Abstract:
The present invention relates to the development of genetically engineered yeasts that can produce hydrocarbons in a controllable and economic fashion. More specifically the invention relates to the production of liquid alkanes and alkenes that can be used for liquid transportation fuels, specialty chemicals, or feed stock for further chemical conversion.

Description:
STATEMENT OF PRIORITY 
     This application is a 35 U.S.C. §371 national phase application of International Application Ser. No. PCT/SE2014/051229, filed Oct. 17, 2014, which claims the benefit, under 35 U.S.C. §119 (a) of United States Application Serial No. 61/893,125 filed Oct. 18, 2013, the entire contents of each of which are incorporated by reference herein. 
    
    
     STATEMENT REGARDING ELECTRONIC FILING OF A SEQUENCE LISTING 
     A Sequence Listing in ASCII text format, submitted under 37 C.F.R. §1.821, entitled 9737- 41 ST25.txt, 127,549 bytes in size, generated Apr. 14, 2016and filed via EFS-Web, is provided in lieu of a paper copy. This Sequence Listing is hereby incorporated herein by reference into the specification for its disclosures. 
     FIELD OF THE INVENTION 
     The present invention relates to the development of genetically engineered yeasts that can produce hydrocarbons in a controllable and economic fashion. More specifically the invention relates to the production of, for instance liquid alkanes and alkenes, that can be used for liquid transportation fuels, specialty chemicals, or feed stock for further chemical conversion. 
     DESCRIPTION OF THE RELATED ART 
     Increased petroleum prices along with concerns about carbon dioxide emission and the lack of sustainability of fossil fuels have been strongly motivating the development and production of biofuels. As about 80% of mineral oils are being used for liquid transportation fuels, there is particular focus on developing alternative biotech processes to replace these. 
     Currently, the dominating biofuel is ethanol. This is produced in very large quantities, particularly in Brazil from sugar cane and in the USA from corn, but there are also several key initiatives on establishing so-called second-generation bioethanol production, where cellulosic biomass is used as the feedstock. The production of advanced biofuels to be used as gasoline does not solve a major problem associated with ensuring provision of jetfuels and fuels for maritime and heavy duty road transportation, both of which require high-density fuels—generally known as diesel-fuels. 
     Currently, biodiesel is produced from vegetable oils, but this biodiesel production is problematic since it competes against use of these oils in the food sector. Furthermore, the yield of oil per hectare is very low compared with that of sugar cane or other sugar crops. This type of biodiesel consists mainly of fatty acid alkyl esters (FAAEs). Recently, initiatives have been started to produce FAAEs in microorganisms such as the bacterium  Escherichia coli  and the yeast  Saccharomyces cerevisiae  with sugars as substrate, which would allow for higher per hectare yields resulting in a lower environmental impact. A disadvantage of FAAEs is that they contain oxygen, which leads to a lower energy density compared to pure hydrocarbon molecules. 
     SUMMARY OF THE INVENTION 
     A primary object of the present invention is to provide a genetically engineered yeast that can produce hydrocarbons, including but not limited to alkanes and alkenes, in a controllable and economic fashion. 
     An aspect of the embodiments relates to a yeast lacking a gene encoding hexadecanal dehydrogenase (HFD1) or comprising a disrupted gene encoding HFD1. The yeast also comprises at least one heterologous gene encoding an enzyme involved in a pathway of producing hydrocarbons. 
     Another aspect of the embodiments relates to a method for producing hydrocarbons. The method comprises culturing a yeast lacking a gene encoding hexadecenal dehydrogenase (HFD1) or comprising a disrupted gene encoding HFD1 in culture conditions suitable for production of the hydrocarbons from the yeast. The method also comprises collecting the hydrocarbons from the culture medium in which the yeast is cultured and/or from the yeast. 
     A further aspect of the embodiments relates to use of a yeast lacking a gene encoding hexadecenal dehydrogenase (HFD1) or comprising a disrupted gene encoding HFD1 for the production of hydrocarbons. In one embodiment,  Saccharomyces cerevisiae  was metabolically engineered to synthesize medium-chain alkanes. The inventors identified and demonstrated the importance of eliminating hexadecenal dehydrogenase Hfd1 in combination with heterologous expression of one or more enzymes, and/or biosynthetic and/or metabolic pathways, in enabling biosynthesis of the former compounds in yeast. The requirement of HFD1 deletion further illustrates a key difference between yeast and bacteria, in which the main competing enzymes are fatty aldehyde reductases and fatty alcohol dehydrogenases that convert the fatty aldehyde intermediate reversibly into a fatty alcohol. 
     The fatty acid derivatives (e.g., alkanes, alkenes, fatty alcohols) produced by the recombinant yeast of this invention are liquid (e.g., carbon chains with 5-17 carbon atoms). Such liquid alkanes and/or alkenes can be used, for example, as liquid transportation fuels. 
     These and other aspects of the invention are set forth in more detail in the description of the invention below. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  shows the alkane biosynthetic pathway and fatty aldehyde metabolism in  Saccharomyces cerevisiae . A heterologous alkane biosynthetic pathway, consisting of a  S. elongatus  fatty acyl-CoA/ACP reductase (SeFAR; encoded by orf1594) and a  S. elongatus  fatty aldehyde deformylating oxygenase (SeFADO; encoded by orf1593), was introduced in the yeast  S. cerevisiae . This pathway intersects with endogenous metabolism of fatty aldehydes by promiscuous aldehyde reductase (ALR) and fatty alcohol dehydrogenases (ADH) and the hexadecenal dehydrogenase Hfd1 (encoded by HFD1/YMR110C), which catalyzes the last step in the sphingolipid breakdown pathway. The  E. coli  ferredoxin (EcFdx)/ferredoxin reductase (EcFpr) system was introduced to provide the cofactor required for the FADO enzyme. The endogenous ferredoxin and ferredoxin reductase homologues Yah1 and Arh1, respectively, are localized to the mitochondria. 
         FIG. 2  shows analysis of alkane (A) and fatty alcohol production (B) in engineered  S. cerevisiae  strains. Strains carry either the WT allele or a deletion of the HFD1 gene encoding hexadecenal dehydrogenase and express  S. elongatus  fatty acyl-CoA/ACP reductase (FAR),  S. elongatus  fatty aldehyde deformylating oxygenase (FADO), and/or  E. coli  ferredoxin/ferredoxin reductase (F/FNR). The error bars represent the standard deviation of three biological replicates. 
         FIG. 3  shows alkane biosynthesis. Gas chromatograms of shake flask cultures incubated for 48 hours in glucose minimal medium. The lines represent  S. cerevisiae  CEN.PK113-11C strains that express  S. elongatus  FAR and FADO as well as the  E. coli  reduction system consisting of Fdx (F) and Fpr (FNR). The  S. cerevisiae  strains carrying an empty vector pYX212 (black and brown traces) are shown as a control. A C7-C30 alkane analytical standard (purple trace) was used as a reference. The peaks highlighted by the blue bars labeled with I, II, IS, and III represent tridecane (C13), pentadecane (C15), hexadecane (C16; internal standard), and heptadecane (C17), respectively. The shown spectra are for the m/z values 184, 212, and 240. The mass spectra for the labeled peaks in comparison with a NIST library standard. 
         FIG. 4  shows gas chromatography spectrum of intracellular alkanes and alkenes produced in a  Saccharomyces cerevisiae  BY4741 wt strain carrying the plasmids KB02 and pAlkane0 (WT S/S/F/F 2 I) and the strain BY4741 6550 (hfd1Δstrain, S/S/F/F 4 III) carrying the same plasmids. The four dashed peaks represent the alkane standard that was analyzed under the same conditions; the peak at 17.6 minutes is a pentadecane peak. This spectrum illustrates the requirement of the HFD1 deletion for fatty acid derivatives produced via a fatty aldehyde intermediate pathway. 
         FIG. 5  shows gas chromatography spectrum of intracellular alkanes and alkenes produced in a  Saccharomyces cerevisiae  BY4741 6550 strain carrying the plasmids KB02 and pAlkane0 (S/S/F/F 1 III) and the control strain BY4741 6550 (hfd1Δstrain) carrying the empty plasmids pIYC04 and pSPGM1 (control). The five dashed peaks represent the alkane standard that was analyzed under the same conditions; the peak at 17.6 and 20.1 minutes are a pentadecane and a heptadecane peak, the peak (4th) after 21 minutes represents the internal standard 1-octadecene. This spectrum illustrates that introduction of a cyanobacterial alka/ene biosynthesis pathway and deletion of HFD1 enables yeast to produce hydrocarbons. 
         FIG. 6  describes direct repeat-mediated marker removal. 
         FIG. 7  describes the pathway for the biosynthesis of free fatty acids in yeast cells from cytosolic acetyl-CoA that may result from overexpression of the specified bacterial or yeast genes in the cytosol of a yeast cell. 
         FIG. 8  shows the change in produced fatty alcohol profile when a fatty-acid producing pathway composed of an acetyl-CoA C-acetyltransferase, a 3-ketoacyl-CoA thiolase, a 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase multifunctional enzyme, a trans-enoyl-CoA reductase and a thioesterase is overexpressed in  S. cerevisiae  cytoplasm. 
         FIG. 9  describes constructs for integration into  S. cerevisiae  strain CEN.PK 113-11C for cytosolic overexpression of the medium-chain fatty acid biosynthesis pathway. 
         FIG. 10  shows overexpression of  Rhodosporidium toruloides  ACC1, FAS1+FAS2, and ACC1+FAS1+FAS2 in a storage lipid free  Saccharomyces cerevisiae . Cells were cultivated and total lipids were measured as described by Khoomrung et al (2012). 
         FIG. 11  shows an alkane sensor system, where ARE binding sites were fused to a minimal TEF promoter; by expressing the different components of the sensor system a dynamical range of 100-fold was achieved. 
         FIGS. 12A-12B  show an overview of cytosolic and mitochondrial fatty acid biosynthesis and alkane, alkene, and fatty alcohol biosynthesis using fatty acid derivatives as substrate. Note: cytosolic acyl-CoA does not exist in this form during the fatty acid biosynthetic process but is released as such upon termination of it. 
         FIG. 13  shows the DNA pathway assembly constructs used to construct pAlkane1, pAlkane7, pAlkane8, and pFAR.  Synechoccocus elongatus  fatty acyl-ACP/CoA reductase (SeFar) and  S. elongatus  fatty aldehyde deformylating oxygenase (SeFad) were synthesized and codon-optimized.  Escherichia coli  ferredoxin (EcFdx) and  E. coli  ferredoxin NADP+ reductase (SeFpr) were amplified from  E. coli  DH5α. The promoter pTPI and the terminator tpYX212 are homologous to the respective promoter and terminator on the pYX212 plasmid. All four plasmids were constructed using the modular pathway engineering strategy (Zhou et al., 2012). 
         FIG. 14  shows alkane biosynthesis. Gas chromatograms of shake flask cultures incubated for 48 hours in glucose minimal medium. The lines represent  S. cerevisiae  CEN.PK113-11C strain carrying deletion of the HFD1 gene and which express  Photorhabdus luminescens  LuxC, LuxD, and LuxE; and either a  S. elongatus  or a  N. punctiforme  FADO. The  S. cerevisiae  strains carrying an empty vector pYX212 (bottom trace) are shown as a control. A C7-C30 alkane analytical standard (top trace) was used as a reference. The peaks highlighted by the blue bars labeled with I, II, IS, and III represent tridecane (C13), pentadecane (C15), hexadecane (C16; internal standard), and heptadecane (C17), respectively. The shown spectra are for the m/z values 184, 212, and 240. The mass spectra for the labeled peaks in comparison with a NIST library standard. 
         FIG. 15  shows the DNA pathway assembly constructs used to construct pAlkane1, pAlkane7, and pAlkane8.  Synechoccocus elongatus  fatty acyl-ACP/CoA reductase (SeFar) and  S. elongatus  fatty aldehyde deformylating oxygenase (SeFad) were synthesized and codon-optimized.  Escherichia coli  ferredoxin (EcFdx) and  E. coli  ferredoxin NADP+ reductase (SeFpr) were amplified from  E. coli  DH5α. The promoter pTPI and the terminator tpYX212 are homologous to the respective promoter and terminator on the pYX212 plasmid. All four plasmids were constructed using the modular pathway engineering strategy (Zhou et al., 2012). 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The present invention now will be described hereinafter with reference to the accompanying drawings and examples, in which embodiments of the invention are shown. This description is not intended to be a detailed catalog of all the different ways in which the invention may be implemented, or all the features that may be added to the instant invention. For example, features illustrated with respect to one embodiment may be incorporated into other embodiments, and features illustrated with respect to a particular embodiment may be deleted from that embodiment. Thus, the invention contemplates that in some embodiments of the invention, any feature or combination of features set forth herein can be excluded or omitted. In addition, numerous variations and additions to the various embodiments suggested herein will be apparent to those skilled in the art in light of the instant disclosure, which do not depart from the instant invention. Hence, the following descriptions are intended to illustrate some particular embodiments of the invention, and not to exhaustively specify all permutations, combinations and variations thereof. 
     Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. 
     All publications, patent applications, patents and other references cited herein are incorporated by reference in their entireties for the teachings relevant to the sentence and/or paragraph in which the reference is presented. 
     Unless the context indicates otherwise, it is specifically intended that the various features of the invention described herein can be used in any combination. Moreover, the present invention also contemplates that in some embodiments of the invention, any feature or combination of features set forth herein can be excluded or omitted. To illustrate, if the specification states that a composition comprises components A, B and C, it is specifically intended that any of A, B or C, or a combination thereof, can be omitted and disclaimed singularly or in any combination. 
     An alternative type of diesel fuel can include terpene derived hydrocarbons. Since terpene derived diesels need chemical finishing due to the unsaturated nature of the primary fermentation products, an ideal biofuel would comprise saturated alkanes, which are also the main component of petrodiesels. Biosynthetically they are derived from fatty acids, which are constructed from the building block acetyl-CoA. 
     Biosynthetic pathways leading to alkane formation have however only been elucidated very recently, mainly in plants and bacteria. The“n-1 pathway” from cyanobacteria, is a two-step process, in which activated fatty acids are first reduced to fatty aldehydes and then decarbonylated to form alkanes. This pathway was transferred to  E. coli  and the resultant recombinant  bacterium  has been used in a fermentation process developed by a US-based company LS9. 
     The present invention provides a further industrial organism, yeast, that produces fatty acid derivatives (e.g., alkanes, alkenes, fatty alcohols and the like). Using today&#39;s methods, production of such fatty acid derivatives has not been efficient in yeast, since the yields are too low and it has not been possible to obtain short/medium chain fatty acid derivatives. However, the present inventors surprisingly discovered that deleting a hexadecenal dehydrogenase gene, HFD1, in yeast led to the blocking of the conversion of fatty aldehyde to fatty acid, thereby resulting in the production of, for example, alkanes, alkenes and fatty alcohols. Due to its adaptability to fermentation conditions, such as low pH, yeast provides an ideal industrial microorganism for the production of these fatty acid derivatives. 
     The HDF1 gene in yeast has so far only been studied in the context of Sjögren-Larssons disease, but has never been associated with production of fatty acid derivatives as in the present invention. Hexadecenal dehydrogenase Hfd1 (encoded by HFD1) competes for substrate with the heterologous fatty aldehyde decarbonylases leading to an ATP consuming futile cycle. By the discovery of the present inventors that a knock-out of this gene in yeast, alone or in combination with the integration of one or more heterologous nucleotide sequences and/or biosynthetic pathways, can alter the products of fatty acid biosynthesis and metabolism, the inventors have provided a solution to the utilization of different fatty acid biosynthetic machineries in the cytosol and in the mitochondria, respectively for the synthesis of medium and long chain fatty acids, and their subsequent conversion into alkanes, alkenes and/or fatty alcohols. 
     In the present invention, the inventors demonstrate fatty acid derived alkane biosynthesis in the yeast  S. cerevisiae  by expression of an alkane biosynthetic pathway consisting of a FAR, encoded by  Synechoccocus elongatus  orf1594, and a FADO, encoded by  S. elongatus  orf1593 (see  FIG. 1 ). However, upon first instance of expression of the SeFAR and SeFADO in a  S. cerevisiae  CEN.PK background, no alkanes could be detected ( FIG. 2A , KB 16). The inventors suspected that an explanation for the absence of alkanes could be the lack of a compatible redox partner that is required by the FADO enzyme in the CEN.PK background strain. For the FADO enzyme it has been shown in vitro that it requires ferredoxin (F) and ferredoxin NADP+ reductase (FNR) to supply electrons. Yeast possesses the ferredoxin and the ferredoxin reductase homologs Yah1 and Arh1, respectively, which both play a role in iron-sulfur cluster protein biosynthesis. Nonetheless, these proteins reside in the mitochondria, which makes them inaccessible as redox partners for the cytosolic alkane pathway. Since  E. coli  was able to support in vivo alkane production, we chose to co-express the  E. coli  ferredoxin (F) Fdx and ferredoxin NADP+ reductase (FNR) Fpr. The co-expression of the EcF/FNR reducing system resulted in the biosynthesis of 2.7±0.9 mg/gDW heptadecane ( FIG. 2A , KB 17) and no detection of pentadecane. This result is in contrast with the alkane profile that was found in  E. coli  as well as the fatty acid profile of  S. cerevisiae , in which C16 and C18 are the predominant fatty acid species. The inventors speculated that there might be a problem with supplying C16 fatty aldehydes for the decarbonylation reaction. 
     Hence, to ensure efficient functionality of the pathway the inventors chose to verify the fatty aldehyde supply by FAR. To confirm the supply of fatty aldehydes, fatty alcohol synthesis was used as an indicator. The detection of fatty alcohols as byproducts of alkane biosynthesis has been observed in  E. coli , and is suspected to be a result of the activity of endogenous promiscuous aldehyde reductases and alcohol dehydrogenases. Yeast contains around 40 homologues of such reductases and dehydrogenases, and consequently fatty alcohol synthesis was expected to occur after the introduction of FAR. Nevertheless, when SeFAR was overexpressed in a wild-type yeast strain, it yielded only trace amounts of fatty alcohols ( FIG. 2B ). These results indicated that there could be an additional (irreversible) reaction, not present in  E. coli , which competes for the fatty aldehyde substrate. In the case of  S. elongatus  it has recently been shown that such an enzyme is present and that overexpression of FAR results in fatty acid secretion due to the presence of the fatty aldehyde dehydrogenase AldE. This enzyme converts fatty aldehydes very efficiently into fatty acids. Alignment of AldE against the  S. cerevisiae  proteome yielded the hexadecenal dehydrogenase Hfd1 as the primary candidate. To test the hypothesis that Hfd1 prevents the biosynthesis of fatty alcohols by converting fatty aldehydes into fatty acids, HFD1 was knocked-out followed by SeFAR overexpression. Surprisingly, the deletion of HFD1 alone sufficed to enable fatty alcohol production (1.5±0.1 mg/L,  FIG. 2B ). The fatty aldehydes observed in this hfd1Δ strain most likely resulted from the sphingolipid breakdown pathway in which Hfd1 catalyzes the final step. The additional overexpression of SeFAR increased the fatty alcohol titer to 1.8±0.1 mg/L. The main fatty alcohol was hexadecanol (C16:0; 79%), followed by tetradecanol (C14:0; 11%), hexadecenol (C16:1; 7.3%), and dodecanol (C12:0; 2.8%). The drastic increase of C16 fatty alcohols illustrated that Hfd1 catalyzed the oxidation of C16 fatty aldehydes toward the corresponding fatty acids. 
     The detection of heptadecane in the wild-type background strain KB 17 carrying SeFAR, SeFADO, and EcF/FNR and the absence of the fatty alcohol octadecanol in the hfd1Δ SeFAR strain suggests that Hfd1 and the endogenous aldehyde reductases/alcohol dehydrogenases cannot use octadecanal as a substrate. This is in agreement with the detection of very long chain alkanes. The modest increase in fatty alcohol titer after FAR expression in a hfd1Δ strain, is most likely due to the low affinity of FAR for fatty acyl-CoA (it prefers fatty acyl-ACP). These results illustrate the importance of HFD1 deletion to enable fatty aldehyde supply. 
     Subsequently, the SeFADO and the EcF/FNR reducing system were introduced in the hfd1Δ strain, as deletion of HFD1 alone is sufficient to provide fatty aldehydes for the upstream part of the alkane pathway (which had been shown by the increased production of fatty alcohols). Subsequently, the alkane production increased drastically to 18.6±1.4 mg/gDW in this hfd1Δ SeFADO EcF/FNR strain ( FIG. 2A , KB 18). Accumulation of tridecane and pentadecane was observed together with heptadecane, which was the sole product in the wild-type genetic background strain KB 17. The chain length profile of these alkanes is in agreement with those of the observed fatty alcohols. Additional expression of SeFAR in the hfd1Δ strain resulted in a titer of 22.0±1.4 mg/gDW. The slight increase in titer suggests again that SeFAR has low catalytic efficiency on acyl-CoAs. No alkanes were detected extracellularly indicating that the alkanes are not excreted, which is in contrast with the detection of 80% of the produced alkanes in the extracellular medium in  E. coli.    
     Similarly, we also realized medium-chain alkane production after HFD1 disruption in a S288C background. Interestingly, expression of only SeFAR and SeFADO in this strain resulted in pentadecane and heptadecane biosynthesis, possibly indicating the presence of a reducing system that is absent in the CEN.PK background strain. 
     In some embodiments of the invention, yeasts can be modified to overproduce acyl-CoA, fatty acids or acyl-ACPs in order to further increase the production of alkanes, alkenes and/or fatty alcohols. In one embodiment, increasing the fatty acid synthesis can be accomplished by overexpressing fatty acid biosynthetic genes, including but not limited to ACC1 (encoding acetyl-CoA carboxylase), FAS1 (encoding the beta-subunit of fatty acid synthetase) and FAS2 (encoding the alpha-subunit of fatty acid synthetase). The inventors have in addition to these pathways also expressed several alternative alkane/alkene biosynthetic pathways in order to enable the biosynthesis of short, medium, and long chain alkanes, alkenes, and the like. 
     In some aspects of the invention, alkenes and/or alkanes with 5-17 carbon atoms are preferred. To achieve these chain lengths, the chain length of the fatty-acids used for conversion to alkanes and/or alkenes can be regulated. 
     For example, shorter chain molecules can be obtained by introducing a fatty acid synthase from humans, by expression of the alkane/alkene pathways in the mitochondria, or by reversed beta-oxidation in the cytosol. 
     A still further aspect comprises eliminating non-essential pathways in the yeast that consume (activated) fatty acids and thus compete with the production of fatty acid derivatives. Such nonessential pathways can include but are not limited to elimination of storage lipid formation and peroxisomal beta-oxidation. 
     In an additional embodiment, the NADPH supply can be modified (e.g., increased) in the recombinant yeast. Since NADPH is an essential cofactor of fatty acid biosynthesis, by increasing the supply of NADPH, it may be possible to further increase the production of fatty acid derivatives according to this invention. 
     Hence, in one embodiment, the invention provides a genetically modified/non-native strain of yeast comprising a disrupted gene encoding hexadecenal dehydrogenase (HFD1). 
     In some aspects of the invention, the disruption of the HFD1 gene results in a gene that is inoperative or knocked out and/or a nonfunctional gene product (e.g., a polypeptide having no activity as compared to the activity of the Hfd1 wild type polypeptide). In other embodiments, the disruption of the HFD1 gene results in a gene product that has reduced activity (e.g., 0 to 20% of the activity of the HFD1 wild type polypeptide). In still other embodiments, the disruption of the HFD1 gene results in reduced expression of a gene product as compared to the Hfd1 wild type polypeptide. 
     As used herein the terms a ype polypepHFD1 geneed herein the terms a ype polypepHFD1″ are used interchangeably. 
     A “disrupted gene” as defined herein involves any mutation or modification to a gene resulting in a partial or fully non-functional gene and gene product. Such a mutation or modification includes, but is not limited to, a missense mutation, a nonsense mutation, a deletion, a substitution, an insertion, and the like. Furthermore, a disruption of a gene can be achieved also, or alternatively, by mutation or modification of control elements controlling the transcription of the gene, such as mutation or modification in a promoter and/or enhancement elements. In such a case, such a mutation or modification results in partially or fully loss of transcription of the gene, i.e. a lower or reduced transcription as compared to native and non-modified control elements. As a result a reduced, if any, amount of the gene product will be available following transcription and translation. 
     The objective of gene disruption is to reduce the available amount of the gene product, including fully preventing any production of the gene product, or to express a gene product that lacks or having lower enzymatic activity as compared to the native or wild type gene product. 
     A yeast useful with this invention can be any yeast useful in industrial and fermentation practices. In one embodiment, the yeast can be from the genus  Saccharomyces.  In other embodiments, the yeast is  Saccharomyces cerevisiae.    
     In some embodiments, the genetically modified yeast strain of this invention (e.g., comprising at least a disrupted HDF1 gene) can further comprise one or more additional genetic modifications to improve production of desired products. Such modifications can include, but are not limited to: 
     (1) introduction of new enzymes, and/or biosynthetic and/or metabolic pathways, including, but not limited to expression of an alkane biosynthetic pathway consisting of  Synechoccous elongatus  FAR and  Synechoccous elongatus  FADO and; 
     (2) optionally, ferrodoxin (F) and ferrodoxin NADP+ reductase (FNR) may be introduced to supply electrons. 
     In still some embodiments, the yeast strains of the invention can additionally comprise genetic modifications that eliminate or reduce non-essential pathways. Such modifications can eliminate or reduce the utilization or consumption of fatty acids by enzymes or pathways that compete with the production of fatty acid derivatives such as alkanes, alkenes and fatty alcohols in the recombinant yeast strains. Exemplary embodiments of such non-essential pathways can include but are not limited to storage lipid formation and beta-oxidation. In particular embodiments, storage lipid formation can be eliminated or reduced by disrupting the genes encoding, for example, acyl-CoA:sterol acyltransferase (ARE1, ARE2), diacylglycerol acyltransferase (DGA1, LRO1). In other embodiments, beta-oxidation and free fatty acid activation can be eliminated or reduced by disrupting the genes encoding, for example, PDX1, FAA1, FAA4. 
     In additional aspects of the invention, the genetically modified yeast of the invention can be further modified to express heterologous fatty acid biosynthetic polypeptides for increased production of fatty acids. Nonlimiting examples of genes encoding such heterologous polypeptides Acc1, Fas1 and Fas2 (from e.g.,  Rhodosporidium toruloides ). 
     NADPH is a cofactor in the synthesis of fatty acids. To increase the availability of NADPH for fatty acid biosynthesis, the genetically modified yeast of the invention can be further modified for heterologous expression of non-phosphorylating NADP+-dependent glyceraldehydes-3-phosphate dehydrogenase (GAPN) (from e.g.,  Streptococcus mutans ). In other aspects, the yeast can be modified to disrupt GDH1 encoding NADP-dependent glutamate dehydrogenase. In still other embodiments, the yeast of the invention can be further modified to overexpress GDH2 encoding NAD-dependent glutamate dehydrogenase. 
     In additional embodiments, the yeast of the invention (e.g., comprising at least a disrupted HDF1 gene) can be further modified to comprise genetic modifications to increase production of fatty acid derivatives having particular chain lengths (e.g., short, medium, long chain fatty acid derivatives). In one aspect, the yeast can be modified to express a chimeric cytosolic pathway for the production of medium chain fatty acids or an increased ratio of medium-chain to long-chain fatty acids. Thus, for example, the yeast can be modified to (over)express in the cytosol FOX2, FOX3, ERG10 and TES1 (derived from, for example,  S. cerevisiae ), and/or yqeF, fadA, fabB and tdTER (from bacteria). 
     Fatty acid chain length can also be regulated through modification of expression of thioesterases. Thus, in some embodiments, the yeast of this invention can be further modified to express a thiesterase having a desired chain length specificity (e.g., tesA, tesB, fadM, yciA from, e.g.  E. coli ). 
     In particular embodiments, the genetically modified yeast of the invention can be further modified to produce short chain fatty acid derivatives (e.g., alkanes, alkenes, fatty alcohols and the like). Non-limiting examples of genes useful for such modifications include fpr and fdx from, for example,  E. coli;  and/or ferredoxin (orf_1499, petF) and ferredoxin-NADPH reductase (orf_0978, petH) from  Synechococcus elongatus . Accordingly, in some embodiments, a genetically modified yeast of this invention can further comprise nucleic acid constructs comprising nucleotide sequences encoding fpr and/or fdx and/or nucleotide sequences encoding petF and/or petH. 
     In additional embodiments, the yeast strains of this invention (e.g., comprising at least a disrupted HFD1 gene) can further comprise nucleic acid constructs comprising nucleotide sequences encoding enzymes and/or biosynthetic pathways for conversion of fatty acids to alkanes and/or alkenes. Thus in some embodiments, the genetically modified yeast of the invention can be further modified to express  Mycobacterium marinum  carboxylic acid reductase and  Musca domestica  CYP4G2 decarbonylase (decarbonylase is also referred to as deformylating oxygenase in the art). In a representative embodiment, the yeast can be further modified to express a thioesterase, or an additional thioesterase, to relieve fatty acid biosynthess repression by acyl-CoA and to increase substrate availability for alkane and alkene biosynthesis. In other embodiments, the yeast strains of the invention can be modified to comprise expression of  Synechococcus elongatus  orf1594 and ACS,  Musca domestica  CYP4GT decarbonylase and NADPH-cytochrome P450 reductase. In further embodiments, the yeast strains of the invention can be modified to express  Acinetobacter baylyi  Acr1,  Musca domestica  CYP4GT decarbonylase and NADPH-cytochrome P450 reductase. 
     In further aspects of the invention, the bacterial luminescence pathway and a cyanobacterial fatty aldehyde decarbonylase can be expressed in the yeast strains of the invention in order to utilize fatty acyl-CoA in the synthesis of alkanes and alkenes. Thus, in a representative embodiment, the yeast strains of the invention comprising at least a disrupted HFD1 gene further comprises LuxC, LuxD and LuxE from  Photorhabdus luminescens  and  Nostoc punctiforme  FAD. 
     In other embodiments, the yeast strains of the invention can be further modified to comprise a pathway for conversion of fatty acids to terminal alkenes. A nonlimiting example of such a pathway includes  Jeotgalicoccus  spp orf880,  Escherichia coli  GroEL and  Escherichia coli  GroES. 
     The genetically modified yeast strain can additionally comprise carboxylic acid reductase (from e.g.,  Mycobacterium marinum ) and decarbonylase (from e.g.,  Musca domestica ) for conversion of fatty acids to alkanes and alkenes. 
     In some embodiments, short chain alkanes and alkenes are the desired product. Accordingly, in some embodiments, the genetically modified yeast of the invention can comprise modifications to their mitochondrial fatty acid biosynthetic pathway. In a representative embodiment, the genetically modified yeast of the invention can be modified to express in their mitochondria the  Mycobacterium marinum  CAR fatty acid reductase, the  Nostoc puntiforme  fatty aldehyde decarbonylase and  Aspergillus nidulans  phosphopantetheinyl transferase NpgA, optionally, the yeast can be modified to additionally overexpress components of the yeast mitochondrial fatty acid biosynthetic pathway, including but not limited to Etr1 (2-enoyl thioester reductase) and Hfa1 (acetyl-CoA carboxylase). In some embodiments, the yeast mitochondrial fatty acid biosynthetic pathway components to be overexpressed can further comprise CEM1, HTD2, OAR1, and MCT1. In further embodiments, the yeast comprising modifications to their mitochondrial fatty acid biosynthetic pathway can additionally comprise fdx and fpr from  E. coli , wherein the respective protein sequences comprise mitochondrial localization signal(s) to direct them to the mitochondria In still further embodiments, the yeast comprising modifications to their mitochondrial fatty acid biosynthetic pathway can additionally comprise nucleic acids encoding thioesterase to be expressed in the mitochondria. Non-limiting examples of thiesterases with activity towards medium chain fatty acyl-ACP include  Acinetobacter baylyi  TesA,  Cocos nucifera  FatB1, or homologue thioesterases thereof. 
     In additional embodiments, the yeast of the invention can be further modified to express a formate dehydrogenase enzyme in the mitochondria. Non-limiting examples of formate dehydrogenase enzymes include Fdh1 and/or Fdh2, which can be introduced into the yeast with mitochondrial localization signals. 
     In some embodiments the genetically modified yeast of the invention can be modified to have improved fatty aldehyde decarbonylase activity (thereby improving alkane and/or alkene production) by fusing a catalase to a fatty aldehyde decarbonylase (e.g.,  Synechoccocus elongatus  orf1593 or  Nostoc punctiforme  FAD). 
     In other embodiments, the genetically modified yeast strains of the invention can comprise  Yarrowia lipoytica  Yas3 repressor and a fluorescent protein expressed from an alkane response element, ARE 1 containing promoter in order to be able to screen genetically modified yeast strains, including, but not limited to, the yeast strains described in this invention, for modified alkane production (e.g., increased and/or reduced as compared to a control yeast strain not comprising said modification(s)). Thus, in some embodiments, a method of screening for modified production of alkanes comprises, introducing into a yeast strain of interest a  Yarrowia lipoytica  Yas3 repressor, the activators Yas1 and Yas2 and a fluorescent protein expressed from an alkane response element, ARE1, containing promoter, and detecting modified production of alkanes. 
     The present invention provides a further method of screening for modified production of alkanes and/or alkenes (e.g., increased and/or reduced as compared to a control yeast strain not comprising said modification(s)) based on the toxicity of fatty acid accumulation in yeast strains that are modified to have reduced or no storage lipid formation and/or beta-oxidation. Thus, the consumption of fatty acids by the introduced alkane biosynthetic pathways can be evaluated by monitoring the toxicity of the genetically modified yeast strains. 
     The present invention further provides methods for the production of hydrocarbons in genetically modified yeast, comprising culturing a genetically modified yeast of this invention and collecting the hydrocarbons. In some embodiments, a hydrocarbon can be a fatty acid derivative, for example, an alkane, an alkene, or a fatty alcohol. 
     Definitions 
     As used in the description of the invention and the appended claims, the singular forms “a,” “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. 
     Also as used herein, “and/or” refers to and encompasses any and all possible combinations of one or more of the associated listed items, as well as the lack of combinations when interpreted in the alternative (“or”). 
     The term “about,” as used herein when referring to a measurable value such as a dosage or time period and the like, is meant to encompass variations of ±20%, ±10%, ±5%, ±1%, ±0.5%, or even ±0.1% of the specified amount. 
     As used herein, phrases such as “between X and Y” and “between about X and Y” should be interpreted to include X and Y. As used herein, phrases such as “between about X and Y” mean “between about X and about Y” and phrases such as “from about X to Y” mean “from about X to about Y.” 
     The term “comprise,” “comprises” and “comprising” as used herein, specify the presence of the stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. 
     As used herein, the transitional phrase “consisting essentially of” means that the scope of a claim is to be interpreted to encompass the specified materials or steps recited in the claim and those that do not materially affect the basic and novel characteristic(s) of the claimed invention. Thus, the term “consisting essentially of” when used in a claim of this invention is not intended to be interpreted to be equivalent to “comprising.” 
     As used herein, the terms “increase,” “increases,” “increased,” “increasing,” and similar terms indicate an elevation of at least about 25%, 50%, 75%, 100%, 150%, 200%, 300%, 400%, 500% or more. 
     As used herein, the terms “reduce,” “reduces,” “reduced,” “reduction,” and similar terms mean a decrease of at least about 5%, 10%, 15%, 20%, 25%, 35%, 50%, 75%, 80%, 85%, 90%, 95%, 97% or more. In particular embodiments, the reduction results in no or essentially no (i.e., an insignificant amount, e.g., less than about 10% or even 5%) detectable activity or amount. 
     As used herein, the terms “express,” “expresses,” “expressed” or “expression,” and the like, with respect to a nucleic acid molecule and/or a nucleotide sequence (e.g., RNA or DNA) indicates that the nucleic acid molecule and/or a nucleotide sequence is transcribed and, optionally, translated. Thus, a nucleic acid molecule and/or a nucleotide sequence may express a polypeptide of interest or a functional untranslated RNA. A “functional” RNA includes any untranslated RNA that has a biological function in a cell, e.g., regulation of gene expression. Such functional RNAs include but are not limited to RNAi (e.g., siRNA, shRNA), miRNA, antisense RNA, anti-microRNA antisense oligodeoxyribonucleotide (AMO; see e.g., Lu et al. Nucleic Acids Res. 37(3):e24;: 10.1093/nar/gkn1053), ribozymes, RNA aptamers and the like. 
     As used herein, “overexpress,” “overexpressed,” “overexpression” and the like, in reference to a polynucleotide means that the expression level of said polynucleotide is greater than that for the same polynucleotide in its native or wild type genetic context (e.g., in the same position in the genome and/or associated with the native/endogenous regulatory sequences). A nucleotide sequence can be overexpressed by inserting it into an overexpression vector. Such vectors are known in the art. 
     A “heterologous” or a “recombinant” nucleotide sequence is a nucleotide sequence not naturally associated with a host cell into which it is introduced, including non-naturally occurring multiple copies of a naturally occurring nucleotide sequence. A heterologous gene may optionally be codon optimized for expression in yeast according to techniques well known in the art and as further described herein. A heterologous gene also encompasses, in some embodiments, an endogenous gene controlled by a heterologous promoter and/or control elements to achieve an expression of the gene that is different from, typically higher, i.e. so-called overexpression, than normal or baseline expression of the gene in a yeast comprising the endogenous gene under control of wild type (endogenous) promoter and control elements. 
     A “native” or “wild type” nucleic acid, nucleotide sequence, polypeptide or amino acid sequence refers to a naturally occurring or endogenous nucleic acid, nucleotide sequence, polypeptide or amino acid sequence. Thus, for example, a “wild type mRNA” is an mRNA that is naturally occurring in or endogenous to the organism. A “homologous” nucleic acid sequence is a nucleotide sequence naturally associated with a host cell into which it is introduced. 
     Also as used herein, the terms “nucleic acid,” “nucleic acid molecule,” “nucleotide sequence” and “polynucleotide” refer to RNA or DNA that is linear or branched, single or double stranded, or a hybrid thereof. The term also encompasses RNA/DNA hybrids. When dsRNA is produced synthetically, less common bases, such as inosine, 5-methylcytosine, 6-methyladenine, hypoxanthine and others can also be used for antisense, dsRNA, and ribozyme pairing. For example, polynucleotides that contain C-5 propyne analogues of uridine and cytidine have been shown to bind RNA with high affinity and to be potent antisense inhibitors of gene expression. Other modifications, such as modification to the phosphodiester backbone, or the 2′-hydroxy in the ribose sugar group of the RNA can also be made. 
     As used herein, the term “nucleotide sequence” refers to a heteropolymer of nucleotides or the sequence of these nucleotides from the 5′ to 3′ end of a nucleic acid molecule and includes DNA or RNA molecules, including cDNA, a DNA fragment or portion, genomic DNA, synthetic (e.g., chemically synthesized) DNA, plasmid DNA, mRNA, and anti-sense RNA, any of which can be single stranded or double stranded. The terms “nucleotide sequence” “nucleic acid,” “nucleic acid molecule,” “oligonucleotide” and “polynucleotide” are also used interchangeably herein to refer to a heteropolymer of nucleotides. Nucleic acid molecules and/or nucleotide sequences provided herein are presented herein in the 5′ to 3′ direction, from left to right and are represented using the standard code for representing the nucleotide characters as set forth in the U.S. sequence rules, 37 CFR §§1.821-1.825 and the World Intellectual Property Organization (WIPO) Standard ST.25. 
     As used herein, the term “gene” refers to a nucleic acid molecule capable of being used to produce mRNA, antisense RNA, miRNA, anti-microRNA antisense oligodeoxyribonucleotide (AMO) and the like. Genes may or may not be capable of being used to produce a functional protein or gene product. Genes can include both coding and non-coding regions (e.g., introns, regulatory elements, promoters, enhancers, termination sequences and/or 5′ and 3′ untranslated regions). A gene may be “isolated” by which is meant a nucleic acid that is substantially or essentially free from components normally found in association with the nucleic acid in its natural state. Such components include other cellular material, culture medium from recombinant production, and/or various chemicals used in chemically synthesizing the nucleic acid. 
     “Introducing” in the context of a yeast cell means contacting a nucleic acid molecule with the cell in such a manner that the nucleic acid molecule gains access to the interior of the cell. Accordingly, polynucleotides and/or nucleic acid molecules can be introduced yeast cells in a single transformation event, in separate transformation events. Thus, the term “transformation” as used herein refers to the introduction of a heterologous nucleic acid into a cell. Transformation of a yeast cell can be stable or transient. 
     “Transient transformation” in the context of a polynucleotide means that a polynucleotide is introduced into the cell and does not integrate into the genome of the cell. 
     By “stably introducing” or “stably introduced” in the context of a polynucleotide introduced into a cell, it is intended that the introduced polynucleotide is stably incorporated into the genome of the cell, and thus the cell is stably transformed with the polynucleotide. 
     “Stable transformation” or “stably transformed” as used herein means that a nucleic acid molecule is introduced into a cell and integrates into the genome of the cell. As such, the integrated nucleic acid molecule is capable of being inherited by the progeny thereof, more particularly, by the progeny of multiple successive generations. “Genome” as used herein includes the nuclear genome. Stable transformation as used herein can also refer to a nucleic acid molecule that is maintained extrachromasomally, for example, as a minichromosome. 
     Transient transformation may be detected by, for example, an enzyme-linked immunosorbent assay (ELISA) or Western blot, which can detect the presence of a peptide or polypeptide encoded by one or more nucleic acid molecules introduced into an organism. Stable transformation of a cell can be detected by, for example, a Southern blot hybridization assay of genomic DNA of the cell with nucleic acid sequences which specifically hybridize with a nucleotide sequence of a nucleic acid molecule introduced into an organism (e.g., a yeast). Stable transformation of a cell can be detected by, for example, a Northern blot hybridization assay of RNA of the cell with nucleic acid sequences which specifically hybridize with a nucleotide sequence of a nucleic acid molecule introduced into a yeast or other organism. Stable transformation of a cell can also be detected by, e.g., a polymerase chain reaction (PCR) or other amplification reaction as are well known in the art, employing specific primer sequences that hybridize with target sequence(s) of a nucleic acid molecule, resulting in amplification of the target sequence(s), which can be detected according to standard methods Transformation can also be detected by direct sequencing and/or hybridization protocols well known in the art. 
     The terms “complementary” or “complementarity,” as used herein, refer to the natural binding of polynucleotides under permissive salt and temperature conditions by base-pairing. For example, the sequence “A-G-T” binds to the complementary sequence “T-C-A.” Complementarity between two single-stranded molecules may be “partial,” in which only some of the nucleotides bind, or it may be complete when total complementarity exists between the single stranded molecules. The degree of complementarity between nucleic acid strands has significant effects on the efficiency and strength of hybridization between nucleic acid strands. 
     A “portion” or “fragment” of a nucleotide sequence of the invention will be understood to mean a nucleotide sequence of reduced length relative to a reference nucleic acid or nucleotide sequence and comprising, consisting essentially of and/or consisting of a nucleotide sequence of contiguous nucleotides identical or almost identical (e.g., 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 98%, 99% identical) to the reference nucleic acid or nucleotide sequence. Such a nucleic acid fragment or portion according to the invention may be, where appropriate, included in a larger polynucleotide of which it is a constituent. 
     Different nucleic acids or proteins having homology are referred to herein as “homologues.” The term homologue includes homologous sequences from the same and other species and orthologous sequences from the same and other species. “Homology” refers to the level of similarity between two or more nucleic acid and/or amino acid sequences in terms of percent of positional identity (i.e., sequence similarity or identity). Homology also refers to the concept of similar functional properties among different nucleic acids or proteins. Thus, the compositions and methods of the invention further comprise homologues to the nucleotide sequences and polypeptide sequences of this invention. “Orthologous,” as used herein, refers to homologous nucleotide sequences and/or amino acid sequences in different species that arose from a common ancestral gene during speciation. A homologue of a nucleotide sequence of this invention has a substantial sequence identity (e.g., at least about 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, and/or 100%) to said nucleotide sequence. 
