Abstract:
Provided herein are methods for miRNA profiling for the diagnosis, prognosis, and management of melanoma and differentiation of melanoma from nevi.

Description:
FIELD OF THE INVENTION 
     The invention generally relates to miRNA profiling for the diagnosis, prognosis, and management of melanoma and differentiation of melanoma from nevi. 
     BACKGROUND OF THE INVENTION 
     The following discussion of the background of the invention is merely provided to aid the reader in understanding the invention and is not admitted to describe or constitute prior art to the present invention. 
     Skin cancer is the most common of all cancers, afflicting more than a million Americans each year, a number that is rising rapidly. It is also the easiest to cure, if diagnosed and treated early. If allowed to progress to the point where it spreads to other sites (metastasizes), the prognosis (forecast) is very poor. More than 8,000 melanoma deaths now occur per year. 
     Melanoma most often appears as an asymmetrical, irregularly bordered, multicolored or tan/brown spot or growth that continues to increase in size over time. It may begin as a flat spot and become more elevated. In rare instances, it may not be pigmented. 
     Dysplastic nevi (atypical moles) are unusual or benign moles that may resemble melanoma. People who have them are at increased risk of developing single or multiple melanomas. The higher the number of these moles someone has, the higher the risk; those who have 10 or more have 12 times the risk of developing melanoma compared to the general population. Dysplastic nevi are found significantly more often in melanoma patients than in the general population. 
     Melanoma is distinguished from nevi, other forms of cancer, and normal skin on the basis of clinical presentation and histopathological examination of a skin biopsy, usually a formalin fixed, paraffin embedded (FFPE) sample. Considerable expertise is required to reliably distinguish between nevi and melanoma. 
     This application describes novel microRNA biomarkers with microRNA array and RT-PCR to better characterize dysplastic nevi, malignant melanoma and metastatic melanoma, miRNA can therefore serve as an adjunct to histopathology for correct classification of melanoma, nevi and other conditions, especially where there is doubt as to the diagnosis. 
     SUMMARY OF THE INVENTION 
     The present invention is based on the discovery that melanoma can be distinguished from nevi by measuring changes in the levels of as little as two miRNAs. 
     In one aspect, the invention provides a method for differentially diagnosing melanoma from nevi, by (a) measuring the level of two or more miRNAs selected from the group consisting of miR-132, miR-150, miR-339-5p, miR-15b, miR-342-3p, miR-572, miR-155, miR-425, miR-1202, miR-1268, HBII-382_s, miR-1225-5p, miR-30c, miR-106b-star, miR-125a-5p, mgU6-53B, miR-25, miR-149-star, miR-939, miR-92b-star, miR-500-star, miR-22, HBII-142_x, miR-181b, HBII-142, U38B, miR-663, miR-1224-5p, miR-23a, HBII-85-6_x, miR-1207-5p, miR-1301, miR-1228-star, miR-345, miR-30a-star, ENSG00000199411, ENSG00000202327, miR-92a, miR-127-3p, HBII-85-26, miR-1308, miR-31, miR-921, miR-146b-5p, miR-768-3p, miR-708, miR-139-5p, ACA24_x, miR-501-3p, miR-502-3p, miR-923, and miR-191; and (h) diagnosing the skin sample as containing melanoma when a difference in the level of the two or more miRNAs compared to a reference level indicates melanoma in the sample. The level of two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, or more, miRNAs are measured. 
     In specific embodiments, the miRNAs to be measured include the following combinations (i) miR-150 and miR-149-star; (ii) miR-150, miR-149-star and miR-1308; (iii) miR-150, miR-149-star, miR-1308, and miR-191; (iv) miR-150, miR-149-star, miR-1308, miR-191, and miR-1228-star; (v) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, and ENSG00000199411; (vi) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, ENSG00000199411, and miR-1268; (vii) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, ENSG00000199411, miR-1268, and miR-923; (viii) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, ENSG00000199411, miR-1268, miR-923, and miR-23a; (ix) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, ENSG00000199411, miR-1268, miR-923, miR-23a, and miR-132; (x) miR-150, miR-149-star, miR-1308, miR-191., miR-1228-star, ENSG00000199411, miR-1268, miR-923, miR-23a, miR-132, and miR-1207.5p; (xi) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, ENSG00000199411, miR-1268, miR-923, miR-23a, miR-132, miR-1207.5p, and miR-342.3p; (xii) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, ENSG00000199411, miR-1268, miR-923, miR-23a, miR-132, miR-1207.5p, miR-342.3p, and U38B; (xiii) miR-150, miR-149-star, miR-1308, miR-191, miR-1228-star, ENSG00000199411, miR-1268, miR-923, miR-23a, miR-132, miR-1207.5p, miR-342.3p, U38B, and miR-155. 
     In specific embodiments, melanoma is diagnosed in the skin sample by alterations in the level of an miRNA compared to a reference level, with the following alterations observed in at least two miRNA selected from the group consisting of: (i) miR-150 increase; (ii) miR-149-star decrease; (iii) miR-1308 decrease; (iv) miR-191 increase; (v) miR-1228-star decrease; (vi) ENSG00000199411_s decrease; (vii) miR-1268 decrease; (viii) miR-923 decrease; (ix) miR-23a increase; (x) miR-132 increase; (xi) miR-1207.5p decrease; (xii) miR-342.3p increase; (xiii) U38B decrease; and (xiv) miR-155 increase. 
     In one aspect, the invention provides a method for differentially diagnosing melanoma from nevi, by (a) measuring the level of two or more miRNAs selected from the group consisting of: miR-1268, miR-1228-star, miR-92b-star, miR-155, miR-345, miR-425, miR-132, miR-1207-5p, miR-1301, miR-663, miR-339-5p, miR-149-star, miR-150, miR-18a, miR-103, miR-191, miR-296-3p, miR-31, miR-107*, miR-93*, miR-1275*, miR-181B*, miR-921*, miR-1225-5p, miR-1202, and miR-342-3p and (b) diagnosing the skin sample as containing melanoma when a difference in the level of the two or more miRNAs compared to a reference level indicates melanoma in the sample. 
     In specific embodiments, melanoma is diagnosed in the skin sample by alterations in the level of an miRNA compared to a reference level, with the following alterations observed in at least two miRNA selected from the group consisting of: miR-1268 decrease, miR-1228-star decrease, miR-92b-star decrease, miR-155 increase, miR-345 increase, miR-425 increase, miR-132 increase, miR-1207-5p decrease, miR-1301 increase, miR-663 decrease, miR-339-5p increase, miR-149-star decrease, miR-150 increase, miR-18a increase, miR-103 increase, miR-191 increase, miR-296-3p decrease, miR-31 increase, miR-107* increase, miR-93* increase, miR-1275* decrease, miR-181B* increase, miR-921* decrease, miR-1225-5p increase, miR-1202 decrease, and miR-342-3p increase. 
     The method of the invention may further include internal controls, such as measuring the level of an miRNA selected from miR-27b, miR-195, miR-199b-3p, and miR-199a-3p. 
     In yet further embodiments, the level of two or more miRNAs are used to distinguish melanoma from normal skin, and nevi from normal skin. Additional miRNA levels may be assayed for this purpose. 
     The level of miRNA in the sample can be determined by microarray and/or quantitative real-time PCR. The method of the invention may be performed on a fresh skin sample, on a fixed and/or paraffin-embedded sample. In one embodiment the skin sample is formalin-fixed and paraffin-embedded. 
     The method of the invention may further comprise other steps in the diagnosis of melanoma, and the differentiation between nevi and melanoma, including histopathological assessment, and clinical assessment. In related embodiments, the clinical and/or histopathological evaluations may be converted into a score that can be combined with a score derived from miRNA levels, resulting in a diagnostic score that reflects the likelihood of melanoma. 
     The method of the invention may further include a step of isolating nucleic acids from the sample. An additional step may include amplification of the nucleic acid. 
     In further embodiments, the invention comprise a kit. In one embodiment, a kit for differentially diagnosing between melanoma and nevus in a skin sample comprises primers for the amplification of at least two miRNA selected from the group consisting of: miR-150, miR-149-star, miR-1308; miR-191.; miR-1228-star; ENSG00000199411_s; miR-1268; miR-923; miR-23a; miR-132; miR-1207.5p; miR-342.3p; U38B; and miR-155. 
     In another embodiment, the kit comprises primers for the amplification of at least two miRNAs selected from the group consisting of: miR-1268, miR-8-star, miR-92b-star, miR-155, miR-345, miR-425, miR-132, miR-1207-5p, miR-1301, miR-663, miR-339-5p, miR-149-star, miR-150, miR-18a, miR-103, miR-191, miR-296-3p, miR-31, miR-107*, miR-93*, miR-1275*, miR-181B*, miR-921*, miR-1225-5p, miR-1202, and miR-342-3p. 
     The kit may also primers for amplification of controls, such as miR-27b, miR-195, miR-199b-3p, and miR-199a-3p. 
     The kit may also include suitable buffers, reagents for isolating nucleic acid, and instructions for use Kits may also include a microarray for measuring miRNA levels. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURE 
         FIGS. 1-10  show, respectively, the best 2-10 miRNA combinations for differentiating melanoma (MM) from normal skin (NS), and the relevant error rates and AUC, as determined by different statistical algorithms. 
         FIGS. 11-23  show, respectively, the best 2-14 miRNA combinations for differentiating melanoma (MM) from nevi (NV), and the relevant error rates and AUC as determined by different statistical algorithms. 
         FIG. 24  shows the log 2 signal for three miRNA: miR-150 (miR-150), miR-149-star (miR-149-star), and hsa-miR-1308 (miR-1308) across 137 samples. Samples 1-19 are normal skin (NS), 20-57 nevi (NV), 58-115 melanoma (MM) and 116-137 metastatic melanoma (Mets). As can be appreciated, miR-150, miR-149-star and miR-1308 distinguish normal skin and nevi from melanoma and metastatic. 
         FIG. 25  shows an example of error rate and AUC from 9 programs analyzing the ability of groups of miRNA analytes to differentiate between melanoma and nevi. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The present inventors have discovered that the levels of miRNAs in skin samples is a powerful tool to differentiate melanoma from non-tumorous nevi and, thereby, replace or supplement traditional clinical and histological methods of diagnosis. 
     DEFINITIONS 
     The present technology is described herein using several definitions, as set forth throughout the specification. As used herein, unless otherwise stated, the singular forms “a,” “an,” and “the” include plural reference. Thus, for example, a reference to “a nucleic acid” is a reference to one or more nucleic acids. 
     As used herein, “about” will be understood by persons of ordinary skill in the art and will vary to some extent depending upon the context in which it is used. If there are uses of the term which are not clear to persons of ordinary skill in the art, given the context in which it is used, the term “about” in reference to quantitative measurements or values will mean up to plus or minus 10% of the enumerated value. 
     The term “amplification” or “amplify” as used herein means one or more methods known in the art for copying a target nucleic acid, thereby increasing the number of copies of a selected nucleic acid sequence. Amplification may be exponential or linear. A target nucleic acid may be either DNA or RNA. The sequences amplified in this manner form an “amplicon.” While the exemplary methods described hereinafter relate to amplification using the polymerase chain reaction (“PCR”), numerous other methods are known in the art for amplification of nucleic acids (e.g., isothermal methods, rolling circle methods, etc.). The skilled artisan will understand that these other methods may be used either in place of, or together with, PCR methods. See, e.g., Saiki, “Amplification of Genomic DNA” in  PCR Protocols , Innis et al., Eds., Academic Press, San Diego, Calif. 1990, pp. 13-20; Wharam et al.,  Nucleic Acids Res.,  2001, 29(11):E54-E54; Hafner et al.,  Biotechniques  2001, 30(4):852-6, 858, 860; Zhong et al.,  Biotechniques,  2001, 30(4):852-6, 858, 860. 
     The terms “determining,” “measuring,” “assessing,” and “assaying” are used interchangeably and include both quantitative and qualitative measurement, and include determining if a characteristic, trait, or feature is present or not. Assessing may be relative or absolute. “Assessing the presence of” includes determining the amount of something present, as well as determining whether it is present or absent. 
     The term “clinical factors” as used herein, refers to any data that a medical practitioner may consider in determining a diagnosis of melanoma. Such factors include, but are not limited to, the patient&#39;s medical history, age, gender, skin color, a physical examination of the patient, and histopathology. 
     The term “complement” used herein means the complementary sequence to a nucleic acid according to standard Watson/Crick base pairing rules. A complement sequence can also be a sequence of RNA complementary to the DNA sequence or its complement sequence, and can also be a cDNA. The term “substantially complementary” as used herein means that two sequences hybridize under stringent hybridization conditions. The skilled artisan will understand that substantially complementary sequences need not hybridize along their entire length. In particular, substantially complementary sequences comprise a contiguous sequence of bases that do not hybridize to a target or marker sequence, positioned 3′ or 5′ to a contiguous sequence of bases that hybridize under stringent hybridization conditions to a target or marker sequence. 
     As used herein, the term “diagnosis” means detecting melanoma or the presence of melanoma cells. Usually, a diagnosis of a disease or disorder is based on the evaluation of one or more factors and/or symptoms that are indicative of the disease. That is, a diagnosis can be made based on the presence, absence or amount of a factor which is indicative of presence or absence of the disease or condition. Each factor or symptom that is considered to be indicative for the diagnosis of a particular disease does not need be exclusively related to the particular disease; i.e. there may be differential diagnoses that can be inferred from a diagnostic factor or symptom. Likewise, there may be instances where a factor or symptom that is indicative of a particular disease is present in an individual that does not have the particular disease. The term “diagnosis” also encompasses determining the therapeutic effect of a drug therapy, or predicting the pattern of response to a drug therapy. The diagnostic methods may be used independently, or in combination with other diagnosing and/or staging methods known in the medical art for a particular disease or disorder, particularly melanoma. 
     As used herein, the phrase “difference of the level” refers to differences in the quantity of a particular marker, such as a nucleic acid or a protein, in a sample as compared to a control or reference level. For example, the quantity of a particular biomarker may be present at an elevated amount or at a decreased amount in samples of patients with a neoplastic disease compared to a reference level. In one embodiment, a “difference of a level” may be a difference between the quantity of a particular biomarker present in a sample as compared to a control of at least about 1%, at least about 2%, at least about 3%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 50%, at least about 60%, at least about 75%, at least about 80% or more. In one embodiment, a “difference of a level” may be a statistically significant difference between the quantity of a biomarker present in a sample as compared to a control. For example, a difference may be statistically significant if the measured level of the biomarker falls outside of about 1.0 standard deviations, about 1.5 standard deviations, about 2.0 standard deviations, or about 2.5 stand deviations of the mean of any control or reference group. 
     By “isolated”, when referring to a nucleic acid (e.g., an oligonucleotide such as RNA, DNA, or a mixed polymer) is meant a nucleic acid that is apart from a substantial portion of the genome in which it naturally occurs and/or is substantially separated from other cellular components which naturally accompany such nucleic acid. For example, any nucleic acid that has been produced synthetically (e.g., by serial base condensation) is considered to be isolated. Likewise, nucleic acids that are recombinantly expressed, cloned, produced by a primer extension reaction (e.g., PCR), or otherwise excised from a genome are also considered to be isolated. In some embodiments, the nucleic acid is isolated from the skin sample before further processing, such as PCR. 
     The term “label” as used herein, refers to any physical molecule directly or indirectly associated with a specific binding agent or antigen which provides a means for detection for that antibody or antigen. A “detectable label” as used herein refers any moiety used to achieve signal to measure the amount of complex formation between a target and a binding agent. These labels are detectable by spectroscopic, photochemical, biochemical, immunochemical, electromagnetic, radiochemical, or chemical means, such as fluorescence, chemifluoresence, or chemiluminescence, electrochemiluminescence or any other appropriate means. Suitable detectable labels include fluorescent dye molecules or fluorophores. 
     As used herein interchangeably, a “microRNA,” “miR,” or “miRNA” refers to the unprocessed or processed RNA transcript from a miRNA gene. MicroRNAs (miRNAs) are non-coding RNAs of 19-25 nucleotides in length that regulate gene expression by inducing translational inhibition or cleavage of their target mRNA through base pairing to partially or fully complementary sites. The unprocessed miRNA gene transcript is also called a “miRNA precursor,” and typically comprises an RNA transcript of about 70-100 nucleotides in length. The miRNA precursor can be processed by digestion with an RNAse (for example, Dicer, Argonaut, or RNAse III) into an active 19-25 nucleotide RNA molecule. This active 19-25 nucleotide RNA molecule is also called the “processed” miRNA gene transcript or “mature” miRNA. 
     As used herein, “nucleic acid” refers broadly to segments of a chromosome, segments or portions of DNA, cDNA, and/or RNA. Nucleic acid may be derived or obtained from an originally isolated nucleic acid sample from any source (e.g., isolated from, purified from, amplified from, cloned from, or reverse transcribed from sample DNA or RNA). 
     As used herein, the term “oligonucleotide” refers to a short polymer composed of deoxyribonucleotides, ribonucleotides or any combination thereof. Oligonucleotides are generally between about 10 and about 100 nucleotides in length. Oligonucleotides are typically 15 to 70 nucleotides long, with 20 to 26 nucleotides being the most common. An oligonucleotide may be used as a primer or as a probe. An oligonucleotide is “specific” for a nucleic acid if the oligonucleotide has at least 50% sequence identity with a portion of the nucleic acid when the oligonucleotide and the nucleic acid are aligned. An oligonucleotide that is specific for a nucleic acid is one that, under the appropriate hybridization or washing conditions, is capable of hybridizing to the target of interest and not substantially hybridizing to nucleic acids which are not of interest. Higher levels of sequence identity are preferred and include at least 75%, at least 80%, at least 85%, at least 90%, or at least 95% sequence identity. 
     As used herein, a “primer” for amplification is an oligonucleotide that specifically anneals to a target or marker nucleotide sequence. The 3′ nucleotide of the primer should be identical to the target or marker sequence at a corresponding nucleotide position for optimal primer extension by a polymerase. As used herein, a “forward primer” is a primer that anneals to the anti-sense strand of double stranded DNA (dsDNA). A “reverse primer” anneals to the sense-strand of dsDNA. 
     As used herein, the term “reference level” refers to a level of a substance which may be of interest for comparative purposes. In one embodiment, a reference level may be the miRNA levels expressed as an average of the level of miRNA from an area of normal skin or skin containing nevi and not melanoma. Nucleic acid samples may also be normalized relative to an internal control nucleic acid. 
     As used herein, the term “sample” refers to a skin biopsy from the subject, such as would typically be used for histopathological examination, or any section derived from such a sample. That is, a suspected melanoma may be entirely excised from the skin, but the biopsy is fixed and embedded in paraffin, and sectioned for further examination. 
     As used herein, the term “subject” refers to a mammal, such as a human, but can also be another animal such as a domestic animal (e.g., a dog, cat, or the like), a farm animal (e.g., a cow, a sheep, a pig, a horse, or the like) or a laboratory animal (e.g., a monkey, a rat, a mouse, a rabbit, a guinea pig, or the like). The term “patient” refers to a subject who is, or is suspected to be, afflicted with melanoma. 
     The phrase “substantially the same as” in reference to a comparison of one value to another value for the purposes of clinical management of a disease or disorder means that the values are statistically not different. Differences between the values can vary, for example, one value may be within 20%, within 10%, or within 5% of the other value. 
     As used herein, the term “diagnostic score” refers to a single number or score, based on a statistical analysis of the measured level of one or more biomarkers that reflects a relationship of a specific subject to any one particular group of individuals, such as normal individuals or individuals having a disease or any progressive state thereof. In some embodiments, the diagnostic score is derived from a quantitative multivariate analysis, which reflects the overall statistical assessment of an individual patient&#39;s clinical condition based upon an integrated statistical calculation of a plurality of qualitatively unique factors, e.g., levels of diagnostic miRNA, combined with clinical presentation, etc. 
     Melanoma and Nevi 
     Nevus (or naevus, plural nevi or naevi, from nævus, Latin for “birthmark”) is the medical term for sharply-circumscribed and chronic lesions of the skin. These lesions are commonly named birthmarks and moles. Nevi are benign by definition. 
     A melanocytic nevus (nevomelanocytic nevus, nevocellular nevus) is a benign proliferation of melanocytes, and are very common; almost all adults have at least one, usually more. A melanocytic nevus may be congenital or acquired. 
     A dysplastic nevus usually an acquired melanocytic nevus with abnormal features making it difficult to distinguish from a melanoma. It can be a marker for an individual at risk for developing melanomas. 
     Melanoma is a malignant tumor of melanocytes. Melanocytes predominantly occur in skin, between the outer layer of the skin (the epidermis) and the next layer (the dermis), but are also found in other parts of the body, including the bowel and the eye (see uveal melanoma). Melanoma can occur in any part of the body that contains melanocytes. Melanoma is less common than other skin cancers but is much more dangerous and causes the majority (75%) of deaths related to skin cancer. 
     Melanoma arises from DNA damage to melanocytcs. The early stage of the disease is called the radial growth phase, and the tumour is less than 1 mm thick. Next is the invasive radial growth phase, when individual cells start to acquire invasive potential. The Breslow&#39;s depth of the lesion is usually less than 1 mm (0.04 in), the Clark level is usually 2. The following step is invasive melanoma, “vertical growth phase” (VGP). The tumour attains invasive potential, growing into the surrounding tissue and can spread around the body through blood or lymph vessels to form metastases. The tumour thickness is usually more than 1 mm (0.04 in), and the tumour involves the deeper parts of the dermis. 
     An immunological reaction against the tumour during the VGP may be judged by the presence and activity of the tumour infiltrating lymphocytes (TILs). These cells sometimes completely destroy the primary tumour, this is called regression, which is the latest stage of the melanoma development. In certain cases, the primary tumour is completely destroyed and only the metastatic tumour is discovered. 
     Melanoma may also have a genetic predisposition. Mutations in CDKN2A, CDK4, MC1R, MDM2 SNP309 and those associated with xeroderma pigmentosum (XP) predispose one to melanoma. Familial melanoma is genetically heterogeneous,[10] and loci for familial melanoma have been identified on the chromosome arms 1p, 9p and 12q. Multiple genetic events have been related to the pathogenesis (disease development) of melanoma. 
     Clinical and Pathological Diagnosis 
     Melanoma is usually first detected by visual examination of the skin, notably (A) asymmetry, (B) a border that is uneven, ragged, or notched, (C) coloring of different shades of brown, black, or tan and (D) diameter that had changed in size. Normal moles are symmetrical, have an even border, even color, and no change in diameter. The main concern is distinguishing between a benign nevus, a dysplastic nevus, and a melanoma. Moles that are irregular in color or shape are often treated as candidates of melanoma. Following a visual examination and a dermatoseopic exam, or in vivo diagnostic tools such as a confocal microscope, a sample (biopsy) of the suspicious mole may be obtained. 
     Sample Preparation 
     When an atypical mole has been identified, a skin biopsy takes place in order to best diagnose it. Local anesthetic is used to numb the area, then the mole is biopsied. The biopsy material is then sent to a laboratory to be evaluated by a pathologist. A skin biopsy can be a punch, shave, or complete excision. The complete excision is the preferred method, but a punch biopsy can suffice if the patient has cosmetic concerns (i.e. the patient does not want a scar) and the lesion is small. A scoop or deep shave biopsy is often advocated, but should be avoided due to risk of a recurrent nevus, which can complicate future diagnosis of a melanoma, and the possibility that resulting scar tissue can obscure tumor depth if a melanoma is found to be present and re-excised. 
     Most dermatologists and dermatopathologists use a system devised by the NIH for classifying melanocytic lesions. In this classification, a nevus can be defined as benign, having atypia, or being a melanoma. A benign nevus is read as (or understood as) having no cytologic or architectural atypia. An atypical mole is read as having architectural atypia, and having (mild, moderate, or severe) cytologic (melanocytic) atypia. Usually, cytologic atypia is of more important clinical concern than architectural atypia. Usually, moderate to severe cytologic atypia will require further excision to make sure that the surgical margin is completely clear of the lesion. 
     The most important aspect of the biopsy report is that the pathologist indicates if the margin is clear (negative or free of melanocytic nevus), or if further tissue (a second surgery) is required. If this is not mentioned, usually a dermatologist or clinician will require further surgery if moderate to severe cytologic atypia is present—and if residual nevus is present at the surgical margin. 
     miRNA Markers to Distinguish Nevi from Melanoma 
     Distinguishing nevi from melanoma requires a high degree of skill. Misdiagnosis of a melanoma as a nevus can result in delay in treatment, which can be lethal because melanoma is an aggressive cancer that requires prompt intervention. Conversely, incorrectly identifying a nevus as a melanoma may subject a patient to aggressive treatment that is unnecessary and harmful. The present inventors have established that melanoma may be distinguished from nevi by monitoring the levels of select miRNA. 
     The methods described herein can distinguish melanoma, normal skin, nevi and malignant melanoma. Most importantly, the method is suitable for differentiating nevi from melanoma, and therefore fill a need for diagnosis that it not fully met by histology. As such, the methods of the invention can replace, supplement, or confirm histology. A particular advantage of the methods of the invention is that they provide independent objective evidence. 
     An additional advantage of the invention is that they can be performed on formalin fixed paraffin embedded (FFPE) tissue, and therefore can be used on the same samples that are processed for standard histopathological examination, and thus do not require a separate sample, or special handling. Another advantage is that the inventors have found that the miRNAs are stable in FFPE tissue and can be detected some time after fixing and embedding. 
     Another advantage of the invention is that the mRNA&#39;s chosen do not require a relatively pure sample of melanoma cells, and can detect melanoma in a sample that also contains normal skin, nevi and other skin cells. Thus, the miRNA assay is not overly sensitive to contamination nor require special handling beyond that which is normally used for preparation of FFPE tissue for regular histology. 
     The inventors have identified 50 miRNAs that can readily distinguish melanoma from nevi. Through statistical modeling and analysis, groups of the best 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 and 14 markers have been identified. An increase in the number of markers used may improve sensitivity and accuracy, but comes with increased cost and complexity. 
     Additional diagnostic markers may be combined with the miRNA measurements to further aid diagnosis. For example, the clinical and/or histopathological results can be converted into a score, which is then combined with a score derived from the miRNA data. The combination of scores can be used to obtain a single “diagnostic score” that reflects the likelihood of melanoma. 
     Nucleic Acid Extraction and Detection 
     The level of a miRNA gene product in a sample can be measured using any technique that is suitable for detecting RNA expression levels in a biological sample. Suitable techniques for determining RNA expression levels in a biological sample are well known to those of skill in the art. These include, for example, Northern blot analysis, RT-PCR, and in situ hybridization. 
     The nucleic acid to be detected may be from a biological sample such as a tissue sample and the like. Various methods of extraction are suitable for isolating the DNA or RNA. Suitable methods include phenol and chloroform extraction. See Maniatis et al.,  Molecular Cloning, A Laboratory Manual,  2d, Cold Spring Harbor Laboratory Press, pp. 16-54 (1989). Numerous commercial kits also yield suitable DNA and RNA including, but not limited to, QIAamp™ mini blood kit, Agencourt Genfind™, Roche Cobas® Roche MagNA Pure® or phenol:chloroform extraction using Eppendorf Phase Lock Gels®, and the NucliSens extraction kit (Biomerieux, Marcy l&#39;Etoile, France). In an illustrative embodiment, RNA is isolated from patient serum on the NucliSens easyMAG system (Biomeriux SA, France) according to the manufacturer&#39;s protocol. 
     In one embodiment, the level of at least one miRNA gene product is detected using Northern blot analysis. For example, total RNA can be purified from a sample in the presence of nucleic acid extraction buffer, followed by centrifugation. Nucleic acids are precipitated, and DNA is removed by treatment with DNase and precipitation. The RNA molecules are then separated by gel electrophoresis on agarose gels according to standard techniques, and transferred to nitrocellulose filters. The RNA is then immobilized on the filters by heating. Detection and quantification of specific RNA is accomplished using appropriately labeled DNA or RNA probes complementary to the RNA in question. See, for example,  Molecular Cloning: A Laboratory Manual , J. Sambrook et al., eds., 2nd edition, Cold Spring Harbor Laboratory Press, 1989, Chapter 7. 
     Suitable probes (e.g., DNA probes or RNA probes) for Northern blot hybridization of a given miRNA gene product can be produced from the known nucleic acid sequences and include, but are not limited to, probes having at least about 70%, 75%, 80%, 85%, 90%, 95%, 98% or 99% complementarity to a miRNA gene product of interest, as well as probes that have complete complementarity to a miRNA gene product of interest. Methods for preparation of labeled DNA and RNA probes, and the conditions for hybridization thereof to target nucleotide sequences, are described in  Molecular Cloning: A Laboratory Manual , J. Sambrook et al., eds., 2nd edition. Cold Spring Harbor Laboratory Press, 1989, Chapters 10 and 11. 
     For example, the nucleic acid probe can be labeled with, e.g., a radionuclide, such as  3 H,  32 P,  33 P  14 C, or  35 S; a heavy metal; a ligand capable of functioning as a specific binding pair member for a labeled ligand (e.g., biotin, avidin or an antibody); a fluorescent molecule; a chemiluminescent molecule; an enzyme or the like. Probes can be labeled to high specific activity by either the nick translation method or by the random priming method. Autoradiographic detection of hybridization can then be performed by exposing hybridized filters to photographic film. Densitometric scanning of the photographic films exposed by the hybridized filters provides an accurate measurement of miRNA levels. Using another approach, miRNA gene transcript levels can be quantified by computerized imaging systems. 
     In one embodiment, the miRNA is detected using a nucleic acid amplification process. Nucleic acid extracted from a sample can be amplified using nucleic acid amplification techniques well known in the art. By way of example, but not by way of limitation, these techniques can include the polymerase chain reaction (PCR), reverse transcriptase polymerase chain reaction (RT-PCR), nested PCR, ligase chain reaction. See Abravaya, K., et al.,  Nucleic Acids Research,  23:675-682, (1995), branched DNA signal amplification, Urdea, M. S., et al.,  AIDS,  7 (suppl 2):S11-S14, (1993), amplifiable RNA reporters, Q-beta replication, transcription-based amplification, boomerang DNA amplification, strand displacement activation, cycling probe technology, isothermal nucleic acid sequence based amplification (NASBA). See Kievits, T. et al.,  J Virological Methods,  35:273-286, (1991), Invader Technology, or other sequence replication assays or signal amplification assays may also be used. 
     Some methods employ reverse transcription of RNA to cDNA. The method of reverse transcription and amplification may be performed by previously published or recommended procedures. Various reverse transcriptases may be used, including, but not limited to, MMLV RT, RNase H mutants of MMLV RT such as Superscript and Superscript II (Life Technologies, GIBCO BRL, Gaithersburg, Md.), AMV RT, and thermostable reverse transcriptase from  Thermus thermophilus . For example, one method which may be used to convert RNA to cDNA is the protocol adapted from the Superscript II Preamplification system (Life Technologies, GIBCO BRL, Gaithersburg, Md.; catalog no. 18089-011), as described by Rashtchian, A.,  PCR Methods Applic.,  4:S83-S91, (1994). 
     In a suitable embodiment, PCR is used to amplify a target sequence of interest, PCR is a technique for making many copies of a specific template DNA sequence. The reaction consists of multiple amplification cycles and is initiated using a pair of primer sequences that hybridize to the 5′ and 3′ ends of the sequence to be copied. The amplification cycle includes an initial denaturation, and typically up to 50 cycles of annealing, strand elongation and strand separation (denaturation). In each cycle of the reaction, the DNA sequence between the primers is copied. Printers can bind to the copied DNA as well as the original template sequence, so the total number of copies increases exponentially with time. PCR can be performed as according to Whelan et al.,  J of Clin Micro,  33(3):556-561 (1995). Briefly, a PCR reaction mixture includes two specific primers, dNTPs, approximately 0.25 U of Taq polymerase, and 1×PCR Buffer. 
     The skilled artisan is capable of designing and preparing primers that are appropriate for amplifying a target or marker sequence. The length of the amplification primers depends on several factors including the nucleotide sequence identity and the temperature at which these nucleic acids are hybridized or used during in vitro nucleic acid amplification. The considerations necessary to determine a preferred length for an amplification primer of a particular sequence identity are well-known to a person of ordinary skill. For example, the length of a short nucleic acid or oligonucleotide can relate to its hybridization specificity or selectivity. 
     In some embodiments, the amplification may include a labeled primer or probe, thereby allowing detection of the amplification products corresponding to that primer or probe. In particular embodiments, the amplification may include a multiplicity of labeled primers or probes; such primers may be distinguishably labeled, allowing the simultaneous detection of multiple amplification products. Oligonucleotide probes can be designed which are between about 10 and about 100 nucleotides in length and hybridize to the amplified region. Oligonucleotides probes are preferably 12 to 70 nucleotides; more preferably 15-60 nucleotides in length; and most preferably 15-25 nucleotides in length. The probe may be labeled. 
     In one embodiment, a primer or probe is labeled with a fluorogenic reporter dye that emits a detectable signal. While a suitable reporter dye is a fluorescent dye, any reporter dye that can be attached to a detection reagent such as an oligonucleotide probe or primer is suitable for use in the invention. Such dyes include, but are not limited to, Acridine, AMCA, BODIPY, Cascade Blue, Cy2, Cy3, Cy5, Cy7, Edans, Eosin, Erythrosin, Fluorescein, 6-Fam, Tet, Joe, Hex, Oregon Green, Rhodamine, Rhodol Green, Tamra, Roz, and Texas Red. 
     In yet another embodiment, the detection reagent may be further labeled with a quencher dye such as Tamra, Dabcyl, or Black Hole Quencher® (BHQ), especially when the reagent is used as a self-quenching probe such as a TaqMan® (U.S. Pat. Nos. 5,210,015 and 5,538,848) or Molecular Beacon probe (U.S. Pat. Nos. 5,118,801 and 5,312,728), or other stemless or linear beacon probe (Livak et al., 1995,  PCR Method Appl.,  4:357-362; Tyagi et al, 1996,  Nature Biotechnology,  14:303-308; Nazarenko et al., 1997,  Nucl. Acids Res.,  25:2516-2521; U.S. Pat. Nos. 5,866,336 and 6,117,635). 
     Nucleic acids may be amplified prior to detection or may be detected directly during an amplification step (i.e., “real-time” methods). For example, amplified fragments may be detected using standard gel electrophoresis methods. In some embodiments, amplified fractions are separated on an agarose gel and stained with ethidium bromide by methods known in the art to detect amplified fragments. In some embodiments, the target sequence is amplified using a labeled primer such that the resulting amplicon is detectably labeled. In some embodiments, the primer is fluorescently labeled. 
     In one embodiment, detection of a miRNA, such as a nucleic acid from an a miR-16 or miR-199a, is performed using the TaqMan® assay, which is also known as the 5′ nuclease assay (U.S. Pat. Nos. 5,210,015 and 5,538,848). The TaqMan® assay detects the accumulation of a specific amplified product during PCR. The TaqMan® assay utilizes an oligonucleotide probe labeled with a fluorescent reporter dye and a quencher dye. The reporter dye is excited by irradiation at an appropriate wavelength, it transfers energy to the quencher dye in the same probe via a process called fluorescence resonance energy transfer (FRET). When attached to the probe, the excited reporter dye does not emit a signal. The proximity of the quencher dye to the reporter dye in the intact probe maintains a reduced fluorescence for the reporter. The reporter dye and quencher dye may be at the 5′ most and the 3′ most ends, respectively or vice versa. Alternatively, the reporter dye may be at the 5′ or 3′ most end while the quencher dye is attached to an internal nucleotide, or vice versa. In yet another embodiment, both the reporter and the quencher may be attached to internal nucleotides at a distance from each other such that fluorescence of the reporter is reduced. 
     During PCR, the 5′ nuclease activity of DNA polymerase cleaves the probe, thereby separating the reporter dye and the quencher dye and resulting in increased fluorescence of the reporter. Accumulation of PCR product is detected directly by monitoring the increase in fluorescence of the reporter dye. The DNA polymerase cleaves the probe between the reporter dye and the quencher dye only if the probe hybridizes to the target-containing template which is amplified during PCR. 
     TaqMan® primer and probe sequences can readily be determined using the nucleic acid sequence information of the miRNA of interest. A number of computer programs, such as Primer Express (Applied Biosystems, Foster City, Calif.), can be used to rapidly obtain optimal primer/probe sets. It will be apparent to one of skill in the art that such primers and probes for detecting the target nucleic acids are useful in diagnostic assays for neoplastic disorders, such as HCC, and can be readily incorporated into a kit format. The present invention also includes modifications of the TaqMan® assay well known in the art such as the use of Molecular Beacon probes (U.S. Pat. Nos. 5,118,801 and 5,312,728) and other variant formats (U.S. Pat. Nos. 5,866,336 and 6,117,635). 
     In an illustrative embodiment, real time PCR is performed using TaqMan® Assays in combination with a suitable amplification/analyzer such as the ABI Prism® 7900HT Sequence Detection System. The ABI PRISM® 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Real-time detection on the ABI Prism 7900HT or 7900HT Sequence Detector monitors fluorescence and calculates Rn during each PCR cycle. The threshold cycle, or Ct value, is the cycle at which fluorescence intersects the threshold value. The threshold value is determined by the sequence detection system software or manually. The Ct can be correlated to the initial amount of nucleic acids or number of starting cells using a standard curve. 
     In one embodiment, TaqMan® MicroRNA Assays are used to detect the miRNA. TaqMan® MicroRNA Assays are predesigned assays that are available for the majority of content found on the miRBase miRNA sequence repository. In another embodiment, the mirVana™ qRT-PCR miRNA Detection Kit (Ambion) is a used to detect and quantify the miRNA. This is a quantitative reverse transcription-PCR (qRT-PCR) kit enabling sensitive, rapid quantification of miRNA (miRNA) expression from total RNA samples. 
     As a quality control measure, an internal amplification control may be included in one or more samples to be extracted and amplified. The skilled artisan will understand that any detectable sequence that is not typically present in the sample can be used as the control sequence. A control sequence can be produced synthetically. If PCR amplification is successful, the internal amplification control amplicons can then be detected. Additionally, if included in the sample prior to purification of nucleic acids, the control sequences can also act as a positive purification control. 
     Statistical Methods 
     Statistical methods can be used to set thresholds for determining when the level in a subject can be considered to be different than or similar to a reference level. In addition, statistics can be used to determine the validity of the difference or similarity observed between a patient&#39;s circulating miRNA level and the reference level. Useful statistical analysis methods are described in L. D. Fisher &amp; G. vanBelle,  Biostatistics: A Methodology for the Health Sciences  (Wiley-Interscience, NY, 1993). For instance, confidence (“p”) values can be calculated using an unpaired 2-tailed t test, with a difference between groups deemed significant if the p value is less than or equal to 0.05. As used herein a “confidence interval” or “CI” refers to a measure of the precision of an estimated or calculated value. The interval represents the range of values, consistent with the data that is believed to encompass the “true” value with high probability (usually 95%). The confidence interval is expressed in the same units as the estimate or calculated value. Wider intervals indicate lower precision; narrow intervals indicate greater precision. Preferred confidence intervals of the invention are 90%, 95%, 97.5%, 98%, 99%, 99.5%, 99.9% and 99.99%. A “p-value” as used herein refers to a measure of probability that a difference between groups happened by chance. For example, a difference between two groups having a p-value of 0.01 (or p=0.01) means that there is a 1 in 100 chance the result occurred by chance. Preferred p values are 0.1, 0.05, 0.025, 0.02, 0.01, 0.005, 0.001, and 0.0001. Confidence intervals and p-values can be determined by methods well-known in the art. See, e.g., Dowdy and Wearden,  Statistics for Research , John Wiley &amp; Sons, New York, 1983. 
     On linear model for assessing differential expression in microarray experiments: Smith G K (2004) “Linear models and empirical bayes method for assessing differential expression in microarray experiments”  Statistical Applications in Genetics and Molecular Biology . For AUC calculation: Mason S J and Graham N E (1982) “Areas beneath the relative operating characteristics (ROC) and relative operating levels (ROL) curves: Statistical significance and interpretation,”  Q. J. R. Meteorol. Soc . textbf30 291-303. Multiple algorithms program for marker combination selection: An R based program with nine algorithms including random forest, ada boosting, svm, bagging, logistic regression, lasso, matt, cart, ctree is available, for example, as open-source software from the R Foundation. Random forests were also conducted according to Breiman, L. (2001),  Random Forests , Machine Learning 45(1), 5-32. Sec also Breiman, L (2002), “Manual On Setting Up, Using, And Understanding Random Forests V3.1. 
     In connection with miRNA used to diagnose melanoma, one may seek levels that are lower or higher than a control. The term “elevated levels” or “higher levels” as used herein refers to levels of an miRNA that are higher than what would normally be observed in a comparable sample from control or normal subjects or normal tissue from the patient (i.e., a reference value). Similarly, “reduced levels” or “lower levels” as used herein refer to levels of that are lower than what would normally be observed in a comparable sample from control or normal subjects, or normal tissue from the patient (i.e., a reference value). In some embodiments, “control levels” (i.e., normal levels) refer to a range of miRNA levels that would be normally be expected to be observed in nevi, or normal skin. A control level may be used as a reference level for comparative purposes. The ranges accepted as outside “control levels” are dependent on a number of factors. For example, one laboratory may routinely determine the level of circulating miRNA in a sample that is different than the miRNA obtained for the same sample by another laboratory. Also, different assay methods may achieve different value ranges. Value ranges may also differ in various sample types, for example, different body fluids or by different treatments of the sample. One of ordinary skill in the art is capable of considering the relevant factors and establishing appropriate reference ranges for “control values” and “elevated/reduced values” of the present invention. For example, a series of samples from control subjects and subjects diagnosed with melanoma can be used to establish ranges that are “normal” or “control” levels and ranges that are “elevated” or “reduced” than the control range. 
     The level of one or more miRNAs measured in the test sample is normalized, such as by comparison to an internal reference nucleic acid, e.g., U44 or small RNA U6. The levels of the one or more miRNAs may then be compared to a reference value to determine if the levels of the one or more miRNAs are elevated or reduced relative to the reference value. Typically, the reference value is the level measured in a comparable sample from one or more healthy individuals. An increase or decrease in the level of the one or more miRNAs may be used in conjunction with clinical factors to diagnose melanoma. 
     In some embodiments, the level of one or more miRNAs is combined with one or more additional markers to improve diagnostic sensitivity and specificity. Exemplary markers include, but are not limited to, any useful diagnostic marker associated with melanoma including those which may be assessed by fluorescence in-situ hybridization (FISH) and/or comparative genomic hybridization (CGH). 
     Kits 
     A kit may be used for conducting the diagnostic and prognostic methods described herein. Typically, the kit should contain, in a carrier or compartmentalized container, reagents useful in any of the above-described embodiments of the diagnostic method. The carrier can be a container or support, in the form of e.g., bag, box, tube, rack, and is optionally compartmentalized. The carrier may define an enclosed confinement for safety purposes during shipment and storage. In one embodiment, the kit includes one or more PCR primers capable of amplifying miRNA selected from miR-132, miR-150, miR-339-5p, miR-15b, miR-342-3p, miR-572, miR-155, miR-425, miR-1202, miR-1268, HBII-382_s, miR-1225-5p, miR-30c, miR-106b-star, miR-125a-5p, mgU6-53B, miR-25, miR-149-star, miR-939, miR-92b-star, miR-500-star, miR-22, HBII-142_x, miR-181b, HBII-142, U38B, miR-663, miR-1224-5p, miR-23a, HBII-85-6_x, miR-1207-5p, miR-1301, miR-1228-star, miR-345, miR-30a-star, ENSG00000199411_s, ENSG00000202327, miR-92a, miR-127-3p, HBII-85-26, miR-1308, miR-31, miR-921, miR-146b-5p, miR-768-3p, miR-708, miR-139-5p, ACA24_x, miR-501-3p, miR-502-3p, miR-923, and miR-191. 
     In further embodiments, the invention comprise a kit. In one embodiment, a kit for differentially diagnosing between melanoma and nevus in a skin sample comprises primers for the amplification of at least two miRNA selected from the group consisting of miR-150, miR-149-star, miR-1308; miR-191; miR-1228-star; ENSG00000199411_s; miR-1268; miR-923; miR-23a: miR-132; miR-1207.5p; miR-342.3p; U38B; and miR-155. 
     In another embodiment, the kit comprises primers for the amplification of at least two miRNAs selected from the group consisting of: miR-1268, miR-1228-star, miR-92b-star, miR-155, miR-345, miR-425, miR-132, miR-1207-5p, miR-1301, miR-663, miR-339-5p, miR-149-star, miR-150, miR-18a, miR-103, miR-191, miR-296-3p, miR-31, miR-107*, miR-93*, miR-1275*, miR-181B*, miR-921*, miR-1225-5p, miR-1202, and miR-342-3p. 
     The kit may also primers for amplification of controls, such as miR-27b, miR-195, miR-199b-3p, and miR-199a-3p. 
     The kit may also include suitable buffers, reagents for isolating nucleic acid, and instructions for use. Kits may also include a microarray for measuring miRNA level. 
     The primers may be labeled with a detectable marker such as radioactive isotopes, or fluorescence markers. Instructions for using the kit or reagents contained therein are also included in the kit. 
     EXAMPLES 
     The present methods and kits, thus generally described, will be understood more readily by reference to the following examples, which are provided by way of illustration and are not intended to be limiting of the present methods and kits. The following is a description of the materials and experimental procedures used in the example. 
     Example 1 
     137 samples were examined: (1) 19 normal skin; (2) 38 nevi, of which 20 were intradermal, where the nevus cells are located in the dermis only, and 18 were compound nevus, which is a mixture of junctional and intradermal proliferation and are slightly raised and brown to black; (3) 58 primary melanoma; (4) 22 metastatic melanoma. 
     Total RNA, including low molecular weight RNA, was isolated from ten 10 μm-FFPE sections with RecoverAll RNA extraction kit (Applied Biosystems), according to the manufacturer&#39;s protocol. 
     The Affymetrix GeneChip® miRNA array was used, according to the manufacturer&#39;s protocol, to evaluate miRNA expression in FFPE samples. The miRNA array covers 71 organisms, including human, mouse, rat, and monkey, and contains 1801 sets of human miRNA, snoRNAs and scaRNAs. Samples were labeled using the Genisphere FlashTag™ Biotin Labeling Assay, which utilizes the 3DNA™ technology. The 3DNA™ dendrimer was ligated to samples to allow multiple biotin molecules (˜15) to bind to each poly-A tailed RNA molecule. Following FlashTag™ ligation, samples were hybridized on the Affymetrix GeneChip® miRNA array overnight. The hybridized chips were washed and processed to scan in an Affimatrix GeneChip Scanner 3000 7G. 
     Statistical Analyses 
     The raw microarray data was analyzed using the miRNA QC tool which performed an RNA normalization and extracted signals for data analysis. Log 2 values were used for miRNA expression in each group. The log 2 fold change (log 2 FC) was calculated by subtracting the mean of log 2 of group 1 from the mean log 2 of group 2. Student&#39;s t test was used to compare the miRNA expression level of each miRNA between normal skin, nevi, melanoma, and metastatic melanoma. P&lt;0.05 was used as statistical significance. The area under ROC (AUC) was calculated to reflect the separation between each group. The default AUC value is 0.5 meaning no separation, and the maximum possible value is 1.0, meaning complete separation between each group. 
     A screen on probe signal detection in samples was performed having of 137 samples at least &gt;30 samples with signal detectable. There are 729 of 1801 human probes satisfying such condition. These 729 candidate markers were analyzed using multiple algorithm programs. 
     Two or three markers completely separate these melanoma from nevi, as confirmed by multiple algorithms. The best 15 miRNA markers from randomForest share 14 markers overlap with the best 15 markers from boosting. In addition, these 14 best overlap markers include 6 of 7 best markers from previous analyses of 40 melanoma vs 20 nevi. These results therefore show consistency across different assays at different times. 
     Example 2 
     Additional experiments were performed to identify miRNA that can distinguish between different skin conditions. 
     For melanoma vs nevus, specimens (n=380) included a training set of 20 paraffin-embedded blocks of normal skin, 60 paraffin-embedded blocks of skin biopsies with benign nevus, 60 paraffin-embedded blocks of skin biopsies with malignant melanoma. Next, a validation set of 100 paraffin-embedded blocks of skin biopsies with benign nevus, 100 paraffin-embedded blocks of skin biopsies with malignant melanoma and 50 paraffin-embedded blocks of dysplastic nevus. 
     To distinguish between primary melanoma and metastatic melanoma, 180 study specimens included a training set of 60 paraffin-embedded blocks of skin biopsies with malignant melanoma and 30 paraffin-embedded blocks of metastatic melanoma. The validation set comprised 60 paraffin-embedded blocks of skin biopsies with malignant melanoma and 30 paraffin-embedded blocks of metastatic melanoma. 
     The subject population targeted all ethnicities and was approximately 50% male and 50% female. Specimens of adults 18 year and younger, and all subjects 89 years or older (“&gt;90”) were discarded. 
     Tissues (normal benign, nevi and indeterminate nevus, malignant melanoma and metastatic melanoma) were collected at DermaPath. 240 nevi and 40 melanoma FFPEs were purchased from BioTheme. 
     Total RNA, including low molecular weight RNA, was isolated from ten 10 μm-FFPE sections with RecoverAll RNA extraction kit from Applied Biosystems according to the manufacturer&#39;s protocol. 
     The Affymetrix GeneChip® miRNA array was used to evaluate miRNA expression in FFPE samples. The miRNA array covers 71 organisms, including human, mouse, rat, and monkey, and contains 1801 sets of human miRNA, snoRNAs and scaRNAs. 
     The experiment procedure was according to the Affymetrix miRNA expression analysis manual. Samples were labeled with the Genisphere FlashTag™ Biotin Labeling Assay, which utilizes the 3DNA™ technology. The 3DNA™ dendrimer was ligated to samples to allow multiple biotin molecules (˜15) to bind to each poly-A tailed RNA molecule. Following FlashTag™ ligation, samples were hybridized on the Affymetrix GeneChip® miRNA array overnight. The hybridized chips were washed and processed to scan in an Affimatrix GeneChip Scanner 3000 7G. 
     Statistical Analyses 
     Log 2 values were used for miRNA expression in each group. The log 2 fold change (log 2 FC) was calculated by subtracting the mean of log 2 of group 1 from the mean log 2 of group 2. Student&#39;s t test was used to compare the miRNA expression level of each miRNA between normal skin, nevi, melanoma, and metastatic melanoma. P&lt;0.05 was used as statistical significance. The area under ROC (AUC) was calculated to reflect the separation between each group. The default AUC value is 0.5 meaning no separation, and the maximum possible value is 1.0, meaning complete separation between each group. 
     Semi-Quantitative Reverse-Transcriptase PCR Analysis of miRNA 
     Two-step TaqMan reverse-transcriptase PCR analysis was performed for analysis of miRNAs. Reverse transcription was performed in a 15-μl reaction volume using specific primers for each miRNA contained in the TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, Foster City, Calif.) by sequentially incubating at 16° C. for 30 min, 42° C. for 30 min, and 85° C. for 5 min. Real-time PCR was done using the standard TaqMan MicroRNA assay protocol on an Applied Biosysytems 7900 system (Applied Biosystems). Each PCR mixture (20 μl) included the reverse transcription products, TaqMan 2× Universal PCR Master Mix without UNG Amperase, miRNA-specific TaqMan probes, and primers supplied by Applied Biosystems. The reactions were incubated in a 96-well plate with an initial denaturation at 95° C. for 10 min, followed by 40 cycles of 95° C. for 15 s and 60° C. for 1 min. The level of miRNA expression was measured using the threshold cycle (Ct), the fractional cycle number at which the fluorescence of each sample passes a fixed threshold. miRNA expression levels were normalized using an endogenous small RNA control U44 (Applied Biosystems). The expression of miRNA relative to small RNA U44 is reported as ΔCt, which was calculated by subtracting the Ct of U44 RNA from the Ct of target miRNA. 
     4. Results 
     MiRNA expression was analyzed between each group, Table 1 shows 523 miRNAs which have significant expression level, either over or less expression, between melanoma and nevi groups (p&lt;0.05). Table 2 showed 50 miRNAs with the most significant expression between the two groups. Table 3 showed 378 miRNAs which have significant expression differences between melanoma and normal skin groups (p&lt;0.05). Table 4 showed 50 miRNAs with the most significant expression differences between the two groups. Table 5 showed 174 miRNAs which have significant expression differences between melanoma and metastatic melanoma groups (p&lt;0.05). Table 6 showed 50 miRNAs with the most significant expression differences between the two groups. Table 7 showed 442 miRNAs which have significant expression differences between nevi and normal skin groups (p&lt;0.05). Table 8 showed 50 miRNAs with the most significant expression differences between the two groups. 
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 miRNAs significantly expressed between melanoma and nevi 
               
