACCCTACCGGGAAAATGTTCACAAGGGAG 
               
               
                 GAACTTGAGTTCATTGCTGATCTCTGCAAGGAAAATGACGTGTTGCTCTTTGCCGAT 
               
               
                 GAGGTCTACGACAAGCTGGCGTTTGAGGCGGATCACATATCAATGGCTTCTATTCCT 
               
               
                 GGCATGTATGAGAGGACCGTCACTATGAACTCCCTGGGGAAGACGTTCTCCTTGACC 
               
               
                 GGATGGAAGATCGGCTGGGCGATAGCACCACCGCACCTGACATGGGGCGTAAGGCA 
               
               
                 GGCACACTCCTTCCTCACATTCGCCACCTCCACGCCGATGCAATCAGCAGCGGCGGC 
               
               
                 GGCCCTGAGAGCACCGGACAGCTACTTTGAGGAGCTGAAGAGGGACTACGGCGCAA 
               
               
                 AGAAAGCGCTGCTGGTGGACGGGCTCAAGGCGGCGGGCTTCATCGTCTACCCTTCG 
               
               
                 AGCGGAACCTACTTCATCATGGTCGACCACACCCCGTTCGGGTTCGACAACGACGTC 
               
               
                 GAGTTCTGCGAGTACTTGATCCGCGAGGTCGGCGTCGTGGCCATCCCGCCAAGCGTG 
               
               
                 TTCTACCTGAACCCGGAGGACGGGAAGAACCTGGTGAGGTTCACCTTCTGCAAGGA 
               
               
                 CGACGACACGCTAAGGGCGGCGGTGGACAGGATGAAGGCCAAGCTCAGGAAGAAA 
               
               
                 TGATTGAGGGGCG           
               
               
                   
               
               
                 SEQ ID NO: 27 Expression cassette,  Arabidopsis  GPT coding sequence (ATG underlined) under 
               
               
                 control of CMV 35S promoter (italics; promoter from Cambia 1201) 
               
               
                 
                   CATGGAGTCAAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACA 
                 
               
               
                 
                   GTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAG 
                 
               
               
                 
                   CACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAA 
                 
               
               
                 
                   TTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTAT 
                 
               
               
                 
                   CTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGC 
                 
               
               
                 
                   GATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCC 
                 
               
               
                 
                   CCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTG 
                 
               
               
                 
                   GATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAG 
                 
               
               
                 
                   ACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGAACACGGGGGACTCTTGACC 
                   A 
                 
               
               
                   TG TACCTGGACATAAATGGTGTGATGATCAAACAGTTTAGCTTCAAAGCCTCTCTTC 
               
               
                 TCCCATTCTCTTCTAATTTCCGACAAAGCTCCGCCAAAATCCATCGTCCTATCGGAGC 
               
               
                 CACCATGACCACAGTTTCGACTCAGAACGAGTCTACTCAAAAACCCGTCCAGGTGG 
               
               
                 CGAAGAGATTAGAGAAGTTCAAGACTACTATTTTCACTCAAATGAGCATATTGGCAG 
               
               
                 TTAAACATGGAGCGATCAATTTAGGCCAAGGCTTTCCCAATTTCGACGGTCCTGATT 
               
               
                 TTGTTAAAGAAGCTGCGATCCAAGCTATTAAAGATGGTAAAAACCAGTATGCTCGTG 
               
               
                 GATACGGCATTCCTCAGCTCAACTCTGCTATAGCTGCGCGGTTTCGTGAAGATACGG 
               
               
                 GTCTTGTTGTTGATCCTGAGAAAGAAGTTACTGTTACATCTGGTTGCACAGAAGCCA 
               
               
                 TAGCTGCAGCTATGTTGGGTTTAATAAACCCTGGTGATGAAGTCATTCTCTTTGCACC 
               
               
                 GTTTTATGATTCCTATGAAGCAACACTCTCTATGGCTGGTGCTAAAGTAAAAGGAAT 
               
               
                 CACTTTACGTCCACCGGACTTCTCCATCCCTTTGGAAGAGCTTAAAGCTGCGGTAAC 
               
               
                 TAACAAGACTCGAGCCATCCTTATGAACACTCCGCACAACCCGACCGGGAAGATGT 
               
               
                 TCACTAGGGAGGAGCTTGAAACCATTGCATCTCTCTGCATTGAAAACGATGTGCTTG 
               
               
                 TGTTCTCGGATGAAGTATACGATAAGCTTGCGTTTGAAATGGATCACATTTCTATAG 
               
               
                 CTTCTCTTCCCGGTATGTATGAAAGAACTGTGACCATGAATTCCCTGGGAAAGACTT 
               
               
                 TCTCTTTAACCGGATGGAAGATCGGCTGGGCGATTGCGCCGCCTCATCTGACTTGGG 
               
               
                 GAGTTCGACAAGCACACTCTTACCTCACATTCGCCACATCAACACCAGCACAATGGG 
               
               
                 CAGCCGTTGCAGCTCTCAAGGCACCAGAGTCTTACTTCAAAGAGCTGAAAAGAGAT 
               
               
                 TACAATGTGAAAAAGGAGACTCTGGTTAAGGGTTTGAAGGAAGTCGGATTTACAGT 
               
               
                 GTTCCCATCGAGCGGGACTTACTTTGTGGTTGCTGATCACACTCCATTTGGAATGGA 
               
               
                 GAACGATGTTGCTTTCTGTGAGTATCTTATTGAAGAAGTTGGGGTCGTTGCGATCCC 
               
               
                 AACGAGCGTCTTTTATCTGAATCCAGAAGAAGGGAAGAATTTGGTTAGGTTTGCGTT 
               
               
                 CTGTAAAGACGAAGAGACGTTGCGTGGTGCAATTGAGAGGATGAAGCAGAAGCTTA 
               
               
                 AGAGAAAAGTCTGA 
               
               
                   
               
               
                 SEQ ID NO: 28 Cambia p1305.1 with (3′ end of) rbcS3C +  Arabidopsis  GPT coding sequence. 
               
               
                 Underlined ATG is start site, parentheses are the cat1 intron and the underlined actagt is the spe1 
               
               
                 cloning site used to splice in the  Arabidopsis  gene. 
               
               
                 AAAAAAGAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGAGATAAGGA 
               
               
                 CGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGAACCACAAA 
               
               
                 ATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTCCGTTAGAT 
               
               
                 AGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTAACCAATTA 
               
               
                 TTTCAGCA          TAGATCTGAGG(GTAAATTTCTAGTTTTTCTCCTTCATTTTCTTG 
               
               
                 GTTAGGACCCTTTTCTCTTTTTATTTTTTTGAGCTTTGATCTTTCTTTAAACTGATCTA 
               
               
                 TTTTTTAATTGATTGGTTATGGTGTAAATATTACATAGCTTTAACTGATAATCTGATT 
               
               
                 ACTTTATTTCGTGTGTCTATGATGATGATGATAGTTACAG)AACCGACGA          AT 
               
               
                 GTACCTGGACATAAATGGTGTGATGATCAAACAGTTTAGCTTCAAAGCCTCTCTTCT 
               
               
                 CCCATTCTCTTCTAATTTCCGACAAAGCTCCGCCAAAATCCATCGTCCTATCGGAGC 
               
               
                 CACCATGACCACAGTTTCGACTCAGAACGAGTCTACTCAAAAACCCGTCCAGGTGG 
               
               
                 CGAAGAGATTAGAGAAGTTCAAGACTACTATTTTCACTCAAATGAGCATATTGGCAG 
               
               
                 TTAAACATGGAGCGATCAATTTAGGCCAAGGCTTTCCCAATTTCGACGGTCCTGATT 
               
               
                 TTGTTAAAGAAGCTGCGATCCAAGCTATTAAAGATGGTAAAAACCAGTATGCTCGTG 
               
               
                 GATACGGCATTCCTCAGCTCAACTCTGCTATAGCTGCGCGGTTTCGTGAAGATACGG 
               
               
                 GTCTTGTTGTTGATCCTGAGAAAGAAGTTACTGTTACATCTGGTTGCACAGAAGCCA 
               
               
                 TAGCTGCAGCTATGTTGGGTTTAATAAACCCTGGTGATGAAGTCATTCTCTTTGCACC 
               
               
                 GTTTTATGATTCCTATGAAGCAACACTCTCTATGGCTGGTGCTAAAGTAAAAGGAAT 
               
               
                 CACTTTACGTCCACCGGACTTCTCCATCCCTTTGGAAGAGCTTAAAGCTGCGGTAAC 
               
               
                 TAACAAGACTCGAGCCATCCTTATGAACACTCCGCACAACCCGACCGGGAAGATGT 
               
               
                 TCACTAGGGAGGAGCTTGAAACCATTGCATCTCTCTGCATTGAAAACGATGTGCTTG 
               
               
                 TGTTCTCGGATGAAGTATACGATAAGCTTGCGTTTGAAATGGATCACATTTCTATAG 
               
               
                 CTTCTCTTGCCGGTATGTATGAAAGAACTGTGACCATGAATTCCCTGGGAAAGACTT 
               
               
                 TCTCTTTAACCGGATGGAAGATCGGCTGGGCGATTGCGCCGCCTCATCTGACTTGGG 
               
               
                 GAGTTCGACAAGCACACTCTTACCTCACATTCGCCACATCAACACCAGCACAATGGG 
               
               
                 CAGCCGTTGCAGCTCTCAAGGCACCAGAGTCTTACTTCAAAGAGCTGAAAAGAGAT 
               
               
                 TACAATGTGAAAAAGGAGACTCTGGTTAAGGGTTTGAAGGAAGTCGGATTTACAGT 
               
               
                 GTTCCCATCGAGCGGGACTTACTTTGTGGTTGCTGATCACACTCCATTTGGAATGGA 
               
               
                 GAACGATGTTGCTTTCTGTGAGTATCTTATTGAAGAAGTTGGGGTCGTTGCGATCCC 
               
               
                 AACGAGCGTCTTTTATCTGAATCCAGAAGAAGGGAAGAATTTGGTTAGGTTTGCGTT 
               
               
                 CTGTAAAGACGAAGAGACGTTGCGTGGTGCAATTGAGAGGATGAAGCAGAAGCTTA 
               
               
                 AGAGAAAAGTCTGA 
               
               
                   