     As used herein “sequence identity” refers to the extent to which two optimally aligned polynucleotide or peptide sequences are invariant throughout a window of alignment of components, e.g., nucleotides or amino acids. “Identity” can be readily calculated by known methods including, but not limited to, those described in:  Computational Molecular Biology  (Lesk, A. M., ed.) Oxford University Press, New York (1988);  Biocomputing: Informatics and Genome Projects  (Smith, D. W., ed.) Academic Press, New York (1993);  Computer Analysis of Sequence Data, Part I  (Griffin, A. M., and Griffin, H. G., eds.) Humana Press, New Jersey (1994);  Sequence Analysis in Molecular Biology  (von Heinje, G., ed.) Academic Press (1987); and  Sequence Analysis Primer  (Gribskov, M. and Devereux, J., eds.) Stockton Press, New York (1991). 
     As used herein, the term “percent sequence identity” or “percent identity” refers to the percentage of identical nucleotides in a linear polynucleotide sequence of a reference (“query”) polynucleotide molecule (or its complementary strand) as compared to a test (“subject”) polynucleotide molecule (or its complementary strand) when the two sequences are optimally aligned. In some embodiments, “percent identity” can refer to the percentage of identical amino acids in an amino acid sequence. 
     As used herein, the phrase “substantially identical,” in the context of two nucleic acid molecules, nucleotide sequences or protein sequences, refers to two or more sequences or subsequences that have at least about 70%, least about 75%, at least about 80%, least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% nucleotide or amino acid residue identity, when compared and aligned for maximum correspondence, as measured using one of the following sequence comparison algorithms or by visual inspection. In some embodiments of the invention, the substantial identity exists over a region of the sequences that is at least about 50 residues to about 150 residues in length. Thus, in some embodiments of the invention, the substantial identity exists over a region of the sequences that is at least about 16, at least about 18, at least about 22, at least about 25, at least about 30, at least about 40, at least about 50, about 60, about 70, about 80, about 90, about 100, about 110, about 120, about 130, about 140, about 150, or more residues in length, and any range therein. In representative embodiments, the sequences can be substantially identical over at least about 22 nucleotides. In still other embodiments, the substantial identiy exists over the full length or nearly the full length of the sequence. 
     For sequence comparison, typically one sequence acts as a reference sequence to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. 
     Optimal alignment of sequences for aligning a comparison window are well known to those skilled in the art and may be conducted by tools such as the local homology algorithm of Smith and Waterman, the homology alignment algorithm of Needleman and Wunsch, the search for similarity method of Pearson and Lipman, and optionally by computerized implementations of these algorithms such as GAP, BESTFIT, FASTA, and TFASTA available as part of the GCG® Wisconsin Package® (Accelrys Inc., San Diego, Calif.). An “identity fraction” for aligned segments of a test sequence and a reference sequence is the number of identical components which are shared by the two aligned sequences divided by the total number of components in the reference sequence segment, i.e., the entire reference sequence or a smaller defined part of the reference sequence. Percent sequence identity is represented as the identity fraction multiplied by 100. The comparison of one or more polynucleotide sequences may be to a full-length polynucleotide sequence or a portion thereof, or to a longer polynucleotide sequence. For purposes of this invention “percent identity” may also be determined using BLASTX version 2.0 for translated nucleotide sequences and BLASTN version 2.0 for polynucleotide sequences. 
     Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold. These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always &gt;0) and N (penalty score for mismatching residues; always &lt;0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when the cumulative alignment score falls off by the quantity X from its maximum achieved value, the cumulative score goes to zero or below due to the accumulation of one or more negative-scoring residue alignments, or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=−4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff &amp; Henikoff,  Proc. Natl. Acad. Sci. USA  89: 10915 (1989)). 
     In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin &amp; Altschul,  Proc. Nat&#39;l. Acad. Sci. USA  90: 5873-5787 (1993)). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a test nucleic acid sequence is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleotide sequence to the reference nucleotide sequence is less than about 0.1 to less than about 0.001. Thus, in some embodiments of the invention, the smallest sum probability in a comparison of the test nucleotide sequence to the reference nucleotide sequence is less than about 0.001. 
     Two nucleotide sequences can also be considered to be substantially identical when the two sequences hybridize to each other under stringent conditions. In some representative embodiments, two nucleotide sequences considered to be substantially identical hybridize to each other under highly stringent conditions. 
     “Stringent hybridization conditions” and “stringent hybridization wash conditions” in the context of nucleic acid hybridization experiments such as Southern and Northern hybridizations are sequence dependent, and are different under different environmental parameters. An extensive guide to the hybridization of nucleic acids is found in Tijssen  Laboratory Techniques in Biochemistry and Molecular Biology - Hybridization with Nucleic Acid Probes  part I chapter 2 “Overview of principles of hybridization and the strategy of nucleic acid probe assays” Elsevier, New York (1993). Generally, highly stringent hybridization and wash conditions are selected to be about 5° C. lower than the thermal melting point (T m ) for the specific sequence at a defined ionic strength and pH. 
     The T m  is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a perfectly matched probe. Very stringent conditions are selected to be equal to the T m  for a particular probe. An example of stringent hybridization conditions for hybridization of complementary nucleotide sequences which have more than 100 complementary residues on a filter in a Southern or northern blot is 50% formamide with 1 mg of heparin at 42° C., with the hybridization being carried out overnight. An example of highly stringent wash conditions is 0.1 5M NaCl at 72° C. for about 15 minutes. An example of stringent wash conditions is a 0.2×SSC wash at 65° C. for 15 minutes (see, Sambrook, infra, for a description of SSC buffer). Often, a high stringency wash is preceded by a low stringency wash to remove background probe signal. An example of a medium stringency wash for a duplex of, e.g., more than 100 nucleotides, is 1×SSC at 45° C. for 15 minutes. An example of a low stringency wash for a duplex of, e.g., more than 100 nucleotides, is 4-6×SSC at 40° C. for 15 minutes. For short probes (e.g., about 10 to 50 nucleotides), stringent conditions typically involve salt concentrations of less than about 1.0 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3, and the temperature is typically at least about 30° C. Stringent conditions can also be achieved with the addition of destabilizing agents such as formamide In general, a signal to noise ratio of 2× (or higher) than that observed for an unrelated probe in the particular hybridization assay indicates detection of a specific hybridization. Nucleotide sequences that do not hybridize to each other under stringent conditions are still substantially identical if the proteins that they encode are substantially identical. This can occur, for example, when a copy of a nucleotide sequence is created using the maximum codon degeneracy permitted by the genetic code. 
     The following are examples of sets of hybridization/wash conditions that may be used to clone homologous nucleotide sequences that are substantially identical to reference nucleotide sequences of the invention. In one embodiment, a reference nucleotide sequence hybridizes to the “test” nucleotide sequence in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 2×SSC, 0.1% SDS at 50° C. In another embodiment, the reference nucleotide sequence hybridizes to the “test” nucleotide sequence in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 1×SSC, 0.1% SDS at 50° C. or in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.5×SSC, 0.1% SDS at 50° C. In still further embodiments, the reference nucleotide sequence hybridizes to the “test” nucleotide sequence in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C., or in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 65° C. 
     In particular embodiments, a further indication that two nucleotide sequences or two polypeptide sequences are substantially identical can be that the protein encoded by the first nucleic acid is immunologically cross reactive with, or specifically binds to, the protein encoded by the second nucleic acid. Thus, in some embodiments, a polypeptide can be substantially identical to a second polypeptide, for example, where the two polypeptides differ only by conservative substitutions. 
     In some embodiments, the recombinant nucleic acids molecules, nucleotide sequences and polypeptides of the invention are “isolated.” An “isolated” nucleic acid molecule, an “isolated” nucleotide sequence or an “isolated” polypeptide is a nucleic acid molecule, nucleotide sequence or polypeptide that, by the hand of man, exists apart from its native environment and is therefore not a product of nature. An isolated nucleic acid molecule, nucleotide sequence or polypeptide may exist in a purified form that is at least partially separated from at least some of the other components of the naturally occurring organism or virus, for example, the cell or viral structural components or other polypeptides or nucleic acids commonly found associated with the polynucleotide. In representative embodiments, the isolated nucleic acid molecule, the isolated nucleotide sequence and/or the isolated polypeptide is at least about 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or more pure. 
     In other embodiments, an isolated nucleic acid molecule, nucleotide sequence or polypeptide may exist in a non-native environment such as, for example, a recombinant host cell. Thus, for example, with respect to nucleotide sequences, the term “isolated” means that it is separated from the chromosome and/or cell in which it naturally occurs. A polynucleotide is also isolated if it is separated from the chromosome and/or cell in which it naturally occurs in and is then inserted into a genetic context, a chromosome and/or a cell in which it does not naturally occur (e.g., a different host cell, different regulatory sequences, and/or different position in the genome than as found in nature). Accordingly, the recombinant nucleic acid molecules, nucleotide sequences and their encoded polypeptides are “isolated” in that, by the hand of man, they exist apart from their native environment and therefore are not products of nature, however, in some embodiments, they can be introduced into and exist in a recombinant host cell. 
     In some embodiments, the nucleotide sequences and/or recombinant nucleic acid molecules of the invention can be operatively associated with a variety of promoters for expression in yeast cells. Thus, in representative embodiments, a recombinant nucleic acid of this invention can further comprise one or more promoters operably linked to one or more nucleotide sequences. 
     By “operably linked” or “operably associated” as used herein, it is meant that the indicated elements are functionally related to each other, and are also generally physically related. Thus, the term “operably linked” or “operably associated” as used herein, refers to nucleotide sequences on a single nucleic acid molecule that are functionally associated. Thus, a first nucleotide sequence that is operably linked to a second nucleotide sequence, means a situation when the first nucleotide sequence is placed in a functional relationship with the second nucleotide sequence. For instance, a promoter is operably associated with a nucleotide sequence if the promoter effects the transcription or expression of said nucleotide sequence. Those skilled in the art will appreciate that the control sequences (e.g., promoter) need not be contiguous with the nucleotide sequence to which it is operably associated, as long as the control sequences function to direct the expression thereof. Thus, for example, intervening untranslated, yet transcribed, sequences can be present between a promoter and a nucleotide sequence, and the promoter can still be considered “operably linked” to the nucleotide sequence. 
     A “promoter” is a nucleotide sequence that controls or regulates the transcription of a nucleotide sequence (i.e., a coding sequence) that is operably associated with the promoter. The coding sequence may encode a polypeptide and/or a functional RNA. Typically, a “promoter” refers to a nucleotide sequence that contains a binding site for RNA polymerase II and directs the initiation of transcription. In general, promoters are found 5′, or upstream, relative to the start of the coding region of the corresponding coding sequence. The promoter region may comprise other elements that act as regulators of gene expression. These include a TATA box consensus sequence, and often a CAAT box consensus sequence (Breathnach and Chambon, (1981)  Annu. Rev. Biochem.  50:349). 
     Promoters can include, for example, constitutive, inducible, temporally regulated, developmentally regulated, chemically regulated, tissue-preferred and/or tissue-specific promoters for use in the preparation of recombinant nucleic acid molecules, i.e., “chimeric genes” or “chimeric polynucleotides.” In particular aspects, a “promoter” useful with the invention is a promoter capable of initiating transcription of a nucleotide sequence in a yeast cell. 
     The choice of promoter will vary depending on the temporal and spatial requirements for expression, and also depending on the host cell to be transformed. Promoters useful with the invention include, but are not limited to, those that drive expression of a nucleotide sequence constitutively, those that drive expression when induced, and those that drive expression in a tissue- or developmentally-specific manner. These various types of promoters are known in the art. 
     As used herein the terms “fatty acid derivative” or “fatty acid derivatives” includes but are not limited to hydrocarbons, such as for example alkanes and/or alkenes as well as fatty alcohols, of any length (e.g., short, medium and long chain). 
     Hexadecenal dehydrogenase gene HFD1 is found in  Saccharomyces cerevisiae  and encodes hexadecenal dehydrogenase Hfd1. HFD1 homologues can be found in other yeasts and are also envisioned to be part of this invention even though the gene name may not be the same. 
     Acyl-CoA or fatty acyl-CoA is a group of molecules involved in the metabolism of fatty acids. It is a transient intermediate compound formed when coenzyme A (CoA) attaches to the end of a fatty acid inside living cells. 
     ACP (acyl carrier protein) is a protein that covalently binds fatty acyl intermediates via a phosphopantetheine linker during the synthesis process. 
     Fatty acid derivatives (e.g., alkanes, alkenes and/or fatty alcohols, and the like) may be produced in yeasts by conversion of acyl coenzyme A (acyl-CoA), fatty acids, or fatty acyl-ACP. Several pathways may be used to get the yeasts to produce acyl-CoA, fatty acids, fatty acyl-ACP. However the production of the fatty acid derivatives from acyl-CoA, fatty acids and/or fatty acyl-ACP via fatty aldehydes will only be possible to a substantial extend if HDF1 is deleted. 
     An aspect of the embodiments relates to a yeast lacking a gene encoding hexadecanal dehydrogenase (HFD1) or comprising a disrupted gene encoding HFD1. The yeast also comprises at least one heterologous gene encoding an enzyme involved in a pathway of producing hydrocarbons. 
     In an embodiment, the yeast comprises at least one heterologous gene encoding an enzyme involved in a pathway of producing hydrocarbons from fatty acyl-CoA through fatty aldehydes. 
     In an embodiment, the yeast comprises a heterologous gene encoding a fatty acyl-CoA reductase or a fatty acyl-Acyl Carrier Protein (ACP) reductase, preferably  Synechococcus elongates  orf1594 or  Acinetobacter baylyi  Acr1. 
     In an embodiment, the yeast comprises a heterologous gene encoding a fatty aldehyde-deformylating oxygenase, preferably  Synechococcus elongates  orf1593 or  Nostoc puntiforme  fatty aldehyde-deformylating oxygenase. 
     In a particular embodiment, the heterologous gene is a fusion gene encoding a fusion of said fatty aldehyde-deformylating oxygenase and a catalase. 
     In a particular embodiment the yeast further comprises a heterologous gene encoding cytosolic ferredoxin, preferably  Escherichia coli  fdx or  Synechococcus elongates  petF, and a heterologous gene encoding a cytosolic ferredoxin nicotinamide adenine dinucleotide phosphate (NADP+) reductase and/or a cytosolic ferredoxin NAD+ reductase, preferably  E. coli  fdr or  S. elongates  petH and/or an  E. coli  or  S. elongates  ferredoxin NAD+ reductase. 
     In an embodiment, the yeast comprises a heterologous gene encoding  Acinetobacter baylyi  Acr1, a heterologous gene encoding  Musca domestica  CYP4G2 deformylating oxygenase, and a heterologous gene encoding  M. domestica  NADPH-cytochrome P450 reductase. 
     In an embodiment, the yeast comprises a heterologous gene encoding  Jeotgalicoccus  spp Orf880. 
     In a particular embodiment, the yeast further comprises a heterologous gene encoding a chaperon selected from a group consisting of  Escherichia coli  GroEL and  E. coli  GroES. 
     In an embodiment, the yeast comprises  Photorhabdus luminescens  genes LuxC, LuxD and LuxE, and a cyanobacterial fatty aldehyde-deformylating oxygenase, preferably  Synechococcus elongates  orf1593 or  Nostoc puntiforme  fatty aldehyde-deformylating oxygenase. 
     In an embodiment, the yeast comprises a heterologous gene encoding  Mycobacterium marinum  carboxylic acid reductase, a heterologous gene encoding  Musca domestica  CYP4G2 deformylating oxygenase, and a heterologous gene encoding a phosphopantetheinyl transferase, preferably  Aspergillus nidulans  phosphopantetheinyl transferase. 
     In an embodiment, the yeast comprises a heterologous gene encoding a fatty acyl-Acyl Carrier Protein (ACP) synthase, preferably  Synechococcus elongates  fatty acyl-ACP synthase, a heterologous gene encoding a fatty acyl-ACP reductase, preferably  Synechococcus elongates  orf1594, a heterologous gene encoding  Musca domestica  CYP4G2 decarbonylase, and a heterologous gene encoding  M. domestica  NADPH-cytochrome P450 reductase. 
     In an embodiment, the yeast comprises a heterologous gene encoding a fatty acid reductase and a mitochondrial localization signal (MLS), preferably  Mycobacterium marinum  CAR fatty acid reductase and the MLS, a heterologous gene encoding a fatty aldehyde decarbonylase and the MLS, preferably  Nostoc punctiforme  fatty aldehyde-deformylating oxygenase and the MLS, and a heterologous gene encoding a phosphopantetheinyl transferase and the MLS, preferably  Aspergillus nidulans  phosphopantetheinyl transferase and the MLS. 
     In a particular embodiment, the yeast further comprises at least one gene encoding a respective enzyme involved in the yeast mitochondrial fatty acid biosynthetic pathway selected from the group consisting of a yeast mitochondrial 2-enoyl thioester reductase and a yeast mitochondrial acetyl-Coenzyme A (CoA) carboxylase, a yeast mitochondrial beta-keto-acyl synthase, a yeast mitochondrial 3-hydroxyacyl-Acyl Carrier Protein (ACP) dehydratase, a yeast mitochondrial 3-oxoacyl-ACP reductase, and a yeast mitochondrial malonyl-CoA:ACP transferase, preferably selected from the group consisting of  Saccharomyces cerevisiae  HFA1, ETR1, CEM1, HTD2, OAR1 and MCT1. 
     In a particular embodiment, the yeast further comprises a heterologous gene encoding a mitochondrial thoesterase, preferably selected from the group consisting of  Acinetobacter baylyi  TesA and  Cocos nucifera  FatB 1. 
     In an embodiment, the yeast comprises a gene encoding a mitochondrial formate dehydrogenase, preferably an endogenous format dehydrogenase and a mitochondrial localization signal (MLS), more preferably  Saccharomyces cerevisiae  FDH1 and/or FDH2 and the MLS. 
     In an embodiment, the yeast comprises at least one heterologous gene encoding cytosolic enzyme selected from the group consisting of acetyl-Coenzyme A (CoA) C-acetyltransferase, a 3-ketoacyl-CoA thiolase, a 3-hydroxyacyl-CoA dehydrogenase, an enoyl-CoA hydratase, a trans-enoyl-CoA reductase and a thioesterase, preferably selected from the group consisting of  Saccharomyces cerevisiae  FOX2, FOX3, ERG10 and TES1 and bacterial yqeF, fadA, fabB and tdTER. 
     In an embodiment, the yeast comprises a heterologous gene encoding a thioesterase, preferably selected from the group consisting of  Escherichia coli  tesA, tesB, fadM and yciA. 
     In an embodiment the yeast lacks or has reduced non-essential storage lipid formation, preferably by lacking one or more genes selected from the group consisting of any acyl-Coenzyme A (CoA):sterol acyltransferase and any diacylgylcerol acyltransferase, more preferably by lacking one or more of  Saccharomyces cerevisiae  LRO1, DGA1, ARE1 and ARE2, or comprising one or more disrupted genes selected from the group. 
     In an embodiment, the yeast lacks or has reduced non-essential beta oxidation, preferably by lacking one or more genes selected from the group consisting of any peroxisomal fatty acyl-Coenzyme A (CoA) oxidase and any long chain fatty acyl-CoA synthetase, more preferably by lacking one or more of  Saccharomyces cerevisiae  FAA1, FAA4 and PDX1, or comprising one or more disrupted genes selected from the group. 
     In an embodiment, the yeast comprises genes adapted for overexpression enzymes involved in the fatty acid biosynthetic pathway selected from the group consisting of acetyl-Coenzyme A (CoA) carboxylase and fatty acid synthase, preferably  Saccharomyces cerevisiae  ACC1, FAS1, FAS2 and ACB1. 
     In an embodiment, the yeast comprises heterologous genes adapted for overexpression enzymes involved in the fatty acid biosynthetic pathway selected from the group consisting of acetyl-Coenzyme A (CoA) carboxylase and fatty acid synthase, preferably  Rhodosporidium toruloides  RtACC1, RtFAS1 and RtFAS2. 
     In an embodiment, the yeast is characterized by supply of nicotinamide adenine dinucleotide phosphate (NADPH) by: 
     comprising a heterologous gene encoding a non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, preferably  Streptococcus mutans  GAPN; 
     lacking an endogenous GDH1 gene encoding NAD-dependent glutamate dehydrogenase, or comprising a disrupted GDH1 gene; and/or 
     comprising a GDH2 gene adapted for overexpression of NAD-dependent glutamate dehydrogenase. 
     In an embodiment, the yeast is selected from the group consisting of a  Saccharomyces  yeast,  Hansenula polymorpha , a  Kluyveromyces  yeast, a  Pichia  yeast, a  Candida  yeast, a  Trichoderma  yeast and  Yarrowia lipolytica , preferably  Saccharomyces cerevisiae.    
     Another aspect of the embodiments relates to a method for producing hydrocarbons. The method comprises culturing a yeast lacking a gene encoding hexadecenal dehydrogenase (HFD1) or comprising a disrupted gene encoding HFD1 in culture conditions suitable for production of the hydrocarbons from the yeast. The method also comprises collecting the hydrocarbons from the culture medium in which the yeast is cultured and/or from the yeast. 
     In an embodiment, culturing the yeast comprises culturing a yeast according to any of the embodiments in the culture conditions suitable for production of the hydrocarbons from the yeast. 
     In an embodiment, the hydrocarbons are a fatty acid derivative selected from a group consisting of an alkane, an alkene and a fatty alcohol, preferably selected from the group consisting of an alkane and an alkene. 
     A further aspect of the embodiments relates to use of a yeast lacking a gene encoding hexadecenal dehydrogenase (HFD1) or comprising a disrupted gene encoding HFD1 for the production of hydrocarbons. 
     In an embodiment, the yeast is according to any of the embodiments. 
     In an embodiment, the hydrocarbons are a fatty acid derivative selected from a group consisting of an alkane, an alkene and a fatty alcohol, preferably selected from the group consisting of an alkane and an alkene. 
     The invention will now be described with reference to the following examples. It should be appreciated that these examples are not intended to limit the scope of the claims to the invention, but are rather intended to be exemplary of certain embodiments. Any variations in the exemplified methods that occur to the skilled artisan are intended to fall within the scope of the invention. 
     EXAMPLES 
     Example 1 
     Expression of an Alkane Biosynthetic Pathway in  Saccharomyces cerevisiae  hfd1Δ 
     The purpose of this example is to illustrate the importance of HFD1 deletion in yeast to enable, for example, alkane, alkene and fatty alcohol biosynthesis via a two-step pathway involving a fatty aldehyde as intermediate. As a proof of principal, a commercially available knock-out strain  Saccharomyces cerevisiae  BY4741 6550 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hfd1Δ) was transformed with the plasmids pAlkane0 and pKB02 carrying the  Synechococcus elongatus  fatty acyl-CoA/ACP reductase gene orf1594 and fatty aldehyde decarbonylase gene orf1593, and  Escherichia coli  DH5 ferredoxin gene fdx and ferredoxin reductase gene fpr. A control strain harboring two empty plasmids and a wild-type BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) strain (harboring the same plasmids as the producer strain) were constructed simultaneously. 
     The genes orf1594 (NT ID 1, codon-optimized for yeast) and orf1593 (NT ID 2, idem) coding for the two-step cyanobacterial alkane biosynthetic pathway described by Schirmer et al (2010) were ordered codon-optimized for yeast from GenScript (Piscataway, N.J., USA). Orf1594 was flanked by the restriction sites BamHI/HindIII, and orf1593 by NotI/SacI. The genes were cloned into pSPGM1 (Chen et al, 2012) by restriction, ligation, and amplification in  Escherichia coli  DH5α resulting in plasmid pAlkane0. The  Escherichia coli  DH5α fdx (NT ID 8) was cloned from a single colony by PCR using the primers PR ID 158 and PR ID 159. These primers contained the restriction site NotI/SacI. The gene fpr (NT ID 9), flanked by the restriction sites BamHII/XhoI, was cut from the plasmid pISPO8 (Partow et al, 2012). Both genes were cloned into pIYCO4 (Chen et al, 2013) by restriction, ligation, and amplification in  Escherichia coli  DH5α resulting in plasmid KB02. Both plasmids were verified by restriction analysis and sequencing of each gene (PR ID 187-190). After verification, both plasmids were co-transformed into chemical competent yeast cells (Gietz et al, 2002). 
     Four independent clones were isolated for both the producer and control strain by streak purification onto fresh SD-His-Ura 2% glucose plates. Successful transformation of the producer was verified by colony PCR (using primers PR ID 150-151, 154-155, and 158-161). Each clone was grown overnight in a 5 ml YPD (yeast peptone dextrose) pre-culture and inoculated the next day at 0.2 OD in 25 ml 2% glucose synthetic medium (dropout uracil and histidine) in 250 ml shake flasks. The cultures were incubated at 30° C. and 200 rpm. After 48 h, cell pellets were collected by centrifugation 5 minutes at 1000 rcf, washed twice with 5 ml phosphate buffer (10 mM KH 2 PO 4 , pH 7.5). Extraction of lipids and alkanes was carried out as described by Khoomrung et al (2013), with the exception that the final sample was dissolved in hexane (instead of chloroform/methanol). Subsequently, 2 μl injections were analyzed using a gas chromatograph (Focus GC, ThermoScientific) mass spectrometer (DSQII ThermoScientific) equipped with a ZB-5MS Guardian (L=30 m, ID 0.25 mm, df=0.25 μm, Phenomenex) column. The inlet temperature was set to 250° C., the helium (carrier) gas flow to 1 ml/min splitless. The initial oven temperature was set to 50° C. and held for 5 minutes, then the temperature was ramped to 310° C. by 10° C./min and held for 6 minutes. The mass transfer line temperature was set to 300° C., the ion source temperature was set to 230° C. and a full scan for m/z of 50 to 650 was performed. 
     A gas chromatogram spectrum of one independent clone of the producing strain, one control, and a standard run is shown in  FIG. 5 . In  FIG. 4  another spectrum is shown for another independent clone of the producing strain, one wild-type strain harboring the pathway, and a standard run. These figures illustrate that HFD1 is required to enable alkane production in  Saccharomyces cerevisiae.    
     Example 2 
     Deletion of Hexadecenal Dehydrogenase HFD1 in  Saccharomyces cerevisiae  CEN.PK113-11C 
     The purpose of this example is to show how HFD1 was deleted in  Saccharomyces cerevisiae  CEN.PK113-11C which is a commercially available strain. The yeast  Saccharomyces cerevisiae  possesses hexadecenal dehydrogenase Hfd1, an enzyme which will compete for substrate with the heterologous fatty aldehyde decarbonylases and leads to an ATP consuming futile cycle. In cyanobacteria, it has been shown that deletion of a similar gene led to fatty aldehyde accumulation.  Saccharomyces cerevisiae  HFD1 was deleted using the strategy depicted in  FIG. 6 . Using two primer pairs (PR ID 122-125) up and downstream fragments of HFD1 were cloned, and using primer pair PR ID 127-128  Kluyveromyces lactis  URA3 was cloned from plasmid pWJ1042 (Reid et al., 2002). Subsequently all three fragments were fused using primer pair (PR ID 122 and 125) as described Zhou et al., 2012. The deletion cassette was transformed into  Saccharomyces cerevisiae  CEN.PK113-11C (MATa MAL2-8c SUC2 his3Δ1 ura 3-52) by electroporation at 1.5 kV, 10 μF, and 200Ω in a 0.2 cm gap electroporation cuvette using Bio-Rad MicroPulser electroporation apparatus (Bio-Rad Laboratories AB, Sweden) and selected on URA drop out plates for integration. Transformants were verified by colony PCR and the KlURA3 marker was subsequently looped out using flanking direct repeats as illustrated in  FIG. 6 . Successful clones were selected by growth on 5-FOA and URA dropout plates. 
     Example 3 
     Expression of  Escherichia coli  Ferredoxin and  Escherichia coli  Ferredoxin:NADPH Reductase in  Saccharomyces cerevisiae    
     It has been shown that cyanobacterial fatty aldehyde decarbonylases require an electron transfer system and that  Escherichia coli  ferredoxin and ferredoxin:NADPH reductase can be used as such. The yeast  Saccharomyces cerevisiae  contains ferredoxin and ferredoxin:NADPH reductase homologues (Yah1 and Arh1, respectively), but they are localized to the mitochondria and can therefore most likely not be used by the cytosolic expressed fatty aldehyde decarbonylase. The  Escherichia coli  DH5α fdx (NT ID 8) was cloned from a single colony by PCR using the primers PR ID 212 and PR ID 213. The gene fpr (NT ID 9), was cloned from the plasmid pISP08 (Partow et al, 2012) by PCR using the primers PR ID 214 and PR ID 215.To enable alka/ene biosynthesis, this plasmid carries a fatty acid reductase and fatty aldehyde decarbonylase homologous (as described in Example 1; cloned using primers PR ID 208-211). Combinations of these genes were introduced into pYX212 by using a modular pathway engineering strategy as described before (Zhou et al., 2012), resulting in the plasmids pAlkane1, pAlkane 7, pAlkane 8, and pFAR see  FIG. 13 . Plasmids were extracted from single yeast colonies using the Zymoprep Yeast Plasmid Miniprep II kit (Nordic Biolabs, Taby, Sweden) and transformed into  E. coli  DH5α competent cells. After purification of the plasmid, verification by restriction analysis, and sequencing, the plasmids were transformed into  Saccharomyces cerevisiae  CEN.PK113-11C and  Saccharomyces cerevisiae  hfd1Δ. Yeast competent cells were prepared and transformed with 1 μg of plasmid according to the lithium acetate/single-stranded carrier DNA/polyethylene glycol method (Gietz and Woods, 2002) and successful transformants were selected on URA dropout plates. 
     Shake flask batch fermentations were carried out in minimal medium containing 30 g/l glucose (Verduyn et al., 1992). Cultures were inoculated, from overnight precultures, at 0.1 OD in 25 ml minimal medium supplemented with histidine (40 mg/l; Sigma Aldrich) in 250 ml shake flasks. The shake flasks were incubated at 30° C. and 200 rpm orbital shaking. After 48 hours the cells were harvested by centrifugation (5 minutes; 1000 g) and washed once with 5 ml phosphate buffer (10 mM KH2PO4, pH 7.5). The supernatant was removed, the pellet frozen in liquid nitrogen and freeze dried (Christ Alpha 2-4 LSC, Martin Christ Gefriertrocknungsanlagen GmbH, Osterode am Harz, Germany) for 48 hours. Alkanes were extracted from the freeze dried cell pellets as described before (Khoomrung et al., 2013), with the exceptions that the extracted fraction was dissolved in hexane (alkanes) and that hexadecane (alkanes) was used as an internal standard. Samples were analyzed by gas chromatography (FocusGC, ThermoFisher Scientific) coupled to mass spectrometry (DSQII, ThermoFisher Scientific) using a Zebron ZB-5MS Guardian capillary GC column (30 m×0.25 mm×0.25 Phenomenex, Værløse, Denmark). The GC-MS conditions are described in Example 1. Analytical standards for alkanes (Sigma Aldrich) were analyzed during the same run for peak identification and quantification. The alkane production levels as observed for the wild-type and hfd1Δ strains carrying the plasmid pAlkane1 (KB 17 and KB 19), pAlkane 7 (KB 18), or pAlkane 8 (KB 16) is shown in  FIG. 2A . This figure illustrates that expression of a ferredoxin/ferredoxin reductase reducing system is required to enable alkane production in  Saccharomyces cerevisiae  CEN.PK. The gas chromatogram spectra of  Saccharomyces cerevisiae  CEN.PK113-11C and  Saccharomyces cerevisiae  hfd1Δ expressing the plasmid pAlkane1, pAlkane7, or pAlkane8 are further shown in  FIG. 3 . 
     Example 4 
     Expression of  Synechococcus elongatus  PCC7942 Ferredoxin and  Synechococcus elongatus  Ferredoxin: NADPH Reductase in  Saccharomyces cerevisiae    
     Recently the endogenous  Synechococcus elongatus  electron transfer system was identified and shown to be more efficient in vitro than the heterologous system. The  Synechococcus elongatus  PCC7942 ferredoxin (orf_1499, petF, P ID 6) and ferredoxin-NADPH reductase (orf_0978, petH, P ID 7) genes are codon optimized for expression in yeast. Subsequently they are cloned similar to the  E coli  homologues, as described in example 3, and cotransformed with fatty aldehyde decarbonylase homologue carrying plasmid as described in example 1. 
     Example 5 
     Conversion of Fatty Acyl-CoA to Alka/enes by Expression of  Acinetobacter baylyi  Acr1 and  Musca domestica  CYP4G2 Decarbonylase in  Saccharomyces cerevisiae  hfd1Δ 
     The purpose of this example is to illustrate the possibility of expression of a fatty acyl-CoA preferring fatty acid reductase in combination with a P450 type decarbonylase. Thus this pathway will convert fatty acyl-CoA to alkanes and alkenes via the intermediates fatty acyl-CoA and fatty aldehydes. 
     The plasmid pAlkane3 was constructed similar to the method described in example 8. For expression in yeast codon optimized genes encoding  Acinetobacter baylyi  Acr1 (NT ID 22),  Musca domestica  CYP4G2 (NT ID 14), and  Musca domestica  NADPH-cytochrome P450 reductase (NT ID 15) were cloned using primers with to the gene homologous regions (PR ID 201-202, 196-197, 192-193). The pathway was subsequently assembled as described in Shao et al, 2009 and Zhou et al, 2012. 
     Cells were cultivated and analyzed as described in example 1. 
     Example 6 
     Conversion of Fatty Acids to Terminal Alkenes by Expression of  Jeotgalicoccus  spp orf880,  Escherichia coli  GroEL and  Escherichia coli  GroES in  Saccharomyces cerevisiae    
     The purpose of this example is to illustrate the improvement of conversion efficiency of the decarboxylation pathway by expression of chaperones. This will improve the folding of  Jeotgalicoccus  spp Orf880p. Overexpression of GroEL and GroES is done e.g. according to Guadelupe-Medina et al, 2013 on a HIS marker plasmid (e.g. pIYC04). 
     The gene  Jeotgalicoccus  spp Orf880 (NT ID 4, codon-optimized for yeast) coding for the one-step cyanobacterial alkane biosynthetic pathway was ordered codon-optimized for yeast from GenScript (Piscataway, N.J., USA). The gene was flanked by the restriction sites NotI/SacI and it was cloned into pSP-GM1 (Chen et al, 2012) by restriction, ligation, and amplification in  Escherichia coli  DH5α. The resulting plasmid OleT was verified by restriction analysis and sequencing (PR ID 188-189). After verification, the plasmids were cotransformed into chemical competent yeast cells (Gietz et al, 2002). 
     Cells are cultivated and analyzed as described in example 1. 
     Example 7 
     Conversion of Acyl-CoA to Alka/enes by Expression of  Escherichia coli  TesA′,  Photorhabdus luminescens  LuxC, LuxD, and LuxE, and  Nostoc punctiforme  FAD in  Saccharomyces cerevisiae  hfd1Δ 
     This invention demonstrates the utilization of fatty acyl-CoA for the synthesis of alkanes and alkenes (see  FIG. 14 ) using (part of) the bacterial luminescence pathway and a cyanobacterial fatty aldehyde decarbonylase. The expression of a thioesterase might relieve the inhibitory effect of fatty acyl-CoA on fatty acid synthesis and will provide the substrate of the enzymes LuxC, LuxD, and LuxE. 
     The  Photorhabdus luminescens  genes encoding LuxC (P ID 3), LuxD (P ID 4), and LuxE (P ID 5) were codon-optimized for expression in yeast, and cloned using primers PR ID 212-217. A pathway consisting of these three genes, a  Synechoccous elongatus  (NT ID 2, cloned using primers PR ID 220-221) or a  Nostoc punctiforme  FAD gene (NT ID 3, cloned using primers PR ID 218-219), and  Escherichia coli  truncated thioesterase TesA (NT ID 56, cloned using primers PR ID) is assembled on a plasmid pAlkane8 and pAlkane5 similar to the method described in examples 3. The transformation of the plasmids into CEN.PK113-11C hfd1Δ was carried out according to Gietz et al, 2002. Cells were cultivated and analyzed as described in example 1. 
     The gas chromatogram spectra as observed for the hfd1Δ strain carrying the plasmid pAlkane5 (carrying the  Nostoc punctiforme  FAD) or pAlkane9 (carrying the  Synechoccous elongatus  FAD) are shown in  FIG. 15 . This figure illustrates that expression of a bacterial luminescence pathway and a cyanobacterial fatty aldehyde decarbonylase enables alkane production in  Saccharomyces cerevisiae  CEN.PK. 
     Example 8 
     Conversion of Fatty Acids to Alka/enes by Expression of  Mycobacterium marinum  Carboxylic Acid Reductase and  Musca domestica  CYP4G2 Decarbonylase in  Saccharomyces cerevisiae  hfd1Δ 
     In this invention the  Mycobacterium marinum  carboxylic acid reductase (NT ID 7) was expressed in  Saccharomyces cerevisiae  CEN.PK113-11C hfd1Δ to convert fatty acids to fatty aldehydes. The  Musca domestica  CYP4G2 P450 decarbonylase (NT ID 14) enzyme was also expressed to subsequently convert these fatty aldehydes into alka/enes. The plasmid pAlkane4 was constructed by cloning the for yeast codon optimized genes encoding  Mycobacterium marinum  CAR (NT ID 14),  Musca domestica  CYP4G2 (NT ID 14),  Musca domestica  NADPH-cytochrome P450 reductase (NT ID 15), and the  Aspergillus nidulans  phosphopantetheinyl transferase NpgA (NT ID 5) with overlap primers (PR ID114-115, 112-113, 192-193 and 108-109, respectively). The pathway was subsequently assembled as described in Shao et al, 2009 and Zhou et al, 2012.Cells were cultivated and analyzed as described in example 1. In addition to these four enzymes, an additional thioesterase is expressed to relieve fatty acid biosynthesis repression by acyl-CoA and to increase substrate availability for this pathway. 
     Example 9 
     Conversion of Fatty Acids to Alka/enes by Expression of  Synechococcus elongatus  PCC7942 ACS,  Synechococcus elongatus  PCC7942 orf1594 and  Musca domestica  CYP4G2 Decarbonylase in  Saccharomyces cerevisiae  hfd1Δ 
     The purpose of this example is to illustrate the possibility of expression of a fatty acyl-ACP synthase to provide more of the preferred substrate acyl-ACP for the fatty acyl-ACP reductase, and the combination of a P450 type decarbonylase and cyanobacterial reductase. Thus this pathway will convert fatty acids to alkanes and alkenes via the intermediates fatty acyl-ACP and fatty aldehydes. 
     The plasmid pAlkane2 was constructed similar to the method described in example 8. For expression in yeast codon optimized genes encoding  Synechococcus elongatus  PCC7942 orf1594 (NT ID 1),  Musca domestica  CYP4G2 (NT ID 14),  Musca domestica  NADPH-cytochrome P450 reductase (NT ID 15), and  Synechococcus elongatus  ACS (NT ID 6?) were cloned using primers with to the gene homologous regions (PR ID 194-195, 196-197, 192-193, 110-111, respectively). The pathway was subsequently assembled as described in Shao et al, 2009 and Zhou et al, 2012. 
     Cells were cultivated and analyzed as described in example 1. In addition to these four genes, an additional thioesterase with preference for acyl-CoA over acyl-ACP is expressed to increase the levels of free fatty acids. 
     Example 10 
     Fusing of  Nostoc punctiforme  Fatty Aldehyde Decarbonylase to Catalase and Expression in  Saccharomyces cerevisiae  hfd1Δ for Improved Fatty Aldehyde to Alka/ene Conversion 
     The purpose of this invention is to improve the catalytic activity of the fatty aldehyde decarbonylase, which can be the  Synechoccocus elongatus  PCC7942 orf1593 (NT ID 2) or the  Nostoc punctiforme  FAD (NT ID 3), or a homologue. 
     The fatty aldehyde decarbonylase can be fused to a catalase as has been shown by Andre et al (2013). This will improve the activity of this enzyme and thus the alka/ene formation. The proposed mechanism is that the toxic byproduct hydrogen peroxide is broken down by the catalase, thereby avoiding that it can inhibit the decarbonylase. The novelty would be to express such a fusion enzyme in yeast together with HFD1 deletion. A heterologously expressed fatty acid reductase, as described in, for example, example 8, and the endogenous fatty acid synthesis via the breakdown of spingholipids, can supply the fatty aldehydes for the decarbonylase-catalase fusion enzyme. 
     Example 11 
     Expression of Alkane or Alkene Biosynthetic Pathway in the Mitochondria of  Saccharomyces cerevisiae    
     The purpose of this example is to illustrate the utilization of the mitochondrial fatty acid biosynthetic machinery for the synthesis of short chain fatty acids, and its subsequent conversion into short chain alkanes and alkenes. 
     In this experiment the  Mycobacterium marinum  CAR (NT ID 7) fatty acid reductase and the  Nostoc puntiforme  (NT ID 3) fatty aldehyde decarbonylase encoding genes were expressed in the mitochondria of  Saccharomyces cerevisiae  CEN.PK113-11C. All enzymes not localized by default into the mitochondria were directed there by attaching a mitochondrial localization signal (Hurt et al, 1985) to the front of each gene. In addition to the alkane biosynthetic pathway, the genes encoding key components of the mitochondrial fatty acid machinery Etr1 (2-enoyl thioester reductase) and Hfa1 (acetyl-CoA carboxylase) were overexpressed to ensure sufficient precursor supply for the alkane pathway. 
     The plasmid pAlkane6 was constructed similar to the method described in example 5, 8 and 9. For expression in yeast codon optimized genes encoding  Mycobacterium marinum  CAR (NT ID 14, attached MLS),  Nostoc punctiforme  FAD (NT ID 3, attached MLS),  Aspergillus nidulans  phosphopantetheinyl transferase NpgA (NT ID 5, attached MLS),  Saccharomyces cerevisiae  Hfa1 (NT ID 61), and  Saccharomyces cerevisiae  Etr1 (NT ID 60) were cloned using primers with to the gene homologous regions (PR ID 165-178, respectively, HFA1 was split up in three parts due to its length). The pathway was subsequently assembled as described in Shao et al, 2009 and Zhou et al, 2012. 
       Escherichia coli  fdx (NT ID 8) and fpr (NT ID 9) were cloned from  Escherichia coli  DH5α genomic DNA, a mitochondrial localization signal (Hurt et al, 1985) was included in the forward primers in front of each gene, and the resulting gene fragments were ligated into the plasmid pIYCO4 (Chen et al, 2012). The resulting plasmid, KB03, was verified by sequencing using primers PR ID 187-190. Subsequently the pAlkane6 and pKB03 plasmids were transformed into  Saccharomyces cerevisiae  CEN.PK113-11C by chemical transformation (Gietz et al, 2002) and successful transformants were selected on HIS dropout plates. To enable alkalene biosynthesis, this plasmid can be co-transformed with a plasmid carrying fatty acid reductase and fatty aldehyde decarbonylase homologous and auxiliary enzymes. 
     Precursor supply can possibly be enhanced by removing post translational modification sites in Etr1 (K301) and Hfa1 (1157S), and by further overexpression of the remaining fatty acid biosynthetic enzymes (e.g. Cem1, Htd2, Oar1, and Mct1). 
     Expression of a thioesterase is required to provide sufficient precursors to the mitochondrial alkane pathway since there is no known yeast mitochondrial thioesterase with activity towards medium chain fatty acyl-ACP.  Acinetobacter baylyi  TesA (P ID 2),  Cocos nucifera  FatB 1 (P ID 1), or homologue thioesterases have been shown to have preference for C8-C14 fatty acyl-ACPs. A thioesterase gene will be codon-optimized for expression in yeast, and subsequently expressed and directed to the mitochondria in a similar fashion as described above. 
     Example 12 
     Expression of Mitochondrial Formate Dehydrogenase in  Saccharomyces cerevisiae    
     Yeast contains a formate dehydrogenase enzyme which is localized to the cytosol. Expression of formate dehydrogenase in the mitochondria might be required to breakdown the toxic byproduct formate of the decarbonylation reaction. Overexpression of endogenous formate dehydrogenase Fdh1 and/or Fdh2 and localization of these proteins to the mitochondria can be achieved by introducing a 5′ mitochondrial localization signal into each gene (as has been described for others genes in example 11). 
     Example 13 
     Construction of a Cytosolic Pathway for Medium-Chain Saturated Fatty Acid Production in  Saccharomyces cerevisiae    
     This chimeric cytosolic pathway, composed of an acetyl-CoA C-acetyltransferase (YqeF or Erg10p), a 3-ketoacyl-CoA thiolase (FadA or Fox3p), a 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase multifunctional enzyme (FadB or Fox2p), a trans-enoyl-CoA reductase (tdTER) and a thioesterase (Tes1p) ( FIG. 7 ), allows the increased total production of medium-chain fatty acids from cytosolic acetyl-CoA as well as an increase in the medium-chain/long-chain fatty acid production ratio. This was shown by analysis of produced fatty alcohols after transforming the constructed strains with a “fatty-acid to fatty alcohol” pathway ( FIG. 8 ). It can also be coupled with different thioesterase homologues (with different chain-length specificities) as terminator enzymes (see example 14) for regulation of the desired fatty acid chain-length. Yeast genes FOX2 (NT ID 48), FOX3 (NT ID 49), ERG10 (NT ID 50) and TES1 (NT ID 51) were amplified by PCR from genomic DNA extracted from  S. cerevisiae  strain CEN.PK 113-11C, using the primers PR ID 80 to 87. These primers were designed to amplify these genes excluding the correspondent peroxisome-targeting signal peptide present in FOX2, FOX3 and TES1. Truncated genes lacking the sequence coding for the signal peptide were then named FOX2c, FOX3c and TES1c respectively. The bacterial genes yqeF (NT ID 54), fadA (NT ID 52), fadB (NT ID 53) and tdTER (NT ID 55) were optimized for expression in  S. cerevisiae  and synthesized by GenScript (Piscataway, N.J., USA). These bacterial genes were amplified using primers PR ID 72-79. The primers from PR ID 69-87 allow the cloning of the genes with the pPGK1 promoter or the bidirectional promoter pPGK1-pTEF1 in the pX-2-loxP-KlURA3, pXI-3-loxP-URA3 and pXI-5-loxp-Sphis5 vectors (Mikkelsen et al, 2012) following the USER cloning method (Nour-Eldin et al, 2006). Primers PR ID 69 and 70 were used to amplify the bidirectional promoter pPGK1-pTEF1 from pSP-GM1, primers PR ID 71 and 70 were used to amplify the pPGK1 promoter also from pSP-GM1. pPGK1-TES1c was cloned into the pX-2-loxP-KlURA3 vector; either fadA-pPGK1-pTEF1-fadB or FOX3c-pPGK1-pTEF1-FOX2c were cloned into pXI-3-loxP-URA3 vector; and either tdTER-pPGK1-pTEF1-yqeF or tdTER-pPGK1-pTEF1-ERG10 were cloned into pXI-5-loxp-Sphis5 vector  FIG. 9 . All the integration constructs were linearized by restriction using NotI restriction enzyme and transformed into a pox1 faa1 faa4 strain (EXAMPLE 16). After integration of the pXI-3-loxP-URA3- and the pXI-5-loxp-Sphis5-derived constructs, the cells were transformed with a Cre recombinase expression plasmid to delete auxotrophy markers by recombination of loxP sites flanking the marker. Next, the originated strain was transformed with the pX-2-loxP-KlURA3 vector containing the pPGK1-TES1 insert. This resulted in the following strains:
     Rbee (pox1Δ faa1Δ faa4Δ yqeF fadA fadB tdTER TES1c)   Rbye (pox1Δ faa1Δ faa4Δ ERG10 fadA fadB tdTER TES1c)   Rbey (pox1Δ faa1Δ faa4Δ yqeF FOX3c FOX2c tdTER TES1c)   Rbyy (pox1Δ faa1Δ faa4Δ ERG10 FOX3c FOX2c tdTER TES1c)   