             
          
           
               
                   
                 Log2- 
                   
                   
                   
                 #Sample 
               
               
                 Probe Name 
                 FC 
                 P value 
                 AUC 
                 ProbeType 
                 Detected 
               
               
                   
               
             
          
           
               
                 miR-132 
                 2.98 
                  1.2728E−32 
                 0.994 
                 miRNA 
                 96 
               
               
                 miR-150 
                 3.27 
                 5.83192E−30 
                 1.000 
                 miRNA 
                 117 
               
               
                 miR-339-5p 
                 2.73 
                 1.69388E−27 
                 0.988 
                 miRNA 
                 75 
               
               
                 miR-15b 
                 3.02 
                 6.41784E−27 
                 0.978 
                 miRNA 
                 125 
               
               
                 miR-342-3p 
                 2.23 
                 7.03798E−26 
                 0.993 
                 miRNA 
                 136 
               
               
                 miR-572 
                 −2.78 
                 1.00008E−25 
                 0.970 
                 miRNA 
                 116 
               
               
                 miR-155 
                 4.14 
                 1.28454E−25 
                 0.981 
                 miRNA 
                 123 
               
               
                 miR-425 
                 2.74 
                 8.13135E−25 
                 0.975 
                 miRNA 
                 114 
               
               
                 miR-1202 
                 −2.63 
                 2.97795E−23 
                 0.986 
                 miRNA 
                 57 
               
               
                 miR-1268 
                 −2.68 
                 6.05905E−23 
                 0.997 
                 miRNA 
                 133 
               
               
                 HBII-382_s 
                 −1.71 
                 6.58931E−22 
                 0.971 
                 scaRna 
                 126 
               
               
                 miR-1225-5p 
                 −2.36 
                 1.05361E−21 
                 0.953 
                 miRNA 
                 90 
               
               
                 miR-30c 
                 2.39 
                 2.58594E−21 
                 0.980 
                 miRNA 
                 125 
               
               
                 miR-106b-star 
                 2.24 
                 3.48507E−21 
                 0.961 
                 miRNA 
                 72 
               
               
                 miR-125a-5p 
                 2.39 
                 6.90609E−21 
                 0.967 
                 miRNA 
                 128 
               
               
                 mgU6-53B 
                 −1.51 
                 1.74782E−20 
                 0.973 
                 CDBox 
                 99 
               
               
                 miR-25 
                 2.57 
                 1.52769E−19 
                 0.956 
                 miRNA 
                 118 
               
               
                 miR-149-star 
                 −2.03 
                 1.64858E−19 
                 1.000 
                 miRNA 
                 135 
               
               
                 miR-939 
                 −2.21 
                  1.9129E−19 
                 0.973 
                 miRNA 
                 57 
               
               
                 miR-92b-star 
                 −2.30 
                 2.05085E−19 
                 0.969 
                 miRNA 
                 111 
               
               
                 miR-500-star 
                 2.38 
                 3.11256E−19 
                 0.956 
                 miRNA 
                 97 
               
               
                 miR-22 
                 2.69 
                  3.8844E−19 
                 0.965 
                 miRNA 
                 120 
               
               
                 HBII-142_x 
                 −1.22 
                 4.93318E−19 
                 0.981 
                 CDBox 
                 135 
               
               
                 miR-181b 
                 2.41 
                 1.00639E−18 
                 0.960 
                 miRNA 
                 130 
               
               
                 HBII-142 
                 −1.33 
                 1.18794E−18 
                 0.988 
                 CDBox 
                 135 
               
               
                 U38B 
                 −1.95 
                 1.35964E−18 
                 0.975 
                 CDBox 
                 134 
               
               
                 miR-663 
                 −2.10 
                 2.04672E−18 
                 0.984 
                 miRNA 
                 134 
               
               
                 miR-1224-5p 
                 −2.54 
                 2.91169E−18 
                 0.946 
                 miRNA 
                 78 
               
               
                 miR-23a 
                 1.18 
                 2.99523E−18 
                 0.967 
                 miRNA 
                 137 
               
               
                 HBII-85-6_x 
                 −1.73 
                 4.78418E−18 
                 0.939 
                 CDBox 
                 137 
               
               
                 miR-1207-5p 
                 −2.14 
                 4.98991E−18 
                 0.995 
                 miRNA 
                 133 
               
               
                 miR-1301 
                 2.36 
                 5.22389E−18 
                 0.931 
                 miRNA 
                 54 
               
               
                 miR-1228-star 
                 −2.41 
                 5.51513E−18 
                 0.997 
                 miRNA 
                 134 
               
               
                 miR-345 
                 2.45 
                 6.43552E−18 
                 0.942 
                 miRNA 
                 75 
               
               
                 miR-30a-star 
                 2.32 
                 6.92165E−18 
                 0.932 
                 miRNA 
                 64 
               
               
                 ENSG00000199411_s 
                 −1.97 
                 7.23325E−18 
                 0.991 
                 snoRNA 
                 135 
               
               
                 ENSG00000202327 
                 −1.40 
                 8.75839E−18 
                 0.938 
                 snoRNA 
                 54 
               
               
                 miR-92a 
                 1.67 
                 9.49427E−18 
                 0.979 
                 miRNA 
                 136 
               
               
                 miR-127-3p 
                 2.43 
                 9.84615E−18 
                 0.936 
                 miRNA 
                 89 
               
               
                 HBII-85-26 
                 −2.07 
                 1.50619E−17 
                 0.951 
                 CDBox 
                 136 
               
               
                 miR-1308 
                 −2.14 
                 1.99888E−17 
                 0.999 
                 miRNA 
                 135 
               
               
                 miR-31 
                 3.29 
                 2.46697E−17 
                 0.913 
                 miRNA 
                 103 
               
               
                 miR-921 
                 −1.46 
                 2.61089E−17 
                 0.928 
                 miRNA 
                 49 
               
               
                 miR-146b-5p 
                 2.37 
                 6.10422E−17 
                 0.918 
                 miRNA 
                 83 
               
               
                 miR-768-3p 
                 −1.05 
                 6.66153E−17 
                 0.949 
                 miRNA 
                 137 
               
               
                 miR-708 
                 2.29 
                 6.86187E−17 
                 0.929 
                 miRNA 
                 102 
               
               
                 miR-139-5p 
                 2.23 
                 2.78962E−16 
                 0.922 
                 miRNA 
                 80 
               
               
                 ACA24_x 
                 1.33 
                 3.37973E−16 
                 0.925 
                 HAcaBox 
                 98 
               
               
                 miR-501-3p 
                 1.83 
                 4.76751E−16 
                 0.923 
                 miRNA 
                 80 
               
               
                 miR-502-3p 
                 2.16 
                 5.19509E−16 
                 0.925 
                 miRNA 
                 110 
               
               
                 miR-923 
                 −1.86 
                 5.55191E−16 
                 0.996 
                 miRNA 
                 135 
               
               
                 U94 
                 −1.17 
                 6.40118E−16 
                 0.926 
                 CDBox 
                 109 
               
               
                 miR-574-3p 
                 2.32 
                 8.68105E−16 
                 0.946 
                 miRNA 
                 122 
               
               
                 miR-135a-star 
                 −1.90 
                 1.03062E−15 
                 0.914 
                 miRNA 
                 44 
               
               
                 ENSG00000207098_x 
                 −1.03 
                 1.25346E−15 
                 0.924 
                 snoRNA 
                 86 
               
               
                 U38B_x 
                 −1.55 
                 2.54255E−15 
                 0.958 
                 CDBox 
                 133 
               
               
                 miR-423-3p 
                 1.83 
                 2.74508E−15 
                 0.932 
                 miRNA 
                 107 
               
               
                 miR-198 
                 −1.79 
                 3.57486E−15 
                 0.909 
                 miRNA 
                 31 
               
               
                 ACA16 
                 1.43 
                 4.07871E−15 
                 0.919 
                 HAcaBox 
                 47 
               
               
                 ACA25 
                 1.06 
                 4.62028E−15 
                 0.910 
                 HAcaBox 
                 73 
               
               
                 miR-769-5p 
                 1.63 
                 4.79679E−15 
                 0.920 
                 miRNA 
                 56 
               
               
                 ENSG00000212523_x 
                 −1.38 
                 6.22717E−15 
                 0.927 
                 snoRNA 
                 135 
               
               
                 mgU6-53B_x 
                 −0.99 
                 6.32629E−15 
                 0.919 
                 CDBox 
                 121 
               
               
                 Z17B 
                 0.97 
                  1.1349E−14 
                 0.903 
                 CDBox 
                 117 
               
               
                 U81_x 
                 1.22 
                 1.25935E−14 
                 0.913 
                 CDBox 
                 120 
               
               
                 miR-532-5p 
                 2.10 
                 1.27686E−14 
                 0.933 
                 miRNA 
                 113 
               
               
                 ENSG00000200879 
                 1.27 
                 1.49096E−14 
                 0.917 
                 snoRNA 
                 120 
               
               
                 HBII-419 
                 −1.07 
                 2.02184E−14 
                 0.938 
                 CDBox 
                 133 
               
               
                 U58B_x 
                 0.96 
                 9.25535E−14 
                 0.898 
                 CDBox 
                 132 
               
               
                 ENSG00000201619 
                 −1.66 
                 1.14582E−13 
                 0.911 
                 snoRNA 
                 62 
               
               
                 miR-28-3p 
                 2.15 
                 1.17402E−13 
                 0.900 
                 miRNA 
                 94 
               
               
                 miR-1300 
                 −1.86 
                 1.21666E−13 
                 0.892 
                 miRNA 
                 32 
               
               
                 miR-191 
                 1.18 
                  1.2767E−13 
                 0.983 
                 miRNA 
                 135 
               
               
                 miR-181a-2-star 
                 1.93 
                 2.91245E−13 
                 0.898 
                 miRNA 
                 99 
               
               
                 U38A 
                 −1.52 
                 3.04032E−13 
                 0.971 
                 CDBox 
                 134 
               
               
                 U59A 
                 −1.01 
                 3.86379E−13 
                 0.948 
                 CDBox 
                 135 
               
               
                 ENSG00000212397 
                 −1.21 
                 4.58111E−13 
                 0.902 
                 snoRNA 
                 134 
               
               
                 HBII-85-26_x 
                 −1.13 
                 4.73092E−13 
                 0.897 
                 CDBox 
                 135 
               
               
                 miR-638 
                 −1.62 
                 5.93273E−13 
                 0.967 
                 miRNA 
                 135 
               
               
                 miR-421 
                 1.84 
                 6.09941E−13 
                 0.881 
                 miRNA 
                 68 
               
               
                 miR-21 
                 2.62 
                 6.26336E−13 
                 0.888 
                 miRNA 
                 79 
               
               
                 miR-24-2-star 
                 1.81 
                 8.95923E−13 
                 0.879 
                 miRNA 
                 63 
               
               
                 U36C 
                 −0.92 
                  1.1248E−12 
                 0.931 
                 CDBox 
                 135 
               
               
                 miR-92b 
                 1.43 
                 1.15239E−12 
                 0.901 
                 miRNA 
                 91 
               
               
                 miR-199a-5p 
                 1.69 
                 1.22362E−12 
                 0.901 
                 miRNA 
                 129 
               
               
                 ACA24_s 
                 1.36 
                 1.86137E−12 
                 0.880 
                 HAcaBox 
                 128 
               
               
                 ACA9 
                 1.36 
                 1.92813E−12 
                 0.888 
                 HAcaBox 
                 82 
               
               
                 ENSG00000199411_x 
                 −0.87 
                 2.22903E−12 
                 0.868 
                 snoRNA 
                 132 
               
               
                 ENSG00000199435 
                 −0.94 
                 2.45067E−12 
                 0.903 
                 snoRNA 
                 63 
               
               
                 miR-182 
                 1.80 
                 3.73608E−12 
                 0.905 
                 miRNA 
                 113 
               
               
                 miR-1275 
                 −1.56 
                 3.97629E−12 
                 0.888 
                 miRNA 
                 127 
               
               
                 miR-150-star 
                 −1.70 
                 4.59839E−12 
                 0.897 
                 miRNA 
                 48 
               
               
                 ACA48_x 
                 1.08 
                 5.05927E−12 
                 0.881 
                 HAcaBox 
                 130 
               
               
                 HBII-85-8_x 
                 −1.09 
                 5.47576E−12 
                 0.877 
                 CDBox 
                 135 
               
               
                 miR-99b 
                 1.33 
                 5.48764E−12 
                 0.907 
                 miRNA 
                 129 
               
               
                 U74_x 
                 −1.00 
                 6.23625E−12 
                 0.941 
                 CDBox 
                 135 
               
               
                 miR-1271 
                 1.71 
                  6.2815E−12 
                 0.885 
                 miRNA 
                 70 
               
               
                 ENSG00000206637_x 
                 −0.86 
                 6.43179E−12 
                 0.878 
                 snoRNA 
                 59 
               
               
                 miR-20b 
                 2.16 
                 1.13294E−11 
                 0.892 
                 miRNA 
                 99 
               
               
                 ENSG00000200652 
                 −0.93 
                 1.18064E−11 
                 0.876 
                 snoRNA 
                 40 
               
               
                 U13 
                 −0.94 
                 1.19458E−11 
                 0.899 
                 CDBox 
                 135 
               
               
                 ENSG00000201660 
                 −1.13 
                 1.27245E−11 
                 0.903 
                 snoRNA 
                 132 
               
               
                 HBII-85-23_x 
                 1.18 
                 1.36679E−11 
                 0.883 
                 CDBox 
                 58 
               
               
                 miR-149 
                 1.85 
                 1.38908E−11 
                 0.896 
                 miRNA 
                 100 
               
               
                 ACA9_x 
                 1.14 
                 2.05352E−11 
                 0.877 
                 HAcaBox 
                 77 
               
               
                 miR-1234 
                 −1.10 
                 2.16855E−11 
                 0.856 
                 miRNA 
                 68 
               
               
                 miR-1180 
                 1.50 
                 2.61371E−11 
                 0.878 
                 miRNA 
                 37 
               
               
                 miR-30a 
                 1.80 
                 3.39494E−11 
                 0.861 
                 miRNA 
                 113 
               
               
                 U44_x 
                 1.13 
                 3.71768E−11 
                 0.953 
                 CDBox 
                 135 
               
               
                 miR-181c 
                 1.54 
                 4.41605E−11 
                 0.861 
                 miRNA 
                 59 
               
               
                 ENSG00000202498_x 
                 −1.07 
                 4.69703E−11 
                 0.855 
                 snoRNA 
                 137 
               
               
                 miR-940 
                 −1.22 
                 5.55177E−11 
                 0.861 
                 miRNA 
                 53 
               
               
                 miR-500 
                 1.76 
                 6.23662E−11 
                 0.854 
                 miRNA 
                 86 
               
               
                 ENSG00000212627 
                 −0.78 
                 6.92771E−11 
                 0.871 
                 snoRNA 
                 69 
               
               
                 ENSG00000207027 
                 −0.84 
                 8.23933E−11 
                 0.879 
                 snoRNA 
                 36 
               
               
                 miR-27a-star 
                 1.63 
                 8.66657E−11 
                 0.856 
                 miRNA 
                 40 
               
               
                 miR-128 
                 1.66 
                 9.46068E−11 
                 0.858 
                 miRNA 
                 44 
               
               
                 snR38C 
                 −1.01 
                 9.50785E−11 
                 0.926 
                 CDBox 
                 134 
               
               
                 ENSG00000212266 
                 −0.93 
                  9.6633E−11 
                 0.848 
                 snoRNA 
                 93 
               
               
                 miR-185 
                 1.76 
                 1.06661E−10 
                 0.922 
                 miRNA 
                 132 
               
               
                 ACA64 
                 −0.97 
                 1.08821E−10 
                 0.858 
                 HAcaBox 
                 33 
               
               
                 miR-151-3p 
                 1.71 
                 1.37187E−10 
                 0.884 
                 miRNA 
                 117 
               
               
                 miR-130b 
                 2.12 
                 1.55732E−10 
                 0.870 
                 miRNA 
                 90 
               
