Abstract:
The present invention discloses and claims benzimidazole compounds of formula (I):                          
 
in which A is aryl or heteroaryl; R 1  is selected from optionally substituted alkyl, alkoxy, aryl or heteroaryl, NH-lower alkyl or NH-cycloalkyl, or halogen, NH 2 ; 1-imidazolyl or SO 2 Me; R 2  is selected from optionally substituted —CO-alkyl, —CO-cycloalkyl, —CO-aralkyl, —CO-aryl, —CO-alkoxy, aryl or aralkyl, or —CO-amino, CO—NHR 3  or CO—R 3 R 4  wherein R 3  and R 4  are selecteded independently from hydrogen, alkyl, hydroxyalkyl, alkoxyalkyl, fluoroalkyl, alkynyl, heteroalkyl, alkylheteroalkyl, aryl, aralkyl or together form an alkylene chain optionally containing one to 4 heteroatoms; a pharmaceutically acceptable salt or a prodrug thereof; the use of compounds of formula (I) for the treatment of cancer, and pharmaceutical compositions comprising a compound of formula (I) and one or more pharmaceutically acceptable adjuvants or diluents.

Description:
This application is a continuation of International Application No. PCT/EP02/11353 filed Sep. 26, 2002, which claims the benefit of priority of European Application No. 01 402 460.8, filed Sep. 26, 2001. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to compounds useful for treating pathological states, which arise from or are exacerbated by cell proliferation, to pharmaceutical compositions comprising these compounds, and to methods of inhibiting cell proliferation in a mammal. 
     2. Description of the Art 
     Neoplastic diseases, characterized by the proliferation of cells, which are not subject to normal cell proliferating controls, are a major cause of death in humans and other mammals. Cancer chemotherapy has provided new and more effective drugs to treat these diseases and has also demonstrated that drugs, which are inhibitors of cyclin-dependent kinases are effective in inhibiting the proliferation of neoplastic cells. 
     Regulators at cell cycle checkpoints determine the decision for a cell to proceed through the cell cycle. Progression of the cell cycle is driven by cyclin-dependent kinases (CDKs) which are activated by oscillating members of the cycin family, resulting in substrate phosphorylation and ultimately cell division. In addition, endogenous inhibitors of CDKs (INK4 family and KIP/CIP family) negatively regulate the activity of CDKs. Normal cell growth is due to a balance between activators of CDKs (cyclins) and endogenous inhibitors of CDKs. In several types of cancer, aberrant expression or activity of several components of the cell cycle has been described. 
     Cdk4 functions in G1 phase of the cell cycle and is activated by D-type cyclins, which results in substrate phosphorylation and progression to S phase. The only known substrate for cdk4 is the retinoblastonia gene product (pRb), a major tumor suppressor gene product, which functions as a major checkpoint control in regulation of the G1/S phase transition. Hyperphosphorylation of pRb by CDKs causes the release of E2F (a family of transcription factors) bound to pRb which then activate genes necessary for cell cycle progression, e.g. thymidine kinase, thymdylate synthase, cyclin E and cyclin A. Cycin DI is amplified or overexpressed in many types of cancer (breast, ovarian, bladder, esophageal, lung, lymphoma), while the gene for p16, the endogenous inhibitor of cdk4, is deleted, mutated, or aberrantly methylated in many tumor types. A point of mutation in cdk4 was reported in a melanoma tumor that rendered the enzyme unable to bind p16 resulting in a constitutively active enzyme. All of the conditions described above lead to activation of cdk4 and cell cycle progression and tumor cell growth. 
     Arguments to designate CDK2 as an anticancer agent can be found in the literature &lt;&lt;Cyclin E activates Cdk2 which acts to phosphorylate pRb resulting in an irreversible commitment to cell division and transition into S-phase&gt;&gt; (P. L. Toogood, Medicinal Research Reviews (2001), 21(6); 487–498. and &lt;&lt;CDK2 (and possibly CDK3) is required for G1 progression and entry into S phase. In complex with cyclin E, it sustains pRb hyperphosphrylation to support progression through G1 and into S phase. In addition many other cellular targets of CDK2-CyclinE have been identified . . . . In complex with cyclinA, CDK2 plays a role in inactivating E2F and is required for completion of S phase.&gt;&gt; T. D. Davies et al. (2001) Structure 9, 389–397. 
     An added level of regulation of CDK activity exists. Cyclin-dependent kinase activating kinase (CAK) is a positive regulator of CDKs. CAK phosphorylates the catalytic CDKs on a conserved threonine residue to render the target enzyme completely active. 
     Because the defects in cell cycle molecules lead to CDK activation and subsequently cell cycle progression, it is logical that inhibition of CDK enzyme activity should block cell cycle progression and tumor cell growth. 
     The first CDK inhibitor to enter clinical trials is the compound known as flavopiridol. This compound is currently in Phase II clinical trials and is the only molecule in its class in the clinic at the present time. The aim of this invention is to produce molecules more active than flavopiridol. 
     It is known following publication of WO00/41669 that benzimidazole carbamate derivatives are vascular damaging agents that can be used for treating cancer, the sulfonic acid ester derivatives claimed in this patent application are not at all exemplified and their anticancerous way of action is not described. Our invention relates specifically to carbamates of those sulfonic acid ester derivatives. 
     SUMMARY OF THE INVENTION 
     In one embodiment of the present invention are disclosed compounds of formula (I) 
     
       
                 
         
             
             
         
      
         
         
           
             wherein A is an aryl or heteroaryl entity 
             wherein R 1  is selected from the group consisting of
           alkyl, eventually substituted by an alkoxy, heteroalkyl, aryl, acyl, acyl derivatives, halogen   alkoxy eventually substituted by an alkyl, heteroalkyl, aryl, heteroaryl, alkoxyalkyl, hydroxyalkyl amide or a perfluoroalkoxy group or an alkylthio eventually substituted by an amide or a perfluoroalkylthio   aryl or heteroaryl eventually substituted by one or more alkyl group, alkoxy group, nitro group, cyano group, acyl derivative, perfluoroalkoxy group, perfluoroalkyl group, heteroaryl group, aryloxy group   halogen   4 NH 2      4 NH alkyl or cycloalkyl eventually substituted with an an acyl, an acyl derivative, an hydroxy, an amino, alkoxy, heterocyclyl or aryl group   4 N imidazolyl   3 SO 2 Me when A is phenyl   
         
             wherein R2 is selected from the group consisting of
           CO-alkyl eventually substituted by amino, acid, acid derivative, alkoxy, aryl or OH groups   CO-aralkyl eventually substituted by alkoxy, halogeno, amino, acid or acid derivatives   CO-aryl eventually substituted   CO-alkoxy eventually substituted by aryl   CO-amino, CO—NHR 3 , CO—NR 3 R 4  wherein R 3  and R 4  are selected independently from hydrogen, alkyl, hydroxyalkyl, alkoxyalkyl, fluoroalkyl, alkynyl, heteroalkyl, alkylheteroalkyl, aryl, aralkyl or together form an alkylen chain including eventually one to 4 more heteroatoms   aryl or aralkyl eventually substituted by heterocycloalkyl, alkyl, aryl, alkoxy, amino, fluoroalkyl, acyl derivatives, halogen
 
or a pharmaceutically acceptable salt.
   
         
           
         
       
    
     Among the preferred compounds of formula (I) are those where A represents a phenyl, thiophene, isoxazole, oxazole, pyrazole, furan, or pyridine, and more preferably those where A is a phenyl group. 
     Among the preferred compounds of formula (I) are those wherein the aryl, aralkyl, heteroaryl or heteroarylalkyl are optionally substituted with one or more similar or different groups selected from halogen, alkoxy, alkyl, hydroxyalkyl, alkylthio, amino, mono or dialkylamino, heterocyclylamino, arylamino, heteroarylamino, heteroaryl, nitro, heterocycloalkyl, perfluoroalkyl, perfluroroalkoxy, perfluoroalkylthio, acyl derivatives. 
     Among the preferred compounds of formula (I) are those wherein R 2  is an aminocarbonyl group substituted by a substituent selected from monoalkylamino or a monoarylamino sutstituent. In the preferred compounds of formula (I) are those containing for R 2  an amino substituent and preferably a monoalkylamino or a monoarylamino substituent and still more preferably those containing a monoalkylamino substituent with an acyl derivative. 
     Among the alkyl or alkylene substituents which are substituted are included those substituted with one or more amino, aminoalkyl, aminoalkylamino, hydroxy, alkoxy, hydroxyalkoxy, acyl, acyl derivatives, alkyl, heteroalkyl, arylalkyl, arylamino, aryloxy, or aryl groups. 
     Among the alkoxy or alkythio substituents are included the alkoxy or alkylthio groups substituted with one or more amino, acyl, acyl derivatives, alkyl, arylalkyl or aryl groups. 
     Among the acyl groups or acyl derivatives groups are included the carboxylic acids and the sulfonic acids, the derivatives of which being mainly ester or carbamoyl esters. 
     The alkyl chain of the present invention includes linear, branched or cyclic chain containing 1 to 10 carbon atoms. The alkoxy chain of the present invention includes linear, branched or cyclic chains containing 1 to 4 carbon atoms. The aryl groups include phenyl or naphthyl groups, heteroaryl groups containing one to four heteroatoms selected from S, N or O such as furanyl, thiophenyl, isoxazolyl, oxazolyl, pyrazolyl and pyridinyl. The heterocyclyl group contains one to four heteroatoms chosen from N, O, S and 2 to 6 carbon atoms. 
     Among the preferred compounds are those containing an alkyl chain 1 to 10 carbon atoms and those containing a cycloalkyl chain 3 to 5 carbon atoms. When the alkyl chain is substituted by an alkoxy group this last group has preferably one carbon atom. 
     Among the compounds of formula (I) the following compounds are much more preferred:
     Methyl-5-(4-[2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[4-hydroxbutyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[2-methoxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[1-imidazolyl]-phenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[2-pyridylmethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-ethylaminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[N-glycinyl]-phenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[1-methyl,2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[2-methyl,2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-isopropylaminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[1-ethyl 2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-butylaminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[3-methoxypropyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-methylaminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[2-sulfonylethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[2-diethylaminoethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[1-tetrathydrofurylmethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate   Methyl-5-(4-[2-phenylethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate   N-[5-(4-[imidazolyl]-phenylsulfonyloxy)-1H-benzimidazole-2-yl]-methylurea   N-[5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-yl]-methylurea   N-[5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-yl]-dimethylurea   4-Imidazol-1-yl-benzenesulfonic acid 2-benzoylamino-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-phenylacetylamino-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(2-tert-butoxycarbonylamino-acetylamino)-1H-benzoimidazol-5-yl ester   N-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-succinamic acid methyl ester   4-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-butyric acid methyl ester   4-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-butyric acid methyl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(cyclohexanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[(pyridine-2-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[(pyridine-3-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[(pyridine-4-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-pentanoylamino-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-hexanoylamino-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(2-cyclopropyl-acetylamino)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(2-cyclohexyl-acetylamino)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(2-methoxy-acetylamino)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(2-dimethylamino-acetylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-benzoylamino-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-phenylacetylamino-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(2-tert-butoxycarbonylamino-acetylamino)-1H-benzoimidazol-5-yl ester   N-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-succinamic acid methyl ester   4-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-butyric acid methyl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(cyclohexanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[(pyridine-2-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[(pyridine-4-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[(pyridine-4-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-pentanoylamino-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-hexanoylamino-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(2-cyclopropyl-acetylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(2-cyclohexyl-acetylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(2-methoxy-acetylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(2-dimethylamino-acetylamino)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-cyclopropyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-cyclopropyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-isopropyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-isopropyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-butyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-butyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(3-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(4-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(4-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-methoxy-benzyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-methoxy-benzyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-fluoro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(3-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-isobutyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-isobutyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-dimethylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-dimethylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-ethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-ethyl-ureido)-1H-benzoimidazol-5-yl ester   {3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-acetic acid   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-sulfo-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(4-dimethylamino-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-dimethylamino-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-cyclobutyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-cyclobutyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-pyridin-4-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-pyridin-4-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-tert-butyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-tert-butyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-phenyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-phenyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-cyclohexyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-cyclohexyl-ureido)-1H-benzoimidazol-5-yl ester;   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-cyclopentyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(3-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-hydroxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-hydroxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(4-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-chloro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-chloro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-fluoro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-fluoro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[(azetidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[(azetidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-pyridin-3-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-pyridin-3-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-{3-[3-(4-methyl-piperazin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-{3-[3-(4-methyl-piperazin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-benzyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-benzyl-ureido)-1H-benzoimidazol-5-yl ester   4-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-butyric acid methyl ester;   4-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-butyric acid ethyl ester   4-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-acetic acid methyl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-imidazol-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   1-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2S-ylcarbamoyl]-pyrrolidine-2-carboxylic acid   1-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2S-ylcarbamoyl]-pyrrolidine-2-carboxylic acid methyl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-carbamoylmethyl-ureido)-1H-benzoimidazol-5-yl ester   1-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-piperidine-4-carboxylic acid ethyl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-piperidin-4-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-amino-2-methyl-propyl)-ureido]-1H-benzoimidazol-5-yl ester;   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-hydroxy-cyclohexyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(1,1-dimethyl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-{3-[2-(2-hydroxy-ethylamino)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-hydroxy-butyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-{3-[3-(2-oxo-pyrrolidin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-carbamoyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[(2S-carbamoyl-pyrrolidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-{3-[2-(2-hydroxy-ethoxy)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-pyrrolidin-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(1-ethyl-pyrrolidin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-pyrrolidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-hydroxy-1-methyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-isopropylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-diethylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   2-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-3S-hydroxy-propionic acid methyl ester   4-{3-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-butyric acid methyl ester   4-{3-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-butyric acid ethyl ester   {3-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-acetic acid methyl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(3-imidazol-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   1-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-pyrrolidine-2-carboxylic acid   1-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-pyrrolidine-2-carboxylic acid methyl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-carbamoylmethyl-ureido)-1H-benzoimidazol-5-yl ester   1-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-piperidine-4-carboxylic acid ethyl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-(3-piperidin-4-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-amino-2-methyl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(4-hydroxy-cyclohexyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(1,1-dimethyl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-{3-[2-(2-hydroxy-ethylamino)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(4-hydroxy-butyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-{3-[3-(2-oxo-pyrrolidin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-carbamoyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(1,1-dimethyl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[(2-carbamoyl-pyrrolidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-{3-[2-(2-hydroxy-ethoxy)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(3-pyrrolidin-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(1-ethyl-pyrrolidin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-pyrrolidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-hydroxy-1-methyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-isopropylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-diethylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   2-{3-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-3-hydroxy-propionic acid methyl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-carbamoylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-hydroxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(4-hydroxy-butyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-methoxy-1-methyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(1-ethyl-pyrrolidin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-pyrrolidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(3-pyrrolidin-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-ethyl-ureido)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-methyl-ureido)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-3-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-{3-[3-(4-methyl-piperazin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-sulfo-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-cyclobutyl-ureido)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-{3-[3-(2-oxo-pyrrolidin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-{3-[2-(2-hydroxy-ethylamino)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Methylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Hydroxy-butylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Methoxy-1-methyl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Pyrrolidin-1-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1-Hydroxymethyl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Piperidin-1-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Pyrrolidin-1-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Hydroxy-2,2-dimethyl-propylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Pyridin-3-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[3-(4-Methyl-piperazin-1-yl)-propylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Methoxy-benzylamino)benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Hydroxy-cyclohexylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Diethylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1S-Hydroxymethyl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Ethylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Diisopropylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1-Aza-bicyclo[2.2.2]oct-3-ylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Phenylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1-Benzyl-pyrrolidin-3-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2R-Carbamoyl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Dimethylamino-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-piperazin-1-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Carbamoyl-cyclohexylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Acetylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(2-Amino-ethylamino)-ethylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[3-(2-Oxo-pyrrolidin-1-yl)-propylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(1H-Imidazol-4-yl)-ethylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Cyclobutylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(2-Hydroxy-ethoxy)-ethylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2,3-Dihydroxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Imidazol-1-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(2-Hydroxy-ethylamino)-ethylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Dimethylamino-1-methyl-ethylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(Pyrrolidin-3-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(1H-Indol-3-yl)-ethylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Dimethylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Phenoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(Bicyclo[2.2.1]hept-2-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Methylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Propylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1-Methyl-2-phenoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Piperidin-4-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Methoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1H-Benzoimidazol-5-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Methoxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2,2-Dimethoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Dimethylamino-phenylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Methoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Pyrrolidin-1-yl-butylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2,3-Dimethoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Prop-2-ynylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[4-(2-Hydroxy-ethyl)-piperazin-1-yl]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Pyridin-4-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(Ethyl-m-tolyl-amino)-ethylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-cyclohexylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Dimethylamino-2,2-dimethyl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[3-(2-Hydroxy-ethylamino)-propylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2R-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Tetrahydro-furan-2S-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Butylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Methylamino-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1S,2-Dicarbamoyl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   2-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-3R-hydroxy-propionic acid methyl ester   4-(2-Carbamoyl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Methoxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3,4,5-Trimethoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(Carbamoylmethyl-amino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   1-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperidine-4-carboxylic acid ethyl ester   4-(2-Amino-2-methyl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   3-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-propionic acid methyl ester   4-(3-Morpholin-4-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(5-Hydroxy-pentylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(5S-Amino-2,2,4S-trimethyl-cyclopentylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Hydroxymethyl-phenylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Ethoxy-phenylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Ethylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Sulfo-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-piperidine-1-carboxylic acid ethyl ester   4-({4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-methyl)-benzoic acid   4-[(1-Carbamimidoyl-piperidin-4-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperazine-1-carboxylic acid tert-butyl ester   3-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-3-phenyl-propionic acid   4-Piperidin-1-yl-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1-Methyl-4-oxo-imidazolidin-2-ylideneamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Methyl-piperazin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Hydroxy-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(2-Dimethylamino-ethyl)-methyl-amino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Isobutylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[Ethyl-(2-hydroxy-ethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-1-hydroxymethyl-ethylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Propylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Cyclopropylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Morpholin-4-yl-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(1-Methyl-pyrrolidin-2-yl)-ethylamino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(1,3-Dimethyl-1H-pyrazol-4-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Acetylamino-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Cyclohexylamino-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Ethoxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-Pyrrolidin-1-yl-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Methyl-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[1,4′]Bipiperidinyl-1′-yl-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Pyridin-3-yl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(2-Hydroxy-ethyl)-methyl-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(1-Ethyl-pyrrolidin-2-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Hydroxy-pyridin-2-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(1-Carbamoyl-piperidin-4-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Pyrrol-1-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Cyclopentyl-piperazin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Propoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Cyclohexylamino-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1H-Indol-5-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Amino-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2S-Methoxymethyl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[4-(2-Hydroxy-ethyl)-piperidin-1-yl]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(2-Hydroxy-ethyl)-piperidin-1-yl]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Isopropylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   3-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-propionic acid   4-[Methyl-(2-methylamino-ethyl)-amino]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Acetylamino-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(Carbamoylmethyl-amino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Dimethylamino-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Imidazol-1-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(Quinoxalin-5-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[4-(2-Hydroxy-ethyl)-piperazin-1-yl]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-1,1-dimethyl-ethylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   1-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperidine-4-carboxylic acid   6-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-hexanoic acid methyl ester   4-[4-(4-Methoxy-phenyl)-piperazin-1-yl]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[4-(2-Methoxy-ethyl)-piperazin-1-yl]-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(2-Hydroxy-ethyl)-phenyl-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[(Furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   1-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-aziridine-2-carboxylic acid methyl ester   4-(4-Carbamoyl-piperidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Methyl-piperazin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2,6-Dimethyl-morpholin-4-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Phenyl-piperazin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Pyridin-2-yl-piperazin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Diethylamino-1-methyl-butylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperazine-1-carboxylic acid ethyl ester   4-(5-Hydroxy-naphthalen-1-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(4-Hydroxy-3-methoxy-phenyl)-ethylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(9H-Purin-6-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   1-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperidine-3-carboxylic acid   4-(3,3-Dimethyl-piperidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Methyl-piperidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Pyridin-2-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Hydroxymethyl-phenylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2-Oxo-2,3-dihydro-1H-pyrimidin-4-ylideneamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Piperidin-1-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[2-(1H-Indol-3-yl)-ethylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(5-Carbamoyl-1H-imidazol-4-ylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1-Hydroxymethyl-butylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(1-Benzyl-piperidin-4-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-{4-[2-(2-Hydroxy-ethoxy)-ethyl]-piperazin-1-yl}-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(4-Methyl-[1,4]diazepan-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(3-Azepan-1-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2,6-cis-Dimethyl-morpholin-4-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-(2S-Hydroxymethyl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester   4-[4-(3-Pyrrolidin-1-yl-propyl)-[1,4]diazepan-1-yl]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester   4-trifluoromethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   3,5-Dimethyl-isoxazole-4-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   Thiophene-2-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   5-Isoxazol-3-yl-thiophene-2-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   2-Fluoro-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   5-(1-Methyl-5-trifluoromethyl-1H-pyrazol-3-yl)-thiophene-2-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   3-Trifluoromethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   2-Trifluoromethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzo-imidazol-5-yl ester   2,6-Difluoro-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   3-Methoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   3-(2-Methoxycarbonylamino-1H-benzoimidazol-5-yloxysulfonyl)-thiophene-2-carboxylic acid methyl ester   3,4-Dimethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   3-Nitro-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   3-Trifluoromethyl-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzo-imidazol-5-yl ester   2-Cyano-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   2-Trifluoromethyl-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzo-imidazol-5-yl ester   2,4-Difluoro-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   5-Fluoro-2-methyl-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzo-imidazol-5-yl ester   3-Fluoro-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-Cyano-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   2-Methoxy-5-(2-methoxycarbonylamino-3H-benzoimidazol-5-yloxysulfonyl)-thiophene-3-carboxylic acid methyl ester   1,3,5-Trimethyl-1H-pyrazole-4-sulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester   6-Morpholin-4-yl-pyridine-3-sulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester   2,4,6-Trifluoro-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-benzyloxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-Ethoxy-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester   4-(2-Morpholin-4-yl-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester   4-(2-Methoxy-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester   4-(2-piperidin-1-yl-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester   [4-(2-Methoxycarbonylamino-3H-benzoimidazol-5-yloxysulfonyl)-phenoxy]-acetic acid   4-(2-oxo-2-pyrrolidin-1-yl-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl-ester   4-[2-(4-Methyl-piperazin-1-yl)-2-oxo-ethoxy]-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-[(3-diethylamino-propylcarbamoyl)-methoxy]-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-{[(furan-2-ylmethyl)-carbamoyl]-methoxy}-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-(cyclopropylmethyl-amino)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-(2-methoxy-ethylamino)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-(2-hydroxy-1-methyl-ethylamino)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-(benzylamino)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester   4-(2-Piperidin-4-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-[(1-Ethyl-pyrrolidin-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-cyclopentylamino-benzenesulfonic acid 2-(3,4-dimethoxy-phenylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-phenylamino-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(4-morpholin-4-yl-phenylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3,5-dimethyl-phenylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(4-methoxy-phenylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(4-dimethylamino-phenylamino)-1H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-methoxy-5-trifluoromethyl-phenylamino)-1H-benzoimidazol-5-yl ester   3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-ylamino]-benzoic acid ethyl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[(4-(4-methyl-piperazin-1-yl)-phenylamino)-1H-benzoimidazol-5-yl ester   4-cyclopentylamino-benzenesulfonic acid 2-(3-phenyl-propionylamino)-1H-benzoimidazol-5-yl ester   4-cyclopentylamino-benzenesulfonic acid 2-[2-2-methoxy-ethoxy)-acetylamino]-1H-benzoimidazol-5-yl ester   4-fluoro-benzenesulfonic acid 2-(3(chloro-4-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester   4-Fluoro-benzenesulfonic acid 2-[(3-phenyl-[1,2,4]oxadiazol-5-ylmethyl)-amino]-3H-benzoimidazol-5-yl ester   4-Fluoro-benzenesulfonic acid 2-(3-chloro-benzylamino)-3H-benzoimidazol-5-yl ester   4-Fluoro-benzenesulfonic acid 2-(3-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester   4-Fluoro-benzenesulfonic acid 2-benzylamino-3H-benzoimidazol-5-yl ester   4-cyclopentylamino-benzenesulfonic acid 2-benzylamino-3H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-[(3-phenyl-[1,2,4]oxadiazol-5-ylmethyl)-amino]-3H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester   4-Cyclopentylamino-benzenesulfonic acid 2-(3-chloro-4-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester   4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzyloxy-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzyloxy-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzyloxy-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-benzyloxy-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Piperidin-4-ylmethyl)-amino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Piperidin-4-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(Piperidin-4-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzylamino-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzylamino-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzylamino-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-Benzylamino-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-[(1-ethyl-pyrrolidin-2ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[(4-hydroxy-piperidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester   4-(4-Methyl-piperazin-1-yl)-benzenesulfonic acid 2-[(4-methyl-piperazin-1-carbonyl)-amino]-3H-benzoimidazol-5-yl ester   4-[(tetrahydro-pyran-4-ylmethyl)-amino]-benezenesulfonic acid 2-[3-(tetrahydro-pyran-4-ylmethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(2-Fluoro-ethylamino)-benzenesulfonic acid 2-[3-(2-fluoro-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(2-piperidin-1-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-phenethylamino-benzenesulfonic acid 2-(3-phenethyl-ureido)-3H-benzoimidazol-5-yl ester   4-[3-(2-oxo-pyrrolidin-1-yl)-propylamino]-benzenesulfonic acid 2-{3-[3-(2-oxo-pyrrolidin-1-yl)-propyl]-ureido}-3H-benzoimidazol-5-yl ester   4-(4-fluoro-benzylamino)-benzenesulfonic acid 2-[3-(4-fluoro-benzyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(2-hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-[3-(2-hydroxy-3-methyl-propyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(3-hydroxy-propylamino)-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(2,2,6,6-tetramethyl-piperidin-4-ylamino)-benzenesulfonic acid 2-[3-(2,2,6,6-tetramethyl-piperidin-4-yl)-ureido]-3H-benzoimidazol-5-yl ester   4-(2-dimethylamino-ethylamino)-benzenesulfonic acid 2-[3-(2-dimethylamino-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-morpholin-4-yl-benzenesulfonic acid 2-[(morpholine-4-carbonyl)-amino]-3H-benzoimidazol-5-yl ester   4-(2-Hydroxy-3-methoxy-propylamino)-benzenesulfonic acid 2-[3-(2-hydroxy-3-methoxy-propyl)-ureido]-3H-benzoimidazol-5-yl ester   4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-yl-ethyl-ureido)-3H-benzoimidazol-5-yl ester   4-(2-hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-hydroxy-propyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(4-methoxy-benzyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-pyrrolidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(1-phenyl-ethylamino)-benzenesulfonic acid 2-[3-(1-phenyl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(2-diethylamino-ethylamino)-benzenesulfonic acid 2-[3-(2-diethylamino-ethyl)-ureido]-3H-benzoimidazol-5-yl ester   4-(1-hydroxymethyl-cyclopentylamino)-benzenesulfonic acid 2-[3-(1-hydroxy-methyl-cyclopentyl)-ureido]-3H-benzoimidazol-5-yl ester   3-(4-{2-[3-(3-Methoxycarbonyl-ethyl)-ureido]-1H-benzoimidazol-5-yloxysulfonyl}-phenylamino)-propionic acid methyl ester   4-(4-Hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-(4-methyl-piperazin-1-yl)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(4-methyl-piperazin-1-yl)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(4-methyl-piperazin-1-yl)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2,2,6,6-tetramethyl-piperidin-4-ylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2,2,6,6-tetramethyl-piperidin-4-ylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2-dimethylamino-ethylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-dimethylamino-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-morpholin-4-yl-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-morpholin-4-yl-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-[3-(2-oxo-pyrrolidin-1-yl)-propylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-[3-(2-oxo-pyrrolidin-1-yl)-propylamino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid-2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester   4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-fluoro-ethylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-fluoro-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2-Piperidin-1-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-Piperidin-1-yl-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-phenethylamino-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-phenethylamino-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-phenethylamino-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-phenethylamino-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-hydroxy-propylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester   4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester   4-(1-phenyl-ethylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester   4-(2-diethylamino-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester   Thiophene-2-sulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   Thiophene-2-sulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   Thiophene-2-sulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester   Thiophene-2-sulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester   4-{2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yloxysulfonyl}-phenyl ester   Benzoic acid 4-{2-[3-(2-morpholin-4-yl-ethyl)-ureido}-1H-benzoimidazol-5-yloxy-sulfonyl}-phenyl ester   Benzoic acid 4-[2-([3-pyridin-2-ylmethyl)-ureido)-1H-benzoimidazol-5-yloxy-sulfonyl]-phenyl ester   2,6-difluoro-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzoimidazol-5-yl ester   2,6-difluoro-benzenesulfonic acid 3-(2-methoxy-ethyl)-3H-benzoimidazol-5-yl ester   

