Abstract:
Otitis Externa is treated in dogs by administering to the infected animal a dosage effective to alleviate the symptoms of the infection of a composition of beta-(1-adamantyl)-alpha,alpha-dimethylethylamine or its acid salt and at least one pharmaceutically acceptable carrier wherein the amine (or its salt) is from about 0.01 to about 95% by weight of the composition.

Description:
BACKGROUND OF THE INVENTION 
     The invention relates to a method of treating Otitis externa in dogs with a composition of beta-(1-adamantyl)-alpha,alpha-dimethylethylamine or its acid salt, preferably the hydrochloride and at least one pharmaceutically acceptable carrier. 
     Otitis externa, an inflammation of the external auditory meatus, is a common disease in dogs. Although the disease is not fatal, it is aesthetically disagreeable to the owner and causes discomfort to the affected dogs and may result in internal ear infection and deafness. The microbial flora of the ear canal of dogs suffering from this disease has been studied by several workers, but results of these studies and conclusions differ widely. Although the etiology of Otitis Externa in the dog remains poorly defined, the most frequent microorganisms found in dogs affected with this disease are blastomyces, staphylococci, pytyrosporum canis, pseudomonas, proteus and prototheca wickerhammii. The highest percentage of dogs having this disease tends to be among the breeds of dogs with pendulous ears and long hair; the disease in these dogs sometimes spreads to the neck and head areas that the ears are in constant contact. Adult dogs tend to contract the disease more than others. (see V. D. Sharma and H. E. Rhoads, J. Small Animal Pract., 16, pp. 241 to 247 (1957)). 
     Prior to the instant invention, treatment of Otitis Externa was based on the prolonged deep instillation of antibiotics, antifungals, corticosteroids, and proteolytic enzymes. In general, many of the dogs treated in this manner did not respond well to the treatment and had to be retreated. Beta-(1-adamantyl)-alpha,alpha-dimethylethylamine has now been found to treat dogs successfully for Otitis Externa. 
     No prior art is known which discloses the use of beta-(1adamantyl)-alpha,alpha-dimethylethylamine for the treatment of Otitis Externa in dogs. 
     U.S. Pat. Nos. 3,270,036, 3,489,802, and 3,501,511 teach various adamantylamine and adamantylalkylamine derivatives which have a number of valuable pharmaceutical characteristics such as being used as hypoglycemic agents and antiviral agents. 
     U.S. Pat. No. 4,100,170 teaches adamantylethylamines that are useful as anorexic agents. 
     SUMMARY OF THE INVENTION 
     This invention is directed to a method for treating Otitis Externa in dogs comprising administering to dogs a dosage effective to alleviate the symptoms of the infection of a composition of beta-(1-adamantyl)-alpha,alpha-dimethylethylamine or its acid salt and at least one pharmaceutically acceptable carrier, wherein the amine (or its salt) is from about 0.01 to about 95% by weight of the composition. 
     The identity of the acid forming the salt of the amine of the composition is not critical. The hydrochloride of the amine is preferred, but any pharmaceutically acceptable inorganic or organic acid such as the sulfate, acetate, nitrate or the like may be used. 
     DETAILED DESCRIPTION OF THE INVENTION 
     The drug of this invention can be administered in the microbial and fungal disease treatment according to this invention by any means that effects contact of the active ingredient compound with the site of infection on the dog. For example, a dosage form of the drug may be used for oral, parenteral, topical, aural, or rectal application. The dosage form may be a solution, gel, emulsion, suspension, paste, ointment, suppository, tablet, capsule, powder, granule or an aerosol product or other suitable formulation. The dosage administered will be dependent upon the weight of the dog and the frequency of the required treatment. Generally, a daily topical dosage of active ingredient will be from about 1 to 50 milligrams per kilogram of the dog&#39;s body weight, although lower and higher amounts can be used. The active ingredient, the drug, can be employed in useful compositions according to the present invention in such dosage forms as solution, semisolid, solid, and aerosol forms. These dosage forms preferably deliver from about 1 mg to about 500 mg of active ingredient, with the range from about 10 mg to about 200 mg being most preferred. In these dosage forms the composition will contain at least one non-toxic pharmaceutically acceptable carrier for the active ingredient. Examples of the non-toxic carriers or adjuvants are viscosity enhancers such as bentonite, celluloses (e.g., methylcellulose, ethylcellulose, and carboxy methylcellulose) and tragacanth; pH modifiers such as dibasic sodium phosphate, citric acid, and sodium hydroxide; preservatives such as methyl paraben, propyl paraben, benzoic acid, and benzyl alcohol; sweeteners such as saccharin, sorbitol (D-glucitol), and mannitol; stability enhancers such as sodium bisulfite and ascorbic acid; coloring agents such as food, drug and cosmetic (FD&amp;C) and drug and cosmetic (D&amp;C) colors certified by the Food and Drug Administration (FDA); solvents such as water, alcohol (e.g., ethyl alcohol (for internal use) and isopropyl alcohol (for external use)), and propylene glycol; suspending agents such as kaolin, celluloses (e.g. methylcellulose, ethylcellulose and carboxy methylcellulose), acacia, and tragacanth; granulating agents such as acacia, sucrose, and polyvinylpyrrolidone (PVP); coating agents such as celluloses (e.g., ethylcellulose and propylcellulose) and PVP; disintegration/dissolution modifiers such as starch (e.g., corn starch, rice starch and potato starch) and polysorbate 80 (polyoxyethylene (20) sorbitan monooleate); excipients such as lactose, starch, and cellulose; propellents such as isobutane, fluorocarbon 11 (trichlorofluoro methane), fluorocarbon 12 (dichlorodifluoro methane), and ion exchange agents such as XE-69 and IR 120 (sulfonic acid cation resins (styrene divinyl benzene)) and IRP 58 (a phenolic polyamine anion exchange resin); emulsifying agents such as glyceryl stearate (self emulsifying), sorbitan stearate, decyl oleate, cetearyl alcohol, polysorbate 60 and triethanolamine; and humectants such as myristyl myristate. 
     Typical embodiments of the pharmaceutical composition of this invention are: (all percentages are by weight of composition) 
     