               
               
                 SEQ ID NO: 29  Arabidopsis  GPT coding sequence (mature protein, no targeting sequence) 
               
               
                 GTGGCGAAGAGATTAGAGAAGTTCAAGACTACTATTTTCACTCAAATGAGCATATTG 
               
               
                 GCAGTTAAACATGGAGCGATCAATTTAGGCCAAGGCTTTCCCAATTTCGACGGTCCT 
               
               
                 GATTTTGTTAAAGAAGCTGCGATCCAAGCTATTAAAGATGGTAAAAACCAGTATGCT 
               
               
                 CGTGGATACGGCATTCCTCAGCTCAACTCTGCTATAGCTGCGCGGTTTCGTGAAGAT 
               
               
                 ACGGGTCTTGTTGTTGATCCTGAGAAAGAAGTTACTGTTACATCTGGTTGCACAGAA 
               
               
                 GCCATAGCTGCAGCTATGTTGGGTTTAATAAACCCTGGTGATGAAGTCATTCTCTTT 
               
               
                 GCACCGTTTTATGATTCCTATGAAGCAACACTCTCTATGGCTGGTGCTAAAGTAAAA 
               
               
                 GGAATCACTTTACGTCCACCGGACTTCTCCATCCCTTTGGAAGAGCTTAAAGCTGCG 
               
               
                 GTAACTAACAAGACTCGAGCCATCCTTATGAACACTCCGCACAACCCGACCGGGAA 
               
               
                 GATGTTCACTAGGGAGGAGCTTGAAACCATTGCATCTCTCTGCATTGAAAACGATGT 
               
               
                 GCTTGTGTTCTCGGATGAAGTATACGATAAGCTTGCGTTTGAAATGGATCACATTTC 
               
               
                 TATAGCTTCTCTTCCCGGTATGTATGAAAGAACTGTGACCATGAATTCCCTGGGAAA 
               
               
                 GACTTTCTCTTTAACCGGATGGAAGATCGGCTGGGCGATTGCGCCGCCTCATCTGAC 
               
               
                 TTGGGGAGTTCGACAAGCACACTCTTACCTCACATTCGCCACATCAACACCAGCACA 
               
               
                 ATGGGCAGCCGTTGCAGCTCTCAAGGCACCAGAGTCTTACTTCAAAGAGCTGAAAA 
               
               
                 GAGATTACAATGTGAAAAAGGAGACTCTGGTTAAGGGTTTGAAGGAAGTCGGATTT 
               
               
                 ACAGTGTTCCCATCGAGCGGGACTTACTTTGTGGTTGCTGATCACACTCCATTTGGA 
               
               
                 ATGGAGAACGATGTTGCTTTCTGTGAGTATCTTATTGAAGAAGTTGGGGTCGTTGCG 
               
               
                 ATCCCAACGAGCGTCTTTTATCTGAATCCAGAAGAAGGGAAGAATTTGGTTAGGTTT 
               
               
                 GCGTTCTGTAAAGACGAAGAGACGTTGCGTGGTGCAATTGAGAGGATGAAGCAGAA 
               
               
                 GCTTAAGAGAAAAGTCTGA 
               
               
                   
               
               
                 SEQ ID NO: 30  Arabidopsis  GPT amino acid sequence (mature protein, no targeting sequence) 
               
               
                 VAKRLEKFKTTIFTQMSILAVKHGAINLGQGFPNFDGPDFVKEAAIQAIKDGKNQYARG 
               
               
                 YGIPQLNSAIAARFREDTGLVVDPEKEVTVTSGCTEAIAAAMLGLINPGDEVILFAPFYDS 
               
               
                 YEATLSMAGAKVKGITLRPPDFSIPLEELKAAVTNKTRAILMNTPHNPTGKMFTREELET 
               
               
                 IASLCIENDVLVFSDEVYDKLAFEMDHISIASLPGMYERTVTMNSLGKTFSLTGWKIGWA 
               
               
                 IAPPHLTWGVRQAHSYLTFATSTPAQWAAVAALKAPESYFKELKRDYNVKKETLVKGL 
               
               
                 KEVGFTVFPSSGTYFVVADHTPFGMENDVAFCEYLIEEVGVVAIPTSVFYLNPEEGKNLV 
               
               
                 RFAFCKDEETLRGAIERMKQKLKRKV 
               
               
                   
               
               
                 SEQ ID NO: 31 Grape GPT amino acid sequence (mature protein, no targeting sequence) 
               
               
                 VAKRLEKFKTTIFTQMSMLAIKHGAINLGQGFPNFDGPEFVKEAAIQAIKDGKNQYARG 
               
               
                 YGVPDLNSAVADRFKKDTGLVVDPEKEVTVTSGCTEAIAATMLGLINPGDEVILFAPFY 
               
               
                 DSYEATLSMAGAQIKSITLRPPDFAVPMDELKSAISKNTRAILINTPHNPTGKMFTREELN 
               
               
                 VIASLCIENDVLVFTDEVYDKLAFEMDHISMASLPGMYERTVTMNSLGKTFSLTGWKIG 
               
               
                 WTVAPPHLTWGVRQAHSFLTFATCTPMQWAAATALRAPDSYYEELKRDYSAKKAILV 
               
               
                 EGLKAVGFRVYPSSGTYFVVVDHTPFGLKDDIAFCEYLIKEVGVVAIPTSVFYLHPEDGK 
               
               
                 NLVRFTFCKDEGTLRAAVERMKEKLKPKQ 
               
               
                   
               
               
                 SEQ ID NO: 32 Rice GPT amino acid sequence (mature protein, no targeting sequence) 
               
               
                 VAKRLEKFKTTIFTQMSMLAIKHGAINLGQGFPNFDGPDFVKEAAIQAINAGKNQYARG 
               
               
                 YGVPELNSAIAERFLKDSGLQVDPEKEVTVTSGCTEAIAATILGLINPGDEVILFAPFYDS 
               
               
                 YEATLSMAGANVKAITLRPPDFSVPLEELKAAVSKNTRAIMINTPHNPTGKMFTREELEF 
               
               
                 IATLCKENDVLLFADEVYDKLAFEADHISMASIPGMYERTVTMNSLGKTFSLTGWKIGW 
               
               
                 AIAPPHLTWGVRQAHSFLTFATCTPMQAAAAAALRAPDSYYEELRRDYGAKKALLVNG 
               
               
                 LKDAGFIVYPSSGTYFVMVDHTPFGFDNDIEFCEYLIREVGVVAIPPSVFYLNPEDGKNL 
               
               
                 VRFTFCKDDETLRAAVERMKTKLRKK 
               
               
                   
               
               
                 SEQ ID NO: 33 Soybean GPT amino acid sequence (−1 mature protein, no targeting sequence) 
               
               
                 AKRLEKFQTTIFTQMSLLAIKHGAINLGQGFPNFDGPEFVKEAAIQAIRDGKNQYARGYG 
               
               
                 VPDLNIAIAERFKKDTGLVVDPEKEITVTSGCTEAIAATMIGLINPGDEVIMFAPFYDSYE 
               
               
                 ATLSMAGAKVKGITLRPPDFAVPLEELKSTISKNTRAILINTPHNPTGKMFTREELNCIAS 
               
               
                 LCIENDVLVFTDEVYDKLAFDMEHISMASLPGMFERTVTLNSLGKTFSLTGWKIGWAIA 
               
               
                 PPHLSWGVRQAHAFLTFATAHPFQCAAAAALRAPDSYYVELKRDYMAKRAILIEGLKA 
               
               
                 VGFKVFPSSGTYFVVVDHTPFGLENDVAFCEYLVKEVGVVAIPTSVFYLNPEEGKNLVR 
               
               
                 FTFCKDEETIRSAVERMKAKLRKVD 
               
               
                   
               
               
                 SEQ ID NO: 34 Barley GPT amino acid sequence (mature protein, no targeting sequence) 
               