     Example 14 
     Regulation of Produced Fatty-Acid Chain Length by Expression of Different Thioesterase Genes 
     Different thioesterase homologues have different chain-length specificities. Therefore, coupling of any of the homologues with a fatty-acyl-CoA producing pathway results in production of fatty acids with different chain lengths depending on the thioesterase gene being expressed. Integration of this regulation on an alkane/alkene producing pathway from acetyl-CoA allows production of hydrocarbons with a desired specific chain-length. Thioesterase genes tesA, tesB, fadM or yciA from  E. coli  were used for construction of Rbyy strain (EXAMPLE 13) instead of the TES1c thioesterase gene. The genes tesA (NT ID 56), tesB (NT ID 57), fadM (NT ID 58) and yciA (NT ID 59) were optimized for expression in yeast and synthesized by GenScript (Piscataway, N.J., USA). These genes were amplified using primers PR ID 88 to 95. All the primers used allow the cloning of any of the selected amplified genes with the pPGK1 promoter in the pX-2-loxP-KlURA3 (Mikkelsen et al, 2012) integration vector following the USER cloning method (Nour-Eldin et al, 2006). As explained in EXAMPLE 13, FOX3-pPGK1-pTEF1-FOX2 was cloned into pXI-3-loxP-URA3 vector and tdTER-pPGK1-pTEF1-ERG10 was cloned into pXI-5-loxp-Sphis5 vector. All the integration constructs were linearized by restriction using NotI restriction enzyme and transformed into strain pox1 faa1 faa4 strain (EXAMPLE 16). After integration of the pXI-3-loxP-URA3- and the pXI-5-loxp-Sphis5-derived constructs, the cells were transformed with a Cre recombinase expression plasmid to delete auxotrophy markers by recombination of loxP sites flanking the marker. The originated strain was then transformed with the pX-2-loxP-KlURA3 plasmid containing either pPGK1-tesA, pPGK1-tesB, pPGK1-fadM or pPGK1-yciA. 
     Example 15 
     Expression of Alternative Fatty Acid Synthases for Production of Short/Medium Chain Fatty Acids 
     Expression of a heterologous fatty acid synthase and alternative thioesterase modules as described by Leber and DaSilva (2013) will enable the synthesis of medium chain fatty acids and products derived thereof 
     Example 16 
     Elimination of Storage Lipid Formation (Deletion of LRO1, DGA1, ARE1, ARE2) and Beta-Oxidation (Deletion of PDX1), and Free Fatty Acid Activation (Deletion of FAA1, FAA4) 
     This example describes the elimination of non-essential pathways that consume (activated) fatty acids and thus compete with alkane/alkene production, i.e. storage lipid formation and beta-oxidation. “Activated fatty acid” as used herein means fatty acids coupled to CoA or ACP. 
     For the deletion of ARE1, the 5′ and 3′ ends of the ARE1 open reading frame were individually amplified from genomic DNA of CEN.PK 113-5D (MATa ura3-52) by PCR using primers PR ID 1/2 and PR ID 3/4, respectively. The kanMX expression cassette was amplified in two overlapping parts from plasmid pUG6 (Güldener et al, 1996) using primers PR ID 5/6 and 7/8, respectively. KanMX was looped out as described previously with help of the Cre recombinase expression plasmid pSH47 (Güldener et al, 1996). 
     The same approach was used for deletion of ARE2, DGA1, LRO1, and PDX1. Primers PR ID 9-12 were used for deletion of ARE2, primers PR ID 13-16 were used for deletion of DGA1, primers PR ID 17-20 were used for deletion of LRO1, and primers PR ID 21-24 were used for deletion of POX1. 
     Deletion of FAA1 and FAA4 is e.g. described in Runguphan and Keasling (2013). 
     Example 17 
     Overexpression of Fatty Acid Biosynthetic Genes (ACC1, FAS1, FAS2, ACB1) 
     This example describes the overexpression of genes leading to increased production of (activated) fatty acids. 
     Overexpression of ACC1, FAS1 and FAS2 is e.g. described in Runguphan and Keasling (2013). 
     Mutations S659A and S1157A were introduced into the ACC1 gene by PCR to prevent enzyme regulation by phosphorylation, i.e. to increase enzyme activity. 
     ACB1 (NT ID 47) was amplified by PCR from genomic DNA of  S. cerevisiae  with the oligonucleotide primers PR ID 25/26 and restricted with BamHI/KpnI. The BamHI/KpnI digested DNA fragment was ligated into the BamHI/KpnI sites of vector pSP-GM2 (Partow et al, 2010; Chen et al, 2012) to construct pSP-A. Yeast strains were transformed with the resulting plasmid. 
     Example 18 
     Expression of  Rhodosporidium toruloides  Fatty Acid Biosynthetic Genes ACC1, FAS1, and FAS2 in  Saccharomyces cerevisiae    
     As  Rhodosporidium toruloides  has higher efficiency in lipid production, fatty acid biosynthetic genes RtACC1 (NT ID 19), RtFAS1 (NT ID 20), and RtFAS2 (NT ID 21) from  R. toruloides  can be used for improving the production of fatty acids as well as fatty acid derivatives. The genes were cloned from a cDNA library as described previously (Zhu et al, 2012) with primers pairs RtACC-F (PR ID 120)/RtACC-R (PR ID 121), RtFAS1-F (PR ID 116)/RtFAS1-R (PR ID 117) and RtFAS2-F (PR ID 118)/RtFAS2-R (PR ID 119) and assembled as has been described in Shao et al (2009) and Zhou et al (2012). The expression of RtACC1 and RtFAS1/2, as well their combined expression, increased fatty acid biosynthesis in JV03 ( Saccharomyces cerevisiae  MATa MAL2-8c SUC2 ura3-52 HIS3 are1Δ dga1Δ are2Δ lro1Δ pox1Δ, Valle-Rodriguez et al 2014) ( FIG. 10 ). 
     Example 19 
     Increase of NADPH Supply (GAPN, GDH) 
     This example describes different ways to increase the supply of NADPH, an essential cofactor in fatty acid biosynthesis. 
     Heterologous expression of a non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) from  Streptococcus mutans  is e.g. described in Kocharin et al (2013). 
     Deletion of GDH1 encoding NADP-dependent glutamate dehydrogenase and overexpression of GDH2 encoding NAD-dependent glutamate dehydrogenase is e.g. described in Asadollahi et al (2009). 
     Example 20 
     Conversion of Fatty Acyl-CoA to Fatty Alcohols by Expression of  Marinobacter aquaeolei  VT8 Maqu_2507 Fatty Acyl-CoA Reductase in  Saccharomyces cerevisiae    
     This invention relates to the direct conversion of fatty acyl-CoA into fatty alcohols by a fatty acyl-CoA reductase. 
     The plasmid pAlcohol1 was constructed similar to the method described in example 5, 7, 8, 9. For expression in yeast codon optimized genes  Marinobacter aquaeolei  VT8 Maqu_2507 (NT ID 16) was cloned using primers with to the gene homologous regions (PR ID 206-207). The pathway was subsequently assembled in PYX212 as described in Shao et al, 2009 and Zhou et al, 2012. pAlcohol1 enabled the production of 3.4 mg/L fatty alcohol in  S. cerevisiae  CEN.PK 113-11C in shake flask fermentation. 
     Cells are cultivated and analyzed as described in example 1. 
     Example 21 
     Construction of an Intracellular Alkane Sensor by the Expression of  Yarrowia lipolytica  Yas3 Repressor and Yas1, Yas2 Activator and a Fluorescent Protein Expressed from an ARE1 Containing Promoter in  Saccharomyces cerevisiae.    
     The purpose of this example is to describe the design of an alkane biosensor that can be used to screen for better alkane producer. This can be a strain in which the fatty acid substrate is overproduced (e.g. as described in example 16), or classical mutagenesis experiments to optimize the enzymes of the pathway, or screening of homologue and/or libraries to improve the alkane production. It is based on the negative regulator (Yas3) and two activators (Yas1, Yas2) of alkane metabolism enzymes in the alkane consuming yeast  Yarrowia lipolytica . The repressor Yas3 is released from the alkane response elements (ARE1) in a promoter in the presence of medium chain alkanes. 
     The  Yarrowia  alkane-reponsive promoter of the ALK1 gene was cloned in front of a reporter gene such as GFP to screen for alkane production. Alternatively, the alkane response element was integrated as one or several copies into a  S. cerevisiae  promoter (here the TEF1 promoter) and cloned in front of the reporter gene. For this, a truncated version of the TEF1 promoter was used and combined with three ARE1 binding sites in front of it (NT ID 64). For another strategy three ARE1 binding sites were integrated at specific positions in the complete TEF promoter (NT ID 65). 
     In addition, the  Yarrowia lypolytica  transcriptional activators Yas1 and Yas2 as well as the repressor Yas3 necessary for alkane-mediated transcription regulation will be introduced into  S. cerevisiae  together with the reporter construct. 
     Expressing the repressor gene Yas3 in presence of the two activators Yas1 and Yas2 leads to a 100-fold repression of the green fluorescence reporter signal, indicating the functionality of the system and the sensor range. Exposing the system to alkanes gave a clear response and increased green fluorescence signal, as demonstrated in  FIG. 11 . 
     REFERENCES 
     Andre C, Kim S W, Yu X-H, Shanklin J: Fusing catalase to an alkane-producing enzyme maintains enzymatic activity by converting the inhibitory byproduct H2O2 to the cosubstrate O2. Proc Natl Acad Sci USA 2013, 110:3191-3196 
     Asadollahi M A, Maury J, Patil K R, Schalk M, Clark A, Nielsen J (2009) Enhancing sesquiterpene production in  Saccharomyces cerevisiae  through in silico driven metabolic engineering. Metab Eng 11:328-34 
     Chen, Y., Partow, S., Scalcinati, G., Siewers, V., Nielsen, J., 2012. Enhancing the copy number of episomal plasmids in  Saccharomyces cerevisiae  for improved protein production. FEMS Yeast Res. 12, 598-607 
     Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J (2013) Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng 15:48-54 
     Gietz, R. D., Woods, R. A., 2002. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene-96 
     Güldener U, Heck S, Fiedler T, Beinhauer J, Hegemann J H (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24:2519-2524 
     Hurt, E. C., Pesold-Hurt, B., Suda, K., Oppliger, W., &amp; Schatz, G. (1985). The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix. The EMBO journal, 4(8), 2061-8. Nature Publishing Group 
     Khoomrung S, Chumnanpuen P, Jansa-Ard S, St{dot over (a)}hlman M, Nookaew I, Boren J, Nielsen J. (2013) Rapid quantification of yeast lipid using microwave-assisted total lipid extraction and HPLC-CAD. Anal Chem. 85(10):4912-9 
     Khoomrung S, Chumnanpuen P, Jansa-ard S, Nookaew I, Nielsen J. (2012) Fast and accurate preparation fatty acid methyl esters by microwave-assisted derivatization in the yeast  Saccharomyces cerevisiae . Appl Microbiol Biotechnol. 94(6):1637-46 
     Kocharin K, Siewers V, Nielsen J (2013) Improved polyhydroxybutyrate production by  Saccharomyces cerevisiae  through the use of the phosphoketolase pathway. Biotechnol Bioeng 110:2216-24 
     Leber C, Da Silva N A. (2013) Engineering of  Saccharomyces cerevisiae  for the synthesis of short chain fatty acids. Biotechnol Bioeng.(in press) 
     Mikkelsen M D, Buron L D, Salomonsen B, Olsen C E, Hansen B G, Mortensen U H, Halkier B A. Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform. Metab Eng. 2012 Mar;14(2):104-11 
     Nour-Eldin, H., Hansen, B., Norholm, M., Jensen, J., Halkier, B., (2006). Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments. Nucleic Acids Res. 34, E122 
     Partow S, Siewers V, Bjorn S, Nielsen J, Maury J (2010) Characterization of different promoters for designing a new expression vector in  Saccharomyces cerevisiae . Yeast 27:955-964 
     Partow S, Siewers V, Daviet L, Schalk M, Nielsen J. PLoS One. Reconstruction and evaluation of the synthetic bacterial MEP pathway in  Saccharomyces cerevisiae.  2012;7(12):e52498. doi: 10.1371/journal.pone.0052498. Epub 2012 Dec 28 
     Reid R, Lisby M, Rothstein R. (2002) Cloning-free genome alterations in  Saccharomyces cerevisiae  using adaptamer-mediated PCR. Methods Enzymol 350:258-277 
     Runguphan W, Keasling J D (2013) Metabolic engineering of  Saccharomyces cerevisiae  for production of fatty-acid derived biofuels and chemicals. Metab Eng (in press) 
     Schirmer A, Rude M A, Li X, Popova E, del Cardayre S B: Microbial biosynthesis of alkanes. Science 2010, 329:559-562 
     Shao Z, Zhao H, Zhao H (2009) DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways. Nucleic Acids Res. 37:e16 
     Verduyn C, Postma E, Scheffers W A, Van Dijken J P. 1992. Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8:501-17 
     Zhou Y. J., Gao W., Rong Q., et al. (2012) Modular pathway engineering of diterpenoid synthases and the mevalonic acid pathway for miltiradiene production J. Am. Chem. 134:3234-3241 
     Zhu Z, Zhang S, Liu H, Shen H, Lin X, Yang F, Zhou Y J, Jin G, Ye M, Zou H, Zhao Z K. (2012) A multi-omic map of the lipid-producing yeast  Rhodosporidium toruloides . Nat Commun 3:1112 
     
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Plasmids. 
               
             
          
           
               
                 Plasmid 
                 Backbone 
                 Genes/Characteristics 
                 Source 
               
               
                   
               
               
                 pKB01 
                 PIYC04 
                 EcFLDA, EcFPR 
                   
               
               
                 pKB02 
                 pIYC04 
                 EcFDX, EcFPR 
               
               
                 pKB03 
                 pIYC04 
                 MLS-fdx, MLS-fpr (added 
               
               
                   
                   
                 mitochondrial localization 
               
               
                   
                   
                 signal in front of genes) 
               
               
                 pAlkane0 
                 pSPGM1 
                 SeOrf1594, SeOrf1593 
               
               
                 pOleT 
                 pSPGM1 
                 JOleT/orf880 
               
               
                 pAlkane1 
                 p423GPD 
                 SeOrf1594, SeOrf1593, 
               
               
                   
                   
                 Ecfdx, Ecfpr 
               
               
                 pAlkane2 
                 p423GPD 
                 SeOrf1594, MdP450G2, 
               
               
                   
                   
                 MdCPR, SynAAC 
               
               
                 pAlkane3 
                 p423GPD 
                 AbAcr1, MdP450G2, MdCPR 
               
               
                 pAlkane4 
                   
                 MmCAR, MdP450G2, MdCPR, 
               
               
                   
                   
                 AnnpgA 
               
               
                 pAlkane5 
                 pYX212 
                 PlLuxD, PlLuxC, PlLuxE, 
               
               
                   
                   
                 NpFAD, EcTesA′ 
               
               
                 pAlkane6 
                 pYX212 
                 AnnpgA, NpFAD, MmCAR, 
               
               
                   
                   
                 HFA1, ETR1 
               
               
                 pISP08 
                 pSPGM1 
                 fldA,fpr 
               
               
                 pYX212 
                   
                 ampR, URA3, pYX212t, TPIp 
               
               
                 p423GPD 
                   
                 ampR, HIS3, TDH3p, CYC1t 
                 ATCC 87355 
               
               
                 pSP-A 
                 pSPGM2 
                 ACB1 
               
               
                 pScACC1 
                 p423GPD 
                 ACC1 
               
               
                 pRtACC1 
                 p423GPD 
                 RtACC1 
               
               
                 pAlcohol1 
                 pYX212 
                 FaCoAR 
               
               
                 pAlkane9 
                 pYX212 
                 PlLuxD, PlLuxC, PlLuxE, 
               
               
                   
                   
                 SeFAD, EcTesA′ 
               
               
                 pFAR 
                 pYX212 
                 SeFAR 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Oligonucleotide primers. 
               
             
          
           
               
                   
                   
                   
                 SEQ 
               
               
                 PR ID 
                 Name 
                 Sequence (5′→3′) 
                 ID NO 
               
               
                   
               
               
                   1 
                 ARE1- 
                 TGTGTTTCCGTACCGCAC 
                   1 
               
               
                   
                 UP-f 
                   
                   
               
               
                   
               
               
                   2 
                 ARE1- 
                 CAGCGTACGAAGCTTCAGCTGCGGAATTGAGTCTGC 
                   2 
               
               
                   
                 UP-r 
                   
                   
               
               
                   
               
               
                   3 
                 ARE1- 
                 GTGATATCAGATCCACTAGGCAACACCAAGTTTCTACG 
                   3 
               
               
                   
                 DW-f 
                 G 
                   
               
               
                   
               
               
                   4 
                 ARE1- 
                 ATTTTTGTCACCTGCAAACTC 
                   4 
               
               
                   
                 DW-r 
                   
                   
               
               
                   
               
               
                   5 
                 kanMX- 
                 CTGAAGCTTCGTACGCTG 
                   5 
               
               
                   
                 1-f 
                   
                   
               
               
                   
               
               
                   6 
                 kanMX- 
                 TCACCATGAGTGACGACTGA 
                   6 
               
               
                   
                 1-r 
                   
                   
               
               
                   
               
               
                   7 
                 kanMX- 
                 TTCCAACATGGATGCTGAT 
                   7 
               
               
                   
                 2-f 
                   
                   
               
               
                   
               
               
                   8 
                 kanMX- 
                 CTAGTGGATCTGATATCAC 
                   8 
               
               
                   
                 2-r 
                   
                   
               
               
                   
               
               
                   9 
                 ARE2- 
                 CTCGTCGGTTTATCTGCC 
                   9 
               
               
                   
                 UP-f 
                   
                   
               
               
                   
               
               
                  10 
                 ARE2- 
                 CAGCGTACGAAGCTTCAGCGTTGAGCTTTTGGATGC 
                  10 
               
               
                   
                 UP-r 
                   
                   
               
               
                   
               
               
                  11 
                 ARE2- 
                 GTGATATCAGATCCACTAGGCTCGGTATCTGCATGGG 
                  11 
               
               
                   
                 DW-f 
                   
                   
               
               
                   
               
               
                  12 
                 ARE2- 
                 GCACGATATGAATAGCAGTGG 
                  12 
               
               
                   
                 DW-r 
                   
                   
               
               
                   
               
               
                  13 
                 DGA1- 
                 CGTTATTGTAACTGGTAATCAGAG 
                  13 
               
               
                   
                 UP-f 
                   
                   
               
               
                   
               
               
                  14 
                 DGA1- 
                 CAGCGTACGAAGCTTCAGCCTTTCGGTAATACCGGC 
                  14 
               
               
                   
                 UP-r 
                   
                   
               
               
                   
               
               
                  15 
                 DGA1- 
                 GTGATATCAGATCCACTAGAATGTTGTTGTTGGAAGGC 
                  15 
               
               
                   
                 DW-f 
                   
                   
               
               
                   
               
               
                  16 
                 DGA1- 
                 GCTTTCCTAAACTTACATTCAAA 
                  16 
               
               
                   
                 DW-r 
                   
                   
               
               
                   
               
               
                  17 
                 LRO1- 
                 CTCCTTTGTACTTCTTTGTTCC 
                  17 
               
               
                   
                 UP-f 
                   
                   
               
               
                   
               
               
                  18 
                 LRO1- 
                 CAGCGTACGAAGCTTCAGCCTGTTGATGATGAATGTGG 
                  18 
               
               
                   
                 UP-r 
                   
                   
               
               
                   
               
               
                  19 
                 LRO1- 
                 GTGATATCAGATCCACTAGCAAGCGGTAATGGCGATC 
                  19 
               
               
                   
                 DW-f 
                   
                   
               
               
                   
               
               
                  20 
                 LRO1- 
                 CGGTTGTTTTTCCTCTATGC 
                  20 
               
               
                   
                 DW-r 
                   
                   
               
               
                   
               
               
                  21 
                 POX1- 
                 GCCCTATATTTACGGTATTAGTTG 
                  21 
               
               
                   
                 UP-f 
                   
                   
               
               
                   
               
               
                  22 
                 POX1- 
                 CAGCGTACGAAGCTTCAGGGGATTAATAGTAGTACGTC 
                  22 
               
               
                   
                 UP-r 
                 TCGT 
                   
               
               
                   
               
               
                  23 
                 POX1- 
                 GTGATATCAGATCCACTAGCAGATGGGGCAGGGAAG 
                  23 
               
               
                   
                 DW-f 
                   
                   
               
               
                   
               
               
                  24 
                 POX1- 
                 GTAGTCATGTCATTGATTCGTCA 
                  24 
               
               
                   
                 DW-r 
                   
                   
               
               
                   
               
               
                  25 
                 ACB1-f 
                 AGTTTTAATTACAAGGATCCACTATGGTTTCCCAATTAT 
                  25 
               
               
                   
                   
                 TCG 
                   
               
               
                   
               
               
                  26 
                 ACB1-r 
                 GCGGATCTTAGCTAGCCGCGGTACCCTAAGAGGAGTAC 
                  26 
               
               
                   
                   
                 TTGGCA 
                   
               
               
                   
               
               
                  69 
                 P PGK1 - 
                 AACTTAGAUTAGATTGCTATGCTTTC 
                  27 
               
               
                   
                 P TEF1 -fW 
                   
                   
               
               
                   
               
               
                  70 
                 P PGK1 - 
                 ATTTGTTGUAAAAAGTAGATAATTACTTCC 
                  28 
               
               
                   
                 P TEF1 -rev 
                   
                   
               
               
                   
               
               
                  71 
                 P PGKl -fw 
                 CGTGCGAUGGAAGTACCTTCAAAGAATGG 
                  29 
               
               
                   
               
               
                  72 
                 yqeF-fw 
                 ACAACAAAUATAAAACAATGAAGGATGTCGTAATCGT 
                  30 
               
               
                   
                   
                 TG 
                   
               
               
                   
               
               
                  73 
                 yqeF-rev 
                 CACGCGAUTTATTCGTCTCTTTCGATAGTCAATG 
                  31 
               
               
                   
               
               
                  74 
                 fadA-fw 
                 CGTGCGAUTTAGACTCTTTCAAATACAGTAGCG 
                  32 
               
               
                   
               
               
                  75 
                 fadA-rev 
                 ATCTAAGTUTTAATAAAACAATGGAACAAGTAGTAATC 
                  33 
               
               
                   
                   
                 GTAGAC 
                   
               
               
                   
               
               
                  76 
                 fadB-fw 
                 ACAACAAAUATAAAACAATGTTGTATAAAGGTGACAC 
                  34 
               
               
                   
                   
                 ATTGTAC 
                   
               
               
                   
               
               
                  77 
                 fadB-rev 
                 CACGCGAUTTAGGCAGTTTTCAAGTCACC 
                  35 
               
               
                   
               
               
                  78 
                 tdTER- 
                 CGTGCGAUTTAGATTCTATCGAATCTTTCGAC 
                  36 
               
               
                   
                 fw 
                   
                   
               
               
                   
               
               
                  79 
                 tdTER- 
                 ATCTAAGTUTTAATAAAACAATGATAGTAAAGCCAATG 
                  37 
               
               
                   
                 rev 
                 GTAAGG 
                   
               
               
                   
               
               
                  80 
                 FOX3c- 
                 CGTGCGAUCTATTCTTTAATAAAGATGGCGG 
                  38 
               
               
                   
                 fw 
                   
                   
               
               
                   
               
               
                  81 
                 FOX3c- 
                 ATCTAAGTUTTAATAAAACAATGGGTAAGGGTGAATC 
                  39 
               
               
                   
                 rev 
                 GAAG 
                   
               
               
                   
               
               
                  82 
                 FOX2c- 
                 ACAACAAAUATAAAACAATGCCTGGAAATTTATCCTTC 
                  40 
               
               
                   
                 fw 
                   
                   
               
               
                   
               
               
                  83 
                 FOX2c- 
                 CACGCGAUTTATTTTGCCTGCGATAGTTTTAC 
                  41 
               
               
                   
                 rev 
                   
                   
               
               
                   
               
               
                  84 
                 ERG10- 
                 ACAACAAAUATAAAACAATGTCTCAGAACGTTTACATT 
                  42 
               
               
                   
                 fw 
                 GTATC 
                   
               
               
                   
               
               
                  85 
                 ERG10- 
                 CACGCGAUTCATATCTTTTCAATGACAATAGAGG 
                  43 
               
               
                   
                 rev 
                   
                   
               
               
                   
               
               
                  86 
                 TES1c- 
                 ACAACAAAUATAAAACAATGAGTGCTTCCAAAATGGC 
                  44 
               
               
                   
                 fw 
                 CATG 
                   
               
               
                   
               
               
                  87 
                 TES1c- 
                 CACGCGAUTCATCGAATGTCTCGTTCTGACC 
                  45 
               
               
                   
                 rev 
                   
                   
               
               
                   
               
               
                  88 
                 tesA-fw 
                 ACAACAAAUATAAAACAATGGCCGATACTTTGTTAATT 
                  46 
               
               
                   
                   
                 TTG 
                   
               
               
                   
               
               
                  89 
                 tesA-rev 
                 CACGCGAUTCAAGAATCGTGATTGACTAATGG 
                  47 
               
               
                   
               
               
                  90 
                 tesB-fw 
                 ACAACAAAUATAAAACAATGTCTCAAGCTTTGAAGAA 
                  48 
               
               
                   
                   
                 CTTG 
                   
               
               
                   
               
               
                  91 
                 tesB-rev 
                 CACGCGAUTCAGTTGTGGTTTCTCATAACACC 
                  49 
               
               
                   
               
               
                  92 
                 fadM-fw 
                 ACAACAAAUATAAAACAATGCAAACTCAAATCAAGGT 
                  50 
               
               
                   
                   
                 TAGA 
                   
               
               
                   
               
               
                  93 
                 fadM-rev 
                 CACGCGAUTCACTTAACCATTTGTTCCAACTT 
                  51 
               
               
                   
               
               
                  94 
                 yciA-fw 
                 ACAACAAAUATAAAACAATGTCTACTACTCACAACGTT 
                  52 
               
               
                   
                   
                 CCA 
                   
               
               
                   
               
               
                  95 
                 yciA-rev 
                 CACGCGAUTCATTCAACTGGCAAAGCTCTTGG 
                  53 
               
               
                   
               
               
                 104 
                 Acr1-F1 
                 GCATAGCAATCTAATCTAAGTTTTAATTACAAAATGAA 
                  54 
               
               
                   
                   
                 TAAGAAGTTGGAAGC 
                   
               
               
                   
               
               
                 105 
                 Acr1-R1 
                 GGATACCCGGGTCGACGCGTAAGCTTGTGGGCCCTATC 
                  55 
               
               
                   
                   
                 ACCAATGTTCACCAGGG 
                   
               
               
                   
               
               
                 106 
                 FAcoAR 
                 GCATAGCAATCTAATCTAAGTTTTAATTACAAAATGAA 
                  56 
               
               
                   
                 1-F 
                 TTATTTCTTGACAGGTG 
                   
               
               
                   
               
               
                 107 
                 FAcoAR 
                 GGATACCCGGGTCGACGCGTAAGCTTGTGGGCCCTATT 
                  57 
               
               
                   
                 1-R 
                 ACCAATAGATACCTCTCA 
                   
               
               
                   
               
               
                 108 
                 npgA-F2 
                 GGAAGTAATTATCTACTTTTTACAACAAATATAACAAA 
                  58 
               
               
                   
                   
                 ATGGTGCAAGACACATCAAG 
                   
               
               
                   
               
               
                 109 
                 npgA-R2 
                 GACATAACTAATTACATGACTCGAGGTCGACGGTATCT 
                  59 
               
               
                   
                   
                 TAGGATAGGCAATTACACAC 
                   
               
               
                   
               
               
                 110 
                 SynaaC- 
                 GGAAGTAATTATCTACTTTTTACAACAAATATAACAAA 
                  60 
               
               
                   
                 F 
                 ATGGACTCAGGTCACGGTGC 
                   
               
               
                   
               
               
                 111 
                 SynaaC- 
                 GACATAACTAATTACATGACTCGAGGTCGACGGTATCT 
                  61 
               
               
                   
                 R 
                 CAGAACATTTCGTCTATCAAG 
                   
               
               
                   
               
               
                 112 
                 CYP4G2- 
                 CTCATTAAAAAACTATATCAATTAATTTGAATTAACTT 
                  62 
               
               
                   
                 R 
                 ACATTGCCTTCATTGCTTC 
                   
               
               
                   
               
               
                 113 
                 CYP4G2- 
                 GAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAA 
                  63 
               
               
                   
                 F 
                 TGGACTCCGCCAACAACTC 
                   
               
               
                   
               
               
                 114 
                 MmCAR- 
                 CAAGAACTTAGTTTCGAATAAACACACATAAACAAAC 
                  64 
               
               
                   
                 F1 
                 AAAATGTCACCTATCACCAGAGAAG 
                   
               
               
                   
               
               
                 115 
                 MmCAR- 
                 CTTATTTAATAATAAAAATCATAAATCATAAGAAATTC 
                  65 
               
               
                   
                 R1 
                 GCTTACAACAAACCCAACAATCTC 
                   
               
               
                   
               
               
                 116 
                 RtFAS1- 
                 CTATAACTACAAAAAACACATACATAAACTAAAAATG 
                  66 
               
               
                   
                 F 
                 AACGGCCGAGCGACGCGGAG 
                   
               
               
                   
               
               
                 117 
                 RtFAS1- 
                 CTCATTAAAAAACTATATCAATTAATTTGAATTAACTC 
                  67 
               
               
                   
                 R 
                 AGAGCCCGCCGAAGACGTCGAG 
                   
               
               
                   
               
               
                 118 
                 RtFAS2- 
                 GACATAACTAATTACATGACTCGAGGTCGACGGTATCC 
                  68 
               
               
                   
                 R 
                 TACTTCTGGGCGATGACGACGG 
                   
               
               
                   
               
               
                 119 
                 RtFAS2- 
                 GAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAA 
                  69 
               
               
                   
                 F 
                 TGGTCGCGGCGCAGGACTTGC 
                   
               
               
                   
               
               
                 120 
                 RtACC1- 
                 CAAGAACTTAGTTTCGAATAAACACACATAAACAAAC 
                  70 
               
               
                   
                 F 
                 AAAATGCCATTCTCTGGCGAGGCGAAG 
                   
               
               
                   
               
               
                 121 
                 RtACC1- 
                 GGATACCCGGGTCGACGCGTAAGCTTGTGGGCCCTACT 
                  71 
               
               
                   
                 R 
                 AGGCGAGGATGCGGGCGAGG 
                   
               
               
                   
               
               
                 122 
                 hfd1(up)- 
                 GATTATCAATGTCCCAGTTATACG 
                  72 
               
               
                   
                 F 
                   
                   
               
               
                   
               
               
                 123 
                 hfd1(up)- 
                 TAAGTTTGGTCGTTTCATTCAG 
                  73 
               
               
                   
                 R 
                   
                   
               
               
                   
               
               
                 124 
                 hfd(dn)- 
                 GAGTACGAGGATCTTGATGAGAC 
                  74 
               
               
                   
                 F 
                   
                   
               
               
                   
               
               
                 125 
                 hfd(dn)R 
                 CACTTGTTATTGCCATTTCTGTC 
                  75 
               
               
                   
               
               
                 126 
                 hfd1(up)- 
                 CGAAAGGTTACTTATACATCAAATAATTAATTAACCTT 
                  76 
               
               
                   
                 URA3-R 
                 AAACATTACGTTCACATGTTGGTGATAAATTACTATG 
                   
               
               
                   
               
               
                 127 
                 URA3 
                 GGTTAATTAATTATTTGATGTATAAGTAACCTTTCGTTT 
                  77 
               
               
                   
                 (hfd1)-F 
                 AAAAATTTCATATGGGCGATAATATATCGTGATTCTGG 
                   
               
               
                   
                   
                 GTAGAAGATCG 
                   
               
               
                   
               
               
                 128 
                 URA3 
                 CTATTATCTTGTTAATGGTCTCATCAAGATCCTCGTACT 
                  78 
               
               
                   
                 (hfd1)-R 
                 CCATCGATAAGCTTGATATCG 
                   
               
               
                   
               
               
                 129 
                 Pox1(up)- 
                 GATTCCTTCAGTTCCACTTTTTGC 
                  79 
               
               
                   
                 F 
                   
                   
               
               
                   
               
               
                 130 
                 Pox1(up)- 
                 GTAGCATCGTAATAGTCCGTGTC 
                  80 
               
               
                   
                 R 
                   
                   
               
               
                   
               
               
                 131 
                 Pox1(dn)- 
                 GATCTCTAAAGTTGTGCAGCCAC 
                  81 
               
               
                   
                 F 
                   
                   
               
               
                   
               
               
                 132 
                 Pox1(dn)- 
                 CGCATTAGCTGCACCACCTAAC 
                  82 
               
               
                   
                 R 
                   
                   
               
               
                   
               
               
                 133 
                 Pox1(up)- 
                 GAATTGAAACAAAAGTCGCAAAACAGAGGGTTCGAAG 
                  83 
               
               
                   
                 UAR3-R 
                 GAAAACAGGAAACCTCTACTCACATATCGCAATACTAA 
                   
               
               
                   
                   
                 TTTATTAT 
                   
               
               
                   
               
               
                 134 
                 URA3 
                 CTTCGAACCCTCTGTTTTGCGACTTTTGTTTCAATTCAA 
                  84 
               
               
                   
                 (pox1)-F 
                 CTAGTGTCGCCAAGTTTTAACGTGATTCTGGGTAGAAG 
                   
               
               
