Abstract:
The present invention relates to use of a composition in the manufacture of a health care product or medicament for preventing and treating leucopenia induced by radiotherapy or chemotherapy. The composition is made from raw materials comprising 5 to 200 parts by weight of Ganoderma, 5 to 150 parts by weight of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, 1 to 90 parts by weight of fermented  Cordyceps sinensis  powder and/or 1 to 120 parts by weight of Cordyceps, or is a composition consisting of water and/or alcohol extracts of the above raw materials as active components.

Description:
TECHNICAL FIELD 
       [0001]    The present invention relates to a composition for prevention and treatment of leucopenia induced by radiotherapy or chemotherapy and use of the composition. 
       BACKGROUND ART 
       [0002]    Leucopenia refers to a condition where the absolute count of peripheral blood leucocytes is constantly less than 4.0×10 9 /L. Cytotoxic drugs, toxic chemicals, and ionizing radiation, being the most common causes of decrease in neutrophils, may directly act on the stem cell pool and mitotic pool, impairing, damaging or suppressing hematopoietic stem cells/progenitor cells as well as early dividing cells. Some drugs may interfere with protein synthesis or cell division in a dose-dependant manner, while other drugs have dose-independent actions, possibly induced by allergic or immunological factors. Generally, patients with a slight decrease may mostly show primary symptoms, without clinically specific symptoms. Patients with a moderate or severe decrease are susceptible to infections and non-specific symptoms such as fatigue, weakness, dizziness, loss of appetite, etc. Leucopenia induced by radiotherapy or chemotherapy is the main reason why patients with malignant tumors often fail to complete the therapy. Administration of leukogenic drugs during an early stage of leucocyte decrease after radiotherapy or chemotherapy may substantially increase the number of leucocytes and allow patients to effectively and smoothly complete the chemotherapy and to improve their survival and quality of life, which represents a new approach to prevention and treatment of tumors. Patent Application CN101292742A discloses a pharmaceutical composition comprising Ganoderma, Radix Panacis Quinquefolii and fermented  Cordyceps sinensis  powder, which functions to enhance immunity. Patent Application CN102228252A discloses a composition of traditional Chinese medicines comprising Radix Panacis Quinquefolii and/or Radix Et Rhizoma Ginseng, Ganoderma, and fermented  Cordyceps sinensis  powder, which functions to alleviate physical fatigue. Patent Application CN102000129A discloses a pharmaceutical composition comprising Cordyceps polysaccharides or fermented  Cordyceps sinensis  powder, Ganoderma, and Radix Panacis Quinquefolii, which functions to enhance immunity. Up till now, no report or literature shows that a composition made from Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps has a function of preventing and treating leucopenia induced by radiotherapy or chemotherapy. 
       SUMMARY OF THE INVENTION 
       [0003]    The objective of the present invention is to provide a composition for prevention and treatment of leucopenia induced by radiotherapy or chemotherapy and use thereof. 
         [0004]    In accordance with the present invention, we select and combine raw materials Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, and we surprisingly found that such a composition was capable of preventing and treating leucopenia induced by radiotherapy or chemotherapy. 
         [0005]    The present invention provides a composition for prevention and treatment of leucopenia induced by radiotherapy or chemotherapy, which is made from raw materials including the following substances in parts by weight: 5 to 200 parts of Ganoderma, 5 to 150 parts of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, 1 to 90 parts of fermented  Cordyceps sinensis  powder and/or 1 to 120 parts of Cordyceps. The composition comprises the raw materials in parts by weight as above or consists of water and/or alcohol extracts of these raw materials as active components. Alternatively, the composition is obtained by water and/or alcohol extraction of a mixture of these raw materials, or consists of extracts as active components obtained by water and/or alcohol extraction of one or more of these raw materials. 
         [0006]    Preferred are 20 to 120 parts of Ganoderma, 10 to 90 parts of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, 3 to 60 parts of fermented  Cordyceps sinensis  powder and/or 3 to 90 parts of Cordyceps. 
         [0007]    More preferred are 40 parts of Ganoderma, 30 parts of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, 20 parts of fermented  Cordyceps sinensis  powder and/or 6.7 parts of Cordyceps. 
         [0008]    The composition of the present invention may further comprise the following additional materials that do not compromise the efficacy of the present invention, or water and/or alcohol extracts of these additional materials, in parts by weight: one or more of 5 to 90 parts of Flos Rosae Rugosae, 5 to 150 parts of Ganoderma spore powder, 1 to 90 parts of Ganoderma spore oil, 10 to 400 parts of Radix Pseudostellariae, 1 to 120 parts of Folium Ginseng, 3 to 400 parts of Radix Codonopsis and 3 to 400 parts of Radix Astragali, or any combination thereof. 
         [0009]    Preferred are one or more of 10 to 60 parts of Flos Rosae Rugosae, 10 to 120 parts of Ganoderma spore powder, 10 to 60 parts of Ganoderma spore oil, 20 to 200 parts of Radix Pseudostellariae, 20 to 90 parts of Folium Ginseng, 20 to 200 parts of Radix Codonopsis and 20 to 200 parts of Radix Astragali, or any combination thereof. 
         [0010]    More preferred are one or more of 30 parts of Flos Rosae Rugosae, 30 parts of Ganoderma spore powder, 20 parts of Ganoderma spore oil, 40 parts of Radix Pseudostellariae, 30 parts of Folium Ginseng, 40 parts of Radix Codonopsis and 40 parts of Radix Astragali, or any combination thereof. 
         [0011]    Most preferably, the composition of the present invention is a composition of 5 to 200 parts of Ganoderma, 5 to 150 parts of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, 1 to 90 parts of fermented  Cordyceps sinensis  powder and/or 1 to 120 parts of Cordyceps, and 5 to 90 parts of Flos Rosae Rugosae. 
         [0012]    Preferably, the composition of the present invention is made from 20 to 120 parts of Ganoderma, 10 to 90 parts of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, 3 to 60 parts of fermented  Cordyceps sinensis  powder and/or 3 to 90 parts of Cordyceps, and 10 to 60 parts of Flos Rosae Rugosae. 
         [0013]    Most preferably, the composition of the present invention is made from 40 parts of Ganoderma, 30 parts of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, 20 parts of fermented  Cordyceps sinensis  powder and/or 6.7 parts of Cordyceps, and 30 parts of Flos Rosae Rugosae. 
         [0014]    In accordance with the present invention, Folium Ginseng, Radix Pseudostellariae, Radix Codonopsis, or Radix Astragali may be used instead of Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, used in an amount the same as the amount in which they are additionally comprised in the composition of the present invention. 
         [0015]    The term “Ganoderma”, as used herein, refers to the dry sporocarp of fungal species  Ganoderma lucidum  (Leyss. ex Fr.) Karst. or  Ganoderma sinense  Zhao, Xu et Zhang of the family Polyporaceae. It has a sweet taste and a plain nature, is involved in the heart, lung, liver and kidney channels, and has the effects of nourishing physical strength and calming and tranquilizing the mind. The term “Radix Et Rhizoma Ginseng”, as used herein, refers to the dry root and rootstock of the plant species  Panax ginseng  C. A. Mey. of the family Araliaceae. It may be various types of ginseng, such as garden ginseng, wild ginseng, dried fresh ginseng, dried fresh wild ginseng, sugar-processed ginseng, and red ginseng. The term Folium Ginseng refers to the dry leaves of the plant species  Panax ginseng  C. A. Mey. of the family Araliaceae. The term “Radix Panacis Quinquefolii”, as used herein, also known as American ginseng,  huaqishen, yangshen , or  guangdongshen , refers to the dry root of the plant species  Panax quinquefolium  L. of the family Araliaceae. It has a sweet and slightly bitter taste and a cool nature, is involved in the heart, lung and kidney channels, and has the effects of invigorating Qi, nourishing Yin, clearing heat, and promoting fluid production. The term “Cordyceps”, as used herein, refers to a dry complex from a dead body of an insect larva of the family Hepialidae and a stroma of the fungal species  Cordyceps sinensis  (Berk.) sace. of the family Clavicipitaceae parasitizing on the larva. 
         [0016]    The term “fermented  Cordyceps sinensis  powder”, as used herein, refers to a product of strains that were originally isolated from the natural Cordyceps of  Cordyceps sinensis  (Berk.) sace. and have been cultured under fermentation conditions, wherein the strains may be one of  Paecilomyces hepialli  Chen et Dai, sp.nov,  Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov,  Cephalosporium sinensis  Chen sp.nov,  Mortiscrslla hepialid  C. T.&amp;B. liu,  Paecilomyces sinensis  Chen, Xiao et Shi, sp.nov,  Tolypocladium sinensis  C. lan Li,  Cephalosporium sinens  Chen sp.nov,  Scytalidium hepialii  C. L. Li,  Chrysosporium sinens  Z. Q. liang,  Verticillium sinens  Wamg sp.nov,  Cephalosporium acremonium  Corda, Icones Fungorum,  Synnematium sinensis  Yin &amp; Shen,  Isaria farinose  (Holmsk.) Fr. Systema Mycologicum,  Metarhizium anisopliae  (Metsch) Sorokin,  Hirsutella hepialid  Chen et Shen,  Sporothrix insectorum  de Hong &amp; H. C. Evans,  Gliocladium roseum  (link) Thom, and  Mortierella  sp., or any combination thereof. 
         [0017]    The strain from which the fermented  Cordyceps sinensis  powder of the present invention is derived is preferably one of  Paecilomyces  hepialli Chen et Dai, sp.nov,  Mortiscrslla hepialid  C. T.&amp;B. liu,  Synnematium sinensis  Yin &amp; Shen,  Gliocladium roseum  (link) Thom,  Mortierella  sp.,  Cephalosporium sinensis  Chen sp.nov or  Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov, or any combination thereof. 
         [0018]    The term “Flos Rosae Rugosae”, as used herein, refers to the dry flower bud of the plant species  Rosa rugosa  Thumb or  Rose rugosacv  Plena of the family Rosaceae. It has a pungent, sweet and slightly bitter taste and a warm nature, and represents a warm-natured drug. Its most significant effects are to activate Qi flowing, resolve stagnation, harmonize the blood, and relieve pain. 
         [0019]    The term “Radix Codonopsis” refers to the dry root of the plant species  Codonopsis pilosula  (Franch.) Nannf.,  Codonopsis pilosula  Nannf.var.modesta (Nannf.) L. T. Shen, or  Codonopsis tangshen  Oliv. of the family Campanulaceae. 
         [0020]    The term “Radix Pseudostellariae” used here in refers to the dry tuberous root of the plant species  Pseudostellaria heterophylla  (Miq.) Pax ex Pax et Hoffm. of the family Cargophyllaceae. 
         [0021]    The term “Folium Ginseng” refers to the dry leaves of the plant species  Panax ginseng  C. A. Mey. of the family Araliaceae. 
         [0022]    The term “Radix Astragali” refers to the dry root of the plant species  Astragalus membranaceus  (Fisch) Bge.var.mongholicus (Bge) Hsiao or  Astragalus membranaceus  (Fisch) Bge. of the family Fabaceae. 
         [0023]    The Ganoderma spore powder according to the present invention is preferably sporoderm-broken Ganoderma spore powder. 
         [0024]    The Ganoderma spore powder according to the present invention is sexual reproductive cells of Ganoderma, i.e., basidiospore powder. 
         [0025]    The Ganoderma spore oil according to the present invention is a fatty lipid substance extracted from Ganoderma spore powder. 
         [0026]    The alcohol according to the present invention is methanol or ethanol; the methanol may be at a concentration of 5 to 95%, and the ethanol may be at a concentration of 5 to 95%. 
         [0027]    The composition according to the present invention can be prepared into any dosage form by adding an auxiliary agent or excipient acceptable in health care products, medicaments, or products. 
         [0028]    The dosage form may be any one of a tablet, an oral liquid, a granule, a capsule, an electuary, a dripping pill, a pill, a powder, a lozenge, a fluid extract, an extract, an injection, and a syrup. 
         [0029]    The present invention provides use of a composition comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, a composition made from raw materials comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, or a composition made from Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, in the manufacture of a composition or a product for preventing and treating leucopenia induced by radiotherapy or chemotherapy. 
         [0030]    The present invention further provides use of a composition comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps and Flos Rosae Rugosae, a composition made from raw materials comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps and Flos Rosae Rugosae, or a composition made from Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps and Flos Rosae Rugosae, in the manufacture of a composition or a product for preventing and treating leucopenia induced by radiotherapy or chemotherapy. 
         [0031]    Provided is use of a composition prepared by adding any one or more components of Flos Rosae Rugosae, Ganoderma spore powder, Ganoderma spore oil, Radix Pseudostellariae, Folium Ginseng, Radix Codonopsis, and Radix Astragali to a composition comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, or to a composition made from raw materials comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, or to a composition made from Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, in the manufacture of a composition or a product for preventing and treating leucopenia induced by radiotherapy or chemotherapy. 
         [0032]    Provided is use of a composition prepared by adding any one or more components of Ganoderma spore powder, Ganoderma spore oil, Radix Pseudostellariae, Folium Ginseng, Radix Codonopsis and Radix Astragali to a composition comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps and Flos Rosae Rugosae, or to a composition made from raw materials comprising Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps and Flos Rosae Rugosae, or to a composition made from Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps and Flos Rosae Rugosae, in the manufacture of a composition or a product for preventing and treating leucopenia induced by radiotherapy or chemotherapy. 
         [0033]    A process for preparing the water and/or alcohol extracts of the raw materials for the traditional Chinese medicine composition according to the present invention comprises the steps of 
         [0000]    1) weighing out traditional Chinese drugs as the raw materials; and
 