               
                 VAKRLEKFKTTIFTQMSMLAVKHGAINLGQGFPNFDGPDFVKDAAIEAIKAGKNQYAR 
               
               
                 GYGVPELNSAVAERFLKDSGLHIDPDKEVTVTSGCTEAIAATILGLINPGDEVILFAPFYD 
               
               
                 SYEATLSMAGANVKAITLRPPDFAVPLEELKAAVSKNTRAIMINTPHNPTGKMFTREELE 
               
               
                 FIADLCKENDVLLFADEVYDKLAFEADHISMASIPGMYERTVTMNSLGKTFSLTGWKIG 
               
               
                 WAIAPPHLTWGVRQAHSFLTFATSTPMQSAAAAALRAPDSYFEELKRDYGAKKALLVD 
               
               
                 GLKAAGFIVYPSSGTYFIMVDHTPFGFDNDVEFCEYLIREVGVVAIPPSVFYLNPEDGKN 
               
               
                 LVRFTFCKDDDTLRAAVDRMKAKLRKK 
               
               
                   
               
               
                 SEQ ID NO: 35 Zebra fish GPT amino acid sequence (mature protein, no targeting sequence) 
               
               
                 VAKRLEKFKTTIFTQMSMLAIKHGAINLGQGFPNFDGPDFVKEAAIQAIRDGNNQYARG 
               
               
                 YGVPDLNIAISERYKKDTGLAVDPEKEITVTSGCTEAIAATVLGLINPGDEVIVFAPFYDS 
               
               
                 YEATLSMAGAKVKGITLRPPDFALPIEELKSTISKNTRAILLNTPHNPTGKMFTPEELNTIA 
               
               
                 SLCIENDVLVFSDEVYDKLAFDMEHISIASLPGMFERTVTMNSLGKTFSLTGWKIGWAIA 
               
               
                 PPHLTWGVRQAHAFLTFATSNPMQWAAAVALRAPDSYYTELKRDYMAKRSILVEGLK 
               
               
                 AVGFKVFPSSGTYFVVVDHTPFGHENDIAFCEYLVKEVGVVAIPTSVFYLNPEEGKNLV 
               
               
                 RFTFCKDEGTLRAAVDRMKEKLRK 
               
               
                   
               
               
                 SEQ ID NO: 36 Bamboo GPT amino acid sequence (mature protein, no targeting sequence) 
               
               
                 VAKRLEKFKTTIFTQMSMLAIKHGAINLGQGFPNFDGPDFVKEAAIQAINAGKNQYARG 
               
               
                 YGVPELNSAVAERFLKDSGLQVDPEKEVTVTSGCTEAIAATILGLINPGDEVILFAPFYDS 
               
               
                 YEATLSMAGANVKAITLRPPDFAVPLEELKATVSKNTRAIMINTPHNPTGKMFSREELEF 
               
               
                 IATLCKKNDVLLFADEVYDKLAFEADHISMASIPGMYERTVTMNSLGKTFSLTGWKIGW 
               
               
                 AIAPPHLTWGVRQAHSFLTFATCTPMQSAAAAALRAPDSYYGELKRDYGAKKAILVDG 
               
               
                 LKAAGFIVYPSSGTYFVMVDHTPFGFDNDIEFCEYLIREVGVVAIPPSVFYLNPEDGKNL 
               
               
                 VRFTFCKDDDTLRAAVERMKTKLRKK 
               
               
                   
               
               
                 SEQ ID NO: 37 Rice rubisco promoter deposited in NCBI GenBank: AF143510.1 
               
               
                 Pst1 cloning sites in bold; Nco1 cloning site in italics, cat1 intron and part of Gus plus protein 
               
               
                 from Cambia 1305.1 vector in bold underline (sequence removed and not translated), 3′ terminal 
               
               
                 Spe1 cloning site in double underline. The construct also includes a Pml1 1305.1 cloning site 
               
               
                 CACGTG (also cuts in rice rbsc promoter), and a Zra1 cloning site GACGTC, which can be 
               
               
                 added by PCR to clone into PmlI site of vector). 
               
               
                   CTGCAG CAAAGAAACGTTATTAGTTGGTGCTTTTGGTGGTAGGAATGTAGTTTTCTG 
               
               
                 ACAAAGTCAATTACTGAATATAAAAAAAATCTGCACAGCTCTGCGTCAACAGTTGTC 
               
               
                 CAAGGGATGCCTCAAAAATCTGTGCAGATTATCAGTCGTCACGCAGAAGCAGAACA 
               
               
                 TCATGGTGTGCTAGGTCAGCTTCTTGCATTGGGCCATGAATCCGGTTGGTTGTTAATC 
               
               
                 TCTCCTCTCTTATTCTCTTATATTAAGATGCATAACTCTTTTATGTAGTCTAAAAAAA 
               
               
                 AATCCAGTGGATCGGATAGTAGTACGTCATGGTGCCATTAGGTACCGTTGAACCTAA 
               
               
                 CAGATATTTATGCATGTGTATATATATAGCTATATAGACAAAATTGATGCCGATTAT 
               
               
                 AGACCCAAAAGCAATAGGTATATATAATATAATACAGACCACACCACCAAACTAAG 
               
               
                 AATCGATCAAATAGACAAGGCATGTCTCCAAATTGTCTTAAACTATTTCCGTAGGTT 
               
               
                 CAGCCGTTCAGGAGTCGAATCAGCCTCTGCCGGCGTTTTCTTTGCACGTACGACGGA 
               
               
                 CACACATGGGCATACCATATAGCTGGTCCATGACATTAGGAGAGAGAACGTACGTG 
               
               
                 TTGACCTGTAGCTGAGATATAACAAGGTTGATTATAATATCACCAAACATGAAATCA 
               
               
                 TCCAAGGATGACCCATAACTATCACTACTATAGTACTGCATCTGGTAAAAGAAATTG 
               
               
                 TATAGACTCTATTTCGAGCACTACCACATAACGCCTGCAATGTGACACCCTACCTAT 
               
               
                 TCACTAATGTGCCTCTTCCCACACGCTTTCCACCCGTACTGCTCACAGCTTTAAGAAC 
               
               
                 CAGAACAAATGAGTAATATTAGTGTCGGTTCATGGCTAAAACCAGCACTGATGTAC 
               
               
                 ATGACCACATATGTCAAATGCTGCTTCTAGGCATGACCCGCTCTTACTAATACCTAC 
               
               
                 TCATCGCTAGAAGAATTTTCGGCTGATAAATTTTCAATTTAAGCAAGAGTTATCTGC 
               
               
                 GTTGGTTCATAACTCAAACTGATGGCCCCAACCATATTAGTGCAAATTTCACATATG 
               
               
                 ATCATAACCTTTTCATATGAAATCGGATCGAGATGAACTTTATATAAACATTGTAGC 
               
               
                 TGTCGATGATACCTACAATTTTATAGTTCACAACCTTTTTATTTCAAGTCATTTAAAT 
               
               
                 GCCCAAATAGGTGTTTCAAATCTCAGATAGAAATGTTCAAAAGTAAAAAAGGTCCC 
               
               
                 TATCATAACATAATTGATATGTAAGTGAGTTGGAAAAAGATAAGTACGTGTGAGAG 
               
               
                 AGATCGGGGATCAAATTCTGGTGTAATAATGTATGTATTTCAGTCATAAAAATTGGT 
               
               
                 AGCAGTAGTTGGGGCTCTGTATATATACCGGTAAGGATGGGATGGTAGTAGAATAA 
               
               
                 TTCTTTTTTTGTTTTTAGTTTTTTCTGGTCCAAAATTTCAAATTTGGATCCCTTACTTG 
               
               
                 TACCAACTAATATTAATGAGTGTTGAGGGTAGTAGAGGTGCAACTTTACCATAATCC 
               
               
                 CTCTGTTTCAGGTTATAAGACGTTTTGACTTTAAATTTGACCAAGTTTATGCGCAAAT 
               
               
                 ATAGTAATATTTATAATACTATATTAGTTTCATTAAATAAATAATTGAATATATTTTC 
               
               
                 ATAATAAATTTGTGTTGAGTTCAAAATATTATTAATTTTTTCTACAAACTTGGTCAAA 
               
               
                 CTTGAAGCAGTTTGACTTTGACCAAAGTCAAAACGTCTTATAACTTGAAACGGATGG 
               
               
                 ATTACTTTTTTTGTGGGGACAAGTTTACAATGTTTAATAAAGCACAATCCATCTTAAT 
               
               
                 GTTTTCAAGCTGAATATTGTAAAATTCATGGATAAACCAGCTTCTAAATGTTTAACC 
               
               
                 GGGAAAATGTCGAACGACAAATTAATATTTTTAAGTGATGGGGAGTATTAATTAAG 
               
               
                 GAGTGACAACTCAACTTTCAATATCGTACTAAACTGTGGGATTTATTTTCTAAAATTT 
               
               
                 TATACCCTGCCAATTCACGTGTTGTAGATCTTTTTTTTTCACTAACCGACACCAGGTA 
               
               
                 TATCAATTTTATTGAATATAGCAGCAAAAAGAATGTGTTGTACTTGTAAACAAAAAG 
               
               
                 CAAACTGTACATAAAAAAAAATGCACTCCTATATAATTAAGCTCATAAAGATGCTTT 
               
               
                 GCTTCGTGAGGGCCCAAGTTTTGATGACCTTTTGCTTGATCTCGAAATTAAAATTTAA 
               
               
                 GTACTGTTAAGGGAGTTCACACCACCATCAATTTTCAGCCTGAAGAAACAGTTAAAC 
               
               
                 AACGACCCCGATGACCAGTCTACTGCTCTCCACATACTAGCTGCATTATTGATCACA 
               
               
                 AAACAAAACAAAACGAAATAAAAATCAGCAGCGAGAGTGTGCAGAGAGAGACAAA 
               
               
                 GGTGATCTGGCGTGGATATCTCCCCATCCATCCTCACCCGCGCTGCCCATCACTCGC 
               
               
                 CGCCGCATACTCCATCATGTGGAGAGAGGAAGACGAGGACCACAGCCAGAGCCCGG 
               
               
                 GTCGAGATGCCACCACGGCCACAACCCACGAGCCCGGCGCGACACCACCGCGCGCG 
               
               
                 CGTGAGCCAGCCACAAACGCCCGCGGATAGGCGCGCGCACGCCGGCCAATCCTACC 
               
               
                 ACATCCCCGGCCTCCGCGGCTCGCGAGCGCCGCTGCCATCCGATCCGCTGAGTTTTG 
               
               
                 GCTATTTATACGTACCGCGGGAGCCTGTGTGCAGAGCAGTGCATCTCAAGAAGTACT 
               
               
                 CGAGCAAAGAAGGAGAGAGCTTGGTGAG CTGCAG   CC     ATG     G TAGATCTGAGG   GTAA     
               
               
                 
