Abstract:
The present invention concerns a pipette comprising a mechanism with which the stroke length of a piston can be changed by rotating the piston rod. The pipette comprises a locking mechanism which prevents the rotation of the piston rod when the piston has been pushed a certain distance downwards from its upper position, but does not prevent the rotation when the piston is in its upper position.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
       [0001]    This application is a submission under 35 U.S.C. §371 of International Application No. PCT/FI2012/051003, filed Oct. 18, 2012, which claims priority to Finnish Application No. 20116037, filed Oct. 20, 2011, the disclosures of which are hereby expressly incorporated by reference herein in their entireties. 
     
    
     FIELD OF THE INVENTION 
       [0002]    The present invention relates to liquid dispensing and concerns a pipette, in which the dosage volume is set by rotating the piston rod and which comprises a locking device with which accidental change of set volume is prevented. 
       BACKGROUND OF THE INVENTION 
       [0003]    Piston pipettes, which comprise a piston rod extending above the handle, are used for liquid dispensing, for example, in laboratories. The piston can thus be moved by the rod. Also, such pipettes are used in which the length of the intake movement of the piston can be set by rotating the rod. Such a pipette comprises also a volume display, typically based on digit rings, on which the set volume is displayed. One problem of these kinds of pipettes is that the rod may be accidentally rotated during pipetting, whereby also the set volume accidentally changes. In document WO 84/00119, a pipette is described in which for preventing accidental rotation there is a ring-like spring above the uppermost digit ring, the spring comprising at its lower surface protrusions and, corresponding to these, hollows on the upper surface of the ring. The rod can be rotated stepwise against the force of the spring. 
         [0004]    The pipette according to document U.S. Pat. No. 4,909,991 comprises around the piston rod a flange non-rotatable with the rod. The body comprises above the flange a threaded sleeve, the upper end of which extends above the body. By rotating the sleeve, its lower end can be pressed tightly against the flange, whereby friction force prevents the accidental rotation of the rod. 
         [0005]    The pipette according to document WO 91/16976 comprises a sleeve rotating with the piston rod and having longitudinal grooves on its outer surface. In the body there is a locking device comprising a spring and at its end a claw settling against a groove. The rod can be stepwise rotated against the force of the spring. The locking device can be, however, lifted up above the grooves, whereby the rod is freely rotatable. For this the locking device comprises an operation peg extending outside the body. 
       SUMMARY OF THE INVENTION 
       [0006]    A pipette according to the present invention comprises a locking mechanism which prevents the rotation of the piston rod when the piston has been pushed a certain distance downwards from its upper position, but does not prevent the rotation when the piston is in its upper position. Thus the change of volume setting is prevented during liquid dispensing. In this way no additional operations are needed in order to prevent a setting change, but the mechanism functions automatically when the piston is pushed downwards. A sufficient movement distance for starting the mechanism in action may be, e.g., 0.5-3 mm, preferably 1-2 mm. 
         [0007]    The locking mechanism may comprise a locking pin which presses against a part rotating with the rod. This rotating part may be, e.g., a part of a digit ring display, such as the step spring of a digit ring which stops the digit rings so that the digits of the rings match to each other. 
         [0008]    The locking mechanism may comprise a locking spring which, when in locking position, presses a locking pin. 
         [0009]    The mechanism may prevent the rotation with the help of friction. However, it is also possible to arrange a mechanism in which the rotation is prevented by form locking. 
     
    
     