2) extracting the raw materials under reflux with alcohol or water to obtain a liquid extract as the active ingredient, and adding auxiliary agent(s) to prepare various dosage forms.
 
         [0034]    The process for preparing the water and/or alcohol extracts of the raw materials for the traditional Chinese medicine composition according to the present invention may comprise the steps of 
         [0000]    1) weighing out traditional Chinese drugs as the raw materials, adding methanol or ethanol thereto to carry out extraction, recovering methanol or ethanol from the extraction liquid, to afford Extract I;
 
2) evaporating alcohol from the residual drugs, adding water to carry out extraction, to afford Extract II; and
 
3) combining Extract I and Extract II, carrying out filtration, concentrating the filtrate to an appropriate amount, adding pharmaceutically conventional auxiliary agent(s) to prepare a desired formulation by a pharmaceutically conventional process.
 
         [0035]    The process for preparing the water and/or alcohol extracts of the raw materials for the traditional Chinese medicine composition according to the present invention may comprise the steps of 
         [0000]    1) raw material preparation: weighing out traditional Chinese drugs as the raw materials;
 
2) extraction and concentration: soaking the Chinese drug raw materials processed in step 1) in water, then decocting several times by heating, combining the liquid extracts to carry out filtration, concentrating the filtrate to an appropriate amount, cooling the concentrate and subjecting it to high-speed centrifugation to remove impurities, and reserving the product until use;
 
3) formulation preparation: preparing the concentrate obtained in step 2), alone or together with medicinally acceptable auxiliary agent(s), into a desired formulation by a pharmaceutically conventional process;
 
wherein, in step 2) above, the raw materials are soaked for 20 to 60 min, then decocted 1 to 3 times by heating for extraction, with each decoction lasting for 1 to 2 h and having a 6 to 13-fold amount of water added.
 
         [0036]    The composition for use in the manufacture of a composition or a product for preventing and treating leucopenia induced by radiotherapy or chemotherapy according to the present invention includes health care products and medicaments, wherein the term “product” includes those not encompassed in “health care products” or “medicaments”, for example, essential oils, and pillows. 
         [0037]    In order to provide a better understanding of the spirit of the present invention, animal experiments using the composition prepared from Ganoderma, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps and the composition prepared from Ganoderma, Flos Rosae Rugosae, Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng, fermented  Cordyceps sinensis  powder and/or Cordyceps, as well as the results thereof, are described hereinafter to demonstrate the effectiveness of the composition of the present invention in prevention and treatment of leucopenia induced by radiotherapy or chemotherapy. 
         [0038]    Similarly, addition of any one or more of Ganoderma spore powder, Ganoderma spore oil, Ginseng, Radix Pseudostellariae, Radix Codonopsis, and Radix Astragali, or any combination thereof, can also lead to the same pharmacologic actions. Also, replacing Radix Panacis Quinquefolii or Radix Et Rhizoma Ginseng with Folium Ginseng, Radix Pseudostellariae, Radix Codonopsis, and/or Radix Astragali can lead to the same pharmacologic actions as well. 
     