    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     In still yet another embodiment is disclosed use of compounds of formula (I) for treating cancer diseases. 
     In still yet another embodiment is disclosed a method of inhibiting CDK4 enzymes in a mammal in recognized need of such treatment comprising administering to the mammal a therapeutically effective amount of compounds of formula (I). 
     In still yet another embodiment is disclosed a pharmaceutical composition which comprises a therapeutically effective amount of a compound of formula (I) in combination with a pharmaceutically acceptable carrier. 
     The term “pharmaceutically acceptable salt”, as used herein, refers to salts, which are suitable for use in contact with the tissues of humans and lower animals. Pharmaceutically acceptable salts are described in detail in J. Pharmaceutical Sciences, 1977, 66:1 et seq. hereby incorporated by reference. Representative acid addition salts include acetate, citrate, aspartate, benzenesulfonate, hydrochloride, lactate, maleate, methanesulfonate, oxalate, and phosphate. 
     Chemical Synthesis 
     Compounds of the present invention can be easily prepared starting from 2-amino-5-(-4-fluorophenylsulfonyloxy)nitrobenzene, the process of preparation of which is described in U.S. Pat. No. 3,996,368. 
     In a first step this starting material is reacted with the amine bearing the R1 radical in a suitable solvent for carrying out the reaction. Among the list of solvents suitable for dissolving 2-amino-5-(-4-fluorophenylsulfonyloxy)nitrobenzene and the amine can be cited the glycols such as ethyl glycol, and the aprotic solvents such as dioxane, dimethylformamide, N-methylpyrrolidone. The preferred temperature for this reaction is comprised between room temperature and the reflux temperature. To recover the intermediate product it is preferred to precipitate the intermediate with hydrochloric acid. 
     In a second step the compound of step 1 is hydrogenated with hydrogen preferably in presence of Raney nickel (nitro group reduction method A) or palladium on carbon (nitro group reduction method B) in a suitable solvent choosen among the same list as for step 1 in mixture with an alcohol such as methanol. After reaction the catalyst is taken off by filtration. 
     In a third step the benzimidazole ring is closed by action of 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea on the intermediate obtained in step 2 without intermediate separation. The reaction mixture is heated to reflux with stirring. The final product (methyl-benzimidazole-2-carbamate) is isolated after evaporation of the solvent under reduced pressure and solubilization in ethyl acetate then crystallisation. A final purification is carried out in methanol with a crystallisation in the same solvent. 
     Methyl-benzimidazole-2-carbamate can be converted to benzimidazole-2-ureas by treatment with an amine in a suitable solvent such as dimethylformamide, tetrahydrofuran or N-methylpyrrolidone in the presence of a base such as 1,8-diazabicyclo[5.4.0]undec-7-ene in a pressure vessel. The preferred temperature for this reaction is comprised between room temperature and 120° C. 
     tert-Butyl-benzimidazole-2-carbamate can be prepared by performing the third step described above using 1,3-bis(tert-butoxycarbonyl)-2-methyl-2-thiopseudourea instead of 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea. These derivatives can be converted to the corresponding 2-aminobenzimidazole derivative using tert-butylcarbamate deprotection methods known by the persons skilled in the art. The 2-aminobenzimidazoles can be converted to the corresponding amides by reaction with carboxylic acid derivatives using methods known by the persons skilled in the art. 
     Formulations 
     The present invention also provides pharmaceutical compositions, which comprise compounds of the present invention formulated together with one or more non-toxic pharmaceutically acceptable carriers. The pharmaceutical compositions may be specially formulated for oral administration in solid or liquid form or for parenteral injection. 
     The term “parenteral”, as used herein, refers to modes of administration, which include intravenous, intramuscular, intraperitoneal, subcutaneous and infusion. 
     Solid dosage forms for oral administration include capsules, tablets, pills, powders and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier. 
     Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules. 
     The compounds of the present invention may be administered alone or mixed with other anticancer agents. Among the possible combinations, there may be mentioned
         alkylating agents and in particular cyclophosphamide, melphalan, ifosfamide, chlorambucil, busulfan, thiotepa, prednimustine, carmustine, lomustine, semustine, streptozotocin, decarbazine, temozolomide, procarbazine and hexamethylmelamine   platinum derivatives such as in particular cisplatin, carboplatin or oxaliplatin,   antibiotic agents such as in particular bleomycin, mitomycin, dactinomycin,   antimicrotubule agents such as in particular vinblastine, vincristine, vindesine, vinorelbine, taxoids (paclitaxel and docetaxel),   anthracyclines such as in particular doxorubicin, daunorubicin, idarubicin, epirubicin, mitoxantrone, losoxantrone,   group I and II topoisomerases such as etoposide, teniposide, amsacrine, irinotecan, topotecan and tomudex,   fluoropyrimidines such as 5-fluorouracil, UFT, floxuridine,   cytidine analogues such as 5-azacytidine, cytarabine, gemcitabine, 6-mercaptomurine, 6-thioguanine,   adenosine analogues such as pentostatin, cytarabine or fludarabine phosphate,   methotrexate and folinic acid,   various enzymes and compounds such as L-asparaginase, hydroxyurea, trans-retinoic acid, suramine, dexrazoxane, amifostine, herceptin as well as oestrogenic and androgenic hormones.       

     It is also possible to combine a radiation treatment with the compounds of the present invention. This treatment may be administered simultaneously, separately or sequentially. The treatment will be adapted to the patient to be treated by the practitioner. 
     The invention will be more fully described by the following examples, which must not be considered as a limitation of the invention. 
     EXAMPLES 
     Method for Analytical Determination 
     Liquid Chromatography Coupled to Mass Spectrometry (LC/MS) Analysis LC/MS analyses were conducted on a Micromass instrument model LCT linked to an HP 1100 model instrument. Compound abundance was detected using an HP G1315A (model) photodiode array detector in the 200–600 nm wavelength range and a Sedex 65 (model) evaporative light scattering detector. Mass spectra were acquired in the 160 to 2000 amu range. Data were analysed using the Micromass MassLynx software. Separation were carried out on a Hypersil Highpurity C18, 5 μm particle size column (50×4.6 mm) eluted by a linear gradient of 10 to 90% acetonitrile containing 0.05% (v/v) trifluoroacetic acid (TFA) in water containing 0.05% (v/v) TFA in 6.50 min at a flow rate of 1 ml/min. 
     Method for Purification 
     LC/MS Triggered Purification 
     Compounds were purified by LC/MS using a Waters FractionLynx system composed of a Waters model 600 gradient pump, a Waters model 515 regeneration pump, a Waters Reagent Manager make-up pump, a Waters model 2700 sample manager autoinjector, two Rheodyne model LabPro switches, a Waters model 996 photodiode array detector, a Waters model ZMD mass spectrometer and a Gilson model 204 fraction collector. The Waters FractionLynx software controlled the instrument. Separation were conducted alternatively on two Waters Symmetry columns (C 18 , 5 μM, 19×50 mm, catalogue number 186000210), one column was under regeneration by a 95/5 (v/v) water/acetonitrile mixture containing 0.07% TFA (v/v) while the other one is separating. Columns were eluted by a linear gradient of acetonitrile containing 0.07% (v/v) TFA in water containing 0.07% (v/v) TFA, from 5 to 95% (v/v) in 8 min and 2 min at 95% acetonitrile containing 0.07% (v/v) TFA, at a flow rate of 10 ml/min. At the output of the separating column the flow was split to the 1/1000 ratio using a LC Packing AccuRate splitter; 1/1000 of the flow was mixed with methanol (0.5 ml/min. flow rate) and sent to the detectors, this flow was split again ¾ of the flow was sent to the photodiode array detector and ¼ to the mass spectrometer; the rest of the output of the column (999/1000) was sent to the fraction collector were flow was directed normally to waste unless expected mass signal was detected by the FractionLynx software. The FractionLynx software was supplied with molecular formulas of expected compounds and triggered the collection of compounds when mass signal corresponding to [M+H] +  and [M+Na] +  are detected. In certain cases (depending on analytical LC/MS result, when [M+2H] ++  was detected as an intense ion) the FractionLynx software was additionally supplied with calculated half molecular weight (MW/2), in these conditions collection was also triggered when mass signal corresponding to [M+2H] ++  and [M+Na+H] ++  are detected. Compounds were collected in tarred glass tubes. After collection, solvent was evaporated in a Jouan model RC 10.10 centrifuge evaporator or a Genevac model HT8 centrifuge evaporator and the amount of compound was determined by weighing of the tubes after solvent evaporation. 
     Method of Preparation of Compounds of the Invention 
     2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene (melting point 161° C.), the starting material, can be prepared according to U.S. Pat. No. 3,996,368. 
     Example 1 
     Preparation of Methyl-5-(4-[2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     step 1: 15.6 g of 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene were combined with 25 ml ethanolamine in 100 ml ethyl glycol in a round bottom flask. The reaction mixture was heated to reflux for 90 min and then cooled on ice. Reaction mixture was then diluted with 250 ml of 2N aqueous HCl, the compound precipitated and was filtered off with suction. The preciptate was the washed with water and dried, yielding 15.5 g of 2-amino-5-(4-[2-hydroxyethyl]aminophenylsulfonyloxy)nitrobenzene (melting point 180° C.). 
     step 2: 15.5 g of 2-amino-5-(4-[2-hydroxyethyl]aminophenylsulfonyloxy)nitrobenzene in 75 ml of methanol and 75 ml of dimethylformamide are hydrogenated under atmospheric pressure with a catalytic amount of Raney Nickel (method A). After hydrogen uptake is complete, the catalyst was filtered off with suction, washed with methanol and the filtrate is concentred under reduced pressure 
     step 3: concentrated filtrate of step 2 was taken up in 150 ml methanol and 30 ml of glacial acetic acid, 10.3 g of 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea was added and reaction mixture was heated to reflux with stirring for 3 hours. Solvents were then evaporated under reduced pressure, concentrate was then dissolved in hot ethylacetate, crystallized by cooling and washed with ethylacetate. Compound was then solubilized in 250 ml refluxing methanol, crystallized by cooling and washed with methanol and dried yielding 7.4 g of the title compound. (Melting point 170° C., LC/MS analysis: retention time=2.8 min., mass spectrum: 407.24, [M+H] + ) 
     Example 2 
     Preparation of Methyl-5-(4-[4-hydroxbutyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     step 1: 19.7 g of 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene were combined with 20 g butanolamine in 200 ml N-methylpyrrolidinone in a round bottom flask. The reaction mixture was heated to reflux for 120 min and then solvent was evaporated under reduced pressure. Concentrate was then solubilized with ethylacetate and extracted with 2N aqueous HCl and water and then dried over sodium sulfate and dried under reduced pressure. The concentrate was recrystallized in isopropanol, filtered under suction, washed with isopropanol and dried, yielding 13.1 g of 2-amino-5-(4-[4-hydroxbuyl]aminophenylsulfonyloxy)nitrobenzene (melting point 105° C.). 
     step 2: 13.1 g of 2-amino-5-(4-[4-hydroxbutyl]aminophenylsulfonyloxy)nitrobenzene in 75 ml of methanol and 75 ml of dimethylformamide are hydrogenated under atmospheric pressure with a catalytic amount of Raney Nickel (Method A). After hydrogen uptake is complete, the catalyst was filtered off with suction, washed with methanol and the filtrate is concentred under reduced pressure. 
     step 3: concentrated filtrate of step 2 was taken up in 100 ml methanol and 20 ml of glacial acetic acid, 8.2 g of 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea was added and reaction mixture was heated to reflux with stirring for 3 hours. Solvents were then evaporated under reduced pressure, concentrate washed with 2N aqueous ammonia, water and dried. Concentrate was then dissolved in hot ethyl acetate, crystallized by cooling and washed with ethyl acetate. Compound was then solubilized in refluxing methanol, crystallized by cooling and washed with methanol and dried yielding 6.3 g of the title compound. (Melting point 180° C., LC/MS analysis: retention time=2.9 min., mass spectrum: 435.29, [M+H] + ) 
     Example 3 
     Preparation of Methyl-5-(4-[2-methoxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     step 1: 15.6 g of 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene were combined with 35 ml methoxyethylamine in 100 ml dioxane in a round bottom flask. The reaction mixture was heated to reflux for 8 hours and then cooled to 40° C. and extracted two times with 250 ml water. Concentrate was solubilized with ethyl acetate and extracted with 2N aqueous HCl and water, the organic phase was then dried under reduced pressure, yielding 19.2 g of 2-amino-5-(4-[2-methoxyethyl]aminophenylsulfonyloxy)nitrobenzene (melting point 105° C.). 
     step 2: 18.2 g of 2-amino-5-(4-[2-methoxyethyl]aminophenylsulfonyloxy)nitrobenzene in 75 ml of methanol and 75 ml of dimethylformamide are hydrogenated under atmospheric pressure with a catalytic amount of Raney Nickel (Method A). After hydrogen uptake is complete, the catalyst was filtered off with suction, washed with methanol and the filtrate is concentred under reduced pressure. 
     step 3: concentrated filtrate of step 2 was taken up in 150 ml methanol and 25 ml of glacial acetic acid, 12.3 g of 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea was added and reaction mixture was heated to reflux with stirring for 3 hours. Solvents were then evaporated under reduced pressure, and concentrate was crystallized with methanol saturated with ammonia, washed with water, methanol and dried, yielding 12 g of the title compound. (Melting point 155° C., LC/MS analysis: retention time=3.1 min., mass spectrum: 421.25, [M+H] + ). 
     Example 4 
     Preparation of Methyl-5-(4-[1-imidazolyl]-phenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     step 1: 15.6 g of 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene were combined with 20.7 g imidazole in 100 ml dimethylformamide in a round bottom flask. The reaction mixture was heated to reflux for 3 hours and then cooled to room temperature. Reaction mixture was then precipitated by addition of water filtered and precipitate was washed with water and dried. Residue was resolubilized in hot ethyl glycol, crystallized by cooling and the crystals were washed with methanol and dried, yielding 10.4 g of 2-amino-5-(4-[1-imidazolyl]-phenylsulfonyloxy)nitro-benzene (melting point 209° C.). 
     step 2: 10.4 g of 2-amino-5-(4-[1-imidazolyl]-phenylsulfonyloxy)nitrobenzene in 75 ml of methanol and 75 ml of dimethylformamide are hydrogenated under atmospheric pressure with a catalytic amount of Raney Nickel. After hydrogen uptake is complete, the catalyst was filtered off with suction, washed with methanol and the filtrate is concentred under reduced pressure (Method A). Alternatively 5 g of 2-amino-5-(4-[1-imidazolyl]-phenylsulfonyloxy)nitrobenzene in 475 ml of methanol and 25 ml of dimethylformamide are hydrogenated under 5 bars pressure with 10% (w/w) of palladium on carbon at 30° C. during 6 hours (Method B) yielding 4.18 g (91%) of expected product. 
     step 3: concentrated filtrate of step 2 was taken up in 150 ml methanol and 25 ml of glacial acetic acid, 10.3 g of 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea was added and reaction mixture was heated to reflux with stirring for 3 hours. After cooling to room temperature reaction mixture was precipitated by addition of ethyl acetate, filtered by suction and washed by ethyl acetate. Filtrate was then resolubilized with 50 ml dimethylformamide and 250 ml of methanol was added. Mixture crystallised upon cooling and crystals were washed with methanol and dried under reduced pressure, yielding 9.4 g of the title compound. (Melting point 258° C., LC/MS analysis: retention time=2.5 min., mass spectrum: 414.23, [M+H] + ; 382.19 fragmentation of carbamate: loss of methanol, NMR, IR). 
     Example 5 
     Preparation of Methyl-5-(4-[2-pyridylmethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting 2-aminomethylpyridine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=2.6 min., mass spectrum: 454.28, [M+H] + ; 907.53, [2M+H] + ; 422.24, fragmentation of carbamate: loss of methanol). 
     Example 6 
     Preparation of Methyl-5-(4-ethylaminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting ethylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.2 min., mass spectrum: 390.98, [M+H] + ). 
     Example 7 
     Preparation of Methyl-5-(4-[N-Glycinyl]-phenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting glycine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=2.8 min., mass spectrum: 421.21, [M+H] + ). 
     Example 8 
     Preparation of Methyl-5-(4-[1-methyl,2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting 2-aminopropanol with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=2.9 min., mass spectrum: 421.27, [M+H] + ). 
     Example 9 
     Preparation of Methyl-5-(4-[2-methyl,2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting 1-methyl-2-aminoethanol with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=2.9 min., mass spectrum: 421.27, [M+H] + ). 
     Example 10 
     Preparation of Methyl-5-(4-isopropylaminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting isopropylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.4 min., mass spectrum: 405.27, [M+H] + ). 
     Example 11 
     Preparation of Methyl-5-(4-[1-ethyl, 2-hydroxyethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting 2-aminobutanol with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.0 min., mass spectrum: 435.30, [M+H] + ). 
     Example 12 
     Preparation of Methyl-5-(4-butylaminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting butylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.6 min., mass spectrum: 419.25, [M+H] + ). 
     Example 13 
     Preparation of Methyl-5-(4-[3-methoxypropyl]aminophenyl-sulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting 3-methoxypropanolamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.2 min., mass spectrum: 435.27, [M+H] + ). 
     Example 14 
     Preparation of Methyl-5-(4-methylaminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting methylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.0 min., mass spectrum: 377.22, [M+H] + ). 
     Example 15 
     Preparation of Methyl-5-(4-[2-sulfonylethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting 2-aminoethanesulfonic acid with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=2.6 min., mass spectrum: 471.19, [M+H] + ; 941.41, [2M+H] + ). 
     Example 16 
     Preparation of Methyl-5-(4-aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting ammonia with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=2.9 min., mass spectrum: 363.19, [M+H] + ). 
     Example 17 
     Preparation of Methyl-5-(4-[2-diethylaminoethyl]aminophenyl-sulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting 2-diethylaminoethylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=2.6 min., mass spectrum: 462.34, [M+H] + ; 923.65, [2M+H] + ; 430.30, fragmentation of carbamate: loss of methanol). 
     Example 18 
     Preparation of Methyl-5-(4-[1-tetrathydrofurylmethyl]aminophenyl-sulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting tetrahydrofurfurylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.2 min., mass spectrum: 447.24, [M+H] + ). 
     Example 19 
     Preparation of Methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting cyclopentylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.6 min., mass spectrum: 431.29, [M+H] + ). 
     Example 20 
     Preparation of Methyl-5-(4-[2-phenylethyl]aminophenylsulfonyloxy)benzimidazole-2-carbamate 
     
