 sistersville is a city in tyler county, west virginia, united states, along the ohio river. the population was 1,396 at the 2010 census. the sistersville ferry crosses the ohio river to the unincorporated community of fly in monroe county, ohio.{{cite book | url=http://books.google.com/books?id=-cwfn4ul8l8c&lpg=pp1&pg=pa28#v=onepage&q&f=false | title=ohio river guidebook | publisher=inland waterways books | date=2008 | accessdate=22 nove
Cities_in_Tyler_County,_West_Virginia Cities_in_West_Virginia West_Virginia_populated_places_on_the_Ohio_River