               
                 miR-27b-star 
                 1.70 
                 1.72122E−10 
                 0.849 
                 miRNA 
                 51 
               
               
                 miR-665 
                 −1.43 
                 1.78835E−10 
                 0.853 
                 miRNA 
                 47 
               
               
                 miR-18a 
                 2.01 
                 1.91068E−10 
                 0.854 
                 miRNA 
                 77 
               
               
                 ACA36_x 
                 −1.03 
                  2.0756E−10 
                 0.875 
                 HAcaBox 
                 88 
               
               
                 ENS00000212432_s 
                 −0.94 
                 2.08962E−10 
                 0.882 
                 snoRNA 
                 75 
               
               
                 ENSG00000202093_x 
                 1.01 
                 4.04338E−10 
                 0.873 
                 snoRNA 
                 125 
               
               
                 miR-487b 
                 1.41 
                 4.22069E−10 
                 0.848 
                 miRNA 
                 58 
               
               
                 U65 
                 −0.98 
                 4.31315E−10 
                 0.847 
                 HAcaBox 
                 128 
               
               
                 miR-532-3p 
                 1.19 
                 4.73885E−10 
                 0.881 
                 miRNA 
                 95 
               
               
                 U101 
                 −0.79 
                 5.89958E−10 
                 0.845 
                 CDBox 
                 134 
               
               
                 miR-138 
                 2.09 
                 6.68933E−10 
                 0.839 
                 miRNA 
                 82 
               
               
                 HBII-99 
                 1.01 
                 7.89663E−10 
                 0.847 
                 CDBox 
                 122 
               
               
                 miR-222 
                 1.12 
                 9.21171E−10 
                 0.913 
                 miRNA 
                 135 
               
               
                 miR-652 
                 1.46 
                 9.82734E−10 
                 0.862 
                 miRNA 
                 119 
               
               
                 miR-10a 
                 1.82 
                 9.97674E−10 
                 0.843 
                 miRNA 
                 69 
               
               
                 miR-625 
                 1.46 
                 1.06062E−09 
                 0.833 
                 miRNA 
                 59 
               
               
                 ENSG00000212182 
                 −0.89 
                 1.13997E−09 
                 0.828 
                 snoRNA 
                 59 
               
               
                 miR-331-3p 
                 1.47 
                 1.22124E−09 
                 0.834 
                 miRNA 
                 64 
               
               
                 miR-1281 
                 −1.46 
                 1.31027E−09 
                 0.843 
                 miRNA 
                 137 
               
               
                 miR-28-5p 
                 1.53 
                 1.35526E−09 
                 0.863 
                 miRNA 
                 105 
               
               
                 ACA23 
                 0.76 
                 2.45172E−09 
                 0.837 
                 HAcaBox 
                 113 
               
               
                 U27 
                 1.04 
                 2.55698E−09 
                 0.874 
                 CDBox 
                 134 
               
               
                 miR-339-3p 
                 1.12 
                  2.9234E−09 
                 0.836 
                 miRNA 
                 60 
               
               
                 U68_x 
                 1.00 
                 3.99716E−09 
                 0.861 
                 HAcaBox 
                 133 
               
               
                 miR-92a-2-star 
                 −1.06 
                 4.67209E−09 
                 0.830 
                 miRNA 
                 46 
               
               
                 miR-107 
                 1.08 
                 5.05068E−09 
                 0.946 
                 miRNA 
                 135 
               
               
                 U104 
                 −0.79 
                 5.07142E−09 
                 0.918 
                 CDBox 
                 135 
               
               
                 miR-671-5p 
                 −1.16 
                 6.05404E−09 
                 0.877 
                 miRNA 
                 69 
               
               
                 miR-193b 
                 1.22 
                 6.27171E−09 
                 0.852 
                 miRNA 
                 130 
               
               
                 miR-1272 
                 −1.13 
                 6.29028E−09 
                 0.826 
                 miRNA 
                 67 
               
               
                 miR-221 
                 1.23 
                  6.5716E−09 
                 0.911 
                 miRNA 
                 135 
               
               
                 14qll-14 
                 1.27 
                  6.8516E−09 
                 0.828 
                 CDBox 
                 56 
               
               
                 miR-1288 
                 −0.92 
                 6.95468E−09 
                 0.804 
                 miRNA 
                 43 
               
               
                 ENSG00000212458 
                 −0.80 
                 7.62772E−09 
                 0.853 
                 snoRNA 
                 95 
               
               
                 miR-584 
                 1.61 
                 7.63622E−09 
                 0.815 
                 miRNA 
                 57 
               
               
                 U106 
                 0.64 
                 8.00299E−09 
                 0.821 
                 CDBox 
                 112 
               
               
                 miR-152 
                 1.58 
                 8.26552E−09 
                 0.885 
                 miRNA 
                 122 
               
               
                 ENSG00000212551 
                 −0.72 
                 9.08023E−09 
                 0.841 
                 snoRNA 
                 73 
               
               
                 U96a_x 
                 −0.89 
                 1.00143E−08 
                 0.863 
                 CDBox 
                 134 
               
               
                 ENSG00000212139_x 
                 −0.74 
                 1.22887E−08 
                 0.811 
                 snoRNA 
                 124 
               
               
                 miR-409-3p 
                 1.14 
                 1.25923E−08 
                 0.832 
                 miRNA 
                 92 
               
               
                 miR-214-star 
                 1.59 
                 1.34912E−08 
                 0.830 
                 miRNA 
                 47 
               
               
                 ACA20 
                 0.91 
                 1.36416E−08 
                 0.873 
                 HAcaBox 
                 131 
               
               
                 HBII-82 
                 −0.73 
                 1.52532E−08 
                 0.823 
                 CDBox 
                 90 
               
               
                 U49A 
                 −0.86 
                 1.55769E−08 
                 0.856 
                 CDBox 
                 135 
               
               
                 miR-362-5p 
                 1.63 
                 1.67441E−08 
                 0.824 
                 miRNA 
                 94 
               
               
                 ACA40_x 
                 1.17 
                 1.68127E−08 
                 0.872 
                 HAcaBox 
                 134 
               
               
                 U63 
                 −0.98 
                 1.83203E−08 
                 0.916 
                 CDBox 
                 135 
               
               
                 HBII-436 
                 0.76 
                 1.85498E−08 
                 0.810 
                 CDBox 
                 102 
               
               
                 miR-15a 
                 1.74 
                  1.8639E−08 
                 0.826 
                 miRNA 
                 80 
               
               
                 U103_s 
                 0.83 
                 2.02002E−08 
                 0.809 
                 CDBox 
                 111 
               
               
                 miR-1185 
                 −0.96 
                 2.35573E−08 
                 0.822 
                 miRNA 
                 42 
               
               
                 ACA15_s 
                 0.74 
                 2.84086E−08 
                 0.812 
                 HAcaBox 
                 116 
               
               
                 miR-30b 
                 1.37 
                 3.22874E−08 
                 0.843 
                 miRNA 
                 128 
               
               
                 spike_in-control-21 
                 −0.70 
                 3.25874E−08 
                 0.832 
                 Oligonucleotide 
                 42 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 U15B 
                 0.71 
                 3.51612E−08 
                 0.825 
                 CDBox 
                 129 
               
               
                 miR-143 
                 1.99 
                 3.72999E−08 
                 0.820 
                 miRNA 
                 126 
               
               
                 U67_x 
                 0.79 
                 4.12783E−08 
                 0.813 
                 HAcaBox 
                 50 
               
               
                 miR-10b 
                 1.41 
                 4.29563E−08 
                 0.821 
                 miRNA 
                 106 
               
               
                 U99 
                 −0.65 
                 4.50576E−08 
                 0.817 
                 HAcaBox 
                 137 
               
               
                 miR-194 
                 1.27 
                 5.18787E−08 
                 0.813 
                 miRNA 
                 54 
               
               
                 miR-324-5p 
                 1.14 
                 5.23291E−08 
                 0.804 
                 miRNA 
                 102 
               
               
                 miR-125b-2-star 
                 1.19 
                 5.54514E−08 
                 0.819 
                 miRNA 
                 58 
               
               
                 HBII-52-37_x 
                 −0.76 
                 8.93997E−08 
                 0.805 
                 CDBox 
                 37 
               
               
                 ACA48 
                 0.72 
                 9.13165E−08 
                 0.825 
                 HAcaBox 
                 121 
               
               
                 miR-103 
                 0.97 
                 9.25296E−08 
                 0.931 
                 miRNA 
                 135 
               
               
                 miR-342-5p 
                 1.32 
                 9.27998E−08 
                 0.827 
                 miRNA 
                 98 
               
               
                 miR-93 
                 1.08 
                 9.93604E−08 
                 0.896 
                 miRNA 
                 134 
               
               
                 miR-296-3p 
                 −1.00 
                 1.10115E−07 
                 0.818 
                 miRNA 
                 90 
               
               
                 miR-200b-star 
                 1.30 
                 1.13507E−07 
                 0.791 
                 miRNA 
                 74 
               
               
                 U44 
                 0.87 
                 1.17791E−07 
                 0.897 
                 CDBox 
                 134 
               
               
                 ENSG00000207016_x 
                 −0.63 
                 1.23122E−07 
                 0.803 
                 snoRNA 
                 48 
               
               
                 spike_in-control-31 
                 −0.14 
                 1.24357E−07 
                 0.798 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 U68 
                 0.83 
                 1.72086E−07 
                 0.856 
                 HAcaBox 
                 130 
               
               
                 U24 
                 −0.55 
                 2.39582E−07 
                 0.822 
                 CDBox 
                 133 
               
               
                 U83 
                 0.62 
                 2.42808E−07 
                 0.846 
                 CDBox 
                 135 
               
               
                 ENSG00000212273_x 
                 −0.80 
                 2.45728E−07 
                 0.811 
                 snoRNA 
                 126 
               
               
                 14qll-14_x 
                 1.03 
                 2.59298E−07 
                 0.797 
                 CDBox 
                 56 
               
               
                 miR-660 
                 1.26 
                 2.65402E−07 
                 0.788 
                 miRNA 
                 71 
               
               
                 14qll-7 
                 −0.70 
                 2.89281E−07 
                 0.762 
                 CDBox 
                 77 
               
               
                 U78_s 
                 1.01 
                 3.07849E−07 
                 0.836 
                 CDBox 
                 135 
               
               
                 miR-744 
                 −1.03 
                 3.11141E−07 
                 0.818 
                 miRNA 
                 133 
               
               
                 U22 
                 −0.54 
                 3.14269E−07 
                 0.793 
                 CDBox 
                 135 
               
               
                 ACA11 
                 0.70 
                 3.50307E−07 
                 0.793 
                 HAcaBox 
                 48 
               
               
                 ENSG00000212553_x 
                 −0.69 
                 3.51184E−07 
                 0.794 
                 snoRNA 
                 60 
               
               
                 U67 
                 0.76 
                 3.78503E−07 
                 0.796 
                 HAcaBox 
                 44 
               
               
                 miR-382 
                 1.35 
                 3.83783E−07 
                 0.787 
                 miRNA 
                 47 
               
               
                 miR-212 
                 1.58 
                 3.89653E−07 
                 0.854 
                 miRNA 
                 62 
               
               
                 miR-93-star 
                 1.26 
                 4.02443E−07 
                 0.808 
                 miRNA 
                 48 
               
               
                 U18C_x 
                 0.74 
                  4.1571E−07 
                 0.794 
                 CDBox 
                 74 
               
               
                 miR-1274a 
                 1.22 
                  4.1577E−07 
                 0.788 
                 miRNA 
                 48 
               
               
                 ACA25_x 
                 0.77 
                 4.42948E−07 
                 0.799 
                 HAcaBox 
                 114 
               
               
                 U15A 
                 −0.74 
                 4.57833E−07 
                 0.821 
                 CDBox 
                 128 
               
               
                 14ql-1 
                 −0.77 
                 5.08646E−07 
                 0.784 
                 CDBox 
                 70 
               
               
                 ENSG00000201816 
                 −0.60 
                 5.51521E−07 
                 0.789 
                 snoRNA 
                 49 
               
               
                 miR-145 
                 1.56 
                 6.30644E−07 
                 0.775 
                 miRNA 
                 135 
               
               
                 HBII-420 
                 −0.75 
                 7.96309E−07 
                 0.828 
                 CDBox 
                 131 
               
               
                 ACA2b 
                 −0.66 
                 8.56351E−07 
                 0.787 
                 HAcaBox 
                 47 
               
               
                 miR-1273 
                 −0.94 
                 1.01224E−06 
                 0.768 
                 miRNA 
                 47 
               
               
                 miR-641 
                 −0.85 
                  1.0165E−06 
                 0.765 
                 miRNA 
                 39 
               
               
                 ENSG00000202216 
                 −0.60 
                 1.02726E−06 
                 0.778 
                 snoRNA 
                 53 
               
               
                 U97 
                 −0.72 
                 1.06795E−06 
                 0.794 
                 CDBox 
                 133 
               
               
                 EN8G00000200706_x 
                 −0.66 
                 1.06937E−06 
                 0.778 
                 snoRNA 
                 43 
               
               
                 miR-363 
                 1.33 
                 1.20135E−06 
                 0.806 
                 miRNA 
                 44 
               
               
                 ENSG00000201348 
                 −0.48 
                 1.23741E−06 
                 0.779 
                 snoRNA 
                 94 
               
               
                 U83A 
                 0.75 
                 1.32496E−06 
                 0.818 
                 CDBox 
                 127 
               
               
                 miR-197 
                 1.27 
                  1.3431E−06 
                 0.807 
                 miRNA 
                 103 
               
               
                 miR-106b 
                 1.23 
                 1.49024E−06 
                 0.805 
                 miRNA 
                 127 
               
               
                 HBII-180A_x 
                 0.60 
                 1.62491E−06 
                 0.784 
                 CDBox 
                 123 
               
               
                 snR38B 
                 −0.76 
                 1.68966E−06 
                 0.803 
                 CDBox 
                 126 
               
               
                 snR38A 
                 −0.87 
                 1.99208E−06 
                 0.838 
                 CDBox 
                 130 
               
               
                 miR-134 
                 1.15 
                 2.09717E−06 
                 0.774 
                 miRNA 
                 61 
               
               
                 miR-29b-1-star 
                 1.41 
                 2.20964E−06 
                 0.764 
                 miRNA 
                 46 
               
               
                 mgh18S-121 
                 −0.66 
                 2.37986E−06 
                 0.833 
                 CDBox 
                 134 
               
               
                 mgU6-77 
                 0.59 
                 2.38866E−06 
                 0.742 
                 CDBox 
                 129 
               
               
                 HBII-52-25_x 
                 −0.60 
                 2.44738E−06 
                 0.767 
                 CDBox 
                 57 
               
               
                 U48 
                 0.83 
                 2.51688E−06 
                 0.777 
                 CDBox 
                 129 
               
               
                 14qll-19 
                 −0.73 
                 2.52816E−06 
                 0.770 
                 CDBox 
                 43 
               
               
                 miR-552 
                 −0.66 
                 2.70576E−06 
                 0.760 
                 miRNA 
                 34 
               
               
                 miR-424-star 
                 1.31 
                 2.79344E−06 
                 0.762 
                 miRNA 
                 41 
               
               
                 HBII-316 
                 −0.60 
                 3.06016E−06 
                 0.795 
                 CDBox 
                 130 
               
               
                 U35B 
                 −0.64 
                 3.21695E−06 
                 0.811 
                 CDBox 
                 128 
               
               
                 miR-30d 
                 1.02 
                 3.25088E−06 
                 0.817 
                 miRNA 
                 131 
               
               
                 miR-1248 
                 −0.72 
                 3.27937E−06 
                 0.746 
                 miRNA 
                 39 
               
               
                 ENSG00000212579_x 
                 −0.65 
                 3.55239E−06 
                 0.758 
                 snoRNA 
                 100 
               
               
                 hsa-let-7b 
                 0.90 
                 3.76667E−06 
                 0.919 
                 miRNA 
                 136 
               
               
                 miR-29b-2-star 
                 −0.78 
                 3.86015E−06 
                 0.760 
                 miRNA 
                 126 
               
               
                 miR-148a 
                 1.04 
                 4.16979E−06 
                 0.773 
                 miRNA 
                 41 
               
               
                 miR-629 
                 1.16 
                 4.35623E−06 
                 0.776 
                 miRNA 
                 64 
               
               
                 HBII-295 
                 −0.58 
                 4.36224E−06 
                 0.767 
                 CDBox 
                 128 
               
               
                 miR-877 
                 −1.00 
                 4.43293E−06 
                 0.794 
                 miRNA 
                 126 
               
               
                 ACA62 
                 −0.61 
                 4.43469E−06 
                 0.774 
                 HAcaBox 
                 88 
               
               
                 ENSG00000207100_x 
                 −0.50 
                 4.49659E−06 
                 0.765 
                 snoRNA 
                 72 
               
               
                 HBII-180C 
                 0.62 
                 4.78509E−06 
                 0.780 
                 CDBox 
                 115 
               
               
                 HBII-115 
                 −0.57 
                 5.03448E−06 
                 0.768 
                 CDBox 
                 117 
               
               
                 miR-192 
                 1.14 
                 5.73989E−06 
                 0.763 
                 miRNA 
                 32 
               
               
                 miR-505-star 
                 1.23 
                 5.74055E−06 
                 0.762 
                 miRNA 
                 58 
               
               
                 spike_in-control-7 
                 −0.80 
                 5.89851E−06 
                 0.790 
                 Oligonucleotide 
                 54 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 miR-422a 
                 1.17 
                 5.96437E−06 
                 0.782 
                 miRNA 
                 84 
               
               
                 U83B 
                 −0.56 
                 6.55847E−06 
                 0.842 
                 CDBox 
                 135 
               
               
                 ENSG00000212134_x 
                 −0.65 
                 6.92676E−06 
                 0.752 
                 snoRNA 
                 88 
               
               
                 U50B_x 
                 −0.67 
                 7.13703E−06 
                 0.783 
                 CDBox 
                 135 
               
               
                 miR-193a-3p 
                 0.91 
                 8.82408E−06 
                 0.747 
                 miRNA 
                 42 
               
               
                 ENSG00000201848 
                 −0.62 
                 9.39369E−06 
                 0.759 
                 snoRNA 
                 34 
               
               
                 miR-148b 
                 0.93 
                 9.94696E−06 
                 0.757 
                 miRNA 
                 32 
               
               
                 miR-483-5p 
                 −1.10 
                  1.1191E−05 
                 0.756 
                 miRNA 
                 48 
               
               
                 ACA58_x 
                 0.62 
                 1.17327E−05 
                 0.766 
                 HAcaBox 
                 89 
               
               
                 miR-1260 
                 0.94 
                 1.21296E−05 
                 0.776 
                 miRNA 
                 120 
               
               
                 U108_x 
                 −0.50 
                 1.23481E−05 
                 0.763 
                 HAcaBox 
                 87 
               
               
                 ACA61 
                 −0.60 
                 1.27104E−05 
                 0.839 
                 HAcaBox 
                 135 
               
               
                 14q-0 
                 −0.58 
                 1.39356E−05 
                 0.767 
                 CDBox 
                 49 
               
               
                 ACA16_x 
                 0.55 
                 1.40972E−05 
                 0.763 
                 HAcaBox 
                 98 
               
               
                 miR-30b-star 
                 1.22 
                 1.61096E−05 
                 0.755 
                 miRNA 
                 61 
               
               
                 ENSG00000201848_x 
                 −0.58 
                 2.11059E−05 
                 0.740 
                 snoRNA 
                 40 
               
               
                 U3-2_s 
                 −0.57 
                 2.19611E−05 
                 0.800 
                 CDBox 
                 135 
               
               
                 spike_in-control-2 
                 −0.31 
                 2.25868E−05 
                 0.747 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 miR-933 
                 −0.56 
                 2.89505E−05 
                 0.776 
                 miRNA 
                 100 
               
               
                 miR-181d 
                 1.03 
                 2.90988E−05 
                 0.750 
                 miRNA 
                 61 
               
               
                 HBII-239 
                 0.45 
                 2.92435E−05 
                 0.744 
                 CDBox 
                 135 
               
               
                 miR-497 
                 1.17 
                 2.92719E−05 
                 0.730 
                 miRNA 
                 104 
               
               
                 HBII-251 
                 −0.48 
                 3.04823E−05 
                 0.797 
                 CDBox 
                 135 
               
               
                 U51 
                 −0.73 
                 3.10371E−05 
                 0.809 
                 CDBox 
                 134 
               
               
                 miR-211 
                 1.45 
                 3.41149E−05 
                 0.759 
                 miRNA 
                 84 
               
               
                 miR-29a 
                 1.10 
                 3.68833E−05 
                 0.777 
                 miRNA 
                 128 
               
               
                 ENSG00000212538 
                 −0.54 
                 3.71864E−05 
                 0.746 
                 snoRNA 
                 35 
               
               
                 miR-886-3p 
                 1.11 
                 4.28603E−05 
                 0.711 
                 miRNA 
                 110 
               
               
                 miR-361-5p 
                 0.58 
                 4.51572E−05 
                 0.859 
                 miRNA 
                 135 
               
               
                 U51_x 
                 −0.48 
                  4.6523E−05 
                 0.773 
                 CDBox 
                 130 
               
               
                 U46 
                 0.59 
                 4.67792E−05 
                 0.744 
                 CDBox 
                 133 
               
               
                 HBII-85-11 
                 0.73 
                 4.72933E−05 
                 0.743 
                 CDBox 
                 31 
               
               
                 ENSG00000207410_x 
                 −0.51 
                 4.76931E−05 
                 0.731 
                 snoRNA 
                 55 
               
               
                 ENSG00000200492 
                 −0.51 
                 5.13688E−05 
                 0.756 
                 snoRNA 
                 60 
               
               
                 miR-503 
                 1.05 
                 5.22927E−05 
                 0.730 
                 miRNA 
                 41 
               
               
                 HBII-85-21_x 
                 0.68 
                 5.24419E−05 
                 0.749 
                 CDBox 
                 39 
               
               
                 miR-506 
                 1.27 
                 5.49222E−05 
                 0.750 
                 miRNA 
                 76 
               
               
                 ENSG00000212206_x 
                 −0.55 
                 5.68573E−05 
                 0.730 
                 snoRNA 
                 74 
               
               
                 U64 
                 0.49 
                  5.7913E−05 
                 0.755 
                 HAcaBox 
                 101 
               
               
                 miR-629-star 
                 1.07 
                 5.89793E−05 
                 0.745 
                 miRNA 
                 46 
               
               
                 ENSG00000212284 
                 −0.62 
                 6.56826E−05 
                 0.740 
                 snoRNA 
                 89 
               
               
                 ENSG00000212423_x 
                 −0.60 
                 6.59628E−05 
                 0.728 
                 snoRNA 
                 115 
               
               
                 U14B_x 
                 0.58 
                 6.71139E−05 
                 0.714 
                 CDBox 
                 53 
               
               
                 miR-324-3p 
                 0.78 
                 6.71385E−05 
                 0.766 
                 miRNA 
                 100 
               
               
                 miR-1287 
                 0.79 
                 7.37546E−05 
                 0.733 
                 miRNA 
                 40 
               
               
                 ENSG00000200897 
                 −0.49 
                 7.55587E−05 
                 0.730 
                 snoRNA 
                 38 
               
               
                 miR-498 
                 −0.68 
                 8.15427E−05 
                 0.730 
                 miRNA 
                 56 
               
               
                 U80 
                 0.53 
                 8.25012E−05 
                 0.750 
                 CDBox 
                 132 
               
               
                 14ql-8_x 
                 −0.55 
                 8.60271E−05 
                 0.740 
                 CDBox 
                 74 
               
               
                 miR-181a 
                 0.82 
                 8.99722E−05 
                 0.766 
                 miRNA 
                 134 
               
               
                 HBII-166 
                 −0.46 
                 9.07119E−05 
                 0.782 
                 CDBox 
                 134 
               
               
                 miR-181a-star 
                 1.03 
                 9.30754E−05 
                 0.727 
                 miRNA 
                 35 
               
               
                 U17b 
                 0.85 
                 9.69173E−05 
                 0.854 
                 HAcaBox 
                 135 
               
               
                 U55 
                 0.61 
                 0.000102241 
                 0.749 
                 CDBox 
                 135 
               
               
                 ENSG00000207407 
                 −0.43 
                 0.000115453 
                 0.706 
                 snoRNA 
                 56 
               
               
                 ENSG00000212206 
                 −0.50 
                 0.000123184 
                 0.701 
                 snoRNA 
                 46 
               
               
                 miR-489 
                 0.86 
                 0.000125451 
                 0.724 
                 miRNA 
                 58 
               
               
                 hsa-let-7g 
                 1.09 
                 0.000128044 
                 0.820 
                 miRNA 
                 127 
               
               
                 ENSG00000199363 
                 0.63 
                 0.000135904 
                 0.722 
                 snoRNA 
                 42 
               
               
                 miR-27a 
                 0.72 
                 0.000140934 
                 0.687 
                 miRNA 
                 136 
               
               
                 ACA32 
                 −0.40 
                 0.000144838 
                 0.746 
                 HAcaBox 
                 135 
               
               
                 ENSG00000207022 
                 −0.51 
                 0.000149628 
                 0.733 
                 snoRNA 
                 36 
               
               
                 miR-371-5p 
                 −0.86 
                 0.000152576 
                 0.706 
                 miRNA 
                 64 
               
               
                 miR-559 
                 −0.73 
                 0.000161415 
                 0.728 
                 miRNA 
                 72 
               
               
                 miR-193a-5p 
                 0.92 
                 0.000163281 
                 0.777 
                 miRNA 
                 121 
               
               
                 U23 
                 0.46 
                 0.00016459 
                 0.724 
                 HAcaBox 
                 127 
               
               
                 miR-196a-star 
                 −0.69 
                 0.000167433 
                 0.695 
                 miRNA 
                 37 
               
               
                 U52 
                 0.47 
                 0.00016782 
                 0.770 
                 CDBox 
                 135 
               
               
                 ACA38 
                 0.50 
                 0.000170509 
                 0.713 
                 HAcaBox 
                 42 
               
               
                 miR-30e-star 
                 0.82 
                 0.000173654 
                 0.736 
                 miRNA 
                 35 
               
               
                 U49A_x 
                 −0.61 
                 0.000183863 
                 0.785 
                 CDBox 
                 135 
               
               
                 U49B_x 
                 −0.54 
                 0.000189603 
                 0.729 
                 CDBox 
                 122 
               
               
                 U71a 
                 0.50 
                 0.000191925 
                 0.721 
                 HAcaBox 
                 50 
               
               
                 HBII-95 
                 0.42 
                 0.000194646 
                 0.724 
                 CDBox 
                 78 
               
               
                 ENSG00000212587 
                 −0.55 
                 0.000201583 
                 0.711 
                 snoRNA 
                 45 
               
               
                 ENSG00000212401 
                 −0.45 
                 0.000208666 
                 0.714 
                 snoRNA 
                 41 
               
               
                 HBII-296A 
                 −0.45 
                 0.000214033 
                 0.713 
                 CDBox 
                 78 
               
               
                 ACA67_x 
                 0.49 
                 0.000214513 
                 0.730 
                 HAcaBox 
                 54 
               
               
                 miR-494 
                 0.79 
                 0.000221314 
                 0.725 
                 miRNA 
                 135 
               
               
                 miR-17 
                 0.77 
                 0.00024803 
                 0.782 
                 miRNA 
                 134 
               
               
                 HBII-85-4_x 
                 −0.49 
                 0.000264241 
                 0.714 
                 CDBox 
                 125 
               
               
                 miR-16 
                 0.67 
                 0.000270072 
                 0.819 
                 miRNA 
                 135 
               
               
                 miR-135b-star 
                 −0.68 
                 0.000271929 
                 0.710 
                 miRNA 
                 38 
               
               
                 ACA37_x 
                 0.47 
                 0.00028645 
                 0.718 
                 HAcaBox 
                 84 
               
               
                 miR-126 
                 0.82 
                 0.000326027 
                 0.780 
                 miRNA 
                 133 
               
               
                 miR-200b 
                 0.93 
                 0.000339327 
                 0.714 
                 miRNA 
                 109 
               
               
                 miR-320d 
                 0.89 
                 0.000373646 
                 0.811 
                 miRNA 
                 134 
               
               
                 U100 
                 0.41 
                 0.000374728 
                 0.702 
                 scaRna 
                 80 
               
               
                 HBII-85-27_x 
                 −0.40 
                 0.000383642 
                 0.706 
                 CDBox 
                 56 
               
               
                 HBII-85-15_x 
                 0.60 
                 0.000397454 
                 0.706 
                 CDBox 
                 49 
               
               
                 miR-885-3p 
                 −1.11 
                 0.000407264 
                 0.692 
                 miRNA 
                 73 
               
               
                 miR-23b-star 
                 0.76 
                 0.000423736 
                 0.723 
                 miRNA 
                 52 
               
               
                 ENSG00000206909_x 
                 −0.46 
                 0.000424153 
                 0.710 
                 snoRNA 
                 47 
               
               
                 U92 
                 −0.50 
                 0.000428521 
                 0.750 
                 scaRna 
                 129 
               
               
                 miR-628-3p 
                 0.86 
                 0.000458761 
                 0.702 
                 miRNA 
                 38 
               
               
                 miR-27b 
                 0.70 
                 0.00047114 
                 0.718 
                 miRNA 
                 133 
               
               
                 U88 
                 −0.43 
                 0.000500023 
                 0.708 
                 scaRna 
                 69 
               
               
                 miR-130a 
                 1.00 
                 0.000518644 
                 0.729 
                 miRNA 
                 122 
               
               
                 ACA4 
                 0.42 
                 0.00055437 
                 0.673 
                 HAcaBox 
                 117 
               
               
                 miR-151-5p 
                 0.60 
                 0.00055594 
                 0.790 
                 miRNA 
                 135 
               
               
                 ENSG00000200394 
                 0.46 
                 0.000561813 
                 0.692 
                 snoRNA 
                 119 
               
               
                 ACA5 
                 0.48 
                 0.000585113 
                 0.708 
                 HAcaBox 
                 85 
               
               
                 miR-1285 
                 −0.74 
                 0.000607741 
                 0.698 
                 miRNA 
                 54 
               
               
                 miR-664-star 
                 −0.82 
                 0.000652475 
                 0.686 
                 miRNA 
                 96 
               
               
                 U56 
                 −0.57 
                 0.000672557 
                 0.787 
                 CDBox 
                 135 
               
               
                 ENSG00000200307 
                 −0.44 
                 0.000679263 
                 0.686 
                 snoRNA 
                 86 
               
               
                 miR-18b 
                 0.81 
                 0.000708619 
                 0.688 
                 miRNA 
                 32 
               
               
                 U76 
                 0.38 
                 0.000723275 
                 0.712 
                 CDBox 
                 135 
               
               
                 miR-378 
                 0.75 
                 0.000726542 
                 0.769 
                 miRNA 
                 131 
               
               
                 ENSG00000200307_x 
                 −0.43 
                 0.000739867 
                 0.716 
                 snoRNA 
                 50 
               
               
                 miR-513b 
                 1.02 
                 0.000775353 
                 0.701 
                 miRNA 
                 64 
               
               
                 U56_x 
                 0.54 
                 0.000779671 
                 0.716 
                 CDBox 
                 134 
               
               
                 miR-642 
                 −0.59 
                 0.000808799 
                 0.695 
                 miRNA 
                 69 
               
               
                 miR-106a 
                 0.66 
                 0.000811882 
                 0.788 
                 miRNA 
                 135 
               
               
                 miR-379 
                 0.94 
                 0.000829873 
                 0.702 
                 miRNA 
                 57 
               
               
                 miR-570 
                 −0.61 
                 0.000842848 
                 0.697 
                 miRNA 
                 88 
               
               
                 ACA3-2 
                 0.46 
                 0.000855145 
                 0.720 
                 HAcaBox 
                 135 
               
               
                 U55_x 
                 0.50 
                 0.000899835 
                 0.746 
                 CDBox 
                 135 
               
               
                 ACA7_s 
                 −0.34 
                 0.000995798 
                 0.703 
                 HAcaBox 
                 133 
               
               
                 miR-1228 
                 −0.67 
                 0.001047707 
                 0.730 
                 miRNA 
                 114 
               
               
                 miR-199a-3p 
                 0.75 
                 0.001282519 
                 0.729 
                 miRNA 
                 134 
               
               
                 ENSG00000212508 
                 −0.59 
                 0.001303596 
                 0.687 
                 snoRNA 
                 122 
               
               
                 ENSG00000200961 
                 −0.41 
                 0.001335597 
                 0.699 
                 snoRNA 
                 41 
               
               
                 miR-23b 
                 0.45 
                 0.00142683 
                 0.817 
                 miRNA 
                 137 
               
               
                 ENSG00000207177 
                 −0.34 
                 0.001464718 
                 0.697 
                 snoRNA 
                 59 
               
               
                 ACA50 
                 0.41 
                 0.001642667 
                 0.674 
                 HAcaBox 
                 88 
               
               
                 miR-550-star 
                 0.75 
                 0.001687411 
                 0.692 
                 miRNA 
                 58 
               
               
                 miR-617 
                 −0.56 
                 0.001827038 
                 0.676 
                 miRNA 
                 64 
               
               
                 U75 
                 −0.57 
                 0.001850734 
                 0.729 
                 CDBox 
                 134 
               
               
                 HBII-85-2_x 
                 −0.41 
                 0.001956673 
                 0.706 
                 CDBox 
                 135 
               
               
                 miR-508-5p 
                 0.77 
                 0.001978936 
                 0.711 
                 miRNA 
                 92 
               
               
                 miR-200a 
                 0.66 
                 0.002095909 
                 0.676 
                 miRNA 
                 33 
               
               
                 miR-574-5p 
                 0.79 
                 0.002104165 
                 0.749 
                 miRNA 
                 103 
               
               
                 ACA49 
                 −0.37 
                 0.00221029 
                 0.692 
                 HAcaBox 
                 127 
               
               
                 miR-1826 
                 0.46 
                 0.002224183 
                 0.799 
                 miRNA 
                 137 
               
               
                 ENSG00000212377 
                 −0.45 
                 0.002249473 
                 0.676 
                 snoRNA 
                 31 
               
               
                 U45A 
                 −0.44 
                 0.002283826 
                 0.686 
                 CDBox 
                 74 
               
               
                 miR-34a-star 
                 0.61 
                 0.002356105 
                 0.682 
                 miRNA 
                 39 
               