       
                 
         
             
             
         
      
     
     In a similar manner to examples 1 to 4, title compound was obtained by reacting phenethylamine with 2-amino-5-(4-fluorophenylsulfonyloxy)nitrobenzene at step 1 of the procedure described above and using nitro group reduction method A. (LC/MS analysis: retention time=3.6 min., mass spectrum: 467.26, [M+H] + ). 
     Example 21 
     Preparation of 5-(4-[1-imidazolyl]-phenylsulfonyloxy)-1H-benzimidazole-2-ylamine: an intermediate for amide product synthesis 
     
       
                 
         
             
             
         
      
     
     For step 1 and 2, intermediate of title compound is obtained in similar manner to step 1 end 2 of example 4. 
     step 3: 8 g of step 2 compound were taken up in 128 ml methanol and 21.6 ml acetic acid in a 250 ml round bottom flask. Mixture was heated to reflux and 9.13 g of 1,3-bis(tert-butoxycarbonyl)-2-methyl-2-thiopseudourea was added. Reaction mixture was heated to reflux with stirring for 4 hours. Solid was obtained by cooling to 0° C. for one hour and washed with ethyl acetate, triturated and dried on a glass frit yielding 7.55 g compound. 
     step 4: Compound of step 3 was taken up in 80 ml dichloromethane and 40 ml trifluoroacetic acid. Reaction mixture was stirred for 4 hours at room temperature. Solvents were evaporated under reduced pressure. Concentrated filtrate was taken in 75 ml water and 50 ml of sodium carbonate aqueous solution (10% w/w). Precipitate obtained was washed with dichloromethane and dried on a glass frit yielding 5.3 g title compound. 
     Example 22 
     Preparation of 5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-ylamine: an intermediate for amide product synthesis 
     
       
                 
         
             
             
         
      
     
     For step 1 and 2, intermediate of title compound is obtained in similar manner to step 1 end 2 of example 19. 
     For step 3 and 4, title compound is obtained in similar manner to example 21. 
     Example 23 
     Preparation of N-[5-(4-Imidazol-1-yl-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-succinamic acid methyl ester 
     
       
                 
         
             
             
         
      
     
     step 1: 8.9 mg of succinamic acid methyl ester, 25 mg of 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) and 12 μl diisopropyl-ethylamine were taken up in 0.4 ml dimethylformamide. Reaction mixture was stirred at room temperature for one hour and 5-(4-[1-imidazolyl]-phenylsulfonyloxy)-1H-benzimidazole-2-ylamine was added in 0.2 ml dimethylformamide. Reaction mixture was then stirred at room temperature for 24 hours. Solvent was evaporated in a Jouan model RC 10.10 centrifuge evaporator and title compound was solubilised in 0.5 ml dimethylsulfoxide for LCMS trigged purification yielding 3.9 mg of N-[5-(4-[1-imidazolyl]-phenylsulfonyloxy)-1H-benzimidazole-2-yl]-succinamic-acid-methyl ester. (LC/MS analysis: retention time=2.70 min., mass spectrum: 470.34, [M+H] + ). 
     Example 24 
     Preparation of 4-Cyclopentylamino-benzenesulfonic acid 2-(2-tert-butoxycarbonylamino-acetylamino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     step 1: 11.3 mg of N-(tert-butoxycarbonyl)glycine, 25 mg HBTU and 12 μl diisopropylethylamine were taken up in 0.4 ml dimethylformamide. Reaction mixture was stirred at room temperature for one hour and 5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-ylamine was added in 0.2 ml dimethylformamide. Reaction mixture was then stirred at room temperature for 24 hours. Solvent was evaporated in a Jouan model RC 10.10 centrifuge evaporator and title compound was solubilised in 0.5 ml dimethylsulfoxide for LCMS trigged purification yielding 2.4 mg of N-[5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-yl]-tert-butoxycarbonylglycineamid. (LC/MS analysis: retention time=3.87 min., mass spectrum: 530.38, [M+H] + ). 
     Example 25 
     Preparation of N-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-succinamic acid methyl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 24, title compound was obtained by reacting succinamic acid methyl ester with N-5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-ylamine (LC/MS analysis: retention time=3.72 min., mass spectrum: 487.34, [M+H] + ). 
     Example 26 
     Preparation of 4-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-ylcarbamoyl]-butyric acid methyl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 24, title compound was obtained by reacting butyric acid methylester with N-5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-ylamine. (LC/MS analysis: retention time=3.75 min., mass spectrum: 501.36, [M+H] + ). 
     Example 27 
     Preparation of 4-Cyclopentylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 24, title compound was obtained by reacting cyclopropane carboxylic acid with 5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-ylamine. (LC/MS analysis: retention time=3.76 min., mass spectrum: 441.36, [M+H] + ). 
     Example 28 
     Preparation of 4-Cyclopentylamino-benzenesulfonic acid 2-(2-methoxy-acetylamino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 24, title compound was obtained by reacting methoxyaceticacid with 5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-ylamine. (LC/MS analysis: retention time=3.66 min., mass spectrum: 445.34, [M+H] + ). 
     Example 29 
     Preparation of 4-Cyclopentylamino-benzenesulfonic acid 2-(2-dimethylamino-acetylamino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 24, title compound was obtained by reacting N,N-dimethylglycine with 5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-ylamine. (LC/MS analysis: retention time=3.36 min., mass spectrum: 458.36, [M+H] + ). 
     Example 30 
     N-[5-(4-[imidazolyl]-phenylsulfonyloxy)-1H-benzimidazole-2-yl]-methylurea 
     
       
                 
         
             
             
         
      
     
     10 mg of methyl-5-(4-[imidazolyl]-phenylsulfoxy)benzimidazole-2-carbamate (example 4) were combined with 50 μl methylamine (2.0 M in tetrahydrofuran) and 5 μl 1,8-Diazabicyclo[5.4.0]undec-7-ene in 2 ml N-methylpyrrolidone/tetrahydrofuran (1/1) in a 24 well Inox plate for high pressure reaction. The reaction mixture was put under a 10 Bars argon pressure and then heated to 80° C. for 4 hours, and then cooled at room temperature. Compounds were put in an assay tube and tetrahydrofuran was evaporated under reduced pressure and compound in N-methylpyrrolidone were directly purified by preparative LCMS under conditions described above. After purification, solution was dry-concentrated in a JOUAN RC1010 evaporator. (LC/MS analysis: retention time=2.23 min., mass spectrum: 413.23, [M+H] + ). 
     Example 31 
     N-[5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-yl]-methylurea 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 30, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with methylamine (2.0 M in tetrahydrofuran). (LC/MS analysis: retention time=3.30 min., mass spectrum: 430.27, [M+H] + ). 
     Example 32 
     N-[5-(4-cyclopentylaminophenylsulfonyloxy)-1H-benzimidazole-2-yl]-dimethylurea 
     
       
                 
         
             
             
         
      
     
     Title compound was obtained by reacting 10 mg of methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 50 μl dimethylamine (2.0 M in tetrahydrofuran) and 5 μl 1,8-diazabicyclo[5.4.0]undec-7-ene in 2 ml dimethylformamide in a 24 well Inox plate for high pressure reaction. The reaction mixture was put under a 10 Bars argon pressure and then heated to 80° C. for 4 hours, and then cooled at room temperature. Compounds were put in an assay tube and dimethylformamide was evaporated in a JOUAN RC1010 evaporator. Compound was diluted in 0.5 ml dimethylsulfoxide for LC/MS trigged purification yielding 9 mg of the title compound (LC/MS analysis: retention time=3.35 min., mass spectrum: 444.29, [M+H] + ). 
     Example 33 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-cyclopropyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     10 mg of methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) were combined with 25 μl cyclopropylamine and 10 μl 1,8-diazabicyclo[5.4.0]undec-7-ene in 2 ml N-methylpyrrolidone/tetrahydrofuran (0.8/1.2) in a 24 well Inox plate for high pressure reaction. The reaction mixture was put under a 10 Bars argon pressure and then heated to 60° C. for 40 hours, and then cooled to room temperature. Compounds were put in an assay tube, tetrahydrofuran was evaporated under reduced pressure and compound in N-methylpyrrolidone was directly purified by LC/MS trigged purification yielding 8.7 mg title compound. (LC/MS analysis: retention time=3.66 min., mass spectrum: 456.36, [M+H] + ). 
     Example 34 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-isopropyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with isopropylamine. (LC/MS analysis: retention time=3.78 min., mass spectrum: 458.36, [M+H] + ). 
     Example 35 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-butyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with butylamine. (LC/MS analysis: retention time=3.90 min., mass spectrum: 472.39, [M+H] + ). 
     Example 36 
     4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 30, title compound was obtained by reacting methyl-5-(4-[imidazolyl]-phenylsulfoxy)benzimidazole-2-carbamate (example 4) with 2-fluoro-aniline. (LC/MS analysis: retention time=3.03 min., mass spectrum: 493.28, [M+H] + ). 
     Example 37 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 2-fluoro-aniline. (LC/MS analysis: retention time=3.99 min., mass spectrum: 510.32, [M+H] + ). 
     Example 38 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with m-anisidine (LC/MS analysis: retention time=4.02 min., mass spectrum: 522.33, [M+H] + ). 
     Example 39 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-methoxy-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with p-anisidine (LC/MS analysis: retention time=3.97 min., mass spectrum: 522.34, [M+H] + ). 
     Example 40 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 4-chloroaniline. (LC/MS analysis: retention time=4.20 min., mass spectrum: 526.28, [M+H] + ). 
     Example 41 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-fluoro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 3-fluorobenzylamine. (LC/MS analysis: retention time=3.96 min., mass spectrum: 524.33, [M+H] + ). 
     Example 42 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-chloro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 3-chloroaniline. (LC/MS analysis: retention time=4.21 min., mass spectrum: 526.28, [M+H] + ). 
     Example 43 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-isobutyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with isobutylamine. (LC/MS analysis: retention time=3.88 min., mass spectrum: 472.38, [M+H] + ). 
     Example 44 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-dimethylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with N,N-dimethylethylenediamine. (LC/MS analysis: retention time=3.22 min., mass spectrum: 487.38, [M+H] + ). 
     Example 45 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-ethyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with ethylamine (33% in water). (LC/MS analysis: retention time=3.64 min., mass spectrum: 444.35, [M+H] + ). 
     Example 46 
     { 3 -[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-acetic acid 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with glycine. (LC/MS analysis: retention time=3.48 min., mass spectrum: 474.31, [M+H] + ). 
     Example 47 
     4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-sulfo-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 30, title compound was obtained by reacting methyl-5-(4-[imidazolyl]-phenylsulfoxy)benzimidazole-2-carbamate (example 4) with 2-aminoethanesulfonic acid. (LC/MS analysis: retention time=2.40 min., mass spectrum: 507.21, [M+H] + ). 
     Example 48 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 2-methoxyethylamine. (LC/MS analysis: retention time=3.60 min., mass spectrum: 474.34, [M+H] + ). 
     Example 49 
     4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-dimethylamino-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with N,N-dimethyl-1,4-phenylenediamine. (LC/MS analysis: retention time=3.42 min., mass spectrum: 535.34, [M+H] + ). 
     Example 50 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 2-aminomethylpyridine. (LC/MS analysis: retention time=3.30 min., mass spectrum: 507.33, [M+H] + ). 
     Example 51 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-cyclobutyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with cyclobutylamine. (LC/MS analysis: retention time=3.84 min., mass spectrum: 470.36, [M+H] + ). 
     Example 52 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-pyridin-4-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with 4-(aminomethyl)pyridine. (LC/MS analysis: retention time=3.24 min., mass spectrum: 507.33, [M+H] + ). 
     Example 53 
     4-Cyclopentylamino-benzenesulfonic acid 2-(3-tert-butyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     In a similar manner to example 33, title compound was obtained by reacting methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with tert-butylamine. (LC/MS analysis: retention time=3.93 min., mass spectrum: 472.36, [M+H] + ). 
     Example 54 
     4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-methyl-ureido)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     10 mg of methyl-5-(4-[1-tetrathydrofurylmethyl]aminophenyl-sulfonyloxy) benzimidazole-2-carbamate (example 18) were combined with 50 μl methylamine (2.0 M in tetrahydrofuran) and 5 μl 1,8-diazabicyclo[5.4.0]undec-7-ene in 2 ml N-methylpyrrolidone/tetrahydrofuran (1/1) in a 24 well Inox plate for high pressure reaction. The reaction mixture was put under a 10 Bars argon pressure and then heated to 80° C. for 4 hours, and then cooled at room temperature. Compounds were put in an assay tube and tetrahydrofuran was evaporated under reduce pressure and compound in N-methylpyrrolidone were directly purified by preparative LCMS in conditions described above. After purification, solution were dry-concentrated in a JOUAN RC1010 evaporator. (LC/MS analysis: retention time=2.91 min., mass spectrum: 446.07, [M+H] + ). 
     Example 55 
     4-Fluoro-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     step 1: 10 g of 4-amino-3-nitrophenol in 180 ml of ethanol were hydrogenated under 40 bars pressure at 23° C. temperature with catalytic amound of palladium on carbon. Reaction was performed in an Inox flask for high pressure. After hydrogen uptake was complete, the catalyst was filtered off with suction, washed with methanol and the filtrate was concentred under reduced pressure yielding 8 g of crude 3,4-diaminophenol. 
     Step 2: 5.75 g of 3,4-diaminophenol were combined with 15.5 g of 1,3-bis(tert-butoxycarbonyl)-2-methyl-2-thiopseudourea in 150 ml methanol and 22 ml acetic acid in a round bottom flask. The reaction mixture was heated to reflux with stirring for 3 hours. Solvents were then evaporated under reduce pressure yielding 7.13 g crude (5-hydroxy-1H-benzoimidazol-2-yl)-carbamic acid tert-butyl ester. 
     Step 3: 5.98 g of (5-hydroxy-1H-benzoimidazol-2-yl)-carbamic acid tert-butyl ester were combinated with 4.67 g of 4-fluorobenzenesulfonyl chloride and 6.75 ml of trietylamine in 100 ml acetone. The reaction mixture was stirred at room temperature for 1 hour. Solvents were evaporated under reduced pressure yielding 6.45 g crude 4-fluoro-benzenesulfonic acid 2-tert-butoxycarbonylamino-1H-benzoimidazol-5-yl ester. 
     Step 4: 6.45 g of 4-fluoro-benzenesulfonic acid 2-tert-butoxycarbonylamino-1H-benzoimidazol-5-yl ester were combined with 15 ml trifluoroacetic acid in 60 ml dichloromethane. The reaction mixture was stirred overnight at room temperature. Solvents were evaporated under reduced pressure. The residue was washed with ethyl ether and dried on glass frit yielding 6.58 g of 4-fluoro-benzenesulfonic acid 2-amino-1H-benzoimidazol-5-yl ester trifluoroacetic acid salt. 
     Step 5: 5.53 g of 4-fluoro-benzenesulfonic acid 2-amino-1H-benzoimidazol-5-yl ester trifluoroacetic acid salt were combinated with 1.8 ml of cyclopropanecarbonylchloride and 5 ml triethylamine in 75 ml dichloromethane. Reaction mixture was stirred at room temperature for 1 hour. Solvents were evaporated under reduced pressure. The residue was then taken up in dichloromethane, washed with water and dried with magnesium sulfate. Dichloromethane was evaporated under reduced pressure and precipitate obtained was dried on glass frit yielding 4.88 g of 4-fluoro-benzenesulfonic acid 2-amino-3-cyclopropanecarbonyl-3H-benzoimidazol-5-yl ester. 
     Step 6: 3.27 g of 4-fluoro-benzenesulfonic acid 2-amino-3-cyclopropanecarbonyl-3H-benzoimidazol-5-yl ester; were combinated with 106 mg of 4-(dimethylamino)pyridine in 80 ml acetonitrile. the reaction mixture was heated at 85° C. temperature for 72 hours with stirring. Yellow solution obtained was diluted in dichloromethane, washed with water and dried under magnesium sulfate. Solvents were evaporated under reduced pressure yielding 3.19 g of the title compound. 
     Example 56 
     Preparation of 4-[(1-Ethyl-pyrrolidin-2-ylmethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     Title compound was obtained by reacting 12 mg of 4-fluoro-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester (example 55) with 21 mg of 2-(aminomethyl)-1-ethylpyrrolidine and 50 mg cesium carbonate in 600 μl dimethylsulfoxide. Reaction was performed in a 24 well Inox plate for high pressure. The reaction mixture was put under 10 bars argon pressure and then heated to 110° C. for 50 hours. Cesium carbonate was filtered off and compound in DMSO was directly purified by LCMS triggered purification yielding 10.7 mg title compound. (LC/MS analysis: retention time=2.58 min, mass spectrum: 483.99, [M+H] + . 
     Example 57 
     By using a method similar to that for the preparation of example 30, combining methyl-5-(4-[1-imidazolyl]-phenylsulfonyloxy)benzimidazole-2-carbamate precursor (example 4) with suitable amine were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table. 
     