                   
                     ATTTCTAGTTTTTCTCCTTCATTTTCTTGGTTAGGACCCTTTTCTCTTTTTATTTT 
                   
                 
               
               
                 
                   
                     TTTGAGCTTTGATCTTTCTTTAAACTGATCTATTTTTTAATTGATTGGTTATGGT 
                   
                 
               
               
                 
                   
                     GTAAATATTACATAGCTTTAACTGATAATCTGATTACTTTATTTCGTGTGTCTAT 
                   
                 
               
               
                     GATGATGATGATAGTTACAG   AACCGACGA ACTAGT   
               
               
                   
               
               
                 SEQ ID NO: 38 Hordeum GS1 coding sequence 
               
               
                 GCGCAGGCGGTTGTGCAGGCGATGCAGTGCCAGGTGGGGGTGAGGGGCAGGACGG 
               
               
                 CCGTCCCGGCGAGGCAGCCCGCGGGCAGGGTGTGGGGCGTCAGGAGGGCCGCCCGC 
               
               
                 GCCACCTCCGGGTTCAAGGTGCTGGCGCTCGGCCCGGAGACCACCGGGGTCATCCA 
               
               
                 GAGGATGCAGCAGCTGCTCGACATGGACACCACGCCCTTCACCGACAAGATCATCG 
               
               
                 CCGAGTACATCTGGGTTGGAGGATCTGGAATTGACCTCAGAAGCAAATCAAGGACG 
               
               
                 ATTTCGAAGCCAGTGGAGGACCCGTCAGAGCTGCCGAAATGGAACTACGACGGATC 
               
               
                 GAGCACGGGGCAGGCTCCTGGGGAAGACAGTGAAGTCATCCTATACCCACAGGCCA 
               
               
                 TATTCAAGGACCCATTCCGAGGAGGCAACAACATACTGGTTATCTGTGACACCTACA 
               
               
                 CACCACAGGGGGAACCCATCCCTACTAACAAACGCCACATGGCTGCACAAATCTTC 
               
               
                 AGTGACCCCAAGGTCACTTCACAAGTGCCATGGTTCGGAATCGAACAGGAGTACAC 
               
               
                 TCTGATGCAGAGGGATGTGAACTGGCCTCTTGGCTGGCCTGTTGGAGGGTACCCTGG 
               
               
                 CCCCCAGGGTCCATACTACTGCGCCGTAGGATCAGACAAGTCATTTGGCCGTGACAT 
               
               
                 ATCAGATGCTCACTACAAGGCGTGCCTTTACGCTGGAATTGAAATCAGTGGAACAA 
               
               
                 ACGGGGAGGTCATGCCTGGTCAGTGGGAGTACCAGGTTGGACCCAGCGTTGGTATT 
               
               
                 GATGCAGGAGACCACATATGGGCTTCCAGATACATTCTCGAGAGAATCACGGAGCA 
               
               
                 AGCTGGTGTGGTGCTCACCCTTGACCCAAAACCAATCCAGGGTGACTGGAACGGAG 
               
               
                 CTGGCTGCCACACAAACTACAGCACATTGAGCATGCGCGAGGATGGAGGTTTCGAC 
               
               
                 GTGATCAAGAAGGCAATCCTGAACCTTTCACTTCGCCATGACTTGCACATAGCCGCA 
               
               
                 TATGGTGAAGGAAACGAGCGGAGGTTGACAGGGCTACACGAGACAGCTAGCATATC 
               
               
                 AGACTTCTCATGGGGTGTGGCGAACCGTGGCTGCTCTATTCGTGTGGGGCGAGACAC 
               
               
                 CGAGGCGAAGGGCAAAGGATACCTGGAGGACCGTCGCCCGGCCTCCAACATGGACC 
               
               
                 CGTACACCGTGACGGCGCTGCTGGCCGAGACCACGATCCTGTGGGAGCCGACCCTC 
               
               
                 GAGGCGGAGGCCCTCGCTGCCAAGAAGCTGGCGCTGAAGGTATGA 
               
               
                   
               
               
                 SEQ ID NO: 39 Hordeum GS1 amino acid sequence 
               
               
                 AQAVVQAMQCQVGVRGRTAVPARQPAGRVWGVRRAARATSGFKVLALGPETTGVIQ 
               
               
                 RMQQLLDMDTTPFTDKIIAEYIWVGGSGIDLRSKSRTISKPVEDPSELPKWNYDGSSTGQ 
               
               
                 APGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPQGEPIPTNKRHMAAQIFSDPKVTSQV 
               
               
                 PWFGIEQEYTLMQRDVNWPLGWPVGGYPGPQGPYYCAVGSDKSFGRDISDAHYKACL 
               
               
                 YAGIEISGTNGEVMPGQWEYQVGPSVGIDAGDHIWASRYILERITEQAGVVLTLDPKPIQ 
               
               
                 GDWNGAGCHTNYSTLSMREDGGFDVIKKAILNLSLRHDLHIAAYGEGNERRLTGLHET 
               
               
                 ASISDFSWGVANRGCSIRVGRDTEAKGKGYLEDRRPASNMDPYTVTALLAETTILWEPT 
               
               
                 LEAEALAAKKLALKV 
               
               
                   
               
               
                 SEQ ID NO: 40 Expression cassette combining SEQ ID NO: 37 (5′) and SEQ ID NO: 38 (3′), 
               
               
                 encoding the Rice rubisco promoter, cat1 intron and part of Gus plus protein, and hordeum GS1. 
               
               
                 Features shown as in SEQ ID NO: 37. Hordeum GS1 coding sequence begins after Spe1 cloning 
               
               
                 site (double underline). 
               
