Abstract:
The present invention relates to a novel crystalline polymorph form W of cabazitaxel and to method for the preparation thereof.

Description:
[0001]    The present application claims priority to Chinese Patent Application No. 201310440458.4, filed on Sep. 24, 2013. 
       BACKGROUND OF THE INVENTION 
       [0002]    Cabazitaxel belongs to the taxane class and is closely related in both chemical structure and mode of action to the anticancer drugs paclitaxel and docetaxel. Cabazitaxel, chemically known as (2α, 5β7β, 10β, 13α)-4-acetoxy-13({2,3S)-3-[(tert-butoxycarbonyl)amino]-2-hydroxy -3 -phenylpropanoyl}oxy)-1-hydroxy-7,10-dimethoxy-9-oxo-5,20-epoxy-tax -11-en-2-yl benzoate, is represented by formula (I). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0003]    Cabazitaxel is microtuble inhibitor, which can promote the formation of tubulin by combined with tubulin protein, while cabazitaxel also an prevent the mierotubule assembled to disassemble. 
         [0004]    Crystalline form is one of the important factors to affect the drug quality, drug efficacy and performance of pharmaceutic preparation. In view of the good effect of cabazitaxel, the people have made a lot of research on it and developed many crystalline form. Finally, crystalline acetone solvate form of (2α, 5β, 7β, 10β, 13α)-4-acetoxy -13-({2,3 S)-3-[(tert-butoxycarbonyl)amino]-2-hydroxy-3phenylpropanoyl}oxy)-1-hydroxy-7,10-dimethoxy-9-oxo-5,20-epoxy-tax-11-en-2-yl benzoate is known and described in WO2005028462, which is named crystalline form A. Followed crystalline forms B, C, D, E, F are known and described in Chinese patent CN101918385, which are all converted from form A. While crystalline form B, D, E, F ethanol solvates are also known and described in patent CN101918385. 
         [0005]    In summary, as we know, the preparation of crystalline forms of cabazitaxel needs specific environment to realize, which is had to industrialize. Further more, in the process of the preparation of crystalline form, we can find high temperature has been used to remove the solvent which makes the purity of the product to decrease. It is also known that the ability of a substance to exist in more than one crystal form is defined as polymorphism and its different crystal forms are called polymorphs. Polymorphs will affect the solubility, stability and bioavailability of drug, furthermore it will affect the quality, safety, effectiveness and application of drug. 
       BRIEF SUMMARY OF THE INVENTION 
       [0006]    In one aspect, the present invention provides a crystalline form of cabazitaxel. The novel form have chemically characterized by  1 HNMR (nuclear magnetic resonance) spectroscopy, XRPD, FTIR (Fourier transform infrared) spectroscopy (also abbreviated to IR spectroscopy), TGA (theramgravimetric analysis) DSC (differential scanning calorimetry). 
         [0007]    In second aspect, the present invention provides the method of preparations for the novel crystal line form. 
         [0008]    In third aspect, the present invention provides process for the preparation of the crystalline form W of cabazitaxel. In some embodiments, the inventive process includes: 
         [0009]    a) any kind of crystalline form of cabazitaxel dissolves in appropriate organic solvent, appropriate ionized water is added slowly, after finished dropwise the solution is kept at 20-25° C. for 16 hours, then colorless crystal formed; 
         [0010]    b) filtering the solid and washed the solid with methanol (50%); 
         [0011]    c) drying the isolated and washed solid resulting from step b) under vacuum, at 40-50° C.; 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0012]      FIG. 1  shows the XRPD pattern for cabazitaxel Form W. 
           [0013]      FIG. 2  shows the DCS trace of cabazitaxel Form W. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0014]    The present invention provides novel crystalline form W of cabazitaxel. The crystalline forms can be produced by method described herein in substantially pure form, i.e., at least with 80% purity or higher. Form W can be produced with a purity of at least 90%, preferably at least 95% and more preferably at least 98% and the most preferably at least 99%. 
         [0015]    In one aspect, the present invention provides a crystalline form of (2α, 5β, 7β, 10β, 13α)-4-acetoxy-13-({(2,3 S)-3-[(tert,butoxycarbonyl)amino]-2-hydroxy-3-phenylpropanoyl}oxy)-1-hydroxy-7, 10-dimethoxy-9-oxo-5,20-epoxy-tax-11-en-2-yl benzoate. 
         [0016]    The crystalline compound of the present invention can be characterized by a number of techniques including X-ray power diffraction (XRPD), infrared spectroscopy (IR), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), and crystallography. 
         [0017]    In some embodiments, the present invention provides the crystalline form of the compound characterized by an XRPD pattern substantially in accordance with that of  FIG. 1 . 
         [0018]    In other embodiments, the crystalline form of the compound is Form W. characterized by XRPD pattern that includes one or more peaks at 4.4, 7.8, 8.5, 11.4, 12.8 15.3, 17.0, 20.4, 21.4, 22.5, 23.4, 27.8, 29.7, 29.9, 33.3 and 34.3 degrees 2θ (±0.2 degrees 2θ). Wherein said XRPD patter is made using CuKα 1  radiation. Preferably, the XRPD pattern shall have at least the following peaks 4.4, 8.5, 17.0 and 21.4 degrees. 
         [0019]    Crystalline Form W of the present invention is also characterized by a DSC substantially in accordance with  FIG. 2 . 
         [0020]    In a related aspect, the present invention provides a process for preparing crystalline Form W of cabazitaxel including: 
         [0021]    a.) any kind of crystalline form of cabazitaxel dissolves in appropriate organic solvent, appropriate ionized water is added slowly, after finished drop wise the solution is kept at 20-25° C. for 16 hours, then colorless crystal formed; 
         [0022]    b) filtering the solid and washed the solid with methanol (50% aqueous); 
         [0023]    c) drying the isolated and washed solid resulting from step b) under at 40-50° C. 
         [0024]    The organic solvent above-mentioned include methanol, ethanol, isopropanol and acetonitrile etc. Appropriate ionized water is added slowly, while keep the volume radio of organic solvent and water at the range of 55:45-85:15. The temperature of the solution must be kept at 15-25° C. during the crystallizing. The drying time should be kept below 24 hours and the temperature should be kept at 40-50° C. 
         [0025]    The present invention will be described more fully by means a the following examples which should not be considered to limit the invention. 
         [0000]    Experimental analysis conditions:
 