       DESCRIPTION OF THE DRAWINGS 
         [0010]    The enclosed drawings are a part of the written description of the present invention, and they relate to the detailed description of some embodiments of the present invention presented next. In the drawings, 
           [0011]      FIG. 1  shows a pipette in accordance with the present invention from front, 
           [0012]      FIG. 2  shows the upper part of the pipette from behind as an enlarged cut figure, 
           [0013]      FIG. 3  shows the partial enlargement A of  FIG. 2 , 
           [0014]      FIG. 4  shows the locking sleeve of the pipette obliquely from above, and 
           [0015]      FIG. 5  the locking sleeve obliquely from above in another angle. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0016]    A pipette in accordance with the figures comprises at its lower end a tip part  1 , at the lower end of which there is a pipette tip or tip  2  for liquid. On the tip part there is a sliding tip removal sleeve, and on the side of body there is a tip remover push button  3  as an extension of the sleeve, which button has been forced to move along the body by means of a wheel in order to decrease the operation force (see, e.g., U.S. Pat. No. 5,435,197). In the upper end of the body on the opposite side from the button there is a finger support. In the body there is a cylinder and as an extension thereof an aspirating channel going through the tip part. In the cylinder there is a moveable piston  5  having an extending piston rod  6 , in the upper end of which there is a piston knob  7  above the upper end of the body. The piston is moved by pushing the knob. The desired dispensing volume is set by rotating the rod by the knob. The set volume is displayed in the opening on the side of the body by a digit ring display  8  having four digit rings. 
         [0017]    In the tip part there is arranged around the piston  5  a primary spring (not in the drawings), which presses the piston against the piston rod  6 . The piston rod comprises at its lower end a hollow, in which the upper end of the piston settles. The piston rod comprises an adjustment thread  9 , on which an adjustment nut  10  has been fitted so that its rotation in relation to the body has been prevented. In the body there is a fixed limiter  11 . A sliding locking sleeve  12  has been fitted around the piston rod. The primary spring presses the adjustment nut against a flange at the upper end of the locking sleeve, whereby the adjustment nut and the locking sleeve settle against the fixed limiter. The adjustment nut and the fixed limiter thus define the upper limit of the piston stroke. Around the upper part of the rod there is placed a limiter piece  13  having at its upper end a broader limiter flange. Around the limiter piece there is fitted a sliding sleeve-like secondary support  14  having a flange on its outer surface. Above the secondary support there is fitted in a boring in the handle a calibrating screw  15 . Between the fixed limiter and the flange in the secondary support there is fitted a secondary spring  16 , which is stronger than the primary spring, the secondary spring thus pressing the secondary support against the calibration screw. 
         [0018]    When the piston rod is pushed, it moves downwards against the force of the primary spring until the flange of the limiter piece  13  meets the upper end of the secondary support  14 . When the pipette tip  2  is now brought into the liquid to be aspirated and the piston is allowed to return to its upper position, a liquid volume corresponding to the stroke length is received into the tip. By rotating the calibration screw  15 , the lower limit of the aspiration movement may be slightly changed and thus the volume calibrated precisely to correspond to the nominal volume. When removing the liquid, after the limit piece meets the secondary support, the rod is pushed still more, whereby also the secondary spring  16  yields in and the piston moves further lower. Thus the liquid is removed as completely as possible. 
         [0019]    The volume display  8  is based on digit rings. On the piston rod  6  there is fitted sliding, but non-rotating, the uppermost digit ring  17 . It rotates by means of the first step wheel the second digit ring, which rotates the third, and the fourth. The uppermost digit ring comprises a projection, on the upper surface of which a stepping spring  18  is attached. It stops the digit ring on its place so that the digit set on the ring is seen in the middle of the body opening. With a small force the digit ring can be rotated, however. A snapping sound is formed from the step movement. Corresponding volume adjustments have been described, e.g., in documents EP 112 887, and WO 2005/050554. 
         [0020]    On the outer surface of the locking sleeve  12 , there is a longitudinal groove, on the lower end of which there is attached a locking pin  19 . A locking spring  20  presses the locking pin downwards in the groove. The upper end of the locking spring is against the fixed limiter  11 . When the piston is pushed downwards, the locking spring pushes the locking sleeve downwards, whereby the locking pin presses against the stepping spring  18  preventing the uppermost digit ring from rotating. Thus the volume setting is not accidentally changed when the liquid is dispensed. The move distance of the locking pin may be, e.g., 1-2 mm. Using a sufficiently great force, the knob can, however, be rotated also in the locking position. Thus, for instance, accidental breakage of the construction may be prevented. Thereby, the locking force may also be changed by replacing the locking spring with a stronger or weaker one. The locking may be made also completely non-sliding by arranging for the locking pin locking holes, into which the lower end of the pin protrudes. 
         [0021]    While the present invention has been illustrated by description of various embodiments and while those embodiments have been described in considerable detail, it is not the intention of Applicants to restrict or in any way limit the scope of the appended claims to such details. Additional advantages and modifications will readily appear to those skilled in the art. The present invention in its broader aspects is therefore not limited to the specific details and illustrative examples shown and described. Accordingly, departures may be made from such details without departing from the spirit or scope of Applicant&#39;s invention.