                   
                   
                 ATCG 
                   
               
               
                   
               
               
                 135 
                 URA3 
                 GAGCCAATAGTTGTGGCTGCACAACTTTAGAGATCCAT 
                  85 
               
               
                   
                 (pox1)-R 
                 CGATAAGCTTGATATCG 
                   
               
               
                   
               
               
                 136 
                 FAA1 
                 CACCCACCCATCGCATATCAGG 
                  86 
               
               
                   
                 (up)-F 
                   
                   
               
               
                   
               
               
                 137 
                 FAA1 
                 CTTAACATCCCTCCAACCCATAGC 
                  87 
               
               
                   
                 (up)-R 
                   
                   
               
               
                   
               
               
                 138 
                 FAA1 
                 GAAATTAGAGTCCGTTTACAGATC 
                  88 
               
               
                   
                 (dn)-F 
                   
                   
               
               
                   
               
               
                 139 
                 FAA1 
                 GTCAAAGAACACTATGCCTGCTAG 
                  89 
               
               
                   
                 (dn)-R 
                   
                   
               
               
                   
               
               
                 140 
                 FAA1 
                 CTGAAAAAGTGCTTTAGTATGATGAGGCTTTCCTATCA 
                  90 
               
               
                   
                 (up)- 
                 TGGAAATGTTGATCCATTACATATTGTTGTCTTTTTTTG 
                   
               
               
                   
                 URA3-R 
                 TC 
                   
               
               
                   
               
               
                 141 
                 URA3 
                 GATAGGAAAGCCTCATCATACTAAAGCACTTTTTCAGT 
                  91 
               
               
                   
                 (FAA1)-F 
                 TTTTTGCTTTAGAACTGCTACCGTGATTCTGGGTAGAA 
                   
               
               
                   
                   
                 GATCG 
                   
               
               
                   
               
               
                 142 
                 URA3 
                 CAACATATTCGTTAGATCTGTAAACGGACTCTAATTTC 
                  92 
               
               
                   
                 (FAA1)-R 
                 CATCGATAAGCTTGATATCG 
                   
               
               
                   
               
               
                 143 
                 FAA4 
                 GTCCCCATCAATTAAGAACCCTC 
                  93 
               
               
                   
                 (up)-F 
                   
                   
               
               
                   
               
               
                 144 
                 FAA4 
                 GATGCTGAGGAGTTTATGGGTC 
                  94 
               
               
                   
                 (up)-R 
                   
                   
               
               
                   
               
               
                 145 
                 FAA4 
                 CCTTTACCGATGATGGCTGGTTC 
                  95 
               
               
                   
                 (dn)-F 
                   
                   
               
               
                   
               
               
                 146 
                 FAA4 
                 GATGTAACAAGACCGTTTTCTGGAG 
                  96 
               
               
                   
                 (dn)-R 
                   
                   
               
               
                   
               
               
                 147 
                 FAA4 
                 GAAAATGAAACGTAGTGTTTATGAAGGGCAGGGGGGA 
                  97 
               
               
                   
                 (up)- 
                 AAGTAAAAAACTATGTCTTCCTTTACATTTTGATGCGT 
                   
               
               
                   
                 URA3-R 
                 ACTTCTTAG 
                   
               
               
                   
               
               
                 148 
                 URA3 
                 CTTTCCCCCCTGCCCTTCATAAACACTACGTTTCATTTT 
                  98 
               
               
                   
                 (FAA4)-F 
                 CTAAGAGCATCAATTTGCGTGATTCTGGGTAGAAGATCG 
                   
               
               
                   
               
               
                 149 
                 URA3 
                 GATATCACCGGTACGGAACCAGCCATCATCGGTAAAG 
                  99 
               
               
                   
                 (FAA4)-R 
                 GCATCGATAAGCTTGATATCG 
                   
               
               
                   
               
               
                 150 
                 Orf1594- 
                 GGATCCAAAACAATGTTCGG 
                 100 
               
               
                   
                 CP FW 
                   
                   
               
               
                   
               
               
                 151 
                 Orf1594- 
                 GATTGCTAAGGCTAAAGGTTGG 
                 101 
               
               
                   
                 CP RV 
                   
                   
               
               
                   
               
               
                 152 
                 Acr1-CP 
                 GCTTTAATCACTGGTGCCTC 
                 102 
               
               
                   
                 FW 
                   
                   
               
               
                   
               
               
                 153 
                 Acr1-CP 
                 TTCACCAATGTTCACCAGG 
                 103 
               
               
                   
                 RV 
                   
                   
               
               
                   
               
               
                 154 
                 Orf1593-  
                 GCCACAATTAGAAGCCTCCTTAG 
                 104 
               
               
                   
                 CP FW 
                   
                   
               
               
                   
               
               
                 155 
                 Orf1593- 
                 CTGCTGCCAAACCGTATGC 
                 105 
               
               
                   
                 CP RV 
                   
                   
               
               
                   
               
               
                 156 
                 NpFAD- 
                 GCCTACTCCAGAATCAACGC 
                 106 
               
               
                   
                 CP FW 
                   
                   
               
               
                   
               
               
                 157 
                 NpFAD- 
                 GCCTTACTCTCTGCGAAGTG 
                 107 
               
               
                   
                 CP RV 
                   
                   
               
               
                   
               
               
                 158 
                 Fdx FW 
                 ATCGAAGCGGCCGCAAAACAATGCCAAAGATTGTTATT 
                 108 
               
               
                   
                   
                 TTGC 
                   
               
               
                   
               
               
                 159 
                 Fdx RV 
                 ATCGTCGAGCTCTTAATGCTCACGCGCATG 
                 109 
               
               
                   
               
               
                 160 
                 Fpr FW 
                 ATGGCTGATTGGGTAACAGG 
                 110 
               
               
                   
               
               
                 161 
                 Fpr RV 
                 ACAGCGGAGCATTACTGGTAA 
                 111 
               
               
                   
               
               
                 162 
                 Fdx M 
                 ATCGAAGCGGCCGCAAAACAATGCTTTCTCTTCGTCAA 
                 112 
               
               
                   
                 FW 
                 TCTATTCGTTTTTTTAAACGTTCTGGTATTATGCCAAAG 
                   
               
               
                   
                   
                 ATTGTTATTTTGC 
                   
               
               
                   
               
               
                 163 
                 Fpr M 
                 CATTATCCCGGGAAAACAATGCTTTCTCTTCGTCAATCT 
                 113 
               
               
                   
                 FW 
                 ATTCGTTTTTTTAAACGTTCTGGTATTATGGCTGATTGG 
                   
               
               
                   
                   
                 GTAACAGG 
                   
               
               
                   
               
               
                 164 
                 Fpr M 
                 CATTATCTCGAGTTACCAGTAATGCTCCGCTGT 
                 114 
               
               
                   
                 RV 
                   
                   
               
               
                   
               
               
                 165 
                 npgA 
                 AACTACAAAAAACACATACATAAACTAAAAATGCTTTC 
                 115 
               
               
                   
                 FW 
                 TCTTCGTCAATCTATTCGTTTTTTTAAACGTTCTGGTAT 
                   
               
               
                   
                   
                 TATGGTGCAAGACACATCAAGCG 
                   
               
               
                   
               
               
                 166 
                 npgA 
                 AAAAAACTATATCAATTAATTTGAATTAACTTAGGATA 
                 116 
               
               
                   
                 RV 
                 GGCAATTACACACCCCA 
                   
               
               
                   
               
               
                 167 
                 NPFAD 
                 GTTTCGAATAAACACACATAAACAAACAAAATGCTTTC 
                 117 
               
               
                   
                 FW 
                 TCTTCGTCAATCTATTCGTTTTTTTAAACGTTCTGGTAT 
                   
               
               
                   
                   
                 TATGCAACAATTAACAGACCAATCAAAGG 
                   
               
               
                   
               
               
                 168 
                 NPFAD 
                 CTAATTACATGACTCGAGGTCGACGGTATCTCAAGCAC 
                 118 
               
               
                   
                 RV 
                 CTATCAAACCGTAAGCAC 
                   
               
               
                   
               
               
                 169 
                 MmCAR 
                 ACAAAAAGTTTTTTTAATTTTAATCAAAAAATGCTTTCT 
                 119 
               
               
                   
                 FW 
                 CTTCGTCAATCTATTCGTTTTTTTAAACGTTCTGGTATT 
                   
               
               
                   
                   
                 ATGTCACCTATCACCAGAGAAGAAAG 
                   
               
               
                   
               
               
                 170 
                 MmCAR 
                 AAATCATTAAAGTAACTTAAGGAGTTAAATTTACAACA 
                 120 
               
               
                   
                 RV 
                 AACCCAACAATCTCAAA 
                   
               
               
                   
               
               
                 171 
                 ETR1 
                 TAGCAATCTAATCTAAGTTTTAATTACAAAATGCTTCC 
                 121 
               
               
                   
                 FW 
                 CACATTCAAACGTTACATG 
                   
               
               
                   
               
               
                 172 
                 ETR1 
                 GGGTCGACGCGTAAGCTTGTGGGCCCTATTACCATTCT 
                 122 
               
               
                   
                 RV 
                 AAAACAACCATTTTTTTCTTCC 
                   
               
               
                   
               
               
                 173 
                 HFA1 
                 TTATCTACTTTTTACAACAAATATAACAAAATGAGATC 
                 123 
               
               
                   
                 FW 
                 TATAAGAAAATGGGCGTACG 
                   
               
               
                   
               
               
                 174 
                 HFA1b 
                 TTGGTCCGAAGTGGTGATCACG 
                 124 
               
               
                   
                 FW 
                   
                   
               
               
                   
               
               
                 175 
                 HFA1b 
                 GATCATGTTACGCCCTTCAGGATATTC 
                 125 
               
               
                   
                 RV 
                   
                   
               
               
                   
               
               
                 176 
                 HFA1a 
                 GCAGGAAAAGAAACAGATTTCTTGACTAG 
                 126 
               
               
                   
                 RV 
                   
                   
               
               
                   
               
               
                 177 
                 HFA1c 
                 CAGTACATCGTCTCGAGGAAATTGTG 
                 127 
               
               
                   
                 FW 
                   
                   
               
               
                   
               
               
                 178 
                 HFA1 
                 AATAAAAATCATAAATCATAAGAAATTCGCCTATCTCT 
                 128 
               
               
                   
                 RV 
                 TTCGCTTACTGTCCACCAAC 
                   
               
               
                   
               
               
                 187 
                 PGK1 
                 GGGGTGGTTTAGTTTAGTAGAA 
                 129 
               
               
                   
                 SEQ 
                   
                   
               
               
                   
               
               
                 188 
                 ADH1 
                 GCAACCTGACCTACAGGAAAGA 
                 130 
               
               
                   
                 SEQ 
                   
                   
               
               
                   
               
               
                 189 
                 TEF1 
                 TTTTACTTCTTGCTCATTAGAAAG 
                 131 
               
               
                   
                 SEQ 
                   
                   
               
               
                   
               
               
                 190 
                 CYC1 
                 GGACCTAGACTTCAGGTTGTC 
                 132 
               
               
                   
                 SEQ 
                   
                   
               
               
                   
               
               
                 192 
                 MdCPR- 
                 GTGACATAACTAATTACATGACTCGAGGTCGACGGTAT 
                 133 
               
               
                   
                 R 
                 CTTAACTCCAAACATCAGCGGAG 
                   
               
               
                   
               
               
                 193 
                 MdCPR- 
                 CAAGAACTTAGTTTCGAATAAACACACATAAACAAAC 
                 134 
               
               
                   
                 F 
                 AAAATGAGTGCCGAACACGTTGAAG 
                   
               
               
                   
               
               
                 194 
                 Orf1594- 
                 CAAGAACTTAGTTTCGAATAAACACACATAAACAAAC 
                 135 
               
               
                   
                 F 
                 AAAATGTTCGGTTTAATAGGTC 
                   
               
               
                   
               
               
                 195 
                 Orf1594- 
                 CTTATTTAATAATAAAAATCATAAATCATAAGAAATTC 
                 136 
               
               
                   
                 R 
                 GCTCAGATTGCTAAGGCTAAAG 
                   
               
               
                   
               
               
                 196 
                 P450G2- 
                 CTCATTAAAAAACTATATCAATTAATTTGAATTAACTT 
                 137 
               
               
                   
                 R 
                 ACATTGCCTTCATTGCTTC 
                   
               
               
                   
               
               
                 197 
                 P450G2- 
                 GAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAA 
                 138 
               
               
                   
                 F 
                 TGGACTCCGCCAACAACTC 
                   
               
               
                   
               
               
                 198 
                 TPIp-F2 
                 GAGTAAAAAAGGAGTAGAAACATTTTGAAGCTATGTTT 
                 139 
               
               
                   
                   
                 AAAGATTACGGATATTTAAC 
                   
               
               
                   
               
               
                 199 
                 TPIp-R2 
                 GCTTCTTCGACGAGGGTTCCATTTTTAGTTTATGTATGT 
                 140 
               
               
                   
                   
                 GTTTTTTG 
                   
               
               
                   
               
               
                 200 
                 TDH2t- 
                 CAAATGCCTATTGTGCAGATGTTATAATATCTGTGCGT 
                 141 
               
               
                   
                 R2 
                 GCGAAAAGCCAATTAGTGTG 
                   
               
               
                   
               
               
                 201 
                 Acr1-F2 
                 CAAGAACTTAGTTTCGAATAAACACACATAAACAAAC 
                 142 
               
               
                   
                   
                 AAAATGAATAAGAAGTTGGAAG 
                   
               
               
                   
               
               
                 202 
                 Acr1-R2 
                 CTTATTTAATAATAAAAATCATAAATCATAAGAAATTC 
                 143 
               
               
                   
                   
                 GCTCACCAATGTTCACCAGGG 
                   
               
               
                   
               
               
                 203 
                 SmCPR- 
                 GACATAACTAATTACATGACTCGAGGTCGACGGTATCT 
                 144 
               
               
                   
                 R2 
                 TACCATACATCGCGCAAGTAC 
                   
               
               
                   
               
               
                 206 
                 FaCoAR1 
                 GCTTAAATCTATAACTACAAAAAACACATACATAAACT 
                 145 
               
               
                   
                 (pYX)-F 
                 AAAAATGAATTATTTCTTGACAGGTGG 
                   
               
               
                   
               
               
                 207 
                 FaCoAR1 
                 CGGATACCCGGGTCGACGCGTAAGCTTGTGGGCCCTAT 
                 146 
               
               
                   
                 (pYX)-R 
                 TACCAATAGATACCTCTCATAATGG 
                   
               
               
                   
               
               
                 208 
                 SeFAR- 
                 CTATAACTACAAAAAACACATACATAAACTAAAAATG 
                 147 
               
               
                   
                 F2 
                 TTCGGTTTAATAGGTCAC 
                   
               
               
                   
               
               
                 209 
                 SeFAR- 
                 CTCATTAAAAAACTATATCAATTAATTTGAATTAACTC 
                 148 
               
               
                   
                 R2 
                 AGATTGCTAAGGCTAAAG 
                   
               
               
                   
               
               
                 210 
                 SeADO- 
                 CAAGAACTTAGTTTCGAATAAACACACATAAACAAAC 
                 149 
               
               
                   
                 F1 
                 AAAATGCCACAATTAGAAGCCTC 
                   
               
               
                   
               
               
                 211 
                 SeADO- 
                 CTTATTTAATAATAAAAATCATAAATCATAAGAAATTC 
                 150 
               
               
                   
                 R1 
                 GCTTAGACTGCTGCCAAACCGTATG 
                   
               
               
                   
               
               
                 212 
                 EcFd-F1 
                 GAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAA 
                 151 
               
               
                   
                   
                 TGCCAAAGATTGTTATTTTG 
                   
               
               
                   
               
               
                 213 
                 EcFd-R1 
                 CTAAATCATTAAAGTAACTTAAGGAGTTAAATTTAATG 
                 152 
               
               
                   
                   
                 CTCACGCGCATGGTTG 
                   
               
               
                   
               
               
                 214 
                 EcFNR- 
                 GACATAACTAATTACATGACTCGAGGTCGACGGTATCT 
                 153 
               
               
                   
                 F1 
                 TACCAGTAATGCTCCGCTG 
                   
               
               
                   
               
               
                 215 
                 EcFNR- 
                 GGAAGTAATTATCTACTTTTTACAACAAATATAACAAA 
                 154 
               
               
                   
                 R1 
                 ATGGCTGATTGGGTAACAGG 
                   
               
               
                   
               
               
                 216 
                 SeFAD 
                 TTATCTACTTTTTACAACAAATATAACAAAATGCCACA 
                   
               
               
                   
                 FW 
                 ATTAGAAGCCTCCTTAGAAT 
                   
               
               
                   
               
               
                 217 
                 SeFAD 
                 AATAAAAATCATAAATCATAAGAAATTCGCTTAGACTG 
                   
               
               
                   
                 RV 
                 CTGCCAAACCGTATGC 
                   
               
               
                   
               
               
                 218 
                 NpFAD 
                 AATAAAAATCATAAATCATAAGAAATTCGCTCAAGCA 
                   
               
               
                   
                 RV 
                 CCTATCAAACCGTAAGCAC 
                   
               
               
                   
               
               
                 219 
                 TesA 
                 TAGCAATCTAATCTAAGTTTTAATTACAAAATGGCCGA 
                   
               
               
                   
                 FW 
                 TACTTTGTTAATTTTGG 
                   
               
               
                   
               
               
                 220 
                 TesA RV 
                 CCGGGTCGACGCGTAAGCTTGTGGGCCCTATCAAGAAT 
                   
               
               
                   
                   
                 CGTGATTGACTAATGGTTG 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Polypeptide sequences. 
               
             
          
           
               
                   
                 Gene  
                   
                 SEQ ID 
               
               
                 P ID 
                 name 
                 Sequence 
                 NO 
               
               
                   
               
               
                 1 
                 CnFatB1 
                 MVASVAASAFFPTPSFSSTASAKASKTIGEGSESLDVRGIVAK 
                 155 
               
               
                   
                   
                 PTSSSAAMQGKVKAQAVPKINGTKVGLKTESQKAEEDAAPSS 
                   
               
               
                   
                   
                 APRTFYNQLPDWSVLLAAVTTIFLAAEKQWTLLDWKPRRPD 
                   
               
               
                   
                   
                 MLTDAFSLGKIVQDGLIFRQNFSIRSYEIGADRTASIETLMNHL 
                   
               
               
                   
                   
                 QETALNHVRNAGLLGDGFGATPEMSKRNLIWVVTKMQVLV 
                   
               
               
                   
                   
                 EHYPSWGDVVEVDTWVGASGKNGMRRDWHVRDYRTGQTI 
                   
               
               
                   
                   
                 LRATSVWVMMNKHTRKLSKMPEEVRAEIGPYFVEHAAIVDE 
                   
               
               
                   
                   
                 DSRKLPKLDDDTADYIKWGLTPRWSDLDVNQHVNNVKYIG 
                   
               
               
                   
                   
                 WILESAPISILENHELASMTLEYRRECGRDSVLQSLTAISNDCT 
                   
               
               
                   
                   
                 GGLPEASIECQHLLQLECGAEIVRGRTQWRPRRASGPTSAGSA 
                   
               
               
                   
               
               
                 2 
                 AbTesA 
                 MAKTILILGDSLSAGYGINPEQGWVALLQKRLDQQFPKQHKV 
                 156 
               
               
                   
                   
                 INASVSGETTSGALARLPKLLTTYRPNVVVIELGGNDALRGQP 
                   
               
               
                   
                   
                 PQMIQSNLEKLIQHSQKAKSKVVVFGMKIPPNYGTAYSQAFE 
                   
               
               
                   
                   
                 NNYKVVSQTYQVKLLPFFLDGVAGHKSLMQNDQIHPNAKAQ 
                   
               
               
                   
                   
                 SILLNNAYPYIKGAL 
                   
               
               
                   
               
               
                 3 
                 P1LuxC 
                 MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNNSHVK 
                 157 
               
               
                   
                   
                 NIIDYNENNKLRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDL 
                   
               
               
                   
                   
                 KKYMGYSEAMAKLEANWISMILCSKGGLYDVVENELGSRHI 
                   
               
               
                   
                   
                 MDEWLPQDESYIKAFPKGKSIHLLAGNVPLSGIMSILRAILTK 
                   
               
               
                   
                   
                 NQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVVYWPHQG 
                   
               
               
                   
                   
                 DTSLAKEIMQHMDVIVAWGGEDAINWAVEHAPPYADVIKFG 
                   
               
               
                   
                   
                 SKKSFCIIDNPVDLTSAATGAAHDICFYDQRACFSAQNIYYMG 
                   
               
               
                   
                   
                 NQYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL 
                   
               
               
                   
                   
                 FAGLKVEVDVHQRWMIIESNAGVEFNQPLGRCVYLHHVDNI 
                   
               
               
                   
                   
                 EQVLPYVQKNKTQTISIFPWESAFKYRDALALRGAERIVEAG 
                   
               
               
                   
                   
                 MNNIFRVGGSHDGMRPLQRLVTYISHERPSHYTAKDVAVEIE 
                   
               
               
                   
                   
                 QTRFLEEDKFLVFVP 
                   
               
               
                   
               
               
                 4 
                 P1LuxD 
                 MENKSKYKTIDHVLCVEGNKKIHVWETLPEENSPKRKNTIIIA 
                 158 
               
               
                   
                   
                 SGFARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDE 
                   
               
               
                   
                   
                 FTMSIGKQSLLAVVDWLNTRKINNRGILASSLSARIVYASLSEI 
                   
               
               
                   
                   
                 NVSFLITAVGVVNLRYTLERALGFDYLSLPINELPNNLDFEGH 
                   
               
               
                   
                   
                 KLGAEVFARDCLDFGWEDLTSTINSMMYLDIPFIAFTANNDN 
                   
               
               
                   
                   
                 WVKQDEVITLLSNIRSNRCKIYSLLGSSHDLGENLVVLRNFYQ 
                   
               
               
                   
                   
                 SVTKAAIAMDNDRLDIDVDIIEPSFEHLTIATVNERRMKIEIEN 
                   
               
               
                   
                   
                 QAISLS 
                   
               
               
                   
               
               
                 5 
                 P1LuxE 
                 MTSYVDKQEIIASSEIDDLIFSSDPLAWSYDEQEKIRNKFVLDA 
                 159 
               
               
                   
                   
                 FRNHYKHCQEYRHYCQVHKVDDNITEIDDIPVFPTSVFKFTRL 
                   
               
               
                   
                   
                 LTSQENEIESWFTSSGTSGLKSQVARNRLSIERLLGSVSYGMK 
                   
               
               
                   
                   
                 YVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSLVELLYPTTF 
                   
               
               
                   
                   
                 TVMEERIDFVKTLNSLERIKNQGKDICLIGSPYFIYLLCQYMK 
                   
               
               
                   
                   
                 DKNISFYGDKNLYIITGGGWKSYEKESLKRDDFNHLLFDTFN 
                   
               
               
                   
                   
                 LNNISQIRDIFNQVELNTCFFEDEMQRKRVPPWVYARALDPET 
                   
               
               
                   
                   
                 LKPVPDGMPGLMSYMDASSTSYPAFIVTDDVGIMSREYGQYP 
                   
               
               
                   
                   
                 GVLVEILRRVNTRAQKGCALSLNQAFNS 
                   
               
               
                   
               
               
                 6 
                 SePetF 
                 MATYKVTLVNAAEGLNTTIDVADDTYILDAAEEQGIDLPYSC 
                 160 
               
               
                   
                   
                 RAGACSTCAGKVVSGTVDQSDQSFLDDDQIAAGFVLTCVAY 
                   
               
               
                   
                   
                 PTSDVTIETHKEEDLY 
                   
               
               
                   
               
               
                 7 
                 SePetH 
                 MLNASVAGGAATTTYGNRLFIYEVIGLRQAEGEPSDSSIRRSG 
                 161 
               
               
                   
                   
                 STFFKVPYSRMNQEMQRILRLGGKIVSIRPAEEAAANNGAAP 
                   
               
               
                   
                   
                 LQAAAEEPAAAPTPAPAAKKHSAEDVPVNIYRPNKPFVGKVL 
                   
               
               
                   
                   
                 SNEPLVQEGGIGVVQHLTFDISEGDLRYIEGQSIGIIPDGTDDK 
                   
               
               
                   
                   
                 GKPHKLRLYSIASTRHGDHVDDKTVSLCVRQLQYQNEAGETI 
                   
               
               
                   
                   
                 NGVCSTFLCGLKPGDDVKITGPVGKEMLLPADTDANVIMMG 
                   
               
               
                   
                   
                 TGTGIAPFRAYLWRMFKDNERAINSEYQFNGKAWLIFGIPTT 
                   
               
               
                   
                   
                 ANILYKEELEALQAQYPDNFRLTYAISREQKNEAGGRMYIQD 
                   
               
               
                   
                   
                 RVAEHADEIWNLLKDEKTHVYICGLRGMEDGIDQAMTVAAA 
                   
               
               
                   
                   
                 KEDVVWSDYQRTLKKAGRWHVETY 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Codon optimized gene sequences. 
               
             
          
           
               
                 NT 
                 Gene 
                   
                 SEQ 
               
               
                 ID 
                 name 
                 Sequence 
                 ID NO 
               
               
                   
               
               
                  1 
                 Orf1594 
                 GGATCCAAAACAATGTTCGGTTTAATAGGTCACTTAACAAGTTT 
                 162 
               
               
                   
                   
                 AGAACAAGCCAGAGATGTCAGTAGAAGAATGGGTTACGATGA 
                   
               
               
                   
                   
                 ATACGCAGACCAAGGTTTAGAATTTTGGTCTTCAGCCCCACCTC 
                   
               
               
                   
                   
                 AAATCGTAGATGAAATTACAGTTACCTCTGCTACTGGTAAAGT 
                   
               
               
                   
                   
                 CATTCATGGTAGATACATCGAATCATGTTTCTTGCCAGAAATGT 
                   
               
               
                   
                   
                 TGGCTGCAAGAAGATTCAAAACTGCAACAAGAAAGGTTTTGAA 
                   
               
               
                   
                   
                 TGCAATGTCCCATGCCCAAAAGCACGGTATCGATATTTCCGCAT 
                   
               
               
                   
                   
                 TGGGTGGTTTTACAAGTATAATCTTCGAAAACTTCGATTTGGCT 
                   
               
               
                   
                   
                 AGTTTGAGACAAGTTAGAGACACTACATTGGAATTCGAAAGAT 
                   
               
               
                   
                   
                 TCACCACTGGTAACACCCACACTGCTTACGTCATTTGTAGACAA 
                   
               
               
                   
                   
                 GTAGAAGCCGCTGCAAAAACCTTGGGTATAGATATCACACAAG 
                   
               
               
                   
                   
                 CCACCGTTGCTGTTGTCGGTGCTACTGGTGACATCGGTTCCGCA 
                   
               
               
                   
                   
                 GTATGCAGATGGTTGGATTTGAAATTGGGTGTTGGTGACTTAAT 
                   
               
               
                   
                   
                 CTTGACAGCTAGAAACCAAGAAAGATTGGATAACTTGCAAGCA 
                   
               
               
                   
                   
                 GAATTAGGTAGAGGTAAAATCTTGCCATTGGAAGCCGCTTTGC 
                   
               
               
                   
                   
                 CTGAAGCCGATTTTATCGTTTGGGTCGCTTCTATGCCACAAGGT 
                   
               
               
                   
                   
                 GTAGTTATTGATCCAGCTACCTTAAAACAACCTTGCGTTTTGAT 
                   
               
               
                   
                   
                 AGACGGTGGTTATCCTAAAAATTTGGGTTCTAAGGTTCAAGGT 
                   
               
               
                   
                   
                 GAAGGTATCTATGTCTTGAACGGTGGTGTCGTAGAACATTGTTT 
                   
               
               
                   
                   
                 CGATATAGACTGGCAAATCATGTCAGCAGCCGAAATGGCAAGA 
                   
               
               
                   
                   
                 CCTGAAAGACAAATGTTTGCCTGCTTCGCTGAAGCAATGTTGTT 
                   
               
               
                   
                   
                 AGAATTTGAAGGTTGGCACACTAATTTCTCTTGGGGTAGAAAC 
                   
               
               
                   
                   
                 CAAATTACAATAGAAAAGATGGAAGCCATCGGTGAAGCCTCTG 
                   
               
               
                   
                   
                 TTAGACACGGTTTCCAACCTTTAGCCTTAGCAATCTGAAAGCTT 
                   
               
               
                   
               
               
                  2 
                 Orf1593 
                 ATCTAGTTTTATTACAGCGGCCGCAAAACAATGCCACAATTAG 
                 163 
               
               
                   
                   
                 AAGCCTCCTTAGAATTAGACTTTCAATCAGAATCATATAAAGA 
                   
               
               
                   
                   
                 TGCTTACAGTAGAATCAACGCAATCGTCATTGAAGGTGAACAA 
                   
               
               
                   
                   
                 GAAGCATTTGATAACTACAACAGATTGGCAGAAATGTTACCAG 
                   
               
               
                   
                   
                 ATCAAAGAGACGAATTGCATAAATTGGCCAAGATGGAACAAA 
                   
               
               
                   
                   
                 GACACATGAAAGGTTTCATGGCTTGTGGTAAAAATTTGTCCGTT 
                   
               
               
                   
                   
                 ACTCCTGATATGGGTTTCGCACAAAAGTTTTTCGAAAGATTGCA 
                   
               
               
                   
                   
                 TGAAAACTTCAAAGCTGCAGCCGCTGAGGGTAAAGTTGTCACA 
                   
               
               
                   
                   
                 TGTTTGTTGATCCAATCTTTGATAATCGAATGCTTTGCTATCGC 
                   
               
               
                   
                   
                 AGCCTATAATATCTACATTCCAGTCGCTGATGCATTCGCCAGAA 
                   
               
               
                   
                   
                 AGATTACCGAAGGTGTAGTTAGAGACGAATATTTGCACAGAAA 
                   
               
               
                   
                   
                 CTTCGGTGAAGAATGGTTGAAGGCAAACTTCGATGCTTCTAAG 
                   
               
               
                   
                   
                 GCAGAATTGGAAGAAGCTAATAGACAAAACTTGCCTTTAGTCT 
                   
               
               
                   
                   
                 GGTTGATGTTAAATGAAGTAGCCGATGACGCTAGAGAATTGGG 
                   
               
               
                   
                   
                 TATGGAAAGAGAATCATTAGTTGAAGACTTCATGATCGCATAC 
                   
               
               
                   
                   
                 GGTGAAGCCTTAGAAAACATCGGTTTTACTACCAGAGAAATAA 
                   
               
               
                   
                   
                 TGAGAATGTCCGCATACGGTTTGGCAGCAGTCTAAGAGCTC 
                   
               
               
                   
               
               
                  3 
                 NpFAD 
                 TCTAGTTTTATTACAGCGGCCGCAAAACAATGCAACAATTAAC 
                 164 
               
               
                   
                   
                 AGACCAATCAAAGGAATTAGACTTCAAATCAGAAACTTACAAA 
                   
               
               
                   
                   
                 GATGCCTACTCCAGAATCAACGCAATCGTCATTGAAGGTGAAC 
                   
               
               
                   
                   
                 AAGAAGCACATGAAAACTACATCACCTTGGCCCAATTATTACC 
                   
               
               
                   
                   
                 AGAATCCCATGATGAATTGATCAGATTGTCTAAGATGGAATCA 
                   
               
               
                   
                   
                 AGACACAAAAAGGGTTTTGAAGCCTGTGGTAGAAATTTGGCTG 
                   
               
               
                   
                   
                 TTACTCCTGACTTACAATTTGCCAAAGAATTTTTCTCTGGTTTGC 
                   
               
               
                   
                   
                 ACCAAAACTTCCAAACTGCTGCAGCCGAGGGTAAAGTTGTCAC 
                   
               
               
                   
                   
                 ATGTTTGTTGATCCAATCATTAATAATCGAATGCTTTGCTATCG 
                   
               
               
                   
                   
                 CTGCATATAATATCTACATTCCAGTTGCCGATGACTTCGCTAGA 
                   
               
               
                   
                   
                 AAAATTACAGAAGGTGTAGTTAAGGAAGAATATTCCCATTTGA 
                   
               
               
                   
                   
                 ACTTTGGTGAAGTCTGGTTAAAAGAACACTTCGCAGAGAGTAA 
                   
               
               
                   
                   
                 GGCCGAATTGGAATTAGCAAATAGACAAAACTTGCCTATCGTC 
                   
               
               
                   
                   
                 TGGAAAATGTTAAATCAAGTAGAAGGTGACGCTCATACCATGG 
                   
               
               
                   
                   
                 CAATGGAAAAGGATGCTTTGGTTGAAGACTTCATGATTCAATA 
                   
               
               
                   
                   
                 CGGTGAAGCATTATCAAACATAGGTTTTTCTACCAGAGACATT 
                   
               
               
                   
                   
                 ATGAGATTGAGTGCTTACGGTTTGATAGGTGCTTGAGAGCTC 
                   
               
               
                   
               
               
                  4 
                 Orf880/ 
                 CTAAGTTTTATTACAGCGGCCGCAAAACAATGGCTACATTGAA 
                 165 
               
               
                   
                 OleT 
                 GAGAGACAAGGGTTTAGACAACACATTGAAAGTATTGAAGCA 
                   
               
               
                   
                   
                 AGGTTACTTATACACCACCAACCAAAGAAATAGATTGAACACT 
                   
               
               
                   
                   
                 TCTGTTTTCCAAACAAAGGCATTAGGTGGTAAACCTTTCGTTGT 
                   
               
               
                   
                   
                 CGTAACTGGTAAAGAAGGTGCCGAAATGTTCTACAACAACGAT 
                   
               
               
                   
                   
                 GTTGTCCAAAGAGAAGGCATGTTGCCAAAGAGAATCGTTAACA 
                   
               
               
                   
                   
                 CTTTGTTCGGTAAAGGTGCCATCCATACAGTCGATGGTAAAAA 
                   
               
               
                   
                   
                 GCACGTAGACAGAAAAGCTTTGTTCATGTCATTGATGACTGAG 
                   
               
               
                   
                   
                 GGTAATTTGAACTACGTCAGAGAATTGACCAGAACTTTATGGC 
                   
               
               
                   
                   
                 ATGCCAATACACAAAGAATGGAATCTATGGATGAAGTCAACAT 
                   
               
               
                   
                   
                 ATACAGAGAATCAATCGTATTGTTGACAAAGGTTGGTACCAGA 
                   
               
               
                   
                   
                 TGGGCTGGTGTACAAGCACCACCTGAAGACATCGAAAGAATTG 
                   
               
               
                   
                   
                 CAACAGATATGGACATAATGATCGATTCCTTTAGAGCCTTGGG 
                   
               
               
                   
                   
                 TGGTGCTTTCAAAGGTTACAAAGCAAGTAAAGAAGCTAGAAGA 
                   
               
               
                   
                   
                 AGAGTTGAAGATTGGTTGGAAGAACAAATCATCGAAACCAGA 
                   
               
               
                   
                   
                 AAGGGTAACATTCATCCACCTGAAGGTACTGCCTTGTATGAATT 
                   
               
               
                   
                   
                 TGCTCACTGGGAAGATTACTTAGGTAACCCTATGGACTCCAGA 
                   
               
               
                   
                   
                 ACATGTGCTATTGATTTGATGAATACCTTCAGACCATTGATCGC 
                   
               
               
                   
                   
                 TATAAACAGATTCGTTTCTTTCGGTTTGCATGCAATGAATGAAA 
                   
               
               
                   
                   
                 ACCCTATAACCAGAGAAAAGATTAAATCAGAACCAGATTACGC 
                   
               
               
                   
                   
                 TTACAAGTTCGCACAAGAAGTTAGAAGATATTACCCATTTGTCC 
                   
               
               
                   
                   
                 CTTTCTTACCTGGTAAAGCTAAGGTTGATATCGACTTCCAAGGT 
                   
               
               
                   
                   
                 GTTACAATTCCAGCAGGTGTCGGTTTGGCCTTAGACGTATATGG 
                   
               
               
                   
                   
                 TACTACACATGATGAATCCTTGTGGGATGACCCTAATGAATTCA 
                   
               
               
                   
                   
                 GACCAGAAAGATTCGAAACATGGGATGGTAGTCCTTTTGACTT 
                   
               
               
                   
                   
                 AATTCCACAAGGTGGTGGTGACTACTGGACCAACCACAGATGC 
                   
               
               
                   
                   
                 GCTGGTGAATGGATTACCGTTATCATCATGGAAGAAACTATGA 
                   
               
               
                   
                   
                 AGTACTTCGCAGAAAAGATTACTTACGATGTACCTGAACAAGA 
                   
               
               
                   
                   
                 TTTGGAAGTTGACTTAAACTCTATTCCAGGTTATGTAAAGAGTG 
                   
               
               
                   
                   
                 GTTTCGTTATTAAAAATGTCAGAGAAGTAGTAGATAGAACTTG 
                   
               
               
                   
                   
                 AGAGCTC 
                   
               
               
                   
               
               
                  5 
                 npgA 
                 ATGGTGCAAGACACATCAAGCGCAAGCACTTCGCCAATTTTAA 
                 166 
               
               
                   
                   
                 CAAGATGGTACATCGACACCCGCCCTCTAACCGCCTCAACAGC 
                   
               
               
                   
                   
                 AGCCCTTCCTCTCCTTGAAACCCTCCAGCCCGCTGATCAAATCT 
                   
               
               
                   
                   
                 CCGTCCAAAAATACTACCATCTGAAGGATAAACACATGTCTCT 
                   
               
               
                   
                   
                 CGCCTCTAATCTGCTCAAATACCTCTTCGTCCACCGAAACTGTC 
                   
               
               
                   
                   
                 GCATCCCCTGGTCTTCAATCGTGATCTCTCGAACCCCAGATCCG 
                   
               
               
                   
                   
                 CACAGACGACCATGCTATATTCCACCCTCAGGCTCACAGGAAG 
                   
               
               
                   
                   
                 ACAGCTTCAAAGACGGATATACCGGCATCAACGTTGAGTTCAA 
                   
               
               
                   
                   
                 CGTCAGCCACCAAGCCTCAATGGTCGCGATCGCGGGAACAGCT 
                   
               
               
                   
                   
                 TTTACTCCCAATAGTGGTGGGGACAGCAAACTCAAACCCGAAG 
                   
               
               
                   
                   
                 TCGGAATTGATATTACGTGCGTAAACGAGCGGCAGGGACGGAA 
                   
               
               
                   
                   
                 CGGGGAAGAGCGGAGCCTGGAATCGCTACGTCAATATATTGAT 
                   
               
               
                   
                   
                 ATATTCTCGGAAGTGTTTTCCACTGCAGAGATGGCCAATATAA 
                   
               
               
                   
                   
                 GGAGGTTAGATGGAGTCTCATCATCCTCACTGTCTGCTGATCGT 
                   
               
               
                   
                   
                 CTTGTGGACTACGGGTACAGACTCTTCTACACTTACTGGGCGCT 
                   
               
               
                   
                   
                 CAAAGAGGCGTATATAAAAATGACTGGGGAGGCCCTCTTAGCA 
                   
               
               
                   
                   
                 CCGTGGTTACGGGAACTGGAATTCAGTAATGTCGTCGCCCCGG 
                   
               
               
                   
                   
                 CCGCTGTTGCGGAGAGTGGGGATTCGGCTGGGGATTTCGGGGA 
                   
               
               
                   
                   
                 GCCGTATACGGGTGTCAGGACGACTTTATATAAAAATCTCGTT 
                   
               
               
                   
                   
                 GAGGATGTGAGGATTGAAGTTGCTGCTCTGGGCGGTGATTACC 
                   
               
               
                   
                   
                 TATTTGCAACGGCTGCGAGGGGTGGTGGGATTGGAGCTAGTTC 
                   
               
               
                   
                   
                 TAGACCAGGAGGTGGTCCAGACGGAAGTGGCATCCGAAGCCA 
                   
               
               
                   
                   
                 GGATCCCTGGAGGCCTTTCAAGAAGTTAGATATAGAGCGAGAT 
                   
               
               
                   
                   
                 ATCCAGCCCTGTGCGACTGGGGTGTGTAATTGCCTATCCTAA 
                   
               
               
                   
               
               
                  6 
                 SynAAC 
                 ATGGACTCAGGTCACGGTGCTCAATCAAGAATCAAGTTAGGTC 
                 167 
               
               
                   
                   
                 AAACAGGTTACAAGTTATCAACATATTTCTGCAAAAGTGGTCC 
                   
               
               
                   
                   
                 TAATTGGGAAAACCAACCACAAATCCATTGGAACTCTTTATTTT 
                   
               
               
                   
                   
                 CAACTGTCAAGATCCAATTGTCCTTATTCCCTTCTTCATTTCACT 
                   
               
               
                   
                   
                 TAATCATGGTAACTCCAATTAATTACCATAGTATCCACTGTTTG 
                   
               
               
                   
                   
                 GCAGATATTTGGGCCATAACAGGTGAAAATTTCGCTGATATTG 
                   
               
               
                   
                   
                 TAGCATTGAACGACAGACATTCTCACCCACCTGTTACCTTGACT 
                   
               
               
                   
                   
                 TACGCACAATTAAGAGAAGAAATTACAGCCTTTGCTGCTGGTT 
                   
               
               
                   
                   
                 TGCAATCATTAGGTGTTACCCCTCATCAACACTTAGCTATTTTC 
                   
               
               
                   
                   
                 GCAGATAATTCCCCAAGATGGTTTATAGCAGACCAAGGTAGTA 
                   
               
               
                   
                   
                 TGTTGGCAGGTGCCGTTAACGCTGTTAGATCAGCTCAAGCAGA 
                   
               
               
                   
                   
                 AAGACAAGAATTGTTGTACATCTTGGAAGATTCCAATAGTAGA 
                   
               
               