       
         
               
               
               
               
               
               
             
           
               
                   
               
               
                 n o   
                 Precursor 
                   
                   
                   
                 Retention 
               
               
                 example 
                 example 
                 Amine 
                 compound 
                 [M + H] +   
                 time (min) 
               
               
                   
               
             
             
               
                 57-a 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-(3-phenyl-ureido)-1H-benzoimidazol-5-yl ester 
                 475.23 
                 2.75 
               
               
                   
               
               
                 57-b 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-(3-cyclohexyl-ureido)-1H-benzoimidazol-5-yl ester 
                 481.28 
                 2.76 
               
               
                   
               
               
                 57-c 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-(3-cyclopentyl-ureido)-1H-benzoimidazol-5-yl ester 
                 467.24 
                 2.62 
               
               
                   
               
               
                 57-d 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(3-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 493.22 
                 2.87 
               
               
                   
               
               
                 57-e 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-hydroxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 443.23 
                 2.32 
               
               
                   
               
               
                 57-f 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(4-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 493.22 
                 2.80 
               
               
                   
               
               
                 57-g 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-chloro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 523.25 
                 3.08 
               
               
                   
               
               
                 57-h 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-[3-(2-fluoro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 507.28 
                 3.00 
               
               
                   
               
               
                 57-i 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-[(azetidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester 
                 439.31 
                 2.55 
               
               
                   
               
               
                 57-j 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-(3-pyridin-3-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 490.31 
                 2.39 
               
               
                   
               
               
                 57-k 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-{3-[3-(4-methyl-piperazin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 539.36 
                 2.34 
               
               
                   
               
               
                 57-l 
                 4 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Imidazol-1-yl-benzenesulfonic acid 2-(3-benzyl-ureido)-1H-benzoimidazol-5-yl ester 
                 489.25 
                 2.71 
               
               
                   
               
             
          
         
       
     
     Example 58 
     By using a method similar to that for the preparation of example 33, combining methyl-5-(4-cyclopentylaminophenylsulfonyloxy)benzimidazole-2-carbamate (example 19) with suitable amine were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
               
             
           
               
                   
               
               
                 n o   
                 Precursor 
                   
                   
                   
                 Retention 
               
               
                 example 
                 example 
                 Amine 
                 compound 
                 [M + H] +   
                 time (min) 
               
               
                   
               
             
             
               
                 58-a 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-phenyl-ureido)-1H-benzoimidazol-5-yl ester 
                 492.28 
                 3.77 
               
               
                   
               
               
                 58-b 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-cyclohexyl-ureido)-1H-benzoimidazol-5-yl ester; 
                 498.31 
                 3.79 
               
               
                   
               
               
                 58-c 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-cyclopentyl-ureido)-1H-benzoimidazol-5-yl ester 
                 484.29 
                 3.68 
               
               
                   
               
               
                 58-d 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 510.25 
                 3.87 
               
               
                   
               
               
                 58-e 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-hydroxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 460.28 
                 3.17 
               
               
                   
               
               
                 58-f 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-fluoro-phenyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 510.24 
                 3.80 
               
               
                   
               
               
                 58-g 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-chloro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 540.28 
                 4.05 
               
               
                   
               
               
                 58-h 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-fluoro-benzyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 524.33 
                 3.97 
               
               
                   
               
               
                 58-i 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[(azetidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester 
                 456.37 
                 3.60 
               
               
                   
               
               
                 58-j 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-pyridin-3-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 507.35 
                 3.24 
               
               
                   
               
               
                 58-k 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-{3-[3-(4-methyl-piperazin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 556.39 
                 3.09 
               
               
                   
               
               
                 58-l 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-benzyl-ureido)-1H-benzoimidazol-5-yl ester 
                 506.31 
                 3.70 
               
               
                   
               
               
                 58-m 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-butyric acidmethyl ester 
                 516.31 
                 3.74 
               
               
                   
               
               
                 58-n 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-butyric acidethyl ester 
                 530.30 
                 3.84 
               
               
                   
               
               
                 58-o 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-acetic acidmethyl ester 
                 488.26 
                 3.65 
               
               
                   
               
               
                 58-p 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-imidazol-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 524.30 
                 3.29 
               
               
                   
               
               
                 58-q 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2S-ylcarbamoyl]-pyrrolidine-2-carboxylic acid 
                 514.27 
                 3.56 
               
               
                   
               
               
                 58-r 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2S-yl-carbamoyl]-pyrrolidine-2-carboxylic acid methyl ester 
                 528.27 
                 3.79 
               
               
                   
               
               
                 58-s 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-carbamoylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 473.28 
                 3.38 
               
               
                   
               
               
                 58-t 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl-carbamoyl]-piperidine-4-carboxylic acid ethyl ester 
                 556.29 
                 3.93 
               
               
                   
               
               
                 58-u 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-piperidin-4-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 513.33 
                 3.25 
               
               
                   
               
               
                 58-v 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 529.31 
                 3.27 
               
               
                   
               
               
                 58-w 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-amino-2-methyl-propyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 487.32 
                 3.25 
               
               
                   
               
               
                 58-x 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4trans-hydroxy-cyclohexyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 514.30 
                 3.54 
               
               
                   
               
               
                 58-y 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 474.28 
                 3.46 
               
               
                   
               
               
                 58-z 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(1,1-dimethyl-propyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 486.33 
                 4.08 
               
               
                   
               
               
                 58-aa 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-{3-[2-(2-hydroxy-ethylamino)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 503.30 
                 3.19 
               
               
                   
               
               
                 58-ab 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(4-hydroxy-butyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 488.29 
                 3.49 
               
               
                   
               
               
                 58-ac 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-{3-[3-(2-oxo-pyrrolidin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 541.29 
                 3.54 
               
               
                   
               
               
                 58-ad 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-carbamoyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 487.30 
                 3.40 
               
               
                   
               
               
                 58-ae 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[(2S-carbamoyl-pyrrolidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester 
                 513.30 
                 3.42 
               
               
                   
               
               
                 58-af 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-{3-[2-(2-hydroxy-ethoxy)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 504.30 
                 3.46 
               
               
                   
               
               
                 58-ag 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(3-pyrrolidin-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 527.35 
                 3.33 
               
               
                   
               
               
                 58-ah 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(1-ethyl-pyrrolidin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 527.35 
                 3.30 
               
               
                   
               
               
                 58-ai 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-pyrrolidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 513.34 
                 3.29 
               
               
                   
               
               
                 58-aj 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 527.35 
                 3.33 
               
               
                   
               
               
                 58-ak 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-hydroxy-1-methyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 474.30 
                 3.51 
               
               
                   
               
               
                 58-al 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-isopropylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 501.34 
                 3.31 
               
               
                   
               
               
                 58-am 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[3-(2-diethylamino-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 515.34 
                 3.31 
               
               
                   
               
               
                 58-an 
                 19 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2-{3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-yl]-ureido}-3S-hydroxy-propionic acid methyl ester 
                 518.27 
                 3.55 
               
               
                   
               
             
          
         
       
     
     Example 59 
     By using a method similar to that for the preparation of example 54, combining methyl-5-(4-[1-tetrathydrofurylmethyl]aminophenyl-sulfonyloxy)benzimidazole-2-carbamate (example 18) with suitable amine were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
               
             
           
               
                   
               
               
                 n o   
                 Precursor 
                   
                   
                   
                 Retention 
               
               
                 example 
                 example 
                 Amine 
                 compound 
                 [M + H] +   
                 time (min) 
               
               
                   
               
             
             
               
                 59-a 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-carbamoylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 489.15 
                 2.78 
               
               
                   
               
               
                 59-b 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-hydroxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 476.15 
                 2.91 
               
               
                   
               
               
                 59-c 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 490.18 
                 2.89 
               
               
                   
               
               
                 59-d 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(4-hydroxy-butyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 504.19 
                 3.44 
               
               
                   
               
               
                 59-e 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-[3-(2-methoxy-1-methyl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 504.19 
                 3.11 
               
               
                   
               
               
                 59-f 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-[3-(1-ethyl-pyrrolidin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 543.24 
                 2.89 
               
               
                   
               
               
                 59-g 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-[3-(2-pyrrolidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 529.22 
                 2.66 
               
               
                   
               
               
                 59-h 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-[3-(3-pyrrolidin-1-yl-propyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 543.25 
                 2.73 
               
               
                   
               
               
                 59-i 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 545.23 
                 2.61 
               
               
                   
               
               
                 59-j 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-(3-ethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 460.17 
                 3.08 
               
               
                   
               
               
                 59-k 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 523.07 
                 2.70 
               
               
                   
               
               
                 59-l 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-3-ylmethyl-ureido)-1H-benzoimidazol-5-yl ester 
                 523.19 
                 2.64 
               
               
                   
               
               
                 59-m 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-{3-[3-(4-methyl-piperazin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 572.27 
                 2.60 
               
               
                   
               
               
                 59-n 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-sulfo-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 540.13 
                 2.81 
               
               
                   
               
               
                 59-o 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-cyclobutyl-ureido)-1H-benzoimidazol-5-yl ester 
                 486.19 
                 3.28 
               
               
                   
               
               
                 59-p 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonic acid 2-{3-[3-(2-oxo-pyrrolidin-1-yl)-propyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 557.23 
                 2.94 
               
               
                   
               
               
                 59-q 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-{3-[2-(2-hydroxy-ethylamino)-ethyl]-ureido}-1H-benzoimidazol-5-yl ester 
                 519.21 
                 2.64 
               
               
                   
               
               
                 59-r 
                 18 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2-ylmethyl)-amino]-benzenesulfonicacid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 543.24 
                 2.74 
               
               
                   
               
             
          
         
       
     
     Example 60 
     By using a method similar to that for the preparation of example 56, combining 4-fluoro-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester (example 55) with suitable amine were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                 n o   
                 Precursor 
                   
                   
                   
                 Retention 
               
               
                 example 
                 example 
                 Amine 
                 compound 
                 [M + H] +   
                 time (min) 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 60-a  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzylamino-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 436.3 
                 3.68 
               
               
                   
               
               
                 60-b  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Methylamino-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 387.3 
                 3.18 
               
               
                   
               
               
                 60-c  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 417.3 
                 2.98 
               
               
                   
               
               
                 60-d  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazo1-5-yl ester 
                 431.35 
                 3.04 
               
               
                   
               
               
                 60-e  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Hydroxy-butylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 445.35 
                 3.10 
               
               
                   
               
               
                 60-f  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-1-methyl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 445.35 
                 3.41 
               
               
                   
               
               
                 60-g  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Pyrrolidin-1-yl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 470.37 
                 2.80 
               
               
                   
               
               
                 60-h  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-Hydroxymethyl-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 445.34 
                 3.21 
               
               
                   
               
               
                 60-i  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 431.34 
                 3.08 
               
               
                   
               
               
                 60-j  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Piperidin-1-yl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 484.38 
                 2.85 
               
               
                   
               
               
                 60-k  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Pyrrolidin-1-yl-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 484.37 
                 2.85 
               
               
                   
               
               
                 60-l  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-2,2-dimethyl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 459.34 
                 3.30 
               
               
                   
               
               
                 60-m  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-3-yl-methyl)-amino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 464.30 
                 2.77 
               
               
                   
               
               
                 60-n  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[3-(4-Methyl-piperazin-1-yl)-propylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 513.38 
                 2.66 
               
               
                   
               
               
                 60-o  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 493.32 
                 3.74 
               
               
                   
               
               
                 60-p  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Hydroxy-cyclohexylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 471.36 
                 3.11 
               
               
                   
               
               
                 60-q  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Diethylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 472.37 
                 2.82 
               
               
                   
               
               
                 60-r  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1S-Hydroxymethyl-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 445.32 
                 3.19 
               
               
                   
               
               
                 60-s  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Ethylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 444.34 
                 2.75 
               
               
                   
               
               
                 60-t  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Diisopropylamino-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 500.38 
                 2.91 
               
               
                   
               
               
                 60-u  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 486.34 
                 2.76 
               
               
                   
               
               
                 60-v  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-Aza-bicyclo[2.2.2]-oct-3-ylamino)-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 482.35 
                 2.82 
               
               
                   
               
               
                 60-w  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Phenylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 492.34 
                 3.34 
               
               
                   
               
               
                 60-x  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-Benzyl-pyrrolidin-3-ylamino)-benzenesulonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 532.35 
                 3.05 
               
               
                   
               
               
                 60-y  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2R-Carbamoyl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 470.32 
                 3.00 
               
               
                   
               
               
                 60-z  
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Dimethylamino-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester; 
                 458.36 
                 2.79 
               
               
                   
               
               
                 60-aa 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Piperazin-1-yl-ethylamino)-benzene-sulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 485.37 
                 2.63 
               
               
                   
               
               
                 60-ab 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Carbamoyl-cyclohexylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 498.34 
                 3.24 
               
               
                   
               
               
                 60-ac 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Acetylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 458.32 
                 2.95 
               
               
                   
               
               
                 60-ad 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(2-Amino-ethylamino)-ethylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 459.34 
                 2.60 
               
               
                   
               
               
                 60-ae 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[3-(2-Oxo-pyrrolidin-1-yl)-propylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 498.34 
                 3.14 
               
               
                   
               
               
                 60-af 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(1H-Imidazol-4-yl)-ethylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 467.31 
                 2.79 
               
               
                   
               
               
                 60-ag 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 464.31 
                 2.80 
               
               
                   
               
               
                 60-ah 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclobutylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 427.31 
                 3.63 
               
               
                   
               
               
                 60-ai 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(2-Hydroxy-ethoxy)-ethylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 461.34 
                 3.01 
               
               
                   
               
               
                 60-aj 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,3-Dihydroxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 447.31 
                 2.84 
               
               
                   
               
               
                 60-ak 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Imidazol-1-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 467.32 
                 2.78 
               
               
                   
               
               
                 60-al 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(2-Hydroxy-ethylamino)-ethylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 460.34 
                 2.70 
               
               
                   
               
               
                 60-am 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 431.32 
                 3.27 
               
               
                   
               
               
                 60-an 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Dimethylamino-1-methyl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 458.36 
                 2.79 
               
               
                   
               
               
                 60-ao 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Pyrrolidin-3-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 442.35 
                 2.75 
               
               
                   
               
               
                 60-ap 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(1H-Indol-3-yl)-ethylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 516.32 
                 3.75 
               
               
                   
               
               
                 60-aq 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Dimethylamino-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 444.35 
                 2.74 
               
               
                   
               
               
                 60-ar 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Phenoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 493.32 
                 3.78 
               
               
                   
               
               
                 60-as 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Bicyclo[2.2.1]hept-2R-ylamino)-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 467.35 
                 3.95 
               
               
                   
               
               
                 60-at 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 430.35 
                 2.74 
               
               
                   
               
               
                 60-au 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Propylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 458.36 
                 2.84 
               
               
                   
               
               
                 60-av 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-Methyl-2-phenoxy-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 507.33 
                 3.89 
               
               
                   
               
               
                 60-aw 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Piperidin-4-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 470.36 
                 2.84 
               
               
                   
               
               
                 60-ax 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Methoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 493.31 
                 3.68 
               
               
                   
               
               
                 60-ay 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1H-Benzoimidazol-5-ylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 489.29 
                 2.80 
               
               
                   
               
               
                 60-az 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Methoxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 445.10 
                 3.17 
               
               
                   
               
               
                 60-ba 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,2-Dimethoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 461.11 
                 3.20 
               
               
                   
               
               
                 60-bb 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Dimethylamino-phenylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 492.12 
                 2.87 
               
               
                   
               
               
                 60-bc 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Methoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 493.10 
                 3.69 
               
               
                   
               
               
                 60-bd 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Pyrrolidin-1-yl-butylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 498.16 
                 2.64 
               
               
                   
               
               
                 60-be 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,3-Dimethoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 523.11 
                 3.80 
               
               
                   
               
               
                 60-bf 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Prop-2-ynylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 411.07 
                 3.15 
               
               
                   
               
               
                 60-bg 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[4-(2-Hydroxy-ethyl)-piperazin-1-yl]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 464.09 
                 2.48 
               
               
                   
               
               
                 60-bh 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-4-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 464.09 
                 2.48 
               
               
                   
               
               
                 60-bi 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(Ethyl-m-tolyl-amino)-ethylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 534.18 
                 3.10 
               
               
                   
               
               
                 60-bj 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-cyclohexylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 471.13 
                 3.25 
               
               
                   
               
               
                 60-bk 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Dimethylamino-2,2-dimethyl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 486.18 
                 2.64 
               
               
                   
               
               
                 60-bl 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[3-(2-Hydroxy-ethylamino)-propylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 474.14 
                 2.45 
               
               
                   
               
               
                 60-bm 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2RS-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester; 
                 456.80 
                 3.15 
               
               
                   
               
               
                 60-bn 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-furan-2R-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester; 
                 456.87 
                 3.23 
               
               
                   
               
               
                 60-bo 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 (Tetrahydro-furan-2S-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester; 
                 456.88 
                 3.48 
               
               
                   
               
               
                 60-bp 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Butylamino-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 471.92 
                 2.80 
               
               
                   
               
               
                 60-bq 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Methylamino-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 443.90 
                 2.54 
               
               
                   
               
               
                 60-br 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1S,2-Dicarbamoyl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 486.90 
                 2.60 
               
               
                   
               
               
                 60-bs 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-3R-hydroxy-propionic acid methyl ester 
                 474.83 
                 2.81 
               
               
                   
               
               
                 60-bt 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Carbamoyl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 443.86 
                 2.71 
               
               
                   
               
               
                 60-bu 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Methoxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 444.89 
                 3.15 
               
               
                   
               
               
                 60-bv 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3,4,5-Trimethoxy-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 552.85 
                 3.46 
               
               
                   
               
               
                 60-bw 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Carbamoylmethyl-amino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 429.88 
                 2.85 
               
               
                   
               
               
                 60-bx 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperidine-4-carboxylicacid ethyl ester 
                 512.87 
                 3.81 
               
               
                   
               
               
                 60-by 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Amino-2-methyl-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 443.92 
                 2.48 
               
               
                   
               
               
                 60-bz 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-propionic acid methyl ester 
                 458.88 
                 3.13 
               
               
                   
               
               
                 60-ca 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Morpholin-4-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 499.91 
                 2.56 
               