               
                 miR-378-star 
                 0.71 
                 0.002495647 
                 0.710 
                 miRNA 
                 82 
               
               
                 miR-509-5p 
                 0.98 
                 0.002612774 
                 0.705 
                 miRNA 
                 81 
               
               
                 miR-34a 
                 0.79 
                 0.00270219 
                 0.676 
                 miRNA 
                 132 
               
               
                 ACA2a 
                 0.41 
                 0.002741773 
                 0.690 
                 HAcaBox 
                 55 
               
               
                 miR-98 
                 0.63 
                 0.002795533 
                 0.698 
                 miRNA 
                 47 
               
               
                 miR-337-3p 
                 −0.59 
                 0.002924904 
                 0.709 
                 miRNA 
                 94 
               
               
                 U58A 
                 −0.37 
                 0.002957231 
                 0.667 
                 CDBox 
                 129 
               
               
                 miR-30e 
                 0.66 
                 0.003008812 
                 0.673 
                 miRNA 
                 57 
               
               
                 miR-196a 
                 0.99 
                 0.003061353 
                 0.686 
                 miRNA 
                 77 
               
               
                 miR-17-star 
                 0.87 
                 0.003088295 
                 0.675 
                 miRNA 
                 56 
               
               
                 HBII-429 
                 −0.26 
                 0.003238494 
                 0.681 
                 CDBox 
                 137 
               
               
                 HBII-85-5_x 
                 0.38 
                 0.003305314 
                 0.678 
                 CDBox 
                 31 
               
               
                 miR-191-star 
                 −0.50 
                 0.003313493 
                 0.701 
                 miRNA 
                 54 
               
               
                 U102 
                 −0.32 
                 0.003411715 
                 0.662 
                 CDBox 
                 124 
               
               
                 ACA57 
                 −0.35 
                 0.003594842 
                 0.737 
                 scaRna 
                 135 
               
               
                 HBII-234_x 
                 −0.33 
                 0.003636016 
                 0.676 
                 CDBox 
                 102 
               
               
                 hsa-let-7i 
                 0.62 
                 0.003881627 
                 0.748 
                 miRNA 
                 135 
               
               
                 spike_in-control-23 
                 −0.30 
                 0.003884651 
                 0.673 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 HBII-276 
                 0.37 
                 0.003895578 
                 0.676 
                 CDBox 
                 132 
               
               
                 mgU6-53 
                 −0.37 
                 0.004017934 
                 0.667 
                 CDBox 
                 94 
               
               
                 miR-575 
                 −0.55 
                 0.004110263 
                 0.666 
                 miRNA 
                 41 
               
               
                 miR-486-5p 
                 1.06 
                 0.004302169 
                 0.672 
                 miRNA 
                 65 
               
               
                 HBII-85-20_x 
                 0.37 
                 0.004413582 
                 0.662 
                 CDBox 
                 42 
               
               
                 U43 
                 0.49 
                 0.004416752 
                 0.847 
                 CDBox 
                 135 
               
               
                 miR-199b-3p 
                 0.64 
                 0.00460804 
                 0.711 
                 miRNA 
                 134 
               
               
                 U50B 
                 −0.47 
                 0.004646908 
                 0.678 
                 CDBox 
                 135 
               
               
                 HBII-202 
                 −0.37 
                 0.004720422 
                 0.711 
                 CDBox 
                 135 
               
               
                 U32A_x 
                 0.30 
                 0.004742469 
                 0.634 
                 CDBox 
                 136 
               
               
                 U28_x 
                 0.43 
                 0.005077583 
                 0.733 
                 CDBox 
                 133 
               
               
                 miR-223 
                 0.54 
                 0.005512872 
                 0.635 
                 miRNA 
                 83 
               
               
                 U36A 
                 0.26 
                 0.005677159 
                 0.654 
                 CDBox 
                 132 
               
               
                 miR-548a-3p 
                 −0.65 
                 0.005852929 
                 0.669 
                 miRNA 
                 65 
               
               
                 U82 
                 −0.43 
                 0.005931718 
                 0.686 
                 CDBox 
                 131 
               
               
                 ACA13 
                 −0.40 
                 0.005934282 
                 0.697 
                 HAcaBox 
                 134 
               
               
                 ENSG00000208308_x 
                 0.48 
                 0.005982879 
                 0.755 
                 snoRNA 
                 129 
               
               
                 14ql-8 
                 −0.41 
                 0.006020932 
                 0.647 
                 CDBox 
                 61 
               
               
                 ACA41_x 
                 0.38 
                 0.006458897 
                 0.687 
                 HAcaBox 
                 122 
               
               
                 U20 
                 0.34 
                 0.006504359 
                 0.665 
                 CDBox 
                 117 
               
               
                 HBII-210 
                 −0.33 
                 0.006967845 
                 0.684 
                 CDBox 
                 135 
               
               
                 ACA53 
                 0.33 
                 0.007009476 
                 0.656 
                 HAcaBox 
                 105 
               
               
                 miR-188-5p 
                 0.59 
                 0.00730559 
                 0.668 
                 miRNA 
                 51 
               
               
                 miR-653 
                 −0.48 
                 0.007396776 
                 0.652 
                 miRNA 
                 50 
               
               
                 ACA18_x 
                 0.33 
                 0.007506763 
                 0.656 
                 HAcaBox 
                 135 
               
               
                 ENSG00000202440_x 
                 −0.29 
                 0.007646916 
                 0.654 
                 snoRNA 
                 91 
               
               
                 HBII-180C_x 
                 0.33 
                 0.007676115 
                 0.666 
                 CDBox 
                 131 
               
               
                 ENSG00000200235_x 
                 −0.29 
                 0.007854884 
                 0.650 
                 snoRNA 
                 59 
               
               
                 HBII-85-17_x 
                 0.43 
                 0.008991412 
                 0.650 
                 CDBox 
                 57 
               
               
                 miR-205 
                 0.39 
                 0.009048012 
                 0.665 
                 miRNA 
                 135 
               
               
                 ACA27_x 
                 0.24 
                 0.009084132 
                 0.676 
                 HAcaBox 
                 130 
               
               
                 ACA52 
                 0.31 
                 0.009338308 
                 0.664 
                 HAcaBox 
                 121 
               
               
                 U8_x 
                 0.37 
                 0.00950871 
                 0.638 
                 CDBox 
                 135 
               
               
                 ENSG00000201229 
                 0.35 
                 0.009710558 
                 0.662 
                 snoRNA 
                 32 
               
               
                 miR-423-5p 
                 −0.49 
                 0.009884603 
                 0.704 
                 miRNA 
                 132 
               
               
                 U72_x 
                 0.27 
                 0.009902439 
                 0.642 
                 HAcaBox 
                 103 
               
               
                 U50 
                 0.37 
                 0.010130893 
                 0.679 
                 CDBox 
                 134 
               
               
                 miR-34b 
                 0.95 
                 0.010634429 
                 0.647 
                 miRNA 
                 32 
               
               
                 U60 
                 0.33 
                 0.010937468 
                 0.653 
                 CDBox 
                 103 
               
               
                 ACA26 
                 −0.38 
                 0.011152582 
                 0.688 
                 scaRna 
                 125 
               
               
                 ACA21 
                 −0.44 
                 0.011353671 
                 0.661 
                 HAcaBox 
                 133 
               
               
                 U47 
                 0.30 
                 0.01143234 
                 0.651 
                 CDBox 
                 74 
               
               
                 miR-26a 
                 0.40 
                 0.011704306 
                 0.793 
                 miRNA 
                 136 
               
               
                 miR-21-star 
                 0.54 
                 0.012900809 
                 0.648 
                 miRNA 
                 64 
               
               
                 U43_x 
                 0.42 
                 0.013355042 
                 0.808 
                 CDBox 
                 135 
               
               
                 HBII-55 
                 0.27 
                 0.013604568 
                 0.636 
                 CDBox 
                 135 
               
               
                 ACA19 
                 −0.29 
                 0.013694584 
                 0.634 
                 HAcaBox 
                 118 
               
               
                 ENSG00000200897_x 
                 −0.34 
                 0.013964134 
                 0.628 
                 snoRNA 
                 70 
               
               
                 ENSG00000207118 
                 0.42 
                 0.01420542 
                 0.652 
                 snoRNA 
                 93 
               
               
                 U31 
                 −0.42 
                 0.014421405 
                 0.684 
                 CDBox 
                 132 
               
               
                 miR-204 
                 0.70 
                 0.015040931 
                 0.620 
                 miRNA 
                 53 
               
               
                 miR-195 
                 0.59 
                 0.01545596 
                 0.646 
                 miRNA 
                 132 
               
               
                 miR-432 
                 0.70 
                 0.015655418 
                 0.649 
                 miRNA 
                 51 
               
               
                 hsa-let-7c 
                 0.48 
                 0.015958151 
                 0.801 
                 miRNA 
                 135 
               
               
                 ACA10_s 
                 0.33 
                 0.015973764 
                 0.658 
                 HAcaBox 
                 113 
               
               
                 miR-1825 
                 −0.60 
                 0.016002367 
                 0.679 
                 miRNA 
                 132 
               
               
                 14qll-1 
                 −0.53 
                 0.016171959 
                 0.621 
                 CDBox 
                 128 
               
               
                 14qll-21_x 
                 0.32 
                 0.017134601 
                 0.670 
                 CDBox 
                 51 
               
               
                 U34 
                 0.24 
                 0.017406393 
                 0.609 
                 CDBox 
                 137 
               
               
                 miR-138-1-star 
                 0.64 
                 0.017790103 
                 0.611 
                 miRNA 
                 91 
               
               
                 miR-1257 
                 −0.40 
                 0.018052873 
                 0.633 
                 miRNA 
                 31 
               
               
                 ACA51_x 
                 −0.23 
                 0.018226343 
                 0.656 
                 HAcaBox 
                 135 
               
               
                 14qll-1_x 
                 −0.50 
                 0.018262482 
                 0.626 
                 CDBox 
                 131 
               
               
                 ACA43 
                 0.31 
                 0.018272258 
                 0.620 
                 HAcaBox 
                 114 
               
               
                 hsa-let-7d 
                 0.38 
                 0.01921824 
                 0.855 
                 miRNA 
                 135 
               
               
                 14qll-12_x 
                 0.40 
                 0.020581734 
                 0.652 
                 CDBox 
                 85 
               
               
                 14qll-28_x 
                 0.34 
                 0.02070105 
                 0.641 
                 CDBox 
                 51 
               
               
                 spike_in-control-30 
                 −0.36 
                 0.02072199 
                 0.657 
                 Oligonucleotide 
                 53 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 U49B_s 
                 −0.38 
                 0.021350114 
                 0.648 
                 CDBox 
                 129 
               
               
                 U45B_x 
                 −0.32 
                 0.022203975 
                 0.615 
                 CDBox 
                 46 
               
               
                 miR-125b 
                 0.42 
                 0.022489574 
                 0.718 
                 miRNA 
                 135 
               
               
                 ENSG00000202252 
                 −0.23 
                 0.023928736 
                 0.654 
                 snoRNA 
                 135 
               
               
                 miR-1324 
                 −0.37 
                 0.024126574 
                 0.638 
                 miRNA 
                 57 
               
               
                 HBI-61 
                 −0.34 
                 0.024172419 
                 0.639 
                 HAcaBox 
                 134 
               
               
                 miR-99b-star 
                 0.34 
                 0.024194238 
                 0.626 
                 miRNA 
                 74 
               
               
                 miR-20a 
                 0.51 
                 0.024441924 
                 0.640 
                 miRNA 
                 134 
               
               
                 U54 
                 0.36 
                 0.02454759 
                 0.749 
                 CDBox 
                 135 
               
               
                 ENSG00000200932 
                 −0.31 
                 0.024839208 
                 0.656 
                 snoRNA 
                 47 
               
               
                 ENSG00000212326 
                 0.34 
                 0.024965859 
                 0.611 
                 snoRNA 
                 104 
               
               
                 U95 
                 −0.35 
                 0.026080784 
                 0.723 
                 CDBox 
                 135 
               
               
                 miR-193b-star 
                 0.57 
                 0.028801534 
                 0.684 
                 miRNA 
                 77 
               
               
                 ACA5_x 
                 0.29 
                 0.0293861 
                 0.662 
                 HAcaBox 
                 103 
               
               
                 ACA1_x 
                 −0.30 
                 0.029505966 
                 0.627 
                 HAcaBox 
                 51 
               
               
                 mgU6-53_x 
                 −0.26 
                 0.03076806 
                 0.618 
                 CDBox 
                 113 
               
               
                 U26 
                 −0.36 
                 0.030778322 
                 0.608 
                 CDBox 
                 135 
               
               
                 miR-24 
                 0.36 
                 0.031162316 
                 0.771 
                 miRNA 
                 135 
               
               
                 spike_in-control-29 
                 −0.24 
                 0.03150161 
                 0.615 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 miR-19b 
                 0.57 
                 0.03221182 
                 0.612 
                 miRNA 
                 124 
               
               
                 miR-513c 
                 0.78 
                 0.032937767 
                 0.632 
                 miRNA 
                 73 
               
               
                 U42B_x 
                 0.27 
                 0.033688384 
                 0.613 
                 CDBox 
                 109 
               
               
                 miR-20a-star 
                 −0.90 
                 0.033742183 
                 0.639 
                 miRNA 
                 61 
               
               
                 U25 
                 −0.30 
                 0.035318167 
                 0.655 
                 CDBox 
                 135 
               
               
                 miR-455-3p 
                 0.34 
                 0.036186611 
                 0.643 
                 miRNA 
                 136 
               
               
                 U109 
                 0.32 
                 0.036189693 
                 0.634 
                 scaRna 
                 69 
               
               
                 miR-938 
                 −0.32 
                 0.037572276 
                 0.639 
                 miRNA 
                 45 
               
               
                 ENSG00000207503 
                 −0.27 
                 0.037791965 
                 0.622 
                 snoRNA 
                 42 
               
               
                 HBII-289 
                 −0.24 
                 0.041306388 
                 0.652 
                 CDBox 
                 135 
               
               
                 U17b_x 
                 0.34 
                 0.041402099 
                 0.728 
                 HAcaBox 
                 135 
               
               
                 ACA8_x 
                 −0.28 
                 0.042136422 
                 0.624 
                 HAcaBox 
                 125 
               
               
                 ACA44 
                 0.29 
                 0.043557966 
                 0.693 
                 HAcaBox 
                 135 
               
               
                 U45C_x 
                 −0.33 
                 0.043838427 
                 0.586 
                 CDBox 
                 72 
               
               
                 ENSG00000201133 
                 −0.27 
                 0.045035669 
                 0.645 
                 snoRNA 
                 35 
               
               
                 ACA55 
                 −0.26 
                 0.045152107 
                 0.606 
                 HAcaBox 
                 85 
               
               
                 miR-451 
                 0.53 
                 0.046730942 
                 0.628 
                 miRNA 
                 72 
               
               
                 14qll-26 
                 −0.32 
                 0.047726134 
                 0.619 
                 CDBox 
                 32 
               
               
                 miR-141 
                 0.44 
                 0.049534195 
                 0.627 
                 miRNA 
                 69 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 50 miRNAs most significantly expressed between melanoma and nevi 
               
             
          
           
               
                 Probe Name 
                 Log2-FC 
                 P value 
                 AUC 
                 ProbeType 
                 #Sample Detected 
               
               
                   
               
             
          
           
               
                 miR-132 
                 2.98 
                  1.2728E−32 
                 0.994 
                 miRNA 
                 96 
               
               
                 miR-150 
                 3.27 
                 5.83192E−30 
                 1.000 
                 miRNA 
                 117 
               
               
                 miR-339-5p 
                 2.73 
                 1.69388E−27 
                 0.988 
                 miRNA 
                 75 
               
               
                 miR-15b 
                 3.02 
                 6.41784E−27 
                 0.978 
                 miRNA 
                 125 
               
               
                 miR-342-3p 
                 2.23 
                 7.03798E−26 
                 0.993 
                 miRNA 
                 136 
               
               
                 miR-572 
                 −2.78 
                 1.00008E−25 
                 0.970 
                 miRNA 
                 116 
               
               
                 miR-155 
                 4.14 
                 1.28454E−25 
                 0.981 
                 miRNA 
                 123 
               
               
                 miR-425 
                 2.74 
                 8.13135E−25 
                 0.975 
                 miRNA 
                 114 
               
               
                 miR-1202 
                 −2.63 
                 2.97795E−23 
                 0.986 
                 miRNA 
                 57 
               
               
                 miR-1268 
                 −2.68 
                 6.05905E−23 
                 0.997 
                 miRNA 
                 133 
               
               
                 HBII-382_s 
                 −1.71 
                 6.58931E−22 
                 0.971 
                 scaRna 
                 126 
               
               
                 miR-1225-5p 
                 −2.36 
                 1.05361E−21 
                 0.953 
                 miRNA 
                 90 
               
               
                 miR-30c 
                 2.39 
                 2.58594E−21 
                 0.980 
                 miRNA 
                 125 
               
               
                 miR-106b-star 
                 2.24 
                 3.48507E−21 
                 0.961 
                 miRNA 
                 72 
               
               
                 miR-125a-5p 
                 2.39 
                 6.90609E−21 
                 0.967 
                 miRNA 
                 128 
               
               
                 mgU6-53B 
                 −1.51 
                 1.74782E−20 
                 0.973 
                 CDBox 
                 99 
               
               
                 miR-25 
                 2.57 
                 1.52769E−19 
                 0.956 
                 miRNA 
                 118 
               
               
                 miR-149-star 
                 −2.03 
                 1.64858E−19 
                 1.000 
                 miRNA 
                 135 
               
               
                 miR-939 
                 −2.21 
                  1.9129E−19 
                 0.973 
                 miRNA 
                 57 
               
               
                 miR-92b-star 
                 −2.30 
                 2.05085E−19 
                 0.969 
                 miRNA 
                 111 
               
               
                 miR-500-star 
                 2.38 
                 3.11256E−19 
                 0.956 
                 miRNA 
                 97 
               
               
                 miR-22 
                 2.69 
                  3.8844E−19 
                 0.965 
                 miRNA 
                 120 
               
               
                 HBII-142_x 
                 −1.22 
                 4.93318E−19 
                 0.981 
                 CDBox 
                 135 
               
               
                 miR-181b 
                 2.41 
                 1.00639E−18 
                 0.960 
                 miRNA 
                 130 
               
               
                 HBII-142 
                 −1.33 
                 1.18794E−18 
                 0.988 
                 CDBox 
                 135 
               
               
                 U38B 
                 −1.95 
                 1.35964E−18 
                 0.975 
                 CDBox 
                 134 
               
               
                 miR-663 
                 −2.10 
                 2.04672E−18 
                 0.984 
                 miRNA 
                 134 
               
               
                 miR-1224-5p 
                 −2.54 
                 2.91169E−18 
                 0.946 
                 miRNA 
                 78 
               
               
                 miR-23a 
                 1.18 
                 2.99523E−18 
                 0.967 
                 miRNA 
                 137 
               
               
                 HBII-85-6_x 
                 −1.73 
                 4.78418E−18 
                 0.939 
                 CDBox 
                 137 
               
               
                 miR-1207-5p 
                 −2.14 
                 4.98991E−18 
                 0.995 
                 miRNA 
                 133 
               
               
                 miR-1301 
                 2.36 
                 5.22389E−18 
                 0.931 
                 miRNA 
                 54 
               
               
                 miR-1228-star 
                 −2.41 
                 5.51513E−18 
                 0.997 
                 miRNA 
                 134 
               
               
                 miR-345 
                 2.45 
                 6.43552E−18 
                 0.942 
                 miRNA 
                 75 
               
               
                 miR-30a-star 
                 2.32 
                 6.92165E−18 
                 0.932 
                 miRNA 
                 64 
               
               
                 ENSG00000199411_s 
                 −1.97 
                 7.23325E−18 
                 0.991 
                 snoRNA 
                 135 
               
               
                 ENSG00000202327 
                 −1.40 
                 8.75839E−18 
                 0.938 
                 snoRNA 
                 54 
               
               
                 miR-92a 
                 1.67 
                 9.49427E−18 
                 0.979 
                 miRNA 
                 136 
               
               
                 miR-127-3p 
                 2.43 
                 9.84615E−18 
                 0.936 
                 miRNA 
                 89 
               
               
                 HBII-85-26 
                 −2.07 
                 1.50619E−17 
                 0.951 
                 CDBox 
                 136 
               
               
                 miR-1308 
                 −2.14 
                 1.99888E−17 
                 0.999 
                 miRNA 
                 135 
               
               
                 miR-31 
                 3.29 
                 2.46697E−17 
                 0.913 
                 miRNA 
                 103 
               
               
                 miR-921 
                 −1.46 
                 2.61089E−17 
                 0.928 
                 miRNA 
                 49 
               
               
                 miR-146b-5p 
                 2.37 
                 6.10422E−17 
                 0.918 
                 miRNA 
                 83 
               
               
                 miR-768-3p 
                 −1.05 
                 6.66153E−17 
                 0.949 
                 miRNA 
                 137 
               
               
                 miR-708 
                 2.29 
                 6.86187E−17 
                 0.929 
                 miRNA 
                 102 
               
               
                 miR-139-5p 
                 2.23 
                 2.78962E−16 
                 0.922 
                 miRNA 
                 80 
               
               
                 ACA24_x 
                 1.33 
                 3.37973E−16 
                 0.925 
                 HAcaBox 
                 98 
               
               
                 miR-501-3p 
                 1.83 
                 4.76751E−16 
                 0.923 
                 miRNA 
                 80 
               
               
                 miR-502-3p 
                 2.16 
                 5.19509E−16 
                 0.925 
                 miRNA 
                 110 
               
               
                 miR-923 
                 −1.86 
                 5.55191E−16 
                 0.996 
                 miRNA 
                 135 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 miRNAs significantly expressed between melanoma and normal skin 
               
             
          
           
               
                   
                 Log2- 
                   
                   
                   
                 #Sample 
               
               
                 Probe Name 
                 FC 
                 P value 
                 AUC 
                 ProbeType 
                 Detected 
               
               
                   
               
             
          
           
               
                 miR-146a 
                 4.6 
                 9.43624E−20 
                 0.983 
                 miRNA 
                 129 
               
               
                 miR-509-3p 
                 5.2 
                 5.61558E−17 
                 0.969 
                 miRNA 
                 116 
               
               
                 14qll-14 
                 −2.6 
                 1.16829E−14 
                 0.978 
                 CDBox 
                 56 
               
               
                 miR-25 
                 2.4 
                   2.08E−14 
                 0.949 
                 miRNA 
                 118 
               
               
                 miR-138 
                 3.2 
                 2.51219E−13 
                 0.954 
                 miRNA 
                 82 
               
               
                 miR-509-3-5p 
                 3.6 
                 2.94532E−13 
                 0.963 
                 miRNA 
                 107 
               
               
                 miR-506 
                 3.4 
                 6.99753E−13 
                 0.954 
                 miRNA 
                 76 
               
               
                 14qll-14_x 
                 −2.2 
                 8.36026E−13 
                 0.970 
                 CDBox 
                 56 
               
               
                 14ql-4 
                 −2.1 
                  8.9267E−13 
                 0.964 
                 CDBox 
                 80 
               
               
                 miR-30b 
                 2.2 
                 2.43822E−12 
                 0.957 
                 miRNA 
                 128 
               
               
                 miR-513a-5p 
                 3.5 
                 3.17158E−12 
                 0.939 
                 miRNA 
                 105 
               
               
                 miR-21 
                 3.3 
                 4.58391E−12 
                 0.926 
                 miRNA 
                 79 
               
               
                 Z17B 
                 −1.0 
                 6.92137E−12 
                 0.956 
                 CDBox 
                 117 
               
               
                 U33 
                 −0.9 
                 8.19977E−12 
                 0.968 
                 CDBox 
                 137 
               
               
                 miR-20b 
                 2.8 
                 2.24496E−11 
                 0.939 
                 miRNA 
                 99 
               
               
                 hsa-let-7i 
                 2.3 
                 3.27742E−11 
                 0.973 
                 miRNA 
                 135 
               
               
                 HBII-239 
                 −1.0 
                  3.9295E−11 
                 0.920 
                 CDBox 
                 135 
               
               
                 miR-146b-5p 
                 2.3 
                 8.71488E−11 
                 0.910 
                 miRNA 
                 83 
               
               
                 14qll-26_x 
                 −1.8 
                 9.17329E−11 
                 0.914 
                 CDBox 
                 41 
               
               
                 miR-155 
                 2.8 
                 1.12361E−10 
                 0.940 
                 miRNA 
                 123 
               
               
                 miR-151-3p 
                 1.8 
                  1.7141E−10 
                 0.922 
                 miRNA 
                 117 
               
               
                 HBII-289 
                 −1.1 
                 1.76712E−10 
                 0.963 
                 CDBox 
                 135 
               
               
                 14qll-12_x 
                 −1.7 
                  1.9475E−10 
                 0.907 
                 CDBox 
                 85 
               
               
                 miR-1274a 
                 1.9 
                 3.28343E−10 
                 0.928 
                 miRNA 
                 48 
               
               
                 HBII-180A_x 
                 −0.8 
                 4.68853E−10 
                 0.933 
                 CDBox 
                 123 
               
               
                 miR-1301 
                 2.0 
                 7.22101E−10 
                 0.896 
                 miRNA 
                 54 
               
               
                 14qll-1_x 
                 −1.7 
                 7.72545E−10 
                 0.965 
                 CDBox 
                 131 
               
               
                 miR-193b 
                 −1.4 
                  8.7852E−10 
                 0.974 
                 miRNA 
                 130 
               
               
                 miR-510 
                 2.7 
                 1.03096E−09 
                 0.914 
                 miRNA 
                 82 
               
               
                 miR-126 
                 2.1 
                 1.35297E−09 
                 0.959 
                 miRNA 
                 133 
               
               
                 miR-24-2-star 
                 1.8 
                 1.36986E−09 
                 0.895 
                 miRNA 
                 63 
               
               
                 miR-106b 
                 2.1 
                 1.91606E−09 
                 0.930 
                 miRNA 
                 127 
               
               
                 HBII-276 
                 −1.0 
                 2.06051E−09 
                 0.908 
                 CDBox 
                 132 
               
               
                 miR-532-5p 
                 1.8 
                  2.9654E−09 
                 0.887 
                 miRNA 
                 113 
               
               
                 14qll-12 
                 −1.7 
                 3.71443E−09 
                 0.859 
                 CDBox 
                 58 
               
               
                 miR-19b 
                 2.1 
                 3.71682E−09 
                 0.908 
                 miRNA 
                 124 
               
               
                 miR-30a 
                 1.6 
                 6.61873E−09 
                 0.894 
                 miRNA 
                 113 
               
               
                 HBII-85-26_x 
                 −1.1 
                 7.76845E−09 
                 0.895 
                 CDBox 
                 135 
               
               
                 miR-150 
                 1.7 
                 1.08172E−08 
                 0.894 
                 miRNA 
                 117 
               
               
                 14qll-26 
                 −1.5 
                 1.16704E−08 
                 0.869 
                 CDBox 
                 32 
               
               
                 miR-324-5p 
                 1.6 
                 1.24724E−08 
                 0.880 
                 miRNA 
                 102 
               
               
                 14ql-8 
                 −1.2 
                 1.39067E−08 
                 0.894 
                 CDBox 
                 61 
               
               
                 miR-185 
                 1.6 
                 1.44139E−08 
                 0.952 
                 miRNA 
                 132 
               
               
                 miR-194 
                 1.8 
                 1.72961E−08 
                 0.883 
                 miRNA 
                 54 
               
               
                 14qll-1 
                 −1.5 
                 2.76507E−08 
                 0.928 
                 CDBox 
                 128 
               
               
                 HBII-202 
                 −1.0 
                 2.89826E−08 
                 0.946 
                 CDBox 
                 135 
               
               
                 miR-768-5p 
                 −1.6 
                  3.1365E−08 
                 0.986 
                 miRNA 
                 135 
               
               
                 miR-421 
                 1.6 
                 4.42916E−08 
                 0.872 
                 miRNA 
                 68 
               
               
                 miR-28-5p 
                 1.7 
                 5.45027E−08 
                 0.877 
                 miRNA 
                 105 
               
               
                 miR-151-5p 
                 1.2 
                 5.48542E−08 
                 0.968 
                 miRNA 
                 135 
               
               
                 miR-15a 
                 2.0 
                 5.77662E−08 
                 0.889 
                 miRNA 
                 80 
               
               
                 miR-26a 
                 1.3 
                  6.8712E−08 
                 0.968 
                 miRNA 
                 136 
               
               
                 U25 
                 −1.0 
                 7.35643E−08 
                 0.976 
                 CDBox 
                 135 
               
               
                 HBII-180C 
                 −0.9 
                 7.47464E−08 
                 0.836 
                 CDBox 
                 115 
               
               
                 miR-584 
                 1.9 
                  8.0812E−08 
                 0.863 
                 miRNA 
                 57 
               
               
                 hsa-let-7f 
                 2.2 
                 1.05873E−07 
                 0.924 
                 miRNA 
                 130 
               
               
                 miR-1268 
                 −1.7 
                 1.09503E−07 
                 0.954 
                 miRNA 
                 133 
               
               
                 miR-572 
                 −1.4 
                 1.12523E−07 
                 0.886 
                 miRNA 
                 116 
               
               
                 ENSG00000200897 
                 −0.9 
                 1.38587E−07 
                 0.843 
                 snoRNA 
                 38 
               
               
                 miR-508-5p 
                 1.8 
                 1.57345E−07 
                 0.873 
                 miRNA 
                 92 
               
               
                 hsa-let-7g 
                 1.8 
                 1.57818E−07 
                 0.916 
                 miRNA 
                 127 
               
               
                 miR-20a 
                 1.7 
                 1.58351E−07 
                 0.926 
                 miRNA 
                 134 
               
               
                 miR-1225-5p 
                 −1.3 
                  6.2291E−07 
                 0.890 
                 miRNA 
                 90 
               
               
                 U55 
                 −0.8 
                  1.8721E−07 
                 0.942 
                 CDBox 
                 135 
               
               
                 miR-509-5p 
                 2.3 
                 1.95354E−07 
                 0.864 
                 miRNA 
                 81 
               
               
                 HBII-85-6_x 
                 −1.1 
                  2.0107E−07 
                 0.872 
                 CDBox 
                 137 
               
               
                 U99 
                 −0.7 
                 2.22915E−07 
                 0.872 
                 HAcaBox 
                 137 
               
               
                 miR-501-3p 
                 1.4 
                 2.27557E−07 
                 0.858 
                 miRNA 
                 80 
               
               
                 miR-29a 
                 1.8 
                 2.42514E−07 
                 0.907 
                 miRNA 
                 128 
               
               
                 miR-1274b 
                 1.6 
                  2.6096E−07 
                 0.863 
                 miRNA 
                 112 
               
               
                 miR-210 
                 −1.2 
                 2.81748E−07 
                 0.895 
                 miRNA 
                 130 
               
               
                 HBII-85-26 
                 −1.4 
                  3.5949E−07 
                 0.896 
                 CDBox 
                 136 
               
               
                 miR-199a-5p 
                 −0.8 
                 3.93185E−07 
                 0.870 
                 miRNA 
                 129 
               
               
                 miR-149 
                 −1.6 
                 5.63545E−07 
                 0.920 
                 miRNA 
                 100 
               
               
                 miR-1307 
                 −0.9 
                  5.6459E−07 
                 0.907 
                 miRNA 
                 97 
               
               
                 miR-744 
                 −1.1 
                 5.78243E−07 
                 0.971 
                 miRNA 
                 133 
               
               
                 miR-92b-star 
                 −1.4 
                 6.45948E−07 
                 0.907 
                 miRNA 
                 111 
               
               
                 ACA11 
                 0.8 
                 7.08497E−07 
                 0.842 
                 HAcaBox 
                 48 
               
               
                 miR-27a 
                 1.0 
                 7.17204E−07 
                 0.887 
                 miRNA 
                 136 
               
               
                 miR-34a-star 
                 1.4 
                 8.89848E−07 
                 0.842 
                 miRNA 
                 39 
               
               
                 U46 
                 −0.8 
                  9.2248E−07 
                 0.884 
                 CDBox 
                 133 
               
               
                 miR-214 
                 −1.2 
                 1.02234E−06 
                 0.930 
                 miRNA 
                 135 
               
               
                 miR-132 
                 1.2 
                 1.15319E−06 
                 0.808 
                 miRNA 
                 96 
               
               
                 miR-18a 
                 2.0 
                  1.3554E−06 
                 0.866 
                 miRNA 
                 77 
               
               
                 miR-125a-5p 
                 −1.0 
                 1.86884E−06 
                 0.934 
                 miRNA 
                 128 
               
               
                 14ql-8_x 
                 −0.9 
                 1.95428E−06 
                 0.831 
                 CDBox 
                 74 
               
               
                 miR-663 
                 −1.3 
                  1.9913E−06 
                 0.910 
                 miRNA 
                 134 
               
               
                 miR-16 
                 1.1 
                 2.78721E−06 
                 0.955 
                 miRNA 
                 135 
               
               
                 HBII-142_x 
                 −0.8 
                 2.95334E−06 
                 0.909 
                 CDBox 
                 135 
               
               
                 U103_s 
                 −0.8 
                  3.6466E−06 
                 0.825 
                 CDBox 
                 111 
               
               
                 U55_x 
                 −0.8 
                 3.84586E−06 
                 0.958 
                 CDBox 
                 135 
               
               
                 HBII-142 
                 −0.8 
                 3.88875E−06 
                 0.917 
                 CDBox 
                 135 
               
               
                 miR-130b 
                 1.9 
                 4.19812E−06 
                 0.858 
                 miRNA 
                 90 
               
               
                 miR-339-3p 
                 1.1 
                 4.25751E−06 
                 0.796 
                 miRNA 
                 60 
               
               
                 miR-30d 
                 1.2 
                 4.40714E−06 
                 0.902 
                 miRNA 
                 131 
               
               
                 miR-196a 
                 2.0 
                 4.53608E−06 
                 0.833 
                 miRNA 
                 77 
               
               
                 miR-199a-3p 
                 1.4 
                 5.28457E−06 
                 0.889 
                 miRNA 
                 134 
               
               
                 miR-211 
                 2.1 
                  5.7974E−06 
                 0.859 
                 miRNA 
                 84 
               
               
                 miR-30e 
                 1.2 
                 5.88414E−06 
                 0.839 
                 miRNA 
                 57 
               