                   
                   
                 ACATTGATCGCAGAAAACAGACAAACCTTGTCTAAATTGGCTT 
                   
               
               
                   
                   
                 TAGATGGTGAAACCATTGACTTGAAGTTAATAATCTTGTTGACT 
                   
               
               
                   
                   
                 GATGAAGAAGTTGCCGAAGACTCAGCTATACCACAATATAATT 
                   
               
               
                   
                   
                 TCGCACAAGTCATGGCCTTAGGTGCTGGTAAAATTCCAACTCCT 
                   
               
               
                   
                   
                 GTACCAAGACAAGAAGAAGATTTGGCTACCTTAATATACACTT 
                   
               
               
                   
                   
                 CTGGTACTACAGGTCAACCAAAGGGTGTTATGTTGTCACATGG 
                   
               
               
                   
                   
                 TAATTTGTTGCACCAAGTTAGAGAATTGGATTCCGTCATCATTC 
                   
               
               
                   
                   
                 CTAGACCAGGTGACCAAGTTTTGAGTATTTTACCATGTTGGCAT 
                   
               
               
                   
                   
                 TCCTTGGAAAGAAGTGCTGAATATTTCTTGTTATCCAGAGGTTG 
                   
               
               
                   
                   
                 CACAATGAACTACACCAGTATCAGACATTTCAAGGGTGACGTT 
                   
               
               
                   
                   
                 AAGGACATAAAGCCTCATCACATAGTAGGTGTTCCAAGATTGT 
                   
               
               
                   
                   
                 GGGAATCTTTATATGAAGGTGTCCAAAAGACTTTTAGAGAAAA 
                   
               
               
                   
                   
                 GTCACCTGGTCAACAAAAATTGATTAATTTCTTTTTCGGTATCT 
                   
               
               
                   
                   
                 CACAAAAGTACATATTGGCAAAGAGAATCGCCAACAACTTGTC 
                   
               
               
                   
                   
                 TTTAAACCATTTGCACGCCTCAGCTATTGCAAGATTGGTAGCTA 
                   
               
               
                   
                   
                 GATGTCAAGCATTGGTTTTATCTCCATTGCATTATTTGGGTGAC 
                   
               
               
                   
                   
                 AAAATCGTATACCACAAGGTTAGACAAGCCGCTGGTGGTAGAT 
                   
               
               
                   
                   
                 TGGAAACTTTAATTTCTGGTGGTGGTGCCTTGGCTAGACATTTG 
                   
               
               
                   
                   
                 GATGACTTCTATGAAATCACCTCAATTCCTGTCTTAGTAGGTTA 
                   
               
               
                   
                   
                 CGGTTTAACAGAAACCGCCCCAGTCACAAATGCTAGAGTACAT 
                   
               
               
                   
                   
                 AAGCACAACTTAAGATATTCCAGTGGTAGACCTATCCCTTTTAC 
                   
               
               
                   
                   
                 TGAAATCAGAATCGTTGATATGGAAACTAAGGAAGACTTGCCA 
                   
               
               
                   
                   
                 CCTGAAACACAAGGTTTGGTCTTAATTAGAGGTCCTCAAGTAA 
                   
               
               
                   
                   
                 TGCAAGGTTATTACAATAAGCCAGAAGCAACTGCCAAGGTATT 
                   
               
               
                   
                   
                 AGATCAAGAAGGTTGGTTCGATTCCGGTGACTTGGGTTGGGTT 
                   
               
               
                   
                   
                 ACACCACAAAACGATTTGATATTAACTGGTAGAGCTAAAGACA 
                   
               
               
                   
                   
                 CAATCGTTTTATCTAATGGTGAAAACGTCGAACCTCAACCAATT 
                   
               
               
                   
                   
                 GAAGATGCATGCTTAAGATCCGCCTACATAGATCAAATCATGT 
                   
               
               
                   
                   
                 TGGTTGGTCAAGACCAAAAGAGTTTGGGTGCTTTAATCGTCCC 
                   
               
               
                   
                   
                 AAACTTCGATGCTTTACAAAAATGGGCAGAAACCAAGAACTTG 
                   
               
               
                   
                   
                 CAAATCACTGTTCCTGAACCATCTGCCTCTTCAGAGGGTATGCA 
                   
               
               
                   
                   
                 AGCATCTGGTTTGTATGATCCTCAAGTTGTCGGTTTGATGAGAT 
                   
               
               
                   
                   
                 CAGAATTACATAGAGAAGTTAGAGATAGACCAGGTTACAGAGC 
                   
               
               
                   
                   
                 AGATGACCAAATCAAAGATTTCAGATTCATTCCTGCTCCATTTT 
                   
               
               
                   
                   
                 CTTTAGAAAACGGTATGATGACTCAAACATTGAAATTGAAGAG 
                   
               
               
                   
                   
                 ACCTGTAGTCACCCAAACTTACCAACACTTGATAGACGAAATG 
                   
               
               
                   
                   
                 TTCTGA 
                   
               
               
                   
               
               
                  7 
                 MmCAR 
                 ATGTCACCTATCACCAGAGAAGAAAGATTAGAAAGAAGAATA 
                 168 
               
               
                   
                   
                 CAAGACTTATACGCCAACGATCCTCAATTCGCCGCTGCCAAGC 
                   
               
               
                   
                   
                 CAGCAACAGCCATCACCGCTGCAATTGAAAGACCAGGTTTGCC 
                   
               
               
                   
                   
                 ATTGCCTCAAATCATCGAAACTGTTATGACAGGTTATGCTGATA 
                   
               
               
                   
                   
                 GACCTGCTTTGGCACAAAGATCAGTAGAATTTGTTACAGATGC 
                   
               
               
                   
                   
                 AGGTACTGGTCATACTACATTGAGATTGTTACCACACTTCGAAA 
                   
               
               
                   
                   
                 CTATCTCTTACGGTGAATTATGGGACAGAATTTCTGCCTTGGCT 
                   
               
               
                   
                   
                 GATGTTTTATCAACCGAACAAACTGTTAAACCTGGTGACAGAG 
                   
               
               
                   
                   
                 TCTGTTTGTTGGGTTTTAATTCTGTTGACTACGCAACTATAGAT 
                   
               
               
                   
                   
                 ATGACATTGGCCAGATTAGGTGCAGTAGCCGTTCCATTGCAAA 
                   
               
               
                   
                   
                 CCTCTGCCGCTATTACTCAATTACAACCAATAGTCGCTGAAACA 
                   
               
               
                   
                   
                 CAACCTACCATGATAGCAGCCTCTGTAGATGCTTTGGCAGACG 
                   
               
               
                   
                   
                 CCACTGAATTGGCTTTATCAGGTCAAACTGCAACAAGAGTCTT 
                   
               
               
                   
                   
                 AGTATTCGACCATCACAGACAAGTTGATGCCCATAGAGCTGCT 
                   
               
               
                   
                   
                 GTTGAATCCGCTAGAGAAAGATTGGCAGGTAGTGCCGTTGTCG 
                   
               
               
                   
                   
                 AAACTTTAGCTGAAGCAATAGCTAGAGGTGACGTTCCAAGAGG 
                   
               
               
                   
                   
                 TGCTTCTGCTGGTTCTGCTCCTGGTACAGACGTCTCCGATGACA 
                   
               
               
                   
                   
                 GTTTGGCATTGTTAATCTATACCTCTGGTTCAACTGGTGCCCCA 
                   
               
               
                   
                   
                 AAAGGTGCTATGTACCCTAGAAGAAATGTTGCTACATTTTGGA 
                   
               
               
                   
                   
                 GAAAGAGAACCTGGTTCGAAGGTGGTTACGAACCATCTATCAC 
                   
               
               
                   
                   
                 TTTGAACTTCATGCCTATGTCACATGTTATGGGTAGACAAATCT 
                   
               
               
                   
                   
                 TGTATGGTACTTTATGCAACGGTGGTACAGCATACTTTGTTGCC 
                   
               
               
                   
                   
                 AAGTCTGACTTGTCAACATTATTCGAAGATTTGGCTTTAGTCAG 
                   
               
               
                   
                   
                 ACCAACTGAATTAACATTCGTCCCTAGAGTATGGGATATGGTTT 
                   
               
               
                   
                   
                 TTGACGAATTTCAATCAGAAGTCGATAGAAGATTGGTAGATGG 
                   
               
               
                   
                   
                 TGCTGACAGAGTAGCTTTAGAAGCACAAGTTAAGGCAGAAATA 
                   
               
               
                   
                   
                 AGAAACGATGTTTTGGGTGGTAGATATACATCTGCCTTAACCG 
                   
               
               
                   
                   
                 GTTCTGCTCCAATATCAGACGAAATGAAGGCTTGGGTAGAAGA 
                   
               
               
                   
                   
                 ATTGTTAGATATGCATTTGGTTGAAGGTTACGGTTCAACTGAAG 
                   
               
               
                   
                   
                 CTGGTATGATATTAATCGACGGTGCAATTAGAAGACCAGCCGT 
                   
               
               
                   
                   
                 TTTGGATTATAAATTGGTTGATGTCCCTGACTTGGGTTACTTTTT 
                   
               
               
                   
                   
                 AACTGATAGACCACACCCTAGAGGTGAATTGTTGGTTAAGACA 
                   
               
               
                   
                   
                 GATTCTTTGTTCCCAGGTTATTACCAAAGAGCTGAAGTTACAGC 
                   
               
               
                   
                   
                 AGATGTCTTTGATGCTGACGGTTTCTATAGAACCGGTGACATTA 
                   
               
               
                   
                   
                 TGGCAGAAGTCGGTCCTGAACAATTCGTATACTTAGATAGAAG 
                   
               
               
                   
                   
                 AAACAACGTTTTGAAATTGTCTCAGGGTGAATTTGTAACTGTTT 
                   
               
               
                   
                   
                 CAAAGTTGGAAGCTGTATTCGGTGACTCTCCATTAGTTAGACA 
                   
               
               
                   
                   
                 AATATATATATACGGTAATTCAGCCAGAGCTTATTTGTTAGCAG 
                   
               
               
                   
                   
                 TCATAGTACCAACACAAGAAGCCTTGGATGCTGTTCCTGTCGA 
                   
               
               
                   
                   
                 AGAATTGAAAGCCAGATTGGGTGACTCCTTGCAAGAAGTTGCA 
                   
               
               
                   
                   
                 AAGGCCGCTGGTTTGCAAAGTTACGAAATCCCAAGAGATTTCA 
                   
               
               
                   
                   
                 TCATCGAAACCACTCCTTGGACCTTAGAAAACGGTTTGTTAACT 
                   
               
               
                   
                   
                 GGTATCAGAAAATTGGCTAGACCACAATTGAAAAAGCATTACG 
                   
               
               
                   
                   
                 GTGAATTGTTAGAACAAATATATACTGACTTGGCCCACGGTCA 
                   
               
               
                   
                   
                 AGCTGATGAATTGAGATCCTTAAGACAAAGTGGTGCAGATGCC 
                   
               
               
                   
                   
                 CCAGTATTAGTTACAGTCTGTAGAGCAGCCGCTGCATTGTTAGG 
                   
               
               
                   
                   
                 TGGTTCCGCTAGTGATGTTCAACCTGACGCACATTTTACCGATT 
                   
               
               
                   
                   
                 TGGGTGGTGACTCTTTGTCAGCTTTATCTTTTACAAATTTGTTGC 
                   
               
               
                   
                   
                 ACGAAATCTTCGATATAGAAGTACCAGTTGGTGTCATTGTATCA 
                   
               
               
                   
                   
                 CCTGCTAACGATTTGCAAGCATTGGCAGATTATGTTGAAGCCG 
                   
               
               
                   
                   
                 CTAGAAAACCAGGTTCTTCAAGACCTACTTTTGCTTCTGTTCAT 
                   
               
               
                   
                   
                 GGTGCATCAAATGGTCAAGTTACAGAAGTCCACGCTGGTGACT 
                   
               
               
                   
                   
                 TGTCTTTGGATAAGTTCATTGATGCAGCCACTTTGGCCGAAGCT 
                   
               
               
                   
                   
                 CCAAGATTACCTGCTGCAAACACTCAAGTAAGAACAGTTTTGT 
                   
               
               
                   
                   
                 TAACCGGTGCTACTGGTTTCTTGGGTAGATATTTGGCATTAGAA 
                   
               
               
                   
                   
                 TGGTTAGAAAGAATGGATTTGGTTGACGGTAAATTGATTTGCTT 
                   
               
               
                   
                   
                 AGTCAGAGCAAAGTCCGACACTGAAGCAAGAGCCAGATTGGA 
                   
               
               
                   
                   
                 TAAAACATTCGATAGTGGTGACCCAGAATTGTTAGCACATTAC 
                   
               
               
                   
                   
                 AGAGCTTTAGCAGGTGACCACTTGGAAGTTTTAGCCGGTGACA 
                   
               
               
                   
                   
                 AGGGTGAAGCTGACTTGGGTTTAGATAGACAAACATGGCAAAG 
                   
               
               
                   
                   
                 ATTGGCTGATACCGTAGACTTAATCGTTGATCCAGCCGCTTTAG 
                   
               
               
                   
                   
                 TCAACCATGTATTGCCATACTCCCAATTGTTCGGTCCTAACGCA 
                   
               
               
                   
                   
                 TTGGGTACTGCTGAATTGTTGAGATTGGCTTTGACTTCTAAAAT 
                   
               
               
                   
                   
                 TAAGCCTTACTCCTACACCAGTACTATCGGTGTTGCAGATCAAA 
                   
               
               
                   
                   
                 TTCCACCTTCAGCCTTCACTGAAGATGCTGACATAAGAGTCATC 
                   
               
               
                   
                   
                 TCCGCAACAAGAGCCGTAGATGACAGTTATGCTAATGGTTACT 
                   
               
               
                   
                   
                 CCAACAGTAAATGGGCAGGTGAAGTTTTGTTAAGAGAAGCCCA 
                   
               
               
                   
                   
                 TGATTTGTGTGGTTTACCAGTTGCTGTCTTTAGATGCGACATGA 
                   
               
               
                   
                   
                 TTTTGGCAGATACAACCTGGGCCGGTCAATTGAACGTTCCAGA 
                   
               
               
                   
                   
                 TATGTTCACAAGAATGATCTTGTCCTTAGCAGCCACCGGTATAG 
                   
               
               
                   
                   
                 CTCCTGGTAGTTTCTATGAATTGGCTGCTGATGGTGCTAGACAA 
                   
               
               
                   
                   
                 AGAGCACATTACGATGGTTTGCCAGTTGAGTTTATTGCCGAAG 
                   
               
               
                   
                   
                 CTATCTCCACCTTAGGTGCTCAAAGTCAAGATGGTTTCCATACT 
                   
               
               
                   
                   
                 TATCACGTAATGAATCCATACGATGACGGTATTGGTTTGGACG 
                   
               
               
                   
                   
                 AATTTGTTGATTGGTTAAACGAATCTGGTTGTCCTATTCAAAGA 
                   
               
               
                   
                   
                 ATAGCTGATTATGGTGACTGGTTACAAAGATTCGAAACTGCTTT 
                   
               
               
                   
                   
                 GAGAGCATTACCAGATAGACAAAGACATTCCAGTTTGTTACCT 
                   
               
               
                   
                   
                 TTGTTACACAATTACAGACAACCAGAAAGACCTGTCAGAGGTT 
                   
               
               
                   
                   
                 CTATTGCTCCTACAGATAGATTCAGAGCCGCTGTACAAGAAGC 
                   
               
               
                   
                   
                 AAAAATAGGTCCAGATAAGGACATCCCTCATGTTGGTGCTCCT 
                   
               
               
                   
                   
                 ATTATCGTAAAGTATGTATCAGATTTGAGATTGTTGGGTTTGTT 
                   
               
               
                   
                   
                 GTAA 
                   
               
               
                   
               
               
                  8 
                 Fdx 
                 ATGCCAAAGATTGTTATTTTGCCTCATCAGGATCTCTGTCCTGA 
                 169 
               
               
                   
                   
                 TGGCGCTGTTCTGGAAGCTAATAGCGGTGAAACCATTCTCGAC 
                   
               
               
                   
                   
                 GCAGCGCTGCGTAACGGTATCGAGATTGAACACGCCTGTGAAA 
                   
               
               
                   
                   
                 AATCCTGTGCTTGCACCACCTGCCACTGCATCGTTCGTGAAGGT 
                   
               
               
                   
                   
                 TTTGACTCACTGCCGGAAAGCTCAGAGCAGGAAGACGACATGC 
                   
               
               
                   
                   
                 TGGACAAAGCCTGGGGACTGGAGCCGGAAAGCCGTTTAAGCTG 
                   
               
               
                   
                   
                 CCAGGCGCGCGTCACCGACGAAGATTTAGTGGTTGAAATCCCG 
                   
               
               
                   
                   
                 CGTTACACTATCAACCATGCGCGTGAGCATTAA 
                   
               
               
                   
               
               
                  9 
                 Fpr 
                 ATGGCTGATTGGGTAACAGGCAAAGTCACTAAAGTGCAGAACT 
                 170 
               
               
                   
                   
                 GGACCGACGCCCTGTTTAGTCTCACCGTTCACGCCCCCGTGCTT 
                   
               
               
                   
                   
                 CCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGA 
                   
               
               
                   
                   
                 CGGCGAACGCGTCCAGCGCGCCTACTCCTATGTAAACTCGCCC 
                   
               
               
                   
                   
                 GATAATCCCGATCTGGAGTTTTACCTGGTCACCGTCCCCGATGG 
                   
               
               
                   
                   
                 CAAATTAAGCCCACGACTGGCGGCACTGAAACCAGGCGATGAA 
                   
               
               
                   
                   
                 GTGCAGGTGGTTAGCGAAGCGGCAGGATTCTTTGTGCTCGATG 
                   
               
               
                   
                   
                 AAGTGCCGCACTGCGAAACGCTATGGATGCTGGCAACCGGTAC 
                   
               
               
                   
                   
                 AGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATT 
                   
               
               
                   
                   
                 TAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTAT 
                   
               
               
                   
                   
                 GCCGCCGACTTAAGCTATTTGCCACTGATGCAGGAACTGGAAA 
                   
               
               
                   
                   
                 AACGCTACGAAGGAAAACTGCGCATTCAGACGGTGGTCAGTCG 
                   
               
               
                   
                   
                 GGAAACGGCAGCGGGGTCGCTCACCGGACGGATACCGGCATTA 
                   
               
               
                   
                   
                 ATTGAAAGTGGGGAACTGGAAAGCACGATTGGCCTGCCGATGA 
                   
               
               
                   
                   
                 ATAAAGAAACCAGCCATGTGATGCTGTGCGGCAATCCACAGAT 
                   
               
               
                   
                   
                 GGTGCGCGATACACAACAGTTGCTGAAAGAGACCCGGCAGATG 
                   
               
               
                   
                   
                 ACGAAACATTTACGTCGCCGACCGGGCCATATGACAGCGGAGC 
                   
               
               
                   
                   
                 ATTACTGGTAA 
                   
               
               
                   
               
               
                 14 
                 CYP4G2 
                 ATGGACTCCGCCAACAACTCTACAGCCGGTCCTGCCACAGTAT 
                 171 
               
               
                   
                   
                 TGAATCCTATCTGGACAGCATTATTAGGTATTGCCGTCGTCGTC 
                   
               
               
                   
                   
                 TCATTGTACGAAATTTGGTTGAGAAACACTAGAAAGTACAAAT 
                   
               
               
                   
                   
                 TGACAGCAAATATGCCAAACCCACCTATGTTGCCTTTAATTGGT 
                   
               
               
                   
                   
                 AATGGTCATTTGGTTGCCCACTTAACAAACGCCGAAATTTTGGC 
                   
               
               
                   
                   
                 TAGAGGTATAGGTTATATGCAAACCTACGGTGGTGCCATGAGA 
                   
               
               
                   
                   
                 GGTTTCTTGGGTCCAATGTTAGTTGTCTTCTTGTGGAATGCTCCT 
                   
               
               
                   
                   
                 GATATCGAATTGATCTTAAGTACTCATACACACTTAGAAAAGT 
                   
               
               
                   
                   
                 CTATCGAATACAGATTTTTCAAACCTTGGTTTGGTGACGGTTTG 
                   
               
               
                   
                   
                 TTAATCAGTAACGGTCATCACTGGCAACATCACAGAAAGATGA 
                   
               
               
                   
                   
                 TAGCTCCAACTTTCCATCAATCCATCTTGAAAAGTTTTGTTCCT 
                   
               
               
                   
                   
                 GCTTTCGTCCAACACTCTAAAAAGGTAGTTGAAAGAATGGCAA 
                   
               
               
                   
                   
                 AGGAATTGGGTAAAGAATTTGATGTCCATGACTACATGTCACA 
                   
               
               
                   
                   
                 AACTACAGTAGAAATTTTGTTATCCACAGCTATGGGTGTTAAG 
                   
               
               
                   
                   
                 AAAGTTCCAGAAGATAATAAGTCATTAGAATACGCTAAAGCAG 
                   
               
               
                   
                   
                 TCGTAGATATGTGTGACATCATCCATAAGAGACAATTGAAGTT 
                   
               
               
                   
                   
                 TTTCTATAGAATGGATGCATTGTACAACTTATCTTCAATGTCCG 
                   
               
               
                   
                   
                 AAAAGGGTAAAAAGATGATGGATATCATCTTGGGTATGACAAG 
                   
               
               
                   
                   
                 AAAGGTTGTCACCGAAAGACAACAAAACTTCAACGCAGAAAG 
                   
               
               
                   
                   
                 TAGAGCCATCGTTGAAGAAGATGACGAAATTTCTAAGCAAAAG 
                   
               
               
                   
                   
                 CAACAAGCTAAAAAGAAAGAAGGTTTGAGAGATGACTTGGAT 
                   
               
               
                   
                   
                 GACATTGATGAAAATGACGTTGGTGCCAAGAAAAGATTGGCTT 
                   
               
               
                   
                   
                 TGTTAGACGCCATGATGGCTATGTCAAAGAATCCAGATGTTGA 
                   
               
               
                   
                   
                 ATGGACCGATAAAGACGTAATGGACGAAGTTAACACTATAATG 
                   
               
               
                   
                   
                 TTCGAAGGTCATGATACCACTTCCGCTGGTTCCAGTTTCGTTTT 
                   
               
               
                   
                   
                 GTGTATGTTGGGTATCTATAAGGATATCCAAGAAAAGGTCTTG 
                   
               
               
                   
                   
                 GCTGAACAAAAGGCAATCTTCGGTGACAATTTCTTGAGAGACT 
                   
               
               
                   
                   
                 GCACCTTCGCTGATACTATGGAAATGAAGTATTTGGAAAGAGT 
                   
               
               
                   
                   
                 TATCATGGAAACTTTGAGATTGTACCCACCTGTCCCATTAATTG 
                   
               
               
                   
                   
                 CAAGAAGAGCCGAATTTGATGTAAAGTTGGCATCTGGTCCATA 
                   
               
               
                   
                   
                 TACAATTCCTAAAGGTACAACCGTAGTTATAGCTCAATTTGCAG 
                   
               
               
                   
                   
                 TTCATAGAAATCCTCAATACTTCCCAAACCCTGAAAAATTTGAT 
                   
               
               
                   
                   
                 CCAGACAATTTCTTGCCTGAAAGAATGGCTAACAGACACTACT 
                   
               
               
                   
                   
                 ACTCTTTTATTCCATTCTCAGCAGGTCCTAGATCCTGCGTTGGT 
                   
               
               
                   
                   
                 AGAAAGTACGCCATGTTGAAGTTAAAGGTCTTGTTATCTACTAT 
                   
               
               
                   
                   
                 CATCAGAAATTACTCTGTACAATCAAACCAACAAGAAAAGGAC 
                   
               
               
                   
                   
                 TTCAAATTACAAGCAGATATTATATTGAAAATAGAAAATGGTT 
                   
               
               
                   
                   
                 TTAATATAATGTTGAATAGAAGACCTGAAGCAATGAAGGCAAT 
                   
               
               
                   
                   
                 GTAA 
                   
               
               
                   
               
               
                 15 
                 MdCPR 
                 ATGAGTGCCGAACACGTTGAAGAAGTAGTCAGTGAAGAACCAT 
                 172 
               
               
                   
                   
                 TTTTAGGTACATTGGATATTGCCTTATTAGTAGTATTATTAGTC 
                   
               
               
                   
                   
                 GGTGCCACTTGGTACTTCATGAGATCAAGAAAGAAAGAAGAAG 
                   
               
               
                   
                   
                 CTCCTATAAGATCATACTCAATCCAACCAACTACAGTCTCCACA 
                   
               
               
                   
                   
                 GTAAGTACCACTGAAAATTCCTTCATTAAAAAGTTGAAAGCAT 
                   
               
               
                   
                   
                 CTGGTAGATCATTAGTTGTCTTTTATGGTTCACAAACTGGTACA 
                   
               
               
                   
                   
                 GCTGAAGAATTTGCAGGTAGATTGGCCAAGGAAGGTTTAAGAT 
                   
               
               
                   
                   
                 ACAGAATGAAGGGTATGGTTGCTGACCCTGAAGAATGTGATAT 
                   
               
               
                   
                   
                 GGAAGAATTGTTACAAATGAAGGATATCCCAAATTCTTTGGCC 
                   
               
               
                   
                   
                 GTCTTTTGCTTAGCTACCTATGGTGAAGGTGACCCAACTGATAA 
                   
               
               
                   
                   
                 CGCTATGGAATTTTACGAATGGATTACAAACGGTGAAGTCGAT 
                   
               
               
                   
                   
                 TTGACCGGTTTAAATTATGCCGTATTTGGTTTGGGTAACAAAAC 
                   
               
               
                   
                   
                 TTATGAACATTACAATAAGGTTGCTATCTATGTCGATAAGAGAT 
                   
               
               
                   
                   
                 TGGAAGAATTAGGTGCAACAAGAGTTTTCGAATTGGGTTTAGG 
                   
               
               
                   
                   
                 TGACGACGATGCAAACATCGAAGACGATTTCATCACCTGGAAA 
                   
               
               
                   
                   
                 GACAGATTCTGGCCATCCGTTTGTGATTTCTTTGGTATTGAAGG 
                   
               
               
                   
                   
                 TAGTGGTGAAGAAGTCTTGATGAGACAATTCAGATTGTTAGAA 
                   
               
               
                   
                   
                 CAACCTGACGTACAACCAGATAGAATCTATACAGGTGAAATAG 
                   
               
               
                   
                   
                 CTAGATTGCATTCTATGCAAAACCAAAGACCACCTTTTGATGCT 
                   
               
               
                   
                   
                 AAGAATCCTTTCTTGGCATCAGTCATTGTAAACAGAGAATTAC 
                   
               
               
                   
                   
                 ACAAAGGTGGTGGTAGATCATGCATGCACATCGAATTGGACAT 
                   
               
               
                   
                   
                 TGATGGTTCAAAGATGAGATATGACGCAGGTGACCATATCGCC 
                   
               
               
                   
                   
                 ATGTACCCAATTAATGATAAAATCTTAGTTGAAAAATTGGGTA 
                   
               
               
                   
                   
                 AATTGTGTGACGCTAATTTGGATACTGTCTTTTCTTTAATCAAC 
                   
               
               
                   
                   
                 ACCGACACTGATTCTTCTAAGAAACACCCATTCCCTTGCCCAAC 
                   
               
               
                   
                   
                 AACCTATAGAACCGCATTGACTCATTACTTAGAAATCACAGCC 
                   
               
               
                   
                   
                 ATTCCTAGAACCCACATATTGAAGGAATTAGCAGAATATTGTT 
                   
               
               
                   
                   
                 CCGACGAAAAGGATAAGGAATTTTTGAGAAACATGGCCAGTAT 
                   
               
               
                   
                   
                 TACACCAGAGGGTAAAGAAAAGTACCAAAACTGGATACAAAA 
                   
               
               
                   
                   
                 CTCCAGTAGAAACATCGTTCATATCTTGGAAGATATAAAATCTT 
                   
               
               
                   
                   
                 GTAGACCACCTATAGATCATATTTGTGAATTGTTGCCTAGATTA 
                   
               
               
                   
                   
                 CAACCAAGATACTACTCTATCTCTTCATCCAGTAAGTTGTATCC 
                   
               
               
                   
                   
                 TACTAACGTTCATATTACAGCTGTTTTAGTCCAATACGAAACAC 
                   
               
               
                   
                   
                 CAACCGGTAGAGTAAATAAGGGTGTTGCAACTTCTTACATGAA 
                   
               
               
                   
                   
                 GGAAAAGAACCCTTCAGTTGGTGAAGTAAAGGTTCCAGTCTTT 
                   
               
               
                   
                   
                 ATAAGAAAGTCCCAATTCAGATTGCCTACTAAGAGTGAAATCC 
                   
               
               
                   
                   
                 CAATTATAATGGTTGGTCCTGGTACAGGTTTAGCACCTTTTAGA 
                   
               
               
                   
                   
                 GGTTTCATTCAAGAAAGACAATTCTTGAGAGACGGTGGTAAAG 
                   
               
               
                   
                   
                 TAGTTGGTGACACAATCTTGTACTTCGGTTGTAGAAAGAAAGA 
                   
               
               
                   
                   
                 CGAAGATTTCATCTATAGAGAAGAATTAGAACAATACGTTCAA 
                   
               
               
                   
                   
                 AACGGTACTTTGACATTGAAGACCGCCTTTTCAAGAGATCAAC 
                   
               
               
                   
                   
                 AAGAAAAGATATATGTAACTCATTTGATCGAACAAGACGCTGA 
                   
               
               
                   
                   
                 TTTGATTTGGAAAGTTATAGGTGAACAAAAGGGTCACTTCTAC 
                   
               
               
                   
                   
                 ATTTGCGGTGACGCTAAGAACATGGCAGTAGATGTTAGAAACA 
                   
               
               
                   
                   
                 TCTTGGTCAAAATTTTATCTACTAAGGGTAACATGAACGAATCA 
                   
               
               
                   
                   
                 GATGCTGTACAATACATTAAGAAAATGGAAGCCCAAAAGAGAT 
                   
               
               
                   
                   
                 ACTCCGCTGATGTTTGGAGTTAA 
                   
               
               
                   
               
               
                 16 
                 FacoAR 
                 ATGAATTATTTCTTGACAGGTGGTACAGGTTTTATCGGTAGATT 
                 173 
               
               
                   
                   
                 CTTGGTTGAAAAGTTGTTAGCCAGAGGTGGTACAGTTTATGTTT 
                   
               
               
                   
                   
                 TAGTTAGAGAACAATCTCAGGATAAGTTGGAAAGATTGAGAGA 
                   
               
               
                   
                   
                 AAGATGGGGTGCCGATGACAAACAAGTCAAGGCTGTAATAGGT 
                   
               
               
                   
                   
                 GACTTGACATCTAAAAATTTGGGTATCGATGCTAAGACCTTGA 
                   
               
               
                   
                   
                 AGTCTTTAAAGGGTAACATCGATCATGTATTCCACTTAGCTGCT 
                   
               
               
                   
                   
                 GTTTATGATATGGGTGCCGACGAAGAAGCTCAAGCCGCTACTA 
                   
               
               
                   
                   
                 ATATTGAAGGTACAAGAGCAGCCGTCCAAGCTGCTGAAGCTAT 
                   
               
               
                   
                   
                 GGGTGCTAAACATTTCCATCACGTTTCTTCAATCGCTGCTGCTG 
                   
               
               
                   
                   
                 GTTTGTTCAAGGGTATTTTTAGAGAAGACATGTTTGAAGAAGCT 
                   
               
               
                   
                   
                 GAAAAATTGGATCATCCATATTTGAGAACTAAGCACGAAAGTG 
                   
               
               
                   
                   
                 AAAAAGTTGTCAGAGAAGAATGTAAAGTTCCTTTTAGAATCTA 
                   
               
               
                   
                   
                 CAGACCTGGTATGGTTATTGGTCATTCTGAAACCGGTGAAATG 
                   
               
               
                   
                   
                 GATAAAGTTGACGGTCCATACTACTTTTTCAAGATGATCCAAA 
                   
               
               
                   
                   
                 AGATTAGACACGCTTTGCCACAATGGGTTCCTACTATCGGTATT 
                   
               
               
                   
                   
                 GAAGGTGGTAGATTAAACATCGTACCTGTTGATTTTGTAGTTGA 
                   
               
               
                   
                   
                 TGCATTGGACCATATTGCCCACTTAGAAGGTGAAGATGGTAAT 
                   
               
               
                   
                   
                 TGTTTCCATTTGGTCGATTCTGACCCATACAAAGTAGGTGAAAT 
                   
               
               
                   
                   
                 TTTAAACATATTTTGCGAAGCAGGTCACGCCCCTAGAATGGGT 
                   
               
               
                   
                   
                 ATGAGAATCGATTCAAGAATGTTCGGTTTCATTCCACCTTTTAT 
                   
               
               
                   
                   
                 AAGACAATCTATTAAAAATTTGCCACCTGTTAAGAGAATTACT 
                   
               
               
                   
                   
                 GGTGCTTTGTTAGATGACATGGGTATTCCACCTTCTGTTATGTC 
                   
               
               
                   
                   
                 ATTCATAAACTACCCAACCAGATTTGACACTAGAGAATTGGAA 
                   
               
               
                   
                   
                 AGAGTTTTGAAGGGTACAGATATAGAAGTCCCAAGATTACCTT 
                   
               
               
                   
                   
                 CTTATGCTCCAGTTATATGGGATTACTGGGAAAGAAACTTAGA 
                   
               
               
                   
                   
                 TCCAGATTTGTTTAAAGATAGAACATTGAAGGGTACTGTAGAG 
                   
               
               
                   
                   
                 GGTAAAGTTTGTGTCGTAACAGGTGCTACCTCCGGTATTGGTTT 
                   
               
               
                   
                   
                 GGCTACAGCAGAAAAATTGGCCGAAGCTGGTGCAATCTTGGTT 
                   
               
               
                   
                   
                 ATTGGTGCAAGAACTAAGGAAACATTGGATGAAGTTGCCGCTA 
                   
               
               
                   
                   
                 GTTTAGAAGCAAAAGGTGGTAATGTCCATGCCTATCAATGTGA 
                   
               
               
                   
                   
                 TTTCTCTGACATGGATGACTGCGATAGATTCGTTAAGACTGTCT 
                   
               
               
                   
                   
                 TGGATAATCATGGTCACGTTGATGTATTAGTTAATAACGCTGGT 
                   
               
               
                   
                   
                 AGATCCATAAGAAGAAGTTTGGCATTATCTTTTGATAGATTCCA 
                   
               
               
                   
                   
                 TGACTTCGAAAGAACAATGCAATTGAACTACTTCGGTTCAGTT 
                   
               
               
                   
                   
                 AGATTGATTATGGGTTTTGCCCCAGCTATGTTGGAAAGAAGAA 
                   
               
               
                   
                   
                 GAGGTCATGTTGTCAATATATCCAGTATCGGTGTATTAACAAAC 
                   
               
               
                   
                   
                 GCTCCTAGATTCTCAGCATACGTTTCTTCAAAATCAGCTTTGGA 
                   
               
               
                   
                   
                 CGCATTTTCCAGATGCGCAGCCGCTGAATGGTCCGATAGAAAC 
                   
               
               
                   
                   
                 GTCACCTTTACTACAATTAACATGCCATTGGTAAAGACCCCAAT 
                   
               
               
                   
                   
                 GATTGCTCCTACTAAAATCTATGATTCTGTTCCAACCTTGACTC 
                   
               
               
                   
                   
                 CTGACGAAGCAGCCCAAATGGTTGCAGATGCCATAGTCTACAG 
                   
               
               
                   
                   
                 ACCAAAGAGAATCGCTACTAGATTGGGTGTCTTCGCACAAGTA 
                   
               
               
                   
                   
                 TTGCATGCTTTGGCACCTAAGATGGGTGAAATCATCATGAACA 
                   
               
               
                   
                   
                 CAGGTTACAGAATGTTTCCAGATTCACCAGCTGCTGCTGGTTCT 
                   
               
               
                   
                   
                 AAGAGTGGTGAAAAACCTAAGGTTTCCACAGAACAAGTAGCAT 
                   
               
               
                   
                   
                 TTGCCGCCATTATGAGAGGTATCTATTGGTAA 
                   
               
               
                   
               
               
                 17 
                 RtACC1 
                 ATGCCATTCTCTGGCGAGGCGAAGGCGGTCAACGGATCGCACT 
                 174 
               
               
                   
                   
                 CGGTCGACGAGGCGCCGAAGAACCCCAAGTACGACCATGGGC 
                   
               
               
                   
                   
                 GGGTCGTAAAGTACCTCGGCGGCAACTCGCTCGAATCTGCGCC 
                   
               
               
                   
                   
                 CCCTTCCAAGGTCGCCGACTGGGTCAGGGAGCGTGGTGGACAC 
                   
               
               
                   
                   
                 ACCGTCATCACAAAGATCCTCATCGCCAACAATGGTATCGCCG 
                   
               
               
                   
                   
                 CAGTCAAGGAGATCCGCTCGGTGCGCAAGTGGGCGTACGAGAC 
                   
               
               
                   
                   
                 GTTCGGAAGCGAGCGCGCGATCGAGTTTACCGTCATGGCGACC 
                   
               
               
                   
                   
                 CCGGAGGACCTCAAGGTCAACGCAGACTACATCCGCATGGCCG 
                   
               
               
                   
                   
                 ATCAGTACGTCGAGGTTCCCGGTGGAACCAACAACAACAACTA 
                   
               
               
                   
                   
                 CGCCAACGTCGATGTCATCGTCGATGTTGCCGAGCGCGCAGGC 
                   
               
               
                   
                   
                 GTCCACGCCGTCTGGGCAGGATGGGGCCACGCCTCCGAGAACC 
                   
               
               
                   
                   
                 CCCGCCTTCCCGAGTCGCTCGCCGCCTCGAAGCACAAGATCGT 
                   
               
               
                   
                   
                 CTTCATCGGTCCTCCCGGCTCCGCCATGCGCTCGCTCGGAGACA 
                   
               
               
                   
                   
                 AGATCTCGTCGACCATCGTCGCGCAGCACGCCCAGGTTCCGTG 
                   
               
               
                   
                   
                 CATGGACTGGTCCGGCCAGGGCGTCGACCAAGTCACCCAGTCG 
                   
               
               
                   
                   
                 CCCGAGGGCTACGTTACTGTCGCCGACGACGTCTACCAGCAGG 
                   
               
               
                   
                   
                 CCTGTGTGCACGACGCCGACGAGGGTCTCGCCCGCGCGTCGAG 
                   
               
               
                   
                   
                 GATCGGATACCCCGTCATGATCAAGGCGTCCGAGGGAGGAGGA 
                   
               
               
                   
                   
                 GGAAAGGGTATTCGCAAGGTCGAGAAGGAGCAGGACTTTAAG 
                   
               
               
                   
                   
                 CAGGCCTTCCAGGCTGTCCTCACCGAGGTTCCCGGCTCGCCCGT 
                   
               
               
                   
                   
                 CTTTATCATGAAGCTCGCCGGCGCAGCTCGCCACCTCGAGGTCC 
                   
               
               
                   
                   
                 AGGTTCTCGCCGACCAGTACGGCAACGCCATCTCGCTCTTCGGC 
                   
               
               
                   
                   
                 CGTGACTGCTCGGTTCAGCGTCGCCACCAGAAGATCATCGAAG 
                   
               
               
                   
                   
                 AGGCGCCCGTCACCATCGCCAAGCCCGACACGTTCGAGCAGAT 
                   
               
               
                   
                   
                 GGAAAAGTCGGCCGTCCGCCTTGCCAAGCTCGTCGGCTACGTC 
                   
               
               
                   
                   
                 TCGGCGGGTACCGTCGAGTTCCTCTACTCGGCTGCCGACGACA 
                   
               
               
                   
                   
                 AGTTTGCCTTCCTCGAGCTCAACCCGCGTCTCCAGGTCGAGCAC 
                   
               
               
                   
                   
                 CCGACCACCGAGATGGTTTCGGGCGTCAACCTTCCCGCCGCCC 
                   
               
               
                   
                   
                 AGCTCCAGGTCGCTATGGGTGTTCCCCTCCATCGCATCCGCGAC 
                   
               
               
                   
                   
                 ATCCGCACGCTCTACGGCAAGGCACCCAACGGCAGCAGCGAGA 
                   
               
               
                   
                   
                 TCGATTTCGACTTCGAGAACCCCGAGTCGGCCAAGACGCAGCG 
                   
               
               
                   
                   
                 CAAGCCCTCGCCGAAGGGTCACGTCGTTGCCGTACGTATCACG 
                   
               
               
                   
                   
                 GCTGAGAACCCTGACGCCGGCTTCAAGCCGTCCATGGGTACTC 
                   
               
               
                   
                   
                 TCCAAGAGCTCAACTTCCGCTCGAGCACGAACGTCTGGGGTTA 
                   
               
               
                   
                   
                 CTTCTCCGTCGGCAGCGCCGGTGGACTGCACGAGTTTGCCGACT 
                   
               
               
                   
                   
                 CGCAGTTCGGCCACATCTTTGCGTACGGCTCGGACCGTTCCGAG 
                   
               
               
                   
                   
                 TCGCGCAAGAACATGGTCGTCGCGCTCAAGGAGCTCTCGATTC 
                   
               
               
                   
                   
                 GCGGTGACTTCCGCACGACCGTCGAGTACCTCATCAAGCTTCTC 
                   
               
               
                   
                   
                 GAGACGGACGCGTTCGAGCAGAACACGATCACGACCGCGTGG 
                   
               
               
                   
                   
                 CTCGACAGCCTCATCTCGGCTCGCCTGACCGCCGAGAGGCCCG 
                   
               
               
                   
                   