    
     DETAILED DESCRIPTION 
     Example 1 
       [0039]    1.5 kg Radix Panacis Quinquefolii, 2.0 kg Ganoderma, and 0.33 kg Cordyceps were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above three drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 13-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered. The filtrate was concentrated to produce a clear paste, which was then spray-dried to prepare a composite powder. The composition obtained, designated as Composition 1, was used in the efficacy experiments as below. 
       Example 2 
       [0040]    1.5 kg Radix Panacis Quinquefolii, 2.0 kg Ganoderma, and 1.0 kg fermented  Cordyceps sinensis  powder were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above three drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 13-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered. The filtrate was concentrated to produce a clear paste, which was then spray-dried to prepare a composite powder. The composition obtained, Composition 2, was used in the efficacy experiments as below. 
       Example 3 
       [0041]    1.5 kg Radix Panacis Quinquefolii, 2.0 kg Ganoderma, 1.0 kg fermented  Cordyceps sinensis  powder and 0.33 kg Cordyceps were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 13-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered. The filtrate was concentrated to produce a clear paste, which was then spray-dried to prepare a composite powder. The composition obtained, Composition 3, was used in the efficacy experiments as below. 
       Example 4 
       [0042]    1.5 kg Radix Panacis Quinquefolii, 2.0 kg Ganoderma, 0.33 kg Cordyceps, and 1.5 kg Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 13-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered. The filtrate was concentrated to produce a clear paste, which was then spray-dried to prepare a composite powder. The composition obtained, Composition 4, was used in the efficacy experiments as below. 
       Example 5 
       [0043]    1.5 kg Radix Panacis Quinquefolii, 2.0 kg Ganoderma, 1.0 kg fermented  Cordyceps sinensis  powder, and 1.5 kg Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 13-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered. The filtrate was concentrated to produce a clear paste, which was then spray-dried to prepare a composite powder. The composition obtained, Composition 5, was used in the efficacy experiments as below. 
       Example 6 
       [0044]    1.5 kg Radix Panacis Quinquefolii, 2.0 kg Ganoderma, 1.0 kg fermented  Cordyceps sinensis  powder, 0.33 kg Cordyceps and 1.5 kg Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 13-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered. The filtrate was concentrated to produce a clear paste, which was then spray-dried to prepare a composite powder. The composition obtained, Composition 6, was used in the efficacy experiments as below. 
       Example 7 
       [0045]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 200 g fermented  Cordyceps sinensis  powder ( Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov), and 300 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. Each decoction lasted for 1 h with a 10-fold amount of water added. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 8 
       [0046]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 67 g Cordyceps, 200 g fermented  Cordyceps sinensis  powder ( Paecilomyces hepialli  Chen et Dai, sp.nov), and 300 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 1 h, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for tablets were added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for tablets. 
       Example 9 
       [0047]    150 g Radix Panacis Quinquefolii, 90 g fermented  Cordyceps sinensis  powder ( Hirsutella hepialid  Chen et Shen), 120 g Cordyceps, 200 g Ganoderma, and 90 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 1 h, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 10 
       [0048]    500 g Radix Et Rhizoma Ginseng, 100 g fermented  Cordyceps sinensis  powder ( Synnematium sinensis  Yin &amp; Shen), 500 g Ganoderma, and 500 g Flos Rosae Rugosae were weighed out. The Radix Et Rhizoma Ginseng and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder and Flos Rosae Rugosae were put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 11 
       [0049]    500 g Radix Panacis Quinquefolii, 100 g fermented  Cordyceps sinensis  powder ( Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov), 500 g Ganoderma, and 500 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder and Flos Rosae Rugosae were put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 12 
       [0050]    150 g Radix Panacis Quinquefolii, 120 g Cordyceps, 200 g Ganoderma, and 90 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 13 
       [0051]    150 g Radix Et Rhizoma Ginseng, 90 g fermented  Cordyceps sinensis  powder ( Gliocladium roseum  (link) Thom), 200 g Ganoderma, and 90 g Flos Rosae Rugosae were weighed out. The Radix Et Rhizoma Ginseng and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 1 h, and decocted twice by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the second decoction lasted for 1.5 h with a 10-fold amount of water added. The two liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 14 
       [0052]    150 g Radix Panacis Quinquefolii, 90 g fermented  Cordyceps sinensis  powder ( Hirsutella hepialid  Chen et Shen), 120 g Cordyceps, 200 g Ganoderma, and 90 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 1 h, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 15 
       [0053]    100 g Radix Panacis Quinquefolii, 30 g Cordyceps, 200 g Ganoderma, and 100 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 16 
       [0054]    150 g Radix Panacis Quinquefolii, 30 g fermented  Cordyceps sinensis  powder ( Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov), 200 g Ganoderma, and 100 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 1 h, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 17 
       [0055]    90 g Radix Panacis Quinquefolii, 90 g Cordyceps, 120 g Ganoderma, and 60 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. Each decoction lasted for 1 h with a 10-fold amount of water added for each. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 18 
       [0056]    90 g Radix Et Rhizoma Ginseng, 90 g Cordyceps, 120 g Ganoderma, and 60 g Flos Rosae Rugosae were weighed out. The Radix Et Rhizoma Ginseng and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. Each decoction lasted for 1 h with a 10-fold amount of water added for each. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 19 
       [0057]    90 g Radix Panacis Quinquefolii, 60 g fermented  Cordyceps sinensis  powder ( Cephalosporium sinensis  Chen sp.nov), 120 g Ganoderma, and 60 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. Each decoction lasted for 1 h with a 10-fold amount of water added for each. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 20 
       [0058]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 67 g Cordyceps, and 300 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 1 h, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for tablets were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 21 
       [0059]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 200 g fermented  Cordyceps sinensis  powder ( Paecilomyces hepialli  Chen et Dai, sp.nov), and 300 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. Each decoction lasted for 1 h with a 10-fold amount of water added for each. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 22 
       [0060]    500 g Radix Panacis Quinquefolii, 100 g Cordyceps, 500 g Ganoderma, 500 g Flos Rosae Rugosae and 500 g sporoderm-broken Ganoderma spore powder were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for tablets and the sporoderm-broken Ganoderma spore powder were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 23 
       [0061]    500 g Radix Et Rhizoma Ginseng, 100 g fermented  Cordyceps sinensis  powder ( Paecilomyces sinensis  Chen, Xiao et Shi, sp.nov), 500 g Ganoderma, 500 g Flos Rosae Rugosae and 500 g sporoderm-broken Ganoderma spore powder were weighed out. The Radix Et Rhizoma Ginseng and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder and the sporoderm-broken Ganoderma spore powder were put in a cloth bag. The above five drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for granules were added thereto and uniformly mixed; and granules were prepared by conventional processes for granules. 
       Example 24 
       [0062]    150 g Radix Panacis Quinquefolii, 90 g fermented  Cordyceps sinensis  powder ( Tolypocladium sinensis  C. lan Li), 200 g Ganoderma, 90 g Flos Rosae Rugosae and 150 g sporoderm-broken Ganoderma spore powder were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for tablets and the Ganoderma spore powder were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 25 
       [0063]    500 g Radix Panacis Quinquefolii, 100 g Cordyceps, 500 g Ganoderma, 500 g Flos Rosae Rugosae and 100 g Ganoderma spore oil were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized. Upon addition of 80% ethanol, the above four drugs were extracted twice under reflux, with each extraction lasting for 2 h, and then filtered. Ethanol was recovered from the liquid filtrate until no ethanol odor could be smelled. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for dripping pills and the Ganoderma spore oil were added thereto and uniformly mixed; and dripping pills were prepared by conventional processes for dripping pills. 
       Example 26 
       [0064]    500 g Radix Panacis Quinquefolii, 100 g fermented  Cordyceps sinensis  powder ( Synnematium sinensis  Yin &amp; Shen), 500 g Ganoderma, 500 g Flos Rosae Rugosae, 500 g sporoderm-broken Ganoderma spore powder, 100 g Ganoderma spore oil and 100 g Folium Ginseng were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for granules, the sporoderm-broken Ganoderma spore powder and the Ganoderma spore oil were added thereto and uniformly mixed; and granules were prepared by conventional processes for granules. 
       Example 27 
       [0065]    150 g Radix Panacis Quinquefolii, 90 g fermented  Cordyceps sinensis  powder ( Hirsutella hepialid  Chen et Shen), 200 g Ganoderma, 90 g Flos Rosae Rugosae and 400 g Radix Codonopsis were weighed out. The Radix Panacis Quinquefolii, Ganoderma and Radix Codonopsis were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for tablets were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 28 
       [0066]    150 g Radix Panacis Quinquefolii, 120 g Cordyceps, 200 g Ganoderma, 90 g Flos Rosae Rugosae and 400 g Radix Astragali were weighed out. The Radix Panacis Quinquefolii, Ganoderma and Radix Astragali were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 14-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agents frequently used for lozenges were added thereto and uniformly mixed, and lozenges were prepared by conventional processes for lozenges. 
       Example 29 
       [0067]    500 g Radix Panacis Quinquefolii, 50 g fermented  Cordyceps sinensis  powder ( Paecilomyces hepialli  Chen et Dai, sp.nov), 50 g fermented  Cordyceps sinensis  powder (Hirsutella  sinensis  Liu, Guo, Yu-et Zeng, sp.nov), 500 g Ganoderma, 500 g Flos Rosae Rugosae and 300 g Radix Codonopsis were weighed out. The Radix Panacis Quinquefolii, Ganoderma and Radix Codonopsis were sliced, and the fermented  Cordyceps sinensis  powders were put in a cloth bag. The above five drugs were soaked in water for 1 h, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for powder were added thereto and uniformly mixed; and powder was prepared by conventional processes for powder. 
       Example 30 
       [0068]    500 g Radix Panacis Quinquefolii, 100 g Cordyceps, 500 g Ganoderma, 500 g Flos Rosae Rugosae and 300 g Radix Astragali were weighed out. The Radix Panacis Quinquefolii, Ganoderma and Radix Astragali were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 14-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 31 
       [0069]    100 g Radix Panacis Quinquefolii, 200 g Ganoderma, 30 g Cordyceps, 3 g fermented  Cordyceps sinensis  powder (Cs-C-Q80  Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov), 100 g Flos Rosae Rugosae and 100 g Ganoderma spore powder were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and put in a cloth bag together with the Ganoderma spore powder. The above five drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for tablets were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 32 
       [0070]    100 g Radix Panacis Quinquefolii, 200 g Ganoderma, 30 g fermented  Cordyceps sinensis  powder ( Mortiscrslla hepialid  C. T.&amp; B. liu), 100 g Flos Rosae Rugosae and 100 g Ganoderma spore powder were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder and the Ganoderma spore powder were put in a cloth bag. The above five drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for pills were added thereto and uniformly mixed; and various types of pills were prepared by conventional processes for pills. 
       Example 33 
       [0071]    90 g Radix Panacis Quinquefolii, 120 g Ganoderma, 90 g Cordyceps, 60 g Flos Rosae Rugosae and 90 g Ganoderma spore oil were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced. The Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for granules and the Ganoderma spore oil were added thereto and uniformly mixed; and granules were prepared by conventional processes for granules. 
       Example 34 
       [0072]    100 g Radix Panacis Quinquefolii, 200 g Ganoderma, 30 g Cordyceps, 100 g Flos Rosae Rugosae and 200 g Radix Pseudostellariae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, auxiliary agents frequently used for soft extracts were added thereto and uniformly mixed, and a soft extract was prepared by conventional processes for soft extracts. 
       Example 35 
       [0073]    100 g Radix Panacis Quinquefolii, 200 g Ganoderma, 30 g Cordyceps, 100 g Flos Rosae Rugosae and 200 g Folium Ginseng were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agents frequently used for syrups were added thereto and uniformly mixed, and a syrup was prepared by conventional processes for syrups. 
       Example 36 
       [0074]    100 g Radix Panacis Quinquefolii, 200 g Ganoderma, 30 g fermented  Cordyceps sinensis  powder ( Mortierella  sp.), 100 g Flos Rosae Rugosae and 200 g Radix Codonopsis were weighed out. The Radix Panacis Quinquefolii, Ganoderma and Radix Codonopsis were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fined particles were made by spray drying; auxiliary agents frequently used for tablets were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 37 
       [0075]    100 g Radix Panacis Quinquefolii, 200 g Ganoderma, 30 g fermented  Cordyceps sinensis  powder ( Verticillium sinens  Wamg sp.nov), 100 g Flos Rosae Rugosae and 200 g Radix Astragali were weighed out. The Radix Panacis Quinquefolii, Ganoderma and Radix Astragali were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for capsules were added thereto and uniformly mixed; and capsules were prepared by conventional processes for capsules. 
       Example 38 
       [0076]    90 g Radix Panacis Quinquefolii, 120 g Ganoderma, 30 g fermented  Cordyceps sinensis  powder ( Cephalosporium sinensis  Chen sp.nov), 30 g fermented  Cordyceps sinensis  powder ( Synnematium sinensis  Yin &amp; Shen), 60 g Flos Rosae Rugosae and 60 g Ganoderma spore oil were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powders were put in a cloth bag. The above four drugs were soaked in water for 1 h, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 13-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for pills and the Ganoderma spore oil were added thereto and uniformly mixed; and various types of pills were prepared by conventional processes for pills. 
       Example 39 
       [0077]    90 g Radix Panacis Quinquefolii, 120 g Ganoderma, 90 g Cordyceps, 60 g Flos Rosae Rugosae, 200 g Radix Astragali, and 10 g Ganoderma spore oil were weighed out. The Radix Panacis Quinquefolii, Ganoderma and Radix Astragali were sliced, and the Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agents frequently used for syrups were added thereto and uniformly mixed, and a syrup was prepared by conventional processes for syrups. 
       Example 40 
       [0078]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 200 g fermented  Cordyceps sinensis  powder ( Scytalidium hepialii  C. L. Li), 300 g Flos Rosae Rugosae and 400 g Ganoderma spore powder were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 20 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for tablets and the Ganoderma spore powder were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 41 
       [0079]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 67 g Cordyceps, 300 g Flos Rosae Rugosae and 20 g Ganoderma spore oil were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. The above four drugs were soaked in water for 30 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for granules and the Ganoderma spore oil were added thereto and uniformly mixed; and granules were prepared by conventional processes for granules. 
       Example 42 
       [0080]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 200 g fermented  Cordyceps sinensis  powder ( Cephalosporium sinens  Chen sp.nov), 300 g Flos Rosae Rugosae and 400 g Radix Pseudostellariae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fined particles were made by spray drying; auxiliary agents frequently used for tablets were added thereto and uniformly mixed; and various types of tablets were prepared by conventional processes for tablets. 
       Example 43 
       [0081]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 67 g Cordyceps, 300 g Flos Rosae Rugosae and 400 g Radix Pseudostellariae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. The above five drugs were soaked in water for 40 min, and decocted 3 times by heating. The first decoction lasted for 2 h with a 15-fold amount of water added, and the following decoctions each lasted for 1 h with a 10-fold amount of water added for each decoction. The three liquid extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agents frequently used for syrups were added thereto and uniformly mixed, and a syrup was prepared by conventional processes for syrups. 
       Example 44 
       [0082]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 100 g fermented  Cordyceps sinensis  powder ( Chrysosporium sinens  Z. Q. liang), 100 g fermented  Cordyceps sinensis  powder ( Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov), and 300 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powders were put in a cloth bag. Upon addition of 5% methanol, the drugs were extracted twice under reflux, with each extraction lasting for 1 h. Then the liquid extracts were combined, and methanol was recovered to obtain an alcohol extract. The residual drugs were further decocted twice in water by heating. The first decoction lasted for 2 h, and the following decoction lasted for 1 h, with a 10-fold amount of water added for each decoction. The alcohol extract and water extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for granules were added thereto and uniformly mixed; and granules were prepared by conventional processes for granules. 
       Example 45 
       [0083]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 67 g Cordyceps, 20 g fermented  Cordyceps sinensis  powder ( Hirsutella sinensis  Liu, Guo, Yu-et Zeng, sp.nov), and 300 g Flos Rosae Rugosae were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. Upon addition of 75% ethanol, the drugs were extracted for 2 h under reflux, and ethanol was recovered to obtain an alcohol extract. The residual drugs were further decocted three times in water by heating, with each decoction lasting for 2 h. The alcohol extract and water extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 46 
       [0084]    300 g Radix Et Rhizoma Ginseng, 400 g Ganoderma, 200 g fermented  Cordyceps sinensis  powder ( Cephalosporium acremonium  Corda, Icones Fungorum), 300 g Flos Rosae Rugosae and 400 g Radix Codonopsis were weighed out. The Radix Et Rhizoma Ginseng, Ganoderma and Radix Codonopsis were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. Upon addition of 95% methanol, the drugs were extracted twice under reflux, with each extraction lasting for 1 h. Then the liquid extracts were combined, and methanol was recovered to obtain an alcohol extract. The residual drugs were further decocted 3 times in water by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The alcohol extract and water extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for granules were added thereto and uniformly mixed; and granules were prepared by conventional processes for granules. 
       Example 47 
       [0085]    300 g Radix Et Rhizoma Ginseng, 400 g Ganoderma, 200 g fermented  Cordyceps sinensis  powder ( Sporothrix insectorum  de Hong &amp; H. C. Evans), 300 g Flos Rosae Rugosae and 400 g Radix Codonopsis were weighed out. The Radix Et Rhizoma Ginseng and Ganoderma were sliced, and the Cordyceps was pulverized and put in a cloth bag. Upon addition of 95% ethanol, the drugs were extracted under reflux for 2 h, and ethanol was recovered to obtain an alcohol extract. The residual drugs were further decocted 3 times in water by heating, with each decoction lasting for 2 h. The alcohol extract and water extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 48 
       [0086]    300 g Radix Et Rhizoma Ginseng, 400 g Ganoderma, 67 g Cordyceps, 300 g Flos Rosae Rugosae, 300 Ganoderma spore powder and 400 g Radix Astragali were weighed out. The Radix Et Rhizoma Ginseng and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. Upon addition of 5% ethanol, the drugs were extracted under reflux for 2 h, and ethanol was recovered to obtain an alcohol extract. The residual drugs were further decocted twice in water by heating, with each decoction lasting for 2 h. The alcohol extract and water extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 49 
       [0087]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 200 g fermented  Cordyceps sinensis  powder ( Isaria farinose  (Holmsk.) Fr. Systema Mycologicum), 300 g Flos Rosae Rugosae and 400 g Radix Astragali were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the fermented  Cordyceps sinensis  powder was put in a cloth bag. Upon addition of 95% methanol, the drugs were extracted twice under reflux for 2 h, with each extraction lasting for 1 h. Then the liquid extracts were combined, and methanol was recovered to obtain an alcohol extract. The residual drugs were further decocted 3 times in water by heating. The first decoction lasted for 2 h, and the following decoctions each lasted for 1 h, with a 10-fold amount of water added for each decoction. The alcohol extract and water extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, and the impurities therein were then removed by high-speed centrifugation. A paste was made by further concentration under reduced pressure, or fine particles were made by spray drying; auxiliary agents frequently used for granules were added thereto and uniformly mixed; and granules were prepared by conventional processes for granules. 
       Example 50 
       [0088]    300 g Radix Panacis Quinquefolii, 400 g Ganoderma, 67 g Cordyceps, 300 g Flos Rosae Rugosae, and 90 g Folium Ginseng were weighed out. The Radix Panacis Quinquefolii and Ganoderma were sliced, and the Cordyceps was pulverized and then put in a cloth bag. Upon addition of 5% ethanol, the drugs were extracted under reflux for 2 h, and ethanol was recovered to obtain an alcohol extract. The residual drugs were further decocted 3 times in water by heating, with each decoction lasting for 2 h. The alcohol extract and water extracts were combined and filtered, the liquid filtrate was concentrated to an appropriate level, the liquid concentrate was left to cool down, the impurities therein were then removed by high-speed centrifugation, auxiliary agent(s) frequently used for oral liquid was added thereto and uniformly mixed, and a 20,000 ml oral liquid was prepared by conventional processes for oral liquid. 
       Example 51 
     Animal Experiment Report of Composition 1 of Example 1 in Prevention and Treatment of Leucopenia Induced by Radiotherapy or Chemotherapy 
     1. Materials and Methods 
     1.1 Sources of Samples 
       [0089]    The test drug was Composition 1 (Radix Panacis Quinquefolii, Ganoderma, and Cordyceps) provided by Jiangzhong Pharmaceutical Co. Ltd. as composite powder. 1 g dry composite powder was equivalent to 10.97 g total crude drugs. 
       1.2 Laboratory Animals 
       [0090]    Male and female mice of Kunming breed, each weighing 18 to 22 g, were provided by the Laboratory Animal Center, Jiangxi University of Traditional Chinese Medicine (Animal Certification Number: SCXK (Jiangxi) 2005-0001). 
       1.3 Primary Reagents 
       [0091]    Drug for positive control, batyl alcohol (100 mg/kg), was obtained from Shanghai Sine Yanan Pharmaceutical Co., Ltd. (batch No. 20080723). Cyclophosphamide was manufactured by Jiangsu Hengrui Medicine Co., Ltd. (batch No. 08110621). Cytarabine was manufactured by Shanghai Hualian Pharmaceuticals (batch No. 0512048). EDTA-K 2  was manufactured by Sinopharm Chemical Reagent Co., Ltd. (batch No. F20080423). 
       1.4 Primary Instruments 
       [0092]    Sysmex-2000iV blood cell analyzer (Sysmex Corporation, Japan), AR1140/C eletronic analytic balance (Ohaus (Shanghai) Corp.), microscope (Olympus), pipets (Gilson), and ELx800 Absorbance reader for ELISA (BioTek, US). 
       2. Experimental Methods 
     2.1 Effect of Composition 1 on Leucocyte Reduction in Mice Induced by Cyclophosphamide [1]   
     2.1.1 Grouping, Modeling and Dosage Regime 
       [0093]    Mice were randomly divided into 6 groups with 10 animals per group, i.e., normal control group, model control group, positive control group, and groups on low, medium and high doses of Composition 1. The low-, medium- and high-dose groups were intragastrically given Composition 1 at a dose of 2.0 g crude drug/kg, 4.0 g crude drug/kg, and 12.0 g crude drug/kg, respectively; the positive control group was intragastrically given batyl alcohol (100 mg/kg) at a dose of 0.1 ml/10 g body weight; the normal control group and the model control group were intragastrically given an equivalent volume of distilled water; and the dosage regime lasted for 15 days with one dose per day. From day 9 of intragastrical administration, all mice in each group, except the normal control group, were given cyclophosphamide at a dose of 40 mg/kg each day via subcutaneous injection for 3 consecutive days. One hour after the intragastrical administration on day 15, blood and femurs were harvested for tests. 
       2.1.2 Peripheral Leucocyte Assay 
       [0094]    40 μL blood was drawn from the tail vein of the mice and transferred into a EDTA-K 2  anticoagulative tube, into which 160 μL dilution liquid was added, and then assayed in an automatic blood cell analyzer. 
       2.1.3 Bone Marrow Nucleated Cell Count (BMC) 
       [0095]    Mice were sacrificed by cervical dislocation. The left femur was removed and flushed with a 10 ml solution of 3% acetic acid to obtain the cells in the bone marrow. The number of cells in 4 large grids on a hemacytometer was counted, and the number was multiplied by 2.5×10 4  to obtain the BMC in one femur. 
       2.1.4 Bone Marrow DNA Content Determination 
       [0096]    A 7 mm section was dissected from the middle of the right femur. The whole bone marrow was flushed into a centrifuge tube with a 10 mL solution of 0.005 mol/L CaCl 2 , placed in a refrigerator at 4° C. for 30 min, and then centrifuged at 2500 r/min for 15 min. The supernatant was discarded, and a 5 ml solution of 0.2 mol/L HClO 4  was added to the precipitate and uniformly mixed. The mixture was heated in a water bath at 90° C. for 15 min, then kept in a refrigerator overnight, and centrifuged at 2500 r/min for 10 min. The supernatant was removed and its absorbance at 268 nm was measured. 
       2.2 Effect of Composition 1 on Leucocyte Reduction in Mice Induced by Cytarabine [2]   
     2.2.1 Grouping, Modeling and Dosage Regime 
       [0097]    The grouping and dosage regime were the same as those in 2.1.1, except that, from day 7 of intragastrical administration, all animals in each group, except the normal control group, were given cytarabine at a dose of 100 mg/kg via peritoneal injection for 2 consecutive days, and from the next day the dose was switched to 50 mg/kg which was continued for another 3 consecutive days. One hour after the intragastrical administration on day 15, blood and femurs were harvested for tests. 
       2.2.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0098]    All the same as in 2.1. 
       2.3 Effect of Composition 1 on Leucocyte Reduction in Mice Induced by X-Ray Radiation [3]   
     2.3.1 Grouping, Modeling and Dosage Regime 
       [0099]    The grouping and dosage regime were the same as those in 2.1.1 except that all groups other than the normal group were subjected to systematic radiation once under low-energy X ray from a medical linear accelerator to establish models, wherein the skin-to-source distance was 100 cm, the radiation dose was 4.0 Gy/min and the radiation duration was 4 min One hour after the intragastrical administration on day 8 and day 15, blood and femurs were harvested for tests. 
       2.3.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0100]    All the same as in 2.1. 
       2.4 Statistic Method 
       [0101]    Data was processed by SPSS15.0 using a one-way ANOVA analysis. P&lt;0.05 is considered as significant difference. P&lt;0.01 is considered as highly significant difference. 
       3. Results 
     3.1 Effect of Composition 1 on Leucocyte Reduction in Mice Induced by Cyclophosphamide 
       [0102]    Test results are shown in Tables 1 and 2. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 1 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 1 low-dose group, indicating that Composition 1 can resist leucocyte reduction in mice induced by cyclophosphamide and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Effect of Composition 1 on peripheral leucocyte reduction in mice induced 
               