               
                 ACA54 
                 −0.6 
                 7.09713E−06 
                 0.861 
                 HAcaBox 
                 133 
               
               
                 miR-106b-star 
                 1.1 
                 7.86756E−06 
                 0.720 
                 miRNA 
                 72 
               
               
                 14qll-3 
                 −1.1 
                 8.39354E−06 
                 0.832 
                 CDBox 
                 95 
               
               
                 HBII-55 
                 −0.6 
                 8.41728E−06 
                 0.860 
                 CDBox 
                 135 
               
               
                 miR-486-5p 
                 −2.2 
                 1.02088E−05 
                 0.829 
                 miRNA 
                 65 
               
               
                 miR-145 
                 −1.3 
                 1.03604E−05 
                 0.831 
                 miRNA 
                 135 
               
               
                 miR-34a 
                 1.4 
                 1.04763E−05 
                 0.868 
                 miRNA 
                 132 
               
               
                 miR-106a 
                 1.2 
                 1.06568E−05 
                 0.919 
                 miRNA 
                 135 
               
               
                 miR-1271 
                 1.3 
                 1.06608E−05 
                 0.829 
                 miRNA 
                 70 
               
               
                 miR-500 
                 1.3 
                 1.07278E−05 
                 0.816 
                 miRNA 
                 86 
               
               
                 miR-625 
                 1.4 
                 1.08708E−05 
                 0.800 
                 miRNA 
                 59 
               
               
                 14qll-9_x 
                 −0.8 
                 1.14849E−05 
                 0.821 
                 CDBox 
                 36 
               
               
                 14qll-21_x 
                 −0.9 
                 1.17647E−05 
                 0.797 
                 CDBox 
                 51 
               
               
                 U83 
                 −0.6 
                 1.19481E−05 
                 0.889 
                 CDBox 
                 135 
               
               
                 miR-638 
                 −1.3 
                 1.34963E−05 
                 0.914 
                 miRNA 
                 135 
               
               
                 HBII-180C_x 
                 −0.6 
                 1.39632E−05 
                 0.841 
                 CDBox 
                 131 
               
               
                 ENSG00000212139_x 
                 −0.6 
                 1.67825E−05 
                 0.831 
                 snoRNA 
                 124 
               
               
                 miR-628-3p 
                 1.4 
                 1.76296E−05 
                 0.811 
                 miRNA 
                 38 
               
               
                 U104 
                 −0.8 
                 1.84466E−05 
                 0.886 
                 CDBox 
                 135 
               
               
                 miR-10b 
                 1.2 
                 2.02886E−05 
                 0.829 
                 miRNA 
                 106 
               
               
                 U34 
                 −0.4 
                 2.06445E−05 
                 0.807 
                 CDBox 
                 137 
               
               
                 miR-127-3p 
                 −1.1 
                 2.16929E−05 
                 0.799 
                 miRNA 
                 89 
               
               
                 14qll-28_x 
                 −0.9 
                 2.21554E−05 
                 0.815 
                 CDBox 
                 51 
               
               
                 miR-1207-5p 
                 −1.2 
                 2.25272E−05 
                 0.917 
                 miRNA 
                 133 
               
               
                 miR-199b-3p 
                 1.3 
                 2.29577E−05 
                 0.863 
                 miRNA 
                 134 
               
               
                 U35A 
                 −0.7 
                 2.50745E−05 
                 0.900 
                 CDBox 
                 135 
               
               
                 U91_s 
                 −0.8 
                 2.92768E−05 
                 0.806 
                 scaRna 
                 132 
               
               
                 spike_in-control-36 
                 0.6 
                 2.95775E−05 
                 0.865 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 U32A_x 
                 −0.4 
                 3.05381E−05 
                 0.827 
                 CDBox 
                 136 
               
               
                 miR-21-star 
                 1.2 
                 3.24139E−05 
                 0.808 
                 miRNA 
                 64 
               
               
                 miR-149-star 
                 −1.1 
                 4.06943E−05 
                 0.888 
                 miRNA 
                 135 
               
               
                 miR-193b-star 
                 −1.4 
                 4.09065E−05 
                 0.831 
                 miRNA 
                 77 
               
               
                 U67_x 
                 0.8 
                 4.09255E−05 
                 0.792 
                 HAcaBox 
                 50 
               
               
                 ACA20 
                 −0.8 
                 4.42355E−05 
                 0.839 
                 HAcaBox 
                 131 
               
               
                 miR-1287 
                 1.2 
                 4.69003E−05 
                 0.775 
                 miRNA 
                 40 
               
               
                 ACA64 
                 −0.7 
                 4.84198E−05 
                 0.802 
                 HAcaBox 
                 33 
               
               
                 U57 
                 −0.8 
                 5.04018E−05 
                 0.944 
                 CDBox 
                 135 
               
               
                 miR-193a-3p 
                 1.1 
                 5.08442E−05 
                 0.766 
                 miRNA 
                 42 
               
               
                 miR-371-5p 
                 1.1 
                 5.19682E−05 
                 0.775 
                 miRNA 
                 64 
               
               
                 HBII-85-8_x 
                 −0.7 
                 5.46471E−05 
                 0.782 
                 CDBox 
                 135 
               
               
                 miR-503 
                 1.4 
                  5.4991E−05 
                 0.794 
                 miRNA 
                 41 
               
               
                 miR-138-1-star 
                 1.5 
                 5.60721E−05 
                 0.877 
                 miRNA 
                 91 
               
               
                 ACA67_x 
                 0.7 
                 5.78125E−05 
                 0.790 
                 HAcaBox 
                 54 
               
               
                 miR-1228-star 
                 −1.3 
                 5.81565E−05 
                 0.895 
                 miRNA 
                 134 
               
               
                 U41 
                 −0.8 
                 5.85491E−05 
                 0.875 
                 CDBox 
                 135 
               
               
                 miR-193a-5p 
                 −1.2 
                 5.98826E−05 
                 0.897 
                 miRNA 
                 121 
               
               
                 ENSG00000202252 
                 −0.5 
                 6.64141E−05 
                 0.839 
                 snoRNA 
                 135 
               
               
                 14ql-4_x 
                 −1.0 
                  6.7457E−05 
                 0.760 
                 CDBox 
                 51 
               
               
                 miR-508-3p 
                 1.7 
                 6.91432E−05 
                 0.801 
                 miRNA 
                 74 
               
               
                 miR-200b 
                 1.4 
                 7.07093E−05 
                 0.779 
                 miRNA 
                 109 
               
               
                 miR-660 
                 1.2 
                 7.13979E−05 
                 0.791 
                 miRNA 
                 71 
               
               
                 U67 
                 0.8 
                 7.16134E−05 
                 0.774 
                 HAcaBox 
                 44 
               
               
                 U50B 
                 −0.8 
                  7.249E−05 
                 0.869 
                 CDBox 
                 135 
               
               
                 miR-513c 
                 1.9 
                 7.46404E−05 
                 0.771 
                 miRNA 
                 73 
               
               
                 14ql-9_x 
                 −0.7 
                 7.67649E−05 
                 0.772 
                 CDBox 
                 34 
               
               
                 U60 
                 −0.6 
                 8.34902E−05 
                 0.779 
                 CDBox 
                 103 
               
               
                 U102 
                 0.5 
                 8.47602E−05 
                 0.772 
                 CDBox 
                 124 
               
               
                 EN8G00000200235_x 
                 −0.5 
                  9.4306E−05 
                 0.793 
                 snoRNA 
                 59 
               
               
                 miR-17 
                 1.0 
                 9.79941E−05 
                 0.910 
                 miRNA 
                 134 
               
               
                 U36C 
                 −0.6 
                 0.000107866 
                 0.854 
                 CDBox 
                 135 
               
               
                 miR-491-5p 
                 0.9 
                 0.000121664 
                 0.753 
                 miRNA 
                 92 
               
               
                 miR-425 
                 1.0 
                 0.000138461 
                 0.771 
                 miRNA 
                 114 
               
               
                 miR-500-star 
                 1.0 
                 0.000148221 
                 0.761 
                 miRNA 
                 97 
               
               
                 U78_x 
                 1.0 
                 0.000150233 
                 0.872 
                 CDBox 
                 134 
               
               
                 ENSG00000202327 
                 −0.6 
                 0.000153478 
                 0.783 
                 snoRNA 
                 54 
               
               
                 miR-181c 
                 1.1 
                 0.000156102 
                 0.772 
                 miRNA 
                 59 
               
               
                 ENSG00000212523_x 
                 −0.8 
                 0.000165203 
                 0.772 
                 snoRNA 
                 135 
               
               
                 mgU6-53B_x 
                 −0.5 
                 0.00016649 
                 0.784 
                 CDBox 
                 121 
               
               
                 miR-99b-star 
                 0.8 
                 0.00017311 
                 0.743 
                 miRNA 
                 74 
               
               
                 U53 
                 −0.7 
                 0.000175804 
                 0.763 
                 CDBox 
                 126 
               
               
                 miR-296-3p 
                 −1.0 
                 0.000180884 
                 0.771 
                 miRNA 
                 90 
               
               
                 U50B_x 
                 −0.7 
                 0.000181435 
                 0.829 
                 CDBox 
                 135 
               
               
                 miR-30a-star 
                 1.2 
                 0.00018331 
                 0.766 
                 miRNA 
                 64 
               
               
                 U46_x 
                 −0.8 
                 0.000183742 
                 0.819 
                 CDBox 
                 131 
               
               
                 ENSG00000207410_x 
                 −0.5 
                 0.000198904 
                 0.779 
                 snoRNA 
                 55 
               
               
                 miR-99a 
                 −0.8 
                 0.000204711 
                 0.829 
                 miRNA 
                 135 
               
               
                 miR-513b 
                 1.4 
                 0.000214575 
                 0.781 
                 miRNA 
                 64 
               
               
                 miR-23a 
                 0.6 
                 0.000218135 
                 0.908 
                 miRNA 
                 137 
               
               
                 miR-31 
                 1.7 
                 0.00023269 
                 0.721 
                 miRNA 
                 103 
               
               
                 miR-345 
                 1.2 
                 0.000250483 
                 0.782 
                 miRNA 
                 75 
               
               
                 miR-212 
                 1.5 
                 0.000254534 
                 0.831 
                 miRNA 
                 62 
               
               
                 ENSG00000212266 
                 −0.6 
                 0.000255339 
                 0.777 
                 snoRNA 
                 93 
               
               
                 miR-152 
                 1.1 
                 0.000260732 
                 0.813 
                 miRNA 
                 122 
               
               
                 U19 
                 0.6 
                 0.000277432 
                 0.757 
                 HAcaBox 
                 118 
               
               
                 miR-886-3p 
                 1.0 
                 0.000298839 
                 0.768 
                 miRNA 
                 110 
               
               
                 U73a 
                 −0.6 
                 0.000338569 
                 0.846 
                 CDBox 
                 135 
               
               
                 miR-423-3p 
                 −0.7 
                 0.000347496 
                 0.848 
                 miRNA 
                 107 
               
               
                 mgh28S-2411 
                 −0.6 
                 0.00034785 
                 0.901 
                 CDBox 
                 135 
               
               
                 miR-103 
                 0.8 
                 0.000430411 
                 0.930 
                 miRNA 
                 135 
               
               
                 miR-629 
                 1.1 
                 0.000473215 
                 0.769 
                 miRNA 
                 64 
               
               
                 U65 
                 −0.6 
                 0.000562655 
                 0.778 
                 HAcaBox 
                 128 
               
               
                 14ql-7 
                 −0.7 
                 0.000569692 
                 0.803 
                 CDBox 
                 51 
               
               
                 miR-933 
                 −0.6 
                 0.000572446 
                 0.769 
                 miRNA 
                 100 
               
               
                 miR-205 
                 −0.6 
                 0.000653671 
                 0.767 
                 miRNA 
                 135 
               
               
                 U105 
                 −0.4 
                 0.000692027 
                 0.749 
                 CDBox 
                 126 
               
               
                 miR-378-star 
                 1.1 
                 0.000731339 
                 0.769 
                 miRNA 
                 82 
               
               
                 miR-939 
                 −0.9 
                 0.000748523 
                 0.822 
                 miRNA 
                 57 
               
               
                 hsa-let-7d 
                 0.8 
                 0.000759497 
                 0.978 
                 miRNA 
                 135 
               
               
                 miR-362-5p 
                 1.2 
                 0.000775756 
                 0.762 
                 miRNA 
                 94 
               
               
                 ACA9 
                 0.7 
                 0.000834583 
                 0.739 
                 HAcaBox 
                 82 
               
               
                 miR-10a 
                 1.1 
                 0.000844868 
                 0.759 
                 miRNA 
                 69 
               
               
                 miR-877 
                 −0.9 
                 0.000845569 
                 0.789 
                 miRNA 
                 126 
               
               
                 miR-128 
                 1.1 
                 0.000865867 
                 0.762 
                 miRNA 
                 44 
               
               
                 miR-27b 
                 0.8 
                 0.000876954 
                 0.799 
                 miRNA 
                 133 
               
               
                 miR-17-star 
                 1.2 
                 0.000905238 
                 0.748 
                 miRNA 
                 56 
               
               
                 mgh18S-121 
                 −0.5 
                 0.000964406 
                 0.789 
                 CDBox 
                 134 
               
               
                 ENSG00000200394 
                 0.5 
                 0.000968073 
                 0.772 
                 snoRNA 
                 119 
               
               
                 ENSG00000212315 
                 0.6 
                 0.000971189 
                 0.757 
                 snoRNA 
                 117 
               
               
                 ENSG00000212615_x 
                 −0.5 
                 0.000998331 
                 0.707 
                 snoRNA 
                 102 
               
               
                 miR-148a 
                 0.9 
                 0.001014157 
                 0.733 
                 miRNA 
                 41 
               
               
                 miR-923 
                 −0.9 
                 0.001032772 
                 0.826 
                 miRNA 
                 135 
               
               
                 ACA20_x 
                 −0.6 
                 0.001087206 
                 0.830 
                 HAcaBox 
                 134 
               
               
                 ENSG00000212579_x 
                 −0.6 
                 0.001126846 
                 0.765 
                 snoRNA 
                 100 
               
               
                 miR-589-star 
                 0.5 
                 0.001225281 
                 0.703 
                 miRNA 
                 43 
               
               
                 miR-107 
                 0.7 
                 0.001260426 
                 0.932 
                 miRNA 
                 135 
               
               
                 ENSG00000199363 
                 0.6 
                 0.001425983 
                 0.750 
                 snoRNA 
                 42 
               
               
                 ACA55 
                 0.5 
                 0.001430114 
                 0.746 
                 HAcaBox 
                 85 
               
               
                 U54 
                 −0.7 
                 0.001511402 
                 0.868 
                 CDBox 
                 135 
               
               
                 U36A 
                 −0.4 
                 0.001519955 
                 0.759 
                 CDBox 
                 132 
               
               
                 U95 
                 −0.7 
                 0.001551616 
                 0.929 
                 CDBox 
                 135 
               
               
                 14qll-22_x 
                 −0.7 
                 0.001591836 
                 0.701 
                 CDBox 
                 50 
               
               
                 U58B_x 
                 −0.5 
                 0.001603166 
                 0.726 
                 CDBox 
                 132 
               
               
                 miR-720 
                 0.7 
                 0.001622509 
                 0.760 
                 miRNA 
                 137 
               
               
                 ENSG00000200394_x 
                 0.5 
                 0.001742151 
                 0.711 
                 snoRNA 
                 115 
               
               
                 HBII-166 
                 −0.5 
                 0.001874456 
                 0.769 
                 CDBox 
                 134 
               
               
                 miR-29b-1-star 
                 1.3 
                 0.001938943 
                 0.736 
                 miRNA 
                 46 
               
               
                 HBII-436 
                 −0.5 
                 0.001978078 
                 0.731 
                 CDBox 
                 102 
               
               
                 U23 
                 −0.5 
                 0.002014594 
                 0.748 
                 HAcaBox 
                 127 
               
               
                 miR-346 
                 −0.9 
                 0.002054981 
                 0.774 
                 miRNA 
                 55 
               
               
                 U58A 
                 −0.5 
                 0.002095349 
                 0.735 
                 CDBox 
                 129 
               
               
                 HBII-95 
                 0.5 
                 0.00219824 
                 0.703 
                 CDBox 
                 78 
               
               
                 miR-339-5p 
                 0.7 
                 0.002357824 
                 0.693 
                 miRNA 
                 75 
               
               
                 ACA25 
                 0.4 
                 0.002440171 
                 0.724 
                 HAcaBox 
                 73 
               
               
                 miR-363 
                 1.1 
                 0.002716266 
                 0.735 
                 miRNA 
                 44 
               
               
                 miR-432 
                 −1.1 
                 0.002752773 
                 0.738 
                 miRNA 
                 51 
               
               
                 miR-424-star 
                 1.1 
                 0.002765097 
                 0.718 
                 miRNA 
                 41 
               
               
                 U27 
                 −0.6 
                 0.002765603 
                 0.792 
                 CDBox 
                 134 
               
               
                 miR-30c 
                 0.6 
                 0.002778453 
                 0.770 
                 miRNA 
                 125 
               
               
                 miR-93 
                 0.7 
                 0.002813996 
                 0.863 
                 miRNA 
                 134 
               
               
                 hsa-let-7a 
                 0.8 
                 0.002856983 
                 0.911 
                 miRNA 
                 136 
               
               
                 miR-181d 
                 1.0 
                 0.002954008 
                 0.748 
                 miRNA 
                 61 
               
               
                 miR-99b 
                 −0.5 
                 0.00296227 
                 0.757 
                 miRNA 
                 129 
               
               
                 miR-550-star 
                 0.9 
                 0.003086147 
                 0.740 
                 miRNA 
                 58 
               
               
                 U43 
                 −0.7 
                 0.00327637 
                 0.865 
                 CDBox 
                 135 
               
               
                 U52 
                 −0.5 
                 0.003332494 
                 0.783 
                 CDBox 
                 135 
               
               
                 miR-502-3p 
                 0.8 
                 0.003628993 
                 0.775 
                 miRNA 
                 110 
               
               
                 spike_in-control-29 
                 −0.4 
                 0.003663934 
                 0.723 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
                   
               
               
                   
                   
                   
                   
                 controls 
                   
               
               
                 14qll-17 
                 −0.7 
                 0.003689916 
                 0.703 
                 CDBox 
                 71 
               
               
                 ENSG00000212302_x 
                 −0.4 
                 0.003876466 
                 0.676 
                 snoRNA 
                 56 
               
               
                 miR-574-3p 
                 −0.7 
                 0.003955226 
                 0.842 
                 miRNA 
                 122 
               
               
                 miR-320d 
                 −0.8 
                 0.004248663 
                 0.868 
                 miRNA 
                 134 
               
               
                 miR-1246 
                 1.0 
                 0.004275913 
                 0.772 
                 miRNA 
                 131 
               
               
                 ACA7_s 
                 −0.4 
                 0.004475709 
                 0.719 
                 HAcaBox 
                 133 
               
               
                 miR-15b 
                 0.6 
                 0.004714973 
                 0.792 
                 miRNA 
                 125 
               
               
                 HBII-52-37_x 
                 −0.5 
                 0.004773282 
                 0.714 
                 CDBox 
                 37 
               
               
                 gi555853_copy0 
                 −0.6 
                 0.004784885 
                 0.799 
                 5.8s rRNA 
                 137 
               
               
                 miR-30e-star 
                 0.8 
                 0.00500785 
                 0.717 
                 miRNA 
                 35 
               
               
                 miR-18a-star 
                 0.8 
                 0.005024206 
                 0.723 
                 miRNA 
                 40 
               
               
                 ENSG00000201847_x 
                 −0.4 
                 0.005059414 
                 0.688 
                 snoRNA 
                 48 
               
               
                 gi555853_copy5 
                 −0.5 
                 0.005220274 
                 0.809 
                 5.8s rRNA 
                 137 
               
               
                 U24 
                 −0.4 
                 0.005634443 
                 0.708 
                 CDBox 
                 133 
               
               
                 miR-1280 
                 −0.4 
                 0.005689807 
                 0.740 
                 miRNA 
                 134 
               
               
                 miR-1231 
                 −0.8 
                 0.005810993 
                 0.676 
                 miRNA 
                 79 
               
               
                 ENSG00000202498_x 
                 −0.5 
                 0.005898128 
                 0.701 
                 snoRNA 
                 137 
               
               
                 gi555853_copy7 
                 −0.5 
                 0.006325433 
                 0.804 
                 5.8s rRNA 
                 137 
               
               
                 miR-28-3p 
                 0.9 
                 0.006506887 
                 0.711 
                 miRNA 
                 94 
               
               
                 ENSG00000199435 
                 0.4 
                 0.006656491 
                 0.706 
                 snoRNA 
                 63 
               
               
                 gi555853_copy2 
                 −0.5 
                 0.00671481 
                 0.800 
                 5.8s rRNA 
                 137 
               
               
                 U43_x 
                 −0.6 
                 0.006913554 
                 0.854 
                 CDBox 
                 135 
               
               
                 ENSG00000212273_x 
                 −0.5 
                 0.007104981 
                 0.725 
                 snoRNA 
                 126 
               
               
                 ENSG00000200961 
                 −0.4 
                 0.007247826 
                 0.716 
                 snoRNA 
                 41 
               
               
                 U38A 
                 −0.7 
                 0.007390068 
                 0.814 
                 CDBox 
                 134 
               
               
                 E3_x 
                 −0.4 
                 0.007704052 
                 0.779 
                 HAcaBox 
                 135 
               
               
                 gi555853_copy8 
                 −0.5 
                 0.00776022 
                 0.799 
                 5.8s rRNA 
                 137 
               
               
                 HBII-234_x 
                 −0.3 
                 0.007814833 
                 0.703 
                 CDBox 
                 102 
               
               
                 U28 
                 −0.5 
                 0.008026327 
                 0.771 
                 CDBox 
                 135 
               
               
                 ENSG00000212587 
                 −0.5 
                 0.008211191 
                 0.685 
                 snoRNA 
                 45 
               
               
                 ENSG00000212149_x 
                 −0.5 
                 0.008233504 
                 0.678 
                 snoRNA 
                 77 
               
               
                 HBII-420 
                 −0.5 
                 0.00828299 
                 0.771 
                 CDBox 
                 131 
               
               
                 gi555853_copy1 
                 −0.5 
                 0.008395769 
                 0.791 
                 5.8s rRNA 
                 137 
               
               
                 HBII-85-21_x 
                 0.5 
                 0.008439987 
                 0.717 
                 CDBox 
                 39 
               
               
                 gi555853_copy4 
                 −0.5 
                 0.008541113 
                 0.793 
                 5.8s rRNA 
                 137 
               
               
                 gi555853_copy6 
                 −0.5 
                 0.008815947 
                 0.792 
                 5.8s rRNA 
                 137 
               
               
                 ENSG00000200897_x 
                 −0.5 
                 0.009024011 
                 0.686 
                 snoRNA 
                 70 
               
               
                 ENSG00000207002 
                 −0.4 
                 0.009046282 
                 0.706 
                 snoRNA 
                 35 
               
               
                 ENSG00000212627 
                 −0.4 
                 0.009345896 
                 0.714 
                 snoRNA 
                 69 
               
               
                 gi555853_copy3 
                 −0.5 
                 0.009628403 
                 0.777 
                 5.8s rRNA 
                 137 
               
               
                 HBII-85-17_x 
                 0.5 
                 0.009677702 
                 0.685 
                 CDBox 
                 57 
               
               
                 miR-92b 
                 0.5 
                 0.009817789 
                 0.672 
                 miRNA 
                 91 
               
               
                 HBII-210 
                 −0.4 
                 0.009959111 
                 0.756 
                 CDBox 
                 135 
               
               
                 ACA3 
                 −0.5 
                 0.010166004 
                 0.740 
                 HAcaBox 
                 134 
               
               
                 miR-769-5p 
                 0.7 
                 0.010207709 
                 0.622 
                 miRNA 
                 56 
               
               
                 U100 
                 0.4 
                 0.010232137 
                 0.645 
                 scaRna 
                 80 
               
               
                 U13 
                 −0.4 
                 0.010247574 
                 0.720 
                 CDBox 
                 135 
               
               
                 miR-181b 
                 0.6 
                 0.010316868 
                 0.816 
                 miRNA 
                 130 
               
               
                 ACA46 
                 0.4 
                 0.010365242 
                 0.699 
                 HAcaBox 
                 124 
               
               
                 gi555853_copy9 
                 −0.5 
                 0.010460125 
                 0.788 
                 5.8s rRNA 
                 137 
               
               
                 U70_x 
                 −0.5 
                 0.011265988 
                 0.704 
                 HAcaBox 
                 53 
               
               
                 HBII-85-15_x 
                 0.5 
                 0.011411104 
                 0.695 
                 CDBox 
                 49 
               
               
                 miR-203 
                 0.7 
                 0.01182754 
                 0.822 
                 miRNA 
                 132 
               
               
                 miR-320c 
                 −0.6 
                 0.011901129 
                 0.845 
                 miRNA 
                 135 
               
               
                 miR-191-star 
                 −0.6 
                 0.012197187 
                 0.680 
                 miRNA 
                 54 
               
               
                 ACA18_x 
                 −0.4 
                 0.012258626 
                 0.694 
                 HAcaBox 
                 135 
               
               
                 miR-27a-star 
                 0.8 
                 0.013060406 
                 0.702 
                 miRNA 
                 40 
               
               
                 miR-192 
                 0.7 
                 0.013123545 
                 0.703 
                 miRNA 
                 32 
               
               
                 U74_x 
                 0.5 
                 0.013418084 
                 0.804 
                 CDBox 
                 135 
               
               
                 ACA15_x 
                 −0.3 
                 0.013532632 
                 0.691 
                 HAcaBox 
                 124 
               
               
                 miR-198 
                 −0.6 
                 0.013839541 
                 0.687 
                 miRNA 
                 31 
               
               
                 U28_x 
                 −0.5 
                 0.013863106 
                 0.711 
                 CDBox 
                 133 
               
               
                 ACA28 
                 0.4 
                 0.013886424 
                 0.685 
                 HAcaBox 
                 120 
               
               
                 U97 
                 −0.4 
                 0.013970203 
                 0.692 
                 CDBox 
                 133 
               
               
                 ACA4 
                 0.3 
                 0.014571497 
                 0.655 
                 HAcaBox 
                 117 
               
               
                 miR-423-5p 
                 −0.6 
                 0.015447652 
                 0.754 
                 miRNA 
                 132 
               
               
                 ACA25_x 
                 0.4 
                 0.015584731 
                 0.701 
                 HAcaBox 
                 114 
               
               
                 HBII-382_s 
                 −0.4 
                 0.016066802 
                 0.670 
                 scaRna 
                 126 
               
               
                 miR-34b 
                 1.2 
                 0.016083233 
                 0.693 
                 miRNA 
                 32 
               
               
                 miR-181a-2-star 
                 0.7 
                 0.016427521 
                 0.646 
                 miRNA 
                 99 
               
               
                 U15B 
                 −0.3 
                 0.017584552 
                 0.689 
                 CDBox 
                 129 
               
               
                 miR-361-5p 
                 0.4 
                 0.017617483 
                 0.783 
                 miRNA 
                 135 
               
               
                 ACA9_x 
                 0.5 
                 0.017621407 
                 0.669 
                 HAcaBox 
                 77 
               
               
                 ACA15_s 
                 −0.3 
                 0.017633335 
                 0.738 
                 HAcaBox 
                 116 
               
               
                 miR-23b-star 
                 0.7 
                 0.018210894 
                 0.672 
                 miRNA 
                 52 
               
               
                 ACA60 
                 −0.3 
                 0.018634054 
                 0.702 
                 HAcaBox 
                 129 
               
               
                 14q-0 
                 −0.4 
                 0.019666039 
                 0.681 
                 CDBox 
                 49 
               
               
                 HBII-85-22_x 
                 0.5 
                 0.019999179 
                 0.673 
                 CDBox 
                 42 
               
               
                 miR-30b-star 
                 0.9 
                 0.020172985 
                 0.686 
                 miRNA 
                 61 
               
               
                 miR-125b 
                 −0.5 
                 0.020376572 
                 0.707 
                 miRNA 
                 135 
               
               
                 ENSG00000199411_s 
                 −0.6 
                 0.020504379 
                 0.779 
                 snoRNA 
                 135 
               
               
                 HBII-251 
                 −0.3 
                 0.020538747 
                 0.679 
                 CDBox 
                 135 
               
               
                 miR-483-3p 
                 0.9 
                 0.020547545 
                 0.666 
                 miRNA 
                 40 
               
               
                 ACA23 
                 −0.3 
                 0.021199129 
                 0.685 
                 HAcaBox 
                 113 
               
               
                 ENSG00000212432_s 
                 −0.4 
                 0.021742945 
                 0.649 
                 snoRNA 
                 75 
               
               
                 miR-25-star 
                 0.7 
                 0.022483461 
                 0.671 
                 miRNA 
                 52 
               
               
                 ENSG00000200932 
                 −0.3 
                 0.023007003 
                 0.687 
                 snoRNA 
                 47 
               
               
                 U49B_s 
                 0.5 
                 0.023157964 
                 0.726 
                 CDBox 
                 129 
               
               
                 ENSG00000212214_x 
                 0.4 
                 0.023504965 
                 0.687 
                 snoRNA 
                 88 
               
               
                 U83B 
                 −0.4 
                 0.024471226 
                 0.745 
                 CDBox 
                 135 
               
               
                 miR-320b 
                 −0.6 
                 0.025686249 
                 0.790 
                 miRNA 
                 135 
               
               
                 miR-489 
                 0.6 
                 0.025963923 
                 0.680 
                 miRNA 
                 58 
               
               
                 miR-26b 
                 0.7 
                 0.026201573 
                 0.682 
                 miRNA 
                 45 
               
               
                 U14B 
                 −0.4 
                 0.027046414 
                 0.632 
                 CDBox 
                 51 
               
               
                 HBII-115 
                 −0.3 
                 0.027115569 
                 0.730 
                 CDBox 
                 117 
               
               
                 ENSG00000207027 
                 −0.4 
                 0.028391297 
                 0.621 
                 snoRNA 
                 36 
               
               
                 miR-494 
                 0.5 
                 0.029570695 
                 0.689 
                 miRNA 
                 135 
               
               
                 miR-181a-star 
                 0.7 
                 0.030787798 
                 0.668 
                 miRNA 
                 35 
               
               
                 U49A_x 
                 0.4 
                 0.03088655 
                 0.750 
                 CDBox 
                 135 
               
               
                 ACA14b_x 
                 −0.3 
                 0.030912946 
                 0.693 
                 HAcaBox 
                 105 
               
               
                 U21 
                 −0.4 
                 0.031731894 
                 0.691 
                 CDBox 
                 134 
               
               
                 HBII-135_x 
                 0.6 
                 0.032076241 
                 0.663 
                 CDBox 
                 132 
               
               
                 miR-382 
                 0.8 
                 0.032245385 
                 0.651 
                 miRNA 
                 47 
               
               
                 miR-532-3p 
                 0.4 
                 0.032767036 
                 0.697 
                 miRNA 
                 95 
               
               
                 miR-214-star 
                 0.8 
                 0.032818193 
                 0.670 
                 miRNA 
                 47 
               
               
                 mgU6-53B 
                 −0.3 
                 0.033293267 
                 0.663 
                 CDBox 
                 99 
               
               
                 miR-200c 
                 −0.7 
                 0.033609817 
                 0.659 
                 miRNA 
                 134 
               
               
                 miR-575 
                 0.5 
                 0.034489882 
                 0.657 
                 miRNA 
                 41 
               
               
                 mgU6-53 
                 −0.3 
                 0.034658143 
                 0.674 
                 CDBox 
                 94 
               
               
                 miR-422a 
                 0.8 
                 0.035772116 
                 0.674 
                 miRNA 
                 84 
               
               
                 ENSG00000201848 
                 −0.3 
                 0.036190647 
                 0.652 
                 snoRNA 
                 34 
               
               
                 ACA45 
                 0.3 
                 0.036575475 
                 0.653 
                 scaRna 
                 53 
               
               
                 miR-559 
                 −0.5 
                 0.036810259 
                 0.647 
                 miRNA 
                 72 
               
               
                 ACA61 
                 −0.4 
                 0.037187144 
                 0.716 
                 HAcaBox 
                 135 
               
               
                 snR38C 
                 −0.4 
                 0.037656608 
                 0.721 
                 CDBox 
                 134 
               
               
                 HBII-85-23_x 
                 0.5 
                 0.037660456 
                 0.650 
                 CDBox 
                 58 
               
               
                 miR-551b-star 
                 −0.5 
                 0.037971736 
                 0.645 
                 miRNA 
                 61 
               
               
                 miR-27b-star 
                 0.7 
                 0.041289539 
                 0.663 
                 miRNA 
                 51 
               
               
                 U50 
                 −0.4 
                 0.041514005 
                 0.696 
                 CDBox 
                 134 
               
               
                 ACA58_x 
                 0.3 
                 0.041793061 
                 0.653 
                 HAcaBox 
                 89 
               
               
                 ACA53 
                 −0.3 
                 0.0421874 
                 0.635 
                 HAcaBox 
                 105 
               
               
                 U49A 
                 0.4 
                 0.042284609 
                 0.694 
                 CDBox 
                 135 
               
               
                 ACA16 
                 −0.4 
                 0.042968027 
                 0.658 
                 HAcaBox 
                 47 
               
               
                 ACA13 
                 −0.4 
                 0.043493352 
                 0.722 
                 HAcaBox 
                 134 
               
               
                 miR-320a 
                 −0.5 
                 0.043683231 
                 0.755 
                 miRNA 
                 135 
               
               
                 ENSG00000200969 
                 −0.4 
                 0.043904585 
                 0.663 
                 snoRNA 
                 67 
               
               
                 miR-642 
                 −0.4 
                 0.04542452 
                 0.640 
                 miRNA 
                 69 
               
               
                 miR-148b 
                 0.5 
                 0.046811178 
                 0.645 
                 miRNA 
                 32 
               
               
                 U84 
                 −0.2 
                 0.046963896 
                 0.683 
                 CDBox 
                 131 
               
               
                 U56_x 
                 0.4 
                 0.047943262 
                 0.701 
                 CDBox 
                 134 
               
               
                 miR-451 
                 0.7 
                 0.049304242 
                 0.632 
                 miRNA 
                 72 
               