               
                   
               
               
                 60-cb 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(5-Hydroxy-pentylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 458.93 
                 3.08 
               
               
                   
               
               
                 60-cc 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(5S-Amino-2,2,4S-trimethyl-cyclopentylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 511.94 
                 3.24 
               
               
                   
               
               
                 60-cd 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxymethyl-phenylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 2.98 
                 430.91 
               
               
                   
               
               
                 60-ce 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Ethoxy-phenylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 493.17 
                 3.97 
               
               
                   
               
               
                 60-cf 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Ethylamino-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 401.23 
                 3.42 
               
               
                   
               
               
                 60-cg 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Sulfo-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 481.00 
                 2.87 
               
               
                   
               
               
                 60-ch 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-piperidine-1-carboxylicacid ethyl ester 
                 528.06 
                 3.77 
               
               
                   
               
               
                 60-ci 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-({4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-methyl)-benzoic acid 
                 507.00 
                 3.30 
               
               
                   
               
               
                 60-cj 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(1-Carbamimidoyl-piperidin-4-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 512.10 
                 2.73 
               
               
                   
               
               
                 60-ck 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperazine-1-carboxylicacid tert-butyl ester 
                 542.07 
                 3.85 
               
               
                   
               
               
                 60-cl 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-3-phenyl-propionic acid 
                 521.03 
                 3.38 
               
               
                   
               
               
                 60-cm 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Piperidin-1-yl-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 441.07 
                 3.76 
               
               
                   
               
               
                 60-cn 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-Methyl-4-oxo-imidazolidin-2-ylideneamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 468.93 
                 2.45 
               
               
                   
               
               
                 60-co 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Methyl-piperazin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 456.21 
                 2.94 
               
               
                   
               
               
                 60-cp 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-pyrrolidin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 442.95 
                 3.05 
               
               
                   
               
               
                 60-cq 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Cyclopropylmethyl-amino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 426.97 
                 3.61 
               
               
                   
               
               
                 60-cr 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(2-Dimethylamino-ethyl)-methyl-amino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 457.98 
                 2.51 
               
               
                   
               
               
                 60-cs 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Isobutylamino-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 428.99 
                 3.96 
               
               
                   
               
               
                 60-ct 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[Ethyl-(2-hydroxy-ethyl)-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 444.97 
                 2.96 
               
               
                   
               
               
                 60-cu 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-1-hydroxymethyl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 446.93 
                 2.61 
               
               
                   
               
               
                 60-cv 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Propylamino-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 414.96 
                 3.45 
               
               
                   
               
               
                 60-cw 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopropylamino-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 412.95 
                 3.38 
               
               
                   
               
               
                 60-cx 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Morpholin-4-yl-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 442.95 
                 3.24 
               
               
                   
               
               
                 60-cy 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(1-Methyl-pyrrolidin-2-yl)-ethylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 483.99 
                 2.63 
               
               
                   
               
               
                 60-cz 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(1,3-Dimethyl-1H-pyrazol-4-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 480.96 
                 2.99 
               
               
                   
               
               
                 60-da 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Acetylamino-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 519.94 
                 3.45 
               
               
                   
               
               
                 60-db 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Cyclohexylamino-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 512.02 
                 2.78 
               
               
                   
               
               
                 60-dc 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Ethoxy-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 458.98 
                 3.41 
               
               
                   
               
               
                 60-dd 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Pyrrolidin-1-yl-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 426.96 
                 3.53 
               
               
                   
               
               
                 60-de 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Methyl-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 476.96 
                 3.81 
               
               
                   
               
               
                 60-df 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[1,4′]Bipiperidinyl-1′-yl-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 524.01 
                 2.76 
               
               
                   
               
               
                 60-dg 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Pyridin-3-yl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 503.96 
                 2.76 
               
               
                   
               
               
                 60-dh 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Hydroxy-piperidin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 456.97 
                 2.90 
               
               
                   
               
               
                 60-di 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(2-Hydroxy-ethyl)-methyl-amino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 430.97 
                 2.85 
               
               
                   
               
               
                 60-dj 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-pyridin-2-ylamino)-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 465.93 
                 2.88 
               
               
                   
               
               
                 60-dk 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(1-Carbamoyl-piperidin-4-ylmethyl)-amino]-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 513.00 
                 2.96 
               
               
                   
               
               
                 60-dl 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Pyrrol-1-yl-ethylamino)-benzenesulfonic acid 2-cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 465.97 
                 3.43 
               
               
                   
               
               
                 60-dm 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Cyclopentyl-piperazin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 510.01 
                 2.88 
               
               
                   
               
               
                 60-dn 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Propoxy-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 458.99 
                 3.43 
               
               
                   
               
               
                 60-do 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Cyclohexylamino-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 512.03 
                 2.86 
               
               
                   
               
               
                 60-dp 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1H-Indol-5-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 487.96 
                 2.76 
               
               
                   
               
               
                 60-dq 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Amino-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 477.94 
                 2.80 
               
               
                   
               
               
                 60-dr 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2S-Methoxymethyl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 470.99 
                 3.65 
               
               
                   
               
               
                 60-ds 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[4-(2-Hydroxy-ethyl)-piperidin-1-yl]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 485.01 
                 3.21 
               
               
                   
               
               
                 60-dt 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(2-Hydroxy-ethyl)-piperidin-1-yl]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 485.02 
                 3.23 
               
               
                   
               
               
                 60-du 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Isopropylamino-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 458.01 
                 2.73 
               
               
                   
               
               
                 60-dv 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl-amino}-propionic acid 
                 444.95 
                 2.81 
               
               
                   
               
               
                 60-dw 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[Methyl-(2-methylamino-ethyl)-amino]-benzenesulfonicacid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 443.99 
                 2.46 
               
               
                   
               
               
                 60-dx 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Acetylamino-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 483.98 
                 2.88 
               
               
                   
               
               
                 60-dy 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 {4-[2-(Cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yloxy-sulfonyl]-phenylamino}-acetic acid 
                 430.94 
                 2.88 
               
               
                   
               
               
                 60-dz 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Hydroxy-piperidin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 456.98 
                 2.96 
               
               
                   
               
               
                 60-ea 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Dimethylamino-benzylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 506.02 
                 2.78 
               
               
                   
               
               
                 60-eb 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Imidazol-1-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 480.98 
                 2.78 
               
               
                   
               
               
                 60-ec 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Quinoxalin-5-ylamino)-benzenesulfonic acid 2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 500.96 
                 3.41 
               
               
                   
               
               
                 60-ed 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[4-(2-Hydroxy-ethyl)-piperazin-1-yl]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 486.00 
                 2.48 
               
               
                   
               
               
                 60-ef 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-1,1-dimethyl-ethylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 444.98 
                 2.49 
               
               
                   
               
               
                 60-eg 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperidine-4-carboxylic acid 
                 484.98 
                 3.11 
               
               
                   
               
               
                 60-eh 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 6-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenylamino}-hexanoic acid methyl ester 
                 500.99 
                 3.70 
               
               
                   
               
               
                 60-ei 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[4-(4-Methoxy-phenyl)-piperazin-1-yl]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 547.99 
                 3.23 
               
               
                   
               
               
                 60-ej 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[4-(2-Methoxy-ethyl)-piperazin-1-yl]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 500.01 
                 2.51 
               
               
                   
               
               
                 60-ek 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(2-Hydroxy-ethyl)-phenyl-amino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 492.96 
                 3.65 
               
               
                   
               
               
                 60-el 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Furan-2-ylmethyl)-amino]-benzenesulfonic acid2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 452.94 
                 3.38 
               
               
                   
               
               
                 60-em 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Carbamoyl-piperidin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 483.98 
                 2.90 
               
               
                   
               
               
                 60-en 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Methyl-piperazin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 456.00 
                 2.54 
               
               
                   
               
               
                 60-eo 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,6-Dimethyl-morpholin-4-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 471.01 
                 3.61 
               
               
                   
               
               
                 60-ep 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Phenyl-piperazin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-benzoimidazol-5-yl ester 
                 518.00 
                 3.71 
               
               
                   
               
               
                 60-eq 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Pyridin-2-yl-piperazin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 519.00 
                 2.70 
               
               
                   
               
               
                 60-er 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Diethylamino-1-methyl-butylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 514.06 
                 2.98 
               
               
                   
               
               
                 60-es 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperazine-1-carboxylicacid ethyl ester 
                 513.99 
                 3.38 
               
               
                   
               
               
                 60-et 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(5-Hydroxy-naphthalen-1-ylamino)-benzenesulfonic acid2-(cyclopropanecarbonylamino)-1H-benzoimidazol-5-yl ester 
                 514.95 
                 3.35 
               
               
                   
               
               
                 60-eu 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(4-Hydroxy-3-methoxy-phenyl)-ethylamino]-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 522.97 
                 2.88 
               
               
                   
               
               
                 60-ev 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(9H-Purin-6-ylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 490.94 
                 2.71 
               
               
                   
               
               
                 60-ew 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1-{4-[2-(Cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yloxysulfonyl]-phenyl}-piperidine-3-carboxylic acid 
                 484.98 
                 3.18 
               
               
                   
               
               
                 60-ex 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3,3-Dimethyl-piperidin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 469.03 
                 4.18 
               
               
                   
               
               
                 60-ey 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Methyl-piperidin-1-yl)-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 455.02 
                 3.83 
               
               
                   
               
               
                 60-ez 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Pyridin-2-yl-ethylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 477.98 
                 2.58 
               
               
                   
               
               
                 60-fa 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxymethyl-phenyl-amino)-benzene-sulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 478.98 
                 2.80 
               
               
                   
               
               
                 60-fb 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Oxo-2,3-dihydro-1H-pyrimidin-4-ylideneamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 466.94 
                 2.46 
               
               
                   
               
               
                 60-fc 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Piperidin-1-yl-propylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 498.05 
                 2.85 
               
               
                   
               
               
                 60-fd 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(1H-Indol-3-yl)-ethylamino]-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 515.98 
                 3.89 
               
               
                   
               
               
                 60-fe 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(5-Carbamoyl-1H-imidazol-4-ylamino)-benzenesulfonicacid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 481.96 
                 2.68 
               
               
                   
               
               
                 60-ff 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-Hydroxymethyl-butylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 459.01 
                 3.33 
               
               
                   
               
               
                 60-fg 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-Benzyl-piperidin-4-ylamino)-benzenesulfonic acid2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 546.04 
                 2.81 
               
               
                   
               
               
                 60-fh 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{4-[2-(2-Hydroxy-ethoxy)-ethyl)-ethyl]-piperazin-1-yl}-benzenesulfonic acid2-(cyclopropane-carbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 530.01 
                 2.49 
               
               
                   
               
               
                 60-fi 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Methyl-[1,4]diazepan-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 470.03 
                 2.48 
               
               
                   
               
               
                 60-fj 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Azepan-1-yl-propylamino)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 512.06 
                 2.73 
               
               
                   
               
               
                 60-fk 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,6-cis-Dimethyl-morpholin-4-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 512.04 
                 3.73 
               
               
                   
               
               
                 60-fl 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2S-Hydroxymethyl-pyrrolidin-1-yl)-benzenesulfonic acid 2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 457.00 
                 3.19 
               
               
                   
               
               
                 60-fm 
                 55 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[4-(3-Pyrrolidin-1-yl-propyl)-[1,4]diazepan-1-yl]-benzenesulfonic acid2-(cyclopropanecarbonyl-amino)-1H-benzoimidazol-5-yl ester 
                 567.07 
                 2.39 
               
               
                   
               
             
          
         
       
     
     Example 61 
     Preparation of 4-trifluoromethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     step 1: 7.82 g of 4-amino-3-nitrophenol in 460 ml of methanol were hydrogenated with catalytic amount of palladium on carbon (800 mg, 10% Pd/C). After hydrogen uptake was complete, the catalyst was filtered off, washed with methanol and the filtrate was concentred under reduced pressure to give 6 g of crude 3,4-diaminophenol. 
     Step 2: 6 g of 3,4-diaminophenol were combined with 9.8 g of 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea in 50 ml methanol and 30 ml acetic acid. The reaction mixture was refluxed for 4 hours. Solvents were then evaporated under reduce pressure yielding 10.8 g crude (5-hydroxy-1H-benzoimidazol-2-yl)-carbamic acid methyl ester. The residue was subjected to flash chromatography eluting with a mixture of dichloromethane-methanol (9:1; v/v) to give 5.6 g of a beige solid. Mass spectrum: 208 [M+H] + , retention time=0.56 minute. 
     Step 3: A stirred solution of (5-hydroxy-1H-benzoimidazol-2-yl)-carbamic acid methyl ester (100 mg) and 4-trifluoromethoxy-benzenesulfonyl chloride (126 mg) in acetone (3 ml) was treated with triethylamine (130 μl). After stirring at ambient temperature for 4 hours, the reaction mixture was evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of ethyl acetate and heptane (1:1, v/v) to give 4-trifluoromethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester (65 mg) as an off-white solid Mass spectrum: 432 [M+H] + ; retention time=15.04 minutes. 
     Example 62 
     By using a method similar to that for the preparation of example 61, combining (5-hydroxy-1H-benzoimidazol-2-yl)-carbamic acid methyl ester with suitable benzene sulfonyl chloride were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention time 
                   
               
               
                 Example 
                 Benzene sulfonyl chloride 
                 Compound 
                 (minutes) 
                 Mass [M + H] +   
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 62-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3,5-Dimethyl-isoxazole-4-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 15.87 
                 367 
               
               
                   
               
               
                 62-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 Thiophene-2-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 14.95 
                 354 
               
               
                   
               
               
                 62-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 5-Isoxazol-3-yl-thiophene-2-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 12.58 
                 421 
               
               
                   
               
               
                 62-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2-Fluoro-benzenesulfonic acid2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 10.37 
                 366 
               
               
                   
               
               
                 62-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 5-(1-Methyl-5-trifluoromethyl-1H-pyrazol-3-yl)-thiophene-2-sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 16.58 
                 502 
               
               
                   
               
               
                 62-f 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-Trifluoromethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 4.06 
                 432 
               
               
                   
               
               
                 62-g 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2-Trifluoromethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 13.06 
                 432 
               
               
                   
               
               
                 62-h 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2,6-Difluoro-benzenesulfonicacid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 9.76 
                 384 
               
               
                   
               
               
                 62-i 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-Methoxy-benzenesulfonicacid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 10.16 
                 378 
               
               
                   
               
               
                 62-j 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-(2-Methoxycarbonylamino-1H-benzoimidazol-5-yloxysulfonyl)-thiophene-2-carboxylic acid methyl ester 
                 8.07 
                 412 
               
               
                   
               
               
                 62-k 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3,4-Dimethoxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 9.32 
                 408 
               
               
                   
               
               
                 62-l 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-Nitro-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 11.56 
                 393 
               
               
                   
               
               
                 62-m 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-Trifluoromethyl-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 14.19 
                 416 
               
               
                   
               
               
                 62-n 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2-Cyano-benzenesulfonic acid2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 9.43 
                 373 
               
               
                   
               
               
                 62-o 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2-Trifluoromethyl-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 13.26 
                 416 
               
               
                   
               
               
                 62-p 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2,4-Difluoro-benzenesulfonicacid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 11.35 
                 384 
               
               
                   
               
               
                 62-q 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 5-Fluoro-2-methyl-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 12.93 
                 380 
               
               
                   
               
               
                 62-r 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-Fluoro-benzenesulfonic acid2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 11.19 
                 366 
               
               
                   
               
               
                 62-s 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyano-benzenesulfonic acid2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 10.37 
                 373 
               
               
                   
               
               
                 62-t 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2-Methoxy-5-(2-methoxycarbonylamino-3H-benzoimidazol-5-yloxysulfonyl)-thiophene-3-carboxylic acid methyl ester 
                 8.52 
                 442 
               
               
                   
               
               
                 62-u 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1,3,5-Trimethyl-1H-pyrazole-4-sulfonic acid 2-methoxycarbonylamino-3H-benzoimidazal-5-yl ester 
                 6.96 
                 380 
               
               
                   
               
               
                 62-v 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 6-Morpholin-4-yl-pyridine-3-sulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester 
                 7.82 
                 434 
               
               
                   
               
               
                 62-w 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 2,4,6-Trifluoro-benzenesulfonicacid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 3.34 
                 402 
               
               
                   
               
             
          
         
       
     
     Example 63 
     Preparation of 4-benzyloxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     Step 1: A stirred solution of 4-amino-3-nitro-phenol (3 g) and benzoic acid 4-chlorosulfonyl-phenyl ester (5.7 g) in acetone (80 ml) was treated with triethylamine (5.4 ml). After stirring at ambient temperature for 14 hours, the reaction mixture was evaporated. The residue was triturated with diisopropylic ether, filtered off and dried under vacuum to give 5.22 g of benzoic acid 4-amino-3-nitro-phenoxysulfonyl)-phenyl ester (5.22 g) as a yellow solid Mass spectrum: 401 [M+H] + ; retention time=4.59 minutes. 
     Step 2: A solution benzoic acid 4-amino-3-nitro-phenoxysulfonyl)-phenyl ester (3 g) and 2N aqueous solution of sodium hydroxyde in methanol (55 ml) was reluxed for 2 hours. The reaction mixture was concentrated and water (100 ml) and ethyl acetate (100 ml) were added. The organic layer was dried over magnesium sulfate then evaporated to give 1.77 g of crude 4-hydroxy-benzenesulfonic acid 4-amino-3-nitro-phenyl ester. 
     Step 3: A solution of cesium carbonate (156 mg) in water (0.3 ml) was added to a solution of 4-hydroxy-benzenesulfonic acid 4-amino-3-nitro-phenyl ester (150 mg) and benzyl bromide (58 μl) in dimethylformamide (3 ml). The reaction mixture was heated at 80° C. for 3 hours then allowed to cool to ambient temperature, poured into water (25 ml) and extracted three times with ethyl acetate (30 ml). The combined extracts were dried over magnesium sulfate then evaporated to give 189 mg of crude 4-benzyloxy-benzenesulfonic acid 4-amino-3-nitrophenyl ester. 
     Step 4: Sodium dithionite (624 mg) was added to a solution of 4-benzyloxy-benzenesulfonic acid 4-amino-3-nitrophenyl ester (180 mg) and sodium hydroxyde (0.5 N, 3.1 ml) in ethanol (6 ml) at 80° C. The reaction mixture was stirred at 80° C. for 10 minutes then filtered and the filtrate was evaporated. The residue was extracted three times with ethyl acetate (15 ml). The combined extracts were dried over magnesium sulfate then evaporated to 137 mg of crude 4-benzyloxy-benzenesulfonic acid 3,4-diamino-phenyl ester. 
     Step 5: preparation of 4-Benzyloxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
     To a solution of 4-benzyloxy-benzenesulfonic acid 3,4-diamino-phenyl ester (134 mg) in acetic acid (0.83 ml) and methanol (2.5 ml) at 80° C. was added 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea (89 mg). The reaction mixture was refluxed for 2 hours then allowed to cool to ambient temperature and stirred at this temperature for 14 hours. The resultant precipitate was filtered, washed with diethyl ether and dried under vacuum to afford 4-benzyloxy-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester as a beige solid. Mass spectrum: 454 [M+H] + ; retention time=11.46 minutes. 
     Example 64 
     By using a method similar to that for the preparation of example 63, combining in step 3 the 4-hydroxy-benzenesulfonic acid 4-amino-3-nitro-phenyl ester with suitable alkyl halide were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention time 
                   
               
               
                 Example 
                 Alkyl halide 
                 Compound 
                 (minutes) 
                 Mass [M + H] +   
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 64-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Ethoxy-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester 
                 12.34 
                 392 
               
               
                   
               
               
                 64-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Morpholin-4-yl-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester 
                 3.24 
                 477 
               
               
                   
               
               
                 64-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester 
                 9.97 
                 422 
               
               
                   
               
               
                 64-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-piperidin-1-yl-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester 
                 3.94 
                 475 
               
               
                   
               
               
                 64-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 [4-(2-Methoxycarbonylamino-3H-benzoimidazol-5-yloxysulfonyl)-phenoxy]-acetic acid 
                 7.23 
                 422 
               
               
                   
               
             
          
         
       
     
     Example 65 
     Preparation of 4-(2-oxo-2-pyrrolidin-1-yl-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl-ester 
     
       
                 
         
             
             
         
      
     