               
                 by cyclophosphamide (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 8.19 ± 1.96 
                 8.37 ± 1.54 
                 8.28 ± 1.77 
               
               
                 Model control group 
                 0.0 
                 10 
                 8.08 ± 1.58 
                 3.46 ± 1.09** 
                 4.25 ± 1.41** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 8.20 ± 2.19 
                 3.55 ± 1.21** 
                 6.20 ± 1.82 ▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 8.22 ± 1.54 
                 3.48 ± 1.36** 
                 5.15 ± 1.62 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 8.32 ± 1.73 
                 3.50 ± 1.02** 
                 6.40 ± 1.92 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 8.42 ± 1.89 
                 3.29 ± 1.13** 
                 6.92 ± 1.87  ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 2 
               
             
             
               
                   
               
               
                 Effect of Composition 1 on the contents of bone marrow BCM and DNA in 
               
               
                 mice injected with cyclophosphamide (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 16.01 ± 2.42 
                 0.913 ± 0.276 
               
               
                 Model control group 
                 0.0 
                 10 
                  10.9 ± 2.63** 
                 0.422 ± 0.108** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.44 ± 2.36 ▴   
                 0.610 ± 0.138 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 12.18 ± 1.97 
                 0.523 ± 0.142 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 13.56 ± 2.78 ▴   
                 0.602 ± 0.157 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.49 ± 2.95 ▴▴   
                 0.680 ± 0.176 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.2 Effect of Composition 1 on Leucocyte Reduction in Mice Induced by Cytarabine 
       [0103]    Test results are shown in Tables 3 and 4. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 1 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 1 low-dose group, indicating that Composition 1 can resist leucocyte induction in mice induced by cytarabine and improve the hematopoietic function of the bone marrow thereof. 
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                 TABLE 3 
               
             
             
               
                   
               
               
                 Effect of Composition 1 on peripheral leucocyte reduction in mice induced 
               
               
                 by cytarabine (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude  
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 8.45 ± 1.87 
                 8.59 ± 1.90 
                 8.74 ± 2.13 
               
               
                 Model control group 
                 0.0 
                 10 
                 8.62 ± 1.92 
                 3.67 ± 1.20** 
                 4.47 ± 1.26** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 8.50 ± 2.32 
                 3.80 ± 1.03** 
                 6.54 ± 1.93 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 8.33 ± 1.69 
                 3.54 ± 1.21** 
                 5.44 ± 1.09 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 8.45 ± 1.85 
                 3.76 ± 1.09** 
                 6.07 ± 1.23 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 8.29 ± 2.08 
                 3.48 ± 1.03 ** 
                 6.87 ± 1.50 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 4 
               
             
             
               
                   
               
               
                 Effect of Composition 1 on the contents of bone marrow BCM and DNA in 
               
               
                 mice injected with cytarabine (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 15.38 ± 2.60 
                 0.945 ± 0.292 
               
               
                 Model control group 
                 0.0 
                 10 
                  9.86 ± 2.17** 
                 0.460 ± 0.127** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 12.53 ± 2.24 ▴   
                 0.634 ± 0.130 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 11.36 ± 1.85 
                 0.544 ± 0.153 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 12.49 ± 2.52 ▴   
                 0.627 ± 0.136 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 13.26 ± 2.88 ▴▴   
                 0.709 ± 0.190 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.3 Effect of Composition 1 on Leucocyte Reduction in Mice Induced by X-Ray Radiation 
       [0104]    Test results are shown in Tables 5 and 6. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 1 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 1 low-dose group, indicating that Composition 1 can resist leucocyte induction in mice induced by X-ray radiation and improve the hematopoietic function of the bone marrow thereof. 
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                 TABLE 5 
               
             
             
               
                   
               
               
                 Effect of Composition 1 on peripheral leucocyte reduction in mice induced 
               
               
                 by X-ray radiation (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g 
                   
                   
                   
                   
               
               
                   
                 crude 
                 Number of 
                 WBC before 
                 WBC on day 8 
                 WBC on day 15 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 after treatment 
                 after treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 8.92 ± 2.05 
                 9.05 ± 2.54 
                 9.12 ± 2.30 
               