                 ACACGACTCTCGCCATCATCTGCGGCGCCGTTACCAAGGCCCA 
                   
               
               
                   
                   
                 CCTCGCTTCCGAGGCCAACATCGCCGAGTACAAGCGCATCCTC 
                   
               
               
                   
                   
                 GAGAAGGGTCAGAGCCCCGCCAAGGAGCTCCTCGCCACCGTCG 
                   
               
               
                   
                   
                 TCCCGCTCGAGTTCGTCCTCGAGGACGTCAAGTACCGCGCGAC 
                   
               
               
                   
                   
                 CGCCTCGCGCTCGTCGCCTTCGAGCTGGTCCATCTACGTCAACG 
                   
               
               
                   
                   
                 GCTCGAACGTCTCCGTCGGCATCCGCCCTCTCGCCGACGGCGGT 
                   
               
               
                   
                   
                 CTCCTCATCCTCCTTGACGGCCGCTCGTACACCTGCTACGCCAA 
                   
               
               
                   
                   
                 GGAGGAGGTCGGCGCGCTCCGCCTCTCGATCGACTCGAGGACC 
                   
               
               
                   
                   
                 GTCCTCATTGCTCAGGAGAACGACCCCACCCAGCTTCGCTCGCC 
                   
               
               
                   
                   
                 TTCACCCGGCAAGCTCGTCCGCTACTTCATCGAGTCCGGCGAGC 
                   
               
               
                   
                   
                 ACATCTCGAAGGGCGAGGCGTACGCTGAGATCGAGGTCATGAA 
                   
               
               
                   
                   
                 GATGATCATGCCCCTCATCGCTGCCGAGGACGGTATCGCGCAA 
                   
               
               
                   
                   
                 TTCATCAAGCAGCCGGGAGCGACGCTCGAGGCCGGCGACATCC 
                   
               
               
                   
                   
                 TCGGTATCTTGTCGCTCGACGACCCGAGCCGCGTCCACCACGCC 
                   
               
               
                   
                   
                 AAGCCGTTCGATGGCCAGCTTCCCGCCCTTGGCTTGCCCTCCAT 
                   
               
               
                   
                   
                 CGTCGGCAACAAGCCGCACCAGCGCTTCGCCTACCTCAAAGAC 
                   
               
               
                   
                   
                 GTGCTCTCAAACATCCTCATGGGCTACGACAACCAGGCCGTCA 
                   
               
               
                   
                   
                 TGCAGTCGAGCATCAAGGAGCTCATCTCGGTTCTTCGCAACCCC 
                   
               
               
                   
                   
                 GAGCTCCCCTACGGCGAGGCCAACGCTGTCCTCTCGACGCTTTC 
                   
               
               
                   
                   
                 GGGTCGCATCCCCGCCAAGCTCGAGCAGACCCTCCGCCAGTAC 
                   
               
               
                   
                   
                 ATCGACCAGGCTCACGAGTCTGGCGCCGAGTTCCCGTCCGCCA 
                   
               
               
                   
                   
                 AGTGCCGCAAGGCGATCGACACGACCCTTGAGCAGCTCCGCCC 
                   
               
               
                   
                   
                 CGCCGAGGCGCAGACTGTCCGCAACTTCCTCGTCGCGTTCGAC 
                   
               
               
                   
                   
                 GACATCGTCTACCGCTACCGCTCGGGCCTCAAGCACCACGAGT 
                   
               
               
                   
                   
                 GGTCAACGCTCGCCGGCATCTTTGCCGCGTACGCCGAGACGGA 
                   
               
               
                   
                   
                 GAAGCCGTTCAGCGGCAAGGACGGCGACGTCGTCCTCGAGCTC 
                   
               
               
                   
                   
                 CGCGACGCCCACCGCGACTCGCTCGACTCGGTCGTCAAGATCG 
                   
               
               
                   
                   
                 TTCTCTCGCACTACAAGGCTGCCTCGAAGAACTCGCTTGTCCTT 
                   
               
               
                   
                   
                 GCGCTCCTCGACATCGTCAAGGACTCGGACGCGGTTCCGCTCA 
                   
               
               
                   
                   
                 TCGAGCAGGTCGTCAGCCCTGCGCTCAAGGACCTCGCCGACCT 
                   
               
               
                   
                   
                 CGACTCGAAGGCCACGACTAAGGTCGCCCTGAAGGCCCGCGAG 
                   
               
               
                   
                   
                 GTGCTCATCCACATCCAGCTCCCCTCGCTCGACGAGCGCCTCGG 
                   
               
               
                   
                   
                 ACAGCTCGAGCAGATTCTCAAGGCCTCGGTGACGCCCACCGTT 
                   
               
               
                   
                   
                 TACGGCGAGCCCGGCCACGACCGCACTCCTCGCGGTGAAGTCC 
                   
               
               
                   
                   
                 TTAAGGACGTCATCGACTCGCGCTTCACCGTCTTTGACGTTCTC 
                   
               
               
                   
                   
                 CCGAGCTTCTTCCAGCACCAGGACCACTGGGTCTCGCTCGCCGC 
                   
               
               
                   
                   
                 GCTCGACACCTACGTCCGCCGCGCCTACCGCTCGTACAACCTCC 
                   
               
               
                   
                   
                 TCAACATCGAGCACATCGAGGCCGATGCCGCCGAGGACGAGCC 
                   
               
               
                   
                   
                 CGCGACGGTTGCCTGGTCGTTCCGCATGCGCAAGGCTGCGTCC 
                   
               
               
                   
                   
                 GAGTCTGAGCCGCCCACGCCCACGACCGGCCTCACGTCGCAGC 
                   
               
               
                   
                   
                 GCACCGCCTCGTACTCGGACTTGACGTTCCTCCTCAACAACGCC 
                   
               
               
                   
                   
                 CAGTCCGAGCCGATCCGCTACGGCGCGATGTTCTCGGTCCGCTC 
                   
               
               
                   
                   
                 GCTCGACCGCTTCCGCCAGGAGCTCGGTACCGTCCTCCGACACT 
                   
               
               
                   
                   
                 TCCCCGACTCGAACAAGGGCAAGCTCCAGCAGCAGCCTGCCGC 
                   
               
               
                   
                   
                 GTCGTCGAGCCAGGAGCAGTGGAACGTCATCAACGTCGCGCTC 
                   
               
               
                   
                   
                 ACGGTCCCCGCCAGCGCGCAGGTCGACGAGGACGCTCTCCGCG 
                   
               
               
                   
                   
                 CCGACTTTGCCGCTCACGTGAACGCGATGAGCGCCGAGATCGA 
                   
               
               
                   
                   
                 CGCTCGCGGCATGCGCCGCCTCACCCTCCTCATCTGCCGCGAGG 
                   
               
               
                   
                   
                 GCCAGTACCCGTCCTACTACACCGTCCGCAAGCAGGACGGCAC 
                   
               
               
                   
                   
                 CTGGAAGGAGCTCGAGACGATCCGCGACATCGAGCCCGCCCTC 
                   
               
               
                   
                   
                 GCCTTCCAGCTCGAGTTGGGCCGCCTCTCCAACTTCCACCTCGA 
                   
               
               
                   
                   
                 GCCGTGCCCCGTTGAGAACCGCCAGGTCCACGTCTACTACGCG 
                   
               
               
                   
                   
                 ACCGCCAAGGGCAACTCGTCCGACTGCCGCTTCTTCGTCCGCGC 
                   
               
               
                   
                   
                 ACTCGTCCGCCCTGGCCGTCTCCGCGGTAACATGAAGACGGCC 
                   
               
               
                   
                   
                 GACTACCTCGTCTCCGAGGCTGACCGCCTCGTCACCGATGTCCT 
                   
               
               
                   
                   
                 CGACTCGCTCGAGGTCGCCAGCTCGCAGCGCCGCGCTGCCGAC 
                   
               
               
                   
                   
                 GGCAACCACATCTCGCTCAACTTCCTGTACTCTCTCCGTCTCGA 
                   
               
               
                   
                   
                 CTTTGACGAGGTCCAGGCTGCCCTCGCCGGCTTCATCGACCGCC 
                   
               
               
                   
                   
                 ACGGCAAGCGCTTCTGGCGTCTCCGCGTCACCGGCGCCGAGAT 
                   
               
               
                   
                   
                 CCGCATCGTCCTCGAGGACGCGCAGGGCAACATTCAGCCCATC 
                   
               
               
                   
                   
                 CGCGCCATCATCGAGAACGTCTCGGGTTTCGTCGTCAAGTACG 
                   
               
               
                   
                   
                 AGGCGTACCGCGAGGTCACGACCGACAAGGGCCAGGTCATCCT 
                   
               
               
                   
                   
                 CAAGTCGATCGGTCCGCAGGGCGCGTTGCACCTTCAGCCGGTC 
                   
               
               
                   
                   
                 AACTTCCCCTACCCGACCAAGGAGTGGCTTCAGCCGAAGCGCT 
                   
               
               
                   
                   
                 ACAAGGCCCACGTCGTCGGCACGACGTACGTCTACGACTTCCC 
                   
               
               
                   
                   
                 CGACCTTTTCCGCCAGGCAATCCGCAAGCAGTGGAAGGCGGCC 
                   
               
               
                   
                   
                 GGCAAGACTGCGCCCGCCGAGCTCCTCGTCGCCAAGGAGCTCG 
                   
               
               
                   
                   
                 TCCTCGACGAGTTCGGCAAGCCTCAGGAGGTCGCCCGCCCGCC 
                   
               
               
                   
                   
                 TGGCACCAACAATATCGGCATGGTCGGCTGGATCTACACGATC 
                   
               
               
                   
                   
                 TTCACGCCCGAATACCCCTCTGGCCGCCGCGTCGTCGTCATCGC 
                   
               
               
                   
                   
                 GAACGACATCACGTTCAAGATTGGTTCGTTCGGCCCGGAGGAG 
                   
               
               
                   
                   
                 GACCGCTACTTCTTCGCCGTCACGCAGCTCGCGCGCCAACTTGG 
                   
               
               
                   
                   
                 CTTGCCGCGCGTCTACCTCTCGGCCAACTCGGGTGCTCGTCTCG 
                   
               
               
                   
                   
                 GCATTGCCGAGGAGCTCGTCGACTTGTTCAGCGTCGCGTGGGT 
                   
               
               
                   
                   
                 CGACAGCTCGCGGCCGGAGAAGGGCTTCAAGTACCTCTACCTA 
                   
               
               
                   
                   
                 ACCGCCGAGAAGCTCGGCGAGCTCAAGAACAAGGGCGAGAAG 
                   
               
               
                   
                   
                 AGCGTCATCACGAAGCGCATCGAGGACGAGGGCGAGACGCGC 
                   
               
               
                   
                   
                 TACCAGATCACCGACATCATCGGCTTGCAGGAGGGTCTCGGTG 
                   
               
               
                   
                   
                 TCGAGTCGCTCAAGGGCTCTGGCCTCATCGCCGGTGAGACGTC 
                   
               
               
                   
                   
                 GCGCGCGTACGACGACATCTTCACGATCACGCTCGTCACCGCC 
                   
               
               
                   
                   
                 CGCTCGGTCGGTATCGGTGCGTACCTCGTCCGCCTCGGCCAGCG 
                   
               
               
                   
                   
                 TGCCGTCCAGGTCGAGGGCCAGCCGATCATCCTCACCGGTGCC 
                   
               
               
                   
                   
                 GGCGCGCTCAACAAGGTCCTCGGTCGCGAGGTGTACTCGTCCA 
                   
               
               
                   
                   
                 ACTTGCAGCTCGGCGGCACGCAGATCATGTACAAGAACGGTGT 
                   
               
               
                   
                   
                 CTCGCACTTGACGGCCGCCAACGACCTCGAGGGTGTCCTCAGC 
                   
               
               
                   
                   
                 ATCGTCCAGTGGCTCGCCTTCGTCCCCGAGCACCGCGGCGCGC 
                   
               
               
                   
                   
                 CTCTCCCGATCATGCCTTCGCCCGTCGACCCGTGGGACCGCTCG 
                   
               
               
                   
                   
                 ATCGACTACACGCCCATCAAGGGCGCGTACGACCCGCGCTGGT 
                   
               
               
                   
                   
                 TCCTCGCCGGCAAGACGGACGAGGCCGACGGTCGCTGGCTCTC 
                   
               
               
                   
                   
                 TGGCTTCTTCGACAAGGGCTCGTTCCAGGAGACGCTCTCGGGCT 
                   
               
               
                   
                   
                 GGGCGCAGACCGTCGTCGTCGGTCGCGCTCGCCTCGGCGGCAT 
                   
               
               
                   
                   
                 CCCCATGGGCGCCATCGCGGTCGAGACCCGCACCATCGAGCGC 
                   
               
               
                   
                   
                 GTCGTGCCCGCCGACCCTGCCAACCCTCTCTCGAACGAGCAGA 
                   
               
               
                   
                   
                 AGATCATGGAGGCCGGTCAGGTCTGGTATCCCAACAGCTCGTT 
                   
               
               
                   
                   
                 CAAGACGGGACAGGCGATCTTCGACTTCAACCGCGAGGGTCTC 
                   
               
               
                   
                   
                 CCGCTCATCATCTTCGCCAACTGGCGCGGCTTCTCGGGCGGCCA 
                   
               
               
                   
                   
                 GCAGGACATGTTCGACGAGGTCCTCAAGCGCGGTTCGCTCATT 
                   
               
               
                   
                   
                 GTCGACGGTCTCTCGGCGTACAAGCAGCCCGTCTTCGTCTACAT 
                   
               
               
                   
                   
                 CGTCCCGAACGGCGAACTTCGCGGCGGTGCTTGGGTCGTCCTC 
                   
               
               
                   
                   
                 GACCCGTCGATCAACGCCGAGGGCATGATGGAGATGTACGTCG 
                   
               
               
                   
                   
                 ACGAGACTGCTCGCGCCGGTGTCCTCGAGCCCGAGGGCATCGT 
                   
               
               
                   
                   
                 CGAGATCAAGCTCCGCAAGGACAAGCTCCTCGCCCTCATGGAC 
                   
               
               
                   
                   
                 CGCCTCGACCCGACCTACCACGCCCTCCGCGTCAAGTCGACCG 
                   
               
               
                   
                   
                 ACGCTTCGCTCTCGCCCGCCGACGCCGCGCAGGCCAAGACCGA 
                   
               
               
                   
                   
                 GCTCGCCGCGCGCGAGAAGCAGCTCATGCCGATCTACCAGCAG 
                   
               
               
                   
                   
                 GTCGCGCTCCAGTTCGCCGACTCGCACGACAAGGCCGGCCGCA 
                   
               
               
                   
                   
                 TCCTCAGCAAGGGCTGCGCGCGCGAGGCCCTCGAGTGGTCGAA 
                   
               
               
                   
                   
                 CGCTCGTCGCTACTTCTACGCCCGCCTCCGCCGCCGTCTCGCCG 
                   
               
               
                   
                   
                 AGGAGGCCGCCGTCAAGCGTCTCGGCGACGCCGACCCGACCCT 
                   
               
               
                   
                   
                 CTCGCGCGACGAGCGCCTCGCCATCGTCCACGACGCCGTCGGC 
                   
               
               
                   
                   
                 CAGGGTGTCGACCTCAACAACGACCTCGCTGCTGCCGCCGCGT 
                   
               
               
                   
                   
                 TCGAGCAGGGCGCCGCCGCCATCACCGAGCGCGTCAAGCTCGC 
                   
               
               
                   
                   
                 GCGCGCGACGACCGTCGCCTCGACTCTCGCGCAGCTCGCGCAG 
                   
               
               
                   
                   
                 GACGACAAGGAGGCTTTCGCCGCCTCGCTCCAGCAGGTCCTCG 
                   
               
               
                   
                   
                 GCGACAAGCTCACCGCCGCCGACCTCGCCCGCATCCTCGCCTA 
                   
               
               
                   
                   
                 G 
                   
               
               
                   
               
               
                 20 
                 RtFAS1 
                 ATGAACGGCCGAGCGACGCGGAGCGTGACTGGGACGTCGACG 
                 175 
               
               
                   
                   
                 CCGGTCCACACGGCGACGACCCGACCCCTCGTCCTCTTGCACCC 
                   
               
               
                   
                   
                 CTCGACCCAAACCCGCATCTCGCTGCACGTCCCCTCCACGTCGC 
                   
               
               
                   
                   
                 AGGAATGGATCGCCGCCGAAGTCGCGCGCGACACCTTCCAGGA 
                   
               
               
                   
                   
                 CTGGCTTCACGCTGCCGAGAAGAGCGGAAACCTCGTCGGATTC 
                   
               
               
                   
                   
                 GAGGCGGCCGAGCTTGACGACGAGCAGGCTGGCGAGGGCGAC 
                   
               
               
                   
                   
                 GACGAGAAGGAGCTCGTCCTCACCGCCTACTTCTTGAAGCACG 
                   
               
               
                   
                   
                 TTGCCGGCCTTCTCCCCTTCCCGTCGACAGCTACCTCCCCCGCC 
                   
               
               
                   
                   
                 ACCGCCGCCGTCCTCCTCGCCGCCTTCAACCACTTTGCGTCCGT 
                   
               
               
                   
                   
                 CTACCTCAGCGGAACCGATGTTCACACCCTCACTGCCTCGCTCG 
                   
               
               
                   
                   
                 CTGCTCCCGTCCGCGCTCTCGTCATCTCGTCCTTCTTCCTCGCCA 
                   
               
               
                   
                   
                 AGACCAAGCTCGAGGTCGAGGGACTCGGCAAGGTCTTGCCCAA 
                   
               
               
                   
                   
                 GCAGTCCGAGTCGGCGCTCCTGCAGAAGGCTGCGACCGGCCAG 
                   
               
               
                   
                   
                 GCAGAGGTCTTCGCTCTCTTCGGTGGTCAGGGAATGAACGAGG 
                   
               
               
                   
                   
                 TCTACTTTGACGAGCTCCAGACCCTCCACGACCTTTACACCCCG 
                   
               
               
                   
                   
                 CTGCTTACGCCCTTCCTCGCCCGCGCCTCCGAACACCTCGTCTC 
                   
               
               
                   
                   
                 TCTCGCTGCCGCCGAGCAGCACACCCTCCTTTACGACCACTCGC 
                   
               
               
                   
                   
                 TCGACGCCCTTGCCTGGCTGCAAGATCCCTCTACCCGCCCCGAA 
                   
               
               
                   
                   
                 GTCCCCTACCTCGCGACTTGCGCCGTCTCGCTCCCTCTCATCGG 
                   
               
               
                   
                   
                 TCTCACTCAGCTCTGCCAGTACGTCGTGTACGGCAAGGGCTCGT 
                   
               
               
                   
                   
                 CGCTCGGTCCCGCCGAGCTCGGCGCCAAGTTCAAGGGCGCGAC 
                   
               
               
                   
                   
                 CGGCCACTCGCAGGGTGTCGTCTCGGCTCTTGTCATCGCGCACG 
                   
               
               
                   
                   
                 AGTACCCTCCCGCGTCCAAGGACGGCAGCGACGCGTGGGAGCC 
                   
               
               
                   
                   
                 TTTCTACGAGCAGGCCCTTCGCGGTTTGACCGTCCTCTTCCAGA 
                   
               
               
                   
                   
                 TCGGTCTCCAGGGCACGCTCGCCTTCCCCTCCATCGCCATTTCG 
                   
               
               
                   
                   
                 CCCGCTCTCGAGTCGAGCTCGGTCGAGAATGGCGAGGGTGTCC 
                   
               
               
                   
                   
                 CGACTGCCATGCTTGCCGTCACCGGCCTCGACCTCAAGTCGCTC 
                   
               
               
                   
                   
                 GAGAAGAAGATCGCCGAGGTCAATGGGCACGTCAAGTCTGAG 
                   
               
               
                   
                   
                 GGCCGCGACGAGACCGTCTCGATCAGTCTCTACAACGGTGCGA 
                   
               
               
                   
                   
                 GGGCGTTCGTCGTCACTGGTGCGCCGAAGGACCTCGTCGGTCT 
                   
               
               
                   
                   
                 CGCCGACGGCCTTCGCAAGAACCGCGCGCCGGCCGGCAAGGAC 
                   
               
               
                   
                   
                 CAGTCGAAGATCCCGCACTCGAAGCGTCTCCCCGTCTTCTCGAT 
                   
               
               
                   
                   
                 GCGCTTCCTCCCCATCAACGTTCCCTACCACTCGCATCTCCTCC 
                   
               
               
                   
                   
                 AAGGCGCGACCGAGAAGGCGCTCGCGACGTTCTCGGCTGAGGA 
                   
               
               
                   
                   
                 GGCCGCCCACTGGGCGCCTTCATCGTTCACCTGCGCCGTCTACA 
                   
               
               
                   
                   
                 ACACCGAGGACGGCTCCGACATGCGCCAGCTCTCGGCTTCGTC 
                   
               
               
                   
                   
                 GGTTCTCGAGTCGGTCTTCCAGCAGATCTTCACCTCGCCCATTC 
                   
               
               
                   
                   
                 ACTGGGTCTCGCACGCCACCAACTTCCCCTCGTCCGCGACGCAC 
                   
               
               
                   
                   
                 GCCATCGATTTCGGCACGGGCGGCGCGAGCGGCATCGGTTCGC 
                   
               
               
                   
                   
                 TCTGCGCGCGCAACTGGGAGGGCCGCGGTATCCGCACGATTAT 
                   
               
               
                   
                   
                 GCTCGGCAACCGCGGCGAGGGCGTTGGTGCCGGCAAGGAGGCT 
                   
               
               
                   
                   
                 TGGGGCAAGAAGGTCCCGACCGAGGAGAAGTGGAACGAGCGC 
                   
               
               
                   
                   
                 TTCCACCCTCGCCTCGTCCGCACCAGCGACGGCAAGATCCACCT 
                   
               
               
                   
                   
                 CGACACGCCCTTCTCGCGCCTCCTCTCGAAGCCGCCCCTCATGG 
                   
               
               
                   
                   
                 TCGGTGGTATGACCCCGACGACCGTCAAGGCCGGCTTCGTCTC 
                   
               
               
                   
                   
                 GGCCGTTCTCCGCGCGGGCTACCACATCGAGCTCGCTGGCGGC 
                   
               
               
                   
                   
                 GGTCACTACAACGAGAAGGCTGTCCGTGCCAAGGTCGCCGAGA 
                   
               
               
                   
                   
                 TCCAGAAGCTCGTGAACAAGCCCGGCATGGGCATCACCCTCAA 
                   
               
               
                   
                   
                 CTCGCTCTACATCAACCAGCGCCAGTGGACGTTCCAGTTCCCGC 
                   
               
               
                   
                   
                 TCTGGGCCAAGATGAAGCAGGAGGGCGAGCCCGTCGAGGGTCT 
                   
               
               
                   
                   
                 CTGTGTTGCTGCCGGTATTCCCTCAACCGAGAAGGCCAAGGAG 
                   
               
               
                   
                   
                 ATCATCGACACGCTCCGCGAGGCCGGCATCAAGCACGTCTCGT 
                   
               
               
                   
                   
                 TCAAGCCCGGTTCGGTCGACGGCATCCGCCAGGTCGTCAACAT 
                   
               
               
                   
                   
                 CGCCTCCGCCAACCCCGACTTCCCCATCATCCTCCAGTGGACTG 
                   
               
               
                   
                   
                 GTGGTCGCGCCGGCGGTCACCACTCGTGCGAGGACTTCCACGC 
                   
               
               
                   
                   
                 CCCGATCCTCGCGACGTACGCTTCGATCCGTCAGCACCCCAAC 
                   
               
               
                   
                   
                 ATCAAGCTCGTCGCCGGCTCTGGCTTCGGCTCGGCTGAGGGAT 
                   
               
               
                   
                   
                 GCTACCCTTACCTTTCGGGCGAGTGGTCGGAGAAGCAGTACGG 
                   
               
               
                   
                   
                 CGTCGCGCGCATGCCGTTCGACGGCTTCATGTTTGCTTCGTGGG 
                   
               
               
                   
                   
                 TCATGGTCGCCAAGGAGGCGCACACGAGCGAGTCGGTCAAGCA 
                   
               
               
                   
                   
                 GCTCATCGTCGACGCGCCTGGTGTCGAGGATGGCCAGTGGGAG 
                   
               
               
                   
                   
                 CAGACGTACGACAAGCCGACCGGCGGCATCCTCACCGTCAACT 
                   
               
               
                   
                   
                 CGGAGCTTGGCGAGCCGATCCACAAGGTCGCGACTCGTGGTGT 
                   
               
               
                   
                   
                 CAAGCTGTGGGCCGAGTTCGACAAGAAGGTCTTCTCGCTGTCG 
                   
               
               
                   
                   
                 AAGGAGAAGCAGCTCGCATGGCTCGCCGACAACAAGAAGTAC 
                   
               
               
                   
                   
                 GTTATCGACCGCCTCAACGCCGATTTCCAGAAGCCCTGGTTCCC 
                   
               
               
                   
                   
                 CGCCAAGGCCGACGGCTCTCCTTGCGACCTTGCCGACATGACC 
                   
               
               
                   
                   
                 TACGCCGAGGTCAACGCCCGCCTCGTCCGCCTCATGTACGTCGC 
                   
               
               
                   
                   
                 GCACGAGAAGCGCTGGATCGACCCGTCGCTCCGCAACCTCGTC 
                   
               
               
                   
                   
                 GGCGACTGGATCCGCCGTGTTGAGGAGCGTCTCTCGAACGTCA 
                   
               
               
                   
                   
                 ACGACTCGGGCATCAAGATCTCGGCACTCCAGTCGTACTCGGA 
                   
               
               
                   
                   
                 GCTGAACGAGCCTGAGGCGTTCCTCAAGCAGTTCCTCGCCCAG 
                   
               
               
                   
                   
                 TACCCGCAGGCCGAGGACCAGATCCTCGCCTCCGCCGACGTTT 
                   
               
               
                   
                   
                 CCTACTTCCTCGCCATCTCTCAACGCCCCGGACAGAAGCCCGTC 
                   
               
               
                   
                   
                 CCCTTCATCCCCGTCCTCGACGCCAACTTCAGCATCTGGTTCAA 
                   
               
               
                   
                   
                 GAAGGACTCGCTGTGGCAGGCCGAGGACATCGAGGCCGTCTTT 
                   
               
               
                   
                   
                 GACCAGGACCCGCAGCGTGTCTGCATCCTCCAGGGACCGGTCG 
                   
               
               
                   
                   
                 CCGCCAAGCACTGCACCTCGACGCAGACGCCCATCGCCGAGAT 
                   
               
               
                   
                   
                 GCTCGGCAACA7CGAGCACCAGCTCGTCAAGAACGTCCTGGAC 
                   
               
               
                   
                   
                 GACTACTACGGCGGCGACGAGTCCCAGATCCCGACTATCGACT 
                   
               
               
                   
                   
                 ACCTCGCGCCCCCTCCCAAGCCGGTCGACGCCGGCGCTATCCTC 
                   
               
               
                   
                   
                 GCCGAGAACAACATCGCGCACTCGGTCGAGGAGCTCGCCGACG 
                   
               
               
                   
                   
                 GCGGCAAGAAGCATGTCTACTCGATCAACGGTGTCCTCCCGCC 
                   
               
               
                   
                   
                 GACGGGCGACTGGCATGCCGCACTCGCCGGCCCCAAGCTCGAC 
                   
               
               
                   
                   
                 TGGCTCCAGGCGTTCCTCTCCAACGTCTCGATTCAGGCGGGCGA 
                   
               
               
                   
                   
                 GCAGTCGATTCCTAACCCCGTCAAGAAGGTGCTGGCGCCGAGG 
                   
               
               
                   
                   
                 CACGGGCAGCGGGTCGAGCTCACCCTGAACAAGGACGGCCAG 
                   
               
               
                   
                   
                 CCCCTCAAGCTCGACGTCTTCGGCGGGCTCTGA 
                   
               
               
                   
               
               
                 21 
                 RtFAS2 
                 ATGGTCGCGGCGCAGGACTTGCCGCTCGCGCTGAGCATCAGCT 
                 176 
               
               
                   
                   
                 TCGCGCCCGAGTCGTCGACCATCTCGATGACGCTGTTCAACCA 
                   
               
               
                   
                   
                 GCCCGAGGCGTCGAAACCCGCCCTCCCCCTCGAGCTCAAGTAC 
                   
               
               
                   
                   
                 AAGTACGACCCCTCGACGCCGTACGCCCCGATCCACGAGATCA 
                   
               
               
                   
                   
                 CCGAGGACCGTAATCAGAGGATCAAGCAGCACTACTGGGACCT 
                   
               
               
                   
                   
                 CTGGGGCCTCGGCAACAAGGCAGACCAGGGCATCTCGCAGCTC 
                   
               
               
                   
                   
                 AAGATCACCGACGAGTTCCAGGGCGACCTCGTCACCATCTCGG 
                   
               
               
                   
                   
                 CCGACGAGATCGAGGCGTTCTGCCGTGTTGTCGGCATCGAGGG 
                   
               
               
                   
                   
                 CGAGGCGTACAAGCGCAACCACAAGGCCGGCATGCAGGTCCC 
                   
               
               
                   
                   
                 GCTCGACTTCGCCATCAAGCTCGGCTGGAAGGCCATCATGAAG 
                   
               
               
                   
                   
                 CCGATCTTCCCCTCGACGATTGACGGCGACCTGCTCAAGCTCGT 
                   
               
               
                   
                   
                 CCACCTCTCGAACGGCTTCCGCGTCCTCCCCGACACGCCCACAC 
                   
               
               
                   
                   
                 TCCAGGTTGGCGACGTCGTGACGACCACGTCGCGCATCGAATC 
                   
               
               
                   
                   
                 AATCACGAACTCGGACACGGGCAAAACCGTCTCGGTTCGCGGC 
                   
               
               
                   
                   
                 GTCATCTCGCTCGTCTCGTCCGCCGACTCGAAGGGCAAGGACG 
                   
               
               
                   
                   
                 CCTCGACCGAGGACCGCATCCCGCTCATCGAGGTCACCTCGTC 
                   
               
               
                   
                   
                 CTTCTTCTACCGCGGCAAGTTCAGCGACTACGCCCAGACATTCT 
                   
               
               
                   
                   
                 CCCGCGTCGCCCACCCGACCTACTCTGTCCCGATCACCACGCCC 
                   
               
               
                   
                   
                 GAGGCCGTCGCCGTCCTCCAGTCCAAGGAGTGGTTCCAGTGGG 
                   
               
               
                   
                   
                 ACGACGACTCGAAGCCCCTCGAGGTCGGCACCAAGCTCCAGTT 
                   
               
               
                   
                   
                 CAAGGTCGAGTCGAACTATGTCTACGCCGACAAGTCGTCCTAC 
                   
               
               
                   
                   
                 GCGATGGCTACCGTCACCGGCGGCGCGTACGTCATCACCCCCG 
                   
               
               
                   
                   
                 AGCTCAAGCTCGCTGTCAAGGTTGCCACGGTCGACTACACGTC 
                   
               
               
                   
                   
                 CGAGGGCGAGGGCGTCATCCAGGGCGACCCGGTCATCGAGTAC 
                   
               
               
                   
                   
                 CTCAAGCGCCACGGCTCGGCCCTCGACCAGCCCATCATGCTCG 
                   
               
               
                   
                   
                 AGAACGGCGGCTATTCGCTCACCAAGGCCGGCCAGTGCACCTT 
                   
               
               
                   
                   
                 CACGACGCCCGCGTCCAACCTCGACTACTCGCTCACCTCGGGC 
                   
               
               
                   
                   
                 GACACGAACCCGATTCACACGAACCCGTACTTTGCCTCGCTCG 
                   
               
               
                   
                   
                 CCTACCTCCCCGGCACCATCACGCACGGCATGCACTCGTCGGC 
                   
               
               
                   
                   
                 CCGCACGCGCAAGTTTGTCGAGCAGGTCGCCGCAGACAACGTC 
                   
               
               
                   
                   
                 GGCGCGCGCGTCCGCAAGTACGAGGTCGGCTTCACGGCCATGT 
                   
               
               
                   
                   
                 GCCTCCCCTCGCGCAAGATGGAGGTCCGCCTTAAGCACGTCGG 
                   
               
               
                   
                   
                 CATGACCGCGGACGGAAACCGCCTCATCAAGGTCGAGACCGTC 
                   
               
               
                   
                   
                 GACGTCGAGGGCGGCAACGTCGTTCTCAGCGGAACCGCCGAGG 
                   
               
               
                   
                   
                 TCGCCCAGGCTCCCACCGCGTACGTCTTCACCGGTCAAGGTTCG 
                   
               
               
                   
                   
                 CAAGAGCCCGGCATGGGCATGGAGCTCTACGCCAACTCGCCCG 
                   
               
               
                   
                   
                 TCGCCCGCGCCGTCTGGGACGAGGCTGACCGCCACCTCGGCGA 
                   
               
               
                   
                   
                 GGTCTACGGCTTCTCCATCCTCGAGATTGTCCGTACGAACCCCA 
                   
               
               
                   
                   
                 AGGAAAAGACTGTGCACTTCGGCGGGTTGAAAGGCCAAGCAA 
                   
               
               
                   
                   
                 CCCGTCAGAAGTACATGGACATGTCGTACACAACGACTGACCA 
                   
               
               
                   
                   
                 TGAGGGCAACGTTAAGACTCTCCCGCTCTTCGGCGACATCGAC 
                   
               
               
                   
                   
                 CTCCGTACCTCACGCTACACGTTCTCGTCGCCGACCGGTCTCCT 
                   
               
               
                   
                   
                 CTACGCCACCCAGTTCGCCCAGATCGCCCTCGTCGTAACGGAG 
                   
               
               
                   
                   
                 AAGGCCGCCTTCGAGGACATGCGCGCCAAGGGTCTCGTTCAGA 
                   
               
               
                   
                   
                 AGGACTGCGTCTTTGCCGGTCACTCGCTCGGAGAGTACTCGGCT 
                   
               
               
                   
                   
                 CTCGCCTCGATCGCCGACATCCTCCCCATCTCGGCCCTCGTCGA 
                   
               
               
                   
                   
                 CGTCGTCTTCTACCGCGGTATCACCATGCAGCGCGCCGTCGAAC 
                   
               
               
                   
                   
                 GCGACCACCTCAACCGCTCGTCGTACGGAATGGTCGCCGTCAA 
                   
               
               
                   
                   
                 CCCGAGCCGCATCGGCAAGAGCTTTGGCGACGCCGCCCTCCGC 
                   
               
               
                   
                   
                 GAGGTCGTCGACACCATCGCCCGCCGCGGAAACATCCTCATCG 
                   
               
               
                   
                   
                 AGGTCGTCAACTACAACGTCGAGGGACAGCAATACGTCGTCGC 
                   
               
               
                   
                   
                 CGGTCACCTCGTCGCCCTCCAATCCCTCACAAACGTCCTCAACT 
                   
               
               
                   
                   
                 TCCTCAAGATCCAGAAGATCGACCTCGCCAAGCTCACCGAGAC 
                   
               
               
                   
                   
                 GATGTCGATCGAGCAGGTCAAGGAGCACCTGTGCGAGATCGTC 
                   
               
               
                   
                   
                 GACGAGTGCGTCCAGAAGGCGCGCGACCTCCAGGCCAAGACG 
                   
               
               
                   
                   
                 GGCTTCATCACCCTCGAGCGCGGCTTTGCGACGATCCCGCTCCC 
                   
               
               
                   
                   
                 CGGTATCGACGTGCCGTTCCACTCGCGCTACCTCTGGGCGGGA 
                   
               
               
                   
                   
                 GTCATGCCGTTCCGCACTTACCTCTCGAAGAAGGTCAACCCGG 
                   
               
               
                   
                   
                 CGCACTTCAACGCCGACCTCCTCGTCGGCCGCTACATCCCCAAC 
                   
               
               
                   
                   
                 TTGACCGCCGTCCACTACGAGGTCTCGAAGGAGTACGCCGAAC 
                   
               
               
                   
                   
                 GCATCCACACCCAGACGTCGTCGCCGCGCCTCAACAAGATTCT 
                   
               
               
                   
                   
                 CAAGGCCTGGGACGAGGAGCGCTGGGGCGCACCCGAGAACCG 
                   
               
               
                   
                   
                 CAACAAGCTCGGCTACGCCATCCTCATCGAGCTCCTCGCGTACC 
                   
               
               
                   
                   
                 AGTTCGCCTCGCCCGTCCGCTGGATCGAGACGCAGGACATCCT 
                   
               
               
                   
                   
                 CTTCCGCGACTTCAAGTTTGAGCGCCTCGTCGAGCTTGGCCCGT 
                   
               
               
                   
                   
                 CGCCCACTCTCACCGGCATGGCTACGCGCACGCAGAAGCTCAA 
                   
               
               
                   
                   
                 GTACGACGCGCACGACTCGTCGGTCGGCATCAAGCGCTCGATC 
                   
               
               
                   
                   
                 TACTGCATCGCCAAGCACCAGAAGGAGATCTACTACCAGTTCG 
                   
               
               
                   
                   
                 ATGACGTTGCCGGCGAAGAGGCGCCCGCTCCTGCCGCAGTTGC 
                   
               
               
                   
                   
                 GCCTTCCGCTCCCGCTCCCAAGGCCGCCCCAGTCGCCGCCGCCC 
                   
               
               
                   
                   
                 CTCCCCCTCCCGCTCCTGTCGCTGCCGCGCCTGCCGCCGCCGTC 
                   
               
               
                   
                   
                 GCCGACGAGCCGCTCAAGGCTGTCGACACGCTCCGCATCATCA 
                   
               
               
                   
                   
                 TCGCGCAGAAGCTCAAGAAGCCCGTTGGCGAAGTCCCCCTCAC 
                   
               
               
                   
                   
                 CAAGTCGATCAAGGAGCTCGTCGGCGGCAAGTCGACCCTCCAG 
                   
               
               
                   
                   
                 AACGAGATTCTCGGCGACCTTCAAGGCGAGTTCAGCAGCGCGC 
                   
               
               
                   
                   
                 CTGAAAAGGGCGAGGAGATGCCTCTCCAGGAGCTCGGCGCGGC 
                   
               
               
                   
                   
                 CCTCCAGCAGGGCTACTCTGGCAAGCTCGGCAAGTACACCACC 
                   
               
               
                   
                   
                 GGCGTCATCTCGCGCATGATTGGCGCCAAGATGCCCGGCGGTT 
                   
               
               
                   
                   
                 TTGGTCTCTCCGCCGTCCAGGGTCACCTCGGCAAGACCTACGGC 
                   
               
               
                   
                   
                 CTCGGCGCCGGTCGCATCGATGGCGTCCTCCTCTTCGCCGTCAC 
                   
               
               
                   
                   
                 GCAGGAGCCGGCTAAGCGTCTCGCCAACGAGGGTGAGGCGAA 
                   
               
               
                   
                   
                 GGCTTGGGTCGACTCGGTCGCGCAAGGCTACGCCTCGATGGCT 
                   
               
               
                   
                   
                 GGCATCTCGCTCGCCGCCGGCGGTGGAGCTGCTGCTGCTGCCC 
                   
               
               
                   
                   
                 CCGCGATGGCGTTCGCCGCTCCGGCCGCAGCTGGCGGTGGAGC 
                   
               
               
                   
                   
                 GCCCGCTGCCGTCCCCGACGAGCCGCTCAAGGCGACCGACACG 
                   
               
               
                   
                   
                 CTTCGCGCCATCATCGCTCAGAAGCTCAAGAAGCAGATCCCCG 
                   
               
               
                   
                   
                 ACGTCCCCCTCACCAAGTCCATCAAGGACCTTGTCGGCGGCAA 
                   
               
               
                   
                   
                 GTCGACCCTGCAGAACGAGATCCTCGGCGACCTCCAGGGCGAG 
                   
               
               
                   
                   
                 TTCAGCAGTGCGCCCGAGAAGGGCGAGGAGATGCCGCTCCAGG 
                   
               
               
                   
                   
                 AGCTTGGCGCCGCACTCAACCAAGGCTACTCGGGCACGCTCGG 
                   
               
               
                   
                   
                 CAAGCACACGAGCGGTCTCGTCGCCCGCATGATGGGCGCCAAG 
                   
               
               
                   
                   
                 ATGCCCGGTGGCTTCGGTCTCTCGGCGGCGAAGGCGCACCTCT 
                   
               
               
                   
                   
                 CGAAGGCTCACGGTCTCGGGCCCGGCCGCACCGACGGCGCTCT 
                   
               
               
                   
                   
                 CCTCGTCGCGCTCACCAAGGAGCCCGAGAAACGTCTCGGTAGC 
                   
               
               
                   
                   
                 GAGGCCGACGCCAAGGCCTGGCTCGACGGCGTCGCTCAGGCGT 
                   
               
               
                   
                   
                 ACGCCTCGCAGGCTGGCATCACCCTCGGCGCTGGTGGAGGCGG 
                   
               
               
                   
                   
                 AGGCGGCGCGGCTGTCGGCGGCGCCGGCTTTATGATCAACACC 
                   
               
               
                   
                   
                 GAGCAGCTCGACAAGATGCAGGAGAAGCAGGACAACTTCGTCT 
                   
               
               
                   
                   
                 CGCAGCAGGTCGAGCTCTTCCTCCGCTACCTCGGCAAGGACTC 
                   
               
               
                   
                   
                 GCGCGAGGGCCACCGCCTCGCCGACATGCAGAAGGCAGAGGT 
                   
               
               
                   
                   
                 CGCCAACCTCCAGGAGAAGCTCGACTCGATCGCTCGCGAGCAC 
                   
               
               
                   
                   
                 GGCGACGCCTATGTCCAGGGCATCCAGCCCGTCTTCGACCCGC 
                   
               
               