     A solution of [4-(2-methoxycarbonylamino-3H-benzoimidazol-5-yloxysulfonyl)-phenoxy]-acetic acid (40 mg, example 64-e) in dry dimethylformamide (3 ml) was treated with N-{(dimethylamino)(1H-1,2,3-triazolo[4,5-b]pyridin-1-yl)methylene}-N-methylmethanaminium hexafluorophosphate N-oxide (39 mg) and diisopropylethylamine (50 μl). After stirring at ambient temperature for 30 minutes, pyrrolidine (21 μl) was added and the mixture stirred at room temperature for a further 3 hours. The solvent was removed in vacuo and the residue was purified by triggered LC/MS to give 4-(2-oxo-2-pyrrolidin-1-yl-ethoxy)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl-ester as an off-white solid. Mass spectrum: 475[M+H] + ; retention time=8.39 minutes. 
     Example 66 
     By using a method similar to that for the preparation of example 65, combining [4-(2-methoxycarbonylamino-3H-benzoimidazol-5-yloxysulfonyl)-phenoxy]-acetic acid with suitable amine were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention time 
                   
               
               
                 Example 
                 Amine 
                 Compound 
                 (minutes) 
                 Mass [M + H]+ 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 66-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[2-(4-Methyl-piperazin-1-yl)-2-oxo-ethoxy]-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 1.96 
                 504 
               
               
                   
               
               
                 66-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(3-diethylamino-propylcarbamoyl)-methoxy]-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 1.96 
                 534 
               
               
                   
               
               
                 66-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-{[(furan-2-ylmethyl)-carbamoyl]-methoxy}-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 10.89 
                 501 
               
               
                   
               
             
          
         
       
     
     Example 67 
     Preparation of 4-(cyclopropylmethyl-amino)-benzene sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     Step 1: preparation of 4-(cyclopropylmethyl-amino)-benzenesulfonic acid 4-amino-3-nitro-phenyl ester 
     A solution of 4-fluoro-benzenesulfonic acid 4-amino-3-nitro-phenyl ester (800 mg) and cyclopropylmethylamine (890 μl) in N-methylpyrrolidinone (8 ml) was heated at 110° C. in a sealed tube for 14 hours. The reaction mixture was then poured into water (150 ml) and extracted three times with ethyl acetate (40 ml). The combined extracts were dried over magnesium sulfate and then evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of ethyl acetate and heptane (50:50, v/v) to give 4-(cyclopropylmethyl-amino)-benzenesulfonic acid 4-amino-3-nitro-phenyl ester (786 mg) as a yellow solid. 
     Step 2: Sodium dithionite (3 g) was added to a solution of 4-(cyclopropylmethyl-amino)-benzenesulfonic acid 4-amino-3-nitro-phenyl ester (783 mg) and sodium hydroxyde (0.5 N, 15 ml) in ethanol (30 ml) at 80° C. The reaction mixture was stirred at 80° C. for 10 minutes then filtered then the filtrate was evaporated. The residue was extracted three times with ethyl acetate (30 ml). The combined extracts were dried over magnesium sulfate then evaporated to give 652 mg of 4-(cyclopropylmethyl-amino)-benzenesulfonic acid 3,4-diamino-phenyl ester. 
     Step 3: To a solution of 4-(cyclopropylmethyl-amino)-benzenesulfonic acid 3,4-diamino-phenyl ester (648 mg) in acetic acid (4.5 ml) and methanol (40 ml) at 80° C. was added 1,3-bis(methoxycarbonyl)-2-methyl-2-thiopseudourea (580 mg). The reaction mixture was refluxed for 4 hours then allowed to cool to ambient temperature and stirred at this temperature for 14 hours. The resultant precipitate was filtered, washed with diethyl ether and dried under vacuum to afford 4-(cyclopropylmethyl-amino)-benzene sulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester (378 mg) as a beige solid. Mass spectrum: 417 [M+H] + ; retention time=13.16 minutes. 
     Example 68 
     By using a method similar to that for the preparation of example 67, combining 4-fluoro-benzenesulfonic acid 4-amino-3-nitro-phenyl ester with suitable amine in step 1 were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention time 
                 Mass 
               
               
                 Example 
                 Amine 
                 Compound 
                 (minutes) 
                 [M + H]+ 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 68-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-methoxy-ethylamino)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 8.89 
                 421 
               
               
                   
               
               
                 68-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-hydroxy-1-methyl-ethylamino)-benzenesulfonicacid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 6.84 
                 421 
               
               
                   
               
               
                 68-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(benzylamino)-benzenesulfonic acid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 4.4 
                 453 
               
               
                   
               
               
                 68-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonicacid 2-methoxycarbonylamino-1H-benzoimidazol-5-yl ester 
                 2.44 
                 476 
               
               
                   
               
               
                 68-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Piperidin-4-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 2.77 
                 460 
               
               
                   
               
               
                 68-f 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(1-Ethyl-pyrrolidin-2-ylmethyl)-amino]-benzenesulfonicacid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 2.3 
                 474 
               
               
                   
               
             
          
         
       
     
     Example 69 
     Preparation of 4-cyclopentylamino-benzenesulfonic acid 2-(3,4-dimethoxy-phenylamino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     Step 1: A solution of 1-benzyl-6-methoxy-1H-benzoimidazole (3 g) in dry tetrahydrofuran (65 ml), cooled to −78° C., was treated with a solution of n-butyllithium in hexanes (12 ml, 15%). After stirring for 45 minutes the mixture was treated with N-chlorosuccinimide (2.24 g in 65 ml of tetrahydrofuran) then allowed to warm slowly to ambient temperature. The reaction mixture was allowed to stir at ambient temperature for 2 hours then treated with a saturated aqueous solution of ammonium chloride (100 ml) and extracted three times with ethyl acetate (65 ml). The combined extracts were dried over magnesium sulfate and then evaporated. The residue was subjected to flash column chromatography on silica eluting with a mixture of ethyl acetate and hexane (1:1, v/v) to 1-benzyl-2-chloro-6-methoxy-1H-benzoimidazole (2.09 g) as a yellow solid. Mass spectrum: 273 [M+H] + , retention time=3.93 minutes. 
     Step 2: A mixture of 1-benzyl-2-chloro-6-methoxy-1H-benzoimidazole (600 mg), hydrobromic acid (48%, 11 ml) and glacial acetic acid (6 ml) was heated under reflux for 1 hour. After cooling the mixture was neutralised by addition of 10% sodium bicarbonate solution then extracted 3 times with dichloromethane (30 ml). The combined extracts were dried over magnesium sulfate and then evaporated to give 3-benzyl-2-chloro-3H-benzoimidazol-5-ol (470 mg) as a yellow solid. Mass spectrum: 259 [M+H] +  retention time=3.4 minutes. 
     Step 3: A mixture of 3-benzyl-2-chloro-3H-benzoimidazol-5-ol (250 mg) and 4-amino veratrole (296 mg) in N-methylpyrrolidinone (3 ml) was heated at 150° C. in a sealed tube for 4 hours then allowed to cool. The reaction mixture was then poured into water (30 ml) and extracted three times with ethyl acetate (30 ml). The combined extracts were dried over magnesium sulfate and then evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of dichloromethane and methanol (95:5, v/v) to give 3-benzyl-2-(3,4-dimethoxy-phenylamino)-3H-benzoimidazol-5-ol (141 mg) as a yellow solid. Mass spectrum: 376 [M+H] +  retention time: 3.44 minutes. 
     Step 4: A stirred solution of 3-benzyl-2-(3,4-dimethoxy-phenylamino)-3H-benzoimidazol-5-ol (141 mg) and 4-fluoro-benzenesulfonyl chloride (190 mg) in acetone (8 ml) was treated with triethylamine (258 μl). After stirring at ambient temperature for 4 hours, the reaction mixture was evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of ethyl acetate and heptane (1:1, v/v) to give 4-fluoro-benzenesulfonic acid 3-benzyl-2-(3,4-dimethoxy-phenylamino)-3H-benzoimidazol-5-yl ester (157 mg) as a yellow solid. Mass spectrum: 534 [M+H] + , retention time: 3.7 minutes. 
     Step 5: A solution of 4-fluoro-benzenesulfonic acid 3-benzyl-2-(3,4-dimethoxy-phenylamino)-3H-benzoimidazol-5-yl ester (151 mg) and cyclopentylamine (118 μl) in N-methylpyrrolidinone (1.5 ml) was heated at 110° C. in a sealed tube for 3 hours. The reaction mixture was allowed to cool then poured into water (30 ml) and extracted three times with ethyl acetate. The combined extracts were dried over magnesium sulfate then evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of ethyl acetate and heptane (1:1, v/v) to give 4-cyclopentylamino-benzenesulfonic acid 3-benzyl-2-(3,4-dimethoxy-phenylamino)-3H-benzoimidazol-5-yl ester (122 mg) as a brown solid. Mass spectrum: 599 [M+H] + , retention time=4.0 minutes. 
     Example 70 
     By using a method similar to that for the preparation of example 69, combining 3-benzyl-2-chloro-3H-benzoimidazol-5-ol with suitable amine in step 3 were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention time 
                 Mass 
               
               
                 Example 
                 Amine 
                 Compound 
                 (minute) 
                 [M + H] +   
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 70-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-phenylamino-1H-benzoimidazol-5-yl ester 
                 12.31 
                 449 
               
               
                   
               
               
                 70-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(4-morpholin-4-yl-phenylamino)-1H-benzoimidazol-5-yl ester 
                 11.58 
                 534 
               
               
                   
               
               
                 70-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3,5-dimethyl-phenylamino)-1H-benzoimidazol-5-yl ester 
                 9.55 
                 477 
               
               
                   
               
               
                 70-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(4-methoxy-phenylamino)-1H-benzoimidazol-5-yl ester 
                 8.69 
                 479 
               
               
                   
               
               
                 70-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(4-dimethylamino-phenylamino)-1H-benzoimidazol-5-yl ester 
                 8.59 
                 492 
               
               
                   
               
               
                 70-f 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-(3-methoxy-5-trifluoromethyl-phenylamino)-1H-benzoimidazol-5-yl ester 
                 11.94 
                 547 
               
               
                   
               
               
                 70-g 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-[5-(4-Cyclopentylamino-benzenesulfonyloxy)-1H-benzoimidazol-2-ylamino]-benzoic acid ethyl ester 
                 10.13 
                 521 
               
               
                   
               
               
                 70-h 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Cyclopentylamino-benzenesulfonic acid 2-[(4-(4-methyl-piperazin-1-yl)-phenylamino)-1H-benzoimidazol-5-yl ester 
                 6.64 
                 547 
               
               
                   
               
             
          
         
       
     
     Example 71 
     Preparation of 4-cyclopentylamino-benzenesulfonic acid 2-(3-phenyl-propionylamino)-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     A solution of 3-phenyl propionic acid (9.7 mg) in dry dimethylformamide (0.6 ml) was treated with N-{(dimethylamino)(1H-1,2,3-triazolo[4,5-b]pyridin-1-yl)methylene}-N-methylmethanaminium hexafluorophosphate N-oxide (21 mg) and diisopropylethylamine (12 μl). After stirring at ambient temperature for 30 minutes, 4-cyclopentylamino-benzenesulfonic acid 2-amino-3H-benzoimidazol-5-yl ester (20 mg) was added and the mixture stirred at room temperature for a further 3 hours. The solvent was removed under vacuo and the residue was purified by triggered LC/MS to give 4-cyclopentylamino-benzenesulfonic acid 2-(3-phenyl-propionylamino)-1H-benzoimidazol-5-yl ester as an off-white solid (11 mg). Mass spectrum: 505 [M+H] + ; retention time=4.59 minutes. 
     Example 72 
     Preparation of 4-cyclopentylamino-benzenesulfonic acid 2-[2-2-methoxy-ethoxy)-acetylamino]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     By proceeding in a manner similar to example 71 above but using (2-methoxy-ethoxy)-acetic acid there was prepared 4-cyclopentylamino-benzenesulfonic acid 2-[2-2-methoxy-ethoxy)-acetylamino]-1H-benzoimidazol-5-yl ester as an off-white solid. Mass spectrum: 489 [M+H] + ; retention time=4.06 minutes. 
     Example 73 
     Preparation of 4-fluoro-benzenesulfonic acid 2-(3(chloro-4-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     Step 1: A stirred solution of 4-fluoro-benzenesulfonic acid 2-tert-butoxycarbonylamino-3H-benzoimidazol-5-yl ester (Example 55 (step 3), 200 mg) in dry dimethylformamide (3 ml) was treated with sodium hydride (12 mg, 60% dispersion in mineral oil). After stirring for 30 minutes the mixture was treated with a solution of 3-chloro-4-methoxy-benzyl bromide (94 mg) in dimethylformamide (1 ml) and stirring was continued for a further 3 hours. The reaction mixture was poured into water (10 ml) and then extracted three times with ethyl acetate (10 ml). The combined extracts were dried over magnesium sulfate and then evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of ethyl acetate and heptane (1:2, v/v) to give 4-fluoro-benzenesulfonic acid 2-[tert-butoxycarbonyl-(3-chloro-4-methoxy-benzyl)-amino]-3H-benzoimidazol-5-yl ester (70 mg) as a beige solid. 
     Step 2: preparation of 4-fluoro-benzenesulfonic acid 2-(3-(chloro-4-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester 
     Trifluoroacetic acid (1 ml) was added to a solution of 4-fluoro-benzenesulfonic acid 2-[tert-butoxycarbonyl-(3-chloro-4-methoxy-benzyl)-amino]-3H-benzoimidazol-5-yl ester (67 mg) in dichloromethane (4 ml). After cooling, the mixture was neutralised by addition of saturated sodium bicarbonate solution. Water (10 ml) was added and the solution extracted three times with dichloromethane (10 ml). The combined extracts were dried over magnesium sulfate and then evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of ethyl acetate and heptane (1:1, v/v) to give 4-fluoro-benzenesulfonic acid 2-(3-(chloro-4-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester (53 mg) as an off-white solid. Mass spectrum: 462 [M+H] + ; retention time=7.69 minutes. 
     Example 74 
     By using a method similar to that for the preparation of example 73, combining 4-fluoro-benzenesulfonic acid 2-tert-butoxycarbonylamino-3H-benzoimidazol-5-yl ester with suitable benzyl halide were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention time 
                 Mass 
               
               
                 Example 
                 Benzyl halide 
                 Compound 
                 (minute) 
                 [M + H] +   
               
               
                   
               
             
             
               
                 74-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Fluoro-benzenesulfonicacid 2-[(3-phenyl-[1,2,4]-oxadiazol-5-ylmethyl)-amino]-3H-benzoimidazol-5-yl ester 
                 8.13 
                 466 
               
               
                   
               
               
                 74-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Fluoro-benzenesulfonicacid 2-(3-chloro-benzylamino)-3H-benzoimidazol-5-yl ester 
                 7.72 
                 432 
               
               
                   
               
               
                 74-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Fluoro-benzenesulfonicacid 2-(3-methoxy-benzylamino)-3H-benzoimidazol-5-yl ester 
                 7.31 
                 428 
               
               
                   
               
               
                 74-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Fluoro-benzenesulfonicacid 2-benzylamino-3H-benzoimidazol-5-yl ester 
                 7.43 
                 398 
               
               
                   
               
             
          
         
       
     
     Example 75 
     Preparation of 4-cyclopentylamino-benzenesulfonic acid 2-benzylamino-3H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     A solution of 4-fluoro-benzenesulfonic acid 2-benzylamino-3H-benzoimidazol-5-yl ester (20 mg) and cyclopentylamine (21 μl) in N-methylpyrrolidinone (0.5 ml) was heated at 110° C. in a sealed tube for 2 hours. The reaction mixture was then purified by triggered LC/MS to give 4-cyclopentylamino-benzenesulfonic acid 2-benzylamino-3H-benzoimidazol-5-yl ester as an off-white solid (4 mg). Mass spectrum: 463 [M+H] + ; retention time=8.35 minutes. 
     Example 76 
     By using a method similar to that for the preparation of example 75, combining cyclopentylamine with suitable 4-fluoro-benzenesulfonic acid 2-benzylamino-3H-benzoimidazol-5-yl ester (example 73, 74a–74c) were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention 
                   
               
               
                   
                   
                   
                 time 
                 Mass 
               
               
                 Example 
                 Precursor 
                 Compound 
                 (minute) 
                 [M + H] +   
               
               
                   
               
             
             
               
                 76-a 
                 74-a 
                 4-Cyclopentylamino- 
                 3.91 
                 531 
               
               
                   
                   
                 benzenesulfonic acid 2- 
               
               
                   
                   
                 [(3-phenyl- 
               
               
                   
                   
                 [1,2,4]oxadiazol-5- 
               
               
                   
                   
                 ylmethyl)-amino]-3H- 
               
               
                   
                   
                 benzoimidazol-5-yl 
               
               
                   
                   
                 ester 
               
               
                 76-b 
                 74-c 
                 4-Cyclopentylamino- 
                 8.41 
                 493 
               
               
                   
                   
                 benzenesulfonic acid 2- 
               
               
                   
                   
                 (3-methoxy- 
               
               
                   
                   
                 benzylamino)-3H- 
               
               
                   
                   
                 benzoimidazol-5-yl 
               
               
                   
                   
                 ester 
               
               
                 76-c 
                 73 
                 4-Cyclopentylamino- 
                 3.58 
                 527 
               
               
                   
                   
                 benzenesulfonic acid 2- 
               
               
                   
                   
                 (3-chloro-4-methoxy- 
               
               
                   
                   
                 benzylamino)-3H- 
               
               
                   
                   
                 benzoimidazol-5-yl 
               
               
                   
                   
                 ester 
               
               
                   
               
             
          
         
       
     
     Example 77 
     Preparation of 4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     A solution of 4-(cyclopropylmethyl-amino)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester (example 67, 40 mg) and 2-(aminoethyl)-morpholine (125 mg) in tetrahydrofuran (2 ml) and N-methylpyrrolidinone (0.2 ml) was heated at 90° C. for 36 hours. The reaction mixture was then evaporated and purified by triggered LC/MS to give 4-(cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester as an off-white solid (27 mg). Mass spectrum: 515 [M+H] + ; retention time=5.97 minutes. 
     Example 78 
     By using a method similar to that for the preparation of example 77, combining 4-(substituted-amino)-benzenesulfonic acid 2-methoxycarbonylamino-3H-benzoimidazol-5-yl ester [example 63, 67, 68] with suitable amine were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                   
                 Retention time 
                 Mass 
               
               
                 Example 
                 Precursor 
                 Amine 
                 Compound 
                 (minutes) 
                 [M + H] +   
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 78-a 
                 67 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 5.97 
                 515 
               
               
                   
               
               
                 78-b 
                 67 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 8.55 
                 493 
               
               
                   
               
               
                 78-c 
                 67 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 10.66 
                 460 
               
               
                   
               
               
                 78-d 
                 67 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(Cyclopropylmethyl-amino)-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 11.07 
                 430 
               
               
                   
               
               
                 78-e 
                 68-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 5.74 
                 519 
               
               
                   
               
               
                 78-f 
                 68-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 6.62 
                 497 
               
               
                   
               
               
                 78-g 
                 68-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 7.39 
                 464 
               
               
                   
               
               
                 78-h 
                 68-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Methoxy-ethylamino)-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 7.53 
                 434 
               
               
                   
               
               
                 78-i 
                 68-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonicacid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 5.36 
                 519 
               
               
                   
               
               
                 78-j 
                 68-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 6.11 
                 497 
               
               
                   
               
               
                 78-k 
                 68-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonicacid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 6.81 
                 464 
               
               
                   
               
               
                 78-l 
                 68-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-1-methyl-ethylamino)-benzenesulfonicacid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 6.94 
                 434 
               
               
                   
               
               
                 78-m 
                 63 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzyloxy-benzenesulfonicacid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 7.55 
                 552 
               
               
                   
               
               
                 78-n 
                 63 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzyloxy-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 8.99 
                 530 
               
               
                   
               
               
                 78-o 
                 63 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzyloxy-benzenesulfonicacid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 13.97 
                 497 
               
               
                   
               
               
                 78-p 
                 63 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-benzyloxy-benzenesulfonicacid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 10.12 
                 467 
               
               
                   
               
               
                 78-r 
                 68-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 4.61 
                 552 
               
               
                   
               
               
                 78-s 
                 68-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Morpholin-4-yl-ethylamino)-benzenesulfonicacid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 1.47 
                 519 
               
               
                   
               
               
                 78-t 
                 68-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Piperidin-4-ylmethyl)-amino)-benzenesulfonic acid2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 4.47 
                 558 
               
               
                   
               
               
                 78-u 
                 68-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Piperidin-4-ylmethyl)-amino]-benzenesulfonic acid2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 5.12 
                 536 
               
               
                   
               
               
                 78-v 
                 68-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Piperidin-4-ylmethyl)-amino]-benzenesulfonic acid2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 1.47 
                 503 
               
               
                   
               
               
                 78-w 
                 68-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzylamino-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 7.16 
                 551 
               
               
                   
               
               
                 78-x 
                 68-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzylamino-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 8.52 
                 529 
               
               
                   
               
               