               
                 Model control group 
                 0.0 
                 10 
                 8.85 ± 1.80 
                 3.89 ± 1.14** 
                 5.08 ± 1.60** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 8.74 ± 2.21 
                 3.66 ± 1.05** 
                 6.92 ± 1.98 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 8.90 ± 1.94 
                 3.82 ± 1.12** 
                 5.75 ± 1.44 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 8.68 ± 1.80 
                 3.91 ± 1.30** 
                 6.73 ± 1.29 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 8.79 ± 1.78 
                 3.76 ± 1.22** 
                 7.47 ± 1.41 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 6 
               
             
             
               
                   
               
               
                 Effect of Composition 1 on the contents of bone marrow BCM and DNA in 
               
               
                 mice after X-ray radiation (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 15.85 ± 2.60 
                 0.978 ± 0.285 
               
               
                 Model control group 
                 0.0 
                 10 
                 10.42 ± 2.31** 
                 0.480 ± 0.124** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.20 ± 2.02 ▴   
                 0.637 ± 0.150 ▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 11.94 ± 1.67 
                 0.563 ± 0.121 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 13.08 ± 2.62 ▴▴   
                 0.653 ± 0.140 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.15 ± 2.76 ▴▴   
                 0.701 ± 0.192 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       4. Conclusion 
       [0105]    The animal experiments demonstrate that Composition 1 obtained in Example 1 can resist leucocyte reduction in mice induced by cyclophosphamide, cytarabine and X-ray radiation and can improve the hematopoietic function of the bone marrow thereof, indicating that Composition 1 may be effective in preventing and treating leucopenia induced by radiotherapy and chemotherapy. 
       Example 52 
     Animal Experiment Report of Composition 2 Obtained in Example 2 in Prevention and Treatment of Leucopenia Induced by Radiotherapy or Chemotherapy 
     1. Materials and Methods 
     1.1 Sources of Samples 
       [0106]    The test drug was Composition 2 (Radix Panacis Quinquefolii, Ganoderma, and fermented  Cordyceps sinensis  powder) provided by Jiangzhong Pharmaceutical Co. Ltd. as composite powder. 1 g dry composite powder was equivalent to 11.41 g total crude drugs. 
       1.2 Laboratory Animals 
       [0107]    The same as in Example 51. 
       1.3 Primary Reagents 
       [0108]    The same as in Example 51. 
       1.4 Primary Instruments 
       [0109]    The same as in Example 51. 
       2. Experimental Methods 
     2.1 Effect of Composition 2 on Leucocyte Reduction in Mice Induced by Cyclophosphamide [1]   
     2.1.1 Grouping, Modeling and Dosage Regime 
       [0110]    Mice were randomly divided into 6 groups with 10 animals per group, i.e., normal control group, model control group, positive control group, and groups on low, medium and high doses of Composition 2. The low-, medium- and high-dose groups were intragastrically given Composition 2 at a dose of 2.0 g crude drug/kg, 4.0 g crude drug/kg, and 12.0 g crude drug/kg, respectively; the positive control group was intragastrically given batyl alcohol (100 mg/kg) at a dose of 0.1 ml/10 g body weight; the normal control group and the model control group were intragastrically given an equivalent volume of distilled water; and the dosage regime lasted for 15 days with one dose per day. From day 9 of intragastrical administration, all mice in each group, except the normal control group, were given cyclophosphamide at a dose of 40 mg/kg each day via subcutaneous injection for 3 consecutive days. One hour after the intragastrical administration on day 15, blood and femurs were harvested for tests. 
       2.1.2 Peripheral Leucocyte Assay 
       [0111]    The same as in Example 51. 
       2.1.3 Bone Marrow Nucleated Cell Count (BMC) 
       [0112]    The same as in Example 51. 
       2.1.4 Bone Marrow DNA Content Determination 
       [0113]    The same as in Example 51. 
       2.2 Effect of Composition 2 on Leucocyte Reduction in Mice Induced by Cytarabine [2]   
     2.2.1 Grouping, Modeling and Dosage Regime 
       [0114]    The same as in Example 51. 
       2.2.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0115]    The same as in 2.1. 
       2.3 Effect of Composition 2 on Leucocyte Reduction in Mice Induced by X-Ray Radiation [3]   
     2.3.1 Grouping, Modeling and Dosage Regime 
       [0116]    The same as in Example 51. 
       2.3.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0117]    The same as in 2.1. 
       2.4 Statistic Method 
       [0118]    The same as in Example 51. 
       3. Results 
     3.1 Effect of Composition 2 on Leucocyte Reduction in Mice Induced by Cyclophosphamide 
       [0119]    Test results are shown in Tables 1 and 2. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 2 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 2 low-dose group, indicating that Composition 2 can resist leucocyte reduction in mice induced by cyclophosphamide and improve the hematopoietic function of the bone marrow thereof. 
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                 TABLE 1 
               
             
             
               
                   
               
               
                 Effect of Composition 2 on peripheral leucocyte reduction in mice induced 
               
               
                 by cyclophosphamide (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g 
                 Number  
                   
                   
                   
               
               
                   
                 crude 
                 of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 9.21 ± 1.75 
                 9.44 ± 1.72 
                 9.35 ± 1.94 
               
               
                 Model control group 
                 0.0 
                 10 
                 9.33 ± 2.30 
                 4.75 ± 1.22** 
                 5.26 ± 1.72** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 9.42 ± 2.12 
                 4.64 ± 1.30** 
                 7.34 ± 1.59 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 9.20 ± 1.89 
                 4.60 ± 1.41** 
                 6.24 ± 1.88 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 9.26 ± 1.96 
                 4.55 ± 1.25** 
                 7.21 ± 1.62 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 9.06 ± 1.64 
                 4.39 ± 1.32** 
                 7.81 ± 1.90 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 2 
               
             
             
               
                   
               
               
                 Effect of Composition 2 on the contents of bone marrow BCM and DNA in 
               
               
                 mice injected with cyclophosphamide (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 14.39 ± 2.46 
                 0.928 ± 0.264 
               
               
                 Model control group 
                 0.0 
                 10 
                  9.60 ± 2.72** 
                 0.440 ± 0.125** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 11.87 ± 2.08 ▴▴   
                 0.629 ± 0.150 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 10.95 ± 2.14 
                 0.537 ± 0.132 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 12.62 ± 2.45 ▴   
                 0.624 ± 0.166 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 13.42 ± 2.80 ▴▴   
                 0.699 ± 0.169 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.2 Effect of Composition 2 on Leucocyte Reduction in Mice Induced by Cytarabine 
       [0120]    Test results are shown in Tables 3 and 4. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 2 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 2 low-dose group, indicating that Composition 2 can resist leucocyte induction in mice induced by cytarabine and improve the hematopoietic function of the bone marrow thereof. 
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                 TABLE 3 
               
             
             
               
                   
               
               
                 Effect of Composition 2 on peripheral leucocyte reduction in mice induced 
               
               
                 by cytarabine (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 8.70 ± 1.76 
                 8.90 ± 1.44 
                 8.94 ± 2.20 
               
               
                 Model control group 
                 0.0 
                 10 
                 8.92 ± 1.88 
                 4.58 ± 1.36** 
                 4.70 ± 1.16** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 8.68 ± 2.03 
                 4.67 ± 1.27** 
                 6.85 ± 1.70 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 8.78 ± 1.96 
                 4.80 ± 1.08** 
                 5.73 ± 1.23 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 8.85 ± 2.13 
                 4.38 ± 1.14** 
                 6.17 ± 1.23 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 8.69 ± 2.01 
                 4.62 ± 1.18** 
                 7.38 ± 2.44 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 4 
               
             
             
               
                   
               
               
                 Effect of Composition 2 on the contents of bone marrow BCM and DNA in 
               
               
                 mice injected with cytarabine (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 15.92 ± 2.60 
                 0.993 ± 0.267 
               
               
                 Model control group 
                 0.0 
                 10 
                 10.61 ± 2.82** 
                 0.503 ± 0.161** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.95 ± 2.77 ▴   
                 0.684 ± 0.152 ▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 11.90 ± 2.20 
                 0.590 ± 0.142 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 13.44 ± 2.70 ▴   
                 0.676 ± 0.128 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.08 ± 1.92 ▴▴   
                 0.733 ± 0.181 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.3 Effect of Composition 2 on Leucocyte Reduction in Mice Induced by X-Ray Radiation 
       [0121]    Test results are shown in Tables 5 and 6. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 2 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 2 low-dose group, indicating that Composition 2 can resist leucocyte induction in mice induced by X-ray radiation and improve the hematopoietic function of the bone marrow thereof. 
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                 TABLE 5 
               
             
             
               
                   
               
               
                 Effect of Composition 2 on peripheral leucocyte reduction in mice induced 
               
               
                 by X-ray radiation (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g 
                 Number  
                   
                   
                   
               
               
                   
                 crude 
                 of 
                 WBC before 
                 WBC on day 8 
                 WBC on day 15 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 after treatment 
                 after treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 10.24 ± 2.05 
                 10.02 ± 2.32 
                 10.12 ± 2.41 
               
               
                 Model control group 
                 0.0 
                 10 
                 10.47 ± 2.14 
                  5.38 ± 1.33** 
                  5.16 ± 1.44** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 10.16 ± 2.30 
                  5.60 ± 1.28** 
                  7.04 ± 1.87 ▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 10.58 ± 1.89 
                  5.52 ± 1.19** 
                  6.28 ± 1.50 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 10.32 ± 1.62 
                  5.24 ± 1.21** 
                  6.94 ± 1.66 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 10.27 ± 1.33 
                  5.46 ± 1.20** 
                  7.26 ± 1.48 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 6 
               
             
             
               
                   
               
               
                 Effect of Composition 2 on the contents of bone marrow BCM and DNA in 
               
               
                 mice after X-ray radiation (  x  ± s) 
               
             
          
           
               
                 Groups 
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                   
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 14.32 ± 2.78 
                 0.916 ± 0.274 
               
               
                 Model control group 
                 0.0 
                 10 
                  9.15 ± 2.07** 
                 0.425 ± 0.108** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 12.43 ± 2.29 ▴   
                 0.589 ± 0.123 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 11.03 ± 1.90 
                 0.514 ± 0.119 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 12.20 ± 2.82 ▴▴   
                 0.609 ± 0.126 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 13.09 ± 2.55 ▴▴   
                 0.685 ± 0.187 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       4. Conclusion 
       [0122]    The animal experiments demonstrate that Composition 2 obtained in Example 2 can resist leucocyte reduction in mice induced by cyclophosphamide, cytarabine and X-ray radiation and can improve the hematopoietic function of the bone marrow thereof, indicating that Composition 2 may be effective in preventing and treating leucopenia induced by radiotherapy and chemotherapy. 
       Example 53 
     Animal Experiment Report of Composition 3 Obtained in Example 3 in Prevention and Treatment of Leucopenia Induced by Radiotherapy or Chemotherapy 
     1. Materials and Methods 
     1.1 Sources of Samples 
       [0123]    The test drug was Composition 3 (Radix Panacis Quinquefolii, Ganoderma, fermented  Cordyceps sinensis  powder and Cordyceps) provided by Jiangzhong Pharmaceutical Co. Ltd. as composite powder. 1 g dry composite powder was equivalent to 12.39 g total crude drugs. 
       1.2 Laboratory Animals 
       [0124]    The same as in Example 51. 
       1.3 Primary Reagents 
       [0125]    The same as in Example 51. 
       1.4 Primary Instruments 
       [0126]    The same as in Example 51. 
       2. Experimental Methods 
     2.1 Effect of Composition 3 on Leucocyte Reduction in Mice Induced by Cyclophosphamide [1]   
     2.1.1 Grouping, modeling and dosage regime 
       [0127]    Mice were randomly divided into 6 groups with 10 animals per group, i.e., normal control group, model control group, positive control group, and groups on low, medium and high doses of Composition 3. The low-, medium- and high-dose groups were intragastrically given Composition 3 at a dose of 2.0 g crude drug/kg, 4.0 g crude drug/kg, and 12.0 g crude drug/kg, respectively; the positive control group was intragastrically given batyl alcohol (100 mg/kg) at a dose of 0.1 ml/10 g body weight; the normal control group and the model control group were intragastrically given an equivalent volume of distilled water; and the dosage regime lasted for 15 days with one dose per day. From day 9 of intragastrical administration, all mice in each group, except the normal control group, were given cyclophosphamide at a dose of 40 mg/kg each day via subcutaneous injection for 3 consecutive days. One hour after the intragastrical administration on day 15, blood and femurs were harvested for tests. 
       2.1.2 Peripheral Leucocyte Assay 
       [0128]    The same as in Example 51. 
       2.1.3 Bone Marrow Nucleated Cell Count (BMC) 
       [0129]    The same as in Example 51. 
       2.1.4 Bone Marrow DNA Content Determination 
       [0130]    The same as in Example 51. 
       2.2 Effect of Composition 3 on Leucocyte Reduction in Mice Induced by Cytarabine [2]   
     2.2.1 Grouping, Modeling and Dosage Regime 
       [0131]    The same as in Example 51. 
       2.2.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0132]    The same as in 2.1. 
       2.3 Effect of Composition 3 on Leucocyte Reduction in Mice Induced by X-Ray Radiation [3]   
     2.3.1 Grouping, Modeling and Dosage Regime 
       [0133]    The same as in Example 51. 
       2.3.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0134]    The same as in 2.1. 
       2.4 Statistic method 
       [0135]    The same as in Example 51. 
       3. Results 
     3.1 Effect of Composition 3 on Leucocyte Reduction in Mice Induced by Cyclophosphamide 
       [0136]    Test results are shown in Tables 1 and 2. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 3 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 3 low-dose group, indicating that Composition 3 can resist leucocyte reduction in mice induced by cyclophosphamide and improve the hematopoietic function of the bone marrow thereof. 
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                 TABLE 1 
               