               
                   CTGCAG CAAAGAAACGTTATTAGTTGGTGCTTTTGGTGGTAGGAATGTAGTTTTCTG 
               
               
                 ACAAAGTCAATTACTGAATATAAAAAAAATCTGCACAGCTCTGCGTCAACAGTTGTC 
               
               
                 CAAGGGATGCCTCAAAAATCTGTGCAGATTATCAGTCGTCACGCAGAAGCAGAACA 
               
               
                 TCATGGTGTGCTAGGTCAGCTTCTTGCATTGGGCCATGAATCCGGTTGGTTGTTAATC 
               
               
                 TCTCCTCTCTTATTCTCTTATATTAAGATGCATAACTCTTTTATGTAGTCTAAAAAAA 
               
               
                 AATCCAGTGGATCGGATAGTAGTACGTCATGGTGCCATTAGGTACCGTTGAACCTAA 
               
               
                 CAGATATTTATGCATGTGTATATATATAGCTATATAGACAAAATTGATGCCGATTAT 
               
               
                 AGACCCAAAAGCAATAGGTATATATAATATAATACAGACCACACCACCAAACTAAG 
               
               
                 AATCGATCAAATAGACAAGGCATGTCTCCAAATTGTCTTAAACTATTTCCGTAGGTT 
               
               
                 CAGCCGTTCAGGAGTCGAATCAGCCTCTGCCGGCGTTTTCTTTGCACGTACGACGGA 
               
               
                 CACACATGGGCATACCATATAGCTGGTCCATGACATTAGGAGAGAGAACGTACGTG 
               
               
                 TTGACCTGTAGCTGAGATATAACAAGGTTGATTATAATATCACCAAACATGAAATCA 
               
               
                 TCCAAGGATGACCCATAACTATCACTACTATAGTACTGCATCTGGTAAAAGAAATTG 
               
               
                 TATAGACTCTATTTCGAGCACTACCACATAACGCCTGCAATGTGACACCCTACCTAT 
               
               
                 TCACTAATGTGCCTCTTCCCACACGCTTTCCACCCGTACTGCTCACAGCTTTAAGAAC 
               
               
                 CAGAACAAATGAGTAATATTAGTGTCGGTTCATGGCTAAAACCAGCACTGATGTAC 
               
               
                 ATGACCACATATGTCAAATGCTGCTTCTAGGCATGACCCGCTCTTACTAATACCTAC 
               
               
                 TCATCGCTAGAAGAATTTTCGGCTGATAAATTTTCAATTTAAGCAAGAGTTATCTGC 
               
               
                 GTTGGTTCATAACTCAAACTGATGGCCCCAACCATATTAGTGCAAATTTCACATATG 
               
               
                 ATCATAACCTTTTCATATGAAATCGGATCGAGATGAACTTTATATAAACATTGTAGC 
               
               
                 TGTCGATGATACCTACAATTTTATAGTTCACAACCTTTTTATTTCAAGTCATTTAAAT 
               
               
                 GCCCAAATAGGTGTTTCAAATCTCAGATAGAAATGTTCAAAAGTAAAAAAGGTCCC 
               
               
                 TATCATAACATAATTGATATGTAAGTGAGTTGGAAAAAGATAAGTACGTGTGAGAG 
               
               
                 AGATCGGGGATCAAATTCTGGTGTAATAATGTATGTATTTCAGTCATAAAAATTGGT 
               
               
                 AGCAGTAGTTGGGGCTCTGTATATATACCGGTAAGGATGGGATGGTAGTAGAATAA 
               
               
                 TTCTTTTTTTGTTTTTAGTTTTTTCTGGTCCAAAATTTCAAATTTGGATCCCTTACTTG 
               
               
                 TACCAACTAATATTAATGAGTGTTGAGGGTAGTAGAGGTGCAACTTTACCATAATCC 
               
               
                 CTCTGTTTCAGGTTATAAGACGTTTTGACTTTAAATTTGACCAAGTTTATGCGCAAAT 
               
               
                 ATAGTAATATTTATAATACTATATTAGTTTCATTAAATAAATAATTGAATATATTTTC 
               
               
                 ATAATAAATTTGTGTTGAGTTCAAAATATTATTAATTTTTTCTACAAACTTGGTCAAA 
               
               
                 CTTGAAGCAGTTTGACTTTGACCAAAGTCAAAACGTCTTATAACTTGAAACGGATGG 
               
               
                 ATTACTTTTTTTGTGGGGACAAGTTTACAATGTTTAATAAAGCACAATCCATCTTAAT 
               
               
                 GTTTTCAAGCTGAATATTGTAAAATTCATGGATAAACCAGCTTCTAAATGTTTAACC 
               
               
                 GGGAAAATGTCGAACGACAAATTAATATTTTTAAGTGATGGGGAGTATTAATTAAG 
               
               
                 GAGTGACAACTCAACTTTCAATATCGTACTAAACTGTGGGATTTATTTTCTAAAATTT 
               
               
                 TATACCCTGCCAATTCACGTGTTGTAGATCTTTTTTTTTCACTAACCGACACCAGGTA 
               
               
                 TATCAATTTTATTGAATATAGCAGCAAAAAGAATGTGTTGTACTTGTAAACAAAAAG 
               
               
                 CAAACTGTACATAAAAAAAAATGCACTCCTATATAATTAAGCTCATAAAGATGCTTT 
               
               
                 GCTTCGTGAGGGCCCAAGTTTTGATGACCTTTTGCTTGATCTCGAAATTAAAATTTAA 
               
               
                 GTACTGTTAAGGGAGTTCACACCACCATCAATTTTCAGCCTGAAGAAACAGTTAAAC 
               
               
                 AACGACCCCGATGACCAGTCTACTGCTCTCCACATACTAGCTGCATTATTGATCACA 
               
               
                 AAACAAAACAAAACGAAATAAAAATCAGCAGCGAGAGTGTGCAGAGAGAGACAAA 
               
               
                 GGTGATCTGGCGTGGATATCTCCCCATCCATCCTCACCCGCGCTGCCCATCACTCGC 
               
               
                 CGCCGCATACTCCATCATGTGGAGAGAGGAAGACGAGGACCACAGCCAGAGCCCGG 
               
               
                 GTCGAGATGCCACCACGGCCACAACCCACGAGCCCGGCGCGACACCACCGCGCGCG 
               
               
                 CGTGAGCCAGCCACAAACGCCCGCGGATAGGCGCGCGCACGCCGGCCAATCCTACC 
               
               
                 ACATCCCCGGCCTCCGCGGCTCGCGAGCGCCGCTGCCATCCGATCCGCTGAGTTTTG 
               
               
                 GCTATTTATACGTACCGCGGGAGCCTGTGTGCAGAGCAGTGCATCTCAAGAAGTACT 
               
               
                 CGAGCAAAGAAGGAGAGAGCTTGGTGAGCTGCAG CC     ATG     G TAGATCTGAGG   GTAAA     
               
               
                 
                   
                     TTTCTAGTTTTTCTCCTTCATTTTCTTGGTTAGGACCCTTTTCTCTTTTTATTTTT 
                   
                 
               
               
                 
                   
                     TTGAGCTTTGATCTTTCTTTAAACTGATCTATTTTTTAATTGATTGGTTATGGTG 
                   
                 
               
               
                 
                   
                     TAAATATTACATAGCTTTAACTGATAATCTGATTACTTTATTTCGTGTGTCTATG 
                   
                 
               
               
                     ATGATGATGATAGTTACAG   AACCGACGAACTAGTGCGCAGGCGGTTGTGCAGGCG 
               
               
                 ATGCAGTGCCAGGTGGGGGTGAGGGGCAGGACGGCCGTCCCGGCGAGGCAGCCCG 
               
               
                 CGGGCAGGGTGTGGGGCGTCAGGAGGGCCGCCCGCGCCACCTCCGGGTTCAAGGTG 
               
               
                 CTGGCGCTCGGCCCGGAGACCACCGGGGTCATCCAGAGGATGCAGCAGCTGCTCGA 
               
               
                 CATGGACACCACGCCCTTCACCGACAAGATCATCGCCGAGTACATCTGGGTTGGAG 
               
               
                 GATCTGGAATTGACCTCAGAAGCAAATCAAGGACGATTTCGAAGCCAGTGGAGGAC 
               
               
                 CCGTCAGAGCTGCCGAAATGGAACTACGACGGATCGAGCACGGGGCAGGCTCCTGG 
               
               
                 GGAAGACAGTGAAGTCATCCTATACCCACAGGCCATATTCAAGGACCCATTCCGAG 
               
               
                 GAGGCAACAACATACTGGTTATCTGTGACACCTACACACCACAGGGGGAACCCATC 
               
               
                 CCTACTAACAAACGCCACATGGCTGCACAAATCTTCAGTGACCCCAAGGTCACTTCA 
               
               
                 CAAGTGCCATGGTTCGGAATCGAACAGGAGTACACTCTGATGCAGAGGGATGTGAA 
               
               
                 CTGGCCTCTTGGCTGGCCTGTTGGAGGGTACCCTGGCCCCCAGGGTCCATACTACTG 
               
               
                 CGCCGTAGGATCAGACAAGTCATTTGGCCGTGACATATCAGATGCTCACTACAAGGC 
               
               
                 GTGCCTTTACGCTGGAATTGAAATCAGTGGAACAAACGGGGAGGTCATGCCTGGTC 
               
               
                 AGTGGGAGTACCAGGTTGGACCCAGCGTTGGTATTGATGCAGGAGACCACATATGG 
               
               
                 GCTTCCAGATACATTCTCGAGAGAATCACGGAGCAAGCTGGTGTGGTGCTCACCCTT 
               
               
                 GACCCAAAACCAATCCAGGGTGACTGGAACGGAGCTGGCTGCCACACAAACTACAG 
               
               
                 CACATTGAGCATGCGCGAGGATGGAGGTTTCGACGTGATCAAGAAGGCAATCCTGA 
               
               
                 ACCTTTCACTTCGCCATGACTTGCACATAGCCGCATATGGTGAAGGAAACGAGCGGA 
               
               
                 GGTTGACAGGGCTACACGAGACAGCTAGCATATCAGACTTCTCATGGGGTGTGGCG 
               
               
                 AACCGTGGCTGCTCTATTCGTGTGGGGCGAGACACCGAGGCGAAGGGCAAAGGATA 
               
               
                 CCTGGAGGACCGTCGCCCGGCCTCCAACATGGACCCGTACACCGTGACGGCGCTGC 
               
               
                 TGGCCGAGACCACGATCCTGTGGGAGCCGACCCTCGAGGCGGAGGCCCTCGCTGCC 
               
               
                 AAGAAGCTGGCGCTGAAGGTATGA 
               
               
                   
               
               
                 SEQ ID NO: 41 Amino acid sequence of translation product of SEQ ID NO: 40. Amino- 
               
               
                 terminal bold residues from Gusplus and Spe1 cloning site (intron removed) 
               
               
                   MVDLRNRRTS AQAVVQAMQCQVGVRGRTAVPARQPAGRVWGVRRAARATSGFKVL 
               
               
                 ALGPETTGVIQRMQQLLDMDTTPFTDKIIAEYIWVGGSGIDLRSKSRTISKPVEDPSELPK 
               
               
                 WNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPQGEPIPTNKRHMAAQ 
               
               
                 IFSDPKVTSQVPWFGIEQEYTLMQRDVNWPLGWPVGGYPGPQGPYYCAVGSDKSFGRD 
               
               
                 ISDAHYKACLYAGIEISGTNGEVMPGQWEYQVGPSVGIDAGDHIWASRYILERITEQAG 
               
               
                 VVLTLDPKPIQGDWNGAGCHTNYSTLSMREDGGFDVIKKAILNLSLRHDLHIAAYGEGN 
               
               
                 ERRLTGLHETASISDFSWGVANRGCSIRVGRDTEAKGKGYLEDRRPASNMDPYTVTALL 
               
               
                 AETTILWEPTLEAEALAAKKLALKV 
               
               
                   