                   
                   
                 TCAAGGCCCGCCACTTCAACTCGTCGTGGAACTGGGTCCGTCA 
                   
               
               
                   
                   
                 GGACGCGCTCATGATGTGGATGGACATCCTCTTCGGCCGCCTC 
                   
               
               
                   
                   
                 ACCACCGTCGACCGCGACATCACCGCTCGCTGCCTTGTCATCAT 
                   
               
               
                   
                   
                 GAACCGCGCCGACCCTTCTCTCATCGACTACATGCAGTACACC 
                   
               
               
                   
                   
                 ATCGACAACACCCCCGTCGAGCGCGGCGAGCATTACGTCCTCG 
                   
               
               
                   
                   
                 CCAAGCAATTCGGCCAGCAGCTCCTCGACAACTGCCGCGAGAT 
                   
               
               
                   
                   
                 GATCGGCCAGGCTCCGCTCTACAAGGACGTCACCTTCCCGACC 
                   
               
               
                   
                   
                 GCGCCCAAGACGACCGTCAACGCCAAGGGCGACATCATCACCG 
                   
               
               
                   
                   
                 AGGAGGTCAACCGCCCCGGCGTCTCTCGCCTCGAGAAGTATGT 
                   
               
               
                   
                   
                 CGCCGAGATGGCTGCCGGCTCAAAGGTCACCGTCGCCAGCGTC 
                   
               
               
                   
                   
                 AACCTCGACAAGGTCCAGGAGCAGGTCGAGAAGCTGTACAAG 
                   
               
               
                   
                   
                 CTCGTCAAGTCGCAGCCGCAGATTTCGAAGCAGCACATGACGT 
                   
               
               
                   
                   
                 CGATCAAGTCGCTGTACGCTGAGGTCGTTCGCGGTCTCGGCAA 
                   
               
               
                   
                   
                 GGACGCCGGCCCTCCTCCGGTCCACAAGGCCGGCACTCGCGCC 
                   
               
               
                   
                   
                 CGCCGCCCCTCGAGCCAGTTCCTCCGTCCCGCAGCCGTCTCCGA 
                   
               
               
                   
                   
                 GGCGACTTTCCTCCCCGAGGACAAGGTGCCTCTCCTGCACCTCA 
                   
               
               
                   
                   
                 AGCGCAAGATCGGCAACGACTGGCAATACTCGAGCAAGCTCAC 
                   
               
               
                   
                   
                 GTCGCTCTACCTCGACATCCTCAAGGAGATTGCCACGTCGGGT 
                   
               
               
                   
                   
                 GTCACCTTCGAGCACAAGAACGCGCTCATGACCGGTGTCGGCA 
                   
               
               
                   
                   
                 AGGGCTCCATCGGTATCGAGATCGTCAAGGGTCTCCTCGCTGG 
                   
               
               
                   
                   
                 TGGCGCTCGCGTCGTCATCACGACCTCGCGCTACTCGCGCTCGA 
                   
               
               
                   
                   
                 CTGTCGAGTACTACCAGGCGATCTACCAGGAGGTCGGCTCGAA 
                   
               
               
                   
                   
                 GGGCTCGTCGCTCACCGTCGTCCCCTTCAACCAGGGCTCGAAG 
                   
               
               
                   
                   
                 CAGGATGTCGAGGCGCTCGTCGACTTCATTTATTCGAAGGATA 
                   
               
               
                   
                   
                 AGGGTCTCGGCATGGACCTCGACTACATCCTCCCCTTCGCCGCC 
                   
               
               
                   
                   
                 CTTCCCGAGAACGGCCGCGAGATCGACGGCATCGACGACCGCT 
                   
               
               
                   
                   
                 CCGAGCTCGCCCACCGCATCATGCTCACCAACCTCCTCCGCCTC 
                   
               
               
                   
                   
                 CTCGGTGCCGTCAAGTCGAAGAAGGCCGCCCTCAAGCTCACGA 
                   
               
               
                   
                   
                 CCCGCCCAACCGAGGTCGTCCTCCCGCTTTCGCCGAACCACGG 
                   
               
               
                   
                   
                 CCTCTTCGGCAACGACGGTCTCTACTCGGAGTCGAAGATCTCGC 
                   
               
               
                   
                   
                 TCGAGACGCTCTTCAACCGCTGGAGCTCGGAGAGCTGGGGCGA 
                   
               
               
                   
                   
                 GTACCTCTGCCTCGCTGGCGCTGTCATCGGATGGACGCGCGGT 
                   
               
               
                   
                   
                 ACCGGTCTCATGTCGGCGACGAACTCGGTCGCCGAAGGTATCG 
                   
               
               
                   
                   
                 AGGCGCAGGGTTGCAGGACGTTCTCCGCCAAGGAGATGGCCTT 
                   
               
               
                   
                   
                 CAACATTCTCGGCCTCATGCACCCGCTCGTCTTCGACGTCGCGC 
                   
               
               
                   
                   
                 AGATCGAGCCTGTCTGGGCCGACCTCAACGGTGGCATGGACAA 
                   
               
               
                   
                   
                 GCTCCCCGACCTTGCCAACCTCACGACCGAGATCCGCAAGAAG 
                   
               
               
                   
                   
                 CTCAACCTCACCGCGTCGACCCGCCGCGCCATCGCCAAGGACA 
                   
               
               
                   
                   
                 ACTCGTTCGACTACAAGGTCGCGCACGGCCCGGCGATGGAGCA 
                   
               
               
                   
                   
                 GATACACCAGCGGATCAACGTCGCCCCGCGCGCCAACTTCTCC 
                   
               
               
                   
                   
                 CTTCCCTTCCCCGAGCTCAAGCCGATCGATGCCAAGTCGGAGCT 
                   
               
               
                   
                   
                 CGCGAAGCTCCGTGGCCTCATCGACCTCGAGAAGGTCGTAGTC 
                   
               
               
                   
                   
                 ATGACCGGTTTACGCCGAGGTCGGACCGTTCGGCTCGTCGCGCA 
                   
               
               
                   
                   
                 CGCGCTGGGAGATGGAGGCGAACGGCACCTTCTCCATCCAGGG 
                   
               
               
                   
                   
                 CACACTCGAGCTTGCGTACGTCATGGGCCTCATCAAGCACTTTG 
                   
               
               
                   
                   
                 AGGGTCGCCTCAAGGACGGCACGCTCTACGTCGGATGGGTCGA 
                   
               
               
                   
                   
                 CGCCAAGACGAACGAACCGCTGGACGACAAGGACGTCAAGGC 
                   
               
               
                   
                   
                 TGCGTACGAGAAGCACATTCTCGCGCACACCGGCATCCGCCTC 
                   
               
               
                   
                   
                 ATCGAGCCGGAGATCTTCAACGGCTACGACCCGAAGCGCAAGG 
                   
               
               
                   
                   
                 GCTTCACGCAGGAGATCGAGATCCAGCACGACCTCGAGCCCAT 
                   
               
               
                   
                   
                 CGAGGCGTCCGAGGAGGACGCGGCTCGCTTCAAGCGCGAGCAC 
                   
               
               
                   
                   
                 GGCGCGCTCGTCGACGTCTACACCGAGGACGGCAGCAAGTTCT 
                   
               
               
                   
                   
                 TCGTCAAGTTCAAGAAGGGCGCCAAGCTGCACATTCCCAAGGC 
                   
               
               
                   
                   
                 TGTTGCCTTCGACCGCCTTGTCGCCGGACAGATCCCGACTGGCT 
                   
               
               
                   
                   
                 GGTCGCACAAGGCCTTCGGTATCCCCGACGACATTGCCTCGCA 
                   
               
               
                   
                   
                 GGTTGACCGCACCTCGCTGTGGGCGCTCGTCTCGGTCGCCGAG 
                   
               
               
                   
                   
                 GCGCTCATGATGGCCGGCATCACCGACCCGTATGAGCTCTACA 
                   
               
               
                   
                   
                 AGTGGATTCACCCGAGCGAGGTCGGTTCGTCGCTCGGATCCGG 
                   
               
               
                   
                   
                 CATGGGAGGCATCACGAGTATCTCGAAGATGTTCCGCGACCGC 
                   
               
               
                   
                   
                 CGCGAGGAGAAGGACGTCCAGAAGGACATCCTCCAGGAGACC 
                   
               
               
                   
                   
                 TTCATCAATACGGTCGCCGGATGGGTCAACCTCCTCCTTCTCTC 
                   
               
               
                   
                   
                 GTCATCCGGACCGATCAAGATCCCCGTCGGCGCCTGCGCGACT 
                   
               
               
                   
                   
                 GCCCTCCAGTCGGTCGAGATCGCCTGCGACACCATCCTCAGCG 
                   
               
               
                   
                   
                 GCAAGGCCAAGATCATGGTCTCGGGAGGCTACGACGACTTCTC 
                   
               
               
                   
                   
                 CGAGGAGGGCTCGTACGAGTTCGCAAACATGAAGGCGACCTCG 
                   
               
               
                   
                   
                 AACAGCGAGACCGAGTTCGCTGCCGGCCGCGAGCCGAACGAG 
                   
               
               
                   
                   
                 ATGTCGCGTCCGACGACCAGCACCCGTGCCGGCTTCATGGAGT 
                   
               
               
                   
                   
                 CGATGGGTTGCGGTGCTCAGGTCCTGATGTCGGCGAAGACGGC 
                   
               
               
                   
                   
                 CATCGAGATGGGCGCCACCATCTACGGCATCGTCGCCTACACC 
                   
               
               
                   
                   
                 GCGACCGCCACCGACAAGGCTGGTCGCTCGATTCCCGCCCCCG 
                   
               
               
                   
                   
                 GACGCGGTGTCATGGGTACCGCGCGCGAGATCACCTCCAAGTA 
                   
               
               
                   
                   
                 CCCCTCGCCCATCCTCGATGTCACCTACCGCCGCCGCCAGCTCG 
                   
               
               
                   
                   
                 AGTTCCGTCGCAAGCAGATCTCGCAGTGGCTCGAGAACGAGAC 
                   
               
               
                   
                   
                 CGAGCTCCTCAAGTTCGAGGTCTCCTCGCACGGACAGGCCACA 
                   
               
               
                   
                   
                 AAGCTCCCCGACGACTACGTCTCCGAGCGCCTCGCATCCATCG 
                   
               
               
                   
                   
                 AACGCGAAGCCAAGCGCCAGGAGGCCGAGGCTCTCGCGACGT 
                   
               
               
                   
                   
                 ACGGCATGCTCGCCGGCCAGGACCCGACCATCGCCCCGCTCCG 
                   
               
               
                   
                   
                 TCGCGCTCTCGCCGTTTGGGGTCTCACCATCGACGACGTTGGAG 
                   
               
               
                   
                   
                 TCGCCTCGTTCCACGGCACCTCGACCGTTGCCAACGACAAGAA 
                   
               
               
                   
                   
                 CGAGTCGAACGCGTACAACGAGCAGTTCCGTCACCTTGGCCGC 
                   
               
               
                   
                   
                 GCCAAGGGTAACGCCTGCCCCGTCATCGCTCAGAAGTGGCTCA 
                   
               
               
                   
                   
                 CCGGACACCCGAAGGGAGGTGCCGCCGCCTGGATGCTCAACGG 
                   
               
               
                   
                   
                 CTTGGCCCAGGTCATTCAGAGCGGTCTCGTTCCCGGCAACCGC 
                   
               
               
                   
                   
                 AACGCCGACAACATCGGCGAAGAGCTTCGCGCGTTCGAGTACC 
                   
               
               
                   
                   
                 TGCTCTACCCGTCCAAGTCGATCCAGACCGACGGCATCAAGGC 
                   
               
               
                   
                   
                 TGGTCTCCTCACCTCGTTCGGCTTCGGTCAAGTCGGTGGCCAGG 
                   
               
               
                   
                   
                 CTCTCATCGTTCACCCGAGTCTGCTCATCGGCGCGCTCGAGCCC 
                   
               
               
                   
                   
                 GCCCAGTTCGAGGCGTACAAGAAGCTCAACGACCAGCGCAAG 
                   
               
               
                   
                   
                 AAGTGGTCATACCGTCGCTTCAACGATTTCTTCACGAACGGCA 
                   
               
               
                   
                   
                 AGCTCGTCATTATCAAGGACGGCACGCCCTTCACGCCCGAGCA 
                   
               
               
                   
                   
                 GGAGAACACGACCCTCCTCAACCCGCTCGTCCGCGCCGTGCCC 
                   
               
               
                   
                   
                 GACAAGACTGGCTCGTACTCGATGCCGAAGGAGTTCCCTGCCA 
                   
               
               
                   
                   
                 CCGTCCCTCGCAGCAACAACGCCGAAGTCGCCAACAAGCTCGT 
                   
               
               
                   
                   
                 CAGCGCGGCTGTCGGCGGTGCTTTCGGCGTCGGCACGGACGTC 
                   
               
               
                   
                   
                 GAGCTGATCAGCGCCGTCCCGACCTCGGAGTCGTTCCTCGAGA 
                   
               
               
                   
                   
                 GGAACTTCACCCAGGACGAGATCGCCTACTGCAAGGCCGCACC 
                   
               
               
                   
                   
                 CGACTTCCGCGCTAGCCTCGCCGCGCGCTGGTCCGCCAAGGAG 
                   
               
               
                   
                   
                 GCCACTTTCAAGGCTCTCAAGACCGAGTCGAAGGGCGCCGCCG 
                   
               
               
                   
                   
                 CCAGCATGCAGGACATCGAGGTCGTCTCCACGTCGCAGGGCCC 
                   
               
               
                   
                   
                 GACTATCAAGCTCCACGGCGAGGTCGAGAAGATCGCCCAGGCC 
                   
               
               
                   
                   
                 GCCGGCATCACGGCCTTCGAGGTCTCGCTCTCGCACTCGGAGG 
                   
               
               
                   
                   
                 ACGTCGCTTGCGCCGTCGTCATCGCCCAGAAGTAG 
                   
               
               
                   
               
               
                 22 
                 Acr1 
                 GGATCCAAAACAATGAATAAGAAGTTAGAAGCATTGTTTAGAGA 
                 177 
               
               
                   
                   
                 AAATGTCAAGGGTAAAGTCGCTTTAATCACTGGTGCCTCCTCAGG 
                   
               
               
                   
                   
                 TATCGGTTTAACTATCGCAAAAAGAATTGCTGCAGCCGGTGCCC 
                   
               
               
                   
                   
                 ATGTTTTGTTAGTCGCTAGAACTCAAGAAACATTGGAAGAAGTT 
                   
               
               
                   
                   
                 AAGGCTGCAATCGAACAACAAGGTGGTCAAGCATCTATATTCCC 
                   
               
               
                   
                   
                 ATGTGATTTGACAGACATGAATGCAATAGATCAATTATCCCAAC 
                   
               
               
                   
                   
                 AAATCATGGCCAGTGTAGATCATGTTGACTTTTTGATTAATAACG 
                   
               
               
                   
                   
                 CAGGTAGATCTATAAGAAGAGCCGTTCATGAATCATTTGATAGA 
                   
               
               
                   
                   
                 TTCCACGACTTCGAAAGAACAATGCAATTAAACTACTTCGGTGCT 
                   
               
               
                   
                   
                 GTCAGATTGGTATTGAACTTGTTGCCTCACATGATCAAGAGAAA 
                   
               
               
                   
                   
                 GAATGGTCAAATTATAAACATCTCTTCAATCGGTGTATTGGCCAA 
                   
               
               
                   
                   
                 CGCTACCAGATTCTCTGCTTATGTTGCATCAAAAGCCGCTTTAGA 
                   
               
               
                   
                   
                 TGCTTTTTCCAGATGCTTGAGTGCAGAAGTTTTGAAGCATAAGAT 
                   
               
               
                   
                   
                 CTCTATAACTTCAATCTATATGCCATTGGTCAGAACACCAATGAT 
                   
               
               
                   
                   
                 CGCACCTACCAAAATCTATAAGTACGTTCCAACATTGTCTCCTGA 
                   
               
               
                   
                   
                 AGAAGCAGCCGATTTGATAGTTTATGCTATCGTCAAGAGACCTA 
                   
               
               
                   
                   
                 CCAGAATTGCCACTCACTTGGGTAGATTAGCTTCCATTACCTACG 
                   
               
               
                   
                   
                 CAATAGCCCCAGACATAAACAACATCTTGATGTCTATTGGTTTTA 
                   
               
               
                   
                   
                 ATTTGTTTCCTTCCAGTACTGCTGCATTAGGTGAACAAGAAAAAT 
                   
               
               
                   
                   
                 TGAACTTATTACAAAGAGCCTACGCAAGATTATTCCCTGGTGAAC 
                   
               
               
                   
                   
                 ATTGGTGAAAGCTT 
                   
               
               
                   
               
               
                 47 
                 ACB1 
                 ATGGTTTCCCAATTATTCGAAGAAAAAGCTAAAGCCGTCAACGA 
                 178 
               
               
                   
                   
                 GCTACCAACGAAGCCCTCCACTGATGAATTATTAGAATTGTATGC 
                   
               
               
                   
                   
                 TCTGTACAAGCAAGCCACTGTAGGTGACAACGACAAGGAAAAGC 
                   
               
               
                   
                   
                 CTGGTATTTTCAACATGAAGGACCGCTACAAGTGGGAAGCCTGG 
                   
               
               
                   
                   
                 GAAAACTTAAAAGGTAAATCCCAGGAAGATGCCGAAAAGGAAT 
                   
               
               
                   
                   
                 ACATTGCCCTTGTTGATCAACTGATTGCCAAGTACTCCTCTTAG 
                   
               
               
                   
               
               
                 48 
                 FOX2 
                 ATGCCTGGAAATTTATCCTTCAAAGATAGAGTTGTTGTAATCACG 
                 179 
               
               
                   
                   
                 GGCGCTGGAGGGGGCTTAGGTAAGGTGTATGCACTAGCTTACGC 
                   
               
               
                   
                   
                 AAGCAGAGGTGCAAAAGTGGTCGTCAATGATCTAGGTGGCACTT 
                   
               
               
                   
                   
                 TGGGTGGTTCAGGACATAACTCCAAAGCTGCAGACTTAGTGGTG 
                   
               
               
                   
                   
                 GATGAGATAAAAAAAGCCGGAGGTATAGCTGTGGCAAATTACGA 
                   
               
               
                   
                   
                 CTCTGTTAATGAAAATGGAGAGAAAATAATTGAAACGGCTATAA 
                   
               
               
                   
                   
                 AAGAATTCGGCAGGGTTGATGTACTAATTAACAACGCTGGAATA 
                   
               
               
                   
                   
                 TTAAGGGATGTTTCATTTGCAAAGATGACAGAACGTGAGTTTGC 
                   
               
               
                   
                   
                 ATCTGTGGTAGATGTTCATTTGACAGGTGGCTATAAGCTATCGCG 
                   
               
               
                   
                   
                 TGCTGCTTGGCCTTATATGCGCTCTCAGAAATTTGGTAGAATCAT 
                   
               
               
                   
                   
                 TAACACCGCTTCCCCTGCCGGTCTATTTGGAAATTTTGGTCAAGC 
                   
               
               
                   
                   
                 TAATTATTCAGCAGCTAAAATGGGCTTAGTTGGTTTGGCGGAAAC 
                   
               
               
                   
                   
                 CCTCGCGAAGGAGGGTGCCAAATACAACATTAATGTTAATTCAA 
                   
               
               
                   
                   
                 TTGCGCCATTGGCTAGATCACGTATGACAGAAAACGTGTTACCA 
                   
               
               
                   
                   
                 CCACATATCTTGAAACAGTTAGGACCGGAAAAAATTGTTCCCTTA 
                   
               
               
                   
                   
                 GTACTCTATTTGACACACGAAAGTACGAAAGTGTCAAACTCCATT 
                   
               
               
                   
                   
                 TTTGAACTCGCTGCTGGATTCTTTGGACAGCTCAGATGGGAGAGG 
                   
               
               
                   
                   
                 TCTTCTGGACAAATTTTCAATCCAGACCCCAAGACATATACTCCT 
                   
               
               
                   
                   
                 GAAGCAATTTTAAATAAGTGGAAGGAAATCACAGACTATAGGGA 
                   
               
               
                   
                   
                 CAAGCCATTTAACAAAACTCAGCATCCATATCAACTCTCGGATTA 
                   
               
               
                   
                   
                 TAATGATTTAATCACCAAAGCAAAAAAATTACCTCCCAATGAAC 
                   
               
               
                   
                   
                 AAGGCTCAGTGAAAATCAAGTCGCTTTGCAACAAAGTCGTAGTA 
                   
               
               
                   
                   
                 GTTACGGGTGCAGGAGGTGGTCTTGGGAAGTCTCATGCAATCTG 
                   
               
               
                   
                   
                 GTTTGCACGGTACGGTGCGAAGGTAGTTGTAAATGACATCAAGG 
                   
               
               
                   
                   
                 ATCCTTTTTCAGTTGTTGAAGAAATAAATAAACTATATGGTGAAG 
                   
               
               
                   
                   
                 GCACAGCCATTCCAGATTCCCATGATGTGGTCACCGAAGCTCCTC 
                   
               
               
                   
                   
                 TCATTATCCAAACTGCAATAAGTAAGTTTCAGAGAGTAGACATCT 
                   
               
               
                   
                   
                 TGGTCAATAACGCTGGTATTTTGCGTGACAAATCTTTTTTAAAAA 
                   
               
               
                   
                   
                 TGAAAGATGAGGAATGGTTTGCTGTCCTGAAAGTCCACCTTTTTT 
                   
               
               
                   
                   
                 CCACATTTTCATTGTCAAAAGCAGTATGGCCAATATTTACCAAAC 
                   
               
               
                   
                   
                 AAAAGTCTGGATTTATTATCAATACTACTTCTACCTCAGGAATTT 
                   
               
               
                   
                   
                 ATGGTAATTTTGGACAGGCCAATTATGCCGCTGCAAAAGCCGCC 
                   
               
               
                   
                   
                 ATTTTAGGATTCAGTAAAACTATTGCACTGGAAGGTGCCAAGAG 
                   
               
               
                   
                   
                 AGGAATTATTGTTAATGTTATCGCTCCTCATGCAGAAACGGCTAT 
                   
               
               
                   
                   
                 GACAAAGACTATATTCTCGGAGAAGGAATTATCAAACCACTTTG 
                   
               
               
                   
                   
                 ATGCATCTCAAGTCTCCCCACTTGTTGTTTTGTTGGCATCTGAAG 
                   
               
               
                   
                   
                 AACTACAAAAGTATTCTGGAAGAAGGGTTATTGGCCAATTATTC 
                   
               
               
                   
                   
                 GAAGTTGGCGGTGGTTGGTGTGGGCAAACCAGATGGCAAAGAAG 
                   
               
               
                   
                   
                 TTCCGGTTATGTTTCTATTAAAGAGACTATTGAACCGGAAGAAAT 
                   
               
               
                   
                   
                 TAAAGAAAATTGGAACCACATCACTGATTTCAGTCGCAACACTA 
                   
               
               
                   
                   
                 TCAACCCGAGCTCCACAGAGGAGTCTTCTATGGCAACCTTGCAA 
                   
               
               
                   
                   
                 GCCGTGCAAAAAGCGCACTCTTCAAAGGAGTTGGATGATGGATT 
                   
               
               
                   
                   
                 ATTCAAGTACACTACCAAGGATTGTATCTTGTACAATTTAGGACT 
                   
               
               
                   
                   
                 TGGATGCACAAGCAAAGAGCTTAAGTACACCTACGAGAATGATC 
                   
               
               
                   
                   
                 CAGACTTCCAAGTTTTGCCCACGTTCGCCGTCATTCCATTTATGC 
                   
               
               
                   
                   
                 AAGCTACTGCCACACTAGCTATGGACAATTTAGTCGATAACTTCA 
                   
               
               
                   
                   
                 ATTATGCAATGTTACTGCATGGAGAACAATATTTTAAGCTCTGCA 
                   
               
               
                   
                   
                 CGCCGACAATGCCAAGTAATGGAACTCTAAAGACACTTGCTAAA 
                   
               
               
                   
                   
                 CCTTTACAAGTACTTGACAAGAATGGTAAAGCCGCTTTAGTTGTT 
                   
               
               
                   
                   
                 GGTGGCTTCGAAACTTATGACATTAAAACTAAGAAACTCATAGC 
                   
               
               
                   
                   
                 TTATAACGAAGGATCGTTCTTCATCAGGGGCGCACATGTACCTCC 
                   
               
               
                   
                   
                 AGAAAAGGAAGTGAGGGATGGGAAAAGAGCCAAGTTTGCTGTC 
                   
               
               
                   
                   
                 CAAAATTTTGAAGTGCCACATGGAAAGGTACCAGATTTTGAGGC 
                   
               
               
                   
                   
                 GGAGATTTCTACGAATAAAGATCAAGCCGCATTGTACAGGTTAT 
                   
               
               
                   
                   
                 CTGGCGATTTCAATCCTTTACATATCGATCCCACGCTAGCCAAAG 
                   
               
               
                   
                   
                 CAGTTAAATTTCCTACGCCAATTCTGCATGGGCTTTGTACATTAG 
                   
               
               
                   
                   
                 GTATTAGTGCGAAAGCATTGTTTGAACATTATGGTCCATATGAGG 
                   
               
               
                   
                   
                 AGTTGAAAGTGAGATTTACCAATGTTGTTTTCCCAGGTGATACTC 
                   
               
               
                   
                   
                 TAAAGGTTAAAGCTTGGAAGCAAGGCTCGGTTGTCGTTTTTCAAA 
                   
               
               
                   
                   
                 CAATTGATACGACCAGAAACGTCATTGTATTGGATAACGCCGCT 
                   
               
               
                   
                   
                 GTAAAACTATCGCAGGCAAAATAA 
                   
               
               
                   
               
               
                 49 
                 FOX3 
                 ATGGGTAAGGGTGAATCGAAGAGGAAGAACTCGTTGCTGGAGA 
                 180 
               
               
                   
                   
                 AAAGACCCGAAGATGTAGTTATTGTGGCTGCTAACAGGTCTGCC 
                   
               
               
                   
                   
                 ATCGGTAAAGGTTTTAAAGGTGCCTTCAAAGATGTAAACACAGA 
                   
               
               
                   
                   
                 CTACTTATTATACAACTTTCTCAATGAGTTCATCGGGAGGTTTCC 
                   
               
               
                   
                   
                 GGAACCTTTGAGGGCTGATTTGAACTTAATCGAAGAAGTTGCCT 
                   
               
               
                   
                   
                 GTGGAAATGTTCTCAATGTTGGAGCCGGTGCTACAGAACACAGG 
                   
               
               
                   
                   
                 GCTGCATGCTTGGCAAGTGGGATTCCCTACTCGACGCCATTTGTC 
                   
               
               
                   
                   
                 GCTTTAAACAGACAATGTTCTTCAGGTTTAACGGCGGTGAACGAT 
                   
               
               
                   
                   
                 ATTGCCAACAAGATTAAGGTTGGGCAAATTGATATTGGTTTGGC 
                   
               
               
                   
                   
                 GCTGGGAGTGGAATCAATGACCAATAACTACAAAAACGTCAATC 
                   
               
               
                   
                   
                 CCTTGGGCATGATCTCCTCTGAAGAGCTGCAAAAAAACCGAGAA 
                   
               
               
                   
                   
                 GCGAAGAAATGTCTAATACCAATGGGCATTACTAATGAGAATGT 
                   
               
               
                   
                   
                 TGCCGCTAATTTCAAGATCAGTAGAAAGGATCAAGACGAGTTCG 
                   
               
               
                   
                   
                 CTGCGAATTCATATCAAAAAGCTTACAAGGCGAAAAATGAGGGG 
                   
               
               
                   
                   
                 CTTTTCGAAGATGAAATTTTACCTATAAAATTACCAGATGGCTCA 
                   
               
               
                   
                   
                 ATTTGCCAGTCGGACGAAGGGCCACGCCCTAACGTCACTGCGGA 
                   
               
               
                   
                   
                 GTCGCTTTCAAGCATCAGGCCTGCCTTTATCAAAGACAGAGGAA 
                   
               
               
                   
                   
                 CCACAACTGCGGGCAATGCATCCCAGGTCTCCGATGGTGTGGCA 
                   
               
               
                   
                   
                 GGTGTCTTGTTAGCCCGCAGGTCCGTAGCCAACCAGTTAAATCTG 
                   
               
               
                   
                   
                 CCTGTGCTAGGTCGCTACATCGATTTTCAAACAGTGGGGGTTCCC 
                   
               
               
                   
                   
                 CCTGAAATCATGGGTGTGGGCCCTGCATACGCCATACCAAAAGT 
                   
               
               
                   
                   
                 CCTGGAAGCTACTGGCTTGCAAGTCCAAGATATCGATATTTTTGA 
                   
               
               
                   
                   
                 AATAAATGAAGCATTCGCGGCCCAAGCATTATACTGCATCCATA 
                   
               
               
                   
                   
                 AACTGGGCATCGATTTGAATAAAGTAAATCCAAGAGGTGGTGCA 
                   
               
               
                   
                   
                 ATCGCGTTAGGCCATCCCTTGGGTTGTACTGGCGCAAGGCAAGT 
                   
               
               
                   
                   
                 AGCTACCATACTAAGAGAACTGAAAAAGGATCAAATCGGGGTTG 
                   
               
               
                   
                   
                 TTAGTATGTGTATCGGTACTGGTATGGGTGCCGCCGCCATCTTTA 
                   
               
               
                   
                   
                 TTAAAGAATAG 
                   
               
               
                   
               
               
                 50 
                 ERG10 
                 ATGTCTCAGAACGTTTACATTGTATCGACTGCCAGAACCCCAATT 
                 181 
               
               
                   
                   
                 GGTTCATTCCAGGGTTCTCTATCCTCCAAGACAGCAGTGGAATTG 
                   
               
               
                   
                   
                 GGTGCTGTTGCTTTAAAAGGCGCCTTGGCTAAGGTTCCAGAATTG 
                   
               
               
                   
                   
                 GATGCATCCAAGGATTTTGACGAAATTATTTTTGGTAACGTTCTT 
                   
               
               
                   
                   
                 TCTGCCAATTTGGGCCAAGCTCCGGCCAGACAAGTTGCTTTGGCT 
                   
               
               
                   
                   
                 GCCGGTTTGAGTAATCATATCGTTGCAAGCACAGTTAACAAGGT 
                   
               
               
                   
                   
                 CTGTGCATCCGCTATGAAGGCAATCATTTTGGGTGCTCAATCCAT 
                   
               
               
                   
                   
                 CAAATGTGGTAATGCTGATGTTGTCGTAGCTGGTGGTTGTGAATC 
                   
               
               
                   
                   
                 TATGACTAACGCACCATACTACATGCCAGCAGCCCGTGCGGGTG 
                   
               
               
                   
                   
                 CCAAATTTGGCCAAACTGTTCTTGTTGATGGTGTCGAAAGAGATG 
                   
               
               
                   
                   
                 GGTTGAACGATGCGTACGATGGTCTAGCCATGGGTGTACACGCA 
                   
               
               
                   
                   
                 GAAAAGTGTGCCCGTGATTGGGATATTACTAGAGAACAACAAGA 
                   
               
               
                   
                   
                 CAATTTTGCCATCGAATCCTACCAAAAATCTCAAAAATCTCAAAA 
                   
               
               
                   
                   
                 GGAAGGTAAATTCGACAATGAAATTGTACCTGTTACCATTAAGG 
                   
               
               
                   
                   
                 GATTTAGAGGTAAGCCTGATACTCAAGTCACGAAGGACGAGGAA 
                   
               
               
                   
                   
                 CCTGCTAGATTACACGTTGAAAAATTGAGATCTGCAAGGACTGTT 
                   
               
               
                   
                   
                 TTCCAAAAAGAAAACGGTACTGTTACTGCCGCTAACGCTTCTCCA 
                   
               
               
                   
                   
                 ATCAACGATGGTGCTGCAGCCGTCATCTTGGTTTCCGAAAAAGTT 
                   
               
               
                   
                   
                 TTGAAGGAAAAGAATTTGAAGCCTTTGGCTATTATCAAAGGTTG 
                   
               
               
                   
                   
                 GGGTGAGGCCGCTCATCAACCAGCTGATTTTACATGGGCTCCATC 
                   
               
               
                   
                   
                 TCTTGCAGTTCCAAAGGCTTTGAAACATGCTGGCATCGAAGACAT 
                   
               
               
                   
                   
                 CAATTCTGTTGATTACTTTGAATTCAATGAAGCCTTTTCGGTTGTC 
                   
               
               
                   
                   
                 GGTTTGGTGAACACTAAGATTTTGAAGCTAGACCCATCTAAGGTT 
                   
               
               
                   
                   
                 AATGTATATGGTGGTGCTGTTGCTCTAGGTCACCCATTGGGTTGT 
                   
               
               
                   
                   
                 TCTGGTGCTAGAGTGGTTGTTACACTGCTATCCATCTTACAGCAA 
                   
               
               
                   
                   
                 GAAGGAGGTAAGATCGGTGTTGCCGCCATTTGTAATGGTTGA 
                   
               
               
                   
               
               
                 51 
                 TES1 
                 ATGAGTGCTTCCAAAATGGCCATGTCCAACCTAGAGAAAATATT 
                 182 
               
               
                   
                   
                 GGAACTGGTTCCTCTTTCGCCTACCAGTTTTGTCACAAAGTATCT 
                   
               
               
                   
                   
                 GCCTGCCGCGCCCGTAGGGTCTAAGGGCACTTTTGGTGGAACGC 
                   
               
               
                   
                   
                 TGGTATCACAATCGCTGCTGGCGTCATTGCATACTGTGCCATTGA 
                   
               
               
                   
                   
                 ACTTCTTCCCCACATCGCTACATTCGTATTTCATCAAGGGTGGTG 
                   
               
               
                   
                   
                 ATCCGCGGACCAAGATCACGTACCATGTGCAGAATCTGAGAAAC 
                   
               
               
                   
                   
                 GGTAGAAATTTCATCCATAAGCAGGTTAGTGCTTATCAGCACGA 
                   
               
               
                   
                   
                 CAAGTTGATATTTACGTCGATGATCTTATTTGCCGTGCAACGGTC 
                   
               
               
                   
                   
                 CAAGGAGCACGACTCCTTGCAGCACTGGGAGACGATTCCAGGCC 
                   
               
               
                   
                   
                 TGCAAGGTAAGCAGCCAGACCCTCATCGTTATGAAGAGGCCACT 
                   
               
               
                   
                   
                 TCGCTTTTCCAGAAAGAAGTTCTGGACCCACAGAAATTGAGCAG 
                   
               
               
                   
                   
                 GTATGCCTCATTGTCCGACAGGTTCCAAGACGCAACCTCGATGA 
                   
               
               
                   
                   
                 GCAAGTATGTGGATGCGTTTCAATACGGAGTCATGGAGTACCAA 
                   
               
               
                   
                   
                 TTCCCCAAGGACATGTTCTACTCGGCAAGACACACCGACGAGCT 
                   
               
               
                   
                   
                 GGATTATTTCGTCAAAGTGAGACCTCCCATCACTACCGTGGAGCA 
                   
               
               
                   
                   
                 CGCGGGCGACGAGTCTTCTTTACACAAGCATCATCCGTACAGGA 
                   
               
               
                   
                   
                 TCCCGAAGAGCATTACTCCTGAGAACGACGCTCGCTACAACTAC 
                   
               
               
                   
                   
                 GTGGCCTTTGCGTATCTGTCCGATTCCTACCTCCTACTCACGATCC 
                   
               
               
                   
                   
                 CGTACTTCCACAACCTGCCTTTGTACTGCCACAGTTTCAGTGTCT 
                   
               
               
                   
                   
                 CGCTCGACCACACGATTTACTTTCACCAGTTGCCTCATGTGAACA 
                   
               
               
                   
                   
                 ATTGGATCTATCTTAAGATTTCGAATCCCAGGTCCCACTGGGACA 
                   
               
               
                   
                   
                 AGCACCTCGTACAGGGCAAGTATTTCGACACACAGTCGGGACGC 
                   
               
               
                   
                   
                 ATCATGGCAAGCGTCTCTCAGGAGGGCTACGTTGTCTACGGGTC 
                   
               
               
                   
                   
                 AGAACGAGACATTCGATGA 
                   
               
               
                   
               
               
                 52 
                 FadA 
                 ATGGAACAAGTAGTAATCGTAGACGCAATCAGAACTCCTATGGG 
                 183 
               
               
                   
                   
                 TAGAAGTAAAGGTGGTGCATTCAGAAATGTCAGAGCAGAAGACT 
                   
               
               
                   
                   
                 TGTCCGCTCATTTGATGAGAAGTTTGTTAGCAAGAAATCCAGCCT 
                   
               
               
                   
                   
                 TGGAAGCTGCAGCCTTAGATGACATCTATTGGGGTTGTGTTCAAC 
                   
               
               
                   
                   
                 AAACTTTGGAACAAGGTTTTAATATCGCAAGAAACGCTGCATTG 
                   
               
               
                   
                   
                 TTAGCCGAAGTTCCACATTCTGTCCCTGCTGTAACCGTTAACAGA 
                   
               
               
                   
                   
                 TTGTGTGGTTCTTCAATGCAAGCATTACACGATGCCGCTAGAATG 
                   
               
               
                   
                   
                 ATTATGACTGGTGACGCCCAAGCTTGCTTGGTCGGTGGTGTAGAA 
                   
               
               
                   
                   
                 CATATGGGTCACGTCCCAATGTCCCATGGTGTAGATTTCCACCCT 
                   
               
               
                   
                   
                 GGTTTAAGTAGAAATGTTGCTAAAGCAGCCGGTATGATGGGTTT 
                   
               
               
                   
                   
                 GACAGCTGAAATGTTAGCAAGAATGCATGGTATTTCTAGAGAAA 
                   
               
               
                   
                   
                 TGCAAGATGCATTTGCTGCAAGATCTCACGCAAGAGCCTGGGCC 
                   
               
               
                   
                   
                 GCTACTCAATCAGCAGCCTTCAAAAATGAAATTATACCAACAGG 
                   
               
               
                   
                   
                 TGGTCATGATGCTGACGGTGTTTTGAAGCAATTCAATTACGATGA 
                   
               
               
                   
                   
                 AGTTATAAGACCTGAAACTACAGTCGAAGCTTTGGCAACCTTAA 
                   
               
               
                   
                   
                 GACCAGCATTCGATCCTGTAAATGGTATGGTTACAGCTGGTACCT 
                   
               
               
                   
                   
                 CCAGTGCATTGTCCGACGGTGCTGCAGCCATGTTAGTAATGTCTG 
                   
               
               
                   
                   
                 AATCAAGAGCTCACGAATTGGGTTTAAAACCAAGAGCCAGAGTT 
                   
               
               
                   
                   
                 AGATCTATGGCTGTTGTCGGTTGCGATCCTTCAATAATGGGTTAC 
                   
               
               
                   
                   
                 GGTCCAGTCCCTGCCTCAAAGTTGGCTTTGAAGAAAGCAGGTTTG 
                   
               
               
                   
                   
                 TCCGCCAGTGACATCGGTGTTTTTGAAATGAATGAAGCTTTCGCT 
                   
               
               
                   
                   
                 GCACAAATATTGCCATGTATCAAGGATTTGGGTTTGATCGAACA 
                   
               
               
                   
                   
                 AATAGACGAAAAGATTAATTTGAACGGTGGTGCCATAGCTTTGG 
                   
               
               
                   
                   
                 GTCATCCTTTAGGTTGCTCTGGTGCTAGAATCTCAACCACTTTGTT 
                   
               
               
                   
                   
                 GAACTTAATGGAAAGAAAGGATGTTCAATTTGGTTTGGCAACTA 
                   
               
               
                   
                   
                 TGTGTATCGGTTTAGGTCAAGGTATCGCTACTGTATTTGAAAGAG 
                   
               
               
                   
                   
                 TCTAA 
                   
               
               
                   
               
               
                 53 
                 FadB 
                 ATGTTGTATAAAGGTGACACATTGTACTTAGACTGGTTAGAAGAT 
                 184 
               
               
                   
                   
                 GGTATCGCTGAATTGGTATTTGATGCTCCTGGTTCCGTAAACAAA 
                   
               
               
                   
                   
                 TTGGATACTGCCACAGTAGCTTCCTTAGGTGAAGCAATTGGTGTT 
                   
               
               
                   
                   
                 TTGGAACAACAATCCGACTTAAAGGGTTTGTTGTTGAGAAGTAA 
                   
               
               
                   
                   
                 TAAGGCTGCTTTTATTGTAGGTGCTGATATCACAGAATTCTTGAG 
                   
               
               
                   
                   
                 TTTGTTTTTAGTTCCAGAAGAACAATTGTCTCAATGGTTGCATTTC 
                   
               
               
                   
                   
                 GCAAACTCAGTTTTTAACAGATTGGAAGATTTGCCAGTCCCTACC 
                   
               
               
                   
                   
                 ATTGCCGCTGTAAACGGTTACGCTTTAGGTGGTGGTTGTGAATGC 
                   
               
               
                   
                   
                 GTTTTGGCTACCGACTATAGATTAGCAACTCCAGATTTGAGAATC 
                   
               
               
                   
                   
                 GGTTTACCTGAAACTAAATTGGGTATTATGCCAGGTTTTGGTGGT 
                   
               
               
                   
                   
                 TCTGTTAGAATGCCTAGAATGTTGGGTGCAGATTCAGCCTTAGAA 
                   
               
               
                   
                   
                 ATTATAGCAGCCGGTAAAGACGTTGGTGCTGATCAAGCATTGAA 
                   
               
               
                   
                   
                 GATCGGTTTAGTCGATGGTGTTGTCAAAGCTGAAAAGTTGGTTGA 
                   
               
               