             
             
               
                   
               
               
                 Effect of Composition 3 on peripheral leucocyte reduction in mice induced 
               
               
                 by cyclophosphamide (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 9.55 ± 1.90 
                 9.82 ± 2.04 
                 9.69 ± 1.77 
               
               
                 Model control group 
                 0.0 
                 10 
                 9.70 ± 2.04 
                 5.03 ± 1.28** 
                 5.20 ± 1.76** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 9.27 ± 2.21 
                 5.25 ± 1.30** 
                 7.56 ± 1.80 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 9.80 ± 1.96 
                 5.18 ± 1.41** 
                 6.50 ± 1.62 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 9.64 ± 1.88 
                 5.23 ± 1.25** 
                 7.24 ± 1.88 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 9.55 ± 1.79 
                 5.40 ± 1.32** 
                 7.94 ± 1.94 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 2 
               
             
             
               
                   
               
               
                 Effect of Composition 3 on the contents of bone marrow BCM and DNA in 
               
               
                 mice injected with cyclophosphamide (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 15.42 ± 2.86 
                 0.877 ± 0.212 
               
               
                 Model control group 
                 0.0 
                 10 
                 10.88 ± 2.13** 
                 0.438 ± 0.107** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.65 ± 2.42 ▴   
                 0.625 ± 0.162 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 12.80 ± 2.33 
                 0.584 ± 0.140 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 13.23 ± 2.72 ▴   
                 0.635 ± 0.172 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 13.99 ± 1.93 ▴▴   
                 0.684 ± 0.155 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.2 Effect of Composition 3 on Leucocyte Reduction in Mice Induced by Cytarabine 
       [0137]    Test results are shown in Tables 3 and 4. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 3 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 3 low-dose group, indicating that Composition 3 can resist leucocyte induction in mice induced by cytarabine and improve the hematopoietic function of the bone marrow thereof. 
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                 TABLE 3 
               
             
             
               
                   
               
               
                 Effect of Composition 3 on peripheral leucocyte reduction in mice induced 
               
               
                 by cytarabine (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 9.22 ± 1.80 
                 9.30 ± 1.86 
                 9.51 ± 2.26 
               
               
                 Model control group 
                 0.0 
                 10 
                 9.45 ± 1.92 
                 4.83 ± 1.51** 
                 4.73 ± 1.34** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 9.30 ± 2.14 
                 4.90 ± 1.43** 
                 7.10 ± 1.88 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 9.26 ± 1.54 
                 4.84 ± 1.26** 
                 5.66 ± 1.45 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 9.17 ± 2.07 
                 4.64 ± 1.33** 
                 6.52 ± 1.48 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 9.23 ± 2.21 
                 4.91 ± 1.27** 
                 7.29 ± 1.90 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
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                 TABLE 4 
               
             
             
               
                   
               
               
                 Effect of Composition 3 on the contents of bone marrow BCM and DNA in 
               
               
                 mice injected with cytarabine (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 15.60 ± 2.72 
                 0.980 ± 0.209 
               
               
                 Model control group 
                 0.0 
                 10 
                 10.37 ± 2.43** 
                 0.524 ± 0.142** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.64 ± 2.19 ▴   
                 0.713 ± 0.138 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 11.53 ± 2.34 
                 0.621 ± 0.151 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 13.10 ± 2.56 ▴   
                 0.693 ± 0.136 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.21 ± 2.80 ▴▴   
                 0.748 ± 0.175 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.3 Effect of Composition 3 on Leucocyte Reduction in Mice Induced by X-Ray Radiation 
       [0138]    Test results are shown in Tables 5 and 6. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 3 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 3 low-dose group, indicating that Composition 3 can resist leucocyte induction in mice induced by X-ray radiation and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Effect of Composition 3 on peripheral leucocyte reduction in mice induced 
               
               
                 by X-ray radiation (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g 
                 Number  
                   
                   
                   
               
               
                 Groups 
                 crude 
                 of 
                 WBC before 
                 WBC on day 8 
                 WBC on day 15 
               
               
                   
                 drug/kg) 
                 animals 
                 modeling 
                 after treatment  
                 after treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 10.54 ± 2.18 
                 10.16 ± 2.05 
                 10.43 ± 2.35 
               
               
                 Model control group 
                 0.0 
                 10 
                 10.60 ± 2.09 
                  5.92 ± 1.38** 
                  5.88 ± 1.70** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 10.88 ± 2.24 
                  5.87 ± 1.62** 
                  7.49 ± 1.52 ▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 10.27 ± 1.92 
                  5.65 ± 1.40** 
                  6.89 ± 1.33 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 10.58 ± 2.30 
                  5.80 ± 1.53** 
                  7.61 ± 1.53 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 10.37 ± 2.33 
                  5.74 ± 1.38** 
                  8.22 ± 1.60 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Effect of Composition 3 on the contents of bone marrow BCM and DNA in 
               
               
                 mice after X-ray radiation (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 16.50 ± 2.80 
                 0.998 ± 0.258 
               
               
                 Model control group 
                 0.0 
                 10 
                 11.27 ± 2.34** 
                 0.520 ± 0.120** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.69 ± 2.65 ▴   
                 0.723 ± 0.135 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 13.22 ± 2.18 
                 0.626 ± 0.127 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 14.40 ± 2.43 ▴▴   
                 0.734 ± 0.178 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 15.17 ± 2.26 ▴▴   
                 0.765 ± 0.166 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       4. Conclusion 
       [0139]    The animal experiments demonstrate that Composition 3 obtained in Example 3 can resist leucocyte reduction in mice induced by cyclophosphamide, cytarabine and X-ray radiation and can improve the hematopoietic function of the bone marrow thereof, indicating that Composition 3 may be effective in preventing and treating leucopenia induced by radiotherapy and chemotherapy. 
       Example 54 
     Animal Experiment Report of Composition 4 Obtained in Example 4 in Prevention and Treatment of Leucopenia Induced by Radiotherapy or Chemotherapy 
     1. Materials and Methods 
     1.1 Sources of Samples 
       [0140]    The test drug was Composition 4 (Radix Panacis Quinquefolii, Ganoderma, Cordyceps, and Flos Rosae Rugosae) provided by Jiangzhong Pharmaceutical Co. Ltd. as composite powder. 1 g dry composite powder was equivalent to 12.19 g total crude drugs. 
       1.2 Laboratory Animals 
       [0141]    The same as in Example 51. 
       1.3 Primary Reagents 
       [0142]    The same as in Example 51. 
       1.4 Primary Instruments 
       [0143]    The same as in Example 51. 
       2. Experimental Methods 
     2.1 Effect of Composition 4 on Leucocyte Reduction in Mice Induced by Cyclophosphamide [1]   
     2.1.1 Grouping, Modeling and Dosage Regime 
       [0144]    Mice were randomly divided into 6 groups with 10 animals per group, i.e., normal control group, model control group, positive control group, and groups on low, medium and high doses of Composition 4. The low-, medium- and high-dose groups were intragastrically given Composition 4 at a dose of 2.0 g crude drug/kg, 4.0 g crude drug/kg, and 12.0 g crude drug/kg, respectively; the positive control group was intragastrically given batyl alcohol (100 mg/kg) at a dose of 0.1 ml/10 g body weight; the normal control group and the model control group were intragastrically given an equivalent volume of distilled water; and the dosage regime lasted for 15 days with one dose per day. From day 9 of intragastrical administration, all mice in each group, except the normal control group, were given cyclophosphamide at a dose of 40 mg/kg each day via subcutaneous injection for 3 consecutive days. One hour after the intragastrical administration on day 15, blood and femurs were harvested for tests. 
       2.1.2 Peripheral Leucocyte Assay 
       [0145]    The same as in Example 51. 
       2.1.3 Bone Marrow Nucleated Cell Count (BMC) 
       [0146]    The same as in Example 51. 
       2.1.4 Bone Marrow DNA Content Determination 
       [0147]    The same as in Example 51. 
       2.2 Effect of Composition 4 on Leucocyte Reduction in Mice Induced by Cytarabine [2]   
     2.2.1 Grouping, Modeling and Dosage Regime 
       [0148]    The same as in Example 51. 
       2.2.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0149]    The same as in 2.1. 
       2.3 Effect of Composition 4 on Leucocyte Reduction in Mice Induced by X-Ray Radiation [3]   
     2.3.1 Grouping, Modeling and Dosage Regime 
       [0150]    The same as in Example 51. 
       2.3.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0151]    The same as in 2.1. 
       2.4 Statistic Method 
       [0152]    The same as in Example 51. 
       3. Results 
     3.1 Effect of Composition 4 on Leucocyte Reduction in Mice Induced by Cyclophosphamide 
       [0153]    Test results are shown in Tables 1 and 2. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 4 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 4 low-dose group, indicating that Composition 4 can resist leucocyte reduction in mice induced by cyclophosphamide and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Effect of Composition 4 on peripheral leucocyte reduction in mice induced 
               
               
                 by cyclophosphamide (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 10.43 ± 1.88 
                 10.81 ± 2.61 
                 10.60 ± 2.32 
               
               
                 Model control group 
                 0.0 
                 10 
                 10.56 ± 2.14 
                  5.77 ± 1.62** 
                  5.96 ± 1.78** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 10.29 ± 2.30 
                  6.13 ± 1.47** 
                  8.23 ± 1.54 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 10.74 ± 2.11 
                  5.86 ± 1.38** 
                  7.48 ± 1.86 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 10.27 ± 2.26 
                  6.08 ± 1.62** 
                  8.10 ± 2.03 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 10.50 ± 2.09 
                  5.94 ± 1.55** 
                  8.52 ± 1.90 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Effect of Composition 4 on the contents of bone marrow BCM and DNA in 
               
               
                 mice injected with cyclophosphamide (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 16.60 ± 2.92 
                 0.956 ± 0.233 
               