               
               
                 SEQ ID NO: 42 Maize ubil promoter: 5′ UTR intron shown in italics, TATA box at −30 is 
               
               
                 underlined, 5′ and 3′ Pst1 cloning sites in bold 
               
               
                   CTGCAG TGCAGCGTGACCCGGTCGTGCCCCTCTCTAGAGATAATGAGCATTGCATGT 
               
               
                 CTAAGTTATAAAAAATTACCACATATTTTTTTTGTCACACTTGTTTGAAGTGCAGTTT 
               
               
                 ATCTATCTTTATACATATATTTAAACTTTACTCTACGAATAATATAATCTATAGTACT 
               
               
                 ACAATAATATCAGTGTTTTAGAGAATCATATAAATGAACAGTTAGACATGGTCTAAA 
               
               
                 GGACAATTGAGTATTTTGACAACAGGACTCTACAGTTTTATCTTTTTAGTGTGCATGT 
               
               
                 GTTCTCCTTTTTTTTTGCAAATAGCTTCACCTATATAATACTTCATCCATTTTATTAGT 
               
               
                 ACATCCATTTAGGGTTTAGGGTTAATGGTTTTTATAGACTAATTTTTTTAGTACATCT 
               
               
                 ATTTTATTCTATTTTAGCCTCTAAATTAAGAAAACTAAAACTCTATTTTAGTTTTTTTA 
               
               
                 TTTAATAATTTAGATATAAAATAGAATAAAATAAAGTGACTAAAAATTAAACAAAT 
               
               
                 ACCCTTTAAGAAATTAAAAAAACTAAGGAAACATTTTTCTTGTTTCGAGTAGATAAT 
               
               
                 GCCAGCCTGTTAAACGCCGTCGACGAGTCTAACGGACACCAACCAGCGAACCAGCA 
               
               
                 GCGTCGCGTCGGGCCAAGCGAAGCAGACGGCACGGCATCTCTGTCGCTGCCTCTGG 
               
               
                 ACCCCTCTCGAGAGTTCCGCTCCACCGTTGGACTTGCTCCGCTGTCGGCATCCAGAA 
               
               
                 ATTGCGTGGCGGAGCGGCAGACGTGAGCCGGCACGGCAGGCGGCCTCCTCCTCCTC 
               
               
                 TCACGGCACGGCAGCTACGGGGGATTCCTTTCCCACCGCTCCTTCGCTTTCCCTTCCT 
               
               
                 CGCCCGCCG TAATAAATA GACACCCCCTCCACACCCTCTTTCCCCAACCTCGTGTTG 
               
               
                 TTCGGAGCGCACACACACACAACCAGATCTCCCCCAAATCCACCCGTCGGCACCTCC 
               
               
                 GCTTCAAGGTACGCCGCTCGTCCTCCCCCCCCCCCCCTCTCTACCTTCTCTAGATCGG 
               
               
                 CGTTCCGGTCCATGGTTAGGGCCCGGTAGTTCTACTTCTGTTCATGTTTGTGTTAGAT 
               
               
                 CCGTGTTTGTGTTAGATCCGTGCTGCTAGCGTTCGTACACGGATGCGACCTGTACGT 
               
               
                 CAGACACGTTCTGATTGCTAACTTGCCAGTGTTTCTCTTTGGGGAATCCTGGGATGG 
               
               
                 CTCTAGCCGTTCCGCAGACGGGATCGATTTCATGATTTTTTTTGTTTCGTTGCATAGG 
               
               
                 GTTTGGTTTGCCCTTTTCCTTTATTTCAATATATGCCGTGCACTTGTTTGTCGGGTCAT 
               
               
                 CTTTTCATGCTTTTTTTTGTCTTGGTTGTGATGATGTGGTCTGGTTGGGCGGTCGTTCT 
               
               
                 AGATCGGAGTAGAATTCTGTTTCAAACTACCTGGTGGATTTATTAATTTTGGATCTGT 
               
               
                 ATGTGTGTGCCATACATATTCATAGTTACGAATTGAAGATGATGGATGGAAATATCG 
               
               
                 ATCTAGGATAGGTATACATGTTGATGCGGGTTTTACTGATGCATATACAGAGATGCT 
               
               
                 TTTTGTTCGCTTGGTTGTGATGATGTGGTGTGGTTGGGCGGTCGTTCATTCGTTCTAG 
               
               
                 ATCGGAGTAGAATACTGTTTCAAACTACCTGGTGTATTTATTAATTTTGGAACTGTAT 
               
               
                 GTGTGTGTCATACATCTTCATAGTTACGAGTTTAAGATGGATGGAAATATCGATCTA 
               
               
                 GGATAGGTATACATGTTGATGTGGGTTTTACTGATGCATATACATGATGGCATATGC 
               
               
                 AGCATCTATTCATATGCTCTAACCTTGAGTACCTATCTATTATAATAAACAAGTATGT 
               
               
                 TTTATAATTATTTTGATCTTGATATACTTGGATGATGGCATATGCAGCAGCTATATGT 
               
               
                 GGATTTTTTTAGCCCTGCCTTCATACGCTATTTATTTGCTTGGTACTGTTTCTTTTGTC 
               
               
                 GATGCTCACCCTGTTGTTTGGTGTTACTT CTGCAG   
               
               
                   
               
               
                 SEQ ID NO: 43 Hordeum GPT DNA coding sequence, including targeting sequence coding 
               
               
                 domain 
               
               
                   ATG GCATCCGCCCCCGCCTCCGCCTCCGCGGCCCTCTCCACCGCCGCCCCCGCCGAC 
               
               
                 AACGGGGCCGCCAAGCCCACGGAGCAGCGGCCGGTACAGGTGGCTAAGCGATTGG 
               
               
                 AGAAGTTCAAAACAACAATTTTCACACAGATGAGCATGCTCGCAGTGAAGCATGGA 
               
               
                 GCAATAAACCTTGGACAGGGGTTTCCCAATTTTGATGGCCCTGACTTTGTCAAAGAT 
               
               
                 GCTGCTATTGAGGCTATCAAAGCTGGAAAGAATCAGTATGCAAGAGGATATGGTGT 
               
               
                 GCCTGAATTGAACTCAGCTGTTGCTGAGAGATTTCTCAAGGACAGTGGATTGCACAT 
               
               
                 CGATCCTGATAAGGAAGTTACTGTTACATCTGGGTGCACAGAAGCAATAGCTGCAA 
               
               
                 CGATATTGGGTCTGATCAACCCTGGGGATGAAGTCATACTGTTTGCTCCATTCTATG 
               
               
                 ATTCTTATGAGGCTACACTGTCCATGGCTGGTGCGAATGTCAAAGCCATTACACTCC 
               
               
                 GCCCTCCGGACTTTGCAGTCCCTCTTGAAGAGCTAAAGGCTGCAGTCTCGAAGAATA 
               
               
                 CCAGAGCAATAATGATTAATACACCTCACAACCCTACCGGGAAAATGTTCACAAGG 
               
               
                 GAGGAACTTGAGTTCATTGCTGATCTCTGCAAGGAAAATGACGTGTTGCTCTTTGCC 
               
               
                 GATGAGGTCTACGACAAGCTGGCGTTTGAGGCGGATCACATATCAATGGCTTCTATT 
               
               
                 CCTGGCATGTATGAGAGGACCGTCACTATGAACTCCCTGGGGAAGACGTTCTCCTTG 
               
               
                 ACCGGATGGAAGATCGGCTGGGCGATAGCACCACCGCACCTGACATGGGGCGTAAG 
               
               
                 GCAGGCACACTCCTTCCTCACATTCGCCACCTCCACGCCGATGCAATCAGCAGCGGC 
               
               
                 GGCGGCCCTGAGAGCACCGGACAGCTACTTTGAGGAGCTGAAGAGGGACTACGGCG 
               
               
                 CAAAGAAAGCGCTGCTGGTGGACGGGCTCAAGGCGGCGGGCTTCATCGTCTACCCT 
               
               
                 TCGAGCGGAACCTACTTCATCATGGTCGACCACACCCCGTTCGGGTTCGACAACGAC 
               
               
                 GTCGAGTTCTGCGAGTACTTGATCCGCGAGGTCGGCGTCGTGGCCATCCCGCCAAGC 
               
               
                 GTGTTCTACCTGAACCCGGAGGACGGGAAGAACCTGGTGAGGTTCACCTTCTGCAA 
               
               
                 GGACGACGACACGCTAAGGGCGGCGGTGGACAGGATGAAGGCCAAGCTCAGGAAG 
               
               
                 AAATGA 
               
               
                   
               
               
                 SEQ ID NO: 44 Hordeum GPT amino acid sequence, including putative targeting sequence (in 
               
               
                 italics). 
               