Differential scanning calorimetry (DSC):
 
         [0026]    The measurements were carried out on a T.A. The sample is subjected to temperature programming from 30° C. to 300° C. with a heating rate of 5° C./min. The product was placed in a crimped aluminum capsule and the amount of product analyzed is between 2 and 5 mg. 
       Power X-ray Diffraction (PXRD) 
       [0027]    The analyses were carried out on a Panalytical X&#39;Pert Pro diffractometer with a reflection-mode Braff-Brentano focusing geometry (θ-2θ) assembly. The product analyzed is deposited as a thin layer on a silicon single crystal. A copper anticathode tube (45 kV/40 mA) supplies an incident radiation Cu-Kα 1 ) (λ=1.54056). The beam is collimated using Sollers slits which improve the parallelism and variable slits which limit scattering. An X&#39;celerator detector completes the device. The diagram recording characteristics are the following: sweeping from 2 to 40 degree, 0.01°/1sec. 
       EXAMPLES 
       [0028]    The following examples are provided to further illustrate, but not to limit this invention. 
       Example 1 
       [0029]    Cabazitaxel (500 mg) was dissolved in 10 mL of methanol, and then ionized water (5 mL) was dropwised into it with stirring. Then the solution was kept at 15-25° C. for 16 hours. Then the mixture was filtered, and the collected solids were washed with methanol (50% aqueous) and dried in vacuum at 40° C. for 24 hours to give cabazitaxel Form W (490 mg, purity: 99.6%) as colorless solid (melting point 153.78 ° C). 
       Example 2 
       [0030]    Cabazitaxel (500 mg) was dissolved in 10 mL of ethanol, and then ionized water (5 mL) was dropwised into it with stirring. Then the solution was kept at 15-25° C. for 16 hours. Then the mixture was filtered, and the collected solids were washed with ethanol (50% aqueous) and dried in vacuum at 40° C. for 24 hours to give cabazitaxel Form W (492 mg, purity: 99.2%) as colorless solid. 
       Example 3 
       [0031]    Cabazitaxel (500 mg) was dissolved in 10 mL of IPA, and then ionized water (5 mL) was dropwised into it with stirring. Then the solution was kept at 15-25° C. for 16 hours. Then the mixture was filtered, and the collected solids were washed with IPA (50% aqueous) and dried in vacuum at 40° C. for 24 hours to give cabazitaxel Form W (460 mg, purity: 99.3%) as colorless solid. 
       Example 4 
       [0032]    Cabazitaxel (500 mg) was dissolved in 10 mL of acetonitrile, and then ionized water (5 mL) was dropwised into it with stirring. Then the solution was kept at 15-25° C. for 16 hours. Then the mixture was filtered, and the collected solids were washed with acetonitrile (50% aqueous) and dried in vacuum at 40-50° C. for 24 hours to give cabazitaxel Form W (482 mg, purity: 98.9%) as colorless solid. 
         [0033]    powder X-ray diffraction and DSC data on form W is summarized in the following Table 1 and compared with existing polymorphs: 
         [0000]    
       