               
                 Model control group 
                 0.0 
                 10 
                 11.86 ± 2.21** 
                 0.544 ± 0.132** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 14.73 ± 2.04 ▴   
                 0.730 ± 0.150 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 13.93 ± 2.26 
                 0.660 ± 0.138 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 14.35 ± 2.51 ▴   
                 0.752 ± 0.169 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.90 ± 1.88 ▴▴   
                 0.786 ± 0.172 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.2 Effect of Composition 4 on Leucocyte Reduction in Mice Induced by Cytarabine 
       [0154]    Test results are shown in Tables 3 and 4. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 4 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 4 low-dose group, indicating that Composition 4 can resist leucocyte induction in mice induced by cytarabine and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Effect of Composition 4 on peripheral leucocyte reduction in mice induced 
               
               
                 by cytarabine (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
             
          
           
               
                 Normal control group 
                 0.0 
                 10 
                 8.16 ± 1.76 
                 8.36 ± 1.58 
                 8.47 ± 1.95 
               
               
                 Model control group 
                 0.0 
                 10 
                 8.50 ± 1.54 
                 4.51 ± 1.61** 
                 4.48 ± 1.58** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 8.28 ± 1.62 
                 4.16 ± 1.09** 
                 7.26 ± 1.90 ▴▴   
               
               
                 Test drug low-dose group 
                 2.0 
                 10 
                 8.53 ± 2.08 
                 4.66 ± 1.17** 
                 5.54 ± 1.75 
               
               
                 Test drug medium-dose group 
                 4.0 
                 10 
                 8.60 ± 2.11 
                 4.35 ± 1.25** 
                 6.71 ± 1.98 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 8.29 ± 1.65 
                 4.27 ± 1.41** 
                 7.16 ± 1.22 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Effect of Composition 4 on the contents of bone marrow  
               
               
                 BCM and DNA in mice injected with cytarabine (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 17.21 ± 2.96 
                 1.052 ± 0.226 
               
               
                 Model control group 
                  0.0 
                 10 
                 11.16 ± 2.88** 
                 0.673 ± 0.145** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 14.32 ± 2.65 ▴   
                 0.879 ± 0.169 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 13.66 ± 2.78 
                 0.802 ± 0.184 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 14.50 ± 2.90 ▴   
                 0.868 ± 0.166 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 15.63 ± 2.67 ▴▴   
                 0.944 ± 0.190 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.3 Effect of Composition 4 on Leucocyte Reduction in Mice Induced by X-Ray Radiation 
       [0155]    Test results are shown in Tables 5 and 6. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 4 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 4 low-dose group, indicating that Composition 4 can resist leucocyte induction in mice induced by X-ray radiation and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Effect of Composition 4 on peripheral leucocyte reduction  
               
               
                 in mice induced by X-ray radiation (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                   
                   
                   
                 WBC  
                 WBC  
               
               
                   
                 Dose  
                 Number  
                 WBC  
                 on day 8 
                 on day 15 
               
               
                   
                 (g crude 
                 of 
                 before 
                 after  
                 after  
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 treatment 
                 treatment 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 9.67 ± 2.26 
                 9.33 ± 2.15 
                 9.46 ± 2.20 
               
               
                 Model control group 
                  0.0 
                 10 
                 9.36 ± 2.17 
                 5.71 ± 1.20** 
                 5.60 ± 1.45** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 9.42 ± 2.05 
                 5.80 ± 1.44** 
                 7.88 ± 1.61 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 9.57 ± 1.77 
                 5.24 ± 1.07** 
                 6.76 ± 1.22 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 9.83 ± 2.08 
                 5.33 ± 1.18** 
                 7.40 ± 1.48 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 9.47 ± 2.19 
                 5.52 ± 1.24** 
                 8.36 ± 1.55 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Effect of Composition 4 on the contents of bone marrow  
               
               
                 BCM and DNA in mice after X-ray radiation (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 17.06 ± 2.96 
                 0.972 ± 0.224 
               
               
                 Model control group 
                  0.0 
                 10 
                 12.11 ± 2.03** 
                 0.541 ± 0.109** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 14.57 ± 2.57 ▴   
                 0.760 ± 0.147 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 14.06 ± 2.42 
                 0.644 ± 0.122 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 15.31 ± 2.60 ▴▴   
                 0.752 ± 0.103 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 16.02 ± 2.33 ▴▴   
                 0.786 ± 0.148 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       4. Conclusion 
       [0156]    The animal experiments demonstrate that Composition 4 obtained in Example 4 can resist leucocyte reduction in mice induced by cyclophosphamide, cytarabine and X-ray radiation and can improve the hematopoietic function of the bone marrow thereof, indicating that Composition 4 may be effective in preventing and treating leucopenia induced by radiotherapy and chemotherapy. 
       Example 55 
     Animal Experiment Report of Composition 5 Obtained in Example 5 in Prevention and Treatment of Leucopenia Induced by Radiotherapy or Chemotherapy 
     1. Materials and Methods 
     1.1 Sources of Samples 
       [0157]    The test drug was Composition 5 (Radix Panacis Quinquefolii, Ganoderma, fermented  Cordyceps sinensis  powder, and Flos Rosae Rugosae) provided by Jiangzhong Pharmaceutical Co. Ltd. as composite powder. 1 g dry composite powder was equivalent to 12.56 g total crude drugs. 
       1.2 Laboratory Animals 
       [0158]    The same as in Example 51. 
       1.3 Primary Reagents 
       [0159]    The same as in Example 51. 
       1.4 Primary Instruments 
       [0160]    The same as in Example 51. 
       2. Experimental Methods 
     2.1 Effect of Composition 5 on Leucocyte Reduction in Mice Induced by Cyclophosphamide [1]   
     2.1.1 Grouping, Modeling and Dosage Regime 
       [0161]    Mice were randomly divided into 6 groups with 10 animals per group, i.e., normal control group, model control group, positive control group, and groups on low, medium and high doses of Composition 5. The low-, medium- and high-dose groups were intragastrically given Composition 5 at a dose of 2.0 g crude drug/kg, 4.0 g crude drug/kg, and 12.0 g crude drug/kg, respectively; the positive control group was intragastrically given batyl alcohol (100 mg/kg) at a dose of 0.1 ml/10 g body weight; the normal control group and the model control group were intragastrically given an equivalent volume of distilled water; and the dosage regime lasted for 15 days with one dose per day. From day 9 of intragastrical administration, all mice in each group, except the normal control group, were given cyclophosphamide at a dose of 40 mg/kg each day via subcutaneous injection for 3 consecutive days. One hour after the intragastrical administration on day 15, blood and femurs were harvested for tests. 
       2.1.2 Peripheral Leucocyte Assay 
       [0162]    The same as in Example 51. 
       2.1.3 Bone Marrow Nucleated Cell Count (BMC) 
       [0163]    The same as in Example 51. 
       2.1.4 Bone Marrow DNA Content Determination 
       [0164]    The same as in Example 51. 
       2.2 Effect of Composition 5 on Leucocyte Reduction in Mice Induced by Cytarabine [2]   
     2.2.1 Grouping, Modeling and Dosage Regime 
       [0165]    The same as in Example 51. 
       2.2.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0166]    The same as in 2.1. 
       2.3 Effect of Composition 5 on Leucocyte Reduction in Mice Induced by X-Ray Radiation [3]   
     2.3.1 Grouping, Modeling and Dosage Regime 
       [0167]    The same as in Example 51. 
       2.3.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0168]    The same as in 2.1. 
       2.4 Statistic Method 
       [0169]    The same as in Example 51. 
       3. Results 
     3.1 Effect of Composition 5 on Leucocyte Reduction in Mice Induced by Cyclophosphamide 
       [0170]    Test results are shown in Tables 1 and 2. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 5 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 5 low-dose group, indicating that Composition 5 can resist leucocyte reduction in mice induced by cyclophosphamide and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Effect of Composition 5 on peripheral leucocyte reduction  
               
               
                 in mice induced by cyclophosphamide (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g 
                   
                   
                   
                   
               
               
                   
                 crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 9.10 ± 1.77 
                 9.39 ± 2.08 
                 9.55 ± 2.20 
               
               
                 Model control group 
                  0.0 
                 10 
                 9.23 ± 2.03 
                 5.52 ± 1.54** 
                 5.88 ± 1.42** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 9.52 ± 2.16 
                 5.73 ± 1.60** 
                 7.89 ± 1.60 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 9.44 ± 2.20 
                 5.29 ± 1.53** 
                 7.30 ± 1.75 
               
               
                 Test drug medium-dose group  
                  4.0 
                 10 
                 9.09 ± 2.05 
                 5.37 ± 1.72** 
                 7.64 ± 2.12 ▴   
               
               
                 Test drug high-dose group  
                 12.0 
                 10 
                 9.31 ± 2.17 
                 5.67 ± 1.80** 
                 8.23 ± 1.76 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Effect of Composition 5 on the contents of bone marrow  
               
               
                 BCM and DNA in mice injected with cyclophosphamide (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 16.95 ± 2.67 
                 0.985 ± 0.225 
               
               
                 Model control group 
                  0.0 
                 10 
                 12.03 ± 2.40** 
                 0.570 ± 0.147** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 14.86 ± 2.28 ▴   
                 0.755 ± 0.128 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 13.78 ± 2.17 
                 0.681 ± 0.133 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 14.56 ± 2.23 ▴   
                 0.779 ± 0.151 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 15.13 ± 1.99 ▴▴   
                 0.780 ± 0.165 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.2 Effect of Composition 5 on Leucocyte Reduction in Mice Induced by Cytarabine 
       [0171]    Test results are shown in Tables 3 and 4. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 5 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 5 low-dose group, indicating that Composition 5 can resist leucocyte induction in mice induced by cytarabine and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Effect of Composition 5 on peripheral leucocyte reduction 
               
               
                 in mice induced by cytarabine (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude  
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 8.77 ± 1.88 
                 8.90 ± 1.74 
                 8.86 ± 1.69 
               
               
                 Model control group 
                  0.0 
                 10 
                 8.59 ± 1.61 
                 4.77 ± 1.80** 
                 4.89 ± 1.38** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 8.44 ± 1.74 
                 4.53 ± 1.28** 
                 7.54 ± 1.62 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 8.76 ± 1.83 
                 4.61 ± 1.63** 
                 5.90 ± 1.34 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 8.81 ± 1.75 
                 4.82 ± 1.36** 
                 7.23 ± 1.45 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 8.65 ± 1.60 
                 4.74 ± 1.53** 
                 7.62 ± 1.50 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Effect of Composition 5 on the contents of bone marrow  
               
               
                 BCM and DNA in mice injected with cytarabine (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 16.24 ± 2.70 
                 1.029 ± 0.218 
               
               
                 Model control group 
                  0.0 
                 10 
                 10.05 ± 2.64** 
                 0.654 ± 0.139** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.25 ± 2.31 ▴   
                 0.856 ± 0.150 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 12.58 ± 2.28 
                 0.788 ± 0.177 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 13.61 ± 2.65 ▴   
                 0.845 ± 0.158 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.52 ± 2.53 ▴▴   
                 0.921 ± 0.172 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.3 Effect of Composition 5 on Leucocyte Reduction in Mice Induced by X-Ray Radiation 
       [0172]    Test results are shown in Tables 5 and 6. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 5 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 5 low-dose group, indicating that Composition 5 can resist leucocyte induction in mice induced by X-ray radiation and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Effect of Composition 5 on peripheral leucocyte reduction  
               
               
                 in mice induced by X-ray radiation (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC on day 8 
                 WBC on day 15 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 after treatment 
                 after treatment 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 9.04 ± 2.10 
                 9.24 ± 1.76 
                 9.18 ± 2.02 
               