               
                 miR-195 
                 0.6 
                 0.049397144 
                 0.698 
                 miRNA 
                 132 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 50 miRNAs most significantly expressed between 
               
               
                 melanoma and normal skin 
               
             
          
           
               
                   
                 Log2- 
                   
                   
                   
                 #Sample 
               
               
                 Probe Name 
                 FC 
                 P value 
                 AUC 
                 ProbeType 
                 Detected 
               
               
                   
               
             
          
           
               
                 miR-146a 
                 4.6 
                 9.43624E−20 
                 0.983 
                 miRNA 
                 129 
               
               
                 miR-509-3p 
                 5.2 
                 5.61558E−17 
                 0.969 
                 miRNA 
                 116 
               
               
                 14qll-14 
                 −2.6 
                 1.16829E−14 
                 0.978 
                 CDBox 
                 56 
               
               
                 miR-25 
                 2.4 
                   2.08E−14 
                 0.949 
                 miRNA 
                 118 
               
               
                 miR-138 
                 3.2 
                 2.51219E−13 
                 0.954 
                 miRNA 
                 82 
               
               
                 miR-509-3-5p 
                 3.6 
                 2.94532E−13 
                 0.963 
                 miRNA 
                 107 
               
               
                 miR-506 
                 3.4 
                 6.99753E−13 
                 0.954 
                 miRNA 
                 76 
               
               
                 14qll-14_x 
                 −2.2 
                 8.36026E−13 
                 0.970 
                 CDBox 
                 56 
               
               
                 14ql-4 
                 −2.1 
                  8.9267E−13 
                 0.964 
                 CDBox 
                 80 
               
               
                 miR-30b 
                 2.2 
                 2.43822E−12 
                 0.957 
                 miRNA 
                 128 
               
               
                 miR-513a-5p 
                 3.5 
                 3.17158E−12 
                 0.939 
                 miRNA 
                 105 
               
               
                 miR-21 
                 3.3 
                 4.58391E−12 
                 0.926 
                 miRNA 
                 79 
               
               
                 Z17B 
                 −1.0 
                 6.92137E−12 
                 0.956 
                 CDBox 
                 117 
               
               
                 U33 
                 −0.9 
                 8.19977E−12 
                 0.968 
                 CDBox 
                 137 
               
               
                 miR-20b 
                 2.8 
                 2.24496E−11 
                 0.939 
                 miRNA 
                 99 
               
               
                 hsa-let-7i 
                 2.3 
                 3.27742E−11 
                 0.973 
                 miRNA 
                 135 
               
               
                 HBII-239 
                 −1.0 
                  3.9295E−11 
                 0.920 
                 CDBox 
                 135 
               
               
                 miR-146b-5p 
                 2.3 
                 8.71488E−11 
                 0.910 
                 miRNA 
                 83 
               
               
                 14qll-26_x 
                 −1.8 
                 9.17329E−11 
                 0.914 
                 CDBox 
                 41 
               
               
                 miR-155 
                 2.8 
                 1.12361E−10 
                 0.940 
                 miRNA 
                 123 
               
               
                 miR-151-3p 
                 1.8 
                  1.7141E−10 
                 0.922 
                 miRNA 
                 117 
               
               
                 HBII-289 
                 −1.1 
                 1.76712E−10 
                 0.963 
                 CDBox 
                 135 
               
               
                 14qll-12_x 
                 −1.7 
                  1.9475E−10 
                 0.907 
                 CDBox 
                 85 
               
               
                 miR-1274a 
                 1.9 
                 3.28343E−10 
                 0.928 
                 miRNA 
                 48 
               
               
                 HBII-180A_x 
                 −0.8 
                 4.68853E−10 
                 0.933 
                 CDBox 
                 123 
               
               
                 miR-1301 
                 2.0 
                 7.22101E−10 
                 0.896 
                 miRNA 
                 54 
               
               
                 14qll-1_x 
                 −1.7 
                 7.72545E−10 
                 0.965 
                 CDBox 
                 131 
               
               
                 miR-193b 
                 −1.4 
                  8.7852E−10 
                 0.974 
                 miRNA 
                 130 
               
               
                 miR-510 
                 2.7 
                 1.03096E−09 
                 0.914 
                 miRNA 
                 82 
               
               
                 miR-126 
                 2.1 
                 1.35297E−09 
                 0.959 
                 miRNA 
                 133 
               
               
                 miR-24-2-star 
                 1.8 
                 1.36986E−09 
                 0.895 
                 miRNA 
                 63 
               
               
                 miR-106b 
                 2.1 
                 1.91606E−09 
                 0.930 
                 miRNA 
                 127 
               
               
                 HBII-276 
                 −1.0 
                 2.06051E−09 
                 0.908 
                 CDBox 
                 132 
               
               
                 miR-532-5p 
                 1.8 
                  2.9654E−09 
                 0.887 
                 miRNA 
                 113 
               
               
                 14qll-12 
                 −1.7 
                 3.71443E−09 
                 0.859 
                 CDBox 
                 58 
               
               
                 miR-19b 
                 2.1 
                 3.71682E−09 
                 0.908 
                 miRNA 
                 124 
               
               
                 miR-30a 
                 1.6 
                 6.61873E−09 
                 0.894 
                 miRNA 
                 113 
               
               
                 HBII-85-26_x 
                 −1.1 
                 7.76845E−09 
                 0.895 
                 CDBox 
                 135 
               
               
                 miR-150 
                 1.7 
                 1.08172E−08 
                 0.894 
                 miRNA 
                 117 
               
               
                 14qll-26 
                 −1.5 
                 1.16704E−08 
                 0.869 
                 CDBox 
                 32 
               
               
                 miR-324-5p 
                 1.6 
                 1.24724E−08 
                 0.880 
                 miRNA 
                 102 
               
               
                 14ql-8 
                 −1.2 
                 1.39067E−08 
                 0.894 
                 CDBox 
                 61 
               
               
                 miR-185 
                 1.6 
                 1.44139E−08 
                 0.952 
                 miRNA 
                 132 
               
               
                 miR-194 
                 1.8 
                 1.72961E−08 
                 0.883 
                 miRNA 
                 54 
               
               
                 14qll-1 
                 −1.5 
                 2.76507E−08 
                 0.928 
                 CDBox 
                 128 
               
               
                 HBII-202 
                 −1.0 
                 2.89826E−08 
                 0.946 
                 CDBox 
                 135 
               
               
                 miR-768-5p 
                 −1.6 
                  3.1365E−08 
                 0.986 
                 miRNA 
                 135 
               
               
                 miR-421 
                 1.6 
                 4.42916E−08 
                 0.872 
                 miRNA 
                 68 
               
               
                 miR-28-5p 
                 1.7 
                 5.45027E−08 
                 0.877 
                 miRNA 
                 105 
               
               
                 miR-151-5p 
                 1.2 
                 5.48542E−08 
                 0.968 
                 miRNA 
                 135 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 miRNAs significantly expressed between metastatic melanoma and melanoma 
               
             
          
           
               
                   
                 Log2- 
                   
                   
                   
                 #Sample 
               
               
                 Probe Name 
                 FC 
                 P value 
                 AUC 
                 ProbeType 
                 Detected 
               
               
                   
               
             
          
           
               
                 miR-31 
                 −2.4 
                 6.23649E−09 
                 0.891 
                 miRNA 
                 103 
               
               
                 miR-150 
                 −1.2 
                 4.84574E−06 
                 0.783 
                 miRNA 
                 117 
               
               
                 miR-203 
                 −2.0 
                 2.77555E−05 
                 0.764 
                 miRNA 
                 132 
               
               
                 ENSG00000212139_x 
                 0.6 
                 2.83257E−05 
                 0.799 
                 snoRNA 
                 124 
               
               
                 mgU6-53 
                 0.6 
                  3.8628E−05 
                 0.828 
                 CDBox 
                 94 
               
               
                 U72_x 
                 0.5 
                 4.47709E−05 
                 0.783 
                 HAcaBox 
                 103 
               
               
                 U94 
                 0.6 
                 7.34024E−05 
                 0.776 
                 CDBox 
                 109 
               
               
                 HBII-85-15_x 
                 0.8 
                 0.000176472 
                 0.767 
                 CDBox 
                 49 
               
               
                 HBII-85-29_x 
                 0.8 
                 0.000274959 
                 0.734 
                 CDBox 
                 85 
               
               
                 HBII-55 
                 0.5 
                 0.000331716 
                 0.806 
                 CDBox 
                 135 
               
               
                 snR38B 
                 0.8 
                 0.000374886 
                 0.750 
                 CDBox 
                 126 
               
               
                 miR-200c 
                 −1.6 
                 0.00042063 
                 0.718 
                 miRNA 
                 134 
               
               
                 U61 
                 0.7 
                 0.000440758 
                 0.785 
                 CDBox 
                 116 
               
               
                 HBII-316 
                 0.6 
                 0.000548042 
                 0.744 
                 CDBox 
                 130 
               
               
                 U32A_x 
                 0.4 
                 0.000553879 
                 0.748 
                 CDBox 
                 136 
               
               
                 U81_x 
                 0.6 
                 0.000598062 
                 0.720 
                 CDBox 
                 120 
               
               
                 U15A 
                 0.7 
                 0.000649777 
                 0.785 
                 CDBox 
                 128 
               
               
                 ACA14b_x 
                 0.4 
                 0.000764147 
                 0.748 
                 HAcaBox 
                 105 
               
               
                 miR-182 
                 −0.9 
                 0.000868427 
                 0.762 
                 miRNA 
                 113 
               
               
                 miR-455-3p 
                 −0.7 
                 0.000874654 
                 0.718 
                 miRNA 
                 136 
               
               
                 miR-532-5p 
                 −0.8 
                 0.000883675 
                 0.752 
                 miRNA 
                 113 
               
               
                 mgU6-53_x 
                 0.5 
                 0.001038416 
                 0.783 
                 CDBox 
                 113 
               
               
                 ACA46 
                 0.4 
                 0.001076758 
                 0.746 
                 HAcaBox 
                 124 
               
               
                 miR-1234 
                 −0.5 
                 0.001112272 
                 0.756 
                 miRNA 
                 68 
               
               
                 U53 
                 0.6 
                 0.001130502 
                 0.769 
                 CDBox 
                 126 
               
               
                 HBII-85-29 
                 0.7 
                 0.001192467 
                 0.702 
                 CDBox 
                 82 
               
               
                 U47 
                 0.5 
                 0.001218674 
                 0.702 
                 CDBox 
                 74 
               
               
                 U42B_x 
                 0.5 
                 0.001412559 
                 0.735 
                 CDBox 
                 109 
               
               
                 miR-155 
                 −1.3 
                 0.001425842 
                 0.716 
                 miRNA 
                 123 
               
               
                 U64 
                 0.5 
                 0.001493551 
                 0.728 
                 HAcaBox 
                 101 
               
               
                 ACA28 
                 0.5 
                 0.001565276 
                 0.692 
                 HAcaBox 
                 120 
               
               
                 U13 
                 0.6 
                 0.001630341 
                 0.748 
                 CDBox 
                 135 
               
               
                 U84 
                 0.4 
                 0.001673776 
                 0.769 
                 CDBox 
                 131 
               
               
                 U22 
                 0.5 
                 0.001739769 
                 0.714 
                 CDBox 
                 135 
               
               
                 U46_x 
                 0.6 
                 0.001758635 
                 0.755 
                 CDBox 
                 131 
               
               
                 ACA17_x 
                 0.6 
                 0.001827243 
                 0.705 
                 HAcaBox 
                 44 
               
               
                 ENSG00000200897 
                 0.4 
                 0.001857043 
                 0.735 
                 snoRNA 
                 38 
               
               
                 U36A_x 
                 0.4 
                 0.00192703 
                 0.728 
                 CDBox 
                 133 
               
               
                 HBII-180C 
                 0.4 
                 0.001987135 
                 0.757 
                 CDBox 
                 115 
               
               
                 HBII-251 
                 0.4 
                 0.0020213 
                 0.741 
                 CDBox 
                 135 
               
               
                 ACA49 
                 0.4 
                 0.002175278 
                 0.744 
                 HAcaBox 
                 127 
               
               
                 U19 
                 0.6 
                 0.00221226 
                 0.696 
                 HAcaBox 
                 118 
               
               
                 miR-940 
                 −0.6 
                 0.002377881 
                 0.713 
                 miRNA 
                 53 
               
               
                 U76 
                 0.6 
                 0.002408733 
                 0.755 
                 CDBox 
                 135 
               
               
                 HBII-13_x 
                 0.7 
                 0.002521867 
                 0.730 
                 CDBox 
                 60 
               
               
                 HBII-382_s 
                 0.6 
                 0.002554678 
                 0.770 
                 scaRna 
                 126 
               
               
                 miR-205 
                 −1.1 
                 0.002610442 
                 0.603 
                 miRNA 
                 135 
               
               
                 U21 
                 0.7 
                 0.002613305 
                 0.774 
                 CDBox 
                 134 
               
               
                 miR-342-3p 
                 −0.5 
                 0.002621512 
                 0.706 
                 miRNA 
                 136 
               
               
                 ENSG00000212326 
                 0.5 
                 0.002879558 
                 0.728 
                 snoRNA 
                 104 
               
               
                 U82 
                 0.7 
                 0.002979246 
                 0.828 
                 CDBox 
                 131 
               
               
                 HBII-234_x 
                 0.3 
                 0.003087142 
                 0.734 
                 CDBox 
                 102 
               
               
                 HBII-85-24_x 
                 0.4 
                 0.003172633 
                 0.703 
                 CDBox 
                 34 
               
               
                 U101 
                 0.5 
                 0.003644287 
                 0.672 
                 CDBox 
                 134 
               
               
                 U46 
                 0.5 
                 0.003690792 
                 0.747 
                 CDBox 
                 133 
               
               
                 U58A 
                 0.5 
                 0.003778891 
                 0.735 
                 CDBox 
                 129 
               
               
                 HBII-210 
                 0.5 
                 0.003785668 
                 0.767 
                 CDBox 
                 135 
               
               
                 U102 
                 0.4 
                 0.00401337 
                 0.705 
                 CDBox 
                 124 
               
               
                 HBII-85-3_x 
                 0.5 
                 0.004030857 
                 0.694 
                 CDBox 
                 72 
               
               
                 U92 
                 0.4 
                 0.004575813 
                 0.712 
                 scaRna 
                 129 
               
               
                 spike_in-control-31 
                 0.1 
                 0.004779896 
                 0.705 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 snR38C 
                 0.6 
                 0.005413855 
                 0.779 
                 CDBox 
                 134 
               
               
                 HBII-99 
                 0.5 
                 0.005634509 
                 0.714 
                 CDBox 
                 122 
               
               
                 ACA47 
                 0.5 
                 0.006095831 
                 0.696 
                 scaRna 
                 77 
               
               
                 HBII-420 
                 0.6 
                 0.006404916 
                 0.824 
                 CDBox 
                 131 
               
               
                 ENSG00000212423_x 
                 0.5 
                 0.006424898 
                 0.684 
                 snoRNA 
                 115 
               
               
                 HBII-85-17_x 
                 0.6 
                 0.006577979 
                 0.678 
                 CDBox 
                 57 
               
               
                 ACA27_x 
                 0.3 
                 0.006580858 
                 0.725 
                 HAcaBox 
                 130 
               
               
                 U79 
                 0.6 
                 0.007498133 
                 0.748 
                 CDBox 
                 134 
               
               
                 U107 
                 0.4 
                 0.00761608 
                 0.707 
                 HAcaBox 
                 129 
               
               
                 HBI-43 
                 0.3 
                 0.007872874 
                 0.691 
                 CDBox 
                 122 
               
               
                 U49B_x 
                 0.5 
                 0.008037057 
                 0.736 
                 CDBox 
                 122 
               
               
                 miR-548i 
                 −0.5 
                 0.008242048 
                 0.684 
                 miRNA 
                 36 
               
               
                 miR-500-star 
                 −0.6 
                 0.008434274 
                 0.697 
                 miRNA 
                 97 
               
               
                 HBII-85-2_x 
                 0.4 
                 0.008511275 
                 0.708 
                 CDBox 
                 135 
               
               
                 ACA41 
                 0.5 
                 0.008991193 
                 0.723 
                 HAcaBox 
                 115 
               
               
                 ACA3 
                 0.5 
                 0.009231708 
                 0.737 
                 HAcaBox 
                 134 
               
               
                 U49A 
                 0.6 
                 0.009381377 
                 0.745 
                 CDBox 
                 135 
               
               
                 HBII-85-20_x 
                 0.5 
                 0.00942941 
                 0.672 
                 CDBox 
                 42 
               
               
                 ENSG00000212508 
                 −0.5 
                 0.009471802 
                 0.712 
                 snoRNA 
                 122 
               
               
                 U38B_x 
                 0.7 
                 0.009547747 
                 0.837 
                 CDBox 
                 133 
               
               
                 ACA51_x 
                 0.3 
                 0.009599762 
                 0.686 
                 HAcaBox 
                 135 
               
               
                 ENSG00000200879 
                 0.5 
                 0.009860049 
                 0.697 
                 snoRNA 
                 120 
               
               
                 miR-181a 
                 −0.6 
                 0.009890715 
                 0.730 
                 miRNA 
                 134 
               
               
                 ENSG00000200932 
                 0.4 
                 0.010075893 
                 0.740 
                 snoRNA 
                 47 
               
               
                 miR-589-star 
                 −0.4 
                 0.010240715 
                 0.650 
                 miRNA 
                 43 
               
               
                 ENSG00000199262 
                 0.4 
                 0.010309025 
                 0.694 
                 snoRNA 
                 59 
               
               
                 miR-1324 
                 −0.5 
                 0.010648472 
                 0.724 
                 miRNA 
                 57 
               
               
                 U91_s 
                 0.5 
                 0.010903574 
                 0.695 
                 scaRna 
                 132 
               
               
                 U3-2_s 
                 0.5 
                 0.011269742 
                 0.729 
                 CDBox 
                 135 
               
               
                 U51_x 
                 0.4 
                 0.011519055 
                 0.713 
                 CDBox 
                 130 
               
               
                 ACA9 
                 0.5 
                 0.012046848 
                 0.703 
                 HAcaBox 
                 82 
               
               
                 miR-1825 
                 −0.8 
                 0.01292318 
                 0.738 
                 miRNA 
                 132 
               
               
                 mgU6-47 
                 0.4 
                 0.013536315 
                 0.710 
                 CDBox 
                 47 
               
               
                 miR-1281 
                 −0.7 
                 0.014086281 
                 0.701 
                 miRNA 
                 137 
               
               
                 U71d_x 
                 0.5 
                 0.01413005 
                 0.678 
                 HAcaBox 
                 99 
               
               
                 ACA42 
                 0.4 
                 0.014283243 
                 0.786 
                 HAcaBox 
                 117 
               
               
                 ACA34 
                 0.3 
                 0.014399902 
                 0.637 
                 HAcaBox 
                 109 
               
               
                 U48 
                 0.4 
                 0.014650847 
                 0.654 
                 CDBox 
                 129 
               
               
                 ENSG00000202093 
                 0.5 
                 0.014675533 
                 0.689 
                 snoRNA 
                 97 
               
               
                 miR-665 
                 0.5 
                 0.014853517 
                 0.678 
                 miRNA 
                 47 
               
               
                 miR-141 
                 −0.7 
                 0.015316578 
                 0.647 
                 miRNA 
                 69 
               
               
                 U23 
                 0.4 
                 0.015408258 
                 0.701 
                 HAcaBox 
                 127 
               
               
                 U15B 
                 0.4 
                 0.015684928 
                 0.719 
                 CDBox 
                 129 
               
               
                 miR-1274b 
                 0.6 
                 0.01695735 
                 0.679 
                 miRNA 
                 112 
               
               
                 U31_x 
                 0.5 
                 0.017818548 
                 0.771 
                 CDBox 
                 135 
               
               
                 ENSG00000200394_x 
                 0.4 
                 0.017859341 
                 0.682 
                 snoRNA 
                 115 
               
               
                 ACA67_x 
                 0.4 
                 0.018242565 
                 0.663 
                 HAcaBox 
                 54 
               
               
                 U80 
                 0.4 
                 0.019116778 
                 0.683 
                 CDBox 
                 132 
               
               
                 HBII-85-18_x 
                 0.4 
                 0.019329528 
                 0.661 
                 CDBox 
                 48 
               
               
                 U106 
                 0.3 
                 0.019924806 
                 0.653 
                 CDBox 
                 112 
               
               
                 U99 
                 0.3 
                 0.01993828 
                 0.674 
                 HAcaBox 
                 137 
               
               
                 U24 
                 0.3 
                 0.020062119 
                 0.673 
                 CDBox 
                 133 
               
               
                 miR-20a-star 
                 1.0 
                 0.020518991 
                 0.694 
                 miRNA 
                 61 
               
               
                 U16 
                 0.4 
                 0.0213066 
                 0.669 
                 CDBox 
                 131 
               
               
                 U49B_s 
                 0.5 
                 0.0213349 
                 0.716 
                 CDBox 
                 129 
               
               
                 miR-885-3p 
                 0.7 
                 0.021367498 
                 0.694 
                 miRNA 
                 73 
               
               
                 U36B 
                 0.4 
                 0.021413004 
                 0.669 
                 CDBox 
                 121 
               
               
                 HBII-295 
                 0.3 
                 0.021589703 
                 0.678 
                 CDBox 
                 128 
               
               
                 miR-148b 
                 0.6 
                 0.021711762 
                 0.661 
                 miRNA 
                 32 
               
               
                 U38B 
                 0.7 
                 0.022451358 
                 0.834 
                 CDBox 
                 134 
               
               
                 U31 
                 0.6 
                 0.023045818 
                 0.738 
                 CDBox 
                 132 
               
               
                 ACA8_x 
                 0.4 
                 0.023508719 
                 0.697 
                 HAcaBox 
                 125 
               
               
                 HBI-6_x 
                 0.5 
                 0.02438732 
                 0.675 
                 HAcaBox 
                 115 
               
               
                 mgU6-53B 
                 0.3 
                 0.024830223 
                 0.687 
                 CDBox 
                 99 
               
               
                 ACA62 
                 0.4 
                 0.024948935 
                 0.714 
                 HAcaBox 
                 88 
               
               
                 miR-23b 
                 −0.5 
                 0.025010006 
                 0.658 
                 miRNA 
                 137 
               
               
                 mgh18S-121 
                 0.4 
                 0.02717981 
                 0.773 
                 CDBox 
                 134 
               
               
                 miR-134 
                 −0.6 
                 0.027498011 
                 0.648 
                 miRNA 
                 61 
               
               
                 ENSG00000202216 
                 −0.3 
                 0.02787169 
                 0.654 
                 snoRNA 
                 53 
               
               
                 snR38A 
                 0.5 
                 0.028058616 
                 0.706 
                 CDBox 
                 130 
               
               
                 ACA54 
                 0.4 
                 0.028079033 
                 0.741 
                 HAcaBox 
                 133 
               
               
                 U83 
                 0.4 
                 0.028849489 
                 0.733 
                 CDBox 
                 135 
               
               
                 U56_x 
                 0.5 
                 0.03056515 
                 0.673 
                 CDBox 
                 134 
               
               
                 ENSG00000207118 
                 0.5 
                 0.030731527 
                 0.642 
                 snoRNA 
                 93 
               
               
                 U71b_x 
                 0.3 
                 0.031331331 
                 0.639 
                 HAcaBox 
                 117 
               
               
                 U104 
                 0.5 
                 0.031533136 
                 0.750 
                 CDBox 
                 135 
               
               
                 miR-26b 
                 0.6 
                 0.031546885 
                 0.666 
                 miRNA 
                 45 
               
               
                 ENSG00000201129 
                 0.4 
                 0.031647545 
                 0.658 
                 snoRNA 
                 54 
               
               
                 HBII-180C_x 
                 0.3 
                 0.031765824 
                 0.686 
                 CDBox 
                 131 
               
               
                 miR-1257 
                 −0.4 
                 0.032490122 
                 0.676 
                 miRNA 
                 31 
               
               
                 miR-1285 
                 0.6 
                 0.032817723 
                 0.656 
                 miRNA 
                 54 
               
               
                 ACA6 
                 0.4 
                 0.033332825 
                 0.706 
                 HAcaBox 
                 126 
               
               
                 HBII-166 
                 0.3 
                 0.033479679 
                 0.715 
                 CDBox 
                 134 
               
               
                 U20 
                 0.3 
                 0.033581135 
                 0.659 
                 CDBox 
                 117 
               
               
                 U49A_x 
                 0.5 
                 0.034235707 
                 0.734 
                 CDBox 
                 135 
               
               
                 HBII-85-11 
                 0.5 
                 0.034401945 
                 0.626 
                 CDBox 
                 31 
               
               
                 miR-198 
                 0.5 
                 0.035071443 
                 0.673 
                 miRNA 
                 31 
               
               
                 miR-1273 
                 0.4 
                 0.035126423 
                 0.649 
                 miRNA 
                 47 
               
               
                 U8_x 
                 0.4 
                 0.035369399 
                 0.661 
                 CDBox 
                 135 
               
               
                 miR-127-3p 
                 −0.5 
                 0.035478749 
                 0.646 
                 miRNA 
                 89 
               
               
                 U36C 
                 0.3 
                 0.036400622 
                 0.690 
                 CDBox 
                 135 
               
               
                 miR-502-3p 
                 −0.5 
                 0.036440109 
                 0.696 
                 miRNA 
                 110 
               
               
                 U97 
                 0.4 
                 0.036556318 
                 0.713 
                 CDBox 
                 133 
               
               
                 HBII-13 
                 0.5 
                 0.038037592 
                 0.639 
                 CDBox 
                 52 
               
               
                 miR-148a 
                 0.6 
                 0.038972124 
                 0.647 
                 miRNA 
                 41 
               
               
                 hsa-let-7b 
                 −0.7 
                 0.040092161 
                 0.713 
                 miRNA 
                 136 
               
               
                 U55 
                 0.3 
                 0.041929175 
                 0.687 
                 CDBox 
                 135 
               
               
                 HBII-85-5_x 
                 0.3 
                 0.04234391 
                 0.645 
                 CDBox 
                 31 
               
               
                 U105 
                 0.3 
                 0.043199784 
                 0.658 
                 CDBox 
                 126 
               
               
                 U90 
                 0.3 
                 0.043617609 
                 0.701 
                 scaRna 
                 114 
               
               
                 ENSG00000202252 
                 0.3 
                 0.044418964 
                 0.647 
                 snoRNA 
                 135 
               
               
                 ENSG00000201467_x 
                 −0.3 
                 0.04502792 
                 0.628 
                 snoRNA 
                 31 
               
               
                 ACA21 
                 0.5 
                 0.045481211 
                 0.754 
                 HAcaBox 
                 133 
               
               
                 U52 
                 0.4 
                 0.045645643 
                 0.708 
                 CDBox 
                 135 
               
               
                 U18C_x 
                 0.4 
                 0.046127928 
                 0.637 
                 CDBox 
                 74 
               
               
                 HBII-296B 
                 0.3 
                 0.046365102 
                 0.657 
                 CDBox 
                 98 
               
               
                 ENSG00000200307 
                 0.3 
                 0.048211492 
                 0.613 
                 snoRNA 
                 86 
               
               
                 miR-509-3p 
                 −1.2 
                 0.048257749 
                 0.574 
                 miRNA 
                 116 
               
               
                 miR-1202 
                 0.5 
                 0.048318042 
                 0.661 
                 miRNA 
                 57 
               
               
                 ACA36_x 
                 0.4 
                 0.048990385 
                 0.670 
                 HAcaBox 
                 88 
               
               
                 ENSG00000207016_x 
                 0.3 
                 0.049618219 
                 0.660 
                 snoRNA 
                 48 
               
               
                 miR-30c 
                 0.3 
                 0.049698 
                 0.709 
                 miRNA 
                 125 
               
               
                 ACA19 
                 0.3 
                 0.049825075 
                 0.655 
                 HAcaBox 
                 118 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 50 miRNAs most significantly expressed between metastatic melanoma and melanoma 
               
             
          
           
               
                   
                 Log2- 
                   
                   
                   
                   
               
               
                 Probe Name 
                 FC 
                 P value 
                 AUC 
                 ProbeType 
                 #Sample Detected 
               
               
                   
               
             
          
           
               
                 miR-31 
                 −2.4 
                 6.23649E−09 
                 0.891 
                 miRNA 
                 103 
               
               
                 miR-150 
                 −1.2 
                 4.84574E−06 
                 0.783 
                 miRNA 
                 117 
               
               
                 miR-203 
                 −2.0 
                 2.77555E−05 
                 0.764 
                 miRNA 
                 132 
               
               
                 ENSG00000212139_x 
                 0.6 
                 2.83257E−05 
                 0.799 
                 snoRNA 
                 124 
               
               
                 mgU6-53 
                 0.6 
                  3.8628E−05 
                 0.828 
                 CDBox 
                 94 
               
               
                 U72_x 
                 0.5 
                 4.47709E−05 
                 0.783 
                 HAcaBox 
                 103 
               
               
                 U94 
                 0.6 
                 7.34024E−05 
                 0.776 
                 CDBox 
                 109 
               
               
                 HBII-85-15_x 
                 0.8 
                 0.000176472 
                 0.767 
                 CDBox 
                 49 
               
               
                 HBII-85-29_x 
                 0.8 
                 0.000274959 
                 0.734 
                 CDBox 
                 85 
               
               
                 HBII-55 
                 0.5 
                 0.000331716 
                 0.806 
                 CDBox 
                 135 
               
               
                 snR38B 
                 0.8 
                 0.000374886 
                 0.750 
                 CDBox 
                 126 
               
               
                 miR-200c 
                 −1.6 
                 0.00042063 
                 0.718 
                 miRNA 
                 134 
               
               
                 U61 
                 0.7 
                 0.000440758 
                 0.785 
                 CDBox 
                 116 
               
               
                 HBII-316 
                 0.6 
                 0.000548042 
                 0.744 
                 CDBox 
                 130 
               
               
                 U32A_x 
                 0.4 
                 0.000553879 
                 0.748 
                 CDBox 
                 136 
               
               
                 U81_x 
                 0.6 
                 0.000598062 
                 0.720 
                 CDBox 
                 120 
               
               
                 U15A 
                 0.7 
                 0.000649777 
                 0.785 
                 CDBox 
                 128 
               
               
                 ACA14b_x 
                 0.4 
                 0.000764147 
                 0.748 
                 HAcaBox 
                 105 
               
               
                 miR-182 
                 −0.9 
                 0.000868427 
                 0.762 
                 miRNA 
                 113 
               
               
                 miR-455-3p 
                 −0.7 
                 0.000874654 
                 0.718 
                 miRNA 
                 136 
               
               
                 miR-532-5p 
                 −0.8 
                 0.000883675 
                 0.752 
                 miRNA 
                 113 
               
               
                 mgU6-53_x 
                 0.5 
                 0.001038416 
                 0.783 
                 CDBox 
                 113 
               
               
                 ACA46 
                 0.4 
                 0.001076758 
                 0.746 
                 HAcaBox 
                 124 
               
               
                 miR-1234 
                 −0.5 
                 0.001112272 
                 0.756 
                 miRNA 
                 68 
               
               
                 U53 
                 0.6 
                 0.001130502 
                 0.769 
                 CDBox 
                 126 
               
               
                 HBII-85-29 
                 0.7 
                 0.001192467 
                 0.702 
                 CDBox 
                 82 
               
               
                 U47 
                 0.5 
                 0.001218674 
                 0.702 
                 CDBox 
                 74 
               
               
                 U42B_x 
                 0.5 
                 0.001412559 
                 0.735 
                 CDBox 
                 109 
               
               
                 miR-155 
                 −1.3 
                 0.001425842 
                 0.716 
                 miRNA 
                 123 
               
               
                 U64 
                 0.5 
                 0.001493551 
                 0.728 
                 HAcaBox 
                 101 
               
               
                 ACA28 
                 0.5 
                 0.001565276 
                 0.692 
                 HAcaBox 
                 120 
               
               
                 U13 
                 0.6 
                 0.001630341 
                 0.748 
                 CDBox 
                 135 
               
               
                 U84 
                 0.4 
                 0.001673776 
                 0.769 
                 CDBox 
                 131 
               
               
                 U22 
                 0.5 
                 0.001739769 
                 0.714 
                 CDBox 
                 135 
               
               
                 U46_x 
                 0.6 
                 0.001758635 
                 0.755 
                 CDBox 
                 131 
               
               
                 ACA17_x 
                 0.6 
                 0.001827243 
                 0.705 
                 HAcaBox 
                 44 
               
               
                 ENSG00000200897 
                 0.4 
                 0.001857043 
                 0.735 
                 snoRNA 
                 38 
               
               
                 U36A_x 
                 0.4 
                 0.00192703 
                 0.728 
                 CDBox 
                 133 
               
               
                 HBII-180C 
                 0.4 
                 0.001987135 
                 0.757 
                 CDBox 
                 115 
               
               
                 HBII-251 
                 0.4 
                 0.0020213 
                 0.741 
                 CDBox 
                 135 
               
               
                 ACA49 
                 0.4 
                 0.002175278 
                 0.744 
                 HAcaBox 
                 127 
               
               
                 U19 
                 0.6 
                 0.00221226 
                 0.696 
                 HAcaBox 
                 118 
               
               
                 miR-940 
                 −0.6 
                 0.002377881 
                 0.713 
                 miRNA 
                 53 
               
               
                 U76 
                 0.6 
                 0.002408733 
                 0.755 
                 CDBox 
                 135 
               
               
                 HBII-13_x 
                 0.7 
                 0.002521867 
                 0.730 
                 CDBox 
                 60 
               
               
                 HBII-382_s 
                 0.6 
                 0.002554678 
                 0.770 
                 scaRna 
                 126 
               
               
                 miR-205 
                 −1.1 
                 0.002610442 
                 0.603 
                 miRNA 
                 135 
               
               
                 U21 
                 0.7 
                 0.002613305 
                 0.774 
                 CDBox 
                 134 
               
               
                 miR-342-3p 
                 −0.5 
                 0.002621512 
                 0.706 
                 miRNA 
                 136 
               
               
                 ENSG00000212326 
                 0.5 
                 0.002879558 
                 0.728 
                 snoRNA 
                 104 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 miRNAs significantly expressed between nevi and normal skin 
               
             
          
           
               
                   
                 Log2- 
                   
                   
                   
                 #Sample 
               
               
                 Probe Name 
                 FC 
                 P value 
                 AUC 
                 ProbeType 
                 Detected 
               