                 78-y 
                 68-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzylamino-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 9.29 
                 496 
               
               
                   
               
               
                 78-z 
                 68-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-Benzylamino-benzenesulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 9.35 
                 466 
               
               
                   
               
               
                  78-aa 
                 68-f 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(1-ethyl-pyrrolidin-2ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl)-ureido]-1H-benzoimidazol-5-yl ester 
                 2.14 
                 549 
               
               
                   
               
             
          
         
       
     
     Example 79 
     Preparation of 4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[(4-hydroxy-piperidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     A solution of 4-fluoro-benzenesulfonic acid 2-tert-butoxycarbonylamino-3H-benzoimidazol-5-yl ester (200 mg, example 55 (Step 3) and 4-hydroxypiperidine (554 mg) in N-methylpyrrolidinone (6 ml) was heated at 110° C. for 24 hours. The reaction mixture was then poured into water (120 ml) and extracted three times with ethyl acetate (50 ml). The combined extracts were dried over magnesium sulfate and then evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of dichloromethane and methanol (95 C: 5, v/v) to give (4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[(4-hydroxy-piperidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester (125 mg) as a beige solid. Mass Spectrum: 516 [M+H] + , retention time=6.51 minutes. 
     Example 80 
     By using a method similar to that for the preparation of example 79, combining 4-fluoro-benzenesulfonic acid 2-tert-butoxycarbonylamino-3H-benzoimidazol-5-yl ester with suitable amine were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                 Retention time 
                 Mass 
               
               
                 Example 
                 Amine 
                 Compound 
                 (minutes) 
                 [M + H]+ 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 80-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-Methyl-piperazin-1-yl)-benzenesulfonic acid 2-[(4-methyl-piperazin-1-carbonyl)-amino]-3H-benzoimidazol-5-yl ester 
                 0.53 
                 514 
               
               
                   
               
               
                 80-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(tetrahydro-pyran-4-ylmethyl)-amino]-benezenesulfonic acid 2-[3-(tetrahydro-pyran-4-ylmethyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 8.14 
                 544 
               
               
                   
               
               
                 80-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Fluoro-ethylamino)-benzenesulfonic acid 2-[3-(2-fluoro-ethyl)-ureido]-3H-benzoimidazol-5-ylester 
                 7.82 
                 440 
               
               
                   
               
               
                 80-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-piperidin-1-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-piperidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 0.5 
                 440 
               
               
                   
               
               
                 80-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-phenethylamino-benzenesulfonicacid 2-(3-phenethyl-ureido)-3H-benzoimidazol-5-yl ester 
                 4.4 
                 556 
               
               
                   
               
               
                 80-f 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[3-(2-oxo-pyrrolidin-1-yl)-propylamino]-benzenesulfonic acid 2-{3-[3-(2-owo-pyrrolidin-1-yl)-propyl]-ureido}-3H-benzoimidazol-5-yl ester 
                 3.09 
                 598 
               
               
                   
               
               
                 80-g 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-fluoro-benzylamino)-benzenesulfonic acid 2-[3-(4-fluoro-benzyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 4.25 
                 564 
               
               
                   
               
               
                 80-h 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-[3-(2-hydroxy-3-methyl-propyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 7.02 
                 492 
               
               
                   
               
               
                 80-i 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-hydroxy-propylamino)-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzoimidazol-5-ylester 
                 2.7 
                 464 
               
               
                   
               
               
                 80-j 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,2,6,6-tetramethyl-piperidin-4-ylamino)-benzenesulfonic acid 2-[3-(2,2,6,6-tetramethyl-piperidin-4-yl)-ureido]-3H-benzoimidazol-5-yl ester 
                 2.44 
                 626 
               
               
                   
               
               
                 80-k 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-dimethylamino-ethylamino)-benzene sulfonic acid 2-[3-(2-dimethylamino-ethyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 0.70 
                 490 
               
               
                   
               
               
                 80-l 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-morpholin-4-yl-benzenesulfonic acid2-[(morpholine-4-carbonyl)-amino]-3H-benzoimidazol-5-yl ester 
                 3.16 
                 488 
               
               
                   
               
               
                 80-m 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-3-methoxy-propylamino)-benzenesulfonic acid 2-[3-(2-hydroxy-3-methoxy-propyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 4.71 
                 524 
               
               
                   
               
               
                 80-n 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-2-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzoimidazol-5-yl ester 
                 5.53 
                 530 
               
               
                   
               
               
                 80-o 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-hydroxy-propyl)-ureido]-3H-benzoimidazol-5-ylester 
                 2.7 
                 463 
               
               
                   
               
               
                 80-p 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(4-methoxy-benzyl)-ureido]-3H-benzoimidazol-5-ylester 
                 4.43 
                 588 
               
               
                   
               
               
                 80-q 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonic acid 2-[3-(2-pyrrolidin-1-yl-ethyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 2.19 
                 542 
               
               
                   
               
               
                 80-r 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-phenyl-ethylamino)-benzenesulfonic acid 2-[3-(1-phenyl-ethyl)-ureido]-3H-benzoimidazol-5-ylester 
                 4.63 
                 556 
               
               
                   
               
               
                 80-s 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-diethylamino-ethylamino)-benzene sulfonic acid 2-[3-(2-diethylamino-ethyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 2.75 
                 546 
               
               
                   
               
               
                 80-t 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-hydroxymethyl-cyclopentylamino)-benzenesulfonicacid 2-[3-(1-hydroxymethyl-cyclopentyl)-ureido]-3H-benzoimidazol-5-yl ester 
                 3.45 
                 544 
               
               
                   
               
               
                 80-u 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 3-(4-{2-[3-(3-Methoxycarbonyl-ethyl)-ureido]-1H-benzoimidazol-5-yloxysulfonyl}-phenylamino)-propionic acid methyl ester 
                 3.26 
                 520 
               
               
                   
               
             
          
         
       
     
     Example 81 
     Preparation of 4-(4-Hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     A solution of 4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[(4-hydroxy-piperidine-1-carbonyl)-amino]-1H-benzoimidazol-5-yl ester (example 79, 20 mg) and 2-(aminomethyl)-morpholine (50 mg) in tetrahydrofuran (1 ml) and N-methylpyrrolidinone (0.2 ml) was heated at 95° C. for 22 hours. The reaction mixture was then evaporated and purified by triggered LC/MS to give 4-(4-hydroxy-piperidin-1-yl)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-1H-benzoimidazol-5-yl ester as an off-white solid (7 mg). Mass spectrum: 545 [M+H] + ; retention time=5.47 minutes. 
     Example 82 
     By using a method similar to that for the preparation of example 81, combining [example 80a–u] with suitable amine was obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                   
                   
                   
                   
                 Retention time 
                 Mass 
               
               
                 Example 
                 Precursor 
                 Amine 
                 Compound 
                 (minutes) 
                 [M + H]+ 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 82-a 
                 80-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methyl-piperazin-1-yl)-benzene sulfonic acid2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 5.12 
                 544 
               
               
                   
               
               
                 82-b 
                 80-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methyl-piperazin-1-yl)-benzene sulfonic acid2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 4.46 
                 522 
               
               
                   
               
               
                 82-c 
                 80-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methyl-piperazin-1-yl)-benzene sulfonic acid2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 5.86 
                 489 
               
               
                   
               
               
                 82-d 
                 79 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-hydroxy-piperidin-1-yl)-benzene sulfonic acid2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 5.47 
                 545 
               
               
                   
               
               
                 82-e 
                 79 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-hydroxy-piperidin-1-yl)-benzene sulfonic acid2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 6.4 
                 523 
               
               
                   
               
               
                 82-f 
                 79 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-hydroxy-piperidin-1-yl)-benzene sulfonic acid2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 7.06 
                 490 
               
               
                   
               
               
                 82-g 
                 79 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-hydroxy-piperidin-1-yl)-benzene sulfonic acid2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
                 3.3 
                 460 
               
               
                   
               
               
                 82-h 
                 80-n 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-2-ylmethyl)-amino]-benzene sulfonicacid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 4.83 
                 552 
               
               
                   
               
               
                 82-i 
                 80-n 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-2-ylmethyl)-amino]-benzene sulfonicacid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 5.53 
                 530 
               
               
                   
               
               
                 82-j 
                 80-n 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-2-ylmethyl)-amino]-benzene sulfonicacid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 6.6 
                 497 
               
               
                   
               
               
                 82-k 
                 80-n 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Pyridin-2-ylmethyl)-amino]-benzene sulfonicacid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 6.2 
                 467 
               
               
                   
               
               
                 82-l 
                 80-i  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 6.2 
                 497 
               
               
                   
               
               
                 82-m 
                 80-i  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 5.48 
                 519 
               
               
                   
               
               
                 82-n 
                 80-i  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 6.84 
                 464 
               
               
                   
               
               
                 82-o 
                 80-i  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(3-Hydroxy-propylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
               
               
                   
               
               
                 82-p 
                 80-j  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,2,6,6-tetramethyl-piperidin-4-ylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 0.41 
                 600 
               
               
                   
               
               
                 82-q 
                 80-j  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2,2,6,6-tetramethyl-pipendin-4-ylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 0.42 
                 576 
               
               
                   
               
               
                 82-r 
                 80-k 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-dimethylamino-ethylamino)-benzenesulfonicacid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 0.41 
                 532 
               
               
                   
               
               
                 82-s 
                 80-k 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-dimethylamino-ethylamino)-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-ylester 
                 0.41 
                 510 
               
               
                   
               
               
                 82-t 
                 80-l  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-morpholin-4-yl-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 0.4 
                 531 
               
               
                   
               
               
                 82-u 
                 80-l  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-morpholin-4-yl-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 2.9 
                 509 
               
               
                   
               
               
                 82-v 
                 80-f  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[3-(2-oxo-pyrrolidin-1-yl)-propylamino]-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 2.6 
                 586 
               
               
                   
               
               
                 82-w 
                 80-f  
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[3-(2-oxo-pyrrolidin-1-yl)-propylamino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 2.46 
                 485 
               
               
                   
               
               
                 82-x 
                 80-g 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-fluoro-benzylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
                 9.29 
                 514 
               
               
                   
               
               
                 82-y 
                 80-h 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 6.61 
                 511 
               
               
                   
               
               
                 82-z 
                 80-h 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 6.29 
                 533 
               
               
                   
               
               
                  82-aa 
                 80-h 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 2.89 
                 478 
               
               
                   
               
               
                  82-ab 
                 80-h 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Hydroxy-2-methyl-propylamino)-benzenesulfonic acid-2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester 
                 7.33 
                 478 
               
               
                   
               
               
                  82-ac 
                 80-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 7.28 
                 537 
               
               
                   
               
               
                  82-ad 
                 80-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 6.88 
                 559 
               
               
                   
               
               
                  82-ae 
                 80-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 8.67 
                 504 
               
               
                   
               
               
                  82-af 
                 80-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-[(Tetrahydro-pyran-4-ylmethyl)-amino]-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester 
                 8.03 
                 504 
               
               
                   
               
               
                  82-ag 
                 80-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-fluoro-ethylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 2.5 
                 507 
               
               
                   
               
               
                  82-ah 
                 80-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-fluoro-ethylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 2.46 
                 485 
               
               
                   
               
               
                  82-ai 
                 80-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Piperidin-1-yl-ethylamino)-benzenesulfonicacid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 5.47 
                 550 
               
               
                   
               
               
                  82-aj 
                 80-d 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-Piperidin-1-yl-ethylamino)-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 4.25 
                 572 
               
               
                   
               
               
                 82-ak 
                 80-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-phenethylamino-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 3.14 
                 565 
               
               
                   
               
               
                  82-al 
                 80-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-phenethylamino-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 3.78 
                 510 
               
               
                   
               
               
                  82-am 
                 80-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-phenethylamino-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
                 3.83 
                 480 
               
               
                   
               
               
                  82-an 
                 80-e 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-phenethylamino-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester 
                 3.57 
                 510 
               
               
                   
               
               
                  82-ao 
                 80-o 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-hydroxy-propylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 2.36 
                 519 
               
               
                   
               
               
                  82-ap 
                 80-o 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-hydroxy-propylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
                 2.91 
                 434 
               
               
                   
               
               
                  82-aq 
                 80-p 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(2-morpholin-4-yl-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 3.03 
                 581 
               
               
                   
               
               
                  82-ar 
                 80-p 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
                 3.67 
                 496 
               
               
                   
               
               
                  82-as 
                 80-p 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-yl ester 
                 3.41 
                 559 
               
               
                   
               
               
                  82-at 
                 80-p 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(3-hydroxy-propyl)-ureido]-3H-benzimidazol-5-yl ester 
                 3.41 
                 526 
               
               
                   
               
               
                  82-au 
                 80-p 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(4-methoxy-benzylamino)-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-yl ester 
                 3.61 
                 526 
               
               
                   
               
               
                  82-av 
                 80-q 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonicacid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
                 2.26 
                 473 
               
               
                   
               
               
                  82-aw 
                 80-q 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-ylester 
                 2.08 
                 536 
               
               
                   
               
               
                  82-ax 
                 80-q 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-pyrrolidin-1-yl-ethylamino)-benzenesulfonicacid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzimidazol-5-ylester 
                 2.25 
                 503 
               
               
                   
               
               
                  82-ay 
                 80-r 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(1-phenyl-ethylamino)-benzenesulfonic acid 2-(3-ethyl-ureido)-3H-benzimidazol-5-yl ester 
                 3.76 
                 480 
               
               
                   
               
               
                  82-az 
                 80-s 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 4-(2-diethylamino-ethylamino)-benzenesulfonicacid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzimidazol-5-ylester 
                 3.5 
                 543 
               
               
                   
               
             
          
         
       
     
     Example 83 
     Preparation of 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(2-methoxy-ethyl)-urea 
     
       
                 
         
             
             
         
      
     
     A solution of (6-hydroxy-1H-benzoimidazol-2-yl)-carbamic acid methyl ester (300 mg, example 61) and 2-methoxy-ethylamine (630 IA) in N-methylpyrrolidinone (8 ml) was heated at 90° C. in a sealed tube for 20 hours. The reaction mixture was poured into water (160 ml) and extracted three times with ethyl acetate (40 ml). The combined extracts were dried over magnesium sulfate and then evaporated. The residue was subjected to flash chromatography on silica eluting with a mixture of dichloromethane and methanol (95 C:5 C, v/v) to 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(2-methoxy-ethyl)-urea as a yellow solid (180 mg). Mass spectrum: 251 [M+H] + ; retention time=0.55 minutes. 
     Example 84(a) 
     Preparation of 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-pyridin-2-ylmethyl-urea 
     
       
                 
         
             
             
         
      
     
     By proceeding in a manner similar to example 83 above but using 2-(aminomethyl)-pyridine there was prepared 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3pyridin-2-ylmethyl-urea as a beige solid. Mass spectrum: 284 [M+H] + ; retention time=0.55 minutes. 
     Example 84(b) 
     Preparation of 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(2-morpholin-4-yl-ethyl)-urea 
     
       
                 
         
             
             
         
      
     
     By proceeding in a manner similar to example 83 above but using 2-(aminoethyl)-morpholine there was prepared 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(2-morpholin-4-yl-ethyl)-urea as a beige solid. Mass spectrum: 306 [M+H] + ; retention time=1.02 minute. 
     Example 84(c) 
     Preparation of 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(ethyl)-urea 
     
       
                 
         
             
             
         
      
     
     By proceeding in a manner similar to example 83 above but using ethylamine there was prepared 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(ethyl)-urea as a beige solid. Mass spectrum: 367 [M+H] + ; retention time=1.36 minute. 
     Example 85 
     Preparation of thiophene-2-sulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     A stirred solution of 1-ethyl-3-(6-hydroxy-1H-benzoimidazol-2-yl)-urea (35 mg, example 84-c) and thiophene-2-sulfonyl chloride (18 mg) in acetone (3 ml) was treated with triethylamine (25 μl). After stirring at ambient temperature for 4 hours, the reaction mixture was evaporated. The residue was filtered and the filtrate evaporated. The residue was directly purified by LCMS triggered purification to give thiophene-2-sulfonic acid 2-[3-(2-ethyl)-ureido]-1H-benzoimidazol-5-yl ester (14 mg) as a off-white solid Mass spectrum: 367 [M+H] + ; retention time=7.88 minutes. 
     Example 86 
     By using a method similar to that for the preparation of example 85, combining thiophene-2-sulfonyl chloride with suitable 1-(6-hydroxy-1H-benzoimidazol-2-yl)-urea (example 83, 84) were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
               
               
                 Example 
                 1-(6-hydroxy-1H-benzoimidazol-2-yl)-urea 
                 Compound 
                 Retention time (minutes) 
                 Masse [M + H]+ 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 86-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 Thiophene-2-sulfonicacid 2-[3-(2-methoxy-eth-yl)-ureido]-1H-benzo-imidazol-5-yl ester 
                 2.99 
                 397 
               
               
                   
               
               
                 86-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 Thiophene-2-sulfonicacid 2-[3-(2-morpholin-4-yl-eth-yl)-ureido]-1H-benzo-imidazol-5-yl ester 
                 2.8 
                 452 
               
               
                   
               
               
                 86-c 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 Thiophene-2-sulfonicacid 2-(3-pyridin-2-ylmeth-yl)-ureido]-1H-benzo-imidazol-5-yl ester 
                 6.52 
                 430 
               
               
                   
               
             
          
         
       
     
     Example 87 
     Preparation of benzoic acid 4-{2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yloxysulfonyl}-phenyl ester 
     
       
                 
         
             
             
         
      
     
     A stirred solution of 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(2-methoxy-ethyl)-urea (31 mg, example 82) and benzoic acid 4-chlorosulfonyl-phenyl ester (37 mg) in acetone (0.6 ml) was treated with triethylamine (33 μl). After stirring at ambient temperature for 4 hours, the reaction mixture was evaporated. The residue was filtered and the filtrate evaporated. The residue was directly purified by LCMS triggered purification to give benzoic acid 4-{2-[3-(2-methoxy-ethyl)-ureido]-1H-benzoimidazol-5-yloxysulfonyl}-phenyl ester (7.2 mg) as a off-white solid Mass spectrum: 511 [M+H] + ; retention time=9.90 minutes. 
     Example 88 
     By using a method similar to that for the preparation of example 87, combining benzoic acid 4-chlorosulfonyl-phenyl ester with suitable 1-(6-hydroxy-1H-benzoimidazol-2-yl)-urea (example 83, 84) were obtained the following compounds that were characterized by analytical LC/MS ([M+H] +  and retention time given in the following table). 
     