         
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 Form 
                 Patent 
                 Characteristic peak 
                 mp (° C.) 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Anhydrous B 
                 CN101918385A 
                 7.3, 8.1, 9.8, 10.4, 11.1, 12.7, 13.1, 14.3, 15.4 
                 150 
               
               
                 Anhydrous C 
                 CN101918385A 
                 4.3, 6.8, 7.4, 8.7, 10.1, 11.1, 11.9, 12.3, 12.6, 
                 146 
               
               
                   
                   
                 13.1 
               
               
                 Anhydrous D 
                 CN101918385A 
                 3.9, 7.7, 7.8, 7.9, 8.6, 9.7, 10.6, 10.8, 11.1, 
                 175 
               
               
                   
                   
                 12.3 
               
               
                 Anhydrous E 
                 CN101918385A 
                 7.1, 8.1, 8.9, 10.2, 10.8, 12.5, 12.7, 13.2, 13.4, 
                 157 
               
               
                   
                   
                 13.9 
               
               
                 Anhydrous F 
                 CN101918385A 
                 4.4, 7.2, 8.2, 8.3, 8.8, 9.6, 10.2, 10.9, 11.2, 12.1 
                 148 
               
               
                   
                   
                 12.3 
               
               
                 Ethanol 
                 CN101918385A 
                 7.3, 7.8, 8.8, 10.2, 12.6, 12.9, 13.4, 14.2, 14.7, 
                 N/A 
               
               
                 solvate B 
                   
                 15.1 
               
               
                 Ethanol 
                 CN101918385A 
                 3.8, 7.5, 7.7, 8.4, 9.4, 10.3, 10.5, 11.1, 11.5, 
                 N/A 
               
               
                 solvate D 
                   
                 11.9 
               
               
                 Ethanol 
                 CN101918385A 
                 7.1, 8.1, 8.8, 10.2, 10.7, 12.5, 13.2, 13.4, 13.9, 
                 N/A 
               
               
                 solvate E 
                   
                 14.2 
               
               
                 Ethanol water 
                 CN101918385A 
                 4.4, 7.2, 8.2, 8.3, 8.8, 9.6, 10.3, 10.9, 11.2, 
                 N/A 
               
               
                 F 
                   
                 12.2 
               
               
                 Monohydrate 
                 CN101918385A 
                 4.3, 6.8, 7.4, 8.6, 10.1, 11.1, 11.9, 12.2, 12.6, 
                 N/A 
               
               
                 C 
                   
                 13.3 
               
               
                 Dihydrate C 
                 CN101918385A 
                 4.2, 6.9, 7.5, 8.4, 9.9, 10.9, 11.7, 12.3, 12.6, 
                 N/A 
               
               
                   
                   
                 13.2 
               
               
                 Anhydrous 
                 CN102675257A 
                 4.3, 7.1, 8.7, 10.2, 10.9, 12.2, 13.8, 15.2, 16.4, 
                 N/A 
               
               
                 solvate 
                   
                 17.0, 17.6, 18.3, 19.2, 19.6, 20.3 
               
               
                 Ester solvate 
                 CN102746258A 
                 7.9, 8.5, 10.1, 12.6, 14.0, 15.0, 15.8, 17.3, 
                 N/A 
               
               
                 J 
                   
                 19.4, 20.1 
               
               
                 Hydrate G 
                 CN102746258A 
                 4.5, 8.5, 8.9, 11.1, 12.4, 13.9, 15.4, 17.7, 19.3 
                 N/A 
               
               
                 Form 1 
                 CN102746258A 
                 7.4, 7.8, 8.9, 10.1, 14.4, 15.0, 15.7, 17.7, 19.6, 
                 N/A 
               
               
                   
                   
                 23.5 
               
               
                 IPA solvate 
                 WO2013069027 
                 7.4, 7.9, 8.9, 10.3, 12.6, 13.3, 14.4, 15.2, 16.5, 
                 156.98 
               
               
                   
                 A1 
                 17.0, 17.7, 18.3, 19.5, 20.5 
               
               
                 Form 1 
                 WO2013080217 
                 7.3, 8.1, 8.9, 9.8, 10.4, 11.1, 12.7, 14.3, 15.3, 
                 134.01, 
               
               
                   