         ______________________________________1.   Tablet:drug                       100 mgmicrocrystalline cellulose 100 mgmagnesium stearate         5 mg2.   Capsule:drug                       100 mglactose                    100 mgstarch                     5 mgmagnesium stearate         2 mg3.   Oral Solution:drug                       2 gsorbitol (D-glucitol) solution 70%                           50 mlcitrus flavor              1 gcitric acid                1 gdistilled water, quantity sufficient tomake (q.s. ad)             100 ml4.   Parenteral Solution:drug                       2.5 gbenzyl alcohol             0.1 gsterile distilled water, q.s. ad                           100 ml5.   Aerosol Spray:drug                       5%polysorbate 20 (polyoxyethylene 20sorbitan monolaurate)      0.1%propellent 11/12, 40/60 ratio byweight, q.s. ad            100%6.   Topical Lotion:drug                       5%mineral oil                30%polysorbate 80             10%propylene glycol           5%white petrolatum           5%benzoic acid               2%perfume                    0.1%water q.s. ad              100%7.   Topical Ointment:drug                       5%hydrophilic ointment U.S.P. q.s. ad                           100%8.   Oral Resinated Suspension (sustained release):drug resinate              10%(drug content of resin is  15%)keltrol (xanthan gum)      10%saccharin                  0.5%flavor                     0.2%sorbitol 70% solution      50%methylparaben              0.5%water, q.s. ad             100%9.   Oral Resinated Capsule (sustained release):drug resinate              200 mg(drug content of resin is  50%)lactose 100 mgmagnesium stearate         5 mg10.  Topical Gel:drug                       5%carbopol 934 (carboxypolymethylene)                           1%alcohol                    10%triethanolamine            0.8%polyethylene glycol 300water q.s. ad              100%______________________________________ 
    
     Note that &#34;drug&#34; in each of the above examples is beta-(1-adamantyl)-alpha,alpha-dimethylethylamine or its acid salt. 
    
    
     EXAMPLE 
     Beta-(1-Adamantyl)-alpha,alpha-dimethylethylamine hydrochloride (I) was tested for its antimicrobial and antifungal activity against Otitis Externa in dogs using the following method. Dogs A and B, which were diagnosed to have Otitis Externa, had their ears washed with Ivory soap and were treated with a 4% aqueous solution of (I) once a day; the drug was applied topically to the affected ears in the following aqueous solution: 
     
         ______________________________________Compound (I)         4 g/100 ml as baseIsopropyl alcohol    32 ml/100 mlPolyethylene glycol 200                8 ml/100 mlD.I. water q.s. ad   100 ml.______________________________________ 
    
     Within three days both dogs showed noticeable improvement. The redness in the ears disappeared, and the ulcerations began to heal. The ears appeared generally dryer, and the erythema and edema disappeared. 
     After eight days of treatment, dog A had almost completely recovered. His ears were just slightly red, and each ear had one small abscess but the rest of the lesions and ulcerations had completely healed; the hair on the ears had started to grow back. By the sixteenth day, dog A was completely healed and all his hair had grown in. Dog A was examined every day for a further month and did not show any signs of recurrence of the disease. 
     After eight days of treatment, dog B had also almost completely recovered; the swelling in the ears had disappeared and most of the open ulcerations had healed while the few ulcerations that remained were dry and in the last stages of healing. The ruffled edges of the ear flopes were disappearing also. The application of the 4% solution to the dog&#39;s ears was stopped after 22 days. On the fourteenth day, however, it was observed that dog B also had similar lesions on his neck. Hence, dog B&#39;s neck was clipped, washed with Ivory soap, and then treated with the above-mentioned 4% aqueous solution of compound (I), which was applied topically to the affected neck for 17 days. The neck lesions completely disappeared. Dog B was examined for an additional two weeks with no signs of the disease recurring.