               
                 Model control group 
                  0.0 
                 10 
                 9.13 ± 2.25 
                 5.56 ± 1.31** 
                 5.72 ± 1.66** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 9.09 ± 2.08 
                 5.32 ± 1.54** 
                 7.80 ± 1.43 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 9.21 ± 1.90 
                 5.62 ± 1.26** 
                 6.90 ± 1.45 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 9.08 ± 2.12 
                 5.61 ± 1.30** 
                 7.53 ± 1.28 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 9.26 ± 2.33 
                 5.48 ± 1.29** 
                 8.10 ± 1.47 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Effect of Composition 5 on the contents of bone marrow  
               
               
                 BCM and DNA in mice after X-ray radiation (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow  
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 BCM (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 16.53 ± 2.96 
                 0.105 ± 0.201 
               
               
                 Model control group 
                  0.0 
                 10 
                 11.66 ± 2.03** 
                 0.570 ± 0.118** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.92 ± 2.57 ▴   
                 0.795 ± 0.127 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 13.50 ± 2.42 
                 0.678 ± 0.145 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 14.71 ± 2.60 ▴▴   
                 0.786 ± 0.132 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 15.02 ± 2.33 ▴▴   
                 0.810 ± 0.135 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       4. Conclusion 
       [0173]    The animal experiments demonstrate that Composition 5 obtained in Example 5 can resist leucocyte reduction in mice induced by cyclophosphamide, cytarabine and X-ray radiation and can improve the hematopoietic function of the bone marrow thereof, indicating that Composition 5 may be effective in preventing and treating leucopenia induced by radiotherapy and chemotherapy. 
       Example 56 
     Animal Experiment Report of Composition 6 Obtained in Example 6 in Prevention and Treatment of Leucopenia Induced by Radiotherapy or Chemotherapy 
     1. Materials and Methods 
     1.1 Sources of Samples 
       [0174]    The test drug was Composition 6 (Radix Panacis Quinquefolii, Ganoderma, fermented  Cordyceps sinensis  powder, Cordyceps, and Flos Rosae Rugosae) provided by Jiangzhong Pharmaceutical Co. Ltd. as composite powder. 1 g dry composite powder was equivalent to 13.78 g total crude drugs. 
       1.2 Laboratory Animals 
       [0175]    The same as in Example 51. 
       1.3 Primary Reagents 
       [0176]    The same as in Example 51. 
       1.4 Primary Instruments 
       [0177]    The same as in Example 51. 
       2. Experimental Methods 
     2.1 Effect of Composition 6 on Leucocyte Reduction in Mice Induced by Cyclophosphamide [1]   
     2.1.1 Grouping, Modeling and Dosage Regime 
       [0178]    Mice were randomly divided into 6 groups with 10 animals per group, i.e., normal control group, model control group, positive control group, and groups on low, medium and high doses of Composition 6. The low-, medium- and high-dose groups were intragastrically given Composition 6 at a dose of 2.0 g crude drug/kg, 4.0 g crude drug/kg, and 12.0 g crude drug/kg, respectively; the positive control group was intragastrically given batyl alcohol (100 mg/kg) at a dose of 0.1 ml/10 g body weight; the normal control group and the model control group were intragastrically given an equivalent volume of distilled water; and the dosage regime lasted for 15 days with one dose per day. From day 9 of intragastrical administration, all mice in each group, except the normal control group, were given cyclophosphamide at a dose of 40 mg/kg each day via subcutaneous injection for 3 consecutive days. One hour after the intragastrical administration on day 15, blood and femurs were harvested for tests. 
       2.1.2 Peripheral Leucocyte Assay 
       [0179]    The same as in Example 51. 
       2.1.3 Bone Marrow Nucleated Cell Count (BMC) 
       [0180]    The same as in Example 51. 
       2.1.4 Bone Marrow DNA Content Determination 
       [0181]    The same as in Example 51. 
       2.2 Effect of Composition 6 on Leucocyte Reduction in Mice Induced by Cytarabine [2]   
     2.2.1 Grouping, Modeling and Dosage Regime 
       [0182]    The same as in Example 51. 
       2.2.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0183]    The same as in 2.1. 
       2.3 Effect of Composition 6 on Leucocyte Reduction in Mice Induced by X-Ray Radiation [3]   
     2.3.1 Grouping, Modeling and Dosage Regime 
       [0184]    The same as in Example 51. 
       2.3.2 Peripheral Leucocyte Assay, Bone Marrow Nucleated Cell Count, and Bone Marrow DNA Content Determination 
       [0185]    The same as in 2.1. 
       2.4 Statistic Method 
       [0186]    The same as in Example 51. 
       3. Results 
     3.1 Effect of Composition 6 on Leucocyte Reduction in Mice Induced by Cyclophosphamide 
       [0187]    Test results are shown in Tables 1 and 2. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 6 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 6 low-dose group, indicating that Composition 6 can resist leucocyte reduction in mice induced by cyclophosphamide and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Effect of Composition 6 on peripheral leucocyte reduction  
               
               
                 in mice induced by cyclophosphamide (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 10.60 ± 1.95 
                 10.42 ± 2.30 
                 10.54 ± 2.27 
               
               
                 Model control group 
                  0.0 
                 10 
                 10.44 ± 2.23 
                  6.08 ± 1.88** 
                  6.31 ± 1.67** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 10.58 ± 2.34 
                  6.25 ± 1.76** 
                  8.96 ± 1.93 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 10.72 ± 2.28 
                  6.16 ± 1.93** 
                  7.58 ± 1.75 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 10.28 ± 2.19 
                  6.40 ± 1.97** 
                  8.22 ± 1.76 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 10.47 ± 2.23 
                  6.27 ± 1.73** 
                  9.40 ± 1.94 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Effect of Composition 6 on the contents of bone marrow  
               
               
                 BCM and DNA in mice injected with cyclophosphamide (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 17.58 ± 2.81 
                 0.116 ± 0.212 
               
               
                 Model control group 
                  0.0 
                 10 
                 12.67 ± 2.52** 
                 0.745 ± 0.156** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 15.72 ± 2.34 ▴   
                 0.958 ± 0.138 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 14.33 ± 2.76 
                 0.842 ± 0.147 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 15.28 ± 2.19 ▴   
                 0.915 ± 0.165 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 16.20 ± 2.59 ▴▴   
                 0.969 ± 0.173 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.2 Effect of Composition 6 on Leucocyte Reduction in Mice Induced by Cytarabine 
       [0188]    Test results are shown in Tables 3 and 4. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 6 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 6 low-dose group, indicating that Composition 6 can resist leucocyte induction in mice induced by cytarabine and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Effect of Composition 6 on peripheral leucocyte  
               
               
                 reduction in mice induced by cytarabine (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 WBC before 
                 WBC after 
                 WBC after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 modeling 
                 treatment 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 10.14 ± 2.20 
                 10.32 ± 2.66 
                 10.44 ± 2.70 
               
               
                 Model control group 
                  0.0 
                 10 
                 10.25 ± 2.25 
                  6.45 ± 1.39** 
                  6.15 ± 1.34** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 10.10 ± 2.40 
                  6.62 ± 1.52** 
                  8.52 ± 1.55 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 10.33 ± 2.18 
                  6.77 ± 1.70** 
                  7.48 ± 1.70 
               
               
                 Test drug medium-dose group  
                  4.0 
                 10 
                 10.51 ± 2.23 
                  6.38 ± 1.54** 
                  8.45 ± 1.66 ▴▴   
               
               
                 Test drug high-dose group  
                 12.0 
                 10 
                 10.44 ± 2.16 
                  6.50 ± 1.61** 
                  8.62 ± 1.47 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Effect of Composition 6 on the contents of bone marrow  
               
               
                 BCM and DNA in mice injected with cytarabine (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 15.89 ± 2.70 
                 0.973 ± 0.209 
               
               
                 Model control group 
                  0.0 
                 10 
                  9.66 ± 2.05** 
                 0.625 ± 0.143** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 13.58 ± 2.17 ▴   
                 0.830 ± 0.116 ▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 11.87 ± 2.64 
                 0.756 ± 0.170 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 12.30 ± 2.38 ▴   
                 0.818 ± 0.180 ▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.19 ± 2.49 ▴▴   
                 0.900 ± 0.166 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       3.3 Effect of Composition 6 on Leucocyte Reduction in Mice Induced by X-Ray Radiation 
       [0189]    Test results are shown in Tables 5 and 6. The number of peripheral WBC and the contents of bone marrow nucleated cells and DNA in the model control group decreased as compared to those in the normal control group, suggesting a successful modeling. Compared to the model control group, the number of peripheral WBC and the contents of bone marrow nucleated cells and DNA significantly increased in the Composition 6 high- and medium-dose groups and in the positive control group, and also showed a tendency to increase in the Composition 6 low-dose group, indicating that Composition 6 can resist leucocyte induction in mice induced by X-ray radiation and improve the hematopoietic function of the bone marrow thereof. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Effect of Composition 6 on peripheral leucocyte reduction  
               
               
                 in mice induced by X-ray radiation (  x  ± s, ×10 9 ) 
               
             
          
           
               
                   
                 Dose (g 
                 Number  
                 WBC 
                 WBC on  
                 WBC on  
               
               
                   
                 crude 
                 of 
                 before 
                 day 8 after 
                 day 15 after 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 modeling 
                 treatment 
                 treatment 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 9.88 ± 2.16 
                 9.66 ± 1.76 
                 9.78 ± 2.43 
               
               
                 Model control group 
                  0.0 
                 10 
                 9.72 ± 2.09 
                 5.90 ± 1.55** 
                 5.70 ± 1.82** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 9.65 ± 2.22 
                 5.53 ± 1.28** 
                 8.03 ± 1.59 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 9.84 ± 1.70 
                 5.76 ± 1.44** 
                 7.24 ± 1.60 
               
               
                 Test drug medium-dose group  
                  4.0 
                 10 
                 9.73 ± 2.26 
                 5.81 ± 1.39** 
                 7.90 ± 1.44 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 9.55 ± 2.01 
                 5.72 ± 1.70** 
                 8.34 ± 1.75 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Effect of Composition 6 on the contents of bone marrow  
               
               
                 BCM and DNA in mice after X-ray radiation (  x  ± s) 
               
             
          
           
               
                   
                 Dose (g crude 
                 Number of 
                 Bone marrow BCM 
                 Bone marrow 
               
               
                 Groups 
                 drug/kg) 
                 animals 
                 (×10 6 ) 
                 DNA 
               
               
                   
               
               
                 Normal control group 
                  0.0 
                 10 
                 16.26 ± 2.78 
                 0.996 ± 0.222 
               
               
                 Model control group 
                  0.0 
                 10 
                 11.41 ± 2.62** 
                 0.563 ± 0.121** 
               
               
                 Positive control group 
                 100 mg 
                 10 
                 14.78 ± 2.44 ▴▴   
                 0.780 ± 0.135 ▴▴   
               
               
                 Test drug low-dose group 
                  2.0 
                 10 
                 13.02 ± 2.55 
                 0.665 ± 0.160 
               
               
                 Test drug medium-dose group 
                  4.0 
                 10 
                 14.35 ± 2.32 ▴▴   
                 0.772 ± 0.144 ▴▴   
               
               
                 Test drug high-dose group 
                 12.0 
                 10 
                 14.66 ± 2.09 ▴▴   
                 0.801 ± 0.127 ▴▴   
               
               
                   
               
               
                 *P &lt; 0.05, 
               
               
                 **P &lt; 0.01 vs. normal control group; 
               
               
                   ▴ P &lt; 0.05, 
               
               
                   ▴▴ P &lt; 0.01 vs. model control group. 
               
             
          
         
       
     
       4. Conclusion 
       [0190]    The animal experiments demonstrate that Composition 6 obtained in Example 6 can resist leucocyte reduction in mice induced by cyclophosphamide, cytarabine and X-ray radiation and can improve the hematopoietic function of the bone marrow thereof, indicating that Composition 6 may be effective in preventing and treating leucopenia induced by radiotherapy and chemotherapy.