                   
                   
                 AGGTGCCAAAGCTGTCTTAAGACAAGCCATTAATGGTGACTTGG 
                   
               
               
                   
                   
                 ACTGGAAAGCTAAGAGACAACCAAAGTTAGAACCTTTGAAGTTG 
                   
               
               
                   
                   
                 TCTAAGATCGAAGCAACAATGTCTTTTACTATAGCCAAGGGTATG 
                   
               
               
                   
                   
                 GTCGCCCAAACTGCTGGTAAACATTACCCAGCCCCTATAACTGCT 
                   
               
               
                   
                   
                 GTTAAAACAATCGAAGCTGCAGCCAGATTCGGTAGAGAAGAAGC 
                   
               
               
                   
                   
                 ATTGAATTTGGAAAACAAGTCTTTTGTTCCATTGGCTCACACAAA 
                   
               
               
                   
                   
                 TGAAGCAAGAGCCTTGGTCGGTATTTTCTTGAACGACCAATACGT 
                   
               
               
                   
                   
                 AAAGGGTAAAGCTAAGAAATTGACTAAAGATGTTGAAACACCAA 
                   
               
               
                   
                   
                 AGCAAGCTGCAGTCTTGGGTGCTGGTATCATGGGTGGTGGTATTG 
                   
               
               
                   
                   
                 CATATCAATCCGCCTGGAAAGGTGTTCCTGTAGTTATGAAGGATA 
                   
               
               
                   
                   
                 TCAACGACAAGAGTTTGACCTTGGGTATGACTGAAGCCGCTAAG 
                   
               
               
                   
                   
                 TTGTTGAACAAGCAATTAGAAAGAGGTAAAATTGACGGTTTGAA 
                   
               
               
                   
                   
                 GTTAGCTGGTGTTATATCTACAATCCATCCAACCTTGGATTATGC 
                   
               
               
                   
                   
                 TGGTTTCGATAGAGTTGACATTGTCGTAGAAGCAGTTGTCGAAA 
                   
               
               
                   
                   
                 ATCCTAAAGTTAAAAAGGCAGTCTTAGCCGAAACAGAACAAAAA 
                   
               
               
                   
                   
                 GTTAGACAAGATACCGTTTTGGCTTCCAACACCAGTACTATCCCA 
                   
               
               
                   
                   
                 ATTTCAGAATTGGCTAATGCATTAGAAAGACCTGAAAACTTCTGT 
                   
               
               
                   
                   
                 GGTATGCATTTCTTTAATCCAGTACACAGAATGCCTTTGGTTGAA 
                   
               
               
                   
                   
                 ATCATAAGAGGTGAAAAATCTTCAGATGAAACTATCGCTAAGGT 
                   
               
               
                   
                   
                 AGTTGCCTGGGCTTCTAAAATGGGTAAAACACCAATCGTCGTAA 
                   
               
               
                   
                   
                 ATGATTGCCCTGGTTTCTTTGTCAACAGAGTATTGTTTCCATACTT 
                   
               
               
                   
                   
                 CGCAGGTTTTTCACAATTATTGAGAGATGGTGCCGACTTCAGAAA 
                   
               
               
                   
                   
                 GATAGATAAGGTTATGGAAAAGCAATTTGGTTGGCCAATGGGTC 
                   
               
               
                   
                   
                 CTGCCTATTTGTTGGACGTTGTCGGTATAGATACAGCTCATCACG 
                   
               
               
                   
                   
                 CACAAGCCGTTATGGCAGCCGGTTTCCCACAAAGAATGCAAAAA 
                   
               
               
                   
                   
                 GATTACAGAGACGCTATTGATGCATTATTCGACGCTAATAGATTT 
                   
               
               
                   
                   
                 GGTCAAAAGAATGGTTTGGGTTTTTGGAGATATAAGGAAGATTC 
                   
               
               
                   
                   
                 CAAAGGTAAACCTAAAAAGGAAGAAGACGCTGCAGTCGAAGAT 
                   
               
               
                   
                   
                 TTGTTGGCAGAAGTATCCCAACCAAAGAGAGATTTCAGTGAAGA 
                   
               
               
                   
                   
                 AGAAATCATCGCTAGAATGATGATTCCTATGGTCAACGAAGTAG 
                   
               
               
                   
                   
                 TTAGATGTTTAGAAGAAGGTATCATCGCTACCCCAGCTGAAGCA 
                   
               
               
                   
                   
                 GATATGGCATTGGTTTACGGTTTAGGTTTCCCACCTTTTCACGGT 
                   
               
               
                   
                   
                 GGTGCTTTTAGATGGTTGGACACTTTAGGTTCTGCCAAATATTTG 
                   
               
               
                   
                   
                 GATATGGCTCAACAATACCAACATTTGGGTCCATTATATGAAGTT 
                   
               
               
                   
                   
                 CCTGAAGGTTTGAGAAACAAGGCTAGACACAATGAACCTTATTA 
                   
               
               
                   
                   
                 CCCTCCTGTTGAACCTGCCAGACCTGTTGGTGACTTGAAAACTGC 
                   
               
               
                   
                   
                 CTAA 
                   
               
               
                   
               
               
                 54 
                 yqeF 
                 ATGAAGGATGTCGTAATCGTTGGTGCTTTAAGAACCCCTATCGGT 
                 185 
               
               
                   
                   
                 TGCTTTAGAGGTGCATTGGCTGGTCACTCCGCTGTAGAATTGGGT 
                   
               
               
                   
                   
                 TCTTTGGTTGTCAAAGCTTTAATAGAAAGAACTGGTGTACCAGCA 
                   
               
               
                   
                   
                 TATGCCGTCGATGAAGTAATCTTGGGTCAAGTTTTAACAGCTGGT 
                   
               
               
                   
                   
                 GCAGGTCAAAATCCAGCAAGACAATCAGCCATCAAAGGTGGTTT 
                   
               
               
                   
                   
                 GCCTAACTCTGTTTCAGCTATAACTATTAATGACGTCTGTGGTTC 
                   
               
               
                   
                   
                 TGGTTTAAAGGCATTGCATTTGGCAACCCAAGCCATTCAATGCGG 
                   
               
               
                   
                   
                 TGAAGCAGATATCGTCATTGCCGGTGGTCAAGAAAACATGTCAA 
                   
               
               
                   
                   
                 GAGCCCCTCACGTATTGACTGACTCCAGAACAGGTGCACAATTG 
                   
               
               
                   
                   
                 GGTAACTCACAATTGGTAGATTCCTTAGTTCATGATGGTTTGTGG 
                   
               
               
                   
                   
                 GACGCTTTTAATGATTACCACATCGGTGTTACTGCTGAAAACTTA 
                   
               
               
                   
                   
                 GCAAGAGAATACGGTATTTCAAGACAATTGCAAGATGCCTACGC 
                   
               
               
                   
                   
                 TTTATCTTCACAACAAAAAGCTAGAGCTGCAATTGACGCAGGTA 
                   
               
               
                   
                   
                 GATTCAAAGATGAAATAGTCCCAGTAATGACCCAAAGTAATGGT 
                   
               
               
                   
                   
                 CAAACCTTGGTAGTTGATACTGACGAACAACCAAGAACTGACGC 
                   
               
               
                   
                   
                 ATCTGCCGAAGGTTTGGCTAGATTAAACCCTTCCTTCGATAGTTT 
                   
               
               
                   
                   
                 AGGTTCTGTTACAGCTGGTAATGCATCCAGTATTAACGATGGTGC 
                   
               
               
                   
                   
                 CGCTGCAGTCATGATGATGTCAGAAGCTAAAGCAAGAGCCTTGA 
                   
               
               
                   
                   
                 ATTTGCCTGTTTTGGCTAGAATTAGAGCTTTTGCATCCGTTGGTGT 
                   
               
               
                   
                   
                 CGATCCAGCATTGATGGGTATAGCCCCTGTTTATGCTACCAGAAG 
                   
               
               
                   
                   
                 ATGTTTAGAAAGAGTCGGTTGGCAATTGGCTGAAGTAGACTTAA 
                   
               
               
                   
                   
                 TAGAAGCCAACGAAGCTTTCGCCGCTCAAGCATTTGTCTGTTGGTA 
                   
               
               
                   
                   
                 AAATGTTAGAATGGGATGAAAGAAGAGTAAATGTTAACGGTGGT 
                   
               
               
                   
                   
                 GCCATAGCTTTAGGTCATCCAATCGGTGCTAGTGGTTGCAGAATT 
                   
               
               
                   
                   
                 TTGGTTTCTTTAGTCCACGAAATGGTTAAAAGAAATGCTAGAAA 
                   
               
               
                   
                   
                 GGGTTTAGCAACATTGTGTATTGGTGGTGGTCAAGGTGTAGCATT 
                   
               
               
                   
                   
                 GACTATCGAAAGAGACGAATAA 
                   
               
               
                   
               
               
                 55 
                 tdTER 
                 ATGATAGTAAAGCCAATGGTAAGGAACAATATCTGTCTTAACGC 
                 186 
               
               
                   
                   
                 CCATCCACAGGGTTGCAAAAAGGGAGTTGAAGATCAAATTGAAT 
                   
               
               
                   
                   
                 ACACCAAAAAGAGAATTACAGCAGAGGTCAAGGCAGGGGCAAA 
                   
               
               
                   
                   
                 GGCTCCTAAGAACGTCTTAGTTTTGGGTTGTTCTAATGGATACGG 
                   
               
               
                   
                   
                 CTTGGCAAGTAGAATAACTGCAGCCTTCGGTTATGGAGCCGCCA 
                   
               
               
                   
                   
                 CTATAGGTGTATCATTCGAAAAAGCCGGCTCCGAAACCAAGTAC 
                   
               
               
                   
                   
                 GGTACACCTGGCTGGTATAACAATCTAGCTTTTGATGAAGCTGCT 
                   
               
               
                   
                   
                 AAGAGAGAAGGGTTATACTCTGTCACAATAGACGGTGACGCATT 
                   
               
               
                   
                   
                 TTCTGATGAAATCAAAGCTCAGGTTATTGAAGAGGCCAAGAAAA 
                   
               
               
                   
                   
                 AGGGTATCAAATTCGATCTGATAGTATACTCATTAGCATCCCCAG 
                   
               
               
                   
                   
                 TGCGTACAGATCCAGATACTGGCATTATGCACAAATCTGTTTTGA 
                   
               
               
                   
                   
                 AACCATTTGGAAAAACTTTCACTGGTAAAACAGTTGATCCTTTTA 
                   
               
               
                   
                   
                 CAGGAGAACTGAAGGAAATCTCAGCTGAACCAGCTAATGATGAG 
                   
               
               
                   
                   
                 GAGGCAGCTGCTACTGTGAAAGTTATGGGTGGAGAGGACTGGGA 
                   
               
               
                   
                   
                 AAGATGGATCAAACAACTAAGTAAGGAAGGTTTACTTGAAGAGG 
                   
               
               
                   
                   
                 GATGCATCACCTTAGCCTACTCTTACATTGGTCCTGAAGCAACAC 
                   
               
               
                   
                   
                 AAGCCCTATACCGTAAAGGAACTATAGGTAAGGCAAAGGAACAC 
                   
               
               
                   
                   
                 CTTGAAGCTACTGCTCATCGTCTGAATAAGGAAAATCCATCCATT 
                   
               
               
                   
                   
                 AGGGCTTTCGTTAGTGTCAACAAAGGGTTAGTTACCAGAGCATC 
                   
               
               
                   
                   
                 AGCTGTGATCCCTGTCATTCCACTTTACCTTGCTTCATTGTTTAAG 
                   
               
               
                   
                   
                 GTTATGAAAGAGAAAGGCAATCATGAAGGATGTATCGAACAAAT 
                   
               
               
                   
                   
                 CACAAGATTGTACGCTGAGAGATTGTATAGAAAGGATGGTACAA 
                   
               
               
                   
                   
                 TTCCTGTGGACGAAGAGAATAGAATTAGAATCGATGATTGGGAG 
                   
               
               
                   
                   
                 TTAGAAGAGGACGTTCAAAAAGCTGTTTCTGCATTGATGGAAAA 
                   
               
               
                   
                   
                 AGTTACAGGCGAAAATGCTGAGTCACTAACAGACCTGGCAGGTT 
                   
               
               
                   
                   
                 ATAGACATGACTTTTTGGCCTCAAACGGGTTTGATGTAGAAGGTA 
                   
               
               
                   
                   
                 TCAACTACGAAGCTGAAGTCGAAAGATTCGATAGAATCTAA 
                   
               
               
                   
               
               
                 56 
                 tesA 
                 ATGGCCGATACTTTGTTAATTTTGGGTGACTCTTTATCAGCCGGT 
                 187 
               
               
                   
                   
                 TATAGAATGTCCGCTAGTGCTGCATGGCCAGCATTGTTAAACGAT 
                   
               
               
                   
                   
                 AAATGGCAATCTAAGACTTCAGTTGTCAATGCATCTATATCAGGT 
                   
               
               
                   
                   
                 GACACATCACAACAAGGTTTGGCCAGATTACCAGCTTTGTTAAA 
                   
               
               
                   
                   
                 ACAACATCAACCTAGATGGGTCTTGGTAGAATTAGGTGGTAACG 
                   
               
               
                   
                   
                 ATGGTTTGAGAGGTTTTCAACCTCAACAAACCGAACAAACTTTG 
                   
               
               
                   
                   
                 AGACAAATCTTACAAGATGTTAAGGCCGCTAATGCAGAACCATT 
                   
               
               
                   
                   
                 GTTAATGCAAATTAGATTACCTGCCAACTATGGTAGAAGATACA 
                   
               
               
                   
                   
                 ATGAAGCATTTTCTGCAATCTATCCAAAATTGGCAAAGGAATTTG 
                   
               
               
                   
                   
                 ATGTACCATTGTTGCCATTTTTCATGGAAGAAGTTTACTTAAAAC 
                   
               
               
                   
                   
                 CTCAATGGATGCAAGATGACGGTATTCATCCAAACAGAGATGCT 
                   
               
               
                   
                   
                 CAACCTTTTATAGCAGACTGGATGGCCAAACAATTGCAACCATT 
                   
               
               
                   
                   
                 AGTCAATCACGATTCTTGA 
                   
               
               
                   
               
               
                 57 
                 tesB 
                 ATGTCTCAAGCTTTGAAGAACTTGTTGACTTTGTTGAACTTGGAA 
                 188 
               
               
                   
                   
                 AAGATCGAAGAAGGTTTGTTCAGAGGTCAATCTGAAGACTTGGG 
                   
               
               
                   
                   
                 TTTGAGACAAGTTTTCGGTGGTCAAGTTGTTGGTCAAGCTTTGTA 
                   
               
               
                   
                   
                 CGCTGCTAAGGAAACTGTTCCAGAAGAAAGATTGGTTCACTCTTT 
                   
               
               
                   
                   
                 CCACTCTTACTTCTTGAGACCAGGTGACTCTAAGAAGCCAATCAT 
                   
               
               
                   
                   
                 CTACGACGTTGAAACTTTGAGAGACGGTAACTCTTTCTCTGCTAG 
                   
               
               
                   
                   
                 AAGAGTTGCTGCTATCCAAAACGGTAAGCCAATCTTCTACATGA 
                   
               
               
                   
                   
                 CTGCTTCTTTCCAAGCTCCAGAAGCTGGTTTCGAACACCAAAAGA 
                   
               
               
                   
                   
                 CTATGCCATCTGCTCCAGCTCCAGACGGTTTGCCATCTGAAACTC 
                   
               
               
                   
                   
                 AAATCGCTCAATCTTTGGCTCACTTGTTGCCACCAGTTTTGAAGG 
                   
               
               
                   
                   
                 ACAAGTTCATCTGTGACAGACCATTGGAAGTTAGACCAGTTGAA 
                   
               
               
                   
                   
                 TTCCACAACCCATTGAAGGGTCACGTTGCTGAACCACACAGACA 
                   
               
               
                   
                   
                 AGTTTGGATCAGAGCTAACGGTTCTGTTCCAGACGACTTGAGAGT 
                   
               
               
                   
                   
                 TCACCAATACTTGTTGGGTTACGCTTCTGACTTGAACTTCTTGCC 
                   
               
               
                   
                   
                 AGTTGCTTTGCAACCACACGGTATCGGTTTCTTGGAACCAGGTAT 
                   
               
               
                   
                   
                 CCAAATCGCTACTATCGACCACTCTATGTGGTTCCACAGACCATT 
                   
               
               
                   
                   
                 CAACTTGAACGAATGGTTGTTGTACTCTGTTGAATCTACTTCTGC 
                   
               
               
                   
                   
                 TTCTTCTGCTAGAGGTTTCGTTAGAGGTGAATTCTACACTCAAGA 
                   
               
               
                   
                   
                 CGGTGTTTTGGTTGCTTCTACTGTTCAAGAAGGTGTTATGAGAAA 
                   
               
               
                   
                   
                 CCACAACTAA 
                   
               
               
                   
               
               
                 58 
                 fadM 
                 ATGCAAACTCAAATCAAGGTTAGAGGTTACCACTTGGACGTTTA 
                 189 
               
               
                   
                   
                 CCAACACGTTAACAACGCTAGATACTTGGAATTCTTGGAAGAAG 
                   
               
               
                   
                   
                 CTAGATGGGACGGTTTGGAAAACTCTGACTCTTTCCAATGGATGA 
                   
               
               
                   
                   
                 CTGCTCACAACATCGCTTTCGTTGTTGTTAACATCAACATCAACT 
                   
               
               
                   
                   
                 ACAGAAGACCAGCTGTTTTGTCTGACTTGTTGACTATCACTTCTC 
                   
               
               
                   
                   
                 AATTGCAACAATTGAACGGTAAGTCTGGTATCTTGTCTCAAGTTA 
                   
               
               
                   
                   
                 TCACTTTGGAACCAGAAGGTCAAGTTGTTGCTGACGCTTTGATCA 
                   
               
               
                   
                   
                 CTTTCGTTTGTATCGACTTGAAGACTCAAAAGGCTTTGGCTTTGG 
                   
               
               
                   
                   
                 AAGGTGAATTGAGAGAAAAGTTGGAACAAATGGTTAAGTAA 
                   
               
               
                   
               
               
                 59 
                 yciA 
                 ATGTCTACTACTCACAACGTTCCACAAGGTGACTTGGTTTTGAGA 
                 190 
               
               
                   
                   
                 ACTTTGGCTATGCCAGCTGACACTAACGCTAACGGTGACATCTTC 
                   
               
               
                   
                   
                 GGTGGTTTGGTTGATGTCTCAAATGGACATCGGTGGTGCTATCTTG 
                   
               
               
                   
                   
                 GCTAAGGAAATCGCTCACGGTAGAGTTGTTACTGTTAGAGTTGA 
                   
               
               
                   
                   
                 AGGTATGACTTTCTTGAGACCAGTTGCTGTTGGTGACGTTGTTTG 
                   
               
               
                   
                   
                 TTGTTACGCTAGATGTGTTCAAAAGGGTACTACTTCTGTTTCTAT 
                   
               
               
                   
                   
                 CAACATCGAAGTTTGGGTTAAGAAGGTTGCTTCTGAACCAATCG 
                   
               
               
                   
                   
                 GTCAAAGATACAAGGCTACTGAAGCTTTGTTCAAGTACGTTGCTG 
                   
               
               
                   
                   
                 TTGACCCAGAAGGTAAGCCAAGAGCTTTGCCAGTTGAATAA 
                   
               
               
                   
               
               
                 60 
                 ETR1 
                 ATGCTCACTTATGGAGGAATGTCAAAACAACCTGTAACTTTACCA 
                 191 
               
               
                   
                   
                 ACATCTCTACACATTTTCAAAGGCTTGACATCCAAAGGATACTGG 
                   
               
               
                   
                   
                 GTGACTGAAAAGAACAAAAAAAACCCCCAAAGCAAAATTGACA 
                   
               
               
                   
                   
                 CCATCAGTGATTTTATCAAAATGTATAATGATGGTCACATTATTT 
                   
               
               
                   
                   
                 CACCAAGAGATGAAATTGAAACTCTTACCTGGAATACTAACACT 
                   
               
               
                   
                   
                 ACTACTGACGAACAGTTACTAGAACTAGTCAAAAAAGGCATAAC 
                   
               
               
                   
                   
                 TGGGAAGGGGAAGAAAAAAATGGTTGTTTTAGAATGGTAA 
                   
               
               
                   
               
               
                 61 
                 HFA1 
                 ATGAGATCTATAAGAAAATGGGCGTACGAGACGTTCAATG 
                 192 
               
               
                   
                   
                 ATGAAAAAATCATTCAATTCGTGGTAATGGCGACACCTGAT 
                   
               
               
                   
                   
                 GATTTACACGCAAATTCGGAGTATATTAGAATGGCAGACCA 
                   
               
               
                   
                   
                 ATATGTGCAGGTACCAGGGGGTACCAACAACAACAATTAC 
                   
               
               
                   
                   
                 GCCAACATAGACTTAATACTGGACGTGGCAGAGCAAACGG 
                   
               
               
                   
                   
                 ATGTGGATGCGGTCTGGGCTGGATGGGGCCATGCTTCTGAA 
                   
               
               
                   
                   
                 AATCCGTGTCTTCCTGAGCTGTTAGCTAGTTCACAAAGGAA 
                   
               
               
                   
                   
                 AATACTATTCATTGGTCCTCCTGGACGCGCTATGAGATCAT 
                   
               
               
                   
                   
                 TGGGTGACAAGATTTCTTCCACTATTGTAGCACAAAGCGCT 
                   
               
               
                   
                   
                 AAAATCCCGTGTATCCCTTGGTCTGGTTCACATATAGACAC 
                   
               
               
                   
                   
                 TATCCATATCGATAACAAGACGAACTTTGTATCTGTGCCGG 
                   
               
               
                   
                   
                 ATGATGTATATGTAAGGGGATGTTGTTCCTCACCTGAAGAT 
                   
               
               
                   
                   
                 GCTTTAGAAAAGGCTAAATTAATAGGATTTCCTGTAATGAT 
                   
               
               
                   
                   
                 TAAGGCATCCGAAGGTGGTGGAGGTAAGGGCATTAGGCGA 
                   
               
               
                   
                   
                 GTAGATAATGAGGATGATTTTATTGCATTATATCGCCAAGC 
                   
               
               
                   
                   
                 AGTGAATGAGACACCTGGGTCGCCTATGTTTGTTATGAAAG 
                   
               
               
                   
                   
                 TTGTCACTGATGCTCGTCACTTAGAGGTACAGTTATTAGCT 
                   
               
               
                   
                   
                 GACCAATATGGCACTAACATTACATTGTTTGGGAGAGACTG 
                   
               
               
                   
                   
                 TTCCATACAAAGGCGGCACCAAAAGATTATAGAAGAGGCA 
                   
               
               
                   
                   
                 CCAGTGACAATAACCAAGCCTGAAACGTTTCAAAGGATGG 
                   
               
               
                   
                   
                 AACGCGCAGCAATTCGTCTAGGTGAATTGGTAGGTTATGTT 
                   
               
               
                   
                   
                 TCTGCGGGCACTGTCGAATACTTATATTCACCAAAAGATGA 
                   
               
               
                   
                   
                 TAAATTTTACTTTTTAGAACTGAATCCAAGACTACAAGTAG 
                   
               
               
                   
                   
                 AGCATCCAACGACAGAAATGATATCTGGCGTAAACCTTCCT 
                   
               
               
                   
                   
                 GCCACTCAACTGCAAATCGCCATGGGTATTCCTATGCACAT 
                   
               
               
                   
                   
                 GATAAGTGATATCAGAAAACTTTATGGTTTAGATCCAACGG 
                   
               
               
                   
                   
                 GAACTTCGTATATTGATTTTAAAAATTTAAAGAGACCCTCG 
                   
               
               
                   
                   
                 CCAAAAGGCCATTGTATTTCATGCAGGATCACTTCAGAAGA 
                   
               
               
                   
                   
                 TCCTAATGAAGGTTTCAAGCCCTCCACTGGGAAAATACATG 
                   
               
               
                   
                   
                 AGCTCAATTTTCGTTCTTCTTCCAATGTTTGGGGTTACTTCT 
                   
               
               
                   
                   
                 CAGTAGGAAATAATGGTGCTATTCACTCATTTTCAGATTCC 
                   
               
               
                   
                   
                 CAATTTGGGCACATTTTTGCTGTAGGAAACGATAGGCAAGA 
                   
               
               
                   
                   
                 TGCAAAGCAAAACATGGTTTTAGCTCTAAAAGATTTTTCCA 
                   
               
               
                   
                   
                 TCCGAGGAGAATTCAAAACCCCTATAGAGTACCTGATAGA 
                   
               
               
                   
                   
                 GCTATTAGAAACTCGGGACTTTGAGAGTAATAACATATCGA 
                   
               
               
                   
                   
                 CTGGTTGGTTAGATGATTTGAMTGAAAAATTTATCTTCCG 
                   
               
               
                   
                   
                 ATAGCAAACTAGATCCAACGCTCGCTATTATCTGTGGTGCC 
                   
               
               
                   
                   
                 GCAATGAAAGCATACGTTTTCACAGAAAAGGTGAGGAATA 
                   
               
               
                   
                   
                 AGTATTTGGAATTATTGCGGAGGGGCCAAGTTCCACCTAAA 
                   
               
               
                   
                   
                 GATTTTCTTAAAACGAAGTTTCCTGTTGACTTCATTTTCGAT 
                   
               
               
                   
                   
                 AATAATAGATACTTGTTCAATGTTGCTCAATCATCTGAAGA 
                   
               
               
                   
                   
                 ACAATTTATTCTTTCTATCAATAAGTCTCAATGTGAAGTTAA 
                   
               
               
                   
                   
                 TGTTCAAAAATTGTCCGGTGACTGCTTGTTGATCTCCGTTGA 
                   
               
               
                   
                   
                 CGGTAAATGCCATACAGTTTATTGGAAGGACGATATCAGA 
                   
               
               
                   
                   
                 GGTACAAGACTTTCGATAGACTCCAATACCATATTTTTAGA 
                   
               
               
                   
                   
                 AGCTGAACTCAATCCCACTCAAGTGATCTCTCCAACTCCGG 
                   
               
               
                   
                   
                 GGAAATTGGTGAAATATTTGGTCCGAAGTGGTGATCACGTT 
                   
               
               
                   
                   
                 TTTGCTGGACAGCAATATGCAGAAATAGAAATAATGAAAA 
                   
               
               
                   
                   
                 TGCAGATGCCACTAGTAGCGAAAAGTGATGGTGTAATTGA 
                   
               
               
                   
                   
                 GTTACTAAGACAGCCCGGTTCCATAATTGAGGCTGGTGATG 
                   
               
               
                   
                   
                 TCATCGCAAAATTGACTTTGGATTCACCGTCCAAAGCTAAC 
                   
               
               
                   
                   
                 GAATCGTCTTTATACCGCGGAGAATTACCTGTTTTAGGTCC 
                   
               
               
                   
                   
                 ACCGCTAATAGAGGGTAGCCGACCAAACCATAAGCTCAGA 
                   
               
               
                   
                   
                 GTCTTAATAAATAGGTTAGAAAATATTCTCAATGGATATCA 
                   
               
               
                   
                   
                 TGAAAACTCTGGAATAGAAACTACTCTAAAAGAGTTGATA 
                   
               
               
                   
                   
                 AAAATATTGAGAGATGGTAGGCTTCCTTATTCAGAATGGGA 
                   
               
               
                   
                   
                 TTCCCAAATTTCTACGGTACGCAATAGACTACCAAGGCAAT 
                   
               
               
                   
                   
                 TGAATGAGGGGCTGGGAAATCTAGTCAAGAAATCTGTTTCT 
                   
               
               
                   
                   
                 TTTCCTGCAAAGGAACTGCACAAATTAATGAAGCGCTACTT 
                   
               
               
                   
                   
                 GGAAGAAAATACAAATGATCATGTAGTTTATGTTGCCTTAC 
                   
               
               
                   
                   
                 AGCCACTTCTTAAAATTAGTGAAAGGTATAGCGAAGGTTTA 
                   
               
               
                   
                   
                 GCTAATCACGAATGTGAAATTTTTTTAAAGTTGATTAAAAA 
                   
               
               
                   
                   
                 GTATTATGCTGTTGAGAAAATTTTTGAAAATCATGATATAC 
                   
               
               
                   
                   
                 ATGAAGAAAGAAACTTACTAAATCTGCGGAGGAAAGACCT 
                   
               
               
                   
                   
                 TACAAACTTAAAAGAAATTTTGTGCATAAGTTTATCGCATG 
                   
               
               
                   
                   
                 CTAACGTAGTCGCAAAGAACAAGTTAGTAACTGCAATATTG 
                   
               
               
                   
                   
                 CATGAATACGAGCCATTGTGCCAGGATTCCTCTAAGATGTC 
                   
               
               
                   
                   
                 TTTAAAATTCAGGGCTGTTATACATGATTTGGCAAGTTTGG 
                   
               
               
                   
                   
                 AATCTAAGTGGGCTAAGGAGGTTGCTGTAAAGGCAAGATC 
                   
               
               
                   
                   
                 AGTGCTACTCAGAGGGATTTTCCCTCCCATAAAGAAAAGAA 
                   
               
               
                   
                   
                 AAGAGCATATTAAAACTCTCCTGCAATTGCACATAAAGGAT 
                   
               
               
                   
                   
                 ACTGGTGCCAAAAACATTCACAGCAGGAACATATATTCCTG 
                   
               
               
                   
                   
                 TATGAGGGATTTTGGTAATTTAATACATTCAAATCTGATAC 
                   
               
               
                   
                   
                 AACTTCAGGATTTGTTCTTTTTTTTTGGCCATCAAGATACGG 
                   
               
               
                   
                   
                 CTCTTTCCAGTATAGCATCTGAAATTTATGCAAGGTATGCC 
                   
               
               
                   
                   
                 TACGGCAATTATCAATTAAAAAGTATTAAGATTCACAAAGG 
                   
               
               
                   
                   
                 AGCGCCTGATTTACTAATGTCATGGCAATTCAGCTCATTAA 
                   
               
               
                   
                   
                 GAAATTATTTAGTCAATCCTGATGGTGAGAGTGATGAGTTT 
                   
               
               
                   
                   
                 ACAAAACTTTCTAAACCTCCCTCAACATCAGGTAAGAGCTC 
                   
               
               
                   
                   
                 AGCAAATAGTTTTGGTCTTCTTGTCAACATGCGTGCGCTTG 
                   
               
               
                   
                   
                 AATCTCTGGAAAAGACATTAGACGAGGTATACGAACAAAT 
                   
               
               
                   
                   
                 TCATATTCCTGAGGAAAGACTTTCCAGCGGAGAGAACTCTC 
                   
               
               
                   
                   
                 TTATTGTTAATATTTTATCTCCTATTCGTTACAGAAGTGAAA 
                   
               
               
                   
                   
                 ATGATCTAATTAAAACTTTAAAAATTAAACTTCATGAAAAT 
                   
               
               
                   
                   
                 GAGAGAGGTCTATCCAAGCTCAAGGTTAATCGTATTACATT 
                   
               
               
                   
                   
                 TGCATTTATCGCCGCGAATGCGCCCACTGTTAAATTTTACTC 
                   
               
               
                   
                   
                 CTTTGATGGAACTACGTACGATGAAATCTCTCAAATAAGAA 
                   
               
               
                   
                   
                 ATATGGATCCATCCTATGAAGCACCGTTAGAGTTAGGAAAA 
                   
               
               
                   
                   
                 ATGTCGAACTATAAAATCAGATCACTACCTACATACGATAG 
                   
               
               
                   
                   
                 TAGTATACGCATTTTTGAAGGTATTAGCAAATTTACGCCGC 
                   
               
               
                   
                   
                 TAGATAAAAGGTTCTTTGTCAGGAAAATCATAAATTCCTTC 
                   
               
               
                   
                   
                 ATGTATAATGATCAAAAAACAACCGAAGAAAACTTGAAAG 
                   
               
               
                   
                   
                 CGGAAATCAATGCTCAAGTGGTTTATATGTTAGAACATCTA 
                   
               
               
                   
                   
                 GGAGCAGTTGACACCTCAAATTCAGACTTGAATCATATTTT 
                   
               
               
                   
                   
                 TTTAAGTTTCAATACAGTTCTTAACATACCAGTACATCGTCT 
                   
               
               
                   
                   
                 CGAGGAAATTGTGAGTACAATTCTAAAGACTCACGAAACC 
                   
               
               
                   
                   
                 AGATTGTTTCAAGAAAGAATCACAGATGTAGAAATTTGCAT 
                   
               
               
                   
                   
                 CTCTGTTGAGTGCCTAGAAACAAAGAAGCCAGCCCCGCTTA 
                   
               
               
                   
                   
                 GATTACTTAT&#39;FTCTAATAAATCTGGGTATGTGGTAAAAATT 
                   
               
               
                   
                   
                 GAGACATATTACGAAAAGATAGGGAAAAATGGGAATCTGA 
                   
               
               
                   
                   
                 TTTTGGAACCGTGTAGTGAGCAGAGCCATTATAGCCAGAAA 
                   
               
               
                   
                   
                 TCTCTCTCTCTTCCTTACTCGGTCAAGGATTGGCTACAACCT 
                   
               
               
                   
                   
                 AAAAGATACAAAGCTCAATTCATGGGTACAACATATGTGT 
                   
               
               
                   
                   
                 ACGATTTCCCAGGTCTGTTTCATCAAGCTGCAATCCAACAG 
                   
               
               
                   
                   
                 TGGAAAAGGTATTTTCCAAAACATAAGCTGAATGACAGTTT 
                   
               
               
                   
                   
                 TTTTAGTTGGGTTGAATTGATAGAACAAAACGGTAATTTGA 
                   
               
               
                   
                   
                 TAAAAGTAAACAGGGAGCCAGGCCTTAATAATATAGGGAT 
                   
               
               
                   
                   
                 GGTT 
                   
               
               
                   
                   
                 GCTTTTGAGATTATGGTTCAGACACCTGAATATCCTGAAGG 
                   
               
               
                   
                   
                 GCGTAACATGATCGTGATTTCTAATGATATTACCTACAATA 
                   
               
               
                   
                   
                 TTGGATCTTTTGGACCGAGAGAAGATTTGTTTTTTGATAGG 
                   
               
               
                   
                   
                 GTCACAAATTATGCAAGAGAGAGAGGGATCCCGAGGATAT 
                   
               
               
                   
                   
                 ACTTGGCGGCGAATTCAGGAGCTAAATTGGGTATAGCCGA 
                   
               
               
                   
                   
                 AGAGCTGATCCCTCTATTTCGTGTAGCATGGAATGACCCCT 
                   
               
               
                   
                   
                 CTGATCCAACAAAGGGTTTCCAGTACTTATACTTAGCTCCA 
                   
               
               
                   
                   
                 AAAGACATGCAGCTACTGAAAGATTCTGGGAAAGGAAATT 
                   
               
               
                   
                   
                 CGGTTGTTGTTGAACACAAGATGGTATACGGTGAAGAGAG 
                   
               
               
                   
                   
                 ATATATTATTAAAGCAATAGTCGGATTCGAAGAGGGTTTAG 
                   
               
               
                   
                   
                 GTGTTGAATGTTTACAGGGCTCAGGTTTAATTGCTGGTGCC 
                   
               
               
                   
                   
                 ACTTCGAAAGCGTATAGAGACATTTTCACTATTACTGCTGT 
                   
               
               
                   
                   
                 TACTTGTCGGTCCGTTGGTATAGGTTCCTATCTGGTCAGACT 
                   
               
               
                   
                   
                 AGGACAACGTACTATTCAGGTGGAGGATAAGCCTATCATA 
                   
               
               
                   
                   
                 CTGACGGGTGCATCGGCGATTAATAAAGTTTTGGGTACCGA 
                   
               
               
                   
                   
                 TATCTATACATCTAACCTACAAATTGGCGGAACCCAAATCA 
                   
               
               
                   
                   
                 TGTATAAAAACGGAATAGCGCATTTAACAGCCAGTAATGA 
                   
               
               
                   
                   
                 TATGAAAGCCATCGAAAAAATAATGACATGGTTATCATATG 
                   
               
               
                   
                   
                 TCCCGGCGAAAAGAGATATGAGTCCTCCACTTCTTGAAACT 
                   
               
               
                   
                   
                 ATGGATAGATGGGATAGGGATGTAGACTTCAAACCTGCCA 
                   
               
               
                   
                   
                 AGCAAGTGCCATATGAGGCAAGGTGGTTGATAGAGGGTAA 
                   
               
               
                   
                   
                 ATGGGACTCAAATAACAACTTCCAGTCAGGCCTATTTGATA 
                   
               
               
                   
                   
                 AGGATTCGTTTTTTGAGACATTATCTGGATGGGCCAAAGGT 
                   
               
               
                   
                   
                 GTAATAGTTGGAAGAGCACGTCTTGGAGGTATTCCCGTAGG 
                   
               
               
                   
                   
                 TGTTATTGCGGTAGAAACTAAGACTATCGAAGAAACAATCC 
                   
               
               
                   
                   
                 CCGCTGACCCAGCTAATCTGGATTCTTCAGAGTTTTCCGTTA 
                   
               
               
                   
                   
                 AAGAAGCAGGACAGGTGTGGTATCCAAATTCCGCGTTCAA 
                   
               
               
                   
                   
                 AACAGCTCAAACTATAAATGATTTTAACTATGGTGAGCAAT 
                   
               
               
                   
                   
                 TACCATTGATTATCTTAGCCAATTGGAGGGGATTTTCTGGC 
                   
               
               
                   
                   
                 GGTCAAAGGGATATGTACAATGAAGTACTAAAGTACGGGT 
                   
               
               
                   
                   
                 CTTTTATTGTTGACGCTCTGGTTGACTACAAACAACCCATA 
                   
               
               
                   
                   
                 CTGATATACATTCCGCCCTTTGGTGAATTAAGGGGCGGATC 
                   
               
               
                   
                   
                 ATGGGTTGTTATAGATCCAACTATTAATCCTGAACAAATGG 
                   
               
               
                   
                   
                 AAATGTATGCCGATGTTGAATCTAGGGGAGGTGTGTTAGAA 
                   
               
               
                   
                   
                 CCTGACGGAGTAGTTAGCATAAAATACCGTAAGGAGAAAA 
                   
               
               
                   
                   
                 TGATAGAGACGATGATTCGATTAGACTCCACATATGGACAT 
                   
               
               
                   
                   
                 TTGAGAAGAACGTTGACAGAAAAAAAGTTATCTTTGGAAA 
                   
               
               
                   
                   
                 AACAAAATGATCTTACGAAGAGATTGAAAATAAGAGAGAG 
                   
               
               
                   
                   
                 ACAGTTGATACCAATTTATAATCAAATCAGCATACAGTTTG 
                   
               
               
                   
                   
                 CAGATTTACATGATAGATCGACTAGGATGCTAGTTAAAGGA 
                   
               
               
                   
                   
                 GTAATCCGAAAGGAGTTGGAATGGAAAAAGTCACGCAGAT 
                   
               
               
                   
                   
                 TTTTATATTGGAGACTGAGAAGGAGGTTGAACGAGGGACA 
                   
               
               
                   
                   
                 AGTGATCAAAAGACTGCAAAAAAAAACATGTGATAACAAA 
                   
               
               
                   
                   
                 ACGAAAATGAAATACGACGACCTGTTGAAAATAGTTCAGT 
                   
               
               
                   
                   
                 CATGGTATAACGATCTGGATGTTAATGATGACAGAGCAGTA 
                   
               
               
                   
                   
                 GTGGAGTTCATAGAAAGAAATTCGAAAAAAATTGACAAGA 
                   
               
               
                   
                   
                 ACATTGAAGAGTTTGAGATCTCGCTGTTGATCGATGAGCTT 
                   
               
               
                   
                   
                 AAGAAAAAATTTGAAGACAGAAGGGGAAACATTGTCCTTG 
                   
               
               
                   
                   
                 AAGAGCTAACTAGGTTGGTGGACAGTAAGCGAAAGAGATA 
                   
               
               
                   
                   
                 G 
                   
               
               
                   
               
               
                 62 
                 3xARE1 + 
                 AATAAGGATCTCGAACCTTGTGCGATGACAACAGCATGTG 
                 193 
               
               
                   
                 pTEF1core 
                 AATAAGGATCTCGAACCTTGTGCGATGACAACAGCATGTG 
                   
               
               
                   
                   
                 AATAAGGATCTCGAACCTTGTGCGATGACAACAGCATGTG 
                   
               
               
                   
                   
                 AATAAGGATCTCGAACCATTGATATTTAAGTTAATAAACGG 
                   
               
               
                   
                   
                 TCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATT 
                   
               
               
                   
                   
                 ACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAG 
                   
               
               
                   
                   
                 CAATCTAATCTAAGTTTTAATTACAAA 
                   
               
               
                 63 
                 pTEF1 
                 CACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTC 
                 194 
               
               
                   
                 (3xARE1) 
                 TTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCA 
                   
               
               
                   
                   
                 AAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTC 
                   
               
               
                   
                   
                 CTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAA 
                   
               
               
                   
                   
                 AGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAA 
                   
               
               
                   
                   
                 AGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATT 
                   
               
               
                   
                   
                 TTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATA 
                   
               
               
                   
                   
                 TTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTT 
                   
               
               
                   
                   
                 CATTTTTCTTGTTCCTTGTGCGATGACAACAGCATGTGTATT 
                   
               
               
                   
                   
                 ACAACTTTTTTTACTTCTTCTTGTGCGATGACAACAGCATGT 
                   
               
               
                   
                   
                 GGCTCATTAGAAACTTGTGCGATGACAACAGCATGTGGAA 
                   
               
               
                   
                   
                 AGCATAGCAATCTAATCTAAGTTTTAATTACAAA