               
                   MASAPASASAALSTAAPADNGAAKPTEQRPVQ VAKRLEKFKTTIFTQMSMLAVKHGAINLG 
               
               
                 QGFPNFDGPDFVKDAAIEAIKAGKNQYARGYGVPELNSAVAERFLKDSGLHIDPDKEVT 
               
               
                 VTSGCTEAIAATILGLINPGDEVILFAPFYDSYEATLSMAGANVKAITLRPPDFAVPLEEL 
               
               
                 KAAVSKNTRAIMINTPHNPTGKMFTREELEFIADLCKENDVLLFADEVYDKLAFEADHIS 
               
               
                 MASIPGMYERTVTMNSLGKTFSLTGWKIGWAIAPPHLTWGVRQAHSFLTFATSTPMQS 
               
               
                 AAAAALRAPDSYFEELKRDYGAKKALLVDGLKAAGFIVYPSSGTYFIMVDHTPFGFDN 
               
               
                 DVEFCEYLIREVGVVAIPPSVFYLNPEDGKNLVRFTFCKDDDTLRAAVDRMKAKLRKK 
               
               
                   
               
               
                 SEQ ID NO: 45 Tomato rubisco small subunit (rbcS3C) promoter +  Arabidopsis  GS1 DNA 
               
               
                 coding sequence; Nco1/Affillsplice site shown in bold, ATG start of GS1 underlined. 
               
               
                 GTTTGAATCCTCCTTAAAGTTTTTCTCTGGAGAAACTGTAGTAATTTTACTTTGTTGT 
               
               
                 GTTCCCTTCATCTTTTGAATTAATGGCATTTGTTTTAATACTAATCTGCTTCTGAAACT 
               
               
                 TGTAATGTATGTATATCAGTTTCTTATAATTTATCCAAGTAATATCTTCCATTCTCTAT 
               
               
                 GCAATTGCCTGCATAAGCTCGACAAAAGAGTACATCAACCCCTCCTCCTCTGGACTA 
               
               
                 CTCTAGCTAAACTTGAATTTCCCCTTAAGATTATGAAATTGATATATCCTTAACAAAC 
               
               
                 GACTCCTTCTGTTGGAAAATGTAGTACTTGTCTTTCTTCTTTTGGGTATATATAGTTT 
               
               
                 ATATACACCATACTATGTACAACATCCAAGTAGAGTGAAATGGATACATGTACAAG 
               
               
                 ACTTATTTGATTGATTGATGACTTGAGTTGCCTTAGGAGTAACAAATTCTTAGGTCA 
               
               
                 ATAAATCGTTGATTTGAAATTAATCTCTCTGTCTTAGACAGATAGGAATTATGACTTC 
               
               
                 CAATGGTCCAGAAAGCAAAGTTCGCACTGAGGGTATACTTGGAATTGAGACTTGCA 
               
               
                 CAGGTCCAGAAACCAAAGTTCCCATCGAGCTCTAAAATCACATCTTTGGAATGAAAT 
               
               
                 TCAATTAGAGATAAGTTGCTTCATAGCATAGGTAAAATGGAAGATGTGAAGTAACC 
               
               
                 TGCAATAATCAGTGAAATGACATTAATACACTAAATACTTCATATGTAATTATCCTT 
               
               
                 TCCAGGTTAACAATACTCTATAAAGTAAGAATTATCAGAAATGGGCTCATCAAACTT 
               
               
                 TTGTACTATGTATTTCATATAAGGAAGTATAACTATACATAAGTGTATACACAACTT 
               
               
                 TATTCCTATTTTGTAAAGGTGGAGAGACTGTTTTCGATGGATCTAAAGCAATATGTC 
               
               
                 TATAAAATGCATTGATATAATAATTATCTGAGAAAATCCAGAATTGGCGTTGGATTA 
               
               
                 TTTCAGCCAAATAGAAGTTTGTACCATACTTGTTGATTCCTTCTAAGTTAAGGTGAA 
               
               
                 GTATCATTCATAAACAGTTTTCCCCAAAGTACTACTCACCAAGTTTCCCTTTGTAGAA 
               
               
                 TTAACAGTTCAAATATATGGCGCAGAAATTACTCTATGCCCAAAACCAAACGAGAA 
               
               
                 AGAAACAAAATACAGGGGTTGCAGACTTTATTTTCGTGTTAGGGTGTGTTTTTTCAT 
               
               
                 GTAATTAATCAAAAAATATTATGACAAAAACATTTATACATATTTTTACTCAACACT 
               
               
                 CTGGGTATCAGGGTGGGTTGTGTTCGACAATCAATATGGAAAGGAAGTATTTTCCTT 
               
               
                 ATTTTTTTAGTTAATATTTTCAGTTATACCAAACATACCTTGTGATATTATTTTTAAA 
               
               
                 AATGAAAAACTCGTCAGAAAGAAAAAGCAAAAGCAACAAAAAAATTGCAAGTATT 
               
               
                 TTTTAAAAAAGAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGAGATAA 
               
               
                 GGACGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGAACCAC 
               
               
                 AAAATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTCCGTTA 
               
               
                 GATAGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTAACCAA 
               
               
                 TTATTTCAGC ACC     ATG     T CTCTGCTCTCAGATCTCGTTAACCTCAACCTCACCGATGC 
               
               
                 CACCGGGAAAATCATCGCCGAATACATATGGATCGGTGGATCTGGAATGGATATCA 
               
               
                 GAAGCAAAGCCAGGACACTACCAGGACCAGTGACTGATCCATCAAAGCTTCCCAAG 
               
               
                 TGGAACTACGACGGATCCAGCACCGGTCAGGCTGCTGGAGAAGACAGTGAAGTCAT 
               
               
                 TCTATACCCTCAGGCAATATTCAAGGATCCCTTCAGGAAAGGCAACAACATCCTGGT 
               
               
                 GATGTGTGATGCTTACACACCAGCTGGTGATCCTATTCCAACCAACAAGAGGCACAA 
               
               
                 CGCTGCTAAGATCTTCAGCCACCCCGACGTTGCCAAGGAGGAGCCTTGGTATGGGAT 
               
               
                 TGAGCAAGAATACACTTTGATGCAAAAGGATGTGAACTGGCCAATTGGTTGGCCTGT 
               
               
                 TGGTGGCTACCCTGGCCCTCAGGGACCTTACTACTGTGGTGTGGGAGCTGACAAAGC 
               
               
                 CATTGGTCGTGACATTGTGGATGCTCACTACAAGGCCTGTCTTTACGCCGGTATTGG 
               
               
                 TATTTCTGGTATCAATGGAGAAGTCATGCCAGGCCAGTGGGAGTTCCAAGTCGGCCC 
               
               
                 TGTTGAGGGTATTAGTTCTGGTGATCAAGTCTGGGTTGCTCGATACCTTCTCGAGAG 
               
               
                 GATCACTGAGATCTCTGGTGTAATTGTCAGCTTCGACCCGAAACCAGTCCCGGGTGA 
               
               
                 CTGGAATGGAGCTGGAGCTCACTGCAACTACAGCACTAAGACAATGAGAAACGATG 
               
               
                 GAGGATTAGAAGTGATCAAGAAAGCGATAGGGAAGCTTCAGCTGAAACACAAAGA 
               
               
                 ACACATTGCTGCTTACGGTGAAGGAAACGAGCGTCGTCTCACTGGAAAGCACGAAA 
               
               
                 CCGCAGACATCAACACATTCTCTTGGGGAGTCGCGAACCGTGGAGCGTCAGTGAGA 
               
               
                 GTGGGACGTGACACAGAGAAGGAAGGTAAAGGGTACTTCGAAGACAGAAGGCCAG 
               
               
                 CTTCTAACATGGATCCTTACGTTGTCACCTCCATGATCGCTGAGACGACCATACTCG 
               
               
                 GTTGA 
               
               
                   
               
               
                 SEQ ID NO: 46 Tomato rubisco small subunit promoter (rbcS3C) +  Zea Mays  AAT [GPT] 
               
               
                 ccatgg is Nco1 cloning site 3′ end of promoter into vector 
               
               
                 actagt is Spe1 cloning site to insert gene to promoter + intron 
               
               
                 ggtacc is Kpn1 cloning site 5′ end of promoter to vector 
               
               
                 Sequence in double underline is the cat1 intron from the Cambia 1305.1 vector; first 10 amino 
               