               
                   
               
             
          
           
               
                 14qll-14 
                 −3.87 
                  3.6592E−27 
                 1.000 
                 CDBox 
                 56 
               
               
                 14qll-14_x 
                 −3.21 
                  3.815E−24 
                 1.000 
                 CDBox 
                 56 
               
               
                 U74_x 
                 1.46 
                 3.89068E−23 
                 1.000 
                 CDBox 
                 135 
               
               
                 miR-509-3p 
                 5.78 
                 1.67661E−22 
                 0.997 
                 miRNA 
                 116 
               
               
                 miR-768-5p 
                 −1.46 
                 5.23441E−22 
                 1.000 
                 miRNA 
                 135 
               
               
                 ENSG00000199411_s 
                 1.36 
                 1.56796E−21 
                 1.000 
                 snoRNA 
                 135 
               
               
                 Z17B 
                 −1.94 
                 2.89347E−20 
                 0.997 
                 CDBox 
                 117 
               
               
                 miR-149 
                 −3.46 
                 1.55425E−19 
                 1.000 
                 miRNA 
                 100 
               
               
                 miR-125a-5p 
                 −3.44 
                 1.86526E−19 
                 1.000 
                 miRNA 
                 128 
               
               
                 U43 
                 −1.19 
                 3.32514E−19 
                 1.000 
                 CDBox 
                 135 
               
               
                 miR-1308 
                 1.55 
                  8.1184E−19 
                 0.964 
                 miRNA 
                 135 
               
               
                 U59A 
                 1.13 
                 9.99768E−18 
                 0.993 
                 CDBox 
                 135 
               
               
                 miR-146a 
                 4.44 
                 3.16232E−17 
                 0.975 
                 miRNA 
                 129 
               
               
                 miR-513a-5p 
                 3.77 
                 1.05679E−16 
                 0.972 
                 miRNA 
                 105 
               
               
                 ACA20 
                 −1.74 
                 1.56297E−16 
                 0.993 
                 HAcaBox 
                 131 
               
               
                 U43_x 
                 −1.05 
                 1.04459E−15 
                 1.000 
                 CDBox 
                 135 
               
               
                 U44_x 
                 −1.37 
                 5.54123E−15 
                 0.994 
                 CDBox 
                 135 
               
               
                 miR-127-3p 
                 −3.56 
                 7.53549E−15 
                 0.988 
                 miRNA 
                 89 
               
               
                 miR-193b 
                 −2.57 
                 7.81928E−15 
                 1.000 
                 miRNA 
                 130 
               
               
                 U83 
                 −1.25 
                 9.30493E−15 
                 0.993 
                 CDBox 
                 135 
               
               
                 miR-768-3p 
                 0.98 
                 2.39925E−14 
                 0.972 
                 miRNA 
                 137 
               
               
                 U33 
                 −1.02 
                 2.44194E−14 
                 0.997 
                 CDBox 
                 137 
               
               
                 miR-574-3p 
                 −3.05 
                  3.0433E−14 
                 0.974 
                 miRNA 
                 122 
               
               
                 ACA16 
                 −1.86 
                 3.35128E−14 
                 0.968 
                 HAcaBox 
                 47 
               
               
                 14ql-4 
                 −2.43 
                 3.96317E−14 
                 0.979 
                 CDBox 
                 80 
               
               
                 hsa-let-7c 
                 −1.01 
                 4.99111E−14 
                 0.986 
                 miRNA 
                 135 
               
               
                 miR-342-3p 
                 −2.06 
                 1.67074E−13 
                 0.996 
                 miRNA 
                 136 
               
               
                 hsa-let-7b 
                 −1.01 
                 2.25904E−13 
                 0.964 
                 miRNA 
                 136 
               
               
                 miR-423-3p 
                 −2.52 
                 2.97967E−13 
                 1.000 
                 miRNA 
                 107 
               
               
                 HBII-239 
                 −1.41 
                  3.2162E−13 
                 0.975 
                 CDBox 
                 135 
               
               
                 U38B_x 
                 1.52 
                 3.80613E−13 
                 0.968 
                 CDBox 
                 133 
               
               
                 miR-320b 
                 −0.63 
                 6.31909E−13 
                 0.971 
                 miRNA 
                 135 
               
               
                 miR-923 
                 0.93 
                 6.48332E−13 
                 0.990 
                 miRNA 
                 135 
               
               
                 U54 
                 −1.06 
                 1.17197E−12 
                 0.975 
                 CDBox 
                 135 
               
               
                 U38B 
                 1.80 
                 1.18885E−12 
                 0.976 
                 CDBox 
                 134 
               
               
                 U44 
                 −1.07 
                 1.28887E−12 
                 0.981 
                 CDBox 
                 134 
               
               
                 miR-139-5p 
                 −2.70 
                 2.74261E−12 
                 0.940 
                 miRNA 
                 80 
               
               
                 miR-1826 
                 −0.71 
                 2.83317E−12 
                 0.958 
                 miRNA 
                 137 
               
               
                 ENSG00000199435 
                 1.38 
                 2.88249E−12 
                 0.954 
                 snoRNA 
                 63 
               
               
                 miR-26a 
                 0.89 
                 2.88328E−12 
                 0.960 
                 miRNA 
                 136 
               
               
                 U58B_x 
                 −1.47 
                 3.60008E−12 
                 0.940 
                 CDBox 
                 132 
               
               
                 HBII-180A_x 
                 −1.43 
                 4.25614E−12 
                 0.992 
                 CDBox 
                 123 
               
               
                 14qll-12_x 
                 −2.09 
                 4.30741E−12 
                 0.949 
                 CDBox 
                 85 
               
               
                 miR-509-3-5p 
                 3.46 
                 5.53398E−12 
                 0.953 
                 miRNA 
                 107 
               
               
                 U55_x 
                 −1.35 
                  6.7836E−12 
                 0.996 
                 CDBox 
                 135 
               
               
                 miR-149-star 
                 0.93 
                  9.6022E−12 
                 0.940 
                 miRNA 
                 135 
               
               
                 HBII-276 
                 −1.41 
                 1.19464E−11 
                 0.964 
                 CDBox 
                 132 
               
               
                 miR-23b 
                 −0.75 
                 1.72105E−11 
                 0.968 
                 miRNA 
                 137 
               
               
                 miR-15b 
                 −2.42 
                  1.7823E−11 
                 0.968 
                 miRNA 
                 125 
               
               
                 miR-921 
                 1.73 
                 1.93285E−11 
                 0.954 
                 miRNA 
                 49 
               
               
                 miR-191 
                 −0.85 
                  2.0881E−11 
                 0.972 
                 miRNA 
                 135 
               
               
                 U103_s 
                 −1.60 
                 2.57483E−11 
                 0.943 
                 CDBox 
                 111 
               
               
                 miR-486-5p 
                 −3.27 
                 2.90873E−11 
                 0.942 
                 miRNA 
                 65 
               
               
                 U27 
                 −1.61 
                 3.28995E−11 
                 0.960 
                 CDBox 
                 134 
               
               
                 miR-1202 
                 2.18 
                 4.44562E−11 
                 0.945 
                 miRNA 
                 57 
               
               
                 U49A 
                 1.25 
                 4.51441E−11 
                 0.970 
                 CDBox 
                 135 
               
               
                 ACA24_x 
                 −1.50 
                 5.61856E−11 
                 0.922 
                 HAcaBox 
                 98 
               
               
                 HBII-382_s 
                 1.30 
                 5.89071E−11 
                 0.953 
                 scaRna 
                 126 
               
               
                 U52 
                 −0.92 
                 1.05668E−10 
                 0.976 
                 CDBox 
                 135 
               
               
                 miR-99b 
                 −1.84 
                 1.06853E−10 
                 0.988 
                 miRNA 
                 129 
               
               
                 U38A 
                 0.84 
                 1.30902E−10 
                 0.936 
                 CDBox 
                 134 
               
               
                 U46 
                 −1.44 
                 1.33613E−10 
                 0.965 
                 CDBox 
                 133 
               
               
                 U57 
                 −1.10 
                 1.66897E−10 
                 0.967 
                 CDBox 
                 135 
               
               
                 U3-2_s 
                 0.70 
                 1.79537E−10 
                 0.925 
                 CDBox 
                 135 
               
               
                 14qll-26_x 
                 −1.60 
                 1.92804E−10 
                 0.922 
                 CDBox 
                 41 
               
               
                 ENSG00000207098_x 
                 1.21 
                 2.17044E−10 
                 0.956 
                 snoRNA 
                 86 
               
               
                 miR-320c 
                 −0.84 
                 2.33286E−10 
                 0.994 
                 miRNA 
                 135 
               
               
                 miR-199a-5p 
                 −2.53 
                 2.85196E−10 
                 0.994 
                 miRNA 
                 129 
               
               
                 HBII-180C 
                 −1.55 
                 3.80017E−10 
                 0.924 
                 CDBox 
                 115 
               
               
                 U63 
                 0.94 
                 4.46838E−10 
                 0.945 
                 CDBox 
                 135 
               
               
                 HBII-436 
                 −1.22 
                 4.98357E−10 
                 0.929 
                 CDBox 
                 102 
               
               
                 U81_x 
                 −1.25 
                 6.27975E−10 
                 0.928 
                 CDBox 
                 120 
               
               
                 HBII-419 
                 1.10 
                 6.92884E−10 
                 0.932 
                 CDBox 
                 133 
               
               
                 U17b 
                 −1.32 
                 8.76094E−10 
                 0.996 
                 HAcaBox 
                 135 
               
               
                 miR-506 
                 2.11 
                 1.46252E−09 
                 0.931 
                 miRNA 
                 76 
               
               
                 miR-1268 
                 1.02 
                 1.54826E−09 
                 0.909 
                 miRNA 
                 133 
               
               
                 miR-671-5p 
                 1.38 
                 1.60795E−09 
                 0.914 
                 miRNA 
                 69 
               
               
                 miR-339-5p 
                 −2.03 
                 1.92249E−09 
                 0.907 
                 miRNA 
                 75 
               
               
                 ACA48_x 
                 −1.25 
                 1.98889E−09 
                 0.945 
                 HAcaBox 
                 130 
               
               
                 14qll-21_x 
                 −1.20 
                 2.04198E−09 
                 0.907 
                 CDBox 
                 51 
               
               
                 ENSG00000202093_x 
                 −1.37 
                 2.13676E−09 
                 0.902 
                 snoRNA 
                 125 
               
               
                 U73a 
                 −0.76 
                 3.11274E−09 
                 0.924 
                 CDBox 
                 135 
               
               
                 miR-1228-star 
                 1.09 
                 3.32612E−09 
                 0.925 
                 miRNA 
                 134 
               
               
                 miR-193b-star 
                 −1.99 
                 3.54373E−09 
                 0.925 
                 miRNA 
                 77 
               
               
                 miR-145 
                 −2.87 
                 3.67076E−09 
                 0.986 
                 miRNA 
                 135 
               
               
                 miR-1207-5p 
                 0.91 
                 3.82606E−09 
                 0.936 
                 miRNA 
                 133 
               
               
                 miR-23a 
                 −0.62 
                 4.38608E−09 
                 0.950 
                 miRNA 
                 137 
               
               
                 14qll-3 
                 −1.37 
                 5.04275E−09 
                 0.938 
                 CDBox 
                 95 
               
               
                 U56 
                 0.82 
                 5.34057E−09 
                 0.910 
                 CDBox 
                 135 
               
               
                 miR-320a 
                 −0.50 
                 5.87182E−09 
                 0.917 
                 miRNA 
                 135 
               
               
                 HBII-289 
                 −0.82 
                 6.53343E−09 
                 0.975 
                 CDBox 
                 135 
               
               
                 mgU6-53B 
                 1.17 
                 6.66322E−09 
                 0.946 
                 CDBox 
                 99 
               
               
                 miR-222 
                 −1.42 
                 6.66965E−09 
                 1.000 
                 miRNA 
                 135 
               
               
                 miR-135a-star 
                 1.78 
                 6.83122E−09 
                 0.914 
                 miRNA 
                 44 
               
               
                 HBII-142 
                 0.50 
                 6.91032E−09 
                 0.911 
                 CDBox 
                 135 
               
               
                 14qll-12 
                 −1.90 
                  7.3584E−09 
                 0.896 
                 CDBox 
                 58 
               
               
                 ACA23 
                 −1.07 
                 9.00722E−09 
                 0.922 
                 HAcaBox 
                 113 
               
               
                 miR-1224-5p 
                 2.00 
                 9.14764E−09 
                 0.902 
                 miRNA 
                 78 
               
               
                 U17b_x 
                 −0.78 
                 9.16941E−09 
                 0.917 
                 HAcaBox 
                 135 
               
               
                 U41 
                 −0.89 
                  1.0052E−08 
                 0.928 
                 CDBox 
                 135 
               
               
                 U55 
                 −1.40 
                 1.07645E−08 
                 0.988 
                 CDBox 
                 135 
               
               
                 U101 
                 1.01 
                 1.22325E−08 
                 0.911 
                 CDBox 
                 134 
               
               
                 miR-193a-5p 
                 −2.09 
                 1.43928E−08 
                 0.954 
                 miRNA 
                 121 
               
               
                 miR-720 
                 0.87 
                 1.45059E−08 
                 0.918 
                 miRNA 
                 137 
               
               
                 miR-205 
                 −1.04 
                 1.53388E−08 
                 0.956 
                 miRNA 
                 135 
               
               
                 mgh28S-2411 
                 −0.86 
                 1.81685E−08 
                 0.965 
                 CDBox 
                 135 
               
               
                 miR-1300 
                 1.84 
                 1.83075E−08 
                 0.911 
                 miRNA 
                 32 
               
               
                 miR-22 
                 −2.31 
                 1.87992E−08 
                 0.943 
                 miRNA 
                 120 
               
               
                 ACA40_x 
                 −1.14 
                 1.89306E−08 
                 0.935 
                 HAcaBox 
                 134 
               
               
                 miR-92a 
                 −1.48 
                 1.96388E−08 
                 1.000 
                 miRNA 
                 136 
               
               
                 14qll-28_x 
                 −1.20 
                 2.08991E−08 
                 0.911 
                 CDBox 
                 51 
               
               
                 U23 
                 −0.93 
                 2.35134E−08 
                 0.913 
                 HAcaBox 
                 127 
               
               
                 snR38C 
                 0.62 
                 2.69211E−08 
                 0.902 
                 CDBox 
                 134 
               
               
                 miR-939 
                 1.33 
                  2.8172E−08 
                 0.947 
                 miRNA 
                 57 
               
               
                 miR-214 
                 −0.88 
                 2.85157E−08 
                 0.921 
                 miRNA 
                 135 
               
               
                 U94 
                 1.03 
                 3.14773E−08 
                 0.898 
                 CDBox 
                 109 
               
               
                 miR-510 
                 2.20 
                 3.61106E−08 
                 0.899 
                 miRNA 
                 82 
               
               
                 miR-181b 
                 −1.85 
                 5.28195E−08 
                 0.961 
                 miRNA 
                 130 
               
               
                 U15B 
                 −1.06 
                 5.57521E−08 
                 0.898 
                 CDBox 
                 129 
               
               
                 gi555853_copy5 
                 −0.51 
                 7.13438E−08 
                 0.910 
                 5.8s rRNA 
                 137 
               
               
                 miR-432 
                 −1.83 
                 7.49361E−08 
                 0.902 
                 miRNA 
                 51 
               
               
                 HBII-142_x 
                 0.47 
                 8.53374E−08 
                 0.886 
                 CDBox 
                 135 
               
               
                 snR38B 
                 1.11 
                 8.92959E−08 
                 0.899 
                 CDBox 
                 126 
               
               
                 ACA36_x 
                 1.12 
                 9.60154E−08 
                 0.885 
                 HAcaBox 
                 88 
               
               
                 miR-320d 
                 −1.66 
                 1.03365E−07 
                 0.997 
                 miRNA 
                 134 
               
               
                 hsa-let-7i 
                 1.72 
                 1.07923E−07 
                 0.947 
                 miRNA 
                 135 
               
               
                 miR-125b-2-star 
                 −1.66 
                 1.09644E−07 
                 0.881 
                 miRNA 
                 58 
               
               
                 miR-572 
                 1.35 
                 1.13617E−07 
                 0.906 
                 miRNA 
                 116 
               
               
                 U102 
                 0.87 
                  1.2385E−07 
                 0.874 
                 CDBox 
                 124 
               
               
                 gi555853_copy8 
                 −0.51 
                 1.25699E−07 
                 0.903 
                 5.8s rRNA 
                 137 
               
               
                 miR-371-5p 
                 1.93 
                 1.26302E−07 
                 0.886 
                 miRNA 
                 64 
               
               
                 miR-125b 
                 −0.97 
                 1.35947E−07 
                 0.988 
                 miRNA 
                 135 
               
               
                 ENSG00000201619 
                 1.40 
                  1.4629E−07 
                 0.892 
                 snoRNA 
                 62 
               
               
                 U78_x 
                 1.11 
                 1.62568E−07 
                 0.921 
                 CDBox 
                 134 
               
               
                 ENSG00000212397 
                 0.90 
                 1.65415E−07 
                 0.898 
                 snoRNA 
                 134 
               
               
                 ENSG00000212182 
                 1.09 
                 1.80486E−07 
                 0.884 
                 snoRNA 
                 59 
               
               
                 hsa-let-7f 
                 1.99 
                  2.0615E−07 
                 0.909 
                 miRNA 
                 130 
               
               
                 HBII-55 
                 −0.82 
                 2.17315E−07 
                 0.940 
                 CDBox 
                 135 
               
               
                 gi555853_copy2 
                 −0.49 
                 2.37022E−07 
                 0.893 
                 5.8s rRNA 
                 137 
               
               
                 ACA15_s 
                 −1.09 
                 2.48572E−07 
                 0.875 
                 HAcaBox 
                 116 
               
               
                 U28_x 
                 −0.90 
                  2.5645E−07 
                 0.896 
                 CDBox 
                 133 
               
               
                 gi555853_copy0 
                 −0.50 
                 2.65495E−07 
                 0.889 
                 5.8s rRNA 
                 137 
               
               
                 gi555853_copy4 
                 −0.48 
                 2.85254E−07 
                 0.898 
                 5.8s rRNA 
                 137 
               
               
                 ENSG00000201816 
                 0.83 
                 3.19058E−07 
                 0.878 
                 snoRNA 
                 49 
               
               
                 gi555853_copy1 
                 −0.48 
                 3.26181E−07 
                 0.886 
                 5.8s rRNA 
                 137 
               
               
                 U60 
                 −0.97 
                 3.26261E−07 
                 0.871 
                 CDBox 
                 103 
               
               
                 gi555853_copy6 
                 −0.47 
                 3.40126E−07 
                 0.900 
                 5.8s rRNA 
                 137 
               
               
                 miR-132 
                 −1.77 
                 3.42625E−07 
                 0.870 
                 miRNA 
                 96 
               
               
                 U68_x 
                 −1.05 
                 3.51611E−07 
                 0.910 
                 HAcaBox 
                 133 
               
               
                 gi555853_copy7 
                 −0.48 
                 4.04469E−07 
                 0.892 
                 5.8s rRNA 
                 137 
               
               
                 ENSG00000201660 
                 1.12 
                 4.27296E−07 
                 0.896 
                 snoRNA 
                 132 
               
               
                 miR-150-star 
                 1.63 
                 4.51511E−07 
                 0.874 
                 miRNA 
                 48 
               
               
                 miR-665 
                 1.55 
                 5.14634E−07 
                 0.871 
                 miRNA 
                 47 
               
               
                 miR-150 
                 −1.60 
                  6.5278E−07 
                 0.884 
                 miRNA 
                 117 
               
               
                 miR-210 
                 −1.24 
                 6.58793E−07 
                 0.911 
                 miRNA 
                 130 
               
               
                 miR-664-star 
                 1.38 
                 6.79224E−07 
                 0.864 
                 miRNA 
                 96 
               
               
                 U49A_x 
                 1.03 
                 7.09035E−07 
                 0.911 
                 CDBox 
                 135 
               
               
                 miR-30c 
                 −1.75 
                 7.10279E−07 
                 0.911 
                 miRNA 
                 125 
               
               
                 ENSG00000212458 
                 0.92 
                 7.71477E−07 
                 0.871 
                 snoRNA 
                 95 
               
               
                 ENSG00000200879 
                 −1.08 
                 7.74449E−07 
                 0.868 
                 snoRNA 
                 120 
               
               
                 hsa-let-7d 
                 0.39 
                 8.11829E−07 
                 0.886 
                 miRNA 
                 135 
               
               
                 14qll-1_x 
                 −1.24 
                 9.36723E−07 
                 0.899 
                 CDBox 
                 131 
               
               
                 14qll-26 
                 −1.14 
                 1.46915E−06 
                 0.827 
                 CDBox 
                 32 
               
               
                 hsa-let-7a 
                 0.51 
                 1.65358E−06 
                 0.841 
                 miRNA 
                 136 
               
               
                 U28 
                 −0.75 
                 1.71566E−06 
                 0.874 
                 CDBox 
                 135 
               
               
                 U68 
                 −0.88 
                 1.83186E−06 
                 0.892 
                 HAcaBox 
                 130 
               
               
                 HBII-180C_x 
                 −0.95 
                 2.06263E−06 
                 0.882 
                 CDBox 
                 131 
               
               
                 gi555853_copy3 
                 −0.45 
                 2.14632E−06 
                 0.863 
                 5.8s rRNA 
                 137 
               
               
                 miR-2001b-star 
                 −1.70 
                 2.20935E−06 
                 0.861 
                 miRNA 
                 74 
               
               
                 miR-1234 
                 1.03 
                  2.2168E−06 
                 0.861 
                 miRNA 
                 68 
               
               
                 U95 
                 −0.36 
                 2.37891E−06 
                 0.852 
                 CDBox 
                 135 
               
               
                 U46_x 
                 −0.98 
                 2.42909E−06 
                 0.873 
                 CDBox 
                 131 
               
               
                 spike_in-control-21 
                 0.74 
                 2.57369E−06 
                 0.827 
                 Oligonucleotide 
                 42 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 miR-1248 
                 1.03 
                 3.51695E−06 
                 0.874 
                 miRNA 
                 39 
               
               
                 U36A 
                 −0.61 
                 3.61256E−06 
                 0.882 
                 CDBox 
                 132 
               
               
                 U13 
                 0.50 
                 4.36581E−06 
                 0.834 
                 CDBox 
                 135 
               
               
                 miR-92a-2-star 
                 1.02 
                 4.99387E−06 
                 0.859 
                 miRNA 
                 46 
               
               
                 miR-663 
                 0.77 
                 5.24563E−06 
                 0.859 
                 miRNA 
                 134 
               
               
                 miR-409-3p 
                 −1.42 
                 5.63777E−06 
                 0.837 
                 miRNA 
                 92 
               
               
                 U34 
                 −0.68 
                 5.88695E−06 
                 0.852 
                 CDBox 
                 137 
               
               
                 U25 
                 −0.71 
                 6.25404E−06 
                 0.896 
                 CDBox 
                 135 
               
               
                 U32A_x 
                 −0.75 
                 6.51615E−06 
                 0.882 
                 CDBox 
                 136 
               
               
                 U96a_x 
                 0.68 
                 6.70342E−06 
                 0.849 
                 CDBox 
                 134 
               
               
                 gi555853_copy9 
                 −0.42 
                  7.0704E−06 
                 0.863 
                 5.8s rRNA 
                 137 
               
               
                 ENSG00000199411_x 
                 0.77 
                 7.82781E−06 
                 0.846 
                 snoRNA 
                 132 
               
               
                 spike_in-control-7 
                 1.00 
                 7.92241E−06 
                 0.848 
                 Oligonucleotide 
                 54 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 miR-198 
                 1.24 
                 8.11341E−06 
                 0.846 
                 miRNA 
                 31 
               
               
                 14ql-9_x 
                 −0.81 
                 8.29164E−06 
                 0.820 
                 CDBox 
                 34 
               
               
                 miR-425 
                 −1.72 
                 8.48515E−06 
                 0.846 
                 miRNA 
                 114 
               
               
                 HBII-82 
                 0.77 
                 8.59528E−06 
                 0.839 
                 CDBox 
                 90 
               
               
                 miR-1180 
                 −1.22 
                 9.75327E−06 
                 0.828 
                 miRNA 
                 37 
               
               
                 ENSG00000206637_x 
                 0.74 
                 1.15035E−05 
                 0.850 
                 snoRNA 
                 59 
               
               
                 miR-502-3p 
                 −1.41 
                 1.18784E−05 
                 0.831 
                 miRNA 
                 110 
               
               
                 4qll-22_x 
                 −0.97 
                 1.49319E−05 
                 0.792 
                 CDBox 
                 50 
               
               
                 ACA18_x 
                 −0.72 
                 1.77602E−05 
                 0.837 
                 HAcaBox 
                 135 
               
               
                 miR-1274b 
                 1.66 
                 1.82625E−05 
                 0.813 
                 miRNA 
                 112 
               
               
                 HBII-99 
                 −0.94 
                 1.84996E−05 
                 0.837 
                 CDBox 
                 122 
               
               
                 U35B 
                 0.78 
                 1.93006E−05 
                 0.837 
                 CDBox 
                 128 
               
               
                 ACA20_x 
                 −0.84 
                 2.02452E−05 
                 0.882 
                 HAcaBox 
                 134 
               
               
                 miR-143 
                 −2.21 
                  2.0661E−05 
                 0.864 
                 miRNA 
                 126 
               
               
                 ENSG00000200652 
                 0.81 
                 2.09666E−05 
                 0.824 
                 snoRNA 
                 40 
               
               
                 HBII-202 
                 −0.59 
                 2.32067E−05 
                 0.886 
                 CDBox 
                 135 
               
               
                 miR-708 
                 −1.81 
                 2.34758E−05 
                 0.816 
                 miRNA 
                 102 
               
               
                 miR-182 
                 −1.64 
                 2.56273E−05 
                 0.791 
                 miRNA 
                 113 
               
               
                 miR-345 
                 −1.28 
                  3.1705E−05 
                 0.816 
                 miRNA 
                 75 
               
               
                 ACA62 
                 0.82 
                 3.35475E−05 
                 0.817 
                 HAcaBox 
                 88 
               
               
                 miR-1273 
                 1.12 
                 3.38999E−05 
                 0.827 
                 miRNA 
                 47 
               
               
                 ACA24_s 
                 −1.10 
                 3.44078E−05 
                 0.823 
                 HAcaBox 
                 128 
               
               
                 miR-1272 
                 0.97 
                 3.51756E−05 
                 0.820 
                 miRNA 
                 67 
               
               
                 14ql-4_x 
                 −1.11 
                 3.64993E−05 
                 0.796 
                 CDBox 
                 51 
               
               
                 14qll-9_x 
                 −0.89 
                 3.72476E−05 
                 0.820 
                 CDBox 
                 36 
               
               
                 U106 
                 −0.62 
                 3.79813E−05 
                 0.817 
                 CDBox 
                 112 
               
               
                 miR-1285 
                 1.22 
                 4.16925E−05 
                 0.796 
                 miRNA 
                 54 
               
               
                 ENSG00000200492 
                 0.77 
                 4.61258E−05 
                 0.838 
                 snoRNA 
                 60 
               
               
                 ENSG00000202327 
                 0.77 
                 4.61736E−05 
                 0.812 
                 snoRNA 
                 54 
               
               
                 miR-126 
                 1.24 
                 4.86215E−05 
                 0.845 
                 miRNA 
                 133 
               
               
                 U49B_x 
                 0.62 
                 4.92521E−05 
                 0.795 
                 CDBox 
                 122 
               
               
                 ENSG00000207016_x 
                 0.74 
                  5.4547E−05 
                 0.794 
                 snoRNA 
                 48 
               
               
                 HBII-52-25_x 
                 0.68 
                 5.50793E−05 
                 0.827 
                 CDBox 
                 57 
               
               
                 U49B_s 
                 0.90 
                 5.94839E−05 
                 0.848 
                 CDBox 
                 129 
               
               
                 U53 
                 −0.97 
                 6.07982E−05 
                 0.809 
                 CDBox 
                 126 
               
               
                 miR-1246 
                 1.19 
                 7.10577E−05 
                 0.799 
                 miRNA 
                 131 
               
               
                 ENSG00000212615_x 
                 −0.67 
                 7.76933E−05 
                 0.798 
                 snoRNA 
                 102 
               
               
                 miR-575 
                 1.09 
                 8.31223E−05 
                 0.794 
                 miRNA 
                 41 
               
               
                 snR38A 
                 0.69 
                 8.40239E−05 
                 0.807 
                 CDBox 
                 130 
               
               
                 14ql-7 
                 −0.78 
                 8.70579E−05 
                 0.825 
                 CDBox 
                 51 
               
               
                 miR-29b-2-star 
                 0.86 
                  8.9139E−05 
                 0.802 
                 miRNA 
                 126 
               
               
                 U80 
                 −0.60 
                 9.75089E−05 
                 0.825 
                 CDBox 
                 132 
               
               
                 miR-1225-5p 
                 1.04 
                 9.99249E−05 
                 0.830 
                 miRNA 
                 90 
               
               
                 miR-92b-star 
                 0.94 
                 0.000102055 
                 0.809 
                 miRNA 
                 111 
               
               
                 ACA53 
                 −0.61 
                 0.000103826 
                 0.814 
                 HAcaBox 
                 105 
               
               
                 ACA55 
                 0.79 
                 0.000105025 
                 0.805 
                 HAcaBox 
                 85 
               
               
                 U51 
                 0.58 
                 0.000112048 
                 0.802 
                 CDBox 
                 134 
               
               
                 U108_x 
                 0.55 
                 0.000112181 
                 0.798 
                 HAcaBox 
                 87 
               
               
                 U48 
                 −1.07 
                 0.000118819 
                 0.875 
                 CDBox 
                 129 
               
               
                 miR-221 
                 −1.06 
                 0.00013613 
                 0.911 
                 miRNA 
                 135 
               
               
                 HBII-13 
                 0.74 
                 0.000139215 
                 0.798 
                 CDBox 
                 52 
               
               
                 miR-19b 
                 1.53 
                 0.0001409 
                 0.795 
                 miRNA 
                 124 
               
               
                 ACA3-2 
                 −0.76 
                 0.000143105 
                 0.819 
                 HAcaBox 
                 135 
               
               
                 HBII-85-23_x 
                 −0.73 
                 0.000143482 
                 0.787 
                 CDBox 
                 58 
               
               
                 14ql-8 
                 −0.75 
                 0.000167587 
                 0.802 
                 CDBox 
                 61 
               
               
                 miR-138-1-star 
                 0.88 
                 0.000190948 
                 0.798 
                 miRNA 
                 91 
               
               
                 spike_in-control-28 
                 0.63 
                 0.000200982 
                 0.802 
                 Oligonucleotide 
                 76 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 miR-500-star 
                 −1.36 
                 0.000204792 
                 0.803 
                 miRNA 
                 97 
               
               
                 ENSG00000212423_x 
                 0.75 
                 0.000232739 
                 0.789 
                 snoRNA 
                 115 
               
               
                 ENSG00000201348 
                 0.50 
                 0.000243216 
                 0.778 
                 snoRNA 
                 94 
               
               
                 miR-1275 
                 1.09 
                 0.000244874 
                 0.821 
                 miRNA 
                 127 
               
               
                 miR-30a-star 
                 −1.14 
                 0.000247652 
                 0.758 
                 miRNA 
                 64 
               
               
                 miR-93-star 
                 −1.16 
                 0.000248272 
                 0.777 
                 miRNA 
                 48 
               
               
                 ACA25 
                 −0.64 
                 0.000248534 
                 0.802 
                 HAcaBox 
                 73 
               
               
                 U50 
                 −0.73 
                 0.000255129 
                 0.853 
                 CDBox 
                 134 
               
               
                 14qll-1 
                 −1.00 
                 0.000255606 
                 0.807 
                 CDBox 
                 128 
               
               
                 U14B_x 
                 −0.79 
                 0.000258746 
                 0.788 
                 CDBox 
                 53 
               
               
                 miR-638 
                 0.35 
                 0.000261809 
                 0.823 
                 miRNA 
                 135 
               
               
                 U51_x 
                 0.57 
                 0.000299812 
                 0.820 
                 CDBox 
                 130 
               
               
                 miR-99a 
                 −0.87 
                 0.000321188 
                 0.832 
                 miRNA 
                 135 
               
               
                 miR-491-5p 
                 1.04 
                 0.000341416 
                 0.756 
                 miRNA 
                 92 
               
               
                 miR-508-3p 
                 1.23 
                 0.000350578 
                 0.778 
                 miRNA 
                 74 
               
               
                 miR-508-5p 
                 1.02 
                 0.000377448 
                 0.783 
                 miRNA 
                 92 
               
               
                 miR-641 
                 0.88 
                 0.000391728 
                 0.774 
                 miRNA 
                 39 
               
               
                 miR-885-5p 
                 −1.23 
                 0.00039874 
                 0.801 
                 miRNA 
                 100 
               
               
                 miR-497 
                 −1.48 
                 0.00041742 
                 0.780 
                 miRNA 
                 104 
               
               
                 U31_x 
                 −0.65 
                 0.000420569 
                 0.795 
                 CDBox 
                 135 
               
               
                 miR-498 
                 0.85 
                 0.000424439 
                 0.787 
                 miRNA 
                 56 
               
               
                 miR-20a 
                 1.21 
                 0.000429079 
                 0.807 
                 miRNA 
                 134 
               
               
                 miR-509-5p 
                 1.35 
                 0.000476275 
                 0.776 
                 miRNA 
                 81 
               
               
                 miR-331-3p 
                 −1.11 
                 0.000481237 
                 0.777 
                 miRNA 
                 64 
               
               
                 HBII-295 
                 0.53 
                 0.000526137 
                 0.785 
                 CDBox 
                 128 
               
               
                 ACA27_x 
                 −0.45 
                 0.000528795 
                 0.778 
                 HAcaBox 
                 130 
               
               
                 miR-155 
                 −1.29 
                 0.000530869 
                 0.751 
                 miRNA 
                 123 
               
               
                 miR-652 
                 −1.30 
                 0.000593692 
                 0.774 
                 miRNA 
                 119 
               
               
                 ENSG00000208308_x 
                 −0.58 
                 0.000595144 
                 0.758 
                 snoRNA 
                 129 
               