                 A2 
                 15.8 
                 159.58 
               
               
                 Form 2 
                 WO2013080217 
                 3.9, 6.9, 7.8, 10.2, 10.7, 11.6, 12.2, 12.8, 13.6, 
                 68.5, 
               
               
                   
                 A2 
                 14.0, 15.1, 17.2, 18.1 
                 114.9, 
               
               
                   
                   
                   
                 174 
               
               
                 Form 3 
                 WO2013080217 
                 4.2, 6.9, 7.5, 8.5, 8.6, 10.1. 11.0, 11.8, 12.3, 
                 71.59 
               
               
                   
                 A2 
                 12.6, 13.3, 13.4, 13.8, 14.3, 35.1, 15.6 
               
               
                 Form 4 
                 WO2013080217 
                 6.9, 7.9, 10.2, 10.7, 12.2, 13.9, 15.1, 17.2, 
                 45.35, 
               
               
                   
                 A2 
                 18.1, 19.8 
                 124.92 
               
               
                 Form 5 
                 WO2013080217 
                 7.5, 7.9, 8.6, 10.0, 12.6, 13.3, 14.1. 14.8, 15.0, 
                 56.29, 
               
               
                   
                 A2 
                 15.8, 16.7, 17.4, 18.0, 18.8, 19.4, 20.1 
                 145.27 
               
               
                 Form 6 
                 WO2013080217 
                 7.5, 7.9, 8.6, 9.0, 10.0, 12.5, 13.2, 13.8, 14.1, 
                 157.26 
               
               
                   
                 A2 
                 15.0, 15.5, 15.8, 16.6, 17.3, 17.8, 18.8, 19.6, 
               
               
                   
                   
                 20.1 
               
               
                 Form 7 
                 WO2013080217 
                 5.2, 6.0, 7.5, 8.9, 9.5, 10.1, 10.7, 11.7, 12.1, 
                 162 
               
               
                   
                 A2 
                 12.8, 13.3, 14.1, 15.3, 16.1, 17.1, 17.6, 18.1, 
               
               
                   
                   
                 18.9, 19.6 
               
               
                 Form 8 
                 W02013080217 
                 7.5, 7.9, 8.6, 10.0, 12.6, 13.3, 14.1, 14.8, 15.8, 
                 151.84, 
               
               
                   
                 A2 
                 16.7, 17.0, 17.5, 18.0, 18.9, 19.4 
                 159.08 
               
               
                 Form 9 
                 WO2013080217 
                 7.5, 7.9, 15.0, 15.8, 18.1, 19.4, 20.1, 22.6 
                 156.51 
               
               
                   
                 A2 
               
               
                 Form 10 
                 WO2013080217 
                 7.4, 7.7, 8.8, 10.1, 12.2, 12.7, 13.2, 14.4, 15.3, 
                 163.24 
               
               
                   
                 A2 
                 16.2, 16.9, 17.6, 18.0, 18.7, 19.4 
               
               
                 Form 11 
                 WO2013080217 
                 7.4, 7.7, 8.8, 10.1, 12.2, 12.7, 13.2, 14.4, 14.8, 
                 162.26 
               
               
                   
                 A2 
                 15.3, 15.6, 16.2, 16.9, 17.6, 18.0, 18.4, 19.4 
               
               
                 Form 12 
                 WO2013080217 
                 7.2, 7.7, 8.1, 8.9, 9.8, 10.3, 11.0, 12.6, 13.2, 
                 150.47 
               
               
                   
                 A2 
                 14.2, 14.8, 15.1, 15.7, 16.3, 17.1, 17.5, 18.5, 
               
               
                   
                   
                 19.4, 19.8 
               
               
                 Form 13 
                 WO2013080217 
                 7.6, 8.2, 8.9, 9.7, 10.2, 10.9, 11.7, 12.6, 13.1, 
                 119.57 
               
               
                   
                   
                 13.6, 14.5, 15.1, 15.8, 16.2, 17.0, 17.8, 18.3, 
               
               
                   
                   
                 19.2, 19.9 
               
               
                 Ethyl acetate 
                 WO2013088335 
                 7.5, 7.9, 8.7, 10.1, 10.2, 12.6, 12.9, 13.8, 
                 N/A 
               
               
                 solvate 
                 A1 
                 14.1, 14.8 
               
               
                 Form W 
                 Present invention 
                 4.4, 7.8, 8.5, 11.4, 12.8, 15.3, 17.0, 20.4, 21.4 
                 153.78 
               
               
                   
                   
                 22.5, 23.4, 27.8, 29.7, 29.9, 33.3, 34.3