               
                 acids are from GUSplus enzyme and cloning sites in 1305.1 vector 
               
               
                            GTTTGAATCCTCCTTAAAGTTTTTCTCTGGAGAAACTGTAGTAATTTTACTT 
               
               
                 TGTTGTGTTCCCTTCATCTTTTGAATTAATGGCATTTGTTTTAATACTAATCTGCTTCT 
               
               
                 GAAACTTGTAATGTATGTATATCAGTTTCTTATAATTTATCCAAGTAATATCTTCCAT 
               
               
                 TCTCTATGCAATTGCCTGCATAAGCTCGACAAAAGAGTACATCAACCCCTCCTCCTC 
               
               
                 TGGACTACTCTAGCTAAACTTGAATTTCCCCTTAAGATTATGAAATTGATATATCCTT 
               
               
                 AACAAACGACTCCTTCTGTTGGAAAATGTAGTACTTGTCTTTCTTCTTTTGGGTATAT 
               
               
                 ATAGTTTATATACACCATACTATGTACAACATCCAAGTAGAGTGAAATGGATACATG 
               
               
                 TACAAGACTTATTTGATTGATTGATGACTTGAGTTGCCTTAGGAGTAACAAATTCTT 
               
               
                 AGGTCAATAAATCGTTGATTTGAAATTAATCTCTCTGTCTTAGACAGATAGGAATTA 
               
               
                 TGACTTCCAATGGTCCAGAAAGCAAAGTTCGCACTGAGGGTATACTTGGAATTGAG 
               
               
                 ACTTGCACAGGTCCAGAAACCAAAGTTCCCATCGAGCTCTAAAATCACATCTTTGGA 
               
               
                 ATGAAATTCAATTAGAGATAAGTTGCTTCATAGCATAGGTAAAATGGAAGATGTGA 
               
               
                 AGTAACCTGCAATAATCAGTGAAATGACATTAATACACTAAATACTTCATATGTAAT 
               
               
                 TATCCTTTCCAGGTTAACAATACTCTATAAAGTAAGAATTATCAGAAATGGGCTCAT 
               
               
                 CAAACTTTTGTACTATGTATTTCATATAAGGAAGTATAACTATACATAAGTGTATAC 
               
               
                 ACAACTTTATTCCTATTTTGTAAAGGTGGAGAGACTGTTTTCGATGGATCTAAAGCA 
               
               
                 ATATGTCTATAAAATGCATTGATATAATAATTATCTGAGAAAATCCAGAATTGGCGT 
               
               
                 TGGATTATTTCAGCCAAATAGAAGTTTGTACCATACTTGTTGATTCCTTCTAAGTTAA 
               
               
                 GGTGAAGTATCATTCATAAACAGTTTTCCCCAAAGTACTACTCACCAAGTTTCCCTTT 
               
               
                 GTAGAATTAACAGTTCAAATATATGGCGCAGAAATTACTCTATGCCCAAAACCAAA 
               
               
                 CGAGAAAGAAACAAAATACAGGGGTTGCAGACTTTATTTTCGTGTTAGGGTGTGTTT 
               
               
                 TTTCATGTAATTAATCAAAAAATATTATGACAAAAACATTTATACATATTTTTACTCA 
               
               
                 ACACTCTGGGTATCAGGGTGGGTTGTGTTCGACAATCAATATGGAAAGGAAGTATTT 
               
               
                 TCCTTATTTTTTTAGTTAATATTTTCAGTTATACCAAACATACCTTGTGATATTATTTT 
               
               
                 TAAAAATGAAAAACTCGTCAGAAAGAAAAAGCAAAAGCAACAAAAAAATTGCAAG 
               
               
                 TATTTTTTAAAAAAGAAAAAAAAAAACATATCTTGTTTGTCAGTATGGGAAGTTTGA 
               
               
                 GATAAGGACGAGTGAGGGGTTAAAATTCAGTGGCCATTGATTTTGTAATGCCAAGA 
               
               
                 ACCACAAAATCCAATGGTTACCATTCCTGTAAGATGAGGTTTGCTAACTCTTTTTGTC 
               
               
                 CGTTAGATAGGAAGCCTTATCACTATATATACAAGGCGTCCTAATAACCTCTTAGTA 
               
               
                 ACCAATTATTTCAGCA          TAGATCTGAGGGTAAATTTCTAGTTTTTCTCCTTCA 
               
               
                 TTTTCTTGGTTAGGACCCTTTTCTCTTTTTATTTTTTTGAGCTTTGATCTTTCTTTAAAC 
               
               
                 TGATCTATTTTTTAATTGATTGGTTATGGTGTAAATATTACATAGCTTTAACTGATAA 
               
               
                   TCTGATTACTTTATTTCGTGTGTCTATGATGATGATGATAGTTACAGAA CCGACGA A   
               
               
                   CTAGT ATGAATCTGGCCGCCTTTTCCTCCACCCTTGCCACGCTCCCCTGGTATGAGA 
               
               
                 TGCCATCAATAAATTCCTCCGCAACTTTCTCGTCCTCACTGCTCCGCCGCTCGCTCTG 
               
               
                 CGCGTCGCTCCGGACGATCTCCCACATGGCCTCCGCCGCCGCCCCCACCTCCGCGCC 
               
               
                 CGTCGCCACCACCGAGAACGGCGCCGCGAAGGCGATAGAGCAGCGGCCCGTGCAG 
               
               
                 GTCGCAGAGCGGCTGGAAAAGTTCAAGACAACAATTTTCACTCAGATGAGCATGCT 
               
               
                 TGCCATCAAGCATGGAGCAATAAACCTTGGCCAGGGCTTTCCGAATTTTGATGGCCC 
               
               
                 AGACTTTGTGAAAGAGGCCGCAATTCAAGCTATCAATGCTGGGAAGAATCAGTACG 
               
               
                 CAAGAGGGTTTGGTGTGCCTGAACTGAACTCGGCTATCGCTGAAAGGTTCCTGAAGG 
               
               
                 ACAGTGGATTGCAAGTTGACCCTGACAAGGAAGTCACTGTTACATCTGGATGCACTG 
               
               
                 AGGCAATAGCTGCAACCATACTAGGTCTGATCAATCCTGGCGACGAGGTGATACTGT 
               
               
                 TCGCCCCATTCTACGATTCATACGAGGCTACACTGTCGATGGCCGGTGCCAACGTGA 
               
               
                 AGGCCATTACCCTCCGCGCTCCAGATTTCGCGGTCCCGCTTGAGGAGCTGGAGGCTG 
               
               
                 CAGTCTCCAAGGACACGAAAGCGATAATGATAAACACGCCGCACAACCCAACCGGG 
               
               
                 AAAATGTTCACCAGGGAGGAGCTCGAATCCATCGCCGCCCTCTGCAAGGAAAACGA 
               
               
                 CGTTTTGCTGTTCTCAGATGAGGTCTATGACAAGCTGGTGTTTGAGGCTGACCACAT 
               
               
                 ATCCATGGCTTCTATCCCGGGCATGTACGAGAGGACGGTGACCATGAACTCTCTGGG 
               
               
                 GAAGACGTTCTCTCTTACAGGATGGAAGATCGGGTGGGCAATCGCGCCGCCGCACC 
               
               
                 TGACATGGGGCCTCAGGCAGGCGCACTCGTTCCTGACGTTCGCCACCTGCACACCGA 
               
               
                 TGCAGGCGGCGGCCGCGGCGGCTCTGAGGGCACCGGACAGCTACTACGACGAGCTG 
               
               
                 AAGAGGGACTACAGCGCGAAGAAGGCTATCCTGCTGGAAGGACTCGAAGCCGCAG 
               
               
                 GGTTCATCGTCTACCCATCGAGTGGGACATACTACATCATGGTCGACCACACCCCGT 
               
               
                 TCGGTTTCGACAGCGACGTAGAGTTCTGCGAGTACTTGATCCGCGAAGTCGGCGTCT 
               
               
                 GCGCTATACCGCCCAGCGTGTTCTACCTCGACCCCGAAGAGGGAAAGAAATTGGTG 
               
               
                 AGGTTCACCTTCAGCAAGGACGAAGGCACGCTGCGGGCCGCGGTCGAGAGGTTGAA 
               
               
                 GGCGAAGCTCAGGAGGAAATGA 
               
               
                   
               
               
                 SEQ ID NO: 47  Zea mays  GPT translation product (of SEQ ID NO: 46) 
               
               
                 Italicized bold amino acids are from gus plus sequence that remain after the intron is removed. 
               
               
                            MNLAAFSSTLATLPWYEMPSINSSATFSSSLLRRSLCASLRTISHMASAA 
               
               
                 APTSAPVATTENGAAKAIEQRPVQVAERLEKFKTTIFTQMSMLAIKHGAINLGQGFPNFD 
               
               
                 GPDFVKEAAIQAINAGKNQYARGFGVPELNSAIAERFLKDSGLQVDPDKEVTVTSGCTE 
               
               
                 AIAATILGLINPGDEVILFAPFYDSYEATLSMAGANVKAITLRAPDFAVPLEELEAAVSKD 
               
               
                 TKAIMINTPHNPTGKMFTREELESIAALCKENDVLLFSDEVYDKLVFEADHISMASIPGM 
               
               
                 YERTVTMNSLGKTFSLTGWKIGWAIAPPHLTWGLRQAHSFLTFATCTPMQAAAAAALR 
               
               
                 APDSYYDELKRDYSAKKAILLEGLEAAGFIVYPSSGTYYIMVDHTPFGFDSDVEFCEYLI 
               
               
                 REVGVCAIPPSVFYLDPEEGKKLVRFTFSKDEGTLRAAVERLKAKLRRK- 
               
               
                   
               
               
                 SEQ ID NO: 48  Chlorella  GPT amino acid sequence 
               
               
                 MAAAAAGGDGPSAARRFNSTFSSLPTTIFEQMSLLAAKHQSTNLGQGFPDNELEGPESM 
               
               
                 KKVMISLYEHSNQYPPLMGLPELRQAVAAHSARHAGIPVDWQAETLVTVGATEALAAA 
               
               
                 FLGLLDAGDEVIFFEPLYDSYVPMARRAGAIPRIVQLYPPAWSIDAAELEAAFSPQTKLL 
               
               
                 VLNTPHNPTGKVFGAEELQLIADLCQKHDCLCLLDEVYEHLVFPGTRHTSLQSLPGMRE 
               
               
                 RCLRVGWLSGPHDLLAAVTKAHQFLIFTVPSALQRAVAYGLEQEEAFCCGLGAALSKK 
               
               
                 RQLLEGQLAEIGFAVLPAQGTYFLVADFAGLLPAGSSEDDVQFCHRLTVEAGVTLIPVSA 
               
               
                 FYADRAATPRTLVRFVFCKTDEKLNTACGKLRTYFGRQ