       
         
               
               
               
               
               
             
           
               
                   
               
               
                 Example 
                 1-(6-hydroxy-1H-benzoimidazol-2-yl)-urea 
                 Compound 
                 Retention time (minutes) 
                 Masse [M + H]+ 
               
               
                   
               
             
             
               
                 88-a 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 Benzoic acid 4-{2-[3-(2-morpho-lin-4-yl-ethyl)-urei-do}-1H-benzoimidazol-5-yl-oxysulfonyl}-phenylester 
                 7.44 
                 566 
               
               
                   
               
               
                 88-b 
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 Benzoic acid 4-[2-([3-pyri-din-2-ylmethyl)-ureido)-1H-benzo-imidazol-5-yl-oxysulfonyl]-phenyl ester 
                 8.91 
                 544 
               
               
                   
               
             
          
         
       
     
     Example 89(a) 
     Preparation of 2,6-difluoro-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzoimidazol-5-yl ester 
     
       
                 
         
             
             
         
      
     
     A stirred solution of 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-pyridin-2-ylmethyl-urea (50 mg, example 83-a) and 2,6-difluoro-benzene-sulfonyl chloride (38 mg) in acetone (1 ml) was treated with triethylamine (48 μl). After stirring at ambient temperature for 4 hours, the reaction mixture was evaporated. The residue was filtered and the filtrate evaporated. The residue was directly purified by LC/MS triggered purification to give benzoic acid 2,6-difluoro-benzenesulfonic acid 2-(3-pyridin-2-ylmethyl-ureido)-3H-benzoimidazol-5-yl ester (29.6 mg) as a off-white solid Mass spectrum: 427 [M+H] + ; retention time=7.86 minutes. 
     Example 89(b) 
     Preparation of 2,6-difluoro-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzoimidazol-5-yl ester 
     By proceeding in a manner similar to example 89(a) above but using 1-(6-hydroxy-1H-benzoimidazol-2-yl)-3-(2-methoxy-ethyl)-urea there was prepared 2,6-difluoro-benzenesulfonic acid 2-[3-(2-methoxy-ethyl)-ureido]-3H-benzoimidazol-5-yl ester as an off-white solid. Mass spectrum: 427 [M+H] + ; retention time=7.86 minutes. 
     Biological Tests 
     The experiments described in this report were designed to evaluate the cytotoxicity of “in vitro” Cdk4 inhibitors in comparison with Staurosporine, a non-specific Serine-Threonine kinase inhibitor. 
     Stock solutions of compounds were made in DMSO at 10 mM and stored at −20° C. Subsequent dilutions were made in 28% DMSO and used to add 3 μl of the drugs at varied concentrations to the HeLa cells. 
     All cell lines were cultured at 37° C. in a humidified atmosphere containing 5% CO 2 . 
     HeLa human epithelial cell line was obtained from the American Type Culture Collection (Rockville, Md., USA). Cells were grown as monolayers in Dubelcco&#39;s Modified Eagle Medium containing 2 mM L-glutamine, 200 I.U./ml penicillin, 200 μg/ml streptomycin, and supplemented with 10% (v/v) heat inactivated foetal calf serum. Cells were transferred twice a week at 10 5  cells/ml in 75 cm 2  flasks after trypsinisation. Different flasks were done to prepare two preparations the day of experiment. 
     Cell Growth Inhibition 
     Cells in exponential phase of growth were trypsinised and resuspended in their culture medium at 2.5 10 4  cells/ml, in two independent preparations. Cell suspension was distributed in 96 well Cytostar microplates (Amersham) (0.2 ml/well, 5000 cells). Hela cells were coated for 4 hours at 37° C. [ 14 C]-thymidine (0.1 μCi/well) and ten final concentrations of molecules (3 μl) ranging from 20 to 0.03 μM were then added. The uptake of [ 14 C]-thymidine was measured 48 h after the labelling had been started using a Microbeta Trilux counter (Wallac). 
     Staurosporine, the reference compound, was evaluated using the same procedure. 
     CPM measured 48 hours after the test substance had been added to the media, were compared to those obtained with 0.4% final DMSO, in the control wells. 
     IC 50 , obtained from a dose response curve of 10 concentrations in duplicate is the concentration of drug wich diminishes half the specific signal. It is determined by non-linear regression analysis and calculated as a concentration at middle of curve. 
     IC 50  values result from 2 independent experiments for all tested molecules. 
     CDK4/CyclinD1 Flashplate Assay: 96-well Format 
     This is a CDK4/CyclinD1 kinase assay in a 96-well Streptavidin-coated Flashplate with a biotinylated-Rb peptide substrate. 
     Each point is tested in duplicate 
     Biotinylated-Rb: Biotin-RPPTLSPIPHIPRSPYKFPSSPLR 
     
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Kinase Buffer: 
                   
               
               
                   
                   
               
             
             
               
                   
                 HEPES, pH 8 
                 50 mM 
               
               
                   
                 MgCl 2  6H 2 O, pH 7 
                 10 mM 
               
               
                   
                 DTT 
                  1 mM 
               
               
                   
                   
               
             
          
         
       
         
         1. Prepare substrate: 1 mg/ml solution made fresh in PBS. 
         2. Add 100 μg per well to the Flashplate. 
         3. Incubate for 2 hours at RT. 
         4. From 10 mM inhibitor stocks in DMSO, make 1 mM, 300 μM, 100 μM, 30 μM and 10 μM series of dilution in DMSO. 
         5. Wash the Flashplate 3 times with 300 μl PBS to remove unbound peptide substrate. 
         6. Add the CDK4/CyclinD1 kinase: 70 ng per well, in a volume of 90 μl in kinase buffer (except for “no enzyme” control wells). 
         7. Add 1 μl 1  per well of inhibitor to test 10 μM, 3 μM, 1 μM, 0.3 μM and 0.1 μM in final concentration per 100 μl in each well. 
         8. Shake gently the Flashplate 1 minute. 
         9. Incubate 30 minutes on wet ice. 
         10. Initiate the reaction with 10 μl kinase buffer containing 1 μM final cold ATP and 1 μCi final  33 P-ATP per well. 
         11. Shake gently the Flashplate 1 minute. 
         12. Incubate 45 minutes at RT (no shaking). 
         13. Wash the Flashplate 3 times with 300 μl PBS 
         14. Count to detect the incorporation of  33 P-ATP by the kinase to the Rb phosphorylation site. 
       
    
     CDK2/CyclinE Flashplate Assay: 96-well Format 
     This is a CDK2/CyclinE kinase assay in a 96-well Streptavidin-coated Flashplate with a biotinylated-Rb peptide substrate. 
     Each Point is Tested in Duplicate 
     Biotinylated-Rb: Biotin-SACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTR-OH 
     
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Kinase Buffer: 
                   
               
               
                   
                   
               
             
             
               
                   
                 HEPES, pH 8.0 
                 50 mM 
               
               
                   
                 MgCl 2  6H 2 O 
                 10 mM 
               
               
                   
                 DTT 
                  1 mM 
               
               
                   
                   
               
             
          
         
       
         
         1. Prepare substrate: 1 mg/ml solution made fresh in PBS. 
         2. Add 4 μg per well to the Flashplate. 
         3. Incubate for 2 hours at RT. 
         4. From 10 mM inhibitor stocks in DMSO, make 1 mM, 300 μM, 100 μM, 30 μM and 10 μM series of dilution in DMSO. 
         5. Was the Flashplate 3 times with 300 μl PBS to remove unbound peptide substrate. 
         6. Add the CDK2/CyclinE kinase: 200 ng per well, in a volume of 90 μl in kinase buffer (except for “no enzyme” control wells). 
         7. Add 1 μl per well of inhibitor to test 10 μM, 3 μM, 1 μM, 0.3 μM and 0.1 μM in final concentration per 100 μl in each well. 
         8. Shake gently the Flashplate 1 minute. 
         9. Incubate 30 minutes on wet ice. 
         10. Initiate the reaction with 10 μl kinase buffer containing 1 μM final cold ATP and 1 μCi final  33 P-ATP per well. 
         11. Shake gently the Flashplate 1 minute. 
         12. Incubate 45 minutes at RT (no shaking). 
         13. Wash the Flashplate 3 times with 300 μl PBS 
         14. Count to detect the incorporation of  33 P-ATP by the kinase to the Rb phosphorylation site. 
       
    
     
       
         
               
               
               
               
             
               
               
               
               
             
           
               
                   
                   
               
               
                   
                   
                 IC50 CDK4/cyclinD1 
                 IC50 CDK2/cyclinE 
               
               
                   
                 Example N° 
                 (μM) 
                 (μM) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                  1 
                 1.5 
                 0.6 
               
               
                   
                  2 
                 2 
                 0.7 
               
               
                   
                  3 
                 2.4 
                 0.5 
               
               
                   
                  4 
                 6.3 
                 1.5 
               
               
                   
                  5 
                 1.12 
                 2.2 
               
               
                   
                  6 
                 0.84 
                 0.3 
               
               
                   
                  7 
                 0.47 
                 2 
               
               
                   
                  8 
                 1.1 
                 Nd 
               
               
                   
                  9 
                 2 
                 Nd 
               
               
                   
                 10 
                 0.7 
                 0.8 
               
               
                   
                 11 
                 0.93 
                 0.5 
               
               
                   
                 12 
                 14% inhibition at 10 μM 
                 Nd 
               
               
                   
                 13 
                 0.4 
                 2 
               
               
                   
                 14 
                 0.3 
                 0.2 
               
               
                   
                 15 
                 0.3 
                 1.8 
               
               
                   
                 16 
                 0.37 
                 1.8 
               
               
                   
                 17 
                 6.3 
                 2 
               
               
                   
                 18 
                 1.3 
                 0.6 
               
               
                   
                 19 
                 2.92 
                 0.7 
               
               
                   
                 20 
                 &gt;3 
                 &gt;10 
               
               
                   
                 22 
                 1.77 
                 Nd 
               
               
                   
                 23 
                 3.1 
                 0.4 
               
               
                   
                 24 
                 0.6 
                 0.4 
               
               
                   
                 25 
                 0.13 
                 0.08 
               
               
                   
                 26 
                 0.68 
                 0.13 
               
               
                   
                 27 
                 0.6 
                 0.042 
               
               
                   
                 28 
                 1.03 
                 0.6 
               
               
                   
                 29 
                 1.7 
                 0.6 
               
               
                   
                 30 
                 1.8 
                 0.9 
               
               
                   
                 31 
                 0.5 
                 0.1 
               
               
                   
                 32 
                 &gt;5 
                 1.1 
               
               
                   
                 33 
                 0.64 
                 0.06 
               
               
                   
                 34 
                 1.18 
                 0.12 
               
               
                   
                 35 
                 1.1 
                 0.12 
               
               
                   
                 36 
                 0.77 
                 0.53 
               
               
                   
                 37 
                 0.57 
                 0.45 
               
               
                   
                 38 
                 1.25 
                 4.3 
               
               
                   
                 39 
                 1.62 
                 0.29 
               
               
                   
                 40 
                 3.71 
                 1.09 
               
               
                   
                 41 
                 2.8 
                 0.06 
               
               
                   
                 42 
                 84% inhibition at 10 μM 
                 0.91 
               
               
                   
                 43 
                 1.9 
                 0.2 
               
               
                   
                 44 
                 O.6 
                 0.13 
               
               
                   
                 45 
                 0.6 
                 0.06 
               
               
                   
                 46 
                 0.012 
                 0.06 
               
               
                   
                 47 
                 0.8 
                 0.16 
               
               
                   
                 48 
                 0.3 
                 0.04 
               
               
                   
                 49 
                 88% inhibition at 10 μM 
                 0.4 
               
               
                   
                 50 
                 0.3 
                 0.04 
               
               
                   
                 51 
                 0.8 
                 0.14 
               
               
                   
                 52 
                 1 
                 0.08 
               
               
                   
                 53 
                 83% inhibition at 10 μM 
                 0.8 
               
               
                   
                 54 
                 0.5 
                 0.005 
               
               
                   
                 57-a 
                 1 
                 0.24 
               
               
                   
                 57-b 
                 51% inhibition at 10 μM 
                 1.9 
               
               
                   
                 57-c 
                 60% inhibition at 10 μM 
                 0.6 
               
               
                   
                 57-d 
                 70% inhibition at 10 μM 
                 0.5 
               
               
                   
                 57-e 
                 90% inhibition at 10 μM 
                 0.3 
               
               
                   
                 57-f 
                 88% inhibition at 10 μM 
                 0.5 
               
               
                   
                 57-g 
                 52% inhibition at 10 μM 
                 0.6 
               
               
                   
                 57-h 
                 4.4 
                 0.1 
               
               
                   
                 57-i 
                 27% inhibition at 10 μM 
                 5.2 
               
               
                   
                 57-j 
                 3 
                 0.1 
               
               
                   
                 57-k 
                 49% inhibition at 10 μM 
                 0.6 
               
               
                   
                 57-l 
                 1 
                 0.07 
               
               
                   
                 58-a 
                 96% inhibition at 10 μM 
                 0.38 
               
               
                   
                 58-b 
                 70% inhibition at 10 μM 
                 0.9 
               
               
                   
                 58-c 
                 60% inhibition at 10 μM 
                 0.6 
               
               
                   
                 58-d 
                 84% inhibition at 10 μM 
                 1.6 
               
               
                   
                 58-e 
                 0.1 
                 0.04 
               
               
                   
                 58-f 
                 91% inhibition at 10 μM 
                 0.7 
               
               
                   
                 58-g 
                 69% inhibition at 10 μM 
                 1 
               
               
                   
                 58-h 
                 3 
                 0.1 
               
               
                   
                 58-i 
                 81% inhibition at 10 μM 
                 0.3 
               
               
                   
                 58-j 
                 0.5 
                 0.009 
               
               
                   
                 58-k 
                 0.5 
                 0.04 
               
               
                   
                 58-l 
                 1 
                 0.03 
               
               
                   
                 58-m 
                 0.24 
                 0.03 
               
               
                   
                 58-n 
                 0.6 
                 Nd 
               
               
                   
                 58-o 
                 0.029 
                 0.02 
               
               
                   
                 58-p 
                 0.6 
                 Nd 
               
               
                   
                 58-q 
                 1 
                 Nd 
               
               
                   
                 58-r 
                 80% inhibition at 10 μM 
                 Nd 
               
               
                   
                 58-s 
                 0.012 
                 0.02 
               
               
                   
                 58-t 
                 9 
                 Nd 
               
               
                   
                 58-u 
                 0.29 
                 0.01 
               
               
                   
                 58-v 
                 0.11 
                 0.1 
               
               
                   
                 58-w 
                 0.19 
                 0.25 
               
               
                   
                 58-x 
                 0.31 
                 0.04 
               
               
                   
                 58-y 
                 0.27 
                 0.01 
               
               
                   
                 58-z 
                 2.23 
                 Nd 
               
               
                   
                 58-aa 
                 0.34 
                 0.1 
               
               
                   
                 58-ab 
                 0.22 
                 0.002 
               
               
                   
                 58-ac 
                 0.17 
                 0.013 
               
               
                   
                 58-ad 
                 0.13 
                 0.016 
               
               
                   
                 58-ae 
                 1.49 
                 Nd 
               
               
                   
                 58-af 
                 0.21 
                 0.18 
               
               
                   
                 58-ag 
                 0.39 
                 0.04 
               
               
                   
                 58-ah 
                 0.33 
                 0.03 
               
               
                   
                 58-ai 
                 0.33 
                 0.15 
               
               
                   
                 58-aj 
                 0.38 
                 0.37 
               
               
                   
                 58-ak 
                 0.18 
                 0.1 
               
               
                   
                 58-al 
                 0.25 
                 0.15 
               
               
                   
                 58-am 
                 0.24 
                 0.08 
               
               
                   
                 58-an 
                 0.2 
                 0.1 
               
               
                   
                 59-a 
                 0.1 
                 0.008 
               
               
                   
                 59-b 
                 0.3 
                 0.007 
               
               
                   
                 59-c 
                 1 
                 0.007 
               
               
                   
                 59-d 
                 0.5 
                 0.015 
               
               
                   
                 59-e 
                 1.6 
                 0.1 
               
               
                   
                 59-f 
                 1.8 
                 0.06 
               
               
                   
                 59-g 
                 1.8 
                 0.2 
               
               
                   
                 59-h 
                 1.1 
                 0.2 
               
               
                   
                 59-i 
                 1.2 
                 0.04 
               
               
                   
                 59-j 
                 0.9 
                 0.007 
               
               
                   
                 59-k 
                 0.3 
                 0.02 
               
               
                   
                 59-l 
                 0.3 
                 0.004 
               
               
                   
                 59-m 
                 1.2 
                 0.03 
               
               
                   
                 59-n 
                 0.13 
                 0.036 
               
               
                   
                 59-o 
                 0.8 
                 0.04 
               
               
                   
                 59-p 
                 0.18 
                 0.017 
               
               
                   
                 59-q 
                 0.75 
                 0.11 
               
               
                   
                 59-r 
                 1.8 
                 0.22 
               
               
                   
                 60-a 
                 0.8 
                 Nd 
               
               
                   
                 60-b 
                 0.15 
                 Nd 
               
               
                   
                 60-c 
                 0.34 
                 Nd 
               
               
                   
                 60-d 
                 0.9 
                 Nd 
               
               
                   
                 60-e 
                 0.9 
                 Nd 
               
               
                   
                 60-f 
                 1 
                 Nd 
               
               
                   
                 60-g 
                 1.5 
                 Nd 
               
               
                   
                 60-h 
                 Nd 
                 Nd 
               
               
                   
                 60-i 
                 0.3 
                 Nd 
               
               
                   
                 60-j 
                 Nd 
                 Nd 
               
               
                   
                 60-k 
                 Nd 
                 Nd 
               
               
                   
                 60-l 
                 0.4 
                 Nd 
               
               
                   
                 60-m 
                 0.5 
                 Nd 
               
               
                   
                 60-n 
                 2 
                 Nd 
               
               
                   
                 60-o 
                 5 
                 Nd 
               
               
                   
                 60-p 
                 1.2 
                 Nd 
               
               
                   
                 60-q 
                 1.8 
                 Nd 
               
               
                   
                 60-r 
                 0.6 
                 Nd 
               
               
                   
                 60-s 
                 8 
                 Nd 
               
               
                   
                 60-t 
                 2 
                 Nd 
               
               
                   
                 60-u 
                 Nd 
                 Nd 
               
               
                   
                 60-v 
                 4.2 
                 Nd 
               
               
                   
                 60-w 
                 0.5 
                 Nd 
               
               
                   
                 60-x 
                 5 
                 Nd 
               
               
                   
                 60-y 
                 2.5 
                 Nd 
               
               
                   
                 60-z 
                 3 
                 Nd 
               
               
                   
                 60-aa 
                 3.9 
                 Nd 
               
               
                   
                 60-ab 
                 4 
                 Nd 
               
               
                   
                 60-ac 
                 0.8 
                 Nd 
               
               
                   
                 60-ad 
                 1.3 
                 Nd 
               
               
                   
                 60-ae 
                 2.2 
                 Nd 
               
               
                   
                 60-af 
                 Nd 
                 Nd 
               
               
                   
                 60-ag 
                 Nd 
                 Nd 
               
               
                   
                 60-ah 
                 0.55 
                 Nd 
               
               
                   
                 60-ai 
                 1.6 
                 Nd 
               
               
                   
                 61 
                 2.06 
                 Nd 
               
               
                   
                 62-a 
                 50% inhibition at 10 μM 
                 Nd 
               
               
                   
                 62-b 
                 0.44 
                 0.2 
               
               
                   
                 62-c 
                 3 
                 0.8 
               
               
                   
                 62-d 
                 1.3 
                 Nd 
               
               
                   
                 62-e 
                 4.6 
                 1 
               
               
                   
                 62-f 
                 3 
                 1.5 
               
               
                   
                 62-g 
                 3 
                 1 
               
               
                   
                 62-h 
                 0.8 
                 0.07 
               
               
                   
                 62-i 
                 1.5 
                 1 
               
               
                   
                 62-j 
                 3 
                 1.5 
               
               
                   
                 62-k 
                 3 
                 Nd 
               
               
                   
                 62-l 
                 2.5 
                 Nd 
               
               
                   
                 62-m 
                 1.2 
                 Nd 
               
               
                   
                 62-n 
                 1 
                 Nd 
               
               
                   
                 62-o 
                 1 
                 Nd 
               
               
                   
                 62-p 
                 0.7 
                 Nd 
               
               
                   
                 62-q 
                 1.6 
                 Nd 
               
               
                   
                 62-r 
                 2.5 
                 Nd 
               
               
                   
                 62-s 
                 1.75 
                 Nd 
               
               
                   
                 63 
                 1.5 
                 Nd 
               
               
                   
                 64-a 
                 1 
                 Nd 
               
               
                   
                 64-b 
                 50% at 10 μM 
                 Nd 
               
               
                   
                 64-c 
                 4.37 
                 Nd 
               
               
                   
                 64-d 
                 50% at 10 μM 
                 Nd 
               
               
                   
                 64-e 
                 Nd 
                 Nd 
               
               
                   
                 65 
                 Nd 
                 1 
               
               
                   
                 66-a 
                 19% inhibition at 10 μM 
                 Nd 
               
               
                   
                 66-b 
                 3.05 
                 Nd 
               
               
                   
                 66-c 
                 Nd 
                 1 
               
               
                   
                 67 
                 91% inhibition at 10 μM 
                 Nd 
               
               
                   
                 68-a 
                 97% inhibition at 10 μM 
                 Nd 
               
               
                   
                 68-b 
                 78% inhibition at 10 μM 
                 Nd 
               
               
                   
                 69 
                 0.84 
                 0.13 
               
               
                   
                 70-a 
                 1.18 
                 Nd 
               
               
                   
                 70-b 
                 0.67 
                 3 
               
               
                   
                 70-c 
                 2 
                 0.8 
               
               
                   
                 70-d 
                 0.65 
                 0.3 
               
               
                   
                 70-e 
                 0.5 
                 0.8 
               
               
                   
                 70-f 
                 Nd 
                 5 
               
               
                   
                 70-g 
                 5 
                 1.5 
               
               
                   
                 70-h 
                 0.14 
                 0.25 
               
               
                   
                 78-e 
                 0.6 
                 Nd 
               
               
                   
                 78-f 
                 0.1 
                 Nd 
               
               
                   
                 78-h 
                 90% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-i 
                 97% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-k 
                 78% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-l 
                 100% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-n 
                 54% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-o 
                 54% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-q 
                 94% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-s 
                 88% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-t 
                 55% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-u 
                 93% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-v 
                 46% inhibition at 10 μM 
                 Nd 
               
               
                   
                 78-w 
                 90% inhibition at 10 μM 
                 Nd 
               
               
                   
                 80-c 
                 100% inhibition at 10 μM 
                 Nd 
               
               
                   
                 80-m 
                 103% inhibition at 10 μM 
                 Nd 
               
               
                   
                 82-h 
                 92% inhibition at 10 μM 
                 Nd