               
                 miR-1281 
                 0.98 
                 0.000606821 
                 0.766 
                 miRNA 
                 137 
               
               
                 E3_x 
                 −0.53 
                 0.000619964 
                 0.819 
                 HAcaBox 
                 135 
               
               
                 miR-27a-star 
                 −0.88 
                 0.000656179 
                 0.773 
                 miRNA 
                 40 
               
               
                 miR-92b 
                 −0.90 
                 0.0006705 
                 0.773 
                 miRNA 
                 91 
               
               
                 miR-570 
                 0.93 
                 0.000670846 
                 0.776 
                 miRNA 
                 88 
               
               
                 miR-181a-2-star 
                 −1.19 
                 0.000676824 
                 0.753 
                 miRNA 
                 99 
               
               
                 miR-1185 
                 0.76 
                 0.000680122 
                 0.752 
                 miRNA 
                 42 
               
               
                 ACA33 
                 0.57 
                 0.000718022 
                 0.780 
                 HAcaBox 
                 133 
               
               
                 ENSG00000212401 
                 0.57 
                 0.000773614 
                 0.799 
                 snoRNA 
                 41 
               
               
                 U20 
                 −0.51 
                 0.000816772 
                 0.758 
                 CDBox 
                 117 
               
               
                 U19 
                 0.84 
                 0.00082895 
                 0.759 
                 HAcaBox 
                 118 
               
               
                 miR-203 
                 0.69 
                 0.000839225 
                 0.878 
                 miRNA 
                 132 
               
               
                 ENSG00000212508 
                 0.83 
                 0.000873066 
                 0.801 
                 snoRNA 
                 122 
               
               
                 ACA54 
                 −0.54 
                 0.000880465 
                 0.817 
                 HAcaBox 
                 133 
               
               
                 ACA9_x 
                 −0.68 
                 0.001025389 
                 0.752 
                 HAcaBox 
                 77 
               
               
                 14ql-1 
                 0.61 
                 0.001136212 
                 0.729 
                 CDBox 
                 70 
               
               
                 miR-940 
                 0.87 
                 0.001164857 
                 0.737 
                 miRNA 
                 53 
               
               
                 U14B 
                 −0.69 
                 0.001178164 
                 0.738 
                 CDBox 
                 51 
               
               
                 mgU6-77 
                 −0.62 
                 0.001238112 
                 0.760 
                 CDBox 
                 129 
               
               
                 spike_in-control-30 
                 0.54 
                 0.001290622 
                 0.753 
                 Oligonucleotide 
                 53 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 miR-629-star 
                 −0.92 
                 0.00138462 
                 0.756 
                 miRNA 
                 46 
               
               
                 ACA49 
                 0.56 
                 0.001394857 
                 0.791 
                 HAcaBox 
                 127 
               
               
                 miR-483-5p 
                 0.96 
                 0.001461794 
                 0.738 
                 miRNA 
                 48 
               
               
                 ENSG00000212214_x 
                 0.51 
                 0.001505107 
                 0.748 
                 snoRNA 
                 88 
               
               
                 miR-574-5p 
                 −0.99 
                 0.00156768 
                 0.766 
                 miRNA 
                 103 
               
               
                 miR-28-3p 
                 −1.29 
                 0.001658961 
                 0.747 
                 miRNA 
                 94 
               
               
                 U36C 
                 0.34 
                 0.001694833 
                 0.849 
                 CDBox 
                 135 
               
               
                 miR-1288 
                 0.73 
                 0.001697813 
                 0.738 
                 miRNA 
                 43 
               
               
                 ACA37_x 
                 −0.59 
                 0.001721183 
                 0.742 
                 HAcaBox 
                 84 
               
               
                 miR-1280 
                 −0.72 
                 0.001869035 
                 0.777 
                 miRNA 
                 134 
               
               
                 miR-487b 
                 −0.96 
                 0.001988497 
                 0.731 
                 miRNA 
                 58 
               
               
                 miR-532-3p 
                 −0.80 
                 0.00199381 
                 0.766 
                 miRNA 
                 95 
               
               
                 miR-346 
                 −0.91 
                 0.0020172 
                 0.749 
                 miRNA 
                 55 
               
               
                 miR-27b-star 
                 −1.04 
                 0.00209985 
                 0.742 
                 miRNA 
                 51 
               
               
                 ACA32 
                 0.34 
                 0.002161552 
                 0.785 
                 HAcaBox 
                 135 
               
               
                 ENSG00000212206_x 
                 0.58 
                 0.002337296 
                 0.740 
                 snoRNA 
                 74 
               
               
                 ACA51_x 
                 0.43 
                 0.002449336 
                 0.742 
                 HAcaBox 
                 135 
               
               
                 ENSG00000201847_x 
                 −0.53 
                 0.002461896 
                 0.729 
                 snoRNA 
                 48 
               
               
                 miR-513c 
                 1.13 
                 0.002510473 
                 0.749 
                 miRNA 
                 73 
               
               
                 miR-455-3p 
                 −0.62 
                 0.00258213 
                 0.785 
                 miRNA 
                 136 
               
               
                 miR-188-5p 
                 −0.90 
                 0.002825937 
                 0.730 
                 miRNA 
                 51 
               
               
                 ENSG00000207027 
                 0.48 
                 0.002903474 
                 0.704 
                 snoRNA 
                 36 
               
               
                 ENSG00000202498_x 
                 0.56 
                 0.002908782 
                 0.735 
                 snoRNA 
                 137 
               
               
                 HBII-85-26 
                 0.66 
                 0.003001767 
                 0.741 
                 CDBox 
                 136 
               
               
                 miR-337-3p 
                 0.80 
                 0.003015225 
                 0.740 
                 miRNA 
                 94 
               
               
                 U35A 
                 −0.47 
                 0.003204612 
                 0.723 
                 CDBox 
                 135 
               
               
                 miR-106b-star 
                 −1.10 
                 0.003210131 
                 0.717 
                 miRNA 
                 72 
               
               
                 ENSG00000202216 
                 0.55 
                 0.003292357 
                 0.758 
                 snoRNA 
                 53 
               
               
                 HBII-316 
                 0.63 
                 0.003333247 
                 0.705 
                 CDBox 
                 130 
               
               
                 miR-31 
                 −1.63 
                 0.003410838 
                 0.720 
                 miRNA 
                 103 
               
               
                 U22 
                 0.43 
                 0.003510102 
                 0.699 
                 CDBox 
                 135 
               
               
                 miR-34a-star 
                 0.77 
                 0.003765001 
                 0.715 
                 miRNA 
                 39 
               
               
                 HBII-85-6_x 
                 0.62 
                 0.003870576 
                 0.735 
                 CDBox 
                 137 
               
               
                 ACA15_x 
                 −0.50 
                 0.003952375 
                 0.734 
                 HAcaBox 
                 124 
               
               
                 ENSG00000200706_x 
                 0.48 
                 0.00407636 
                 0.762 
                 snoRNA 
                 43 
               
               
                 ENSG00000212627 
                 0.41 
                 0.00409948 
                 0.726 
                 snoRNA 
                 69 
               
               
                 14qll-7 
                 0.59 
                 0.004220389 
                 0.719 
                 CDBox 
                 77 
               
               
                 ENSG00000212432_s 
                 0.52 
                 0.004352612 
                 0.745 
                 snoRNA 
                 75 
               
               
                 miR-214-star 
                 −0.82 
                 0.004514439 
                 0.708 
                 miRNA 
                 47 
               
               
                 miR-106a 
                 0.58 
                 0.004682448 
                 0.724 
                 miRNA 
                 135 
               
               
                 ACA42 
                 −0.54 
                 0.004759271 
                 0.720 
                 HAcaBox 
                 117 
               
               
                 mgU6-53B_x 
                 0.46 
                 0.0048197 
                 0.720 
                 CDBox 
                 121 
               
               
                 ACA19 
                 0.38 
                 0.005153495 
                 0.733 
                 HAcaBox 
                 118 
               
               
                 ACA50 
                 −0.53 
                 0.005300761 
                 0.738 
                 HAcaBox 
                 88 
               
               
                 miR-138 
                 1.07 
                 0.005618175 
                 0.716 
                 miRNA 
                 82 
               
               
                 U47 
                 −0.47 
                 0.005755719 
                 0.729 
                 CDBox 
                 74 
               
               
                 ENSG00000212551 
                 0.48 
                 0.005885716 
                 0.740 
                 snoRNA 
                 73 
               
               
                 miR-769-5p 
                 −0.93 
                 0.005951319 
                 0.687 
                 miRNA 
                 56 
               
               
                 miR-1228 
                 0.82 
                 0.006114287 
                 0.724 
                 miRNA 
                 114 
               
               
                 miR-30b 
                 0.87 
                 0.00634299 
                 0.748 
                 miRNA 
                 128 
               
               
                 ENSG00000212315 
                 0.54 
                 0.006470787 
                 0.752 
                 snoRNA 
                 117 
               
               
                 ENSG00000212538 
                 0.48 
                 0.006558923 
                 0.735 
                 snoRNA 
                 35 
               
               
                 ACA2b 
                 0.39 
                 0.007073038 
                 0.756 
                 HAcaBox 
                 47 
               
               
                 ENSG00000212523_x 
                 0.59 
                 0.007115341 
                 0.716 
                 snoRNA 
                 135 
               
               
                 miR-196a 
                 1.04 
                 0.007138032 
                 0.705 
                 miRNA 
                 77 
               
               
                 HBII-429 
                 0.22 
                 0.007262326 
                 0.720 
                 CDBox 
                 137 
               
               
                 miR-16 
                 0.46 
                 0.007360456 
                 0.749 
                 miRNA 
                 135 
               
               
                 mgh28S-2409 
                 0.27 
                 0.007642791 
                 0.741 
                 CDBox 
                 135 
               
               
                 ENSG00000200307_x 
                 0.54 
                 0.007656242 
                 0.695 
                 snoRNA 
                 50 
               
               
                 U76 
                 −0.27 
                 0.008469646 
                 0.734 
                 CDBox 
                 135 
               
               
                 14qll-17 
                 −0.54 
                 0.008809376 
                 0.698 
                 CDBox 
                 71 
               
               
                 miR-324-3p 
                 −0.67 
                 0.009111464 
                 0.726 
                 miRNA 
                 100 
               
               
                 ACA48 
                 −0.49 
                 0.009193835 
                 0.798 
                 HAcaBox 
                 121 
               
               
                 miR-200c 
                 −0.31 
                 0.009670586 
                 0.625 
                 miRNA 
                 134 
               
               
                 miR-1260 
                 −0.78 
                 0.010502121 
                 0.726 
                 miRNA 
                 120 
               
               
                 miR-130a 
                 −0.94 
                 0.010557093 
                 0.687 
                 miRNA 
                 122 
               
               
                 U17a 
                 −0.52 
                 0.010593144 
                 0.702 
                 HAcaBox 
                 105 
               
               
                 ACA52 
                 −0.45 
                 0.010843862 
                 0.708 
                 HAcaBox 
                 121 
               
               
                 miR-197 
                 −0.79 
                 0.011117393 
                 0.724 
                 miRNA 
                 103 
               
               
                 U66 
                 −0.54 
                 0.011246742 
                 0.705 
                 HAcaBox 
                 119 
               
               
                 ENSG00000202252 
                 −0.30 
                 0.011273976 
                 0.699 
                 snoRNA 
                 135 
               
               
                 ACA61 
                 0.22 
                 0.011490951 
                 0.730 
                 HAcaBox 
                 135 
               
               
                 miR-151-5p 
                 0.56 
                 0.01153084 
                 0.830 
                 miRNA 
                 135 
               
               
                 ACA16_x 
                 −0.47 
                 0.011881173 
                 0.727 
                 HAcaBox 
                 98 
               
               
                 U18C_x 
                 −0.45 
                 0.012073238 
                 0.691 
                 CDBox 
                 74 
               
               
                 U15A 
                 0.45 
                 0.012634875 
                 0.706 
                 CDBox 
                 128 
               
               
                 miR-1274a 
                 0.71 
                 0.012698242 
                 0.684 
                 miRNA 
                 48 
               
               
                 ACA 
                 −0.62 
                 0.01271037 
                 0.691 
                 HAcaBox 
                 82 
               
               
                 U91_s 
                 −0.54 
                 0.012949986 
                 0.733 
                 scaRna 
                 132 
               
               
                 ENSG00000207002 
                 −0.52 
                 0.013212929 
                 0.702 
                 snoRNA 
                 35 
               
               
                 miR-129-3p 
                 −0.54 
                 0.013303701 
                 0.705 
                 miRNA 
                 59 
               
               
                 spike_in-cortrol-17 
                 0.48 
                 0.013556785 
                 0.705 
                 Oligonucleotide 
                 33 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 spike_in-control-2 
                 0.19 
                 0.014699249 
                 0.684 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 ENSG00000212206 
                 0.49 
                 0.014866744 
                 0.670 
                 snoRNA 
                 46 
               
               
                 miR-107 
                 −0.39 
                 0.015131674 
                 0.669 
                 miRNA 
                 135 
               
               
                 ACA63 
                 0.42 
                 0.01519582 
                 0.695 
                 HAcaBox 
                 66 
               
               
                 ENSG00000212553_x 
                 0.45 
                 0.015776333 
                 0.684 
                 snoRNA 
                 60 
               
               
                 U50B 
                 −0.37 
                 0.016500241 
                 0.704 
                 CDBox 
                 135 
               
               
                 ENSG00000207100_x 
                 0.36 
                 0.016825837 
                 0.666 
                 snoRNA 
                 72 
               
               
                 U78_s 
                 −0.55 
                 0.016840855 
                 0.702 
                 CDBox 
                 135 
               
               
                 miR-551b-star 
                 −0.65 
                 0.016907402 
                 0.686 
                 miRNA 
                 61 
               
               
                 ACA47 
                 0.45 
                 0.017340107 
                 0.666 
                 scaRna 
                 77 
               
               
                 U92 
                 0.45 
                 0.017435918 
                 0.760 
                 scaRna 
                 129 
               
               
                 miR-342-5p 
                 −0.78 
                 0.01779742 
                 0.695 
                 miRNA 
                 98 
               
               
                 miR-128 
                 −0.56 
                 0.017820912 
                 0.699 
                 miRNA 
                 44 
               
               
                 U17a_x 
                 −0.46 
                 0.018370462 
                 0.720 
                 HAcaBox 
                 115 
               
               
                 ENSG00000206909_x 
                 0.44 
                 0.018373221 
                 0.706 
                 snoRNA 
                 47 
               
               
                 U30 
                 −0.35 
                 0.018607695 
                 0.729 
                 CDBox 
                 135 
               
               
                 miR-98 
                 −0.57 
                 0.019510485 
                 0.677 
                 miRNA 
                 47 
               
               
                 miR-21-star 
                 0.62 
                 0.019769732 
                 0.686 
                 miRNA 
                 64 
               
               
                 miR-135b-star 
                 0.57 
                 0.0198019 
                 0.704 
                 miRNA 
                 38 
               
               
                 ACA2a 
                 −0.42 
                 0.019986353 
                 0.677 
                 HAcaBox 
                 55 
               
               
                 U88 
                 0.44 
                 0.020351208 
                 0.672 
                 scaRna 
                 69 
               
               
                 HBII-85-29_x 
                 0.39 
                 0.020664805 
                 0.666 
                 CDBox 
                 85 
               
               
                 14qll-19 
                 0.47 
                 0.02089152 
                 0.695 
                 CDBox 
                 43 
               
               
                 U83B 
                 0.19 
                 0.020956657 
                 0.677 
                 CDBox 
                 135 
               
               
                 miR-134 
                 −0.86 
                 0.020986754 
                 0.681 
                 miRNA 
                 61 
               
               
                 U83A 
                 −0.40 
                 0.021328386 
                 0.698 
                 CDBox 
                 127 
               
               
                 ENSG00000202370 
                 0.53 
                 0.02172281 
                 0.651 
                 snoRNA 
                 88 
               
               
                 U75_x 
                 −0.35 
                 0.021862862 
                 0.697 
                 HAcaBox 
                 103 
               
               
                 ACA38 
                 −0.38 
                 0.023364662 
                 0.688 
                 HAcaBox 
                 42 
               
               
                 ENSG00000200394_x 
                 0.42 
                 0.023498919 
                 0.661 
                 snoRNA 
                 115 
               
               
                 miR-1324 
                 0.50 
                 0.023518344 
                 0.684 
                 miRNA 
                 57 
               
               
                 miR-106b 
                 0.85 
                 0.023747964 
                 0.670 
                 miRNA 
                 127 
               
               
                 miR-589-star 
                 0.56 
                 0.023792146 
                 0.675 
                 miRNA 
                 43 
               
               
                 ACA6 
                 −0.36 
                 0.024014637 
                 0.673 
                 HAcaBox 
                 126 
               
               
                 ACA28 
                 0.36 
                 0.02403059 
                 0.687 
                 HAcaBox 
                 120 
               
               
                 ACA57 
                 0.36 
                 0.024679323 
                 0.748 
                 scaRna 
                 135 
               
               
                 HBII-85-4_x 
                 0.36 
                 0.024717662 
                 0.711 
                 CDBox 
                 125 
               
               
                 ENSG00000200897 
                 −0.41 
                 0.024775335 
                 0.662 
                 snoRNA 
                 38 
               
               
                 U16 
                 0.42 
                 0.024969966 
                 0.712 
                 CDBox 
                 131 
               
               
                 ENSG00000200418 
                 −0.41 
                 0.025790882 
                 0.668 
                 snoRNA 
                 31 
               
               
                 U105 
                 −0.31 
                 0.026442326 
                 0.673 
                 CDBox 
                 126 
               
               
                 spike_in-control-34 
                 0.44 
                 0.026481739 
                 0.665 
                 Oligonucleotide 
                 58 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 spike_in-control-23 
                 0.24 
                 0.027477358 
                 0.645 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 miR-196a-star 
                 0.61 
                 0.028243677 
                 0.677 
                 miRNA 
                 37 
               
               
                 HBII-296A 
                 0.33 
                 0.02845867 
                 0.659 
                 CDBox 
                 78 
               
               
                 U82 
                 0.43 
                 0.028925335 
                 0.681 
                 CDBox 
                 131 
               
               
                 ENSG00000212558_x 
                 −0.35 
                 0.029963606 
                 0.662 
                 snoRNA 
                 31 
               
               
                 miR-21 
                 0.67 
                 0.030060673 
                 0.668 
                 miRNA 
                 79 
               
               
                 ACA31 
                 0.37 
                 0.030929941 
                 0.702 
                 HAcaBox 
                 123 
               
               
                 miR-1257 
                 0.55 
                 0.031016296 
                 0.676 
                 miRNA 
                 31 
               
               
                 ACA14b_x 
                 −0.37 
                 0.031018594 
                 0.688 
                 HAcaBox 
                 105 
               
               
                 ACA33_x 
                 0.38 
                 0.033957171 
                 0.687 
                 HAcaBox 
                 129 
               
               
                 miR-199b-3p 
                 0.64 
                 0.034530062 
                 0.737 
                 miRNA 
                 134 
               
               
                 miR-100 
                 −0.61 
                 0.034553062 
                 0.681 
                 miRNA 
                 133 
               
               
                 U64 
                 −0.38 
                 0.034971581 
                 0.633 
                 HAcaBox 
                 101 
               
               
                 miR-10a 
                 −0.67 
                 0.037624151 
                 0.666 
                 miRNA 
                 69 
               
               
                 miR-30e 
                 0.56 
                 0.037770496 
                 0.683 
                 miRNA 
                 57 
               
               
                 miR-199a-3p 
                 0.62 
                 0.038490813 
                 0.704 
                 miRNA 
                 134 
               
               
                 HBII-85-11 
                 −0.37 
                 0.038507691 
                 0.687 
                 CDBox 
                 31 
               
               
                 miR-505-star 
                 −0.62 
                 0.039242017 
                 0.647 
                 miRNA 
                 58 
               
               
                 hsa-let-7f-1-star 
                 0.44 
                 0.042251211 
                 0.658 
                 miRNA 
                 64 
               
               
                 spike_in-control-29 
                 −0.19 
                 0.043064083 
                 0.684 
                 Oligonucleotide 
                 137 
               
               
                   
                   
                   
                   
                 spike-in 
               
               
                   
                   
                   
                   
                 controls 
               
               
                 miR-20b 
                 0.64 
                 0.043397678 
                 0.655 
                 miRNA 
                 99 
               
               
                 ACA5 
                 −0.40 
                 0.044405124 
                 0.693 
                 HAcaBox 
                 85 
               
               
                 miR-628-3p 
                 0.57 
                 0.044447799 
                 0.652 
                 miRNA 
                 38 
               
               
                 HBII-85-8_x 
                 0.40 
                 0.045108021 
                 0.681 
                 CDBox 
                 135 
               
               
                 HBII-85-27_x 
                 0.29 
                 0.045678744 
                 0.651 
                 CDBox 
                 56 
               
               
                 miR-99b-star 
                 0.49 
                 0.049531821 
                 0.651 
                 miRNA 
                 74 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 50 miRNAs most significantly expressed between nevi and normal skin 
               
             
          
           
               
                   
                 Log2- 
                   
                   
                   
                   
               
               
                 Probe Name 
                 FC 
                 P value 
                 AUC 
                 ProbeType 
                 #Sample Detected 
               
               
                   
               
             
          
           
               
                 -14 
                 −3.87 
                  3.6592E−27 
                 1.000 
                 CDBox 
                 56 
               
               
                 14qll-14_x 
                 −3.21 
                  3.815E−24 
                 1.000 
                 CDBox 
                 56 
               
               
                 U74_x 
                 1.46 
                 3.89068E−23 
                 1.000 
                 CDBox 
                 135 
               
               
                 miR-509-3p 
                 5.78 
                 1.67661E−22 
                 0.997 
                 miRNA 
                 116 
               
               
                 miR-768-5p 
                 −1.46 
                 5.23441E−22 
                 1.000 
                 miRNA 
                 135 
               
               
                 ENSG00000199411_s 
                 1.36 
                 1.56796E−21 
                 1.000 
                 snoRNA 
                 135 
               
               
                 Z17B 
                 −1.94 
                 2.89347E−20 
                 0.997 
                 CDBox 
                 117 
               
               
                 miR-149 
                 −3.46 
                 1.55425E−19 
                 1.000 
                 miRNA 
                 100 
               
               
                 miR-125a-5p 
                 −3.44 
                 1.86526E−19 
                 1.000 
                 miRNA 
                 128 
               
               
                 U43 
                 −1.19 
                 3.32514E−19 
                 1.000 
                 CDBox 
                 135 
               
               
                 miR-1308 
                 1.55 
                  8.1184E−19 
                 0.964 
                 miRNA 
                 135 
               
               
                 U59A 
                 1.13 
                 9.99768E−18 
                 0.993 
                 CDBox 
                 135 
               
               
                 miR-146a 
                 4.44 
                 3.16232E−17 
                 0.975 
                 miRNA 
                 129 
               
               
                 miR-513a-5p 
                 3.77 
                 1.05679E−16 
                 0.972 
                 miRNA 
                 105 
               
               
                 ACA20 
                 −1.74 
                 1.56297E−16 
                 0.993 
                 HAcaBox 
                 131 
               
               
                 U43_x 
                 −1.05 
                 1.04459E−15 
                 1.000 
                 CDBox 
                 135 
               
               
                 U44_x 
                 −1.37 
                 5.54123E−15 
                 0.994 
                 CDBox 
                 135 
               
               
                 miR-127-3p 
                 −3.56 
                 7.53549E−15 
                 0.988 
                 miRNA 
                 89 
               
               
                 miR-193b 
                 −2.57 
                 7.81928E−15 
                 1.000 
                 miRNA 
                 130 
               
               
                 U83 
                 −1.25 
                 9.30493E−15 
                 0.993 
                 CDBox 
                 135 
               
               
                 miR-768-3p 
                 0.98 
                 2.39925E−14 
                 0.972 
                 miRNA 
                 137 
               
               
                 U33 
                 −1.02 
                 2.44194E−14 
                 0.997 
                 CDBox 
                 137 
               
               
                 miR-574-3p 
                 −3.05 
                  3.0433E−14 
                 0.974 
                 miRNA 
                 122 
               
               
                 ACA16 
                 −1.86 
                 3.35128E−14 
                 0.968 
                 HAcaBox 
                 47 
               
               
                 14ql-4 
                 −2.43 
                 3.96317E−14 
                 0.979 
                 CDBox 
                 80 
               
               
                 hsa-let-7c 
                 −1.01 
                 4.99111E−14 
                 0.986 
                 miRNA 
                 135 
               
               
                 miR-342-3p 
                 −2.06 
                 1.67074E−13 
                 0.996 
                 miRNA 
                 136 
               
               
                 hsa-let-7b 
                 −1.01 
                 2.25904E−13 
                 0.964 
                 miRNA 
                 136 
               
               
                 miR-423-3p 
                 −2.52 
                 2.97967E−13 
                 1.000 
                 miRNA 
                 107 
               
               
                 HBII-239 
                 −1.41 
                  3.2162E−13 
                 0.975 
                 CDBox 
                 135 
               
               
                 U38B_x 
                 1.52 
                 3.80613E−13 
                 0.968 
                 CDBox 
                 133 
               
               
                 miR-320b 
                 −0.63 
                 6.31909E−13 
                 0.971 
                 miRNA 
                 135 
               
               
                 miR-923 
                 0.93 
                 6.48332E−13 
                 0.990 
                 miRNA 
                 135 
               
               
                 U54 
                 −1.06 
                 1.17197E−12 
                 0.975 
                 CDBox 
                 135 
               
               
                 U38B 
                 1.80 
                 1.18885E−12 
                 0.976 
                 CDBox 
                 134 
               
               
                 U44 
                 −1.07 
                 1.28887E−12 
                 0.981 
                 CDBox 
                 134 
               
               
                 miR-139-5p 
                 −2.70 
                 2.74261E−12 
                 0.940 
                 miRNA 
                 80 
               
               
                 miR-1826 
                 −0.71 
                 2.83317E−12 
                 0.958 
                 miRNA 
                 137 
               
               
                 ENSG00000199435 
                 1.38 
                 2.88249E−12 
                 0.954 
                 snoRNA 
                 63 
               
               
                 miR-26a 
                 0.89 
                 2.88328E−12 
                 0.960 
                 miRNA 
                 136 
               
               
                 U58B_x 
                 −1.47 
                 3.60008E−12 
                 0.940 
                 CDBox 
                 132 
               
               
                 HBII-180A_x 
                 −1.43 
                 4.25614E−12 
                 0.992 
                 CDBox 
                 123 
               
               
                 14qll-12_x 
                 −2.09 
                 4.30741E−12 
                 0.949 
                 CDBox 
                 85 
               
               
                 miR-509-3-5p 
                 3.46 
                 5.53398E−12 
                 0.953 
                 miRNA 
                 107 
               
               
                 U55_x 
                 −1.35 
                  6.7836E−12 
                 0.996 
                 CDBox 
                 135 
               
               
                 miR-149-star 
                 0.93 
                  9.6022E−12 
                 0.940 
                 miRNA 
                 135 
               
               
                 HBII-276 
                 −1.41 
                 1.19464E−11 
                 0.964 
                 CDBox 
                 132 
               
               
                 miR-23b 
                 −0.75 
                 1.72105E−11 
                 0.968 
                 miRNA 
                 137 
               
               
                 miR-15b 
                 −2.42 
                  1.7823E−11 
                 0.968 
                 miRNA 
                 125 
               
               
                 miR-921 
                 1.73 
                 1.93285E−11 
                 0.954 
                 miRNA 
                 49 
               
               
                   
               
             
          
         
       
     
     Example 4 
     The data from Example 3 was further analyzed to identify the combination of markers best able to distinguish (A) melanoma from normal skin and (B) melanoma from nevi. Each miRNA (“analyte”) was examined by svm, random forest, boosting, lasso, baggin, cart, matt, logistic regression, and ctree analyses. The use of multiple statistical method demonstrates that the best combination of markers identified by one statistical method is validated by every other method, making the choice of marker combinations less subject to the specific weaknesses of any one statistical algorithm. 
       FIGS. 1-10  show, respectively, the best 2-10 miRNA combinations for differentiating melanoma (MM) from normal skin (“NS”) and the relevant error rates, as determined by different statistical algorithms. 
       FIGS. 11-23  show, respectively, the best 2-14 miRNA combinations for differentiating melanoma (MM) from nevi (NV), and the relevant error rates, as determined by different statistical algorithms. 
       FIG. 24  shows the log 2 signal for three miRNA: miR-150 (R-150), miR-149-star (miR-149-star), and hsa-miR-1308 (miR-1308) across 137 samples. Samples 1-19 are normal skin (NS), 20-57 nevi (NV), 58-115 melanoma (MM) and 116-137 metastatic melanoma (Mets). As can be appreciated, miR-150, miR-149-star and miR-1308 distinguish normal skin and nevi from melanoma and metastatic melanoma. 
     Example 5 
     In a follow up study, a total of 78 melanoma and 98 nevi (Intradermal-20, Compound-18, Junctional-20, Blue-20, Spitz-20) were studied on microarray. The candidate microRNAs were chosen based on AUC, p value, log 2 fold change, and 9 analysis programs, as before. A example of error rate and AUC from 9 programs is set forth in  FIG. 25 . The candidate microRNAs identified from 2 programs (random forest and boosting) are listed in Table 9, below. 
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 MicroRNA candidates from 78 melanoma and 98 nevi 
               
             
          
           
               
                 microrna 
                 adj.P.Val (FDR) 
                 AUC 
                 Log2FC 
                 Order in program 
               
               
                   
               
             
          
           
               
                 miR-1268 
                 1.45E−19 
                 0.89 
                 −1.80 
                 5 
               
               
                 miR-1228-star 
                 2.87E−19 
                 0.91 
                 −1.77 
                 2 
               
               
                 miR-92b-star 
                 4.62E−18 
                 0.88 
                 −1.73 
                 3 
               
               
                 miR-155 
                 5.56E−18 
                 0.86 
                 2.85 
                 4 
               
               
                 miR-345 
                 1.19E−16 
                 0.85 
                 1.83 
                 7 
               
               
                 miR-425 
                 5.11E−16 
                 0.85 
                 1.80 
               
               
                 miR-132 
                 5.24E−16 
                 0.85 
                 1.75 
                 10 
               
               
                 miR-1207-5p 
                 1.18E−15 
                 0.86 
                 −1.40 
               
               
                 miR-1301 
                 2.17E−15 
                 0.83 
                 1.70 
               
               
                 miR-663 
                 2.26E−15 
                 0.85 
                 −1.43 
               
               
                 miR-339-5p 
                 2.26E−15 
                 0.85 
                 1.60 
                 9 
               
               
                 miR-149-star 
                 5.28E−15 
                 0.85 
                 −1.32 
               
               
                 miR-150 
                 5.25E−14 
                 0.82 
                 2.10 
                 6 
               
               
                 miR-18a 
                 5.80E−13 
                 0.8 
                 0.84 
               
               
                 miR-103 
                 1.52E−11 
                 0.86 
                 0.93 
                 8 
               
               
                 miR-191 
                 7.32E−13 
                 0.9 
                 0.85 
                 1 
               
               
                 miR-296-3p 
                 1.05E−09 
                 0.79 
                 −0.85 
                 11 
               
               
                 miR-31 
                 1.39E−12 
                 0.79 
                 2.46 
               
               
                 miR-107* 
                 3.27E−10 
                 0.85 
                 0.86 
               
               
                 miR-93* 
                 1.05E−09 
                 0.85 
                 1.14 
               
               
                 miR-1275* 
                 4.59E−11 
                 0.8 
                 −1.16 
               
               
                 miR-181B* 
                 3.69E−12 
                 0.83 
                 1.69 
               
               
                 miR-921* 
                 7.21E−12 
                 0.81 
                 −0.89 
               
               
                 miR-1225-5p 
                 7.63E−10 
                 0.78 
                 −1.3 
               
               
                 miR-1202 
                 1.08E−09 
                 0.76 
                 −1.34 
               
               
                 miR-342-3p 
                 1.55E−10 
                 0.78 
                 1.29 
               
               
                 Internal control 
               
               
                 candidates 
               
               
                 miR-27b 
                 0.72 
                 0.07 
                 0.501 
               
               
                 miR-195 
                 0.95 
                 0.01 
                 0.506 
               
               
                 miR-199b-3p 
                 0.78 
                 0.05 
                 0.509 
               
               
                 miR-199a-3p 
                 0.86 
                 0.03 
                 0.511 
               
               
                   
               
             
          
         
       
     
     REFERENCES 
     
         
         1. Leidinger P, Keller A, Borries A, Reichrath J, Rass K, Jager S U, Lenhof H P, Meese E. High-throughput miRNA profiling of human melanoma blood samples. BMC Cancer. 2010 Jun. 7; 10:262. 
         2. Demetra Philippidou, Martina Schmitt, Dirk Moser, Christiane Margue, Petr V Nazarov, Arnaud Muller, Laurent Vallar, Dorothee Nashan, Iris Behrmann and Stephanie Kreis Signatures of MicroRNAs and Selected MicroRNA Target Genes in Human Melanoma. Cancer Res 2010 May 70(10); 4163-4173 
         3. Segura F, Ilana Belitskaya-Lévy I, Rose A, Zakrzewski J, Gaziel A. Melanoma MicroRNA Signature Predicts Post-Recurrence Survival Clinical Cancer Research 2020 March 16(5); 1577-1586 
       
    
     The contents of the articles, patents, and patent applications, and all other documents and electronically available information mentioned or cited herein, are hereby incorporated by reference in their entirety to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference. Applicants reserve the right to physically incorporate into this application any and all materials and information from any such articles, patents, patent applications, or other physical and electronic documents. 
     The inventions illustratively described herein may suitably be practiced in the absence of any element or elements, limitation or limitations, not specifically disclosed herein. Thus, for example, the terms “comprising”, “including,” containing”, etc. shall be read expansively and without limitation. Additionally, the terms and expressions employed herein have been used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the inventions embodied therein herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention. 
     The invention has been described broadly and generically herein. Each of the narrower species and subgeneric groupings falling within the generic disclosure also form part of the invention. This includes the generic description of the invention with a proviso or negative limitation removing any subject matter from the genus, regardless of whether or not the excised material is specifically recited herein. 
     Other embodiments are within the following claims. In addition, where features or aspects of the invention are described in terms of Markush groups, those skilled in the art will recognize that the invention is also thereby described in terms of any individual member or subgroup of members of the Markush group.